LOCUS       DAOSME010000001        85943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_327_length_85943_cov_22.486843, whole genome shotgun sequence.
ACCESSION   DAOSME010000001 DAOSME010000000
VERSION     DAOSME010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 85943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85943
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_85943_cov_22.486843"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PLG98_00005"
     CDS             <1..980
                     /locus_tag="PLG98_00005"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HOE31657.1"
                     /translation="RLGANEAPPAIISVFLGDQLIQILENIQRGTASKATDAQIIDLG
                     ISSLYQVSKDNTDRNRTSPFAFTGNKFEFRAVGSSQSISTQIIALNTIVAESIDDIAE
                     KIIARKDSKNIYQAVMDVLKDEITTIKPVLFNGDNYSKEWEAEAARRGLPNEKTTPTA
                     LKALITKKALNLFEKYEVLSNIELKSRQLIHIERYIKDLEIEVKCLNSMCMSYILPAA
                     VIYQKRLAGAINATREALGTEAVVSAEIELLKKISDLINNIFATNRDIQQKVESANAL
                     HDEQKKAEMLSAKVKPMMEELREYADALEILVDDDIWPVPKFWEMLFIC"
     gene            1052..2182
                     /gene="carA"
                     /locus_tag="PLG98_00010"
     CDS             1052..2182
                     /gene="carA"
                     /locus_tag="PLG98_00010"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOE31658.1"
                     /translation="MFQLGKKRKALLVLEDGSIFAGNAFAGSGETMGEVVFNTGMTGY
                     QEVITDPSYKGQIVTMTYPLIGNYGINDEDRESAGIHLEGFIVKEYQAHPSNWRMTKS
                     LKSFLDDHGKIGIEGIDTRALTRRLRMAGAMKGIISTESANLESLLATLRDYPGLVGR
                     DLVREVTCSTPYQWERNAVRADGIAPDKPQHKLRVAVVDCGVKYNILRLLEKNGCEVV
                     VMPAFSTGDDIMSLKPDGVLFSNGPGDPAALSYIVDTARFIIGKVPVFGICLGHQIIG
                     QAIGGCTEKLKFGHHGINQPVKNAETGRVEITSQNHGFVVVLESMNDQAQKTYHNLND
                     NTSEGMSMPQAFSVQYHPEAAPGPHDTAYLFGRFVEMMKSVK"
     gene            2183..5389
                     /gene="carB"
                     /locus_tag="PLG98_00015"
     CDS             2183..5389
                     /gene="carB"
                     /locus_tag="PLG98_00015"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOE31659.1"
                     /translation="MPKRNDIKKILLIGSGPIIISQACEFDYSGTQACKALREEGYEV
                     VLINSNPATIMTDPEMADRTYVEPITPEAVEKIIARELPDALLPTLGGQTGLNTAVTL
                     AESGVLKKYNVEMIGASLDVIHKAEDREKFRHAIEKIGLRMPKSGFARSMQDVLKIAD
                     DIGFPIIVRASFTLGGTGSGVAYNREELMEIAKAGLDASMISEVMIEESVLGWKEYEL
                     EVMRDKADNVVIICSIENFDAMGVHTGESITVAPAQTLTDKEYQNMRDAAIAIMREIG
                     VETGGSNVQFAVHPQTGELIVVEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETMASFEPTIDYCVVKIPRWTFEKFPQAEDFLTTSMKSVGETMAIGRTFKE
                     SLQKAVRSLEIGRFSLEQALPDHIPDKKEFLKSKLIKPNSLRLFYIAAAMQNGLSIEE
                     LYRLTKIDPWFLHQIREIVDAENEIKESTPSVEVLREAKKMGFSDRALASFWKMTEPQ
                     IRQWREKGKIIPVYKLVDTCAAEFEAYTPYYYSTYETENETRLSDKRKVAIIGGGPNR
                     IGQGIEFDYCCVHASFALREEGVESIMINSNPETVSTDYDTSDKLFFEPVTLEDVLHI
                     LQTEKPEGVIVQFGGQTPLNLAKGLETAGINILGTSPDAIDRAENRDRFQEIVTKLKL
                     NQPDNDTARDTQKALQAAERIGYPVLVRPSYVLGGRSMEIVYDEETLKSFMEKALLVS
                     PEHPILIDKFLEDAVEIDVDAVSDGETTVVAGIMEHIEEAGIHSGDSACMLPPLTIPG
                     DVLDLIRKQTTMLAKELNVIGLMNIQYAVKDGKLYVLEVNPRASRTVPFVSKAIGVPL
                     AKLATKVMLGKSLKELGFTESPRPKHISVKEAVFPFNRFPDVDILLGPEMKSTGEVMG
                     IDQSFGLAFAKSQMATGFKIPQQGTVFISVNDQHKDRIVTVAKTFQKIGFKILATKGT
                     ALYLLEHGISAGVVLKLSEGRPNIVDFIKNGDMQIVINTSIGRKSSRDAYHIRHSALI
                     YNILYTTTIAGARALAEATVAIKEKAWQVCSLQEYQKA"
     gene            5545..6966
                     /gene="purF"
                     /locus_tag="PLG98_00020"
     CDS             5545..6966
                     /gene="purF"
                     /locus_tag="PLG98_00020"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOE31660.1"
                     /translation="MLSEKEQEKRYYPREECGIFATYGHEEASRVAFYGLFALQHRGQ
                     ESAGIVSSDGCNVYEHKGMGLAAEVFKEETLQKLPGKLAIGHVRYSTTGSSNLCNAQP
                     FLVHYGNEYYALAHNGNILNAQKLRRELEDSGSIFQSTMDSEVIIHLMAHYLKQGLEK
                     ALAAALSRLEGAYCLIMLTRDKIIAARDPRGFRPLALGKVSGKWVIASETCAFDLVGA
                     DYVRDVEPGEILIIDATGVKSVNPFSAIRHCHCIFELIYFARPDSQVFRQNVYQYRKR
                     LGHELAREYKPDVDIVMPFPDSGVYAALGYAEESGRPFEMGMIRNHYIGRTFIQPSQA
                     MRDFGVRVKLNPVRALLENKKILIVEDSIIRGTTSRNRVKNLRNIGIKAIHMAVSCPP
                     TVYPCPYGIDFSSKGELVASKMESEKAIADFIGLDSIHYLSVEGMIRATGLKKDCFCL
                     ACFTGHYPIAPPEILDKFCLETK"
     gene            7234..7680
                     /locus_tag="PLG98_00025"
     CDS             7234..7680
                     /locus_tag="PLG98_00025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOE31661.1"
                     /translation="MGIHTYKAASVLKETGMMVESDARGFKFIADEPRGLGGTNRGMN
                     PMETILCALGACQCMTARFFAMLLKIDLTEYHVDVEGDIDARGFLNADSGIRPGFQEI
                     RLTVRMKANAPEEKIAELLAMVESRCPVGETVLHGAPVVVRHIELL"
     gene            complement(7940..9691)
                     /locus_tag="PLG98_00030"
     CDS             complement(7940..9691)
                     /locus_tag="PLG98_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31662.1"
                     /translation="MAIDYSVIGNKIIMRTGGRLCETPDELLESDLFFRILTECIREL
                     ESRNSLLLNIFGKTTVDQQTIQKMALVLKNLAKIEAELIPKVVEGTEPYFSDINNLYK
                     FVEYLYNFWRSHERYIIIDSFDDSFEKRPYRVFNQRIQGLTELIRDTYRDIQENITGS
                     HPRIYRQLSAGAKISAICTREDIHLSGVYADKLSSVPMIRQVLFYPTLIFDPPMNKRT
                     GQFVRIDTNPMDVVDIRPDEWLCYPAMVGNLLMHVYFHQNFMDLGFSLCNLFELAGDK
                     HLAKKPDAIFLFGVQDGSLDGLAQYPTMFHEDRSNNLLIGACPGSSEFGYFGYLKKMM
                     LTLHNAIMIRQGRLPFHGALYQIHLKNGTEATILMMGDSGAGKSETLEAFRMLGDEHL
                     RGLQIIADDMGSINITEQGDIIGYGTETGAFIRLDDIKPGYAFRNIDRSIIMSPGKTN
                     ARIVLPVSTLEEVNMGVGIEYVLYANNYEEIDDDHPALERFSTPDQAFSVFREGTVMS
                     KGTTTSKGIVHSYFANIFGPPQYRKIYDQLARKSFEAFFRKGIFVGQIRTRLGIPGCE
                     MTGPKEAALKLLDMILA"
     gene            10076..11437
                     /locus_tag="PLG98_00035"
     CDS             10076..11437
                     /locus_tag="PLG98_00035"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOE31663.1"
                     /translation="MKEGIVCKFGGTSVANAAQINKVKNIVTMNTKRQYVIVSAPGRD
                     SSDDQKVTDHLLNVATNGRHFRLQNKNITGQQSYEAVLGKFQRLIDELAIDGGDILKN
                     LEKDMKTPLPDVKRLDFMASRGERYNAEVVSRYLKKQGIPSELVLPEEMGLTVTGDFS
                     NARVIPVSYRNIKKKLSSIEGVSVIPGFYGVTEKGDIAVFSRGGSDLTGGEVAFSIEA
                     GLYENWTDTDGIYRADPRLIPEAKVIPRLTYKEIRLLSSKGFDVFHYDAMVNCKKRNI
                     PIQIRNTNNTNSEGTLIVSERVPEEVVVGIARKDNIAFIYIERDGAGETIGFVHDLLS
                     VIKDYGIETYHYPTDRDDMTILINQDDMVGYAEDIKERIEREFKPDTLEFNYNISILS
                     PVGIGMKNHPGVIAEAANALRDKNINIDIIIQGPAQISFHFGFQNFYADSALRAMYNC
                     LIE"
     gene            complement(11442..11594)
                     /locus_tag="PLG98_00040"
     CDS             complement(11442..11594)
                     /locus_tag="PLG98_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31664.1"
                     /translation="MPLPVAAYQRRPQDSDYYHCVEDYFETFATETATPVIAGPKSLH
                     FISYHH"
     gene            11784..14372
                     /gene="leuS"
                     /locus_tag="PLG98_00045"
     CDS             11784..14372
                     /gene="leuS"
                     /locus_tag="PLG98_00045"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOE31665.1"
                     /translation="MKYEPLVIEEKWQKKWEDEKSFKVTEDPQKKKYYLLEMFPYPSG
                     KIHIGHVRNYTIGDVVARYKRMKGFNVLHPIGWDAFGMPAENAAIEHKIHPAKWTNEN
                     IEHMKKQLKRMGFSYDWDREVSTCEPKYYRWEQLFFIWMYEKGLAYKKRSTVNFCTKC
                     DTVLANEQVEGGQCWRCGTEVQEKVLDQWFFKITAYIEELLDYCDKLPGWPERVMTMQ
                     KNWIGKSYGCEVDFPMADGNGAIKVFTTRQDTLFGATFMLVAAEHPLVMELTKGKAVE
                     KDVMQFVEKVKKQDKLMRTSEYYEKEGLFLDSYCLNPLTGGKMPIFAANFVLADYGTG
                     CVMAVPTHDQRDFEFAKKFNLPLIVVISPKDRTLDAATMTEAYVDEGVLVNSGQFNGM
                     ENTKVLEAIADFLEKEGKGKRTIQYRLRDWGISRQRYWGAPIPMINCAKCGIVPVDEK
                     DLPVILPENVKLSTEGGSPLAALKEFVETTCPKCKGPASRETDTMDTFVESSWYFDRY
                     CCPDYDTKPGLDRKKLDYWMPVDQYIGGIEHAILHLLYSRFYTKVLRDFGVIGVDEPF
                     TNLLTQGMVCKETMKCKEHGYLFPDQAADGKCHICGKDVIIGKTEKMSKSLKNVVDPD
                     YLVKKYGADTARIFCLFAAPPEKDLEWSDQGVDGSFRFLGRLWRIVDEYLEDIKGVAP
                     ANGALQLEGDLKSLRRKTHQTIRKVSIDIEDRFHFNTAISAVMELVNVVYQVKRPASS
                     DKVALSVVREALEAAIILLVPIVPHVTEELWQLLGHQNLASDMSWPEFDPAIASEEEM
                     TIVVQINGKLRSRMTVPVDESEEKIKADALADEKIKSMTAGMQIKKVIYVPKKLVNIV
                     VTLSES"
     gene            14561..15094
                     /locus_tag="PLG98_00050"
     CDS             14561..15094
                     /locus_tag="PLG98_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOE31666.1"
                     /translation="MKNEIGLITKIAIICISCAVLFGCGYSFAPRGESIDTRIQKVYV
                     EPFENNTAQAEIENYVRSAFINQFIQNSRFKLASGTETADAVVRGKIVNLHTSSLTHL
                     KNDLAAEERATMIMEVVFEDKSNGRIIWSAKNITDSVDYVLNEDINMLPASKKQAFMK
                     LADDMAEKAFNLMMSGF"
     assembly_gap    15141..15240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15484..17871
                     /locus_tag="PLG98_00055"
     CDS             15484..17871
                     /locus_tag="PLG98_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOE31667.1"
                     /translation="MTPSARQFGTFFLLPFTDVQEALDFISSNKRSVTVIKNLSAYLA
                     RLSCKYLLLVDAEKDISIRESSLRHFLKPFTDQHTGMVYSDFTSKKRNLLKKHPVIDY
                     QAGSIREDFNFGSLLIFSVDAVRTVLQKYGASPSDPDVALYDLRLKTSADYHIRHVRK
                     FLYTVSVKNHKQLAASGGKAETHFAYVAKENFLRQKKLEKIATHHLKRIGAYLPSRTK
                     IVNNEQDGGQWKASIVIPVRNRKKMIADALKSALKQKTDFPFNVIVVDNHSTDGTTDI
                     LRKFASTYPHIHHLIPSRRDLGIGGCWNGAIYSPYCGRYVVQLDSDDLYSSPHTLQKV
                     VNKLRRGRYAMVVGSYTIVDEHLKKIPPGLIAHKEWTQTNGHNNLLRVNGMGAPRAFD
                     RSVICRTGFPNVSYGEDYAVALRITREYKIGRIYENLYWCRRWKDNTDAGLDAEKKNR
                     NDFYKDQLRTLEIEARKSANKGDACVNSRRVLAEFSGGKALALPFLCQHLYDAQKKTW
                     PKLAEACRNLASVHKRKLPGAYDVYLQYNPARAISSGASVDAQSIRQRPCFLCQENLP
                     VEQKGILYRSQYLILCNPAPIFEKHFTIVSLQHTLQEITSSINGFLQLSADMSGYAVF
                     YNGPACGASAPDHLHFQAVPKKFLPFLREIKKLPVCDTSSLRYVSGETFDRSVVLLES
                     GDQDALSMQFETLLKALQSIMQTKVEPMVNVVCDHAGHRWRLIVFLRQKHRPDAYFEK
                     GEKRIFISPGAVDMAGVVITPLLDNYNRLDYNAVREIYRDVSLSENTTDAVIRKL"
     gene            17886..18440
                     /locus_tag="PLG98_00060"
     CDS             17886..18440
                     /locus_tag="PLG98_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOE31668.1"
                     /translation="MFFAEEAIVDSAMSFSEAIEGTTAPDEIILSLSLVDVFYYSFDG
                     RKHQGQMLISKELEDDAYDIFNVIEKIFFPVDKVIPITAYQWNDYRSMADNNTSSFNF
                     RVIEGTTKLSMHSLGKALDINPVQNPVIYPNGVVAPEGAKYQPDERGTFSADHPIVRE
                     FLKRGWHWGGNFNQPKDYHHFEKP"
     gene            complement(18504..19238)
                     /locus_tag="PLG98_00065"
     CDS             complement(18504..19238)
                     /locus_tag="PLG98_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HOE31669.1"
                     /translation="MNESTSDQAALGDLIVDIGRLFIGKPYRDGMLENPGKEKLIINV
                     SAFDCTTFVETVLAFFRCAASGRFSSGAFKKYLKLIRYRGGKISGYSSRLHYFTDWMR
                     NNIEKKFLIDITGHLQATPHRKKIDFMSTHPELYPALKNKVQLAKIIDIEKMLSRKAF
                     PVMNKEKLGTRIKKIQSGDVIAFASNQQGLDVAHVGFALWQGNSLRLLHASRQEGKVV
                     ISKKTLLAYLTSNKKFTGIIVARSVF"
     gene            complement(19513..19773)
                     /gene="rpsT"
                     /locus_tag="PLG98_00070"
     CDS             complement(19513..19773)
                     /gene="rpsT"
                     /locus_tag="PLG98_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOE31670.1"
                     /translation="MATHKSAEKRDRQNKKHRQRNIAAKSALKTKIKSVVAGVESKNK
                     ENSAKALKAAIPAIAKAGAKGLIHKKNASRKISRLTKKVNAM"
     gene            20093..21799
                     /locus_tag="PLG98_00075"
     CDS             20093..21799
                     /locus_tag="PLG98_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE31671.1"
                     /translation="MTIAVKQQSGKKSINKRKVLQNDLSILARDIIANAGVGMYIVQD
                     GKFVYVSELYQKITGFTEEELIGTYPLDNIHPDDMKTVREKAIKCLKEGNCNPYEYRF
                     ITKSGEIIWVLEAVTSISFKGERAALGSFINITERKLIEKKLHHEEQLFRTLTDQSSD
                     IIAIIDRKGVITYENKAIEPVLGLKVDEQMGGKGFDNLHPDDVPFVSNELKRLFADPD
                     ASASRSEVRLRHKNGQWLVFEFMASGLVNNNVVEAVMINLRDITERKRAEESLRFSEE
                     KYRTILENIQEGYFEVDLAGNLTFYNDPVCEIMGYTREELMSMNYKKYTGNEMAVKVY
                     QAFNKVYLSGNSTKEIDWQIIRKDGARRYIEASVSLQKDFSGHPKGFKGIIRDITERK
                     EMDQKLNHLATHDALTGLPNRILFMDRLQVALVQSRRNRNKLAVMMLDLDHFKDVNDT
                     LGHLAGDKLLQEVGNRLTDILRHNDTVARLGGDEFVVLLSDLERMEYAAGVAKVILKA
                     FGKPFLLADHEITPNVSIGIAFFPDDCDDVESLLQKSDMAMYSVKTHGRNSYKFYSHI
                     AS"
     gene            complement(21813..23423)
                     /locus_tag="PLG98_00080"
     CDS             complement(21813..23423)
                     /locus_tag="PLG98_00080"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOE31672.1"
                     /translation="MKHTKFIFITGGVLSSLGKGLAAASISALLESRGLKVTNQKLDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFCSTCMGKSNNLTTGQVYYAVIT
                     KERRGDYLGKTVQVIPHITNEIKDFIRDTADGFDVAIVEIGGTVGDIESLPFLEAIRQ
                     FRNEVGRENAIFIHLTWVPLIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTENFLS
                     DDIKSKIALFCNVEVHSVFTAKDVSCIYEVPLIFHREGLDAKIVDLLNIWTGQPKLDV
                     WEDVVDKFHNPLHDVTIGIVGKYVNLTDSYKSLNEALVHGGIANNCRVKLKFIDSEKI
                     EAEGLGKHLDDIDAVLVPGGFGIRGIEGMILAIQRAREKKIPFLGICLGMQMAVVEYA
                     RNVCKLEKANSSEFDPETSYPVIDLLPDQRHVKEKGASMRLGGWPCLVEADSFACKAY
                     GQGEISERHRHRYEFNNDYKEALMNQGLRITGISPDGRLAEIVEIKDHPWFLGCQFHP
                     EFKSRPIKPHPLFSKFIEAALNYSRSKT"
     gene            complement(23470..24429)
                     /locus_tag="PLG98_00085"
     CDS             complement(23470..24429)
                     /locus_tag="PLG98_00085"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOE31673.1"
                     /translation="MGTTIWVTKDFAHMSDVASGIVIKKSIELLKRKKEIALGLATGN
                     SPTGLYKHLAKAVNEGRLDAGRIRSFNLDEYVGLPGNNIQQRVMHPESYAYFMIQELF
                     GHLNKKFIETRVPYGSLIDQKMLIRELKDNKSDWEFKGTDAGKSIAIKAKAQSPYLAW
                     IRKDILDGYVRKIKAAGGIDLQIIGVGGRGHVAFHESGIPFQGSSMLLVKLDDNTIAN
                     AVADGHFKTKADSPQYAISMGAELVYKAKTVVLVANGARKTESVTRSILGNMTPEIPI
                     SYGQAYSQKGGELIYVLDRIAAQGLLENTSALKIRGVILKNLA"
     gene            complement(24448..25797)
                     /gene="glmM"
                     /locus_tag="PLG98_00090"
     CDS             complement(24448..25797)
                     /gene="glmM"
                     /locus_tag="PLG98_00090"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOE31674.1"
                     /translation="MGKLFGTDGIRGEANRHPMTPQIAFGVGQAIAHVFKKGGHRSVI
                     IIGKDTRLSGYMLESSLEAGITSMGGYSYLVGVMPTPGIAFVTQSMRADAGVVISASH
                     NPFQDNGLKIFGGDGYKLTDEKEDIIEDLILNDKLSEFLSKGDDIGRAFRLEGVNGRY
                     IVFAKNTFPRNLSLEGMKIVLDTANGATYRVAPDVFHELGADIDVIHKTPNGLNINDQ
                     CGSQHTERLKKTVLENGAAIGLAFDGDGDRLIAVDEKGQEITGDQILVICANVLKREG
                     RLKNDLLVSTVMSNLGLKVACKQYGFKHHASKVGDRYVLEDMLKMGSVIGGEEAGHMI
                     FLDHHTTGDGIIAALQLVAAMIKENKPLSELARMMDIFPQKLINVDVKSKPDIDQIPR
                     LAEAIKQVEKELGDEGRVLVRYSGTQNMCRVMVEGPTDAVTLKYCRQLADIIKSEIG"
     gene            complement(25845..27110)
                     /locus_tag="PLG98_00095"
     CDS             complement(25845..27110)
                     /locus_tag="PLG98_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOE31675.1"
                     /translation="MDLKPRSLKKINDLLERGVSIPNPETIDIGDDVAIDRISGKGVK
                     IYPGCRIYGKETTISSGCQLGYEAPVTIENCQLAEKVELKGGFFSKSVFLEKSNMGMG
                     AHVREGCILEEEAGGAHCVGLKQTILFPFVTLGSLINFCDCLMGGGTSRSNHSEVGSS
                     YIHFNFTPDADKTTPSLLGDVPRGVMLNQPPIFLGGQGGIIGPVRLGFGNVVAAGSVL
                     RHDYPGDNQLIFEKAPACSVKPFYPAAYPHLERIVENNILYLANLKALEAWYTHVRKP
                     FLETGEYGSFLYAGVMEQLSSARKERLKRLKAMAQKAAAAAEEEKNPERTCARGRKEL
                     HEKMGHIEDLCNGKASPASGKTDELRDTFLNDLESIAGKNKTNYIETIQALPAGISSK
                     GVMWLDGIVRDLGENVSRAVPKLGLLKTF"
     gene            complement(27160..28515)
                     /gene="gdhA"
                     /locus_tag="PLG98_00100"
     CDS             complement(27160..28515)
                     /gene="gdhA"
                     /locus_tag="PLG98_00100"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOE31676.1"
                     /translation="MSVIQDVIAKVKQTDPNQPEFHQAVEEVMETLEPTVKKHPEFVK
                     ANLYERIVEPERAVIFRVPWVDDKGNVQVNRGFRVQFNNAIGPFKGGIRFHPSVNLGI
                     IKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDGEVMRFCQAFMRELYRHIGADVD
                     VPAGDIGVGGREIGFMYGYYKKIRNEHTGVLTGKGMSYGGSLIRPEATGYGCVYFAAE
                     MLATRKMDFKGKTVAVSGAGNVAQYAVEKVNQLGGKVISLCDSSSCIIDEAGIDDKKC
                     SFVLDLKNVKRGRISEYANKFKSECFEGKNVWDVIREQGIKVDIALPCATQNEINGKN
                     AAALVKNGCLCVAEGANMPSTPEAIKIFQDKKILYGPGKAANAGGVATSGLEMSQNSL
                     RLLWTREEVDNRLLIIMRSIHKNCYDTAETYGKKGDYVTGANIAGFTKVANAMLAYGV
                     V"
     gene            complement(28581..29582)
                     /locus_tag="PLG98_00105"
     CDS             complement(28581..29582)
                     /locus_tag="PLG98_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOE31677.1"
                     /translation="MKHLFNPEDIERKVLLILKILNENSESAGARVIARKMKEHGVHL
                     SERTVRYHLKSMDERGLTRLIGRRDGRIITDMGMEEINNARVRDKISLSISRIDVLSL
                     KTTFDIKKRRGLVPVNISLFPQKQFRKALAVMKPVFKKKIAVSDLVAVASAGETLGEV
                     TIPEGKIGFATVCSILINGVLLKHGIPMDSKFGGILQVRHGNPLRFVELINYSGSSLD
                     PSEIFIRGKMTSVKDVVKKGEGKILANFREIPALALSLVENIVADFKKIGVNGALSIG
                     NVGNPVCQTNVDINKVGMILLGGLNPIAAAGEEGFDIENKAMSTVMEYETLQRVEDI"
     gene            complement(30011..30316)
                     /locus_tag="PLG98_00110"
     CDS             complement(30011..30316)
                     /locus_tag="PLG98_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE31678.1"
                     /translation="MPSKKKSKSEIVMNVKATPYAAVEEIMEKKLEDIATILSASGDR
                     KSKLYEEVMNIVEKGLFKIALRRSNGVKTSAAIFLGINRNTFTDKMTKLGIDCKKSK"
     gene            complement(30385..30900)
                     /locus_tag="PLG98_00115"
     CDS             complement(30385..30900)
                     /locus_tag="PLG98_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31679.1"
                     /translation="MEQNVFKIKKAFLLPFIAIIILLFVLLILNLLNGQPWEKIILAV
                     AFAVTLLVGIEAANRHITASNEGMKVKKFSRVKTLTWPDVTHLGVVAILNKAYFLLTT
                     TKGFYFFSNMYENHALLIQSIVDKLEAERVEMEVRNYLEHPRERRSLIVICWITALII
                     SAFIVLKLLSF"
     gene            complement(30955..31437)
                     /gene="moaC"
                     /locus_tag="PLG98_00120"
     CDS             complement(30955..31437)
                     /gene="moaC"
                     /locus_tag="PLG98_00120"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOE31680.1"
                     /translation="MSKKLSHINHEGNVQMVDVTAKPQTAREAKARGIVRMDAATLTL
                     ISKGRIAKGNVLTTAKIAGIMAAKKTGDLIPMCHPLELTGVDLTFTVDEKKSAIIIEA
                     SVRIAGKTGVEMEALTAVSVAALTIYDMCKAVNKKIVISDIMLLEKSGGKSGTFIRSK
                     "
     gene            31658..32431
                     /locus_tag="PLG98_00125"
     CDS             31658..32431
                     /locus_tag="PLG98_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOE31681.1"
                     /translation="MAALRVRVKDSTDKHKILSLLNKLHPVSCDKPLIRLGAFGDGGY
                     LVPDDLTGIEACFSPGVGFSSVFEKDCADRGMKVFMADGFVNHPSLSHELFVFIKKYV
                     GNTDNDHEMTLDAWVNSSLADATGDLLLQMDIEGAEYDVLSAVSEDLLKRFRIIVIEF
                     HLLDGLRRQPFFNLASQVFEKILRTHTCVHNHPNNCSDAVKVDDIDIPMVMELTFLRN
                     DRIHNPSFVRTFPHPLDCDNTDRNPSLVLPKSWYRNHQK"
     gene            32550..34079
                     /locus_tag="PLG98_00130"
     CDS             32550..34079
                     /locus_tag="PLG98_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOE31682.1"
                     /translation="MFVKVNSMSVIGMESYPVFVEVDTSQGLPQFATVGLPDASVKES
                     RDRIKAAIKNSGYHFPRSHVTINLAPADIKKEGTGFDLPIAVGILAAEKLIREERLRD
                     CLFVGELSLDGSIKRVNGLLSVAFKALETGLTSVFVPEENAPEAAMVKGVNIIPVKTL
                     PDVVECLEGRKTIEPLCLDLEKIFHKTNQYDVDFSEIKGQKQAKRALEIAAAGGHNVL
                     MLGPPGSGKSMLAKRLPTILPDLSFEEAIDVTKVFSVAGFLDKDKTLMAVRPFRSPHH
                     TISDAGLVGGGQMPRPGEISLAHLGVLFLDELPEFRKNVLESLRQPLEDGMITITRSS
                     VTASFPAKFMLVAAMNPCPCGYFGDRVHSCRCTPQQIRQYQSKISGPLLDRIDLHIEV
                     PSVKYRALSDKETGESSAAIKQRVSNARNIQNERFGTNGIMCNARMTEKHIRQFCQVD
                     EEAHKLIDMAMEKLGLSARAVNRILKVSRTIADLEHDESIRTSHVAEAIQYRNLDRGL
                     I"
     gene            34166..35260
                     /locus_tag="PLG98_00135"
     CDS             34166..35260
                     /locus_tag="PLG98_00135"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOE31683.1"
                     /translation="MEIKIKKVPLKKRKTKPADESKLGFGKIFTDHMFTMKYREGEGW
                     YDPVIEPYHPLQLDPTAMCLHYGQEIFEGLKAYRGQDSAIYLFRPMENIKRMNASAER
                     LCMARIDEDLFMEAMKKLVLLEKDWIPHGEGTSMYIRPTMIATEAALGVHPANEYLFF
                     IVVGPVGAYYPQGFSPTKMYVSEEYVRAVRGGIGHVKAAGNYAASLYASRIATNMGYT
                     QVLWLDSVEKKYVEEVGTSNIFFVIGDELITPPLGGSILPGVTRNSVITLARYLGTNV
                     VERPISIEEVIKTSENGSLKEAFASGTAAVISPVGNIYFRDKEYAINGGKTGALAEKL
                     YNEILKIQYGMVEDPFGWRIKIANGKKRSR"
     gene            35465..36229
                     /locus_tag="PLG98_00140"
     CDS             35465..36229
                     /locus_tag="PLG98_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOE31684.1"
                     /translation="MKQDINSAKKNVPEKIDFLQKMMKFFLNRNKLFTSSWIALSVIV
                     VAISIPGSSFSEERPAIDDVVQKLQLSYESTKDLSADFIQETTVKSIKKTQREEGRVF
                     FKNPQNMSWNYTKPQGKKLVMNHQTAWLYLPQEKVAYRQSAESIFQSRLLIDFFSGSG
                     KLKDDFIITYDSAISPDKDGHYLLVLTPRQKNTTFNVLRLTIDKNNFYILRLSFDDAF
                     GNSTDLRFSRIQFNTGLPKTFFLFKPPKGVQVFEMP"
     gene            complement(36273..36887)
                     /locus_tag="PLG98_00145"
     CDS             complement(36273..36887)
                     /locus_tag="PLG98_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE31685.1"
                     /translation="MDIDIREIYDRFAQTYDKNRYLFDMTDVMDDFFRRLPSIKGSLL
                     DLGCGAGEPFPAYFIQKEWQVTGVDFSSKMLELAHRYQPAMKTIAADIREVEFPGEQF
                     DAVTTIYCLFHIEYEKHEGIFRKIYRWLKPGGRGLFTYATKAYTGADSFSGYRKFMGE
                     RLFYSHTTPEKLRGILETIGFKIDAFQYRSIGGETFLWVTIAKP"
     gene            complement(36897..37826)
                     /locus_tag="PLG98_00150"
     CDS             complement(36897..37826)
                     /locus_tag="PLG98_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE31686.1"
                     /translation="MNLEEKIFLLQEIFKDCILCPRNCHVNRTKNERGYCGLAASIIM
                     DCALAHHGEEPPLSGTHGAGTIFLSSCNLGCIYCQNHQISHHITGQEMNSRQLADVML
                     DLEKRQCHNVEPVTPTSQMPLVMEALLIARKAGLKIPFVYNCGGYESPDILKRLDGMV
                     DIYLPDFKYGQEEDSMMFSAAPGYPWFAADSIKEMVRQVGDALELKNGIARKGIIIRH
                     LVLPGRIENSKAVLRLISRKISNNVFLSLMSQYTPMAKVRNHPDLKRRITKKEYEQVL
                     DYAEKLGFHNLFIQEVSDFDLTPDFSLDNPFKS"
     gene            complement(37858..38925)
                     /locus_tag="PLG98_00155"
     CDS             complement(37858..38925)
                     /locus_tag="PLG98_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31687.1"
                     /translation="MSRIRIKAGRKIYQLIKDGGFNFDLISTYFGPAVGPRWLIASGF
                     DLTLLERCSLGRSTPVQLVGSSAGAFRFAAWLQPEAVKSYRQLMEAYIHVTYTKKDTP
                     ATTLEKITGIINAYLENDALPFALNNKKYRLTIITARSRGLVAFQNVLLQKTGLIFCF
                     LLNYLSRENIYKFSERVVFYNASKPPAFCLKPQFRGIYVPLNEINFKYAVLASGAIPL
                     VIEGVRDIFGAPRGVYRDGGLIDYHLTHPFASGENDIVLFFHHQERIIPGWLDKKITR
                     RAPDPNILNNVLMVFPSQYFTESLPGGKVPDRTDLVTFIDDPQTRIRNWCKAVDLSAP
                     LGDDFLELVESGRLRDVVEPF"
     gene            39402..40658
                     /locus_tag="PLG98_00160"
     CDS             39402..40658
                     /locus_tag="PLG98_00160"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOE31688.1"
                     /translation="MDIKDSVEHIARNAALAAKKLSRVTETEKNTALLRMAEALERNS
                     KFLLDENAKDVNGAKKAGISKAMLERLTLKQSTIDQMAKGLREVATLPDPVGKIISMW
                     RRPNGLLVGKMRIPLGVIGIIYESRPNVTVDASALCLKAGNAVILRGGSESIKSNLAI
                     ASVLRKVLQDTSIPVEAIQVIPVTDREAVAAMLKMEEYIDLIIPRGGEDLIRAVVAQS
                     RIPVIKHYKGVCHIFVDASADMAMAVDICLNAKTQRPGVCNAMETLLVHQTIAGKFLP
                     VVANKMKKAGVVIRGCEKTKSILKRIEKAVEDDWSAEYLDLVLAVKVVENMNEAIAHI
                     EKYGSLHTESIITGDYSNAQRFLNDVNSSTVLVNASTRFSDGFELGLGAEIGISTTKL
                     HAFGPMGLEELTTTKFIIYGNGQVRT"
     gene            40655..41314
                     /gene="nadD"
                     /locus_tag="PLG98_00165"
     CDS             40655..41314
                     /gene="nadD"
                     /locus_tag="PLG98_00165"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOE31689.1"
                     /translation="MRWGLFGGTFDPIHFGHLRGAEEMLEIFNLNRIIFVPSSRPPHK
                     LEADITAFNHREQMIRLAIEDNVNFSFSEVENLRAGKSYSVETVEYILNKYMEDLELY
                     FIVGQDAFQAVTTWKDWERLLLLCNFAVMTRPGYDDMRLDAILPRTFAAKFIYDKKTD
                     GFKGPTGQAIYFRHTSFLDISSSRMRDMVRTGNSIRYLTPGKVRQYIMKHNLYKECRD
                     N"
     gene            41344..42144
                     /locus_tag="PLG98_00170"
     CDS             41344..42144
                     /locus_tag="PLG98_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE31690.1"
                     /translation="MNYKFIELEVKDHIATVTLAREKSLNAVSLELASEITDVFRKLS
                     AMDDVRVVILKSRARIFCAGLDLKDAAGGFTGTTRAPWDMIKKSQPLFDCCNVIEECP
                     KPVIAAVHGQCIGAGLHIISACDIRLCTQDATFCLKEPKIGLCADMGALQRLPLIVGQ
                     GYTREIAFTVAFYNAQQVEKMGLVNHVYADQAALYEAAREMARQITENAPLAIECTKE
                     VLNFSRGTSVSEGMSLAIQKNMFLLATDDLKEAFVAFMEKRQPDFKGR"
     gene            complement(42236..44245)
                     /locus_tag="PLG98_00175"
     CDS             complement(42236..44245)
                     /locus_tag="PLG98_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOE31691.1"
                     /translation="MAYRTDTLCGIFHNQALRYGDQVELLRAKFDQNGNPSAQWHSRT
                     WKETREEAIGLAKGLMVLGLKKSDRIVIFSESRPRWIIADQAIQACCAIGVPLYPTLT
                     VEELGYMTSDSGSKIVIASTQGRGEMALKAKAQGVPIEKIIVMCSWEGAKPEGVYTFD
                     EVIDLGKGKISDEEIEKNINSVTPEDVTSIIYTSGTTGKQKGAILTQKNWVSNMLQAS
                     NSTLMRKQRELGLHLTFLVHLPMCHVFGRTSDYHVGALQQSGILVFAESFEKIPKNIL
                     EVRPNVLISIPRLWEKIYDTTRSIVSRQDKRVQSIFNWATRLGEKYCEAMATATRLGF
                     LDLLQFALANILVFNKIKKTAGLDHVVLGISGGGKLSKEVCVFIRALGIQLAEGYGLT
                     ETSPVINFNEPEFATSFDISKASWFQKKMLDLTVDLMVTKQAKGLSPYTNPLRSLSLS
                     LAYNTLAHKLQIKPGTVGRPALWTEEKIAEDGEILVKGPQVFQGYWNLPDATKEAFTD
                     DGWFKTGDIGEFDKDGFLTITDRKKELFVTSGGKNIAPHPIELALTAKPYIDQVCLIG
                     DARKYISALVVPDFQELHRFSKHNGIPACSDKDLLANEQVKELIQKQVDEVNEALPRY
                     EQIKYYKLLDKPFSVETGELTPTLKLKRRIVFEKFKDDIESMYKD"
     gene            complement(44374..45363)
                     /locus_tag="PLG98_00180"
     CDS             complement(44374..45363)
                     /locus_tag="PLG98_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE31692.1"
                     /translation="MNHKNVYLKDIKQGDKVASSFLVTEKNMAFSQKGSPYLTVRLKD
                     KTGEMECKVWENALEFDNVFKKGDVIFIEGRAASYKNSLQISILNIKNIPRQDIDPAD
                     YLPAVKTDVKTMFAELLAFAEKVNHRHLKALLNAFLQEEETAARFRRAPAAKGFHHTY
                     LGGLLEHTLSVVRLLDKVADHYSILDRDMLITGGILHDIGKIYEFSFESIIDYSDEGR
                     LIGHIVMGVEMIDQKIAAINGFPPQLAMEMRHIILSHHGEFEFGSPKRPKTLEALVVH
                     YIDDLDAKFNAFQTFMNESSSPDSDWTVYHRFLERFLYKKKNAAFDIPEDPKI"
     gene            complement(45360..46133)
                     /gene="fdhD"
                     /locus_tag="PLG98_00185"
     CDS             complement(45360..46133)
                     /gene="fdhD"
                     /locus_tag="PLG98_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOE31693.1"
                     /translation="MNDLQKIIKTIRCFSGSYSAVSGSRVKVIQEVMITVRVNKTPLA
                     NIACAGIHLEELAAGFLRSEKIITRRQDIENISLAKNRVDVLLKNKNFFPDVSVKNIA
                     SSGARGRSNVALLTPLNVPAGLSINTALALKLMDDLLNGSAMHNETGGTHCSALAFRK
                     KIAALREDIGRHNTIDMLGGYALLNKLNLHRAIILTTGRISSEIVYKIWNMGVPFIIS
                     HSAPTANAVTLAKQANITLIGYVRKGRMSIYSHERRVII"
     gene            complement(46130..48043)
                     /gene="selB"
                     /locus_tag="PLG98_00190"
     CDS             complement(46130..48043)
                     /gene="selB"
                     /locus_tag="PLG98_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOE31694.1"
                     /translation="MKHFVLGTAGHVDHGKTALIKALTGIDTDRLKEEKQRGITIELG
                     FASLKLPSGLTLGIVDVPGHEKFVKHMVSGAAGIDLVLMVIAADEGIMPQTREHLHIC
                     SLLGISRGIVALTKIDLVEKDWLNLVESEITDFLRGTFLESAPVIPVSAIKQEGLSEL
                     LQALDTIIAEITARTDDGIFRLPVDRIFTMKGFGTIVTGTLVSDHLKTGEDVQILPQN
                     ISARIRGIQVHNRPEEEAWAGQRTAVNLQGIEKSAIVRGNVLVRPHTVWPTQRLDIAV
                     DYLSANTKSLKNRALVRLHTGTNEIISRIILLDTDELAPGQQGYAQLILSEKDVVVAG
                     DHFVLRSYSPVTTIGGGVIIDPLPQKHKRFNRGAIEELMLLQKGTLPEKITVILDRTG
                     FKGTPLQQLAFRLGMKSRKIREVLDDLFSKKEAFLLDSEDTTVISARFFNRLEEMIIY
                     NLSGYHKKNPLKEGIPKEELKGSLANDISPKLFNMVLNTLNKKETIVSNKDNVRLAKH
                     QVELAGDLDALRQNIIGIYKTAGLMPPSLTDVINKFIDRKNKAQNIVSLMLKEGELIK
                     INEELCFSGEILSKLRNDYKAMLIKDGKATPASFKELTGLSRKYIIPLMEYFDMNKLT
                     VRVGDHRVLREKT"
     gene            48210..48641
                     /gene="ruvX"
                     /locus_tag="PLG98_00195"
     CDS             48210..48641
                     /gene="ruvX"
                     /locus_tag="PLG98_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOE31695.1"
                     /translation="MRILGLDYGEKRIGAAICDELGLTAQGLSTIIRKTKKHDGEILE
                     RLIRDFNVEKIVIGYPLKLDGSEGIQCEKVNRFAALLEKTFGLPVIRWPETLSTKEAE
                     EILINSGIRWGKRKKMVDKLAACLILQSYLDSVAPSLKNNF"
     gene            48648..49829
                     /gene="mltG"
                     /locus_tag="PLG98_00200"
     CDS             48648..49829
                     /gene="mltG"
                     /locus_tag="PLG98_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOE31696.1"
                     /translation="MKNPDKKNILKKSNEENNAVDQMTSQASAWKSKFHEIFIFLKKI
                     FSGRRWIRRTVLYSVAVILIFCIMLLDYAFSPIDVRDKTVVIDIPTGSSFMEATELLQ
                     KEGLITNRFFFYSLAAIKRASRNIRAGEYEINTLTNPITMINKLMHGDIKVYEVVIPE
                     DLSFRDIAVILDKHKLINKEIFFELARDQEFLKSLNIEAESIEGYLFPDTYHFNRSMN
                     TRRIMKKMVDTFWQKMTPSMIRRANQMGLSVHKLVTMASIIGKESGSALEKPSVAAVF
                     YNRLKRGMPLQSDPTAVYDMDDFEGKIFRSHLKRNSPYNTYRIKGLPPGPIANPGLDS
                     LKATLFPSSVDYLYFVSKKDGTHHFSSSLVEHNQAVNRYINLKDEQNQLLEYDIPVEE
                     Y"
     gene            50034..50480
                     /gene="mraZ"
                     /locus_tag="PLG98_00205"
     CDS             50034..50480
                     /gene="mraZ"
                     /locus_tag="PLG98_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOE31697.1"
                     /translation="MSVFRGQYHHTIDEKGRIIFPSKFREIFAEEYDNRMVITNWDQY
                     LMVFPYDEWLVIEEKVAHQSKLRKEVRSFQRFFMSGAIDCSIDGQGRVLIPPSLREYA
                     QLEKDIVLAGMLKNIEIWSKDRFEERMKQAVGEIDKCADFIADLGI"
     gene            50484..51419
                     /gene="rsmH"
                     /locus_tag="PLG98_00210"
     CDS             50484..51419
                     /gene="rsmH"
                     /locus_tag="PLG98_00210"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOE31698.1"
                     /translation="MTAEFYHQPVMVKEVIDSLIVSRAGVYVDGTVGGAGHSYAILKQ
                     TDALLIGIDCDDEALRFAEKRLQEFGSRKILLKENFVNLDHILKSLNIEKVDGVLLDL
                     GVSSRQLDASERGFSFSQQAPLDMRMDRSMTLSAYDIVNSFAQNELEKIIRFYGEEKM
                     ASRIARAISKKRQESPIATTTQLAAIVASCMPAGTRWRKIHPATRTFQAIRIAVNHEL
                     DNIKPAMHAAVDVLKTGGRLCMISFHSLEDRIVKNEIRSLEGRCVCPPDSPFCVCQRD
                     AKLKNLTKKAIMPSEEEIASNPRARSAKLRVAGRL"
     gene            51421..51768
                     /locus_tag="PLG98_00215"
     CDS             51421..51768
                     /locus_tag="PLG98_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOE31699.1"
                     /translation="MAPSAQSADARVVPHLGEVEGASVGIKYPTFIVLAVVLMLVFVA
                     YVGSHIRMTELEYNIAAEISTQEKILEEQKKLKLEYAMLKSPQRIENLVRDRLQMSYP
                     DNEQVIVIRKSGG"
     gene            51770..53746
                     /locus_tag="PLG98_00220"
     CDS             51770..53746
                     /locus_tag="PLG98_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOE31700.1"
                     /translation="MAIESKKRMKFRIIMIFMFFFVLFVALISRAFQLQVLSGQKLKK
                     LATRQHTTVLQLQPERGIIYDRNGEKLAISIMADSVCADPSKVIDPAKASKQIAYILG
                     LDSQAVYKKLSAPRNFCWLARRISPEQTAAVVKADVEGVFLVKEPKRFYPNGELAAHL
                     LGFVGLDASGLEGLEKKYNDSLVGSPEKLEWARDAKGKKLFLRVEKTQTAKNENTNLV
                     LTIDNRIQYLVETHLKEAVIEKGAKGGLAIVMDPKTGEILALANQIGFNPNNIQGLSP
                     ERWRNRAITDYYDPGSTFKPFLVAAALEEKVIREQDRFFCENGNYRIADRVIHEANRK
                     RYGTLTVREILKYSSNIGSAKIAQKMGKEKFYNYIQKFGFGSKTGIDLPGESSGLVRP
                     VRDWVKVDTSMIAFGQGISVTAIQLISAVSAIANNGVLMKPYIVRGIADQDNRLVKLY
                     TPTVVRQVISPRVAKSVTNMLMDVVGAEDGTGKNAKIVNVAVAGKTGTSQKFDTARGV
                     YSSEKVRTSFMGFFPAKDPQVAILVVLDEPQKDKWGGVASAPVFKNIAEQILNCFKTD
                     IRETPVFKKEKTDKVQLVSAEQTLLPEIVENSDGPSMPNFMGLTIREALKKAKTESIE
                     LKISGNGWAVRQYPEPHAPLGHERVCSVVFELTD"
     gene            53754..55277
                     /locus_tag="PLG98_00225"
     CDS             53754..55277
                     /locus_tag="PLG98_00225"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOE31701.1"
                     /translation="MKLTRLTEGLDMINMSGEASGDVSGVCFSADQCVPESLFVAIPG
                     LKRDGHDFIEEAISRGARFIVHEKVLTFPPFVRAIKVSDSRRALGILAKNYFGDPSAA
                     LTVIAVMGTNGKTTISYLLESILKGAGCKCGVLGTVNYRYNDKVYDAPNTTPESYEMQ
                     KILRAMADDGVTHVVAEVSSHAVDLKRVDDCDFDLGIFTNLTRDHLDYHVTMENYFQA
                     KKRFFAEILPQSRKGYAQKMIINADDDWGRRILKDVPLPALTYGIENDCAVRSLNCKL
                     SLNGIEADIRLAGNNVLIQSPLIGKFNLYNILAAAGAAFILHIPVPVIKAGIENLSHV
                     PGRLESVPSAGGVHVFVDYAHTDDALRRVLQNLSELKKNKIITVFGCGGNRDKGKRAL
                     MGDAATRYSDLTIITSDNPRLENPLDIIADIEAGIDRKDVKKSDPEMLKMNGRARSYA
                     VIPERRKAIEAAIRAARRDDIVLIAGKGHEDYQILGTEKISFDDRVVARQILETKSY"
     gene            55286..56734
                     /gene="murF"
                     /locus_tag="PLG98_00230"
     CDS             55286..56734
                     /gene="murF"
                     /locus_tag="PLG98_00230"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOE31702.1"
                     /translation="MKSESPVFFLNDVLTSTSGTMMSGLPETVFRGVSTDSRSIKKGN
                     LFVALKGDHFDGHDYAESVFQGGAAGILAQDDEKIRRMKVDDTFAVIKVADTLFALGE
                     MAHDWRKKFSIPVIGLTGSSGKTTAKEMIASILGLKKNILKTEGNLNNLIGLPQTIFR
                     LTDQHDMMVLEMGTNTRGEIRRLTRIAEPDIGLITNVGPAHLEGFGSLQVVGEEKGDL
                     FLNMPSSGIIVVNLDDEAILTAAETWKGRKITFSMSPNADVTVFDIERNGAKGVRFNL
                     AIRGNTQKVEMKITGLHHINNAMAAAAVAVAAGMDLKNIAEGLSAFRPFHGRMEIIKL
                     RNGAYLLDDSYNANPASVREALMTLKDLKGNHHAYVFLGDMLELGAQADEMHRKIGML
                     IATIGVKTLFLQGDYAGMTAAGATEGGMASDSILFLSDKENGTDFLKKCLKKGDWILV
                     KGSRRMNMDRIAAQICEDFGRDKVSEENKTDY"
     gene            56751..57830
                     /gene="mraY"
                     /locus_tag="PLG98_00235"
     CDS             56751..57830
                     /gene="mraY"
                     /locus_tag="PLG98_00235"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOE31703.1"
                     /translation="MIYHLLYPLHTTISFFNVFRYITFRTIFAAITALLICLIFGKWM
                     IRKLQDMQIGQQIRDDGPQSHLVKKGTPTMGGMLIIMAVVVSTLLWTNLADVYVWLIL
                     MVTLGFGLIGFIDDYSKLAGKSSRGVSGKTRLAAEIIIALFVSVILYVKPGFNAQVTI
                     PFFKTVLPDLGWGYVLLSTFIIVGAANAVNLTDGLDGLAIGPVVICFATYILFAYFAG
                     NIKTSNYLQIVHVSGAGELAVFAGAIVGAGLGFLWFNAYPAEVFMGDVGSLSLGGALG
                     TIAVITKQEILLAIVGGIFVVETFSVIFQVGYFKMTGGKRILKMAPLHHHFELKGWAE
                     PKVIVRFWIISILLALMAISTLKLR"
     gene            57838..59184
                     /gene="murD"
                     /locus_tag="PLG98_00240"
     CDS             57838..59184
                     /gene="murD"
                     /locus_tag="PLG98_00240"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOE31704.1"
                     /translation="MDFNGKKIVVIGVGKTGVAVARFLGKKSARIVVTDEKSPDHWDG
                     GFKQIAGEPWLEVGEYNSVDVLNDADMVIPSPGIPPGNPLLAEALKKNIAVVSEIELA
                     YRFIRVPIIAVTGTNGKTTTTTLLGEILQKSGKKVFVGGNIGNPLIEYADKNQDADFI
                     VAEISSFQLQWIDQFRPFIAVLLNITSDHVNYHGSFAEYRRIKARIFANQTGKDFAIL
                     NAEDDEQKKIERNIRARVAGFSSKHVLPSGIFVKEENIVLSLPGMQEEKYPLSMINIP
                     GLHNVENVMAAILAARFCGCDPSDTIRAVENFRGLPHRIEFVEEKNSVKFFDDSKGTN
                     VGSVLRALETFTQPVILLLGGRDKDGDFETLQPVLAAKAKKIVLFGEARERIASLIGQ
                     TVPVIKKTKLQEAVEVAYQEAVPGDVVLLSPGCASFDEFANYKERGNFFRKVVENL"
     gene            59185..60294
                     /gene="ftsW"
                     /locus_tag="PLG98_00245"
     CDS             59185..60294
                     /gene="ftsW"
                     /locus_tag="PLG98_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOE31705.1"
                     /translation="METAYKKEDNYPDFVLLLVTLLLAVVGTAMIYSSSSIIALEKFN
                     DGQYFLKKQLVFVFLGFTIMVAMTKVPYTHLRKIAYPGMLLCLLLLSLLFVPGIGMRR
                     GGATRWLNLGGFSFQVTEMVKIMIVIFLSHLLTRKAFQITNFTKGVLVPLAATSVVMM
                     LILLEPDFGTTAIIATIMLMMLCIAGAQIKHLALLIGAFIPVGIALILYKGYRLTRLT
                     AFLDPWKDADNTGFQIIQSLISFGSGGTFGVGIGDGMQKLFYLPEPHTDFILSIIAEE
                     SGFIGVAMVITMFALFAYRGFRIALNAPDLFGSLLASGLTMVICMEAFINIAGVMGLI
                     PLKGLVLPFLSYGGTAFIMSMTAVGILLNISTQTS"
     gene            60349..61434
                     /gene="murG"
                     /locus_tag="PLG98_00250"
     CDS             60349..61434
                     /gene="murG"
                     /locus_tag="PLG98_00250"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOE31706.1"
                     /translation="MRIVIAGGGTGGHLFPGIAIAEEFIKRNAQTKVVFIGTKRGIES
                     RLLSRLGYDLETIDIEGIKGRGIRALVKSVFQIPKSLWQARGILKQFSPDMVVGVGGY
                     ASGPAVLAAYLMGIPTSIAEQNAIPGVTNKILGNVAGKIFVTYPRTQQCFPRRKVVIS
                     GNPVRASFAAQSFRTKEDRTHRQLLIFGGSQGAEAINRGVIDMLPRLSGMKDRIRIVH
                     QTGARQLEEVKKAYTQSGLQAVVTDFIMDMAEAYAQADLIICRAGATSLAEITVAGKA
                     AVLIPYPWAANDHQLKNALAMSDAGAAVVIPEKELSGESLFNAIDGILRDENRRLLME
                     ENSLRMGKAEAAATIVDECMKLTDAKH"
     gene            61464..62876
                     /gene="murC"
                     /locus_tag="PLG98_00255"
     CDS             61464..62876
                     /gene="murC"
                     /locus_tag="PLG98_00255"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOE31707.1"
                     /translation="MKKDKEKSLMQRKIKCIHFVGIGGIGMSGIAEVLVNLGYNVGGS
                     DVQPSETTARLQKLGAKIVIGHAAENVGNADVVVTSTAVKEHNPEVLEAHRKNIPVIP
                     RAEMLAELLKMKFSVAVSGSHGKTTTTSMISTILAEGNLDPTMVIGGKLASIGSNARL
                     GDGEIIVAEADESDGSFLKLSPTIAVITNIDREHLDYYPGIAEIKEAFLTFANIVPFY
                     GCTVLCNDNIHVREILPAIKRRMITYGIDTPADYSAEDIKFTKRKTCYSLVHKGEKLG
                     KIELIVPGLFNVYNSLAAIAVARELNLDLATIKEGLKSFSGVQRRLEIKGRVGEVTVL
                     DDYGHHPTEIKATLAAMRQIWKDRLIVVFQPHRYTRTRALFEEFTKAFDEVDILILND
                     IYPASEEPIPGITAAALCEAIRKAGHKHVEYIPGAEATVEYLLKTVHQKDTVATIGAG
                     SIYKIGETFITQLVARGKNT"
     gene            62876..63805
                     /gene="murB"
                     /locus_tag="PLG98_00260"
     CDS             62876..63805
                     /gene="murB"
                     /locus_tag="PLG98_00260"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOE31708.1"
                     /translation="MTSHCEIRGILQEIDAGKVLYDEPMSLHTSLAVGGKADALVVVE
                     HENQLARVIRKFRMEGINYFTAGNLTNVIVKDGGYRGAIILMTGMKEIQCKPVGGHEG
                     YILAQTGASLAKVVSRSVVEALSGVEFCSGIPGSVGGAVWMNAGAYGREMKDVIESVT
                     VLDTEGSKKTMIREAISFGYRTSSFPSGTIILGARFRLKKGDRATMKARISEILQWRQ
                     AKHPLEYPSAGSVFKNPPGHPAGKIIEDMGLKGEKAGGAEISSKHANFIVNKGGATAA
                     DVLALIRLVQSRAREEKNIDLETEVVVIGEDRC"
     gene            63799..64638
                     /locus_tag="PLG98_00265"
     CDS             63799..64638
                     /locus_tag="PLG98_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOE31709.1"
                     /translation="MLKKTGIQTKQNRIRRRFTSLSGDFFTAMTLLSMAVFLSVLFIY
                     VYNVMMSSPYFQIKEISVRGLKELTEKDILASANIKPQQNLLAVNKNSIIRRVCSNHW
                     VETVYVGKELPGKLVLDVRERIPLALVKRGGDFYLMDVKGHVFKRLGGSDEVDLPVIT
                     GAATGEATRSPLLLSALGLIQRISKSPAYAHLGTISEIHIDSVFGLALVSDNGLYLKL
                     GTDDFENKLRKLKAILTDLENRGMKTGFLCVDLSDHSKVTVKRKYVPEKTQDGDQNKN
                     YLI"
     gene            64671..65888
                     /gene="ftsA"
                     /locus_tag="PLG98_00270"
     CDS             64671..65888
                     /gene="ftsA"
                     /locus_tag="PLG98_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOE31710.1"
                     /translation="MGKRSNVLVGLDIGTTKTTAIVGDVTETGIDIIGIGTSQTKEMR
                     KGTVVNIDTMVESIKTAVEEAEHMSGCRINSAFVGISGSHIKGQNSLSIVSIKGREVD
                     EKDVQRVIEASEAIAIPVGRKILHILPQSYVIDGQEGIKDPIGMSGVRLEAKVHIVTG
                     AIAAIQDIEKSVNRVGLDIDGIVLEQLAAGEAVLSSDEKELGVVLVDIGGSNTSVTIF
                     SESSIKHTAILPVGGNYLTSDISTGLRTPLSEAEKIKIKHGCAMTSMIPKDETIRVPS
                     LGGREDREVSRQILGRIVEPRMEEILNLAYNEVVKFGFEDLLAAGVVLTGGTSLLEGI
                     SELAEKIFDMPARKGIPIGVGGLNDIVNSPAHAIGVGLIIYGHKQNALSGVSTGMFGH
                     ITRTVKKMFSEFF"
     gene            65904..67061
                     /gene="ftsZ"
                     /locus_tag="PLG98_00275"
     CDS             65904..67061
                     /gene="ftsZ"
                     /locus_tag="PLG98_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOE31711.1"
                     /translation="MFELTEVCSENLAKIKVIGVGGGGGNAVNTMIASCLQGVDFINA
                     NTDAQALGVSSAPVKIQIGSQITKGLGAGSDPEIGKQSALESRDEIRPYLEGADMIFI
                     TAGMGGGTGTGAAPVIAEIARECSILTIAVVTKPFQFEGKKRNMQAEEGIEQLREKVD
                     TLIVVPNQRLLSLGGRNLSLLEAFKKADDILYQAVKGISDLILVPGLINLDFADVRSV
                     MSNMGMAIMGTGIASGENRAVEAAQRAISSPLLEDNTIQGAHGILLNITGGSDMSLYE
                     VNEASSLIQEEADEDANIIFGTVIDQNMEDEIRITVIATGFDDFARKKNDSVGNVTRF
                     RGVGGREDLATPTFIRRERKLNSGEIAVVGLGDETENMDIQIPTFLRRQAD"
     gene            67128..68816
                     /locus_tag="PLG98_00280"
     CDS             67128..68816
                     /locus_tag="PLG98_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE31712.1"
                     /translation="MDWKLKAVYKSILENETGYVKKIWGKDNTVCLVYPNHYRIGMAN
                     LGFQTVYQLLNAQPSFLCERAFLSVSGNEARCVSGTAGIFSLENQKALTEFDILAFSI
                     SFENDYPNILKIMDDAGMPLRSADRSEKYPLILGGGIAPTLNPEPLADFFDLFVLGEA
                     EDILPVFCRFFEASRRLGQRREQFLKDAQKQLHHVYVPGLYDVHYSPERKITSVVPRE
                     AGLPEKIKVEPVNDINAFRTQEVISASCTEMEDMFLVEVNRGCAHGCRFCAASFIYRP
                     VRFRGIEEMIGAIHDGLTRKNKIGLVGTAVSDHPDIPAVCRYIIAHNGRAGLGSLRID
                     RINEEMVGLIKANGIETVALAPEAGSQKMRDILKKKISEEQIMYATALLAEHGIANIR
                     LYFMIGLPREEESDIDAIIGLTKKIQHHVRLRFDGKKQFRRLTLSINQFIPKPATPFQ
                     WVPLLDVHIAGKRIKKIKEAFRKDKSVRVIHDVPKWNYVQALLALGDRRVGEILLAVN
                     RLDGNWGRGLKEVNVNPDFYVYREKSFDEILPWDIIDLGISRKALINENQKALK"
     gene            complement(68827..70098)
                     /locus_tag="PLG98_00285"
     CDS             complement(68827..70098)
                     /locus_tag="PLG98_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HOE31713.1"
                     /translation="MDGETALIFYYLKGNLNSYNALAGALEKYPDIKNIDVYFLSSEK
                     ELLQKISAISKKHRETIIGISFCTPQFLKIKDLINRVKKLAGETVFLAGGPHPAGAPL
                     ETLKLGFDFVISGEGEETLTELLSKINHDENNSDIKGLSYLDKNGEYVFTGRREAADI
                     NTFYPISHYFSKYGPIEITRGCPYACRYCQVSQLFGTTPRHRSIENILDCVKIMKRQD
                     STYFRFISPNAFSYGSEDGKRINNEKLENLLSDIRRLVGKSGKIFFGSFPSEVRPEHV
                     NAHTVKMVKKYANNDNLIIGAQSGSQRMLDCAGREYDVATIYNAVDVTRQAGLKAKVD
                     FIFGLPGETDEDIASTLNVIQDLSRSGAKIHAHTFMPLPRTAFENETAGKIGPALKKE
                     LNRLCANGIIYGQWQQQEKIAAGIAAKQDNA"
     gene            complement(70343..70588)
                     /locus_tag="PLG98_00290"
     CDS             complement(70343..70588)
                     /locus_tag="PLG98_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOE31714.1"
                     /translation="MADSKSDFFTGLIVGGLIGVALGILFAPKSGKESREDIARKADE
                     LMAKAKEGYEKAAGKYSEMTGVESFRDYKTADNEDLA"
     gene            complement(71017..72405)
                     /locus_tag="PLG98_00295"
     CDS             complement(71017..72405)
                     /locus_tag="PLG98_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOE31715.1"
                     /translation="MTNAKRAEVHAIAMETVKAAEKVPEKYFVVDTSALEYGMDVFEK
                     LRQGGRNCVLIPYATWEELDSHKTGNDFKADIARNVIRNVRNLFRAKDHSVRFFQYDF
                     NLVKDADLKVDKNDHCIIATALNAKKKLGKAKVILVTQDAALEALALNFGLDVQSIKM
                     HQTDIKQHHTTRATINLQRSWISDKTFPLKLPEAKLAAQLEQVLYNEGIICYCDLDGV
                     WGPAFVAVRKGDYFRIIDRDIHLLGIKPRSNNGDVNWGQFIAMDLLRDATVPLVTLTG
                     KAGTAKTFLALLAAFEQASHYRQILVSRATIQLGNKDRLGYSPGDIDAKMKPWMHPIY
                     DNINAIKELVDERMKEKINDWLANGKIEMMDLDKIRGRSIQKCYVIVDEIQNLPPSHV
                     KAIATRGAEGTKMVFTGDFSYDQIDVPWLDERSNGLSVLNAKMAGSRFFGSVHLDQAI
                     RSELTKEILERW"
     gene            complement(72680..74419)
                     /locus_tag="PLG98_00300"
     CDS             complement(72680..74419)
                     /locus_tag="PLG98_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE31716.1"
                     /translation="MKKNLFYKIFFSYLVIICLSFFLLDIFVRDEVRKVLTSQIETEL
                     LSYAQLIDQTSLEKASGHLKQVAQISQSRITFMDARGRVFADSEKDIALLESHFNRPE
                     VQEARLRGTGKSVRYSDSLGIDMLYMAVAVKDAQKIKGYVRLARPLHHVQNVIDKVYQ
                     YILLTLFVVALIALGIALFFSYRLYEPIKIMEQFTEKLRRGDPVGSIILDTSDETKTL
                     ADNINYLVEELREKINIANEEKNKLMTAFTSMNEGVLILDEQGVIEFSSPVLSNMLAI
                     QYDDVVGKTLMEAFRNIDLQKAFQEFKEKCISVSREIILGHADKVVLNVSLSAVHGRP
                     DEEKTMIVFHDVTRLKKLEQVRMDFVANVTHEIKTPLTAIIGYLETIQSGAITNQEET
                     KKFIDIILKQAERLNRLVEDLLTISNIETKQTVLNFESVSLRKAIDTMLSLIEAKAEL
                     KNISVRNNVSDNFPPITANRDRLTQIFVNILDNAVKFTPNGGEISINASESGSYTVVS
                     IQDSGIGVPKDDISRLGERFYRVDKSRSRDLGGTGLGLSIVKHLMIAHGGRMEIESQP
                     GKGTKVSLYFPVK"
     gene            complement(74431..75114)
                     /gene="phoU"
                     /locus_tag="PLG98_00305"
     CDS             complement(74431..75114)
                     /gene="phoU"
                     /locus_tag="PLG98_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOE31717.1"
                     /translation="MEERRHTSSHYEMELQDIKNNLIYLGALTEKAIHSAMTSLTDRD
                     TDLAKKVIANDYEIDKMDKDIEEKCIKMLALRQPTAIDLRFITTAIKITGHLERIGDM
                     AVNIAEKAIQLNEEPKLKPYIDLPNMSSIVEKMVAKALDALIKRDLQIADDVRQIEQE
                     VDDLNEQIFRELLTFMMEDSKSIHRALLIMHVSKNLERIADHAQGIADMVTYMITGEN
                     VRHLSRDEK"
     gene            complement(75136..75891)
                     /gene="pstB"
                     /locus_tag="PLG98_00310"
     CDS             complement(75136..75891)
                     /gene="pstB"
                     /locus_tag="PLG98_00310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOE31718.1"
                     /translation="MDSRIIQLNNVNFYYGESRALTDISMTFVKNKVTALIGPSGCGK
                     STLLRLLNRMNDLIDGTKVEGEILFESKNIYAPDVDPVEIRRKIGMVFQKPNPFPKTI
                     YNNIAYGPKLTGIAVDDMAALVEQSLKQAVLWDEVKDILNKSAMNLSGGQQQRLCIAR
                     ALAMKPDVLLMDEPTSALDPISTAKIEELIEELKKDYTIIIVTHNMQQAARISDETAF
                     FYLGNLVEYNKTEKIFTNPDVKQTEDYITGRFG"
     gene            complement(75894..76772)
                     /gene="pstA"
                     /locus_tag="PLG98_00315"
     CDS             complement(75894..76772)
                     /gene="pstA"
                     /locus_tag="PLG98_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOE31719.1"
                     /translation="MQILQTHAMKWRYFKQGLFFSCVRLSALMITLALGGIISYILIN
                     GISVISWDFITKPPTDAMTKGGIMPAILGTIYLTLGAIAIGLPLGIASAIYLTEYARQ
                     GRVVRLIKVGINCLAGVPSVVFGLFGLGFFVIFLKFGSSILAGSLTLGFLILPTIIGA
                     SEEALKAVPQTFREASLSLGVSKWQTIYRIVLPNALPGILTGSILGIGRAAGETAPIM
                     FTAAAFFTAKLPSSIFDEVMALPYHIYVLATAGTNIEATRPIQYGTVLVLVAVVLGID
                     LIAIIIRGYMRKNKRW"
     gene            complement(76784..77671)
                     /gene="pstC"
                     /locus_tag="PLG98_00320"
     CDS             complement(76784..77671)
                     /gene="pstC"
                     /locus_tag="PLG98_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOE31720.1"
                     /translation="MTRQIKEIIIKYIFFIFSLVSVLVLGLIVFSLFREGIPLFGKIS
                     IKDFLFGMEWYPTYDPPSFGIFPLIVGSLIVTILSTFIAVPLGVLAAIYISEIAPQNI
                     KITLKSVIELLAGLPSVVLGFFGMVIVAPWMQETFDLPTGLNIVNASLILALMAIPTI
                     SSISEDALYAVPREFKEASYALGATKFETIARVILPAALSGISTAVMLGMARAIGETM
                     VVLMVAGGAAAIPESLFDSVRPMPASIAAEMGETPFRSLHYQALFAIGIVLFCLTLAF
                     NLIADYVSHKFKQVGSGTL"
     gene            complement(77678..78112)
                     /locus_tag="PLG98_00325"
     CDS             complement(77678..78112)
                     /locus_tag="PLG98_00325"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOE31721.1"
                     /translation="MQNDSMKKNVLFLCTHNAVRSQIAEAFLNHLYGDRYSAFSAGSD
                     PTQIDPLVVTVMKETGMDLSANQSKSLSVFPDMHFDCVITVCDQVQESCPHFPRGDRH
                     IHKRFSDPSKFNGNAEDITHEYRRVRDEIKAWVEKEFHQAIK"
     gene            complement(78153..78974)
                     /locus_tag="PLG98_00330"
     CDS             complement(78153..78974)
                     /locus_tag="PLG98_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE31722.1"
                     /translation="MFSILKKAALTIAAVALMSSVALAGSSIVIKGSTTVLPITQSTL
                     EAFMKKHPDVQMSLSGGGSGEGIKALIDKTAHIATSSREIKKEEIALAKTKNVNPVAH
                     VVAHDAIVPVVHPRNKVKNLSVDQLSQIYQGKITNWKEVGGDDLKIVVISRDSSSGTF
                     ESWDHFVMKKTKVTPRAQMLASNGAIVTAIAKNRYAIGYLGIGYLDKSVTPLQVNGIT
                     ATIQTALSKQYPFSRELYMYTDGNATGDVAKYLAFVKSAEGQKIVQKEGFVPLTK"
     gene            complement(79087..79791)
                     /locus_tag="PLG98_00335"
     CDS             complement(79087..79791)
                     /locus_tag="PLG98_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE31723.1"
                     /translation="MTMYKILIVDDEKDIVDLVSYNLEKEGFSTVKALDGESALQIIR
                     AQKPDLIILDLMLPKMNGLDVCKTVRRNPETSGLPIIMLTAKSDEVDKVTGLEVGADD
                     YVTKPFSVKELTARVRSVLRRLKENELYAGKEKFSHAGLKINYESCLVLVNGKKVTLS
                     PTELKLLFFLSRHPGRVYSRNQIIDHVWGDDTFITDRAVDVHIRRLRSQIEQDIENPK
                     YILTVRGFGYKFADLK"
     gene            complement(79884..81056)
                     /locus_tag="PLG98_00340"
     CDS             complement(79884..81056)
                     /locus_tag="PLG98_00340"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HOE31724.1"
                     /translation="MKFEHCIRCTICVENCPVFRVNPEFPGPKQAGPDAQRFRSERDK
                     AQDGWVLLCSQCKRCEMACPCGVEPAQIILKAQQRYGNEHPQTPAHLLFANNYYLSAL
                     GSFTAPLANVVASTQTGKKIFQKMGISTYLPFPKFSFRTMRREKKRLNKGRKKVAFFY
                     GCFINFYRPDIGRKIIRLLASMNVDVVLPPQWCCGLPALGNGNLTLARYFAKKNAESL
                     SNYIDAGYDVIYTCTSCGLCLLHDYPGILEIPQGRKIAENSYDVHEYIIKLINEGYVK
                     PDFREVRRSIAYHIPCHLRALKIGYPAAKLMSLIPGLEYEILDDACCGLSGSYGFKKE
                     NALTAIQIGNRAVSLISKTGMDNIVSDCGSCRMQLAGLSGMPALDPAEIIFDSLGR"
     gene            complement(81046..82254)
                     /locus_tag="PLG98_00345"
     CDS             complement(81046..82254)
                     /locus_tag="PLG98_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit B"
                     /protein_id="HOE31725.1"
                     /translation="MSVTEYDAVIIGAGVAGLTAGTLLAERGLKVALVTKGEPTACLS
                     TGCVDVCSLSKNPLSGIRSLPPEHPYNLVEEKTVKQSLEKFLADMKDAGLPYSGSADA
                     NRLILSALGTSKISCLIPSTMTAAPQENHDGSVHIITFKGLKDFYPGYIVSRRRSTTF
                     SVYDAGVSTTMSIAARFEEKDFFERFIAWMTQRGIHEDGIAFPAVLGMESAADIVDTI
                     SERTGKPVFEIPTIPPSMPGRRLFNALKKTFRNKGGTIYWSWPVAGMEKTGNIVEAVT
                     TPSEGRPNSINARAFILCTGSFVSGGLTATREGILENIFNLPVYVPGPRDTWFDNDYF
                     SLNHNIGKAGIRADASMRPLDAPWKNIFVCGSILADTQILKNGCGHGLALATAHVAAQ
                     SCAEYLLDEI"
     gene            complement(82251..83753)
                     /locus_tag="PLG98_00350"
     CDS             complement(82251..83753)
                     /locus_tag="PLG98_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE31726.1"
                     /translation="MIKTDVIIIGGGATGCGIARDLALRGIPHCLVEKGDFAAGATGA
                     CHGLLHSGGRYAVTDPVAARECYEENLILRRIGRKCVEQTGGLFVRLPGDSKRFRELF
                     LRGCEDVGIPAEVISSTLAIDLVPNLNPDIEEAIRVPDCSIDPFRLCLLNIRTSELRG
                     GMIFTRHKVTEIIKSHGRCTGVKAVDQTTGEEKEIHAKIIVNATGAWADIVATLAGSE
                     VPLTLVKGSLVITNHRLTNMVINRLRQPSDGDIIVPNEAVCLAGTTSLAIENPDNLLI
                     EASEVDTIISEAGRTIPHFNKTRIIRAFSGVRPLLKSKKADSHEITRGFQIIDHQNGL
                     LSIVGGKLSTFRLMAEKTVDTIMTIFHLKKACETAEIPLDGQEELSGYPLSKRLADMT
                     NIVCECELVTRQNVEKIIQQTATRNVGDISHRTRLGMGPCQGGFCTFRALGIMNDMSI
                     ISPEQSMEMLRGFLQRRFKGIRYALWGDQLREEQLVEYIYLGILSMEKPQ"
     gene            complement(83815..85314)
                     /gene="glpK"
                     /locus_tag="PLG98_00355"
     CDS             complement(83815..85314)
                     /gene="glpK"
                     /locus_tag="PLG98_00355"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOE31727.1"
                     /translation="MKKSYILSLDQGTTSSRAILYNHKGNIVGTAQKEFTQIYPKAGW
                     VEHDPMEIWGSQSGVVSELLATTAIAAEEIAAIGITNQRETTVVWEKRTGKPVYNAIV
                     WQCRRTADICDELKARGLEKYIRENTGLVLDAYFSGTKVKWILDHVEGARQKAQDGEL
                     LFGTVDTWLIWNLTRGKVHVTDYSNASRTMLFNIKKLKWDQKILKELDIPASMLPDVK
                     PSSMVYGHTDSRLFGAEIAIAGDAGDQQAALFGQACYTPGMVKNTYGTGCFMLMLTGE
                     KLVHSQHGLLTTIAWGINNKVEYALEGSIFVAGAAIQWLRDGMKLIYDAKDSEYFATR
                     VEDSLGVYVVPAFVGLGAPYWDMYARGAILGLTRGTTRNHIIRATLESIAYQTRDVLE
                     LMRNECGLDLCELRVDGGACANNFLMQFQSDILGVPVERPEIIETTAMGAAYLAGLAT
                     GFWKDQSTITRRRNVDRRFNPAMDDDKRHTLYNNWKKAVKRAMKWEEEE"
BASE COUNT        21973 a        19919 c        21696 g        22255 t
ORIGIN      
        1 accgcctggg tgccaacgag gcgccgccgg ccatcatctc cgtgtttctg ggggaccaac
       61 tgatccaaat tctggagaac atacaacgag gaaccgcttc gaaagcgacc gacgcgcaaa
      121 tcatcgatct gggcatttct tccctgtatc aggtcagcaa agacaatacg gaccgcaaca
      181 ggacatcgcc ttttgccttt accggcaaca aatttgaatt cagggctgtg ggctcgtcgc
      241 aatctatttc cacccagatc attgctctga acacgatcgt tgctgaaagc attgacgata
      301 ttgcggaaaa aatcatagcg agaaaagatt ccaaaaacat ctatcaggcc gtcatggatg
      361 ttttgaagga cgaaatcacc acgatcaagc cggtcctgtt taacggtgac aactacagca
      421 aggagtggga agccgaagcg gcccggaggg gtctgccgaa cgaaaagaca acccccaccg
      481 ccctgaaggc cctgattacg aagaaagcac tgaatctttt tgaaaaatac gaggtgcttt
      541 ccaacattga gctcaaatca cggcagttga tccacatcga gcgctacatc aaggatctgg
      601 agatagaagt caaatgtctc aattccatgt gtatgagcta tatcctgccg gcagccgtaa
      661 tctatcagaa acgactggcc ggagccatca atgccaccag agaagctctg ggcaccgagg
      721 ccgttgtctc ggcggaaatc gaactgctca agaaaatcag cgatctgatc aacaatatct
      781 tcgctacaaa cagggacatt cagcagaaag tggagtccgc caacgctctg catgatgagc
      841 agaagaaagc agaaatgttg agtgccaagg tcaagccaat gatggaggaa ctgcgcgagt
      901 atgcggatgc cctggagatc ctggttgatg acgatatctg gcctgttccg aaattctggg
      961 aaatgttgtt tatctgttga tatggaggcc atcatttttt cgccatgtca cgttttgccg
     1021 caaccgattt tatgcaacag ggaagttaac gatgtttcaa ctgggtaaaa aaagaaaagc
     1081 gttattggtt ctggaagacg gcagtatctt tgcgggaaat gcttttgcag ggtctggcga
     1141 aaccatgggg gaagtcgtct tcaacacggg catgaccggt tatcaggaag tgattaccga
     1201 tccttcctat aaaggccaga ttgttaccat gacgtatccc ctgatcggga actatggcat
     1261 caatgatgaa gatagggaat ccgccggcat tcatctggaa gggtttatcg tcaaggaata
     1321 tcaggctcat cccagcaact ggcgaatgac caaaagcctg aaatcgtttc tggacgatca
     1381 cggcaaaatc ggtattgaag gtattgatac ccgtgctttg accagacgtc tgcgcatggc
     1441 gggtgccatg aagggaatca tttccacgga aagcgcgaat ctggaatcgc tgctggcgac
     1501 gttaagggat tatcccggtt tggttggccg cgatctggtc cgcgaagtga cctgttcaac
     1561 cccttatcag tgggagcgca atgccgtccg ggcagacggc attgcgcccg acaaaccgca
     1621 acataaactg cgcgtggccg ttgtggattg cggcgtgaag tataatatcc tgagacttct
     1681 ggagaaaaac ggctgtgagg tcgttgtgat gcccgctttt tccaccggcg acgacatcat
     1741 gtcgctcaag ccggacgggg ttctgttttc caacggcccc ggcgatccgg cggctctttc
     1801 ttatattgtg gataccgccc gtttcatcat cggcaaggtg ccggttttcg gcatttgtct
     1861 ggggcatcag attatcggtc aggcgatcgg cggctgtacg gaaaaattaa agttcggtca
     1921 tcacggcatt aaccagccgg tgaaaaatgc cgaaacggga cgggtcgaaa tcacgtcgca
     1981 gaatcacggt tttgtggtgg ttttggaatc gatgaacgat caggctcaaa aaacgtatca
     2041 caatttgaat gacaacacct ctgaagggat gagcatgccg caggccttca gcgtccagta
     2101 tcatccggag gcggctccgg gaccgcacga cacggcatat ctgttcgggc ggtttgttga
     2161 aatgatgaaa agtgtgaaat aagtgccgaa acgaaacgac ataaagaaaa tcctgctgat
     2221 cggttccggt cccatcatca tcagtcaggc ctgcgaattc gattactccg gaacacaggc
     2281 ctgcaaggcg ctgcgggaag agggttacga agtggtctta atcaacagca atcccgccac
     2341 catcatgacc gatccggaaa tggccgatcg cacttacgtc gaacccatca caccggaagc
     2401 cgtggaaaaa atcattgccc gcgaactgcc tgatgcgctg ctgccgacgc tgggcggaca
     2461 gacaggactc aacacagctg tgacgctggc tgaaagcggt gtgctcaaga aatacaatgt
     2521 cgagatgatc ggcgcctcgc tggacgtcat tcacaaggcg gaagaccggg agaaattccg
     2581 gcacgccatt gaaaaaatcg gtttgcgcat gccgaaaagc ggttttgccc gttcaatgca
     2641 agatgttctg aaaatcgctg acgacatcgg gtttccgatt atcgtccggg cgtcgtttac
     2701 cttgggcggc accggcagcg gtgtggccta taaccgggaa gaactgatgg aaattgccaa
     2761 ggcgggactt gatgccagca tgatctccga ggtcatgatc gaagagtcgg tgctgggctg
     2821 gaaggaatat gaactggaag tgatgcgcga caaggctgac aacgtcgtga ttatctgctc
     2881 cattgaaaat ttcgacgcga tgggcgtgca taccggtgaa tctatcacgg tcgcaccggc
     2941 gcagacgttg acggacaagg aatatcagaa catgcgggac gccgccattg ccatcatgcg
     3001 tgaaatcggc gtagagacgg gcgggtccaa tgttcagttt gccgtgcatc cgcaaaccgg
     3061 agaactgatt gtcgtggaaa tgaatccccg cgtttcacgt tcctcggcgc tggcttccaa
     3121 agcgacagga tttcccattg ccaagattgc ggcgaaactg gctgtcggtt acacgctgga
     3181 tgaaattccc aacgatatta cccgcgagac gatggcgtct ttcgaaccga cgattgatta
     3241 ctgtgtcgta aaaattcccc gctggacatt tgaaaaattc ccgcaggccg aggattttct
     3301 gactacatcc atgaaatccg tgggtgagac catggccatt ggccgcacat tcaaggaatc
     3361 actgcaaaag gccgttcggt cgctggaaat cgggcgtttt tctctcgaac aggctctgcc
     3421 tgaccatata ccggataaaa aagaatttct gaaaagcaaa ctgatcaagc ccaattcgct
     3481 gcggcttttc tacattgccg cggctatgca aaacggcctg tcgatcgaag aactttatcg
     3541 attaaccaaa atcgatccct ggtttttgca tcagatcagg gaaatcgttg atgccgaaaa
     3601 tgaaatcaaa gaatcaaccc catcggtgga ggtgctgcgc gaggctaaaa aaatgggctt
     3661 ttccgacagg gcgttggcgt ctttttggaa aatgacggag cctcaaatca gacaatggcg
     3721 cgaaaaggga aaaatcattc cagtgtataa actggtcgat acgtgcgcag cggaatttga
     3781 agcctacacg ccttattatt attccacgta cgagacggag aacgaaaccc ggctgtcgga
     3841 taaacgcaaa gtagccatta tcggcggcgg accgaatcga atcggtcagg gcattgaatt
     3901 tgattattgt tgcgttcacg cctcgtttgc cttgcgcgag gaaggcgttg agagcattat
     3961 gattaattcc aatccggaaa cggtcagcac cgattacgat acgtcggata aattgttttt
     4021 cgaaccggtt acgctggaag acgttttgca cattttacag acggaaaaac cggaaggtgt
     4081 gatcgtgcag ttcggcggcc agacgccgct taatctggcc aaaggcttgg aaactgccgg
     4141 cataaatatc ctgggaacat cgcccgatgc cattgaccgg gccgaaaacc gcgaccgctt
     4201 ccaggaaatt gtgaccaaac tcaaactgaa tcagccggat aacgacacgg cacgagacac
     4261 acaaaaagca ttgcaggccg ccgagcgtat tggctatccg gtgctggtgc ggccttccta
     4321 cgtgctgggc gggcgttcca tggaaatcgt gtacgatgaa gaaacgctga agtccttcat
     4381 ggaaaaagcg ctgctggtct cgccggaaca ccccatcctg atcgacaagt ttctggagga
     4441 cgctgtggaa attgacgtgg atgccgttag cgacggcgag accacggtgg tggccgggat
     4501 catggagcat atcgaggagg cgggcattca ttccggtgac agcgcctgca tgttgcctcc
     4561 cttgaccatt cccggtgatg ttttggatct gatccggaaa caaaccacca tgctggcgaa
     4621 ggaattgaat gtgatcggtc tgatgaatat tcaatatgcc gtcaaagacg ggaaacttta
     4681 tgtgttggaa gttaatcccc gcgcttcgcg aacggtgccg tttgtcagca aggccatcgg
     4741 tgtgccgctg gccaaactgg caacaaaagt catgctgggc aagtctttaa aagagctggg
     4801 atttaccgag tccccccggc caaaacatat ttccgttaaa gaagctgtct ttccgttcaa
     4861 ccggttcccg gatgtggata ttttgctcgg gccggaaatg aaatccaccg gcgaagtcat
     4921 gggcatcgat caatctttcg gacttgcctt tgccaaatcg caaatggcga ccggattcaa
     4981 aataccgcag caaggcactg tcttcatcag cgtcaatgat caacacaagg ataggattgt
     5041 aacggtcgcc aaaacgtttc agaagatcgg ttttaaaata ctggcaacga aaggcactgc
     5101 cctgtacctt ttggaacacg gtatcagcgc cggtgttgtt ttaaagttaa gcgagggccg
     5161 accgaatatc gtggacttca tcaaaaacgg ggacatgcag attgttatta acaccagtat
     5221 aggacgaaaa tcttcgaggg atgcttatca tatcaggcac agcgcgctga tatataatat
     5281 cctttataca acgaccattg ccggagcgcg cgccctggct gaagcgacag tcgcaataaa
     5341 agaaaaggcc tggcaggttt gctccctgca ggaatatcaa aaagcataaa acagcttatt
     5401 ttctcacctt tgaaggttat ggtgtcctat agggtagaaa agagtgacaa ttaaaaaata
     5461 gcctctcccc tcgatttgtt accgcgggga gaggaaaaaa tcagaatgcg agagtaatga
     5521 aaatccaaaa ggttttttga taaaatgtta tcggaaaaag aacaggaaaa gcgatattat
     5581 ccccgcgaag aatgcggtat ttttgccact tacggtcatg aagaagcatc ccgcgtagcg
     5641 ttttacggtc tttttgccct tcagcatcgc ggccaagaat ccgccggcat cgtcagctct
     5701 gacggctgca atgtatacga gcacaaaggc atggggctgg cggccgaggt tttcaaggaa
     5761 gaaacgctcc aaaagctgcc cggcaagctg gccatcggac atgtccgtta ctccacgacc
     5821 ggttcatcga atctttgcaa tgcccagccg tttttggtgc attacggaaa cgaatattac
     5881 gctctggccc ataacggcaa tattctcaac gcacaaaaat tacgcaggga gcttgaagac
     5941 agcggttcca tctttcaatc caccatggac agcgaagtga ttatccatct gatggcacat
     6001 tacctgaaac agggcctcga gaaagcgctg gcggctgcac tatcgcgact ggaaggcgcc
     6061 tattgcctga ttatgctgac ccgcgacaaa atcatagccg cccgtgatcc gcgcggattt
     6121 aggccgctgg cgctgggcaa agtgagcggc aaatgggtga tcgcctcgga aacctgtgcg
     6181 ttcgacctgg tcggcgcaga ctatgttcgc gatgtagagc ccggagaaat tttaatcatt
     6241 gatgccacgg gcgtcaaaag cgtcaaccct ttttccgcga tcaggcactg ccattgcatt
     6301 tttgaactga tttatttcgc ccgtcccgac agtcaggttt ttcggcagaa tgtttatcag
     6361 tatcgcaagc ggcttggaca cgaactggcg cgtgaataca aaccggatgt tgatatcgtc
     6421 atgccgtttc ccgattccgg cgtttacgcg gctttaggtt atgccgaaga aagcggccgc
     6481 ccttttgaaa tgggtatgat tcgcaaccac tatattggcc gcacctttat tcagccttca
     6541 caggccatgc gcgatttcgg cgtccgcgtg aaactcaatc ccgtcagagc cttgctggaa
     6601 aataaaaaaa tcctgatcgt agaggactcc atcatccgcg gcacgaccag ccgcaaccgg
     6661 gtcaagaatc tgcgcaatat cggcatcaaa gcaattcaca tggcggtgag ctgtccgccg
     6721 acggtctatc cctgtccgta cggcattgat ttttcatcca aaggagaact tgtcgcctcc
     6781 aagatggaaa gtgaaaaagc cattgctgat tttatcggtc tggactccat tcattatctg
     6841 tccgtcgaag ggatgatcag ggcgaccggc ctgaaaaaag actgcttttg cctggcctgc
     6901 tttaccggtc actatcccat tgctccgccc gaaatccttg ataaattttg tctggaaaca
     6961 aaatagtttt tccggataaa ttcctgcctc gattgtcgtg agcaattgaa attattgatt
     7021 gataattggt tgaattgaat ttgaccccaa ttccttccaa taaggataat tactgattga
     7081 aaaaatttcc ggagcacatt agattatata acggtgacgg agcatatcac ccgttgaaag
     7141 atccggaaaa gttaaccggg caatcaacga tttattcata cgtccgtttt gcagtaatct
     7201 gtctgaatgg atagttcgta aacaggagaa atgatgggaa tacatacata taaagcggct
     7261 tcagttttaa aagaaacggg aatgatggtg gaaagcgacg cgaggggatt caaatttatc
     7321 gccgatgaac ccagggggct cggcggaacc aataggggaa tgaaccccat ggaaaccatc
     7381 ttgtgcgccc tgggcgcctg ccagtgcatg acggcccgtt tttttgccat gctccttaaa
     7441 atcgatctta ccgaatacca cgtggatgtt gagggagata tcgatgccag gggctttctc
     7501 aatgccgatt cgggcatccg tcccggcttt caggaaatcc gcttgaccgt ccgtatgaag
     7561 gccaatgccc ctgaggaaaa gatcgctgaa ctgctggcta tggtggaaag ccgctgccct
     7621 gtcggtgaaa ctgttctcca tggtgcgcct gttgtcgttc ggcatatcga attattatag
     7681 ttgacgatca acgaactgac ctcgggaact tataaatgaa gaatcttgtc tttagcattt
     7741 tcctcaaaga acgtatcctt gtggacgaca ccctttagta acattttatt tgagataatt
     7801 cggtggttga gatttcctcg catataaaga tattttggac tgtcccccac ggattagaca
     7861 aaataatgga ttgaattgat attgatggat acaaggtatt gattggttca aatttcttgt
     7921 tcttcaggcg ttctgcatac taggccagaa tcatgtcaag aagtttcaga gctgcttctt
     7981 tcgggccggt catttcacat ccgggtatac ccaggcgtgt acggatttgc ccgacgaaga
     8041 tgcccttcct gaagaatgct tcgaaagatt tacgcgcgag ctgatcatat attttccgat
     8101 actgaggcgg gccgaaaatg ttggcgaaat acgagtggac gatccctttc gatgtggtgg
     8161 tccccttgct catgaccgtg ccctccctga acactgaaaa cgcctgatcg ggtgttgaga
     8221 agcgttcgag tgccggatgg tcgtcgtcaa tttcctcata gttattggcg tagagaacgt
     8281 attcgatccc gacccccatg ttgacctctt cgagggttga aaccggcaat acgatacgtg
     8341 cgtttgtttt cccggggctc atgatgatcg accggtcgat gttcctgaaa gcgtatccgg
     8401 gcttgatgtc gtcaagcctt ataaacgcgc ctgtttcggt cccatatccg attatatcgc
     8461 cttgctccgt aatgttgatg gaacccatat cgtctgcgat aatctgcagt ccacgcaggt
     8521 gctcgtcccc gagcatcctg aacgcctcca gtgtttcgga tttgcccgcg cccgaatcgc
     8581 ccatcatgag aatggtcgct tcggtcccgt tcttcaaatg aatctgatag agcgcgccgt
     8641 ggaaagggag tctcccctgc ctgatcatga tcgcgttatg caatgtaagc atcatcttct
     8701 tgagatagcc gaaataaccg aactcgctgc ttcccggaca agctccgatc aacaggttgt
     8761 tggaccggtc ctcgtgaaac atggtcggat attgtgccag tccgtcaagg gacccatcct
     8821 gaacgccgaa caggaaaatt gcatccggtt tcttcgcgag atgcttgtcc ccggcgagtt
     8881 cgaacaggtt gcagagcgaa aaccccagat ccataaaatt ttggtggaaa tagacatgca
     8941 tgagaagatt tccgaccatg gcggggtagc agagccactc atccggccgg atatcaacta
     9001 catccatagg gttcgtgtcg atgcggacga actggccggt ccgcttgttc atgggtggat
     9061 cgaagatcaa agtcggatag aataatacct gtcgaatcat cggtaccgat gagagcttgt
     9121 ccgcgtaaac gccggagaga tgaatatcct ctctggtgca gatggcggaa atcttggccc
     9181 cggcagacag ctgccggtat atgcggggat gtgatccggt aatattttcc tggatatccc
     9241 gatatgtgtc tcggattaat tcagtgaggc cctgaatcct ctggttgaac acccggtagg
     9301 gtcgtttctc gaaactatcg tcaaatgaat cgattataat gtagcgttca tgagagcgcc
     9361 agaaattgta tagatattcc acgaatttgt atagattgtt gatgtcggaa aaatatggtt
     9421 cggttccctc aactactttt ggaataagtt cagcctctat cttggcaaga tttttaagca
     9481 ccagtgccat tttttgtata gtctgctgat caacggtcgt ttttccgaat atgttcagaa
     9541 gcagcgaatt tcgcgattcg agttccctga tacattctgt taggattctg aagaacagat
     9601 cgctttccag gagttcgtcg ggggtttcgc aaagccggcc ccctgttcgc atgattattt
     9661 tattgccgat gactgaataa tctatagcca tggttcccct cctttcgttc gtcgtttcgt
     9721 tgaaaaatat cactgttccc ccgatgaaat caatcctttt ctcaaggggc atatgccgca
     9781 agtgatatct tgataacaga ttgaacgatg cgtataccgg agtggacgtt attttttcag
     9841 tcgatattgc tatcgaaatt atcatagtcg tccatctgtt gttccttgcg tttttattgc
     9901 taaaatttcc tttaaataaa ggatatgaca aagagaatgc cttcccagtg gtgttcggct
     9961 gatagacatc gacgtcaaat ttgtgtataa taaaacacat tttgagggcg tttgtgcatg
    10021 tcaaaaaaac ccaagaaccg gtgtcgctga taaataaaac ttcaaggaga agcgtatgaa
    10081 agaagggatc gtatgcaaat ttggcgggac atctgttgcc aatgccgcac agatcaacaa
    10141 ggttaaaaat attgtcacaa tgaacacgaa acggcaatat gtgattgttt ccgcaccggg
    10201 tagggattct tcggatgatc agaaagtaac tgaccacctg ttgaatgttg caacgaatgg
    10261 cagacatttt cgcctgcaga acaaaaacat cacgggtcag caaagttacg aagctgttct
    10321 ggggaaattt cagcggctga ttgacgagtt ggccattgat ggcggcgata tattaaaaaa
    10381 cctcgaaaaa gatatgaaaa cacccctccc ggatgttaag cggcttgact tcatggcatc
    10441 cagaggtgaa cgctataacg ccgaggttgt gagccgttac ttgaagaaac aaggcatacc
    10501 ttcagaactt gttcttcctg aggaaatggg tttgacggtc acgggcgatt tttcaaacgc
    10561 ccgggtgatt ccagtcagtt atcggaatat caagaaaaaa ttgagcagca ttgaaggggt
    10621 atccgtcatt cccggttttt acggggtgac cgaaaaaggc gacattgctg tattttcacg
    10681 cggcggttcc gatctgacag gcggcgaggt cgccttctcc atcgaggcgg gtctgtacga
    10741 aaactggacc gatacggacg gcatctatcg ggcggatccg cgcctgattc cggaagcgaa
    10801 ggtgatcccc cgccttacct ataaagaaat ccgtctgctt tcatcaaagg gcttcgatgt
    10861 gtttcattat gatgccatgg tgaattgcaa gaaaaggaat attcctattc agatccgaaa
    10921 taccaataac acgaattctg aaggcacgct tattgtatcc gaacgggttc ctgaagaagt
    10981 tgtcgtcggt atagcccgta aagataatat tgcttttatt tatatagaac gtgacggagc
    11041 aggagaaacg attggttttg tgcacgatct tctgagtgtt atcaaggatt atgggattga
    11101 gacatatcat tacccgacag accgggacga tatgaccatc ttgatcaatc aggatgacat
    11161 ggtcggttac gcagaggaca tcaaggaacg gatagaacgg gagttcaaac ccgataccct
    11221 tgaattcaat tataacatct ctattctttc tcccgtaggc ataggcatga aaaatcatcc
    11281 cggcgttatc gcggaagcgg cgaatgcatt gcgtgataag aacatcaaca tcgatatcat
    11341 catccaggga cctgcgcaga taagctttca cttcggattt cagaacttct atgctgattc
    11401 cgccctgcgc gccatgtata attgtctgat cgaataaagc gtcagtgatg atagctgatg
    11461 aagtgcaagc ttttggggcc ggcgatgacc ggcgttgccg tttcagtcgc aaaagtttca
    11521 aagtaatcct ccacgcaatg ataataatcc gaatcctgcg gtcggcgctg gtatgcggct
    11581 acgggcaatg gcatataatc ctcttccata aaacggataa atgcattccg aagccacaag
    11641 acttaatagg acaaaagtcc tattgttaag aagaaaataa tgatgtgtca aatcgtacag
    11701 tgttttcgat atagccattg cgctcatcgt gtctatatgt taagcaaatc agcaacgttc
    11761 accatccgga gagattttga aaaatgaagt acgaaccgtt agttatcgaa gagaaatggc
    11821 aaaagaaatg ggaggatgaa aaatccttca aggttaccga agatccgcaa aagaaaaaat
    11881 attatctgct ggagatgttc ccttatccct ccggcaaaat tcatatcggt catgtccgca
    11941 attacaccat cggcgatgtt gttgcccgct acaaacgcat gaaaggtttc aatgttctgc
    12001 atccgatcgg ctgggatgcc ttcggcatgc ctgcggaaaa cgccgccatc gagcataaga
    12061 ttcatcccgc gaaatggacc aacgaaaata tcgagcacat gaaaaaacag ctcaaacgca
    12121 tgggcttcag ctacgactgg gacagggaag tttccacttg cgagccgaaa tattaccgat
    12181 gggaacaact tttctttatc tggatgtacg aaaagggact ggcttacaag aaacgctcca
    12241 cggttaattt ctgcaccaag tgcgatactg tgctggctaa cgaacaggtc gaaggcgggc
    12301 agtgctggcg ctgcggcacg gaagtgcagg agaaggtgct ggatcagtgg tttttcaaaa
    12361 tcaccgcata cattgaggaa cttctggatt actgtgacaa gctgcccggc tggccggaac
    12421 gcgtcatgac gatgcagaaa aactggattg gcaagagcta tggctgcgaa gtggacttcc
    12481 cgatggccga cggcaacggc gccatcaaag tattcacaac ccgccaggac acgctcttcg
    12541 gcgcgacctt catgctggta gccgcggaac atccgctggt tatggaactg accaaaggca
    12601 aggcagtaga aaaagatgtc atgcagtttg tcgaaaaagt caaaaagcag gacaaactga
    12661 tgcgcacttc tgaatattac gaaaaagaag gtctcttcct tgattcgtac tgtctcaatc
    12721 ctctcaccgg tgggaaaatg ccgatcttcg cggctaattt tgttctggcc gattacggca
    12781 ccggctgtgt catggccgtg cccacgcacg atcagcgcga cttcgagttt gccaaaaaat
    12841 tcaacctgcc tttgattgtg gtcatttcgc cgaaagacag aacgctcgat gcggcgacga
    12901 tgactgaagc ctacgttgat gaaggcgttt tggtgaactc cggccagttc aacggtatgg
    12961 aaaacaccaa ggttctggaa gcgattgccg attttctgga aaaggaaggg aagggcaaac
    13021 gcaccattca gtaccgtctg cgcgactggg gcatttcccg ccagcgttac tggggcgcgc
    13081 cgattccgat gattaactgt gctaaatgcg gcatcgttcc tgttgatgaa aaagacctgc
    13141 cggttatact gcctgagaat gttaagttaa gcaccgaagg cggttcgccg ctcgcggcgc
    13201 tgaaagaatt tgtggagaca acctgtccga aatgcaaagg tccggcctcg cgtgagacgg
    13261 acacaatgga tacattcgtg gagtcgtcct ggtatttcga ccgttactgc tgcccggatt
    13321 acgacactaa acccggtctt gaccgcaaaa aactggatta ctggatgccc gtggatcagt
    13381 acatcggagg catcgagcac gcgattctgc atctgcttta ctcgcgcttt tataccaagg
    13441 tattgcgcga tttcggcgtg atcggtgtgg acgaaccgtt caccaatctg ctgacgcagg
    13501 gcatggtctg caaggaaaca atgaaatgca aggagcacgg ttatcttttc cccgatcaag
    13561 cggcagatgg taaatgccac atctgcggga aagacgttat tatcggcaag acggagaaaa
    13621 tgtccaaatc gttgaaaaac gttgttgacc ccgattatct ggtcaagaag tacggtgccg
    13681 atacggcgcg gatattctgc ctgtttgccg cgccgccgga gaaggatctg gaatggagcg
    13741 atcagggggt ggacggctct ttccgttttc tgggacgact gtggcgtatt gttgatgaat
    13801 atctggaaga catcaaaggc gtggctcccg caaacggcgc gttgcaactg gaaggcgatt
    13861 taaagtctct gcgtcgaaaa acccatcaga ccatccgcaa ggtgtccatt gatattgaag
    13921 accgcttcca ttttaacacg gctatcagcg ccgtgatgga actggtgaat gttgtttatc
    13981 aggtcaaacg tcccgcaagt tccgataagg ttgctttatc tgtggttcgc gaggcgttgg
    14041 aggcggccat tattttgctg gttccgattg tccctcatgt tacggaagaa ctctggcagt
    14101 tgctcggaca tcaaaatctg gcatcagaca tgtcctggcc cgagtttgat ccggctattg
    14161 ccagcgaaga ggaaatgacg attgtcgttc agattaatgg aaagctcaga agccggatga
    14221 ccgtgccggt ggatgagagc gaagaaaaaa tcaaagcgga tgcactggca gatgaaaaaa
    14281 tcaagtctat gaccgccggc atgcaaatca agaaagtaat ttacgtgccg aaaaagctgg
    14341 ttaatattgt agtgacgcta tctgagagct agcggagcgc cgctcgtccc tgccacctga
    14401 aggtggcggg ataattcgtt ttaagactat agtcaaggtt cattaaagag taagttaaac
    14461 tattcccgaa gcgaagcagg attaagattt atcgcggtca aatgaaatta gcaaagatat
    14521 ttcgacacag ttcaaaagct ttgtaattaa aggtattagg atgaaaaacg aaattggttt
    14581 aataactaaa atcgcaataa tttgtatttc ctgcgccgtt ttattcggct gcggttattc
    14641 ctttgctccg cggggagaat ccatcgatac ccgcattcaa aaagtttacg tggagccctt
    14701 tgaaaataac actgcgcagg ctgaaataga aaattatgtc cggtcggctt ttattaatca
    14761 gttcatacaa aacagccgtt ttaaactggc ttccggcacc gagacggctg atgcggtcgt
    14821 cagaggaaaa attgtcaatc ttcacacctc atcgcttacg catttgaaaa acgatttggc
    14881 ggcagaagag cgggcgacta tgatcatgga agtggtcttt gaagacaaat ccaacggcag
    14941 aattatctgg agtgcgaaaa atataacgga ctcggttgac tacgttttaa atgaagatat
    15001 taacatgctg cctgcgtcaa aaaaacaggc ttttatgaaa ctggccgacg acatggcgga
    15061 aaaagcattc aatctgatga tgtccggatt ttagtgagcc aagccgaaga cgaacgaagt
    15121 gaagtatgag gcgtaaggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 aactatcctt gaagcgcagc ggagcgggat ttaagccaag ccgaagacga acgaagtgaa
    15301 gtatgaggcg taaggcgaac tatccttgaa gcgcagcgga gcgggattta agccaagccg
    15361 aagacgaacg aaatgaagta tggggcgtaa ggcgaactat ccccgaagcg cagcggcgag
    15421 ggataatgag cacattttta ttatcccgat agtggagcgt atcgggataa ggtacgattt
    15481 aatatgacac cttcagccag acaattcggc acgttctttc ttctcccctt tactgacgtg
    15541 caagaggctc ttgattttat ttcctccaat aagcgttccg tcacagtcat aaaaaacctt
    15601 tccgcctatc ttgcaaggtt atcctgcaaa tatctgctgc tggtcgatgc cgaaaaagac
    15661 atcagcattc gggaatcgtc tttgcgacat tttctaaaac catttacgga tcagcacacc
    15721 ggcatggttt attctgattt cacctcgaaa aaacgaaatc ttctgaaaaa acatcccgta
    15781 attgattacc aggccggcag catccgtgag gattttaact tcggctctct tttgattttt
    15841 tccgttgacg ctgtccgaac ggtattgcaa aaatacggtg cttcaccgtc cgatccggat
    15901 gtggcgttgt acgacctgcg tcttaaaacg tctgcagact atcacatccg tcatgtgcgt
    15961 aaattcttgt acacggtttc cgttaaaaac cataaacagc tcgcagcctc cggcgggaaa
    16021 gcggaaaccc actttgctta tgtggcaaaa gaaaatttcc tgcgccagaa gaaactggaa
    16081 aaaattgcca cccatcacct gaaacgcatc ggggcatatc tgccttcgcg tacaaaaatt
    16141 gtgaataacg aacaggatgg cggccagtgg aaagccagta tcgttattcc cgtccgtaac
    16201 cggaaaaaga tgattgccga tgcgctgaaa agcgccctga aacagaaaac cgatttcccg
    16261 ttcaatgtca ttgtagtgga taaccactcc accgacggaa ccacggatat tctgcggaaa
    16321 tttgcatcaa cttatcccca cattcatcat ctgattcctt cgcggcgcga tctcggaatc
    16381 ggcggttgct ggaatggagc catttattcg ccgtattgcg gccgttatgt ggttcaactg
    16441 gactcggatg acctttacag ctcgccgcat acgcttcaga aagtcgttaa taaattgaga
    16501 cgcggacgat acgcgatggt ggtcggttct tacaccattg ttgacgaaca tttgaaaaag
    16561 attccgccgg gactgatcgc gcataaagaa tggacgcaga cgaacgggca taataatttg
    16621 ctgcgcgtca acggcatggg agcgccccgt gcgtttgacc ggtctgttat ttgccggacg
    16681 ggtttcccca atgtcagcta cggggaggat tatgcggtgg cattgagaat cacccgtgaa
    16741 tataaaatcg gcaggatcta tgaaaatttg tactggtgcc gacgatggaa ggataacacg
    16801 gatgccggtc ttgacgcgga gaaaaaaaac cgcaacgatt tttataagga tcaactgcga
    16861 acactggaaa tagaggccag aaagtcggcc aacaaaggag acgcgtgtgt aaacagccgg
    16921 agagttttag ctgaattttc ggggggcaag gctttagcat tgccttttct ttgtcaacat
    16981 ctttatgatg cgcagaagaa aacctggccg aaactcgctg aagcttgtcg gaatttggcg
    17041 tccgttcata aaagaaagct tcccggcgcc tatgacgttt atctgcaata taatcccgcc
    17101 cgcgcgatca gcagcggcgc atctgttgat gcccagtcca tcagacaaag gccctgcttc
    17161 ttatgtcagg aaaatcttcc ggtggaacag aaagggattt tgtacagaag ccagtatctg
    17221 attctctgca atcctgcgcc gatctttgag aagcatttta caatcgtatc cttgcaacat
    17281 acgttgcagg aaattacgtc gtcaataaac gggtttttgc aattgagcgc cgatatgtcc
    17341 ggttacgctg ttttttacaa cggtccggcc tgcggcgctt ctgctcccga ccatttgcat
    17401 tttcaggcgg ttcccaaaaa atttcttccc ttcctccggg agattaaaaa actgcctgtt
    17461 tgcgatacat cgtctttgcg atatgtgtcg ggcgaaacgt tcgatcgatc cgttgttttg
    17521 ctggaatccg gggatcaaga cgctttgagc atgcaattcg agacgttatt aaaggcgctg
    17581 caaagcatca tgcagacgaa agtcgagccg atggtcaacg ttgtttgtga tcatgcggga
    17641 catcgctggc gtctgattgt ttttctgcgt caaaaacatc ggccggatgc ttattttgag
    17701 aagggggaaa agcgaatttt tatttcgccg ggagccgtgg acatggccgg cgtcgtgata
    17761 acgccactgc tggataatta taaccgtctg gattataatg ctgtccgtga aatttaccgt
    17821 gacgtatcgc tctctgaaaa cacaacggac gcggtcatta gaaaattata aaaagaaggt
    17881 tgcgaatgtt ttttgccgaa gaagcgattg tggacagcgc gatgagtttt tccgaagcga
    17941 ttgaagggac aaccgctccc gacgaaatca ttctttcatt gtcgcttgtg gatgtgtttt
    18001 attattcttt tgacgggaga aagcatcagg gacagatgct catcagcaaa gaattggaag
    18061 acgatgctta tgacatcttc aacgtcatcg aaaaaatttt cttcccggta gacaaggtga
    18121 ttcccataac ggcctatcaa tggaatgatt accggtccat ggcggacaac aatacgtcct
    18181 ctttcaattt ccgggtgatt gaaggaacca ctaaattatc catgcattct ctgggtaagg
    18241 ctctggatat caacccggtg cagaatccgg tgatttatcc caacggcgtg gtggcgccgg
    18301 aaggagcgaa ataccagccg gatgaaaggg gaaccttttc agcggatcac ccgattgttc
    18361 gggaattttt aaagcgcggc tggcactggg gtgggaattt taatcagccg aaagactatc
    18421 atcacttcga aaaaccatga tgccgacgtc aaatcaaaat actatcagct ccgttctgat
    18481 gtcttctttg acttgaaatc gatctagaat actgatcggg cgacaataat gccggtgaat
    18541 tttttgttgg atgtcagata agccaacagc gtttttttgg aaatcaccac tttaccttcc
    18601 tgacgggaag catgaagcag acgtaaactg tttccttgcc acagagcaaa accgacatgc
    18661 gccacatcaa gcccttgctg atttgaggca aaggcgatca catcgccgct ttggattttt
    18721 tttatccgcg ttccgagctt ctctttattc attacaggaa aagccttccg ggaaagcatc
    18781 ttttcgatat cgattatttt ggccagttgt actttgtttt tcagagccgg atataattcg
    18841 ggatgcgtgc tcatgaaatc gattttttta cggtgcgggg ttgcttgcag atggccggtt
    18901 atatcgatga gaaatttctt ttcaatattg ttgcgcatcc agtcggtaaa ataatgcagg
    18961 cgggacgaat atccgctgat ttttcccccg cggtaacgaa tcaatttaag atatttttta
    19021 aaagctccgg atgaaaatct gccggaagcc gcgcaccgga aaaaagctaa aacggtttca
    19081 acaaatgtcg tacaatcgaa agctgatacg ttaatgatta atttttcctt gccgggattt
    19141 tcaagcattc catcccggta gggcttgccg ataaaaagcc gtcctatgtc cactattaaa
    19201 tcgcctaacg ccgcttgatc ggatgtactt tcgttcatgg acattccttt tatttatttc
    19261 taaatgatta tcgaggacaa agcaaatata aatatttgag aagaggagaa aatgaatgcc
    19321 tgaaaaggaa aggccggttt tataaccggc ctttccttaa tttgtattgt tgatcatctt
    19381 ctgctacgga atttctttca acttcgaccc attcggattg aatagttcaa cggaccaatt
    19441 ttaatgcgcc tatagtgacc ccatggtgtt cttcaggtgt ccatgttata cgcttttaaa
    19501 attggagcca aactacatgg cgttgacttt tttggtcaga cgggaaattt tgcgggaggc
    19561 gtttttctta tgaatcaagc cctttgctcc cgctttggca atagcgggaa tggcagcctt
    19621 caaggctttt gcggaatttt ccttattttt gctttcaacg cccgcaacga ccgatttgat
    19681 ctttgttttt aaagccgact tggcggcaat attgcgttga cgatgttttt tattttgccg
    19741 gtcgcgtttt tctgctgatt tatgtgttgc caaaatatcc tcctgaattc atttataggt
    19801 gcggttatct agcccaatta ttgaagactg tcaagtataa actgagtcat tatagcttgg
    19861 tatcctttct ccctatcaca aaaaacacta tcattttcga tgggatggca tatttaaggt
    19921 ttaaaaactt ataaaaattt ttcttgacat aatatccttt cttcaatatg caactcggtt
    19981 atacactcac aggaaactat ggtttcatct attttctgcg acgcaatatt caaagattag
    20041 ttgaatggac aatgcatttt taatgtattt gccgttaatt taatgtggtt atgtgactat
    20101 agccgtgaaa cagcagtcgg gaaaaaaatc cattaataag agaaaagttc tccagaacga
    20161 cctgtctatt ctggccagag atatcattgc aaacgccggc gtcggaatgt atatcgtcca
    20221 ggacggcaaa tttgtttatg tcagcgaatt atatcagaaa attaccggct ttacggaaga
    20281 agaactaatc ggtacatatc cgctggataa tattcatccg gacgatatga aaaccgtcag
    20341 agaaaaggcg ataaaatgtt tgaaggaagg gaactgcaac ccttacgaat acaggttcat
    20401 tacaaaaagc ggtgaaataa tctgggtgct ggaagcggtc acctccatta gctttaaagg
    20461 ggagcgggct gccctgggca gttttataaa catcaccgaa cgcaagctca tcgagaaaaa
    20521 attgcaccat gaggagcaac tgttcaggac acttacggat caatcttcgg atatcatcgc
    20581 gattattgac cggaaaggtg ttattactta tgaaaataag gccattgaac cggttctggg
    20641 tttgaaagtc gatgaacaga tgggcggcaa gggctttgac aatcttcatc cggatgatgt
    20701 gccgttcgtc tccaatgaat taaaaagact gtttgcggat ccggatgctt ctgcttcgcg
    20761 ttcggaagtt cgtctccgcc acaaaaacgg tcagtggctc gtgtttgaat ttatggccag
    20821 cggtttggtg aacaataatg ttgtggaagc ggtgatgatc aacctgcggg atattaccga
    20881 acggaaaagg gctgaggagt cgcttcggtt cagcgaggaa aaatacagaa ccattctgga
    20941 aaacattcag gaaggatatt ttgaggtcga tctggccggt aatctgactt tttataacga
    21001 tccggtttgc gaaatcatgg gctataccag agaagaattg atgtccatga attataaaaa
    21061 gtacaccggt aacgaaatgg cggtgaaggt ttatcaggca ttcaataaag tttacctgag
    21121 tggcaattcc accaaggaaa ttgactggca gattatcaga aaagacggag ccaggcgcta
    21181 tattgaagct tcagtatcgc tgcaaaagga tttttccggc catccgaaag gattcaaggg
    21241 catcatccgt gatattaccg agcgcaagga aatggatcag aaattgaatc atctggccac
    21301 acacgatgca ctgaccggtc tgcccaatcg aatcctgttt atggatcgtc ttcaggttgc
    21361 gctggtgcaa tccagaagaa accggaacaa actggccgtc atgatgcttg acctggatca
    21421 tttcaaggat gtgaatgaca cgctgggaca tctggcgggc gacaaactgc ttcaggaggt
    21481 gggcaatcgc ctgacggata ttttacggca taatgacacc gttgcgcggc tgggcggtga
    21541 cgaatttgtg gttttgttgt cggatcttga aagaatggaa tacgcagccg gcgtggccaa
    21601 agtcattttg aaagcttttg ggaaaccttt tttactggcc gatcatgaaa ttacacccaa
    21661 cgtcagtatc ggcattgcct tcttccctga tgattgtgat gatgtcgaat cgcttttgca
    21721 aaaatcggat atggccatgt attcggttaa gacgcacgga cgtaacagct ataaattcta
    21781 cagccacatc gcgtcttgaa aaccaaacat tgttacgttt tggagcggga atagttcagc
    21841 gcggcttcga taaatttgct gaaaagcggg tgaggtttga tcggtctgga tttgaattcg
    21901 ggatggaact ggcatcccag aaaccaggga tgatctttga tttccacaat ttccgccaaa
    21961 cgtccgtcgg gtgaaatccc tgttatgcgc aatccctgat tcatgagcgc ttctttgtaa
    22021 tcgttattga attcgtaacg atgacggtgc cgctccgaaa tttcaccctg accgtaagct
    22081 ttgcaggcga aggagtccgc ctcgacaagg cagggccacc cgcccaatct catggaggcg
    22141 cccttttctt tgacatggcg ttggtcgggg agcagatcaa tgaccggata ggacgtctcc
    22201 ggatcgaatt cggaactgtt tgctttctcc agtttgcaaa cgtttctggc atattcgacg
    22261 acagccattt gcatgcccag acaaatgccg aggaagggaa tctttttttc tctggcgcgc
    22321 tgaatggcga gaatcatgcc ctcaataccg cggataccga agccgccggg aaccagaacg
    22381 gcgtcgatat cgtccagatg tttgcccagg ccttccgctt caattttttc cgaatcgata
    22441 aatttcagtt tcacgcggca gttgttggca atgcccccat gcacgagcgc ctcgttgagg
    22501 cttttgtagg aatccgtcag attcacgtat ttgccgacga tgccgatcgt cacgtcatgc
    22561 aaagggttgt ggaacttatc gaccacatcc tcccaaacgt cgagtttcgg ctgtcctgtc
    22621 cagatgttga gaagatcgac aatcttcgca tccagtccct cccggtgaaa aatcagcggc
    22681 acttcgtaga tgcagctgac atccttggcg gtaaagacag aatggacctc cacgttacag
    22741 aaaagagcaa tcttggattt gatatcatcg gaaaggaaat tttccgtccg gcaaagcagg
    22801 atatccggct ggatgcctat ttctctgagc gccttgacgc tgtgctgagt gggcttcgtt
    22861 tttacttcac cggctgtctt gataaggggc acccaggtta aatgaatgaa aatggcattt
    22921 tctctgccga cttcattgcg gaactgccgg atggcttcca ggaaggggag gctttcaata
    22981 tccccgacgg tgccgccgat ttccacaatg gccacatcaa aaccgtctgc tgtgtcgcgg
    23041 atgaaatctt ttatttcgtt ggtaatatgg ggaatgacct gaacggtttt ccccagataa
    23101 tctccgcgcc gttctttagt gatgacggcg tagtaaacct gtcctgtggt caggttgttg
    23161 cttttgccca tgcaggtgga acagaatcgc tcgtaatgtc ccagatcgag atcggtttcc
    23221 gcgccgtcat cggttacgaa cacttcgccg tgctgaaaag gactcatggt tccgggatcg
    23281 acattaatat aagggtccag tttttgattg gtgactttca gaccccggct ctccagcaga
    23341 gcggagatag acgcggctgc cagacctttt cccagtgaag aaagaacgcc gccagtgatg
    23401 aagatgaatt ttgtatgttt cacaatgccc cttcctgatg tgtttatgcg aaggttaaat
    23461 gtccgatggc taagccaagt tttttaaaat aacccctctt attttaaggg cgcttgtatt
    23521 ttccaaaagg ccctgggcag cgatacgatc aagaacatag atcaactcgc cgcctttttg
    23581 ggaataagcc tgaccgtacg atatggggat ttctggcgtc atgtttccca ggattgaccg
    23641 cgtaacggat tccgttttgc gtgcgccgtt ggcgaccaga acgacggttt ttgccttgta
    23701 aaccagttca gcccccatgc tgattgcata ttgcggcgaa tccgccttgg ttttaaaatg
    23761 cccgtcggcc accgcgttgg cgatggtgtt gtcgtccagt ttgaccagca gcatggaact
    23821 gccttgaaaa ggaatgcccg attcgtgaaa ggccacatgg ccgcgaccgc ccacaccgat
    23881 aatctgcaga tcgattcctc cggctgcttt tatttttctg acgtatccgt cgagaatgtc
    23941 tttccggatc caggcaagat aaggtgattg cgcttttgcc ttgatcgcaa tggatttccc
    24001 ggcgtccgtt cccttgaatt cccagtcgct tttgttgtcc ttcaattccc ggatgagcat
    24061 tttctggtcg atcagggaac cgtaaggaac acgggtttct ataaactttt tattgagatg
    24121 accgaaaagt tcctgaatca taaagtaggc atagctttcc ggatgcataa cgcgctgctg
    24181 aatattgttc cccggtaagc cgacatattc atcgagatta aaactgcgaa tgcggccggc
    24241 atccaatcgg ccttcattga ccgctttggc caaatgctta taaaggccgg tcggtgaatt
    24301 tcccgtggcc aatcccagag cgatctcttt tttcctttta agcagttcga tggatttttt
    24361 aataacgatg cctgaagcaa cgtcactcat gtgcgcgaag tctttggtta cccaaatcgt
    24421 tgttcccatt ctgtttatcc ttccccctta tccgatttcg cttttgatga tatccgcaag
    24481 ctgccggcaa tatttcagcg tgacggcatc ggtcggacct tcgaccatca cgcggcacat
    24541 gttctgcgtt ccggaatacc tgaccaggac acgcccttcg tcacccagct ctttttccac
    24601 ctgtttgatg gcttccgcca gcctgggaat ttgatcgata tccggcttgc ttttaacatc
    24661 aacattgatc aacttttgcg gaaaaatgtc catcatgcgc gccagttcgg aaagaggctt
    24721 gttttcttta atcatggcgg ctactagctg cagggctgca ataattccgt cgcctgtggt
    24781 gtgatgatcc agaaaaatca tgtgtccggc ttcttctccg ccgatcacgg atcccatctt
    24841 cagcatatct tcaaggacat aacggtctcc cactttagac gcatgatgct tgaatccgta
    24901 ttgcttgcag gcgaccttca gacccagatt gctcatgacg gtgctgacca gaagatcgtt
    24961 ttttaatcgt ccttcccgct ttaagacgtt ggcgcagatg accagaatct gatctcccgt
    25021 tatttcctgg cctttttcat ccacggcaat caggcgatcg ccgtccccgt caaacgccaa
    25081 gccgatggcc gctccgtttt ccagcacggt ctttttcaga cgttccgtgt gctgggaacc
    25141 gcactggtcg ttgatattca aaccgtttgg cgtcttgtga atgacgtcga tatccgcgcc
    25201 cagttcgtgg aacacatccg gcgcgacccg ataggttgcc ccgttggcgg tgtccagaac
    25261 aattttcatg ccttccaaag acagattgcg gggaaatgtg ttttttgcaa aaacgatgta
    25321 ccgtccgttg acgccttcca ggcggaaggc ccggccgata tcgtcgcctt tggaaaggaa
    25381 ttccgaaagc ttgtcgttta ggatcagatc ttcgatgatg tcttcttttt cgtcggtcag
    25441 tttatagccg tctcctccga atattttcag tccattgtcc tgaaaaggat tatgggaggc
    25501 ggaaatgacc acacccgcat cggcccgcat actctgtgtg acaaaggcga ttcccggtgt
    25561 cggcatgacg cccaccagat aggaataccc tcccatcgag gtaatacctg cctccaggga
    25621 gctttccagc atataccccg aaagcctggt gtctttcccg atgatgataa cagaacggtg
    25681 tcctcccttc ttaaatacat gcgcgatagc ctgtcccact ccaaaagcaa tttgcggtgt
    25741 catgggatgc cgattagctt ctccacgtat cccgtctgtc ccgaataatt tacccattgt
    25801 tctactcctt atctttagat atctcgcgtc gaacaaattg attgttaaaa tgtttttaat
    25861 aaacccagct tgggtacggc tctggatacg ttttcaccaa gatctcgtac gatgccgtcc
    25921 agccacatga cgcctttcga tgaaattccg gctggcagcg cctggattgt ttcaatataa
    25981 tttgttttgt ttttccccgc aatgctttcc agatcgttga gaaacgtgtc acgcagctcg
    26041 tcggtttttc ccgaggcggg ggacgctttc ccgttgcaga ggtcttcaat atgtcccatc
    26101 ttttcatgaa gctcttttct gccccgcgca caggttcgtt caggattttt ttcttcttct
    26161 gctgcggcgg cggctttttg ggccatagcc tttaatcgtt ttaatcgttc ctttcttgct
    26221 gaagaaagtt gctccatcac cccggcgtat aaaaaggaac cgtattcgcc ggtttccaga
    26281 aaaggtttcc tcacatgcgt gtaccaggct tcaagcgctt tcaaatttgc aaggtagagg
    26341 atattgtttt ccacaatccg ttccaggtgg ggataggccg ccgggtaaaa cggtttcaca
    26401 ctgcaggccg gcgctttctc aaagatgagt tgattgtctc cggggtagtc gtgccgcagg
    26461 acggaaccgg cggcgacgac atttccgaat ccaagtctca ccggaccgat gattccgccc
    26521 tggccaccca gaaaaatcgg cggttgattg agcatgacgc cccgcggaac gtcgcccagc
    26581 agagaaggcg ttgttttatc ggcatccggt gtaaaattaa aatgaatgta ggaactgccc
    26641 acttctgaat gattgcttcg gctggtgccg ccgcccatca ggcaatcgca gaagttgatc
    26701 agactgccca gcgttacgaa cgggaaaaga atggtctgct tcaagccgac acaatgcgca
    26761 ccaccggctt cttcttccag aatgcagcct tcccggacgt gcgcgcccat tcccatattt
    26821 gatttttcca gaaagacgga tttgctgaag aaccctccct ttaattcaac cttttcggcc
    26881 agttgacagt tttcaatggt aacgggcgct tcgtatccca gttgacagcc ggatgatatg
    26941 gtcgtttcct ttccgtagat ccggcaaccg ggatatatct tgaccccctt acccgaaatc
    27001 cggtcgatag cgacgtcatc gccgatatcg atggtttcgg gattgggaat gctcacaccg
    27061 cgttcgagca gatcatttat ttttttgagc gatctcggtt ttaaatccat acggtctccc
    27121 tgacgttaag gacggaagat gcctcagcat ccgcgttcgt taaaccacgc cgtaagccag
    27181 catggcattg gcgactttgg taaatccggc aatattggcc ccggtcacat agtcgccctt
    27241 cttcccgtag gtttctgccg tgtcgtagca atttttgtga atgctcctca taatgatgag
    27301 caggcggttg tcgacttctt cacgcgtcca taacagcctg aggctgttct ggctcatttc
    27361 caggccggac gtggcgacac cgcccgcatt ggccgctttt ccgggaccgt aaaggatttt
    27421 tttatcctgg aaaattttga tggcttcagg cgttgagggc atgttggctc cttcggcaac
    27481 acagagacag ccgtttttca ccagtgccgc cgcgttctta ccgttgattt cattttgcgt
    27541 ggcgcagggc agggcgatgt ccactttgat gccctgttcg cgaatgacgt cccagacatt
    27601 tttgccttcg aagcattccg atttgaattt gtttgcgtat tcggaaatgc gcccgcgttt
    27661 gacgtttttc agatcaagca cgaagctgca tttcttgtca tcaatgccgg cttcatcaat
    27721 aatacacgac gaagaatcac acagagagat gaccttgccg cccagttggt tgactttttc
    27781 aaccgcatac tgagccacgt tgccggcgcc gctgacggcg acggtttttc ctttgaaatc
    27841 cattttgcgg gtggccagca tttccgcggc aaaatacaca cagccgtaac ccgtcgcttc
    27901 gggacgaatc agacttccgc cgtatgacat tcctttgccg gtcagcacgc cggtgtgctc
    27961 gttgcggatt tttttgtaat agccgtacat aaagccgatt tcacgaccgc cgactccgat
    28021 gtcacctgcg ggtacgtcca cgtcggcgcc gatgtgacgg tacaattcgc gcataaatgc
    28081 ctgacagaaa cgcatgactt ctccgtcgga ttttcctttg gggtcgaaat cggaaccgcc
    28141 tttggcgccg cccatgggca gggtggtcag tgcatttttg aaaatctgtt caaaccccag
    28201 aaatttgatg atgcccagat tcacggacgg atgaaaacgg ataccgcctt tgaaggggcc
    28261 gatggcgttg ttgaattgca cgcggaatcc gcgattgacc tgaacgtttc ccttgtcgtc
    28321 cacccaggga acccggaaaa tgaccgctct ttccggctcg acaatccttt cgtaaaggtt
    28381 ggctttgacg aactcgggat gctttttgac ggttggttcc agcgtctcca taacttcttc
    28441 aacagcctgg tgaaattccg gctgattggg atcagtctgc ttgactttgg cgatcacatc
    28501 ttgaataact gacataaatt atcatcctcc taaaaaattg ttattgattt cattttttga
    28561 cgttcatgat gcaacgtttt tcatatatct tcaacccgct gaagcgtttc atactccatg
    28621 acggtggaca tcgctttatt ttctatgtcg aacccttctt cgccggctgc ggcaatcggg
    28681 ttgagtcctc caagaagaat catgcccact ttgttgatat ccacattcgt ctggcaaacc
    28741 ggatttccaa cattaccgat cgaaagggcg ccgttaacgc cgatcttttt aaaatcagca
    28801 acgatgtttt ccaccagaga gagcgccaat gccgggattt ctcgaaaatt tgccagaatc
    28861 tttccttccc cctttttgac aacgtccttg acggaggtca ttttgccgcg gataaaaatt
    28921 tcggacggat caagcgacga gccggaataa ttgatgagct ccacgaaacg caaaggatta
    28981 ccgtgccgca cctgcaagat gccgccgaat ttggaatcca tcggaatgcc gtgcttcaga
    29041 agaacgccgt taatgagaat gctgcaaacg gtggcaaatc cgattttccc ctcgggaatg
    29101 gtgacttctc ccagcgtttc accggcagac gcaacagcta caagatccga cacggcaatt
    29161 ttctttttga aaaccggttt catgacggcc aaggccttgc ggaattgttt ttgagggaac
    29221 agagaaatat taacgggaac aaggccgcgc cgttttttta tgtcgaaagt cgtttttaag
    29281 gaaagaacat caatccggga aatggaaaga ctgattttgt cccggacgcg ggcattgttg
    29341 atttcttcca tccccatgtc tgtgatgatt ctgccgtcac gccgcccgat gagcctggtc
    29401 aagcctcttt catccatcga cttcaagtga tagcggacag ttctttcgct taaatgaacg
    29461 ccgtgttctt tcatttttcg cgcgatgacg cgggctcctg cggattctga attttcgttg
    29521 agaatcttga gaatcagaag gacttttctt tctatgtctt cgggattgaa taaatgtttc
    29581 atggctaatc ggcaaccgtt tgccgtatat ctactttcaa atgatgtcct tgtcaataaa
    29641 aaataatatt tttttaaacg gattttcaca ggaacccgat gcgcagcata tcaaccgtaa
    29701 caaatggtta ttttctgata tattgttcag aatttcaata tgttgtgtgg taattattgc
    29761 tatgaaagaa gtgggtttgt ggggtttgtg tttcttcggt ccgacgtgat gtcgtccgga
    29821 ccgggagtac aatttcgtcc ctatctggtc aagatgtgat cctgtgccat tactttcgtt
    29881 gagctccaac tattctgtcg tatgatgacg ggctactttt tgtgttttga ggtggttaaa
    29941 aaaagcgtcc gtcaaaagat gaacgtgttt cggtatgttt taatcggggc gggaaggcac
    30001 attgcagatt ttacttggac tttttacaat caatacccag tttggtcatc ttatctgtaa
    30061 aggtgttgcg gttaatgccc aggaaaattg ccgcggaagt tttaacgccg tttgaccggc
    30121 gtaaagcaat cttaaacaat cccttttcca cgatattcat gacctcttca taaagcttgc
    30181 ttttgcgatc accggacgca gacaaaatgg tggcaatatc ctcaagcttt ttttccataa
    30241 tttcttcaac cgcggcataa ggcgtggcct taacgttcat gacaatttca gatttgctct
    30301 ttttcttcga cggcatttta aacgcatgct ccttccggac gatagtaata tcaactcgtt
    30361 ttaaaatatc gaatgaatat caaattaaaa cgaaagtaat ttcaagacga taaaagcgga
    30421 aataatgagc gctgtgatcc agcaaataac aatcagcgaa cgtcgttccc gcggatgctc
    30481 caggtaattt ctgacctcca tctccaccct ttcggcttcc agcttatcca caatggattg
    30541 aataagtaaa gcgtggtttt catacatgtt ggaaaagaaa taaaaccctt tggtggtggt
    30601 taacagaaaa taagctttgt tcaatatagc aacaacgccc agatgcgtga catccggcca
    30661 cgtcagcgtc ttgacacggg aaaatttttt taccttcatc ccttcattgc ttgcggtaat
    30721 atgtctgttg gctgcttcta tgcccaccag cagggtaacc gcaaaggcaa cggccaaaat
    30781 aattttttcc cacggttgcc cgttcaacag gttcaggatc aacagcacaa aaagcaggat
    30841 gataattgca ataaagggca aaagaaaagc ttttttaatt ttaaaaacgt tctgctccat
    30901 ggtttcgctt cctgtttgat tcgtttcagc acaggctgga cagttgctcg ggttttattt
    30961 gcttctgatg aatgttccgc ttttgccgcc gcttttttcc agcagcatga tgtcgctgat
    31021 gacgattttt ttattcaccg ctttacacat atcgtaaatg gtcagtgcgg cgacgcttac
    31081 ggccgtcagg gcttccattt ccacgccggt ttttcccgct atccggaccg atgcctcgat
    31141 gataatcgcc gattttttct catcaacggt gaacgtcagg tcgacccccg ttaattccag
    31201 agggtgacac atcgggataa gatcccccgt tttttttgcg gccatgatgc cggcgatttt
    31261 tgccgttgtc aaaacattgc ctttggcaat ccgccctttg gatattaacg tcagcgttgc
    31321 tgcatccatc ctgacgattc cccgcgcttt tgcttcacgc gcggtctgag gcttggccgt
    31381 aacatcgacc atttgaacat tgccttcatg attaatatgt gataattttt ttgacattgt
    31441 tgcgaaatat ttttttaacg taattatttt ggtaaagcct taacataggc ctgctgatga
    31501 gtcaagaaac gtagcaacat tttttaaatg cttgaagttg aaatagcttg atataaatag
    31561 gcgaatattt taagtgaaac attcgtggga aaacgggatt atcataatct tgagagtatt
    31621 gaccatgaaa atttcataaa aaacgggatg aagtgtattg gccgcattgc gtgtgcgtgt
    31681 aaaagattcc accgataaac acaaaatatt atcattatta aacaaattgc atcccgtgtc
    31741 ctgcgataag ccgttgattc gcctgggagc cttcggtgac ggcggttacc ttgtgccgga
    31801 tgatttgacg ggaatcgaag cgtgtttttc cccgggggtg ggtttttcat ccgtatttga
    31861 aaaagattgt gcggacaggg ggatgaaagt ttttatggca gacggatttg tgaatcatcc
    31921 aagtctgtct catgagcttt ttgtttttat aaaaaaatat gtcggcaata ccgacaatga
    31981 tcatgaaatg accctggacg cctgggtaaa ttcatccctg gctgatgcca cgggagatct
    32041 cttgctacaa atggatatcg aaggtgccga atatgacgtg ttgtcggcgg tgtcggagga
    32101 tttgttaaaa cggttcagga tcatcgttat tgaatttcat ttgctggacg gactgcgacg
    32161 ccaaccattt tttaatctgg catcgcaggt gtttgaaaaa attttacgga cgcatacttg
    32221 cgtgcacaat catccgaata attgttctga cgccgtgaaa gtggacgaca ttgacatccc
    32281 tatggttatg gaactgacgt tcttacggaa tgatcgtatc cacaatccgt cgtttgtcag
    32341 gacgtttccg catcctttgg attgtgataa tacggacaga aatccctctc ttgttctacc
    32401 gaaaagctgg tatagaaatc atcagaaata gccggtcgcg tttcaggcac cgtttcgaca
    32461 aaacagtgtg aaatcgttga acaagaaaat ctcttgatat aaatatttct attggttagt
    32521 attcgcccca aaaaatataa aaaggcgtta tgttcgttaa agtcaacagc atgagcgtca
    32581 tcgggatgga atcgtatccg gtcttcgtcg aggtcgatac atcccaggga ttgccgcagt
    32641 ttgcaacggt cgggctgccg gatgcgtccg tcaaagaaag ccgcgaccgc atcaaagccg
    32701 ccatcaaaaa ttccgggtat cattttcccc gcagccacgt gaccatcaat ttggctcccg
    32761 ccgatattaa aaaggagggg accggttttg atctgccgat tgccgtgggg atcctggcgg
    32821 ccgagaaatt aatccgtgaa gaaagactgc gcgattgtct ctttgtcgga gaactttctc
    32881 tggacggcag cattaaacgg gtgaacggtc ttctgtctgt tgcgtttaaa gcattggaaa
    32941 ccggcttaac atcggttttt gttcctgaag aaaatgcccc ggaagcggcg atggtgaagg
    33001 gggtgaacat tatcccggtc aaaacgcttc cggatgttgt tgaatgtctg gaaggacgaa
    33061 aaaccataga gccgctttgc ctcgatttgg agaaaatttt tcacaaaaca aatcaatacg
    33121 atgtggattt cagtgaaatt aaaggacaga aacaggccaa aagagcactg gagatagcgg
    33181 cggccggagg ccataacgtc ctgatgctgg ggcctccggg ttccggcaag agcatgctcg
    33241 cgaaaaggct gcccacgata ttgccggatc tctcttttga ggaagccatc gacgttacca
    33301 aagtattttc cgttgcgggt tttttggata aagataaaac gctgatggcg gtgcgccctt
    33361 ttcgttcgcc gcatcatacg atatctgatg ccgggcttgt gggcggagga caaatgccgc
    33421 gtccgggaga aattagcctt gctcatctcg gggtgctgtt tctggatgaa ctgcccgagt
    33481 tcagaaaaaa tgttctggaa tctctccgcc agccgctgga agacggcatg ataactatta
    33541 cgcgttcctc ggtgaccgct tcttttcccg ctaaattcat gctggtggcg gcgatgaatc
    33601 cctgtccctg cggttatttc ggcgaccgcg tccattcctg ccgctgcact ccccagcaga
    33661 tccgtcagta tcagtccaaa atatccggtc cgcttctgga ccgaatagat ttgcacatcg
    33721 aagtgccttc ggtcaaatat cgtgcgctgt ccgacaagga aacgggcgaa tcgtctgcgg
    33781 cgatcaagca gcgcgtgagc aatgcgcgga atatacaaaa tgagcgtttc ggcacaaacg
    33841 gaatcatgtg caatgcgcgg atgacggaaa agcacatccg tcaattctgt caggtcgatg
    33901 aggaagcgca caaactgatt gacatggcca tggaaaaact gggcttaagc gcccgcgcgg
    33961 tcaacaggat actgaaagtg tcccgcacta ttgccgatct ggaacatgac gaatcgatca
    34021 gaacgtctca cgttgccgaa gcgattcagt atcgaaacct tgatagagga ttgatttagt
    34081 gctatgtcaa aaaaagacta tcacaaccag agcggcgtcc tgatgaaatg attgtccggc
    34141 cggcagactg gagaaagagg tatttatgga gatcaaaata aaaaaagtac cgctgaaaaa
    34201 aagaaaaacc aagcccgctg atgaatcaaa actcggtttc ggtaaaatat tcacggacca
    34261 tatgtttacc atgaaatata gagaggggga gggatggtac gatcccgtca tcgaaccgta
    34321 tcatccttta cagcttgatc ccacggccat gtgcctgcat tacggtcagg aaatatttga
    34381 gggactgaag gcctatcggg gacaggacag tgccatttat cttttccgtc cgatggaaaa
    34441 tattaagagg atgaatgcat ccgccgaaag actgtgtatg gcccgaatcg atgaagatct
    34501 ttttatggaa gccatgaaaa aactggtgct tctggaaaaa gactggattc ctcacggtga
    34561 aggaacgtcc atgtatatcc gtccgaccat gattgcgacg gaagcggccc tgggcgttca
    34621 tccggccaat gagtatttgt tcttcattgt agtcggcccg gtgggcgcct attatcctca
    34681 gggattttcg ccgacaaaaa tgtatgtttc ggaggaatat gttcgcgcgg tgcgcggcgg
    34741 tatcggtcat gtcaaggccg ccggcaatta tgccgccagt ctttacgcca gcagaatcgc
    34801 aacaaacatg gggtacacgc aggtgctttg gctggactcc gtggagaaaa aatacgtgga
    34861 ggaggtcggg accagtaaca tatttttcgt gatcggcgac gaattgatta cgcctccgct
    34921 tggaggatcg attctacccg gtgtgacgcg taattcagtc attacgctgg ccagatatct
    34981 ggggacaaac gttgtcgaaa gaccgatatc gattgaagag gttatcaaaa cctcggaaaa
    35041 cggttcatta aaagaagcat ttgcttccgg aacggcggcg gtcatttcgc cggtcggaaa
    35101 tatttacttc cgcgataagg agtatgcgat aaacggcggg aaaaccggtg cgttggccga
    35161 aaaattatat aatgaaatcc tcaaaattca atacggcatg gtggaggacc ccttcggctg
    35221 gcgaattaaa atcgcgaacg gaaaaaaacg ttcccggtaa taattttctg tggacaaaat
    35281 ttgtaaacgc gcttagtcca caagactgtg gaaatcgttg tggacaactt tgttgataaa
    35341 ctctctaagt gcgtaattgt tacgatttta caccaaattg cccaaggttt gagcaattta
    35401 aatatcaagt aaaatcaata tcttattaat attcccttta ggtatcgcca tttaagaagt
    35461 atttatgaaa caagatataa actctgcgaa aaaaaatgtg ccagaaaaga tcgatttttt
    35521 acaaaaaatg atgaaatttt ttcttaatag aaataagcta tttacatcgt catggattgc
    35581 tctgagcgtt attgtcgtgg caatttccat acctggcagc tctttttccg aagaacgtcc
    35641 ggccattgac gatgttgtac aaaaattgca gctgtcctat gagtcaacaa aagatctcag
    35701 cgcggatttt attcaggaga caacggtcaa gtcgatcaaa aagacacagc gggaagaagg
    35761 tcgcgttttc tttaaaaatc cccaaaatat gtcatggaat tatacaaagc cacaggggaa
    35821 aaaactggtg atgaatcatc agacggcctg gctctatctt ccgcaggaaa aagttgccta
    35881 ccgacagagc gccgaaagca tttttcaatc ccgactattg atcgattttt tttccggatc
    35941 gggaaaactc aaagatgatt tcatcatcac atatgacagc gccatatctc cggacaaaga
    36001 cggtcattat ctcctcgtgc ttacgccgcg tcaaaaaaat acgacgttca atgtgttgag
    36061 gcttacaatt gataaaaaca atttttatat cttgcggtta agttttgatg acgctttcgg
    36121 taattcaacg gatctgagat tttcccgtat tcaattcaac accggacttc cgaagacgtt
    36181 tttcctcttc aagccaccca aaggcgtaca ggtttttgaa atgccgtgaa aacccggggc
    36241 gatgagcaaa tttccctttg tcgggttaaa cattaaggtt tggcgatagt cacccacagg
    36301 aaagtttcac cgcctatact gcggtattga aatgcgtcta ttttaaaccc gatggtttcc
    36361 aggattcccc tcagtttttc aggtgtcgtg tggctgtaga acagccgttc tcccataaat
    36421 ttcctgtaac cgctaaagct gtccgctccg gtatacgctt ttgtggcgta ggtaaaaagt
    36481 cccctgccgc ccggtttaag ccagcggtaa attttccgga aaatgccttc gtgcttttcg
    36541 tattcaatgt ggaagagaca ataaatggtt gttaccgcat caaattgttc gccgggaaac
    36601 tccacctccc ggatgtctgc ggcgatggtc ttcattgccg gttggtaacg gtgggccaat
    36661 tccagcattt ttgatgaaaa gtccactccg gtaacctgcc attccttttg aatgaaatag
    36721 gcgggaaacg gttcacccgc accgcaaccc agatcgagca aactgccttt gatggaaggc
    36781 agtcttctga agaaatcgtc catgacatcc gtcatatcga acaggtaacg gtttttgtcg
    36841 taagtttggg caaaacgatc gtaaatttca cgtatatcaa tgtccataaa ttcttttcag
    36901 gatttgaatg gattatccag gctaaaatcc ggtgtcaggt cgaagtcgct gacttcttga
    36961 ataaacaggt tatgaaaacc caatttctcg gcatagtcca aaacctgttc gtactctttt
    37021 tttgtaattc ggcgttttaa atccggatga ttgcggacct tggccatggg cgtatattga
    37081 gacatcaggc ttaaaaaaac attgttggat atttttctgc tgatcaatct caaaactgct
    37141 ttggaattct cgatgcgccc gggcagaacc agatgacgaa tgatgatgcc tttccgcgct
    37201 atcccgtttt ttaattccaa cgcgtcgccg acctggcgca ccatttcttt tatcgagtcc
    37261 gcggcaaacc acggatatcc gggggcggcg gaaaacatca tgctgtcttc ctcctggccg
    37321 tatttgaaat ccggcagata aatgtccacc atgccgtcca gccgtttcag gatgtccggg
    37381 ctttcataac caccgcaatt atacacaaaa ggaattttca atcctgcctt gcgtgcaatc
    37441 aacagcgcct ccatcaccag cggcatctgt gacgtgggcg tgaccggttc tacattgtgg
    37501 cattgccgtt tctccagatc gagcatcaca tcggccagtt gccttgaatt catttcctgc
    37561 cctgtgatgt ggtgggaaat ctggtgattc tgacaataaa tgcagcccag gttgcaggag
    37621 gaaagaaaaa tcgttcccgc gccgtgcgtg ccggagagcg gcggttcttc gccgtgatgg
    37681 gccaacgcgc aatccatgat aatactggcc gcaagaccgc aatatccccg ttcgtttttc
    37741 gtccggttca catgacagtt tcgcggacaa aggatacagt ctttgaaaat ttcctgcagg
    37801 aggaaaattt tttcttccaa attcattctt aaaaattccg tccggagcat cctttggtta
    37861 aaagggttcc accacatcgc ggagtctgcc gctttctacc agttccagaa agtcgtcgcc
    37921 cagcggagcg gacaggtcca ccgctttgca ccaatttctg attcttgttt gcgggtcgtc
    37981 aatgaatgtg accagatccg tgcggtcggg gacttttccg ccgggaaggc tttccgtaaa
    38041 atattgagaa ggaaagacca ttaaaacatt attcagtata ttgggatcgg gcgcacggcg
    38101 cgttattttt ttgtccagcc atcccggaat gatcctttcc tgatgatgaa aaaaaagcac
    38161 gatgtcgttt tctccggacg caaacggatg ggtcagatga taatcgatga ggccgccatc
    38221 gcggtacacg ccgcggggag cgccaaaaat gtcgcggaca ccttcaatca ccagcgggat
    38281 tgcgcccgaa gccagcacag cgtatttgaa attgatttcg ttcagcggga catagatgcc
    38341 gcggaattgc ggtttcaggc aaaaagccgg cggtttggac gcattgtaaa aaacgactct
    38401 ttcggaaaat ttataaatat tttctctact taaatagttc aatagaaaac agaaaatcag
    38461 tcctgttttc tgcaataaga cgttttgaaa agcgacaagg ccgcgtgaac gcgctgtaat
    38521 gatggtcagc cggtatttct tgttgttcag ggcaaaaggc agggcgtcat tttccagata
    38581 cgcattgatg atgccggtta ttttttccag cgtcgttgcc ggtgtatctt tttttgtgta
    38641 ggtaacatga atgtaagcct ccatcagttg ccggtaggat ttgacggctt ccggttgcag
    38701 ccaggcagca aaacggaagg cgcccgctga agagcccacg agctggacag gggtcgaacg
    38761 gcccagagaa catctttcaa ggagtgtcaa atcgaatccg ctggcaatca gccagcgcgg
    38821 tccaaccgcc ggtccgaaat acgtcgaaat cagatcaaag ttgaacccgc cgtctttgat
    38881 gagttgataa attttcctgc cggctttaat tcgtattcta ctcatagatc ttatgtccgg
    38941 gcaaaggcct tgacacgtaa acttttgtct tgtagattca cttacagctc ttatgaattc
    39001 ttatgtgaga agagggttca taatttctat tatgtctttt aagtggggca cgttttatca
    39061 gtaattggtc aaaatgcaag aaaatttatc gacgacacag gccgtgcgtc cggactgttt
    39121 aaaatggctc aaacagacat gggaaaatgg ggcaatgtct gccggacacc cggtgtcatg
    39181 agatggaaga tttcgcaatg ggcagcatca gtgatgatct gtccggaaca tcatcatgat
    39241 gacggcccga cgttctttac tcggattttt tcaagtccgg cgtgagagtt tggaaaggat
    39301 tccgaaaaac actgggcgta aaaaataccg tttgttattg gacaacattt ccggttccgc
    39361 ttccggaatc cggaaattca tttcacgtga ggatgattat catggatata aaagattctg
    39421 ttgagcacat tgcccggaat gcggcgctgg ccgcaaaaaa actttcccgt gtgactgaaa
    39481 cggaaaaaaa cacggctctt ttacgcatgg cggaagcctt ggaacggaat tctaaatttc
    39541 ttctggatga aaacgccaaa gatgtaaacg gcgcgaaaaa agcaggcatc tccaaagcca
    39601 tgctggaacg tctgaccctt aaacaatcaa cgatcgatca aatggccaaa ggattgaggg
    39661 aagttgctac gctgcccgat cctgtcggta aaatcatttc catgtggcgc cggcccaatg
    39721 gtttgctggt gggaaaaatg cgcattcccc tgggagtgat cggcattatt tacgaatcgc
    39781 ggccgaacgt tacggtggac gcttcagcgc tctgcctgaa agcgggcaat gccgtcattt
    39841 tacgcggcgg ttccgagtcg atcaaatcca atctggcgat tgcatccgtt ttgcggaaag
    39901 tattacagga tacatccatt cccgtggaag cgattcaggt cattccggtg acggaccgcg
    39961 aagcggtggc tgccatgctg aaaatggaag aatacatcga tttaatcatt ccccgcggcg
    40021 gggaagatct gatccgcgcg gtggttgccc agtcaagaat ccctgtcatc aagcactata
    40081 agggggtctg tcatattttt gtggatgcca gcgccgatat ggccatggcc gttgatattt
    40141 gtttgaacgc aaagacacag cgccccggcg tgtgcaacgc gatggaaacg ttgcttgtgc
    40201 atcagacgat cgcagggaaa tttcttcccg tggttgccaa taaaatgaaa aaagccggcg
    40261 tcgtcatccg gggatgcgaa aaaacaaaat cgattttgaa acgaatcgaa aaggcggttg
    40321 aagatgactg gtctgccgaa tatctggacc tggttttggc tgtgaaggta gtggaaaaca
    40381 tgaatgaagc cattgcccat attgaaaaat acggctctct gcataccgaa tcgatcatta
    40441 cgggtgatta ttccaatgcc cagcgctttt taaacgatgt caattcttcg accgtcctag
    40501 tgaatgcgtc gacccgtttc agcgacggct ttgaactggg actgggcgcc gaaatcggaa
    40561 tctcgacgac aaaacttcat gctttcggcc ccatgggctt ggaagaactg acgacaacaa
    40621 aatttatcat ttacgggaac ggacaggtga gaacatgaga tggggacttt tcggcggcac
    40681 tttcgatccg attcatttcg gccacttgcg cggggcggag gaaatgctcg aaattttcaa
    40741 tctgaatcgt atcatctttg ttccatcctc tagaccgccg cacaagctgg aagcggatat
    40801 cactgcgttc aatcatcgcg agcagatgat tcgtttggct attgaagaca atgtcaattt
    40861 ctctttttcc gaggtggaaa atctgcgcgc cggtaaatcc tattccgtgg aaacagtcga
    40921 atacatactt aataaataca tggaagatct ggaactttat tttattgtcg ggcaggatgc
    40981 gtttcaggcg gtgacaacct ggaaggattg ggaaaggctt ttgctattat gtaattttgc
    41041 cgtgatgacc cgtcccggtt atgatgatat gaggcttgat gcaattctgc cgagaacctt
    41101 tgccgcaaaa ttcatctacg ataaaaaaac cgacggattt aaaggtccaa cgggacaggc
    41161 catctatttt cgccatacga gtttcctgga tatttcgtcg tcgcgcatga gagacatggt
    41221 cagaaccggc aattccatcc gatatcttac acccggcaag gtccgtcaat acatcatgaa
    41281 gcataatttg tacaaggagt gccgggacaa ttgaacgtgt aaaatttttt taaggagaaa
    41341 aatatgaatt ataaatttat tgaactggag gtcaaggatc atatcgcaac cgtaaccctg
    41401 gccagagaaa aaagcttaaa tgccgttagc ctggaacttg cctcggaaat tacggatgtg
    41461 tttagaaaat taagtgccat ggatgacgtg agagttgtca ttttaaaaag cagggccaga
    41521 attttttgtg ccggtctgga tttgaaggat gccgccggtg gttttacggg aacaaccaga
    41581 gcgccatggg atatgattaa aaaatcacag ccgttgtttg attgctgcaa tgtcattgaa
    41641 gaatgtccca aaccggtcat tgccgctgtg catggtcagt gcattggagc cggacttcac
    41701 attatttccg cctgcgatat ccgcctctgt actcaggatg cgacgttctg cctgaaggaa
    41761 ccgaaaatcg gcttgtgcgc cgacatgggc gcgcttcaac gattgcctct gatcgtgggg
    41821 cagggctata ccagagaaat cgcgttcacc gttgcctttt ataatgccca gcaggttgag
    41881 aaaatggggc tggtcaatca tgtctatgct gatcaggcgg cgctttatga ggcggcccgt
    41941 gaaatggctc gacaaatcac agagaacgct ccgctggcta ttgaatgcac gaaggaggtt
    42001 cttaatttca gcagaggtac gagcgtcagc gaaggaatgt ctctggccat tcaaaaaaac
    42061 atgttcctgc tcgccacaga tgacctgaaa gaagcctttg ttgctttcat ggaaaaaaga
    42121 cagccggatt tcaagggcag ataaagagtc acccccattg acataatgac caaaaaatag
    42181 ggggacggtc cacgatgacc gtccccctat gtcttttttt aagacttccg cgatgttaat
    42241 ctttgtacat gctttcaata tcgtccttga acttttcaaa aacgattctg cgttttaact
    42301 tcagcgtggg agtcaattcg cccgtttcga cgctgaaggg cttatcgagt agtttgtaat
    42361 atttgatctg ctcgtagcgg ggcagtgctt cgttgacttc atcaacctgt ttctgaatca
    42421 gttctttgac ctgttcgttg gcaagcaggt ctttatcgga acacgccggg atgccgttgt
    42481 gtttggaaaa acgatgcagt tcctgaaaat cgggaacgac cagtgcggaa atgtattttc
    42541 tcgcatctcc gatcaaacag acctgatcga tatacggttt ggcggtaagg gccagttcga
    42601 tcggatgagg tgcaatattc ttgcctcctg atgtgacaaa cagttccttt ttgcggtcgg
    42661 taattgtaag aaatccgtcc ttgtcaaatt ctccgatgtc gcccgttttg aaccatccgt
    42721 catccgtgaa agcttccttg gttgcgtccg gcagattcca ataaccttgg aagacctgag
    42781 gtcccttgac aagaatttcg ccgtcctctg cgattttttc ttccgtccac aacgccggtc
    42841 ttccgacggt gccgggttta atctgcaatt tgtgggcgag cgtgttgtat gccagagaaa
    42901 gactcaagga gcggagagga ttggtatagg gagaaagccc tttggcctgc ttggtgacca
    42961 tcaaatcaac cgttaaatca agcatctttt tttgaaacca actggccttg ctgatatcaa
    43021 agctggtggc gaattccggt tcgttgaaat tgatgacggg cgacgtttcg gtcagtccgt
    43081 aaccttcggc cagctgaatg cccagagccc ggataaaaac gcaaacttct ttggatagct
    43141 tgccgccacc cgatatgccg agaacaacat gatccaatcc ggctgttttt ttgattttat
    43201 tgaaaaccag aatgttcgcc agcgcgaact ggagcagatc caagaaaccc agtctggtcg
    43261 ctgtagccat cgcttcgcag tatttttcac ccagccgggt ggcccagttg aaaatggatt
    43321 ggacccgttt atcctgacgg gacacaatcg accgggtcgt atcataaatc ttttcccata
    43381 atcggggaat gctgattaaa acattcggtc tgacttcgag aatattcttt gggatttttt
    43441 caaagctttc agcaaaaacc agaatcccgc tttgctgcag agcacccaca tgataatcgc
    43501 ttgtccggcc gaatacgtga cacatcggca ggtgaaccag gaaagtcagg tgaagtccca
    43561 gttcacgctg tttccgcatc agcgtggaat tggaagcctg aagcatgttg gatacccagt
    43621 ttttctgggt cagaatggcg cctttctgtt ttcccgttgt gcccgatgta taaatgatgg
    43681 aagtgacgtc ttccggagtg acgctgttga tatttttttc tatttcttcg tcgctgattt
    43741 tccctttgcc taagtcgata acttcatcaa acgtgtaaac cccttcgggt tttgcgcctt
    43801 cccaggagca catgacgatg attttttcaa tgggaacccc ctgtgctttt gctttgagag
    43861 ccatctctcc gcggccctgt gtggacgcaa tgacaatttt ggatccgctg tccgatgtca
    43921 tgtagcccag ctcttccacg gttaacgtcg gataaagggg aacgccgatg gcgcaacagg
    43981 cctgaatggc ctgatcggca ataatccatc tgggtctgct ttccgaaaaa ataacgatac
    44041 ggtcgctttt ttttagcccc aggaccatga gccccttggc gaggccgatg gcttcctccc
    44101 gggtctcttt ccatgtccgg gaatgccatt gagcgctggg attgccgttt tgatcaaact
    44161 tggcccgaag cagttcaacc tgatcaccgt aacgcaatgc ctgattgtga aaaatgccgc
    44221 ataaagtatc cgtcctgtat gccataacta catcctcccc cctgtaaagt atttaatgaa
    44281 ctatcgtgat ggtgttggta gatagttttc ttgtttatta atttttgatt tgcagtgcgt
    44341 tttcatacgc ttttttttag tcaaaaatca agtttaaatt ttcggatctt caggaatatc
    44401 gaaggcggcg tttttctttt tataaagaaa acgctctaaa aaacgatgat acacggtcca
    44461 atcggaatcg ggactggagg actcgttcat gaaggtctga aaagcattga atttggcgtc
    44521 cagatcgtcg atataatgga cgacgagcgc ttccagagtc ttcggacgct tgggagaacc
    44581 gaattcgaat tcgccgtggt ggctgagaat gatgtgtcgc atttccattg ccagctgcgg
    44641 cggaaaaccg ttgattgccg cgattttctg atcgatcatt tccacaccca taacgatgtg
    44701 gccgatcaat cttccttcgt cgctgtagtc aataatgctc tcgaaagaga attcgtaaat
    44761 tttgccgatg tcatgaagaa tgcctccggt gatcagcata tccctgtcga ggatggaata
    44821 atgatcggca actttatcca gcaggcggac caccgaaagc gtatgttcca gaagtcctcc
    44881 caggtaggta tggtggaatc ctttggccgc gggcgctcgc cgaaaacggg ctgcagtttc
    44941 ctcttcctgc agaaaagcat tcagcaatgc cttgagatgc ctatggttta ctttttccgc
    45001 aaaggccagc agttccgcaa acattgtttt gacgtcggtc tttaccgcag gcagataatc
    45061 ggcaggatca atatcttgcc ggggaatgtt cttgatgttg agaatggata tttgcagcga
    45121 gtttttgtag cttgcggccc gtccttcgat aaaaatgaca tcgccttttt tgaagacgtt
    45181 atcgaattcc aaggcgtttt cccatacttt acattccatt tcgccggttt tatctttcag
    45241 cctcaccgtc agataggggg agcctttttg tgaaaaggcc atgttttttt ccgttaccag
    45301 aaaagaagaa gcaactttat cgccctgttt gatatccttg agataaacgt ttttatggtt
    45361 caaatgatga cccgcctttc gtgagagtat atgctcatcc gtcctttcct gacatagccg
    45421 atcagcgtta tattggcctg ttttgccagg gtcacggcat tggccgttgg tgcggaatga
    45481 gagatgatga acggtacgcc catgttccat atcttataga ctatttccga agaaattctg
    45541 cccgtggtca atatgatggc ccgatggaga ttgagtttat tcagcagagc atatccgccc
    45601 agcatgtcga tggtattgtg ccggccgata tcttcgcgca gagccgcaat tttttttcta
    45661 aaggccaggg ccgagcagtg cgtgccgcca gtttcattat gcatcgccga accgtttaaa
    45721 agatcgtcca taagtttcag tgccagcgct gtgttgatac tcaagccggc cgggacgttc
    45781 agcggcgtca gcagagcgac gttgcttcgg ccgcgtgcgc cgctcgatgc aatatttttc
    45841 acggacacgt ccggaaaaaa atttttgttc ttcaaaagaa cgtcaacccg gtttttggca
    45901 agggagatgt tttcaatgtc ctgccggcgg gtgatgatct tttccgatcg taagaatccg
    45961 gcggcaagtt cttcgagatg aatgccggcg caggcaatat tggccagggg ggttttgttg
    46021 acccggacgg tgatcatcac ttcctggatc actttgacgc gggatccgga cacggcagaa
    46081 tagctgccgg aaaagcatct tatcgtcttg atgatttttt gcaaatcgtt catgtttttt
    46141 cccttaatac gcggtgatct cctacccgaa ccgtcagttt attcatgtca aagtattcca
    46201 ttagtggaat gatatatttg cgggaaagac ctgtcagttc cttaaaactg gccggtgttg
    46261 ccttgccgtc tttaatcagc attgccttat aatcattgcg cagtttgctc aggatttcgc
    46321 cggaaaagca tagttcttca ttgatcttga tcaattctcc ttctttgagc atcaggctga
    46381 caatgttttg tgctttgttt ttcctgtcaa taaatttgtt gatgacgtct gtcagcgaag
    46441 gaggcatcag tcctgctgtt ttgtatattc cgataatatt ctgacgcaga gcatccagat
    46501 cacccgcaag ctccacctga tgtttggcca gacgcacatt atctttgttg ctgacgatcg
    46561 tctctttttt gttgagcgtg ttgagaacca tgttaaagag tttcggtgaa atatcattgg
    46621 caagggatcc cttcagctct tctttgggaa tgccttcttt caggggattt ttcttgtgat
    46681 acccactgag attataaatg atcatctctt cgagtcgatt gaaaaaacgt gcggaaataa
    46741 ccgtcgtgtc ctcgctatca agcagaaacg cctccttttt ggaaaataaa tcgtccagaa
    46801 cctctcgtat ttttctggat ttcattccca gacggaaggc caattgttga agcggggttc
    46861 ctttaaaccc ggtcctgtcc agaatgacgg taattttttc cggtaaggtg cctttttgca
    46921 gcaacatcag ttcttcgatc gctccgcggt tgaaccgttt atgtttttgc ggaagcggat
    46981 cgataatgac gccgccgccg atggtggtaa ccggggaata actgcgcaag acaaaatgat
    47041 cgccggcgac aacaacgtct ttttcagaaa gaatgagttg ggcgtatcct tgctgcccgg
    47101 gggcaagttc atctgtatcc agaagaataa tccgggaaat gatttcgttc gtgccggtgt
    47161 gcagccgaac cagcgcgcgg tttttaagac ttttggtatt ggcggaaaga tagtcgacgg
    47221 cgatatccag ccgctgcgta ggccagacgg tgtgcggccg caccagaaca ttgcctcgca
    47281 cgatggcgga tttttcaata ccttgcaggt tgaccgctgt acgctgtccg gcccaggctt
    47341 cttcctcagg ccggttatgt acctgaatgc ctctgatgcg ggcggaaata ttctggggaa
    47401 gaatctgtac gtcttcccct gttttaaggt gatcggaaac cagggtaccg gtgacgattg
    47461 tgccgaaacc cttcatggta aaaatgcgat ccacgggcag acggaaaata ccgtcgtccg
    47521 ttcttgcggt aatctctgcg atgatggtat ccagggcctg aagtagctcg gacaacccct
    47581 cctgttttat ggcggaaacg ggaatgaccg gtgcgctttc cagaaatgtt cctcgaagaa
    47641 aatcggtgat ttcagactcg acaagattaa gccagtcttt ttccacgaga tcaatcttgg
    47701 tcagggctac gattcccctg gagattccca gaagggaaca gatgtgcaga tgttctcttg
    47761 tttgaggcat gatgccttca tctgcggcaa tgaccattaa aaccagatca atgccggcag
    47821 cgccggaaac catgtgtttc acaaactttt cgtgtccggg aacatccact atcccgagtg
    47881 tcagacccga aggcaatttc agggaagcaa aaccgagttc gatggtgatg cccctttgtt
    47941 tttcctcttt gagacggtct gtgtcaattc ctgtaagagc tttgattaaa gccgttttcc
    48001 cgtgatcgac atgaccggcg gtgcccaaaa caaaatgctt cattttttaa tcctgattat
    48061 ctgaaagtga aaaataacaa actcttcatt ttttaacaag gttatatttc aagacatcaa
    48121 aaataaaaaa cccatgttga aaaaaactgg acaaattaat tcttgaaaat gccggatgga
    48181 attgttagaa aaaaataaag aggtattttt tgcgtatact tggtcttgat tacggcgaaa
    48241 aacgaatagg ggcggccatc tgtgatgaac tggggttgac agcccaggga ttgtccacaa
    48301 tcatacgaaa aacaaaaaag catgacgggg aaattttaga gaggctcatc agggatttta
    48361 acgttgagaa aatcgtcatc gggtatcccc tgaagctgga cggttcggaa ggcatccagt
    48421 gcgaaaaagt taatcgtttt gccgcgctgc tggaaaaaac cttcggtttg ccggtgatca
    48481 ggtggccgga aacgctgtcc accaaagaag ccgaagaaat actgatcaac tccggtatcc
    48541 ggtggggaaa aagaaaaaaa atggtcgata aactggcggc ctgtcttatt cttcaaagtt
    48601 atctggattc tgtcgcccca tcattaaaaa ataatttctg aacaaccatg aaaaatcctg
    48661 ataaaaagaa tatcctgaaa aagtcaaatg aagaaaacaa cgcggtcgat caaatgacgt
    48721 cacaggcaag tgcttggaaa agtaaattcc atgaaatctt catttttctg aaaaaaatat
    48781 tttccggaag aagatggatt cgccgcactg ttttatacag tgttgccgtc atcctcatat
    48841 tctgtattat gttattggat tatgcattca gtccgatcga tgtccgagat aaaacggttg
    48901 tgattgatat cccgacaggt tccagcttca tggaagcgac tgaattgctt cagaaagaag
    48961 gattaatcac aaaccgtttt ttcttttaca gcctggccgc gatcaagagg gccagtcgta
    49021 acattcgtgc cggagagtat gaaatcaata cgttgacgaa ccccataaca atgattaaca
    49081 aactcatgca cggtgatata aaagtctatg aggttgtgat tccggaagat ttgtcatttc
    49141 gcgacatcgc ggtgattctg gacaagcata agctgattaa taaagaaata ttttttgagc
    49201 tggccaggga tcaggaattc ttaaaatcgt tgaatattga agcggagtct atcgaagggt
    49261 atctgtttcc tgatacgtat cattttaacc gttcgatgaa tacacgtcgc atcatgaaga
    49321 aaatggtcga tacattctgg cagaaaatga cgccgtccat gattcggagg gcgaatcaga
    49381 tgggattaag cgtgcataaa ttagtgacca tggcctccat catcggcaaa gagtccggta
    49441 gtgctctcga gaaaccttcc gttgccgctg tattttacaa caggttgaaa agaggaatgc
    49501 ctttgcaaag cgatccgacg gccgtttatg acatggatga ctttgagggt aaaatttttc
    49561 gtagtcacct gaagaggaat tctccatata atacctatcg gatcaaaggg ttacctccag
    49621 ggccgattgc caatcccggc ttggattcgc taaaggcaac gcttttcccg agctctgtcg
    49681 attatcttta ttttgtttcc aaaaaagacg gcactcacca tttttccagt tcattggtgg
    49741 aacataatca ggcggttaat cggtatatta atttaaaaga tgaacaaaat caattacttg
    49801 aatatgatat tcccgttgag gagtattaaa ataaaaaatc cagcctttgt ttaaaaattt
    49861 cctgtttttt tgtttttctc gccataaaat atttttttat cagaatttcc ctatttcttc
    49921 agtaatttta agctatttaa tttaaaaaaa agttgcattt tttacttgac aaagacaaat
    49981 agcgcctcta tagttgccac gtggagtaaa gtggcttatc gtggagggaa gtgatgtctg
    50041 ttttcagggg gcagtatcac catacaattg atgaaaaggg aaggataatc tttccgtcaa
    50101 agtttcgtga aatctttgct gaagagtatg acaaccggat ggtgattacc aactgggatc
    50161 agtatctcat ggtctttcct tacgatgaat ggctggttat tgaagagaag gtcgcccatc
    50221 aatccaagtt gcgcaaggaa gtcagatcgt ttcagcgttt ctttatgtcc ggagcgattg
    50281 attgcagtat tgacggacag gggcgtgtgc tgattccgcc gtctttgcgc gaatatgctc
    50341 aattggaaaa agatattgtt ctggccggca tgctgaaaaa tattgaaatc tggtcgaagg
    50401 accgtttcga agaaaggatg aagcaggctg tcggagaaat tgacaaatgc gccgacttta
    50461 tagctgatct gggaatttaa accatgacgg ccgagtttta tcaccagccg gtcatggtga
    50521 aggaagttat agattcgctt atcgtcagca gggccggggt ttatgtggat ggcaccgttg
    50581 gaggggctgg ccattcatac gcgatcctca agcagacgga tgctttgctg atcggcattg
    50641 attgcgatga tgaagctctg cggtttgccg aaaaaagact gcaagaattc ggctcacgca
    50701 aaattttact aaaagaaaat tttgttaact tagaccatat actgaaaagt ttaaacatag
    50761 aaaaagttga cggtgtgctt ctggatctgg gtgtttcatc ccgtcaactg gatgcatctg
    50821 aacgaggttt cagcttcagt cagcaggcgc cgctcgatat gcgcatggat cgcagcatga
    50881 cgttgagcgc ttatgatatt gtcaacagct tcgcgcaaaa cgaactggaa aaaattatta
    50941 ggttttacgg agaagaaaaa atggcttcaa gaatagccag ggcaatatca aaaaaaagac
    51001 aggaatcgcc gattgcaaca acgacacagc tggcggcgat tgtcgcatcc tgcatgcccg
    51061 ccggaacgcg atggcgcaaa attcatcccg cgaccagaac gttccaggcg atcagaattg
    51121 ccgtgaatca cgagttggac aacatcaaac cggccatgca tgcggcagtt gatgtgttaa
    51181 agaccggcgg ccgtctttgc atgatctctt ttcattctct ggaagaccgt attgtcaaaa
    51241 atgagattcg ttcgctggaa gggcgttgcg tatgcccccc cgacagtccg ttttgtgtct
    51301 gtcagagaga tgccaaactt aaaaatctga caaaaaaagc gatcatgcct tcggaggaag
    51361 aaatagcgtc caatccgcgg gcgagaagcg ctaaattaag agtagccggg agattgtaag
    51421 atggccccat ccgctcaatc agcagacgca cgagttgtcc cccatctggg agaagtcgaa
    51481 ggagcgtccg tcggaattaa atatccgacg tttattgttc tcgccgttgt gctgatgctt
    51541 gtgtttgttg cttatgtcgg ctcgcatatt cgcatgaccg aactggaata caatatcgca
    51601 gcggaaatca gtacccagga gaaaattctg gaagaacaga aaaaattgaa actggaatat
    51661 gccatgctca aatcgccgca acgaatagaa aatcttgtcc gcgacagatt gcagatgtcc
    51721 taccctgaca atgaacaggt cattgttata agaaaatcgg gaggctgaca tggcgattga
    51781 atcgaaaaaa agaatgaaat tcagaatcat catgattttc atgttttttt tcgttttatt
    51841 tgtggcgctc atatcgcgcg cgtttcagct tcaggtttta tccggtcaaa agctgaagaa
    51901 actggcaaca aggcagcata cgacggtttt gcagttgcag ccggaacggg gaattattta
    51961 cgaccgcaac ggtgaaaaat tggccatttc cattatggcc gattccgtct gtgccgatcc
    52021 gtcgaaagtc attgatccgg ccaaggcatc caaacagatt gcgtacatcc tcgggttgga
    52081 cagtcaggcg gtgtataaaa aattatccgc ccccagaaac ttctgctggc tggccaggcg
    52141 gatttcgcct gagcaaaccg ctgcagtggt gaaagcggat gtcgagggcg tttttctggt
    52201 caaggagccg aaacggtttt atcccaatgg agagttggcc gcccatctgc tgggttttgt
    52261 cggattggat gcttccggtc tggaagggtt ggaaaagaaa tacaatgact ctttagtggg
    52321 cagcccggaa aaacttgaat gggcgaggga tgccaaaggg aagaaacttt tcctccgggt
    52381 ggaaaaaacg cagaccgcaa aaaatgaaaa taccaacctt gtcctcacga tcgacaaccg
    52441 aatccaatat cttgttgaga cccatttaaa agaggcggtg attgaaaaag gcgccaaggg
    52501 agggctggct attgtcatgg atccgaagac gggagagatt ctggcgctgg ccaatcagat
    52561 cgggtttaat ccgaacaaca tccagggact gtctcccgag cgatggcgga atcgcgccat
    52621 taccgattat tatgatccgg ggtccacgtt taaaccgttt ttggtggccg ccgctctgga
    52681 agaaaaagtt attcgcgaac aggacagatt tttttgtgaa aacggcaatt acaggattgc
    52741 cgatcgcgtg attcatgagg ccaacagaaa acgttacggc acattaacgg tcagggagat
    52801 cttaaaatat tccagtaaca tcggttccgc aaagattgcc cagaaaatgg gcaaagaaaa
    52861 attttataat tatattcaaa aattcggttt cggatccaaa accggaatcg atttgccggg
    52921 ggaatcttcc ggactggtgc ggccggtgag ggactgggta aaagttgata catccatgat
    52981 tgctttcggt cagggtattt ccgttacggc cattcagttg atcagtgccg tctccgcaat
    53041 cgcgaacaac ggcgttttga tgaaaccgta tatcgtgcgc ggcattgccg accaggataa
    53101 ccggttggtc aaactctata cacccaccgt ggtgcgtcag gtcatttctc ccagagtggc
    53161 caagagcgtg acgaatatgc tgatggatgt cgttggtgcc gaagacggca ccggtaaaaa
    53221 tgcgaagatt gtcaatgtgg cggtagccgg caaaacagga acatcccaga aattcgacac
    53281 cgcccgcggc gtctactcat cggaaaaagt tcgtacgtcg tttatgggat ttttcccggc
    53341 taaagatcct caggttgcca tactggtggt tctcgatgag ccgcaaaaag acaaatgggg
    53401 tggcgttgcg tcggcgcccg tattcaaaaa tatagcggaa cagattctga actgttttaa
    53461 aaccgatatc agagaaaccc ccgtttttaa gaaggaaaaa accgataagg tgcagttggt
    53521 gtcggcggaa caaaccttgc tccctgaaat tgtcgaaaac agcgacggac cgtccatgcc
    53581 caattttatg ggattaacga tcagggaagc gttgaaaaaa gcaaaaaccg aatccatcga
    53641 gctgaaaata tcgggcaacg gctgggccgt gcgtcagtac cccgaaccgc acgcgcctct
    53701 gggccatgaa cgtgtatgca gcgtggtttt tgaattgacg gattgaggat ggcatgaaac
    53761 tgactcggtt gacggaagga ttggatatga tcaatatgtc gggtgaggcg tccggagacg
    53821 tgtccggtgt ttgtttttct gcggaccagt gcgtgccgga gtctctgttt gttgccattc
    53881 cgggattaaa acgtgacggg cacgatttca ttgaggaagc catatcccgc ggcgcgagat
    53941 tcattgttca tgagaaggtt ctgacgttcc cgccattcgt aagagcgatc aaggtgtccg
    54001 acagccgccg ggcgctgggg atcctggcca agaattattt cggtgatcct tccgcggcgc
    54061 tgaccgtaat tgcggtgatg ggcaccaacg gcaaaacaac catctcctat ctgctggaat
    54121 ccatcttaaa aggagccgga tgcaaatgcg gtgtcctggg caccgtcaat tatcgctata
    54181 acgacaaggt ctatgatgct cccaacacta cgccggaatc ttacgagatg cagaagatcc
    54241 tccgcgccat ggccgacgac ggcgtcaccc atgttgtagc cgaggtttca tcgcatgccg
    54301 ttgatttgaa gagggtggac gattgtgatt ttgatctggg tatttttacc aatttgaccc
    54361 gcgaccatct ggattatcac gtcacgatgg aaaattattt tcaggcgaag aaaagatttt
    54421 ttgcggaaat ccttccgcaa agcagaaaag gttatgcgca gaaaatgatc atcaacgccg
    54481 atgacgactg gggacggaga attctgaagg acgtgcccct gccggcactg acttacggaa
    54541 tagaaaacga ctgtgccgta cgatcgttaa actgcaagct gtctctgaac ggcatagaag
    54601 cggacatccg cttggcggga aacaatgtct tgatccaatc acccctcatc ggcaaattca
    54661 atctgtacaa tattctagcc gctgccggag cggcttttat tttacacatt cccgtgccgg
    54721 tgattaaagc ggggatcgaa aatttatcgc atgttcctgg gagattggaa tcggtgcctt
    54781 ccgccggagg ggttcacgtt tttgtcgatt atgcccatac ggatgatgcg ctgcggcgtg
    54841 ttcttcaaaa tttatccgag cttaaaaaaa acaaaatcat aaccgttttt ggttgcggcg
    54901 gcaacaggga caaaggcaaa agggccctga tgggagacgc ggccacccgg tacagcgatt
    54961 tgacgatcat tacctcggac aatccgcgtt tggaaaatcc gctggatatc atcgctgata
    55021 tagaagcggg cattgaccgg aaagacgtta aaaaaagcga tccggaaatg ttaaaaatga
    55081 acggacgtgc tcgttcatat gcagtgatcc ccgaacggag aaaggcgatt gaggcggcga
    55141 tcagggccgc ccggagagac gatatcgttt tgattgcagg caaaggccat gaagattatc
    55201 agattctcgg gacggagaag atatcgtttg atgaccgggt tgttgcacgg cagattcttg
    55261 aaaccaagag ttattgagac gaacaatgaa gagtgagtca cctgtatttt ttttgaatga
    55321 cgtgctgaca tcaacgtccg ggacgatgat gtccggattg ccggaaactg tttttcgcgg
    55381 cgtttccaca gattcccgtt cgataaaaaa aggcaatctt tttgtcgccc tgaaggggga
    55441 tcattttgac ggtcacgatt atgccgagtc ggtttttcag ggaggcgctg ccggaatact
    55501 ggcgcaggat gatgaaaaaa tacgccggat gaaagtggat gatacctttg cggtaataaa
    55561 ggttgccgat acattattcg cgctgggaga gatggcgcat gactggagaa agaaattttc
    55621 cattccggtg atcggattga ccggatcttc gggaaagacg acggccaagg aaatgatagc
    55681 gtccatcctc gggttgaaaa aaaatatcct gaagacggaa gggaatctga acaacctgat
    55741 cggattgccg cagacgatat tccgcctgac ggatcagcat gacatgatgg ttctcgagat
    55801 ggggacgaat acccggggag aaattagacg actgacccgc attgccgaac cggatatcgg
    55861 attgattaca aacgtgggac cggctcatct ggaagggttt gggtccctgc aggttgtcgg
    55921 ggaagagaaa ggggatttat ttctgaatat gccatcgtcg ggaatcattg tggtcaatct
    55981 ggatgatgaa gccatcctga cggctgctga gacgtggaaa ggcaggaaga tcacgttcag
    56041 catgagcccc aatgcggatg tgaccgtctt tgatatcgaa agaaacggtg cgaaaggagt
    56101 tcgtttcaac cttgctatcc ggggcaatac gcagaaagtg gaaatgaaaa taaccggact
    56161 tcatcacatc aataacgcga tggccgctgc ggcggtggca gtggcagcgg ggatggatct
    56221 gaaaaatatt gcggaaggtc tctcggcgtt ccgtccgttt cacggacgga tggaaattat
    56281 caaactccgc aacggcgcgt atcttctgga tgattcctac aatgccaatc ccgcatcggt
    56341 ccgggaagcc ctgatgaccc tgaaagattt aaagggcaat caccatgcgt acgtttttct
    56401 gggagacatg ctggaactgg gcgcacaagc cgatgaaatg caccggaaga taggaatgct
    56461 gattgccacc atcggtgtga agacgctgtt tctccagggt gactatgccg gaatgaccgc
    56521 tgccggcgcg acggaaggag gcatggcgtc cgacagcatc ctctttttgt ccgataagga
    56581 aaacggtact gattttctga aaaaatgcct gaaaaaaggg gactggattc tggtgaaagg
    56641 ttcgcgccgc atgaacatgg acagaattgc ggcgcagatc tgtgaagatt tcggcagaga
    56701 taaagtcagc gaggagaaca agacagacta ttaatcagga gtaaagaaga ttgatttacc
    56761 atttactgta tccgctgcat accaccattt cgttctttaa cgtttttcgt tatatcacgt
    56821 tcaggacgat ctttgccgcc attacggcgc tgctgatttg tctgattttt gggaaatgga
    56881 tgattcggaa gcttcaggac atgcagatcg gtcagcagat cagggatgac gggccgcaaa
    56941 gtcatctggt gaagaaaggc actcccacca tgggaggcat gttaattatt atggcggtcg
    57001 tcgtatccac gttgctctgg acgaatctgg cggacgtgta tgtctggctg atactcatgg
    57061 tgacgcttgg cttcggtttg atcggtttta tcgacgatta cagcaaactg gccggcaaga
    57121 gttcccgggg agtctccgga aaaacgagac tggcggccga gattatcatt gctctgtttg
    57181 tcagtgtgat tctttatgtg aagccaggtt ttaacgcgca ggtaacgatt ccttttttca
    57241 aaacggtatt gcctgatctt ggttggggct atgttctgct ttcgaccttt attattgtcg
    57301 gtgcggccaa tgccgtcaat ttgacggatg gcctggacgg tctggccatc ggtccggtcg
    57361 tgatttgttt tgccacctac attctttttg cgtattttgc cggaaatata aagacatcga
    57421 attatctgca gattgttcat gtttccggag caggagaact ggcggtattt gccggggcga
    57481 ttgtcggtgc gggactcgga tttttgtggt ttaacgctta tcccgcggaa gtattcatgg
    57541 gcgatgtcgg atctttgtct ctgggtggcg cgttgggcac gatagcggtc attaccaaac
    57601 aggaaatcct gctggccatt gtcggcggga tatttgttgt ggaaaccttc tcggtcattt
    57661 ttcaggtcgg ttatttcaag atgaccgggg ggaaacggat tttaaaaatg gcgcctctgc
    57721 atcatcactt cgagttgaag ggttgggcgg agccgaaagt cattgtcagg ttctggatca
    57781 tttccatttt gctggcgtta atggccatca gcacattgaa attgcgttag agggacaatg
    57841 gattttaacg gtaaaaaaat tgttgtcatc ggcgtgggaa aaacaggcgt ggccgtcgcc
    57901 cggtttctgg ggaaaaagag cgccagaatc gtggtgactg atgaaaaatc accggaccat
    57961 tgggatggcg gatttaagca aattgccggc gagccatggc tggaagtcgg cgaatataac
    58021 agcgtggatg ttttaaacga cgcggatatg gttattcctt ctcccggcat tcctcccggt
    58081 aatcctttgc tggcggaggc cttgaagaag aacattgccg tcgtcagtga aattgaactg
    58141 gcctaccggt ttatcagggt tccgattatc gccgtaacgg gcaccaacgg taaaacgact
    58201 actaccacct tgctgggtga aattttgcag aaatcgggga agaaagtttt tgtcggcgga
    58261 aatatcggca atccgttgat tgaatacgca gataaaaatc aggatgccga ttttatcgtg
    58321 gctgaaatca gcagttttca actgcagtgg atcgatcaat tccggccctt cattgccgtt
    58381 cttttgaata tcaccagcga tcatgtcaat tatcacgggt cttttgccga gtatcgccgc
    58441 atcaaagcca gaatctttgc caatcagacc ggaaaagatt tcgcgattct gaatgccgaa
    58501 gacgatgaac aaaagaagat cgaacggaac atcagggcgc gtgttgccgg attcagttca
    58561 aagcatgttc tgccctcggg aatatttgtt aaagaagaga atattgtttt gagtctgccg
    58621 gggatgcaag aagaaaaata tcctttgagc atgatcaaca ttcccggctt gcacaatgtg
    58681 gagaatgtga tggcggcgat tttagcggct cgtttctgcg gatgtgatcc gtcggatacg
    58741 atccgggctg ttgaaaactt ccgggggttg cctcaccgca tcgaatttgt ggaagaaaag
    58801 aattccgtca agttcttcga tgattccaag ggcacgaatg tcggatcggt cctgcgcgcg
    58861 ctggaaacgt tcacccagcc ggtgattttg ctgctgggcg gacgggacaa ggacggggat
    58921 ttcgaaaccc tgcagccggt gctggcggcc aaagccaaaa aaatcgtttt attcggcgaa
    58981 gcgcgcgaac gcattgcctc attgatcggc caaactgttc cggtcatcaa aaagacaaaa
    59041 ttgcaggaag ccgtggaagt tgcgtatcag gaagccgtgc ccggcgacgt cgtgctatta
    59101 tcaccgggct gtgccagttt tgatgaattt gccaattata aagaacgagg taatttcttt
    59161 agaaaagtgg tggaaaatct gtaaatggaa accgcgtaca aaaaagaaga caattatccc
    59221 gattttgttc tgctgctggt tacgctgctg ctggcggtgg tgggaaccgc catgatttat
    59281 tcctccagtt ccatcattgc cctggagaag ttcaacgacg gccagtattt tttaaagaaa
    59341 cagctcgtgt ttgtgtttct gggattcacg atcatggtcg cgatgacaaa agttccgtat
    59401 acgcatttaa gaaaaatcgc ctatccggga atgctgctgt gcctgctgtt actgtccctg
    59461 ttgtttgtgc cgggtatcgg catgcgccgg gggggcgcca cccgctggct gaatctggga
    59521 ggcttttctt ttcaggtgac ggaaatggta aaaatcatga tcgtgatttt tttgtcgcat
    59581 ctgctgacac ggaaggcttt tcagataaca aattttacaa agggggtgct tgtcccgctg
    59641 gctgccacgt cggtggtcat gatgctcatt ctgctggaac ctgattttgg aacaacggcg
    59701 attattgcca cgatcatgct gatgatgctg tgtattgcag gggcgcagat caaacatctt
    59761 gcgctgctga tcggggcctt cattcccgtg ggcatcgctc tgatcctgta taaaggatac
    59821 aggctgacgc gactgaccgc ttttctggat ccctggaaag atgcggacaa tacgggtttc
    59881 cagatcattc aatcgctgat ctctttcggt tccggcggga cattcggcgt gggtatcggc
    59941 gacggcatgc agaaactatt ttacctgcct gaaccgcaca cggactttat tctttccatt
    60001 attgccgaag agagcggttt catcggtgtg gcgatggtca tcaccatgtt tgcccttttt
    60061 gcctaccgcg gtttccggat cgcgctgaac gcccccgatc tgttcggaag tttgctggcg
    60121 tccgggctga ccatggtgat ttgcatggaa gcattcatca atattgccgg tgtgatgggg
    60181 cttattcccc tgaagggact ggttctgccg tttttgagct atggcggaac ggcatttatc
    60241 atgagtatga cggccgtggg aatattgctc aatatttcca cgcagacatc gtgagagcgg
    60301 tgtgatgata ttatttaaaa acatgagtgg ctttgtgtgg agcgaacaat gcggattgta
    60361 attgcgggag gaggaacagg cggtcatttg tttcccggga ttgccatcgc ggaggaattt
    60421 ataaaaagaa atgcacagac caaggtggtg ttcatcggaa cgaaaagagg cattgaaagc
    60481 cgattgctca gccgattggg ctatgacctc gagacgattg acattgaggg catcaaggga
    60541 cgggggatca gggcattggt gaaaagtgtt ttccagattc caaaaagttt gtggcaggcc
    60601 agaggaattc tgaaacaatt cagtcctgac atggtggtcg gtgtcggcgg ttacgcatcg
    60661 ggtcctgcgg tgctggcggc gtatctgatg gggatcccga cgtccattgc tgaacaaaac
    60721 gccatccccg gtgtcaccaa caaaattctg ggaaacgttg caggcaaaat atttgtcact
    60781 tacccccgga cacagcaatg cttcccgcgt cgaaaagttg tgatcagcgg caatccggtg
    60841 agggcgtcgt ttgccgcaca gagtttccga acgaaagaag acagaacgca ccggcaactc
    60901 ctgattttcg gcgggtcaca gggcgccgaa gcgatcaaca ggggcgtcat cgatatgttg
    60961 ccgcggttaa gcggcatgaa agaccggata cgcattgtgc atcagaccgg tgcgcggcag
    61021 ttggaagagg ttaaaaaagc ttatacgcaa tccggtcttc aggcggtggt gacggacttc
    61081 atcatggata tggctgaagc ctatgcgcag gcggatttga tcatttgccg cgccggtgcg
    61141 acgtcgctgg cggaaataac ggttgcgggc aaggccgctg ttttgattcc gtatccgtgg
    61201 gcggccaatg atcaccaact gaaaaatgct ctggccatgt ccgacgcggg agccgcggtg
    61261 gtgattccgg aaaaagagct gagtggcgaa agcctgttca acgccattga cggcattctg
    61321 cgtgatgaaa accggcggct attgatggaa gaaaattccc tgaggatggg aaaagcggag
    61381 gcggcggcaa cgatcgttga tgaatgcatg aaactgacgg acgccaaaca ttaaagacag
    61441 agtgcttgga aagcggaaaa acaatgaaaa aagataagga aaaaagcctg atgcagcgca
    61501 aaatcaaatg catccatttt gtcggaatcg gcggaatcgg catgagcggc attgccgagg
    61561 ttctggttaa tctcggatac aatgtgggcg gttcagatgt tcaaccgtcg gaaaccaccg
    61621 cccggctgca gaagctcggc gctaaaatcg tcatcggaca tgcggcggag aacgtcggga
    61681 atgccgatgt tgtggtgacc tcaacggcgg tgaaggagca taaccccgaa gtgctggaag
    61741 cgcaccggaa aaatattccg gtcatcccgc gtgcggaaat gctggcggaa ctgctcaaaa
    61801 tgaaattttc ggtggctgtt tccgggagtc acggcaaaac gacgaccacg tccatgattt
    61861 ccaccatcct ggcggagggg aatctggatc cgaccatggt catcgggggt aaactggcca
    61921 gcatcggcag caatgcccgg ttgggtgacg gcgaaatcat tgtggcggaa gccgatgaaa
    61981 gcgacggttc gtttctgaaa ttgtcgccga ccatcgccgt catcaccaat atcgaccggg
    62041 agcatctgga ttattatccg ggcatcgccg aaatcaagga ggcgtttctg acgtttgcca
    62101 atatcgttcc cttttatggg tgcacggtcc tgtgtaacga caatattcat gtgcgggaga
    62161 ttcttccggc gatcaaacga cggatgatca cctacggtat cgataccccg gcggattaca
    62221 gcgccgagga tataaaattt acaaaaagaa aaacgtgtta cagtctggtt cataagggag
    62281 agaaattggg caagatcgaa ttaattgttc ccggtctgtt caatgtttat aattctctgg
    62341 cggcgatagc ggttgcccgg gagttgaacc tggatctggc caccatcaaa gaaggattga
    62401 aaagtttttc cggggtgcag cgccggttgg agatcaaggg aagggtcggc gaagtaactg
    62461 ttcttgatga ttacggtcat cacccgacgg aaatcaaggc gacgctggcg gccatgcggc
    62521 aaatctggaa ggacagactg atcgtcgttt ttcagccgca cagatataca cggacacggg
    62581 cgctttttga ggagtttacg aaagcatttg acgaggttga cattctgatt ctcaatgaca
    62641 tttatccggc cagtgaagag ccgattccag gtattaccgc tgccgcgctt tgcgaggcga
    62701 tccggaaggc cggtcataag catgtggaat atattccggg tgcggaagcg accgtcgaat
    62761 atctcttaaa aacggttcat cagaaggata cggttgccac catcggagcg ggcagtattt
    62821 ataaaatagg agaaacgttc attactcaac tggtagccag gggaaagaat acgtaatgac
    62881 cagccattgt gagatcaggg gcattttaca ggagatagat gccgggaaag tattgtacga
    62941 cgagccgatg tcgctgcata catcgctggc cgtgggtgga aaggcggatg ccctggttgt
    63001 cgtggagcac gaaaatcaac tggcgcgggt catcaggaaa ttcaggatgg agggcataaa
    63061 ttatttcacg gcgggaaatc tgaccaatgt tattgtcaag gacggcggat accggggagc
    63121 gatcatattg atgacgggta tgaaggaaat tcagtgcaaa cctgtggggg gccatgaagg
    63181 ttatatcctg gcccagacgg gagcctctct ggccaaggtt gtcagccggt cggtcgtgga
    63241 ggcgttgagc ggcgttgagt tctgttccgg cattccgggc agtgtcggcg gagccgtctg
    63301 gatgaacgcg ggagcgtacg gacgggaaat gaaggatgtg attgaatcgg taacggtgct
    63361 cgataccgaa ggcagcaaaa aaacaatgat ccgggaagcg atctctttcg gttatcgcac
    63421 atcgtctttt ccctcgggaa caatcatcct gggggcccgg ttcagactga aaaaaggtga
    63481 tcgcgcgaca atgaaagcga gaatcagcga aattttacaa tggcgccagg caaaacatcc
    63541 cctggaatat ccgagtgccg gatcggtttt taaaaatcct cccggacatc cggctggaaa
    63601 gattattgag gacatgggac tcaaaggtga aaaggcaggc ggcgcggaaa tatcgtccaa
    63661 acacgccaac tttatcgtca ataaaggtgg ggcaacggct gcggatgttc tggcgctgat
    63721 ccgtctggtc cagtccagag ccagagaaga aaaaaatatc gatctggaaa ccgaggttgt
    63781 cgtgatcggg gaagacagat gttaaagaaa acgggcattc aaacaaaaca aaacagaatc
    63841 cgccggcgtt ttacgagctt gtccggagat tttttcacgg cgatgacatt gctgagcatg
    63901 gctgttttcc tcagcgtcct gtttatttat gtttacaatg tgatgatgag cagcccgtat
    63961 tttcagatca aggaaatatc cgtgcgcgga ctgaaggaac tgacggagaa ggatattctg
    64021 gcatcggcga atattaaacc acaacagaat cttctggccg tgaacaaaaa ttcgatcatc
    64081 cgccgtgtct gttccaatca ctgggtggaa acggtttacg tgggcaagga actgccggga
    64141 aaactcgtcc tggacgtccg ggagagaatt cctctggccc tggtaaagcg gggtggtgat
    64201 ttttatctga tggatgtgaa aggtcatgtt tttaaaagac tgggtggaag cgatgaagtt
    64261 gatctgccgg tcattaccgg tgctgcaaca ggcgaagcaa cccgttcgcc gctgctgttg
    64321 agcgcactcg gtttgatcca gaggatttct aaatctcccg cgtatgctca tctcggaacg
    64381 atttccgaga ttcatatcga ttccgtgttc gggcttgcgc tggtgtccga caacggtctg
    64441 tatttgaaac tgggcacgga tgattttgaa aataagctga ggaagttaaa ggccattctg
    64501 accgatttgg aaaacagggg aatgaagaca ggtttcttgt gtgttgattt gtcggatcat
    64561 tccaaagtga cggtcaaacg caaatatgtt ccggaaaaaa cgcaagatgg cgatcagaat
    64621 aaaaattatc tgatctagaa agtataaata aaaaagaggt gaaaaggctg atgggaaaga
    64681 gaagcaatgt tttagttggt cttgatatcg gaacgacaaa aaccacggcg atcgtcggtg
    64741 acgtaaccga aacgggtatc gatatcatcg ggatcggaac atcgcagacc aaagaaatga
    64801 gaaaaggcac ggttgtcaat atcgacacca tggtggagtc catcaagacg gcggtggagg
    64861 aagcggagca tatgtccggg tgccgcatca attcggcatt tgtcgggatt tccggaagcc
    64921 acattaaagg ccagaactct ttaagtatcg tttccattaa gggaagggaa gtggatgaaa
    64981 aagacgtgca gagagtgatt gaagcttccg aggccattgc cattcctgtc ggacggaaaa
    65041 tcctgcatat tcttccgcag agttacgtga ttgacggtca ggaagggatc aaggatccca
    65101 tcggcatgtc cggtgtccgt ctcgaagcca aggttcacat tgtaaccggt gcgattgccg
    65161 ccattcagga tattgaaaaa tcggtgaacc gcgtcggtct ggatattgac ggcattgtcc
    65221 tggagcaact tgccgccggg gaagccgtgc tcagcagcga tgaaaaggaa ctgggggtcg
    65281 tcctcgtgga tatcggaggg tccaatacat ccgtgaccat tttttccgaa tccagcatca
    65341 aacacacggc cattctgccg gtcggcggca attatctgac ctcggatata tccaccggtc
    65401 tgcgtacgcc tctgagcgaa gccgaaaaaa tcaaaatcaa acacggctgt gccatgacat
    65461 ccatgattcc caaggatgag accatccgag tgcccagcct cggcggaagg gaagaccgcg
    65521 aagtctcccg gcagatcctg gggcgtattg tcgaaccccg gatggaagaa attttaaacc
    65581 tggcctacaa cgaagtggtg aaattcggtt ttgaggatct gctggccgcg ggcgtcgttc
    65641 tgaccggggg gacatcgttg ctggagggca tcagcgaact ggctgaaaag atctttgata
    65701 tgccggcgcg caagggcatt cccatcggcg tcggaggatt gaacgacatt gtcaactcac
    65761 cggcgcacgc catcggcgta gggttgatta tctacggcca taaacagaat gcattaagcg
    65821 gagtttcaac agggatgttt ggacatatca caagaactgt gaaaaaaatg ttttcagaat
    65881 ttttctgaaa ggaaggagag cgcatgtttg aattaacgga agtctgtagt gaaaatctgg
    65941 caaaaattaa agtgattggt gtcggcgggg gcggcggcaa tgccgtcaac acgatgatcg
    66001 catcctgcct gcagggtgtt gattttatca acgccaacac ggatgcgcag gccctcggcg
    66061 tgtccagcgc accggtcaaa attcaaatcg gaagtcaaat caccaaaggg ctcggagccg
    66121 gttccgatcc ggagatcggg aaacaatccg ctctggaatc ccgcgatgaa atccgtcctt
    66181 atctggaagg cgcggatatg attttcatca cggcgggcat gggcggagga acgggaacag
    66241 gcgccgctcc ggtgattgca gagattgccc gcgagtgcag cattctgacc atcgccgttg
    66301 tgaccaagcc gttccagttt gaagggaaaa agcgcaatat gcaggcggag gaaggcatcg
    66361 aacagctcag ggaaaaagtg gatacgctga tcgttgttcc gaatcagaga ttgttaagcc
    66421 tgggaggccg caacctttcg cttcttgaag cgtttaagaa ggcggatgac attctttatc
    66481 aggccgtcaa gggcatctcc gacctgattt tagtgccggg cctgattaat ctggattttg
    66541 ccgatgtcag aagcgtcatg agcaatatgg gcatggcgat tatgggtacg ggcatcgcca
    66601 gcggtgaaaa cagagccgtc gaagcggccc agagagcgat ttcgtctccg ttgctggaag
    66661 acaataccat tcagggcgcc cacggtattc tgctcaatat taccggcggg tcggacatga
    66721 gcctctatga agtcaacgag gcctcgtccc tgattcagga agaggcggat gaagacgcca
    66781 atattatctt cggcacggtg attgatcaga acatggaaga tgaaatccgc attaccgtta
    66841 tcgccaccgg ttttgatgat ttcgccagga agaaaaatga ttcagtcggc aacgttaccc
    66901 ggttccgcgg cgtcggcgga cgggaggatc ttgctacccc gacgtttatc cgtcgggaaa
    66961 gaaaactcaa cagcggtgaa atagccgttg tgggtcttgg cgacgaaacg gagaatatgg
    67021 atattcagat tcccacgttt ttaaggcgtc aggctgactg atcatgatgc tttcaccaga
    67081 aacgttatcc gggatgttcc gtttcacggg caggccgtga tcaagccatg gattggaaac
    67141 taaaagcagt atacaaaagc atactggaga atgaaaccgg atatgtaaaa aaaatatggg
    67201 ggaaagacaa caccgtttgc cttgtttatc ccaatcacta caggataggc atggcgaatc
    67261 tggggtttca aaccgtttat caactattaa atgctcaacc ttcctttctt tgtgagaggg
    67321 cattcctgtc cgtttccgga aacgaggcca gatgcgtttc cggaacggcg ggaatcttca
    67381 gcctggaaaa tcaaaaagca ttaaccgaat ttgacatcct ggcgttttcc atctcctttg
    67441 aaaacgatta cccgaatatt ctgaaaatca tggatgatgc cggtatgccg ctacgatccg
    67501 cagatcgctc ggaaaaatat cctctgattc tggggggcgg tattgctccg acgttgaacc
    67561 cggagccgct ggccgatttc tttgatctct ttgttctggg ggaagcagag gacattttgc
    67621 cggtattctg ccgttttttt gaagcatcgc gccgtctggg gcagcgccgg gaacaatttc
    67681 tgaaagatgc gcaaaagcaa ctgcatcatg tttacgttcc ggggctctac gacgttcact
    67741 attcgccgga aagaaaaatc acatcggtgg ttccgcggga agcggggttg ccggaaaaaa
    67801 taaaagtgga gcctgtaaac gatatcaatg cgttccgcac acaggaggtg atcagcgcgt
    67861 cttgcaccga gatggaggac atgttcctgg tcgaggtcaa ccgcggttgc gcacatggct
    67921 gcagattctg tgcggcgagt ttcatttacc gcccggttcg tttccgcggc attgaggaaa
    67981 tgatcggcgc aattcatgac ggcctgacgc gaaaaaataa aatcgggctc gtgggcaccg
    68041 ccgtttccga tcatccggac atccccgccg tctgccggta catcattgcc cataacggcc
    68101 gggcggggct cggttccctg agaatcgacc ggatcaatga ggagatggtc ggtctcataa
    68161 aagcaaacgg catcgagacc gtggcactgg cgccggaagc cggcagtcag aagatgcgcg
    68221 atatcctgaa aaagaaaatc agtgaagagc aaatcatgta tgccacagcc ttactcgcag
    68281 agcatggcat tgccaatata aggctgtact tcatgatcgg ccttccacgg gaagaggagt
    68341 cggacatcga tgccatcatc ggtctgacca aaaaaattca gcaccatgta cgtctccggt
    68401 tcgacgggaa aaaacagttc cggcgcttga cgctgagcat caatcagttt attcccaaac
    68461 cggcaacgcc gtttcaatgg gttcctctgc tcgacgtcca tatcgccggc aaaagaatca
    68521 aaaagatcaa agaggcgttt cgaaaagaca agtctgtccg cgtgattcac gacgtcccga
    68581 aatggaacta tgttcaggca ctgcttgcgc tgggcgaccg gcgtgttgga gaaatactgc
    68641 tggccgtcaa tcgtctggac ggcaactggg gccggggact caaagaagtc aacgtcaatc
    68701 cggattttta cgtttaccgt gagaaatctt tcgatgaaat tctgccctgg gatattattg
    68761 atctgggaat atccagaaaa gcgctgatca acgaaaacca aaaggccttg aaatgacgac
    68821 ggatgatcag gcattgtcct gttttgccgc gattcccgcc gctatttttt cctgttgctg
    68881 ccactggccg tagatgatgc cgttagcaca aagccggttg agttcctttt tcagcgccgg
    68941 accgattttc ccggcggttt cgttttcgaa ggccgtccgg ggcagcggca tgaatgtatg
    69001 agcgtgaatt tttgcgcccg accggctcag gtcttgtata acgttcagcg tggaggcaat
    69061 atcctcgtct gtctccccgg gcaggccgaa tatgaaatca actttcgcct tcaaaccggc
    69121 ctgacgggtg acatccacgg cgttgtatat ggttgccaca tcatattcgc gtccggcgca
    69181 atccagcatt cgctggctgc cggattgcgc cccgatgatc agattgtcgt tgttggcgta
    69241 ctttttcacc atcttgaccg tatgggcatt gacgtgttcc ggcctgactt ctgacggaaa
    69301 actgccgaag aatattttac cgctctttcc gaccaggcgc ctgatgtccg acaacaggtt
    69361 ctccagtttt tcattattga ttcttttgcc gtcttccgag ccgtatgaaa aagcgttggg
    69421 ggaaataaat cgaaaatacg tcgaatcctg tcttttcatt attttgacgc aatccagaat
    69481 attttctata ctgcggtgcc gcggtgttgt gccaaacaac tgcgagacct gacagtaacg
    69541 gcaggcataa ggacagcccc gcgtaatctc gatagggccg tatttggaaa aatagtgcga
    69601 gatgggataa aaagtgttga tgtctgctgc ttcgcgcctg ccggtaaaaa cgtactctcc
    69661 gtttttgtcc aggtatgaca aaccttttat gtccgaatta ttttcatcat gattaatttt
    69721 ggacagtaat tccgtaagcg tttcttctcc ttcgccgctg attacgaaat cgaagcctag
    69781 tttcagcgtc tccagtggag cgcctgcggg atgaggccct cctgccagaa agacggtttc
    69841 gccggctaat tttttcaccc tgttaatcag gtctttgatc ttcaggaact gaggcgtgca
    69901 gaaggagatt ccaatgattg tttctctgtg cttttttgaa atggcggata ttttttgaag
    69961 aagttctttt tcagaggata gaaaatacac gtcgatgttt ttgatgtccg gatacttttc
    70021 cagagccccg gccagcgcgt tgtagctgtt caggtttcct ttgaggtagt aaaaaattaa
    70081 tgccgtttcg ccatccataa atcggaaatt cgtttcatta ttgaggggca tttgtctgtg
    70141 tgagacgttt cgtggcgaag cgcgataccc ctctgacaag gggcatcgtg aaaatcaggt
    70201 tcgactcgtt ttaaaaaccg cgccttgggc aatcgtccgg agatcatgac ggccaggcgt
    70261 ctcaatgctc tctaaatgca cattgagacg cccgaacagc cattgatgac aatccttgcg
    70321 gaagattgcc tgtaaacgtt cattacgcca aatcttcatt gtctgctgtt ttgtaatcgc
    70381 gaaaagattc cacgcctgtc atctcggagt actttcccgc agctttttca tatccttctt
    70441 ttgctttggc cataagttca tccgccttgc gggcaatatc ttcgcgcgat tccttgccgc
    70501 ttttgggtgc aaacaaaatt cccaacgcta cgccgatcag gccgccgaca atcaggcctg
    70561 tgaaaaaatc acttttgctg tctgccataa aacacctcct caaatttaac aaagtccttg
    70621 cagtctgacg gatcatcctc ccaaacacga acctcttgtc agaccggaat taccccgtat
    70681 ttcaatacgc tagttttaat ttagcataaa acaggaaaat tagaaacgga gaccttccga
    70741 cagatctatc cggacatgat tctctttgcg ggtccgggtt gagtacgaaa tgcaagaata
    70801 tttttctatt gtgcatggac gcctcggatt tttattcttc cggccgcaga cgatggtcaa
    70861 ccatgtgagg attgctcaac gtaaattttt gtcgtgaaga gaaaccgcaa agcatcgcac
    70921 cggatgatga atctttcgat taaaatcgac gcatgggaca taaacaaaga gcatcgaatc
    70981 acaagaaaag cacttgtccc ttcacgcagc gtttttttac cagcgttcca aaatttcctt
    71041 ggtgagttcc gaacggatgg cctgatccag atgcacgctg ccgaagaaac ggctgcccgc
    71101 catcttcgcg ttgagcaccg aaaggccgtt ggagcgttcg tcgagccagg gaacatcaat
    71161 ctgatcgtag gagaaatcgc cggtgaaaac catttttgtt ccctcggcgc cgcgcgtcgc
    71221 tatggctttg acatgggatg gcggaaggtt ctgaatctcg tcgacgatga cataacattt
    71281 ttgaatggag cggccgcgaa tcttgtccag atccatcatt tcgatttttc cgttggccag
    71341 ccagtcgttg atcttttctt tcatcctttc gtcaaccagt tctttgatgg cattgatatt
    71401 gtcgtagatc ggatgcatcc agggtttcat ttttgcgtcg atgtctcccg gcgaataacc
    71461 cagccggtcc ttgtttccca gttggatggt ggcacggctg accagaatct gccggtagtg
    71521 cgacgcctgt tcaaaagcgg ccagcagcgc gagaaaggtt ttagcggttc ccgccttacc
    71581 ggtcaacgtc accaggggaa cagttgcatc gcgcagtaaa tccatcgcga taaactggcc
    71641 ccagttgaca tcgccgttat tggaacgcgg cttgatcccc agcaaatgaa tgtcccggtc
    71701 gataatacgg aaatagtcgc ccttgcgtac ggcaacgaag gccggtcccc agacgccatc
    71761 aaggtcgcag taacagatga tgccttcatt atacaggacc tgttccagtt gcgcagccag
    71821 tttggcttcg ggcagtttca agggaaaggt tttatccgaa atccagctcc gttgaagatt
    71881 aatcgtcgcg cgagtggtat ggtgctgctt gatgtctgtc tgatgcattt tgatggattg
    71941 cacatcgaga ccgaaattca gagccagggc ctccaacgcg gcatcctgag tcacgagaat
    72001 gactttggct ttgcccagtt tctttttggc attgagtgcg gtggcaataa tacagtgatc
    72061 atttttatcg accttcagat cggcgtcttt caccagattg aaatcatact gaaaaaaacg
    72121 gacggaatgg tcttttgcgc gaaatagatt acggacattg cgaataacgt tgcgggcaat
    72181 gtccgccttg aaatcgtttc ctgttttatg gctgtccagt tcctcccacg tcgcataagg
    72241 aatcagcaca caattgcggc ctccctggcg caatttttca aaaacatcca ttccgtattc
    72301 cagcgcgctg gtatccacca caaaatactt ttccggaacc ttctctgccg ccttgacggt
    72361 ctccattgcg attgcgtgca cctcagctct tttggcattc gtcatagaat cgctcctggg
    72421 attaatgaac tgtgatttat gaaaggtaga agttctgcgg gcacacctcc tgaaccggac
    72481 cagccggttt cgttattgag ttgataagaa cttcattttc ggcatttcca gtataagaaa
    72541 cgttatcttg tgtgtcaagg atattttgac cacaagatat ggggcaaaac ggcatttttt
    72601 cttcatacgg catcctcctg cagacggaca attccaaaac gccgtctatt ttaagtaaaa
    72661 aaacgatcgt ccattgcttt cattttacag ggaaataaag agatactttt gtacccttgc
    72721 ccggttggct ttcaatttcc attctaccgc cgtgggcaat catgagatgt ttaacgatgg
    72781 ataatcccag acccgtgccg cccaaatcgc gtgaacgtga tttgtcaacg cgataaaaac
    72841 gttcccccag acggctgatg tcgtctttcg gaacgccgat accggaatcc tggatcgata
    72901 caaccgtgta agatcccgat tcagaagcgt taatactaat ctcgccgccg ttcggggtaa
    72961 acttgacggc gttgtccaga atattgacaa agatctgcgt gagcctgtcc cggttcgccg
    73021 taatcggagg gaaattatcg ctgacattat tacgcacgga tatatttttc agttcggcct
    73081 tcgcttcaat caacgacagc atagtgtcga tggcctttct tagagaaacg ctttcaaaat
    73141 tcaaaacggt ttgtttcgtt tcaatattgg aaatcgtcag cagatcttcc acaagccggt
    73201 tgaggcgttc ggcctgtttc aaaatgatgt cgataaactt tttcgtttcc tcctgattgg
    73261 taatcgcgcc ggattgaatg gtttccagat agccgataat tgccgtcagc ggtgttttga
    73321 tttcatgcgt cacgttggcg acaaaatcca tgcggacctg ttccagtttt ttcaggcgtg
    73381 tcacatcgtg aaagacaatc atcgttttct cttcatccgg gcggccgtga acggcggaga
    73441 ggctgacatt cagcacgact ttatccgcat ggccgaggat aatttcacgc gacacgctga
    73501 tgcatttttc cttgaactcc tgaaatgcct tctgcaaatc aatattgcgg aaagcttcca
    73561 tcagtgtttt gccgacgaca tcgtcgtatt gaatcgccag catgttactt aaaaccgggc
    73621 tgctgaattc gatgacgcct tgttcgtcaa gaatcagaac gccttcgttc atgctggtga
    73681 aagccgtcat cagcttgttc ttctcttcgt tggcgatgtt tatcttttcc cgcagttctt
    73741 caaccagata attgatgtta tcggccaggg ttttggtttc atccgacgta tccagaatga
    73801 tggacccgac cggatctccg cgccgtaatt tttcggtaaa ttgttccatg atttttatcg
    73861 gttcgtacaa acgatatgaa aagaacagtg caattcccaa cgcaatcagc gccacgacga
    73921 ataaagtcag gagaatgtac tgatatacct tgtcgataac gttttgtacg tgatgcagcg
    73981 ggcgcgccaa tcttacgtat cccttgatct tctgtgcgtc tttgacggcg acagccatat
    74041 agagcatatc gatcccgaga gaatcgctgt accggacaga tttccctgtg cctctgagcc
    74101 gggcttcctg cacttccggg cggttaaaat gactctccag cagagcaatg tctttttccg
    74161 aatccgcaaa aaccctgccc cgggcgtcca tgaatgtgat tcgcgactgg gagatttggg
    74221 caacctgctt cagatggccg gatgcctttt ccagagacgt ctggtcaatc agttgtgcat
    74281 aggacaataa ttccgtttca atttgtgacg tcagaacttt tctgacttcg tcccgcacaa
    74341 aaatatccag aagaaagaaa gacaggcaga tgatgaccag ataactgaaa aatattttat
    74401 agaacaggtt ttttttcact cgatatgcct ctatttttca tccctggaaa gatgacggac
    74461 gttttcaccc gtgatcatgt acgttaccat atccgcgatc ccctgggcat gatcggcaat
    74521 ccgctccagg ttttttgaaa cgtgcataat taataaagcc cgatgaatgg atttggaatc
    74581 ctccatcata aacgtcaaaa gttcccggaa tatctgctcg ttgagatcgt ccacttcctg
    74641 ttcgatctgc cggacatcat cggcgatctg taaatcccgt ttaatcaggg catccagcgc
    74701 tttagccacc attttctcca caatggacga catattgggc aaatcgatat acggtttgag
    74761 tttgggttct tcgttgagtt gtatggcttt ttctgcgatg ttgaccgcca tgtcgccgat
    74821 tcgttccaga tggccggtga tttttatggc tgtggtgatg aatcgtaaat cgatggctgt
    74881 cggctggcgc agcgccagca tcttaataca tttctcttca atatccttgt ccattttatc
    74941 aatttcataa tcattggcga taactttctt cgccaaatcc gtgtctctgt ccgtcagaga
    75001 tgtcatggct gaatggatgg ctttttccgt cagtgcgccg agataaatca gattgttttt
    75061 tatgtcctgc agctccatct cataatgtga actggtatgt cttctttctt ccatagatga
    75121 tcctccggtt aatgcttagc caaatcttcc tgtaatataa tcttccgtct gtttgacatc
    75181 cggattggta aatatttttt cagttttatt atattcaacc agattaccca gataaaaaaa
    75241 cgccgtttca tcggaaatcc tggctgcctg ctgcatatta tgggtcacga tgatgatagt
    75301 atagtctttt ttcaactctt caatcaactc ttcgattttg gcggtcgaaa tggggtccag
    75361 cgccgacgtc ggttcgtcca tcaaaagcac atccggcttc atggccagcg cccgggcaat
    75421 acagagccgc tgctgctggc ctccggagag attcatggcg gacttattta gaatatcctt
    75481 gacttcatcc cagagaacgg cctgtttgag gctttgttcc accagtgcgg ccatatcgtc
    75541 cacggcgatg ccggtgagtt tcgggccata ggcaatgttg ttgtagatgg ttttcgggaa
    75601 gggattgggc ttctgaaaaa ccatgcctat tttacggcgg atttcaaccg gatccacatc
    75661 aggggcataa atatttttac tttcaaagag gatttctcct tccacctttg tcccgtcaat
    75721 cagatcattc atccggttta aaagcctcag caatgtggac ttgccgcagc cggaagggcc
    75781 gatcagcgcc gtcactttat ttttgacaaa tgtcattgaa atatcggtca gggcgcggct
    75841 ttcgccataa taaaaattca cattgttcaa ttgaataatt ctggaatcca ttcttaccac
    75901 cttttatttt tcctcatata tccgcggatg ataatcgcga tgagatcaat acccaggaca
    75961 acggccacca gaacaagcac tgttccgtac tgaatgggtc gcgttgcctc gatgtttgtt
    76021 ccggccgtcg ccaggacata gatatgatac ggcagcgcca tgacttcgtc aaatatcgaa
    76081 gaaggaagct tggctgtgaa aaaagcggcg gctgtaaaca tgatgggggc cgtttccccc
    76141 gcggcccggc cgatccccag aatggaaccg gtcagaattc ccggcagggc attgggcaaa
    76201 acgattcgat aaattgtctg ccatttggaa acaccgagcg ataaagacgc ttcacgaaac
    76261 gtttgcggca cggctttgag cgcttcttcc gacgcgccga tgatcgtcgg cagaattaaa
    76321 aaacccagcg ttagagaccc ggccagaata cttgagccga actttaaaaa aatgacaaaa
    76381 aaacctaatc cgaatagccc gaacacaacc gagggaacgc cggccaggca attgatgccg
    76441 actttgatca accgcacaac ccggccctgt ctggcatact ccgtcagata gattgccgag
    76501 gcaatgccca gcggcaagcc gatcgcaata gcgcccaggg tcagataaat cgtccccagg
    76561 atagccggca tgataccgcc tttggtcatc gcgtccgtcg gaggcttcgt gataaaatcc
    76621 cagctaatta cagaaatgcc gttgatcagg atgtacgaga tgattccgcc cagcgcaagc
    76681 gttatcatca gagccgacag gcggacgcag gaaaaaaaca atccttgttt aaaatagcgc
    76741 catttcatcg catgggtttg aagtatctgc atcacgttac cttttatagc gttccggatc
    76801 cgacctgttt aaacttgtgc gacacataat cggcaatcaa attaaaagcc agcgtcagac
    76861 agaacagcac aatcccgatg gcaaaaagcg cctgatagtg caggctgcgg aaaggcgtct
    76921 cgcccatttc cgcggcaatg cttgccggca tcggccgcac cgagtcaaaa agactttccg
    76981 gaatggcggc cgctccgccg gcaaccatta acacaaccat cgtttcaccg atggcccggg
    77041 ccatgcccag cataaccgcc gtcgaaatgc cggagagggc ggccggcaga atgaccctgg
    77101 caatggtttc aaattttgtg gcgcccagcg cataagaagc ttccttgaat tcccgcggga
    77161 cggcgtaaag cgcgtcctct gagatactgg atatggtggg aatcgccatc agcgccagaa
    77221 tcagcgatgc attgacaata ttcaaaccgg tcggcaggtc gaaggtttcc tgcatccagg
    77281 gggcgacgat gaccatgccg aaaaatccca gaacaacgga cggcagaccg gccagcagtt
    77341 cgataaccga tttgagcgtg atcttaatat tctgcggcgc gatttcggaa atgtaaatgg
    77401 ccgccagcac acccagagga acagcgatga acgttgataa aatggtgacg atcaatgagc
    77461 cgacgatcag cggaaaaatg ccgaaagacg gcggatcata cgtgggatac cactccatcc
    77521 cgaaaagaaa atcttttata gatatttttc cgaacagcgg gatgccttca cggaaaagag
    77581 aaaatacgat caaacccaga acgaggaccg acaccagtga aaaaataaaa aagatgtatt
    77641 tgatgataat ttctttaatt tgtctggtca taacatttca ctttattgcc tgatggaatt
    77701 ctttttcaac ccacgctttt atttcatcgc ggacacgtct gtattcatga gtaatgtctt
    77761 cagcgttccc attaaatttg gacggatcgg aaaaacgttt atgaatatgt ctatctcctc
    77821 gcgggaagtg gggacacgat tcctgaacct gatcgcaaac cgtgatgacg caatcaaaat
    77881 gcatatccgg gaaaacgctt aaactcttcg actgattggc gctgagatcc atgccggttt
    77941 ccttcatcac cgtgacaacg agaggatcaa tctgtgtggg atcggacccg gcactgaatg
    78001 ccgagtaacg atccccgtaa agatgattca aaaacgcttc agctatctgc gaccgcaccg
    78061 cattatgggt gcataaaaac aatacgtttt tcttcatact gtcattttgc atcaggttcc
    78121 cttttcaagc gtattggcgt cataaaatca ttttattttg tcaacggaac aaatccttct
    78181 ttctggacaa ttttctgccc ttcggcggat ttaacaaagg ctaaatactt ggccacgtcg
    78241 ccggttgcgt taccgtcggt gtacatgtag agctcgcggg aaaaaggata ttgtttggaa
    78301 agcgccgtct ggatggttgc cgtaatgccg ttgacctgca agggggtcac gcttttgtcc
    78361 agatagccga tgccgagata cccgatggca taacgatttt tggcaatcgc cgtaacgatg
    78421 gcgccgttgg aggccagcat ttgcgctctc ggcgtgacct tggttttttt catgacgaaa
    78481 tgatcccatg attcaaatgt gcccgatgaa gaatcgcggg aaatgacaac gatcttcaaa
    78541 tcatcgccgc cgacttcctt ccagttggta attttaccct gataaatctg actgagttga
    78601 tcaaccgata aatttttgac cttgtttctt ggatggacaa cgggaacaat cgcatcatgc
    78661 gcgaccacat gggcaaccgg atttacattt ttggttttgg ccagcgcaat ctcttccttc
    78721 ttgatttctc gggatgatgt ggcgatgtgt gccgtcttat caatcagggc tttgatcccc
    78781 tcgccggaac cgccgcccga aagcgacatc tgcacgtcag gatgcttttt cataaaggct
    78841 tcaagcgtgc tctgggttat cggcaagacg gtggttgatc ccttgataac gatcgatgag
    78901 ccggccagag caactgagct catcagggct acggctgcga tcgttaaggc tgctttttta
    78961 agaatactga acattgaact ttcctcctgc tttttgaatt tgccgttact ctacagaatt
    79021 tatgttacag tttgatgaca gcttaaagaa agtttagaaa aaaacgcaaa aaaagcagac
    79081 ggccgattac ttcaggtcgg caaatttata gccaaagccg cgaacggtaa gaatatattt
    79141 gggattttca atatcctgct cgatctggga acggagtcgc cggatatgca catcaacagc
    79201 gcgatcggtt atgaacgtgt cgtcacccca gacatgatcg ataatctgat tgcgggaata
    79261 gacccggccg ggatggcgcg atagaaagaa gagaagcttc agttccgtcg ggcttaacgt
    79321 aactttttta ccatttacca gaacaagaca cgattcgtaa ttgattttaa gtcccgcgtg
    79381 actgaatttc tcttttccgg cgtatagctc attttctttc aggcggcgca ggacgctgcg
    79441 caccctggcg gtcaattcct tgacgctaaa cggcttggtg acatagtcat cggcgccgac
    79501 ttcgagaccg gtgactttgt ccacctcatc gctttttgcc gtcagcatga tgatcggcag
    79561 acccgacgtt tccggattgc gccggacggt tttacagaca tccagaccgt tcattttcgg
    79621 cagcatcaaa tcaaggataa tcaaatccgg cttttgtgct ctgatgattt ggagggcgga
    79681 ctcgccatcg agagccttga ccgtagaaaa tccctctttc tccagattat aggaaacgag
    79741 atccacgata tctttttcat catcgacaat cagaatctta tacatagtca ttcattgcca
    79801 ttttcgggtg caaaagaaaa ttaaaaatat caaaatatga gagcgggaca aagcatgtgt
    79861 tctttataaa ccgttcataa acgtcatctg cccagagaat caaaaataat ttccgccgga
    79921 tccaaagcgg gcatgccgga aagtccggcc agctgcatcc ggcaggaacc gcaatccgac
    79981 acgatattgt ccatgccggt tttgctgatc agggagacgg cgcggttgcc gatctgtatc
    80041 gccgtaagag cattttcttt tttaaaaccg tagctgccgg aaagtccgca acaggcgtcg
    80101 tcgaggattt catattccag gccggggatc agcgacatga gttttgccgc cggataaccg
    80161 atcttcagag cccggagatg gcagggaata tgataggcga tgctgcgtct gacctcccgg
    80221 aagtcgggtt tgacataccc ttcattgatg agtttgatga tgtactcatg cacgtcataa
    80281 ctgttttcag caatttttct gccctgcgga atttccagaa tgcccgggta atcatggagc
    80341 aggcaaagcc cgcaggaggt gcaggtgtaa atcacgtcgt aaccggcgtc gatgtaattt
    80401 gacagggatt cggcattttt tttcgcgaaa tatcttgcca gcgtcagatt cccgttgccc
    80461 agtgcgggca ggccgcagca ccactggggc ggcagaacaa catcgacgtt catcgaagcc
    80521 agcaggcgga tgatttttct gccgatgtcg gggcggtaaa agttgatgaa acacccgtag
    80581 aaaaaagcga ctttttttct gcccttgttg agtcttttct tttcccggcg cattgtccgg
    80641 aagctgaatt ttggaaaggg cagatacgtc gagatcccca ttttctgaaa aatctttttt
    80701 ccggtctgag ttgacgcaac aacattggcc aggggagcgg tgaaagaccc cagagcgctc
    80761 aaataatagt tattggcaaa cagaagatgc gccggcgttt gcggatgttc gttgccgtat
    80821 cgctgctggg ctttcagaat gatctgtgcc ggctccacac cgcaggggca ggccatttcg
    80881 cagcgcttgc actggctgca cagcagcacc cagccgtcct gcgccttgtc tctttccgag
    80941 cggaaacgct gcgcgtccgg tccggcctgt ttcggtccgg gaaattcggg attgacccga
    81001 aagaccggac agttctccac gcaaatcgta cagcgtatgc aatgttcaaa tttcatcgag
    81061 caggtactcc gcgcaggatt gtgctgcaac atgggctgtg gccagcgcga gaccgtggcc
    81121 gcagccgttt ttcagaattt gggtatcggc gagaatgctg ccgcagacaa aaatattttt
    81181 ccacggagca tcaagcgggc gcatggatgc gtcggccctg atgccggctt tgccgatgtt
    81241 gtgattcagg gaaaaataat cgttatcaaa ccaggtatcc cgggggccgg gaacataaac
    81301 cggcagattg aaaatatttt ccaggatgcc ctcgcgtgtg gcggtcagac cgccgctgac
    81361 aaaggagcct gtacacaaaa taaatgcccg ggcattgata ctgttgggtc ttccttcgga
    81421 gggcgtcgtt accgcttcga caatattccc cgtcttttcc atgcctgcca caggccatga
    81481 ccagtagatg gttccaccct tattgcgaaa cgttttcttc agcgcattga aaagtcttct
    81541 gccgggcatg gacggcggga tggtcggtat ttcaaaaacc ggttttcccg tccgttccga
    81601 tatcgtgtcg acgatgtctg ccgcggattc catgcccagc acggcaggaa aggcgatccc
    81661 gtcctcgtga atgccgcgtt gcgtcatcca ggcgatgaat ctttcgaaaa aatccttctc
    81721 ttcaaaacgg gcggcgatac tcatggtggt ggacacgcct gcatcataca ccgaaaaggt
    81781 cgtgctgcgg cgccgggaga cgatgtatcc gggatagaaa tccttcaatc ccttgaacgt
    81841 tataatatgg acgctcccgt catggttttc ctgaggcgcc gccgtcatgg tggaggggat
    81901 caggcaggaa atcttggatg tcccgagcgc ggaaagaatc agacgattgg cgtccgcaga
    81961 accgctgtaa ggcaatccgg cgtctttcat atctgcaaga aatttttcaa gagattgttt
    82021 gacagttttt tcctcaacaa gattgtaggg atgttccgga ggcaagctcc ttatgccgga
    82081 gagtggattt tttgacaggg aacagacatc gacgcagcca gtggaaagac acgcagtcgg
    82141 ctctcctttg gtcaccagcg cgactttcaa accgcgttcg gcaagaagcg ttcccgcggt
    82201 cagtccggct acgccggctc caatgatgac agcgtcgtat tcagtcacac tcattgcggt
    82261 ttctccatgg acaggatgcc cagatagatg tattcaacaa gctgttcttc acgaagctgg
    82321 tctccccaga gcgcgtaccg gatgcccttg aaacgccgct gcagaaatcc gcgcagcatc
    82381 tccatggatt gttcgggcga tatgatgctc atatcgttca tgatgccgag cgccctgaag
    82441 gtacagaagc cgccctgaca ggggcccatg cccagacgcg tccggtgaga aatatctccg
    82501 acgtttcggg ttgccgtctg ttggataatt ttctccacgt tttgtctggt aacaagttcg
    82561 cactcgcaga caatgttggt catgtccgcg agccgtttgg aaagcgggta gccactcaac
    82621 tcttcctgac cgtcgagcgg aatttccgcc gtttcacaag cttttttcag atgaaaaatt
    82681 gtcatgatgg tgtcgaccgt tttttccgcc atcagccgga aggttgaaag ttttccgcct
    82741 acgatgctca atagtccgtt ttgatgatcg ataatctgaa agccgcgggt gatttcatgg
    82801 ctgtcggctt tttttgattt cagcagaggc cgtacgcctg aaaaggccct gatgatgcgc
    82861 gttttgttga aatgaggaat ggtccgtccg gcttcgctga tgattgtatc cacttcggaa
    82921 gcttcgatca ggagattatc gggattttca atggccagcg atgttgtgcc ggccaggcag
    82981 acggcttcat tgggcacgat aatgtcgccg tccgaaggtt gtcttagccg gttgatgacc
    83041 atgttggtca ggcggtgatt cgtgatgacg agggatccct tcaccagcgt caaaggcact
    83101 tcggatccgg ccagcgttgc aacgatgtct gcccaggcgc cggtggcgtt gacgatgatt
    83161 ttggcgtgaa tttctttttc ttcgccggtg gtctgatcaa ccgccttgac gcccgtgcag
    83221 cggccgtggg atttgataat ttccgttact ttgtgccgtg tgaaaatcat gccgccgcgc
    83281 agttcggacg tccggatatt gagcaggcag agccggaacg gatcaatgga acaatcggga
    83341 acccggatgg cttcctcgat gtccggattc aggtttggaa cgagatcgat cgccagtgtt
    83401 gacgaaataa cttcggccgg aattccgacg tcttcgcatc cccgcagaaa taattcgcga
    83461 aaacgtttag aatctcctgg aaggcgcaca aacagtcctc ccgtttgttc aacgcacttt
    83521 ctgccgatcc ttctgagaat aagattttct tcgtaacatt ctcgggcggc taccggatcg
    83581 gtgacggcat acctgcctcc gctgtgcaaa agaccgtgac acgcgccggt cgcgcccgcc
    83641 gcgaagtcgc ctttttcaac gaggcagtga ggaatgccgc gcaacgcaag atctctcgca
    83701 attccgcacc ctgtcgcgcc tccgccgatg ataatcacat ccgtcttaat cattattatt
    83761 tatttttctc ttgtccttaa cgttgaacat tgaactttaa acgttgaacg tttcttattc
    83821 ctcttcttcc catttcatcg cccttttaac cgctttcttc cagttgttat aaagcgtatg
    83881 gcgtttatcg tcgtccatcg ccggattaaa acgacgatca acgttacgtc tccgggtgat
    83941 tgtggactga tctttccaga agccggtggc cagccccgcc agataagcag cccccatggc
    84001 cgtggtttcg atgatttccg gccgctcgac ggggacgccc agaatatccg actggaactg
    84061 cattaaaaaa ttattggcgc aggctccgcc gtccacacgc agctcgcaca gatccagccc
    84121 gcattcattg cgcatcagtt ccagaacatc cctggtttga taggcaatgg attccagcgt
    84181 cgcgcgaatg atatgatttc tcgttgttcc ccgcgtcagt ccgagaatgg cgccgcgcgc
    84241 gtacatgtcc cagtagggag cacctaatcc gacaaaggcc ggaacgacat aaacgcccag
    84301 cgagtcttca actctcgtag caaaatattc gctgtctttg gcgtcataaa taagtttcat
    84361 gccgtcgcgc agccactgga tggccgcgcc ggcgacgaag atactgcctt ccagcgcgta
    84421 ttccactttg ttgttgatgc cccaggcgat ggttgtcaga aggccgtgct gtgaatgaac
    84481 cagtttctct cctgtcagca tgagcatgaa gcagcccgtg ccgtatgtat ttttaaccat
    84541 acccggcgtg tagcaggcct gtccgaagag cgccgcctgc tgatcgcccg cgtctccggc
    84601 gatggcgatt tccgcgccga agagacggga atccgtatga ccgtaaacca tgctggacgg
    84661 tttgacgtcc ggcagcatgg aggcgggaat atcgagttcc tttaatatct tttgatccca
    84721 tttcaatttt ttgatgttga aaagcattgt ccgggaagcg ttgctgtaat cggtcacatg
    84781 cacctttcct ctggttaaat tccagatcaa ccaggtatcg acggtgccaa aaagcagctc
    84841 tccgtcttgc gccttttgtc gggcgccttc cacatggtcg agaatccatt ttactttggt
    84901 tccggaaaaa taagcgtcaa gcaccagtcc ggtattttcc cggatatatt tttccaggcc
    84961 ccttgccttg agttcatcgc agatatccgc ggtgcggcgg cactgccaga cgatggcgtt
    85021 gtataccggt ttcccggttc gtttctccca gacgacggtc gtctctcgtt gattggttat
    85081 gccgatggcg gcgatttctt ccgcggcaat agcggtggtg gcaagaagtt cgctgaccac
    85141 accgctttgc gatccccata tttccatcgg gtcatgttcc acccatccgg ctttgggata
    85201 aatttgcgta aattcctttt gtgctgttcc gacaatattt cccttgtggt tataaaggat
    85261 ggctcttgaa ctggtagtgc cctgatcaag ggataaaata taggattttt tcatgtctgc
    85321 gcccccggta tgatgataaa attggctgca tttgtaaaag cagtattgca taatgctttg
    85381 tttttgcaaa taaaaaaagt tcatgttgaa tgatgaactg cgatgtgtta aagtaatatt
    85441 acattgaaat aaagaataat ttattcaatc cgtatgaatc gctataagaa ttgccctttg
    85501 aaataaaaaa cagtctgcca agtgattgaa aacaataaga aaaaattaaa ctatcaataa
    85561 tgtgaaaata aattaaattt tttcttgaca caaccacaaa gagagagtaa atagcctttt
    85621 ctgttcatca gagtaagtgc ttggacataa gttgaaaact tcaatgagtt aaccgatagt
    85681 ttaattcgca gttatcggac acttccaatc gtaactggtt gaacaaggtt ttttgaaagc
    85741 ttgacaaaag aatctagctg cttagattta aagcataggc tcttttttga tagggctctt
    85801 tggaaattga atagtgggaa aatgaatttg tgggtccaaa caaatttatt tgagtaacgc
    85861 gaaaaataga aagagaaatc tttcttctag gattaaaact ggagagtttg atcctggctc
    85921 agaacaaacg ctggcggcgt gcc
//
LOCUS       DAOSME010000002        81268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_362_length_81268_cov_21.232438, whole genome shotgun sequence.
ACCESSION   DAOSME010000002 DAOSME010000000
VERSION     DAOSME010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 81268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 81268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81268
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_81268_cov_21.232438"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..499
                     /locus_tag="PLG98_00360"
     CDS             83..499
                     /locus_tag="PLG98_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31728.1"
                     /translation="MGFAIGQLISQIIGGAMAALLIALIIQWATKKVAKFKPSYGMAY
                     KSAFLGTVGGLILGFMLGFAFGMAGAGEAQQGGLILLSMIAGFLLQGAIYGQLLKDAS
                     SISVGLGKGYAIAGIQFAIGIGILVIITGAVLVIAS"
     gene            526..2508
                     /locus_tag="PLG98_00365"
     CDS             526..2508
                     /locus_tag="PLG98_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOE31729.1"
                     /translation="MKKVVLFLIAIFFPSYCFSANIKIDSEIISVPAYAGFVEISKIS
                     PDTMAMFKDMLPPTNNLHAVFVTASDSGKLLKSQSPELKRYLIVQTAKSLESVAFGAR
                     HFAELRTKIREEYESLFDQNKSKIDELAKNASQKLSNRISDKFEMKVGEMLPMSINSE
                     TAYHISLSSLTKFSTSTADENTDFISACTMSMVLLKGRVVYLNVFSTYNNLEDLKWTQ
                     SVAEKWTESTVRSNQNTQAGFGEIVPAGTRIDPEVKKVLSAEKKAYSTLGHPKSQGLN
                     ISVKYPSVWVAKEGQRPHIVQKFTGSSTGSIVPGCMIIIQELPSIGKIFLDGQMAEEI
                     FAEGVKDYIPPGAVYIDGGTTKIDAEPGAWVKYYFEQETSGVKIGMYSLQYILFYKGS
                     MFALQCSIAGSASEKLILEDAFSSYLPVFQNIGSSIYIPDKWNKEMSNTDVASSVMKD
                     TYGENWLLTLLFSAILTWGIGLAPPFLIRFAIIKHPLSKPPALITVIFLWVFNFVFFT
                     ALGSESKTHAGLLLVAWASYAILRKGSAKYEAEQTRHREEENLKAEEHRRKEEERRRA
                     EEESRRKKDRENEQKEKYNRERKSIKDEEYYQNILGLGSNFTVEDIKRRYRELVAKYH
                     PDKVNHLGEKLKETAEREMKEINEAYEYFKKKYNFK"
     gene            2614..3354
                     /locus_tag="PLG98_00370"
     CDS             2614..3354
                     /locus_tag="PLG98_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HOE31730.1"
                     /translation="MKPMNIDDILKDLTERAGSAVAHYWKTRAGQRDKQRLGGTADQG
                     LRSAVTGGAQMNGFIDLFTEFIVAVGIPEQCVFRKRAVELPGFFRPTKEWDLLVVREN
                     KLLAVIEAKSQVGPSFGNNFNNRTEEAMGSALDLWTAYRERAYLDSPQPFLGYFFMLE
                     DCDASNRAVKVQEPHFKVFPEFVGASYKLRYELFCRKLVLERHYTATAFIASTQDDGI
                     LGHFSTPADDLSLVRFARVLISHLAAFV"
     gene            3359..4267
                     /locus_tag="PLG98_00375"
     CDS             3359..4267
                     /locus_tag="PLG98_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOE31731.1"
                     /translation="MEETTHRLINGDARELSFLHDESVHLVVTSPPYWSLKRYNEHPD
                     QLGHIQDYEAFLFELEKVWRHVYRILVPGGRLVCVVGDVCVARRNFGRHLVFPLHADI
                     CVICRRIGFDNLNPIIWHKIANASYEVSNGSKFLGKPYEPNAIIKNDMEFILMQRKPG
                     GYRQPTNMQRDASRIDKEEFNRWFQQIWNITGASTKQHPAPFPLELATRLVRMFSFTG
                     DTVFDPFCGSGTTMIAALRTGRNSIGIDIDPEYCRMAARYLKAENADLFATTKLVFEK
                     ATAEPASLVKEDLALYEVRPARKKLA"
     gene            4338..4682
                     /locus_tag="PLG98_00380"
     CDS             4338..4682
                     /locus_tag="PLG98_00380"
                     /inference="COORDINATES: protein motif:HMM:NF022671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HOE31732.1"
                     /translation="MAFSEIELKRIEKEVGDFCEKRSPVEIRDKLSFQYRIKAHDVII
                     YTRRPRWDNPKEWMESDMAKIKYVRTSNMWQLFWQRANGKWFSYEPFPHSKNLKSIIE
                     EIEADQYGCFFG"
     gene            4770..5312
                     /locus_tag="PLG98_00385"
     CDS             4770..5312
                     /locus_tag="PLG98_00385"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOE31733.1"
                     /translation="MKKMQMDKAFTLMESGPVVLITTNDGKKNNIMTISWTMVVDFTP
                     VFAITTGSWNYSFAALQKTKECVIAIPTVDMLDKVVGVGTCSGSDTDKFEKFMLTPVK
                     GKHVKAPLIKECLANIECKVADIVRKHNIIVLDAMAAYIDDKRKEKRALHAVGDGTFI
                     VDGRKMDRRKMMASKLPAGV"
     gene            5380..6009
                     /locus_tag="PLG98_00390"
     CDS             5380..6009
                     /locus_tag="PLG98_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOE31734.1"
                     /translation="MKKFRIYCSGPLFCAEEIGGMSAIAKVLDDAGFQTFLPHRDGLE
                     SYILRFSNASFPSTISAIRTRVDYAIFSLDAYELIERCSAVVCNLNGRVPDEGMIVEA
                     SLAYAAGKPLVFFKNDLRAPFGGYDNSMLTSLVRGKIVKRLKELPSALEYELSREPEL
                     ISLPGGLKKAISYGKRISSVLENLQDKIFGKNSPDEIVSQVMEEALKEH"
     gene            6138..7445
                     /locus_tag="PLG98_00395"
     CDS             6138..7445
                     /locus_tag="PLG98_00395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOE31735.1"
                     /translation="MSQQIFSLSSWPRAIVHIDGDAFFTSCEEAIHPELRGKALITGG
                     ERGIVACASYPAKKLGIKRGVPLAEARKICPGLIVLPSDYETYSLFSQRMFNIMRRFT
                     PDVEEYSIDEAFADLTGMRRLLRSSYEEIVLKMKKEIERELGITVSVGLSITKVLAKI
                     ASKHQKPSGVTIIKGRDIASCLGNLPVEKIWGIGPATTNYLAKMGIRYALEFAQLPEK
                     KVREKFTKPGVEIWQELRGESVYPVTAEEKSSYRSISKTKTFAPPTSNADYLFAHLMR
                     NMESACIKARRYCLAPKKIVVFLKKNDFNTVGSEVKLSRPCAFPMEFSGVIRDLFDQC
                     YSVQDIYRATGVVLCELEPDEQVQYSLFDNPLQAEKIRSVYNVADELGRKFGKHALHL
                     GSSHPIDTLGRGRRGNPTVREQTRLKGETRRRHLSLPLLHVKT"
     gene            complement(7477..8037)
                     /locus_tag="PLG98_00400"
     CDS             complement(7477..8037)
                     /locus_tag="PLG98_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31736.1"
                     /translation="MNVKGTVFLTGKVAITANFGEARWNEFMAKLAEKDPFFKNVIMS
                     VTLVPVDKHLFFLDEMLKEFFRNDKSQFLLFGRVAAKFALSPGGPYHSYLLTKDIKQF
                     VESGMPKLWSTYYDGGKFTAKLENNIVHLKISDIPIKHIYFEYLIMGYFKQALKIFGK
                     ENIEKRVRSIASGDDDIYYQYEIKNL"
     gene            8394..9311
                     /locus_tag="PLG98_00405"
     CDS             8394..9311
                     /locus_tag="PLG98_00405"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOE31737.1"
                     /translation="MTLKDIKKVLILGAGTMGQQIGFLCAMHGYNVTMYDISEDILKA
                     SVARMEKLGSLWFVRINRITPEQLKEIMSRITATSDAKTAARNADLISESVPEDPKLK
                     GKIFAQFNTLCPKRTIFTTNTSLLMPSKFAEATGRPGKFAALHFHDVRTTNVVDVMPH
                     PGTDKAVTTLVHDFAVSIGQIPIMLHRESNGYVFNAMLSNLFMTALNLVTKDVASIED
                     VDRAWMGITMMPLGPFGIMDQVGLKTVWTITDYWADKMKDPQSKANANFLKSYVERGE
                     LGAKTKKGFYSYPRPAYSHPEFLTGQIKS"
     gene            9480..9806
                     /locus_tag="PLG98_00410"
     CDS             9480..9806
                     /locus_tag="PLG98_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31738.1"
                     /translation="MAEDDDVLTTDDNESPVSGYLSIVLTIIGLVMLLLIKILGITSS
                     DETGWSIEFVLYLFISMTFAIAGGFLGFLGLSEDNNKKIFPIIGLIVAAIYVVSLGYV
                     IATSIS"
     gene            complement(10019..10990)
                     /locus_tag="PLG98_00415"
     CDS             complement(10019..10990)
                     /locus_tag="PLG98_00415"
                     /inference="COORDINATES: protein motif:HMM:NF021550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HOE31739.1"
                     /translation="MKKCKDIENNLSLYLDDVLSKEDKAAVDEHLRSCRQCEEALARL
                     RKTKELTNSLHEVEPPPWFRKKIMARVREEAGKKNFLYKLFYPLQIKIPVQIFATVCI
                     AVLAVYLYRAGENRFQEVVPSSVPAPVLQTQQDQSPEAKENTSLAERTQPEPNTISQK
                     DKPRESVRKKEVHRVTDVHETDVNELKASDGRDDSNKIAPAQVPEQFSVESAQTKDRH
                     VSGTALKASVAPRAQDSHIKPGIVLKVTDLEVAVREAEQIFRQCEAKNITATIMRDKA
                     FLTGELNRHNIKDLLRQLSTIGRVEEKHIPAADAEDNTVLVVEITNR"
     gene            complement(10987..11607)
                     /locus_tag="PLG98_00420"
     CDS             complement(10987..11607)
                     /locus_tag="PLG98_00420"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE31740.1"
                     /translation="MSNIINTRAKTAKDEDLKYVTLCQKGDTESFDVLVERHQKKMLN
                     IAYRMIGDYDEACDVTQEAFVAAFKSIRQFKAEAKFSTWLYRIVVNYSKNRLKRLSGR
                     SRREGFSIDATGGRNADQLSDQGMSQESNPGVQMEKREREAYVQKCINTLDAEHRDVL
                     ILRDIQGFSYEEISAILKVPDGTVKSRLSRARNALKDCLQKVIGDL"
     gene            complement(11713..12474)
                     /locus_tag="PLG98_00425"
     CDS             complement(11713..12474)
                     /locus_tag="PLG98_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31741.1"
                     /translation="MAIGILAALIFYHFTKPPRQATPKPVPAAPAAADSPAALPPVQV
                     FTPSDAPPPPEAIPPLPTRQASPKPDKMKMVEQIAAEFHKGHTYTLEGEFVCLDMAID
                     VWNQLVTNGIEAKIMGGTLHESITAWNFRQLAMGSNHAWVVATVGPMEKVAIETTSGT
                     VIKPGMKEYASYFKGIEFDSPAQIKRFELLRRKMNDVCRDAHQMIQDWNQNVAGKQLH
                     PAEIVARQSRLEQRKQDCENTFRELREFESKAIFY"
     gene            complement(12675..13145)
                     /locus_tag="PLG98_00430"
     CDS             complement(12675..13145)
                     /locus_tag="PLG98_00430"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE31742.1"
                     /translation="MTMDAVPIDVILEQLKIVYEEKMPFNKYLGLKIDSIKADKAVVR
                     IDMRDDLIGNFEKNILHGGVISTILDFTGSVIAQIHIINEMKDAPLEKIVKRLTGMGT
                     IDMRIDYMRPGKGSYFLATGHLLRLGHKVAVARTEFVNDSGELIAVGTSTYIVG"
     gene            complement(13183..14472)
                     /locus_tag="PLG98_00435"
     CDS             complement(13183..14472)
                     /locus_tag="PLG98_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOE31743.1"
                     /translation="MSINVKKNSKTWVVYILRCCDGSLYTGITNNIEKRLAAHHQGKA
                     AKYTRSRRPVELLVTGTAMERSAAMRLEIKIKRQPKDKKVSALKKHAPRMSSTAATTS
                     KRRVVAAGEHSLFGKETLKAVAGLWQVPLKKIHRKIPVQGSPERSAFRVVLEDTDKRF
                     FVLEQIASQSAAGRKQIAAILDLLAAKKLPQINPYLTDERGNYLLEHQNNFWQLSPFV
                     PGVELDREKYVFEPWRGIVLADFLIALRRKSQKMTLAGSRGVFSLRNYIYKLVREINL
                     YNKDIKNDIGSVVDFLEKDFLPVYEELPVAFCHGDYHPLNIIWSTDGIRCVIDWEFCG
                     LKSELYDVANLIGCIGMENPRSLAGGLVGHFIDRMKRSGIMSQISWRYLTEFIIALRF
                     AWLAEWLRRKDRNMIRLERDYMKLLMDNKTVLQKAWL"
     gene            14823..16100
                     /locus_tag="PLG98_00440"
     CDS             14823..16100
                     /locus_tag="PLG98_00440"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE31744.1"
                     /translation="MSFEKFELDAKILAGVQACGYNVPTPIQLQSIPPIMEGRDVMGL
                     AQTGTGKTAAFVLPILQRLMQGARGHVRALIIAPTRELSEQTHTAIMQLGRKTKLRSV
                     SIYGGVNINGQINKLRNNVEIISACPGRLLDHINRGTVDLSRIEVLVLDEADHMFDMG
                     FLPDIRKIVKHLPAQRQTLLFSATMPDDIRSLANDLLINPVNIKIGHTAPVETVSHKF
                     YPVQMSSKTDLLKDILDKTDMDSVLIFTRTKSRAKSVALQLGKSGYKVTSLHGNLTQQ
                     KRQHALDGFRDGRYEIMVATDIAARGIDVNSISHVINYDMPSTTDAYTHRIGRTGRAA
                     KTGDAFTFVTGEDRGLAQSLERVLGKKLHYQNVDGLTMSLPVDDRVSASRNSRGRNMK
                     PHNDRKKFSPKARANSRLNRRSFDSSNRSQWAH"
     gene            complement(16193..16648)
                     /locus_tag="PLG98_00445"
     CDS             complement(16193..16648)
                     /locus_tag="PLG98_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOE31745.1"
                     /translation="MRKKERAIKDRKDIDGIIRRCRVCRLAMSDEDQPYIIPLNFGYD
                     GFCLYFHAAPEGRKIDILKRNNRVGFEFDILHEILDAAQPCAWSAKYESVIGSGTAEI
                     INDVKGKKAALECIMRQYGSDAGDFSEKVMKKTLIIRVRIREISGKARR"
     gene            complement(16693..17532)
                     /locus_tag="PLG98_00450"
     CDS             complement(16693..17532)
                     /locus_tag="PLG98_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE31746.1"
                     /translation="MTKHLFYPVKASIKKSYIHGYDPVENIRLQDQASTLVELLHVDT
                     AYAPGSTVLEAGCGVGAQTITLAKNSPGALITSIDISAASIDEARRKTEAAGIQNVHF
                     EQADIFNLSFASQSFDHIFLCFVLEHLPQPVEALIILKRYLKKNGTVTVIEGDHGSAY
                     FYPDSAAAHKAIESQVELQRRAGGNANIGRALHPLLNQAGFRSVCVSPRMVYVDSGKP
                     QLVDGFIKKTFTAMIEGVRAEAIDAGIIGKNVFDQGIRDLYRTAEPDGLFCYTFFKAT
                     GYK"
     gene            complement(17554..18420)
                     /locus_tag="PLG98_00455"
     CDS             complement(17554..18420)
                     /locus_tag="PLG98_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOE31747.1"
                     /translation="MRWRGERQSDNVEDRRGMSISRGAKVGGIGGLGLVAIVVISMFM
                     GVDPSSILQVVEQNTQSSSISVEQSATTAANDNARNFVAVVLAETEDVWNDVFQQAGR
                     TYEEPKLVLFSEAVESACGMAGSATGPFYCPADHKVYLDLAFFEELHSRFGASGDFAQ
                     AYVIAHEIGHHVQTQLGIIQKVHELQSRTSKSEANKLNVKLELQADCLAGMWAHQADK
                     KRNILEAGDIEEGLNAASAVGDDRIQKQTQGYVVPDGFTHGSSAQRVTWFKRGFEQGN
                     IGACDTFNAARL"
     gene            complement(18689..19207)
                     /locus_tag="PLG98_00460"
     CDS             complement(18689..19207)
                     /locus_tag="PLG98_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOE31748.1"
                     /translation="MVKLYRCIICGDAYIGASPPANCPFCGAHMEYIMEAKDAVVNFD
                     VPLSAKDRANAEHALKVEISNATFYSCAAGKTDDPEGKVLFKALGKIEAEHASIWKKI
                     LKLASLPPGNETCHTENVGNLKESHARETRAIEFYRKSAAEADHPRIRQLFEALVEIE
                     TDHLFLSEERLH"
     gene            complement(19243..19620)
                     /locus_tag="PLG98_00465"
     CDS             complement(19243..19620)
                     /locus_tag="PLG98_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOE31749.1"
                     /translation="MMANRNEVYKCNVCGNIVEILHAGVGKLVCCDQPMKLMTENTTD
                     AAKEKHVPVVEKVAGGFKVTVGSVAHPMEEKHYIEWIEVIADGKIYRQYLAPGQAPEA
                     VFPIEAISITAREYCNLHGLWKA"
     gene            complement(19643..20140)
                     /locus_tag="PLG98_00470"
     CDS             complement(19643..20140)
                     /locus_tag="PLG98_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOE31750.1"
                     /translation="MSKTEEALKEAFAGESQANRKYLAFAAKADQEGFAQAAKLFRAA
                     AEAETVHAHAHLRAMKGIRSTKENLQEAIAGETHEFKSMYPAMIEAAKAEGHKEAERS
                     FNFANEVEKIHAALYQKALDHLDGSQETYSYYVCPVCGYTVENEAPETCPVCGAKGKM
                     FKKVD"
     gene            complement(20261..22468)
                     /gene="katG"
                     /locus_tag="PLG98_00475"
     CDS             complement(20261..22468)
                     /gene="katG"
                     /locus_tag="PLG98_00475"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HOE31751.1"
                     /translation="MTEESKCPVTGMMKKPISGGGKSNRDWWPNQLNLKILHQQNKKS
                     NPMGEAFDYAKEFKKLDLKAVKKDLITLMTDSQDWWPADWGHYGGLFIRMAWHSAGTY
                     RMGDGRGGAGTGNQRLAPLNSWPDNVNLDKARRLLWPIKQKYGKKISWADLMILAGNC
                     ALESMGFKTFGFAGGRVDVWEPEEDIYWGSEEEWLATSDKPKSRYSGERDLENPLAAV
                     QMGLIYVNPEGPDGNPDPIASGRDVRETFARMAMNDEETVALVAGGHTFGKCHGAGPA
                     THVGPEPEAAPLEAQGLGWGSSYKSGKGGDTISSGLEGAWKPNPTTWDMGYLKVLFKY
                     EWELVKSPAGANQWLAKNVEDEDMVADAHDPSKKRRPMMTTADLSLRFDPIYGPIARR
                     YLANPKEFADAFARAWFKLTHRDMGPRSRYLGPEVPKEVLIWQDPIPAVNHKLINKAD
                     ANSLKSKILASGLSISELVSTAWASASTFRGSDMRGGANGARIRLAPQKNWEVNEPER
                     LAGVLKTLERIQKAFNKDQKGDKKVSLADLIVLAGCAGVEQAAKNAGYKITVPFTPGR
                     MDASQKQTDVASFGVLEPKADGFRNYQKAKYTVLPEEMLVDKAQLLTLTAPEMTVLIG
                     GLRLLGANFGGSPHGVFTSRPGTLTNDFFVNLLDMGTEWKVSKDADVFEGRDRKTGEL
                     KWTGTRVDLIFGSNSQLRAVAEVYACKDSQEKFLDDFVAAWNKVMNLDRFDLA"
     gene            complement(22633..23061)
                     /locus_tag="PLG98_00480"
     CDS             complement(22633..23061)
                     /locus_tag="PLG98_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOE31752.1"
                     /translation="MIYKDGLIGQLKEKGLKITPQRLAIIDALVEHSEAHPGATLIYE
                     AASKKARRISLSTVYATLKEFSENGLIRQLEFDRMENRYDVDLSDHVNLICNRCGKIF
                     DYAIPASLEPRDIARKSGFVVTETRLEFHGYCRDCLKRSD"
     gene            23208..23537
                     /locus_tag="PLG98_00485"
     CDS             23208..23537
                     /locus_tag="PLG98_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31753.1"
                     /translation="MPVYLQVILLVAIFIATVIIFLYIGGLAVRRASFQIIAEMEEAR
                     AFKEGRAIKIQDERKNFFRVGTKNIRPRALNLLIADGLVIKTNNGKYYLDKEKLAQAK
                     AQLSSKT"
     gene            complement(23559..24704)
                     /locus_tag="PLG98_00490"
     CDS             complement(23559..24704)
                     /locus_tag="PLG98_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31754.1"
                     /translation="MNRKTELEEITHTGGKVNFNIKIDADGRIAYNVGLTHSRPTPAA
                     LFAVYAIPQGVAVHDIKMGGIGTPWNPPPLPDCFPVFISSDSTGMFGHQCPSCNGYWR
                     AGLGGKICPYCAFTADEYYHFLTDAQQRYVRQYCEVLSNALASGQSGEHIIDMDSVAE
                     AAGKDCEKPAFYYAEERQQNLFTCNACGKVNDVLGTYAYCSSCGTRNDLQELEKNIQQ
                     IRDRINAGGPYETCVKETVAIFDSFAGHYAKQLLTRVPLTPARKAWIEKSHFHNIGTV
                     SEMFRKVFDIDVLSSIDSEDVSFGTLMFHRRHVYEHKGGEADEKYITDSGDSVRLKQA
                     LRETKETAHRTANFITKIATNLHKGFHEIFPPLEEPIKQYEQRKYKR"
     gene            complement(24976..26856)
                     /gene="mnmG"
                     /locus_tag="PLG98_00495"
     CDS             complement(24976..26856)
                     /gene="mnmG"
                     /locus_tag="PLG98_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOE31755.1"
                     /translation="MTNLTDNYDVAIVGGGHAGCEAALACARMGCRTILFNINLDSIA
                     LMSCNPAIGGLAKGQLVKEIDALGGEMGKITDKTAVHFRLLNASKGPAVHSSRMQCDK
                     QLYRLTMKAVVENQPNLYVRQAMIEKLVIEDGKVIGVLDQSEHFYGAKKIIITTGTFL
                     NGLVHVGPSQTPSGRAGELASIGLANHLKELGFDIGRMKTGTPPRLRASSIDFSRLER
                     QDSDPDPRPFSFTTEKLRTERLPSYFSATSAETHRVIRENIQHSPLYSGIIKGVSARY
                     CPSLEDKVMRFAERQSHPVVLEHEGLDTQEVYAKGLGNCLPLELQYKIVRSVKGLEQA
                     QIMRPAYAIEYDFVQPTQLKPTLETKLVDGLYLAGQINGTSGYEEAAAQGLWAGINAA
                     CAVQGAPAFLLDRSEAYMAVMIDDLVTRGVDEPYRMFTSRAEYRLILREDNATIRLMG
                     KGCELGLIDKTHYLELQDKIKKIENGIEQLKASPVKPTAETNEKLATLNSPPLSNHTT
                     LHGLLKRHELSYDNLSIFPGWEPELDAFVKQQIEIETKYEGYIKRQFDAVKKLKDQEK
                     KKIPPDFDYNSVPGLSNELKLKLTKIAPQTLGQMERIPGMTEGAISAVLIMMKKHELQ
                     GR"
     gene            27057..28010
                     /locus_tag="PLG98_00500"
     CDS             27057..28010
                     /locus_tag="PLG98_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418923.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOE31756.1"
                     /translation="MPKPDFWKNNKNYYDLKSYWRNLFGCSVHKLQIDAGFTCPNRDG
                     HVAMGGCIYCDGRGSKLRQAGELPSVTEQIASGKKYYTKQAGKFIAYFQTFTNTYAPV
                     EKLRALYDEALNQEDVIGLSIGTRPDCLGSDVVELLSGYAQKYHVWVELGLQSVHDKT
                     LTLINRGHSFQQFEDAVNALAGHGLNICVHIIIGLPGESDEDVLATAKTLAAMPIHGI
                     KIHSLLALDGTALGEMYKKGRINMITKEKYVSLVADVLEVLPPQTVIQRLTADGYRDI
                     FLAPDWAMNKLDVLNSIDKELIRRDSYQGKKYRTNRAESLD"
     gene            complement(28051..28257)
                     /locus_tag="PLG98_00505"
     CDS             complement(28051..28257)
                     /locus_tag="PLG98_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31757.1"
                     /translation="MEEKERMIDRLFNPMLDALEDHPYSGMLVIAFVCILIVAFFPPS
                     LVFILIFIGYGFYLNSKRSKKDKP"
     gene            complement(28615..29388)
                     /locus_tag="PLG98_00510"
     CDS             complement(28615..29388)
                     /locus_tag="PLG98_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31758.1"
                     /translation="MMKKMFLSSLVVFIAASIVSSPPVCFSEQPDPKVWKHLSDDFYY
                     NKINLIQSADSISVWTYRTIADDERKYLIEHFRESDPKKSIKYQKLDHQTILLKMDCR
                     NKQSKIEKLVNYDDNESVLYEETYNNSEWTGIIPGSKPEEIYHDICVTSERQEQLHAL
                     KMKCEKDCDAWVRVDEKKFFRDPFTHQSHYNTRLDKCFILLNYTKKPLKVLREMKGNK
                     IYGAVRSQKNGKTITCNVLEKTCKSEAEWDAHVKPYMEE"
     gene            complement(29600..31417)
                     /locus_tag="PLG98_00515"
     CDS             complement(29600..31417)
                     /locus_tag="PLG98_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE31759.1"
                     /translation="MTENDIKTLPQGLKWLVDKQPDRVAMRMKDYGIWHDISWRQYYE
                     NVKKVAVGLYALGVKPFDHVAIVGENKPEWLFSAMGVMSLGGIFVGVYTTNPTAECEY
                     VVGHSESVVFFCEDEEQFDKALAFRERTPRLKKIIVWDMEGLKHFDDPMLMSFEKLLE
                     LGEKIDKERPGLYEKIVAGRESEDTASIIYTSGTTGPPKGAQISHQNYLWIARQSQKI
                     TKLSVDDETISFLPLNHVYEQIFDLMMHLTTGHIVNFTENTDTVMNDLRDVSPTLFHA
                     VPRIWEKYHSGIVLKMADATWLKRTVFNLAFKIGTTYNGFKLAGKPIPWYLFLGYQLA
                     YFSVFWKLKQRLGFDKVRLGFSGAAPISHEVLKFFQSIGIPIREGYGLTETTGITHMS
                     DEEHFKVGTVGRALPDSEVKIAEDGEILIKHGGIFQGYFKEAEATAEALEGGWFHTGD
                     VGEIDAEGYLKITDRKKDLIITAGGKNIAPQYIENLLKFSPYITDAVVIGDRRKYITA
                     LIVIDEENVVKYAQDHKIQYTTFASLTRAEEVIRLISDEVEKANRQIARVENIRKFHL
                     LDKKLYTEDGEVTPTMKVKRKSISAQYKDIIEAMYTEDP"
     gene            complement(31427..32197)
                     /locus_tag="PLG98_00520"
     CDS             complement(31427..32197)
                     /locus_tag="PLG98_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE31760.1"
                     /translation="MLSVKNIETWYDLIRALHGISFEIKEGSIVALLGSNGAGKTTTL
                     KTIMHLLDDDQPEKGSIEFLGKRIDRMDTEEIVRLGISYVPEGREVFPELTVMENILM
                     GAYTRKDRKGIQDDIDGILKQFPILSERKNQQAGHLSGGEQQMLAIGRALMSRPKLLM
                     LDEPSLGLSPLLTKEIFSIIQNINQKGVTIFLVEQNVNMALKYSHYAFLMENGRIVRA
                     DRPEILREDDDVKEFYLGIATETSVKGFKRYRRKVRFR"
     gene            complement(32197..32970)
                     /locus_tag="PLG98_00525"
     CDS             complement(32197..32970)
                     /locus_tag="PLG98_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE31761.1"
                     /translation="MAHFKVENLNISFGGIRAVSDVSFEVEYNKIFSIIGPNGSGKTT
                     IFNIISGIYKPHAGEVFLDNENLVGLRPDTIARKGVARTFQNIQLFGNATVMDNLMLG
                     RHIHMKTGITSGAFMWGKRSFAAKEELKHREIVEHIIDFLDLQSLRDLFVSSLPYGKR
                     KLVELGRALALEPKVLLLDEPSAGMNTEEKDDLRIWIKDIQEDYKVTILLIEHDMNMV
                     MGISDRILAINQGVKIIEGTPQDVQKNPEVIAAYLGEEE"
     gene            complement(33056..34246)
                     /locus_tag="PLG98_00530"
     CDS             complement(33056..34246)
                     /locus_tag="PLG98_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE31762.1"
                     /translation="MKRFIIALTIVIAVFIASLLVFAQAKSGFDNKEIRIAQWGPQTG
                     PAAPWGSVARGSGLLFQIVNDEGGIHGRKIKYFIRDDQYNPAQTKAVVKELVEREGIF
                     AFVGGVGAACGMAVKDYLAQNKIIWVNPSTAIDEYVFPVNPYLFAMYTLYDDEASILT
                     KYTVEKLKAKKIGFLYQNDAYGKSGLKGCKARLNTYKMKLVEEISVEPGEKDLSSQML
                     RLKNSGAEVTFLWVNPTSAAIALKTAATIGFKPQWISSDTLMDFPLMNKITGGLWEGV
                     ITGGIGLPTDVDHPLMNKYRAAAKKYAPEERFGVFYTAGIMFAEPLVDALKRVGPKLS
                     TEALLRELNKTKNFQGLGAVVNWSPTVHQGCDSIQILKCGPNSSYIILQDWTSNELAT
                     WKKK"
     gene            complement(34315..34764)
                     /locus_tag="PLG98_00535"
     CDS             complement(34315..34764)
                     /locus_tag="PLG98_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31763.1"
                     /translation="MSKTVKILILACAVLVVFGLVIWDAPRNRLELFLEKSVIEETAR
                     NYLTAEMKKDSRQVYALLAPSSDYKKTHTYEEYLKDIAENSSAALNTCKIVRIYHLRD
                     NHNRHNYPAVDKLVQVEVEVTFGHAGPNSIFNYSFTFLKEKGKWYKG"
     gene            complement(34787..35659)
                     /locus_tag="PLG98_00540"
     CDS             complement(34787..35659)
                     /locus_tag="PLG98_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE31764.1"
                     /translation="MFEMLLIEGIAIGACYGLFGVAVAIIYKTSDVINFAQGEMGMVA
                     TFVAYHVLTVYNVPFWLAFPVTLIFAILLGMAIEFCFLRPAKDPTVLGLLIITLGAEM
                     LLMGLAGWKWGAEQKDFPFLLSVTKTHEMLPGIFINEWTIGVFSLSIIIMISLFLFFR
                     YSKLGIAMRATQQNVNAAKIMGIKVERVFSITWAMSSLIGAVAAMFFAARSVLDPNFM
                     MEPFMRAFAAAVLGGLTSIPGVVVGGLILGIIENFFGYAWPEWKPIVAFVVIILIITV
                     RPSGLFAKHYIKKV"
     gene            complement(35681..36751)
                     /locus_tag="PLG98_00545"
     CDS             complement(35681..36751)
                     /locus_tag="PLG98_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE31765.1"
                     /translation="MDMKRDYYEDVELFRSGTILTWSIVLLVFLFTLPVYLPSYNIYL
                     LNIIMVNIILAVGLNILVGYTGQISLGHAGFFAIGAYGTSLIMMNFHFPFFAAIILAA
                     LIAAFFGFIIGLPALRLEGPYLAIATLGFGLTIMHIIGRWDFFGGRMGITAPPLDMGI
                     SGTSFSYVIQGDVKIYYLILIMTVLLVIGARNLMKTRVGRAFMSIRDDDIAAEVMGVN
                     LTIYKTLAFAVSAFYAGVAGGLYGFVVGFFDPFTFNLILSIIFLVMITVGGLGSIMGA
                     IMGAMLITYLQYDLLKNVENLPYLGALLTTVSKKWFTVIGLANFSSIALGLIMITIVI
                     FEPLGMFGIWIRIKKYWKTWPF"
     gene            36979..37605
                     /gene="hisH"
                     /locus_tag="PLG98_00550"
     CDS             36979..37605
                     /gene="hisH"
                     /locus_tag="PLG98_00550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOE31766.1"
                     /translation="MIAIIDYNAGNITSVARALQSIGQEFIITDDAKKLDAASRVIFP
                     GVGAAGEAMAYLRQKKLDVWLRDYIKTGKPLLGICLGTQIILDYSEENDTPCVSLVAG
                     STRRFPETLAFNGELLKIPHMGWNSVRLLRSHPVFDHVSPEAEFYFVHSYYPAPADEA
                     AILGVTEYGITFCSVLARNNLVAMQFHPEKSGRPGLQILKNFCTWRPS"
     gene            37602..38381
                     /gene="hisF"
                     /locus_tag="PLG98_00555"
     CDS             37602..38381
                     /gene="hisF"
                     /locus_tag="PLG98_00555"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOE31767.1"
                     /translation="MISKRIIPCLDVRDGKLTKGIKFKGNVDIGDPVEAAREYYAQGA
                     DEIVFYDITASSDKRNIMIDVVRRVAETIFIPFSVGGGIRNLDDMRNVIHAGAEKVSV
                     NSAAVENPQIITQGAKAFGSQCVVLGMDVKQVDVSDAIPSGYEIVVHGGRIYTGIDAL
                     WWATEAEKLGAGEICLNSIDADGVQTGYELNLTRLISENVHIPVIASGGAGKPEHLAD
                     VLTKGKADAALIASMVHYRTYTIAEIKHYLSEKQIKTRLFW"
     gene            38460..39341
                     /gene="pdxS"
                     /locus_tag="PLG98_00560"
     CDS             38460..39341
                     /gene="pdxS"
                     /locus_tag="PLG98_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOE31768.1"
                     /translation="MDKKRYELNVQLAQMLKGGVIMDVTNVEQAKIAEEAGAVAVMAL
                     ERVPADIRKDGGVARMSDPAMIAAIKRAVSIPVMAKARIGHFVEAQILEALRIDYIDE
                     SEVLTPADEECHIDKTKFSIPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRAMNREIGQLVQMPVEEVTGFCKTNGIPYEHALKVKELSRLPVVNFAAGGIATPA
                     DAALMMQLGCDGIFVGSGIFKSADPARRARAIVKATAYFNDPQKVLEASMDLGEAMPG
                     LEISQIPPEQILAGRGW"
     gene            39348..40037
                     /gene="pdxT"
                     /locus_tag="PLG98_00565"
     CDS             39348..40037
                     /gene="pdxT"
                     /locus_tag="PLG98_00565"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOE31769.1"
                     /translation="MSLRLVSDKPSVIGVLDLQGGVHEHLEHLERLGVACKRVKQTED
                     FKDLAGLIIPGGESSCLSRLLTIFGMKDVLLAAHRRGMKIWGTCAGAILLAQNVVGED
                     ACLKLIDITVERNSFGSQLDSFAGEALIPGISADPVPLTFIRAPKILAAQPDVRVLLK
                     IDDYIAAAENDSVLVTVFHPELTGCLALHQYFAVKCGLKTATGQAGHSDPDWDISSWT
                     KYAALAGRAGD"
     gene            40160..41266
                     /locus_tag="PLG98_00570"
     CDS             40160..41266
                     /locus_tag="PLG98_00570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE31770.1"
                     /translation="MQNIFSELKTDSRFIRFVMKVGSPSPLDPDEQHRRIPAVFVLMI
                     ISVATAVSGIYHCSSGKNPLVVLDVIGFILSLIMLLYLRQGKNPGIAYGMISTGFVLL
                     CCAATVLGRTEMSLIFWALVLPAGCFSILGRKMGMFVAAFFFFINLLLMNLPEHMILS
                     KPFPASVAARFSVVYLILTFMLYYYESSHQMLIRYIRHEENKFQNASRYDPLTGLSNR
                     RDILEKMENERERHSRLEKTFTLIMGDIDNFKMLNDTLGHEAGDYVLQTIGRILKNQV
                     RGIDCSSRWGDDEFLILLVDTDLEGGQRVAERIRKKVENTSFQYQGKGLAVTMTFGLS
                     IYQSADEQIDACVRRSDDALEEGKRQGKNRVVPV"
     gene            complement(41327..42691)
                     /gene="mgtE"
                     /locus_tag="PLG98_00575"
     CDS             complement(41327..42691)
                     /gene="mgtE"
                     /locus_tag="PLG98_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOE31771.1"
                     /translation="MQKVKKKEPTALETLKDLISRNREFDIIDLFSDMHPADIADLID
                     ELEEEDRLHLFSLLDVDKASDVILELSDTSREQLIEDLSNEKLTDIIEEMDSDDKADI
                     IAELSDDQAKAVLEAIEPEESQEVQELLKYADDTAGGIMQSELVSVRKDATINDAFQA
                     VADAKDEIENIHNIFVVDETDKLIGTVPLQSIITTKRFTPILDIIDEDIPSVLHDMDQ
                     EEVARLFKKYDLVSIGVVDNQQRLLGRITIDDVVDAIQEETSEDIYRIAGLGEDDTVF
                     NNTTESVKKRLPWLYLNLITALASVLVIGFFEETIKLMVALVFFMPVVAGLGGNAGSQ
                     TLTLIVRGMALGEITFENAKRALFRQIAVGIANGIAVGIVIGIIAYFWKGIPILGLIL
                     GLAMIISIFTGTLVGALIPLALKRLKLDPALGSHIFLTAFTDAFGFLTFLGLATIFLK
                     IFHL"
     gene            42986..43816
                     /locus_tag="PLG98_00580"
     CDS             42986..43816
                     /locus_tag="PLG98_00580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE31772.1"
                     /translation="MTCLYSLREFYFLGAKGTILIKADNIEKNFFSVGRYWGNLNASL
                     KRKESVWWMNTGVAVSDMNWAWNETPLAAPDTAIVSEIREDYKKNNLRFWWWVYPCGQ
                     SAATSAILQNAGFRLYANVCCMAADLNESFPEPYATGDMEIYHVRSREDLAVWEDVAF
                     HGFEMPRHARDPFGAFVSSFETGDQFPQRFILGCIQGKPVATSLLFKDQRTAGIYYVS
                     TLPSYRNRGCGLSVTQAAMQLARESGFKHVVLQATPMGKRVYERLNFKRYYHAGIYTL
                     "
     gene            43954..44844
                     /locus_tag="PLG98_00585"
     CDS             43954..44844
                     /locus_tag="PLG98_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31773.1"
                     /translation="MKRNSGHNIACDNECGGLHQSNPLSLAKNEISSTLSWFMTDNAL
                     YPGVDKHSSPLRYENWSGRYSEYISRQEAESIVSRFICPEKDENFTLFFVSDYKDTYL
                     GKNFLKDGSDIYLKHYRMTDAPREIRLRNVLRRCLARKNFNAFYQLHQKNIEAVKPLF
                     YVMKRGRWIPDEVIAVSKGASGNKTIETLMADSRDNDEFRLLAVNLGSYVAELQSREI
                     LFKELYRNLIAVNQTTHWDFILCDLDEIRSVSEKRFYKHRKHVEKLRKKIKRYGDSFL
                     HAFDQGYQTRMHDQTAPSRR"
     gene            complement(44904..45248)
                     /locus_tag="PLG98_00590"
     CDS             complement(44904..45248)
                     /locus_tag="PLG98_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOE31774.1"
                     /translation="MGKMTQCPWGSFEVLSETAGHKVKRIIVEPGGGLSLQLHHFRSE
                     HWFVVAGTGDVTVGDKIISLSAGGSVDIPAKVKHRVLNHADGHLVIIEVQTGARCDDG
                     DIERFEDLYGRI"
     gene            45401..46087
                     /locus_tag="PLG98_00595"
     CDS             45401..46087
                     /locus_tag="PLG98_00595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOE31775.1"
                     /translation="MNISYTVRRSRKRQKTMSLQVCDDAKIVVSAPSFTPADDIRRFV
                     EEKQGWVAKILARQKQEAAHAKEKKYETGEQFFYLGQLYPLEVFFEPFEKEGIAFRNH
                     RFYLNCRSDKVLRKQCFVAWYSKKADEMIRRRVDFFSGMLKLPYERIRITSAGSRWGS
                     CSEDNSLSFSYRLIMAPPDVVDYVIVHELVHIREKNHSSTFWQKVEAVMPSFREQRRW
                     LQDNHSNLAL"
     gene            46121..46978
                     /locus_tag="PLG98_00600"
     CDS             46121..46978
                     /locus_tag="PLG98_00600"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOE31776.1"
                     /translation="MEHIEIIDLKHRDGLAERFKKMNLRISDYTFANVYLFRNISHYE
                     FVTKECGLFVSGWNKQNQNYVMPLNDPRGCDVKTFAEILGARNFFFPIPEEWLVYFPK
                     DKFSVSYDESESDYLYLTENMAGFKGKQYLRHRNHLKQFLASYIPDGVTLSSHNCNDA
                     MIVLHQWQEDSRTASDKTDFVQCSEALQNFSGLALWGIIYYIAGKPAGFIIGEALAGD
                     TFCLHFAKASKRYHGIYEYMFNDTAKRLESAYKYLNLEEDMGNKNLRRTKSSYGPEFL
                     LKKYRVGII"
     gene            46979..49234
                     /gene="feoB"
                     /locus_tag="PLG98_00605"
     CDS             46979..49234
                     /gene="feoB"
                     /locus_tag="PLG98_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOE31777.1"
                     /translation="MEKKTAMSESVNITSLQEGDQGVIHAIEGGSAITSRFAGMGIVS
                     NARFRIAQISGGLIVLQVANTRIALGRGEASKITVSKVAPEADVCMPPIEKEISVALV
                     GQPNVGKSTVFNILTGLSQHVGNWPGKTVEKKEGTHRADSVLIRIVDLPGTYSLTAFS
                     EEERITRDFIIKEQPGLVVLVLNATALERGLYLLAEVLLLNRPVIVVLNMLDVAADQD
                     IEINIRALEDSLGVPVIPMVAKRNSGIRELVSHISSIASSEYKFQPVKPEVSTDHQLI
                     YQQILTLVRPYIHDPYTPEWVAVKLMEGDPEVTQLVEQQVQTIARDQIEKLLIKHEDA
                     LHAVVNGRYDWIEEITRSVVSRFKMGQVVLTDRIDHVLTRPVFGIPILLAVMALVFFL
                     TYSVGVPLQEWLSDRIQQFIGFAEPLTTVWPSWLSGLLLNGVIGGVGSVLTFLPVLLI
                     FFAIMAFLEDVGYMARAAFVMDKIMHLVGLHGKSFIPMCLGFGCNVPAVLGARIIETK
                     KARMITLLLIPFVPCTARLAVLTLVSAAIFGQNGAFVAWSLVALNIAVLGIAGVFVNK
                     TLWKQDAPFIMELPIYHTPDAKTIMMVVWTRILSFIRKAGTVILAVSLIVWMLSYFPN
                     GNVEESLLASFGRLLQPLGIPLGLDWKMITALLTGLVAKENVVATLGVLYSVGRDGLS
                     NMLPAIMSPESAAAFLVVMMLFIPCAATIAVLKKEMNNNKWFYTAIVMTLLVSYVGGI
                     AAYNFVKWLGV"
     gene            complement(49239..50411)
                     /locus_tag="PLG98_00610"
     CDS             complement(49239..50411)
                     /locus_tag="PLG98_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOE31778.1"
                     /translation="MKKNILIRNVCRGFIFLFVVIPLLSCSTFTAARYDADVSPGHCL
                     PVFPDRDGWYGGDGAYSIKLDKDRTLWVFGDTFVGCPGCRRDRIDMDIVLGTTVAISS
                     CPENRGFEITYYLQHSGGKYVSSFGKNEWLWPQDPFMVNRVLYIPLISIVPGDKEDEV
                     FNFKVAGHKFVRVKDFSATDPRNWNYDYVDLTHALPPEIAAFAATSVVHDRYVFFYPF
                     YVHVKNDLNVFGNILARIPVDRIDRPAGAIEYYTKEGTWENRLSPEKVKIVLDASVSE
                     LSVRYHEDDQKWIAVYLSTQNKGDRLLYQTSDKLEGPWSEAKPLGAPVPEVDPRSALY
                     DKGNFCYAGKEHIGFSRGKYLVVTYVCNSSEDMEKQDSFIRRNMFLYRPVVREIGY"
     gene            complement(50416..50973)
                     /locus_tag="PLG98_00615"
     CDS             complement(50416..50973)
                     /locus_tag="PLG98_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOE31779.1"
                     /translation="MGFITIFLIATGLGLDAFSVAIGIGAAHQKRSWIPVLRLAIAFG
                     FFQFIMPIIGWMAGLTVVEVIASFDHWIAFGLLALIGGKMILEGCGKGIDETQSDRTT
                     GWPLLMLSIATSIDALAVGFSFSVLKHPILFPAVVIGVVCFVMTATGMIFGKALAKLF
                     GRKVEIFGGIVLIVIGVKILFDHLM"
     gene            complement(50983..52833)
                     /gene="uvrC"
                     /locus_tag="PLG98_00620"
     CDS             complement(50983..52833)
                     /gene="uvrC"
                     /locus_tag="PLG98_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOE31780.1"
                     /translation="MMKENNVLKDKIALFPRSPGVYLMKDPAGKIIYIGKANHLKSRV
                     ASYFTGKDSRPMTPFLMARVYDIEFINTATAKEALILENNLIKRHRPRYNVTLRDDKT
                     YYYLSLDPTDKFPRLQLVRKRLHNAALYFGPYPSGLAAKETLRFVQQIFPLRSCRNRD
                     FKLRPRPCLEYQIGRCLAPCSGRINEEAYRKLADNAVSFLRGRSRELITELKKQMNDA
                     SEELHYEEAARLRDRIAALEHALEKQNVERGEEKDQDVFGFFSRHHHYQVCVLFVREG
                     KLLGSKSFAPIRLKIDASEIVSSCLTQYYDGDVTLPDEIIMPCHLPDEGVIAEWLSEK
                     KNKKMILTVPSIGAKNALLQLANDNARNLWESTYKKREEKEFALQILQEKLSLAGLPR
                     RIECYDISNIGGTYAVGSMAVLRDGDPDKNSYRRYRVTAPAEPDDYKMMQEVLSRRFS
                     SGEHLPDLVVVDGGKGQLNVALSVLKDLKIRTDVIGLAKEKCRRCTVRAGQKTTRTWE
                     DRVYLPGRKDALYLSAWPQALRILQLVRDEAHRFALRYHHQIKHKGDLHSVLDDIADI
                     GAKRRKILLSHFGSAGKIKDATKEELQNVPGIGKVLAEKIFNALKIIQNE"
     gene            complement(52894..53289)
                     /locus_tag="PLG98_00625"
     CDS             complement(52894..53289)
                     /locus_tag="PLG98_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31781.1"
                     /translation="MKYLIITGAVLILLAITTGWFIIAGKYLSLTVMKKIIRDEEKLI
                     KAHVDYVIMGILLLVLFATGINLPLVVAVLACAGALANPSLFLFLAVKPNVNKKAGSF
                     FGIVSTLVFLITTAGIGGAALLLIQELIQ"
     gene            complement(53330..54370)
                     /locus_tag="PLG98_00630"
     CDS             complement(53330..54370)
                     /locus_tag="PLG98_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31782.1"
                     /translation="MSRRLKGFLFILCFLLISFLVAELFFRYLYPKIAPFDDRIDVRY
                     ANPYTMFSRFAYGSQESAKTRGEFRIFMLGGSTVEYGNPPLPVYVEQELKRRGFENVR
                     VFNYGVISQNSGQELAHLVFHVLDSEPDMVVFYDGGNDIMDPLLYDPRPGYPFNFLAY
                     ENNVLFQATGDYPWPALLAYGSEMLRHVFRNYFFEKFGNFSNLRLSAQHGEYAWSEKI
                     AGIYAGNLAKAGQICRAYHVEYVAFLQPIIFYKRFAVSDTEKNILKGWPPEEAGIVRP
                     YTNDVRKRILRNIREEQKRFPFEFHDLSDIFCNSRDAVFTDYIHVNDTSNKKIALAIC
                     DRLMDNIKNSHP"
     gene            complement(54443..56257)
                     /locus_tag="PLG98_00635"
     CDS             complement(54443..56257)
                     /locus_tag="PLG98_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE31783.1"
                     /translation="MSVNYFIVYNPLTYQLMDLMPANSITPFDVITYLSGQNSHEVNA
                     ENYLRFIDFQPEKKRATMVDALAIEMYAGIYIQHQLAKLAQDHLVVMVDGKRRNFSEV
                     IRQKKAPPQAVFISTISSSFPAAAAAAIVLNHAKIPVIIGGIHVSTMPEDADTFIRKY
                     APYPELISIVRGAGDSTVLKTILDDLKTHSLKRDYSGETLIENGVWGNFDNLEPLEPL
                     KINLLNRLPVIKYTPLKDFRINPVAPFTGCIFSCKFCSISTLPQRNKIVQFRDTDDFV
                     EELRSYQKEIVDFKNRYYFFTPDNLFSNKKNLISFLKKIIASDLRINYAVQISIEVAD
                     DEELLKLIRASGGTHFFIGLESLDIRNLKHINKHIVKDIEKSGMSVKKYYASKIRKIH
                     AHGISVHGSFIVGLPYDYFNSPTDNTGVDITKFCIRNRIGAQPSTIVDLPGSVFFHES
                     QENNTFIYGKKGTMDYFLSLSVADLAETNKIPPDSLHQSPLIIASMVYEISKIVGAPV
                     RATFNSSIIFFRSLLHPTNKGQGLHFWQRFIDAVCSAVSQTAVALYKEQGESLVRSKH
                     GVRGIMERLYDTEKNPEIRKMFKNYIPQFTEYGTSGKT"
     gene            complement(56508..57140)
                     /locus_tag="PLG98_00640"
     CDS             complement(56508..57140)
                     /locus_tag="PLG98_00640"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HOE31784.1"
                     /translation="MPVRTYHLILLATVLIVWIWSAIKPQDTYLTWFLETLPFMMALP
                     IILLTYKKFRLTDLTYTLIALHAMILMLGAHYSYAKVPLGLWMEEWFGWTRNNYDKIG
                     HLMQGFGPAIYAREIIARTSPVQKGKWLAFFSLAVPLAFSALYEILEWAASLSNPNDT
                     EAFLGTQGYLWDTQTDMFLCLIGSMASLLLLATLHDKHLLKTQAPGINKK"
     gene            57319..59301
                     /locus_tag="PLG98_00645"
     CDS             57319..59301
                     /locus_tag="PLG98_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOE31785.1"
                     /translation="MKKIFLQFFLLLVLFGLLPVAGIFNPVAAEESARNASGNQTEID
                     LMLLFTNDLHSYFLPHRVLTEDGRQTVQGGYARLACMINEQRRLHPAKTLLVDAGDFS
                     MGTLFHTAFMTEAFELRLMGKMGYAATTLGNHDFDFHPEGLAKMLTAARSKSQILPQI
                     VASNLIVNGDHPGDKTLKQALNDYPVRDYMVLERNGLRIGLFGIMGKDAADDAPFAKP
                     VMFADPVEASKKTVAILKNREKADLVICLSHSGTSADAKKSEDEILARAVPQIDVIVS
                     GHTHTVLKTPIIIGRTIIVSGGRYGRYLGSLGIRFDRFKGASLASYDLRHVSSEIPED
                     KAVQEAIAGYEKIVNRDFLAHYDLTFEDVIAESDFSMESLESMHRHPRETGLGNLIVD
                     AYREAVKQAEGPDYRHVHFALQPLGVIRDSLLKGKITVSDVFRVLSLGIGPDRIPGYP
                     LVSFYISGKELKDILEVHTTIAPMHKKDAYLQVSGVKFTYNPRRIWFDRVTSVWVADE
                     KGVYQPLDQTKLYRACANLYTAALIDYVSSVSFGLIHVVPKDSSGKRLTDLNSSIVSM
                     KKTGEIKEWLALALYLRSFQEPQHLQTAKIPLKYSKPEGRYLAQPSCNPVRIFSKGNI
                     LTKVFLVSVILLLMICGLIVYVFVKKMKKISKKNPR"
     gene            59552..60298
                     /locus_tag="PLG98_00650"
     CDS             59552..60298
                     /locus_tag="PLG98_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOE31786.1"
                     /translation="MQIELTPVEKMKAPEQIAERLLKYILGGGVSPGEKLPPERTLAT
                     QLNVTRATLREALKKLEQLKLIVIHQGKGIIVEDFHKASIDLIFSLLVINGQIDLKIL
                     ENILEARELFGTDVARCAARRADKKDVEQMKILMNELIKATDPGKLQQLDFEFFRQLA
                     LASKNMVYILLMNTIKTIHDNHLDLFLPLSTNLDTTLQQEILQAVIDGDEEKAAEKAN
                     TFLKAGMELLKFLKSLSDTKESQNADRETN"
     gene            60276..63581
                     /locus_tag="PLG98_00655"
     CDS             60276..63581
                     /locus_tag="PLG98_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOE31787.1"
                     /translation="MQTARQIDKDPIEQTQKLNVLKIQRTCVHDGPGIRTTIFFQGCG
                     LRCVWCQNPEALSLKPMPSPAVHYAIADVLEVVLRDKDYYLKTGGGVTLSGGEPLLQD
                     PESLTHLLKKLKDEKIHIAVETSLHVPWENISKTAPYIDLFLIDLKVVGDEDLHRKCT
                     KQDSRLIHDNIRKLLASKANIKFRMVMVPEYNDHEQHIKATADFLKSVNHKSIELLKY
                     HNMYEDKAKRLGLVRESLNISPEQSLTAVKKAVELLKPLDIYAECEDLGNFRSKAVFT
                     DRVHAIQKDIRKSGYHLCFETSKLKTKFYRKNGFKKPTHIHRAERLDYVLKNKQVRIY
                     PHELLVGNFTSKRVGGQVWEEHYGILFSSILHQIHRQTPVAFQCSWKDRLYFYCRILP
                     FWQKHCLLTKVNKTLSDLVLSISRLCEMNVGFNNNMAAIAHFIVNFERILTLGTTGII
                     KELEEAQKEKPQNDQTFYQGAIIAVKALENFALRYADALSDMSKTEANPVRRKELEDL
                     VQICRHVPKHPARTYHEALQSMMFLQVALCIESYENAISFGRLDQILYPYYKKDKEAE
                     IITYEKAKELLALFILKMDEAILVNDGDTYFAIGRLFETQSTDQTVTFGGVDKDGQDA
                     TNDLTYMLIDICELQFLAVDMSARIHPNSPERYLNRIAEVYINGSPQPKLNNDEVYIQ
                     SLSKHYDTSIENIRNYSIVGCVEPNASDDHFGNTDCANMNVVLPLLQALKGEEDDLWN
                     YGFSDQMEKLTTKFIEYNFRGGNRVSKWIADKYGKARASYKTKKKTAYNPPANMDELL
                     ERYQNRLNHLASSILADHQKIEKEICKNFTTPLASALSRGCVEKGLDVNQGGCEFNTS
                     GIQAVGITDAADSLHALNEVVFKKKMYSLMDVIHAIDNDFEGEEDEQIRQALLAVPKL
                     GHDDSPEAVKWVNKTLEIYCNALRQVPNSPRNGFYTAGYYALNVSDVYGKKTPSLPSG
                     RLKGVPLANSISPHYGMQMSDLLSSLNSVSGVDFVKYAPNGTTVTFTMDSALFQGRDG
                     ITNLAGIFSTYFKKGGMQFQPNVINREMLLDAYNHPEKYPYLLVRIAGYCAYFNHLSD
                     DLKKVIINRTCYS"
     gene            complement(63660..64307)
                     /locus_tag="PLG98_00660"
     CDS             complement(63660..64307)
                     /locus_tag="PLG98_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE31788.1"
                     /translation="MLKKYRYVLTDFLQDFLYYFIRGYSWTFRLQVVNEKPWMDHLKQ
                     GGRILICCWHQQFFSAIRHFKTYAPYHPALMISQSKDGNIIARIAEKTGWHTVRGSSS
                     KDGSRALRGMIDYLIQSRFAGHVVDGPRGPAGIVKAGVVKLAQASGALVVPFYISADR
                     AWYFNSWDHFMLPKPFAHVTLRFGDMLDLASGESEEDFERQRLRLEGIMKPNLHL"
     gene            complement(64451..64918)
                     /locus_tag="PLG98_00665"
     CDS             complement(64451..64918)
                     /locus_tag="PLG98_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31789.1"
                     /translation="MKTIKIYCIVILAALLLSAYGSLAADWGFRGLLPRGTGYYDKNS
                     IRKISDHIYQVWTVTIYNERGRADAYAMLLKQEKAPGNPAVLNQESILLELDCLQGKY
                     RIVSINIYDEKDTLLFSVPETNDGWHDIIPNSINEKLNNAVCKAVDNHRNGNP"
     gene            complement(65004..65885)
                     /locus_tag="PLG98_00670"
     CDS             complement(65004..65885)
                     /locus_tag="PLG98_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE31790.1"
                     /translation="MKEETEINIPKEFTNKLFWQSLNPMSITRATDGMYVDVNESFTE
                     FFGRRREEVIGNTAADIAYATSLERLKILEEINEKGFARNITITVRDKKGDLRCALVN
                     TLPVEIKNQVYWLTVGTDISDLNPAAKISHEDALIKSLNSIKGAGVVLIRNHQKKQPS
                     LCYMNEEAKMVLKTHSLDNLLRTLAENDSTYLSIGAKYYHVKNINNHRRSPLKLIVME
                     RMPDTICIREKLTILDLTPRQKEIAFLAATGYSNREIAGRFCITEYTVKDHLKKIFHT
                     LGVKKRSEISPKLFHWR"
     gene            66294..66686
                     /locus_tag="PLG98_00675"
     CDS             66294..66686
                     /locus_tag="PLG98_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31791.1"
                     /translation="MKKRIMVLLSIIVFVLGLVCIQGCQQNENPKFQNEYQAVLLTTG
                     QVFFGKVEFVGTDYVLLKDVFYIRSVANPDTKQVSNTLIRKGNEIHGADQTYINARHI
                     IMIEPVSPQSPLAGRIKELQSQKPQGQP"
     gene            complement(66801..67985)
                     /gene="metK"
                     /locus_tag="PLG98_00680"
     CDS             complement(66801..67985)
                     /gene="metK"
                     /locus_tag="PLG98_00680"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOE31792.1"
                     /translation="MSKLHTRHIYTSESVSEGHPDKVCDQISDAILDACLEQDKHAHV
                     GCETAVGPNLVVNLGEITCKNWNKINPQAISRQVVQDIGYDDPCENFSWDTFNYLSAI
                     HGQSPDIKQGVDRDRPENQGAGDQGMMFGYASYATPQMMPAPIYYAHLLLEELTILRK
                     ANVIDYLRPDSKSQVSVLHEDGKAVKITTVVISHQTLDVPLKRIRKDLIDVAHQVLDK
                     TGMLTNKTEFFINPTGRFVIGGPAGDSGVTGRKIIVDSYGGVGSHGGGAFSGKDPSKV
                     DRSAAYYARYAAKNIVAARLADKCEIQIAYAIGVAKPLSINVCTYGTSVIPENKIEKI
                     LTEGKIFDFRPAMMIKDLGLTTPKGWSYRKACNYGHFGRKGFPWEKTDRVDALRQAAG
                     LK"
     gene            complement(68459..69019)
                     /locus_tag="PLG98_00685"
     CDS             complement(68459..69019)
                     /locus_tag="PLG98_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31793.1"
                     /translation="MKKLMSGIIFFAGVLFAPFSVSAGVSVHIDIPLPPPIIFPAPPH
                     LVVIPETYIYAVPDIQDDLFFYSGWWWRPWNGRWYRSRYYDRGWTYDHQPPFFHRTIP
                     PGWKDNYRNKTWKGHRWEHRKISHHELQRNWSGWKKEKRWEKHSWGVKNWKDRRSGTQ
                     YRREVKKSKTVIKYRDDRRPVREDRR"
     gene            complement(69174..70115)
                     /locus_tag="PLG98_00690"
     CDS             complement(69174..70115)
                     /locus_tag="PLG98_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE31794.1"
                     /translation="MRLLKAIVVLIILCVATGIGFVYLAPEQATSLALDVERKRSGLE
                     KKEINLSDHIRYVYLEGGKGEPLMLLHGFGANKDNFTRVARFLTPHYRVIIPDHIGFG
                     DSSRPQNEDYGSLAQAVRIRTLAQALGIKDVHVGGSSMGGHISMMYAFLYPDEVKSLW
                     LIDPGGIWSAPPGELHEIIAKTGKNPLMAKNEDEFAEIFSFVMTDHPFIPRPILNVMA
                     QERIRNYELEQRIFKQLSSDASERYVKEIKTPTLIVWGEKDRVISPQTAPILNKLLQN
                     SEIIIMKGLGHLPMIEQPKIAADDYLKFRKRLAKQGS"
     gene            complement(70194..71525)
                     /locus_tag="PLG98_00695"
     CDS             complement(70194..71525)
                     /locus_tag="PLG98_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOE31795.1"
                     /translation="MSIWNKKNTIVVTCPKGVSPFLKEEVQALNYPVRAEWDTAIQTE
                     GTLQDTMALNLHLRTAQRVLYQLDSFSADTPEWLYKKIHALPWENLLHDSGPAAYLCV
                     TSIAENPLIKDSQFVNVKVKDAIVDRLRDISGNRPDSGPEKNRAVVHVYWKDNRATVY
                     LDTSGERLALRGYRKVPLVAPMQETLAAAVIMAAGWKGESNFINPMCGSGTLAIEAAS
                     IALHRAPGLLRNHFGFMFIKGFPEDHWQTLRSKARTNALKKLPARIIATDINREAISA
                     ARQNAQTAGVDHFIEFNICPFEKTSVPAGDGIIIFNPPYGERMSSDPRKPVSADFRHS
                     RETPGPGRKIILRKSGDKSGDGNRHRGREMKKLEALYQGIGDFFKKTCTGCHGYIFTG
                     NLELIKKVGLRTKRRVILYNGDIECRLLEYALYTGSLKNTAKEKSLSAENG"
     gene            71704..72948
                     /locus_tag="PLG98_00700"
     CDS             71704..72948
                     /locus_tag="PLG98_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE31796.1"
                     /translation="MKNDRKIFWGWYIVGGAFLVMAMNYGARYCFGVFLKPMANEFAL
                     SRSVISLAAAINMMVYSFGSVFVGRMLDRVAPRWITTAGAGVAACGYLLTGFVDTPIW
                     LYISYGVMVGLGSAGMGVVACSSSVSKWFISKRGVAVGIASMGISLGTVMLTPLAGYI
                     ATVFSWRFGLMALSFIMLIVGVVVAQTLMRRTHPEAYGLLPDGNTISGMPQAAEATAV
                     CPVSTMILFRDSRFWTLAVCQGLAVMISMAVFVHQVAYATDNGISKMAAASSLAAVSL
                     TGFMGQFFFGWITDRINDPKYASFLGISFMLAGTVILLNVESLLHLYVAALVYGFGYG
                     SLAPMLPLIIADRFGRQCMGSIYGLLTFFIGTGGAIGPVLGGFIYDHFGSYHNLWLMN
                     IVVLSLIAMAFLTLKKGRPYAA"
     gene            complement(72966..74129)
                     /locus_tag="PLG98_00705"
     CDS             complement(72966..74129)
                     /locus_tag="PLG98_00705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE31797.1"
                     /translation="MQNFVFENPTKIIFGKSQIPRIGKETARFGRRALLVFGMGTILK
                     NGVYDQVTNALEKAGVEIVDFPGVKSNPVLSHVQKGIALARGENVDVILAVGGGSVID
                     AAKAIAAGVVAHHDVWDFFTFSKPVTGALPVLTVATVSASASEMNAGAVVTREDGCQK
                     YCIVSPFIQPKVSVLDPTVLFSLSPAYSAYSAVDIITHMLEGYFNNTSSASPLQDRLV
                     ESLIKTVMESTEIILKEPDNYDARANMMWSAGLAFNGLTTAGMGQVRMPAHMIEHSLS
                     AIYDIAHGAGLSIILPAWMSYALDKKTDRLEKLAREIFHVKGRGAISAAKKGIACLKH
                     WFESIGSPVSLQAVDIPESDIDAIAGNAFALSQVWCYEGYTKDVIRKILLLAR"
     gene            complement(74197..75426)
                     /locus_tag="PLG98_00710"
     CDS             complement(74197..75426)
                     /locus_tag="PLG98_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31798.1"
                     /translation="MNGSNFQIEQQCPQCGAPIILDETDRILSCHFCRTRVYLATDDH
                     FLFYIPPAAGISEKIYFIPYWRLKGLSYAITTHNIAYKYFDANFISFDLPFFPHSLGL
                     RPQAMKLRFVGNADHEGKFIAASQKTRGKLLKNFKTQAGAVQEIFIGETASIIYTPVY
                     FANGRLYDAVLKKPLDIQKKEPEMEDKLVSSPDEKWHVDFLPLLCPECGADLPGAKDA
                     LIVFCENCHRAWNPSKKHFVPIPYATWREKGDEITYLPFWQLKVKVDGIALGNYADFI
                     KVANLPKAPLDIWYKTPFYFFAPAFKLNPALFLRWCRQLTVTPAPDDLTPDCPVKNIH
                     QVTLPVSEAFESCLVTLGSLMADKRHLADMLSSLKLTLEHYSLVLHPFAVRNRELVHT
                     KLDFSMDISALTMGAFL"
     gene            complement(75413..76531)
                     /locus_tag="PLG98_00715"
     CDS             complement(75413..76531)
                     /locus_tag="PLG98_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOE31799.1"
                     /translation="MGTNNIIFLEVIEWFDESGKEIVHRIPEKGSGEIKFGAQLTVRE
                     SQSAVFFYKGKAYDAFGPGRHTLKTANIPLLTKVIAIPWGMTSPLRAEVYFINMKVFP
                     NLKWGTRDPVAFRDRELGLIRLRAFGVFNIRVIQPLLLINSLVGTQGIFSTEEIEEYL
                     NRVIVSRFNDYMGEKVDTIFNLPGKYNEFSEGLKVMLQKDLSKFGLALTDLYINSITP
                     PPEVQKAIDDKSRLGVFDDLNKLLKMKAAMAVEAAAQNQSEAGAGLGMGMGFMMPGML
                     LEAFKGAGTTVAADVFTCPDCRQVVPKEAHFCPACGHQILIVNKCAHCGRNLPVNANF
                     CVSCGKPVTVNPPGKQCAKCGFENLPDSMYCNKCGERL"
     gene            76678..78105
                     /locus_tag="PLG98_00720"
     CDS             76678..78105
                     /locus_tag="PLG98_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE31800.1"
                     /translation="MKKTSAEQNIYDGLALIADPIHQYILFTVPKDKSAKSAGEITEQ
                     HIIDSPWMQRLRRIYQLQSARWVYPAAEHSRFQHSLGTMHMAGEFAKSIYPSLSSVCK
                     DVPSCHYVEELLRLAGLLHDVGHGPFGHFFDDNFLSNWSLTHEIVGQNIIVDKLGDDL
                     ARVRRSPSGGFSRTERMDAQKVAYLIRMPEDGEEGRPPRWLQMLRQLFSGVYTADNLD
                     YVQRDAYMTGFSLDMVDIPRLRYYTFFTREGLTLHQSGISALRRFLNARLNLYTNVYF
                     HRTTRGLDLHLQEFFHDTMQMILPANPLNNLDDYLQCDEWTLFNHVQNWLRSKDEQKK
                     KLAAEWKKLHDREVKWKMSFVAEISLDQIQPGVGFSRAADYEQQIRERLPKPLKSLPF
                     KVDLATQDPRPLNPITEREKRINIFNPVTHKTSPEPLLEIYRFIPARVMQFRVFALNH
                     DHDGDLAAAAEKVLGSSAKSTLTNI"
     gene            78107..80167
                     /locus_tag="PLG98_00725"
     CDS             78107..80167
                     /locus_tag="PLG98_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOE31801.1"
                     /translation="MSKNDETTDLTLFVRTTGEEIVLWDRQRIVDALVREADIDDDVA
                     GQISKEVEKQIIVSGIGLLTTALIRELVNVRLIERGLEKERRLHGRLGFPLYDVRQLI
                     LHPNKQDANIPHSPEGTNLIFAEGIKRELSLFDVFSADVGEAHATGDIHIHALGYIDR
                     PYSCCQSLEYLKINGLNLPHAINSANPAKYAEVLLAHMVRFSAVLQGHFAGTVGWDAV
                     NISFAPYLASMTDKEVKQFAQMLIYEFSQLAATRGGQALYTDIHIYYDVPPHWADLPA
                     VGAEGKPTGKTYGDYTWDAQRFAMAILEIFAEGDARGKPFILPRPLLHITDHVWKNDK
                     AREFLLRACDVAALMGNPCFVFDRDEKAVDLIFNDEERRKPWLQRRAAIQTVTLNLPR
                     LGYKTGGDEKEVFSLLEAFMEKAVKAHVQKKDFLEKLLSYAEEGPLAVLAMNHDGFPF
                     LRMHLASYVIGIVGLNELVQIVTGNEMHQKQEALEFGLRVVDFIRQEALRLSRQCKMK
                     FVLGQSLAETTAYRFARLDLRYFSPTAGRYVKGDIAEGAIYYTNSTHLNVAADVGPLQ
                     KVMREGLFHHYLEDEVITHLQLGDLNPGRNVLARFIRDVFYHSANRQIDFNPEFTFCL
                     SCKETTRGLQDKCAACGSADVEGIARLTKYFSKTSGWNKGKLAELKDRTMNDNFNNVD
                     QPKC"
     gene            complement(80164..81087)
                     /locus_tag="PLG98_00730"
     CDS             complement(80164..81087)
                     /locus_tag="PLG98_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE31802.1"
                     /translation="MKKILLKIINPVVLFNAWLYHKKNSTFSNSTSDLELHLYSKIFK
                     NDMLHYGYFENTEIEVDTISIAEIEKAQVRYAQNIIDQIINPQDLILDIGCGMGGLSN
                     LISQKGFNVEALTPNKNQIRHIQSKYPTITCYQKRFQEFETDKKYGTIINSESLQYIP
                     LEKAFEKAESIVKPDGTWIIADYFKFDNSGVNKSSHLLDDFVRKSKEHGWEITYQQDM
                     TPHVLPTLKFVYNYAERFLLPLVHFTSGKIKRKKAWLYYLIRDLKQSVDHKIWKELAA
                     VDPIKFRNEKKYMLFVLRKNCRDAADNPASS"
BASE COUNT        20359 a        19333 c        19898 g        21678 t
ORIGIN      
        1 aacaagtaaa tccagcggac gccgtaccgg cgccgctgat ttctgacgtt gggcaggtaa
       61 ataaacatca aggaggagaa ttatgggctt tgcaatcgga caacttatca gccaaataat
      121 tggaggtgct atggctgcgc ttctgatagc gcttattatt cagtgggcta ccaaaaaagt
      181 ggccaagttc aagccctctt atggaatggc atataaaagt gcattccttg gaactgttgg
      241 aggcttaatt ctcggtttta tgcttggttt tgcttttgga atggctggtg ctggagaagc
      301 acagcaaggc ggattaattc tattaagcat gatagcaggg ttccttttac agggcgcaat
      361 atatggtcaa ctactaaaag atgcatcaag catatccgtt ggcctcggta aggggtatgc
      421 aattgcaggg attcagtttg caattggaat aggaattttg gttattatta ctggggccgt
      481 acttgtaatt gcaagctgaa attttttctc catgcgaggt ttattttgaa aaaagtcgtt
      541 ttattcttga tagcaatatt tttccctagc tattgctttt cagcgaatat aaaaattgat
      601 tcagaaataa tttccgtacc agcatatgcc ggctttgttg aaatcagtaa aatttcgcca
      661 gacacaatgg caatgttcaa agatatgttg ccaccaacaa acaatctaca tgctgtcttt
      721 gtaacggcat ccgattctgg gaagttgcta aaatctcaat cccctgaatt gaaaagatac
      781 ctcatcgtcc aaacagccaa gtcacttgag tctgttgctt ttggtgcacg ccattttgca
      841 gaactgcgta ctaagatccg ggaggaatac gaaagccttt ttgaccaaaa caaatctaag
      901 attgacgaat tagccaaaaa tgccagtcaa aaattatcaa atcgtatttc cgacaaattt
      961 gaaatgaaag tgggagaaat gcttcccatg tcgatcaatt ctgaaactgc atatcatatt
     1021 tcactttcat ctcttactaa attttcaact tcaacggcag acgaaaatac agactttatt
     1081 tcagcatgca ccatgtcaat ggttttactg aaaggccgcg tcgtatactt aaatgttttc
     1141 tctacataca acaaccttga agacctgaaa tggactcaaa gtgtcgctga aaaatggaca
     1201 gaatccacag ttaggtcaaa tcaaaatact caagctggat tcggggaaat tgtcccagct
     1261 ggtaccagaa ttgaccctga agtaaaaaaa gttctctcgg cggagaaaaa agcatattca
     1321 acgcttggtc atccaaaatc acagggtttg aatatatcag tcaagtaccc ttccgtttgg
     1381 gtggctaaag agggccaacg cccacatatt gtacaaaaat tcacggggtc gtctactgga
     1441 agtatcgttc ctggatgtat gatcatcatc caagaattgc cctcaatcgg aaaaatattt
     1501 ttggatgggc agatggcgga ggaaatcttt gctgaaggag ttaaagatta tattccacct
     1561 ggcgctgtat atattgatgg agggactacc aaaatagatg cagagcccgg ggcttgggta
     1621 aaatattatt ttgaacaaga aacttcaggc gtgaagattg gcatgtacag tttacagtac
     1681 attctttttt ataagggttc aatgtttgcg ttgcagtgct caatcgctgg ttctgcatct
     1741 gaaaaactta ttctcgaaga cgcgttttca tcctatctgc cagtatttca aaatataggt
     1801 agcagcattt atattcctga caagtggaat aaagaaatgt caaatactga cgttgcttca
     1861 tcggttatga aagataccta tggagaaaat tggttattaa cgctcttgtt ttcggccatt
     1921 cttacatggg ggattggtct tgctcctccc ttccttatcc gttttgccat cattaaacat
     1981 ccgttatcaa agcctcctgc tttgataact gtaatatttc tttgggtatt taactttgtt
     2041 ttttttacag cacttggcag tgaaagcaaa acgcatgctg ggctactact cgttgcatgg
     2101 gcatcttatg ccattctgag aaaaggatca gcaaaatacg aagcagagca aacaaggcat
     2161 cgggaagaag aaaacctgaa agccgaagaa cacagaagaa aggaagaaga gagaagacgc
     2221 gcagaagaag aatcacgtag aaaaaaagac agagagaacg aacaaaaaga aaaatataat
     2281 cgggaacgta aaagcataaa agacgaagaa tactaccaga atattctcgg ccttggtagc
     2341 aactttacag tcgaggacat aaaacgtcgt tacagggaac tcgtagccaa atatcaccca
     2401 gataaggtaa accacctcgg agagaaactg aaagagacgg ctgaaagaga aatgaaagaa
     2461 ataaatgaag cttatgaata cttcaaaaag aaatataact tcaagtaaat gcccaacaac
     2521 cgcatcaact cggactggca attccgctgc gctccattgc cagccggtta tgcggagcgt
     2581 tggccagttg aaatataatg aggaagctgg tacatgaagc ccatgaatat agatgacata
     2641 ctgaaggatt taactgagcg tgccggctcg gctgtggccc actattggaa aacgcgtgca
     2701 ggacagaggg acaagcagag gcttggcgga acggccgatc aggggctgcg tagtgctgtg
     2761 acaggcggcg ctcagatgaa tggttttatt gaccttttta cggaatttat tgtcgcggtt
     2821 ggcatacccg agcaatgtgt atttcgtaaa cgggctgttg aattgccggg attctttcgt
     2881 cctaccaaag aatgggatct gcttgtcgtg cgggaaaaca agcttcttgc tgtgatcgag
     2941 gcgaaatcgc aagtcggccc ctcattcggc aataacttca acaaccgaac tgaagaagcc
     3001 atgggaagtg ctcttgatct gtggacggcc taccgagagc gagcgtatct tgatagtccc
     3061 cagccttttc tcggttattt cttcatgctg gaggattgcg atgcctccaa tagggccgtg
     3121 aaggtacagg aaccgcactt caaggtgttc ccagaattcg tcggcgcttc gtataagctg
     3181 cggtatgagc tattctgtcg gaagcttgtg cttgaacgcc attatacagc aacagcgttt
     3241 attgcctcga cacaggatga tggcatcctt ggccattttt cgacaccagc agacgacctt
     3301 tcgcttgtgc gcttcgccag agtccttatc agccatcttg cggcatttgt gtaacggcat
     3361 ggaagaaacg actcatagac tgattaacgg tgacgcgcgg gagctatcct tcctgcatga
     3421 tgaatcagtc catcttgttg ttacgtctcc gccctattgg agtctcaaac gctacaacga
     3481 acaccccgat cagcttggac acattcagga ttacgaggca tttctgtttg aactggaaaa
     3541 ggtctggcgg cacgtctacc ggattcttgt tcccggaggg cgtctcgttt gtgtcgtcgg
     3601 ggatgtctgt gtcgctcgac gtaatttcgg acggcacctt gtctttccac tgcatgcgga
     3661 tatctgcgtt atttgtcggc gaatcgggtt cgataatctg aatccgatta tctggcacaa
     3721 gatcgccaac gcttcatacg aggtctccaa cggatcgaag tttctcggaa agccctacga
     3781 accaaacgcc atcatcaaga atgatatgga gtttattctc atgcagagga aaccgggcgg
     3841 ttatcggcaa ccgaccaata tgcaaagaga tgcaagccgc attgacaaag aagagtttaa
     3901 ccgctggttt cagcagattt ggaacatcac cggcgcgtcc accaaacagc acccggcgcc
     3961 attccccctt gaacttgcaa cccggctcgt acgtatgttc tcattcacgg gcgacacggt
     4021 gtttgatccc ttctgtggtt cagggaccac gatgattgcg gcactgcgaa caggtcgtaa
     4081 cagtatcgga attgatatcg atccagaata ctgtcggatg gccgcacggt atttgaaggc
     4141 ggagaacgcc gacctgtttg caaccacgaa acttgtcttt gagaaagcta ccgccgaacc
     4201 tgccagcctg gtgaaggaag accttgccct ctacgaagtg cgaccggcga gaaagaaatt
     4261 ggcataggcc aacaaaccca tgcaagcgac gcctgaaggc gcgcctgatg ggtagcgtta
     4321 gaaaataagg atcagatatg gcttttagtg agatagaact gaagagaatc gagaaagaag
     4381 tgggcgattt ttgtgaaaag cgaagtcctg ttgaaattcg tgataaattg agtttccaat
     4441 atcgtattaa agcacacgat gtcatcattt atacgcgccg tccccgatgg gataatccca
     4501 aagaatggat ggaaagtgat atggcaaaaa ttaagtacgt gcgcacttct aatatgtggc
     4561 agttgttttg gcagagggcg aatggcaaat ggtttagcta tgagcctttt cctcatagta
     4621 aaaatctcaa gtcaattatt gaagagattg aggcagatca atacggctgc ttctttggct
     4681 aataaaaatc gcataaccag gcgctagagc cgatcgcttg cgctccggct tgttttatcg
     4741 ttagacgaaa acgataaagg gagatatcca tgaaaaagat gcaaatggat aaagcattta
     4801 cgctgatgga gtccggtccc gtcgttctca ttacaaccaa tgacggaaaa aagaataata
     4861 tcatgacgat atcgtggacc atggtggtgg attttacacc ggtatttgcg ataactaccg
     4921 gatcatggaa ctattccttt gccgctcttc aaaaaacgaa ggagtgcgtt attgccattc
     4981 ccaccgttga catgcttgat aaagtcgttg gagtgggaac ctgttccggc tccgatacgg
     5041 ataaatttga aaaattcatg ctgacccccg tcaagggaaa gcatgtcaaa gcgcccctga
     5101 taaaagaatg cctggcgaat atcgagtgta aagttgccga tattgtacga aagcacaaca
     5161 tcatcgtgct tgatgccatg gccgcctaca tcgacgataa gcggaaagag aaacgagcgt
     5221 tgcacgccgt tggcgatggc acgtttattg tcgacgggcg taaaatggac cggaggaaga
     5281 tgatggcctc caagcttccc gcaggcgtct agcttgtgtg attcgcctca ctggtcgcgc
     5341 cacgcaccgg ctgaactttt tgttaacggg gttattgaca tgaaaaaatt tagaatttac
     5401 tgctctggtc cgctgttctg cgcagaagaa atcggcggta tgagtgccat tgccaaagta
     5461 ctggacgatg caggctttca aaccttcttg ccgcatcgtg acggactgga atcgtatatc
     5521 ctgcgttttt caaatgcatc gtttccatcc accatttcag cgatacggac acgggttgat
     5581 tacgctatat tttcattgga tgcctatgaa ctgattgaaa gatgcagcgc tgtcgtatgc
     5641 aacctcaacg gccgtgttcc ggatgaagga atgattgttg aagcatccct tgcatatgcc
     5701 gccggcaagc ctcttgtctt tttcaaaaat gatctcaggg ctccttttgg cggatacgat
     5761 aattccatgc tgacgagtct ggtgcgcgga aaaattgtca aaaggctgaa ggaattgcct
     5821 tctgcgcttg aatatgaatt atcgcgggaa ccggaattaa tatccctgcc gggcggttta
     5881 aaaaaagcca tctcatatgg gaaacgtatc tcatcggtat tggaaaactt acaggataag
     5941 atattcggga aaaactcgcc ggacgaaatc gtcagccagg ttatggaaga ggcgctcaaa
     6001 gagcactgat cgtcgctaat ccgccggacg gcttgcgact gctgatcggc acgttagggc
     6061 ttccaccttc cacgacatca aaaaatgaag tttttccggg tttttcaatt tttcgttttt
     6121 cctgtttaaa aggttttatg tctcagcaaa tattcagtct ttcctcctgg ccgcgcgcga
     6181 ttgtgcacat tgacggcgat gcttttttca cgtcctgcga ggaagccatt catccggagc
     6241 ttcggggcaa agcgctgatt acaggcggcg agcgcggtat tgtcgcctgt gccagttatc
     6301 cggccaaaaa actgggcatc aagcgcggcg tgcctctggc tgaggcgcgt aaaatctgcc
     6361 cgggattgat tgttctgcct tcagactacg aaacctacag cttgttttcg cagcggatgt
     6421 tcaacatcat gcggcgtttc acgcctgacg tggaagaata ttccatcgat gaggcgtttg
     6481 ccgatcttac cggcatgcga cgattgctgc gttcctccta cgaggaaatt gtactgaaga
     6541 tgaaaaagga aatcgaacgc gaactgggca tcacggtttc tgtcgggttg agtatcacca
     6601 aggtgttggc gaaaattgcc tccaaacatc agaagccatc cggggtgacc atcatcaagg
     6661 gaagggacat tgcctcttgt cttggaaatc ttcccgtgga gaaaatctgg ggcatcggtc
     6721 ccgccacaac caactatctg gcgaagatgg gcatccgcta cgcgttggaa tttgcacaac
     6781 tgccggaaaa gaaagtgcgg gaaaaattta ccaagccggg cgtggaaatc tggcaggaat
     6841 tgcgcgggga atcggtttat ccggtgacgg cagaggagaa aagttcttat cgttccatca
     6901 gcaagacgaa aacctttgcg ccgccgacgt ccaatgccga ctatctgttc gcgcacctca
     6961 tgcgcaatat ggaatcggcg tgtatcaagg cccggcgcta ctgcctggcg ccgaagaaaa
     7021 ttgttgtctt tctcaagaaa aacgatttca acaccgtcgg cagtgaagtc aagctgtcac
     7081 gcccctgtgc ttttcccatg gagttttccg gcgttatccg tgacctgttt gatcaatgct
     7141 actcggtaca ggatatctac cgcgccaccg gtgtcgtttt gtgcgagctg gaaccggacg
     7201 agcaggtgca atattctctt tttgacaatc ctctgcaggc ggaaaaaatc agaagcgttt
     7261 acaatgttgc cgatgaactg gggaggaagt tcggcaaaca tgcgctgcat ctgggaagct
     7321 ctcatcccat cgatacgttg gggagaggcc gccggggtaa tccgaccgtc cgtgaacaaa
     7381 cccggctaaa aggcgaaacc cgccgccgtc atttgagtct gcctctgctg cacgtcaaga
     7441 catagagaat tttatgtgaa cgtaacggat tttttatcag aggttcttta tctcgtactg
     7501 ataataaatg tcgtcatcac cgctggcgat acttctcacc cttttctcaa tattttcttt
     7561 cccgaatatt ttaagggctt gcttaaagta ccccatgatg aggtactcga aataaatatg
     7621 cttgatggga atatcgctga ttttaagatg aacgatgtta ttttccaact ttgcggtaaa
     7681 cttgccgccg tcataatagg tggaccaaag tttgggcata cccgattcga cgaactgttt
     7741 gatgtctttt gtgagaagat aagaatgata gggcccgccg ggagacaggg cgaattttgc
     7801 cgctaccctc ccaaacagta aaaactggct tttatcgttt ctgaagaact ctttcagcat
     7861 ttcgtcaaga aaaaaaagat gcttgtctac cggaacaagc gtgacagaca tgataacatt
     7921 tttgaaaaac ggatcctttt ccgcaagctt ggccatgaat tcattccagc gcgcttcgcc
     7981 gaaatttgct gtgatagcga cttttcctgt cagaaaaaca gtacctttta cattcatatt
     8041 aaaccctccc cggagtcgtt gattggctta aaaataagat gagtgatggt cacgaatatg
     8101 taatggcgtt ttttcatcgg aagcaattaa ttttcgcatc atgccggatt cttacgcttt
     8161 accgttacat caaaggctgc gtcgctggac gccttgcccg tgacagaaac cgtacttttg
     8221 cgttcatgtc accttccaaa gtatatgtgt atgagttaat ccttatattt tgtgttgcat
     8281 aaatattcaa gtacaatatt atttttttcc cattgcgtct tcacattttc cccttttgaa
     8341 aaacataacc ccatgatatt ataacttaga gttatttgaa gaaaggaaaa tttatgacat
     8401 taaaagacat taaaaaagta ctcattctgg gagccggcac catgggacag cagatcggtt
     8461 ttttatgtgc catgcacggc tataacgtga ccatgtatga tatttcggaa gacattctga
     8521 aagcctctgt cgcgcggatg gaaaaactcg gttcattatg gtttgtccgg atcaacagga
     8581 ttacgccgga gcagctcaag gaaatcatga gccgtatcac agcaacgtct gatgcgaaaa
     8641 cagcggccag gaatgccgac ctgatcagtg aatccgttcc ggaagatccg aaactcaaag
     8701 gcaagatttt tgctcaattc aacacactct gcccgaaaag gactattttt accacgaaca
     8761 cctcgctgct tatgccgtca aaatttgctg aagccaccgg caggccggga aaatttgccg
     8821 cgttgcattt tcatgatgtg cgaacaacca atgtggtgga tgttatgcct catccgggaa
     8881 cggataaagc cgtgaccaca ctggttcacg attttgccgt cagcatcggc cagattccca
     8941 tcatgctgca tcgcgagagt aacgggtatg tgtttaacgc gatgctttcc aatcttttca
     9001 tgacagcctt aaacctggtt acgaaggatg tggcaagtat tgaagatgtt gatcgcgcct
     9061 ggatgggcat taccatgatg ccccttggac cgttcggcat catggatcag gtcggtttaa
     9121 aaacggtttg gaccatcacg gattactggg cggataaaat gaaagatccc caatccaagg
     9181 ccaatgcaaa tttcttaaaa tcttatgtcg aaagaggaga actgggcgcc aaaacgaaaa
     9241 aaggttttta ttcctatccc agaccggcgt acagtcatcc ggaatttctg acgggacaga
     9301 tcaaaagttg atatcggtac gggagggttg atatggatcc gtttcaagac gttcatcatc
     9361 ccgatattga atccgtcctt caggtttcac ggtcaatatt tcattgactc tctttcttgt
     9421 tttttttata atcatctcag ggccgtttta agaggtcaat acaacgagga ggaagcaaaa
     9481 tggcagaaga cgatgatgtt ttgacgacgg atgataatga aagtcctgtt agcggttacc
     9541 tttccattgt attgacaatc attggattgg tgatgctttt gcttattaaa atactgggca
     9601 ttacgtcctc cgatgagacc ggctggtcca ttgaatttgt attgtatctt tttatcagta
     9661 tgacttttgc tattgccggc ggattcctcg gttttctggg attatcagaa gacaacaata
     9721 aaaaaatatt tccgattatc ggtttgattg tcgcggcaat atacgtcgtt tcgctcgggt
     9781 atgttatagc cacgtctatt tcgtaaatgc ttctgataac aataacgttt ctaatccgct
     9841 gctgcttgaa tttttattgc aggtcgcagc ggattctttt ctgcagacaa tcccctgatc
     9901 tttcataaat atcctgtgcc ggttatcgag gcaacaggca tgtttcccgg ccggccatac
     9961 ggaggcggaa aatctgataa acggggattg atctttaatc caaccgtcgc gagaccgtct
    10021 agcggtttgt gatttccacc accaaaacgg tattgtcctc ggcatcggct gccggaatat
    10081 gtttttcctc cacccggccg atggtcgata attgcctcaa caagtctttt atgttgtgac
    10141 ggttgagttc acccgtcaag aaggctttat cccgcatgat tgtcgcggtg atattttttg
    10201 cttcgcattg tctaaatatt tgttctgctt cccgcacggc aacttcaaga tccgtaacct
    10261 tcaatacaat gccgggtttt atatgtgaat cttgcgcccg tggcgcgacg cttgccttca
    10321 gggctgtccc cgaaacgtga cggtcttttg tctgtgcaga ttccacactg aattgttccg
    10381 gcacctgtgc gggagcgatt ttatttgaat catcccgccc gtcggaagcc ttcagttcat
    10441 tgacatccgt ttcatggaca tccgtcaccc ggtgcacttc ttttttacgg acagattccc
    10501 gtggtttgtc tttctggctg atagtgtttg gttccggctg tgttcgttca gcgagggatg
    10561 tgttttcttt ggcttccggc gactgatctt gctgagtctg aagcaccggc gccggtacgg
    10621 atgaaggaac aacttcctga aaacggtttt cccccgcccg gtagaggtaa acggcaagca
    10681 cggcaatgca gaccgtggca aatatctgaa cgggaatttt tatctgcagg ggataaaata
    10741 atttataaag aaaatttttc tttccggctt cctcgcgcac ccgggccatg atttttttcc
    10801 gaaaccaggg cggcggttcg acctcatgaa ggctgtttgt cagttctttc gttttgcgca
    10861 gccgggcaag tgcctcctcg cattgccgac acgatcgcag atgttcgtcc acggctgctt
    10921 tatcttcttt ggaaagcaca tcgtccagat acaaagacag gttgttctca atatccttgc
    10981 attttttcat aagtcaccaa taaccttttg caggcaatct ttcagcgcgt tgcgggccct
    11041 tgaaagccgt gatttaaccg ttccatcagg caccttgagg atggcgctta tttcttcata
    11101 agaaaagccc tgaatgtctc tcaatatcag cacgtcccgg tgttccgcat ccagcgtgtt
    11161 gatgcatttc tggacatacg cttctctttc gcgtttttcc atctgtacgc cgggattact
    11221 ctcctgactc atcccctgat cggagagttg atcggcgttt cttcccccgg tggcgtcaat
    11281 ggaaaatcct tcgcgccggc ttcggccgct caaccgcttt aagcggtttt tggaataatt
    11341 gacaacgatt cgatagagcc aggtggaaaa ttttgcttcc gccttgaact gccggatgga
    11401 tttgaaggcc gcgacaaagg cttcctgggt tacatcacag gcttcatcgt aatcgccgat
    11461 catccggtaa gcgatgttga gcattttctt ctgatgcctt tccaccagaa cgtcaaaaga
    11521 ctctgtatcg cctttttgac aaagcgtgac atatttcaga tcttcatctt tggcggtctt
    11581 tgccctggtg tttattatat tagacatcca tagcaatgct tttgttcccc ttctatgtgg
    11641 ctcatacaga gctcatttta ccgtgatctt gcgtgggaag ccggttgtgt gcattcggat
    11701 acgagaggcg gatcagtaaa aaatcgcttt ggattcgaat tccctcaatt ccctgaacgt
    11761 gttttcacag tcctgctttc tctgttccag acgcgactgc ctggcgacaa tttccgccgg
    11821 atgaagttgt ttgccggcga cattctggtt ccagtcctgg atcatttgat gggcatcccg
    11881 gcagacgtca ttcatttttc tccggagcag ttcaaatcgt ttaatctgag ccggcgaatc
    11941 gaattcaatg cccttgaagt aagaagcata ttccttcatg cccggtttga tgacggttcc
    12001 ggatgtggtt tcaatggcga ctttttccat cggaccgacg gtggccacca cccaggcgtg
    12061 attgcttccc attgccaact gccggaaatt ccacgcggtg atactttcgt gcagggttcc
    12121 gcccattatc tttgcttcaa tgccgttggt caccagttga ttccagacat cgatagccat
    12181 gtccagacaa acgaattcgc cctccagggt atacgtgtgc cccttgtgaa attccgcagc
    12241 aatttgttcc accattttca ttttgtccgg tttgggtgac gcctgccggg ttggcagggg
    12301 cggtatggcc tccggaggag gtggcgcatc ggacggtgta aaaacctgca cgggtggaag
    12361 agctgcggga gaatccgctg ccgccggtgc ggcgggaaca ggtttgggag tcgcctgacg
    12421 gggaggtttt gtaaaatgat aaaaaatcag tgcggccaat atgccgatcg ccatcagaat
    12481 tattacataa tatttgccac ggagaacccg tgatgaatta ttttttggag ccggtgtagc
    12541 cggtattttt tttccgcaga ccgggcattt gaaattaccc ccgtgcagcg tatgcgcaat
    12601 gtcttcggac ttgaattcga aatggcaatg cgggcattca atctgcatga tgcttttcat
    12661 ttccttgtta cgcgttatcc gacaatgtaa gtgctggtgc cgacggcaat cagctcaccg
    12721 ctgtcattga caaattccgt acgcgcgaca gcaaccttgt gtcccagtct taaaagatgg
    12781 ccggtcgcca ggaagtatga tcctttcccc ggacgcatat aatcgatccg catatcaatg
    12841 gttcccatgc cggtcagacg cttgacgatt ttttccaaag gcgcatcctt catttcgtta
    12901 atgatgtgta tctgcgcgat cacgctgccg gtaaaatcaa gaatggtcga tatgacgccg
    12961 ccgtgtaaaa tgtttttctc aaaattgccg atcagatcat cccgcatgtc gatccggacg
    13021 accgctttgt cggccttaat ggaatcgatc ttcagaccga ggtatttatt aaacggcatc
    13081 ttttcttcat agacgatttt caactgctca agaatgacat ctatcggcac agcatccatg
    13141 gtcatggtta tcctgaaaat tttaatgtag agccgccttt tatcacagcc aggctttttg
    13201 taaaacagtt ttgttatcca tcagcagttt catgtaatcg cgttcaaggc ggatcatgtt
    13261 cctgtccttg cgacgcagcc actcggcaag ccaggcgaaa cgcagcgcga tgataaactc
    13321 ggtcagatat cgccagctga tttgggacat gatgcctgac cttttcattc tgtcgataaa
    13381 atgtccgacc aggccgccgg caaggcttcg cgggttttcc atgccgatgc agccgatgag
    13441 attggcaaca tcgtagagtt cgcttttcaa cccgcagaat tcccagtcga tgacgcatcg
    13501 gataccgtcg gttgaccaga taatattcag aggatgatag tcgccgtgac aaaaggccac
    13561 cggcagttct tcataaacgg gcagaaaatc cttttccaga aaatcgacca cgctgccgat
    13621 gtcgtttttg atgtctttgt tgtaaagatt gatttcacgg accaatttgt aaatgtaatt
    13681 cctcagggag aacacgccgc gggagccggc cagtgtcatc ttttgcgatt tgcgacgcag
    13741 ggcgatcaga aagtcagcca gaacaatgcc gcgccacggc tcaaaaacat atttttcacg
    13801 gtccagttcc acgccgggga caaaaggcga aagttgccag aaattattct ggtgttcgag
    13861 aagataattg cctctctcat cggttagata gggattgatt tgagggagtt tcttcgcggc
    13921 cagcaaatcg agaattgccg caatctgttt tcttccagcc gccgattgcg atgcaatttg
    13981 ttccaggacg aagaatcttt tgtctgtatc ttccagaacc acacgaaacg cggaacgttc
    14041 cggacttccc tgaacaggaa ttttacgatg tatttttttc aggggaacct gccacaggcc
    14101 ggccacggct ttcaatgttt ctttgccgaa aagggagtgc tctcctgcgg caacaacgcg
    14161 tctcttcgat gttgttgccg ctgtggacga catcctcggc gcatgttttt tcagggcgga
    14221 cacttttttg tctttgggct gtcttttgat ttttatttcc agtcgcatcg ccgcgcttct
    14281 ttccatcgcc gtaccggtga ccagtaattc caccggccgg cgggagcggg tatatttggc
    14341 ggctttgccc tgatgatgag cggccagacg tttttcaatg ttattggtga tgcccgtata
    14401 caggcttccg tcgcaacagc gcagaatata aacaacccag gttttgctgt ttttcttaac
    14461 gttgatactc atgacctgta ttttctacaa cagcgggaag gacattgcca tagaaattta
    14521 caggcgtatt tccgaatatt cgcttgttta tgaggattaa aagataatcc ttgctattta
    14581 tgaataatac ctgtacaagg cctcacagtg atcgcgatag ttattgacct cccttccttt
    14641 ctacttcaga aatttcggca ggaaaatccg gctaatcgta acagaatcca actttagcgc
    14701 tagatttgca gcctgttttg ggcttgtcat catggcgcac ggcgatgtgt ttttaaacaa
    14761 ccgcgacgaa tagacctggg ttcaaatgtc acaaatactg aaaaaagaag gaaaaagaaa
    14821 aaatgagttt tgagaagttc gagttagatg cgaaaatact ggccggcgtg caggcgtgcg
    14881 gctacaatgt gccgacaccc atccagttac aatctatccc gccgattatg gaaggccgcg
    14941 atgtgatggg cctggcccag accggtacgg gaaaaacagc ggcttttgtg ctgccgattt
    15001 tgcagcggct gatgcagggg gcacgcggtc atgtccgcgc gctgattatt gccccgactc
    15061 gcgagttgag cgagcaaacc catacggcca ttatgcaact gggacgaaaa accaaattgc
    15121 gtagtgtctc catttacggc ggcgtgaata tcaacggcca gatcaacaaa ctgcggaata
    15181 atgtggaaat catctccgcc tgtcccggtc gtctgctgga ccacatcaac cgcggcaccg
    15241 tggatttatc ccggatagaa gtgctggttc tggatgaagc cgaccatatg tttgatatgg
    15301 gcttcctgcc ggatattaga aaaatcgtca aacatcttcc cgcgcagcgt cagaccttgc
    15361 tgttttccgc aaccatgccg gacgacatcc ggtcgctggc caacgatctc ttgatcaatc
    15421 cggtaaatat taaaatcggc cacacggcgc cggtcgaaac cgtgtcgcat aaattttatc
    15481 cggtgcagat gagctctaaa acggatctgc tgaaagatat tcttgataaa acagatatgg
    15541 attcggttct gatttttacg cgaaccaaat cgcgcgcgaa aagcgtggcc ctgcaactgg
    15601 gaaaatcggg atacaaagtg acgtcgctgc acggcaattt gacccagcag aaacgtcagc
    15661 atgcgctcga cggcttccgc gacggccgtt atgaaatcat ggtcgccacc gacattgccg
    15721 cccgcggcat tgatgtgaac agcatttccc atgtgatcaa ttatgatatg ccctcaacga
    15781 ccgatgccta cacacatcgt atcggccgga cgggacgcgc tgccaaaacc ggcgatgcct
    15841 ttacctttgt aaccggagag gatcgcggcc tggcccaatc gctggaaagg gtgcttggca
    15901 aaaagctgca ctaccagaat gttgacggat taacaatgag cttgcccgtt gacgatagag
    15961 tttccgccag ccgtaatagc aggggaagga acatgaagcc tcataacgat aggaagaagt
    16021 tttctcccaa agcgcgggcc aacagcagat tgaatcggcg ttctttcgat tcttccaacc
    16081 gcagtcagtg ggcgcattaa ttcaatcaaa aaacaccatc gggtgatcac tctttaaaat
    16141 atttatttta gaagttatca cccgatggtg aaagactgtt aattttcgtt ctctatcgtc
    16201 gggctttccc gctgatttcc cgtatgcgca cacgaataat cagagttttt ttcattacct
    16261 tttccgaaaa atctccggca tcacttccat actgacgcat aatgcattcc agcgcggctt
    16321 ttttgccctt cacgtcgttt atgatttctg ccgtgcctga accaatcaca ctttcgtatt
    16381 ttgcactcca tgcgcagggc tgtgctgcat caaggatttc atgaaggata tcaaattcga
    16441 atccgacgcg attatttctt ttcaggatat cgatttttct tccttcgggt gcggcatgaa
    16501 aatagaggca aaagccgtca tagccgaagt tgaggggaat aatataaggt tgatcctcgt
    16561 cggacatggc cagacggcac acccgacaac gacggatgat cccgtcaata tctttcctgt
    16621 cctttattgc tctttctttt tttcgcattt tcaatctgac ctgatcgggg ccgatgatga
    16681 tttgtccggc aattatttgt agccggtggc tttaaaaaac gtatagcaga agaggccgtc
    16741 cggttcggca gtgcgatata aatcgcggat accctgatcg aaaacgtttt tcccgatgat
    16801 ccccgcgtcg attgcctctg cacgcacgcc ctcaatcatg gcggtgaacg tttttttgat
    16861 gaatccgtcc actagttgcg gcttgccgga atccacataa accatgcggg gagatacgca
    16921 aacagatcgg aaacccgcct gattcagcaa cggatgcaat gctctgccga tgttggcgtt
    16981 acctccggcg cgccgctgca gttccacctg actctcaatg gccttgtgcg ctgccgcgct
    17041 gtccggataa aaatacgccg agccgtggtc tccttcaata acggtgaccg taccgttttt
    17101 ctttaagtac cttttaagga tgatcaacgc ttcaacgggc tgcggcagat gttcaagaac
    17161 aaaacagaga aagatgtgat caaacgattg cgatgcaaag gataaattaa aaatatcggc
    17221 ctgttcgaaa tgaacatttt gaatgccggc cgcttcggtt tttcttctgg cttcatcaat
    17281 ggaggccgcg gaaatgtcga tcgaagtgat caacgcgccg ggactgtttt tcgccagggt
    17341 gatggtctgt gcgccgacgc cgcaaccggc ttccagcacc gtactgcccg gcgcataagc
    17401 ggtgtcgaca tgcagcaatt ctaccagagt ggaggcttga tcctgaagtc ttatgttctc
    17461 taccggatca taaccgtgaa tgtatgattt ttttatgctc gcttttacag gataaaaaag
    17521 atgtttcgtc atgtccgccc ggttaatcat cctttacagg cgagcggcat taaaggtgtc
    17581 gcaggcgccg atattgccct gttcgaagcc gcgtttaaac caggtgacgc gctgcgcgga
    17641 actgccgtgc gtaaagccgt ccggcaccac gtaaccctgc gtctgtttct ggatgcggtc
    17701 atccccgacg gcgctggcgg cattcaatcc ttcttcaata tcgccggctt ccagaatatt
    17761 tcttttcttg tcggcctgat gcgcccacat gcccgcgagg caatccgcct gcaactccag
    17821 cttaacgttt aatttattcg cttcggattt gcttgtgcgc gactggagct catgcacttt
    17881 ctggataatg cccaactggg tctgaacatg atggcctatt tcatgggcga tgacgtacgc
    17941 ctgggcgaaa tcaccggatg cgccgaaacg tgaatgcagc tcttcgaaaa atgccaggtc
    18001 gaggtaaacc ttgtgatcgg ccggacaata aaaaggtcct gtggccgatc cggccatgcc
    18061 gcatgcggat tcaacggcct cggaaaacaa tacaagtttc ggttcttcat aggtgcgtcc
    18121 cgcctgctga aaaacatcgt tccagacatc ctccgtttcc gccagcacca ccgcgacaaa
    18181 attgcgcgca ttgtcattgg cggctgtggt tgcgctttgt tcaacagaaa ttgaagaaga
    18241 ctgcgtattc tgctcaacga cctgcaatat tgaactcgga tcaacgccca tgaacatgct
    18301 gatgaccacg atggccacca gaccgagtcc gccgatgccg ccgacctttg cgccgcggga
    18361 aatcgacatt cctcggcggt cttccacgtt gtcactttgt ctttcgcccc tccagcgcat
    18421 tgttttctcc tttttagggt tacatcagga ataatatgat tacggcccgg acggccggaa
    18481 tcgaccctta tgcttcagtt gtcagtatag aaacaaggtt ttcggctgtc aagataattc
    18541 atcttcgttg attttattgg agaaaatagt catcaatcaa tccgatgcgg acatgattat
    18601 gctttaagaa atgtatccgg atttgttcag taagggggtg acgcgcagac cgcggaaata
    18661 cgaccgcgat ctgcacgcag cctttgttct aatgcagtct ttcttcggac aaaaacaggt
    18721 gatctgtttc gatttccacc agcgcttcaa aaagctgccg gatccgcgga tgatccgctt
    18781 cggcggctga ctttctgtag aattcgatag cccgtgtttc ccttgcgtgg gactctttca
    18841 gatttccaac attctccgta tggcaggtct cgtttcccgg aggaagggat gccagtttga
    18901 gaatcttttt ccaaatggag gcatgttccg cttcaatttt gcccagcgcc ttgaacaaaa
    18961 cctttccttc gggatcatcc gttttgcctg cggcgcatga ataaaatgtc gcgttgctaa
    19021 tttccacctt gagcgcatgt tcggcattgg cccgatcttt tgcgctcaga ggcacatcaa
    19081 aattcacgac cgcatctttt gcctccataa tatattccat gtgagcgccg cagaacgggc
    19141 agttcgccgg aggactggcg ccgatatacg cgtcgccgca tattatgcat cggtatagtt
    19201 taaccatgtg ttctcctttt aagataatga tgcagcgtta acttatgctt tccacaagcc
    19261 gtgaagattg cagtattcgc gcgctgtaat gctgattgct tcgatgggga aaaccgcctc
    19321 gggcgcctgg ccgggcgcta aatactggcg gtaaattttt ccgtctgcga taacttcaat
    19381 ccattcaatg tagtgctttt cttccatggg atgcgccacg ctgccaacgg tcaccttgaa
    19441 accgccggcc actttttcca ccacggggac atgtttttcc ttggctgcat ccgtcgtgtt
    19501 ttccgtcatg agtttcatgg gctgatcgca acacacaagc tttcccacgc ccgcgtgaag
    19561 tatttctacg atattgccgc atacgttaca tttgtagact tcgtttctgt tagccatcat
    19621 aaacctcctt gttttattat gattaatcta ctttcttaaa catctttccc ttggcgccgc
    19681 agaccggaca ggtttccggc gcttcgtttt ccacggtata accgcagacc ggacaaacat
    19741 aatagctgta tgtttcctgt gaaccgtcca gatgatccag cgctttctga tagagggcgg
    19801 cgtgaatttt ttccacttcg ttggcaaagt tgaaagaccg ctccgcttct ttatgacctt
    19861 cggctttggc cgcttcaatc atggccggat acatgctttt gaattcgtgg gtttctccgg
    19921 caatggcttc ctgaagattt tcttttgtgg aacggatgcc tttcatcgcc ctcaaatggg
    19981 cgtgggcgtg tacggtctcc gcttccgctg ccgcccggaa taattttgcc gcctgggcaa
    20041 atccttcctg atcggccttg gctgcaaacg ccagatattt ccgattggcc tgtgattctc
    20101 ctgcgaaagc ttcctttaat gcttcttctg ttttggacat tttcatatgc tccttcgttt
    20161 tattttttta tgaatcaatt aaaaatttgt cggtaaaggc atcacactcc gaattcagcg
    20221 acgtctgtat ggctgccgct gaatccgggg aatgatcaat ttacgccagg tcaaaacggt
    20281 ccagattcat gaccttgttc caggccgcca caaagtcgtc caggaacttc tcctgcgaat
    20341 ctttacaggc atagacctcc gccacggccc gaagctgtga gttcgatccg aaaatcagat
    20401 caacgcgggt gccggtccat ttgagctcgc cggtcttgcg gtcgcgccct tcaaatacat
    20461 ccgcatcttt cgacaccttc cactccgtgc ccatgtcgag taaattcacg aagaaatcgt
    20521 tggtgagcgt tcccggacgc gaggtgaaaa ctccatgcgg agaaccgccg aaattggcgc
    20581 ccagcaaacg caggccgccg atcagcacgg tcatttccgg cgccgttagc gtgagaagct
    20641 gagccttgtc caccagcatc tcttcgggca atacggtgta tttggccttc tgatagttgc
    20701 ggaatccgtc ggcttttggt tccagcacgc caaacgaagc tacatcggtc tgcttctgtg
    20761 aagcgtccat gcgacccggt gtgaaaggaa ccgtaatttt gtaaccggca ttcttcgccg
    20821 cctgctcgac accggcgcag ccggccagaa cgatcagatc ggcaagcgat accttcttgt
    20881 cgcctttctg gtctttgttg aacgctttct ggatgcgctc cagcgttttg agcacgccgg
    20941 ccagacgttc cggttcattc acctcccaat tcttctgcgg agccaggcgg atgcgggcgc
    21001 cgttggcgcc gccgcgcatg tcggagccgc ggaacgtgga ggctgacgcc caggccgtcg
    21061 aaacaagttc cgatatggac agacctgaag ctaaaatctt gcttttcaag gaattggcat
    21121 ccgctttgtt aatcagctta tggttgacgg cgggaatggg gtcctgccat atcagcactt
    21181 ctttggggac ctccggaccg agatagcgcg aacgcggacc catgtcgcga tgggtcagct
    21241 tgaaccaggc gcgggcaaaa gcgtcggcaa attctttcgg attggcaagg tatcgccgcg
    21301 caatcggccc ataaatcggg tcgaaacgca gcgagagatc cgccgtggtc atcatcgggc
    21361 ggcgcttctt ggacgggtca tgagcgtctg cgaccatgtc ttcgtcttcg acgttcttgg
    21421 ccagccactg attggcgccg gccgggctct tgaccagttc ccactcgtat ttgaacagca
    21481 ctttcagata gcccatatcc caggtcgtcg gattcggttt ccatgcccct tcaagaccgc
    21541 tggagatcgt atcgccgccc ttgccgcttt tataactgct tccccagcca agaccctgcg
    21601 cttcaagggg agccgcttcc ggttctggtc ccacatgggt cgcaggaccg gcgccgtggc
    21661 acttgccgaa ggtatggccg ccggcaacca gcgcgacggt ttcttcatcg ttcatagcca
    21721 tgcgcgcaaa ggtttcgcgc acgtcacggc cggaggcgat cggatcggga ttgccgtccg
    21781 ggccttcggg gttcacatag atcagcccca tctgcacggc cgccagagga ttttcaagat
    21841 cgcgctcgcc cgaatatcgg ctcttgggct tgtcgctggt cgccagccat tcctcttcgc
    21901 tgccccagta gatgtcttct tccggttccc agacgtccac gcgcccgccg gcaaaaccga
    21961 aggttttaaa tcccatcgat tccaaggcgc agttgccggc cagaatcatc aggtcggccc
    22021 aggagatttt tttgccgtat ttctgcttga tcggccacag aagccttcgc gccttgtcga
    22081 ggttcacgtt atcgggccag gaatttagcg gggccagtcg ctgattgcct gttcccgcgc
    22141 cgccgcggcc gtcacccatg cggtaggtgc ctgcactatg ccaggccatc cggatgaaaa
    22201 gtccgccgta atggccccag tcggccggcc accagtcctg cgaatcggtc atcagcgtga
    22261 tgagatcttt cttcacggcc tttaggtcta gtttcttgaa ttctttggcg tagtcaaatg
    22321 cttcgcccat ggggttgctc ttcttgttct gctgatgcag gatcttgagg ttcaattgat
    22381 tcggccacca gtcccggttc gatttgccgc cgccggaaat gggttttttc atcatgcctg
    22441 ttaccgggca tttgctttct tcggtcatct gatttcctcc tttctttcag tgataggggt
    22501 taaaaaacct tattcctttt gccttgttgt tttctcagta atctggaatg attctgaatt
    22561 agaataaaaa ccggtcatcg tcattctttt accgtttgtt taaaataccg cgggactgcg
    22621 caataagaaa attcagtctg agcgcttcag gcaatccctg caatacccgt gaaactccag
    22681 tcgtgtttcg gttacgacaa aacccgattt ccgggcaatg tccctgggtt caagcgaggc
    22741 gggaatggcg tagtcaaaaa tcttcccgca gcggttgcag atgagattca catgatccga
    22801 aaggtccaca tcatagcggt tttccatccg gtcgaattcc aattgtcgga tcagaccgtt
    22861 ttcagaaaac tccttaagcg tcgcataaac ggtggacaaa ctgatacgac gcgctttctt
    22921 actcgctgcc tcataaatca aagtagcgcc ggggtgagct tcgctatgct ccaccagcgc
    22981 atcaataatt gccagacgct gcggggttat cttgagacct ttttccttta attggccgat
    23041 caggccgtct ttatatatca taagtattct ggaatcattc cggtttatgg aaagatacaa
    23101 tcatgcctat aaattgtcaa gacatatttt ttcatggttg accggcggga ttgacaaacg
    23161 aacttatcaa ttatatgaaa caaataataa ttattgagag atttcatatg ccggtttatt
    23221 tacaagtaat cctgctggtt gccatcttta tcgccactgt gatcattttt ctttacattg
    23281 gcggcctggc tgtgcggcgg gccagttttc agattattgc cgaaatggaa gaagccagag
    23341 cctttaaaga gggaagggcg attaagattc aggacgagcg gaagaacttc ttccgcgtgg
    23401 ggaccaaaaa tatccgcccc cgggcgctga atctgctcat cgctgatggt ctggtgatta
    23461 aaaccaacaa cgggaaatat tatctggaca aagaaaaact cgcccaagcc aaagctcaat
    23521 taagttcaaa aacataaaat gaacatttct tcttaagctt atcttttata cttccgttgt
    23581 tcatactgct taatgggctc ttcgagaggt ggaaaaatct cgtgaaatcc cttgtgaaga
    23641 tttgtagcaa tcttcgtgat gaaattggca gtacggtggg cggtttcttt agtttcacgt
    23701 aatgcctgtt ttaagcgcac gctatcgcca ctgtcagtaa tgtacttttc gtcagcttca
    23761 ccacccttgt gctcataaac gtgcctacga tgaaacatta gagtaccaaa agatacatcc
    23821 tcgctatcga tactactgag aacgtctatg tcaaatactt tgcgaaacat ctcggaaact
    23881 gtcccgatgt tgtggaagtg ggatttctct atccaagctt tacgggctgg agttaatggc
    23941 actctggtta ggagctgctt tgcgtagtgg ccggcgaacg agtcgaaaat agcgacggtt
    24001 tctttaacac acgtttcata tggaccacca gcattgattc tatctctgat ttgctgaata
    24061 tttttttcca actcctgcaa atcatttctt gttccacatg agctacagta agcatacgtt
    24121 ccaaggacgt cattaacttt accacaggca ttgcatgtaa ataggttttg ctgacgttct
    24181 tctgcatagt agaaagctgg cttctcgcag tctttaccag ccgcttcagc caccgaatcc
    24241 atatcgatta tgtgttcgcc agactgacca gaagcaagag cgttactaag tacttcacag
    24301 tattgcctaa catatcgttg ttgggcatca gtaaggaaat ggtagtactc atcggccgta
    24361 aaggcacaat acggacagat cttgccacca agacctgctc gccaataacc attacatgag
    24421 gggcattggt ggccaaacat gcctgtgctg tctgatgaga taaatactgg aaaacagtcc
    24481 ggcaacggag gtggattcca aggagtgccg attccaccca tcttaatgtc atgaacggca
    24541 acgccttgcg gaatggcata aacagcaaac aatgccgcag gggttggtct tgaatgagtc
    24601 aatccaacat tgtaggcgat ccggccatca gcgtcgatct tgatgttaaa attaaccttt
    24661 cctcctgtat gtgtaatttc ctcaagttct gtctttctat tcaatatttc cccccacggt
    24721 ttggtgtaaa tgtgatcaaa ctttctcact ccgtctttgt cggcacacaa caattaatat
    24781 gcgtacttca tttattttga tattgtggat gctaaaaaat aacacaactt tttatttttc
    24841 aaaagaaaaa atcctatgtt ttagggctgt tcaaaaatgc tcagatgcaa ggcgtgcaag
    24901 aatcgtatcg cgaggcgtat atgaacatac gttgagcggt gcagtttgca gcacaacgcc
    24961 gcagatggac gttttttagc ggccctggag ttcgtgtttt ttcatcataa tcaacactgc
    25021 cgaaatcgcg ccttcggtca tgcccggaat gcgctccatc tggccaagcg tctgcggggc
    25081 gattttggtg agctttaatt ttaattcgtt ggaaaggccg ggcacggagt tgtagtcaaa
    25141 gtcaggcggg attttctttt tttcctgatc ttttaatttc ttaacggcgt cgaactgccg
    25201 cttgatgtag ccttcgtatt tggtttcgat ttcaatctgc tgtttgacaa aagcatccag
    25261 ttccggttcc cagccgggaa aaatggaaag gttgtcgtat gacaattcat gtcgtttcaa
    25321 aaggccgtgg agcgttgtgt gattgctcaa cggcggagaa ttgagtgttg cgagtttttc
    25381 gtttgtttcc gcagtcggtt tgaccggcga tgccttgagc tgctcaatac cattctctat
    25441 tttttttatt ttatcctgaa gttccagata atgcgttttg tcaattaacc ccagttcgca
    25501 acccttgccc atcaggcgaa tggttgcatt gtcttcgcgt aaaatcaggc ggtattcggc
    25561 gcgtgaggta aacatgcggt aaggttcatc cacgccgcgg gtaacaagat catcgatcat
    25621 caccgccatg taggcctcgg aacgatccaa tagaaaagca ggggctccct gaacggcgca
    25681 ggcagcgttg attcccgccc agagaccctg tgccgcggcc tcttcataac ccgatgtgcc
    25741 gttgatctga ccggccagat acaggccatc aactagtttt gtttccagcg tgggtttgag
    25801 ctgtgtcggc tgaacaaaat cgtattcgat ggcgtaagcc gggcgcatga tttgcgcctg
    25861 ttccagtcct ttgacgctgc gcacaatctt atactgcaat tccagtggca gacagttgcc
    25921 cagacctttg gcgtaaactt cctgcgtgtc gagtccttca tgttccaaca ccaccggatg
    25981 actctggcgt tcggcaaagc gcatgacctt gtcttccagt gaggggcagt agcgggcgct
    26041 cacgcctttg atgatgccgg agtataaagg agaatgctga atgttttcgc gtatcacgcg
    26101 atgcgtttca gcggatgtgg cggaaaaata cgacggtagc cgctcggtgc gcagtttttc
    26161 ggttgtgaag gaaaagggcc ggggatcggg atcgctgtcc tgccgttcca agcgggaaaa
    26221 atcaatggaa gaggcgcgaa gcctgggcgg cgttcccgtt ttcatgcggc cgatatcgaa
    26281 gccaagctct ttaagatggt tggccaatcc gatggacgcc agttcaccgg cgcgtcccga
    26341 tggagtttgc gaagggccga cgtgcaccag cccgtttaag aaggtgccgg ttgtaataat
    26401 aattttttta gctccgtaaa aatgctcgct ctgatccagc acgcctatga cttttccgtc
    26461 ttcaatcacc agtttttcaa tcatggcctg ccggacataa aggttaggtt ggttttcgac
    26521 cacggccttc atcgtcagac ggtagagttg cttgtcgcac tgcatgcggg aggaatgcac
    26581 cgccggacct ttggaggcat tgagcaggcg aaaatgcacg gcggttttat cggtgatctt
    26641 gcccatctcg ccgccgaggg cgtcaatttc cttgaccagt tgacctttgg ccagtccccc
    26701 gatggccgga ttgcaggaca tcaacgcgat ggaatccaga ttgatgttga acagaatcgt
    26761 ccggcaaccc atgcgcgcgc aggccagcgc cgcttcgcag ccggcatgtc cgccgccgac
    26821 aatcgcaaca tcataattgt ctgttaagtt cgtcattctt tcaatcaaac acttgctctt
    26881 gttttaaatc aatagtaaac acgaacaggt catgccgttg caaaacggta tccagtattt
    26941 tcattaagtt ctggataccg gccttcgccg gtatgacgcc attgatggta ctttctattc
    27001 atgacaagtt ttttacggca tagaatgtaa aaaaacaaca taaaaaagag gttgctttgc
    27061 ccaaaccgga tttttggaag aataacaaaa actactacga cctgaaaagt tactggcgca
    27121 atctgttcgg ctgcagcgtg cacaagctgc aaattgacgc aggatttacc tgtccgaacc
    27181 gtgacggtca tgtggccatg ggcggctgta tctattgtga cggcagaggt tcaaagttgc
    27241 gtcaggctgg tgagttgccg tcggttaccg agcagattgc gagcggaaaa aaatattata
    27301 caaaacaggc cggaaagttc atcgcttatt ttcagacatt tacgaatact tatgctccgg
    27361 tggagaaact gcgcgcgctc tatgatgaag cgctcaatca ggaagacgtt atcgggcttt
    27421 ccatcggcac caggccggat tgcctgggtt ctgatgttgt cgagttgttg agcggctacg
    27481 cgcaaaagta tcatgtctgg gtcgagctgg ggctgcaatc tgttcatgat aaaacattaa
    27541 cgttgatcaa ccgcggtcac agttttcagc aatttgagga tgctgtaaat gctctggccg
    27601 gacacggcct gaatatttgt gtgcacatca tcatcggtct gccgggcgaa agcgacgaag
    27661 atgtgctggc cacggccaaa acgcttgcag ccatgccgat tcatggcatc aaaatccatt
    27721 ccctgctggc cctggacgga acggctcttg gtgaaatgta caagaaagga cgcatcaaca
    27781 tgatcaccaa agaaaaatat gtttctctgg tggccgatgt tctggaggtg ctgccgccgc
    27841 aaaccgtgat tcagcgcctc accgcggatg gttaccgcga tattttcctt gcacccgact
    27901 gggcaatgaa caaactggac gtgttaaact caatcgataa agaactgata cggcgtgatt
    27961 cttatcaggg gaaaaagtac cggacaaatc gcgctgaatc acttgattga tatttcctta
    28021 gttcgaaccg cttcaaccct tcaataagcg ctatggctta tctttttttg accgtttgga
    28081 attcagataa aatccataac cgataaaaat caaaataaat actaacgacg gggggaagaa
    28141 ggcgacaatc aagatgcaga cgaaagcgat gacaagcatt cctgaatacg ggtgatcttc
    28201 caatgcatcc aacatcggat taaacaatcg atctatcatt cgctcttttt cttccattgt
    28261 tccgaaatcc ttttgtttat gcatggttgt ttgtgcaatg tcttattgac atcatagcat
    28321 aattcttgat cttttaaaga ggcaaatatt ccaaaacgac aggaaggagt gtccgttggt
    28381 gaggcttgaa atgaaaataa aagtccatgc cgctaattta tcaaacggaa caaactgatg
    28441 atcaatgaaa atatcagtaa aattaagtat agaatggtaa atatctcggc ggccctgaag
    28501 tctttgattt tcttcgccat taaatcattt aactttccgt ccttgtagat ccaccagaga
    28561 aatgtcacag aaaaaattat tataataatc cacaaaattg cattgttcat ttcattattc
    28621 ttccatgtac ggttttacat gagcatccca ttcggcttca gatttgcacg tcttttctaa
    28681 cacattacac gtaatcgttt tgccgttttt ttgggaacgg accgccccgt atattttatt
    28741 tcccttcatc tctctcaaaa cctttaaggg tttttttgta tagtttaata aaatgaaaca
    28801 tttatccagt ctcgtgttgt aatggctctg atgggtaaat ggatctctga aaaatttttt
    28861 ttcgtcaacc ctgacccatg cgtcacaatc tttttcgcat ttcatcttta atgcatggag
    28921 ttgctcctgt cgctcggacg ttacgcaaat gtcatggtat atttcttccg gcttgcttcc
    28981 ggggatgatg cccgtccatt cgctattatt atatgtttct tcatataaga cgctttcatt
    29041 gtcgtcataa tttaccagct tttctatttt actctgtttg tttctgcaat ccatttttaa
    29101 caaaatcgtc tggtgatcga gtttctggta tttgattgat ttcttgggat ccgattctct
    29161 gaaatgttca atcaaatatt ttctttcatc gtcggcaatg gttctgtaag tccatacaga
    29221 tatgctgtcg gccgattgaa ttaaatttat tttattatag taaaaatcat cgcttaaatg
    29281 tttccagact ttcgggtcgg gctgctccga aaaacaaacg ggaggtgatg acacaatgga
    29341 cgctgcgatg aaaacaacga gagatgataa aaacattttt ttcatcatag gaatctccga
    29401 acaattcata gccgacttat ttcataacaa gttgaaagta tcataacact gttccttaat
    29461 ttaaaaaaag agaaatatca accgcgttaa ctggatctgg aaatcttgca cggtggctga
    29521 taggaacgat tttggaatat cgggtgataa aaaacaagag agaaaggatg aatgaccgga
    29581 aaacagcgcg gcagcggtat tatgggtctt ctgtatacat ggcttcgatg atatctttgt
    29641 attgcgcaga gatggatttt cgttttactt tcatggtcgg agtaacttca ccgtcttccg
    29701 tgtagagttt cttgtcgagc aaatggaatt tgcggatgtt ttccacgcgc gcgatttgcc
    29761 tgttggcctt ttccacctcg tcggaaataa gtctgatcac ttcctccgca cgggttaggc
    29821 tcgcaaacgt tgtgtattgg atcttatggt cctgtgcgta tttgacgacg ttttcttcat
    29881 caatgacaat taaagcggtg atgtacttgc gccgatcgcc gataaccacc gcgtctgtaa
    29941 tatagggcga gaatttcaac agattttcga tatactgcgg cgcgatattt ttgccgcctg
    30001 cggtgataat caaatctttc ttacgatcgg tgattttgag ataaccttcc gcatcgattt
    30061 cgccgacgtc gccggtatga aaccagccgc cctccagcgc ttccgccgtg gcctccgctt
    30121 ccttgaagta tccctgaaaa attccaccgt gtttgatcag gatttcaccg tcttcggcaa
    30181 ttttcacttc ggaatccggc agtgcgcgac ccacggttcc cactttgaaa tgttcctcgt
    30241 cggacatgtg cgtaatgccg gtcgtttccg tcagcccgta accttcacgg atcggaatgc
    30301 cgatgctctg gaagaatttc agtacctcat gggagatggg ggccgctccg gaaaatccca
    30361 gccgcacctt gtcaaatccc aggcgttgtt taagtttcca gaaaacgctg aaatacgcca
    30421 attgataacc aaggaataaa taccagggga tcggtttccc ggccagtttg aaaccgttgt
    30481 atgttgttcc gatcttaaag gccagattaa aaacagtgcg ttttaaccac gtggcatcgg
    30541 ccattttcag gacaataccg gaatgatact tttcccagat gcgcgggacc gcatgaaaga
    30601 gggtcggaga aacatcgcgc aggtcgttca tgaccgtgtc ggtgttttcg gtaaaattga
    30661 cgatatgccc cgttgtgaga tgcatcatca agtcgaatat ctgttcatag acgtgattga
    30721 gcggcagaaa ggaaatggtt tcatcatcga cgctcagttt ggtaattttc tggctttgac
    30781 gcgcaatcca taaataattt tgatgggaga tttgcgcgcc cttgggcgga ccggttgtcc
    30841 ccgatgtata aatgatggaa gcggtatctt cgctctctcg tccggcaaca atcttttcat
    30901 acagacccgg tctttcctta tcgatctttt ccccaagctc cagaagcttt tcaaaactca
    30961 tcagcatggg atcgtcaaaa tgcttgaggc cttccatatc ccagacgatt atttttttca
    31021 ggcgcggtgt tctttcgcga aatgccagag ccttatcgaa ttgttcttca tcttcgcaga
    31081 aaaaaaccac cgattcggaa tgcccgacaa catattcgca ttccgccgta ggattggtcg
    31141 tgtacacgcc gacaaagata ccgcccagcg acatgacccc catggccgaa aaaagccact
    31201 cgggtttatt ctcgccgaca atggccacgt gatcgaaggg cttgacgccc agcgcgtaca
    31261 atcccaccgc cactttcttc acgttttcat aatactgtct ccagctaatg tcgtgccaga
    31321 tgccgtaatc cttcatgcgc atggcaaccc tgtccggctg tttgtcgacc agccatttca
    31381 gcccctgagg aagagttttg atgtcgtttt cagtcatatg tatacctcag cggaaacgca
    31441 ctttgcggcg ataacgtttg aagcctttga ccgatgtttc cgtggcaatg cccagataaa
    31501 attctttgac gtcgtcgtct tctcttaaaa tttccggtct gtccgcacgg acaatgcgcc
    31561 cgttttccat gagaaaagcg taatgcgaat atttgagtgc catgtttacg ttttgttcca
    31621 caagaaagat cgttacgccc ttctgattga tgttttgaat gatactgaaa atctctttgg
    31681 tcaataaggg cgaaagtccc agcgacggtt cgtccaacat gagcagtttc gggcggctca
    31741 tcagcgcccg tccgatagcc agcatctgct gttcgccgcc gctcaaatgt ccggcctgct
    31801 gatttttgcg ctcgcttaat atgggaaact gtttgagaat cccgtcaatg tcatcctgga
    31861 tgccttttct gtctttgcgc gtataggcgc ccatgagaat gttttccatc acagtgagct
    31921 cgggaaagac ctcgcgtcct tccggcacat agctgatacc cagccggaca atttcctccg
    31981 tgtccattcg gtcgatcctt ttacccagaa attcaataga tcctttttcc ggctgatcgt
    32041 catccaaaag gtgcatgatg gttttgaggg tggtggtttt gcccgctccg ttactgccga
    32101 gaagcgccac aatggagcct tctttgattt cgaacgaaat gccgtgcagt gcccgaatca
    32161 agtcgtacca ggtttcgata tttttaaccg agagcattat tcctcctctc ccagatacgc
    32221 ggcaatgact tcggggttct tttgcacgtc ctgaggcgtt ccctcgatga ttttcacgcc
    32281 ctggttgatc gccagaatcc tgtcggaaat gcccatcacc atattcatgt catgctcgat
    32341 cagcagaatg gtgactttgt aatcttcctg aatatccttg atccagatgc gcagatcgtc
    32401 tttttcttcc gtgttcatgc cggcggacgg ttcgtccagc agcagaactt tcggttccaa
    32461 cgccagcgct cgtccgagtt ccacgagttt gcgtttgccg tagggaaggc tggaaacaaa
    32521 aagatctcgc agggactgga gatcaagaaa gtcgataatg tgttccacga tttcccggtg
    32581 tttgagctct tctttcgcgg cgaaagaacg tttcccccac atgaaagcgc cactcgtgat
    32641 tcctgtcttc atatggatgt ggcgtcccaa catcaggtta tccatgacag tggcgttacc
    32701 gaaaagctgt atgttctgga acgtgcgcgc cacacctttg cgggcaatgg tgtccggacg
    32761 caggccgaca aggttttcat tatccagaaa aacttccccg gcatgaggtt tgtaaatgcc
    32821 gctgatgata ttgaaaatcg ttgtttttcc gcttccgttg ggaccgatga tcgaaaatat
    32881 cttattgtac tcaacctcaa agctgacatc gctgacagcc cggatgccgc cgaaggagat
    32941 gttcaggttt tccactttaa aatgcgccat acactgcgag cctgctgatc gttattggca
    33001 acgaaaatat ttggtgggag gcagggcatc ccccgcctcc ggcgatattg gtatgttact
    33061 ttttcttcca cgttgccagt tcgttagatg tccagtcctg aagaataata tagctggaat
    33121 tcggaccgca tttcagaatc tgaatggaat cgcaaccctg atgcactgtc gggctccagt
    33181 ttacaacggc gcccaatccc tggaaattct ttgttttatt gagttctcgc agcagggctt
    33241 ccgtgctcag tttcgggccg acccgcttga gcgcatccac caacggttcc gcaaacataa
    33301 tgccggcggt gtagaaaacg ccgaaacgct cttcaggtgc gtatttcttg gcagccgcgc
    33361 gatatttatt catcagcgga tgatccacat cggtcggcag gccgattccg ccggtgatga
    33421 cgccttccca caacccaccg gtaatcttgt tcatcagcgg aaaatccatc aacgtgtcgg
    33481 aagaaatcca ttgcggttta aatccgatgg ttgcggcagt tttcagcgcg attgccgcag
    33541 aggtgggatt gacccacaga aaagtcactt ccgcgccgga atttttcagt ctcaacatct
    33601 gcgaggaaag atctttttcg ccgggttcaa cggatatctc ttcgacaagt ttcatcttat
    33661 acgtattcaa gcgagccttg cagcccttga ggccgctctt gccatatgcg tcgttctgat
    33721 acaggaatcc gatttttttg gctttcagtt tttccaccgt atatttggtc agaatgctgg
    33781 cctcatcatc atagagagta tacatggcaa agaggtacgg attgacggga aacacatact
    33841 cgtcaatggc tgtcgacgga ttgacccaga tgattttatt ctgcgccaga taatctttca
    33901 cggccattcc gcaggcagca ccgacgccgc ccacaaaagc aaaaattccc tcacgctcga
    33961 caagttcctt gaccacggct ttagtctggg cggggttgta ctgatcatcg cggatgaaat
    34021 atttgatttt acgtccgtgg atgccgcctt catcgttgac aatttgaaac agaaggccgc
    34081 tccctctggc gaccgatccc cagggtgcgg caggtcccgt ctgcggcccc cactgggcga
    34141 tacggatttc cttgttgtca aagcccgatt tggcctgggc aaaaacaaga agcgaagcaa
    34201 taaaaacagc gatgacaatc gtcaatgcaa tgatgaatct tttcatagtg acctccatgc
    34261 tttaatgagt ttagataaat gattaacctc tttatgccgt tatggtctgg gggatcaacc
    34321 cttgtaccat ttcccttttt ctttcaggaa cgtgaagctg taattaaaga tactgttcgg
    34381 acctgcgtgt ccaaacgtca cttccacctc cacctgaacc aatttgtcca cagcgggata
    34441 attgtgccgg ttgtggttat cccgcagatg gtaaattctg acaattttac aggtgttcaa
    34501 tgccgcggaa gaattttcgg cgatgtcttt caaatattct tcgtacgtgt gtgtcttttt
    34561 ataatccgat gaaggcgcca aaagcgcata gacctgccgc gaatcttttt tcatttcggc
    34621 agtcagatag tttcgggcgg tttcttcaat gacgcttttt tccagaaaca attcgaggcg
    34681 gtttcggggc gcatcccaga taaccaatcc gaaaacaacc agcaccgcgc aagccaggat
    34741 aaggattttc acggttttac tcatgataat gctccgatgc ttcacgttac actttcttga
    34801 tataatgttt cgcgaacagt ccgctcggcc tgacggtaat gatcaggatg ataacaacaa
    34861 acgcgacgat cggtttccat tccggccagg cgtaaccgaa gaaattttcg ataataccca
    34921 aaatcaggcc gccgacgacg acaccgggaa tgctggtcag accgcccagg acggccgcgg
    34981 caaaagcgcg cataaacggt tccatcataa aattgggatc cagcacggaa cgcgccgcga
    35041 agaacatggc cgcgacggca ccgatcagcg aactcatcgc ccaggtaatg gagaatactc
    35101 tttccacttt gatgcccatg atcttggctg cgttgacgtt ttgctgggtg gcgcgcatgg
    35161 cgatgcccag cttcgaataa cggaaaaata agaacaggga tatcatgatg atgatggaca
    35221 gggaaaagac accgatcgtc cattcgttaa taaagatgcc gggcagcatt tcatgtgtct
    35281 tggtaacgga cagcagaaag ggaaaatctt tctgttccgc tccccacttc cagccggcca
    35341 gtcccataag cagcatttcc gcgccgagcg taataatcag aagccccagc acggtcggat
    35401 ctttggccgg acgcagaaaa caaaactcga ttgccatgcc cagcaggatg gcaaaaatca
    35461 gcgtaacggg aaaggcgagc cagaagggca cgttatagac cgtcaagaca tgataggcga
    35521 caaaggttgc aaccattccc atttcaccct gggcgaagtt gatgacgtcc gatgttttgt
    35581 aaatgatggc gacggcgacg ccgaaaagac catagcaggc cccgatggcg attccctcga
    35641 tcagcagcat ttcaaacatg acggactccc ttcagctttt ttaaaaaggc caggtcttcc
    35701 agtatttttt gattcggatc cagatgccga acatgcccag cggttcaaaa atgacgatcg
    35761 tgatcatgat gagtcccagt gcaatgctgg agaaattggc cagcccgatc accgtaaacc
    35821 atttttttga taccgtcgtg agaagtgccc ccagataggg cagattttcc acatttttca
    35881 ataagtcgta ctggagatag gtgataagca tggcacccat gatagcgccc ataatcgatc
    35941 ccaggccgcc gacggtaatc atgaccagaa agatgatgga aagaatcagg ttgaacgtaa
    36001 agggatcgaa aaagccgaca acaaaaccgt aaaggccgcc cgccacgcct gcgtaaaaag
    36061 cgcttacggc aaatgccagg gttttataaa tcgtcagatt gacgcccatg acttctgctg
    36121 caatgtcgtc gtcccggatg gacataaacg ctctgcccac cctggtcttc atcagattgc
    36181 gcgcgccgat gaccagcaaa acagtcataa tcaatatcag atagtaaatc tttacatcac
    36241 cctggatgac gtaactgaac gatgtccctg aaatacccat atcaagcggc ggggcggtga
    36301 ttcccatcct gccgccaaag aaatcccacc ggccgatgat gtgcataatg gtcaacccga
    36361 agcccagagt ggcaatcgca agataggggc cttcaagcct caacgccgga aggccgatta
    36421 taaagccgaa aaaggctgca atcaacgcgg ccagaatgat tgcagcgaag aacggaaagt
    36481 ggaaattcat catgattaat gatgtgccat aggcgccgat ggcaaaaaaa ccggcatgtc
    36541 ccagcgatat ctggccggtg tagccgacaa ggatgttaag gccgaccgcc agaatgatgt
    36601 taaccatgat aatatttaag aggtagatgt tataagacgg taaataaacg ggaagggtaa
    36661 aaagaaaaac cagtaatacg atggaccacg tcagaatcgt gccgcttcgg aataactcaa
    36721 cgtcctcata gtaatcgcgt ttcatgtcca tggcataacc acatatcgat ggttggttga
    36781 catgttgctg tacgttgatc ttttttgcgt ctggatgttc taaaaacata gcaagacgtc
    36841 cgaaatgtgt caagataatt tgaccaggaa caaaattgta tcagtgtggg attaatgtat
    36901 aaaatgataa aaattaactg taaattgaac agaaaaattg ctaaaggcat attccagaaa
    36961 tttggtaagg aatgaacaat gatcgcgatt atcgactata acgccggaaa tattaccagc
    37021 gttgcacgcg ctctgcagag tatcgggcag gaattcatca tcacggatga tgcgaaaaag
    37081 ctcgatgcgg cgtcgcgggt gattttcccc ggtgtcggcg cggcgggcga agccatggct
    37141 tatctgcgcc aaaagaaact ggatgtctgg ctgcgagatt acatcaaaac cggcaagcct
    37201 ctgctgggca tttgtctggg gacgcagatt attctggatt actccgagga aaacgacacc
    37261 ccgtgcgtga gtcttgttgc cggttcgacc aggcgtttcc cggaaacatt agcgttcaat
    37321 ggtgagttat taaaaattcc tcatatgggc tggaatagcg tcaggctgct gcgcagccat
    37381 ccggtgtttg atcatgtttc gccggaggct gaattttatt ttgtgcattc ctattatccg
    37441 gcgcctgccg atgaagcggc gattttaggc gtcaccgaat acggcataac gttttgttcg
    37501 gtgcttgcca ggaacaatct tgtggccatg cagtttcacc cggaaaaaag cggtcgcccg
    37561 ggccttcaga ttttaaagaa tttctgcaca tggaggccgt catgatcagc aagagaatca
    37621 ttccctgcct cgacgtgcgc gacggcaagc tcaccaaggg gatcaagttt aaaggcaacg
    37681 tagatatcgg cgatcccgtg gaagcggcgc gggaatatta cgcacagggc gcggatgaaa
    37741 ttgtatttta cgatattacg gcgtcgtcgg acaaacgcaa catcatgatc gatgtggtgc
    37801 ggcgggtggc ggaaacgatt ttcattcctt tctctgtagg aggcggaatc cgcaatctgg
    37861 atgatatgcg gaacgtgatc catgcgggag cggaaaaagt gagcgtgaat tccgccgcgg
    37921 tggaaaatcc gcaaatcatt acgcaggggg caaaagcttt cggcagccag tgtgttgtgc
    37981 tgggcatgga tgtgaaacag gtggacgttt ccgatgccat tccatcgggg tatgaaatcg
    38041 ttgttcacgg cggccggatt tataccggaa ttgacgcgct gtggtgggca acggaagcgg
    38101 agaaacttgg cgccggggaa atctgtctga attccatcga cgccgatggg gtgcaaacgg
    38161 gctatgaatt gaatttaacc cggctgatct ccgaaaacgt gcatattccg gttatcgcgt
    38221 ccggaggcgc gggaaaaccc gaacatctgg cagacgtgct gaccaaaggg aaagctgatg
    38281 cggcgctgat tgcttccatg gtgcattatc gaacctatac catcgctgaa atcaagcact
    38341 atttgagcga aaagcaaatc aagacaagat tgttctggta atttttgctt tttttcacta
    38401 ggaaacagta ttgtgaagta ttataatagt aatagtttta cgctaggagg cgatataaag
    38461 tggataaaaa gaggtatgaa ttaaatgtcc agttggcgca aatgctcaaa ggcggcgtca
    38521 ttatggatgt aaccaatgtt gagcaggcaa aaatcgcgga agaagcggga gcggtagccg
    38581 tcatggctct cgagcgtgtg cctgccgata tccgcaaaga cggcggcgtg gccaggatgt
    38641 cggatcctgc catgattgcg gcgatcaaaa gggccgtatc cattccggtg atggccaagg
    38701 cccgtatcgg ccattttgtc gaagcgcaga ttctggaagc gctgcgtatt gattatatcg
    38761 atgaaagcga agtgctgacc ccggcggatg aagaatgcca tattgacaag acaaaatttt
    38821 cgataccctt tgtttgcggc gcgcgtaatc tgggtgaagc gttgcgccgg atcgctgaag
    38881 gcgcggccat gatccgtacg aaaggcgagg cggggacagg caacgttgtg gaagctgtcc
    38941 gtcacatgag ggccatgaac cgggaaatcg gtcaactggt gcagatgccc gtggaagaag
    39001 tgacaggatt ctgcaaaacc aacggtatcc cgtacgaaca cgcgctcaaa gtgaaggaat
    39061 tgagccgttt gccggtggtg aattttgccg cgggaggcat tgccactccg gcggatgccg
    39121 cgttgatgat gcaactcggc tgcgatggca tttttgtcgg ctccggtatc ttcaaatccg
    39181 ccgatccggc cagacgggca cgggctatcg tcaaggctac cgcttatttc aatgatcctc
    39241 aaaaagttct ggaagcgtcc atggatctgg gagaagccat gccgggcctg gaaatttcgc
    39301 agattccgcc ggaacagatt ctcgccgggc gcggctggta aatctttatg tcactgcgtt
    39361 tagtgagcga taaaccttct gttatcggtg tgcttgattt acagggcggt gtccatgagc
    39421 atctggagca tctggagcgg ttgggtgtcg cctgtaaaag agtaaagcag acggaagatt
    39481 ttaaagacct ggcgggcctg attattcccg gcggggaaag ttcctgtctt tcccggttgc
    39541 tgactatttt cggcatgaag gatgttttgc tggcggcgca ccgccgggga atgaaaatct
    39601 ggggcacctg cgccggagcg atcttgctgg cccaaaacgt tgtcggcgaa gacgcctgcc
    39661 tcaaactgat cgacatcacc gtcgaacgca atagtttcgg ttctcaactg gacagttttg
    39721 ccggcgaagc gttgattccg ggtatttctg ccgatcctgt tccgctgacg ttcatccgcg
    39781 cgcccaaaat acttgctgct caacccgatg tccgcgtact tttgaaaatt gacgactaca
    39841 tagcagcagc ggaaaatgac tccgttctgg tgacggtctt tcatccggaa ttaaccggct
    39901 gtctggccct gcatcagtac tttgcggtta aatgcggtct gaaaaccgca accgggcaag
    39961 ccggacattc ggatccggac tgggatattt caagctggac aaaatatgcc gcccttgccg
    40021 gcagagccgg tgactgacga aaactgtcgt ttaaaaaaat tgcataacaa tcccaaaatg
    40081 cttggtctgc acattgaaac aaaatggtgt tgacaggtca aaatatcttt tttaaagtgc
    40141 ggacatatta aggagcgtcg tgcagaatat tttttccgaa ctgaaaacag attcccgctt
    40201 catccgtttt gtgatgaagg tgggcagtcc gtcacccctg gatcccgacg aacaacacag
    40261 acggataccg gcggtgtttg tgctgatgat catcagcgtc gccacggcgg tttccggcat
    40321 ctatcattgt agcagcggaa aaaatcccct tgtggttttg gacgtcatcg gttttatcct
    40381 ttcccttatc atgctgcttt atctccgtca gggaaaaaat cccggcatcg cgtacggcat
    40441 gatcagcacg ggttttgttc tcttgtgttg tgcggcgact gttctgggca gaacggagat
    40501 gtcgctgatc ttctgggccc tcgtcctgcc cgccggatgt ttttccatat tgggccggaa
    40561 aatgggtatg ttcgttgccg cattcttttt ctttatcaat ctcctgctga tgaatttacc
    40621 ggagcatatg atcctatcaa aaccctttcc ggcatcagtg gctgcccgtt tcagtgttgt
    40681 ttacctgatc ctgacgttta tgctttatta ttacgagtca tcgcatcaga tgctgattcg
    40741 ctacatccgg catgaggaga ataagtttca aaatgcttcc cgatacgatc cgctaaccgg
    40801 gctttccaac cgcagggata ttcttgaaaa aatggaaaat gagcgggagc gtcattcgcg
    40861 tctggaaaaa acttttacgc tcattatggg tgatatagat aattttaaaa tgctcaacga
    40921 tacgttaggg cacgaagccg gtgactatgt actgcaaacg atcgggcgca tcctgaagaa
    40981 tcaggttcgt ggaatcgatt gttcttcgcg gtggggagac gatgagtttc tgattctgct
    41041 ggtggatacc gatctggaag gcggacagcg cgttgccgaa agaatcagaa aaaaggttga
    41101 gaataccagc tttcagtatc agggcaaggg acttgccgtg acgatgacat tcgggttgag
    41161 tatttatcag agcgccgacg aacagatcga cgcatgcgtc aggcgttccg atgacgccct
    41221 tgaggaaggc aagcggcagg ggaaaaacag agtggttccc gtttagtgtg tttcatatct
    41281 gtcaaggaag aggttcaaat tgttttcttc gtcgactcca agccggttat aaatgaaaga
    41341 ttttcagaaa aatggtcgcc agtcccagga aagtcaaaaa accgaaggcg tctgtaaaag
    41401 ctgtcaggaa aatgtgcgat cccagggccg gatccagctt gaggcgcttt aacgccaggg
    41461 ggattaacgc gcccacaagg gtccctgtga aaatactgat aatcatggcc agaccgagaa
    41521 tcagtcccag aatgggtatg cctttccaga aatatgcaat gatgcctatg acaattccta
    41581 cggcgatgcc gttggcaatg ccgacggcaa tttgtctgaa caaggcgcgc ttcgcgttct
    41641 caaaggtaat ttctccaagc gccatcccgc gtacgataag cgtgagggtc tgactgcccg
    41701 cgttgccgcc caatccggct acaaccggca taaagaacac cagcgccacc atcaatttga
    41761 tcgtttcttc aaaaaatccg atgaccagca cggaggcaag agccgttata agattcaaat
    41821 aaagccaggg gagtcttttt tttactgatt cggtggtgtt attgaaaacg gtgtcgtctt
    41881 cacccaatcc ggcgatgcgg taaatatctt ccgatgtttc ttcctggatg gcatcgacaa
    41941 cgtcgtcaat ggtaatccga cccagaaggc gctgttgatt atccacaaca ccgatagaga
    42001 ccagatcgta ttttttgaaa agacgggcca cttcttcctg atccatgtcg tggagaacac
    42061 tgggaatatc ctcgtcgata atatccagaa tcggcgtaaa gcgtttcgtt gtaataatac
    42121 tttgcagagg aacggttccg atcaatttat cggtttcatc aacgacaaaa atgttatgaa
    42181 tattttcgat ttcatctttg gcgtcggcaa cggcctgaaa ggcatcgttg atggtcgcgt
    42241 cttttcttac ggaaaccagt tccgactgca tgataccgcc ggcggtatcg tcggcgtatt
    42301 tgagcagttc ctgaacttcc tgggactctt ccggttcaat cgcttccaga acggccttgg
    42361 cctggtcatc ggataactcc gcgatgatat ccgccttgtc gtccgagtcc atctcttcga
    42421 taatgtcggt gagtttttcg ttggaaagat cttcgataag ctgctcacgg gatgtatcgg
    42481 ataattccag aataacgtcg gacgctttgt ccacatcgag cagcgagaaa agatgtaagc
    42541 ggtcttcttc ttccagttcg tcgatcaaat cagcgatgtc ggccggatgc atatcggaga
    42601 aaagatcgat gatgtcgaat tcccggttgc ggctgatcag atcttttaat gtttccagcg
    42661 cggtcggttc ttttttcttg accttttgca taggatcgcc tgatgttgaa gtcggtttaa
    42721 aaatgatttt gttgattcaa caatgaacta gagttcttgc tcatgcgtat ttacgggaaa
    42781 gaagattctt ttctgaaata ttcgcacgtg aagattcgtt ttccggcagc gagactataa
    42841 ggaaattcaa tacatatgtc aatgtttttc tgaaaaaaag tcataaaaaa caatttttgt
    42901 cataaggttc atccgagccg acggttgttt tctgtatttg aaaatcctgc atccgggtga
    42961 gatttttttc tcgttgacag gggaaatgac ttgcttatat tctctgcggg agttttattt
    43021 tcttggcgca aaggggacga tacttattaa agcggacaac attgaaaaaa atttcttttc
    43081 tgtcggtcgc tactggggaa accttaatgc atcgttgaag agaaaagaat ccgtctggtg
    43141 gatgaatacc ggtgtggctg tttccgatat gaattgggca tggaatgaaa ccccgctggc
    43201 ggccccggat acggcaatcg tttccgaaat cagggaagat tacaaaaaaa ataatttgcg
    43261 tttctggtgg tgggtctatc catgcggaca atccgcggcc acatccgcaa tactgcagaa
    43321 cgcaggattt cgtttatatg ccaatgtctg ctgtatggcg gctgatttaa acgaatcatt
    43381 tccggagccg tacgcgaccg gtgacatgga aatatatcat gtccgcagcc gggaggatct
    43441 ggccgtgtgg gaggacgttg cttttcatgg ttttgagatg ccgcggcacg cgagagaccc
    43501 attcggggcc tttgtttctt cttttgaaac aggagatcag tttccacaga ggtttattct
    43561 gggttgtatt caagggaaac ctgtggcgac gtccctgctt tttaaggatc aacgcacggc
    43621 ggggatatac tatgtatcaa cgctcccctc ttacagaaac aggggatgcg gcttgagcgt
    43681 cacgcaggcc gcgatgcaat tggcgcggga aagcggattt aagcatgttg tcttacaggc
    43741 gacgcccatg gggaaaagag tttatgaacg attgaatttc aaacgatact atcatgccgg
    43801 aatctatacc ttatagaaga tcatgacgct tttatcctct tgacaggcat catcgcgtta
    43861 cgtatagtcc gccagacaaa accccataag ctattcaaaa ttgcaacgtg ctgtcggcaa
    43921 cccatgataa tatctttctg aaaaggacag ttcatgaaaa gaaactccgg tcataatata
    43981 gcatgcgaca atgagtgcgg tggccttcat caatccaacc ccttgtccct tgccaaaaat
    44041 gaaatatcct cgacgctttc ctggtttatg acggataatg cgctatatcc gggtgtggat
    44101 aaacactctt ccccgctgcg ttatgagaac tggtcgggac gatatagcga atatatttcc
    44161 agacaagaag ccgaatccat cgtgagccgc tttatctgcc ctgagaaaga tgaaaatttt
    44221 acactgtttt tcgtcagtga ttataaggat acgtacctgg ggaaaaattt tttaaaggac
    44281 ggcagcgata tttatctgaa acattaccgg atgaccgatg cgccccggga aatccgtctg
    44341 agaaatgttt tgcgccggtg cctcgccaga aaaaacttca atgcttttta tcagctgcat
    44401 caaaaaaaca tcgaagccgt taagcctttg ttctatgtta tgaagagggg ccgctggatt
    44461 cccgatgagg tcatcgctgt cagcaagggg gcgtccggca acaaaacaat tgagaccctc
    44521 atggcggaca gccgggacaa tgatgaattc aggcttctgg ccgtcaatct cggctcgtat
    44581 gtcgccgaac ttcaatccag ggaaattctt tttaaagaat tgtaccggaa tctgatcgcg
    44641 gtcaaccaga cgacgcattg ggattttatt ctctgcgatc tggatgaaat caggtcggtc
    44701 tcggaaaaaa gattttacaa gcacaggaag cacgttgaaa aattacgcaa gaaaataaag
    44761 cgctacggcg attcttttct ccatgctttt gatcaggggt atcagacacg gatgcatgat
    44821 cagacggccc cgtcgagacg gtgatttatc ggggatgttt gccgaaggga tgacgataaa
    44881 aaataccggc aatgggttgt cggttaaatt cttccataca gatcttcaaa acgttctata
    44941 tcgccatcat cacagcgtgc tcccgtttgg acctcaataa tgaccagatg accgtctgca
    45001 tggttcagga cgcggtgttt aacctttgcc gggatatcca ccgatccgcc ggcgctcagc
    45061 gatatgatct tgtctccaac ggttacgtct ccggtgccgg caacaacaaa ccagtgttcg
    45121 gaacgaaaat gatgaagctg tagactcaga ccccctcccg gttctacaat aatgcgtttg
    45181 actttatggc cggcggtctc cgataagact tcaaaactgc cccaggggca ttgggtcatt
    45241 tttcccattt tcatcacctg taagatttgc cgcatatcta acacaaattt tgaacgggga
    45301 aaagcgctct ttttcatttt ccatactcgc tttcgaattt aaattatgat acttacacat
    45361 accgcgcatc gatcatgtcg gcggaaagta acggggtttg atgaacattt cctacacggt
    45421 caggcgaagc agaaaaagac aaaaaaccat gtcgcttcag gtttgcgatg acgcgaaaat
    45481 cgttgtatcc gctccttctt ttacacccgc cgatgacatc agacgttttg tggaggaaaa
    45541 acaaggctgg gtggctaaaa tactggcgcg acagaagcag gaggccgctc acgccaaaga
    45601 gaaaaaatat gaaaccggcg agcaattttt ttatctcgga cagctctatc cgctggaagt
    45661 cttctttgaa ccttttgaga aggaagggat tgctttccgg aatcatcgtt tttatcttaa
    45721 ttgccgcagc gataaagtgt taagaaaaca atgttttgtt gcatggtatt caaagaaagc
    45781 ggacgagatg attcgtcgtc gtgttgattt tttcagcggg atgcttaaac tgccttatga
    45841 aagaatcaga atcacatccg ccggaagccg ttgggggtcc tgttcggaag ataacagcct
    45901 ttctttcagt tatcgcctga tcatggcgcc gccggatgtc gtggattacg tgattgtcca
    45961 tgaattggtg cacatccggg aaaaaaatca ttcgtcgaca ttctggcaaa aggtagaggc
    46021 ggttatgccg tcgttcagag aacagcgtcg ctggctccaa gataaccata gcaacctggc
    46081 actttgagcg gacgaaggaa aggaatgata acgtatcgac atggaacaca ttgaaatcat
    46141 tgatttgaaa caccgggatg gcctggcgga gcgtttcaaa aaaatgaatc tgcggatttc
    46201 cgattataca tttgccaatg tctatctttt tcgcaacatt agtcattacg agttcgtgac
    46261 caaagaatgc ggtttgttcg tttccggctg gaataagcag aatcaaaatt atgtcatgcc
    46321 gttgaatgat ccgcgcgggt gtgacgtcaa aacatttgct gaaattcttg gagcaagaaa
    46381 ttttttcttt cccattcccg aggagtggct tgtttatttt ccaaaagaca aattcagcgt
    46441 cagttatgac gaaagcgaat ccgactatct gtaccttacc gaaaacatgg ccgggtttaa
    46501 gggaaaacaa tacttgcgcc accgcaacca tttaaaacaa tttctggcat cgtatattcc
    46561 cgatggcgtg accctgtcca gccataactg caacgatgcg atgattgttc ttcaccaatg
    46621 gcaggaggat tcccgcacgg catccgacaa aacggacttc gtccaatgca gcgaagccct
    46681 gcaaaatttt tccggccttg cgctctgggg aataatatac tacattgccg gcaaaccggc
    46741 cggttttatc atcggcgaag cgctggcggg ggatacgttc tgtctgcatt tcgccaaagc
    46801 atcgaaacgg taccacggca tttacgaata tatgtttaat gatacggcca aaagactgga
    46861 gtctgcatac aagtatctca atctggaaga ggatatgggt aataagaatt tgcgccggac
    46921 caaaagttcc tacggtccgg agtttttgct gaagaaatac cgtgtcggaa ttatctaatt
    46981 ggaaaaaaag acagcgatga gcgaatcggt taatataaca tcactgcagg aaggcgatca
    47041 gggagtgatt catgccatcg aaggcgggtc ggccattacc agccgctttg ccggcatggg
    47101 gatcgtctcc aacgccaggt tccgcattgc gcagatcagc ggcggcctga ttgtgcttca
    47161 ggttgccaat acccgtatcg cgctgggacg gggtgaagct tcaaaaatca cagtttccaa
    47221 ggtcgctccg gaggccgatg tatgtatgcc gcccatcgaa aaggaaattt ccgtggcgct
    47281 tgtcggccaa cccaatgtcg gcaagtcaac cgtattcaat attctgaccg gtttatccca
    47341 gcatgtcggc aactggccgg gaaaaacggt tgaaaaaaaa gaagggaccc atcgtgccga
    47401 cagcgttttg atcagaattg ttgatttgcc cgggacttac agtctgacgg cattttccga
    47461 agaagaaaga attacgcgtg atttcatcat caaggagcaa cccgggctgg ttgtgctggt
    47521 cttgaacgca acggctctgg agcggggtct ttatcttctg gccgaagtgc tgttgctcaa
    47581 tcggccggtc atcgtggtcc tcaatatgct tgatgttgct gccgatcagg atattgaaat
    47641 caacatccgg gcactggaag attctctggg cgtaccggtg attcccatgg tggccaaacg
    47701 caacagcggc atcagggaac ttgtttctca tatctcctcg attgcgtcca gtgaatacaa
    47761 atttcaaccg gtaaagccgg aagtatccac cgatcatcag ttgatctatc agcaaatcct
    47821 gacgctggtc agaccctata tccatgatcc ttacacaccc gaatgggtgg cggtgaaatt
    47881 gatggaaggc gacccggaag ttacccaact tgtcgaacag caggttcaga ccatcgcgcg
    47941 cgatcaaatt gaaaaactgc tcatcaagca tgaagacgct ctgcatgctg tggtcaacgg
    48001 ccgctacgat tggattgaag aaatcacccg ttccgtcgtt tcccgtttta aaatgggaca
    48061 ggtcgtgttg accgacagaa tcgatcatgt cctgacccgt ccggttttcg gaattcccat
    48121 cctgctggca gtgatggccc tggttttctt cctgacctac agtgtgggtg tcccgcttca
    48181 ggaatggctc agcgaccgta ttcagcaatt tatcggattt gccgaacccc tcacgaccgt
    48241 ttggccttcg tggttgagcg gattgctgct caatggcgtt atcggcggcg tcggctcggt
    48301 gctgacgttt ctgccggtgc ttctgatatt ttttgccatc atggcttttc tggaggatgt
    48361 cggctacatg gcgcgcgctg cgtttgtgat ggacaaaatc atgcaccttg tcggattgca
    48421 cgggaaaagt tttattccga tgtgtctggg attcggctgc aatgtgcctg ctgttttggg
    48481 cgcccgtatc attgagacaa aaaaagccag aatgatcacg ctgcttctga ttcctttcgt
    48541 tccctgtacc gcgcgactgg ccgtgctgac gctggtatca gcggccattt tcggtcaaaa
    48601 cggcgccttc gttgcgtgga gtcttgtggc cctgaacatc gccgtgctgg gcattgccgg
    48661 cgtttttgtc aataaaacgc tctggaaaca ggatgcgcca ttcatcatgg agctacccat
    48721 ttatcacacc ccggatgcca aaaccattat gatggtggtt tggacaagaa tactttcatt
    48781 tatccggaaa gccggaacgg tgattcttgc tgtttcgctg attgtctgga tgctttcgta
    48841 ttttccgaat ggaaacgtcg aagaaagtct gctggcctcc ttcggcagac tgcttcagcc
    48901 attgggcatt ccactgggac tggactggaa aatgattacc gctcttttga ccggtctggt
    48961 ggcgaaagaa aatgtcgtgg cgactctggg cgtgctttat tccgtgggca gagacggtct
    49021 gtccaatatg ttgccggcca ttatgagtcc ggaatcggct gcggcgtttc ttgtggtcat
    49081 gatgcttttc atcccctgtg ccgccacaat tgccgttttg aaaaaagaaa tgaacaacaa
    49141 taaatggttt tatactgcta ttgtcatgac gctgctggtg tcgtatgtcg gtggaatcgc
    49201 ggcttataat ttcgtcaaat ggttgggtgt ttaatcaatc aataaccgat ttctctaacg
    49261 accgggcgat agagaaacat attgcggcgg ataaagctgt cctgcttctc catatcttct
    49321 gatgaattac agacgtaagt caccacaaga tatttccccc tggaaaatcc gatgtgttct
    49381 tttcccgcgt aacaaaaatt tcctttatcg tacagagcgc ttcggggatc cacttccggg
    49441 acgggagcgc ccagaggttt cgcttcactc cacggacctt ccagtttgtc ggaggtctga
    49501 tacagcaacc ggtccccttt attttgggtg cttaaataaa cggcgatcca cttttgatca
    49561 tcttcatggt agcggacgct caattccgag acactggcgt cgagaacgat tttaactttt
    49621 tccgggctca gccggttttc ccaggttcct tccttggtgt aatattctat cgccccggca
    49681 ggacgatcga ttctgtcgac aggaatgcgg gccagaatat ttccgaagac attcaaatcg
    49741 tttttgacgt gaacatagaa agggtagaaa aaaacatagc gatcatgcac aacggatgtg
    49801 gcggcaaagg cggcaatttc cggaggcagc gcgtgcgtca aatcgacgta atcataattc
    49861 caattgcggg gatcggtcgc ggaaaaatct tttacccgga cgaatttgtg accggcgact
    49921 ttgaaattga atacctcatc ttctttgtct cccggaacaa tcgatatcag cggaatatat
    49981 aaaacgcggt taaccataaa cggatcctgc ggccagagcc attcattctt gccgaacgat
    50041 gacacatatt taccgccgct gtgttgcaga taataggtga tctcaaatcc gcgattttcc
    50101 gggcaggagg aaatcgcaac ggtggtgccc aggacgatgt ccatatcaat gcggtctcgt
    50161 ctgcatcccg ggcagccgac aaacgtatct ccgaaaaccc agagtgtccg atccttgtcc
    50221 agtttaatgg agtaggcgcc gtcgccgccg taccagccat ccctgtcggg aaaaaccggc
    50281 aggcaatgac ccggcgagac gtccgcatcg tatcttgcgg cagtaaacgt ggagcaggaa
    50341 agaagaggaa taacaacgaa caggaaaata aagccccggc agacattccg gatcaaaata
    50401 ttttttttca taacatcaca tcaaatggtc aaatagaatt tttacgccaa tcacaattaa
    50461 aacaattccg ccgaagattt ccactttccg gccgaataat ttcgccagcg ccttgccaaa
    50521 aatcatgccg gtagccgtca tgacgaaaca gacgacgccg atgacgacgg cagggaacaa
    50581 aatcggatgt ttcaagacgg aaaaactgaa acccaccgcc agagcgtcga tgctggtcgc
    50641 aatggagagc atcagcagcg gccagccggt tgtccgatcc gattgtgttt cgtcaatgcc
    50701 ttttccgcaa ccttccagga tcattttccc cccgatcagc gccagaagtc cgaatgcaat
    50761 ccagtgatcg aaggaggcga taacctccac aacggtcaga ccggccatcc agccgatgat
    50821 gggcatgata aactgaaaga atccgaaagc aatcgcgagc cgcaacaccg ggatccatga
    50881 tcttttctga tgggcggcgc cgataccgat cgcgacggaa aatgcgtcca gtcccagtcc
    50941 tgtcgcaatc aagaatattg ttatgaagcc catgacggtc catcattcat tctgaattat
    51001 tttcagagcg ttaaagattt tttctgccaa aaccttacca attcccggga cgttttgcaa
    51061 ttcttccttc gttgcatctt ttatttttcc ggcagagccg aaatggctca gtaaaatttt
    51121 gcggcgtttt gcaccgatgt cggcgatgtc atcaagtacg gaatgcaaat cgcctttatg
    51181 tttgatctga tgatggtaac gtaatgcgaa acgatgggcc tcatcccgca ccagttgaag
    51241 aatgcgcaac gcctgcggcc aggcggataa atagagggca tctttgcgtc ccggcaggta
    51301 gacgcgatcc tcccacgtcc gggttgtctt ttgtcccgcg cggacggtac agcgccggca
    51361 tttttctttt gccaggccga tgacatccgt cctgatcttc agatctttca gcaccgaaag
    51421 cgcgacgttg agttgccctt tcccgccgtc gacaacaacc aggtcaggca ggtgttcacc
    51481 gctggaaaac cgtcgggaaa gcacttcctg catcatttta taatcgtccg gttccgccgg
    51541 cgccgtgacc cggtaacgcc ggtaactgtt tttgtcgggg tcaccgtccc gaagaaccgc
    51601 catggaaccg acggcgtatg tgcccccgat attggaaata tcataacatt caatacgtcg
    51661 cggcaatccg gcgagtgata atttttcctg cagtatttgc agggcgaatt ctttttcttc
    51721 ccgcttctta taagtggatt cccagaggtt gcgcgcattg tcgttggcca gctgaagcag
    51781 ggcgtttttt gctccgatcg agggcaccgt caggatcatt tttttatttt tcttttcact
    51841 cagccattcg gcgatgacgc cttcatccgg caaatggcag ggcatgatga tttcgtcggg
    51901 caacgtgacg tcaccgtcat aatactgcgt caaacatgag gagacaattt cgctggcatc
    51961 gattttcaac ctgatcggag cgaatgattt gctgcccagc agttttccct ctcggacgaa
    52021 taaaacacag acctggtaat gatgatgtcg gctgaaaaaa ccgaagacgt cctgatcctt
    52081 ttcttctccc cgttcaacgt tttgtttttc cagcgcgtgt tccagcgcgg ctatccggtc
    52141 gcgcagacgg gcggcttctt cgtaatgcaa ctcttctgac gcgtcgttca tctgtttttt
    52201 cagttcggta atcagctccc ggcttcgtcc ccgcagaaac gatacggcgt tgtcggccag
    52261 tttccggtag gcctcctcgt tgattcgtcc gctgcaaggg gcaagacacc ggccgatctg
    52321 gtattccagg cagggcctcg ggcgaagctt aaaatccctg ttgcggcagg aacgcaaagg
    52381 aaatatctgc tggacgaaac gcagggtttc tttggccgcc agtccggaag ggtaaggacc
    52441 gaaataaagc gcagcgttat gtaatcgttt gcgcaccagt tgcagacggg gaaatttatc
    52501 cgtcggatcc agcgacaggt aataataggt tttatcgtcg cgcagcgtaa cgttgtaacg
    52561 cgggcggtgg cgcttgatta aattgttctc aagaatcaga gcttctttag ccgttgcggt
    52621 gttgatgaat tcgatgtcat aaacgcgcgc cattagaaag ggcgtcatcg gacggctgtc
    52681 tttccccgtg aaataagacg ccacacggct ttttaaatga tttgctttgc cgatatagat
    52741 aattttcccg gccggatcct tcataaggta gacgccgggg ctgcgcggga aaagagcgat
    52801 tttatctttc aggacgttgt tttctttcat catttcagaa ggcgacgacc attttgcacc
    52861 ggtcgtcagc cgtgtctgtc ttagagtttc gggttattgt atcagttcct gaatcagaag
    52921 caaagcggct ccgccgattc ccgcggttgt gattaaaaat accaacgtgc tgacgatacc
    52981 gaaaaagctt ccggcttttt tattgacgtt cggtttcacg gcaagaaaca gaaaaagtga
    53041 cggattggcc agcgcccccg cacatgccaa tacagcgaca acgagaggca ggttaatgcc
    53101 ggtggcaaaa agcacgagca ggaggatgcc cattatcaca taatcaacgt gagccttgat
    53161 aagcttttct tcgtcgcgga tgattttctt catgacggtc agagataggt atttgcccgc
    53221 gataatgaac cagccggtcg ttatcgccag cagaattaat acggccccgg ttatgatgag
    53281 atatttcatg atattacctc cggaagatat tagtgtcagg tcttattatt catggatggc
    53341 tgttttttat gttgtccatc agccggtcac agatagccag cgcaattttt ttattggagg
    53401 tatcattgac gtggatgtaa tcggtgaaga cagcatcccg ggaattgcag aatatgtcac
    53461 tgagatcatg aaattcaaag ggaaaccgtt tttgctcttc ccggatgttg cggagaattc
    53521 ttttccggac gtcgttcgta tatggacgga caatgcccgc ttcttccggc ggccatcctt
    53581 ttaaaatatt tttttccgta tcggaaacgg cgaaacgttt atagaagatt atgggttgca
    53641 gaaaggcaac atattcaaca tggtaggcgc ggcagatttg tcccgctttt gccagattgc
    53701 cggcataaat tccggctatt ttttccgacc aggcatattc tccgtgttgt gccgaaagtc
    53761 tcaggttgga aaaattgccg aatttttcaa aaaaataatt gcggaacacg tgccggagca
    53821 tttcgctgcc gtatgcgagc agcgcgggcc aggggtaatc tcctgttgcc tgaaatagaa
    53881 cattgttttc ataagcaagg aagttaaagg gatagccggg ccggggatcg taaagcagag
    53941 ggtccataat atcgttgccg ccgtcgtaga acaccaccat gtccggttcc gagtccaaca
    54001 cgtgaaagac aaggtgagcc agttcctggc cggaattctg cgatatgacg ccgtagttga
    54061 agacgcgcac gttctcgaat ccgcggcgct tcagttcctg ttcgacatag acgggcaacg
    54121 gcggattgcc gtattcgacc gtcgaaccgc cgagcataaa tattctgaat tctccccgcg
    54181 ttttggccga ttcttggctg ccgtatgcga atcgactgaa catcgtgtag ggatttgcat
    54241 aacggacatc gatcctgtcg tcaaaagggg caattttagg ataaagatac cggaaaaaca
    54301 attcggcgac caaaaaagaa atcagcagaa aacaaagaat gaagagaaat cctttaagcc
    54361 gtctggacat gatggctcct gccgcataaa atcaaccagg atgtatggtc tgttccggaa
    54421 aacattactg ccgctgcctt ttttatgttt ttcccgatgt tccgtattcg gtgaattgcg
    54481 ggatgtagtt tttaaacatt ttccgtattt caggattttt ttccgtgtca taaagccgtt
    54541 ccataatgcc tcttacaccg tgcttcgacc gtacaagaga ctcgccctgc tccttataca
    54601 gggcaacggc ggtctgggat acagccgaac agacggcgtc tatgaacctt tgccagaaat
    54661 gcaatccctg ccctttattt gtggggtgca acagagaccg gaaaaaaatg atggacgagt
    54721 tgaacgttgc cctcaccggt gcgccgacta ttttcgatat ttcatagacc atggacgcaa
    54781 taatcagcgg actttggtgc agcgaatcgg gcggaatttt gttggtttcg gccagatcgg
    54841 caacggaaag ggagagaaaa taatccatcg tgcctttttt gccgtaaatg aacgtattat
    54901 tctcctggct ttcatggaaa aagaccgagc cgggcaagtc aacgatggtg ctgggctgcg
    54961 ctccgattcg gtttcggata cagaattttg taatgtcaac gccggtgttg tcggttggag
    55021 aattaaaata gtcatagggc aggcccacaa taaaagatcc gtgaacggaa atgccatgag
    55081 catgaatctt cctgattttg gatgcgtaat atttttttac cgacatgccg cttttttcga
    55141 tatctttaac aatatgctta ttgatatgct tcaaattgcg tatatcgagg gattccagac
    55201 cgatgaagaa atgggtgcct cctgatgctc tgatcagttt tagtaattct tcgtcgtcgg
    55261 caacttcaat ggagatttga accgcgtagt taatgcgcag atcgctggca atgattttct
    55321 ttaagaagga tatcagattt tttttattgg agaacagatt gtccggcgtg aagaaatagt
    55381 accggttttt aaaatcaacg atttccttct gataagaacg aagctcctca acaaagtcgt
    55441 ccgtgtcccg aaactggacg attttatttc tctgcggcag agtcgatatg gagcaaaact
    55501 tgcaggagaa tatacatccg gtgaatggtg cgacaggatt aatccggaaa tctttcaggg
    55561 gggtgtattt gatcaccggc agtctgttga gcagattgat tttcagcggt tccaggggct
    55621 caaggttgtc aaaatttccc cagaccccgt tttcaatcag ggtttctccc gaatagtccc
    55681 ttttcaggga atgcgttttc aaatcatcca ggatggtctt gaggaccgtc gaatcgcccg
    55741 cgcccctgac aatggaaatc agttcgggat agggcgcata ttttctgatg aacgtgtccg
    55801 catcttcagg catggtggaa acatgtatcc cgccgatgat gacaggaatt tttgcgtgat
    55861 tcagcacgat ggccgcagca gcggcggctg gaaaactgga gctgatggtg gaaataaaaa
    55921 cagcctgggg aggggccttt ttctgtctga tgacttctga aaaattccgt cgcttaccgt
    55981 ccaccatgac tacgagatga tcctgggcca gttttgccag ctgatgctgg atatagatgc
    56041 ctgcatacat ttcgatggcc agggcgtcaa ccatggttgc ccgctttttt tccggctgaa
    56101 aatcgatgaa tcttaaataa ttttcagcat tcacttcgtg cgagttctgt ccggacagat
    56161 aggtaatcac atcaaacggc gtgatggaat tggccggcat cagatccatc aattgatagg
    56221 tcagcggatt ataaacgata aaataattta cggacattgt ttaattgagc cctttctcat
    56281 attttcagta aaaacattcc agcccatata cagaaccatg gggtatatcg tgaggacgat
    56341 gcccattgaa gcggacacgt atccggattt gctaaatcgc cagtctcaat gtctttcttg
    56401 agcttttatg atttttattg tgcaagacat agcacagagt ggtttcctca aacaagctta
    56461 attttaatca tcctttcggt ttttttattt gtcttgcggg aaaatgttta ttttttgttt
    56521 atccccggtg cctgcgtttt caaaagatgc ttgtcatgca acgtggctaa caggaggagc
    56581 gacgccatcg aaccgatcag acataaaaac atgtcggttt gggtatccca gagatatccc
    56641 tgggttccca gaaaagcttc cgtgtcgttg gggttggaga gcgatgcggc ccattccaga
    56701 atctcatata atgcggaaaa ggccaggggg acggcaaggg agaaaaaagc cagccatttt
    56761 cccttttgga ccggtgaggt tctggcgata atttcacgcg cgtatatcgc cggaccgaac
    56821 ccctgcatga gatgcccgat tttgtcgtaa ttattccgcg tccagccgaa ccattcttcc
    56881 atccagaggc ccagcggtac tttggcgtac gaataatggg cgccgagcat caggatcatc
    56941 gcgtggaggg cgattagcgt ataggttaaa tccgtcagtc tgaatttctt gtatgtcaag
    57001 agtatgatcg gcaaggccat catgaaaggc agggtttcca gaaaccatgt cagataggtg
    57061 tcttgcggtt ttatggccga ccagatccag acgatcaaga cggttgccag caagatcaga
    57121 tgatatgtac gtacgggcat attttcttac ggtcaatgtg cgtgaagaat tttactgcaa
    57181 tctacttcat tcaacggaaa aaataaagaa cgatttatgg tgcttcggac catgatgatt
    57241 ggagtccatt atttcttgat tttgtgatac atcaccatac agaacataaa aaagttgccg
    57301 gagcgtaaaa gacacaggat gaaaaaaatt ttccttcaat tttttctgct gctcgtttta
    57361 ttcggtttgt taccggttgc cggaattttc aatccggttg ctgcggaaga atctgcacgg
    57421 aacgcctccg ggaatcaaac ggaaatcgat ctgatgcttc tgtttaccaa cgatttacat
    57481 tcttactttc tgccgcatcg cgttttaacg gaagacggac gacagacagt gcagggggga
    57541 tatgccagac ttgcctgtat gatcaacgag cagaggcgcc tgcatcccgc taaaaccttg
    57601 ctggtcgatg ccggcgattt ttcgatgggc actctttttc ataccgcgtt catgaccgaa
    57661 gctttcgaac tgagactgat ggggaaaatg ggctatgccg caacgacgct tggaaatcat
    57721 gatttcgact ttcatcccga gggcctggca aagatgctga cagcggcgcg ttccaaatcg
    57781 caaatactgc cgcagattgt tgcctccaat cttattgtga acggtgacca tccaggcgat
    57841 aaaacgctga agcaggcact gaacgattat ccggtccggg attacatggt tcttgaaaga
    57901 aacggtttac gcatcggcct gttcggcatc atgggaaaag acgcggccga cgatgctcct
    57961 ttcgccaaac cggtgatgtt tgccgatccg gtggaagcaa gcaaaaagac cgttgcgatc
    58021 ttgaagaacc gggaaaaggc tgatctggtc atttgccttt cccacagcgg aacgtcggcc
    58081 gatgcgaaga aatcggaaga tgaaattctg gcccgggcgg ttccgcaaat agacgttatc
    58141 gtcagcggac atacgcacac ggtcttgaaa acgccgatca tcatcggccg gaccattatt
    58201 gtctccggcg gacgctacgg caggtacctc ggatcgctgg gcattcgttt tgaccggttt
    58261 aagggcgctt cattggcttc atacgatcta agacatgttt catcagaaat tcctgaagac
    58321 aaggcggttc aggaggcaat tgccggctac gaaaagatag tcaatcgcga ctttcttgca
    58381 cactatgacc tgacattcga agacgtaatt gccgaatcgg atttttccat ggaatctctt
    58441 gaatccatgc atcgtcatcc gcgtgaaaca ggactgggta atttaatcgt tgatgcctat
    58501 cgtgaagccg tcaaacaggc ggaaggtccc gattaccgcc atgtccactt tgccctgcaa
    58561 cctttgggcg tgattcgcga ttccctgctc aagggaaaaa ttaccgtaag cgatgtgttc
    58621 cgggtccttt ctctgggaat cggaccggac aggattcccg ggtatccgct ggtttctttt
    58681 tatatcagcg ggaaggaact caaggacatt ctggaagttc atacaaccat agcgccgatg
    58741 cataagaaag acgcttattt gcaggtgtcc ggcgtgaaat ttacctataa tccgcggcgc
    58801 atctggttcg accgggtcac gtcggtttgg gttgcggatg aaaaaggcgt ttatcaaccg
    58861 cttgatcaga caaaactcta ccgcgcctgt gcgaatttgt ataccgccgc ccttattgat
    58921 tacgtcagct cggtcagttt cggtttaatc catgtggtgc cgaaggacag ctcagggaag
    58981 cgattgaccg atctcaactc gtccatcgtg tctatgaaaa aaaccggcga aatcaaggaa
    59041 tggctggccc ttgcacttta tctgcgttca tttcaagaac cgcaacattt gcaaacggcg
    59101 aagattccgc ttaaatacag caaaccggag ggacgatatc tcgcgcagcc gtcctgcaat
    59161 ccggtcagaa tttttagcaa aggcaatatc cttacaaagg tttttctggt gtctgtaatc
    59221 ctactcctta tgatttgcgg tttaattgtc tatgtttttg tcaaaaaaat gaaaaaaatc
    59281 agcaagaaga acccccgata aatgtcattc ccctgattaa aatagatatt gtctgtattt
    59341 acaataattt gtagaaatat tttattattt tgacattcat tactgccaaa aaaaataaaa
    59401 atacttgatt tttgaaaatt atccacgtac aattggtcca atggtactac caatgtttat
    59461 attttttgac acgctaaaga tcgttttttt gatggtgatc cctcctcaaa gatagatttt
    59521 ccaattgcgg tgccagaagg tgaagaagac catgcagata gaattaacgc ccgttgagaa
    59581 aatgaaggct ccggagcaga ttgccgagag gctcctgaaa tatattctcg gaggcggcgt
    59641 cagtcccggt gaaaaattgc ctccggaaag gaccctagcg acacaattaa atgtgacgag
    59701 ggcgacactc agggaggctt tgaaaaaact cgagcagttg aaactgattg tcattcatca
    59761 gggcaagggg attatcgttg aggattttca caaggcctca atcgacctga ttttttcatt
    59821 gctggtcatc aacggccaaa ttgatctcaa gattctggaa aatattctgg aagcaagaga
    59881 actgttcggg acagacgttg ccagatgcgc agcccggcga gccgacaaaa aggatgtcga
    59941 gcagatgaaa attctgatga atgaactgat caaggcaaca gatccgggaa aactccagca
    60001 gctggatttc gaatttttcc ggcagctcgc gctggccagc aaaaacatgg tgtacattct
    60061 tctgatgaat accatcaaaa caatccatga taatcatctg gatctgtttc tgcccctgtc
    60121 gacgaatctg gatacaacgc ttcagcagga aattctgcag gctgtgatag acggcgatga
    60181 agaaaaggcg gctgaaaaag ccaacacttt tctcaaagcc ggtatggaat tactgaaatt
    60241 tttaaagtcg ctttcagaca caaaggagtc ccaaaatgca gaccgcgaga caaattgaca
    60301 aagacccgat cgaacaaacc caaaaactca acgttctgaa aatacagcgt acctgtgttc
    60361 atgacggtcc cggcatccgc acaaccattt tctttcaggg gtgcggactg cgatgcgtat
    60421 ggtgtcagaa tcccgaagcg ctgtctttaa aaccgatgcc ctctcccgcc gtgcattatg
    60481 caattgctga cgtcctggag gttgtcctgc gcgacaagga ttattacctc aagaccggcg
    60541 gcggcgtgac gctcagcggc ggtgaacctc tgctgcagga tccggaaagc cttacccatc
    60601 ttttgaaaaa attgaaggac gaaaagattc acattgctgt ggaaacctcc ctgcatgtgc
    60661 cttgggagaa tatcagcaag acagcgccct atattgactt gttcctgatc gatttgaagg
    60721 ttgtggggga tgaggacctc cacagaaaat gcacaaagca ggacagccgt ctcattcacg
    60781 acaatattag aaaactgctt gcgtcaaaag cgaacattaa attccgcatg gtcatggtcc
    60841 cggaatataa cgatcacgag cagcacataa aggcgacggc tgattttctg aaatccgtaa
    60901 accacaaatc gattgaactt ttaaaatatc acaatatgta cgaagacaag gccaagcgtc
    60961 tcggtctggt ccgtgaatcg ttgaatattt caccggagca gagtttgacc gccgttaaaa
    61021 aagccgtcga attattgaaa ccattggata tttacgccga gtgtgaagat ctgggaaact
    61081 ttcgcagtaa agcggttttt accgatcgtg ttcatgcgat acaaaaagat atacgaaaga
    61141 gcggttatca tctctgtttt gaaacatcca aactgaaaac aaaattttac agaaaaaacg
    61201 gtttcaaaaa accgacccac atccatcgcg cggaacgtct cgattatgtg ctgaaaaata
    61261 agcaggtcag gatctatccg catgaactgc tggtcggtaa ttttacatcc aaaagagtcg
    61321 gcggtcaggt gtgggaggaa cattacggga ttctcttttc atccattctc catcagattc
    61381 accgccagac accggtggcc ttccagtgtt cgtggaaaga cagactgtat ttttattgca
    61441 gaatattgcc gttctggcag aaacactgcc tgctgaccaa agtcaataaa acgttgtccg
    61501 atctggtctt aagcatttca cggttatgtg aaatgaatgt cggattcaac aacaatatgg
    61561 cagcgatagc gcatttcatc gtcaacttcg aacgtattct gacacttggg acgaccggca
    61621 tcatcaaaga acttgaagag gcacaaaaag aaaagcctca gaatgatcag accttttatc
    61681 agggcgccat aattgccgtc aaagcgctgg aaaattttgc actaagatac gctgacgctc
    61741 tttccgacat gagcaaaaca gaagcaaacc ccgtccgccg taaggaactg gaagacttgg
    61801 ttcagatttg ccggcatgta ccgaaacatc cggcacgcac gtatcatgaa gccctccagt
    61861 ccatgatgtt tctgcaggtc gcgctctgta ttgaatcgta tgagaacgcc atatcgttcg
    61921 gacgtctcga tcagatattg tatccttatt ataagaaaga caaagaggcc gaaatcatca
    61981 catacgaaaa agccaaggaa ctgctggcct tatttatcct caaaatggac gaggcgattc
    62041 tggtaaacga cggagatacg tattttgcaa ttggccggct tttcgaaacc cagtccacag
    62101 accagaccgt gacgttcggc ggtgtggaca aagacggtca ggatgcgacc aatgatctca
    62161 cctatatgtt gattgatatt tgcgaactcc agtttctcgc ggtggatatg tcggcaagaa
    62221 ttcatccgaa cagccctgag cgctatctga acagaatagc ggaggtctat attaacggtt
    62281 ccccgcaacc gaaactgaat aatgatgaag tttatattca atcgttatca aaacattatg
    62341 acacgtctat cgagaatatc agaaactatt ccattgtcgg atgcgtcgaa cccaatgcgt
    62401 ctgatgatca tttcgggaac accgactgcg ccaatatgaa tgtcgtcctg ccgttgcttc
    62461 aggccctcaa aggcgaagaa gatgatttat ggaactacgg cttttccgat caaatggaaa
    62521 aactgacgac gaaattcatt gaatataatt tccgcggcgg caacagggtt tccaaatgga
    62581 tagcggacaa atacggcaaa gcccgggcct cctataaaac gaaaaagaaa acagcatata
    62641 atcctccggc gaatatggat gaattgttgg aacgatatca gaatcgtctg aatcatctgg
    62701 catcttccat attggctgat catcagaaaa ttgaaaaaga aatatgcaaa aactttacga
    62761 cgcccctggc ttccgcgcta tccaggggct gtgtcgaaaa aggactggat gtgaaccagg
    62821 gcggatgcga gttcaacacg agcggtatcc aggccgtagg catcacggac gccgcggatt
    62881 cgctgcacgc actcaacgaa gtggtcttca agaaaaagat gtattctttg atggacgtca
    62941 tccatgccat cgataacgat ttcgaaggtg aagaggatga acaaatacga caagcgctgc
    63001 ttgccgtgcc gaaactggga catgatgatt cccccgaagc ggtcaaatgg gtcaacaaga
    63061 cgctggagat ttattgcaat gccctgaggc aggttccgaa ttcgccgcgc aacggattct
    63121 ataccgccgg atactatgcg ctgaacgtca gcgatgttta cggcaagaaa acaccgtcgt
    63181 tgccgtccgg ccggctcaaa ggggttcccc tggccaacag tatttctccg cactacggca
    63241 tgcagatgtc cgatcttctc tcctcactga attccgtgtc cggtgttgat tttgtaaagt
    63301 atgcgcccaa cggaaccacc gtcaccttta ccatggactc ggctcttttt caggggagag
    63361 acggcataac aaatcttgcc ggtattttca gcacgtattt caaaaaagga ggcatgcagt
    63421 ttcagcccaa cgtcatcaac cgggaaatgc tgctggatgc ttacaaccat ccggagaaat
    63481 atccatacct gctggtacgg attgcgggat actgcgcata cttcaaccat ttgtccgatg
    63541 atttgaaaaa agtcattatt aacagaacct gttattcatg aacaggaact gctgaaaagg
    63601 gcttgttccc ttccggaagt gaaacgcaaa cgagtacatg gacctgtttg cgatcccggt
    63661 cataggtgca agtttggttt catgatgccc tcgagccgta atctttggcg ctcgaaatct
    63721 tcctcgctct ctcccgatgc cagatccagc atgtccccga aacgcagggt aacgtgcgca
    63781 aaaggcttgg gcagcatgaa atgatcccag ctgttgaaat accatgcacg atcggcggag
    63841 atatagaacg gcacgacgag cgctccgctg gcttgtgcca gtttaacaac gcctgctttc
    63901 acgatgcctg ctgggcctct gggtccgtcc acaacgtgac cggcaaaacg ggattgaatc
    63961 agataatcaa tcattcccct gagcgcacgg ctcccgtctt ttgatgatga tccccgcacg
    64021 gtatgccagc ctgttttctc ggcaatcctg gcgatgatgt taccgtcttt actctggctg
    64081 atcatcagtg cgggatgata gggggcatag gttttgaaat ggcggatggc tgaaaaaaac
    64141 tgctggtgcc agcagcaaat cagaatacgt cctccctgtt tgaggtgatc catccagggt
    64201 ttttcattta caacttgcag gcggaatgtc caactgtaac cccgtataaa ataataaaga
    64261 aaatcctgga gaaaatcggt taatacatat cgatattttt tcagcatatc tatgtccttt
    64321 caggttattg gtaaaatcaa ccggtcatga acggccggtt tgggaatcgt ctgactccgg
    64381 tgttttttat gatggcgaga caaaagcccg tcgtggccgg ctgaaacatc ccatgcgcga
    64441 ttcggagaca ttatggattc ccattccggt ggttgtctac ggccttgcag accgcgttat
    64501 taagtttttc attaattgaa ttcggaatga tatcatgcca tccgtcattg gtttccggta
    64561 cggaaaaaag caacgtgtct ttttcatcat aaatatttat ggaaacaatt ctatatttgc
    64621 cctgcaggca gtccaattcc agcaatatcg attcctgatt caacacagca gggtttcccg
    64681 gcgccttttc ctgctttagc agcatggcat aagcgtccgc tctgccccgt tcgttgtaaa
    64741 tggttacggt ccagacctga tagatatgat cactgatttt tctgatgctg ttcttgtcat
    64801 aatagccggt gccccgggga agcagtcctc tgaatcccca gtccgccgca agactcccgt
    64861 atgccgataa aagcaatgcg gctaaaataa cgatgcaata aatctttatg gttttcatgg
    64921 ttcgatgcaa taaaaaaatg ttccggatgt ttttcgggag aatatttttt tatactttta
    64981 aatgcaagga gtttttgagg tcgctaacgc cagtggaaca gtttggggct gatttcgctg
    65041 cgtttcttaa cgccgagggt atgaaaaatt tttttcagat gatcttttac ggtgtattcc
    65101 gtgatgcaga atcttcctgc aatttcccgg ttggagtacc cggtcgccgc caggaaagcg
    65161 atctctttct ggcggggggt cagatcgagt attgtgagtt tctccctgat gcatatggta
    65221 tccggcattc tttccatgac gatcagtttc aggggagacc gccggtggtt gttaatattt
    65281 ttaacatgat aatatttggc cccgatgctg aggtaggtgg aatcattttc cgccagtgtg
    65341 cgaagcaggt tatccagcga atgagttttt aaaaccattt tggcctcttc gttcatgtaa
    65401 cataaagagg gctgtttctt ttgatggttt cttattaaaa caacgccggc gcccttaatg
    65461 gaattcaagg atttgatcag agcgtcttca tgacttatct ttgcggccgg attgagatcg
    65521 gatatatccg ttccaaccgt cagccagtat acctgatttt ttatctcaac ggggagggtg
    65581 ttaaccaatg cgcatcgcag atcgcctttt ttatccctga cggtgatggt aatatttctg
    65641 gcaaaacctt tttcattaat ctcttccaga attttcaatc tttccagact ggtggcgtat
    65701 gcgatgtcag cggctgtgtt cccgatgact tcttcgcgtc gccgtccgaa aaattccgtg
    65761 aaagattcgt tgacgtcaac atacattcca tctgtagccc gggtaatgga catgggattg
    65821 agagactgcc agaataattt attcgtgaac tctttcggaa tatttatctc tgtttcttct
    65881 ttcatgttct catatttctg caatatccgg atcatgctgt tattaaatta attaaattat
    65941 taatatcttt tcgattatct ttaattatgc tgctaaattc aatggcgatt acgctgtaat
    66001 tgttaattat aaaagatgta gatataatac ttatattttt caattcttgt tttcaggata
    66061 gttttgaggc gcgtaagaat tgcaataatc cttcagtaag tctctgcata tcaacggcag
    66121 aaatcatcgt atttttatct gcaaggtcag gagcgaaaat tgatacaggt aaggcagtat
    66181 gcattatgtg ttatgtctta tcaaaacata tatttatttt attttttcgt cgtgatatac
    66241 ttcaaaaata ttttataaac ttatttagtc atttattgtg aaaggagtct gtcatgaaaa
    66301 agagaatcat ggttttatta agcattatcg tatttgtcct cgggctcgtc tgcattcagg
    66361 gttgccagca gaatgaaaat ccaaaatttc agaatgaata tcaggcggtt ttacttacca
    66421 cgggtcaggt ttttttcgga aaggttgaat ttgtgggtac agattatgtt cttctcaaag
    66481 acgttttcta tattcgcagc gtcgctaatc cggatacaaa gcaggttagc aataccctga
    66541 taagaaaagg caatgaaata cacggggcgg atcagactta cattaatgcc cgtcacatca
    66601 ttatgattga gccggtatcc ccgcaatcac ctctcgccgg ccggatcaag gaactacagt
    66661 cccagaaacc gcaggggcaa ccgtaagttt ttaaccgcgg ttccagcact attgttataa
    66721 caggtgtcag ggtgcggtaa ttctgataaa aaagcccgct gcaatcggca gcgggctttt
    66781 tttataaaac acaacctttt ttacttcaac ccggcggcct ggcgaagcgc atccacccgg
    66841 tctgttttct cccaggggaa gcctttgcgt ccgaaatggc cgtagttaca ggctttgcga
    66901 tagctccaac ccttcggcgt cgtgagtcca agatccttaa tcatcatggc cggacggaaa
    66961 tcgaaaattt ttccttcagt gagaattttt tcgatcttat tttcaggaat tacgctggta
    67021 ccgtacgtgc agacattaat gctgagcggt ttggccacgc cgattgcgta tgcgatttga
    67081 atttcgcatt tgtcggccag tcttgccgcg acgatatttt tagcggcgta gcgtgcgtag
    67141 taagcggcag aacgatccac tttggaagga tccttacctg aaaacgcgcc gccgccgtga
    67201 gaaccgaccc cgccgtaaga atccacgata atttttcgtc cggtgacacc ggaatcgccg
    67261 gccggtccgc cgattacaaa tcgtcccgtc ggattgatga aaaattcagt cttgttggtc
    67321 agcatgcccg tcttgtcgag aacctgatgt gcgacgtcaa tcaggtcttt gcgaattctt
    67381 ttcagcggaa cgtccagcgt ttgatgcgag atgacgacgg ttgtgatttt caccgccttt
    67441 ccgtcttcat gaagaacgct gacctgggat tttgaatccg gccgcaggta atctatcaca
    67501 tttgctttac gcaaaatggt aagttcttcc aataagagat gtgcataata aatcggcgca
    67561 ggcatcattt gcggcgtggc atatgacgca taaccgaaca tcatgccctg atcgccggca
    67621 ccctgatttt caggtcgatc ccgatcaacg ccctgtttaa tatcgggcga ctgaccgtga
    67681 atggcggaaa ggtaattaaa tgtatcccaa ctgaagtttt cacagggatc atcataaccg
    67741 atgtcctgga caacttgtcg cgaaatggcc tgaggattga tcttgttcca gtttttacag
    67801 gtgatttccc caagattaac gacaagattg ggcccgacag ccgtctcaca gccgacatgg
    67861 gcgtgtttgt cctgttccag gcaggcatcc agtatggcat cggaaatctg atcgcatacc
    67921 ttgtccgggt gtccttctga aacggattcc gaagtgtaaa tgtgacgtgt gtgcagttta
    67981 ctcatgtttt tttgtaacct ctcttttcta ttttattttt ttaatcaaat atgattgggc
    68041 ctcataaaaa acaaaaagcc cgcgccaaag ggcaacgggc tcattttcat tcacaccagc
    68101 ctttagcaca tttttagagc ggagcaatta acttgattaa atcaccgctt gtcgttaaca
    68161 tactcatcca ttgaaatttg tcaacaatta tttctacttt tttcttttcc cgctgttttt
    68221 ggggaatcgg tataaaaaat catcaatcat tttggtcatg cacacgatcc tgtgttccca
    68281 caaaaaacga aacagacgtt tatagattga tatcacaggg attgttttgt agcaccatga
    68341 aaaggaccgg ctttgttttt ctgcaaaaac aaagccggtg tcataaacca tcttggggtg
    68401 aaagaaatca acatgacgcg gttggccgtt gtcagtgttg atgaattacg tcgtccggct
    68461 atcgtcggtc ctctctgacg ggacgtcggt catcacggta tttgatgacg gtctttgatt
    68521 tttttacttc acgcctgtat tgcgtgccgg agcgccggtc tttccagttt tttacacccc
    68581 agctatgttt ttcccagcgt ttttcttttt tccagccgct ccagttacgt tgaagctcgt
    68641 gatggcttat ttttctgtgt tcccatcggt gtcctttcca ggtcttattg cggtaattgt
    68701 ccttccaccc gggcggaatg gtacgatgga aaaacggcgg ctgatggtca taagtccatc
    68761 ccctgtcgta atagcgtgaa cggtaccagc ggccgttcca cggccgccac caccaaccgg
    68821 aatagaagaa taaatcgtcc tgaatatcgg gtaccgcata gatgtatgtt tccggtatca
    68881 ccaccagatg cggcggcgcc gggaaaatga ttggcggcgg tagagggata tcgatgtgca
    68941 cactgactcc cgccgatact gaaaacggcg caaagagcac gcctgcgaaa aatatgattc
    69001 cggacattaa ttttttcatc ggattacctc ctccgtcggg tcggttaagg ttggtcgaaa
    69061 agtttccatc gtaagtctga tgccccaacg ataaaaaaaa gacaggggct gtcaagcatg
    69121 aagcaacttc ctgccgcccg gttcccgagc catgtttacc ggtcattcga ccattatgat
    69181 ccttgctttg caagcctttt ccgaaacttt aaataatcgt cggcagcgat tttcggttgc
    69241 tctatcatcg gcagatgtcc cagccccttc atgatgatga tttcggagtt ctgcaataat
    69301 ttgtttagaa tcggcgccgt ttggggactg atgacgcgat ctttttctcc ccagacaatc
    69361 agggttggcg ttttgatttc tttgacgtat ctttccgatg catcggacga caactgcttg
    69421 aaaatacgct gttccagctc ataattcctg atgcgttcct gcgccatgac gtttaaaatc
    69481 ggtcgcggaa tgaacggatg atcggtcatg acaaaactga atatttcagc aaactcatct
    69541 tcgtttttgg ccatcagcgg atttttgcct gttttggcga tgatttcatg caattcaccg
    69601 ggcggagcgc tccagattcc gccgggatcg atcaaccaca agctttttac ttcatcagga
    69661 taaagaaaag cgtacatcat tgaaatgtgg ccgcccatgg aacttccgcc cacgtgcaca
    69721 tctttgatcc ccagcgcctg cgccagggtt ctgattctga cagcctgcgc cagtgatccg
    69781 tagtcttcat tttgcggacg gctagaatca ccgaaaccga tgtggtcggg aatgatgacc
    69841 ctgtaatgag gcgtcagaaa cctggccacc cgcgtgaaat tatccttgtt cgcgccgaat
    69901 ccgtgcagca gcatcagcgg ctcacctttt cccccttcca gataaacgta acggatatga
    69961 tccgaaaggt tgatttcctt tttttccagc ccggaacgtt tgcgttccac atccagagcg
    70021 agcgacgtcg cctgttcggg agccagataa acgaatccta tgcccgtcgc cacgcaaaga
    70081 atgatcaata caacaatcgc cttgagtagt cgcattttcc cctcctgttt tttgatcgga
    70141 ttaattctgg ttatctaata tagcaattat tggtcaagat ttaaaatgaa ttcttatccg
    70201 ttttctgcgg atagcgattt ctcttttgcc gtgtttttca gactgccggt ataaagcgcg
    70261 tattcaagca gacggcattc gatatcgccg ttgtacagga tcacacgcct ttttgttctc
    70321 aagccgactt tcttgatcag ttccagattg ccggtaaaaa tatatccatg acaaccagta
    70381 catgtttttt tgaagaaatc gccgatgccc tgataaagcg cttccagttt tttcatctct
    70441 cttccccgat ggcggttgcc gtcgccggac ttgtcgccgg atttacgaag aatgattttg
    70501 cgaccggggc cgggcgtttc tctgctgtga cggaaatccg ctgacaccgg tttgcgagga
    70561 tcggaactca tgcgttcacc gtaaggagga ttaaaaataa tgatgccgtc gcccgcgggg
    70621 acggacgttt tttcgaacgg acaaatattg aattcaatga aatgatccac cccggcagtc
    70681 tgcgcgtttt gtctggctgc cgaaatcgcc tctcggttga tatcagtggc gataattctg
    70741 gccggcaatt ttttcagggc attcgtccgg gctttgctac gcagcgtctg ccagtgatcc
    70801 tcggggaacc ctttgatgaa cataaaaccg aagtgatttc tcagcagccc cggtgcccga
    70861 tgcagcgcaa tggatgcggc ttctatggcc agagttccgc tgccgcacat gggattgata
    70921 aaattacttt ctcccttcca gcctgccgcc ataatgaccg ccgccgcaag cgtctcctgc
    70981 atgggcgcca cgaggggtac cttgcggtat ccgcgcaatg ccagtcgttc tcccgacgta
    71041 tccaggtaaa cagtggcccg gttatctttc cagtatacat gaaccacggc cctgtttttt
    71101 tccggaccgg aatccggccg atttccggaa atgtcgcgta ggcgatccac gatagcgtct
    71161 ttgaccttga cattgacaaa ttgcgaatct ttaatgagcg ggttctcggc gatggaggtc
    71221 acgcagagat acgccgcggg tccggaatca tggagcagat tttcccaggg cagggcatga
    71281 atttttttgt agagccattc cggcgtatca gcggagaagg agtccaattg atataaaacc
    71341 cgctgggcgg tgcgcagatg caggttgagc gccatcgtgt cctgaagggt tccttccgtt
    71401 tgaatggccg tatcccattc tgcccgaacc ggataattca aggcctggac ctcttctttt
    71461 aaaaaaggcg atacgccttt cgggcaagtg accacaatgg tatttttctt attccagatg
    71521 gacatgaact gcttctagca caggtcgttg tttttgataa gcgatatcct gtaacgattt
    71581 ttaacgcgga gggtcaaacc ggaaaaggaa ccgggatgat cacagatatt tatttctaag
    71641 aaaaagattt agtgataaga gagcggccgg gagcgtgatt gccggtgatg caggatttta
    71701 ttgatgaaaa atgatcgcaa aatattctgg ggctggtaca ttgtgggcgg cgcttttctg
    71761 gtcatggcca tgaattacgg cgcgcgatac tgtttcggtg tttttctcaa gccgatggcc
    71821 aatgaattcg ctttatcacg gtccgtcata tccctggcgg cggccatcaa catgatggtt
    71881 tattcattcg gatctgtttt tgtcggcaga atgctggacc gcgttgcgcc gcgctggatc
    71941 acaacggccg gcgccggcgt tgccgcctgc ggttacctgc tgaccggttt tgtcgataca
    72001 ccgatctggc tgtatatcag ctacggcgtc atggtcgggt tggggtccgc cggaatgggc
    72061 gtcgttgcct gcagctcttc cgtcagcaag tggttcatca gcaagcgggg tgtggccgtc
    72121 ggcatcgcat ccatgggaat cagtctggga acggtcatgc tgacgccgct tgcgggttat
    72181 attgcaacag tattcagttg gcggttcgga ttgatggcgt tgagtttcat catgctgatt
    72241 gtgggcgttg ttgttgcgca gacgctgatg agaagaaccc acccggaagc ctacggtctc
    72301 ttgccggacg gcaatacaat atcaggcatg cctcaggcgg cagaggcaac ggccgtttgt
    72361 ccagtgtcca ccatgatcct ttttcgggat tcccgtttct ggacgctggc cgtctgtcag
    72421 ggattggccg ttatgatcag catggccgtt tttgttcatc aggtagcata tgccacagac
    72481 aacggtattt ccaaaatggc ggcagcctcg tctctggcag ccgtcagcct gaccggtttt
    72541 atgggacaat tttttttcgg ctggataacc gacaggatca atgaccccaa atacgcttct
    72601 ttcctcggca tctcctttat gctggcggga acggtcattc tcctgaacgt ggaaagcctg
    72661 ctgcatcttt acgttgcggc tcttgtctac gggttcggat acggatcgct tgcgcccatg
    72721 ctgcctctga ttattgccga ccgattcggc cggcagtgca tgggatcgat ttacggcctg
    72781 ctgacgttct ttatcggcac cggcggcgcc atcggtccgg ttctgggagg ttttatttac
    72841 gaccatttcg gatcgtatca taacctgtgg ctgatgaata ttgtcgtgct gagtcttatt
    72901 gcaatggctt ttctgaccct taaaaagggc aggccctacg ccgcttgatc aaatgaacag
    72961 agggtctagc gggcgagtag aaggattttc ctgatgacgt ctttcgtgta gccttcatag
    73021 caccatactt gcgagagggc gaaagcattc ccggcgattg cgtcaatatc gctttcgggg
    73081 atgtcaactg cctgtaaaga caccggactg ccgatcgatt caaaccagtg cttcaggcag
    73141 gcaattccct tttttgctgc ggatatcgcg ccgcgtccct tcacatgaaa aatttcccgg
    73201 gcaagttttt ccaaccggtc tgtttttttg tccaacgcgt acgacatcca tgccggtaaa
    73261 ataatggaca gtccggcgcc gtgggctata tcataaatgg cactgagcga atgctcgatc
    73321 atatgcgcgg gcattcgcac ctggcccatt cccgccgttg tgagcccgtt aaatgcaaga
    73381 ccggcgctcc acatcatatt ggctcgggcg tcatagttat ccggctcctt caaaatgatt
    73441 tccgtgcttt ccatgacggt ttttatcagc gattcgacca gcctgtcctg cagcgggctg
    73501 gcggaagagg tgttgttgaa atatccttcc agcatgtgcg taatgatatc gacggcgctg
    73561 taagcgctgt aggcggggct gagtgaaaaa agaacggtcg gatccagtac ggagaccttc
    73621 ggttgaataa agggagatac gatgcagtat ttttgacagc cgtcttcccg ggtgacgaca
    73681 gcgccggcat tcatttcgga tgcactggcc gagacggttg ccaccgtcag aacgggaagg
    73741 gcgccggtga cgggcttgct gaacgtaaaa aaatcccaca catcgtgatg tgccacaacc
    73801 cctgcggcaa tagctttcgc agcatcgatc acactgccgc cgccgaccgc cagaataaca
    73861 tcgacgtttt ccccgcgcgc cagtgcaatg cccttttgca catgcgaaag caccgggttg
    73921 gatttgacgc caggaaaatc aacgatttcg acgccggctt tctcaagagc gtttgttacc
    73981 tgatcataaa cgccgttttt cagaatcgta cccatgccga aaaccagcag ggccctgcgt
    74041 ccaaaacggg ctgtttcttt gccgattcgg ggaatctgac tcttgccgaa tataattttg
    74101 gtcggatttt caaaaacaaa attttgcatg attttacccc ttcctacgtg cttttgcggt
    74161 ccggccagat cctatcgccg gcctttgaga cttcttctac aagaaggcgc ccatggttag
    74221 cgcactgatg tccatgctga aatcgagctt tgtgtggacg agttctctgt tgcggaccgc
    74281 aaacggatga agaaccagag aataatgttc cagcgtcaat ttaagcgacg agagcatatc
    74341 tgccagatgt cttttatcag ccattaaact gccaagcgtg acaaggcagc tttcgaatgc
    74401 ttcgctcacg ggcagggtga cctgatgaat attttttacc gggcaatccg gcgttaaatc
    74461 gtcaggcgcg ggcgttacgg ttaattgccg gcaccagcgc agaaacaagg cgggattgag
    74521 cttgaaagcg ggagcaaaaa agtaaaaagg tgttttgtac cagatgtcca ggggggcttt
    74581 cggcagattg gccactttaa tgaagtctgc gtagtttccc agcgcgatac cgtcgacctt
    74641 caccttcagt tgccagaaag ggaggtaagt gatctcgtcc cctttttccc gccacgttgc
    74701 gtacggaatc ggaacaaaat gctttttcga tggattccac gcacgatggc aattctcaca
    74761 aaatacaatc aaggcgtctt tggcgccggg cagatccgcg ccgcattcgg gacaaagcag
    74821 gggcagaaaa tcaacatgcc acttttcgtc cggtgatgaa acgagtttgt cctccatctc
    74881 cggctctttt ttttgtatgt ccagaggctt ctttaaaacg gcatcgtaaa gacggccgtt
    74941 ggcaaaataa accggcgtat agatgatgct ggccgtttcg ccgatgaata tttcctgaac
    75001 ggcgccggcc tgtgttttga aatttttcaa cagttttccc ctggtctttt gagacgctgc
    75061 aatgaatttt ccttcatgat ctgcattgcc cacaaatcgt aatttcatcg cctgaggtct
    75121 gagccccagc gagtggggga aaaaaggcaa atcaaacgag atgaaattgg cgtcaaaata
    75181 tttataagca atattgtgcg ttgtgatggc gtaggagaga cccttgagac gccagtaggg
    75241 gataaaataa attttttcag aaattccggc ggccgggggg atgtaaaaga gaaagtgatc
    75301 atcggtggcc agatagacac gcgtcctgca gaaatggcac gaaagaatac ggtccgtctc
    75361 gtcgagaata atcggcgcac cgcactgggg acattgctgt tcgatctgaa agttagagcc
    75421 gttcaccgca cttattgcaa tacatcgaat ccggcaggtt ttcaaaaccg cacttggcgc
    75481 actgtttgcc gggaggattt acagtgaccg gtttgccgca actgacgcag aaattggcat
    75541 tcacaggcaa gtttcttccg caatgtgcgc atttgttgac aatcagaatc tgatgaccgc
    75601 atgcgggaca gaaatgcgct tccttcggca caacctgtct gcaatccgga caggtgaaaa
    75661 cgtcggcagc aacggttgtt ccggctcctt tgaacgcttc cagcagcatt ccgggcatca
    75721 tgaaacccat gcccataccc aatccggcgc cggcttccga ctggttttgc gctgctgctt
    75781 caacggccat cgccgctttc atttttaaaa gcttgttgag gtcatcaaag acacccaggc
    75841 ggcttttatc gtcgatggct ttttggactt ccggtggcgg ggtgatggaa ttgatgtaca
    75901 ggtcggtcag tgccagaccg aatttgctca gatccttttg cagcatgacc ttcagacctt
    75961 cggaaaactc attgtatttt cccggcagat tgaaaatcgt gtccaccttt tcgcccatat
    76021 aatcgttgaa gcgtgaaaca atgacacggt tcagatattc ttcgatttct tccgtgctga
    76081 aaataccttg tgtacctacc agggagttaa tcagcagaag cggctggatg acacggatgt
    76141 tgaaaacgcc gaaagcacgc aggcggatca gccccagttc ccggtcgcgg aaagccaccg
    76201 ggtcccgtgt gccccatttt aagttgggga ataccttcat gttgatgaag tagacttcgg
    76261 cgcgcagcgg actggtcatt ccccagggaa tggcaatgac tttggtcagc agaggaatgt
    76321 tggcggtttt cagtgtgtgt cgtccgggac cgaaggcgtc ataggccttg cccttgtaga
    76381 aaaagacggc gctctgactt tctcggactg tcaattgggc gccgaatttt atttcgccgg
    76441 agcccttttc cggaatcctg tgtacgattt cttttcccga ttcgtcaaac cattcgatga
    76501 cttccagaaa aattatgttg tttgttccca tgtcaatccg catcctttat tttgtggcga
    76561 ttattgaaat tcaacgtttc agcgtgtaaa aaatcataag ggagggcaac caaatagtca
    76621 agaacaaatg aaggattcaa tttgattttt taaacgggat attatagaga aatcatcatg
    76681 aaaaaaacgt ctgctgagca aaatatatat gacggtcttg cgcttattgc cgatccgatt
    76741 catcagtata ttctttttac cgttccaaag gacaagtctg ccaagtctgc cggagaaata
    76801 acggagcagc atatcattga ttctccctgg atgcagaggc tcagaagaat ttatcagtta
    76861 cagagcgccc gctgggttta tccggcggcg gagcattccc gttttcagca ttccctgggc
    76921 acgatgcaca tggcgggtga atttgccaaa agcatttatc cgagcctgtc ttccgtctgc
    76981 aaagatgttc cgtcctgtca ttacgtggaa gaattactcc ggctggccgg gcttctgcat
    77041 gatgtcggac acggcccttt cggccacttt tttgacgata attttttgag caactggagt
    77101 ctgacccatg aaatcgtggg gcagaacatt atcgtggaca agctcggcga tgatcttgcg
    77161 cgcgtccgtc gaagcccttc cggcggattt tcacgcacag aaagaatgga tgcgcaaaaa
    77221 gtggcgtatt tgatcaggat gcccgaagac ggtgaggagg gcaggccgcc gcgctggctg
    77281 caaatgctcc ggcagttgtt ttcgggtgtt tatacagcgg acaatctcga ttatgtgcag
    77341 cgagacgctt acatgaccgg attctccctg gatatggtgg atatcccccg tttacgctac
    77401 tacacttttt ttacccggga aggtctcacg cttcatcaat cgggcatttc cgcactcaga
    77461 cggttcctca acgcccgtct caatctctac accaacgttt attttcaccg cacgacacgg
    77521 gggctggatc tgcatcttca ggaatttttt catgatacca tgcagatgat tttacccgcc
    77581 aatccgctca acaatcttga cgattatctg caatgcgatg aatggacgct gtttaatcat
    77641 gtgcagaact ggctgcgatc caaagacgaa caaaagaaaa aactggctgc cgaatggaaa
    77701 aaacttcacg accgcgaagt gaaatggaaa atgtcttttg ttgcagaaat ttcactggat
    77761 cagatacagc ccggggttgg cttttcccgg gcagccgatt atgaacaaca gatcagggaa
    77821 cgccttccga aaccgctgaa atcccttcct ttcaaggttg atctggcgac gcaggatccg
    77881 cgtccgctca atccgattac cgagagggaa aagagaatca atatttttaa tcccgtcaca
    77941 cacaagacgt ctcccgaacc gcttctcgaa atttaccgtt ttattccggc acgggtcatg
    78001 caattcagag tttttgctct gaaccatgat catgacggag acctggccgc tgccgctgaa
    78061 aaagttcttg gaagctctgc aaaatcaaca ctgacgaata tctgacatga gtaaaaacga
    78121 cgagacaaca gacctgactc tttttgtgcg gacaacggga gaggaaatcg ttctctggga
    78181 ccgtcagcgt attgtggatg ccctggtgcg cgaagccgat atcgatgatg acgtggcagg
    78241 ccagatttcg aaagaagtgg aaaaacagat cattgtatcg ggaatcggcc tgctcacaac
    78301 ggccctgatc cgtgaactgg ttaatgtccg gctgatcgaa agaggcttgg agaaagagcg
    78361 ccggcttcac ggccgtctgg gattccccct ttatgatgtg cgccagttga tcctgcatcc
    78421 caacaaacag gacgccaata tcccgcattc tcccgaaggc acgaatctga tttttgccga
    78481 aggcattaaa cgggaactct ctttgtttga cgttttttcc gccgatgtcg gcgaagccca
    78541 tgccacggga gatattcata tccacgctct ggggtatatt gaccgcccgt atagctgctg
    78601 ccagtctctg gaatacctca aaatcaacgg gttaaatctg cctcatgcga tcaattccgc
    78661 caatcccgcc aaatacgccg aagtgcttct ggcacatatg gtacgcttca gcgctgtttt
    78721 gcagggtcat tttgccggaa cggtcggctg ggatgcggtc aatatttcat ttgcgccgta
    78781 tctggcctcc atgaccgaca aggaagtcaa acagtttgcc cagatgctca tttatgaatt
    78841 ctcgcagctc gccgcaacgc gcggcggaca ggcgctctac accgacatac acatttatta
    78901 tgatgtcccg ccgcactggg cggaccttcc cgcggtggga gcggaaggaa aaccgacggg
    78961 aaaaacatac ggcgattaca cgtgggacgc ccagcgcttt gccatggcca ttctggaaat
    79021 ttttgccgaa ggggacgccc gaggaaaacc gtttattctg ccccgtcccc ttttgcatat
    79081 tacggatcat gtctggaaaa acgataaggc ccgtgaattt ctgcttcgtg cctgcgatgt
    79141 ggccgcgctg atggggaatc cctgttttgt ttttgacagg gatgaaaaag cggtcgacct
    79201 gattttcaac gacgaggaga ggcgcaagcc ctggcttcag cgccgggccg ctattcagac
    79261 ggtaacgctg aatctgccgc ggctgggtta caaaaccggt ggagacgaaa aagaagtctt
    79321 ttccctgctt gaagccttca tggagaaagc cgttaaagcc catgtgcaga aaaaagattt
    79381 tctggaaaaa ttgctttcct atgccgaaga gggacctctg gccgtactgg ccatgaacca
    79441 cgacggattt ccctttttaa ggatgcatct ggccagctat gttatcggca ttgtcggttt
    79501 gaatgaactg gtgcagattg ttaccggaaa tgaaatgcat caaaaacagg aggcgttgga
    79561 attcggcctg agagttgttg atttcatccg gcaggaagcg cttcgtttaa gcagacaatg
    79621 caaaatgaaa ttcgttttgg ggcagtctct ggcggaaacc accgcgtatc gttttgcccg
    79681 tcttgattta agatatttct ctccgacagc cggacgttat gtcaagggag atattgccga
    79741 gggagccatt tattacacga attcgacgca tttgaatgtt gcagccgatg ttgggcccct
    79801 gcagaaggtg atgcgggagg ggcttttcca tcattacctt gaagacgagg tgattacgca
    79861 tttgcagctg ggagatttga atccgggaag gaacgtcctt gcccggttca tccgcgacgt
    79921 attctatcat tccgcaaacc gccagataga tttcaatccg gaattcacgt tttgtctttc
    79981 ctgcaaggaa acgaccagag ggttgcagga caaatgtgcc gcctgcggtt ctgccgatgt
    80041 ggagggcatc gcgaggctca cgaagtattt cagcaaaacc tccggctgga acaaggggaa
    80101 actcgccgag ctgaaagaca gaacaatgaa tgacaacttt aataatgtcg atcagccgaa
    80161 atgttaagac gaagctggat tgtccgcggc atctcgacag ttttttctta atacaaaaag
    80221 catgtatttc ttttcatttc taaattttat cgggtcaacg gccgccagtt ccttccatat
    80281 tttatgatca acggattgct ttaaatctct gatgaggtag tataaccatg cctttttacg
    80341 cttgattttt ccggatgtaa aatggaccag gggtaaaaga aatcgttcag cataattata
    80401 gacaaatttc agcgtcggca aaacatgagg cgtcatgtcc tgctgatacg taatctccca
    80461 gccatgttcc tttgatttcc gaacaaaatc atcgagcagg tgtgatgact tgttgacgcc
    80521 tgaattatcg aatttgaaat agtccgcaat aatccatgtg ccgtcaggtt tgacaatgct
    80581 ttccgctttt tcgaacgctt tctcaagcgg tatatattga agcgattccg aattgatgat
    80641 ggttccatac tttttatcgg tttcgaattc ttgaaatcgc ttctgataac acgtaatcgt
    80701 cgggtacttt gattggatat gcctgatctg atttttattc ggagtcaacg cctcaacatt
    80761 aaatcccttc tgggatatta agttcgacaa gccacccatt ccgcagccga tgtcaagaat
    80821 taaatcttgc gggttgatga tttgatcgat gatgttctgc gcatatctga cctgggcttt
    80881 ttcaatttca gcgatagaga tggtgtcgac ttcaatctcg gtattttcga aatacccata
    80941 atgaagcatg tcatttttga atattttcga ataaagatga agttccaggt cggaagttga
    81001 gttgctgaag gttgaatttt tcttgtggta aagccaggcg ttaaaaagaa caacaggatt
    81061 tataattttt aaaagtattt tcttcattat ttgcgggagg gactatatgg gaaacattat
    81121 tctatgtcaa tgacaaacta accgacgaaa gtcggcgatt gaattgatat tgattgaatt
    81181 gattattgat tgataattat tgattgattg aattaattga taagaacttg cttttagttg
    81241 acggcagtat aagaaacgca ctcttgtg
//
LOCUS       DAOSME010000003        70442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_490_length_70442_cov_20.241053, whole genome shotgun sequence.
ACCESSION   DAOSME010000003 DAOSME010000000
VERSION     DAOSME010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 70442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70442
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_490_length_70442_cov_20.241053"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1686)
                     /locus_tag="PLG98_00735"
     CDS             complement(100..1686)
                     /locus_tag="PLG98_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE31803.1"
                     /translation="MTHLKLVEAMQKPDFYPHRPANVEFIQTHISYVFIAGDYVYKVK
                     KPLKFDFLDFTTREKRKFFCEEELKLNRRLAPDTYLDVVSLSRNEAGDITLDQGIEIV
                     DYAVRMKKLPGDRMLKTLLARGTADEKIMDALARKIVLFHQQARTDSRIDKMGDIENI
                     RHNHEENFAETEKFIDVTISEYQYSLIKDFANKFLIENKKHFEQRITDHKIRDCHGDL
                     HLDHICINDEITIFDCIEFNERFRWGDVAEDVAFLTMDIDFNGWPHHAENFIQSYLKY
                     SGDTGLLKLLNFYRCYYAYVRGKVTSLRLLQEDTTADEQRDITETAEHYFDLAYTYAA
                     RLDKPVLILTAGLMGSGKSYQARRLAVRLGTEIIRTDELRKALFNIRTTERRHERFGD
                     GIYSDDISRLVYEKALALAEQKINLGKAVIIDASFKKRTERLKALALAKKLQIRFFVL
                     ECFCPDDITQKRLEKRVLDNDNVSDGRWELFQQQKDNFEPIDEITADCRFKIDTSEKP
                     EVFRHEMIRKIKLAEFEMVK"
     gene            complement(2452..3102)
                     /locus_tag="PLG98_00740"
     CDS             complement(2452..3102)
                     /locus_tag="PLG98_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOE31804.1"
                     /translation="MSQMDNIQGLITSAETDLLQEIMNIAFGRAASDLAEFIDIFVVL
                     SVPQIKLLPAYQLPDYINSEIMDYDQDRVSVVEQSFWGKFKGNALLVFPSGTGKRMIS
                     LFDGKDEFFQSDPSLEQEKETFLEIGNILIGACIGKLAELLGDIATYSPPRVLVEKSL
                     HRVYESIINPDSMAIVLRTVFSFNEKNISGFMFVLTKQESFAWLKNALHKFLEQYE"
     gene            complement(3106..3486)
                     /locus_tag="PLG98_00745"
     CDS             complement(3106..3486)
                     /locus_tag="PLG98_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE31805.1"
                     /translation="MIKKILIVDDSPISVKIIKSCVPKDMGYELYDAADGQMGVEKYK
                     EIKPDLTFMDLTMPVMTGFQALEEIMKMDTKAMVIILTADVQIKAIAKAHDLGAFSVL
                     KKPPSKESVAGAIKEAEEALASRG"
     gene            3904..5973
                     /locus_tag="PLG98_00750"
     CDS             3904..5973
                     /locus_tag="PLG98_00750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOE31806.1"
                     /translation="MNLNRLAISLIELNPENPADLEEFKKNLLSCYNNTQGRIQNILK
                     EACFEADAMVDQVPELQRNIIARLGVLLEQAMGSDETDISATTEQTSQVEPAREPDSI
                     DVQHVMLSADLDADLLNEFIIENMEWATMAESAILDWEQNPGNQELLNTIFRGFHTIK
                     GTSAFVNLDCVKDLAHQVESVLAKVRDGEHVFTRSYANLALKSLDVIKSILEQMKSSG
                     PGQQIELPRGYQGLLESLRNFVPDEQVQEMAIPAEESMVEPEAAAPAYTKTNRGKIAK
                     KEGEASSEQSASSNKIREQMTESTVRVKVDRLDKLLDTVGELVIAQTMVGQDNLIVSG
                     KYHELNKKISQAGKIVRELHDLSMFLRMVPLKGTFQKMVRLTRDLSLKHGKMVSCITE
                     GDDIEIDRNMVDMVADPLIHLIRNAVDHGIEFPEERRRAGKSENGRIYLTAGHAEGSI
                     MLTVWDDGRGMNRQTIVNKAIQKGLIDSAERMTDQEVWQMIFLPGFSTAEKVTEVSGR
                     GVGMDVVKQAVEALRGRIEVQTTEGQGCTFIMRMPLTMAITDGMIVKVGSQRYILPTA
                     NIHKAVRPQEPDIHTVNQRAEMLSFRDEMVPVIRLYRFFDIPDSLTDLTSGLLVVIGE
                     GKQRCALFVDELLGQTQVVTKLLGKGMNNVPGIAGGAIMGDGTVGLILDASGIVSIAR
                     QQTNTTT"
     gene            6001..6534
                     /locus_tag="PLG98_00755"
     CDS             6001..6534
                     /locus_tag="PLG98_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOE31807.1"
                     /translation="MNNANAVKNDQSANADSRAGKYLTFFLASEEYGVEILKVQEIIG
                     RMPITPVPLTSRYIRGVINLRGKIHPIMDLKIKFGMEETQITDETCIIVIKTTSMMMG
                     ILVDKVSEVINIASGDIEDTPSFGGDVNPEYLLGVGKTGGRVRLLLDIEKVITASDII
                     NMKKAAAGEGKDAMAEL"
     gene            6589..8574
                     /locus_tag="PLG98_00760"
     CDS             6589..8574
                     /locus_tag="PLG98_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE31808.1"
                     /translation="MLNDIKVGVKLIGSIVTVCLIAAAVILYGIKVRNDVVHKVDSVH
                     RQYEINIRDIGNVRVSLEKMGGDIYRYFGAPADRQKMSKEINETISSVDGTMQSYKNR
                     SIDVDEKKLIADFESAWSEMQRRYKEGMKAVDEGRQNDINQFIGAGSAAMQAREKTSA
                     TLRDLNNYSVNKNESAVKATIDNVGGLSSIMLILSAIAIALAIVTIVFLTESITKPLK
                     KGVQMMAEMKKGHLSNRLDIKRRDEIGLLANAMDEFADHLQKNIIFNMQQISEGNINV
                     APVVIDEKDEIGPALKKIIETINSLLNEVDMLTKSATEGKLDVRGNEQDYKGAYQGIV
                     QGVNRTLDAVIDPINEASAVLERIADKDMAARMVGDYKGGHAKIKKSLNLAVENLDRA
                     LQQVAIGADQVASASVQVSTGGQSLSQGASEQASSLEEISSSLQEMSSMTKQNTLNAR
                     EAKGVAEGARESADKGVESMNRMSSAINKIKTSSDATAKIVKTIDEIAFQTNLLALNA
                     AVEAARAGDAGRGFAVVAEEVRNLAMRSAEAAKNTASLIEEAVRNSENGVTINTEVLT
                     NFQEITEKINKVSQVVAEIAAASEQQDQGINQVNKAVEQMNQLTQQNAANAEESASAA
                     EEMSSQSEEMRAMVAGFKLTGSGYTERESIAYEPKDF"
     gene            8685..10787
                     /locus_tag="PLG98_00765"
     CDS             8685..10787
                     /locus_tag="PLG98_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE31809.1"
                     /translation="MINNMKMGVKLAGVFAIIFVVVTAMMLSGAAVRMKALRMLDSNY
                     RDYVNDIKNIGTIEVGLVNMEKDLHLYVNSPAARENALIQIQEGITSTENSFQSLKSS
                     KRDSEEQKYLSEFEVAWPEIQRGYKAVIKAVDEGKNDDANSILADKSYLNEAGRKALA
                     VVKNLNQYSVNKNETRVKQYVKGRGGWSGILWIFSGFGALALIGLGTMVHRNITRPIK
                     TIVLMMQEIEKGHLSKRLKMNRKDEIGSLSRSMDMFADNLQKNIVYSMQQISEGNIDV
                     EPKVTDSKDEIGPALIKMINAIGSMSNEVKRCCLNALEGNLQNRADVTPYQGSFRQII
                     QAFNDTLDAVMNPINEAALVLTKVADKDMTARMAGDYKGGHAKIKESVNMVVDNLDGA
                     LQQVAISADQVASASVQVSTGGQSLSQGASEQASSLEEISSSLQEMSSMTKQNTLNAR
                     EAKGVAEGARESADKGVESMNRMSSAINKIKTSSDATAKIVKTIDEIAFQTNLLALNA
                     AVEAARAGDAGRGFAVVAEEVRNLAMRSAEAAKNTASLIEEAVRNSENGVTINTEVLT
                     NFQEITEKINKVSQVVAEIAAASEQQDQGINQVNKAVEQMNQLTQQNAANAEESASAA
                     EEMSSQSEEMRAMVAGFRLTKTVALTAGPDAIKKTEYRLPDVAPGKKGDKASAGLHPD
                     PRKVIPLDDKDHDILQNF"
     gene            10856..11755
                     /locus_tag="PLG98_00770"
     CDS             10856..11755
                     /locus_tag="PLG98_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOE31810.1"
                     /translation="MHSEWNNLSTDYYSLKLTSEQFDKISRLVYQISGIDLHEGKEEL
                     VKARLIKRLRHLKFHDFNQYLKHIANDKSGTEIGAMVDILTTNKTNFFRESEHLDFLK
                     HEIIPGWVKGPVRIWSAGCSSGEEPYTIAIVLLESIPDTATLDIKILATDISDRMMEK
                     ARQGLYDEESLAIMSRHLKHKYFQKTDTGSGYKYKVIPKIQSMVSFAKLNLMEHWPMK
                     GPFDVIFCRNVMIYFDKSTQEKLVGRFWHLLKEDGYLMVGHSESLSFLQHEYRYMRPA
                     VYQKIRKAHSVVKKTGKVRTDVK"
     gene            11757..12239
                     /locus_tag="PLG98_00775"
     CDS             11757..12239
                     /locus_tag="PLG98_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOE31811.1"
                     /translation="MVYTVGVADLKISDESADMLITYALGSCLGVTVYDFRKKTGGLL
                     HCMMPDSGIDQNKAAGNPYLYVDSGMKAMMDKFYQKGSRKSDLIIRVAGGSSSKLNEN
                     EDFFQIGRRNFVSLRKYLWNEGLMLKAYDVGGYGSRTVTLELESGKMLIKSQTFLKQL
                     "
     gene            12295..12687
                     /locus_tag="PLG98_00780"
     CDS             12295..12687
                     /locus_tag="PLG98_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE31812.1"
                     /translation="MALNTLVVDDSSVMRTIIIKTLKLAGLPLGDIWEAKNGEEGLHV
                     LQDHWVDLAMIDIHMPVMDGEEMLIKVRENDQYKNLPVIVISSESDPGKIEKMLELGA
                     TFIHKPFTPEVLKEVIVEVTGVSHENED"
     gene            12671..13150
                     /locus_tag="PLG98_00785"
     CDS             12671..13150
                     /locus_tag="PLG98_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOE31813.1"
                     /translation="MKTKIEKILEEATIKTFEDICFMYLEPELKDSQAALEPDAAAEV
                     EFHGAYNGRLVIASRGGLFSAIASNILSSDHPSLQEKKDALGEIANIICGNIVPSLGR
                     RKAGGYKIQSPRHLGRDELLKEEKSKPSAVVILNFNQGRADIKLFVDDYISDQEIND"
     gene            13147..14199
                     /locus_tag="PLG98_00790"
     CDS             13147..14199
                     /locus_tag="PLG98_00790"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOE31814.1"
                     /translation="MIKVLVVDDSALVRKVLTEELSRHKDIEVVGGAIDPYVARDKIV
                     RLKPDVITLDIEMPRMDGLSFLSKLMKFYPMPVVVVSSLTPRDSQNAIRALELGAVEV
                     ICKPGSSLSTADLSVRLVEAIRAAARAKIAKPAVELVERESVPGYKIQLKTTHKVIAI
                     GASTGGTIAIEKILSRLPMNIPGIVVVQHMPAYFTASFAERLNALCKIEVREAKDGDT
                     AAPGLALIAPGGYHMLLERSGAFYQVRIKGGPMVHHQKPSVDVLFHSVAKHAGANAMG
                     VLLTGMGADGADGLLAMRQNKAYTIAQDEATSVVYGMPGEAVKLGAACDELPLDEIPG
                     RIITRLSGEDKKREDK"
     gene            complement(14204..15283)
                     /gene="rdgC"
                     /locus_tag="PLG98_00795"
     CDS             complement(14204..15283)
                     /gene="rdgC"
                     /locus_tag="PLG98_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOE31815.1"
                     /translation="MGLLKGGFTFSRFSVDGQLPQAFLNFVNNRIRANSFKNSFKSTE
                     EKTMGWVSLTDILDTDFKNANYSLGAYLIFSLRIDRKLIPPKLLKIKLMEEERRFLAE
                     SGKNKIFRQQSTALKDKVKLDLLAKLDAIPFFYDVCWAVEQNTLYFSSLSDKVIDDFA
                     DLFKKTFALNLKRFSPQENHLIRKDADPAESTSLIGREFLTWLWFKSEERNGRIVLPN
                     KEECEIHFLKRVSLEAGEGEYSQGVVCHGIHAELKEGKEAIRQGKKVKEAGIKLLHNN
                     NEWDFMFKADSFDFQSLKMPQTDWQDTPEDPSGSLLERIYLIENAVKTIDTIYESFLH
                     IRQSPQWKEKELKLLHKWLEKNPKD"
     gene            15460..16350
                     /locus_tag="PLG98_00800"
     CDS             15460..16350
                     /locus_tag="PLG98_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOE31816.1"
                     /translation="MKIKKIGIAANIEKEKIADHVRSLKKWLEERGAKVFLETEISGK
                     ILSDGGYSWSELARKAEVAVVLGGDGTILRSARYLAVHRVPILGINMGGLGYLTEVNL
                     NEIHSTMELILKGKFVTEKRMMLDVSVQHGKTVAGIGSVLNDVVMNRGDLSRMNELEV
                     EVDGEYLTTYKGDGLIVSTPTGSTAYSLSAGGPIVFPGKDLILINPICPHTLTNRPII
                     FSEDAHLRITLWSKDKGAMLSLDGQETHKIKSGDVVIVKKSRHATMLILSPYRSYGEI
                     LRSKLGWGDLPSGAHKRKNA"
     gene            16343..18055
                     /gene="recN"
                     /locus_tag="PLG98_00805"
     CDS             16343..18055
                     /gene="recN"
                     /locus_tag="PLG98_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOE31817.1"
                     /translation="MLNELSIKNFAIIDELHVTFGEGLNVLSGETGAGKSIIIGAVSL
                     LLGDRATSEMIRTQADTATVEAVFDISADKPLRDKIDAMGFPVSDELIIRRVISHTGK
                     NKVFINGQAATLANLAAISERLINICGQHEHQIILAAENHIDILDEFGGCLSERAVYT
                     EHFNQYQQIRSGIEKLEELSKSADARTELYEFQLKEIQDINPQPNEDTVLSDEKKVLV
                     NIQRLSEWAGNAFDLLYGDKNSVIVMLKEVQRQIKEIKKIDAGLKISEAEMENNFVAL
                     QEAALLLRDYGKGLFFDAERLAAIEERLDLIHRLKRKYGGSLEAVFQKKQDMEEALRK
                     VSSLDEELQKMIREEEAVKSRLRQSARNLSESRKKAAKTLEDKVGKEIRALNMPHASF
                     FVHFKNRADDNTDFFGPRGADDIEFDLSANKGEDARPLNKVASGGELSRIILALKNVL
                     SGTGSVLSMVFDEVDNGIGGAVAEIVGRKLRAVSANHQVICITHLPQIACFGDKHMFV
                     SKKVVKGRTLTFVNELDSEQKIEEISRMLGGVDVTPTTREHAREMLQNAGSHNLKVSG
                     GDTC"
     gene            18049..18507
                     /locus_tag="PLG98_00810"
     CDS             18049..18507
                     /locus_tag="PLG98_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOE31818.1"
                     /translation="MLRKARIDDVKIIHRLINLSSGKGEMLPRSLMDIYGSLRDFFVY
                     YDEDESRIIGICAMNIIWANLAEIRSLYVEESHRGKGVGRVLVEACISEAITLGLFKV
                     FTLTYKKDFFLKLGFKEIDRNLLPEKIWSDCFRCSKYPDYCDEVAMIVEL"
     gene            18504..19661
                     /locus_tag="PLG98_00815"
     CDS             18504..19661
                     /locus_tag="PLG98_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOE31819.1"
                     /translation="MRYSLKILLLILMAFFLGGCMRAVSQPSFKTLDSLIPVDATQVA
                     FSDDLEPASLETAIDRSIRFYEGRGRNNVYQIEDRQIDAQQFKETLLAFRALLGDAKD
                     PETLGKRMAEAFDVYQVTGTDDESRVLFTGYYEPLLEGSLQKTEKYKYPLYRVPPDLV
                     RKGTRIGRMQNDKFVPYYSRREIDVDGVLRGKNLELLWVSDPVDLFSLHIQGSGKIKL
                     ENGDLLTVGFANTNGRPFRSITKFLLDKGTIQHHEVTYRHDFLRGKRDEEIYEALSHN
                     ERYTFFRFLEKDPVGSLGETVTPDRTIATDPEYFPEGALAFIRLRKPVFNTDGHVKER
                     VEFSRFVLSQDKGAAIKGPGRVDLFCGFGEKAEKTAGTLKERGELYLLLKK"
     gene            complement(19777..21003)
                     /locus_tag="PLG98_00820"
     CDS             complement(19777..21003)
                     /locus_tag="PLG98_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE31820.1"
                     /translation="MTNVKKKVKAFYNPPSLLLKQLGSSLLMAYLNNWRNYPRWGFQY
                     GFRSIPVANGALGMGCIGYPGHPVWEVTGACNLKCIHCHAVSDKAGKDELSTDEGKRL
                     IDSIASVSAFRTLVYTGGEPLVRPDIFELLRHSQQAGLANIIATNGTLIDEEMAFKLR
                     DHGVVCHAISLDAATPAIHDMVRNKPGAFDRTLRAIKATKKAGILLQINTTAMEYNMP
                     HLPELIDFADRCGAGIMLMYQLVAVGRGEKIEKATLKKSANKHLSEMIAEKQKNATTI
                     IEPVAGPQYWPYLLEKKGFTEGLRLKLAGKVFHGCAAGRGFVYVKANGDVWPCPFVEV
                     NAGNIRDRNFKAIYEESPVFQNLRRREELLKGACGECHYKTVCGGCRGRAHAYSGDYL
                     QEDPRCYIRETTQNHS"
     gene            complement(21009..21929)
                     /locus_tag="PLG98_00825"
     CDS             complement(21009..21929)
                     /locus_tag="PLG98_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOE31821.1"
                     /translation="MQSVHFEKIKSWFSLSRPAFHTVGILPFILGTILAYKINLVFDP
                     AVFALGVSAVILIMLSTYHMGEYFDYREDEISQRLYKSRFAGGSGVVPAKTVKRSVAL
                     RTGLISFIIAGIIGLTLQFILKTGPYTLLLGCLGAFPGFFYSTQPVRLVQKGVGEIFI
                     GFCYGWLPVASAYYIQTGFVHSVIHWITVPIGLTIFNVIFLNEYPDYEADKATGKKNL
                     LYRLGKRKGKVLFFVFSLLASTAVFSSPVFGVPFKAVYLYLPVAIVSLFIAVMMLRDQ
                     YENRKTMELLCGLNIAVNLGTSLVYILAYI"
     gene            22340..22612
                     /locus_tag="PLG98_00830"
     CDS             22340..22612
                     /locus_tag="PLG98_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE31822.1"
                     /translation="MTKAELIGVIAKSAGITKDKAGKALDTFQDAVTKELKKSGKLAL
                     VGFGTFSVVKRKAREGRNPQTGKTIKIPAKKVVKFKAGKELAGKVK"
     gene            22895..23884
                     /gene="trpS"
                     /locus_tag="PLG98_00835"
     CDS             22895..23884
                     /gene="trpS"
                     /locus_tag="PLG98_00835"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOE31823.1"
                     /translation="MERKRILSGMRPTGKLHLGNLHGALRNWIALQESGQYDCFYFVA
                     DWHAITSEYSSTEGIQDNVINMVIDWLSVGLDPQKSTLFLQSAVKEHAELFLLLSMIT
                     PLAWLERNPTYKEMKTELANKDLSTFGFLGYPVLQAADIIMYKAYGVPVGVDQLPHVE
                     LTREIARRFNFLYREIFPVPEPILTNVPKLLGTDGRKMSKSYDNSIYISDRGKNLQHK
                     ISSMFTDPQRMRKKDPGNPDICNVFTFHGLYSSPEKVKEIDVECRRAGIGCTDCKKML
                     AQRVAENLQPVHERMDYYVSHMDEINGVIEEGNRKAAKIARQTMSEVRAAMKI"
     gene            23894..24670
                     /locus_tag="PLG98_00840"
     CDS             23894..24670
                     /locus_tag="PLG98_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOE31824.1"
                     /translation="MDNNNLGHPMDPMDNEQRLNYAIKLDIFEGPLDLLLYLIKKNEI
                     DIYNIPIALITEQYFQYLKMIKALNLDLAGEYLVMASTLIHIKSRTLLPPPEEPEEEE
                     EDPRAELVRQLLEHKTFKEIAVSLGNRPLLERDVFTRAAALPEVEVKSTDEEEEFIEV
                     SVFELIEAFHRIISQMDKKELMEIDLEKLSLTDIINEIMEQLTVTKNITFEDLLKGKK
                     DRRRIIYTFLAILELIKLRMIKAYQTSMFGVIRIFPAVES"
     gene            24671..25336
                     /gene="scpB"
                     /locus_tag="PLG98_00845"
     CDS             24671..25336
                     /gene="scpB"
                     /locus_tag="PLG98_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOE31825.1"
                     /translation="MEKIKAVIEALIFASDTPLSPERIRAVFPEVEKTEIREIIDQLV
                     SEYHDRQGGILLQEVAGGFQFRTNPDLSLWIKKLKTAKPQSLSPQALETLAIIAYKQP
                     IVKSEIEDIRGVDVGGPLKGLLEKKLVRIVGRKDVPGKPIIYGTTRKFLEVFNLKDIM
                     DLPNIRELKELHQQQELLDPDQESSESEQETIVEEEAVAKQHDDENAEENAEEKIEEV
                     PDL"
     gene            25333..26061
                     /locus_tag="PLG98_00850"
     CDS             25333..26061
                     /locus_tag="PLG98_00850"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE31826.1"
                     /translation="MKERLQKVIAAAGITSRRHAEELITRGRVSVNGVVVTKLGEKAD
                     AQKDIIRVDGKIISTEKVKYYIVLNKPAGFVTTLHDPQNRPTVVDLLGSVPERVYPVG
                     RLDYDSKGLLLLTNDGDFAQKIQHPRFQKPKIYKVKIQGHLSRQDVQQLSRGINVDDD
                     FFKPENLKVDKMNDRSCWLRLTLKEGKNRIIRRGFEKIGYRVALLVREAIGDLALGTL
                     KEGEWRHLTRKEISQLLDSAVKSK"
     gene            26173..26454
                     /locus_tag="PLG98_00855"
     CDS             26173..26454
                     /locus_tag="PLG98_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE31827.1"
                     /translation="MNKSDLIEALSTKLNLTEKKAIDVVNLIFKGFTDELKKNGRIEI
                     RGFGSFVVKKYGSYTGRNPKTGSKIQVEPKKLPFFKVGKELKVRVDKKK"
     gene            complement(26539..27129)
                     /locus_tag="PLG98_00860"
     CDS             complement(26539..27129)
                     /locus_tag="PLG98_00860"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOE31828.1"
                     /translation="MAITLPELPYAKDALAPVISANTLDFHYGKHHKAYVDNLNKLIA
                     GTDLDQKSLEEIINIAAKDSAKAGIFNNAAQVWNHTFYWKCLKKTGGGRPSGAIAAKI
                     NEMWGNYDKFVEELKNAGATQFGSGWVWLVSDKTRLQITKTANADTPLAHGQKALLTI
                     DVWEHAYYLDYQNRRPDYLSAVIQNLINWDFVNANL"
     gene            complement(27304..28230)
                     /locus_tag="PLG98_00865"
     CDS             complement(27304..28230)
                     /locus_tag="PLG98_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31829.1"
                     /translation="MTLPFQAYRSSIIFIIALFCGMVFDEGRQAGRILILPALMIILT
                     ITLLRFPRGFFRHPASLLFSSIRGNLLNYLVLGNFILLTGAFLIQKQELWIGLALVAA
                     MPASLEIILLGILLRIEKNYVFTSISGTYLGALLIVPLVSLCFFKYIQINYWNIAVLI
                     LCLIALPLVLSRLIVEKDWDRILRKHENTLIDFCHFIVFYAISANSSMFLKKLSDDLV
                     FISLIALTSTFLFYFLIRRIGLYFHGQENKVNFFILLGTMKDYGLAGGIALTLFNPEV
                     AVPPLVFAVFTFIYMNWLSFKARRIEDDCGDV"
     gene            complement(28237..29064)
                     /locus_tag="PLG98_00870"
     CDS             complement(28237..29064)
                     /locus_tag="PLG98_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOE31830.1"
                     /translation="MELSGIFQYAFVQRAFLGGILIAAIASILGVFLVLRRFSLIGDG
                     LSHVTFGSVAVVMLLGISPAHMTLAALPLVMLSSLAILKLTGSKRIQGDAAIGIISSF
                     GIAAGIILTSISGGYNLDLFSYLFGNILTVTLTEIFFAIIVFLTVVATVIILYDDLLA
                     ITFDEELARTMGVQTGKINTVLFLLTAVAAVLAMKVAGIMLVSAMLIIPPLTALQLSL
                     SFRMTIFTSIIFSVLSVILGITMAFLLNLPAGGTIVVLNIFFLLCVFFARKLMAFGR"
     gene            complement(29055..29804)
                     /locus_tag="PLG98_00875"
     CDS             complement(29055..29804)
                     /locus_tag="PLG98_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOE31831.1"
                     /translation="MSGEIIKVDHLSFCYNGTKVINDISFTVQRGDYLGIAGSNGSGK
                     STLIKNILGILEPHQGHITIFDQPVASFDQWHRIGYLPQRINALHYHFPCTVAEIVQL
                     GIKKRNPDDLKRTLDLMGIAHLSSCLIGELSYGEQQRVMLARALIRKPDLLIFDEPTT
                     ALDPETRDVFYALTAKANEHGTTVVLVTHDTGTIGKYARNLLYLDKKIIFFGTFRDFC
                     ASPDMTGFFGPNSQHMICHQHDQKGPATPWN"
     gene            complement(29789..30733)
                     /locus_tag="PLG98_00880"
     CDS             complement(29789..30733)
                     /locus_tag="PLG98_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOE31832.1"
                     /translation="MMLLKRTPLLILLCLVLIFSCQQAKDPGRNDKKLNVIATIFPVY
                     DFARHIGGDKITLAMLLPPGTDAHHYELKPDDIIKVTNADVFLFTNFELEQWAYKIIG
                     ASDKNTRLQAVETGNGAMMLRLNKEEDDHEAHPAGFDPHIWLDMDNAQKMVDNITSAF
                     IKKDPRNSDYYLKNAGDYKRQLADMDQKYRTTLAACKTRTVLHAGHWAFAYLTSRYHL
                     KYIAAYNVFADAEPAPQKIFALIDQIKNENVSHVYYEDMMNPRLAKTIARETGTGLLK
                     LNNGHDVTRTDLEKGETFLALMEKNLVNFQKGLQCPEK"
     gene            31002..31538
                     /locus_tag="PLG98_00885"
     CDS             31002..31538
                     /locus_tag="PLG98_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOE31833.1"
                     /translation="MKPIGPLMWEHRLIEKMLTAMMRHMDQVEKTRKVNPLVIDMAVD
                     FIRVYADRTHHGKEEEILFRDLAKKDLPADLKNIMQELLDEHVWGRKTTAALVEAKNK
                     YLQGNQAELFRLIDTARQLGNFYPKHIEKEDKHFFYPCQEYFNKEEQTKMLDEFWEFD
                     RKMIHEKYNKIYDDYSGL"
     gene            complement(31542..32195)
                     /locus_tag="PLG98_00890"
     CDS             complement(31542..32195)
                     /locus_tag="PLG98_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31834.1"
                     /translation="MTEATQQALTGLRDLSMIKWYVIPLLAIVFYIYTKEIKDARQNG
                     DWNAVLAGLTIFGVDFFNETWNGWVMYLTQRSAFWTTPGDTALRTLVGWNIEIMFMFL
                     IIGIIFYHTLSESKKDKILGLPEKWFWAISYSIFCVFVECLLNIGGHLVWEYPFWYLS
                     FKGVWLIFLIGYFHFFCFAILVISLKSIKAKLTTVGIIYAAPIIMNVLAFGFWGWNY"
     gene            32360..33046
                     /locus_tag="PLG98_00895"
     CDS             32360..33046
                     /locus_tag="PLG98_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31835.1"
                     /translation="MNLKPIYRLFAVVMIFSVFCLSGCAGPRLYSVGMYYDAEQSVIP
                     AYVKNDGQTSDAVVAVSEFADTRQIDDPLVIGRVIQNSGEKTLVFPRNVRATRAVANG
                     IKQYLKKAGYKITEINEPWNLKEDKMPMGKAKILIGGNIEELEIICRKGKLTNSYDTT
                     MTLAVVIADMGQGRILYKTRVKSSYSREHILFSENILGQQADKVLSDTIEKLFEDQKV
                     AQTIKDVMVR"
     gene            complement(33053..34093)
                     /locus_tag="PLG98_00900"
     CDS             complement(33053..34093)
                     /locus_tag="PLG98_00900"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOE31836.1"
                     /translation="MIIKNMKVAYWYNNQDIRIEDIPTPKPGAKEMLVKVMSCGICGS
                     DIVEWYRLPRAPLIQGHEIGAQVVSTGKSITKYKPGDRVFIAPKVPCMTCHYCRNGHY
                     PQCAEIKERLPGGFAEYILVPEILIKNGTYLLPDSIDYDQSTFIEPLACVVRAQRLAR
                     VKKGQTVLVIGCGMSGLLNVKLAVTKGCRIIASDINRKKLDIALEMGAHIAINAADDI
                     SKRLVDQTGRKADIVFLCASADAAVEQAWQCVDKGGVIVLFAVPAPDKKVVVPVNDFW
                     MKEIAILTSYYCGPSDIIEAMKLIEWHHVAIDDLITHRLPLTEIVKGFQLVADGRESI
                     KVIIKPQEQAAL"
     gene            complement(34242..35117)
                     /gene="lsrF"
                     /locus_tag="PLG98_00905"
     CDS             complement(34242..35117)
                     /gene="lsrF"
                     /locus_tag="PLG98_00905"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOE31837.1"
                     /translation="MPDADGIKEAKMFHCDMPQETQGFFLKGSSSLDWGMKNRLSRIF
                     NPKSGRTVMLAIDHGYFQGPTTGLERVDINILPLAPYADTLMLTRGILRSIIPPSLTQ
                     AVVLRVSGGASILKELSNEEIAVDIEDSIRLNVCAMAVQVFIGGEFERQSIINMTKMV
                     DIGNRYGIPTLAVTAVGKDMARDAKYFRLACRICAELGAHYIKTYYVEKDFETVTSSC
                     PVPIVMAGGKKIPELEALTMAYNAVQKGAAGVDMGRNIFQSDAPIAMIQAVRAVVHDN
                     ETPKKAFDLYKSLKR"
     gene            complement(35311..35604)
                     /locus_tag="PLG98_00910"
     CDS             complement(35311..35604)
                     /locus_tag="PLG98_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31838.1"
                     /translation="MKMFYVIYAEAADDDIIAAFKKTGFRAYTKISGLMGEGEETEPK
                     LGTHYWPGNNNALMLAVSDDKIKPLCELVRNLKVEHPRAGLRAFTFPLEEECV"
     gene            complement(35646..38753)
                     /locus_tag="PLG98_00915"
     CDS             complement(35646..38753)
                     /locus_tag="PLG98_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE31839.1"
                     /translation="MWLADTSVKRPVFATMVIMALVVLGIVSYPSIGVDLFPKVDFPI
                     VTITTTLKGASPDVMDVDVADRIEGAVNTINGVKTITSTSTESVSRTIVEFVLERDID
                     QAVQDVREKVSTIRSKLPDDIEEPRIAKVDPDSQPILYMNLTGEKSIRDLSTYVDEVL
                     KEQLQRINEVGDVTFNGLRLRQVRIWLDAAKMRAYEVSPSDVVVALKRENVELPGGRI
                     ETQTKEYTVRIKGEFPSIPDFNDLIVSYYKGAPVKIKDIGRAEDGTEEKRSLARFNGI
                     PAVGIGIQKQSGTNTVAVAARVKKEVERINQTLPPGMKINIAMDQSVFIVRSIDEVQK
                     HLILGSLFAVLAVFLFLKNIRTTLISAVALPVSVISTFALINAFGFTFNNMTMLALSL
                     SVGLLIDDAIIVIENIYRHVEEGMGPVEAAKFATSEIGLAVMATTLAIVAIFLPVAFM
                     KGLIGRFFLQFALTIVFAVLVSLLVSFTLTPMMASIFLKPHNAPPPKGNVITRWSFWK
                     KAGDFLEKNYKRLETFYGRVLKFSLRHRAAMLFATLMVFMFSIFIMIFGLGKEFLPPE
                     DQGNFIIRLEAPIDYSVEQVEKYFRPAEKMVRDIPEIKSVFYVQGYQGYANRGIMIVG
                     LIPKADREKSQEDIKKIVRMNLKQIPGLKVSAEDVSLVGGGIRQVPIQYSIRGQDLNE
                     LQKYARQITTEFAKRPGIVDVDTSLEAGKPEFKVYIDRDKAADLGVDVATIAEAINLL
                     VGGELDIARYKDEKKGKRYDIRVRLNAEDRESSEAMQKIYVRARDGKLVELSNVVRIA
                     EGTGPSVINRADRQRAITIFASLEGKPLGEARNELDEIAGRILPADYVPKYYGMAEVM
                     QESFIFLLFALILGIIMAYMILASQFESFIHPATVLLSMPLSFIGAFGALFLTGKTLN
                     IFSLIGLILLMGLVKKNAILLVDYTNVLRERGMSRRDAILQAGPVRMRPILMTTFAMI
                     FGMLPIAFAVGEGAETRAPMGIAVIGGLLTSLILTLVVVPAAYDIFDDWQEYFKKRRA
                     KKTID"
     gene            complement(38740..39864)
                     /locus_tag="PLG98_00920"
     CDS             complement(38740..39864)
                     /locus_tag="PLG98_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE31840.1"
                     /translation="MHLKNLVSFIEKKESRRNTVKFIFLISLTAIIAFSLMSCKKEKI
                     VEKEVLTNVRVSTSVKKNIRPYIETTGTLTADEEVTVSSEVDGIVRSVRVEEGSAVGK
                     GTLLAEVNDIDYALDEKRAQAALKQAQANLTNVKAEFQRKEALFKEELITQQQFDDIS
                     TRVALAEADLAKAKATLDTARERLARTKIYSPLRAMVKEKRVSVGDYVRTSMPLFQLI
                     KVDRLKLNFTVGEKELADLKTSQEVDFTVDSFPGRKFKGTVHLLYPHLEERTRTLQAE
                     AVVVNAENLLKPGLFARATIYTGALREVVVAPLTSLLYDDATVSLFVVEGNTARSRTV
                     KTGRKYGEDIEIIEGLNEKEQVVVVGQNNLSDGVKVHVAR"
     gene            complement(39898..41313)
                     /locus_tag="PLG98_00925"
     CDS             complement(39898..41313)
                     /locus_tag="PLG98_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE31841.1"
                     /translation="MMKKSLLCMMTVLATALLSAANAVAQEYTLREIYQKALHTSEKM
                     EIARENVYVAQMSKDKALSLLIPRLTAYGTYNRFTEDKYNTANIMIQPEDSAAWGVRA
                     DQTFSLSARELDALRIAGQSISKSEYDLDWAKSDFLLAVASSFYDVLKAQKALDITVA
                     NLERLTKYYHLVETRMKVGELTKTALLRAQGELSGAKSDYLKASNALKLARAALTRLT
                     GVEPDFRLKEETVSLSEACDIQGLRKSASDLRTDLKSYDMQTKMAGQQVKYARGAFWP
                     SVGLFAIYNRADQDPAGSTFNDENIYGGISLTFPFFEGGLRMAEFREARAKERQARLA
                     YDDLKKSVDIEVEAACLDLDTLKGSLKFLEDQQIFARDNYNAVMKQFENGLATSLDVM
                     DANTLLLTADKNVVDALYNYQLASLTVKKSSGTLLQFVRDGDPAVKGSSGALWQLTDV
                     GDLRAKKSGGRLLLFFSIDES"
     gene            complement(41334..42677)
                     /locus_tag="PLG98_00930"
     CDS             complement(41334..42677)
                     /locus_tag="PLG98_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE31842.1"
                     /translation="MIGKKIRILTIVVMIVFSSRLSFAQEVLSLEASIDLALKNSNIL
                     NIAKEGLNSATAQKREALTGFLPKFSTAYSYSRLNEAPFSRFQGLPPGPMYDLNGMET
                     PVGTKDNYNWIIEARQPLFAGGGILANYQASSIGEEVARLEETARIQDVVRDVEAAYF
                     NILRAQKINEATAQSVEMLKAHRDVAENYFKVGLIPKNDLLFADVELANGRQALVRTQ
                     NAVELAKSRLNTLLKRDISAPVDVMDILAYQPLKQTFEECLETARRLRPEMKISSLKV
                     QQAGKLVRMSQSEYYPALSLVGNYTRFGDEASVHGSDFKDAESWYIMAVANWNFWEWG
                     RTKFRVDASKARENQASESSKELIDQIALEIKNAYLLLQEAQNQISVSETLIKQAEEN
                     FRISEERYKERVATSTEVLDAQTLLTRAKSEYASALGDYNIRLAHLQRAMGNIWP"
     gene            complement(42667..43287)
                     /locus_tag="PLG98_00935"
     CDS             complement(42667..43287)
                     /locus_tag="PLG98_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE31843.1"
                     /translation="MERLERKEREFNMRRAEILEKAEKIFSVKGYHNVTMAEIASASG
                     FSTGSLYQFFEGKENLYSSMISEKLNLMYEKTKKATDRADNVLDKIEMLVDAQLRFLE
                     ENADFCRLFIKGESTALSEAMTSMREKIIKDYFKYIGFIENLLKEGIQQKRLKNLPAG
                     DMAQSLVYLIRAVSMEWMLSLKNESPYSKKGFILDIFFNGVKNHDR"
     gene            43624..43899
                     /locus_tag="PLG98_00940"
     CDS             43624..43899
                     /locus_tag="PLG98_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31844.1"
                     /translation="MRKLMQTTLLTAFVVLVTTSFSYAQYSVTGDANFPFFHLGCLIV
                     GGLIIISLKRKYTKLYMSEAIGSFALYALLVSIFTAPVVDALKNLIN"
     gene            complement(43978..44490)
                     /locus_tag="PLG98_00945"
     CDS             complement(43978..44490)
                     /locus_tag="PLG98_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31845.1"
                     /translation="MDKKENIERINTNIADRFDKVEKKLSGAGSTAELFEMLLTQIEA
                     EFQVPFIWISVADTIKTGRVIDAFKSSRILKDRLNVIKADQFREIFFSGLQPILVNKN
                     LRPYYRLFPPNQKYFIKSLAMVPFKINGEVAGSWNNGDAIQNRYSPDMETSLLKKLSR
                     QLSARLDELL"
     gene            complement(44500..45267)
                     /gene="xth"
                     /locus_tag="PLG98_00950"
     CDS             complement(44500..45267)
                     /gene="xth"
                     /locus_tag="PLG98_00950"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOE31846.1"
                     /translation="MKLATYNVNSIRSRMHIIVPWLKAHSPDFFCMQETKVADTVFPA
                     AEFEALGYHVVFKGDKQYKGVAIVSKQKPQKVIYGFDSEPSDPDRMIIAAFNDLTIIN
                     TYVPQGQEIESSQFTYKLEWLARFKKYLHKNFRPQNQLIWCGDLNIAPERIDVHDPKR
                     LLGHVCFNPDVWKALEDIKSWGLTDVFRKHHPGIAGQYTFFDYRIRDSVKRGLGWRVD
                     HILATKALAERSKSSAIDLDSRLAEKPSDHLILYAQW"
     gene            45775..47115
                     /locus_tag="PLG98_00955"
     CDS             45775..47115
                     /locus_tag="PLG98_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOE31847.1"
                     /translation="MQLIESVLQQISDILWGWPLIVLLVGTHIFLTFRLKFIQRYMFK
                     AIKMSISRKREGEGDISQFGALTTALAATIGTGNIVGVATAIAAGGPGAVLWMWLTGV
                     FGIATKYGEAVLSLKYRITNKEGLMAGGPMYVLERGMNARWLGLIFAALTAVAAFGIG
                     NMVQANSIASLVQETFNISPWITGLTMTVLTAVVILGGIKSIARVCEKLVPIMAVFYV
                     FGCLILLGMHYETIPETFKLIFQSAFSGHAVIGGFLGAGMKEAIRYGVARGLFSNESG
                     MGSAPIVAAAAQTKNAIRQALVSSTATFWDTVVVCAITGLVLVNSGEWIHGYKGAALT
                     KAAFSALPVVGPFVLSIGLLTFVFSTILGWSYYGEKAAEYLLGTKAIRPYRYLWVGAV
                     MLGSVLSLPIVWTFADIANGLMAVPNLISLLVLNKVIISETKHFLWEGKLDEPR"
     gene            complement(47498..48814)
                     /locus_tag="PLG98_00960"
     CDS             complement(47498..48814)
                     /locus_tag="PLG98_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31848.1"
                     /translation="MMKTAILLYHFFKSLIEIHLSARLPYGLQRKLCERRLIRSIRHA
                     YDHVPFYRQKYDAAGVDITSIRSADDIKRLPFVTKEEIRQNFPDGIVARGVNREACRY
                     SATTGSTGRSLPFIFTQATYAFYVALGVRMYTMIGYRPWHKMAYIKYTDLNYTRLGPF
                     FRAVHIKSTIPVEEQIAQLRKAGADLIIGYASLVYEIARNATPDDLKDIRPKFIGINS
                     ELSTEAQRTFISETFGCPVYDEYSTEETWMIASQCRCNNYHIFTDNVWVEFIDAKGQE
                     VAPGDMGEIVLTTTRSPAMPFIRYRIGDMGRFSTAECPCGMGFPLLQSFEGRADDCFI
                     LPSGKFVSSLKLLNTFTMYIKKYLHLLEEFKVTQVHSDLIVIKIVKGNQYDSAHLEEL
                     LADLRRLLDDSVSIKTEFVDVIEKTDGIKRKAIESLVSQKPTGSPS"
     gene            complement(48827..51985)
                     /locus_tag="PLG98_00965"
     CDS             complement(48827..51985)
                     /locus_tag="PLG98_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE31849.1"
                     /translation="MWISDTSIKRPVLATMFILALVVLGLVSYPSIGVDLFPKVEFPL
                     VNITTGLAGASPEIMDVDVTDKIEEAVSSINGVKTIHSLSIDGVSSVIVEFELERDID
                     LAVQDVREQISAVRSQLPDDIIEPVIQKVNIDASPVLWLALTGDKSQRELSTYVDESL
                     KEKLQKIQGVGALSLAGMQKRQIRVWLDSNKLAAYGVSPGDVAAALKRENIELPGGRI
                     ESLTKEFSIKVKGSLKDVSAFNDLIIASYAGVPVRLRDIGRVEDGTQERRSYARFNGI
                     SAVGIGIQKQTGTNTVQVVDRVKEEVERIKKSLPPGMDIRIAFDQSVFIKQSMDQVQE
                     HLILGSILAIIAVFIFLRNFRTTLISAVALPVSIISTFAIMRIFDFTFNNLTMLALTL
                     SVGILIDDAIIVIENIYRHIEDGMSPREAASFATDEIGPAVMATTLAIVVIFLPVAFM
                     KGIIGRFFMQFSLTVVFSILVSLLVSLTLTPMLSSLLLHPRKKESLDSERPGTVMARI
                     GQTLERGYKKIEEAYRPILTFSLDHRRAVLMSALILFIFSLWITRFIGKEFKPGEDQS
                     QFMIRMESPIDYSVEHAGAMFSQAEKIVRNRTEVTSVFYGQGAGQGRLSQINQAFIQT
                     SLKPRSDRDKSQSEIMAELRKEFTAIPGLKTTIEEVAILGGGIRNVPVQYSISGGSID
                     ELRKYAKQIVGELSKIKGLVDIDTSIEAGKPELSVYIDREKATNMGVSVAAVTEAVNL
                     LMSGEVEVTKFKDTAKGRRYDVRMRLDAKDRMSPSDIGRLYVRAVNGELVRLSDIVTV
                     EEGGGPSSISRKDRQRTIWIFANLQDKPLGEAMQEIDAISARILPAGYVAGYSGEAEE
                     MGKSFKYLMFALLLGILLAYMVLASQFESLLHPVTILLSMPLSFIGAFAALLIFGKSL
                     SIVSFIGLVLLMGLVKKNAILLVDFTNTLRERGLSRREAILQAGPVRLRPILMTTFAM
                     VFGMLPVALGIGEGADLRAPMGITLIGGLLTSLFLTLAVVPAAYDIFDDWKENMIRRL
                     GSRGASPWIRPFGKNKKENNNN"
     gene            complement(51995..53191)
                     /locus_tag="PLG98_00970"
     CDS             complement(51995..53191)
                     /locus_tag="PLG98_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31850.1"
                     /translation="MTYSRQLSSTERAYVVYNECCPPFLNQLFWEGTGYFDLPKWREA
                     VKIASEANPGSRVVVKGITAFARLVDTGKTPPVIEVDGTHWDGLGPEGAPAKLSETLN
                     PFEGPTSEVVLIHGNPLRVVFRTMHATMDGRGTVTWVEDIFRGLRGEAPLGSDSRITE
                     FEIAATFQKQGRKGPVPEYIAPTGMPQGDQPGVIWRRRRYPGRYHNVLGQVATLLAGK
                     AWQYQDGKVRLAIPVDMRARVEGLRSTGNLTNLIYVEINPSTTPADVSQDIKRQLEAR
                     HDGMIYWGDRPIRFLPLWLLKKLIKSNIATKNRTGRYPNSGVLTNMGRIPVFSGGGFE
                     GKYTWGIPPGQEYSPIFIGMCNSDDDMTMIYSMPNVLAGGGRLETLMDHIQQGLVASK
                     ADRKKG"
     gene            complement(53188..54393)
                     /locus_tag="PLG98_00975"
     CDS             complement(53188..54393)
                     /locus_tag="PLG98_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31851.1"
                     /translation="MTKRYTRKMTFNDRAFVVLNQISPPTVNQFIFDGEGTLDPEKWR
                     RAADAASEANPGTRLVLRGHLSWSRWVDSGINMRVREVDGRNWDGRGPENEPLLEESL
                     PYRGNPTCEIVLIQGPTPRVAFRSHHAVCDGRGTLFWAEDVFRTLRGEAVVGSTSNIT
                     DVELCKSFQNQYRTPFPAEHIAPTGRAKGNDTGVTWRRCSLKGKMPHLLAQCARLAAE
                     EAWRHGEGVVRFGIPVDLRHRRPELRSTANLSFALYTEVKKETSPDDIARDIALQISR
                     CDECRLTKGDERLEYMPLWMMRYKAKNIIRERHRQGLYSLSGILSNLGRINLNRFCGG
                     GFTARAFWIIPPAAEYYPFFMVMTGYEGGSDLTISMPKVLASEGRLENTLKRIVHGLE
                     GTNQREDTI"
     gene            complement(54390..55199)
                     /locus_tag="PLG98_00980"
     CDS             complement(54390..55199)
                     /locus_tag="PLG98_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31852.1"
                     /translation="MSRALHLAVVMLVTAFSIQTAVAQEHFLHALGHFETLSTTQGIL
                     IAAPHGTYDIRTDVLAKDVARTLGAGYVIAGGFASPSPRINVNRPSEGAGHTCEKERR
                     TERARDVYDQYARLIDKIRDGKPLRLYVEIHGHTSIPFLNRLEIATTGITTEEARRLK
                     DRFPAFLAQAKAAYPAYPDLELRIEPLDKIYFGARCNKTTGYLSTRNMERALHMEIPR
                     PARTPEALKATAALVSSIVRATMTISESASGPGPKRATSDADKKSSGGPAR"
     gene            complement(55325..56353)
                     /locus_tag="PLG98_00985"
     CDS             complement(55325..56353)
                     /locus_tag="PLG98_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOE31853.1"
                     /translation="MDKTAKISNSQAVMGDMDFPHEERARPFLMNTKLWKQLLPLAVS
                     GAILLYLFRHIDIRMCFNAMLTANVSLYIPSLIALILITFLLDTQNLAETLKHFHYPL
                     SWKNAFTLRGVTYLIMTIDYSVGMGVLIYYLKNHLGIPVMRSTGLMTFFNGITQKALV
                     YMAIIGLVILSPASGLLRNLLIFFIGFALLDILFIIVLKKLPSRGLALKIKSMNLLKV
                     FHEAPWRTYGVLIFWRILYYACFVAFFYIAVRAFNMQIPLVALIAYVPIILLVISLPI
                     APGGFGTAQATMLLLFKDYGTPLNIMAFGLIYTTSILVLRYLIGLLYAKHIKGLPAGE
                     DSTSEQRN"
     gene            complement(56445..57818)
                     /locus_tag="PLG98_00990"
     CDS             complement(56445..57818)
                     /locus_tag="PLG98_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31854.1"
                     /translation="MIKLQKGYLKISCVLMCLLALIFATRTWACDVAVVSGKVTTDGK
                     PLIWKNFDNSSSDRQQVAYFPAKKSGPGGYLMVYRFENGMKLLNNGSAITPSGGVNES
                     GFAIAGTSVYQDYNLMAEPVNINTVLMEEALATCTTLAEFESLLKRWPYYHWGTVISA
                     NFVVIDAKGGAALYECFTGHLNTGLNLMQFRKNDANTGKITNQYGWTLKRAQSNFIGF
                     HIMTNYNYYIPWNVGQDRKYRAEALLTQLATEKRINYRTVMREVAKDVVGKQVQTDLS
                     SETNYSTTYCLSRNATRSSIVVRGVASGDNPRLATMWVNLGEPSVGVFVPFFAAARGT
                     SELARVDTYANGAWRDYDDSCLLNQAISARETYGSLIYSSNEGDVIWGMYDNYINKVE
                     LAKVQQWTFQIEDTIIDNAESFLSSLQSNPAQITAFNLKDFADYCADYAYENFETGSA
                     DAFPWQY"
     gene            complement(57904..58857)
                     /locus_tag="PLG98_00995"
     CDS             complement(57904..58857)
                     /locus_tag="PLG98_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31855.1"
                     /translation="MKSMSVSSASEQRGHQSDSTDPLPVNAQDFFIQSHKGFNYYLID
                     LNTAEWNRLGDIIRGCEAVENDCWKAQEEFSTYLRKCDVLSYVEKNGRIVAFDAVSIL
                     NSGPHCVYNNEETMVLKAFRGRDLAQRLVIMTVEWHLTKAASIKNKDYFIFTSISANP
                     AVVNRYFNKSWLRVLFDSSFSPSPALIALKEEYCRKYGIGLVDQSYPFCLKNMFPGSN
                     GFDPNDPKYQFSPHVRANMPADFEHMDRGDAFAFMLMIPMKIVRLVTFVLMIKCFGKE
                     YFSSRGVGLFSSRRPLIPSFTDRRVPGLSGEPFSVKTADHG"
     gene            59834..61576
                     /locus_tag="PLG98_01000"
     CDS             59834..61576
                     /locus_tag="PLG98_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE31856.1"
                     /translation="MNKRKKLGEILIEDSIVSEGQLMSALALQKKSGKMIGQTLIELG
                     YITEAQLVEAFRKKFSISYIDCDGVVVPEKFLKLVSMTMAVKKKILPTDVSGNTLFLA
                     MVNPLDYSTIDEVKFLTGKNITPLLTTESSLMKAIEKNYGYCSIEKIWKPLNDIHLDG
                     EMQFNLDKSIEFHKMEEEDESALRADDLYKIGEEPPIVKLVTLILVNAVKSRATDIHI
                     EPHEKYVSVRYRIDGDLSTVLKISKTYQEAMTSRIKIISTMNITIRRMPQDGRSTVKY
                     EGRMIDLRVSTIPSAHGETIVIRILDSVKGLIPLSKLGMPDNIIRDLLEMLNKPQGML
                     FVTGPTGSGKTTTLYSLLTQLSLESESVVTIEDPIEYELENIKQVPVNEKVGFTFAAA
                     LRSILRQDPDVIMVGEVRDSETAQISTKAALTGHLVLSSVHTNDTIATISRLRDLGIE
                     PYLISSALNAILAQRLVKKICENCRVEFEIPQYLFENYPYLKKLKIGISYKGKGCEAC
                     GFTGYKGRIGIFEFLRVDGNLRRTIPLYHSDQDLCIVARENGMKTLIEDACEKVEHGL
                     TTINEVLTKIHYYE"
     gene            complement(61607..62047)
                     /locus_tag="PLG98_01005"
     CDS             complement(61607..62047)
                     /locus_tag="PLG98_01005"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOE31857.1"
                     /translation="MRSDIQKIIQENRMCVMATVGRDAPHCSLMSYASGRDGREIYMA
                     TFKDTKKYHNLIANPNVSLLIDTRVVDAEGTPRALTVTGIVEAVDNDKSIEEIRETLL
                     RKHPDLRDFFNHPDTRMIVVRPTTLQLLDNVTDAYFEELRQPKR"
     gene            62179..63303
                     /locus_tag="PLG98_01010"
     CDS             62179..63303
                     /locus_tag="PLG98_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOE31858.1"
                     /translation="MKNKKPFLTEVRSQDVLSVSIPDTRSFAGAQRKSFRVWWIAFRY
                     HFVPASFLPAILGSVGAWVQTGLMNRWFFFLTVLGVTLNHIALNMTDDYFDYHAAVDQ
                     GGERRENPYTGGSGTLTGGLIKHGHMRLMFTLFYLITIGIGLYLCAAQTWWIIVFGLF
                     GVGCSYFYTAPPIRYGYRGLGEFSQLINFSFTIGMGAYVVQTVSFSWEIFWILLPLGF
                     MMFAMIVINEIPDRFDDAAAGKNTLVVRFGLKAAARFYGVAMMLAYAVILLAPPLNMA
                     GYWIYLGLLTLPWSVRAFLIMKAYGNEPDKLAPANLLTIRVHNFTGILLIIACLIQGL
                     QDRQVFQPVVFAAVILSAFYLPAAISIFFKKMPDGRRTEA"
     gene            63384..64103
                     /locus_tag="PLG98_01015"
     CDS             63384..64103
                     /locus_tag="PLG98_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HOE31859.1"
                     /translation="MKMDKETLSKHDGREGHKAYVAVEGRVYDVTGNRLWKNGVHMNR
                     HQAGTDLTEAIAASPHGKDVLSKLQDMGQMSPEKVAKPPFIPLWMHHWLEAYPFFKRH
                     PHPMVVHFPMAIFIIAPLFLAWYYLINPVPGLPAAVFYLYILGTLSLPAAIATGLLAW
                     LVNYSGQPNPLIIRKTIFSLLLLLCDGIVMAALIISPAILQDPSGSEWMIPVLIFLGL
                     PLASIIGEHGGRLVFPVLKNK"
     gene            64390..64848
                     /locus_tag="PLG98_01020"
     CDS             64390..64848
                     /locus_tag="PLG98_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOE31860.1"
                     /translation="MPSKKLKEFLNSQSVKYVIIAHSTAYTAQEIAQSAHIPGTELAK
                     TVIVYIDGKMAMAVLPASYSIDFDDLRKETGAKKVELASEAQFKDMFPDCEIGFVPPF
                     GNLYGMDVYVDEHLTRNKEIAFNAGGHLELYRMSYRDFEELVKPKVLKFT"
     gene            64914..65645
                     /locus_tag="PLG98_01025"
     CDS             64914..65645
                     /locus_tag="PLG98_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOE31861.1"
                     /translation="MELNIIRKAMAHKTISLHVLLTVFLVTNYSAITGAAETGKKSLL
                     PDRSVITLPAPVYQGRMSVEEALRKRESVRQFSQSPLTEQELAQLLWAAQGKTRDWAA
                     RTAPSAGALFPLEVYVVIREGVFRYSSGDHRILRVVKTDVRRQLAAAALGQDIIRQAP
                     AVFVIAGVYKRTAEKYSNRAKRYVEIEAGHAGQNLLLQAVSLGLGAVPVGAFQDAHVK
                     QALHLPVNHEPLYLIPVGRKRFVPP"
     gene            65807..66097
                     /locus_tag="PLG98_01030"
     CDS             65807..66097
                     /locus_tag="PLG98_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31862.1"
                     /translation="MGDFDKRKYIRYPAEPNELVTVYYMDEKTTKTGQRVGLGENESF
                     KGCCVVFVGDVDFKQGQEVFCECGTLPKTKAQIVWVKKLENQITKVGISFRE"
     gene            complement(66387..66992)
                     /locus_tag="PLG98_01035"
     CDS             complement(66387..66992)
                     /locus_tag="PLG98_01035"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE31863.1"
                     /translation="MSGKTIHEKAEYRSAIRSRKLIKQAYVSLIKEKDIDKITVMDIV
                     TTADINRGTFYAHYSNVNAVGEQIGNEIISALLEFLDEFKNSRLIENPLPFLMNIARF
                     LEKDLEFYRILINSQRSIIFLNKLKGIFLDKIVSDEKKMSAIKNKEQFMVCVNLYASG
                     FVGLYQDWFNNRIKMSLDDLTLHFSEIIKNGFHAFLPKKNR"
     assembly_gap    67853..67952
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(67991..68938)
                     /locus_tag="PLG98_01040"
     CDS             complement(67991..68938)
                     /locus_tag="PLG98_01040"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOE31864.1"
                     /translation="MDKKISGQDAEKRMTPLQRDVAFGNATEPPFQNEYWNNHQEGIY
                     VDIVSGVPLFSSKDKFDSKTGWPSFTKPIKDNAVWRKTDFSHSMVRTEVRSTQADIHL
                     GHVFDDGPAPTGRRYCINSASLKFIPREKMETEGYGEYLSLFAAPSGKSTETAIFAAG
                     CFWGVEEYFSRVKGVVNSESGYTGGTVKAPSYEQVCSGKTGHAEAVRVTYDPTIVTYE
                     RLLQHFWELHDPTSLNRQGNDVGTQYRSAIFYTNPQQEKAARASAEKLARSGRYTRKI
                     VTEIQPAKDFYPAEDYHQDYLKKNPRGYCHIDLKKAKKD"
     gene            complement(69134..>70442)
                     /locus_tag="PLG98_01045"
     CDS             complement(69134..>70442)
                     /locus_tag="PLG98_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE31865.1"
                     /translation="SSESGVDLNRSRLSQIKGISFLDDGGGYVFTGTRPPISDLDGLA
                     DCDLTLIKYLQKRWSYIPINRGRGCNWNCEFCVVNKQYGKYKAASIDTALRQLIRYAD
                     MGYTKFFFTDDNFAQHPDEAIALCKAIGHYKRNFLKKIDLVVQVRSEVAENDALIEAM
                     HFAGVSMLAIGYESPINEELRAMRKGVTVEKLIARSRKLSNVFYLHGMFIFGYPLNDD
                     AQGPSSFTVKQRAKIYKDFFKKSGIDTIQVLNAVPLPGSDLRTRLEREGRLLPLKIVG
                     WDKYDGLFLCYDPRPDSMDAFELQGMPGLLMKKKYLGGFIKRNLNYGNWLNWTYIATI
                     GFPIQFVEFYIRRFIRNLAERQRAGKRPDGAWSPRTIFAVPLIIAWKDIKRIWRNLFV
                     RTYAGAIVNRWLKIYRKSRYGDKLRKFFLDSKMTPKSGHRLNT"
BASE COUNT        18188 a        16522 c        17771 g        17861 t
ORIGIN      
        1 gttttattat ctatcttata taaaaccatt tacacagtta gatttacact ccctttttcc
       61 ccccgatgtt aagctttatt ccaaaggaat ccacacgggt tattttacca tttcaaattc
      121 cgccagtttg atttttctta tcatttcatg tctgaagact tccggttttt cggaagtatc
      181 aatcttaaag cggcaatccg ccgttatctc atcgataggt tcaaaattat ccttctgctg
      241 ttgaaaaagt tcccatcgtc cgtctgatac gttatcgtta tccagaactc tcttttcaag
      301 tcttttttgg gtgatgtcgt ccggacagaa gcattccagg acaaaaaacc ggatctgaag
      361 ttttttagcc agcgccaggg cctttaatcg ctccgtccgt tttttaaagg aagcgtcgat
      421 gatgacagcc tttcccagat ttattttctg ctcggccagc gccagggctt tttcatacac
      481 cagacgggaa atatcatcgg aataaatgcc gtcaccgaag cgctcatgcc gtctttcggt
      541 cgtcctgatg ttgaacaatg ctttgcgaag ctcgtcggta cggataattt ccgttcccag
      601 gcgcacggca agacggcggg cttgataact tttacccgat cccatcagcc ccgcggtcag
      661 tatgagcacc ggtttatcca gtcgggcggc gtatgtatag gccagatcga aatagtgttc
      721 tgccgtttcg gtgatgtccc gttgttcgtc tgcagttgta tcctcttgca gcagacgaag
      781 actggtgacc ttgccgcgga cgtaagcgta ataacaccga tagaaattca acagtttcag
      841 caggccggta tcaccggaat atttcaagta ggattgtata aaattttcgg catgatgcgg
      901 ccagccgttg aaatcgatgt ccatggtcaa aaaagccaca tcttcggcga catctcccca
      961 gcgaaatcgt tcattgaatt caatacagtc gaaaatggtg atctcatcat tgatacagat
     1021 atgatccagg tgtaaatcgc catgacagtc tcggatcttg tgatcggtga ttctctgctc
     1081 gaaatgtttt ttattctcga tcagaaattt atttgcaaag tctttaatca agctgtattg
     1141 atattcggaa atggtgacat cgataaattt ttccgtttct gcaaaattct cttcatgatt
     1201 gtggcgaatg ttttcgatgt cgcccatttt atcgatgcgg ctgtccgttc gggcctgctg
     1261 atgaaacaag acgattttac gggccagggc atccattatt ttttcatccg ccgttcccct
     1321 ggccagaagc gtcttgagca tcctgtcgcc ggggagtttt ttcatgcgca ccgcataatc
     1381 aacgatctcg atgccctgat caagggttat atcgcccgcc tcattacgtg atagcgagac
     1441 gacatccaaa tatgtgtcgg gggcaagtct tctgtttaat ttcaattctt cttcgcagaa
     1501 aaattttcgt ttctcccgcg ttgtgaaatc cagaaaatca aactttaaag gttttttgac
     1561 tttataaaca taatcgccgg cgataaaaac ataggaaata tgcgtctgga tgaattccac
     1621 attagccgga cgatggggat aaaaatccgg tttctgcatg gcttcgacta atttgagatg
     1681 cgtcatcgtt tatgcccgtt gtaaaataat ttctaaaatt tttttaaaca atcgcgaaac
     1741 cataatcaag gcaagaatca ggaagttgtc gagagtccgg tttcgcatag cggtacacgg
     1801 gattatatac aaaatcgaca ttccgggact gcatcgttga atgtacgctt caaaatttta
     1861 tgcttcttat tataccacga tatgccctat ttgatagcat tcatctgcgc gacacctccg
     1921 gagacggagg tgcgttgaca gcatgctgaa ttaaattccg acgtatacgg gaaaaccttt
     1981 catgattatt ttagaaccgc aaaaaacggg aaaaaggaga tgttgaagag gctgcgtgat
     2041 gtggcagaaa ccgtaaccgc cggaaaaatg atattccggt tggtccgatt ttattgctca
     2101 accttccgtc tgacgataga aaatgaattc gaatggatcg atcattatct gcacagagga
     2161 aacattctga tttgtccgtt gcatcagcaa ttcctttcga cggtttgttt cttttaacga
     2221 tattgatatt attatcctgt cgtcatgatt tcccaaagtc aggatagatt catgattcca
     2281 aagccgtttg cccgtgtccg gattttgttc agggatgaaa ctgcagaaag acgtggatgc
     2341 agacgactat catcacaaga tgaaactgct ggggaccgtt atgaagccgc tgcgtcagtc
     2401 atgaaagaat gttccataca tcgtcttcag caatttttgc ttttcaggat gctattcgta
     2461 ttgttcgagg aatttgtgca acgcattttt cagccaggca aatgattcct gtttggtgag
     2521 cacaaacatg aagccgctga tattcttttc gttaaaagaa aaaaccgttc ttaaaacgat
     2581 cgccatacta tcggggttga taatgctttc gtaaacgcga tgtaggcttt tttcaacgag
     2641 aacgcgggga ggcgaatacg tggcaatgtc gcccaaaagc tccgcgagtt ttccgatgca
     2701 tgcgccgatc aatatattgc ctatttccag aaaggtttct ttttcctgtt ccagcgaggg
     2761 atccgattga aaaaattcat ccttgccatc aaaaagagaa atcatccttt ttccggttcc
     2821 ggaaggaaaa actaaaagag cgttgccctt gaatttgccc cagaaactct gttcgacgac
     2881 ggatacccga tcctggtcat agtccatgat ttcactattg atatagtccg gaagctggta
     2941 tgcaggcagc aatttgatct gcggaacgct gagaacaaca aaaatatcta taaattcagc
     3001 caggtccgag gcggcccggc cgaaagcgat attcataatc tcctgaagaa gatcggtttc
     3061 tgcgcttgta atcaaaccct gaatgttatc catttgcgac ataatttacc cacgggaagc
     3121 cagggcttct tctgcttctt tgatagcccc tgcgacagat tcttttgaag gcggtttttt
     3181 taatactgaa aaagcgccca agtcatgtgc tttggcaatg gccttgattt gcacgtcggc
     3241 ggtcagaatg atcaccatgg cttttgtatc cattttcata atttcttcca aagcctgaaa
     3301 tccggtcatg accggcatgg ttaaatccat aaaggtgagg tcgggtttaa tctccttgta
     3361 tttttccaca cccatttgac catcggccgc atcgtacaat tcatacccca tatctttcgg
     3421 aacgcaactt ttaatgattt ttacggatat cggtgaatcg tcgacaatca aaattttttt
     3481 aatcataaaa acctcttctg cgtgtagtcg ttaccatata atacttcatc ggaaaagaag
     3541 aattttatta atttgttttt taacatacgg caatataaaa taacattata ttttataaac
     3601 agattctata gattttttca atagtgtttg tcttccggca tgaagatttt tttggatgat
     3661 gtatgattaa tattttcgga acagctatcg gcgattcgca acggttgatt gcttgacggt
     3721 ttcggtgtgc taatgggggg aaatactata attataaacg aattaggaac ataacaatca
     3781 tattgacaaa aaacgtaaaa aacgtaatga ttaatgaaag ccattgttta ttcatgggaa
     3841 tactctcttt tgtgtcaaac agattcttaa aatttaattt ttgatggggc aattatctcg
     3901 aaaatgaact taaatcgtct ggccatatca ttgatagaac tgaatcctga aaatcctgcc
     3961 gacttagaag aatttaaaaa aaatcttctt tcctgttaca acaataccca aggacgtatt
     4021 cagaatattt tgaaagaagc ttgttttgaa gcggatgcta tggtcgacca ggttccggaa
     4081 cttcagcgca acattatagc ccgtctggga gttttgctgg aacaggcaat gggaagcgat
     4141 gaaaccgata tttctgcaac gacggaacaa acgtctcagg tagaacctgc ccgggaaccg
     4201 gacagcattg acgtccagca cgttatgttg tccgccgatc ttgacgcgga tttattgaat
     4261 gaattcatta tcgaaaatat ggaatgggca acgatggccg aatccgcaat tctggactgg
     4321 gaacagaatc ccggtaacca ggaattactc aatacaattt ttcgaggatt ccacaccatc
     4381 aagggaacat ccgcctttgt caatctggat tgtgtcaaag atcttgccca tcaggtggaa
     4441 tccgtgctgg ccaaagtcag agatggagaa catgttttca cgagaagcta tgccaatctg
     4501 gctctcaaat cactggatgt gattaagagc attctggaac aaatgaaatc atccggtccg
     4561 ggccagcaga tcgaactccc ccgcggttat cagggattac tggaatcact gagaaacttt
     4621 gttcccgatg agcaggtgca ggaaatggcg attccggcag aggaatcaat ggtcgaaccc
     4681 gaagcggcgg ctccggctta tacgaaaacg aacagaggga aaatagcaaa aaaggaaggg
     4741 gaggcatcgt cggaacaatc cgcttcctca aataagattc gcgagcagat gaccgagtca
     4801 acggtcagag taaaagtgga ccggttggat aaattgctgg ataccgtggg tgaactggtc
     4861 attgcccaga ccatggtagg ccaggacaat ctgattgtca gcggcaaata tcatgagttg
     4921 aataaaaaaa tatctcaggc cggaaaaatc gtccgtgaac ttcacgactt gagcatgttt
     4981 ttgcggatgg tgcccttaaa gggcacgttc cagaaaatgg tgcggcttac ccgggacctt
     5041 tccctgaagc acggtaaaat ggtaagctgt attaccgaag gagatgatat cgaaatagac
     5101 cgcaacatgg ttgacatggt ggccgatcct ctgatccatt tgatccgcaa tgcggttgac
     5161 catggcattg aatttccgga ggaaagacgc cgggcaggaa aatcagaaaa tgggcgcatt
     5221 tatctgacag ccggccatgc tgagggcagc attatgctga cggtctggga tgacggccgg
     5281 ggtatgaacc gtcagaccat cgtgaataaa gcgatacaaa aaggattgat agattctgcc
     5341 gaacgcatga cggatcagga agtctggcaa atgatatttc tgccgggatt ttcaacggct
     5401 gaaaaggtga cggaggtttc cggccggggt gtcggtatgg atgtggtgaa gcaggccgtt
     5461 gaagccctga ggggccggat agaggttcaa accactgaag gtcagggctg taccttcatc
     5521 atgaggatgc ctctgacgat ggccattacc gacgggatga tcgtcaaggt gggctcccaa
     5581 cgatatattt tgccgacggc aaatattcac aaagcggtca ggccgcagga acccgatatt
     5641 cacacagtta accagcgagc ggaaatgctg tcattccggg atgaaatggt tcctgtcatc
     5701 agactgtatc gctttttcga tatccctgat tcccttaccg atttaacatc cggcctgctg
     5761 gtggtgatcg gcgaagggaa acaacgctgc gctcttttcg tggatgaact cttagggcag
     5821 acccaagttg tgacgaaatt gctgggcaaa ggaatgaata acgtgccggg catagccggt
     5881 ggagcgatta tgggcgacgg aaccgtgggg ttgattctgg atgcctcggg aatcgtcagt
     5941 atcgcccggc agcagaccaa tacaacaact taacaaagag gccaaacagg aggagaacgc
     6001 atgaacaatg caaatgcagt aaaaaacgat caatctgcca atgccgattc ccgggccggt
     6061 aagtatctga ctttttttct ggccagtgaa gaatacggag tcgaaatatt aaaggttcag
     6121 gagattatcg gccggatgcc gattactcct gtaccgctga cgtccagata tatccggggc
     6181 gtcatcaatt tgcggggtaa aatccatccc attatggatt taaagatcaa attcggcatg
     6241 gaggaaactc agattaccga cgagacctgt atcattgtca tcaagaccac cagtatgatg
     6301 atgggtattc tggtcgataa agtttctgag gtgattaata ttgcatccgg ggatattgag
     6361 gatacacctt ctttcggcgg cgatgttaat ccggaatatc ttttgggagt gggcaaaacc
     6421 ggcggacgcg tccgcctgct cctggatatt gaaaaagtca taaccgcctc cgatattatc
     6481 aatatgaaga aggctgctgc cggagaaggc aaagacgcca tggccgagtt ataggacgtc
     6541 aatattccat aacgatttga aactgacgct acaggaaggg agataagcat gctgaacgat
     6601 attaaagtgg gagttaagct gataggaagc attgttaccg tttgtcttat agcggcggca
     6661 gtgattctat acggtattaa agtacgaaac gacgtcgtgc acaaagtgga ttcggtgcat
     6721 cgccagtatg aaatcaatat ccgtgacatc gggaatgtca gagtcagcct cgaaaaaatg
     6781 ggaggagaca tttatcgata ctttggtgca ccggcggatc ggcagaagat gagtaaggaa
     6841 atcaatgaga ccatcagttc ggtggatggc acgatgcagt cctataaaaa tagaagcatc
     6901 gatgttgatg aaaagaaact gattgccgat tttgaatcgg cgtggtccga aatgcaacgc
     6961 cgttacaagg aaggcatgaa ggcggtggat gaaggcagac agaatgacat aaaccagttc
     7021 ataggggccg gaagcgctgc tatgcaggca agggagaaaa cctcagctac gcttcgcgac
     7081 ctcaataatt acagtgtcaa taagaatgaa tcggcggtta aagcaacgat tgacaatgtc
     7141 ggggggcttt cgagcatcat gctgatctta tcggcgattg cgatagccct ggcgattgtc
     7201 acgatcgtct ttctgaccga atccattacc aaaccgctga aaaaaggcgt gcagatgatg
     7261 gcggagatga aaaagggaca cctttccaat cgactcgaca ttaaacgcag ggacgagatc
     7321 ggcctgctgg ccaatgccat ggatgaattt gccgatcatc ttcagaaaaa tatcatattc
     7381 aacatgcagc agatatccga gggtaatatt aatgttgccc cggtcgttat tgacgaaaag
     7441 gatgagatcg gaccggcact gaaaaaaatt atcgaaacca tcaatagtct gctcaatgag
     7501 gtggacatgc tgaccaaatc ggccacggaa ggcaaactcg atgtgcgcgg taatgaacag
     7561 gactataaag gagcttatca gggtattgtg caaggcgtca accggacgct ggatgcggtg
     7621 attgatccga tcaatgaagc gtctgccgtg ctggagagaa ttgccgacaa agacatggca
     7681 gcgcgcatgg tcggcgacta taaaggcggt catgccaaga tcaagaaatc gcttaatctg
     7741 gctgttgaaa atctggacag agctttgcaa caggtggcca tcggagcgga tcaggtagcg
     7801 tcggcaagcg tccaggtaag caccggcggg cagtcgttat ctcagggagc cagcgagcag
     7861 gcgagttctc tggaagagat ttccagcagc ctgcaggaaa tgtcgtccat gacgaagcag
     7921 aataccctga acgcgagaga ggcaaaaggt gtggccgaag gtgcccggga aagcgccgat
     7981 aaaggtgtgg agagcatgaa ccggatgtcg tcggccatca ataagataaa aacgtcttcg
     8041 gatgccacgg cgaagattgt aaaaaccata gatgagattg catttcagac gaaccttctg
     8101 gcgttgaatg ctgcggtgga agcggcgcgt gccggagatg cgggaagagg attcgcggtg
     8161 gttgcggaag aagtgagaaa tctggcgatg cggagcgcgg aagcggcaaa gaacacggcc
     8221 agtctgatag aagaggcggt cagaaattcg gagaacggtg tcactatcaa cacggaggtt
     8281 ttaacgaatt tccaggagat caccgagaag atcaacaagg tcagtcaggt ggtggcggag
     8341 atcgcggccg catcggagca gcaggatcag gggatcaacc aggtgaacaa ggcggtggaa
     8401 cagatgaacc agctgacgca gcagaacgcg gcgaatgcgg aagaatcggc aagcgccgcc
     8461 gaggagatgt cttcgcagtc cgaggaaatg cgggccatgg tcgccggatt taaactcacg
     8521 ggttcaggat atactgaaag agaaagcatt gcctatgaac caaaagactt ctgacagaat
     8581 gttcatttaa ggtgaacaag aagaaattta ttctctgcag acaggggatg cctttattga
     8641 attaaaccaa gatagtataa gtgaaacttt aatcggaggg cgaaatgatt aataacatga
     8701 aaatgggggt taagcttgcc ggtgtatttg ctattatttt tgtagtggta acggcgatga
     8761 tgctatctgg tgcggcggta agaatgaaag cattgcgtat gctcgattca aactatcgtg
     8821 attatgtgaa cgatataaaa aatattggaa ccatcgaggt cggtctcgtc aatatggaga
     8881 aggatcttca tctttatgtc aactcccctg cggcgcggga aaatgctctc atacaaattc
     8941 aggaaggaat cacatctaca gagaattctt ttcagtcctt gaaaagctca aaaagagatt
     9001 ctgaagaaca gaaatatctt tccgaatttg aggtagcgtg gcctgaaatc cagcgcggtt
     9061 ataaggcggt aatcaaagcg gtggatgaag ggaaaaatga tgatgcaaac agtattcttg
     9121 ccgataaaag ttatttgaac gaagcaggca gaaaagctct tgcggtagtt aaaaacttga
     9181 atcaatacag tgtcaacaaa aacgaaacca gggtgaaaca gtacgtcaaa ggcaggggcg
     9241 gctggtccgg tattttgtgg atattttcag gcttcggcgc tcttgcctta ataggtttgg
     9301 ggacgatggt tcatagaaac attacaaggc cgattaagac aatcgtattg atgatgcagg
     9361 agattgaaaa aggccatctc tctaaacgcc tgaaaatgaa tagaaaggac gaaatcggca
     9421 gtttaagccg atccatggat atgtttgcag acaatctgca aaaaaatatt gtatacagta
     9481 tgcagcagat atcagagggc aatatcgatg ttgagccgaa agtgacggac agtaaggatg
     9541 aaatcggtcc cgcacttatt aaaatgatca atgccatagg ttcgatgagc aatgaagtca
     9601 agaggtgttg cctcaacgct ttggaaggta atcttcaaaa cagggcggat gtcacgcctt
     9661 atcagggatc ttttcgtcaa atcattcagg cttttaacga tacgctggat gctgtcatga
     9721 atccgatcaa tgaggcggcc ctggtgctga cgaaggtagc cgacaaagac atgaccgcgc
     9781 gaatggccgg cgactataaa ggcggtcatg ccaagatcaa ggaatccgtt aatatggtcg
     9841 tggacaatct cgacggggct ctgcaacagg ttgcgatcag tgcggatcag gtagcgtcgg
     9901 caagcgtcca ggtaagcacc ggcgggcagt cgttatctca gggagccagc gagcaggcga
     9961 gttctctgga agagatttcc agcagcctgc aggaaatgtc gtccatgacg aagcagaata
    10021 ccctgaacgc gagagaggca aaaggtgtgg ccgaaggtgc ccgggaaagc gccgataaag
    10081 gtgtggagag catgaaccgg atgtcgtcgg ccatcaataa gataaaaacg tcttcggatg
    10141 ccacggcgaa gattgtaaaa accatagatg agattgcatt tcagacgaac cttctggcgt
    10201 tgaatgctgc ggtggaagcg gcgcgtgccg gagatgcggg aagaggattc gcggtggttg
    10261 cggaagaagt gagaaatctg gcgatgcgga gcgcggaagc ggcaaagaac acggccagtc
    10321 tgatagaaga ggcggtcaga aattcggaga acggtgtcac tatcaacacg gaggttttaa
    10381 cgaatttcca ggagatcacc gagaagatca acaaggtcag tcaggtggtg gcggagatcg
    10441 cggccgcatc ggagcagcag gatcagggga tcaaccaggt gaacaaggcg gtggaacaga
    10501 tgaaccagct gacgcagcag aacgcggcga atgcggaaga atcggcaagc gccgccgagg
    10561 agatgtcttc gcagtccgag gaaatgcggg ccatggtcgc cggattcaga ctgacgaaga
    10621 ccgttgcatt aacggccggt ccggatgcca ttaaaaaaac ggaatatcgt ctgccggatg
    10681 tcgctccagg gaaaaaaggt gataaagcgt cagccggtct tcatcctgat ccccgcaagg
    10741 tcataccgct ggatgataag gatcatgata tattgcagaa tttctagttc gtaccggtcg
    10801 ttatgaaatt tttttcggac aagtagaaat ctgccaacac aaaagaaaag atattatgca
    10861 ttcggaatgg aataatcttt cgacggatta ttattctcta aaacttactt ccgagcaatt
    10921 tgataaaatc agcagactgg tttatcagat ttccggaatc gatcttcacg aaggcaagga
    10981 agaactggtc aaggcccggc ttatcaaacg gctcaggcac ttgaaatttc atgattttaa
    11041 tcagtacctg aagcatattg ccaacgataa gagcggaacg gaaatcgggg cgatggtgga
    11101 catcctcacc accaacaaga cgaatttttt cagggaatca gagcaccttg attttttgaa
    11161 acacgaaatt attcccggtt gggttaaggg tccggtcaga atctggagtg ccggatgttc
    11221 ttcgggtgaa gaaccgtaca cgattgccat cgttttattg gaatccattc ctgataccgc
    11281 cacactcgat attaaaatac tggcgacgga tatctcggat aggatgatgg aaaaagcccg
    11341 gcagggtctt tatgatgagg aaagtctcgc aataatgtcg cgtcacttga aacacaagta
    11401 ttttcaaaaa acagatacgg gatcaggtta taaatacaaa gtgattccca aaatacagtc
    11461 gatggtcagt tttgccaagc tcaatctgat ggaacactgg ccgatgaagg ggccttttga
    11521 cgttattttc tgccgcaacg tgatgattta cttcgacaaa tcgacacagg aaaaactcgt
    11581 gggcaggttc tggcatcttt taaaagaaga tgggtatctc atggtcggac attcggaaag
    11641 cctgagcttc ctccaacacg agtatcgtta catgaggccg gccgtttatc aaaagatcag
    11701 gaaggcgcat tcggtcgtca aaaaaaccgg aaaggttaga acggacgtga agtaatatgg
    11761 tctatacagt tggagttgcc gacctgaaga tatccgacga gagtgccgat atgttgatta
    11821 catatgcgct gggcagctgt ctgggggtga cggtttacga tttcagaaaa aagacgggcg
    11881 gtttgttgca ttgtatgatg ccggattccg ggatcgacca aaacaaggca gcaggcaatc
    11941 cttatttata cgtggacagc ggaatgaaag ccatgatgga taaattttat cagaaaggtt
    12001 cacgaaaaag cgatctgatc atccgggtag ccgggggatc aagttcaaaa ctcaacgaaa
    12061 atgaggattt tttccagatt ggccgccgca attttgtcag tttacggaaa tatttatgga
    12121 atgaaggtct catgctgaaa gcttatgacg tgggcggtta cggttcgcgt accgtcacgc
    12181 tggaattgga aagcggaaag atgctgatta agtctcaaac tttcctcaaa caactttaag
    12241 gatttcttgg aatgagaaat gaaatgctaa agagattaga caggagagag tgaaatggcc
    12301 ttaaatactc tggtggttga tgacagctct gttatgcgaa ctatcatcat aaaaacattg
    12361 aaactggcgg ggctgccttt gggtgatatc tgggaagcga aaaacggaga agaaggactg
    12421 cacgttttac aggatcactg ggtggatttg gcgatgatcg atattcacat gccggtaatg
    12481 gacggtgagg aaatgctgat aaaagtcaga gaaaatgatc agtataagaa ccttccggtc
    12541 atcgtcatca gttcggaaag cgatccgggt aaaatagaaa aaatgctgga attgggcgct
    12601 acatttatcc ataaaccgtt tacaccggag gttcttaaag aagttatcgt tgaagttacg
    12661 ggggttagcc atgaaaacga agattgaaaa gattctggaa gaagccacga ttaaaacgtt
    12721 tgaagatatc tgttttatgt accttgaacc ggaactcaaa gacagccagg ctgctctgga
    12781 gccggatgct gctgctgagg ttgaatttca tggcgcttac aacggacgtt tggtgatcgc
    12841 aagcagaggg ggcttgtttt cagccattgc ttcgaatatt ttaagtagcg atcatccaag
    12901 cctccaggag aaaaaagatg ccctcggcga gattgccaat atcatctgcg gaaacattgt
    12961 gccgtcgctc ggtcgaagaa aagcgggagg atacaagatt caaagcccca gacatctcgg
    13021 cagggatgaa ttgctgaaag aggaaaagag taaaccttcg gccgtcgtga ttttaaattt
    13081 taatcagggg cgggcagata tcaaattatt tgtggatgac tacatttcgg accaggagat
    13141 aaacgattga tcaaagttct ggttgttgat gattcggcgc ttgttcgcaa agtattgacg
    13201 gaagagttgt cccgccataa agacatcgaa gtggtgggag gcgccattga tccttatgtt
    13261 gcgcgtgaca agattgtccg tcttaagccc gacgtgatta ccctggatat tgaaatgccg
    13321 agaatggacg gcctttcctt tctgtccaag ctgatgaaat tctatccgat gccggttgtg
    13381 gtggtcagtt ctctgacccc ccgcgacagt cagaacgcga tacgagccct ggaactcgga
    13441 gctgtggaag tcatctgtaa accgggatcg agtttgtcca ctgcagatct ttccgtacgc
    13501 ctggtggaag ctatacgcgc ggcagcacgg gccaaaattg ccaaaccggc tgttgaattg
    13561 gtggaaagag aaagcgttcc gggatataaa attcaactca agacgaccca taaggtcatt
    13621 gccatagggg catccacagg cggtaccatt gccattgaaa aaatcctcag ccggctgccc
    13681 atgaatattc ccggaatcgt cgtggtccag cacatgccgg cgtatttcac ggccagtttt
    13741 gccgagcgac tgaatgcgtt gtgtaaaatc gaagtgcgcg aagctaaaga cggcgatacg
    13801 gcggctcccg gactggctct gattgctccc ggcggatatc atatgcttct ggagcgcagc
    13861 ggagcatttt atcaggtcag aatcaaggga ggcccgatgg ttcatcatca gaaaccaagc
    13921 gtcgatgttc tttttcattc ggtggccaaa cacgctggag ccaatgcgat gggtgttctt
    13981 ttgacgggta tgggagccga tggcgcggac ggcctgctgg ccatgcggca aaataaagca
    14041 tataccattg cccaggatga agccacgtct gtcgtttacg ggatgcccgg agaggcggtt
    14101 aaactgggag cggcctgtga tgagttaccg ctggatgaaa ttcccggtcg gatcatcacg
    14161 cgtttatccg gcgaggataa aaaaagagaa gataaataac gatttaatct ttgggatttt
    14221 tttccagcca cttgtgaagc agtttgagtt ctttttcctt ccactgcggc gattgcctga
    14281 tgtgaagaaa agattcatag atggtatcga tggtttttac ggcattttcg atcagataga
    14341 ttctctccag caggctgcct gacgggtctt ccggcgtatc ctgccagtcg gtttgcggca
    14401 ttttcagcga ctggaaatca aacgaatcgg ctttgaacat aaaatcccat tcgttattgt
    14461 tatgaagcaa ttttattccc gcttccttga ctttcttccc ctggcggatt gcttccttgc
    14521 cttccttgag ttctgcgtga atgccgtgac agacgacacc ctgagagtat tcgccttcgc
    14581 cggcttccag cgaaactctt ttcagaaaat gaatttcaca ttcctctttg ttcggcagca
    14641 cgattctgcc gttacgttct tccgatttaa accacagcca ggtgagaaac tcgcggccga
    14701 tcaaggacgt cgattcggct ggatccgcat cctttctgat caggtgattt tcctgcgggg
    14761 aaaagcgttt cagattcaat gcaaacgttt ttttaaataa atccgcgaaa tcatcgataa
    14821 ccttgtcgga aagtgaagaa aaataaagtg tattttgttc caccgcccag caaacatcgt
    14881 aaaaaaaagg gatggcgtcg agtttggcca gcagatcaag tttaaccttg tctttcagag
    14941 ccgttgattg ttgccggaat attttattct tgccgctttc ggcaagaaaa cgcctttcct
    15001 cttccatcag tttgatcttc aagagcttcg gcgggatgag cttgcggtca atgcgcaagg
    15061 aaaaaatcag gtaggcgccc agtgaataat tggcattctt aaaatcggta tccagtatgt
    15121 cggttaaaga aacccatccc atggtttttt cttccgtcga cttgaatgaa tttttaaaag
    15181 aattggcccg gatccggttg ttgacaaaat ttaaaaaggc ctgcggtaac tggccatcga
    15241 cgctgaaacg cgagaaggta aaaccaccct tgagtaaacc catttttcaa tccccatttt
    15301 tgccgcagcc tatattatcc gggccggatg tgtcaagaag taatgactcc ggatttctct
    15361 taaatatcaa tcagtttata aatgcatccg cagactattt atccgtggac agaggttttt
    15421 ttttgtgtta gggaccttca tatttcaagg gtgaaatcaa tgaaaatcaa aaaaatcggc
    15481 attgccgcca atattgaaaa agaaaaaatt gcagaccacg tccgatcttt gaaaaagtgg
    15541 ctggaagaac gaggcgcaaa agtgtttctg gagacggaga tttccgggaa aattctttcg
    15601 gacggcggat acagctggag tgaactggca aggaaagcgg aagtggcggt ggttctgggc
    15661 ggcgacggta cgatactgcg ctcggcgcgt tatctggctg ttcaccgggt tcctattctg
    15721 ggcatcaata tgggcggcct cggctacctt acggaagtga acttaaatga aatccattct
    15781 accatggaat tgattctcaa gggaaaattt gtgaccgaaa agagaatgat gcttgatgtc
    15841 agtgtacaac acgggaaaac cgtggccggc attggcagtg ttttaaatga tgttgtcatg
    15901 aaccgcggcg atttatccag aatgaatgaa ctggaagtgg aagtggacgg ggaatatctg
    15961 acaacttaca agggagacgg cttgatcgtt tccacgccta ccgggtcaac cgcttattcg
    16021 ttgtcggccg gaggtcccat cgtatttccc ggaaaagatt tgatcctgat caatcccatt
    16081 tgcccccata ccctgacaaa ccgaccgatt attttttcag aggatgctca tcttcggatt
    16141 accctctggt ccaaagacaa aggcgcaatg ctgtcgctgg acggtcagga aacgcataaa
    16201 ataaaatccg gcgatgtcgt gatcgtgaaa aaatcaaggc atgccacgat gctgattctt
    16261 tccccatacc gcagctacgg agagatcctg cgttccaagc tcggctgggg ggatttacct
    16321 tcgggagcgc ataaaagaaa aaatgcttaa cgagttgagc attaaaaatt ttgccattat
    16381 cgacgaactg catgttacgt tcggtgaagg actgaacgtt ctctccggag aaacgggcgc
    16441 gggaaaatcc atcatcatcg gcgcggtgag tctgcttctg ggagacagag caacgtcgga
    16501 gatgatcaga acgcaggcgg ataccgcaac cgtcgaagcg gtttttgata tttcagccga
    16561 taagcccctg cgggacaaaa tcgacgccat gggttttcct gtttcggatg agttgattat
    16621 ccgccgggta atctcccata cgggcaaaaa taaagttttt atcaacggac aggcggcaac
    16681 cctggccaat ttggcggcca tcagcgaacg gttgatcaat atttgcgggc agcatgaaca
    16741 tcagatcatt ctggctgctg aaaatcatat tgatattctg gatgagtttg gcggctgcct
    16801 gtctgagcgc gcggtctaca cggaacattt caatcagtat cagcagatcc ggtctggaat
    16861 tgaaaaacta gaggagttga gcaaaagcgc agacgcaaga acggagttgt atgaatttca
    16921 gttgaaggaa attcaggata ttaatccgca accgaacgag gatacggtac tgtcggatga
    16981 aaaaaaggtt ctggtcaaca tccagaggct ttccgaatgg gcgggtaatg cctttgatct
    17041 gctttacggc gacaaaaact ccgtcatcgt catgttaaaa gaagttcagc gtcagattaa
    17101 agaaataaaa aaaatagacg ccggtctaaa aatctccgaa gccgagatgg aaaataattt
    17161 tgtcgcgctg caggaagcgg ctctgcttct gcgcgattac ggcaagggcc tttttttcga
    17221 tgccgaacgg cttgccgcca tcgaggagcg gcttgatctg atccatcgtt tgaaaagaaa
    17281 atacggcggt tccctggaag ccgtttttca aaaaaaacag gacatggagg aagccctccg
    17341 gaaggtctcc tcgctggatg aagagttgca gaaaatgatc cgggaagaag aagcggtgaa
    17401 aagcagactc cggcagagcg cgcggaatct gtccgaaagc agaaagaaag cggcgaagac
    17461 gctggaggac aaagtcggga aagaaatacg cgctttgaat atgccgcacg cctctttttt
    17521 tgttcatttt aaaaaccgtg ccgacgacaa cacggatttc ttcgggccta ggggagccga
    17581 cgatatcgaa tttgatcttt cggcaaacaa aggcgaagac gccagaccgc taaataaggt
    17641 ggcttcggga ggggaattgt ccagaattat tctggccctg aaaaatgttc tctccggcac
    17701 cgggtcggta ttgtccatgg ttttcgacga agtggacaac ggcattggcg gtgcggtggc
    17761 ggaaattgtc ggaagaaaat tgagagctgt gtccgccaat catcaggtca tctgcattac
    17821 gcatttgccg cagatcgcct gctttggaga taaacacatg ttcgtttcca aaaaagtggt
    17881 gaagggccga acgctcactt ttgtaaacga gcttgacagc gagcagaaaa tagaagaaat
    17941 cagcagaatg ctgggcggcg tcgatgtgac accgacaacg cgggagcatg cccgcgaaat
    18001 gctccaaaac gccggctcac ataacctcaa agtatcggga ggagacacat gctgagaaaa
    18061 gcgcgcatcg atgacgtcaa aatcattcac cgcctgatca atttatcgtc gggcaaaggg
    18121 gagatgctgc cgcgttctct gatggacatc tatggttctc tgcgggattt tttcgtctat
    18181 tatgatgagg atgaatcccg aattatcggc atatgcgcga tgaatattat ctgggcaaac
    18241 ctggcggaaa tccggtcgct gtatgttgag gagtcccacc ggggaaaagg cgtcggccgg
    18301 gtgcttgtgg aggcctgcat ctccgaggcc attacgctgg ggctcttcaa ggtctttacg
    18361 ctgacgtata aaaaagattt ttttctcaag cttggattta aagaaatcga ccggaacctg
    18421 ctgccggaga aaatctggtc ggattgcttc cgctgttcca aatacccgga ttactgtgat
    18481 gaagtggcga tgattgtgga actatgagat attcactgaa aatcctgctg ctaatcttga
    18541 tggctttctt tctcggcggt tgcatgcgtg ccgtttccca accgtctttc aaaacgctgg
    18601 actcgctgat tcctgtggat gccacgcagg ttgctttcag tgatgatttg gagcctgcat
    18661 cgctagaaac agcgattgac cgcagcatcc gtttttatga gggccgcgga cgcaataacg
    18721 tttatcagat cgaagacagg cagattgacg cgcagcagtt caaggaaacg cttctcgctt
    18781 ttcgcgccct gttaggcgat gcgaaggatc cggagacatt gggaaaaagg atggccgaag
    18841 cattcgatgt ttatcaggtc accgggacgg atgatgaaag ccgtgtgctt tttaccggtt
    18901 attacgaacc gctcctggaa gggtctctgc aaaaaacaga gaaatacaaa tatccgctgt
    18961 atcgggtacc gcctgatctg gtgagaaagg gaacccggat cggacggatg cagaacgaca
    19021 aatttgttcc ctattacagc cgccgggaaa ttgatgtcga cggtgttctc cggggcaaaa
    19081 accttgaact cctctgggtt tcggatccgg tggatttatt ttctctgcac atccaggggt
    19141 ccggcaaaat caaactggaa aacggggacc ttctgacggt cggttttgcc aataccaacg
    19201 gccggccttt ccgcagcatt acgaagttcc tgctggacaa aggcacgata cagcatcatg
    19261 aagtcacgta ccgtcatgat tttttacggg ggaaacgtga cgaggaaatt tatgaggctt
    19321 taagccataa cgaacggtac acattttttc gctttctgga aaaagatccc gttggatctc
    19381 tgggcgaaac ggtaacaccg gaccgaacga ttgcgaccga tccggaatat ttcccggaag
    19441 gagccctggc cttcatccgt ttgcgcaagc ctgttttcaa tacggatgga catgtcaagg
    19501 agagggtcga attttcccgc tttgtgctca gtcaggacaa aggcgccgcc atcaagggac
    19561 ccggtagggt ggatttgttt tgcggattcg gtgaaaaagc ggaaaagacg gccggcacgc
    19621 tgaaggaaag aggcgaactc tacttgctgc tgaagaaata aatccgtttt tccagcgcat
    19681 ggccgctaga gctgttccac gtcagggagg ggcttgcacg acgcaaaggc agaagcttct
    19741 tgtttcgtcc ggattcatgg cgatccttga gaaaagctac gaatgatttt gggttgtttc
    19801 ccggatataa catcggggat cttcttgcag ataatctccg ctgtaggcat gcgcccgtcc
    19861 tcggcagccg ccgcagacgg ttttgtaatg gcattcgccg caggcgcctt ttaaaagctc
    19921 ttcccggcgc cgcaaattct gaaatacggg cgactcttcg taaatggcct taaaatttct
    19981 gtcacggata ttgccggcgt taacctctac gaaaggacag ggccagacat cgccgttggc
    20041 tttgacgtac acaaaaccgc gtccggctgc gcaaccgtga aacactttac cggcaagttt
    20101 cagccggagt ccttcggtga atcccttttt ttccagcagg tacggccaat actgaggacc
    20161 ggcaaccggt tcaataatgg ttgttgcgtt tttctgtttt tcagcgatca tttcacttaa
    20221 atgtttgttg gcacttttct ttaacgtggc tttttcgatt ttttcacccc ggcccacggc
    20281 gaccagttgg tacatcagca tgatgccggc accgcaacgg tctgcaaaat cgatcagttc
    20341 gggaaggtgc ggcatattgt attccatagc cgtcgtgttg atctgcaaaa ggatgccggc
    20401 cttctttgtg gctttaatag cgcgaagcgt ccggtcgaag gcaccgggtt tattgcggac
    20461 catgtcgtga atggcagggg tagcagcatc caggctgatc gcatgacaga ccacaccgtg
    20521 atctctgagt ttaaaagcca tttcttcgtc gatcagcgtg ccgttcgtgg cgatgatgtt
    20581 ggcgagaccg gcctgctgcg agtgccggag cagctcgaaa atatcgggtc tcaccagcgg
    20641 ttcgccgcct gtataaacca gggtgcggaa tgcacttaca gaggcaatgc tgtcaatcag
    20701 tcttttgcct tcgtcggtgg acaattcatc tttgcccgcc ttatcgctta ccgcatgaca
    20761 atggatacat ttcagattac acgcgccggt gacttcccat acgggatgtc ccggataacc
    20821 tatgcagccc atccccaagg cgccgttggc caccgggatg gaccggaaac cgtattgaaa
    20881 gccccagcga ggatagtttc tccaattatt gagataagcc atcaacagag aactgcccag
    20941 ttgcttgagc agtaacgagg gaggattata aaatgctttg accttctttt taacgttcgt
    21001 catttcgctt aaatatatgc cagaatataa accagagaag ttcccaggtt aaccgctata
    21061 ttcagaccgc acaatagctc catggttttt ctgttttcgt attgatcacg taacatcatt
    21121 acggcaataa ataaagatac aatcgctaca ggcagataga gataaaccgc tttgaacggc
    21181 actccgaaaa cgggagagga aaacacggcc gtcgatgcca ggagagaaaa aacgaagaag
    21241 agtactttcc ccttgcgttt ccccaggcga taaagaaggt ttttcttgcc ggtggccttg
    21301 tccgcttcgt aatccgggta ctcgttgagg aagatcacgt tgaagatcgt caggccgatg
    21361 gggacggtga tccagtgaat gaccgaatga acgaagccgg tttgtatata ataggcagag
    21421 gcaaccggaa gccatccgta acagaaaccg ataaaaattt ccccaacgcc tttctgcacc
    21481 agcctgaccg gttgggtgga atagaagaac cctggaaaag cgcccagaca gcctaaaagc
    21541 agggtatagg ggcctgtctt caggataaat tgaagagtca gtccgatgat cccggcgata
    21601 ataaaggaga tcaatccggt ccgtaaagcc accgaacgtt tcactgtttt tgccggaacg
    21661 acgcctgatc cgcctgcaaa cctgctttta tacaaccgct gagatatttc atcttcccgg
    21721 taatcaaagt attctcccat gtgataggta gaaagcatga tcaggataac cgccgaaaca
    21781 cccagcgcaa acacagccgg gtcgaagaca aggttgatct tatatgccag aatcgttccc
    21841 agaataaacg ggaggatacc gacggtgtga aacgccggac gggagagtga aaaccaggac
    21901 tttatttttt cgaaatggac gctctgcata gacccccgca aaaaataagg tgcaggtctt
    21961 aacataacag gtaccttttg taaagcatgg cagccgattg ccgacttcca tgggacactg
    22021 gcggtgcagg cagcacgacg atcaggatgc catcgccgca gcacacacgg acgggaaaaa
    22081 gcattaaaaa aacatttttt tcttgattgt taatctttat tgtgctagtc gtagcgcgag
    22141 cttgaggggg atggctcctt tctaaaccag agaacaagcc ggtttgagta acgttagttt
    22201 gcaaaataaa gaataacgaa gcatttttcg tgatgtttgc cagagcgatt tatctgtttc
    22261 aaatggtttt ggttatggcc gcgaaggcgg ttgataataa aattcaaaat ttgattttac
    22321 gaaaggggga ttttagaaga tgacgaaggc agaattaatc ggggttatag cgaaatcagc
    22381 aggcattacc aaggataaag caggcaaggc tctggatact ttccaggatg cggtaacgaa
    22441 agagctcaag aaaagcggca aactggctct ggtcggcttt ggcacgttct cggtggttaa
    22501 aagaaaagcc agagagggca ggaatcctca gaccggcaaa acgatcaaga ttcccgcgaa
    22561 gaaagtcgtt aagttcaagg caggcaaaga attggccggt aaagttaaat aacaaatcaa
    22621 ttggttcttt aaaactaagg cctccgtatt ctgtcatagc cggaggcctt agttcgttag
    22681 tgagacccga atttttaaaa acgaagcatt gaaatttgtc agttgccatg agacccaacc
    22741 ttcacaaacg tcgttaacgg aagcttgctg gtcgaatgat tccacagcca taagccgtgg
    22801 aaaactatcc cgcgagcgac gctcggcggg acagtacggc gaaacaggaa tcgacggcct
    22861 gtgatgccga gtaacatatg caaaaggagt tgttttggag cgtaagagga tactcagcgg
    22921 gatgaggccc accgggaagc tgcatctggg aaaccttcac ggcgcattgc gcaattggat
    22981 tgcattgcag gaaagcggcc aatacgactg tttttatttc gtggccgact ggcatgcgat
    23041 caccagcgaa tacagctcca cggaaggcat tcaagacaac gtcatcaaca tggtcatcga
    23101 ctggctcagt gtcggccttg atcctcaaaa gagcactctg tttctgcagt ccgcggtcaa
    23161 agaacacgcg gagctttttc tgctgctttc tatgattacg ccgctggcct ggctggaacg
    23221 caatcccacc tacaaggaaa tgaaaaccga gctggccaat aaggatttat cgacgttcgg
    23281 tttcctgggt tatccggtgc tgcaggctgc cgacattatc atgtacaaag cctatggcgt
    23341 tccggtaggc gttgatcaac ttccccatgt cgaattgacc cgggaaatag cacgccggtt
    23401 taatttcctt taccgggaaa tatttcctgt cccggaaccg attttaacga atgttccgaa
    23461 gctgctgggg accgacggca gaaagatgag caaatcctac gacaactcca tttacatcag
    23521 cgatcgcggt aaaaatctgc agcataaaat cagctccatg tttaccgatc ctcagcgcat
    23581 gagaaaaaaa gatccgggta atcctgacat atgcaatgtt tttactttcc atggattata
    23641 ttcttcaccg gagaaggtga aagagatcga tgtcgaatgc cgcagagcgg ggatagggtg
    23701 tacggactgc aagaaaatgc tggcccagcg cgttgccgaa aatctgcaac ccgttcacga
    23761 gcgcatggat tattatgtaa gtcacatgga tgaaatcaac ggagttattg aagaaggcaa
    23821 tcgaaaagcg gcaaaaatcg cccgtcagac gatgagtgaa gtccgggccg caatgaagat
    23881 ttgaatttcc gtcatggata ataataattt aggtcatccc atggatccta tggacaatga
    23941 gcagcgtttg aattatgcca tcaagctgga tatctttgaa ggtccgctgg atctgttgct
    24001 ttatctgatc aaaaaaaatg aaattgacat ttacaacatc cccattgcgc tgattaccga
    24061 gcagtatttc cagtacctga aaatgatcaa agcgctgaat ctggacctgg ccggagaata
    24121 tctggtgatg gcctccacgc ttattcatat caaatccaga acgcttttac cgccgcctga
    24181 agaacccgaa gaggaagagg aagatccgcg tgccgaactg gtcagacagc tcctggaaca
    24241 taaaaccttc aaggagattg cggtcagtct gggaaaccgg cctcttctgg aaagagatgt
    24301 cttcacccgt gcggcagctc tccctgaagt ggaggtcaaa tcaacggatg aagaggaaga
    24361 gtttattgag gtcagcgttt ttgaactgat cgaggccttc catcgtatta tctctcagat
    24421 ggataagaaa gaattgatgg aaattgatct ggaaaaactg tcgctgaccg atatcatcaa
    24481 cgaaatcatg gaacagttga cggtgaccaa aaacatcacg tttgaagatc tgcttaaggg
    24541 gaaaaaagac cgccgtcgca ttatctatac atttttagcc atcctggaat tgatcaaact
    24601 cagaatgatt aaagcctatc aaacttcaat gtttggtgtt atccggatat ttccggctgt
    24661 ggagtcgtaa atggaaaaga ttaaagccgt catcgaagct ctgatttttg cttccgacac
    24721 cccgttgtca cctgaaagaa ttcgcgccgt ttttccggag gtggaaaaaa cagagatacg
    24781 ggaaatcatt gatcaactgg tctcggaata ccatgaccgt caggggggaa ttctcctgca
    24841 ggaagttgcg ggcggttttc aattccgcac caatcccgat ctcagcctgt ggatcaagaa
    24901 gctcaaaacc gccaaaccgc agtctctgtc cccgcaggcg ttagagaccc tggccatcat
    24961 cgcttataag caaccgattg tgaaatcgga aattgaagac atacgcggag tggatgtggg
    25021 aggtccgctc aaaggtctgc tggagaagaa acttgttcgt attgtgggcc gtaaagatgt
    25081 gcccggaaaa ccgattattt acgggacaac ccggaaattt ctggaggtct ttaacctcaa
    25141 agacattatg gatttgccca atattcgcga gttaaaggaa cttcatcagc agcaggaact
    25201 tcttgatccg gatcaggaaa gcagcgagtc cgaacaggaa accatcgtag aagaagaggc
    25261 tgtcgcaaaa cagcatgacg atgaaaatgc agaagaaaat gcagaagaaa agatagaaga
    25321 agttcctgat ttatgaaaga gcgcttgcag aaagttattg cagcggccgg cattacctcg
    25381 cggcgtcacg ctgaagagtt aatcacccgg ggacgggtca gcgtcaacgg tgttgtcgta
    25441 acgaaactgg gagaaaaagc ggatgctcag aaagatatta ttcgcgttga cggcaagatc
    25501 atctccacgg aaaaagtaaa atattatatt gttttaaata aaccggccgg atttgtaaca
    25561 acccttcatg atcctcagaa caggcccacg gttgtggatt tgctaggcag tgtgccggag
    25621 agggtttatc cggtgggaag gttggattac gattccaaag gtctgttgct tctgacgaat
    25681 gacggcgatt ttgcccagaa aattcagcac ccccgttttc agaaaccaaa aatttacaaa
    25741 gtcaaaatcc agggacattt atccagacaa gatgtccagc aactgagcag gggcattaac
    25801 gttgacgatg attttttcaa accggaaaat ctcaaagtgg ataaaatgaa cgacaggagc
    25861 tgctggttgc gtcttacgct caaagaaggg aaaaacagaa ttatccggag agggtttgaa
    25921 aaaatcggtt acagagtcgc cctgcttgtg cgcgaggcga ttggtgatct tgcgttgggt
    25981 accttgaaag agggcgagtg gcgccatctg acccggaagg aaatcagtca attgctggac
    26041 agtgcagtca agtccaaata gcaaaatttc cttgactttc gccaaaaaat aaataataaa
    26101 cacacgcata gagaatatca aaatacagtg tcatctggct agtatgagtt gccatcagtt
    26161 taaggaggag atatgaacaa atccgatctt attgaagctt taagcacgaa gttaaatctg
    26221 acggaaaaaa aggcaattga tgtcgtgaat ctcattttta agggttttac cgatgagtta
    26281 aagaaaaacg gccgtattga aatcagagga tttggcagtt ttgttgtgaa aaaatacgga
    26341 tcatataccg gaagaaatcc gaaaaccgga tcgaaaattc aggtcgaacc taaaaaattg
    26401 cccttcttta aagtcgggaa agagcttaaa gtgagagtcg ataaaaagaa ataatcattc
    26461 ctgtctgttt ttatcctata aaaaaggagg catttttaaa gcctcctttt attgttttaa
    26521 cgattgaaga cgccctgcct ataggttggc attaacgaaa tcccagttaa tcagattctg
    26581 aatcacggcg gaaagataat ccggccgcct attctggtaa tccagatagt aagcatgttc
    26641 ccagacatcg atggtcaaaa gagctttctg accatgcgca aggggcgtgt cggcattggc
    26701 ggttttcgtt atctgcaacc gggttttgtc cgaaacaagc cagacccatc cgctgccgaa
    26761 ctgtgtggcg cctgcatttt tcagctcttc aacaaattta tcgtaattcc cccacatttc
    26821 attaatttta gccgcgatgg cgccgctggg acggccgccc cccgttttct tcagacattt
    26881 ccagtagaag gtgtgattcc agacctgagc ggcattattg aaaatgccgg cctttgcgga
    26941 atctttagcg gcaatgttga tgatttcttc cagcgatttt tgatcaagat ccgttcccgc
    27001 aatgagcttg tttaagttgt ccacgtaagc cttatggtgt ttaccataat gaaaatccag
    27061 ggtattggcg ctgatcacgg gcgccaacgc atcttttgcg tagggcaatt ccggcaatgt
    27121 aatggccata attattctcc ttttttgaga gttgattttt accttcttgc tgcattcaag
    27181 agaagttgaa aaatacttaa cagacttatt ccgattacaa agagcatcgg aatacactga
    27241 acacgtgttc gtattttata ataggtcgtg ttttaaattt gtcaaattga tatttgaggt
    27301 ttttcacaca tcgccgcaat cgtcttctat gcgtcttgcc ttgaaactga gccagttcat
    27361 gtaaataaac gtaaatacgg caaaaacaag cggtggcacg gcgacttccg gattaaaaag
    27421 ggtcagggct atcccgccgg ccagtccgta atctttcatg gttccgagca ggatgaaaaa
    27481 attcaccttg ttttcctgac cgtgaaagta gagaccgatt cttctaatga gaaaataaaa
    27541 taaaaaagtg ctggttaaag cgatcaggga tataaacaca agatcgtctg aaagcttttt
    27601 taaaaacatg ctgctgtttg cggaaatggc ataaaaaacg ataaaatgac agaagtcgat
    27661 cagcgtattt tcatgtttcc tgagaatccg gtcccaatct ttctctacaa tcagccggga
    27721 aagaaccagc ggcagggcga tcagacacag gataagcacg gcaatgttcc agtagtttat
    27781 ctgtatatat ttgaaaaagc ataagctgac taaaggcacg atcagcaggg cacccagata
    27841 ggttcccgat attgatgtaa agacataatt cttctcaata cgcagtaata ttcccagaag
    27901 aataatttcc agcgatgcag gcatggcagc gaccagcgcc aggccgatcc agagttcctg
    27961 tttttgaatc agaaaggcac cggttagaag gatgaaattt cccaaaacga gataattcaa
    28021 aaggttgccc cggatggagg aaaacaaaag agaagcggga tgacggaaaa aaccgcgtgg
    28081 aaatctcagt aacgtaatgg tcagaataat catcagggct ggcagaataa gtattcttcc
    28141 cgcctgacgc ccctcgtcga aaaccatacc gcaaaacagc gcgataatga aaatgatgct
    28201 tgaacggtat gcctgaaagg gaagggtcat tcggcgtcac ctcccgaaag ccattaattt
    28261 tctggcgaaa aaaacacata aaagaaaaaa tatgtttaag accacaatgg ttcctccggc
    28321 cggtaaattc aacaaaaagg ccatcgtaat tcccaatatg accgataata ctgagaaaat
    28381 aattgacgta aaaatggtca ttctgaaact taaagacaat tgcagggcgg ttagcggggg
    28441 gataatcaac atggccgaaa cgagcataat accggcgact ttcatggcta aaaccgccgc
    28501 aaccgctgtg agcagaaaga gaaccgtatt aatttttccg gtttgaacgc ccatggttcg
    28561 ggccagttct tcatcgaatg tgatggccag aagatcatcg tacaggatga tcactgtcgc
    28621 cacgacggtc agaaaaacga tgatggcaaa aaagatttcc gttagggtta cggtcagaat
    28681 gttgccgaac agatagctga aaagatcaag gttgtatccg ccggagatac tggtcaggat
    28741 aatgcctgca gcaataccga aggaagaaat gattccgatg gccgcgtcgc cctgaatccg
    28801 ttttgaacct gtcagtttca gaatggccag cgacgacagc atcaccagcg ggagcgccgc
    28861 cagcgtcata tgcgcgggac tgatacccag cagcatgacg acggcgacac ttccgaacgt
    28921 gacgtgtgaa agaccgtcgc cgatcaggga aaagcggcgc agaacaagaa atacgcccag
    28981 gatggaagct atcgccgcaa tgagtatgcc tcccagaaag gctctctgaa cgaatgcata
    29041 ctgaaaaatg ccgctcagtt ccatggcgtt gcgggtcctt tctgatcgtg ttgatggcaa
    29101 atcatgtgct ggctgttcgg tccgaaaaat cccgtcatat ccggcgatgc gcaaaagtcc
    29161 ctgaaggttc cgaaaaagat tattttcttg tcgaggtaga gaagattgcg cgcatatttt
    29221 ccaatcgtcc ctgtgtcatg agtaacaaga acaaccgtcg ttccatgctc attggccttc
    29281 gcggtcaggg cgtagaaaac atctctcgtt tccggatcca aagcggttgt cggttcatca
    29341 aaaatcagca aatccggttt tctgatcaaa gcccgggcga gcatgacccg ttgctgttca
    29401 ccgtaggata gttctccaat gaggcaggag gaaagatgcg cgatacccat cagatcaagg
    29461 gttcttttaa gatcatccgg attccttttt ttgatgccga gttgtacgat ttccgcgacc
    29521 gtgcagggga aatggtaatg cagcgcgttg attctttgcg gcaggtaacc gattctgtgc
    29581 cactgatcaa aggatgcaac gggctgatca aagattgtta tatgtccctg gtgaggttca
    29641 aggatgccca gtatattttt gatcagagtg cttttgccgg atccgttgga accggcgatg
    29701 cccagatagt ctcctctctg gacggtaaag gaaatatcgt tgattacttt tgtcccgttg
    29761 taacagaacg agagatgatc gaccttgatt atttctccgg acattgtaat cctttttgaa
    29821 aattcacgag atttttttcc attaaagcaa gaaaggtttc tcctttttcc agatccgttc
    29881 tggtgacatc gtgtccgtta ttgagtttca gcagaccggt gcccgtttct cgggcaatgg
    29941 tttttgccag acgcggattc atcatgtctt cataatagac gtgggatacg ttttcatttt
    30001 tgatctgatc gattaaggca aagattttct gcggtgccgg ttctgcatca gcgaaaacat
    30061 tataggccgc aatgtatttc agatgatatc gtgacgtcag ataggcaaat gcccagtgac
    30121 ccgcatgcag aaccgttctc gttttacagg cggccagtgt tgtccggtat ttttgatcca
    30181 tgtccgccaa ctggcgtttg tagtcaccgg catttttcaa gtaatagtca ctgttgcgag
    30241 gatctttttt aataaacgca ctcgttatat tgtccaccat tttttgagcg ttatccatat
    30301 ccagccagat atgcggatca aaaccggcag gatgtgcctc atgatcgtct tcttccttgt
    30361 tcagcctcag catcatggcg ccgttcccgg tttccaccgc ctgcagacgg gtgtttttat
    30421 cggaggcgcc gatgatttta taggcccact gctccaattc aaaattcgta aagagaaaaa
    30481 catccgcgtt ggtgactttg atgatatcat ccggcttcag ttcgtaatga tgcgcgtctg
    30541 ttcccggagg caagagcatg gcgagggtga ttttatcacc gccgatatgg cgcgcaaaat
    30601 cgtaaacggg aaaaattgtg gcaataacat tgagtttctt gtcattccgg ccggggtctt
    30661 tggcctgctg gcaggaaaag attaaaacca aacaaagcag gatcaacagc ggggttcttt
    30721 ttaaaagcat catcaatctt ctttctcagg gcagggttga tcatagcaaa taaattatgt
    30781 cgtgtccttt catcaaacac gcgacgaatg aaatctgacg tttttatatg cggttttttg
    30841 ccgggacgtc aaattctttc gtatccagcc tggtttttga aaattatcag aaatgcaatg
    30901 gtgatgcaac atatattgcc ccatgaataa gaaggcaatg aaaaaaatcc tcctctatgg
    30961 tatatagaaa catatcatgt tcaaaaaaag gaggcagacc tatgaaaccc atcggacctt
    31021 tgatgtggga acaccggtta atcgaaaaaa tgctgactgc aatgatgcgc catatggatc
    31081 aggtggaaaa gacccggaag gtgaatccgc tggttattga tatggccgtt gattttatcc
    31141 gtgtctatgc cgaccgaacg catcacggca aggaagaaga gattcttttc cgggatctgg
    31201 caaaaaaaga tcttcctgcc gatttgaaga acatcatgca ggaactgctt gacgaacatg
    31261 tctggggcag aaagacaacg gctgcgctgg tggaggcaaa aaataaatat ttgcagggca
    31321 atcaggccga attattccgc ctgattgata cggcaagaca actgggcaat ttttatccca
    31381 agcatatcga aaaggaagac aaacacttct tttatccctg tcaggaatac ttcaacaagg
    31441 aagagcaaac caagatgctt gacgaattct gggaattcga ccgcaaaatg attcacgaaa
    31501 aatataataa aatttatgac gactattccg ggctttagcg gctaataatt ccacccccag
    31561 aatccaaatg ccagtacgtt cataataatc ggcgcggcgt agatgatgcc aacggtcgtc
    31621 agtttggcct ttatggattt caggctgatg accagaatgg caaagcagaa aaagtggaaa
    31681 tagccgatca gaaaaatgag ccagacgcct ttaaaggaca gataccagaa aggatattcc
    31741 cagaccagat ggcctccgat gttgagcagg cattcgacaa aaacgcaaaa gatgctgtag
    31801 ctgattgccc agaaccattt ttcaggcagc cccaggattt tatctttttt gctttcggat
    31861 aacgtatgat agaaaataat tccgatgatt aagaacatga acataatttc gatgttccat
    31921 ccgaccagtg tccgcaaggc ggtatcgccg ggggttgtcc agaaagcgga gcgctgcgtc
    31981 aggtacatga cccagccgtt ccatgtttcg ttgaagaaat ccacgccgaa aatagtcagg
    32041 ccggcgagaa ccgcattcca gtctccgttt tgtcgtgcat ctttgatttc cttggtgtaa
    32101 atgtaaaaga cgatagccaa aagaggaatt acataccatt tgatcatgga aagatctctc
    32161 aggccggtta aagcttgctg ggttgcttct gtcataatgc cctccttaaa ttttttgagt
    32221 taaaaaatcg gttaaggttg tcaatgcttc ccatctttta ctttgctaaa cttttataat
    32281 tatattatat atctttcaaa aataaaaaga aaaaagtcag ggtttatgat cctgattgat
    32341 cataatgaag ggagtgttta tgaatcttaa gccgatatat cgtcttttcg ccgtcgtaat
    32401 gattttttct gttttttgtc tatccggctg cgccggaccc agactctaca gtgtcggtat
    32461 gtattatgac gcagagcaat cggtgatccc cgcgtatgtg aaaaatgacg ggcagacatc
    32521 cgacgctgtt gtggccgttt ccgaatttgc cgatacacgt cagattgacg acccgctggt
    32581 catcggccgc gtgattcaaa acagcggcga aaaaaccctg gtgttccccc gcaatgtcag
    32641 agcaacgagg gctgttgcga acgggataaa gcaatatttg aaaaaagccg ggtataaaat
    32701 taccgaaatc aatgaaccgt ggaatttgaa agaagataaa atgccgatgg gcaaggccaa
    32761 aattctgatc ggtggtaata tagaagaact ggaaatcatc tgccggaaag gaaaactgac
    32821 gaattcctac gatacgacga tgacgctggc cgtcgttatc gcggatatgg gccaaggcag
    32881 gatactgtac aaaacgcggg tgaagagttc ttacagccgg gaacatattt tgttttccga
    32941 aaatattctc ggacagcagg cggacaaggt tctgtccgat accattgaaa agctttttga
    33001 ggaccaaaag gtagcgcaga ccattaagga tgtcatggtc cgttaatgga cattaaaggg
    33061 ccgcctgctc ctgcggttta atgatgactt tgatggattc cctgccgtcg gcaaccagtt
    33121 gaaagccctt tacgatctcc gtcagcggca accggtgcgt gatcagatca tcgatggcga
    33181 catgatgcca ctctatcagt ttcattgctt caataatgtc agaaggaccg cagtaatacg
    33241 acgtcaggat ggcaatttcc ttcatccaga agtcgttgac cggtacaacc acttttttat
    33301 ccggtgcggg gacggcgaac aaaacgatga cgccgccttt gtcaacgcat tgccatgcct
    33361 gttccaccgc cgcatcggcg gaagcacaca gaaaaacaat atcggccttt ctgccggtct
    33421 gatctaccaa ccgttttgaa atatcatcgg ctgcgttgat agcaatatgg gctcccattt
    33481 ccagggcgat atcgagtttc ttgcggttaa tgtcggaggc gatgatccgg cagcctttgg
    33541 tgacggccag ttttacgttg agcaggccgg acatgccgca gccgattacc agaaccgtct
    33601 gtcctttttt tacgcgggcc agccgttgcg ctctgacaac gcaggccagc ggctcaatga
    33661 aggtgctttg gtcataatca atgctgtcgg gcagcagata tgttccattt tttattaaaa
    33721 tctccggaac aaggatgtat tcggcaaaac cgccgggcag cctttctttg atctcggcac
    33781 actgcgggta gtgcccgttg cggcagtaat gacaggtcat gcagggtact ttcggggcga
    33841 taaaaacgcg gtcgccgggt ttatattttg taatggactt accggtgctg acaacttgcg
    33901 cgcctatttc gtgtccctga atgagcggcg ctcgcggcag ccggtaccat tcgacaatgt
    33961 cgctgccaca aatgccgcag gacatgacct tcaccagcat ttccttcgcc cccggtttag
    34021 gcgtcggaat gtcttcaatg cggatatctt gattgttgta ccagtacgcg actttcatgt
    34081 tctttatgat catcgttttt gttggttgtc gtgaaactca aactttaaaa gtgcaacgac
    34141 tgaaagtttg cgggtcgaat tatcccaccc cgataatatc gggatgggac attagcccgc
    34201 gagcatcaag ctcgcgggag tttgcttaat gttttatttt tttatctttt caaactcttg
    34261 tagagatcga aagctttttt tggcgtttcg ttgtcatgga caacggcgcg caccgcctgg
    34321 atcatggcaa tcggcgcatc cgactggaag atattgcgtc ccatgtccac accggcggct
    34381 cctttttgca cggcgttgta agccatggtc agggcttcca gttccggaat ctttttgcct
    34441 ccggccatga caatcggcac cgggcaggaa gacgttacgg tttcaaaatc tttttccacg
    34501 taataggttt tgatatagtg cgcaccgagt tccgcgcaga tccggcaggc cagccggaag
    34561 tatttggcgt cgcgggccat atccttgccg acggccgtca ctgcgagcgt cggaatgccg
    34621 taccggttgc cgatatcgac catcttggtc atgttgatga tggactgcct ttcgaattcg
    34681 ccgccgatga aaacctgcac tgccatagcg cagacattca gacggatgga atcttcgata
    34741 tccacagcga tttcttcatt agagagctcc ttgagaatgc tggcgccgcc ggaaacccgc
    34801 agaaccacgg cctgggttaa cgacggcggg ataatactgc gcaggatgcc ccgggtcagc
    34861 atgagcgtgt ccgcgtaagg cgccagggga agaatgttga tatccacccg ttccagtccg
    34921 gtggtcggtc cctggaaata accgtggtca atggccagca tgactgtgcg gcctgatttc
    34981 ggattgaata tccgggaaag tctgtttttc attccccaat ccagcgagct cgatcccttg
    35041 agaaaaaaac cctgtgtttc ctgaggcatg tcgcaatgaa acattttggc ttctttgatt
    35101 ccgtccgcat ccggcatatt tgctttctcc tttcccgtga tgaatcgttg tccagaaatt
    35161 cgtgaagacg tttttgttag acgcaatctt gcacattttt tttaattcgt caatttgaca
    35221 ctggcggcag acaatccatg tccggcagtg agtcggagga tgcttctgaa taaaacggcg
    35281 attgtccgcc ctgggtgggc acagcaggac ttaaacgcac tcctcctcca gtggaaaagt
    35341 aaacgccctc aaaccggcgc gcggatgctc aaccttcagg tttctgacga gttcacaaag
    35401 cggttttatt ttgtcgtcac tgacggccag catcagggcg ttattattcc cgggccagta
    35461 atgcgtgccc agtttcggtt cggtttcttc tccttcgccc atcaatccac tgatcttggt
    35521 gtatgccctg aatcctgttt ttttaaaggc ggcaataata tcgtcatccg ccgcttccgc
    35581 ataaatcacg tagaacattt tcatgatcgt cacctcttgg tggaaaaggt atttggaaac
    35641 attgatcaat caatcgtttt ttttgctcgt cgtttcttaa aatactcctg ccagtcatcg
    35701 aatatgtcgt aggcagcggg cacgacaacc agcgtcagaa tcagagacgt caaaaggccg
    35761 ccgataaccg cgatgcccat cggagcgcgg gtttccgcgc cttccccaac agcaaacgcg
    35821 atcggcagca tgccgaaaat catggcaaag gtggtcatga gaatcggccg cattcgtacc
    35881 ggtccggcct gcaagatggc gtcgcggcgc gacatgcccc gttcccgcag aacattggtg
    35941 taatccacca gcaaaatggc atttttcttc accagtccca tcagaagaat cagaccgatg
    36001 aggctgaaga tattgagcgt ttttcccgtg agaaacaaag cgccgaatgc tccgatgaat
    36061 gacagcggca tggaaagcaa aaccgttgcc ggatgaatga agctttcaaa ttgtgaagcc
    36121 agaatcatgt acgccatgat aatacccaga atcagggcaa agagcaggaa gatgaaagat
    36181 tcctgcataa cctcggccat gccgtaatac ttcggcacgt aatccgccgg cagaatccgt
    36241 ccggcgattt catccagctc gtttcgggct tcgcccagtg gttttccttc cagagaggca
    36301 aaaatggtga ttgcccgctg acggtccgcg cggttaatga cgctggggcc cgtgccttcg
    36361 gcgattctca ccacattgct cagttccacc agctttccgt cgcgggcgcg cacataaatc
    36421 ttctgcatgg cttcggaact ttcccgatcc tcggcgttga gccttacccg gatatcatag
    36481 cgtttgccct tcttctcgtc cttatacctg gcgatgtcca gttctccgcc caccagcaaa
    36541 ttgatggctt cagcgatcgt ggccacatcc acacccagat ccgccgcctt gtcgcggtcg
    36601 atataaacct tgaattccgg tttccccgct tccagggagg tatccacgtc cacaattccc
    36661 ggtcgtttgg cgaattccgt ggtgatctgg cgggcatatt tctgcaattc atttaaatcc
    36721 tgtccgcgga tgctgtactg aatcggcacc tgtctgatgc cgccgccgac cagcgaaaca
    36781 tcttccgccg acaccttcag tccgggaatt tgtttgaggt tcatgcggac aatttttttg
    36841 atatcttcct ggcttttttc acggtccgcc ttgggaatta acccgacgat cataatgccg
    36901 cggttggcgt atccctgata gccctgaacg tagaagaccg atttaatctc cggaatgtcg
    36961 cggaccattt tttcagccgg cctgaagtac ttttccacct gttccacgga ataatcgatg
    37021 ggcgcctcca ggcgaatgat aaaattgccc tggtcttcgg gcggcagaaa ttccttgccg
    37081 aggccgaata tcataatgaa aatgctgaac atgaaaacca tcagcgttgc aaaaagcatt
    37141 gccgccctgt gccggagcga aaatttcagc acgcggccgt agaacgtttc cagtcgctta
    37201 taattttttt ccagaaaatc gccggctttt ttccaaaagg accagcgggt gatgacgttg
    37261 cctttcgggg gcggggcatt gtgcggtttg agaaaaatcg aggccatcat cggcgtcagg
    37321 gtaaaggaaa cgagcaggga taccagaacg gcaaagacga tagtcagggc gaattgcagg
    37381 aaaaaccggc cgatcaggcc tttcatgaag gccaccggca ggaaaatggc gacaatcgcc
    37441 agggtcgtgg ccatgacggc cagaccgatt tccgatgtgg cgaatttggc tgcttccacg
    37501 ggacccattc cttcttccac atgccggtaa atattttcga tgacaataat ggcgtcatcg
    37561 atcagaagac ccactgaaag cgacagcgcc agcatcgtca tattgttgaa ggtaaagccg
    37621 aatgcgttga ttaacgcaaa agtggaaatc acggagaccg gcagggcaac ggcgctaatc
    37681 agggttgtgc gtatgttctt gagaaaaaga aaaacggcca gaacggcaaa gagactgccg
    37741 agaattaaat gcttctgcac ttcatcgatg gatcggacga taaaaacgga ttgatccatg
    37801 gcaatgttga tcttcatgcc gggcggcaat gtttgattga tcctttccac ttctttcttg
    37861 acccgcgcag cgacggccac cgtattggtt cccgattgtt tctggattcc gattcccacg
    37921 gccgggatgc cgttaaaacg cgccagcgat cttttttcct ccgtaccgtc ttccgctctg
    37981 ccgatatctt ttattttcac cggcgcgcct ttgtaataac tgacgatcag atcgttaaag
    38041 tccggaatgc tgggaaactc gcctttgatc cggacggtat attccttggt ctgtgtctcg
    38101 atccggccgc ccggcagttc gacattttca cgtttcaggg cgacaacgac gtcggagggc
    38161 gaaacttcgt acgcccgcat ttttgcggca tccagccaga tgcgcacctg tctgagccgc
    38221 aacccgttaa aagtcacgtc accgacctca ttgatccgct gcagttgttc cttcaatact
    38281 tcatcgacgt aggtggaaag atcgcgtatt gacttctcgc ccgtcaggtt catgtataaa
    38341 atcggctggg agtcgggatc gactttggca atgcgcggtt cttcgatgtc gtccggtaat
    38401 ttgctgcgga tggtggatac tttttcacgg acgtcctgga ctgcctgatc gatatctctt
    38461 tccagcacaa attccacgat ggtgcgcgaa acgctttccg tgctggtgga cgttatggtc
    38521 ttaacgccgt tgatggtgtt gacggcccct tcaattctgt cggccacatc aacatccatg
    38581 acatcgggac tggcaccttt gagcgtagtg gtaatggtga caatggggaa atccactttg
    38641 gggaataaat cgacgccgat gctggggtaa ctcacaatgc ccagcaccac cagagccatg
    38701 atgaccatgg tggcaaaaac aggacgtttg acggatgtat cagcgagcca catgaacctt
    38761 tactccgtcc gacaaattgt tctgaccgac caccacaacc tgttcttttt cattcaggcc
    38821 ttcgatgatt tcaatatctt ctccgtattt ccggcctgtt ttaaccgttc gggatcgtgc
    38881 cgtgttgcct tccacgacga aaagactgac ggtggcgtcg tcatacagca aggatgtcag
    38941 aggagcgaca acaacctcgc gcaacgctcc cgtgtaaatg gtggctctgg cgaacaaccc
    39001 cggtttcagt aaattctcgg cattgacaac aaccgcttcc gcctgcagcg ttctggttcg
    39061 ttcttccaga tgggggtaaa gcagatgtac cgtgcccttg aattttctgc cgggaaacga
    39121 atcaacggta aaatcaactt cctggctggt ctttaaatcg gccagttctt tctcgccgac
    39181 ggtgaaattc aatttcagcc ggtcgacctt gattaactga aataacggca tactggtacg
    39241 cacatagtct ccgacagaga ctcttttttc cttcaccatt gcacgcagag gcgagtatat
    39301 ctttgttcgg gccagccttt cccgggccgt atccagagtc gcttttgctt tggccaggtc
    39361 agcctccgcc agcgcaaccc gggtggagat gtcatcaaat tgctgctggg taatgagttc
    39421 ttccttaaag agagcttcct ttctctgaaa ttcggctttg acattggtca gattggcctg
    39481 ggcctgtttc agagccgcct gcgctctttt ttcatccagc gcataatcaa tatcgttgac
    39541 ttcagccagc agagtgcctt tgcccacggc agacccttct tccaccctga cgcttctgac
    39601 aatgccgtcc acttccgaac tgaccgtgac ttcttcgtcc gccgtcaagg ttcctgttgt
    39661 ctcaatataa ggccggatgt ttttcttgac cgacgtcgaa acacggacgt tggttaagac
    39721 ctctttctcc acgatctttt ctttcttgca ggacatcaat gaaaaagcga tgatcgctgt
    39781 aagtgaaatc aggaatataa acttgactgt gttgcgtcgg gattcttttt tttcaatgaa
    39841 gctgacaaga ttcttcaaat gcataacctt ttccctcctg gccctaagtc tttttttcta
    39901 cgattcatca atgctgaaaa acagcaataa cctgccgccc gatttttttg ccctcaaatc
    39961 gccgacgtcg gtcaactgcc ataatgcgcc gctggatcct ttgaccgccg gatcgccgtc
    40021 tctgacaaac tgcagtagcg ttccgcttga ttttttcacg gtcagagagg caagctggta
    40081 gttatataaa gcatcgacca cattcttatc tgctgtcaga agcagtgtgt tggcatccat
    40141 gacatcgaga ctggttgcca gaccgttttc gaattgtttc atcaccgcgt tgtaattatc
    40201 ccgggcaaaa atctgttgat cttccagaaa tttcagggaa cctttcaatg tgtccaggtc
    40261 gagacaggca gcctccactt caatatccac gctcttcttt aaatcgtcgt aagccaggcg
    40321 ggcctggcgc tctttagccc tggcttcccg gaattcagcc attctcaagc cgccttcaaa
    40381 aaacgggaag gtaagcgaaa tgccgccgta aatgttttcg tcattaaatg tcgagccggc
    40441 aggatcctgg tccgcccggt tataaatagc aaacaacccc acactgggcc agaaggcgcc
    40501 gcgggcgtat ttgacctgct gcccggccat tttcgtctgc atatcgtaac ttttcagatc
    40561 tgtgcgcaaa tccgatgccg atttgcgcag gccctggatg tcacacgctt ccgacaggga
    40621 tacggtttct tccttcagcc ggaaatccgg ttccaccccc gtcagacgcg tcagggcggc
    40681 gcgcgccagt ttcagggcat tggaggcctt gagatagtcc gatttggcgc cggataattc
    40741 tccctgggca cgcagaagcg cggttttggt cagttcgccg accttcatcc gcgtttcgac
    40801 cagatggtaa tatttggtga gtctttccag attggccacc gtaatatcca gagctttttg
    40861 ggctttgagt acgtcataaa aagaggaggc gaccgccaga agaaaatccg atttggccca
    40921 atccagatca tattcactct tggaaatgga ctggcccgct attctcaacg catccagttc
    40981 gcgggcgctc agcgagaaag tctgatcggc gcgcaccccc catgctgccg agtcttccgg
    41041 ctggatcatg atattcgccg tgttgtactt gtcttcggta aaccggttgt aggttccgta
    41101 ggccgtcagc cgggggatta aaagcgacag ggccttgtct ttgctcatct gcgcgacata
    41161 gacgttttcg cgggcgattt ccattttttc ggacgtatgc agcgccttct gataaatttc
    41221 acggagagtg tattcctgcg ctacggcgtt tgcggcagac agcagcgccg tcgccagcac
    41281 agtcatcata cagagcaaac tttttttcat catgaattcc tttcgcaaaa aatttatggc
    41341 cagatattgc ccatcgcccg ttgcagatgc gccaaacgga tgttgtaatc gcccagggca
    41401 ctggcgtatt ctgatttggc acgggttaaa agcgtttgcg catcaagtac ttccgtggac
    41461 gtggcgactc tttccttgta tcgttcttca gatatccgga aattttcctc agcttgtttg
    41521 atcaacgttt ccgagacgga gatctgattt tgcgcctcct gcagcaacaa ataagcgttt
    41581 ttgatttcca gagcgatctg gtcgatcaat tctttggagg attccgaagc ctggttttct
    41641 ctggctttgc tggcatcgac cctgaatttt gttctgcccc attcccagaa attccagttg
    41701 gcgaccgcca taatgtacca gctctccgca tctttaaaat cgctgccgtg aacggacgcc
    41761 tcgtcgccga atcgcgtata attaccgacc agacttaaag cgggataata ttcgctttgc
    41821 gacatgcgca ccagtttgcc ggcctgctga actttgagcg aggatatttt catttccggt
    41881 ctcagccggc gggccgtttc gaggcattct tcaaacgtct gcttcagcgg ttggtaagcc
    41941 agaatatcca tgacgtccac gggggcggaa atatcgcgtt tcagcagcgt attcaaacgc
    42001 gatttggcca gctccaccgc attttgcgtc cggaccaggg cctgtcttcc gttggccagt
    42061 tccacgtcgg cgaaaagcag gtcatttttg ggaatcagtc ctaccttgaa atagttttcg
    42121 gcgacatcgc gatgcgcttt gagcatttcc accgattgag cggttgcctc gttgattttc
    42181 tgcgcgcgca gaatattaaa gtaagccgct tcaacgtccc ggaccacgtc ctgaatccgc
    42241 gccgtttcct ccaggcgggc aacctcttcg ccgatgctgc tggcctgata attggccaga
    42301 attccgccgc cggcaaaaag cggttgtctg gcttcgatga tccagttgta attgtctttc
    42361 gttcccacgg gagtttccat gccgtttaag tcatacatcg gtcccggagg cagtccctgg
    42421 aatctggaaa aaggcgcttc gtttaaacgg gagtaactgt aggccgtgct gaacttgggt
    42481 agaaagccgg tcaaggcctc ccgtttctga gccgtggcgc tgttcaatcc ttctttcgcg
    42541 atattcagga tgttgctgtt ttttaaagcc aggtcaatac tggcttccag tgacagaacc
    42601 tcctgggcga agctcaaacg tgacgaaaaa acaatcatca cgactatggt caaaatccgg
    42661 atttttttac cgatcatgat ttttaacccc attaaaaaaa atgtccagaa tgaacccctt
    42721 tttggaatag ggggactcgt ttttcaatga taacatccat tccatggata ccgccctgat
    42781 caaataaacc agggattgag ccatgtcgcc ggccggcagg ttttttaacc gtttttgctg
    42841 aatcccttct ttcagcaagt tttcaataaa accgatatat ttaaaataat ccttgatgat
    42901 tttttcccgc atggatgtca tggcttccga cagagcggtg ctctcgccct tgatgaataa
    42961 acggcagaaa tcggcattct cttccaaaaa ccgcagctga gcatcaacca gcatctcgat
    43021 tttatcgagt acgttatccg cccgatcggt tgcctttttt gttttttcat acatcaaatt
    43081 gagtttttcg ctgatcatgg atgagtaaag attttccttg ccctcaaaaa attggtagag
    43141 agatcccgtg gaaaatccgg aggcgctggc gatttccgcc atggtgacat tatggtaccc
    43201 cttgacggaa aatatttttt ccgctttttc cagaatctca gcccggcgca tgttgaattc
    43261 gcgttctttt ctttccagtc tttccatcgc aaaaactcac gctgtgaata atcgttcaaa
    43321 ttttgaattg cggttcactt tttttatagc atattgacga tttgtcaagc caaatatttc
    43381 ggataatgca attaattcat cgggaaaata ttaaaaactt ctttctatta ttttctgaaa
    43441 tttaacgttt ctttaaacat catagctatt ccaggaaatt tttttcccct agcgaaaaaa
    43501 attattattc ttatatttta atagtttata aaataataac gccttttatg cattttggca
    43561 tacttatttc attaaaaatg accaagagtt ttcaagatca tcgaattaag gagaaaaaga
    43621 gctatgagaa aattaatgca aaccaccctc ttgaccgctt ttgtagttct ggtaaccacc
    43681 tctttcagtt atgctcagta cagcgttacc ggtgatgcca attttccgtt ctttcacctt
    43741 ggttgtttaa tcgttggcgg tttgatcatc atttccctga aaagaaagta caccaagttg
    43801 tatatgagcg aagccatcgg ttcttttgcg ctttacgctt tactggtgtc catttttacg
    43861 gcgccggtgg ttgatgccct taaaaacttg atcaactaac cccttttccc cttcaaagat
    43921 atacccttca ggaaccccgc gcagttcggc ggggttcgcc tgtttgtgcc acgcgtttta
    43981 aagaagttca tccagccggg cggagagctg tcgggacagt tttttcagga gacttgtctc
    44041 catatccgga gagtaacggt tctgaatggc atcgccgttg ttccagctgc cggcaacttc
    44101 tccgttgatt ttgaacggaa ccatcgccag cgattttatg aaatacttct gatttggcgg
    44161 aaataaccta tagtagggcc ttaagttttt attgaccaga atcggttgca ggccggaaaa
    44221 gaagatttca cgaaattgat cggctttgat gacattgagc ctgtccttta aaatacggga
    44281 tgatttgaag gcgtcgatga cccggcccgt ttttatggta tcggccacgc tgatccagat
    44341 gaatggcacc tgaaattccg cttcgatctg cgtaagcagc atttcaaaaa gttcggcagt
    44401 gcttccggct ccggacaatt ttttttcgac tttgtcaaaa cggtcggcaa tgtttgtgtt
    44461 gatgcgttca atattttctt ttttatccat tgcggcccct taccactgcg cgtaaagaat
    44521 caggtgatcg gatggttttt ccgccagtcg tgagtccaga tcgatggcgc tgctctttga
    44581 cctttcggcc agtgcttttg ttgccaggat gtgatcaacc cgccagccga gtccgcgttt
    44641 gaccgaatcc cttatacggt aatcgaaaaa agtatattga cccgcgattc ccggatggtg
    44701 tttgcgaaac acatccgtca atccccatga tttgatgtcc tcgagagctt tccagacgtc
    44761 cggattgaaa cagacatggc ccagcagtct tttcggatca tgaacgtcga tccgttcagg
    44821 cgcgatattg agatcgccgc accagatcag ttgattttgc ggccggaaat ttttatgcag
    44881 atatttttta aaacgggcaa gccattccag cttgtatgta aattgggaac tttctatctc
    44941 ctgaccctgg ggaacgtaag tattgatgat cgtcagatcg ttgaatgcgg caatgatcat
    45001 acggtcgggg tcggacggct cgctgtcaaa accgtaaatc accttctgcg gtttttgttt
    45061 tgagacaatc gcgacgcctt tgtactgctt gtcgcctttg aaaacaacat gataccccag
    45121 ggcttcaaac tctgccgcgg ggaaaaccgt gtcggcgact ttcgtttcct gcatgcagaa
    45181 aaagtccgga ctgtgagctt tcagccacgg aacaatgatg tgcatacggg agcgaatgga
    45241 attgacatta taagtcgcaa gcttcaatgg aatgtccctt tgttgatttt tttaattaat
    45301 atcatgaccg ggcattgcac tcaagttttt ttgcttaaag ctaccgtagc ttttttataa
    45361 atttgtcgta aaaaaagatg ttcagaaaca catccatttc atcggggcag catgcccgag
    45421 cataaaaatt ttttctgttc tttcctgtag aagatgcccc gcaacgttgc cagatgttct
    45481 ttctcccgat tcatcaaggt gaaattatgc tttatgacgg aatccgtcaa ctatttgcgg
    45541 ttggttttag catgaaaata attttctttt ccgttgacag cgcacccacc cttcttttat
    45601 aaacaatgga aaaaaacagg tttcccgaat gcggtttcgt gaaacatcat ctccccttat
    45661 catccttttg gtcatttctt gtcggatatg tttgatggcg gcgatgaagc atcgaaaatc
    45721 aaaagttgat ggttcatact caacagactg ttcaaaaata tttatggaga cattatgcaa
    45781 ctcatagaat ctgttctgca gcaaataagc gatatcctct ggggctggcc tctgattgtg
    45841 cttctggtcg gaacgcatat ctttctgacg ttccgtctca aattcattca acgctatatg
    45901 ttcaaagcca tcaaaatgtc catttcacga aagcgcgaag gggaggggga catcagccag
    45961 ttcggcgcgc tgaccacggc cctggcggcc acgatcggca ccggcaatat cgtcggtgta
    46021 gcgacggcga ttgccgccgg aggccccggc gccgtgctgt ggatgtggct gaccggcgtc
    46081 ttcggcattg ccacaaaata cggcgaggcg gttttatcgc tgaaataccg catcaccaac
    46141 aaagagggct taatggccgg cggccccatg tatgttctgg agcgcggtat gaacgcccgg
    46201 tggctgggat tgattttcgc tgcgctgacc gcagtggcgg cattcgggat tggcaacatg
    46261 gtacaggcca attcgattgc gtcgctggtg caggaaacct tcaatatttc accgtggata
    46321 accggactga cgatgactgt gctgacggcg gttgttatcc tcggcggcat taaatccatc
    46381 gcgagggtct gcgaaaaact ggtgcctatt atggcggtgt tttatgtctt cggctgcttg
    46441 attttattgg gcatgcatta tgaaacgata ccggaaacgt tcaaactgat ttttcaaagc
    46501 gctttttccg gacatgccgt catcggcgga tttctcggtg cgggcatgaa agaggcaatc
    46561 cgctacggcg tcgcgcgcgg cctgttttcc aatgaatccg gcatgggcag cgcgccgatt
    46621 gtagcggcgg cggcgcagac caaaaatgca attcgtcagg cgctggtttc gtcaacggca
    46681 actttctggg acacggtagt tgtatgcgcg ataaccggtc tggttctggt caattccggc
    46741 gaatggattc acggctacaa aggcgccgcg ctcaccaagg cggccttttc ggctttgccg
    46801 gttgtcgggc cgtttgtgct ttccatcggc ctgctgacgt ttgttttttc aaccattctg
    46861 ggctggtcct actacggtga aaaagccgcg gagtatcttc tgggaaccaa agccattcgg
    46921 ccttacaggt atctgtgggt aggcgctgtg atgctcggtt ccgtcctgag tcttcccatt
    46981 gtatggacct tcgcagatat cgccaacgga ttgatggcag taccgaatct catctcactg
    47041 cttgttctga ataaggtgat tatcagcgaa acaaaacatt ttctctggga gggtaagctg
    47101 gatgaacccc gctgatttga aaacgaaccg gtaacgtttt gtttgaaatc agtccaacga
    47161 acaataaacc ctttccgccg gattgcttcc tcaacggaag gatccggttt atggtcgcac
    47221 tgaacggatt cgtcaaactg ttacagtgac gattcctctg ttgaagtccc ggatgtgcca
    47281 tgggattgtc atttgaccac gcccggtgtg ccatatatca atgctgtcca tctcaagcgg
    47341 cattgtcttc caacacgatt cacaataata ccagggtgcg aaaaccttat ccacacaagg
    47401 aggcacccgt caccgaggcg gcaacgattc tgcttgattt gatccgaata tggcgccagc
    47461 gattaaacgg aacatggata gcgatcccgc tgatccgtca ggaaggagaa ccggtcggct
    47521 tctgactgac aagggattca atggctttgc gcttgatgcc gtccgttttc tcgataacat
    47581 cgacgaattc ggttttgatc gagacggaat cgtcaagcag acgacgcaaa tcggccagca
    47641 gttcttcgag atgggcgctg tcatactgat ttcctttgac gattttgatg acgattagat
    47701 cgctatgcac ctgtgtcacc ttgaactcct ccagcagatg aaggtacttc ttgatataca
    47761 tggtgaaggt gttcagaagt ttcagcgagg agacgaattt tccgctcgga agaataaagc
    47821 aatcgtcggc cctgccttcg aaggattgga gtagcgggaa ccccatgccg cagggacatt
    47881 cagcggtgct gaatcttccc atgtcgccga tacggtagcg aatgaacggc atggcagggc
    47941 tgcgggtagt cgtcagcacg atttctccca tgtctcccgg ggcaacctcc tgacctttcg
    48001 cgtcgatgaa ttccacccat acattgtccg taaagatatg gtagttgttg caccggcact
    48061 gggaggcgat catccaggtc tcttccgtcg agtattcatc gtagacggga cagccgaagg
    48121 tttcggagat gaaggtccgc tgggcctcag tcgaaagctc cgagttgatt ccgatgaact
    48181 tgggccggat atccttcagg tcatcagggg tggcgtttct cgcgatctcg tacaccagag
    48241 aggcataacc gatgatgagg tcggcccccg ccttcctgag ctgcgcgatc tgttcttcga
    48301 cggggatcgt cgatttgata tggacggccc ggaagaaagg gcccagacgg gtatagttca
    48361 ggtcggtata tttgatgtag gccattttgt gccatgggcg gtatccgatc attgtgtaca
    48421 tgcggacccc cagggcgacg tagaaggcat aggtcgcctg agtgaagatg aacggcaggg
    48481 acctgcctgt ggatccggtg gtggccgaat agcggcacgc ctctctgttg acgccgcgtg
    48541 ccacgatccc gtcgggaaaa ttctgccgga tttcttcctt ggtgacgaaa gggagccgtt
    48601 tgatgtcatc ggcggaccgg atagatgtaa tgtccactcc cgccgcatcg tatttctggc
    48661 gatagaaggg gacatggtca taagcatgcc ggatggagcg aatcaagcgt ctttcgcata
    48721 gtttcctctg gagtccatag gggagacggg cgctgaggtg gatttcaatt aaggatttga
    48781 aaaagtgata cagcaatatg gccgtcttca tcataacgga tacctcctag ttattgttat
    48841 tctctttttt attctttccg aaaggccgga tccagggact ggctccccgg ctgcctaacc
    48901 ggcggatcat attttctttc cagtcgtcga atatgtcata ggccgccggg acaacggcca
    48961 gggttaagaa cagagaagtg agaagccctc cgatcagcgt aatgcccatg ggcgctcgca
    49021 gatcggctcc ttctccgata ccgagggcga caggcagcat accgaacacc atggcaaacg
    49081 tggtcatcag gatggggcgc agtcggaccg gtcctgcctg aaggatggcc tcccgccgtg
    49141 acaggccccg ctcacgcagt gtgttcgtaa aatcgaccag caggatagca ttcttcttga
    49201 caagacccat gagaagcacc aggccgatga agctcacgat gctcagcgat ttaccgaaga
    49261 tcaggagagc cgcaaaagcg ccgataaacg acaggggcat ggacaggaga atggtcacgg
    49321 gatgaagaag actttcaaac tgcgaggcca gcaccatata ggccagcaaa attcccagca
    49381 gcagggcaaa cataaggtac ttgaaggact tgcccatttc ttcggcctct ccggagtaac
    49441 cggcaacgta tccagccggc agaatcctgg cggatatggc gtcaatttcc tgcatggcct
    49501 cgcccagcgg cttgtcctgg agattggcga atatccagat cgtgcgctgc cggtctttgc
    49561 gcgaaatgct gctcggaccg cccccttcct cgacggtgac gatgtcactt aatctgacca
    49621 gctcaccatt cacggcccgg acatagagtc tgccgatatc ggacgggctc atcctgtctt
    49681 tggcgtccag cctcatccgg acatcgtatc gtctcccctt tgctgtatct ttgaattttg
    49741 tgacctcgac ctcgccggac atgagaagat tcaccgcttc ggtgacggct gccacgctga
    49801 ctcccatatt cgttgccttt tcccggtcaa tgtagacact caactccggt ttccctgcct
    49861 cgatggaggt gtcgatatcc acgagcccct ttattttgga aagttcaccg acgatttgct
    49921 tggcatattt tctgagctcg tcgatggatc cgccgctgat ggaatattga acggggacat
    49981 tccggatccc gcctccgaga atggcaacct cttcgatggt ggttttcagc ccggggatcg
    50041 cggtaaattc cttccgcaat tccgccataa tctctgactg ggatttgtct ctatcagacc
    50101 gcggtttcag gctggtttgg atgaatgcct gattgatttg tgacagtcgc ccctgacccg
    50161 ctccctgacc atagaagacc gatgtcacct cggttctgtt ccggacaatc ttctcggctt
    50221 gactgaacat cgccccggcg tgttccacgg aataatcgat gggcgattcc atccggatca
    50281 taaactggct ctggtcttct cccggtttga attccttgcc gatgaaccgg gtaatccaga
    50341 ggctgaaaat aaagaggatg agggcgctca tcagcacagc ccggcgatgg tcaagactga
    50401 atgtcaggat ggggcggtag gcctcttcaa tttttttata tccgcgctcc agcgtctgcc
    50461 cgatccgcgc catcaccgtg cccgggcgtt cactgtccaa ggattctttt ttccgcgggt
    50521 ggagaagaag ggacgagagc atgggcgtga gggtgagcga cacgagcagg gaaacgagga
    50581 tggaaaagac gacggtcagc gaaaactgca tgaagaaacg tccgatgatg cctttcatga
    50641 aagcgacagg caggaatatg accacaatgg cgagcgtggt ggccatgacc gcgggaccga
    50701 tctcgtccgt ggcaaaggac gcggcctctc tcggagacat cccgtcttcg atgtggcggt
    50761 agatattctc gatgacgata atggcatcat caataaggat accgacagac agggtcaggg
    50821 ccagcatcgt cagattgtta aaggtgaaat cgaatatcct catgatcgca aaggttgaaa
    50881 tgatcgaaac aggcagggcg accgcgctga tcagggtcgt gcggaagttt ctcaggaaga
    50941 tgaacacagc gataatggcc aggatcgatc ccagaatcag gtgttcttga acctgatcca
    51001 tggattgttt gatgaacacg gactggtcga aggcgatccg gatatccatg cccggcggca
    51061 gacttttctt gatgcgctcg acttcctctt tgacacggtc cacaacctga acggtgttgg
    51121 taccggtctg cttctggata ccgataccga cggcggagat gccgttgaac cgtgcgtagg
    51181 aacgtctttc ctgggttccg tcctccactc tgccgatatc tctcaatctc acgggaacgc
    51241 cggcatagga ggcgatgatc agatcattaa aggcggagac atctttgaga gaccccttga
    51301 ctttgatgga gaattccttc gtcaagctct cgatacgccc gcccggcaat tcgatgtttt
    51361 cccttttcaa ggcagccgcg acatcgcctg gtgaaacccc gtaggccgcc agtttgttgc
    51421 tgtcaagcca gacccggatc tggcgtttct gcatgccggc cagggagagg gcgccgacac
    51481 cctggatttt ttgtaacttc tccttgaggc tttcatccac ataggttgag agttcgcgtt
    51541 gagacttgtc cccggtcagg gccagccaca ggacagggct agcatcgatg ttgaccttct
    51601 ggatgaccgg ctcgatgatg tcatccggaa gctgtgaacg gacggcggag atctgctccc
    51661 gaacatcctg gacggcaaga tcgatgtccc gctccaattc gaactccacg atgacactgg
    51721 acaccccgtc aatactcagc gagtggatcg tctttacccc gttaatcgaa ctgaccgcct
    51781 cttcgatttt gtccgtcaca tcgacatcca taatctcggg gcttgcgccg gccagaccgg
    51841 tcgtgatgtt gacgagggga aattccacct tgggaaagag gtcgacgccg attgacgggt
    51901 agctgaccag gcccagaacg acgagagcga gaatgaacat ggtggcgaga acaggccgct
    51961 tgattgaggt atcagaaatc cacatgatgt cgtatcatcc tttcttccgg tccgccttcg
    52021 aggcaaccag tccctgttga atatggtcca tcagtgtttc cagacggccg ccgccggcca
    52081 gaacgttcgg catggaatag atcatcgtca tatcatcatc actgttgcac atgccgataa
    52141 aaatggggga gtattcctgg cccgggggaa tgccccaggt atattttcct tcaaagcccc
    52201 ctccgctgaa aaccggaatc cgtcccatat tggtgaggac cccggagttg gggtagcggc
    52261 ccgtgcggtt cttggtggcg atattgcttt taatcagttt tttgagcaac cagaggggta
    52321 gaaagcgaat cggccggtcg ccccagtaaa tcatgccgtc atgccgcgct tccagctgac
    52381 gcttgatgtc ctgactcacg tcagccggtg ttgtggaggg attgatttcc acgtaaatca
    52441 gattggtcaa attccctgtc gaacgcaggc cctcaaccct ggcgcgcata tccaccggaa
    52501 tagccaggcg caccttaccg tcctgatact gccaggcttt tccggccagg agagtggcca
    52561 cctggcccag gacattgtgg tagcgtccgg ggtagcgccg tcggcgccag attacgccgg
    52621 gctgatctcc ttgaggcata ccggtgggag cgatgtattc cggaaccggc cctttgcgtc
    52681 cttgcttctg aaaggttgcc gcaatctcaa attcagtgat gcgcgaatcc gatcccaagg
    52741 gagcttcgcc gcgcagaccg cggaaaatat cttccaccca ggtcacggtg ccccggccgt
    52801 ccatggtggc atgcatcgtg cgaaagacaa cgcgcagggg gttgccgtgg atcagaacaa
    52861 cttcggatgt cgggccttca aaaggattca gggtttctga gagcttggcc ggagcgcctt
    52921 ccggaccaag accgtcccaa tgggttccat ccacctcgat caccggcggg gtttttccgg
    52981 tatcgacgag acgggcaaag gccgtgatac ccttgacaac gacgcgacta cccgggttgg
    53041 cctcggatgc gatttttacg gcttcccgcc atttgggcag gtcgaaatat ccggttcctt
    53101 cccaaaataa ctggttgaga aacggcggac agcattcatt gtacaccaca tatgcccgct
    53161 cggtggaact cagttgccgc gagtaagtca tatcgtatcc tccctttggt ttgtcccttc
    53221 cagtccatga acgattctct tcagggtgtt ttcaagccgg ccttcactgg ccagaacttt
    53281 gggcatggaa atcgtcagat ctgacccccc ttcatagccc gtcatgacca taaaaaacgg
    53341 atagtattca gcagccggcg gaataatcca gaatgcccgt gctgtgaacc ccccgccgca
    53401 aaaccggtta agattaatcc ggcccaggtt ggacaggatg cccgaaagac tgtaaagtcc
    53461 ttgccggtgc cgctcgcgaa tgatattttt tgccttgtat cgcatcatcc acaggggcat
    53521 gtattccaga cgctcgtctc ccttggtcag tctgcattca tcgcatcgac tgatttgcaa
    53581 agcgatgtcc cgggcgatgt cgtccgggct cgtttccttt ttgacctccg tatacagggc
    53641 aaaagataga ttggctgtac tgcgcagttc cggtctgcga tgtcgcaaat ccacggggat
    53701 accgaaacgg acaacccctt cgccatgtcg ccaggcttct tccgctgcca gacgggcgca
    53761 ttgtgccagc agatggggca tcttgccctt gagggaacag cgtctccagg tcacgccggt
    53821 atcattgcct tttgcccgtc cggtgggggc gatatgctcc gcggggaaag gcgtccggta
    53881 ctgattctga aaactcttac aaagctccac atccgtgatg tttgaggttg aaccgacgac
    53941 agcttcgccg cgcagggtcc ggaaaacatc ctcggcccag aacagcgtgc cccgtccgtc
    54001 acacacggcg tgatgggagc ggaatgcgac tctcggggtc ggaccctgga tcagaacaat
    54061 ctcgcaggtg gggtttcccc gataggggag agactcttcg agtaatggtt cattctccgg
    54121 gccccgtccg tcccagttcc tgccgtccac ttcgcggacg cgcatgttta ttcccgagtc
    54181 gacccatcgg ctccagctca ggtgacccct gaggaccaga cgggtaccgg gattggcctc
    54241 ggaagccgca tccgccgccc tgcgccattt ttccggatcg agcgtccctt caccgtcgaa
    54301 aataaattgg ttgaccgtgg gggggctgat ttgattgagc accacaaatg cccggtcatt
    54361 aaacgtcatt ttccgagtat accgtttcgt catcgcgccg gtccccctga cgatttcttg
    54421 tctgcatcgg aagttgccct cttgggtccc ggaccggagg ctgattctga aatggtcatg
    54481 gtggcgcgca caatggaact gacaagcgcg gcggtggctt tcaacgcctc aggagttcgc
    54541 gccgggcggg ggatctccat gtgaagcgca cgttccatgt tccgtgtgga gagatatccg
    54601 gtcgttttgt tgcagcgtgc tccgaagtag atcttatcca ggggctctat gcgcagctcc
    54661 agatcggggt aggccggata ggctgccttt gcctgcgcaa ggaaagcggg aaagcggtct
    54721 ttcaggcgcc gggcttcttc cgttgtgatt cctgtggtgg cgatttcaag ccggttgaga
    54781 aaagggatgc tggtgtgtcc gtgaatttcg acataaagcc gcaacggctt gccgtcacgg
    54841 atcttgtcaa tcaaacgagc gtattggtcg taaacatccc tcgccctttc ggtacgccgt
    54901 tctttttcgc acgtatgtcc tgcaccctcc gaggggcggt tgacgttgat gcgcggagat
    54961 ggcgacgcaa aacctccggc gatgacatag cctgcaccga gcgtccgggc cacatctttg
    55021 gccaggacat ccgtgcggat gtcataggtt ccatgcggtg ccgcaatgag aatgccctgc
    55081 gtcgtcgata gtgtttcaaa atgaccgaga gcatgtaaaa aatgctcttg tgcgacagcg
    55141 gtttgaatac tgaaggcggt gaccagcatc acaaccgcca gatgcaatgc ccgggacatc
    55201 gtttggtcgc ggccattctt tcttttacgg agggcgctga tgagatacag gtttcctcta
    55261 ggttcctttt gaagacgggg cactgattga tcaaggggat atggtgcagg cctgccgatc
    55321 atcctcaatt cctttgttca cttgtgctgt cctctcccgc aggaagaccc ttgatatgtt
    55381 tcgcatagag caggccgatc aggtaacgca acaccaggat ggaggtggtg tagatcagtc
    55441 cgaaagccat gatattcagg ggcgtcccgt aatccttgaa gagaaggagc atcgtcgcct
    55501 gtgccgtgcc gaatccgccc ggagcgatgg gcagactgat gaccaggaga atgatcggga
    55561 cataagcgat gagcgcaacc agcgggattt gcatgttgaa tgccctgacc gcaatataga
    55621 agaaggcgac aaagcaggca tagtagagaa tgcgccagaa gatgaggaca ccgtaggttc
    55681 gccagggagc ctcgtgaaag accttcagaa ggttcatgct tttaattttg agggccagac
    55741 cgcgggacgg cagttttttc aacacgataa taaaaagaat atccagcagg gcaaagccga
    55801 tgaagaagat cagcaaatta cgcagcaatc ccgaggccgg agaaagaatc accaagccga
    55861 tgatggccat gtaaaccaga gccttctggg tgatgccgtt gaaaaaggtc atcagtcccg
    55921 ttgatcgcat gaccgggatg ccgagatggt ttttcagata gtagatcaga actcccatgc
    55981 ccacactgta gtcgatcgtc atgatgagat aggtgacgcc gcgcagagtg aaggcatttt
    56041 tccaggaaag ggggtaatga aaatgcttca gcgtctcagc cagattctgt gtgtccagaa
    56101 gaaaggtgat cagaatgagg gcaatcaaag agggaatata cagggacaca ttcgcggtca
    56161 gcatggcatt gaaacacatg cggatatcaa tgtgccggaa caaataaagg aggattgcgc
    56221 cggagactgc cagagggagc agctgtttcc atagtttggt attcatgagg aaagggcgtg
    56281 ccctttcctc atgtggaaaa tccatatccc ccatcaccgc ttgagagttt gatatcttgg
    56341 ctgtcttgtc catctaattc cgtcatcgaa tacgtcttca ggggactctt tcaaaaatat
    56401 gaccacggga gggatcttcg cagtgcccct cgtccatcat catattagta ttgccaggga
    56461 aaggcatccg cagagccggt ctcgaagttt tcataagcat aatccgcgca gtaatccgca
    56521 aaatccttta gattgaaagc ggtaatctgt gccgggttgc tttggagaga agacaggaac
    56581 gattcggcat tatcaatgat cgtatcctcg atctggaatg tccactgctg aaccttggcc
    56641 agttcgacct tattgatgta gttatcgtac atgccccaga tgacatctcc ttcattcgat
    56701 gaataaatga gactgccgta ggtttccctc gcgctgatgg cctgattgag cagacaggaa
    56761 tcatcgtaat ctctccaggc accgttggca tacgtatcca cccgggccag ttccgatgtt
    56821 cccctggcgg cggcaaaaaa cgggacgaaa acacctacag agggctctcc gaggttgacc
    56881 cacatggtgg ccaggcgcgg gttgtctccg gatgcaacgc ccctgacgac gatcgagcta
    56941 cgggtggcat tgcggctgag gcaataggtt gtgctgtaat tggtctcgct ggaaagatcc
    57001 gtctggacct gcttgccgac aacgtccttg gccacctctc gcataacggt gcggtaattg
    57061 atccgcttct ctgttgccaa ctgggtcagg agggcttcgg ctctatactt gcgatcctgt
    57121 cccacgttcc agggaatgta gtaattgtaa ttggtcataa tgtggaatcc gatgaagttg
    57181 ctctgagccc gtttgagcgt ccatccatac tgattggtga tcttgcccgt gttggcatcg
    57241 ttctttctga actgcatcag gttgaggccg gtgtttagat ggccggtgaa gcattcatac
    57301 agtgcggctc cgcctttggc gtcgatgaca acgaagttgg cgctgataac ggtgccccag
    57361 tgatagtaag gccatctttt gagcagtgat tcaaactccg cgagcgtggt acatgtcgcc
    57421 agggcttcct ccataagaac ggtattgatg ttcaccggtt ccgccataag attataatcc
    57481 tggtacacgg acgtgccggc aatggcaaaa cctgattcat tgacgccgcc ggaaggagtg
    57541 atcgccgagc cgttgttcag cagtttcatc ccgttttcaa accggtagac cattaaatat
    57601 ccgccgggac cggacttttt ggcggggaag taggcaacct gctggcggtc gcttgacgag
    57661 ttgtcaaagt ttttccagat cagcggtttg ccgtctgtgg tgaccttgcc ggagacaacg
    57721 gccacatcac aggcccatgt ccgcgtcgcg aagataaggg ccaataggca catcaagacg
    57781 caggaaattt ttaaataacc cttttgtaac ttaatcattg gagcctcctc atttactggt
    57841 tgtttcaaac gttaattctc ttacgtttag ttttgaatat cgtatattct tctgcaaatc
    57901 attttatccg tgatccgcag ttttgactga aaaaggttct ccggacagac ccgggacccg
    57961 ccggtcggta aaagacggga taaggggtct tctgctgctg aacagtccga cacccctgct
    58021 ggaaaagtat tccttgccga agcatttgat catcagaacg aaggtgacca gtcgtacgat
    58081 tttcatgggg atcatcagca taaaggcaaa tgcgtcgccc ctgtccatat gttcgaaatc
    58141 cgccggcatg tttgcccgga catggggcga gaattgatac ttcggatcat tgggatcaaa
    58201 accgtttgag ccgggaaaca tgttcttcag gcagaaagga tagctctgat ccacaagtcc
    58261 gatcccgtat ttacggcaat actcttcctt cagagcgatt aatgcgggcg acggggagaa
    58321 agaggagtca aacagtaccc gcagccatga tttattgaaa taccggttga ccactgcggg
    58381 attagccgag atggacgtaa aaatgaaata atctttgttc ttgattgaag cggctttcgt
    58441 gaggtgccat tccaccgtca taatgaccag acgttgagca aggtctcttc cccggaatgc
    58501 tttgagcacc atcgtttctt cattattgta cacgcaatgg ggaccgctgt tgagaatgga
    58561 cacggcatca aaggcgacga tccggccgtt tttttccaca tagctaagaa catcgcattt
    58621 ccgcagatag gttgagaatt cctcctgcgc cttccagcaa tcattttcaa ccgcttcaca
    58681 tccccgaatg atatctccga gacgattcca ttcggctgta ttcaaatcga tcaggtagta
    58741 attgaatccc ttgtggctct gaatgaaaaa atcctgagcg tttaccggta gggggtccgt
    58801 tgaatcgctt tgatgccccc tctgctcact tgctgacgaa acagacatgc ttttcatgtg
    58861 tattcctcct cttaatgatg cagttattct aaagagtttg aatgccaata gatgacacct
    58921 atcggctgac acttttcgtc gtcaaatccg atccgttatc cggacagatt ttcatggacg
    58981 aatctggttt agatttttac taatctctgg ttgaatgccg ggtctcttca gataggaggc
    59041 aacgtaccgc ttgatcatga acgcgaaaca acgtactgct tggtattatt gcataacaac
    59101 ttagtaaata tattctataa agagcacgca aacgacctct ttatgaataa tctttcacaa
    59161 aaaacgcgat atcctcaaat taatcatttg aattgaatgt tcggtcaata gtatgattga
    59221 tgggtaaagt caataaaaat tttgtcatgg aataaaaaaa tatcataatc aagagagcat
    59281 ggagtggaaa taaccttctg aaattctaga ttcgtcaata tcttcgcaca tttaagcgaa
    59341 ggatgaaatg ccctcaataa catcctgaca ggtctgtcca atttccacgg ggaacaaatt
    59401 tcataaccgg gcatcgaatt tgagtccagg ggtattcacg ataagaaata cccggaaggg
    59461 aaacaggcat tttcattgag ctatcccgac gctaaaagct tcagaaaaag aagcgctttc
    59521 gggtttttta caataggttt acattaagta catttcaaac gcccgctgtt tttcagaaac
    59581 gaagaaaccc gcagggatcg tgaagtttct ggaaggcaat gcgtccggtc ttgaagacca
    59641 atttgcctta ttgcggtttt gctgcggggg atctgtccat acggattttg catatcgatg
    59701 ttcgaaaaca gcctttgatc attccagacg acatatccgt caaagccatg cgccggaaaa
    59761 tcgtataatt ttattcattt ttaatatttt aaagacgtgc tataatgcaa cttaatcaga
    59821 tatctcaaac attatgaata aaagaaaaaa gctgggtgaa atcttaatcg aggacagcat
    59881 tgtttccgaa ggccagttga tgagtgcgct tgcccttcag aagaaaagcg gcaagatgat
    59941 cggtcagacg cttattgagc tcggttacat cacggaagca cagcttgtcg aggctttccg
    60001 aaagaaattt tctatttcct atatcgattg tgatggcgtg gttgtgccgg aaaaatttct
    60061 taaactggtc agtatgacca tggccgttaa gaaaaagatc ctgccgaccg atgtgtcggg
    60121 gaatacgctt ttcctggcca tggtcaatcc tcttgattat tccaccatcg atgaagtgaa
    60181 atttctcacc ggaaaaaata ttacccccct gctgaccaca gaatcgtcgc tcatgaaagc
    60241 cattgagaaa aattacggct attgctccat cgaaaaaata tggaagccgt tgaacgatat
    60301 ccatctggac ggcgaaatgc agttcaatct ggataaaagt atcgagttcc ataaaatgga
    60361 agaggaagat gaaagcgcgc tcagagccga tgacctctat aaaataggag aagaaccgcc
    60421 cattgtcaaa ttggtgaccc tgattctggt caatgccgtc aaatcaaggg caacggacat
    60481 ccacatagag ccccatgaaa aatatgtcag tgtccgctac agaatagacg gggacctgtc
    60541 caccgttctc aaaatatcca aaacgtatca ggaggcgatg acctcccgga tcaagatcat
    60601 atccaccatg aacattacca tccgccggat gccgcaggac ggcaggagta ccgtcaaata
    60661 tgaaggacgc atgatcgatc tgcgtgtttc caccatcccg tcggctcacg gagaaaccat
    60721 tgtcatacgg attctggatt cggtcaaggg actcatcccg ctgagcaagc tggggatgcc
    60781 ggataacatc atcagagatc ttctggagat gctcaacaaa cctcagggga tgctgtttgt
    60841 aaccggtccc acaggcagcg gaaaaaccac cacgctttat tccctgctga cacagctttc
    60901 gctggaatcg gagagcgtcg tgacgatcga agacccgatc gaatacgagc tggagaacat
    60961 caagcaggtg ccggtcaatg aaaaagtcgg gtttacgttc gccgcggctc tgcgttcgat
    61021 tctcaggcag gatcccgacg tcatcatggt cggcgaagtt cgggattccg agaccgctca
    61081 aatttccacg aaggcggcgc tgaccggcca tctggtactc agctcggtcc ataccaatga
    61141 taccattgca accatctccc gtttaaggga tctgggcata gaaccttatc tgatcagttc
    61201 cgctctgaat gccatccttg cacaaaggct tgtgaaaaaa atatgtgaaa actgccgggt
    61261 ggaattcgaa ataccgcagt atctttttga gaactaccct tatctgaaaa agctcaaaat
    61321 cggaatatcc tataagggga aagggtgcga agcatgcggc tttacgggct ataagggacg
    61381 gatagggatt ttcgaattcc tgcgggtcga cggcaatctg agacggacca tccccctgta
    61441 tcattccgat caggaccttt gcatcgttgc ccgcgaaaac ggcatgaaaa ccctcattga
    61501 agacgcttgt gaaaaggttg aacacggttt gaccacgatc aatgaagttc tgacaaaaat
    61561 acactactac gaatagaatt tttttcggga aaccttctgt ttcgtctcaa cgcttaggct
    61621 gtcgcagctc ttcaaaataa gcatccgtca cattatccag cagctgcagg gtggtcggcc
    61681 ggacaacaat catccgggtg tcgggatgat tgaaaaaatc cctcaggtcg ggatgcttcc
    61741 ggagaagcgt ttcccggatt tcctcaatac ttttatcgtt atccacagcc tcaacgatgc
    61801 cggtgaccgt caatgcccgg ggagttcctt cggcatcgac aacacgcgta tcgatcagca
    61861 gactgacatt gggattggcg atgagattgt gatatttttt tgtatctttg aaggtggcca
    61921 tgtagatttc acggccatcc cgccctgatg catacgacat cagagaacaa tgcggggcat
    61981 cccggccgac cgtggccatc acgcacatcc tgttttcctg aatgattttt tgaatgtcac
    62041 tgcgcatatc ctctctcctt tcttccggca ctcggagtaa ttttagcata aaggcgacgg
    62101 aaaagggcag aacttttaac ggccaacgcc tgcaaaacat ttttgacaga cggatgaacc
    62161 ctgtgttaaa aacgatacgt gaaaaacaaa aaacctttct taacagaagt caggtcgcag
    62221 gacgttttat ccgtttccat tcccgatacc cggtcgtttg ccggagccca aagaaaatcg
    62281 ttccgtgtct ggtggattgc gtttcgctat cattttgttc ccgccagttt tcttccggct
    62341 atcctggggt ctgtcggcgc ctgggtgcag accggtctca tgaaccgatg gtttttcttt
    62401 ctcacggtgc tgggcgttac gctcaatcac atcgccctga acatgaccga cgactatttt
    62461 gattatcacg ccgccgtcga tcaaggcggt gaacgccggg aaaatcctta taccggcggc
    62521 agcgggaccc tgaccggcgg cctgatcaaa cacgggcaca tgcgcctgat gttcacgtta
    62581 ttttacctga tcaccatcgg gatcggcctc tatctgtgcg cggcgcagac ctggtggatc
    62641 atcgttttcg gcctgttcgg cgtggggtgt tcgtattttt acacggcacc gcccatccgg
    62701 tacggatatc gcggcctggg agaattctcc cagttgatca atttcagctt taccatcggc
    62761 atgggcgcct atgtcgtgca gactgtttct ttttcgtggg agattttctg gatcctgttg
    62821 cccctggggt tcatgatgtt tgctatgatc gtcatcaatg aaatcccgga cagattcgac
    62881 gatgccgcgg ccggtaaaaa cacgctggtg gtgcgattcg gcctaaaggc ggcagcccgg
    62941 ttttacggcg tggcaatgat gctggcctac gcggtcattc tgcttgcacc tcctctgaac
    63001 atggcaggct actggattta tctgggcttg ctgaccctgc cgtggtccgt tcgcgcattt
    63061 ttaatcatga aagcatacgg caacgaaccg gacaaactgg cgcctgcgaa tttactgacc
    63121 attcgcgttc ataatttcac cggaatcttg ttgatcattg cctgcctgat tcaggggctc
    63181 caggatcgtc aagtgtttca accggttgtt tttgccgcgg tcatcctgtc cgctttttat
    63241 ctgccggcgg cgatcagtat tttctttaaa aaaatgccgg atggccgccg cactgaagca
    63301 tgataatatc cagtcgcatt cggtcttgaa agccgggcgg gaaaacatta caatcattat
    63361 aattaatacg gggagggtat gctatgaaga tggacaagga gaccttgtcg aaacatgacg
    63421 gcagggaagg ccacaaagcg tatgttgccg tcgaaggcag ggtctatgat gttacgggaa
    63481 accgcctttg gaaaaacggc gtgcatatga accgtcatca ggccgggacc gatctgacgg
    63541 aagccattgc agcctcgccg catggaaagg acgttttgtc caaacttcag gacatggggc
    63601 agatgagccc tgaaaaagtg gccaagccgc cgttcattcc cctgtggatg catcattggc
    63661 ttgaggccta tccatttttt aaacggcatc cgcatccgat ggttgttcat ttccccatgg
    63721 ccatttttat catcgctcct ctttttcttg cctggtatta cctgatcaat ccggtacccg
    63781 gactgccggc tgctgttttt tatctctaca ttctgggtac gctgtcgctg ccggcagcca
    63841 ttgcgacagg cctgctggcc tggctggtta attattcggg acagccgaac cctctgatta
    63901 tacgcaaaac gatattttcc ctgttgctgc tgctttgcga cggaatcgtg atggcggcgc
    63961 tcatcatcag ccccgcaatc ctgcaggatc cgtccggaag cgagtggatg ataccggttc
    64021 tgatctttct ggggctcccg ctggcatcca ttatcggcga gcacggcggc aggctggttt
    64081 tcccggtgtt gaagaacaaa tagcggcgga cgtcggaaga tgccattgcg gggcggttta
    64141 taacgtatcg tttcgtctct ttgatggtga ccgatcaaac catgaacgaa tgattttttc
    64201 accgcaacct gccggcggtt tgccgggaac agcccttttc tttttattgt gttattgact
    64261 gataaggttt aatatagcgg tgtccctgtt acaactcctt cggatatttg cgcccctggt
    64321 tttctggcaa ggtacgttag tgaagtccat aacaaaccca catgcacaag gataataagg
    64381 aggcctgcca tgccatcaaa aaaactcaaa gagtttctga acagccaatc ggtgaaatac
    64441 gtcatcattg cccattccac ggcatacacg gcacaggaaa ttgcgcagtc tgcgcacatt
    64501 ccgggaacgg agctggccaa gaccgttatc gtttacatag acggcaagat ggccatggcg
    64561 gttctgccgg ccagctattc catcgatttt gatgatctcc ggaaggaaac gggcgcaaag
    64621 aaagtggaac ttgcgtctga agcgcaattt aaggatatgt ttccggattg tgaaatcggt
    64681 ttcgtaccgc ccttcggtaa tctttacgga atggacgttt atgtggatga gcatcttacc
    64741 cgcaataagg aaattgcctt caatgccggc ggtcatctgg aactgtacag aatgtcttat
    64801 cgggacttcg aagagctcgt aaaaccgaaa gtgctgaaat tcacctgaac ggacatgatg
    64861 ccattcgccg tctcttccaa acctccgttt attaaataat gagagaaagt gttatggagt
    64921 tgaacatcat ccgcaaggcc atggcgcata aaacaataag tcttcacgtc ctcctgacgg
    64981 tttttttagt gacgaattac agcgcaatca ccggcgcggc agagacagga aaaaagagtc
    65041 tcctgccgga tcggtctgtc atcacgcttc ccgctcccgt gtatcaaggc aggatgtcgg
    65101 tggaagaagc tttgagaaaa cgtgaatcgg taagacaatt ttcacaaagc ccattaacgg
    65161 agcaggaatt ggcccaactt ctctgggcgg cgcagggaaa gacgcgtgac tgggcagcta
    65221 ggaccgcacc gtcggcgggc gccctgtttc ccctggaggt ttatgttgtc atccgggaag
    65281 gcgtcttccg ttattcatcc ggagaccatc ggattttgcg tgtcgtcaag acagacgttc
    65341 gccggcagtt ggctgcggcg gcgctcggtc aggacatcat ccggcaggcg cctgcggttt
    65401 ttgttattgc cggtgtctat aaacgaaccg cagaaaaata ctcaaaccgc gccaaaagat
    65461 atgtggagat cgaagcgggt catgccggtc agaatcttct gctgcaggcc gtgtcgctgg
    65521 gattgggcgc tgttccggtt ggcgcttttc aggacgcaca cgtcaaacag gcgctacatc
    65581 tgcccgtcaa tcatgagccg ttgtatctga tccccgtggg cagaaaacgt tttgtccccc
    65641 cgtagaaaaa aaagtttccc ggaattttat atcgaagatt ttcttgacag aaaaacgcgg
    65701 ctgccttaaa cttttatgac ataaaaaggt ttcgctgaaa ttttcccaaa atcaaatttg
    65761 tcgttacaat gcttttgaac tgtaaatagt caggaaggtg tattttatgg gcgatttcga
    65821 caagagaaaa tacatccggt atcccgcgga gccgaacgaa ctggttacgg tctactatat
    65881 ggacgagaaa accacaaaga ccggacaacg cgtggggcta ggcgagaatg aaagttttaa
    65941 ggggtgctgt gtcgtttttg tcggcgacgt ggatttcaaa cagggacagg aagtgttttg
    66001 cgaatgtgga acgcttccca aaacaaaagc ccaaatcgtc tgggttaaaa agctggaaaa
    66061 ccagattacc aaagtcggaa tctctttccg ggaataactc ccgactctaa aggtcaacga
    66121 tttcatcttc atgacggaac ggtttttatg atccgcgctg aaaaacggac acagcgcgaa
    66181 aatgaaagcg acgtttcaaa aacaaagcgt gttgaaaccc atggattgag ccgtctccct
    66241 tgccgacatg aaaaatgaca taaatcggca gccgcgacaa gcgttgctgc tggagaaata
    66301 aaataacctg aacgttatgg ctgaattctt tgagaccttt attccggctc ctgtcttcga
    66361 cgcaaagaaa aagggaggac agtcggttag cggttttttt tcggcagaaa cgcatggaac
    66421 ccgtttttga tgatttcact gaagtgcaga gttaaatcat cgagcgacat ttttatccgg
    66481 ttgttaaacc agtcctgata caggccgaca aatccggaag catacaaatt gacgcaaacc
    66541 ataaactgtt ctttgttttt aatggcagac attttttttt catcgcttac tattttatct
    66601 aaaaaaatcc ccttcaattt attcaaaaaa attatcgatc gctgggagtt gatcagaatt
    66661 ctgtaaaatt ccaaatcttt ttccaggaaa cgggcaatat tcatcaaaaa tggcagaggg
    66721 ttttcgatca gtctgctgtt tttaaattca tcaagaaact ccagtaatgc cgagatgatt
    66781 tcattgccaa tctgttctcc gacggcatta acattgctgt aatgggcata aaaagtgccg
    66841 cgattaatat ccgctgttgt gacaatatcc atgacggtga ttttatcgat atccttttcc
    66901 ttgatcaatg agacatacgc ctgcttgatc aatttcctgg atcgaatagc gctgcggtac
    66961 tctgcttttt cgtggattgt ctttccgctc atttttgatc tcctgtaaaa aatctgccta
    67021 aagtccgaca aatttaatcc ttatgttcaa ttttatacaa tatctccaaa tattgagtat
    67081 tgactgaaac acaagtgcct tatattgtac ttatgttcaa aaatcaacat gtgtttgaca
    67141 tttgaacaaa ataattggcc ggaacgcggc cctcgaaaag caggcgggca acatgaaagg
    67201 ggagaaacaa tcctttcaac agaacctgaa aagtaaacta tgacacgatt ggcaatgaat
    67261 ggatcatgct tgcttaaaat aaaaaatccg gaggcaatcg aacgacacaa aaaagcccct
    67321 ccagcggtta aaaccgcttc ttcttattcc tgtcctaagt gacccctggg ttatgacggc
    67381 aggttccttt aacaccatcc ctccagcaat ggagggatgg tgcctttttt taacgtcaga
    67441 ccgttttaaa acatcgcatt tttagaaaac aaaaaaagta ttttcgaaaa aaagagtgtc
    67501 gggaaaaaac gatgaacggc tttaactccg gtttcaggaa actgacagcc gatagtgcat
    67561 ttctatgatg gttcgaccgt attggtgaat gccggccctg ctcaattgtt tttgaagggg
    67621 agacggtttc ccgtcgggaa ggaacgattt gccccggcat cgtgaaaaat cccgaacgga
    67681 cattttacat ttacactccg ggtccaggca attatgccgg gccgttattc agatcgcaaa
    67741 acgatgtctt caagggcgca ttccttacgg gcgcatggat aaatgattcg cctgaagaaa
    67801 atccgttcct gccgacggct caaatattat tttgcttaat cctttttcgc ttnnnnnnnn
    67861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    67921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaatccg ttcctgccga cggctcaaat
    67981 attattttgc ttaatccttt ttcgcttttt tcagatcgat atgacagtag ccgcggggat
    68041 ttttcttcag gtagtcctga tggtaatctt ccgccggata gaaatctttt gcaggctgaa
    68101 tttccgtgac aatttttctg gtatatctgc cggatcgggc aagtttttct gcagaggctc
    68161 ttgccgcttt ttcctgttgc ggattggtat aaaatatcgc cgaccgatat tgggtgccca
    68221 catcgttgcc ctgcctgtta aggctggtgg ggtcgtgcaa ttcccagaag tgttgcagca
    68281 gccgttcata agttacgatt gtcggatcat aggtaacgcg gacagcttcg gcatgtccgg
    68341 ttttgccgct gcagacctgt tcataagagg gggctttaac ggttccgccg gtgtaacccg
    68401 attcgctgtt gacgacgcct tttacccggc tgaaatactc ctccacgccc cagaaacagc
    68461 cggcggcaaa aatggccgtt tcggtcgatt tgccggatgg agcagcaaat aacgacaagt
    68521 actcaccgta gccttccgtc tccattttct ctcggggaat aaattttagt gatgcggagt
    68581 tgatacaata tcgcctgccg gtgggggcgg gcccgtcgtc aaaaacatgc ccgagatgaa
    68641 tgtccgcttg cgtacttctg acttccgtgc gtaccatgga gtggctgaaa tcggtctttc
    68701 tccagacggc gttgtccttg atgggttttg taaaactggg ccagccggtt ttggaatcga
    68761 atttatcctt cgaactgaac aggggaacgc cggaaacaat atcgacatag atgccttcct
    68821 gatgattgtt ccagtattcg ttttgaaaag gcggttctgt ggcgttgccg aaggcgacat
    68881 cgcgctggag cggcgtcatt cttttttcgg catcctgtcc cgaaattttt ttatccatga
    68941 ttctccctcc ctgcacccgt tcggacgtaa cttgcgccat taaatatacc catatgaatc
    69001 caaaaagcat gacaagaata aagacgggcg tgtatttgtt tttcatcatt tcacctttaa
    69061 aagaaattta atcattgtcc gacaagagag caagatcatt ttaatcaacg gataaattta
    69121 agcaattttt ttgtcaggtg ttgagacgat ggccgctctt tggggtcatc ttgctgtcca
    69181 ggaaaaattt ccggagtttg tctccgtaac ggcttttgcg ataaattttt aaccagcggt
    69241 tgacgatggc gcccgcgtat gtcctgacaa acagatttct ccatattctt ttgatatctt
    69301 tccaggcgat gatcagcgga acggcaaaaa tcgtccgcgg actccaggcg ccgtcgggcc
    69361 gtttaccggc acgttgtctt tccgccaaat tacggataaa ccggcggatg taaaattcga
    69421 cgaattgaat gggaaatccg attgtggcaa tataggtcca gttcagccag ttgccgtaat
    69481 tcaaattccg tttgataaat cctccgagat attttttttt catcagcagg ccgggcatgc
    69541 cctgcagttc aaatgcgtcc atactgtcgg gccggggatc gtagcagagg aaaagcccgt
    69601 cgtatttatc ccagcccact attttcaggg gcagaagacg gccttcccgt tccagtcttg
    69661 ttctgagatc cgaacccgga agcggaaccg cattcaatac ctgaatggta tcgataccgg
    69721 attttttaaa aaaatctttg tagattttcg cccgttgttt gacggtgaaa gaagatggcc
    69781 cctgtgcatc gtcgttgagc ggatagccga aaatgaacat gccgtgcaga taaaaaacat
    69841 tggagagttt tcgggaacgg gcaatcaact tctccacggt tacgccttta cgcatggccc
    69901 ggagttcttc attgatcggg ctttcgtacc cgatcgccag catgctgaca ccggcaaaat
    69961 gcatggcctc gatgagggcg tcattttccg ccacttccga acgcacctga accaccaaat
    70021 cgattttttt caaaaaattt cgtttataat ggccgatggc tttgcacaga gcaatggcct
    70081 cgtccggatg ctgtgcaaaa ttatcatcgg tgaaaaagaa tttagtgtat cccatatcgg
    70141 catatcggat cagttgcctc agcgccgtat cgatggacgc agccttgtat tttccgtatt
    70201 gtttgttcac gacgcagaat tcgcaattcc agttgcagcc gcggccccgg ttgatgggga
    70261 tatagctcca tcttttttgt agatatttga tcaatgtcag gtcgcaatcg gccaggccgt
    70321 ccaaatcgga aatgggcggg cgcgtgccgg taaaaacgta gccgccgccg tcatccagaa
    70381 aactgattcc tttgatctga gacaatctgg aacggttcag atcgacaccg ctttcggagc
    70441 tc
//
LOCUS       DAOSME010000004        65369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_555_length_65369_cov_20.552378, whole genome shotgun sequence.
ACCESSION   DAOSME010000004 DAOSME010000000
VERSION     DAOSME010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 65369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65369
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_555_length_65369_cov_20.552378"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLG98_01050"
     CDS             <1..1215
                     /locus_tag="PLG98_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE31866.1"
                     /translation="NRLARDQEVRQAMADFFPRIETTLSDGYIDQHHPYLLDDEFHPR
                     SATVRLNQNIFRGGATFAETKRQKSRVISAAYQLQGVSENTALLACRVYLNYLRAIEQ
                     DNLAKENLLIHERFYDQIKLRSQAGVDRRVDLDQITARLALAKSNLIVTQANIENAKT
                     DYQAVMGYLPVNLVKPEPAYAEIPATLQDAERIALASHPTLKSAKADIEARNYQHKTA
                     RALAYPTVDVGAGYTWGEDLNRPAASYDYEDYLQLNATLSFNIFNGFINQARIKETSY
                     LINEAEEIAKKTELQTIQSVRLSYESYLADQRRVRELEQYVDSTDKAAEAFISQWSIG
                     RRSLFDVLDSSAEKISAKSDLINAQYDKMYSAYRILSGLGNLVHTLGLQWPEESRVDT
                     GAGIVDMSFLEK"
     gene            1417..2289
                     /locus_tag="PLG98_01055"
     CDS             1417..2289
                     /locus_tag="PLG98_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOE31867.1"
                     /translation="MKYWKITGVISAALCLLITYQAWADREWVYLESNSSRESFYDKN
                     SIVKVDENVFRLWMKQVLSENGKIKTISKFRNTNKPDNSLDLISYILRQMDVDCRNNQ
                     YKDSALIFYDEKSNVLYSSPKGAIGKWDDIVEGSFAYQLKNVICKGPNLAEKPSAILS
                     ADHEEKPAEADTPPQSLPAGTSDEDIRQVVSKWLAAWESGDIATYRSCYDEDFKSKNM
                     TLEKWIAYKTGVRQKSTGIKIGIDDLKIIADGDHAEAVFIQTYSSSLLNDKGKKTLKL
                     KKVGNEWKIYQEIM"
     gene            2610..3482
                     /locus_tag="PLG98_01060"
     CDS             2610..3482
                     /locus_tag="PLG98_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE31868.1"
                     /translation="MDYEGFIIRPPSEAYSLLLQVTTGCSHNKCTFCGTYRQKKFKIK
                     PIERIKKDLDEARQYEDVDRVFLCDGDALIIPQPKLKEILELINENLSDIERIGTYAN
                     AKSILRKSVEELKELKSLGLKIVYLGVETGNAELLKKIHKGVTCEQMIQAGRRIKEAG
                     IILSVTVLLGLGGREKSIDHALDTAKILTDMDPDYVGALTLMLIPETEMYEDYLAGRF
                     VLPDQFGFIRELYLMIEKSSFTDCYFTSNHASNYLPVKAHLPREKEKNLKMIGSVIHE
                     KDLSRLRPEYLRGL"
     gene            3499..4332
                     /locus_tag="PLG98_01065"
     CDS             3499..4332
                     /locus_tag="PLG98_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE31869.1"
                     /translation="MNIKKNISKLFFWREQKYIKNKINAMLSVAQKSGLVDASSREMI
                     ENILEFSHTLVREIMIPRTDIVAVSTEAAIDDIIHEVIASRHTRIPVYRDTIDNIIGI
                     LNVKDILMFWSKPMTKEDLIACLSTPHYIPETKNTHLLFYELKENKKHMAIVIDEYGG
                     TAGLVTLEDMLEEIVGEIRDEHDGNVTDDRIIQSPDGSLIFDGRMEIEKIEENLNIDL
                     KKGRYETLGGLILDAIKRIPVSGEKFQIEGLDVTIDKADERSIKKVKIKKIETDNDDE
                     K"
     gene            4304..5884
                     /gene="lnt"
                     /locus_tag="PLG98_01070"
     CDS             4304..5884
                     /gene="lnt"
                     /locus_tag="PLG98_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOE31870.1"
                     /translation="MKPTTTMKNNFPEVSLADISRNLFRKNIFFAALSGILLFLSFPK
                     YGSGVLAWVALVPLFYALKNAATIRHGLLLGFITGIISYVGIIYWIAHVIVNYGHLPL
                     HIGIALMLLLAAYLSLYLSFFAGCLVSFREKVPFYIIGPALWVSLEFCKSFILTGFPW
                     ENLGYSQYGNTYLIQVADIFGVPGLSFLIVLVNAVLLDVIHRRTKKEYVAAGMVFVLI
                     AGIYVYGFFRLREIDATVTNAPGIDVSLIQGNIDQAVKWDQDYKQETLDIYERLSLKN
                     KPGRGSLIIWPETAVPFKFQNINRFRRQIIDLSLKTQSWLLFGSVSYEPGAYGNDFFN
                     SAYLLSPSGDVRGRYDKVHLVPYGEYVPFRDLMPFVKGLTSGIGDFSSGAGFDPLSLN
                     DKKIGVLICYEGILPFASRAYKNKAAELLVNITNDAWFGATSAPFQHFSMAVFRAVEM
                     RLYLVRAANTGISGIIDPAGRIIAKTGIFQEEALKGHAKFVTIPTVYERVGDIFIIGC
                     FMMVSICFIFSLRRRSQK"
     gene            5881..6988
                     /gene="prfB"
                     /locus_tag="PLG98_01075"
     CDS             join(5881..5952,5954..6988)
                     /gene="prfB"
                     /locus_tag="PLG98_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOE31871.1"
                     /translation="MTTENILETISDLKRRIQHIGECLDVPELKMKIKDLEELSLKKD
                     FWNDQDYARSVLQKKTNLSEKVERWDRFSKDISDIENLAEMAVREKDEHVLQDIDEEL
                     EQLNAAVAHEELKMMLNGEQDAMNAIVSIHAGAGGTEAQDWTEMLLRMYLRWAEKKKF
                     AARIIDYLPGDEAGVKSVSFTLEGEYAYGYAKAEIGIHRLVRISPFDAGARRHTSFAS
                     VFVYPEVDDDIKIEINDDDLRIDTFRAGGKGGQHVNKTDSAVRITHVPTGIVVQCQNE
                     RSQHQNKAMAMKFLKSRLYERELQLKNQKIDEENKQKKDIAWGSQIRSYVLHPYKLVK
                     DHRTNVECGNAGKVLDGDIDEFIQAYLMMTTTTS"
     gene            7158..8678
                     /locus_tag="PLG98_01080"
     CDS             7158..8678
                     /locus_tag="PLG98_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOE31872.1"
                     /translation="MTGLTRNRLIGLIALICLFIVPLIGYAENSIDKKSPESSGDITK
                     QKIDQKIKLAALNNDDESSLFDFPQENDPLADVQEDDEKQDMMENALELLGEADKLWK
                     NGDIEETLDTLDEAYSLILNANGDSAIAQEKDDLRLLISQRILAVYSSKRDVLQGKNG
                     EIPLLMNGDVQKEITSFQTVERDSFLAAYERSGMYRSEIVKELKRSGIPEEFFWLPLV
                     ESLFKINAYSSARALGLWQFIPSTGYKFGLNRDEWVDERMDVQKSTKAAIAYLKELHG
                     MFGDWLTALAAYNCGEGRVLRVISRQRLNYLDSFWDLYRQLPNETARYVPRFLATLHI
                     VNNPQKYGFDLSHTARPIEFETVRVNRIMKFNDIAERIDTTEDILCLLNPELRHKMTP
                     DREYDLKVPGGMLDKFSFVYHEIPSSEKPSIASVQTYVKASDRTRRTYIKHRVKRGET
                     LYSISRKYKVSISSIRSLNKLSPKKKLIQGRRLLIPVASAKRHAKGLTVKKKFKKK"
     gene            8684..9085
                     /locus_tag="PLG98_01085"
     CDS             8684..9085
                     /locus_tag="PLG98_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOE31873.1"
                     /translation="MGRYKVKKGENLSTIACRFSVSVSELKAINNLSSGQIYAGQRLK
                     IPQKEDGKDSGNEDSEKNLRTSAQKSKIDKKILSAKDINQLGPDKYIVTKNDNLHTIA
                     KKNNTNAARIMELNGLSVDAKIVPGQILVVQ"
     gene            9273..10136
                     /gene="panC"
                     /locus_tag="PLG98_01090"
     CDS             9273..10136
                     /gene="panC"
                     /locus_tag="PLG98_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOE31874.1"
                     /translation="MNVVKSVQKMQSFSENLRLEGKKIACVPTMGYFHEGHLALMREA
                     RKQADCVVVSIYVNPTQFGPQEDFSKYPRNFDRDMKMAQNVGVDVIFYPSNRAMYPPH
                     YQTYVAVEKVTQNLCGASRPGHFRGVTTVCNKLFNIIKPHVAVFGKKDFQQFVVVQRM
                     VRDLNMDLEIIGYPTVREADGLAMSSRNKYLSAEQRTSALVLFKSLQLAQKLYAKKET
                     SPRIIIDAVKKMIQKTPLTQIDYVKICDTTRLQDIAKITDQTVIALAVRIGSTRLIDN
                     HVFGEALKISY"
     gene            10161..10517
                     /locus_tag="PLG98_01095"
     CDS             10161..10517
                     /locus_tag="PLG98_01095"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOE31875.1"
                     /translation="MQRFMLKSKIHRATVTDADLHYEGSISIDEKLLEAASILPYEKV
                     AIYDVDNGERFTTYAIKGKRNSGIICLNGAAARKVAKGDLIIIASYVVVEEAETKNWK
                     PTCVFVDGKNKIKKKH"
     gene            10713..12137
                     /gene="ahcY"
                     /locus_tag="PLG98_01100"
     CDS             10713..12137
                     /gene="ahcY"
                     /locus_tag="PLG98_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOE31876.1"
                     /translation="MAFLEIDPALKYKVADIALADWGRKEIKLAENEMPGLMALRKKY
                     GQKKPLKGMKIMGSLHMTIQTAMLIETLKTLGAEIRWASCNIFSTQDHAAAAIAKKGS
                     AAVFAWKGESLEEYWWCTEQALTWPDGSGPHLIVDDGGDATLFVHQGVKVERDPALLK
                     QKIDNKEFKIILDRLEKSFRRNPKKWQKIASTIKGVSEETTTGVHRLYQMQAAGELLF
                     PAINVNDSVTKSKFDNLYGCRESLADGIKRGTDIMISGKIVVVCGYGDVGKGCAQSMR
                     GFGARVIITEIDPICALQAAMEGYEVRTMEEVAPIGDIFVSATGCCDVITGRHMEMMK
                     NEAVVCNIGHFDSEVDMHYLETSKVCRKETIKPQVDKWTLKSGRSIIVLAEGRLVNLG
                     CATGHPSFVMSNSFTNQCLAQIELATKKHKIGVYTLPKVLDEEVARLHLERLDARLSK
                     LTKKQADYLGITVDGPFKPDHYRY"
     gene            complement(12172..13251)
                     /gene="lptG"
                     /locus_tag="PLG98_01105"
     CDS             complement(12172..13251)
                     /gene="lptG"
                     /locus_tag="PLG98_01105"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOE31877.1"
                     /translation="MKLLDKYILKEFLKFFTITFLAFIALFLIVDFFERIRMFLSHQA
                     TAAQMASYFIFSVPMIISYVLPPSILLATLMTFSSLTKFSEITAMKANGINIFRMALP
                     IVMFASLTAVFYFYFTELITPAAVQKTTYIKNIEVKKQKTPGFFKQNEIWYRSDNAIY
                     NFKMFDIGKNILHGVMITYLNKDFTLISRIDARRAEWKNGRWVFHDLLTTRFDDNRAP
                     VLERSKEKIIPLPEKPEDFKVMQKDVEKMGYFELRRYVQKIQSEGFDARKYNVDLHGK
                     TAFPFVILIMVLIAVPFALRSERSGGVMQSIGIGIFIGFSYWIVHAFCMSLGKSEMLP
                     VVLAAWAANILFGAAAAFLLYKVNT"
     gene            complement(13303..14418)
                     /gene="lptF"
                     /locus_tag="PLG98_01110"
     CDS             complement(13303..14418)
                     /gene="lptF"
                     /locus_tag="PLG98_01110"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOE31878.1"
                     /translation="MRKIIDRYIFKEIAFPFILILCVLTFVLLMGKILQIMDLFINKG
                     VSIFSIAKIFIFMLPYFMLFTIPIALLISILIAIGRLSSDNEITALKTSGVSMARIYY
                     PVAVASLLAFVFTLLVSYFVAPQSNFATKRLLFDIAQQNASVGIKEKVFNDSFKGFII
                     YTNKISEGNHALEEVIVSDKRTIGEQNTIIAPRAFLVSDPDTMRVKLRMENGSIHTVS
                     SDLKNYRKIDFQSYEINLDLYAALAEFDKASKAAKDMTMTELLQNMKKPGMSEKDVRE
                     LSIEAHKKFSIPLACIFFGLLALPLGMRSQRSVKARGFAVGLITVCLYYLLRIGGEAL
                     AETGYIPVAVGVWIPNISFAFIGIFMFYITSKEIYLF"
     gene            14722..15957
                     /locus_tag="PLG98_01115"
     CDS             14722..15957
                     /locus_tag="PLG98_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOE31879.1"
                     /translation="MKVDTMRRIDFYAGVPLCFFGSLFYKAVKWLSPRTKKDPKNVLF
                     IELSEMGSAILVDPAMRKLRDTTGAQLHFAIFKKNKPSLDLLQTVSPDNIYTIREDRL
                     SSFLMDTFKFLHWTRKRKIDTVIDLELFSRFTALLTGFSGASNKAGFHAYFNEGLYRG
                     DFLSHKVAYNPHMHIAKNFIALVNALLSPKEEIPYSKTRIQDAEISMAKVHITDTQKK
                     TVLDIIRGCFDAFDPSRHKIVLINPNASEMLVQRRWPQAYYGHLIKMILGQCSHAVVL
                     ITGDPREREEAERLKEQVQDERCVNFAGKVSFAQLPQLYSVSEFMVTNDSGPPHFAAI
                     TEMPTYVIFGPETPALYGSLGRTTPIYAGLACSPCVSASNHRKTACTDNVCLKVITSS
                     QVFETIRPVLDRINAETLL"
     gene            complement(15969..16259)
                     /locus_tag="PLG98_01120"
     CDS             complement(15969..16259)
                     /locus_tag="PLG98_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31880.1"
                     /translation="MMRRCHKCLNEISDNVYTGRQSQCPVCSADLHCCLNCSFYDAGA
                     YNDCREPQAERVLDKARSNFCDFFRFRQTETLSDAPSQNTPGDKLEALFKKS"
     gene            complement(16357..16719)
                     /locus_tag="PLG98_01125"
     CDS             complement(16357..16719)
                     /locus_tag="PLG98_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31881.1"
                     /translation="MTNDKEYRLSASINDGIIEIILSGTVTRTNVSRLQKEIDSLRNE
                     KGSRLLIDVRTLNEGSGYHDVLYYIRRPDKATGKTAILDVPGNDRLETFLKTATLNTH
                     LEARYFSNLDLARNWLKT"
     gene            complement(16949..18181)
                     /locus_tag="PLG98_01130"
     CDS             complement(16949..18181)
                     /locus_tag="PLG98_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOE31882.1"
                     /translation="MNPTQPSFLKSYGFSILLIASIVIGAVLGFVFKADAVRLKPLGD
                     IFLNLIFTAIVPLVFFSIASAVAGMQNIRRLGKIMSAMIFVFIATGLIASAVMIAAVI
                     FFPPAAGLNIGLGNGVDVEHLKASDQIVKAFTVGDFTELLSKKNMLALIVFSVLTGLA
                     ASVTGERGKPFANFLNSGSAVMMKVISFIMYYAPVGLCAYFAYLVGVFGPQLLDSYAR
                     AMMVYYPTAFLYFFIAFSLYAFIAAGIDGMRRFWKNIIPPSLIALATGSSMAAIPANL
                     EAADKNGVPRDIREIVIPVGATMHMEGSCLAAVLKIAFLFGLFNMPFSGAETLLTAFG
                     IALLTGVVVSGIPGGGTIGELLIISFYGFPMEAFPIITMIGTLVDAPATMLNAVGDNV
                     SGMLVARILDKKNPVAPD"
     gene            complement(18186..18953)
                     /locus_tag="PLG98_01135"
     CDS             complement(18186..18953)
                     /locus_tag="PLG98_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOE31883.1"
                     /translation="MKIRFLLAVTVSFSFFYFASAAHCSDLNKKPDRFVDIQTVIPGA
                     LLDIRYHGAHNFLGERVDGYLAPKCYLTKEAADALALVQKDLEAYSLTLKIYDCYRPQ
                     RAVHHFVRWAKDIENTKTRKEFYPTVDKRNLFTDGFIADRSSHSRGSTVDLTIVLLSA
                     PPQPDYTPGQTLSECYLPSGKRFADNSIDMGTGFDCFHELSHTANAKIGEQQKINRLL
                     LKSLMEKHGFKNYDLEWWHYTLKNEPYPDTYFDFIIE"
     gene            complement(18974..19567)
                     /locus_tag="PLG98_01140"
     CDS             complement(18974..19567)
                     /locus_tag="PLG98_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOE31884.1"
                     /translation="MSRPHQPDGSSQVLLVQRCGFWIFTRTIVYVMEQREGNWSAVSP
                     PMDAVIGRNGFAPPGEKREGDGKTPSGIFPLVMAFGYADSANTKMLYRQIKEDDIWVD
                     DSEAADYNRWTKQSVTQSTSYERMKRDDDLYKYGIVIAYNTNPVVQGRGSAIFVHVWK
                     DKSVPTAGCVAVSEENILRLLHWLDPAAAPLIIMGLK"
     gene            19863..20759
                     /locus_tag="PLG98_01145"
     CDS             19863..20759
                     /locus_tag="PLG98_01145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOE31885.1"
                     /translation="MSDNFFTFMIIPNRKSGVKKISVPKVAIRNILIGAIVVVLIALF
                     VIYDYASIKRDRAELARLRAQTKEQSRQLQDMGMKIDQFADRLEALRQYDKQIRVLAH
                     ETNLSKKMPLGVGGSEEVRMKDLVDKDHEQIIAGMRRNFEKLHEEANERERSFTELLG
                     YLGQQKAVLASTPSLWPVKGWVTSEFGMRRSPFRSGMEFHKGLDISTRFGKEVVAPAD
                     GLVISSGYGPQDGNYVKIDHGHGFTTAYLHLSRMTVKQGTRVNRGDVIGYVGDTGRST
                     GPHLHYAVLVNNVEVNPRKYLR"
     gene            20870..23398
                     /gene="secA"
                     /locus_tag="PLG98_01150"
     CDS             20870..23398
                     /gene="secA"
                     /locus_tag="PLG98_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOE31886.1"
                     /translation="MLDNILKKIFGTKNDRELKRLSLLLREINNLEPAMTALNDEELK
                     AKTPYFREKLKNGFSLDDILTEAFATAREASRRTLGMRPFDVQIIGGIALHEGKIAEM
                     KTGEGKTLAATMPLYLNALEGKGCHVVTVNDYLARRDAEWMGPIYKFLGLTVGVIIHG
                     LDDPERREAYHCDITYGTNNEFGFDYLRDNMKFTLDDYVQREFNYAIVDEVDSILIDE
                     ARTPLIISGPSEESTDKYYKINQIIPRLKKDKDYSIEEKSRTVVLTEDGVAHVEKLLN
                     VQNLYEPRNIEIVHHVNQALRAHTLFKRDVDYLVKDGQVVIVDEFTGRVMPGRRYSDG
                     LHQALEAKENVKIERENQTLASITFQNYFRMYKKLAGMTGTADTEAAEFKKIYNLDVL
                     VIPTNMPMIREDHNDLIYKTEREKINAVIGEVKTLNKERRPVLIGTISIEKSEMLSKH
                     LLRAGIKHHVLNAKNHEKEAEIVAQAGQAGMVTISTNMAGRGTDIKLGEGVAELGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDRGSSRFYLSLEDDLLRIFGAERISGIMDKIGIE
                     EDQPIEHKYISRAIENAQKRVEGQNFDIRKHLLDYDDVMNRQRKVIYEQRKKVLRGDN
                     LWADTEEMIEEIIDNILSEFVDEKTKKEDINFKGLDDALLKQFNIRFDLANTDQTGDG
                     QSAIREWIIQGVQKYLRDKEKEFGPELMNYLMKVIMLQAIDVQWKEHLLAMDHLREGI
                     GLRGYAQKDPVREYQREGYDMFMEMIARIKMDVLEKLCLIRIQREEEVEAIRQKQKQD
                     YVMSRGEDTPEKKTVKHEGPKTGRNDPCPCGSGKKYKKCCGASV"
     gene            23417..24616
                     /gene="argJ"
                     /locus_tag="PLG98_01155"
     CDS             23417..24616
                     /gene="argJ"
                     /locus_tag="PLG98_01155"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOE31887.1"
                     /translation="MIKNKLKISVPGFLAGGISVGIKKKDQKDLGLIYSTVPARAAAM
                     FTKNTFKAAPLLINEDRVKRGIAQAIITNSGCANAATGKEGIRDALIVSGALSRELAI
                     REELILLGSTGVIGKRLPVKKIEGGIGKLVKGLRETGIEDAEKAMMTTDKYPKIAIRK
                     GVIGAKDITLCGIAKGAGMIEPNMATLLTYVMTDALIDYRALNTVFHQAINATFNSIS
                     VDGCMSTNDMAIILANGLAGNNPLEKVQARLQLFRDMLTEVLMELCQAVVKDGEGATK
                     FISIMVKGARSKSDARCAAYAISNSNLVKTACFGEDPNWGRIIAALGASGISMDKEKV
                     VLTIADLAVFSQNTPQEVLMSRLKDAFKKDRIDIEVDLGAGDKSYYVYTSDLSHEYVK
                     INAEYST"
     gene            24804..25619
                     /gene="rpsB"
                     /locus_tag="PLG98_01160"
     CDS             24804..25619
                     /gene="rpsB"
                     /locus_tag="PLG98_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOE31888.1"
                     /translation="MSAISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNNIYIIDLQ
                     QTVGLFQSAYNFVIDTVSEGKEILFVGTKKQSQEAIKEEATRCGMPFVNQRWLGGMLT
                     NFVTIKKSIDRLNSLTDMFNDDSIKAFPKKEITKLQKEKEKMENVLGGIRTIKSLPAA
                     VFIVDPGKEHIAVNEAKKLRIPIVAIVDTNCNPDDIDYVIPGNDDAIRAIKLFTSKFA
                     DAVLEGKKRFEEKLAAESNKEADAAVETKPVEADIPESLEIDDSIDKSETYAE"
     gene            25669..26274
                     /gene="tsf"
                     /locus_tag="PLG98_01165"
     CDS             25669..26274
                     /gene="tsf"
                     /locus_tag="PLG98_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOE31889.1"
                     /translation="MEITSTMVKELRTKTGAGMMDCKQALAESDGDFEKAIDFLRKKG
                     LSAATKRSSKAAKDGTIASYIHMGGKIGVMVEINCETDFVAKTEDFQTMARDIAMHIA
                     ASNPLYVRPEEIPESALEREKEIYRSQLREEKKPEKIWDKIIEGKLKKYYEDVCLTQQ
                     KFIKDTDITVATLINNMIAKTGENMVIRRFARFQLGEEIKQ"
     gene            26274..26999
                     /gene="pyrH"
                     /locus_tag="PLG98_01170"
     CDS             26274..26999
                     /gene="pyrH"
                     /locus_tag="PLG98_01170"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOE31890.1"
                     /translation="MSTKQKAAYKRVLLKLSGEALMGKQSTGVDPDVANYIADEIKSL
                     SRMGVQLGIVIGGGNIFRGIEASARGMDRTTADYMGMLATVINSLALQSALEERGLPT
                     RVQSSIEMHEIAEPFIQRRAIRHLEKGRIVIFAGGTGNPYFSTDTAAALRAVEIRADV
                     IMKATKVDGVYNADPVRDKKAVKFNKISYIDVLTKNLKVMDSTAISLCRDNLLPIIVF
                     NLQKKGNICKAVCGQKIGTYVGE"
     gene            27002..27559
                     /gene="frr"
                     /locus_tag="PLG98_01175"
     CDS             27002..27559
                     /gene="frr"
                     /locus_tag="PLG98_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOE31891.1"
                     /translation="MKETVFQKFKEDMEKTIAGLSKSFSRIRTGRASVSLLDGIKVDY
                     YGAPTPIAQVANVSVPESRLILIVPWDVSIIGAIEKAIQKSDLGLTPANDGKVVRLSI
                     PQLTEERRKEIAKNVKKMAEEGKVKLRNARRDANEELKKLKKDNKISEDELFTAQDEV
                     QKLIDQYIDKTDKILAAKEKEIMEI"
     gene            27738..28472
                     /locus_tag="PLG98_01180"
     CDS             27738..28472
                     /locus_tag="PLG98_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOE31892.1"
                     /translation="MVKIDVNKLPRHIAIIMDGNGRWAKQHAMGRIRGHKKGAEAVRT
                     TVRTCREIGVKYLTLFAFSIENWERPDKEVKALMYLLEEYLNAELDELQKQEIRLTTI
                     GETDRLNETVREKLIETIDRTAQNDKMVLNLALSYGAKDEILAAVKTVVRDAQTGSLD
                     IDRLDKETFSRYLYTAQMPDPDLLIRTSGEYRISNFLLWQMAYTELYFTDVLWPDFTR
                     EDLLKAIASYQKRERRFGLTSEQLQE"
     gene            28510..29328
                     /locus_tag="PLG98_01185"
     CDS             28510..29328
                     /locus_tag="PLG98_01185"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE31893.1"
                     /translation="MAKTPSSLIKRWLTGIVLVPLLLLVIFFGSEAMFAAVVILFICG
                     GVWEYHHIVFGQGFVMEKIESLAFAVLIPLCVLSGNHQYLTALLALAVMLVFVLFIGS
                     IRESSFDFLSVAKVVFGIMYIPFLMSHFIAIRMLDQGVWWIMFVLVLAFAGDTVALYT
                     GKYFGKHKLVPLISPGKTVEGLIGLVVGSTVACLIYSHYLLPDVAVVHVAVMAFVGGM
                     IGQLGDICESAIKRGYGLKDAGSILPGHGGILDRLDCLMFIAPFVYYYRIFVIG"
     gene            29329..30492
                     /locus_tag="PLG98_01190"
     CDS             29329..30492
                     /locus_tag="PLG98_01190"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOE31894.1"
                     /translation="MKKITLLGSTGSIGLSALDIIEKNPKRFRIAALAAGENIQLLKR
                     QIERFKPEMAAVKNKETAVKLRRALRPGTRIRISYDTEGLQETAACSSADIVISAISG
                     AAGLLPTLAAIEAGKDIALANKETMVMAGRIVTRAAAKKNVRILPVDSEHSAIFQCLE
                     GSRKEDLKRIILTASGGPFLNVAARQLKKVTLRQALRHPRWKMGKKITIDSASMMNKG
                     LEIIEARWLFNTSFDQIDVLVHPQSLVHSMVEFIDGACLAQLGVPDMKIPIAYALTYP
                     ERIANDLKILDLVQAGRLDFYKPDLKKFPCLKIAREAGVSGGTAPAVLNAADEVAVAA
                     LMQERLSFADLPKIIEKVLDAHDVIPDPSMDDILQADADARQQTEKIIGRITT"
     gene            30489..31580
                     /gene="rseP"
                     /locus_tag="PLG98_01195"
     CDS             30489..31580
                     /gene="rseP"
                     /locus_tag="PLG98_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOE31895.1"
                     /translation="MTTLISFIILLGILIFVHELGHFLVARMSGVGVQKFSLGFGPKI
                     IGRTIGETEYVLSWIPLGGYVKLMGESGTEELSPEDEKRSFARQNVWKRIMIVAAGPA
                     FNFLLAVVIFIAVMMYGLPTLTSDIGEVQKDSAAYQAGMLNGDKIISLDGKKIALWEE
                     IRDVVARAKDRQIEAVVQRGKETVRLQITPKLSKSKNLFGEDVSAYLIGVSPAGQTVI
                     ERKSPVDAVVSSVAKTWEISKLTVISVIKIFEGVISPKTLGGPIFIAQVAGAQVREGI
                     IPFVLFMAILSINLGVINLFPIPVLDGGHILFYLIEVVTRREVSAKVREMSQQIGFVV
                     LLMLMLFVIFIDIERLNIKFVNDVVKFFK"
     gene            31582..32265
                     /gene="tsaB"
                     /locus_tag="PLG98_01200"
     CDS             31582..32265
                     /gene="tsaB"
                     /locus_tag="PLG98_01200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOE31896.1"
                     /translation="MRILAFDTSSQTASVAVLQDDDILYDVIIHTGLNHSETLLPAID
                     QACLRTKIPLPEFDVFACTLGPGSFTGLRIGAGTLKGFMLATGKPAVGVSSLAAIALN
                     AEKTSKKIYALMDAGRGQVYTACFQYGAGGMIEQQGGEQVANPSDIVYDADAVYVGSG
                     VIKYGAAFMPERQNGILSTAKQHCRASAVAVLGKAKYERGERLNAENFVPFYLRSADA
                     DQKKSFFGK"
     gene            complement(32279..33100)
                     /locus_tag="PLG98_01205"
     CDS             complement(32279..33100)
                     /locus_tag="PLG98_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE31897.1"
                     /translation="MNIANSKNINKDNYSPSDYENWGFIYLENKEFDQALKCFQKTIE
                     LEPHNPKNYIHLGYVYEKMDMLDSAKKSYEKALEVKSDYIEAILKIGHILEMQGDYHN
                     AVELYTRAIDIAPQAIEPRFCLATLYDRHDMYDEAAEEYENIIEINPKHTKAIHNLGR
                     IYFQDGDYARALKSFQKVLNIEPENTDAWNDLGSVYEITNNLIQAISTYRKALKVNPL
                     HEDTNYNLANAYFSLFLSNPNIVKIDDIVERLQLVLSQNPNNRKVWELLSKIKAS"
     gene            complement(33178..35205)
                     /locus_tag="PLG98_01210"
     CDS             complement(33178..35205)
                     /locus_tag="PLG98_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE31898.1"
                     /translation="MYCDYWNLTKPPFDNVPDASMYVDCHESMENVISETLFAIKEGN
                     ECLTVIVGEVGLGKTLSLRVIIDSLEPEKYKVALITNPALSFVQLLREIIGQITGKQC
                     EVKNKVDLLEIFNRLLFETADQGRKIVIVIDEANVLSPSNLDDLRLLTNMQDDERNLF
                     TLLLVGQMEFAKTLEHPKRANLFQRIGTYGHLEKLPSPEAVRTYIESRLRIAGTDRKI
                     FDDDAIPVIWEFSEHGVPRLINKICKLCIKAGETNEFQTISGDVAQQIADRFQKLSKT
                     SAQKRKAPAAEAQPAAEAPRKQEPERIPKPSRPAFPEKETPAVSAPVRPAPPRMEEPA
                     PMRPRMAPPEQPPARPTAPPVIDEAPPVRPRPVMPEQPPMRRPLHPGMEDLMPPRSRM
                     APPEQPPVSPALPSMEEPAPMRPRMAAPEEMPLRPTAPPVMDEAPPARPRPVMPEQPP
                     VSPAPPRMEEPAPMRPRMAAPEEMPLRPTAPPVMDEAPPARPRPVMPEQPPVSPAPPR
                     MEEPAPMRPRMAAPEEMPAYTAPVRQEEPIPVRPQAVPVPPKPSASVVPPPVSAPGNV
                     APPQGVRAADTAQQSYEEQPMPMEAVEEEDFEEVTIGQQSIKLAIPSHVIRQAQSSNK
                     ENKNKLAGYWSAQILKGNPQLTHSSFADPVSLWFEVKNVILKKFNSPAHMQ"
     gene            complement(35275..35901)
                     /locus_tag="PLG98_01215"
     CDS             complement(35275..35901)
                     /locus_tag="PLG98_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE31899.1"
                     /translation="MEKICIVKRRREYTQPEVKVTVTQPAISETAAPEVAVSRVDVKN
                     NAGLSENTARTTKESMTPELTIRSVGMEHEIKIPERKIVEEFVDNASSVIAITMTKEQ
                     SDLLLQSEYIKELLSGKKSDPALDIKVTPEGRLSLNYRFSDSLLLRMIAPNQVCQMLQ
                     VSRSFLQKLVHENKIKSYKLGRMRRFLLEDILEYLSSEVDFMQPSDDK"
     gene            complement(36221..37915)
                     /locus_tag="PLG98_01220"
     CDS             complement(36221..37915)
                     /locus_tag="PLG98_01220"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31900.1"
                     /translation="MKTLLSLIGPRVRSSLNSLTRGNRKNVWSRLVMFGGLGLLFWAG
                     IFMIFYRVLLYFHSVQDFGDILAMKFVSMIILTFFMLLLFSNIINCLSHLYLSQDLPL
                     LHSLPVSANDIFFSRWIISAFDSSWMILAFSLPVFLSYGIIFHTGIFYYAIFTTVMIF
                     MCLIAAALSGILVLFGSKVLPAGRIRTILILLGALMMIVLIFVLRFTRPEQLVNPDSF
                     ASVVLYLNALKTPSSPVLPTTWITDAILAALKNDRATALLNAGLVVTFACMLVFISSM
                     LARNMYFPGFSKSQMTPRRLFAPLRYKGFSWETLLNFLPRESKAFAVKEIRTFFRDSS
                     QWPQLFLMTALIVIYIYNFSVLPLESYPIKTIYLQNVFSFLNIGLAAAVLAAVAARFV
                     FPAVSMESEAFWIVQSAPVSIGNFLWIKFFLYYIPLMMMAEGLVVASNILLNVSDFMM
                     LLSVLTVFFLVPAIVGLATGLGAVYADFKSENPANIVTGFGGLLFMILSFGLIALVII
                     LEAGPVYTIFMADVLGKNLTALKILWAVLSFALAIFLCLLATFYPIHLGRKRLTVK"
     gene            complement(37912..38646)
                     /locus_tag="PLG98_01225"
     CDS             complement(37912..38646)
                     /locus_tag="PLG98_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE31901.1"
                     /translation="MITLSELTKDYGTTVAVDKLSLTVNAGEIYGFIGPNGAGKTTTI
                     RMMGGILAPTSGSIVIGGVDMARNPVEGKRMIGFVPDRPFLYEKLTGMEFLEFSANLY
                     NVRRGLFPEKAKRLLQQFALWTWADELIEAYSHGMKQRLIIAAALLHDPQILIIDEPM
                     VGLDPEAVHMVKDILKDLAARQTTIFVSTHTLSIAEDLCHRIGLIHKGTLLAEGSLKE
                     LKQIARLGEARLEEVFLTIIKENTFL"
     gene            complement(38643..40118)
                     /locus_tag="PLG98_01230"
     CDS             complement(38643..40118)
                     /locus_tag="PLG98_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOE31902.1"
                     /translation="MTRPFKWPMAAGILVTIAFAVLLLIRLDFFQTQTPPVAGIKMVP
                     PQQPQDIWMTIFQNDRKIGFVHRMLTKNENGFAFNENVFMEISTMGMPQALNLATEGQ
                     LNADMTLSSFTFKLNSSLFQFHIRGRVADNKLILYTGAPNRQQKNEMPLQALPHISGN
                     IYDAAFHAGMDKGTTRGFSIFDPSSMSVRTINVTRNADEIIPIMGNRLLLKKYCADFM
                     GAKHCAWLDKDGQVLKETGMLGLSMEKTSRENALAEIDGSAGVDFTQIASIPSNVEIA
                     DPEKLRKLQIRISGITPPLQRQLHLDGDRQHYHDGVLTITREDPADKGPAGGWPADIA
                     VFLKPAALIQSDDPQIKALIGKIVRPEDSAFVKARKIINWVFLNIEKKPALSVPNALE
                     VLKNRSGDCNEHAVLTVALLRAAGIPARSEAGLVYLRGRFYYHAWCVLYINRWITADA
                     VFNQIPADVTHIRLLRGETDQQIRLIGVIGKTNLEVLSQTR"
     gene            40374..41018
                     /locus_tag="PLG98_01235"
     CDS             40374..41018
                     /locus_tag="PLG98_01235"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOE31903.1"
                     /translation="MKHDSIIAAEGYPFVVFSLIVTVFVAFLGGNWWLILFFALVTVF
                     ILCFFRNPERSFPEDEKVLISPADGKIIKIEEVDVNGTLSGKFKKISIFMNVFNAHVN
                     RAPYDGKIEAVHYHPGKFFSANLDKASEDNERNEILIRTPDNRTVWAVQIAGLIARRI
                     VCWVTPGMPIRKGERIGIICFGSRVEVYLPVDSAISVKLKDKVKAGQTVLGYLS"
     gene            41015..41797
                     /gene="pssA"
                     /locus_tag="PLG98_01240"
     CDS             41015..41797
                     /gene="pssA"
                     /locus_tag="PLG98_01240"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOE31904.1"
                     /translation="MNVENAARKIRMKKGIYLLPNLFTSASLFCGFYSAIASFKEHFV
                     TAAIAILVSVIFDGLDGRVARLTNTTSKFGAEYDSLADVISFGIAPALLAYSWSLSVY
                     GKWGWVVAFLFVLCGALRLARYNIQIGIIESKVFNGLPIPAAASVIATTVIFFDYVGA
                     EGKFHNIFIMVFVIMISLLMVSSVKYYSFKDMSLLVRKPFTIFFWSTVLLIIIVAEPE
                     IMFFVIILGYALSGPVWWLIKFGFGKPKAVPENKNNAANGKP"
     gene            41834..42391
                     /gene="rsmD"
                     /locus_tag="PLG98_01245"
     CDS             41834..42391
                     /gene="rsmD"
                     /locus_tag="PLG98_01245"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOE31905.1"
                     /translation="MRITGGKARGKMLLFPSGSKERPTTDFLREALFNLTGSLNEKTF
                     LDLFAGSGSVGLEAASREARQIVLVENNQKLAGVIRKNIPNGLQDGRLTIMATDVAFA
                     LRDLFRKQCSFDIIFADPPYHRGLVAVTLELLGKYPVLAKGGILVMQHSVKEPIPETV
                     LGIFHRTDQRKYGENALTFFKMERE"
     gene            42391..42894
                     /gene="coaD"
                     /locus_tag="PLG98_01250"
     CDS             42391..42894
                     /gene="coaD"
                     /locus_tag="PLG98_01250"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOE31906.1"
                     /translation="MQEDFTKRIAIYPGSFDPITNGHVDIIKRGKRMFDEIIVLIAYN
                     PNKVYLFNIEERMEMIREIFGETPGIRVDCYSGLLVDYLKMSGANIILRGMRALSDFE
                     YEFQMALMNRRQTKHIETVFLMSGFKWFFTSSKLIKEVASLGGSVKGLVPDNVYQRLT
                     EKYNTRG"
     gene            42898..44091
                     /locus_tag="PLG98_01255"
     CDS             42898..44091
                     /locus_tag="PLG98_01255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE31907.1"
                     /translation="MRLSARVGRISPSETLAITAKVNALRAQGRDVIGFGAGEPDFDT
                     PDNIKDAAIKAIQSGFTKYTPVGGTDELKDAIINKLKRDNRLEYKRSQIVVSCGAKHT
                     LYNLAQIYFDAGDEVIIPAPYWVSYPDIVTLADGTPVIVKTEEKDAFKMKPEQLAASI
                     TARTRALILNSPSNPTGAAYSPDELKAIAQVLLDKDILIITDDIYEKIFYAGFPFANI
                     ANVEPKLKDRVVVVNGVSKTYAMTGWRIGYAAAAEEIASAISKIQSQNTSNPTSIAQK
                     AAVEALSCDQTMVGDMAAEFKKRRDYIVAALNEIPGISCFSPEGAFYVFPNVSGIYGR
                     SCGGKVINGSADFIDYLLDEANVAGVPGAAFGSDSHIRLSYATSLKNIEEGIRRIREA
                     VSKLT"
     gene            44108..45511
                     /locus_tag="PLG98_01260"
     CDS             44108..45511
                     /locus_tag="PLG98_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOE31908.1"
                     /translation="MGGIFGVVSSANCIKTLFYGTDYHSHLGTEHAGMAVLGPQGFYK
                     TVHSVSQVQFKSRFIDEYKKMDGFFGIGAIDDDTPQPLIFRSRFGVYAVAAVGLINNK
                     NQLVNELLKEGANFGERAGGDINNAEVVSCLINRGDNIVEGIASMRAVIEGSICAVVM
                     TSEGIYAARDKFGRFPLAIGKSKNPQDQSYVVATEASSFENLGYELTQFLGPGEIVLL
                     TTDGVKPVKPAGTEKKICSFLWIYTGYPSSSYEGISVEGARERCGAYLAKRDNIQADF
                     VSGVPDSGVGHALGYAMASGIPYRRALVKYTPGYGRSYTPPAQEIRDLVATMKLCAVR
                     EVINGNRIIICDDSIVRGTQLKNLTIKKLWDNGAKEIHVRPACPPLMFPCIYTSSTRT
                     TGELACRKAIRALEGKEIEHPSEYLDHHSEKHAKMIDWIRRDLNVTSLKYLAIDDMIA
                     AIGLPADQLCLHCWLGK"
     gene            45606..46700
                     /gene="ispG"
                     /locus_tag="PLG98_01265"
                     /gene_synonym="gcpE"
     CDS             45606..46700
                     /gene="ispG"
                     /locus_tag="PLG98_01265"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOE31909.1"
                     /translation="MATVEHSSFIRRKKTKQIHLGGVAIGGDAPVIVQSMTNTDTRNV
                     EATVRQIKALEKAGCEIVRIALPDDKAAASVSAIKKRIRIPLIADIHFHDRLALEAIR
                     QGVDGIRINPGNIAPGKMGAIIRLARERRVVIRIGINSGSLQKDLLKKYGGATPEALV
                     ESAVRNLELFEDRGFDLIKLSLKSSDVPATIEAYRAIAARTDYPLHLGVTEAGSLIGA
                     AIKSSLGIGTLLYEGIGDTLRVSVTGDPVSEMGLAYGILRALGIRRVGVDIVSCPTCG
                     RCEIDLPSLTRRIEKALAGRKDYLKVALMGCVVNGPGEAADADIGLAGGKGQGLLFKK
                     GKAYKKVREKDFVNILLEEIDTIIEERKKD"
     gene            46700..48427
                     /locus_tag="PLG98_01270"
     CDS             46700..48427
                     /locus_tag="PLG98_01270"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOE31910.1"
                     /translation="MRYSEMFLPTVREVPSDAEIISHQLMIRAGLIRKLTSGIYSYLP
                     LGYRVIRKLEQIIREEMNRAGAQEVHLPMVQPAEIWQESGRWTYYGKELLRLRDRHDH
                     EYCLGPTHEEVITGLVRNDVKTYRQLPLNLYQIQTKFRDEVRPRFGVMRCREFGMKDA
                     YSFDVDEEGAEKSYEKMFAAYNNIFRRCGLQFRPVEADSGSIGGKYSHEFMVMADSGE
                     DAMVFCEKCAYAANLEKAEVACPEKKIIFEDDWLPLEDVDTPDVRTIEEVSAFLKVTP
                     QNIVKTLIFNADGKPCAVLIRGDHEVNEVKVKNYLAAAELELASDDMIREVTGAPRGF
                     AGPVNIKAPILVDYSVIDMVNFITGGNRQDGHLKNVNVGRDFKVEAFTDLRIVNAGDP
                     CPRCGQPIKTARGIEVGHVFKLGTKYSKAMKAVYLDKDGQEKIMIMGCYGIGVGRTVA
                     AGIEQNHDDNGIIWPMPLAPYQVIISPVNINEITIRETAEYLYNAMITEGVEVLYDDR
                     DERAGVKFKDADLIGIPLRVTVGHKNLQDGNVELKIRKTGANELCPLQDIVGRVREII
                     RQELNPDIA"
     gene            48455..50608
                     /locus_tag="PLG98_01275"
     CDS             48455..50608
                     /locus_tag="PLG98_01275"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOE31911.1"
                     /translation="MLRINDIIEKAQTYLPAEDLEMIEKAYIYSASVHQGQVRLSGEP
                     YLTHPMEVAGILTDMKMDAATIISGLLHDTIEDTLTTADQLEHEFGKDVSFLVGGLTK
                     LSRISFGSQEERQSENFRKMILAMSSDIRILMVRLADRIHNMRTLQYHPKEKRIYIAR
                     ETMDIYAPLANRLGINSMRMELEDLAFQYIAPDAYNDLTAKIVKSNEARNRYTEEVKN
                     IIQQEMEKYSVACKVEGRAKHLYSIYKKMHEQHINFDEVYDLIAFRIILDADKEKSCY
                     EVLSIVHALWKPVPGRFKDYIAMPKANNYQSLHTTVIGPYGERVEIQIRTRNMHEWAE
                     KGIAAHWRYKEGGSYKDNEEDQIRKLREVLEIQQDIKNPKEFMSDLKLALFPEEVYVF
                     TPHGDVKSFPKGATPIDFAYSIHTDVGNQCIGAKVNRSIVPLRYKLQNGDRVEIITQA
                     GHHPSKDWLKNVVTTRAITKIRNYINLEEKKNSIVLGRELLEKEFKRHNLKFSGIVKS
                     DDIKKIFSAFSVNQLDDLLFQVGLGRISAKRVAHYFIAGEEVEAAEPAREKIKKKKAA
                     SPSMGVSLIGIDDPDDIMVNFAKCCNPIPGDDISGYISRGRGVIVHTSNCPYIKRMDQ
                     ERIVDVQWSSREEHTYPVNIKVVCDDIKGILTEVSSIISSFDVNISYAEIETVNMIAT
                     CKFIIDISNLPQLNQIMSAIRQLKFVKAVEWLHHF"
     gene            50673..51362
                     /locus_tag="PLG98_01280"
     CDS             50673..51362
                     /locus_tag="PLG98_01280"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOE31912.1"
                     /translation="MFHTKKILVLITFAVLACFGWGAAELIAAEKKYIILIDPAHGGK
                     DRGLKLNDDVSEKDVTLAVALQIKKELAQEPNLQIILTRETDKTLDREARQKMIKTLN
                     PDMVLNLHVNGGFGKEASGYEIYYPSCGADDPASMRKTDKTKPQSQCQNNSLKMAKLI
                     SDNFNALFPRKARGLRMADLSVTEGVLVPAVAVEMGFATNAVDQKNLMSKKTQAEMGK
                     ALAVSIKAFSR"
     gene            51366..52097
                     /gene="rph"
                     /locus_tag="PLG98_01285"
     CDS             51366..52097
                     /gene="rph"
                     /locus_tag="PLG98_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOE31913.1"
                     /translation="MTERKSGRRFDELRPIKLIKNYLNTAAGSVMVEFGNTKVLCAAS
                     IDEKTAPFLRNTGRGWVTAEYSMLPMSTQTRTARESVRGKIQGRTQEIQRMIGRSLRA
                     VTDLSAFGEKTIYLDCDVIQADGGTRTASVTGAFVALVGLFDQLKRDGVVQNIPVRDY
                     LSAVSVGIIDQQVMLDLEYEEDSRADVDMNFVMTGSGHFIEVQGTAEKIPFSREQMTQ
                     MTDVAERGIRQIIERQKELVGDLIG"
     gene            52094..52690
                     /locus_tag="PLG98_01290"
     CDS             52094..52690
                     /locus_tag="PLG98_01290"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOE31914.1"
                     /translation="MKIVFASGNLGKVREIREMLSCINIELVSLKDYPDIPEIVEDGK
                     TFLENALKKARTVSERTAQAVLADDSGLQVDALGGEPGIYSARYAGEGASDDDNINLL
                     LEKLKEVPPEKRTASFCCVLVLYNTDGTYESFESRWPGRIIDDRRGDNGFGYDPVFYV
                     PELNKTAAELPPEIKNRISHRGQSFLKLKQSLGRKMKK"
     gene            52694..52770
                     /locus_tag="PLG98_01295"
     tRNA            52694..52770
                     /locus_tag="PLG98_01295"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52728..52730,aa:Pro,seq:tgg)
     gene            53020..53982
                     /locus_tag="PLG98_01300"
     CDS             53020..53982
                     /locus_tag="PLG98_01300"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOE31915.1"
                     /translation="MERFRKLCVIILSLSFIFSVAACDNFSKFKEKMLSEEIISFPVK
                     QGNSWGFIDTTGKFVIQPQFDGAGRFSDGFAAVKIGDRWGFIDKTGEYAINPQFDVAF
                     LFHDGLASVGLGGGKVKVGFINKQGKYVINPQFDDAGWFSEGLTAVGKIDGQKVKAKV
                     GFIDKSGKYIIKPRFEDAGWFSEGLAAVKIDGKYGYIDKTGKFIIEPQYDLAFWFSEG
                     MATVKNGNKYGYIDKTGKFIIEPQYDFAFWFSEGLASVKSKGKYGFIDKSGRYVIEPQ
                     FDIAFWYENGLAPVGIGRGKVKVGFINKQGKYAINPQFDGVNVF"
     gene            54109..55002
                     /locus_tag="PLG98_01305"
     CDS             54109..55002
                     /locus_tag="PLG98_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE31916.1"
                     /translation="MSGTLYPFKVNDSLWILGNGYFHLYLLKGAKQCALVETGISATA
                     DLVLSQLEALDVRPDYLVVTHPHSDHITGLDHLRGAFPGAVVMIAAGAASFVAHPKAA
                     PAIIAEDRHMFRALAARGLFSQTAVVTMPPSLSGCRILTDGEALDLGGMSVNFLSVKG
                     HSPGNLLAWIAGTKTLLVSDSLGNHYPGRGFFPTFFTGYNDYMATIDRLESFRPKRMG
                     LAHNGFFSTSGAIADIFCLSRKSAQDVRRYIEDCAMDDEDLARSLFGFYYTDELAVYS
                     PDNILNCCRLLVRRVRELHTA"
     gene            complement(55061..56398)
                     /locus_tag="PLG98_01310"
     CDS             complement(55061..56398)
                     /locus_tag="PLG98_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOE31917.1"
                     /translation="MNPHVFREYDVRGVVDKDLNEDFVFHLGRAIGTYARAKRIRNMT
                     VGRDCRLSSESYHRFLSRGLNAAGIHVIDIGMCATPMLYFSIRHLRVGGGVMITGSHN
                     PPEFNGFKICIGHSTIFGPQIQDLRKIMESGRYAKGAGRCREKDIAKEYENYLFKRIK
                     IQRRIKVVVDGGNGVGGYFAVPLLKRFGCEVIPVHCEPDGTFPNHFPDPTVEENLTDL
                     IRLVRKNKADLGISFDGDADRLGVVNDKGEIIWGDKLLLLFARHILKNHPRAAIIGEV
                     KCSQVLFDDIEKHSGKAIMWKAGHSLIKAKMKEVKAVLGGEMSGHFFFADRYFGYDDG
                     IYAALRLLEILSRTGKKLSGLFADVPKTFATPEIRVDCTDSKKAKVVEGIKQHFKNTP
                     GIVDIDGIRIPFENGWALVRSSNTQPVIVLRFEAASVKTLNKIRGEVEALLHV"
     gene            complement(56423..58318)
                     /locus_tag="PLG98_01315"
     CDS             complement(56423..58318)
                     /locus_tag="PLG98_01315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE31918.1"
                     /translation="MEPEIFEEYLAGFKKAYPKKFVSEDEIFSHIRRGDHIFVASGCG
                     EPQYLVKAMIRYVESHPKAFFDAEIMHVYSLGLAPYTEPKYKANFRHNSFFIGNSTRS
                     SINAGMADYTPISLSQVPSLLASGVVQIDVALIQTSMPDEHHYLSLGVSVDVVKAATE
                     KAKIVIAQINPNMPRIHGDGFIHIRDVDFIIPYDEPLLEVSSPKTGDVIITIGKNVAR
                     LVQDGDTIQVGWGSLPNAVMFCLSNKKNLGVHTELLSDGLVDLIKAGVINNSRKTIDH
                     GKTVASFCMGNKSTYAFLDNNPSISLRTLDYTNNPVMMSRIDNMVAINSALEIDLSGQ
                     ATSESIGGIFYSGIGGHQDFMRGALFARNGKSILALKSTSKNDTVSRIVPALKEQAGV
                     TLNRGDVRYVVTEYGIAYLHGKNIRERAMSLIAIAHPKFRPWLIDEAKKRGLIFKDQA
                     FIPGKRGEYPEDLETYLTTKTGLQVFIRPVKISDESLLKDFFYSLSDKSIERRFISTR
                     KDMPHERLQNFVIIDYTKQIVLVAIKGDVDNSYISAVGQYGIDDATHTAEVAFAVRDS
                     EQNNGIGFELLKYLTYLAKRQGLLGFTAEVLVENKPMLHVFEKGGFDIKKKIDSGVYE
                     LTLKFKL"
     gene            complement(58636..59703)
                     /locus_tag="PLG98_01320"
     CDS             complement(58636..59703)
                     /locus_tag="PLG98_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOE31919.1"
                     /translation="MKKIVVSSMRRNSGKTSIIAGIVSLMHDKKFAYAKPLGDRLIYR
                     KKRSWDYDASLMVNLLGRQGEMESHCEKITLGFDHSRLKYMYDKEGIERALAEIVTDI
                     GAENDVLFIESGKDLTAGAYLNLDAVSMTKSIDGVLVIVVSGDDDCVLDDMRFIEKYV
                     KLEGVHFGGVIINKISDIEEFEASCTPVIREMGLDVIGAIPYEEPLTYFPLDFLADKL
                     LARVLAGEEHLKNIARTILIGTSATFVADKEQIPARPELSGTGQLMITGGDRSDLILA
                     ALERDTAGIILTNDIVPHQNIISRANERGIPVLLVGTDTFQTARTIDDMEPLLRKQDT
                     GKISLLSQLIEKHTNMAEFLK"
     gene            complement(59700..61835)
                     /locus_tag="PLG98_01325"
     CDS             complement(59700..61835)
                     /locus_tag="PLG98_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOE31920.1"
                     /translation="MDNTTPYDIQYLFEPKSIAVIGASQNKKKIGYAVFNNIVSGGYQ
                     GNVFPVSPKGGDVDGHKIFSDILDIDEEIDCASIVIPADLVKDAVKKCALKKVKFLQI
                     ITSGFSEVGNVHEEKEIFDIASQAGTRIVGPNMFGLYSRAASLNSTFSAAKISPGHVA
                     ILTQSGALGIAMISKTAVANLGLSAIISIGNKCDVDEADLLAYLIQNDDTKVIFIYIE
                     GVKKGERLIEALKVATGKKPVIVIKSGKSKRGAQAAASHTGSLAGSDDIFDAIMKQCG
                     VLRAECLEDAFNWCKFMISGSAPRTRNTVIVTNGGGVGVMATDACEKYGVSLYDDQAA
                     LRGLYEAVTPSFGSTKNPIDLTGAAGGTEYTRAFSVTAESEDIGSTIGLYCETATFDS
                     GNLAQMIQETYARHQKNGKPISYALIGGKQVENAITALIAERIPVFNDVEQAVSCMGA
                     FYRYHNYLDEVSRKIDEYAVDTAAINAVIDRAAGAGRTFLLANEGAAVLRAADIAMPQ
                     SKIARSIQEAVQFAGEIGYPVVLKVVSKDILHKSDAGGVVLDLDDEKEVIVGYEAIMH
                     HCRMYKPNAVIEGIEVAEMVKKGIELIIGARRDPSFGPVIMCGLGGIYVEIMKDISFR
                     AVPINRSIALSMLEEIRSYPLLLGARGEEKKDIESVIDTIIKIGSIIRTCERITDIEV
                     NPIEVYEAKSGIKAVDARILISRMKEDTQ"
     gene            62078..>62279
                     /locus_tag="PLG98_01330"
     CDS             62078..>62279
                     /locus_tag="PLG98_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31921.1"
                     /translation="MSCRCRPASITLSVIPAKAGIRFVSFFVMSVQTGIQYSFLVPGF
                     RREDEEVTHISYLDSRQGHAGMT"
     assembly_gap    62280..62379
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(62604..63290)
                     /locus_tag="PLG98_01335"
     CDS             complement(62604..63290)
                     /locus_tag="PLG98_01335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE31922.1"
                     /translation="MASLKIRQTTIKAVIFDFDGTLAKLTIDFSKMRSDIEALVASYR
                     IIPQELKTTFVLETIDAAHDLIRRQSAQQADAFFSRANRMIEDIETQGAQNGHLFGWT
                     KDLLESLQAAKISCGIITRNCVRAVNMVFPDILTYCPVVVCRDDVRRVKPHPEHIRLA
                     LDRLGALPDNTLMVGDHPIDITTGRNARTLTCGVLTGRCPKENFVAAGADLVLGNAAE
                     ILSILKIADG"
     gene            complement(63295..64131)
                     /locus_tag="PLG98_01340"
     CDS             complement(63295..64131)
                     /locus_tag="PLG98_01340"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOE31923.1"
                     /translation="MNLFQNKVALSRLIVEKLAALPVDFKEKDAEIDASRKQGSAHLK
                     AAVVLPLYFKEFNGRSEYVFQLIKRSESISQGGDISCPGGMLQPRQDNMLSSFLKRQM
                     LPALGFRITGHIQHADADTDRLIRLFLTTALREAWEEIGLNPLNAKLLGALPSYSLTF
                     LSRTIFPLVCRIRRPYSYRLSSEVEKVLEIPLSFFFESENYATLDVHMTVGESNEPFC
                     YQTPCLVIPDAGGNNDILWGATFNIIRNFLQVISGGTVPEATSARMMTKTLTNRYISP
                     RG"
     gene            complement(64150..65133)
                     /locus_tag="PLG98_01345"
     CDS             complement(64150..65133)
                     /locus_tag="PLG98_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE31924.1"
                     /translation="MDEKTNITDDPFEPALFLRNPHVQSIMASSRLRLHYHWRNELAR
                     NTEEVILKTSTGSRLVSYLTRHPEPKGMFVFLHGWEGSSSSIYILDSADYFYRRGYMI
                     GRLNLRDHGDSHHLNEDLFHAARLKETFDGVDYLASFSRIKPVYLVGFSLGGNFAMRI
                     ALRHGMKPINHLEHVFAISPPMNPYKATMAIDNGRAFYRYYFLKKWKRSLMKKQKLFP
                     DRYDFSDLMDVESCMELTKMLIPYFPEIQSYQHYFKMYTLKYNAFKYLSLPLTVLIAE
                     DDPIVSYQDFLNMDANHNLKILKQRYGGHCGFLDLFPASCWYQKAIAAIVS"
BASE COUNT        17077 a        15721 c        17001 g        15470 t
ORIGIN      
        1 aatcgactgg caagggacca ggaggtccgg caggccatgg ctgatttttt ccccaggatc
       61 gagaccacgt tgtcggatgg ctatattgac caacatcatc cttatcttct ggatgatgaa
      121 tttcatccga gatcagcgac cgtcagactc aaccagaata tattccgggg aggtgctact
      181 ttcgcggaaa caaaacgtca aaaatccaga gtgatttctg cggcctatca attgcagggg
      241 gtttcggaga atacggcatt gctggcgtgc agagtttact taaattatct gcgggccatt
      301 gagcaggata acctggcgaa agaaaatctt ctgattcatg agcgatttta cgatcaaatc
      361 aagttaagaa gtcaggcggg tgtcgaccgc agagttgatt tggatcaaat taccgcccgt
      421 ttggccctgg ccaaatccaa tctgattgtt acccaggcaa acatcgagaa tgccaaaacg
      481 gattatcagg ccgtgatggg atacctgccg gttaatttgg taaaaccgga accggcctac
      541 gctgaaatac cggccacgct gcaggatgct gaaagaattg ctctggccag tcatccgacg
      601 ttaaaatcgg ccaaagcgga tattgaagcc agaaattatc aacacaaaac ggctcgggcg
      661 cttgcctatc ccaccgtcga tgtcggtgcg ggatatacct ggggagagga tttgaaccgg
      721 ccggccgctt cttatgacta tgaagattat ttacagctca atgcgacgct ttctttcaat
      781 atttttaacg gattcattaa tcaggcaagg atcaaagaaa catcgtatct gattaacgaa
      841 gcggaagaaa tcgccaagaa aactgaactt cagaccattc agtccgtcag gctgtcgtat
      901 gagtcgtatt tggccgatca gcggcgcgtc agggaactgg aacagtatgt cgactctacg
      961 gataaagcgg cggaggcttt tatttcccaa tggtccatcg gaagacgtag tctttttgat
     1021 gttctcgatt cgtccgctga aaaaatttcg gcaaaaagcg atttaatcaa tgctcaatat
     1081 gataaaatgt acagcgcgta cagaattctg agcggtttgg gcaatctggt tcacaccctg
     1141 gggctgcagt ggccggaaga aagtcgggtg gataccggtg cgggcattgt tgatatgagc
     1201 tttcttgaaa aataaccgga taatgtcaaa caatgcgctt gattgagaac atcaggaata
     1261 ggcggatatt cttttaatga tcttttgctg acgaaaagat tccgcccatg atccgtgcag
     1321 aaatgaaatt gaatgtcgtg ctcaagctga tatctgaacc cgataaaagg tcgcgttcag
     1381 aggtgttgat ctttgcatga ccggatccca tggcttatga aatactggaa aattacaggc
     1441 gtgatcagcg cggcactttg tcttcttatt acctatcagg catgggcaga cagggaatgg
     1501 gtctacctgg aatccaattc ttcaagagaa tcattttatg acaaaaactc cattgtgaaa
     1561 gtggatgaaa atgtctttcg tttatggatg aagcaggtct taagcgaaaa cggaaaaata
     1621 aaaaccatat cgaaattccg caacacgaac aaaccggata acagccttga tttgatcagc
     1681 tatattttaa ggcagatgga cgtcgactgc cggaataatc aatataaaga cagcgccctt
     1741 atcttttacg atgaaaaaag caacgttctt tattcttccc cgaagggtgc gatagggaaa
     1801 tgggatgata tcgttgaagg gtcgtttgcg taccagttga aaaacgtaat ttgcaaagga
     1861 ccgaatcttg ctgagaaacc ctctgcgatt ttatccgccg accatgagga aaagccggct
     1921 gaagcggaca ctcctccgca aagtttgccc gccggtactt ccgatgaaga tattcgtcag
     1981 gtggtcagta aatggctggc ggcctgggag tcgggagaca tcgcaaccta tcgcagttgc
     2041 tatgatgaag attttaaatc gaaaaatatg acgctggaaa agtggattgc atataaaacc
     2101 ggcgtgcgtc aaaaaagcac gggtataaag atcggcattg acgatttaaa aattatcgcg
     2161 gatggagatc acgcggaggc ggtttttatc cagacataca gctcttccct attgaacgat
     2221 aagggtaaaa aaacgctgaa gcttaaaaaa gtcggcaatg aatggaaaat atatcaagag
     2281 atcatgtgaa gccgcatgca cgcagccatg agcctgcacc gaaggatcat ggccgtcccg
     2341 ttttttcctg tattgtcgat ttccgtgttc gccccgaagt cctccgttgt gcgatccgtt
     2401 cgcgttataa atgtcctatg tcggggatcc cataatttat tcaggaaatt gtgaattaca
     2461 caaacttttt tcctctgctt actgcttgat ttttccccgt gctctgatat aaagccccaa
     2521 tcacaatgca tttgctgccc tgatcaggct ctaatctgct attccattag tcaaggcggc
     2581 aggaacagga aggaagtata aattaaatca tggattatga ggggtttatc atcaggccgc
     2641 caagcgaagc ttacagtttg cttttacagg tgaccacggg atgttcacat aataaatgta
     2701 cgttttgcgg cacttaccgg caaaagaaat ttaaaattaa accgattgaa cgcattaaaa
     2761 aagatcttga cgaggccaga caatatgagg acgtcgacag agtgttttta tgcgacggcg
     2821 atgcgctgat catcccgcag ccgaaactta aagaaatact tgagctcatt aacgaaaacc
     2881 tgtccgatat tgagaggatc ggaacctatg ccaatgcgaa aagcattttg agaaaaagcg
     2941 ttgaagaact caaagaatta aaatcgctgg ggctgaaaat tgtttatctg ggtgttgaaa
     3001 ccggcaatgc ggaactattg aaaaaaattc ataaaggtgt aacgtgtgag cagatgatcc
     3061 aggccggccg tcgcattaaa gaagcgggca ttattctttc tgtaacagtg cttttggggt
     3121 tggggggcag ggaaaaaagt attgaccatg ctctggatac ggcaaaaatt ctgacggata
     3181 tggatccgga ctatgtcggc gcgctgacgt tgatgctgat tcccgaaaca gaaatgtatg
     3241 aggattatct tgcaggtcgt tttgttcttc ccgatcaatt cggttttatc cgggaactat
     3301 acctgatgat cgaaaaatca tcttttacgg actgctattt cacatccaac catgcgtcga
     3361 attatctgcc ggtcaaagcg cacctgccgc gtgaaaagga aaaaaatctg aaaatgatcg
     3421 gctctgtgat tcacgaaaaa gaccttagcc ggctcagacc ggaatatttg cggggcctat
     3481 aactcaggga gaagatattt gaacatcaaa aaaaacattt ccaaactttt tttctggcgc
     3541 gaacaaaaat atatcaaaaa caaaatcaat gccatgttgt ccgtcgccca gaaaagcggt
     3601 ttggtcgatg cgtcgtcccg ggagatgatc gagaatatcc ttgagttttc ccacacgctg
     3661 gttcgcgaaa tcatgattcc gcgcacggat attgtcgcgg ttagcacgga ggcggcaata
     3721 gatgacatca tccatgaggt tattgcctca agacatacgc ggatccccgt gtaccgggat
     3781 accattgata atatcatcgg catcctcaac gtcaaggaca tcctcatgtt ctggtccaag
     3841 ccgatgacca aggaagattt gattgcctgt ttgagcacgc ctcattatat tccggaaaca
     3901 aaaaacaccc acctcctgtt ttatgaattg aaggaaaata aaaagcatat ggctatcgtc
     3961 atcgacgaat acggcgggac ggcgggtctg gttaccttgg aagacatgct ggaggaaatt
     4021 gtcggagaaa tccgcgacga acatgatggc aatgtcaccg atgaccgcat cattcagtcg
     4081 ccggacggat ctctcatatt tgacggccgc atggagattg aaaaaataga agaaaacctg
     4141 aacatcgatc tgaaaaaagg cagatatgaa acattgggcg gtttgatcct tgatgcgata
     4201 aagcgcattc cggtttccgg agaaaaattc cagatcgaag gactggacgt taccatagac
     4261 aaggccgatg aaagaagtat caaaaaggtc aagataaaaa agattgaaac cgacaacgac
     4321 gatgaaaaat aattttcccg aagtctcttt ggcagacatt tcccggaatc ttttccggaa
     4381 gaatatcttt tttgccgcgt tgagcggtat cctgctgttt ttgtcgtttc cgaagtacgg
     4441 atccggtgta ttggcctggg tggccctggt gccgcttttt tacgcgctga aaaacgctgc
     4501 gaccatccgt catggactgc ttctgggttt tattaccggg atcatcagtt atgtggggat
     4561 catttactgg attgcccacg ttattgtaaa ctacgggcat cttccgcttc atatcggaat
     4621 tgcgctgatg ctgcttctgg ccgcgtatct gagtctttac ctgtcgtttt ttgccggatg
     4681 tcttgtgtcc tttcgtgaga aagtaccgtt ttacatcatc gggccggctt tatgggtttc
     4741 tctggaattt tgcaaatcct ttattctgac aggttttccg tgggagaatt tggggtattc
     4801 gcagtacgga aacacctatc tgattcaggt tgctgatatt ttcggtgttc cgggtttgtc
     4861 ctttctcatt gttttggtaa atgccgttct cttggatgtg atccacagga gaacaaaaaa
     4921 agaatatgtt gccgccggca tggtttttgt tctgattgcc ggaatctatg tgtacggatt
     4981 tttccgtctg agagagattg atgcgacggt gacaaacgct ccgggcattg acgtgtcgct
     5041 gatacagggc aatattgacc aggccgtcaa atgggatcag gattacaagc aggagacatt
     5101 ggatatctat gaacggctat ctttaaaaaa caagcccggc cggggcagtc tcataatctg
     5161 gccggaaacg gcggttccgt ttaaatttca aaacatcaac cgttttcgcc gtcagatcat
     5221 tgacctgtct ttaaaaactc aaagctggct tctgttcggc tcggtgagtt atgagcccgg
     5281 agcatacggc aatgattttt ttaacagcgc ctatctgctg tctccctccg gtgacgtcag
     5341 aggacgctat gataaagtcc atctcgtacc gtatggcgaa tatgttccgt tcagggatct
     5401 catgcccttt gtcaaagggc tgacgtcagg tatcggggat ttcagttccg gggcgggatt
     5461 tgatcccctg tccctgaatg ataaaaaaat aggggttctg atctgttatg agggcatttt
     5521 accgtttgcc tcgcgagcct ataaaaataa agccgccgaa ctgctggtca atattaccaa
     5581 tgacgcctgg ttcggagcaa cgtcggcgcc ctttcagcat ttctccatgg cggttttcag
     5641 agcggtggaa atgcgcctgt acctggtcag agcggccaat accggaattt ccggaattat
     5701 tgatcctgcc ggaagaatta ttgcaaaaac gggtatcttt caggaagagg ccttgaaggg
     5761 gcatgcaaaa tttgtcacaa taccgacggt gtatgaacga gtcggggata ttttcatcat
     5821 cgggtgtttt atgatggtga gcatctgttt tattttcagt ttaagacgaa ggagtcaaaa
     5881 atgaccacgg aaaacattct ggaaacgatc agcgatctga aaagaagaat tcagcatatc
     5941 ggggagtgtc tttgacgttc cggaattgaa aatgaaaata aaagatctgg aggagttatc
     6001 cttaaaaaag gatttctgga atgatcagga ttatgcccgg tccgttctcc agaagaagac
     6061 aaatctgtcc gaaaaagtgg aaagatggga cagattcagc aaagacatca gcgacattga
     6121 aaaccttgcg gaaatggccg tcagagagaa agacgagcat gtcctgcagg atatcgatga
     6181 agagctggaa cagttgaatg cggccgttgc ccatgaagaa ctgaagatga tgctcaacgg
     6241 cgagcaggat gctatgaatg ccatcgtatc cattcatgcc ggcgcagggg gaacggaggc
     6301 gcaggactgg acggaaatgc tgttgcggat gtatctgcgt tgggcggaga agaaaaaatt
     6361 tgcggccagg atcatcgatt atctgccggg ggatgaggcc ggtgttaaaa gcgtgtcgtt
     6421 tacgcttgaa ggagagtatg cgtacggata tgccaaggcg gaaatcggca ttcatcgtct
     6481 ggtccgtatt tccccctttg atgccggtgc gcgtcgtcat acatcgtttg cctccgtgtt
     6541 tgtatatccc gaagtcgatg acgatatcaa aattgaaatc aatgacgatg atttgcgcat
     6601 cgatacgttt cgtgccggag gcaagggggg gcagcatgtc aataaaaccg attcggcggt
     6661 aagaattaca cacgtgccga cggggattgt tgtacagtgc caaaacgaac gttctcaaca
     6721 tcagaacaaa gcgatggcca tgaaattttt gaagtcgcgg ctctatgaga gggaactaca
     6781 gttaaaaaat cagaaaatcg atgaggaaaa caagcagaaa aaagacatcg cgtggggcag
     6841 ccagatacgg tcctatgttc tgcatcccta caaactggtc aaggatcacc ggaccaatgt
     6901 cgaatgcggc aacgccggta aagtgctgga cggagacatt gatgaattca ttcaagccta
     6961 tctgatgatg acgacaacga cctcgtaatc ggatgggcca gtgcacggca acttctcctt
     7021 tgatcgtcgc gtttttgtcc aagggaaatt taatttttgg aaaaatgaaa aattatgcta
     7081 taaagatgaa ccatacggta cggccatgat ggaaggatat gcagaagcgt gtcgtattta
     7141 tgaataagga gttctgaatg accggattga caagaaaccg tctgataggc ctgattgctc
     7201 tcatctgtct tttcatcgtc cctttaatcg gatatgctga aaattctatt gacaaaaaaa
     7261 gtccggagtc ctccggcgac atcacgaaac aaaaaataga ccagaaaata aagctcgccg
     7321 cgttgaataa cgacgacgaa agcagcctgt ttgattttcc gcaggaaaac gatcccttgg
     7381 cggatgttca ggaggatgat gaaaaacagg acatgatgga aaatgcactg gagttattgg
     7441 gtgaggccga caaactttgg aaaaacggag atatcgaaga aaccctggat acactggatg
     7501 aggcttattc gttaattctt aatgccaacg gcgattcggc aatcgctcag gaaaaagacg
     7561 atctgcgtct tttaatttcc cagcgcattc tcgctgttta ttcctccaaa cgggatgtgc
     7621 tccaggggaa aaacggcgag attcccttgt taatgaacgg cgatgtgcaa aaggagatca
     7681 cttcttttca gaccgtcgag cgcgacagtt ttcttgccgc ctatgagcgg tccggcatgt
     7741 accgttctga aattgtgaaa gagcttaaac ggtccgggat tcctgaagag ttcttctggc
     7801 tgcctcttgt tgaaagcctc tttaaaatca atgcttattc ttcggcccgc gcactcgggt
     7861 tatggcaatt cattccttcg accgggtata agttcggact gaatcgcgat gaatgggttg
     7921 atgaacggat ggatgtgcaa aaatccacca aggcggccat cgcttatttg aaagaactgc
     7981 atggtatgtt cggcgactgg cttacggcgc tggctgcgta taattgcggg gagggcaggg
     8041 tcctgagggt gatttcacga cagcggctca attacctgga cagcttctgg gatttgtaca
     8101 ggcaattgcc gaatgaaaca gcgcgttacg ttccccgatt cctggccacg ctgcatatcg
     8161 tcaacaatcc gcaaaaatac ggatttgatt tgagccatac cgccaggccg attgaatttg
     8221 aaacggtccg ggtaaacagg atcatgaagt ttaacgatat tgccgaaaga atcgacacga
     8281 cggaagatat cttgtgcctg ctaaaccccg aattgaggca taagatgacg ccggatcggg
     8341 aatatgattt aaaagttccg ggaggcatgc tggataaatt cagttttgtg tatcatgaaa
     8401 tcccgtccag tgagaagccg agcatcgcgt ccgtccagac gtatgtcaag gcatccgacc
     8461 gaacacgcag gacctacatc aagcatcgtg tcaagagagg cgagaccctt tactccattt
     8521 cgcgaaagta taaagtttcg atttccagca ttcgatcctt gaataaatta agtccgaaaa
     8581 agaaactgat tcaggggaga agactgctca ttcctgttgc gtcggccaaa cggcatgcaa
     8641 agggattgac cgtaaaaaaa aaattcaaaa agaaataacg tccataggtc gttataaagt
     8701 taaaaaaggt gaaaaccttt ctacgatagc ctgccggttt tcggtgtccg tatcggaact
     8761 caaagcgatc aataacctgt cgtcgggaca aatttacgca ggtcagcgct tgaagattcc
     8821 tcaaaaggaa gacggaaagg attccggcaa tgaagattcc gaaaaaaatc tgagaacatc
     8881 cgctcaaaaa tcaaaaatcg ataaaaaaat attgtcggca aaggatatca atcagctggg
     8941 gccggacaaa tatattgtca cgaaaaacga taatctgcac accatcgcca aaaaaaataa
     9001 taccaatgcg gccagaatca tggagttgaa tggcctgtcc gttgatgcaa agattgttcc
     9061 gggtcaaatt ctggttgttc aataatcatc gcggaacgta tgaatatatc atgacttatt
     9121 cggaaccgta tttattttct tgacaaaaaa agacgaatac tgataatgaa accctaaatt
     9181 cgcttggatc cgaaaggact gagacggaag gaaaatgcat cgaaaatttt aaagcctttc
     9241 gtcttgcgat gaaaggcttt ttttattcga agatgaacgt cgtaaagtca gttcagaaaa
     9301 tgcagtcttt ctctgaaaac ctgcgtttgg agggaaagaa aattgcatgt gttcccacca
     9361 tgggctactt tcatgaaggt catcttgcat tgatgagaga agcccgcaag caagcggact
     9421 gtgttgttgt cagtatttat gtgaatccga cgcagttcgg gcctcaggaa gatttctcca
     9481 aatatccccg taattttgat cgagatatga agatggcgca aaacgtaggc gtagatgtca
     9541 tcttttaccc gtcgaaccgg gcaatgtatc ctccgcacta tcagacctat gtggcggttg
     9601 aaaaagtcac gcagaatctg tgcggagcat cccggccggg ccattttcgc ggtgtgacca
     9661 ccgtatgcaa taagttgttc aatattatca agccgcatgt tgctgtcttc ggcaaaaagg
     9721 attttcaaca atttgtcgtc gtccagcgca tggtgcgtga tctgaacatg gatctggaga
     9781 tcatcgggta tcccacggtc agggaagcgg acggactggc catgagctcg cgcaacaaat
     9841 atctttccgc cgaacaaaga acctctgcat tggttttatt caagtctctc caactggctc
     9901 aaaaactgta cgcgaaaaaa gagacatcgc cccgtatcat tatcgatgcc gtgaaaaaaa
     9961 tgattcaaaa aacacctctt acacaaatcg attacgtgaa aatctgtgac acgacgcgcc
    10021 tgcaagatat cgcaaaaatc acagatcaga ccgttattgc tctggctgtc agaatcggtt
    10081 cgactcgact gattgacaat catgttttcg gtgaagcgct gaaaatatca tattgaattt
    10141 tttaaggaga cacttaaacc atgcagagat ttatgctgaa atcaaaaatt caccgggcaa
    10201 cggtcacgga tgccgatctg cattacgaag gcagcatttc cattgatgaa aaactcttgg
    10261 aagcagcatc cattttgcct tatgagaaag ttgcgatata tgacgtggac aacggggaga
    10321 gattcaccac gtatgcaatc aagggaaaac gaaattccgg aatcatctgc ctcaacggcg
    10381 ctgcggcccg taaagtggct aaaggggacc tgatcatcat tgccagttat gtggtggtcg
    10441 aggaagcgga aacgaaaaac tggaaaccga cctgtgtctt tgttgacggt aaaaacaaga
    10501 tcaagaagaa acattgaccg tatgaaggtg tctgaccgga tcaatgtgcc catggatatc
    10561 cgccggatat tttttgtctc gaaaactgaa gttcaatccc cataaaaata ttagactaca
    10621 ttcatgacct gtgttattga tgtgtatatt tgacgcaaca tcatgcagac taacgtgctg
    10681 cttaaaaaaa tgaaatcaaa cttggaggaa acatggcttt tttggaaatc gatcctgctt
    10741 taaaatataa agtggcggac atagcactgg cggactgggg aagaaaggaa atcaaactgg
    10801 cggaaaatga gatgcccggt ctgatggcgt tgcgaaaaaa atacggacag aagaaaccgc
    10861 tcaagggcat gaaaatcatg ggcagtctcc acatgaccat tcaaaccgcc atgctcattg
    10921 aaaccctcaa gacattgggt gcggaaatcc gctgggcgtc ctgcaatatc ttttcgacgc
    10981 aggatcacgc cgcggcggca atcgccaaaa aaggatcggc ggccgttttt gcctggaaag
    11041 gcgaatccct tgaagagtac tggtggtgta ccgagcaggc tctgacctgg ccggacggat
    11101 cgggcccgca tctgattgtc gacgacggcg gcgatgcgac cctctttgtt caccagggtg
    11161 tgaaagttga acgagatccc gcgttgttga aacaaaagat cgacaataaa gaatttaaaa
    11221 tcatcttgga ccgtctggaa aaatcattcc ggcgcaatcc gaaaaaatgg cagaagatcg
    11281 cctcaaccat caaaggcgta tccgaagaaa cgacaacggg cgttcaccgc ctttatcaga
    11341 tgcaggcggc gggagaactt ttattcccgg ccatcaacgt caatgattcg gtgaccaagt
    11401 ccaaattcga caacctttac ggttgccgcg aatcgctggc cgacgggatt aaacgaggca
    11461 cagacatcat gatatccgga aagattgttg tcgtctgcgg ttatggcgat gtcggcaagg
    11521 ggtgtgcgca gtccatgaga ggattcggcg cccgggtcat cattacggaa atcgatccga
    11581 tctgcgcctt gcaggcggcg atggaaggat acgaggtccg gaccatggag gaagtggcgc
    11641 cgatcggcga catttttgtt tctgcaaccg gatgctgcga tgtcattaca ggccggcaca
    11701 tggaaatgat gaaaaacgag gcggtggtat gcaacatcgg ccatttcgac agcgaagtgg
    11761 acatgcatta tctggaaaca agcaaagtct gcagaaaaga aaccatcaag ccgcaggtgg
    11821 acaagtggac gctgaaatcg ggacgctcca tcatcgtact ggccgaaggt cgtctggtca
    11881 atctcggctg tgcaaccggt caccccagtt ttgtgatgag caacagcttt accaatcaat
    11941 gcctggccca gattgagctt gccactaaaa aacataaaat aggcgtatat acgctaccca
    12001 aggtgctgga tgaggaggtc gcccgtctgc atctggaacg actggatgcc cgattatcca
    12061 aactgacaaa aaagcaggcc gattatctgg gcattaccgt ggacggccct tttaaaccgg
    12121 atcattacag atattaaatt ccgggatgct ttaagaataa acgcgttgcg ctcaggtatt
    12181 gactttgtat aaaagaaatg cggcggcagc accgaaaaga atgtttgccg cccaggcggc
    12241 caacacaaca ggcagcattt ctgatttgcc cagagacatg cagaaggcat ggacgatcca
    12301 gtaggaaaaa ccgataaaaa tgcctatgcc gatgctctgc atgacgccgc cgcttcgttc
    12361 ggagcgcaga gcaaagggta cggcaatcag aaccataatc aaaatcacaa aaggaaaggc
    12421 cgtcttgccg tgcagatcga cgttgtattt gcgggcatcg aaaccttccg attgtatctt
    12481 ttgcacatat cggcgcagtt caaaataccc cattttttca acatccttct gcattacttt
    12541 gaaatcttcc ggtttttccg gaagcgggat gattttttct tttgagcgtt ccagaaccgg
    12601 ggcacggtta tcatcgaagc gcgtcgtaag cagatcatga aaaacccagc ggccgttttt
    12661 ccattcggcg cggcgggcgt caatgcggct gatcagggta aagtccttgt ttaaataagt
    12721 gatcatgacg ccgtgcagga tgttttttcc gatgtcaaac attttgaaat tatagatggc
    12781 attgtcgctg cggtaccaga tttcattctg tttaaagaag ccgggcgttt tttgtttttt
    12841 cacctcgatg tttttgatgt aggtcgtttt ttgaacggcg gccggtgtaa tcagttccgt
    12901 aaaataaaag taaaacaccg cggtgagaga ggcgaacatg acgatgggca gagccattct
    12961 gaagatattg atcccgttgg ctttcatcgc ggtgatttcg ctgaatttcg tcagggagct
    13021 gaaggtcatc aatgtggcca gcaggatcga gggaggcaac acataggata taatcatggg
    13081 aacggagaag ataaagtacg acgccatttg tgccgccgtg gcctgatggc tcagaaacat
    13141 tctgatgcgt tcgaaaaaat cgacgatcaa aaaaagcgca atgaacgcca gaaacgttat
    13201 cgtgaagaac tttaaaaatt ctttcagaat atatttatcc agaagtttca cgtcgacgtc
    13261 ctcggatgag tctccagtgt gccgggagca ataaaaatat gtttaaaaaa gataaatctc
    13321 tttgctggtt atgtaaaaca tgaaaatgcc gatgaaagca aatgaaatat tgggaatcca
    13381 gacgccaacg gccacgggga tgtagcctgt ttcagccagc gcttcgccgc cgatgcgcag
    13441 cagataatac aagcaaaccg tgatcaggcc gacggcaaat cctctggcct tgacggagcg
    13501 ctggcttcgc atgccgagcg gcagcgccag cagaccgaaa aagatgcacg ccagcggtat
    13561 ggaaaatttc ttgtgggctt cgatggacaa ttcccgaaca tctttttcgc tcatcccggg
    13621 ttttttcatg ttttgcagta attcggtcat ggtcatgtct ttggcggctt tcgacgcttt
    13681 atcgaattca gccagggcgg catacaaatc cagattgatt tcatagctct gaaaatcaat
    13741 tttacggtaa ttttttaaat cggaactgac cgtgtggata gatccgtttt ccatccgaag
    13801 cttcaccctc atggtgtcgg ggtcggaaac caggaaggcc cggggtgcga taatggtgtt
    13861 ctgttcgccg atggtgcgtt tgtcggaaac gatgacctct tccaaagcgt gattcccctc
    13921 ggaaatttta ttggtataaa taataaagcc cttaaagctg tcattaaaaa ctttttcctt
    13981 gatgccgaca ctggcatttt gctgtgcgat atcgaaaagc agccgcttgg tcgcgaaatt
    14041 gctctgtggt gcgacgaaat aactgaccag cagcgtgaaa acaaacgcga gcagggaagc
    14101 gaccgcaacc ggataatata ttctggccat actgacgccg gatgtcttca gcgccgtgat
    14161 ttcattgtcg gaggataatc ggccgatcgc gatgaggatg gagatcagaa gcgcaatcgg
    14221 aatggtaaaa agcatgaaat agggcagcat aaaaatgaaa atttttgcga tggaaaaaat
    14281 gctgacgcct ttattgatga acaagtccat gatttgaaga attttcccca tcagtaaaac
    14341 gaacgtcagg acgcaaagaa tcagaataaa agggaacgcg atttccttaa atatatagcg
    14401 gtcgatgatt ttgcgcatgg cctgatcaac tcttttttta atggatttcc agacataaaa
    14461 agagcgccgt cgcgatcaaa aggatcccgg gtcatgccct gttttttgtc cgggttgaaa
    14521 ttaacacgca atgatgaatt ttctcaagta aaacatgcag agattctgaa accatgtttg
    14581 catgattcag gcggcggacg ttcgtgaaat gaccgacgga tttcgaaaaa aaacgattgg
    14641 ccttccattc attgattgac agtttcaatc atttttttta tggtgatgcc aaatcttacc
    14701 cgttgtgaaa gggatcggtc tatgaaggtt gatacaatgc gccggataga tttttatgca
    14761 ggcgtcccgc tgtgtttttt cgggtccctg ttttataaag cggtgaaatg gctttccccg
    14821 cggacaaaga aagatccgaa aaatgtcctg tttatagaac tgtcggaaat gggcagcgcc
    14881 attcttgtgg atccggcaat gagaaaactg cgcgacacaa ccggcgccca gttgcatttt
    14941 gccatcttta aaaaaaacaa gcccagcctc gatctactgc aaaccgtttc gccggacaat
    15001 atttacacca tccgggagga ccgtctttcg tcttttctga tggacacgtt taaattcctg
    15061 cattggacga gaaagagaaa aatcgacacg gtcatcgatt tggaactgtt ttcccgtttt
    15121 accgcactgc tgaccggttt cagcggtgct tcaaacaagg caggttttca tgcctatttc
    15181 aatgaaggtc tttatcgcgg cgattttctg tcgcataaag tcgcctataa cccgcacatg
    15241 catatcgcga aaaatttcat cgctttagtc aatgccctgc tttcgccgaa agaagaaatc
    15301 ccctattcca aaaccaggat tcaggatgca gaaatcagca tggccaaagt gcacatcacg
    15361 gatacccaga aaaagacggt cctggacatc attcgcggat gctttgatgc tttcgatcct
    15421 tcccggcata aaattgttct aatcaatccc aatgccagtg aaatgcttgt tcagcggcgc
    15481 tggccgcagg cctattacgg acacctgata aaaatgattc tgggacaatg ttcccatgcg
    15541 gttgttctga ttaccggcga tccgcgtgag cgggaggagg cagagcgcct caaggaacag
    15601 gttcaggatg agcgatgtgt taacttcgcc ggcaaagtat cctttgccca gcttccccaa
    15661 ctatacagcg tctcggaatt tatggtgacc aacgattccg gaccgccgca ttttgccgcc
    15721 attaccgaga tgcccaccta cgtcatcttt gggccggaaa cgcctgcact ttacgggtcc
    15781 ctgggccgga ccacgccgat ttatgccgga ctggcctgct cgccgtgtgt cagtgcatcg
    15841 aaccatcgaa agacggcctg cacagacaat gtctgtctga aggtgattac ctcatcgcag
    15901 gtttttgaaa cgatcaggcc tgtgctcgac agaatcaatg ctgagactct tctgtaaaga
    15961 aagcgcggtc agcttttttt aaacagcgct tccagtttgt ctccgggggt gttttgagag
    16021 ggtgcatcgg atagcgtttc cgtttgccgg aatctgaaga aatcgcagaa attggagcgg
    16081 gctttatcca ggacgcgttc cgcttgcggt tcgcggcagt cattatacgc gccggcatcg
    16141 taaaaagagc agttcaggca gcaatgcaaa tccgcgctgc agacggggca ctgggattgt
    16201 cgtccggtat aaacattatc cgaaatttca ttgaggcatt tgtgacatcg tcgcatcatg
    16261 tcaaaaaaat acatgttgtg ttgaattttt gtcaagccaa tttgaaagca acaatgcatt
    16321 tccgagcgcg acggaaagca gggcttcttg atttcattat gttttaagcc aatttctggc
    16381 caggtcaagg ttgctgaaat atctcgcttc caaatgggtg ttcagggtgg ctgtttttaa
    16441 aaaagtttcc aatctgtcat tgccgggaac atccaggata gccgtctttc ccgttgcctt
    16501 atcgggacgt ctgatataat aaagaacatc gtggtatccc gagccttcat taagggttcg
    16561 cacgtcgatc agcaaccgac tgcctttttc attcctcagg gaatcaatct ccttttgcaa
    16621 ccgcgaaaca ttggttcttg ttaccgtgcc tgaaagaata atttcaatga ttccgtcgtt
    16681 tatcgatgct gacaggcggt attccttatc gtttgtcact ttttcccctc acgatcgcga
    16741 tacgctattc tcgtccgccg gacgatgatg aattttgtaa aagcggtgta ttcatctctc
    16801 aataatgaaa aatataattt tgcatatact cagattgtaa aaaaatatca atccctcgaa
    16861 agtgggattt tgacgtgtct gaattcgcca tgcaacgagg aggcccgggg cgggagaaaa
    16921 ctcttcagag ggcttgatca gcgtattttc aatccggcgc aaccggattt ttcttgtcga
    16981 ggatgcgtgc caccagcata cccgaaacat tgtcgccgac agcattaagc attgttgccg
    17041 gtgcgtcaac aagcgttcca atcatcgtaa tgatcggaaa agcttccatc gggaaaccat
    17101 aaaaggaaat aatcagcagt tcgccgatgg taccgccgcc gggaatgccg ctgaccacca
    17161 cgccggtcag caaagcaatc ccaaaagccg ttaaaagggt ttcggcacct gaaaaaggca
    17221 tattgaagag gccgaaaaga aacgcaatct tcaaaacggc ggccaggcac gagccttcca
    17281 tatgcatggt tgcgccgacg gggataacga tctcgcgaat atcccgggga acgccatttt
    17341 tatcggcggc ttccagattg gcgggaatgg cggccatgga actgccggtg gccagggcaa
    17401 tcagagatgg cggaatgatg tttttccaga agcgacgcat gccatcgata ccggcggcga
    17461 taaaagcgta caggctgaaa gcgatgaaaa aatacaaaaa agccgtgggg taataaacca
    17521 tcatggcccg tgcgtaagag tcaagcagtt gcggcccgaa aacgccgacc aaataagcga
    17581 aataggcaca gaggccgacg ggagcataat acatgataaa ggaaatcact ttcatcatca
    17641 cggcgcttcc cgaattcaga aaattcgcaa acggttttcc gcgctcaccc gtcacagagg
    17701 cggccaatcc ggttaatact gaaaaaacaa tcagggccag catgttcttt ttagaaagaa
    17761 gctctgtgaa atcgccgacg gtgaaagcct tgacgatctg gtcggaagct ttgaggtgtt
    17821 cgacatcaac accgttaccc agaccgatgt tcaagcctgc ggccggcggg aaaaagatca
    17881 cggcggcaat catgaccgcc gacgcgatca ggccggttgc gataaaaaca aaaatcatcg
    17941 cagacatgat ttttcccagc cgtcggatat tttgcatacc ggcgacggcg gaggctatgg
    18001 aaaagaagac cagcggaacg attgcggtaa agatcagatt caggaagata tcgcccaggg
    18061 gtttgaggcg tacggcatcg gccttaaaaa caaagcccaa aacggcgccg atgacaatcg
    18121 aagcaattag taaaatggaa aaaccgtacg attttaaaaa agatggttgt gtcggattca
    18181 tgttttcact cgataatgaa atcaaaatat gtgtcggggt aaggttcgtt tttcagggtg
    18241 tagtgccacc attccagatc gtaattcttg aagccgtgct tttccatcag cgatttgagc
    18301 agcagccggt tgatcttttg ttgttctccg atttttgcgt ttgccgtatg cgacagttca
    18361 tgaaaacagt caaatccggt gcccatatcg atgctgttat ccgcaaagcg ttttcccgaa
    18421 ggcaggtagc attcggataa ggtttgtccc ggggtgtaat cgggctgcgg cggcgcggac
    18481 aacaaaacga tagtcagatc aacggtgctg ccgcggctgt ggctggaccg gtcggcaata
    18541 aacccgtccg taaacagatt ccgtttgtcc accgtaggat agaattcttt tctggttttc
    18601 gtgttttcga tgtcctttgc ccagcggacg aaatgatgca ccgcccgttg cgggcgataa
    18661 cagtcgtaga ttttcagggt cagcgaataa gcctcaagat ccttctggac cagcgccagc
    18721 gcatcggccg cttctttcgt cagataacat ttcggggcca gatagccgtc aacccgttcg
    18781 cccaggaagt tgtgcgcacc gtgataacgg atatcaagca gcgcgcctgg aatgactgtt
    18841 tgaatatcca caaaacggtc cggctttttg ttcaggtcgg agcaatgcgc cgcggacgca
    18901 aaataaaaaa aagaaaatga aactgttact gccagtaaaa acctgatttt catgttatcc
    18961 tgctcccgga ttattatttc aggcccatga tgatcagcgg cgctgccgcc ggatcaagcc
    19021 agtgaagaag cctgaggata ttttcttcgg atactgccac gcaacctgcg gtgggaacgc
    19081 tcttgtcctt ccaaacatgg acgaaaatag cgctgcctct tccctggacg accgggttgg
    19141 tgttatacgc gatcacgatt ccgtatttat aaaggtcgtc atcacgtttc attctttcat
    19201 acgatgtcga ttgcgtcacg gattgctttg tccagcggtt ataatctgcc gcttcggaat
    19261 cgtcaaccca gatatcatct tctttgattt ggcgatagag cattttcgtg tttgcagagt
    19321 cagcataacc aaacgccatg accaggggga aaatgccgga aggggttttt ccgtcgcctt
    19381 ctcttttttc ccctggcggc gcaaaaccgt ttcgtccaat gacggcgtcc atgggggggg
    19441 agaccgccga ccagtttcct tcacgttgct ccatgacata aacgattgtt cgggtgaaaa
    19501 tccagaaacc gcagcgttgc acaagaagca cctgggaaga accgtccggt tgatgaggcc
    19561 tggacattaa aaaatccgca gcatcaaatt ttgtgttgaa acaggaaaag gcgcatccgg
    19621 aaacggatga aaacgccata attactgaaa atataaggac aaattttaac ggataaaata
    19681 tcttcataaa atcattcaat attttggaaa tgacggccta gaatacggta aaactataaa
    19741 aaaatcaagt aataacagca gacgaagtta tgcttgactt taaatatttt tctgtgatag
    19801 caaagtgcat ctgaatggca cttgatcgat aatctcataa taaatttcgc aggaaagaag
    19861 cagtgtccga taattttttt acatttatga ttattcccaa ccggaagagc ggggtcaaaa
    19921 aaatatccgt tccgaaggtt gccatccgta atattctcat tggcgccatc gtcgttgttc
    19981 tgatcgctct gtttgtcatt tatgattatg ccagcatcaa gcgcgaccgg gcggaactgg
    20041 cccggttgag agcgcagacg aaagaacaat cccgtcagtt gcaggatatg ggcatgaaaa
    20101 tagatcagtt tgccgatcgc ctggaagcct tgaggcagta tgacaaacag atccgggttc
    20161 tggcccatga aaccaacctg agcaagaaaa tgcccctggg cgtgggcgga tcagaagaag
    20221 tcagaatgaa ggatttggtt gataaagacc atgaacaaat tatcgcgggc atgcgcagaa
    20281 acttcgaaaa gcttcatgaa gaagccaatg aaagagagag aagcttcacc gaactattgg
    20341 ggtatttggg ccaacagaaa gccgttctgg cttcaacccc ttcgctctgg ccggtgaagg
    20401 gatgggtgac gtccgaattt ggcatgagaa gatcgccctt ccgatcggga atggaatttc
    20461 ataaaggcct cgacatttcc acccggttcg gaaaagaagt ggtcgcgccg gcggacggtt
    20521 tggtgatcag cagcggttac ggaccgcaag acggcaatta cgtcaaaatc gatcacggcc
    20581 atgggtttac tacagcctat ttgcatttgt ccagaatgac ggtgaaacag ggaacgcggg
    20641 tcaaccgagg agatgttatc ggttatgtcg gcgatacggg acgcagtacc ggtccgcatt
    20701 tgcactatgc tgttttagtt aacaatgtcg aggtaaatcc gagaaaatat ctgagataat
    20761 gattgactga ttttacctga agttgtattg atacattcac tgattgaata gaccggttaa
    20821 ttggccggtt tttttttaaa aaaatatgga attttagggt attgattgaa tgctggacaa
    20881 tatattgaaa aaaatattcg gcacaaaaaa cgaccgggaa ttgaaacggt tgtcgttgct
    20941 tttaagagag atcaacaacc tggaaccggc catgacggct ttaaacgatg aggaattaaa
    21001 agccaaaacc ccttatttca gggaaaaact caaaaacgga ttttccctgg acgatattct
    21061 gacagaggcc tttgccacgg cgcgggaagc ctcgcgccgg acgctgggga tgcggccgtt
    21121 tgatgtgcag attatcggag gcatcgcttt acacgaaggc aaaattgcgg aaatgaaaac
    21181 cggtgaaggc aaaacgcttg ccgccaccat gccgttatac ctcaatgccc tcgaaggaaa
    21241 gggctgtcat gtggtgaccg ttaacgatta tctggcgcgc cgcgatgcgg aatggatggg
    21301 gccgatttat aaatttctgg gtctgaccgt cggcgtcatc attcacgggc tggatgaccc
    21361 ggaacgcaga gaagcctatc actgcgatat tacctacggg accaataatg aattcggttt
    21421 tgactatctg cgcgacaaca tgaaattcac gctggacgat tatgtgcaac gcgaattcaa
    21481 ttatgccatc gtcgacgaag tggacagtat tctgatcgac gaagcccgga ccccgctgat
    21541 tatttccggt ccgtccgagg aatcaacgga caaatattat aaaatcaacc agattatccc
    21601 ccggttaaaa aaggacaaag attactctat tgaggaaaag agccgtacgg tggttctgac
    21661 tgaagacggg gtcgcacatg tggaaaaatt actcaatgtg cagaatcttt acgagccgcg
    21721 caacatagaa atcgtgcatc atgtgaatca ggcgctgcgc gcccataccc tgtttaagcg
    21781 ggatgtggat tatctggtca aggacggtca ggtggtgatc gtcgacgaat ttaccggaag
    21841 agtcatgccc ggccgtcgct acagcgacgg attgcatcag gcgctggagg ccaaagaaaa
    21901 cgtaaaaatc gaacgggaaa atcagacgct ggcgtcgatc acctttcaga actatttccg
    21961 catgtataaa aaactggccg gcatgaccgg aaccgccgac acggaagcgg ccgagttcaa
    22021 gaaaatttac aacctcgacg ttctggtgat tcctaccaac atgccgatga ttcgcgagga
    22081 ccacaacgat ctgatctaca aaacggaacg ggaaaaaatc aacgcggtca ttggggaagt
    22141 taagacgttg aacaaggaaa ggcggccggt tttgatcgga acgatttcca tcgaaaaatc
    22201 cgagatgctg agcaaacatt tactgcgcgc cgggatcaaa catcatgtgc tgaacgctaa
    22261 aaatcacgag aaagaagccg agatcgttgc gcaggcgggc caggcgggca tggttaccat
    22321 atccaccaac atggccggtc gcgggacgga catcaaactg ggcgagggcg tggcggagtt
    22381 gggcggtctg catattctgg gcaccgaaag acacgaaagc cggcgcatcg ataatcagtt
    22441 gaggggccgc tccgggcgcc agggagacag aggttcgtcg cggttttacc tttcgctgga
    22501 ggatgattta ttgcgcatct tcggcgcaga gcgtatttcc ggaatcatgg acaaaatcgg
    22561 cattgaagag gatcagccga tcgaacacaa atacatctcc cgcgccatag aaaatgccca
    22621 gaaacgcgtg gaagggcaga atttcgatat tcggaaacat ctcctggatt acgacgacgt
    22681 gatgaaccgt cagcgaaagg ttatctatga acagcgcaag aaagtgctgc gcggcgacaa
    22741 cctctgggcg gacacggagg agatgatcga ggaaatcatc gacaatattt tgtccgagtt
    22801 tgtcgacgaa aagacaaaga aggaagacat aaatttcaag ggtctggacg atgccctgct
    22861 caaacaattc aacataagat ttgatctggc gaataccgat cagacgggtg acggtcaatc
    22921 agccattcgg gaatggatca ttcagggcgt gcagaaatat ctgcgggaca aggaaaaaga
    22981 attcggcccg gagctcatga actacctgat gaaagtcatc atgcttcagg ccatcgatgt
    23041 ccagtggaag gaacacctgc tggccatgga tcatttaaga gaaggaatcg gtttgcgcgg
    23101 atatgcccag aaggatccgg tccgggaata tcagcgcgaa gggtacgaca tgttcatgga
    23161 aatgatcgcc cggataaaaa tggacgttct ggaaaaactg tgtctgatcc ggattcagcg
    23221 agaggaagaa gtcgaggcca tccggcagaa acaaaaacag gattacgtga tgagcagagg
    23281 ggaggatacg ccggagaaaa aaacggtcaa gcatgaaggc ccgaaaaccg gaagaaacga
    23341 tccctgcccg tgcggaagcg gcaagaaata taaaaaatgc tgcggagcat ccgtttaacc
    23401 gagaggagct aatttcatga tcaagaacaa attaaaaatt agcgtaccgg gatttctggc
    23461 cggcggtatt tccgttggca ttaaaaaaaa ggatcagaag gatctgggcc tgatctactc
    23521 caccgttccc gcccgggcgg cggcgatgtt tacaaaaaac acctttaagg cggcgccgct
    23581 tttgatcaat gaagaccggg tgaaaagagg catcgcccag gcgatcatca ccaacagcgg
    23641 ttgcgccaac gccgcgacgg gcaaagaggg aatccgcgac gctctgattg tttccggtgc
    23701 attgtcccgg gaactggcca tcagagaaga actgatcctg ctggggtcga cgggcgtgat
    23761 cggcaaaaga ctgccggtga aaaaaatcga aggcggcatc ggcaaactgg ttaaagggct
    23821 cagggaaacg ggaattgaag atgctgaaaa agccatgatg accaccgaca aatatcccaa
    23881 gatcgccatc cggaaaggag tcatcggcgc aaaagatatc accctttgcg gcatcgccaa
    23941 aggcgccggt atgattgagc cgaacatggc caccctgctg acgtatgtca tgacggacgc
    24001 cctgattgat taccgggcgt tgaatacggt atttcatcag gcgatcaatg ccacgtttaa
    24061 ttccatcagc gttgacggct gcatgagcac caacgacatg gcgattattc tggccaacgg
    24121 tctggccgga aataatcccc tggaaaaggt gcaggcgcgt ctgcagttat tccgggacat
    24181 gctcacagag gtgttgatgg aactctgcca ggcggtcgta aaggatgggg aaggcgcgac
    24241 aaaattcatt tccatcatgg tcaaaggcgc cagatcgaaa tccgacgcgc gctgcgctgc
    24301 ttatgcgatt tccaattcga atctggttaa aacagcctgt ttcggggagg atcccaactg
    24361 gggaagaatc attgccgccc tcggcgcttc gggcatatcc atggacaagg agaaagtcgt
    24421 cctgaccatc gccgatctcg cggttttttc gcaaaacacg ccccaggagg tgctgatgag
    24481 cagactgaag gacgccttta agaaagatcg catcgatatt gaagtggatc tgggagcagg
    24541 cgacaaatcc tattatgtct acacatccga tctttcgcac gaatatgtca aaatcaacgc
    24601 ggaatattca acctgagttt atcgaggcgt caaaatattt attttccgat aaaagttatt
    24661 aaaacttgat tatttgtgcg gtttgtgtta attgccgccg gataaatttc acacatcccc
    24721 ggattgccgg tttgttgctt gaaggtcaag ttggccggcc aaggtgaagg ggatggagga
    24781 aaaaaacaaa aaaggagaga gatatgtcag cgatttcaat gaaactttta ctggaggctg
    24841 gtgttcattt cgggcatcag acaaacaaat ggaatcccaa gatgaaaccg tacatcttcg
    24901 gagccagaaa caacatttat atcatcgatt tgcagcagac cgtggggctt ttccagtcgg
    24961 cgtataactt tgtcatcgac accgtcagcg aagggaagga aattctcttt gtcgggacaa
    25021 aaaaacagtc acaggaagcc attaaggaag aagcaacgcg ttgcggtatg ccttttgtca
    25081 atcagcgctg gctgggcggc atgttgacca atttcgttac catcaaaaag agcatcgatc
    25141 gtctgaactc gctgacggac atgttcaatg acgactccat taaagctttt cccaaaaaag
    25201 aaattacgaa actgcagaag gaaaaggaaa aaatggaaaa cgtcttgggc gggatccgga
    25261 ccatcaagtc tctgccggcg gccgttttta tcgttgatcc cggcaaggag catattgccg
    25321 tcaatgaagc taagaaactg agaattccaa tcgtcgctat cgtcgataca aattgcaatc
    25381 ccgatgatat tgattacgtg attccgggaa atgacgatgc catcagggcc atcaaactgt
    25441 ttacctctaa atttgccgat gcagttttgg aggggaaaaa gcgttttgaa gaaaaactgg
    25501 ctgctgaatc gaataaggaa gcggatgccg ccgtggagac caaaccggtg gaagcggata
    25561 ttccggaaag tctggaaatt gatgatagta tcgataaatc ggaaacgtac gccgaataat
    25621 cggaatctgt ctggaattta gaatagcatg taagggagaa aagtaaacat ggaaataaca
    25681 tcaacaatgg tgaaagaatt aagaacgaaa accggcgccg gcatgatgga ctgcaagcag
    25741 gcgctggctg aatcagacgg ggattttgag aaagccatcg attttctcag aaagaagggg
    25801 ctttccgccg cgacgaagcg gtcgtcgaaa gcggccaagg atggaaccat tgcctcctat
    25861 atccacatgg gcggcaaaat cggtgtcatg gtggaaatca actgcgaaac ggatttcgtg
    25921 gcgaaaacgg aagattttca gaccatggcc cgggacatcg ccatgcacat tgcggcgtcc
    25981 aatccgcttt acgttcgtcc ggaggaaatt ccggagtccg ccctggagag ggaaaaggaa
    26041 atctaccgca gtcagttgcg cgaagaaaag aaaccagaga aaatctggga taagatcatc
    26101 gagggaaaac tcaaaaaata ctacgaggat gtctgcctga cccaacagaa attcatcaaa
    26161 gataccgaca tcaccgttgc gacactgatc aacaatatga ttgccaaaac gggagaaaac
    26221 atggttatcc gtcgttttgc ccgttttcaa ctgggtgagg aaatcaaaca ataatgagca
    26281 caaagcagaa ggcggcatat aaaagagtgc tcctgaaact cagcggcgaa gccctgatgg
    26341 ggaaacagtc caccggtgtc gatccggatg tggccaatta cattgccgat gaaataaagt
    26401 cgctttccag aatgggtgtt caactcggca ttgtcatcgg gggcggaaat attttccgcg
    26461 ggattgaagc cagcgccaga ggtatggatc gcacgacagc cgattacatg ggcatgctgg
    26521 caacggtcat caacagcctg gccctgcaga gcgcgctgga ggaacgcggt cttcccacac
    26581 gcgtgcagtc atccatcgaa atgcatgaaa tcgccgaacc gtttattcag agacgggcga
    26641 tccgccatct ggaaaaagga cgcatcgtca tatttgccgg cggcacgggg aatccctatt
    26701 tttccacaga caccgcggcg gcgctgcgag cggtggagat ccgggctgac gtcatcatga
    26761 aagccaccaa ggtggatggt gtttataacg cggacccggt cagggacaaa aaagcggtca
    26821 agtttaataa aataagttat attgatgtct taacaaaaaa ccttaaagta atggattcaa
    26881 cggcaatatc gctttgccgt gataatttat tgcccataat tgtttttaat ttgcagaaga
    26941 agggcaatat ttgcaaagcg gtttgcggac aaaaaatcgg aacttacgta ggagaataat
    27001 catgaaggaa accgtttttc agaaatttaa ggaagacatg gaaaaaacga ttgccggatt
    27061 aagcaaatcg ttcagcagaa tcagaaccgg cagagcatcg gtttcgctgc tcgatggtat
    27121 caaagtcgat tattacggcg ctccgactcc gattgcccag gtggcgaatg tttccgttcc
    27181 tgaaagccgt ctgattttaa ttgttccctg ggatgtttcc attatcggcg ccatcgagaa
    27241 agccattcag aaatcggatc tgggcctgac cccggccaat gacggcaagg tcgtacgact
    27301 gagcattccg cagctcacgg aagaacgccg caaagagata gccaaaaacg tcaaaaaaat
    27361 ggctgaagag gggaaagtga aactgcgcaa tgcgcgcaga gacgccaacg aagaattaaa
    27421 gaaattaaag aaagacaaca aaatatcgga agacgaactt tttaccgcgc aggacgaagt
    27481 acagaaactg atcgatcagt atatcgataa aacggataaa attctggcgg ccaaagaaaa
    27541 agagatcatg gaaatttaac aaacgcctga tacgaaaacg acgatgggga gtttccgact
    27601 ccccatttgt ttttaatacg gtgttttgat tatttctgtc caggcaggat gccattcaaa
    27661 aaaattatcc gggaattgac tgttgaaaaa aaatcagcgg tcgattagga tagaccatca
    27721 ttcaggagca tacgtgtttg gttaaaatcg acgtcaataa attgccccgt catatcgcca
    27781 tcatcatgga cggtaacggc cggtgggcca agcagcatgc catggggaga atccgggggc
    27841 ataaaaaggg agcggaggcc gtaagaacga cggtgcggac ctgtcgggaa atcggcgtca
    27901 aatatctgac cctttttgcg ttttccatcg aaaactggga gcggccggac aaggaagtca
    27961 aggcgctgat gtatctgctt gaagaatatt tgaatgccga actggacgaa ctgcagaaac
    28021 aggaaatccg tctgaccacc atcggagaaa cggatcggtt aaacgagacg gtccgggaaa
    28081 aactcatcga gacgatcgat cgtacggcgc aaaatgacaa gatggttttg aatctggcct
    28141 taagttacgg ggcgaaggat gaaatcctgg cggcggtgaa gactgtggtg cgggacgctc
    28201 aaaccggcag tctcgatatc gatcgcctgg ataaagaaac attcagccgt tacctgtaca
    28261 cggctcagat gcccgatccc gatttgctga tccgcaccag cggagaatac cggatcagca
    28321 atttcctgct ctggcagatg gcctatacgg agctgtattt tacggatgta ttatggcccg
    28381 attttacccg ggaggatctt ctgaaggcca ttgccagtta tcagaagagg gaaaggcgtt
    28441 tcggtctgac cagcgagcaa ctgcaggaat agcatcccgc aaaacaataa aggcagaagg
    28501 tgaaaaatca tggcgaagac acccagttcc ctgatcaaga gatggctgac gggcattgtg
    28561 cttgttccgt tgctcctgct ggtgattttc ttcgggtcgg aagcgatgtt tgccgccgtc
    28621 gtgattctgt tcatttgcgg aggcgtctgg gaatatcatc acatagtgtt cggacaaggc
    28681 tttgtcatgg aaaaaatcga aagccttgct tttgccgttt taattccgtt atgcgttttg
    28741 tccggcaacc atcagtatct gacggcgctc cttgcgctgg cggtcatgct ggtgtttgtt
    28801 ttatttatcg ggtccatccg ggaatcgtct tttgattttc tttccgtcgc caaggtggtg
    28861 ttcggcatca tgtatatccc tttcctgatg tcccatttca ttgccatcag aatgctggac
    28921 cagggtgtct ggtggatcat gtttgttctg gtgctggcct ttgccggtga caccgtggcg
    28981 ctgtatacgg gcaagtattt cggaaaacac aagctggttc cgctgatcag tccgggcaaa
    29041 accgtggagg gactgatcgg tctggtggtg ggcagcaccg ttgcctgtct gatctacagc
    29101 cattatctgc tgccggatgt agcggttgtg catgtggcgg tcatggcgtt tgtcggcgga
    29161 atgatcggac aactggggga tatttgtgaa tcggccatta agagaggtta cggtctcaag
    29221 gatgccggtt cgatccttcc cggccatggg ggcattctcg atcgtctgga ttgtctgatg
    29281 ttcatcgctc cctttgttta ttattaccgg atatttgtga taggttaaat gaaaaaaatt
    29341 acattattgg gctccaccgg ctccatcggc ctcagtgcgc tggatatcat tgagaagaat
    29401 ccgaagcgtt tccggattgc ggctctggct gccggtgaaa atattcaatt gctgaagcgg
    29461 cagatcgaaa gattcaaacc ggagatggcc gccgtaaaaa acaaagaaac ggccgtcaaa
    29521 ctgcgccggg ccctgagacc cggaacccgg attcggatca gttatgatac ggaaggactg
    29581 caggaaaccg cggcctgttc gtccgcggac atcgtcattt cagccatttc cggagcggca
    29641 ggactattac ccacactggc cgccatcgag gccggaaaag acatcgcgct ggccaacaag
    29701 gaaaccatgg tgatggccgg ccggatcgtc acgcgcgcag cggcgaaaaa gaacgtccgg
    29761 attctgcccg tggacagcga acacagcgcc atcttccagt gtctggaagg aagcagaaaa
    29821 gaggatttaa agcggattat cctgacggcg tcgggcggac cgtttttaaa cgttgcggcc
    29881 cgtcagttaa agaaggtcac gttgcgtcag gccctgcgcc acccgcgctg gaaaatggga
    29941 aaaaaaataa ccattgattc cgcctccatg atgaacaagg ggctggagat cattgaagcc
    30001 cggtggcttt tcaatacgtc ttttgaccag atcgatgttc tggtgcatcc gcaaagcctc
    30061 gtgcattcca tggtggagtt tatcgacggg gcctgtctgg cgcagctggg cgtaccggat
    30121 atgaaaattc ccatcgctta tgccttgaca tatcccgaaa gaattgccaa tgatttgaaa
    30181 atactcgatc tcgtgcaggc cggccggctg gatttttaca aaccggatct gaaaaaattc
    30241 ccctgtttga agatcgcccg tgaagcaggg gtcagcggcg gcacggctcc ggcggttttg
    30301 aatgccgctg atgaagtggc tgtggcggca ttgatgcagg aaagactctc ttttgccgat
    30361 ctgcccaaaa tcatcgaaaa ggttctcgat gcgcatgatg ttatccccga tccttcaatg
    30421 gatgacatat tgcaggcgga tgccgacgca agacaacaaa ccgaaaaaat aataggaagg
    30481 ataacaacgt gaccacttta atttctttta tcatattgct ggggattctg atttttgtcc
    30541 atgagctggg acattttctg gtcgcccgga tgtcgggcgt cggagttcaa aaattctccc
    30601 tgggcttcgg accgaaaatt atcggcagga caatcggtga aacggagtat gtgctgtcat
    30661 ggattcctct gggaggctat gttaagctga tgggtgaatc cggcaccgaa gaattatcgc
    30721 cggaagatga aaaacgatca ttcgccaggc aaaatgtctg gaagcgaatc atgatcgtcg
    30781 cagcaggtcc cgcgttcaat tttttactgg cggtagtcat tttcattgcc gtgatgatgt
    30841 acggtttgcc gacgctgacg tcggatatcg gcgaggtcca gaaggattcc gcagcgtatc
    30901 aggcgggcat gcttaacggc gataaaatca tttctctgga cgggaaaaag attgccttat
    30961 gggaagagat ccgggatgtc gttgcccggg ctaaagacag acagatcgaa gccgtcgtgc
    31021 agcggggaaa agaaacagtg cggttgcaaa tcacaccgaa attgtccaaa tcgaaaaatc
    31081 ttttcgggga agacgtttct gcgtatctta tcggggtgtc tcccgcggga caaaccgtta
    31141 tcgagaggaa aagtcccgtt gatgccgtcg tttcgtcggt ggccaaaaca tgggaaatca
    31201 gcaaattaac ggttatctcc gtaattaaaa tatttgaagg cgtgatttcg ccgaaaacgc
    31261 tgggcggtcc catcttcata gcgcaggttg caggcgctca ggtcagggaa ggcatcattc
    31321 cgtttgttct cttcatggcg attttgtcga ttaatctggg ggtgatcaat cttttcccca
    31381 tcccggtatt ggacggcggc catatcctgt tttacctgat cgaggtcgtc accagacggg
    31441 aggtcagcgc caaggtgaga gagatgtccc agcaaatcgg gtttgtcgtt ttgctgatgc
    31501 tgatgctttt cgttattttc atcgatatcg aacggctaaa cataaaattc gtcaacgatg
    31561 ttgtaaaatt ttttaaatga tatgcggatc ctggcgtttg acacctcatc ccaaacggct
    31621 tccgtggccg tcctgcaaga tgacgatatt ctctatgacg tgatcattca taccggcttg
    31681 aatcactcgg aaacgctttt gcccgccatt gaccaggcct gtctgcggac caaaatcccc
    31741 cttcccgaat tcgatgtttt tgcctgcacc ctcggtcccg gatccttcac cggtctgcgc
    31801 atcggcgccg gcacgctcaa gggattcatg ctggccaccg gcaagccggc ggtcggtgtt
    31861 tcctccctgg ccgccattgc gctgaacgcg gagaaaacct cgaaaaaaat atatgcgctg
    31921 atggatgcgg ggcgcggcca ggtctacacg gcctgcttcc aatacggggc cggcggcatg
    31981 atcgaacaac aaggcggcga acaggttgcc aatccgtcgg acatcgttta tgacgccgat
    32041 gccgtttacg tcggcagcgg ggttatcaaa tacggggccg catttatgcc cgaacggcaa
    32101 aacggaattc tttcaacggc aaaacagcat tgccgcgctt cggccgtggc tgttttaggc
    32161 aaagcaaaat atgaacgcgg ggaacggctg aatgccgaaa actttgtgcc gttctatttg
    32221 cgctcggcgg atgccgatca gaaaaaatcc tttttcggga aatgacttcc cgcaggcatc
    32281 atgacgcttt gatcttgctg agcagttccc agacttttct attgttgggg ttctgcgaga
    32341 gaacgagctg caaccgttcg acaatatcat cgattttaac gatattcgga ttggataaaa
    32401 acagggaaaa ataggcattg gccagattat agttggtatc ctcgtgaagc ggattcacct
    32461 tcagcgcctt gcggtaagtc gaaatggcct ggatcagatt gttggtgatt tcataaaccg
    32521 atcccagatc attccaggca tcggtatttt cgggttcgat atttaaaact ttctggaaac
    32581 tcttcagcgc tctggcgtaa tccccgtcct gaaaatagat tcgtccaaga ttgtggatgg
    32641 ctttggtatg cttggggttg atctcgatga tgttttcata ttcttccgcc gcctcgtcat
    32701 acatgtcgtg tctgtcgtaa agggttgcca ggcaaaagcg cggttcgatc gcctgaggtg
    32761 cgatgtcaat cgctcgggta tacagttcca cggcgttgtg ataatcgccc tgcatttcca
    32821 gaatatgacc gatttttaaa atagcttcga tataatcgct tttgacctcc agggcttttt
    32881 cgtaggattt ttttgcagaa tccagcatat ccatcttttc ataaacatag cccagatgga
    32941 tataattctt cgggttgtgc ggttcaagtt cgatggtctt ttgaaagcat ttcaaggctt
    33001 gatcaaattc tttgttttcc aggtagatga aaccccagtt ttcataatct gaaggcgaat
    33061 agttgtcttt attgatattt ttggaatttg cgatattcat ttcgtgatga tcaagacacg
    33121 cccgattaag acataaatat atttagattt tcgaattttc atccatttga aatcaaatca
    33181 ctgcatgtgt gcagggctgt tgaatttttt caaaatgaca ttcttgactt caaaccataa
    33241 agataccgga tcagcaaaag aagaatgcgt taactgcgga tttcccttta aaatttgcgc
    33301 cgaccagtag cccgctaatt tgttcttatt ttccttgttg gatgactgcg cctgccggat
    33361 gacatgcgac ggaatcgcca gtttaatgga ctgctgaccg atggtgacct cttcaaaatc
    33421 ttcctcttca actgcctcca tcggcatcgg ctgctcttca taagactgtt gagccgtgtc
    33481 cgcagcgcgg acgccttgcg gaggagcaac attacccgga gcgctcaccg gaggcgggac
    33541 aacgcttgcc gaaggtttcg gcggtactgg aaccgcctga ggacggaccg gtatcggctc
    33601 ttcctggcgc acaggtgcgg tatatgccgg catctcttcg ggtgcggcca ttctcggtct
    33661 catcggcgca ggctcttcca tgcgcggcgg agccggactg acaggcggct gctcgggcat
    33721 caccggcctg ggcctggccg gaggcgcttc atccatgaca ggcggcgctg tcggtctgag
    33781 cggcatctct tcgggtgcgg ccattctcgg tctcatcggc gcaggctctt ccatgcgcgg
    33841 cggagccgga ctgacaggcg gctgctcggg catcaccggc ctgggcctgg ccggaggcgc
    33901 ttcatccatg acaggcggcg ctgtcggtct gagcggcatc tcttcagggg cagccattct
    33961 cggtctcatc ggcgccggct cttccatgct cggcagagcc gggctgacag gcggctgttc
    34021 aggtggagcc attcttgatc ttggcggcat caggtcttcc atgcccgggt gaagggggcg
    34081 ccgcatcggc ggctgttcgg gcataacggg tctgggcctg accggaggcg cttcatctat
    34141 gacgggcggc gctgtgggtc ttgccggcgg ttgttccgga ggagccattc tcggtctcat
    34201 cggcgccggc tcttccatgc gcggcggcgc cggccggacc ggcgcggaaa cagccggtgt
    34261 ctctttttcg gggaaagcag gtcgcgacgg tttcggtatt ctttccggct cctgcttgcg
    34321 gggagcttct gcggcaggtt gtgcttccgc ggcaggcgct ttgcgttttt gagcggatgt
    34381 cttgcttaat ttctggaaac gatcggcgat ttgctgggcc acatcaccgg atatggtctg
    34441 aaattcattc gtttcacccg cttttatgca caacttgcag attttgttga tcagacgcgg
    34501 cacgccatgt tcggaaaatt cccagatcac cgggatggca tcgtcgtcga aaatttttct
    34561 gtccgtgccg gcgattctga gccgggattc gatatacgtt ctgacggctt ccggagaggg
    34621 tagcttttcc agatgaccgt atgtgccgat acgttgaaag agatttgccc gcttgggatg
    34681 ttccagcgtt ttggcgaatt ccatctggcc gacgagcagc agagtgaaaa gatttcgttc
    34741 atcatcctgc atattggtca gaagccgcag atcgtcaaga ttggaaggag acagaacgtt
    34801 ggcttcatcg atgacaatga cgattttcct gccctgatct gccgtttcaa acagaagacg
    34861 gttgaatatt tcaagcaaat cgaccttgtt tttgacttcg cactgctttc cggtaatctg
    34921 accgatgatt tcccgcaata actgaacaaa cgagagcgcc ggattggtaa ttaacgccac
    34981 tttatatttt tcaggttcca gcgagtctat gatcacgcga agagatagcg tttttcccag
    35041 gccgacttcg cccacaataa ccgtaaggca ctcgttgcct tctttgatgg caaataaagt
    35101 ttcggagatg acattttcca tggattcatg acaatccaca tacatggagg catcgggaac
    35161 attgtcgaaa ggtggcttag ttaaattcca gtaatcacaa tacatctttc ttctcctcgg
    35221 tttcgccttg taaaagcaaa aaaattattt tcagccaata aaacacgcac ccatttattt
    35281 gtcatcgctt ggctgcatga aatcaacctc gctgctcaaa tactccagga tatcttccag
    35341 caggaacctt ctcattcttc ccagtttgta acttttaatc ttgttttcat gaaccagctt
    35401 ctgcaaaaag ctgcggctga cctgaagcat ctggcacacc tggttcggcg cgatcattcg
    35461 caaaagcaac gaatcgctga atcggtaatt cagggacagt ctcccttcag gcgttacctt
    35521 gatatccaaa gcgggatcgc ttttcttgcc gctgagcagt tctttgatgt attcggattg
    35581 cagtaaaaga tcggattgct ccttggtcat ggtgatggcg atgacggacg acgcattgtc
    35641 cacgaattct tcgacaattt ttcgttcggg tatcttgatt tcatgctcca tccccacact
    35701 gcggattgtc aattcaggcg tcatgctttc ttttgttgtc cgggctgtat tctccgataa
    35761 tcccgcgttg tttttaacgt cgacgcggct gacggcaact tcgggggcgg ccgtttcact
    35821 gatggccggt tgcgtaaccg ttacttttac ttccggttgt gtgtattctc gacgcctctt
    35881 tacaatgcaa atcttttcca tatgactcct gtggtctggg atcgctataa attattcacg
    35941 gaaatccgtc tgcaaaacag attgatccgg tacaatacaa tccatcaata aaaaatcagt
    36001 ttctctcctc taatcagttc tcagccggga aaacatttgc ggtctattac aatcaaaaaa
    36061 agcttcacat tcccaatctt agctggaaac tgtagaattt aaatttaatt tagtgcaatg
    36121 tagcttattt attaagtaaa atcaagacaa ttttgttcat ccatgtcgtt tcggcatgcg
    36181 tgtagcctga gtttcaaaaa aatcacctat gaaaaaatat ttattttacg gttaagcgtt
    36241 ttcggcccag atgtatcgga taaaatgtcg ccagcagaca caggaaaatc gccagcgcga
    36301 aagaaagcac cgcccacagg attttcaggg ccgtgagatt ttttccgaga acgtccgcca
    36361 tgaaaatggt atagaccggt ccggcctcca ggatgatcac aagcgcaatc agaccgaagc
    36421 ttaaaatcat aaaaagcagt cccccaaaac ccgttacgat gttggccgga ttttccgatt
    36481 tgaaatcggc gtaaacggct ccgagtcccg tggccagtcc gacaatggcg ggaaccagaa
    36541 aaaaaacggt caggacggac aacagcatca tgaaatccga aacgttgagg agaatgttgg
    36601 acgcgaccac caggccttcg gccatcatca tcagggggat ataatagaga aaaaatttga
    36661 tccagagaaa attgccgatg cttaccggtg cggattgcac aatccagaac gcttcgcttt
    36721 ccatgctgac ggccggaaag acaaaccggg ccgcaacggc ggcgagaacg gcagcggcca
    36781 gcccgatgtt caaaaaagaa aacacgttct gcagataaat ggttttaatc ggataactct
    36841 ccagcggcag tacggaaaaa ttgtagatgt aaatcacaat cagcgccgtc atcaggaaaa
    36901 gttgcggcca ctgggaggaa tccctgaaaa atgtccggat ttccttgacg gcgaacgctt
    36961 tggattcacg cggcaggaag ttcagaagcg tttcccagga gaatcctttg taccgcaaag
    37021 gcgcaaacag acgacggggt gtcatctgcg attttgaaaa tcccggaaaa tacatgttcc
    37081 gggcaagcat actgctgata aaaacgagca tacaggcaaa ggtgaccacc aggcccgcat
    37141 tcagaagcgc cgttgcgcgg tcgtttttca aagcggcgag gatggcgtcg gtgatccacg
    37201 ttgtcggcag gaccggtgaa ctgggcgtct tcagcgcatt cagatagagg accaccgatg
    37261 caaaactgtc gggattgacc agctgttcgg gccgtgtgaa gcgcagaacg aaaatcagaa
    37321 caatcatcat cagcgctccc agcagaatca gaatggttct gatgcgtccg gcaggcagaa
    37381 ctttcgatcc gaacaacacc aggatcccgc tcagggcggc ggcgatcaga cacatgaaaa
    37441 tcatgacggt cgtaaaaatg gcgtaataga agatgccggt gtgaaaaatg atcccgtacg
    37501 acaggaaaac cggaagactg aaggccagaa tcatccagga actgtcgaaa gcgctgatga
    37561 tccagcgcga aaaaaagatg tcgtttgcgg ataccggcag agaatgcaga agcggcaaat
    37621 cctgactcag gtaaaggtgc gagaggcaat tgatgatgtt gctgaacaac aaaagcatga
    37681 agaaggtcaa aataatcatg gagacaaact tcatggccag aatgtctccg aaatcctgca
    37741 cgctgtggaa atacagcagg acgcggtaga aaatcatgaa gatgcccgcc cagaacaaaa
    37801 gaccgagtcc gccgaacatg acaaggcgcg accagacatt tttcctgttg ccgcgggtca
    37861 gactgttgag cgaagaccgc acgcgcggtc cgatcaggga taaaagggtc ttcatagaaa
    37921 cgtgttctct ttaatgatcg tcagaaagac ttcctcaagc ctcgcttcgc cgagtcgggc
    37981 gatctgcttt aattccttga ggctgccctc ggccagcagc gtgcccttgt gaatcagccc
    38041 gatacggtgg cagagatctt cggcaatact caacgtatgt gtggaaacga aaatggtggt
    38101 ttgacgcgcc gcgaggtctt tcagaatatc cttcaccatg tgcacggctt ccggatccag
    38161 tccgaccatc ggttcatcga tgatgagaat ctgcggatcg tgtaaaagcg cggcggcgat
    38221 gatcagccgc tgcttcatgc cgtgggagta ggcttcgatc agttcatccg cccaggtcca
    38281 caaggcgaat tgctgcagga ggcgtttggc tttttcaggg aaaagaccgc ggcggacgtt
    38341 atacagatta gcggagaact ccagaaattc catgccggtc agtttttcgt acagaaaagg
    38401 tctatcggga acaaaaccga tcatccgttt tccttcgacg ggatttcggg ccatgtccac
    38461 accgccgatc acaatactgc cggaagtcgg cgcaagaatg ccgcccatca tgcggatggt
    38521 tgtggttttg ccggcgccgt tgggaccgat aaagccgtaa atttcccccg cgttcaccgt
    38581 caggctgagt ttgtccacgg cgacggtcgt tccgtaatcc ttggtcagtt cggataatgt
    38641 gatcatctcg tttgtgacag cacctccagg tttgttttgc cgatcacccc gattaagcgg
    38701 atctgctgat cggtttcgcc gcgcagcagt ctgatatgcg tgacatccgc gggaatctgg
    38761 ttgaacaccg cgtcggcggt tatccatcgg ttgatgtaaa gcacgcacca ggcgtgataa
    38821 taaaatcgtc ccctcagata caccagaccg gcttcagacc gggccggaat gccggctgcc
    38881 cgcaacaggg ccaccgtcag aacggcatgt tcgttacagt cgccggatcg gtttttcaga
    38941 acctccaggg cattcggcac cgacagagcc ggcttttttt cgatattcag aaaaacccag
    39001 ttgatgattt tccgtgcttt gacaaaagcg gaatcctccg gacggacgat tttcccgatg
    39061 agcgccttga tttgcggatc atcggattga atcaatgcgg cgggtttcag aaatacggcg
    39121 atatccgcag gccaaccgcc tgcaggacct ttatccgcgg gatcttcacg cgtgatggtc
    39181 agaacgccat cgtgataatg ctgtctatcg ccgtcgagat ggagctgacg ctgcagcggc
    39241 ggggtaatcc cgctgatcct gatttgaagt ttgcggagtt tttccggatc ggcgatttcc
    39301 acattggagg ggatggaagc aatttgcgta aaatccacgc ctgcgctgcc gtcgatttcc
    39361 gccagcgcat tttcccggct tgttttttcc atggaaagac ccagcatgcc ggtttctttt
    39421 aacacctgtc cgtctttgtc cagccatgcg cagtgtttgg cgcccataaa atcggcgcag
    39481 tattttttca gaagcaggcg attgcccatg atcggaataa tttcatcggc gttgcgcgtg
    39541 acgttgatcg tccggaccga catgcttgac ggatcgaaaa tgctgaatcc gcgggttgtg
    39601 cccttatcca tgccggcgtg aaaggccgcg tcgtagatgt tgccgctgat atgcggcagg
    39661 gcctgaagcg gcatttcatt tttttgctga cggtttgggg caccggtgta aaggatcagt
    39721 ttgttgtcgg ccacacggcc gcggatatga aactgaaaga gactggaatt taatttgaaa
    39781 gtgaaggagg aaagggtcat gtccgcgttt agctgtcctt cagtggcgag attcagcgcc
    39841 tgcggcatcc ccatggtgct gatttccata aaaacatttt cgttgaaggc aaatccgttt
    39901 tcatttttcg tcagcatccg gtggacaaat ccgattttcc ggtcgttctg gaaaatcgtc
    39961 atccagatat cctgcggttg ttgcggcggg accattttta tccccgcgac cggaggcgtt
    40021 tgcgtctgaa aaaaatcaag acggatcaaa agcaaaacgg caaaggcaat tgtgaccaga
    40081 attccggcgg ccattggcca tttaaacggg cgcgtcatgc aaaaaacctt aaaaaataat
    40141 aatcattatc gattaatata caggagacac cggagcgatg caataacaaa catgggagga
    40201 aatccaacat gtttttgtat aaaaattaaa gatttattga caagcacggt caattgatat
    40261 atcagaaaaa ctcgtttaac gacaagacgt ctgacgggaa atgcagatcg tgattgccgg
    40321 ttatgtggat gtgatattaa aagcttgttt ttattcggaa aagataagta tttatgaagc
    40381 atgacagtat tatcgccgct gaaggatatc cttttgtagt tttttccctg attgtcacag
    40441 tttttgtcgc ctttctgggc ggtaactggt ggttgatcct ctttttcgct cttgtgacgg
    40501 ttttcatcct ctgttttttc cgcaatccgg agcggtcttt tccggaagac gaaaaggttt
    40561 taatcagtcc cgccgacggt aaaatcatca aaatcgaaga ggtggacgtc aacggcaccc
    40621 tctcggggaa attcaaaaaa atcagcattt ttatgaatgt tttcaatgcg cacgtcaacc
    40681 gggctcccta tgacggcaaa attgaagccg ttcattatca tcccgggaaa ttcttttccg
    40741 cgaatctgga taaagcgtcg gaagacaacg agcgcaatga aattctgatc cgcacgccgg
    40801 acaaccgcac ggtctgggcg gtccagattg ccggtctgat cgcgcggcgt atcgtctgct
    40861 gggtgacgcc ggggatgcct atccgcaagg gggagcgcat cggcatcatt tgtttcggat
    40921 cgcgggtgga ggtctatctt cccgtggatt ccgccatatc ggtcaaattg aaagataaag
    40981 tcaaggcggg ccaaacggtt ttaggatatt tatcatgaac gtggaaaatg cagcgcggaa
    41041 aatccgcatg aaaaagggca tctacctgtt gcccaattta ttcacctcgg ccagcctttt
    41101 ttgcggattc tactccgcca tcgcatcgtt caaggaacat tttgtcacgg cggcaatcgc
    41161 tattctggtc tccgtcatct tcgacgggct cgacggccgt gtcgcccggc tgaccaacac
    41221 caccagcaaa ttcggcgccg aatacgattc cctggcggat gtcatctcct tcggcatagc
    41281 gccggccctt ctggcctaca gctggtcgct ttccgtttac ggcaagtggg gctgggtggt
    41341 cgcatttctg tttgtgctgt gcggcgcttt gagactcgcg cggtacaaca ttcaaatagg
    41401 catcatcgaa agcaaggtct tcaacggcct gcccattcct gccgcggcgt cggtcattgc
    41461 gaccaccgtg atcttcttcg actatgtcgg agcggaagga aaattccaca atatcttcat
    41521 catggtcttt gtcatcatga tctctttact gatggtcagc agcgtcaagt attacagttt
    41581 taaagacatg tctctgctgg tccgcaaacc cttcacgatt ttcttctggt caaccgttct
    41641 gctcatcatc attgtcgcgg aaccggagat aatgtttttc gtcatcatcc tgggatacgc
    41701 cctgtcgggg cccgtctggt ggctgatcaa gtttggcttc ggcaaaccga aggccgtccc
    41761 ggaaaacaaa aacaatgcag cgaatggaaa accgtaaggg catcatcttt aaaaaactat
    41821 ttttaaaccg gatatgagaa ttacaggagg aaaagccagg ggaaagatgt tgcttttccc
    41881 ttcgggatca aaggaaaggc cgaccaccga ttttttacgg gaagcgcttt tcaatttgac
    41941 cggttctttg aacgaaaaaa catttcttga tctttttgcc ggatcgggca gtgtcggcct
    42001 ggaggccgcc agccgcgaag cgcgccagat cgttctggtg gagaacaatc aaaaacttgc
    42061 cggcgtgatc agaaagaata ttccgaacgg cttgcaggac ggacggttga ccatcatggc
    42121 aaccgatgtg gcatttgccc ttcgcgatct ttttagaaaa cagtgttcgt tcgacatcat
    42181 ttttgccgat ccgccgtatc accgcggtct ggtcgccgtg accctggagc tgttgggcaa
    42241 atacccggtc ctggcgaaag gcggtatcct ggtgatgcag cattccgtga aagaaccgat
    42301 accggaaacg gtcttaggaa tttttcaccg gaccgatcag agaaaatacg gcgaaaacgc
    42361 cttaactttt tttaaaatgg agagagaata atgcaggaag attttacaaa aagaatagcg
    42421 atatacccgg ggtccttcga tcccatcacc aacggacatg tggatatcat caaaagaggg
    42481 aaacggatgt tcgatgagat catcgttttg atcgcttaca atcccaataa agtctatctg
    42541 ttcaacattg aagaacgaat ggaaatgatc cgcgaaatat tcggggagac accgggcatc
    42601 cgcgtggatt gctactccgg tttgctggtc gattatctca aaatgtcggg ggccaacatc
    42661 attctgcgcg gcatgcgggc gttgtccgat ttcgaatatg aatttcagat ggcgttgatg
    42721 aaccgacgcc agaccaaaca cattgaaacc gtctttctga tgagcggctt taaatggttt
    42781 tttaccagtt ccaaactgat caaggaagtg gcgagtctgg gcgggtccgt caagggactg
    42841 gttccggaca atgtctatca gcgattaacg gaaaaataca ataccagggg gtaagttgtg
    42901 cgattatcag cgagagtagg cagaatcagt ccgtcggaaa ccttggcgat cacggccaaa
    42961 gtgaatgctt tacgggctca gggaagagac gtcatcggtt tcggcgccgg tgaaccggat
    43021 ttcgataccc ccgataatat caaagatgcg gcgatcaagg cgattcagag cggattcacc
    43081 aaatacacgc cggtgggcgg aacggatgaa ctcaaggacg ccatcatcaa caagctcaaa
    43141 cgcgacaacc gtctggaata caaacgctcc cagatcgtcg tttcctgcgg agccaaacac
    43201 accttataca atctcgcgca gatttatttt gacgccggcg acgaggtcat cattcccgcg
    43261 ccgtactggg tgtcctatcc ggacattgtc accctcgcgg acggcacgcc ggtcatcgtc
    43321 aaaaccgaag agaaggatgc ctttaaaatg aagccggaac agctggcggc gtccatcacc
    43381 gcccgcacac gggcgctgat tctcaacagt ccgtccaacc ccacgggagc ggcgtattct
    43441 cccgacgaac tcaaggcgat tgcgcaggtt ctcctcgaca aggatattct gatcatcacc
    43501 gacgatattt acgaaaaaat cttctacgcc ggatttccgt ttgccaacat cgccaacgtc
    43561 gagccgaaat tgaaagacag ggtggttgtc gtcaacggcg tgtcgaaaac ctatgccatg
    43621 acgggatggc gcatcggtta cgccgcggcg gcggaagaaa tcgcctccgc gatcagcaaa
    43681 attcaaagcc agaacacatc caatcccaca tcgattgccc agaaagcggc ggtggaagcg
    43741 ctgtcctgcg atcagacgat ggtgggcgat atggcggctg aatttaaaaa acgcagggac
    43801 tacattgtcg cggccctcaa cgaaattccc ggcatcagct gtttctcgcc ggaaggcgcc
    43861 ttttatgttt tccccaacgt gtccggcatc tacggacgtt cttgcggcgg caaggtcata
    43921 aacggttcgg ccgattttat tgactatctg ctggatgaag ccaatgtggc gggcgtgccc
    43981 ggagcggcat tcggcagcga cagccacatc cgtttatcct atgccacctc cctgaaaaac
    44041 atcgaggaag gcatcaggcg gatcagggaa gcggtttcca aactcaccta attaggagca
    44101 caaatcaatg ggcggcatct ttggagttgt atcatcggca aattgcatca aaacgctttt
    44161 ttacgggacc gattaccatt cgcatctggg aacggaacac gcgggcatgg cggtgctcgg
    44221 gccccaggga ttttacaaga ccgtgcacag cgtgtcccag gttcagttca aatcccgttt
    44281 tatcgatgaa tataaaaaaa tggacggatt cttcggcatc ggggccattg atgacgatac
    44341 gcctcagccg ctgattttcc gttcgcggtt cggcgtctat gcggtggccg cggtgggact
    44401 gatcaacaac aaaaatcaac tggtcaatga gctgctcaag gaaggcgcca acttcggaga
    44461 aagggccggc ggagacatca ataatgcgga agtcgtttcc tgtctcatca atcgcggcga
    44521 caacatcgtc gagggcattg cctccatgcg cgccgtgatc gaaggatcca tctgcgccgt
    44581 ggtgatgacc tcggaaggca tttatgccgc ccgcgacaaa ttcggacgat tcccgctggc
    44641 catcggcaaa agcaaaaatc cgcaggatca atcctacgtg gtggccaccg aagccagctc
    44701 gtttgaaaat ctcggctatg aactgacgca gttcctcggc cccggcgaga ttgttcttct
    44761 gaccaccgac ggcgttaagc cggtcaaacc ggcgggcacc gagaaaaaaa tatgttcgtt
    44821 cctctggatc tataccggct atccatcctc ctcgtacgaa ggcatcagtg tggaaggcgc
    44881 ccgggagcgg tgcggcgcct atctggccaa acgcgacaat atccaggcgg actttgtctc
    44941 cggcgtcccc gattccggtg tcggacacgc cctggggtac gcgatggcgt cgggcattcc
    45001 ctaccggcgg gcgctggtca aatacacgcc cggttacgga cgaagctaca cgccgcctgc
    45061 gcaggaaatc agagacctgg tggccaccat gaaactttgc gccgtgcggg aagtgatcaa
    45121 cggcaaccgg atcatcatct gcgacgactc catcgtgcgg ggcacgcaac tgaagaatct
    45181 gaccattaaa aaattatggg acaatggcgc gaaggaaatc catgtgcgtc cggcctgtcc
    45241 gccgctgatg ttcccctgta tctatacctc ctcgacgcgg acaaccggtg aactggcctg
    45301 ccgcaaggcc attcgcgcgc tggaaggaaa agaaatcgaa catccgtcag agtatctgga
    45361 tcatcactct gagaaacacg cgaaaatgat cgattggatt cgccgggatc tgaacgtgac
    45421 ctccttgaaa tatctggcca ttgacgatat gatcgcggcc atcggacttc cggcggacca
    45481 gttgtgcctg cactgctggc tgggcaaata agcgtttgat gacggcctcg tttccgcatc
    45541 gtgacggacg gtttaccgga tcaattcaaa tgtcgtcatt gacttccgat tcaatcaatg
    45601 gaaaaatggc aactgtggaa cattcatcgt ttatcagaag gaaaaaaaca aaacagattc
    45661 atctcggggg cgtggcaatc ggcggcgatg ctccggtgat tgttcagtcc atgaccaaca
    45721 ccgacacgag gaatgtcgag gcgaccgtcc gacagattaa agccctcgaa aaagccgggt
    45781 gcgaaatcgt tcggattgcg ctgccggacg acaaagccgc agcctccgtt tccgccatca
    45841 agaaaagaat ccggattccg ctgattgcgg acatccattt tcatgaccgc ctggccctgg
    45901 aagcgatcag gcagggcgta gacggaatcc ggattaatcc cggcaatatt gcgccgggca
    45961 aaatgggcgc catcatccgg ctggcccggg aacgccgggt ggttatccgg atcggcatca
    46021 actccggatc gctgcagaaa gacctgctga aaaaatacgg cggcgccacg ccggaggctc
    46081 tggtggaaag cgccgtgcgc aatttggaac tgtttgagga ccgcggattc gatctgatca
    46141 aattatcgct gaaatcttcc gacgttcccg ccacgatcga agcataccgg gccattgccg
    46201 cccggacgga ttacccgttg catctgggcg tcacggaggc cggctcgctc atcggggctg
    46261 cgatcaaatc ttcgctgggc atcgggacgc tgctgtacga gggtatcggc gacaccctcc
    46321 gggtgtcggt caccggcgat ccggtttcgg agatggggct ggcgtacggc attttacgcg
    46381 cgctgggcat ccgccgtgtc ggcgtggaca ttgtttcctg cccgacctgc ggccggtgcg
    46441 aaatcgatct tccgagcctc acgcgccgca tcgaaaaagc cctggccggc cggaaggact
    46501 atttaaaagt ggccctgatg ggctgcgtcg tcaacggtcc gggcgaagcg gcggacgccg
    46561 atatcggtct ggccggggga aaagggcagg ggctcctttt caaaaaagga aaagcttata
    46621 aaaaagtcag agaaaaagat tttgtgaata ttttactcga agaaatcgat acgatcattg
    46681 aagaaaggaa aaaggattaa tgcgttattc agaaatgttt ttaccgaccg tccgggaagt
    46741 gccttccgat gcggaaatca tcagtcacca gttgatgatc cgggcgggat tgatccgcaa
    46801 actgaccagc ggcatctatt cctaccttcc gctggggtac cgggtgatca gaaaactgga
    46861 gcagatcatc cgcgaggaga tgaaccgggc gggcgcgcag gaagtgcatc tgcccatggt
    46921 tcaaccggcg gaaatctggc aggaatcggg gcggtggact tattacggca aggaacttct
    46981 gagactccgc gaccggcatg atcatgaata ttgtctgggg ccgacgcatg aagaagtcat
    47041 tacgggcctg gtgcgcaacg acgtgaaaac ctaccgtcaa ctgccgctca acctgtatca
    47101 gatccagacc aaattccgcg acgaagtccg tccgcggttc ggcgtgatgc gctgccgcga
    47161 gttcggcatg aaagacgcct acagttttga tgtcgatgaa gagggcgccg agaaaagtta
    47221 cgaaaaaatg tttgctgcgt ataacaatat tttccggcgc tgcggtctgc aattccggcc
    47281 ggtggaagcg gattccggaa gcatcggcgg caagtattcc cacgagttca tggtcatggc
    47341 cgactccgga gaagacgcga tggtgttttg cgaaaaatgc gcctacgcgg cgaatctgga
    47401 aaaggccgaa gtggcctgtc ccgagaaaaa aatcatcttt gaagacgact ggctgcccct
    47461 ggaggacgtg gatacgccgg atgtccgcac catcgaggaa gtcagtgcct tcttgaaagt
    47521 cacgccgcag aacatcgtca aaacgctgat cttcaatgcc gacggcaaac cctgcgccgt
    47581 gctgatccgc ggagatcacg aggtcaacga ggtcaaggtt aaaaattatc tcgccgcggc
    47641 cgaactggaa ctggcctccg acgacatgat ccgggaagtc accggggcgc cgcgcggttt
    47701 cgccggtccc gtcaatatca aagccccgat tcttgtcgat tattcggtga tcgatatggt
    47761 gaattttatc acgggaggca accggcagga tggccatctg aaaaacgtga atgttggcag
    47821 ggatttcaaa gtcgaagcgt tcacggacct gagaattgtc aacgccggcg atccctgtcc
    47881 gcgctgcgga cagcccatca aaacggcgcg cggcattgaa gtcggccatg tgtttaagct
    47941 cggcaccaaa tacagcaagg ccatgaaggc ggtttatctg gacaaagacg gtcaggaaaa
    48001 aatcatgatc atgggctgtt acggcatcgg tgtcggcaga accgttgccg ccggcatcga
    48061 gcagaatcac gacgacaacg gcatcatctg gccgatgccg ctggcgccct accaggtcat
    48121 catctctccc gtgaatatca atgagatcac catcagggaa accgccgaat atctctataa
    48181 tgccatgatc acggaaggcg tggaggtttt gtacgacgac cgggatgagc gggccggcgt
    48241 caaattcaag gacgccgatc tgatcggtat tccgctgcgg gtaaccgtcg gccacaaaaa
    48301 tcttcaggac ggcaacgttg aactgaagat cagaaaaaca ggcgccaatg aattgtgccc
    48361 gctgcaggac atcgtcggca gggtccggga gatcatccgg caggaattga atccggatat
    48421 cgcctagccg cgaaacgaga gggttgaaga gttcatgctt cggatcaacg acattattga
    48481 aaaagctcag acctatcttc cggcggaaga tctggaaatg atcgagaagg cctatattta
    48541 ttcggcatcc gttcatcagg ggcaggtccg gttgtccggc gagccctatc tgacgcatcc
    48601 gatggaagtc gccggaatcc tgacggacat gaaaatggat gccgcaacca tcatcagcgg
    48661 cctgctgcat gacaccattg aagacaccct gactacggcg gatcaactgg agcacgaatt
    48721 cggaaaagac gtctcctttc tggtcggcgg gctgaccaag ttgagccgca tttccttcgg
    48781 gtcgcaggag gagcggcaat cggaaaactt ccgcaaaatg attctcgcca tgtcgtcgga
    48841 tatccgcatt ctgatggtgc gcctcgccga ccggattcac aacatgagaa cgctgcagta
    48901 tcaccccaag gaaaaaagga tctacatcgc gcgcgaaacc atggatattt acgcgccgct
    48961 ggccaaccgg ctgggcatca actccatgcg gatggagctg gaagatcttg ccttccagta
    49021 catcgcaccg gacgcctata acgacctgac cgccaagatc gtcaaatcga atgaggcccg
    49081 caaccgctac accgaggaag tcaaaaatat cattcagcag gaaatggaga aatattccgt
    49141 tgcctgcaaa gtggaggggc gggccaagca cctctacagc atttacaaaa aaatgcatga
    49201 acagcacatc aatttcgacg aggtttacga tctgatcgcc ttcaggatca ttctcgatgc
    49261 ggacaaggaa aaatcctgtt atgaagtgct gagcatcgtg cacgcgctgt ggaagccggt
    49321 tccgggacgg ttcaaggatt acatcgccat gcccaaggcc aacaattacc agtcgctgca
    49381 taccacggtc atcgggccct acggcgagcg ggtggaaatt cagatcagaa cccggaacat
    49441 gcacgaatgg gccgaaaaag gaatcgccgc acattggcgc tacaaggaag gcggatccta
    49501 caaggacaac gaagaagacc agatccggaa actccgcgaa gtgctggaga tccagcagga
    49561 catcaaaaat ccgaaagaat tcatgtccga tctgaaactg gctctctttc cggaggaagt
    49621 ctacgtattt acgccgcacg gcgacgtcaa gtcctttccc aagggcgcca cgcccatcga
    49681 tttcgcctac agcatacaca ccgacgtcgg caatcagtgt atcggcgcca aggtcaaccg
    49741 cagcatcgtg ccgctgcgct acaagctgca aaacggcgat cgcgtggaaa tcatcacgca
    49801 ggcgggacac catcccagca aggactggct caaaaatgtc gtgaccaccc gggccatcac
    49861 caagatccgc aattacatta acctggaaga aaagaaaaac agcatagttc tgggacggga
    49921 gctgctggaa aaggaattca agcggcataa tctgaaattt tccggcattg tcaaatcgga
    49981 cgacattaaa aaaatctttt ccgcgttttc ggtgaatcag ctggacgatc tgttgttcca
    50041 ggtcgggctg gggagaattt ccgccaaacg ggtcgcccat tatttcatcg ccggcgaaga
    50101 agtcgaagcg gcagaacccg cccgggaaaa aattaaaaag aaaaaagcgg catcgccttc
    50161 catgggcgtt tcgctgatcg gcattgacga ccctgacgac atcatggtga attttgccaa
    50221 atgctgcaat ccgattcccg gcgatgatat ttccggttac atctcgcgcg gccgcggcgt
    50281 gatcgtccat accagcaatt gcccctatat taaacgaatg gatcaggagc ggatcgtcga
    50341 cgtgcagtgg agttcccgcg aagagcacac ctatcccgtg aacatcaaag tcgtctgtga
    50401 cgatattaaa ggcatcctga cggaagtcag ctccatcatt tcatcgttcg acgtcaatat
    50461 cagctatgcc gaaatcgaaa ccgtcaacat gatcgccacc tgtaaattta tcatcgacat
    50521 cagcaatctg ccgcagttga accaaatcat gtccgccatc aggcagttga agtttgtcaa
    50581 ggccgtggaa tggctgcatc atttttaaat catattgaca aaaaaaatca ctctgttata
    50641 aacaggcccg actaaaaaaa agagagaaga cgatgtttca tacaaagaaa attctagtgc
    50701 tcatcacgtt tgccgttctg gcctgttttg gctggggcgc tgcggagctt attgccgcgg
    50761 agaaaaaata catcattctg atcgatccgg cccatggcgg aaaagatcgg gggctgaaac
    50821 tcaatgacga cgtttccgaa aaagatgtta ccctggcggt ggcgctgcag atcaaaaagg
    50881 aacttgcgca ggaacccaac ctccaaatca tcctgacccg ggagacggac aaaaccctgg
    50941 atcgggaagc cagacaaaaa atgatcaaaa ccctcaaccc ggacatggtg ctgaatcttc
    51001 acgtcaacgg cggattcggc aaggaagcct ccggatatga aatctattat ccgtcctgcg
    51061 gcgccgacga tccggcctca atgcggaaga ccgacaaaac caaaccgcaa agccagtgtc
    51121 agaacaattc tttaaaaatg gccaagctga tttcggataa tttcaacgcg ctctttccgc
    51181 gcaaggccag aggcctgcgc atggccgatt tgtccgtcac cgagggggtg ctggtgccgg
    51241 ccgtggcggt ggaaatgggc tttgccacaa atgccgttga ccagaaaaat ctgatgtcca
    51301 aaaaaacgca ggctgaaatg ggcaaggcgc tggccgtcag catcaaagca ttttccaggt
    51361 gagttgtgac cgaaagaaaa tcaggccgcc ggttcgatga actgaggccg ataaaactga
    51421 tcaagaatta tctcaatacc gccgccggtt ccgtgatggt ggaattcggc aacacgaaag
    51481 tgctctgcgc cgcgtcgatc gacgaaaaaa cggcgccctt tctgaggaat acgggccggg
    51541 gctgggtgac cgcggaatat tccatgctgc cgatgtccac gcagacgcgc acagcaaggg
    51601 aatccgtccg gggaaaaatc cagggacgca cccaggaaat tcagcgtatg atcggccgat
    51661 cgctgcgcgc cgtgacggac ctgagcgcct tcggcgaaaa aacgatttat ctggattgcg
    51721 atgtcattca ggcggacgga ggaacccgga cagcgtcggt cacgggcgcg tttgtcgcct
    51781 tggtgggcct gtttgatcaa ttgaaacgag acggcgtggt gcagaacatt cccgtgcgcg
    51841 attatctgtc ggccgtcagt gtcgggatca tcgatcagca ggtgatgttg gatctggaat
    51901 atgaagaaga ttcccgcgcg gacgtggata tgaattttgt catgaccggt tcggggcatt
    51961 tcatcgaagt gcagggcact gcggagaaaa tcccgttcag ccgggaacaa atgacacaga
    52021 tgacagacgt ggccgagcgc ggcatccggc agatcatcga acggcagaaa gaacttgtgg
    52081 gtgacctgat cggatgaaaa tcgtttttgc ttccggcaat cttggcaagg tccgggaaat
    52141 ccgggaaatg ctttcctgca tcaacatcga gcttgtctcc ctgaaagatt atcccgatat
    52201 cccggaaatc gtcgaagacg gaaaaacctt cctggaaaac gccctgaaaa aagccagaac
    52261 cgtttccgaa cggacagcac aggccgttct ggccgacgat tcgggactgc aggtggacgc
    52321 gctgggcggc gagccgggaa tttattcggc gcgttatgcc ggagaagggg cctcggacga
    52381 cgacaacatc aatctgcttt tggaaaaact caaagaggtt ccgccggaaa aaagaaccgc
    52441 ttccttttgc tgcgttcttg tattatacaa caccgacggc acctacgaat cttttgaatc
    52501 ccggtggccg ggccggatca ttgacgaccg ccgcggcgac aacggtttcg gctacgaccc
    52561 ggttttttac gtgccggaac tgaacaaaac cgccgcggaa ctgccgccgg aaatcaaaaa
    52621 cagaatcagt caccggggac aatccttctt gaaactgaaa cagtccctcg gacggaaaat
    52681 gaaaaaataa aaccggggcg tagcgcagcc tggtagcgcg cctgctttgg gagcaggatg
    52741 tcgagtgttc aaatcactcc gccccgacca ttcaaaactc cggcggtgta aaatcttttt
    52801 taatttattt tgcacagtcc gctcgggcaa cgcctgcggc cggccgttgc atcatcctga
    52861 aaaatgaaac gttgcaaaac gcatatcatg tcgcatggaa aagcgtaaaa ataattgatt
    52921 ggaaaataat ccaatatgga ttaagatacg cgccatattc ataaaaactt tttgaaaagc
    52981 tgtttcacga aagctcaaaa acatggggga gggtttgcaa tggagcgttt cagaaaatta
    53041 tgcgtgatca ttttaagttt gagttttatt ttcagtgttg ccgcctgcga taatttttca
    53101 aaattcaagg aaaaaatgtt aagcgaggag atcatctcct ttcccgtgaa gcagggaaac
    53161 agctggggct ttatcgacac gacgggcaaa ttcgtcatcc aaccccagtt tgacggagcg
    53221 gggcgttttt ccgacggatt cgccgccgtg aaaatcggcg accggtgggg attcatcgac
    53281 aagaccgggg aatacgccat caatccgcag tttgatgtcg cttttctgtt ccatgacgga
    53341 ctggcatccg tcggcctggg cggcggcaag gtcaaggtcg gattcatcaa caaacagggc
    53401 aagtatgtca tcaatccgca atttgatgat gccgggtggt tttcagaagg ccttactgcg
    53461 gtgggaaaaa tcgacgggca gaaagtcaag gccaaggtcg ggttcatcga caaaagcggg
    53521 aaatacatca tcaagccccg gtttgaagat gcgggctggt tttcggaagg cctcgcggca
    53581 gtcaaaatcg acggcaaata cggctatatc gacaaaaccg gcaaattcat catcgaaccg
    53641 cagtatgatc ttgctttttg gttttccgaa ggaatggcta cggtcaaaaa cggaaataaa
    53701 tacggctata tcgacaaaac cggcaaattc atcatcgaac cgcagtatga tttcgctttc
    53761 tggttttccg aaggcctggc ctcggtgaag agcaagggga agtacggttt catcgacaaa
    53821 agcggccggt acgtcatcga gccgcagttc gatatcgcgt tttggtatga aaacggtttg
    53881 gcgccggtcg gcatcggacg cggcaaggtc aaggtcgggt tcatcaacaa acagggcaag
    53941 tacgccatca atccccagtt tgacggtgtg aatgttttct aaagtcgggg agaaaaacta
    54001 aaaaaagaaa gggattgctt ccgcacacag atcgaattgt ttgctttgtc gccggcgata
    54061 acgccggcgg cgaggcgcga gcgctctttc catgcggccc ccataaccat gtccggcaca
    54121 ctctatccgt ttaaagtcaa tgacagcctc tggatactgg gcaacggcta ttttcatctt
    54181 tatctgctga agggcgccaa acaatgcgcg ctggtggaga ccggcatatc cgcgacggcg
    54241 gacctggtgc tttcgcagct tgaggcgctg gatgtccggc cggattatct tgtcgttaca
    54301 catccgcaca gcgatcacat caccggactg gaccatctgc gcggggcgtt tcccggcgct
    54361 gttgttatga ttgcggcggg cgccgcatcg tttgtcgcgc atccgaaagc cgcgccggca
    54421 attattgccg aagaccggca catgttccgg gcgctggcgg cccgcggcct gttcagccaa
    54481 accgccgtcg tcacaatgcc gccttcgctt tccggatgca gaatccttac cgacggggaa
    54541 gcccttgatc tgggcgggat gtccgtgaac tttctttctg tcaaaggcca ttccccggga
    54601 aacctgctcg cctggattgc cggcacaaaa acccttctgg tatcggacag cctgggcaac
    54661 cattatcccg gacgcggctt tttcccgacg ttttttaccg gttataacga ttacatggca
    54721 accatcgatc gtctggagag cttccggccg aaaagaatgg ggcttgcgca caacggattt
    54781 ttttccacat ccggagcgat tgcagacata ttttgtttat cgcgaaagtc cgcacaggat
    54841 gtccgccgat acatcgagga ttgcgcgatg gatgatgaag acctcgcccg cagccttttc
    54901 ggattttact atacggacga actggccgtt tattctccgg acaatatttt aaactgctgc
    54961 cgattgctgg ttcgccgtgt cagagaatta cacacggcgt agccggccca tccgccatag
    55021 attattttga gtcgacaccc gttattcggt tcagccgccg ctacacatgg agcagcgcct
    55081 ccacctctcc gcgaattttg ttgagcgtct ttacagaggc ggcctcgaag cgcagaacaa
    55141 tcaccggctg cgtattggaa gagcgcacca gcgcccagcc gttttcaaac ggaatccgga
    55201 tgccgtcgat gtccacaatg ccgggcgtat ttttgaaatg ctgtttgatg ccctcgacca
    55261 cctttgcttt tttgctgtcc gtgcaatcca ctctgatttc cggcgtggcg aatgttttcg
    55321 gcacatcggc aaagagaccg ctgagttttt tgcccgtccg ggacagaatt tccagcaggc
    55381 gcagcgcggc atagatcccg tcgtcgtatc cgaaataacg gtcggcgaaa aagaaatggc
    55441 cgctcatctc tccgcccagc accgctttga cctctttcat tttcgccttg atcagagaat
    55501 gtcccgcctt ccacatgatg gcctttccgg aatgcttttc gatatcgtca aaaagaacct
    55561 gtgaacactt gacctctccg ataatcgcag cgcggggatg attcttcaga atatgccggg
    55621 cgaacagcag cagcagtttg tcgccccaga taatctcgcc cttgtcgttg acgacgccca
    55681 gacgatccgc gtcgccgtcg aacgaaatgc ccagatccgc cttgtttttg cgcaccagcc
    55741 ggatcagatc ggtcaggttt tcctccaccg tcggatccgg aaaatgattg ggaaacgttc
    55801 cgtccggttc gcagtgtacg ggaatcactt cgcagccgaa ccgtttgagc agcggcacag
    55861 cgaaatatcc gccgacgccg tttccgccgt ccaccaccac cttgatcctt cgttgaattt
    55921 taatgcgttt gaaaagataa ttttcgtact ctttggcaat atccttttcc cggcagcgcc
    55981 cggcgccttt cgcatagcgg ccgctttcca tgattttgcg caggtcctgg atctgcggcc
    56041 cgaaaatcgt gctgtggccg atgcagattt tgaagccgtt gaattccggc ggattatggc
    56101 tgcccgtgat catcacgccg ccgccgacgc gcaggtgacg gatggaaaaa taaagcatgg
    56161 gcgtggcgca catgccgata tcgatcacat gaatgccggc ggcgttgagg ccgcggctca
    56221 gaaaccgatg atagctctcg gagctcaggc ggcagtcgcg tccgacggtc atgttcctga
    56281 ttcttttcgc cctggcgtac gtgccgatgg cgcgtcccag atggaacaca aaatcttcgt
    56341 ttaagtcctt atcgacaaca ccgcgcacat cgtattctcg gaaaacatgg ggattcatga
    56401 aacggtcaac tcctttggta aattataatt tgaatttcag cgtcagctcg taaaccccgc
    56461 tgtcaatttt cttcttgata tcgaaaccgc ccttttcaaa tacatgcagc atcggcttgt
    56521 tctcgacgag gacttccgcg gtgaacccga gcaagccctg ccgtttggcc agatacgtca
    56581 ggtatttgag caactcgaat ccgatgccgt tgttctgctc gctgtcgcgc acagcgaaag
    56641 cgacttcggc ggtatgcgtg gcgtcgtcga ttccgtattg gccgacggcg gagatatacg
    56701 aattatcgac atcgcctttg atcgccacca gcacaatctg tttggtgtaa tcgataatga
    56761 cgaaattctg cagccgctca tgcggcatat ccttgcgcgt cgaaataaaa cgccgttcga
    56821 tgcttttatc cgacagcgaa taaaagaaat ccttcaaaag actctcgtcg ctgattttaa
    56881 ccggtctgat gaaaacctgc aggccggtct tggtggtgag atacgtctcc aggtcctcgg
    56941 gatattcgcc gcgcttgccg ggaataaacg cctggtcctt gaaaatcagg ccgcgttttt
    57001 tcgcctcatc gatcagccac ggccggaatt tcggatgggc gatggcaatc agcgacatgg
    57061 cgcgttcgcg gatatttttg ccgtgcagat aggcgatgcc gtattcggtg accacatagc
    57121 gcacatcgcc gcggttcagc gtgaccccgg cctgttcctt gagcgccgga acgatgcggg
    57181 aaaccgtatc gttcttggac gtggatttca gcgccagaat ggatttgccg tttcgggcaa
    57241 acagcgcgcc ccgcataaaa tcctgatggc cgccgatacc gctgtaaaaa atgccgccga
    57301 tggattccga cgttgcctgg ccgctcagat cgatttccag ggcgctgttg atcgccacca
    57361 tgttgtcgat gcgggacatc atcaccggat tgttcgtata atccagcgtc cgcagcgata
    57421 tggaaggatt attatccaga aaggcgtacg tggatttgtt gcccatgcag aaagacgcaa
    57481 ccgttttgcc gtgatcgatg gtttttctgg aattattgat cacgccggct ttaattaaat
    57541 ccaccagacc gtcgctcaaa agttccgtgt gaacgcccag attttttttg ttggataaac
    57601 agaacatcac ggcgttgggc agactgcccc agccgacctg aatcgtatcg ccgtcctgca
    57661 ccagacgggc aacgtttttt ccgatggtta taatgacgtc gcctgtttta ggactggata
    57721 cttccagcag cggctcatcg taaggaataa taaaatcaac gtccctgatg tggatgaatc
    57781 cgtcgccgtg aatgcggggc atattgggat tgatttgagc gataacaatt tttgcctttt
    57841 ccgttgcggc tttgaccacg tccacactga cgccgaggct taagtaatga tgttcatccg
    57901 gcatggaggt ttgtatcaga gcaacgtcaa tctggacaac gccgctggct aaaagagagg
    57961 gaacctgcga aagggaaatg ggcgtatagt cggccatgcc ggcgttgatg gagctccgtg
    58021 tgctgttccc gatgaaaaaa gaattatggc ggaaattcgc tttgtatttg ggctcggtgt
    58081 aaggagcgag tcccagcgag taaacatgca taatttccgc gtcaaaaaaa gctttcgggt
    58141 gggattccac ataacggatc atcgccttga cgaggtattg aggttcgccg cagccggatg
    58201 cgacaaaaat atgatctccg cggcggatat ggctgaaaat ctcatcttcc gaaacgaatt
    58261 ttttgggata ggctttcttg aagccggcaa gatattcttc gaaaatttca ggttccatgg
    58321 cagtctgtag ccccggttta aattaaaaga aaaacgacgg tatcccgatg tttttcgagt
    58381 attataagtt aaaccgctgc ccctgtcaac cagccttcta gtgagatttg ctaagaacga
    58441 acgaagaaaa atcttgtttt tttgtatcca tcggcttcgc acaaaaatgt gaaaggtgaa
    58501 aagtgaatag tgaagaaaaa taagacttct cacatacgtt cgaagtgaca cagtccgcaa
    58561 ccgttgaacc ctgaaccttt tttcaccatc aactatgaac tatgaactat caactatacc
    58621 ccggaggtca tcccgttatt tcagaaactc cgccatgttc gtatgctttt caatcaattg
    58681 cgataaaaga ctgatcttgc cggtgtcctg ctttctgagc aggggttcca tatcgtcaat
    58741 cgtcctggcg gtctggaacg tgtccgttcc cacaagcaga acaggaatgc cccgttcgtt
    58801 ggccctggaa atgatgttct gatgcggaac aatgtcattc gtcaggatga tgcccgcggt
    58861 atctctttca agcgcggcaa gaatcagatc gcttcggtcg ccgccggtaa tcatcagttg
    58921 gccggtgccg ctgagttccg gacgggcggg aatctgttct ttgtcggcaa caaacgtcgc
    58981 agaggttcca atgagaatcg tcctggcgat atttttcaaa tgctcttcgc ccgcgagcac
    59041 tctggccaga agcttgtcgg cgagaaaatc aagcggaaag tacgttaagg gctcttcata
    59101 gggaatcgcg ccgatcacat cgaggcccat ctcccggatg accggtgtgc aggacgcttc
    59161 aaactcttca atgtcgctga tcttgttgat aataacgccg ccgaaatgga cgccttccag
    59221 tttgacatat ttttcaatga acctcatatc gtcgagcacg caatcatcat cgccgctcac
    59281 gacaatcacg agtacgccgt caatggactt ggtcatggac accgcatcca gattgagata
    59341 agcgccggcg gtcagatctt ttccgctttc gatgaagagc acgtcgttct cggcgccgat
    59401 atccgtcaca atctcggcaa gggctctttc gatgccttct ttatcataca tatatttcag
    59461 ccgggaatga tcgaacccga gcgtgatttt ttcgcagtgg ctttccattt ctccctggcg
    59521 tccgagaaga ttgaccatca ggctcgcatc gtaatcccag cttctttttt tgcgatagat
    59581 cagcctgtcg cccagcggct tggcgtaagc gaatttttta tcgtgcatca gcgagacgat
    59641 tcccgcgatg atgcttgttt ttcccgaatt gcgtctcatt gaagaaacaa cgatcttttt
    59701 cattgcgtat cctccttcat tctgcttatc aaaatccgcg cgtccacagc cttgatgccg
    59761 cttttggctt catagacctc gatgggattg acttcaatat cagtgatccg ttcacacgtc
    59821 ctgataatcg aaccgatttt aatgatcgta tcgatgacag attcaatatc cttcttctct
    59881 tctccccggg ctccgagaag cagcggataa gatctgattt cttccagcat ggacagagcg
    59941 atgctgcgat tgatgggaac cgcccgaaac gaaatgtcct tcataatttc cacgtaaatg
    60001 cctccgagcc cgcacataat gaccggtccg aaggagggat ctcttctcgc gccgataatc
    60061 agctcgatgc ctttcttgac catttccgcg acttcaatac cctcgataac ggcgttgggt
    60121 ttatacatcc tgcagtgatg catgatcgct tcataaccga cgatcacttc cttttcatca
    60181 tccagatcaa gcacaacgcc gcccgcatcg ctcttatgca gaatatcttt ggagaccacc
    60241 ttcaggacga ccggataacc gatttctccg gcaaactgca cggcttcctg aatgctgcgc
    60301 gctattttac tttgcggcat cgcaatatcc gcggcgcgca aaaccgccgc gccttcattg
    60361 gccagcagaa aggttcttcc cgctccggcg gcccggtcga taacggcatt gatcgcggcg
    60421 gtgtcgacag catactcgtc aattttgcgg gaaacctcgt caagatagtt gtgataccgg
    60481 tagaaagcgc ccatgcacga gacggcctgt tccacatcgt tgaatacggg aatacgctcc
    60541 gcgatcaaag cggtgatcgc gttttccacc tgtttgccgc cgatcagggc gtaactgatc
    60601 ggtttgccgt ttttctgatg gcgggcgtag gtctcctgga tcatttgcgc cagatttccg
    60661 gaatcgaaag tcgccgtttc gcaataaagc ccgatggtcg aaccgatatc ttcagattcg
    60721 gcggttacgc tgaacgcgcg tgtatattcg gttccgccgg cggcgccggt aagatcgatg
    60781 gggtttttcg ttgatccgaa cgatggcgtg acggcctcgt aaagccctct aagcgcggcc
    60841 tgatcatcat acagagagac gccgtacttt tcacaggcgt cggttgccat caccccgacg
    60901 ccgcccccgt tggtcacgat gacggtgttg cgggtccggg gcgccgaacc ggaaatcata
    60961 aacttgcacc agttaaacgc atcctcgagg cattccgccc ggagcacgcc gcattgtttc
    61021 atgatggcgt cgaatatgtc atccgagccc gccagcgatc ccgtatgcga agccgcagcc
    61081 tgcgcgcctc tcttcgattt gccggatttg ataacgatca ccggcttctt tccggtggca
    61141 accttgaggg cttcaatcag ccgttctcct tttttaacgc cctcgatata gataaagatg
    61201 acttttgtat cgtcgttttg aatcagatag gccagcagat cggcttcatc cacgtcgcac
    61261 ttgttgccga tggaaataat ggcggaaagg cccaggttgg cgacggctgt tttcgaaatc
    61321 atggcgattc ccagcgcgcc gctttgcgtc aggatggcga catgccccgg actaatcttt
    61381 gctgcggaga acgtcgagtt cagcgacgcg gcacgggagt acaggccgaa catattcggc
    61441 cccacgatac gcgttcccgc ctgcgacgcg atatcaaata tctctttctc ctcatggaca
    61501 tttccgactt ctgaaaatcc cgacgtgatg atctgcagga acttgacctt tttcagggcg
    61561 cattttttta ccgcatcctt gaccagatcg gcgggaatca ctatagacgc gcagtcgatc
    61621 tcctcgtcaa tgtccagaat atcggaaaaa atcttatgcc cgtcaacatc accgcctttg
    61681 ggactgacgg ggaaaacatt tccctggtat ccgccggaaa caatattgtt gaaaacggca
    61741 tagccgattt tctttttgtt ctgggaagcg ccgatcacgg cgatactttt cggctcaaac
    61801 agatattgga tatcgtacgg ggtggtgttg tccatatcgc tctccggcat atggattgaa
    61861 aaaatggtta acgtttgccg actgccgaca aaaatgattc taatgaaatg agccggatat
    61921 tgcaatgcct aatataaagc ccctgcacgg caactccact gaaatgtgca ggacgaacta
    61981 tccaatgggt ttgccattgc aaattatccc cgaagtgtag cggatgttga cggcggcacc
    62041 atggtccgcc aggctccctc cggaatggat ccaaagtttg tcatgccggt gcagaccggc
    62101 atccataacc ctgtcggtca taccggcgaa ggccggtatc cggtttgtct cttttttcgt
    62161 catgtcggtg cagaccggca tccagtattc attcctggtt cccggctttc gccgggaaga
    62221 cgaggaggtg actcatattt catacctgga ttcccgccag gggcacgcgg gaatgacagn
    62281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    62341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgggggcaca gcggggttgt
    62401 gtcacttcga acaaccatca accatcaaca atgaactgtt tctttaacct ttaaccttga
    62461 accttggact ttttttaagc gcgaagcgca tcggggcaca gcggtgtttt gcctaaaacg
    62521 taaaacctaa aacctaaaac ctaaaaccta aaacttaaaa cttaaaacta ttattttctt
    62581 taaccttgaa cctcttttta ccatcaaccg tcagctattt tcaaaatcga caaaatctct
    62641 gccgcgttac ccagtaccag atccgcgccg gcggcgacga aattttcttt cggacaccgg
    62701 ccggtgagca cgccgcaggt cagcgtccgg gcgtttcgtc ccgtcgtgat gtcgatggga
    62761 tgatccccga ccatcagcgt gttgtcgggc agggcgccga gtctgtccag cgccagacgg
    62821 atgtgttccg gatgcggttt gaccctgcgc acatcgtcgc ggcagaccac cacggggcag
    62881 taggtcagaa tatccgggaa aaccatgttg accgcgcgga cgcagtttcg ggtgataatc
    62941 ccgcaggaaa tttttgccgc ctgaagcgat tccagtagat cttttgtcca gccgaacaga
    63001 tggccgtttt gcgccccctg tgtttcgata tcttcaatca tccggttggc ccgtgaaaaa
    63061 aacgcgtccg cctgctgcgc cgattgccgc cggatcaggt catgggcggc atcgatcgtt
    63121 tccagaacaa aggttgtctt cagctcttga ggtataatcc ggtacgacgc aaccagcgcc
    63181 tcgatgtccg agcgcatttt ggaaaagtcg atggtcagct ttgccagcgt gccgtcgaaa
    63241 tcgaaaatga ccgctttgat cgttgtctgt ctgattttca gcgatgccat aacatcagcc
    63301 ccggggagaa atataacggt tggtcagcgt cttggtcatc attcgggcgg acgtcgcctc
    63361 cggaacagtg cctccggaga tcacctgaag aaaattgcgg atgatattga aggtcgctcc
    63421 ccagagaata tcgttattgc cgccggcatc gggaatcacc aggcagggcg tctgatagca
    63481 aaacggctcg ttgctttcac ccacggtcat gtggacgtcg agcgtggcgt agttttcgct
    63541 ctcgaagaaa aagcttaaag ggatttccag caccttttcc acttcggaac tcagcctgta
    63601 tgaatacggg cggcggatgc ggcataccaa cgggaaaatc gtccgggaca gaaacgtcag
    63661 agagtaggag ggcagggcgc ccaggagttt tgcgttcagc ggattcagac cgatttcctc
    63721 ccaggcttcg cgcagcgcgg tcgtcagaaa aagccggatg agacggtcgg tgtccgcatc
    63781 ggcatgctga atgtgtccgg taattctgaa gcccagcgcc ggcagcattt gccttttgag
    63841 aaaagaactc agcatattat cctgacgggg ttgcagcatc ccgccgggac aactgatgtc
    63901 gccgccctgc gaaatacttt ccgagcgctt gatcaactga aaaacatatt cggagcggcc
    63961 gttgaattct ttgaaataca aaggcagaac caccgccgcc ttgagatgag cggacccctg
    64021 tttgcgggat gcatcgatct cggcatcttt ttccttgaaa tctaccggca gcgccgccag
    64081 tttttcgacg atcaggcggc tcaacgcaac tttattctga aaaaggttca agtccatagg
    64141 ctgtgaaaac tacgaaacga tagcggcaat ggccttctga taccagcacg acgcagggaa
    64201 caaatccaga aacccgcagt gcccgccgta ccgctgtttt aaaattttaa gattgtgatt
    64261 tgcatccata ttgagaaaat cctgataaga aacaatcgga tcgtcttcgg caatcagcac
    64321 cgtgagcggc agcgacagat atttaaacgc gttatatttg agcgtgtaca tcttgaaata
    64381 gtgctggtag ctctggatct cgggaaaata cgggatcagc attttcgtca gttccatgca
    64441 gctctccacg tccatcagat cgctgaaatc gtaccggtcc ggaaaaagtt tttgtttttt
    64501 catcagggac cgcttccatt ttttcagaaa ataataccgg tagaaggcgc ggccgttatc
    64561 gatggccatg gttgctttgt agggattcat cggcgggctg atggcgaaca cgtgttcgag
    64621 atgattgatc ggtttcatac cgtggcgcag ggcgatgcgc attgcgaaat tgccgcccag
    64681 cgaaaacccg acaagataaa ccggcttgat tctggaaaat gaggccagat aatccacccc
    64741 gtcgaacgtt tctttcaggc gggcggcgtg gaagagatct tcgttaagat gatgcgaatc
    64801 gccgtggtcg cgcagattca ggcggccgat catatagccc cggcggtaaa aataatcggc
    64861 ggaatccaga atatatatgg aagacgacga accttcccag ccgtgtagga aaacgaacat
    64921 gcctttcggc tcgggatggc gggtgagata agacaccagc cgggaaccgg tggacgtttt
    64981 taaaataacc tcctcggtgt ttcttgccag ctcgttgcgc cagtgatagt gcaaccggag
    65041 acgggaactg gccatgatcg actggacatg cggattgcgc aaaaacaaag cgggctcaaa
    65101 cggatcatcc gtgatgttgg tcttttcatc cataagcagt gataaaacat tgaatcctga
    65161 ttcataaaat caaaaaattg gaatttaagc aagcgcataa tttccccggc ccgcttgccg
    65221 gaagccgtcg cgccgcgggc cataaacatt gaactcactg ttccccatca gctgcgctcc
    65281 ttaaaaagtt caaggttcaa ggttcaaagt tcaaagttca aagttaaaaa cactgttgtc
    65341 cccattcgct ttccttcccc tgtcattcc
//
LOCUS       DAOSME010000005        62623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_606_length_62623_cov_22.853392, whole genome shotgun sequence.
ACCESSION   DAOSME010000005 DAOSME010000000
VERSION     DAOSME010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 62623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62623
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_606_length_62623_cov_22.853392"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLG98_01350"
     CDS             complement(<1..331)
                     /locus_tag="PLG98_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31925.1"
                     /translation="MKTKTVSPFIQKTSVKDWADWRWQIKNRLTHPLLPCAQPQTAAS
                     HRETLQSVTAVYPMAVTPYYLSLIQHQDFHDPIYAQCMPDPREISTAGRAAEDPLQED
                     LHMPVPKL"
     gene            482..1465
                     /locus_tag="PLG98_01355"
     CDS             482..1465
                     /locus_tag="PLG98_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOE31926.1"
                     /translation="MQQRKENVAMLSVASNAFLVLFKVLVGFAIGSVSIISEAIHSGV
                     DLLAAVIAMFSVKTSSVPADERHPFGHGKIENLSGFVEALLIFGAAFWIIFEALKKLN
                     APQAMENVGWGVGVMLFSAVMNFIVSQKLFKVGKEADSIALQADAWHLRTDVYTSAGV
                     MFGLTIIWLGHKFFADPNIYWVDPVAAILVAALIMRVAYRLTVDALSGLVDVQLPREE
                     ENFIRSMISVEKAVYGFHQLRTRKAGHVRFVEFHIKVDPQMSVHDSHGITRILKKNIR
                     ARFPESTITIHIEPCDGECSDMCQAGCLLPREKRPDKGMIRPQPIGHDQSS"
     gene            complement(1477..2259)
                     /gene="truA"
                     /locus_tag="PLG98_01360"
     CDS             complement(1477..2259)
                     /gene="truA"
                     /locus_tag="PLG98_01360"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOE31927.1"
                     /translation="MASYKFKILLEYDGTGYRGWQTQKNARSVQETLIKAAQSFLDVP
                     VRIQGAGRTDAGVHALEQAAHLESPKKVHPDILRTGMNDHLPSSVNILRVENAPARFH
                     ARHHALSRSYLYLISRRRTAFGKRYVWWVKDRLDCDKMKQTLDVFQGFHDFASFADKR
                     MDKEASTKVNIEGLWLKEFDNIIAVRILGSHFLWKMVRRIVGVTVELGRGNLTRPDVE
                     KMMTSFSELPAHFTAPPSGLFLEKVLYEGDKLPPVTPPICLL"
     gene            complement(2341..3681)
                     /locus_tag="PLG98_01365"
     CDS             complement(2341..3681)
                     /locus_tag="PLG98_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HOE31928.1"
                     /translation="MKVKYVCVVLALFLVSCYLQPPDTLQDVKDVTDLPQDHTARLAD
                     VPADKVLLTRDSQTAMNEHYDKTFFSVWHHPEPVHALPERLSAIFTQFAAHPGYGENK
                     RKHSTAWLKKLQQNAWLENYPNTLQPAITVRSTNLRMLPTNGPHFYKPGGDINGWPFD
                     NLQISSVAANTPILICHISRDRSWALVETSFAFGWIPAEDFARVDDPFMQTWQTSRYA
                     TVTRDKIPIVDANGNFSLRTSIGQMFPLITEMADKLEILIASADANHQAIIQRVFISR
                     EIMNIKPLPFTYANATRIANELIDEPYGWGGLYGNRDCSAMTRDFFAPFGIWLPRHSA
                     DQAKKIGIYIDLEAMEPQEKEKVILEQGIPYLSLLWRRGHIMLYIGERNGRALIFHNV
                     WGLRITDARGEEGRKIIGKAVITTLHPGKELTNIDPDALLIKNLSAMTILAPAK"
     gene            3891..4439
                     /locus_tag="PLG98_01370"
     CDS             3891..4439
                     /locus_tag="PLG98_01370"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOE31929.1"
                     /translation="MDSFVPKKIFFTKGVGTHKDELHSFERALRDAGIEKCNLVQVSS
                     IFPPRCKMISKTQGLKQLTPGAITYCVMSRCCTNEPKRLMAASVGCAIPADKSSYGYI
                     SEHHAFGQNEKQAGDYAEDLAAAMLASTLGIDFNVDESWDEKKEIFKISGKIVRTLNV
                     TQSTICKDNKYTTVIAAAVFVF"
     gene            4526..5404
                     /locus_tag="PLG98_01375"
     CDS             4526..5404
                     /locus_tag="PLG98_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE31930.1"
                     /translation="MTQTERILDIPSLNHHDIGGAHLPYLYYEGETPQILFAHATGFL
                     PWLWHPILEELTPPRRQAWAPYICNYRDCDPESGGLSWDVIARDLISFCSVQNISDNL
                     AVGHSMGATVVTIASALFGLQPRGMILIEPIFLPEDFYTIDIQLKDHPLASLSIRRKN
                     GWKDENAALEYLKSKSLFAGWDEQMLQFYLQYGMEKQAGGDLKLTCAPRNEAALFMGG
                     KSTNPWPLLEKLTCPVLILEGEKSSNREFIDLPKAASLLRDGRYQLIPDAGHLIPMEK
                     PKEIAKIIKEFLADVF"
     gene            5546..6331
                     /locus_tag="PLG98_01380"
     CDS             5546..6331
                     /locus_tag="PLG98_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE31931.1"
                     /translation="MSKPFALIGRNVIRWINSIGAATIFLVLALLKTFHPKQFQKVIQ
                     QIYYIGARSTTIIMLVSLFTGMVLGLQSYHALVQFGAEGSLGTLVSLSLIRELGPVLT
                     AIMITARAGSAITAEIGVQRISEQIDALETMRIDPFRYLVTPRITASIVSFPLLTTVF
                     DLIGIIGGYVSAVALMGLNAGVYSHSIRSYVDMEDLTNGFVKSLVFAVIVATVCCYQG
                     YFVHMREDSKGAKAVGLATTSAVVISCVLILVADYVVTSLLMV"
     gene            6339..7583
                     /locus_tag="PLG98_01385"
     CDS             6339..7583
                     /locus_tag="PLG98_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE31932.1"
                     /translation="MNTPLIEFKNITKRFGEQTVLDQVSLQIYEGEVTTIIGLSGSGK
                     SVLLKHIIGLLKPDEGTILFRGEPINDMKKTELAEAMSKISYMFQSNALFGSMTVYEN
                     IALPLLENTAMKKNEIHDRVMARIEQTELTEAAQKYPSELSGGMQKRAALARALITDP
                     QVVLFDEPTTGQDPVRKNAILSMIAQYQKKFNFTAVMVSHEIPDVYFISNRILALYDK
                     SIVFQGTPEELSNFDHPFNDEVIRSLEGLQKELTGLYSRRQFKVLHRSQLQSRKSDEG
                     YCVVVFDLSDMNSIVSAIGYDRAQETIHSMGKYIDKHFDAIGGFSTRRKINEFVTVLP
                     FSDIAETESIVKDFMEDFQKSGMSEIWSEAQKNDPQTRCVDFSVKAGMAEGTPVAEID
                     SIVKLAKAHRKEIGRLQCAVKE"
     gene            7588..8043
                     /gene="mlaD"
                     /locus_tag="PLG98_01390"
     CDS             7588..8043
                     /gene="mlaD"
                     /locus_tag="PLG98_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOE31933.1"
                     /translation="MKKYEIETVVGIFVFLGLLCIGYMTVKLGNVSFLEAASYPLEAK
                     FTSVNGLRIDSSVKVMGLTVGKVKQIAMDQDNQRAVVKLNIQKGIKIYDDAIASIKTE
                     GLMGDKYVSIDPGGGGSLLEPGGIILETQSPLDIETLISKYAFGDVKKQ"
     gene            8078..8674
                     /locus_tag="PLG98_01395"
     CDS             8078..8674
                     /locus_tag="PLG98_01395"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE31934.1"
                     /translation="MKKLFTQTAIILVIVMFSLPVYAGAALDSVQKCVNEVIGIVGDP
                     KLKSESAKELKKEKLRVLYKQMFDEIEFSKRTLTRNWNNFTPAQRTEFVNLFEQVLET
                     AYGDKVLSYTNEKVVFYKETTLSATQAEVQSKIVTSSKEIPVFYRVILKDGKWKVYDV
                     VVENVSLVQNYRTQFNDILSKDKPEKLLEVLRNKVKQH"
     gene            8742..9671
                     /locus_tag="PLG98_01400"
     CDS             8742..9671
                     /locus_tag="PLG98_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOE31935.1"
                     /translation="MKSRLVFLVSFIWMLSGGAPGSAHAFVDLPPEQTSPPPVVLTVS
                     PDRPANQFAAAAVVDPMEEEYTGDEFDDYAGDNAKEEKVSIADPLEPFNRAMHEFNDK
                     MYFWAVKPVAQGYKAVVPEPARISVDNFFSNLGFPARFLSCLLQADFKGAATEAGRFT
                     INTVWGIGGLLDPSSGKELNLQEQNTDLGQTLGVWGVGQGFYIVWPVFGPSTPRDTVS
                     IAGNYFLDPVAYLNPWSARLGVNVLDKVNAASLRIGDYEALKNAAIDPYVAFRDAYIQ
                     YRYKQIKARKAKIFSGSSAAEPFPETVRPDKVD"
     gene            9672..10409
                     /locus_tag="PLG98_01405"
     CDS             9672..10409
                     /locus_tag="PLG98_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31936.1"
                     /translation="MKKIRCAPYWMVFVLTALVFFTTGPAASAAGLFSAFETVSRQDG
                     GLNTAIGYRYHQDTYEKGNGLKIRQNEIYSQAAYGADNAWEIYARLGIADLKIGDVFR
                     ESEEVFATLGGKVFYPVSGKLGVGAFVQGTYYLNDFSDTVERRKHVQLKDLWDVHFGV
                     ALQATLPCGSTMYAGPYLYYSEGTLSLSSNVSDWGYAAGRYSVQNTSIAGGFAGLDIP
                     LAKGFRLNVEGQYADRFSAGAAISYTY"
     gene            10467..11183
                     /locus_tag="PLG98_01410"
     CDS             10467..11183
                     /locus_tag="PLG98_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE31937.1"
                     /translation="MNQQKEKFFQVQDLTKVYQMGEVAVHALRGVTMELYAGELVVLL
                     GPSGSGKSTLLNIMGGLDTATGGVVSYRGKELTKATEKELTEYRRFHVGFVFQFYNLI
                     SSLTARENVSVVTEIAPDPMAPQQALDLVGLSDRLNHFPAQLSGGEQQRVAIARAISK
                     KPAVLLCDEPTGALDSQTGIVVLQALERINRELGTATAIITHNVDIAGMADRVIHLSN
                     GHIASVEINKVKKSASELQW"
     gene            11185..13551
                     /locus_tag="PLG98_01415"
     CDS             11185..13551
                     /locus_tag="PLG98_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE31938.1"
                     /translation="MMKTLNRKLWRELWLMKGQVFAITLVVMSGVATFIMFISTMHSL
                     DFTRSKFYREYNFADVFVNLKRAPETLKYKINDIQGVHQVETRVCAQAKLDIRGFKES
                     VTARITSIPDDGDPLLNRLYIRKGRLPDPARDNEVAMNETFAQAHGLNPGDKFAAVIN
                     GKWKRLTVTGIVLSPEFILLMKTGAMTPDFKRYGALWMTRKAISKAYDMEGAFNDVVL
                     TVYPGTALSDVVRDLDNILGRYGGFGAYGRKDQISHRLLSEEFRQLKTSSRIYPTIFI
                     CVAAFLLNIVMSRTISTQREQIGTLKAFGYNNLSIAVHYATMVMMIIATGVLLGIAGG
                     IWFGKMLGALYMETYRFPFFHYILHPQVMIAVVVISVAAALSGTMHSLWNAARQTPAE
                     AMRPEAPVKYRVSLIEKTGIGRWLSQPSRIIFRNIERKPVKTVLAIIGIATACATMIC
                     SGFFRDCIDYMVHIQFVLSQKEDMTVSFVEPTSYRAVYELKRMEGISSGEAFRSVPVR
                     MKFGHRSYKTVINGIQPDSHLNILLDNHLKSISMPPSGILLTDYLGEILGVKAGDLLT
                     VEALEGSRAVRQVPVVAMAKQYLGVMGYMNLEALNRLINEGDAISGAYLVTDETHREK
                     LFQLFVQMPRVAEIVVRQNEIRSIYEIMAKAMLFFMFIATVMACSIAFGVVYNSARIS
                     LAERSRELSSLRVLGYTRGEIAYILLGELGFITLVAIPLGFIIGYWICAYVAHALTTD
                     LYRIPMMIELKTYAQAALVVIVSAVISGCIVRHKLNNLDLVEVLKTRE"
     gene            13587..14783
                     /locus_tag="PLG98_01420"
     CDS             13587..14783
                     /locus_tag="PLG98_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE31939.1"
                     /translation="MNHVLRRKIFILSIMAVVAGLLIYGFLPKTRDVDIVFVTRGPLQ
                     VTIEEEGRTRLKDRFTISAPTAGYLQRIHAKVGDNVQQGQIVAVLEPLQSQALDPRSH
                     AAARAAVSAAEAALKAAVEREKVAAADAAYLDQRRERLKALYDKGSIARDQFDQIHAE
                     AQKARALQLSSAAEVHRAGSELEQARVALRNFSAVRSQGNAVAVASPVNGAVFRIYHE
                     SEGAVNIGAPLLDLGDPKNLEVRVEVLSSDAVKIRQGTHVLFKRWGNDEPLTGSVRLV
                     EPAGFTKISSLGVEEQRVLVIVDITSPPEQWSVLGDGFRMEAHFTIWEDDNVLQIPAS
                     ALFRAGKDWAVFVEHDGKARKRVIEIGQRNGLTAQIISGLKENEKVIVYPDDTISEGT
                     KIRQRK"
     gene            14810..15346
                     /locus_tag="PLG98_01425"
     CDS             14810..15346
                     /locus_tag="PLG98_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE31940.1"
                     /translation="MKVFIIVGMPAAGKNIARIYAGSRDIVYYSTGDIVRAEVKARGL
                     EVDAATTSEVSDELRGEDGLGVTRLALEEILKTGADVGFLEGMRSWQEIELIREKTDG
                     VVVAVLTPRGLRLERVCSRGRDDDSPLGFNERDQREIAYGTAIPIALADAYILNTGTM
                     DDAMEQLEAIVQREMTPS"
     gene            complement(15782..16522)
                     /locus_tag="PLG98_01430"
     CDS             complement(15782..16522)
                     /locus_tag="PLG98_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE31941.1"
                     /translation="MKITFLGTNGWYDTDCGNTICTLIETKTAYILLDAGNGIYKADK
                     YIKKNLPVYLLLSHFHIDHMEGLHILAKFRFTSLEIIGQTGTKKTIGEFLSPKYSLPV
                     TRLPYPCNVTDINEGWHRRPFRFRALPLEHASRCFGYRLEMEGKVISYCTDTGYCKNS
                     EELSVNADLLISECAFLSGQQSKTWPHMNPQLAARLAVKSQAKRLALTHFDAALYTTR
                     AKRAKAQMQAKRIFPHTFAASDGLSIVI"
     gene            16732..18816
                     /locus_tag="PLG98_01435"
     CDS             16732..18816
                     /locus_tag="PLG98_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOE31942.1"
                     /translation="MTNDIITLNEFDGTALLKKGGIPVMDSILARDDKEIDVAANQLG
                     FPVALKICSEKILHKSDRGGVALNIADTSSLADAFRDMNNRFSDVPHAFLVQKMARPG
                     TELILGARRDPVFGPVILAGIGGIFTEIFRDTAIDLAPVDNTIACAMLGRLRGAALLD
                     GYRSQKALDVVAIAEALSSLSRLISDRPDILEIDVNPFIAYPDGAVAVDALVRLGGSH
                     ARSARKHPEPATVVPFFNPASIAIIGASRSPGKGGNIILRNLQKAGFGGAIYPINPTM
                     KEILGLPSYAKVCDVPAPVDLAMIVIPKTAVPEALEDCKAKRVTNIILSTGGYSDAGK
                     AGAEEQKIIIEQARKADIRIMGPNSIGTLNPSAGMATSIVGLEPIKAGGVSIIGQSGV
                     FSSGWARWIADTKPFGLAKVACIGNKGDINESDLLEYLAEDWVTLTVGMYLEGVIDGR
                     RFIRAAEKASRKKPVVVMKAGRSEAGAAAVASHTGSLAGSDAVFDAVCRRTGLVRTHN
                     PEAFFDTLSAFEKLPLPRGNRMGVFSITGMGCVAATDAAEEYGIVLPELNPATLKKLA
                     EVIPAWAPVRNPIDTWSAIEQHGSKKTSIHIAQCMLDQRDMDALLIIFVLMPESMFDI
                     PEAFAELFRNHPDKPVFASYYGGTAREIAHVHEGFSRLGVPCYPTPERAMFAFGRMVE
                     YARHRGRIRTRP"
     gene            19236..20741
                     /locus_tag="PLG98_01440"
     CDS             19236..20741
                     /locus_tag="PLG98_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31943.1"
                     /translation="MKQVAWSKSRRPKSKPIFYKNLLIMMAIAVLGIAMVPVTGQAVS
                     RVPLNASGIYSATADDGSKIFLYRYAPYTTGTPKFNTSATPVLIFTGITMNMNQYLSC
                     TPPDKKDVYSSVYVPPVDQAPEWALNEDKTDYEPYIKADKMRYYSLAHYLWLKGYDPW
                     FVNYRGTGRGEVRSDGTNANSLTTLDTWATQDAPAAIAKVKSVTGKRMFIGGHSTGGL
                     VSYDYLQGAYMESGIASSAWAKKAYYKLCYSLGYMPHVRASADLAKKRNEDVKGFIGI
                     DPAGVPSLPNLLDTPLFWTIVGSRLYLPLDYLSDNLIQVLPSKPLVGLLEGMFGLLNK
                     AAAGDSAISDFFDYLNFWVVEDMDPCLEDWTVRYAVGSAAIRGFGHYMDMGLNNTLRE
                     HYLNGKENYLSSKLVKGVSAPNPGRDGYYYYSENMARMTVPMIVFSSSTGSLVSPQAT
                     YDFIISKKTPTAYDEWYVLDGTGHFDLAMGKKMPTEMFPRLGAWLDTVDAL"
     gene            20991..21560
                     /locus_tag="PLG98_01445"
     CDS             20991..21560
                     /locus_tag="PLG98_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOE31944.1"
                     /translation="MEQTFTITVNVQELFMEFIPKLAGQYIQMRGDQEELKGTELKIT
                     LDISGSVYSYIIQDGVGFSIREGAVDNPHVRIAFSPDTLSKMADMKNIDMLIAMQKQL
                     TRKKFDVLTALSGKGVFQIKNADDTVSEITAIFNGAEKPIAVLRLSMQDANMISGGKE
                     SPINLFMSGKMKIEGDIAFAMKLQPLFVA"
     gene            21633..22070
                     /locus_tag="PLG98_01450"
     CDS             21633..22070
                     /locus_tag="PLG98_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31945.1"
                     /translation="MPIKRIFFKTRLAFLMYGFACMMRLAVLISKDFRAHLATHDYSF
                     IMGSPEEDTYRYFQFIRGRVQSVRGRRPSDFALIWRDNRSGGKVMIDLAAGKRKALNR
                     AVVHGLLTLEGEGKYISLLIETMNRLNRLFRHERKTNNRVKAG"
     gene            22480..24300
                     /locus_tag="PLG98_01455"
     CDS             22480..24300
                     /locus_tag="PLG98_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOE31946.1"
                     /translation="MSEFKPYNDTTIPGIFRNQVEKSRDIIFLRIRDENKKWKEYTYG
                     DISGQVDALASYFLKKGIQPTDKIAIYANNRPEWAVADLATLAIGAADVTVYPTNSGP
                     EAEYILNDSRAKICMCAGKFQVDNLLKEKNNLHYLQEIIVFDEIDSRYPIVVKFSSVL
                     QIGKASLNLDEIDKRLRNIDVQETATLIYTSGTTGNPKGAMLTHSNLVSNVKQFVEHH
                     PFPPRGTCVSLLPLSHALERTVGYNSAMHEGCTISYTKGAETLLGDLKDVRPTLAVYV
                     PRVLEKIYEGVMSQLGNAPPSKQKLFHASMGVGKKALPYILYNKPVPFFLNLQYKLFE
                     KLIYSKLRKALGLDRMVTIGVGGAPLSNTINEFFQIIGVEIHLGYGLTETTPITHLNT
                     YKHLKPIKIGTCGPAFPRTECKIAEDGEILIRGPQVMKGYYNRPEDTAQVLTKDGWFY
                     TGDIGTIDEDGYLTITDRKKDILITAGGKNVSPQVIEGAFVMHPLIEQIAIMGDQKKY
                     LVALIVPCFTELIKWAKSKNINETDPTKLIAYPEVVQKYKETIDELNKPLGRVEQIKR
                     FSLINHAFSQESGELTPTLKIKRKIVLANYQDRIEEMYKE"
     gene            24414..24611
                     /locus_tag="PLG98_01460"
     CDS             24414..24611
                     /locus_tag="PLG98_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31947.1"
                     /translation="MHYRICKTVTIVLFTMLAMFCLSCTEAKCKIDQTVCNYDCPSTI
                     GVKQACEQKCNLLYDICRSQK"
     gene            24876..26390
                     /locus_tag="PLG98_01465"
     CDS             24876..26390
                     /locus_tag="PLG98_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOE31948.1"
                     /translation="MTTPIQQKIRDKKRTAKQIAEMVKSGDWINHGAVGGDSTACTEA
                     VAKRLGGGKSDLHDIEFWLAGNFYPHPELQEIDPMQQYHCVHEHFFFPWNRKAREDND
                     VTSWTPWGWAGGIWAHHYRFANGERKNRGIDWWFNAATAPDNHGYFNMSYGTNSSNIF
                     KQTAKKIVFEVRADYPWAKGCRFNTIHIDDIDYWVEVDCEKYKWPQMNEKGIVARPME
                     QEIASHIMTIMRDRDVVQLGIGSLPSACVAAMADAGFKYLGIHTEMLNYGLIKMIESG
                     QVTNAYKRLDQGKSVWTFAFPVDVKWYYETVHRNQALAVYDIDYTNNLDVLRRVDNMI
                     AINNTVAVNLLGQMTCSFYGKRPISGTGGFFQFIIFAAQSKGGRSVAAMTSCSKHNTP
                     RIVPFLPEGSVVDVPAQFAQYVCTEYGMVNLRGLNGYERARALISIAHPDDRAWLEKE
                     ARRHGLMPPQFPANFLPEEGGNRRYPSYSDRRSYKMPMGSSIWGYDWDPIQSGK"
     gene            26470..27312
                     /locus_tag="PLG98_01470"
     CDS             26470..27312
                     /locus_tag="PLG98_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31949.1"
                     /translation="MKVIPADASTVVLLRSCPDASVRGIEVLMVLRNRKSSFVPGYYV
                     FPGGGLENDDFADGMERFIVGMDRLAASKILSDMRYPEQALGAWVAGIRETFEEVGIL
                     LAQKKDGSPVTIRAENERQRFCDHRKALISGKMRFIEMLATEELLLPLDRLYYFSHWI
                     TPEPFPQRYDVRFFVTEIPSDQAVIHDGVELTDHVWVRPAEAIRQYEQGRLDMVLPQI
                     MTLENIKNCKTVAEVIARVRKRNVPAILTKIKRIDGKDVEVMPDGAVFENRPPAYSWP
                     DKSD"
     gene            complement(27377..27889)
                     /locus_tag="PLG98_01475"
     CDS             complement(27377..27889)
                     /locus_tag="PLG98_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31950.1"
                     /translation="MMQKTKWFMARFIFLTAAMGTALSGGLLGYVLCPLFSWYFFKDL
                     NFIKYHHYIIRLVFAFWRQVVELLYNPDYREMFYIPWTDPPINAPDPKRVRVRALWQH
                     SDKGCGLCNNCCTRRACPLHDMKHNQCKSYGSFFWRYFNCGRYPENTKQIHYYECKKW
                     ERYNCLSENE"
     gene            complement(27892..29736)
                     /locus_tag="PLG98_01480"
     CDS             complement(27892..29736)
                     /locus_tag="PLG98_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOE31951.1"
                     /translation="MKNPHLKILFLKWLPPKHVMKLTLVWFLQATAVIGYVNYTYDGY
                     FMRLFMHWIIVCLLLIVLMIPFDLKSFKIVPSVVRRITLVLILSFVHYFLLCFYVMTI
                     LGYKSWSGPFTFELLSAYSGHIDVLLNIYGLSVEAAIIIFLFIWLLIFAGYYYYSGVA
                     AEEFTANENDATQPHSRWPVLVLFLVMIFAIVYSNSYKSWQMSEPLHIAWLNGHGCLR
                     QAPQGIFNKRPLSGEKQSTTSAEPYEGITPRPLVLITVDALRSDQMGVYGAPRETTPF
                     LSHLWRGGKLRRIDNAYSICTTSFCGMIGILSSKYWHQLNEPPVNLADVLKKYNYRNS
                     FLLSGDTTNFFGLRQVLGSHIDLFCDGSIEPKKYANDDRSVLGWLQTYNWKSADRTFL
                     YIHLMSAHIIGLRDPRFKKWLPDKEWDIPKLFYSQRAYQNNYHNGVLQADNTIRQIFE
                     ILDREGMLQRALIVITADHGEFLGEFDNFGHGFEPFEPMVRIPMLVYDGLQSNYPERS
                     LSSQVDVAPTMLYAIGAAIPKDWSGIPFQLAAPRKAVYTACYDVSGVVADIGGQRFKF
                     LQKRKGGKEMLFDLDTQKAESINLAAKPDMRKILASMRELNQLTQKSK"
     gene            complement(29855..30649)
                     /gene="trpA"
                     /locus_tag="PLG98_01485"
     CDS             complement(29855..30649)
                     /gene="trpA"
                     /locus_tag="PLG98_01485"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOE31952.1"
                     /translation="MKPIENKFSELRARNEKALIVYLTAGDPSLDITKQLIFALEKSG
                     VDILEIGVPFSDPTADGPVIQAAAQRSLKAGTTLKGVLKMVADVRKTSDIPIVLFGYF
                     NPIFAYGVKNFAQAARHAGVNGVLVVDLPYEEAKELRDYTDAVGMDFISLIAPTTDKE
                     RLKKIASKASGFLYYISITGITGTAAPKIGNIKTEVSKIRKITKLPIAVGFGISRPEQ
                     AREIGRFADGVVIGSAVIRLIDENKNNGDLSKILSDYISNIKKELR"
     gene            complement(30649..31842)
                     /gene="trpB"
                     /locus_tag="PLG98_01490"
     CDS             complement(30649..31842)
                     /gene="trpB"
                     /locus_tag="PLG98_01490"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOE31953.1"
                     /translation="MKNTLPDKNGHFGIFGGRYAAETLMPALIELEKAYKIAQKDRGF
                     EKEFEWYQREYAGRVTPLYCARRMSESLRGAKIYLKREDLNHTGSHKINNTLGQALLA
                     RRMGKKKVIAETGAGQHGVATATVAALFGMECKIFMGEEDIRRQAPNVFRMKTLGAEV
                     VPVKSGTATLKDAMNEAMRYWVSSVRDTFYVIGTTAGPHPYPGMVRDFQSVIGKETKR
                     QIMKFEGRYPDVMIACVGGGSNAMGTFYPFRNEKNIAMYGVEAAGHGMNTSEHSASIN
                     GGKAGVLHGNKTYLLQDAHGQVRDAYSIAAGLDYPGVGPEHAYFHATKKVKYVTITDK
                     EAIKAFSFLSLKEGIIPALESAHAIAYAMKLAPKMTKNKIIVICLSGRGDKDAQTIIE
                     KGVMG"
     gene            complement(31839..32501)
                     /locus_tag="PLG98_01495"
     CDS             complement(31839..32501)
                     /locus_tag="PLG98_01495"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOE31954.1"
                     /translation="MTQVKICGIASKGDAFAAVNCGADALGFIFYPPSPRYIKPDDAE
                     KIIRVLPDKVVKVGVFVNEQPKNIKKLVETCGLDMIQLHGDESPDDCRLFPSQVLIKA
                     VELKNEFDLTPACAYPVAAILVDNRHAGRYGGTGKKSNWDLAAGLRGKIPLILSGGLN
                     AENVADALKTVTPDALDINSGVEASPGKKDHQKLARIFDTIRAFDTKHENFQSIFKKR
                     DT"
     gene            complement(32503..33273)
                     /gene="trpC"
                     /locus_tag="PLG98_01500"
     CDS             complement(32503..33273)
                     /gene="trpC"
                     /locus_tag="PLG98_01500"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOE31955.1"
                     /translation="MLDKIIETKKEEVAQLKKETTEAQLKKEIAGFAPCRDFKKALTS
                     SDCNIIAEVKCASPSRGRLVTDFDPLRIARIYEQNGAAAISMLTDEKYFSGHKNYLKQ
                     IRPTVQLPLLRKDFIVDPLQIFETRIIGADAVLLIVRVLGEKLADFISLSGEVGLSQL
                     VEVHSQEELDLALAAGAEIIGINNRDLDTFITDIETSVKLKKRIPENNLVVAESGIRK
                     RKDIELLIKAGIKTFLIGEHLVTAPDIGKKLRELKGES"
     gene            complement(33487..34497)
                     /gene="trpD"
                     /locus_tag="PLG98_01505"
     CDS             complement(33487..34497)
                     /gene="trpD"
                     /locus_tag="PLG98_01505"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOE31956.1"
                     /translation="MIKEAIEKATMKTDLPEAEMMAAMDEIMEGVATPAQIASLITAL
                     RMKGETVEEVSGAARIMRQKATRINACSSTIIDTCGTGGDKLNTFNISTTAAFVAAAA
                     GLTVAKHGNRAVSSGCGSADVLEALGVNISADQEIVEECVQQIGIGFLFAPKLHGAMK
                     YAIGPRREIGIRTIFNMLGPLTNPAGATAQLLGVYDAKLTEMFAHVLKNMGTKKAFVV
                     HGLDGLDEATITGETRVAELRDGIVRTYNINPVDYFGRLDSLESIRGGDPSANAQITR
                     DVLSGKNGPCRHVVLLNAALAIVAGEKAKDIKEGLAIATDCIDGGAAVKKLQGLIEMS
                     NS"
     gene            complement(34513..35082)
                     /locus_tag="PLG98_01510"
     CDS             complement(34513..35082)
                     /locus_tag="PLG98_01510"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOE31957.1"
                     /translation="MILMIDNYDSFTFNIVQYLEQMGEKVNTYRNDKIKTDDIRRLQP
                     RAIFLSPGPCSPAEAGVTVEVIRKFYQDVPIMGICLGHQSIGYAFGATVSRAGRIMHG
                     KISHVKHDGQTIFAGLPNPFPAGRYHSLIVYPETLPDCLEISALTDEGEIMGLRHKKY
                     PVEGIQFHPESVLTPQGKRIIRNFLIYTK"
     gene            complement(35172..36668)
                     /gene="trpE"
                     /locus_tag="PLG98_01515"
     CDS             complement(35172..36668)
                     /gene="trpE"
                     /locus_tag="PLG98_01515"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOE31958.1"
                     /translation="MYQPNFAEFEKLAQKGNLIPVYREILADVETPVSVLMKLRKKDH
                     VFLLESVEGGEKWGCYSFLGTDASVVFRVQGDRVIIDEKGNVTTRHHHGGPLNFLREL
                     LNRYKPVALPGLPRFYGGAVGYLGYEMVRYFEELPPSPPDDLNLDDAVFIISDSLMIF
                     DNVRHTIKVVACTFTEDEESLEAGYAASCRKIEEMIEIISASASHKIAAATEGDVNFQ
                     SNMTCHQYKDAIEKAKEYIAAGDIIQVVLSQRFETEYLNSSIDLYRALRFINPSPYLF
                     FLKMDDLTLIGSSPEVMVRKEDDTMELRPIAGTRPRGRTEQEDRALSDELLSDEKERA
                     EHVMLVDLGRNDLGRVAQTGSVQVNQFMMVEKYSHVMHLVSNVRAQLTKGKDAFDVLS
                     ATFPAGTLTGAPKVRAMQIISELEKVKRGPYGGAVGYFSFSGNMDFCITIRTMILKNG
                     RIYVQAGAGIVYDSIPESEHQESLNKARGMLQAIKLAAGGFEISTNQA"
     gene            complement(37044..37235)
                     /locus_tag="PLG98_01520"
     CDS             complement(37044..37235)
                     /locus_tag="PLG98_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31959.1"
                     /translation="MKKIRRSLKPAGGSTTRVKPQRENVWCERYGYFIDLDACRARSM
                     QNKACRRCFPSLIQVPLPF"
     gene            37364..38824
                     /locus_tag="PLG98_01525"
     CDS             37364..38824
                     /locus_tag="PLG98_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOE31960.1"
                     /translation="MMLLESFLSAVKSIRVQDVLDVMIIAIMIFALLTWFKTRASRFV
                     LIGIILLGGVYIAARFFQLYLTTIVLQSFFAILLFVLVVIFQEDLRRFFERLAMLGSL
                     GKKFPEISGLKSTAEIIAQTAANLAKKKTGALIVLRGNDPLERHINGGTKLDGVVSGA
                     LLESIFDPHSVGHDGAVIIEDNRISQFGCHLPLSANTSKYSNLGLRHTAALGLSERSD
                     AICVVVSEERGTISLAYMENLTVVPSAAVLHETLEQFYIRNTPTKKSHPALNWLKENT
                     LEKVIAIAMACILWMAFGYQRDIVRRDFIVPIEYKNIPRNWQIDEMQVTDARVVLKGP
                     EQAFRLLDERSLKLSLDLSGIADKKREFNLSADMINVPSNLTIADIVPAKIHVTASKR
                     IPWTFPVHVNTQNALAHHLSLQRLSVAPSKVRVLISAQTDPDQIKIETEPVDLKRILS
                     TTTVDTKIVLPIGVALPEGQSPAARVQIKVKQKASS"
     gene            38836..39897
                     /gene="corA"
                     /locus_tag="PLG98_01530"
     CDS             38836..39897
                     /gene="corA"
                     /locus_tag="PLG98_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOE31961.1"
                     /translation="MTSKYHKRSRKTGLAPGSLVHIGSPYAEKSKITLMQYNEGFFEA
                     KDLESLKDFQADKDKEIITWLNVDGLTDIQLFEEIGRIFGLHPLVLEDILNTAQRPKM
                     EDYGGYIYLVARNFHDQENGSLLFEQISIILGKNFILSFREKESNLFKSIREKISQNK
                     GRIRKENTDYLAHALLDNIVDNYFVILENLEEKIEALEDDLIRQPDSSSLQAIHMLKR
                     KLILLGKSLWPLREAISALERSDSALINKTTVVYFKDIYDHTIAIIDTVETFKDMLSG
                     ILDVYLDTVSNRLNEVMKILTVIATIFMPLTFIAGIYGMNFKYMPELDMRWGYFGILG
                     VMLLVALFMVRYFRKKNWF"
     gene            39969..40448
                     /locus_tag="PLG98_01535"
     CDS             39969..40448
                     /locus_tag="PLG98_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOE31962.1"
                     /translation="MSVFAASDIVEVAIRIEENGVNFYKFAEQIAKKEDEKKLFAGLA
                     QAEVAHKKTFEKLFAGMEKSNLQESYEGEFGEYLRAYVDNNLIFTKEIMDKQLAKVTD
                     TTSALEFAIQRELDSILYYHEIKKMVPAAQHDALEKIIEEERKHFQALTAMKKRLAC"
     gene            complement(40724..41494)
                     /locus_tag="PLG98_01540"
     CDS             complement(40724..41494)
                     /locus_tag="PLG98_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31963.1"
                     /translation="MSNTNDQNLSQIIIKLKPKLIFLYIFVPIFFLVMAHILGQLAIF
                     NIIPCFRGYDLFSVFFNLDGEANIPTLYTILEWFLCVIFLVLIAFFERNTQSSRYRYW
                     LGLAIIFSFLLIDEFISFHEISDHILALFNIKIVLFPTNAWVLIYLVVLAPISLVYLR
                     FMIHLPKKTKYLFVFSFIIFMLGAAGIEALELRHVEIYGQDKYYYLYFVIVEETLEMM
                     GLLIFIYALASYLANELGISQIKILPEKISHESTDKSA"
     gene            complement(41503..42867)
                     /locus_tag="PLG98_01545"
     CDS             complement(41503..42867)
                     /locus_tag="PLG98_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE31964.1"
                     /translation="MQKLSILIVDDEKSQRELLRDFLRSEGHTVTEADNGEKAIKMVL
                     GGHFDLILLDYKMPGMNGLEVLKEVKRINPEIDVVIITAYGTVETAVDALKAGAIDYI
                     TKPIVELDELIILVNRIAERRQLIQENRLLKDDLSKHGITADKIIYQSRQMAELINLA
                     GRVAPSKASVLIQGESGTGKELLARLIHQLSPRADKPIIIVNCVALHENLLESELFGH
                     EKGAFTGASSRRIGRFEEADGGTIFLDEIGELSPTIQVKLLRFLQEREFQRLGSNVNL
                     QVDVRFISATNRDLEIQVAEGTFRDDLFYRLKVVTMSLPPLRERKEDLPVLIDYFIDK
                     FAKENGKNIKGFTAEARDLLLKYDYPGNVRELENILERAVVIAREQYITTNDLPFKSD
                     FLLEDSEKKPFGALKDSLDELEKKLIIEAMEKTQNNQTRAADLLGISERMLRYKLKKY
                     GLKG"
     gene            complement(42889..44676)
                     /locus_tag="PLG98_01550"
     CDS             complement(42889..44676)
                     /locus_tag="PLG98_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE31965.1"
                     /translation="MRLFRKKDIVVHRKAHNYNDPFLQPLAVAIVSAIFVALILITGF
                     LELRRSESNLIAFMENQALSTIGVLQRLTEENLKSIIGVPDKNAPHEKTSGRDEASYS
                     KQWVIQAITELAKDLDEQWRKGDMTDESLRQFASDNNFSYVGMLNAQGNAVFQSGPLQ
                     RDLLEENELTHKGRKLTTLELISKIGEKQGVGFIALSRKDNTGAVIISLDKSGSVYWI
                     LKIAGERGMDKIGEGHGIVYMQIFNDRDKLLSSVGQPAPALNHRIFNLKEILSGKIKI
                     VSRKVDYADNKILDLAAPLYLDKKIVGIVRIGLDRNSMDKSLVENRQHIFVFSIFIVL
                     IALLSMWLLYHDQNRHLAGIVEMERQLEKAERLSSLGQLAAGVAHEIRNPLNAISMAT
                     QRLKRDFVPSDPQKVDDFQNLSGIIRDEIKRLNGIIEEFLNFSKSRRLDFCKFSITEL
                     LQKIVSLMKEEALLRGITLETTWKQESVPISMDVNKLQQAFINLIKNAMESITAEKGR
                     IVISVDKEGKNYIVVKISDSGCGMTEEELEKIFNPEYTTKEKGVGLGIPLAFEIIRGH
                     GGDIRVTSRKNKGTTFEVILPRERLNEVS"
     gene            complement(45419..46768)
                     /locus_tag="PLG98_01555"
     CDS             complement(45419..46768)
                     /locus_tag="PLG98_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOE31966.1"
                     /translation="MKTLDLFDQGNSGNVKDAAPLAERMRPQNIYEYIGQKHLLGEGS
                     LLKRAIESDKLFSMIFWGPPGSGKTTLARILANETKSKFVSFSAVLSGVKEIRAVIDE
                     ARELLERKSIKTILFVDEIHRFNKAQQDAFLPHVESGLITLIGATTENPSFEVIAPLL
                     SRTRVLVLKPFSEENLLAILEAALKNKQKGLGNIDVKIDANVLRYLVSLADGDARSAL
                     NNLEVLVSMVQNLPDDQRAISLKFAQDALLKKSLLYDKDGEEHYNLISALHKSLRGSD
                     PDAALYWLGRMLAAGEDPLYIARRMIRFASEDIGNADPQALVVAVAAQQAFHFIGLPE
                     GELALAQAAVYLATAPKSNALYVGYGKVKNTINTKGYLPVPLHIRNAPTKLMKDLNYG
                     KDYKYAHDYEESYVPQDYLPEEIKGELFYQPKGVGFEKTIKERLNYWRNKKKEGEKE"
     gene            complement(46765..46968)
                     /locus_tag="PLG98_01560"
     CDS             complement(46765..46968)
                     /locus_tag="PLG98_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31967.1"
                     /translation="MTFAYCLREGGNLPCVRIIRCWSPVFDIESFLKGHLSEKRWLKF
                     INTKAPDKITSLIELIEAAKAKK"
     gene            complement(47104..48468)
                     /locus_tag="PLG98_01565"
     CDS             complement(47104..48468)
                     /locus_tag="PLG98_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOE31968.1"
                     /translation="MEQVKVVLQDREIPKKWYNIMADLPTPMKPPLHPGTGQPVTPQD
                     LAAIFPMNIIEHEASNKRWIKIPEEVLQKYLLWRPSPLYRAYNFEKLLNCPVKIYYKN
                     EGVSPAGSHKPNAAVPMAYYNKVAGTKRITTETGAGQWGSALAMATQMFGLECRVYMV
                     KISYEQKPYRRLMMNTWGAECIPSPSTLTQSGRDFLAKYPDSPGSLGIAISEAIEDAV
                     TSKIKTHYALGSVLNHVLIYQSIIGLEAEKQMQKLGIYPDVVMGCCGGGSNFAGIAAP
                     FVRDKIHGKDVKIVGAEPSSCPTMTKGPFAYDFGDLAQKTPLLPMYTLGHDYVPAPIH
                     AGGLRYHGMAPIISHLVKEKLIEPRAYDQLKTFDAGVKWARSEGFIPAPETNHVLAAV
                     VDEAMRAKKEGKEKTILFNWSGHGIIDLAAYDAYFAGKLKAYELPGHEIERALKAIKN
                     FPKP"
     gene            complement(48741..49688)
                     /locus_tag="PLG98_01570"
     CDS             complement(48741..49688)
                     /locus_tag="PLG98_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOE31969.1"
                     /translation="MEKIRLGISSCLLGNNVRYDGGHKLDRFLTDTLGKYVDYVPVCP
                     EVECGLSIPRPSMRLEGHPDAPRLMVTSTRQDLTERMETWAKKRVTELEKEDLCGFIF
                     KSDSPSSGMERVKIYNEKTMPVKNGVGLFAAMFMKHFPLLPVEDEGRLHDPGLRENFI
                     ERIFALKRWRETLSAKNFKKSLIDFHTKHKLLILAHSTKHYQMMGKLVAAQKNFAGPD
                     IQKEYQALLLESLKVKATPAKHVNVLQHMMGYFKDQLSGDEKKELLELIHAYRDGYIP
                     LIVPVTLISHYVRKYDEPYLKDQVYLNPHPAELQLRNHV"
     gene            complement(49882..51297)
                     /locus_tag="PLG98_01575"
     CDS             complement(49882..51297)
                     /locus_tag="PLG98_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE31970.1"
                     /translation="MDLIQKYLMKDIDVLLNCIDEAILIVDRDANILKYNETFSRLTD
                     LMGRSLVGMNLKDIVASGILRESAALKSLEVKKKIDMNLTYETGQTATWTYIPVFDER
                     GELVMTVGTGRDMTKLVTLEKKLKISETIISQYEGRNLKMAGDAGQVEIIYSSSVMQQ
                     VIRLGWKAAATSSPVLIWGETGVGKEIMAALIHQLSARSAKPFVAINCASIPDELLEA
                     ELFGYEEGSFTGAKRSGKKGLLEEAHEGTLLLDEIGELPLKMQSKLLRFLQEGNFQKI
                     GSNKTQAVDVRILCATNLTMDQLMNSKQFRQDLFYRIAVIPVFIPPLRDRQEDIPPLI
                     LHFIQIFNTKYKTDIKLPAKLMNRLYTYEWPGNVRELKNVIERMAIMHASQELTEEDL
                     ELILNINPRQASSDRSLPAPEPAMPVLPPSLPKAMDQFEEKWIRQAYQMTGSIVGAAK
                     LLGINPSTIHRKLKKGILRLD"
     gene            51476..53185
                     /locus_tag="PLG98_01580"
     CDS             51476..53185
                     /locus_tag="PLG98_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE31971.1"
                     /translation="MSNQKPWLKFYKNVPETIDYPRVTMYEALMQTVERYPDRIAYDF
                     FDYTSTYRQFAQDIDICANALASLGLKKGDRMTISMPTSPQGIICFYAANKLGVVASM
                     IHPLSTPAEIAYYLNVSKSRVALTLDLFHNSFKNLPEKTPLELLILTEIFDQLTQAQP
                     MAEKPLPEEGPAVRWWKELMTAAHPQAPKADMHTDEMAVILYSGGTTGFPKGIMLSNF
                     NFISEGLMVANWGGGELMAEDSSVLAILPIFHGFGLGVCCNAAFMGGSKSILVPQFTP
                     ENVADLIRKKRPSIIVGVPTLYDALSKNEVFQTTDLSCLKNTSSGADSLPRTVKDRFE
                     EVVKRQNGNVQLVEGYGLTEAVTAIMSTPIDGYREGSVGIPFPDMLAKIVGIGTIDEV
                     PVGQEGELCLHGPAVMLGYLEQPEETASVLKTHADGKVWLHTGDIFTMDADGYFYYKL
                     RLKRMIKSSGMNVYPVQVEACLDQHPDVAAVCVIGVPDEAQVEKVKAFVVLKDATKAS
                     AEMEKELINFSRKHLIKWSCPREVEFTDKLPLTKVGKIAYTELAKQEIEKLRKAGKYT
                     GGK"
     gene            53185..54306
                     /locus_tag="PLG98_01585"
     CDS             53185..54306
                     /locus_tag="PLG98_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOE31972.1"
                     /translation="MNHLEEYRRAVSDPSDYVQQLKKASGKKIIGYTCSYTPEEVIMA
                     AGAHPLRLFGTKQHISLADSHLQSYCCSVVRGILEEGLSRRVDYLDGMVFPHTCDSMQ
                     RLSDIWRMNIPFGFHLDITLPVKLDTASAKDYMIDVLKKFRDDLGGKLNVEISDDAVQ
                     KAIATTNAIRQSVRAIYDIRNLHPRLMPGEDLYNIVRASMIMDRDRLAVMLAETAAEL
                     KERATSAKPASVKRILMAGGMCNQPDVYAMIEESGGEIVWDDFCTGARYFTGLTDAGS
                     DPLSSIGERLLNRVVCPAKHADLDGRARHIIQLAQEKNAQGVIFLLPKFCDPHAFDYP
                     YLKERLEKAGIPLMNVEVDDSLPAEGQLKTRFESFLEML"
     gene            54318..55556
                     /locus_tag="PLG98_01590"
     CDS             54318..55556
                     /locus_tag="PLG98_01590"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOE31973.1"
                     /translation="MSDTQDTEKKTVSYTKQMRDIMTAYYIDAKTARENNKKVAWITS
                     GGPVEPLIAMDIIPVYPENYGAMIGASKMGVDLCKKAEDMGYSSDLCSYARGDISCAT
                     VKGGPIGGLPEPDMLVCCNNICGTVLKWYEVQARYFKVPLFIFDTPIVHTEFTREMKT
                     YVQRQMEEYIVFLENVSGRKMDRDRMVEVGKLSVEGQQLWQEVLDTTMNRPSPMTAFD
                     AFFYLALIVTLRGTKQAVDFYRGLRDEMRIRASEGKGAIPRERYRLLWDNLPIWYRLK
                     WLSQEFAKHDACLVADTYTSAWCGSMKYIDRNDFIGSAAETYTRIYLNIGVDKMAEIV
                     LSMIDKYQADGIVMHSNRSCKPYSFGQYDIQKIVERERGIPSLIIEADMCDERSFSEA
                     QIQTRIDAFMEMIRAKKGAS"
     gene            55675..57018
                     /locus_tag="PLG98_01595"
     CDS             55675..57018
                     /locus_tag="PLG98_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOE31974.1"
                     /translation="MKVRKIILAVICTLMLAATAVMASGPSLQGEELYKYLSKKTISE
                     GDAAFVEGLNTSSLKLDWSAAQKKDLTGNLRILKVKNAKVGTEALYFIRGSEGDMYVV
                     SLPAEGGRPAAEYEKISENRMMFALETVTGEHEGTTYAFARISGEPKAILFDRIFKIA
                     IVLMLFFVMVGMGLTLKLSDFALVFRKPLGIVIGIVLQYGLMPLVAFGLSYMFGFYQA
                     YPFIFMGLILATASPAGVTSNLLTQFAKGDLALSISLTSISTILAMFCTPLLLSLYGN
                     TSSGASVPALLIAQTIIVLVIVPLAVGMTVRAKFEKAALKAAPIFSILGIITVLFIMV
                     TGVVGNLNALKDTARYSLSFYLAPLILALTGMVIAIVVAKIFRVSPKQVRSLAFETGV
                     RNSALSMTLAILIQDQIGDFYSSLFIVNGVYGLEMYIAGILFVMFFRKFSKETEG"
     gene            57126..57212
                     /locus_tag="PLG98_01600"
                     /pseudo
     CDS             57126..57212
                     /locus_tag="PLG98_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611470.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            57372..57737
                     /locus_tag="PLG98_01605"
     CDS             57372..57737
                     /locus_tag="PLG98_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31975.1"
                     /translation="MKDVTFLTHKGVRILYEDLENAKQEDVLPWIERAKGVIRSQPEK
                     SVLALLNVKNAKFDTEVTAALKEFAKGNEPYVKFAAVYGIEGLKEIIFKSVLTFTGRK
                     NLVLCKTLEEAKDILASKV"
     gene            57986..59050
                     /locus_tag="PLG98_01610"
     CDS             57986..59050
                     /locus_tag="PLG98_01610"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOE31976.1"
                     /translation="MKIIKAQKLAYFDTSHEMPLEIIMAAGFTPYKILGDVHQSNDPA
                     DKHLSNFFCGAAKSWLTEALARSKEWAGIIFAQGCNNTNRHYDIWKVHVQTPFLHWFG
                     APLLDSDAAKKYYRVELQRLISHLENQFNIKITPEKIREAIIKSNEIKKKLQRLSALR
                     AQKDIPNRDYLAAMIKCLTNPVDEVSAWLDSEIAAWNSKPSFPSGKKQFLLTGSDITY
                     VEWMDTLEDCGIRIVRDDLSVGERFFATLIPDKSDPLDALIEYYMNIPKPATRPTIQK
                     RLDYLYKALGETKVDGVLSQNVKFCEPFAYDSVPVNNALKDKGYKVIHLERDFSPAAD
                     QQLANRLMAFVEMMEVGNNG"
     gene            59043..60323
                     /locus_tag="PLG98_01615"
     CDS             59043..60323
                     /locus_tag="PLG98_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOE31977.1"
                     /translation="MAKTNNSAENDRKIKLKTLPHAKDLKWPMLWWFTMGSIYHALPW
                     KKTAWTCAAFPIELMWIYDIFPDHPENMATVAAARKQSQRLIEHAEGMGYSRDLCSYC
                     KTSIAAYDTKIKKTLGGISKPDIVVCTNAICDTHWKWFQIMADKMNVPFFMFDIPKWV
                     SGTDEKTLQGNIDYVVEQFYDLIVFIEKHTGKKMDEKRLYKVLDKSTELSDLWREIFE
                     HRKAVPSPYSGAETSASFFPLVVLPGVQVGINFFKKILADIKNLTAKGEGTMPKDKEK
                     YRVMWEGIPFWYRMRFMYDLAQYGAVITFEPYTYSFAPEKKKCNTMEETLRDVAKLIM
                     DTPYAYNLERRIEHFEKWIDEYKIDGVILHENMSCRPSSAGMVDLAEAIMRDKGIPVL
                     ILQCDMNDPRAYSEGQIKTRVEGFIELMEANKGK"
     gene            60510..61277
                     /locus_tag="PLG98_01620"
     CDS             60510..61277
                     /locus_tag="PLG98_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOE31978.1"
                     /translation="MITAGIDIGSITTKAAIFADGKITATKVIFTGYNAEAAGLKVYE
                     EALKEAGIDKNAVAKIVSTGYGRNSVKFADKSYTEIMCHATGAHFLNPHIRSIIDIGG
                     QDSKAILLDDKGKVKNFVMNDKCAAGTGRFLEVMARAMEVNLDEFGAMSINSNQPAKI
                     SSLCTVFAESEVISLIAKGEQRKDIIAGIHESIASRISSMIGRLGIKEPVMITGGVAK
                     NVGVVHALEKKLGLKIEVSPYAQVNGAIGAAVLAASL"
     gene            61311..61787
                     /gene="tadA"
                     /locus_tag="PLG98_01625"
     CDS             61311..61787
                     /gene="tadA"
                     /locus_tag="PLG98_01625"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOE31979.1"
                     /translation="MSTDYDFMKIALEEAQSAYRRGEVPVGAVLVREGNVLARAHNSP
                     IALNDPSAHAEMQVLRQAAEKIGNYRLAGTELYVTIEPCIMCAGAIIHARVERVIFGA
                     RDPKFGAVVSLYNILDDKRLNHQVKVTEGVLKEECGEIISRFFREKRVTSAPASAK"
     gene            61796..61889
                     /locus_tag="PLG98_01630"
     tRNA            61796..61889
                     /locus_tag="PLG98_01630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61831..61833,aa:Ser,seq:cga)
     gene            62018..62111
                     /locus_tag="PLG98_01635"
     tRNA            62018..62111
                     /locus_tag="PLG98_01635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62052..62054,aa:Ser,seq:gga)
BASE COUNT        15991 a        15003 c        15652 g        15977 t
ORIGIN      
        1 ccagtttggg aaccggcata tgcaaatctt cctgaagggg atcttccgcc gcccggccgg
       61 cagtagatat ttcccgcgga tccggcatgc attgggcgta aatgggatcg tgaaaatcct
      121 ggtgttggat aagcgataaa tagtaaggcg ttacggccat cggataaaca gccgttaccg
      181 actggagggt ttcccggtgg ctcgcggcgg tttgcggctg cgcgcacggg aggagcggat
      241 gggtgaggcg gtttttgatc tgccagcgcc agtcggccca gtcttttacc gaggtttttt
      301 gaatgaaagg tgaaacagtt tttgtcttca taaggatctt tttttaccaa acaatttgag
      361 gctggctttg tatcacaaaa ttaggtgctt gcaaagccgg cgaattgcga ttagaagaaa
      421 aacacagcgg gcctgcaata cagatggcca attttaatta cgaggcaata ccgtgaaaga
      481 tatgcagcag cgcaaagaaa acgtggcaat gttgtcggtt gcttccaatg cgtttctcgt
      541 tttattcaaa gtccttgtcg gctttgccat cggttccgtt tcgatcattt cggaagcgat
      601 tcactccggt gtggatctgc tggctgcagt gatcgccatg ttttccgtca aaacttccag
      661 cgttccggcc gacgaacgtc atccgttcgg ccacggcaaa attgaaaacc tttccggctt
      721 tgtggaagcc ctgctgattt ttggcgcggc gttttggatt atctttgaag cgctgaaaaa
      781 actaaatgcc ccccaggcca tggaaaatgt cggctggggg gtcggcgtca tgttgttttc
      841 tgccgtcatg aattttattg tttcgcaaaa actttttaaa gtgggcaagg aagccgattc
      901 cattgctctc caggctgatg cctggcattt gcgcacggat gtgtatacgt ctgccggtgt
      961 catgtttggc ctgacgatca tctggctggg tcataaattc ttcgccgacc ccaatattta
     1021 ctgggttgat ccggttgccg cgatcttggt cgccgcgctg attatgcgcg tcgcatatcg
     1081 tttgaccgtt gacgccctca gtggcctggt tgatgtccag ttaccgaggg aagaagagaa
     1141 ttttatccgc agcatgatca gcgtggaaaa ggccgtctac ggttttcatc aattgcgcac
     1201 gcgcaaagcg gggcatgttc gctttgtgga atttcacatc aaagtcgatc cgcagatgtc
     1261 cgtccacgat tcgcacggca ttaccagaat tttgaagaaa aacatcaggg ctcggtttcc
     1321 ggaatccacg atcaccattc atatcgaacc ctgtgatggt gaatgttccg atatgtgtca
     1381 ggccggttgt ttattgcccc gggagaaaag accggataaa ggcatgatcc gaccgcaacc
     1441 gatcggacat gatcaatcat cgtgaagagc tgctgtttat aatagacaaa tcggcggggt
     1501 gacgggcggc agtttatcgc cttcgtacag gaccttttcc agaaaaagac cggaaggcgg
     1561 ggcggtgaag tgcgcgggca gctctgaaaa ggatgtcatc attttttcga catcgggcct
     1621 ggtgaggttg ccgcggccga gctcaacggt cacgccgaca atgcgccgga ccattttcca
     1681 gagaaaatgc gaccctaaaa tgcgcaccgc aatgatattg tcgaattctt tcagccatag
     1741 gccttcaatg ttgacttttg tggaagcttc cttgtccatt cttttgtcgg cgaaggaggc
     1801 aaaatcatga aagccctgaa aaacatccag cgtctgtttc attttatcgc aatcgagtct
     1861 gtctttgacc caccagacat atcgcttgcc gaaagccgtg cgtcttcggg aaatcaaata
     1921 gagatagctt cggctgagtg cgtgatggcg ggcatgaaaa cgcgcgggcg cattttccac
     1981 ccttaagatg ttgacgcttg acggcaggtg atcgttcata cccgtgcgta aaatgtccgg
     2041 atggactttt ttgggagatt cgagatgggc cgcttgctcc agggcatgta ccccggcgtc
     2101 ggttctgccg gccccctgta ttcttaccgg cacgtccaga aaactttgcg ccgccttgat
     2161 taaagtttcc tgaacgctgc gggcgttttt ctgcgtttgc cagccgcggt aaccggttcc
     2221 gtcatattca agcagaattt taaatttgta cgatgccatt ttcagtgaag gtgttgttta
     2281 atacgtcgtc ccttatgacc tattttctta aacagcggcg tcaacgggaa gacatgctat
     2341 ttactttgcc ggcgccagta tcgtcatggc ggaaagattt ttgatcagca aggcatcggg
     2401 atcgatgttt gttaattctt tgccgggatg caaggtcgtg atgacggcct tgccgatgat
     2461 cttgcgtcct tcctcgccgc gggcgtctgt gattctcagt ccccagacat tgtgaaaaat
     2521 cagtgcccgc ccgtttcgtt cgccgatgta aagcatgata tgtcccctgc gccacagcag
     2581 gctcaaatag ggaatgccct gttccagaat cactttttct ttttcttgcg gttccatagc
     2641 ctccaggtca atataaatgc cgattttttt tgcctgatcc gccgaatgtc tgggcagcca
     2701 gatgccgaaa ggagcaaaaa aatcccgcgt cattgcggaa caatcgcggt ttccgtaaag
     2761 accgccccag ccgtagggtt cgtcaatcag ttcattggca attctggttg cgtttgcata
     2821 ggtaaacggc aacggtttga tgttcatgat ctcccgcgaa atgaaaacgc gttggataat
     2881 ggcctgatgg tttgcgtccg ccgaggcgat gagaatttcg agtttatccg ccatctcggt
     2941 gatcagagga aacatttgtc cgatagaggt ccggagactg aaattgccgt ttgcgtcgac
     3001 gatcgggatt ttatcccggg tgacggtggc atagcgggag gtttgccatg tctgcataaa
     3061 gggatcgtca acgcgtgcaa aatcctcagc cggaatccat ccgaaggcaa agctggtttc
     3121 caccagggcc catgaccggt ccctgctgat gtggcatatc agaatcggtg tgttggccgc
     3181 cactgaagat atctgaagat tgtcaaacgg ccagccgttg atgtcgccgc ccggtttata
     3241 aaaatgtgga ccgttcgtgg gcagcatgcg caggttcgtc gatcggacgg tgatcgccgg
     3301 ctgcagcgta ttgggataat tttccagcca ggcgttctgc tgaagttttt tcaaccatgc
     3361 ggtggaatgc tttctcttgt tttcgccata gcccggatgg gcggcaaatt gtgtgaaaat
     3421 ggcagacaat ctttccggca gtgcgtgcac cggttcagga tggtgccaca cggaaaaaaa
     3481 cgttttgtcg taatgttcat tcatcgcagt ttgactatca cgggtcagaa ggactttgtc
     3541 tgccggcacg tccgccagac gggcggtgtg atcctggggc aggtcggtga catcttttac
     3601 gtcctggagg gtatcgggag gttgcaaata acaggacact aaaaacagcg ctaaaacaac
     3661 gcagacgtat tttactttca tgcccgatcc cttcaattta ttctggcgta ttgtatagtc
     3721 ttttttcatg ttttgggcaa gatgaaacgg cgaaaaattt tctgtatttt ataaataatt
     3781 tatggaataa gggatcgaac cctgctatac aaacttttgg attttcgacg ggcagggcga
     3841 aaagttttaa aaatacggcg ccgtttacgg gtagaagaag gagatgaacc atggatagtt
     3901 ttgtgcccaa aaagattttt tttacaaaag gagtgggcac gcacaaagac gaattgcatt
     3961 cctttgagcg tgccttgcgt gatgccggaa tagagaaatg caacctggtg caggtgtcca
     4021 gcatctttcc gccacgctgt aaaatgatct ccaaaactca ggggctgaaa caattgacgc
     4081 cgggcgccat cacgtactgc gttatgagcc gttgctgtac gaacgaaccc aaacgactca
     4141 tggccgcttc ggtcggttgc gccattcccg ccgataaatc atcctacggt tacatcagcg
     4201 aacaccatgc tttcggtcag aatgaaaaac aggccggcga ttacgcggaa gatcttgcag
     4261 cggccatgct ggcctccaca ttgggcatcg attttaatgt tgatgagagc tgggacgaaa
     4321 agaaggaaat ttttaaaatc agcggcaaga tcgttcgaac gctaaatgtg acccagtcga
     4381 caatttgcaa agacaacaaa tacaccaccg tgattgcagc ggcggtattt gtgttttaaa
     4441 tatttttaaa agcgtataaa cggcgtatga atccccgaag ggagacatac accggtgaca
     4501 tgcgaccttt tccccggtgc ggactatgac ccaaacggaa agaattctgg atattccgtc
     4561 tctgaaccac cacgatatcg gcggagcgca tttaccgtat ctttattacg agggagagac
     4621 tccgcaaatt ctttttgccc acgccaccgg ttttctaccg tggctttggc atcccatcct
     4681 ggaggagctg acgccgccca ggaggcaggc gtgggcgcca tatatatgta attaccgcga
     4741 ttgcgatccc gaaagcggcg ggctttcctg ggatgtgatt gccagagacc tgatcagctt
     4801 ttgcagcgtt cagaatatca gcgataatct ggctgtcggg cattcgatgg gcgccacggt
     4861 ggtgacgatt gcctccgcgc tttttggtct gcagccccgc gggatgattc tgattgaacc
     4921 cattttcctt ccggaagatt tttataccat cgatatccag ctcaaagatc atcccctggc
     4981 ctctttgtcc atccgaagaa aaaacggctg gaaggatgaa aatgccgcgc tggagtatct
     5041 gaaatcaaaa tcgctttttg cgggctggga cgaacagatg ttgcaatttt atctgcaata
     5101 cggcatggaa aaacaggccg gcggcgattt gaagcttacc tgtgcgcccc gcaacgaagc
     5161 ggctcttttt atgggcggta aaagcaccaa tccctggccg ttgctggaaa agttgacctg
     5221 tcccgtcctg attctggaag gggaaaaaag ctccaacaga gagtttatcg atctgcccaa
     5281 ggcagcatcg cttctccggg acggccggta ccagttgatt cccgatgccg gtcatctgat
     5341 tcccatggaa aaacctaaag aaatcgccaa aattataaaa gaatttctgg cggacgtctt
     5401 ttaggaaatc attgacgaaa tgctttaaat atgtaaaatt tttacgctag cattccggga
     5461 acaatgaaag ggaagaaatc tgttgatagc tttggatgat tacaagcaga ataggactgg
     5521 agacacacag aggttcaggg gaacggtgag caaaccattt gccttgatcg ggcggaatgt
     5581 catccggtgg attaacagca tcggagcggc cactattttt ctggtgctgg ctttactcaa
     5641 aacctttcat cccaagcagt ttcaaaaggt cattcagcaa atttattata tcggggcgcg
     5701 atcgacgacg attatcatgc tggtcagtct gtttaccggc atggtgctcg gactacaatc
     5761 ctatcatgcg ctggtgcagt tcggcgcgga aggatcgctg ggaacgctgg tctcgctgtc
     5821 gctgattcgg gaactggggc ctgtgctgac cgcgattatg attacggcca gggccggatc
     5881 ggcaataaca gccgaaattg gcgtgcagcg gatttccgag cagattgacg ctttggaaac
     5941 catgcgcatc gatccttttc gctatctggt gactccccgg atcaccgcgt cgatcgtcag
     6001 ttttcccctg ctaaccactg tattcgatct gatcggcatt atcggcggtt atgtcagcgc
     6061 ggtggctctg atgggattga atgccggcgt ttattcacac agcattcggt cctacgtgga
     6121 tatggaagac ctgaccaacg ggtttgtcaa atcgcttgtg ttcgcggtga ttgtcgcgac
     6181 cgtgtgctgt tatcagggat attttgttca tatgcgcgag gacagcaaag gcgccaaggc
     6241 ggtcggtctg gccaccacct cagcggtggt tatttcctgc gtattgattc tggttgcgga
     6301 ttacgtcgta acgtcactgc tgatggtata gaaaaaatat gaacacaccg ttgattgaat
     6361 tcaaaaacat caccaaacga ttcggagaac agaccgttct cgatcaggtc agtttgcaga
     6421 tttacgaagg ggaagtgacc accattatcg gcctgagcgg ttcaggcaag agcgtgctgc
     6481 tcaagcacat cattggcttg ctcaaacccg atgaaggaac cattcttttc cggggagaac
     6541 cgatcaacga catgaagaaa acagaactgg ccgaagccat gtccaaaatc agctacatgt
     6601 ttcaaagcaa tgccttgttc ggttccatga cggtttatga aaacattgcg ctgccgcttc
     6661 tggaaaatac ggccatgaaa aaaaacgaaa ttcatgaccg ggtcatggcc agaatcgaac
     6721 agacggaatt gacagaagcc gcacagaaat atccttcgga gctttccgga ggcatgcaaa
     6781 aaagagcggc gctggcacgg gcgttgatta ccgatcccca ggtggttttg ttcgatgaac
     6841 cgacaaccgg tcaggaccct gtccggaaaa acgcgatttt gagcatgatc gcccagtatc
     6901 agaaaaaatt taattttacg gcggtcatgg tcagtcatga aattcccgat gtgtacttta
     6961 tttccaaccg gattctcgcc ctctatgaca aaagcatcgt ctttcaggga acaccggagg
     7021 aattgagtaa ttttgatcat cctttcaacg atgaagtgat ccgcagtctg gaaggtttgc
     7081 aaaaggaatt aaccggtctt tattccagaa gacaatttaa ggttttgcac cgttcccagt
     7141 tacaaagccg caagtccgac gaaggctatt gtgtggttgt gtttgattta tcggatatga
     7201 attcgattgt ttccgccatc ggttacgaca gggcgcagga aaccatccat tcgatgggaa
     7261 agtacatcga taaacatttt gatgccatcg gcggtttctc cacccgtcgt aaaatcaatg
     7321 aattcgtgac agtgcttcct ttttccgata ttgccgagac ggaaagcatt gtcaaagact
     7381 ttatggagga tttccaaaaa agtggcatga gtgagatatg gagcgaagct caaaaaaacg
     7441 atcctcagac gaggtgcgtt gatttttcag tcaaagccgg tatggcggaa gggacaccgg
     7501 tcgctgaaat tgattcaatt gtaaaactgg ccaaagctca tcgaaaagaa atcggacggc
     7561 tgcaatgtgc cgtcaaggag taagcacatg aaaaaatacg agattgaaac tgtcgtggga
     7621 atttttgtgt ttctcggtct tctctgtatc ggatatatga ccgtgaaact cggaaatgtg
     7681 tcttttttgg aagccgcgtc ctatcccctt gaagctaaat ttacgtccgt caacggttta
     7741 cggatcgaca gttccgtaaa ggtcatgggt ctgactgtgg gcaaagtgaa acaaattgcc
     7801 atggatcagg acaatcaacg ggctgtggtg aaattaaata ttcaaaaagg catcaagatt
     7861 tatgacgatg ccatcgcctc gatcaaaacg gaaggattga tgggagacaa atatgtcagc
     7921 atcgatccgg gcggcggagg ctctctgctg gagcccggtg gaattattct ggaaacgcag
     7981 tcaccgttgg acatagagac gctgatcagc aaatacgctt ttggtgatgt taaaaaacaa
     8041 tagatcggtt attacgacgt tgagggagga aaaattcatg aaaaaacttt ttacccaaac
     8101 agccatcatc ctggtgattg taatgttttc tctgcctgtt tatgcgggcg cggcgctgga
     8161 ttccgttcag aaatgcgtca acgaggtcat cggtatcgtg ggcgatccca agctcaagag
     8221 cgagtcggcc aaggaactga aaaaagaaaa actccgggtt ctatacaaac agatgttcga
     8281 tgaaattgaa ttttccaaac gcaccctgac gcgcaactgg aataatttta cgcctgccca
     8341 gcggacggaa tttgtcaatc tttttgagca ggtgctggag acagcgtacg gcgataaagt
     8401 gctcagctat accaatgaaa aggtcgtctt ttataaagaa accacgcttt cggcaaccca
     8461 ggcggaagtg caaagcaaga tcgtcacctc ttccaaggaa attcctgttt tttaccgcgt
     8521 tattttaaaa gacggaaaat ggaaagtata tgatgtcgtg gtggaaaacg tcagcctggt
     8581 tcagaattat cggacgcagt tcaacgatat tttgtccaag gacaaaccgg aaaaattgct
     8641 ggaagttctc agaaacaagg ttaagcagca ttaaaaaacc tgctgaccct gcggctgtgt
     8701 tgcagcacgc cggctgataa cctttcatac ggtcggatga catgaaatct cgtttggtat
     8761 ttctcgtatc gtttatatgg atgctgtcgg ggggcgcacc cggcagcgct catgcatttg
     8821 ttgatctgcc gccggagcag acatctccgc cgcctgtggt cctgacagtc tccccggacc
     8881 ggcccgcaaa tcaattcgcc gcggccgctg tggttgatcc catggaagag gaatacacgg
     8941 gggacgaatt cgatgactat gcgggggaca atgctaaaga agaaaaagtg tccatcgccg
     9001 atcctctgga acctttcaac agggcaatgc atgagttcaa cgataaaatg tatttctggg
     9061 cggtcaagcc cgtagctcag ggctataaag ccgttgtccc cgaacccgcg cgcatcagtg
     9121 tggataattt cttttccaat ctcggatttc ccgcacgatt tttaagctgt ctgctgcagg
     9181 ctgatttcaa aggtgcagcg acggaagcgg gacgttttac cattaatacc gtctggggca
     9241 tcggcggtct gctggatccg tcttccggca aggagttgaa tttacaggag cagaacacgg
     9301 atctgggaca aactctcggc gtctggggcg tgggacaggg tttttatatt gtctggccgg
     9361 tcttcggtcc ctccaccccc cgggataccg tcagcatagc cggcaattat tttctggatc
     9421 ctgttgccta cctgaatccc tggtcggccc gcctgggtgt gaatgtcctg gataaggtca
     9481 acgccgcatc cctgagaatc ggcgattatg aagcgctgaa aaatgcggcc atcgatccgt
     9541 atgtggcttt tcgcgacgcc tacattcagt accgttacaa gcaaattaaa gccagaaagg
     9601 caaaaatttt ctccggatca tcggcagcag aaccgtttcc ggagacggtc cggccggaca
     9661 aggtggatta aatgaaaaaa atacgttgcg caccatattg gatggttttt gttctgacgg
     9721 cgttagtttt ttttacaacc ggtccggcgg caagcgccgc aggattgttc agcgcctttg
     9781 agaccgtttc ccgtcaggac ggcggattga ataccgccat cggttaccgg tatcaccagg
     9841 atacatatga aaaaggcaac gggttgaaga tcaggcagaa tgagatttat tcgcaggcgg
     9901 cctacggtgc tgacaatgcc tgggaaatat atgcccggct ggggattgcc gatctgaaaa
     9961 taggcgacgt tttcagagaa agcgaagagg tctttgcaac tctgggcgga aaggtttttt
    10021 acccggtcag cgggaagctg ggagtcggcg catttgtcca ggggacctat tatttgaatg
    10081 atttttccga taccgttgag cgcaggaagc atgttcaact gaaggatctc tgggatgttc
    10141 atttcggtgt cgccttgcag gcgacgctgc cctgcggctc gacgatgtat gcggggccgt
    10201 atttatatta ctcggaagga acgctgtctc tttcctccaa tgtgtcggac tggggatatg
    10261 ctgccggtcg ttattctgtt cagaacacgt ccattgccgg aggttttgcg ggtctggata
    10321 tcccgcttgc caaaggtttt cgtctgaacg ttgaaggaca atatgcggac aggttctccg
    10381 ccggcgccgc catcagctat acatattaag attattcctg cacattataa gtcaacccgc
    10441 agcccgtcag acaaccggtg agtcggatga atcagcagaa agaaaaattt ttccaggtac
    10501 aggatcttac caaagtttat caaatgggcg aggtggccgt tcatgccttg cgcggggtga
    10561 ccatggaact ctatgccggc gaactggttg ttcttctcgg tccgtccgga agcggcaaat
    10621 caaccctgct gaacatcatg ggcggcctcg atacagccac gggcggtgtg gtgtcctaca
    10681 gaggaaaaga actgaccaaa gccacggaaa aggaactgac cgagtaccgc cgttttcatg
    10741 tcggatttgt ttttcaattc tacaatctga tttccagcct gacggcccgg gaaaatgttt
    10801 cagttgttac ggagattgct cccgatccca tggcgcccca gcaagcgctt gacctggtcg
    10861 gcttgtctga caggttgaac cattttcctg cgcagctttc cggcggcgag cagcagcgcg
    10921 tggccatcgc ccgggccatt tccaaaaaac cggctgtgct gttgtgcgac gaaccgactg
    10981 gcgcgctcga ttcccaaaca ggcattgtgg tgctccaggc cctggagcgt atcaaccgcg
    11041 aactgggcac ggctaccgcc atcattaccc ataacgtgga tatcgcgggc atggccgacc
    11101 gcgtcattca tttaagcaac ggccatattg ccagtgttga aatcaacaaa gtgaagaaat
    11161 ccgccagcga gctgcagtgg taagatgatg aaaacgctga accgaaaact ctggcgggaa
    11221 ttgtggctga tgaaggggca ggtgtttgcc attacgctgg tggtgatgag cggcgtagcc
    11281 accttcatca tgtttatcag caccatgcat tccctcgatt ttacccgcag caagttttac
    11341 cgcgaataca attttgccga tgttttcgtc aatttaaagc gggcgccgga aaccctcaaa
    11401 tataagatca atgacattca aggcgtccat caggtggaaa ccagggtttg cgcccaggcc
    11461 aagctggata tccggggatt caaggaatcg gtgactgcca ggataacctc cattcccgat
    11521 gacggtgatc ctctgctgaa tcgtctttac atccgtaaag gacgtcttcc cgatcccgca
    11581 cgggataatg aagtggccat gaacgaaacc ttcgcgcagg cgcatggcct gaatccgggc
    11641 gataagtttg cggcggtcat taacgggaaa tggaagagac tgaccgtcac cggcattgtt
    11701 ctttctccgg aattcatttt gctgatgaag acgggcgcca tgacgccgga tttcaaacgg
    11761 tacggcgcat tatggatgac ccgcaaggct atcagcaagg cctacgatat ggaaggcgcg
    11821 ttcaacgatg ttgttttaac cgtttatccg ggaacggcat tgagtgatgt tgtgcgggat
    11881 ctggacaaca ttctgggacg gtacggcggc tttggcgctt acggacgcaa ggatcagatt
    11941 tcccaccggc tgttgagtga ggaattccgg cagctcaaga ccagttccag aatttacccc
    12001 acgatattca tctgtgtcgc cgcctttctg ctgaacattg tcatgagcag aaccatcagc
    12061 acgcagcgcg agcagatcgg cacgctcaaa gcattcggct ataataatct cagcattgcc
    12121 gttcattacg cgacgatggt gatgatgatt attgccacag gcgtgttgct ggggattgcc
    12181 ggcggtatct ggttcggcaa aatgctgggg gcgctgtaca tggaaaccta ccggttcccg
    12241 ttttttcatt atatcctcca tccgcaagtg atgatagcgg tcgtcgtgat cagtgttgcc
    12301 gctgcgctca gcgggacgat gcattcgttg tggaatgccg cccggcagac tccggcagaa
    12361 gcgatgcgac cggaagcgcc ggtgaaatac agggtatcgc tgattgagaa aactggcatc
    12421 ggccgctggc tgtcgcagcc gtcgcgtatt atcttccgca acattgaacg aaagccggtc
    12481 aagaccgtgc tggcgatcat cggcatcgca accgcctgcg cgacgatgat ctgcagcggt
    12541 tttttccggg actgtattga ttacatggtg cacattcaat tcgtcctgtc gcagaaggaa
    12601 gatatgaccg taagctttgt cgaaccgacg tcctaccggg cggtatatga actgaaacgc
    12661 atggaaggta tcagcagcgg cgaagcattc cggagtgttc cggtgcggat gaaattcggt
    12721 catcgcagtt ataaaaccgt catcaacggc atccagccgg acagccacct gaacattttg
    12781 ctggacaacc atctgaagag tatttccatg ccgccgtccg gtattctgtt gacggattat
    12841 ctgggagaga ttctcggagt gaaagcggga gatctgctga cggtcgaggc gctggaaggg
    12901 tcccgggccg tccgccaggt tccggttgtt gctatggcta aacagtatct gggggtgatg
    12961 gggtacatga acctggaagc gctcaaccgc ctgatcaacg aaggcgatgc catctccggc
    13021 gcctatctgg taacggatga gacccacaga gaaaaacttt tccagttgtt tgtgcaaatg
    13081 ccacgggtag ccgaaatcgt ggtccggcaa aacgagattc gcagcattta cgaaatcatg
    13141 gccaaggcga tgctcttctt tatgtttatt gcaacagtga tggcctgttc cattgctttc
    13201 ggtgtggtgt acaacagcgc cagaatttcc ctggcggaac gcagccgcga gctgtcttct
    13261 ctgcgcgtgc tgggatatac ccggggcgag attgcctata ttcttctggg tgaactgggg
    13321 tttataactc ttgttgccat tcccttggga tttattatag gatactggat atgcgcttat
    13381 gtggctcacg ctctgacaac cgatctatac cgcataccga tgatgatcga gcttaaaacg
    13441 tatgcgcagg cggcgttggt ggttatcgta tcggcggtga tatccggttg tatcgtccgt
    13501 cacaagctca acaacctcga tcttgtcgag gtgctcaaaa cccgggaata aaataaaaaa
    13561 actcagatgg gtcagaggat catttcatga atcacgtgtt acgcagaaaa atattcattc
    13621 tcagtatcat ggcggtcgtt gccggcctgc tcatttacgg ttttcttccc aagacgcggg
    13681 atgtggatat tgtttttgtc acgcgagggc cgcttcaggt taccattgaa gaagaaggcc
    13741 gcacccgcct gaaggaccgc tttaccattt ctgcgcccac tgccggatac ctgcagcgca
    13801 ttcatgccaa agtgggcgac aatgtgcaac agggccagat tgttgcagta ctcgaacccc
    13861 tgcagtcgca ggcgctcgat ccccgcagcc acgccgcagc acgtgcggcg gtttccgccg
    13921 ccgaggcggc tttgaaggca gccgtggaac gcgaaaaagt ggccgcggcg gatgccgcgt
    13981 atctggatca gcgcagggaa agattaaaag ccttgtatga caagggctcc attgccagag
    14041 atcagtttga ccagattcat gccgaagcgc aaaaagcgcg cgccctgcaa ttatcgtctg
    14101 cagcggaggt ccatagggca ggttccgaac tggaacaagc cagagtggcc ttgcggaatt
    14161 tttcagccgt aagaagccag ggcaatgccg ttgccgtagc ttcgccggtg aacggagccg
    14221 tgtttagaat ttatcatgaa agcgaaggtg cggtgaacat cggcgcgccg ttgctggatc
    14281 tcggcgaccc gaaaaatctt gaagtccggg tggaagtcct ttcgtcggat gcggtgaaaa
    14341 tcagacaagg aacacacgtg ctgtttaaac gctggggtaa tgatgagccg ctgacgggca
    14401 gcgtgcgtct ggtggagccg gcgggattta cgaagatatc gagccttggc gttgaggaac
    14461 agcgggttct ggtaattgtc gatattacgt cgccgccgga acagtggagc gttctcggtg
    14521 acggtttccg gatggaagcg cactttacaa tctgggaaga tgacaacgtt ctgcagattc
    14581 cggcaagcgc ccttttccgt gcgggaaaag actgggcggt tttcgttgag cacgatggaa
    14641 aagcacgcaa acgcgttatc gagatcggtc agagaaacgg actgacggct cagatcattt
    14701 ctggtttaaa agaaaacgaa aaagtgatag tatatcctga cgatacgatc agcgaaggca
    14761 caaagatacg acagagaaaa taaactaaaa cacttttgcg aggggagata tgaaagtttt
    14821 tatcattgtc ggcatgccgg cagcgggtaa gaatatcgcc cggatttatg caggatcacg
    14881 ggacatcgta tattactcga ccggcgatat tgtccgggcc gaggtcaaag cacgcggatt
    14941 ggaagtcgat gccgcaacaa catccgaggt gtccgatgaa ctgcggggtg aagatggcct
    15001 gggcgtcacc cggcttgccc tggaagaaat tctgaagacc ggcgccgatg ttggttttct
    15061 ggaaggcatg cgttcctggc aggaaataga actcattcgc gaaaaaacgg atggtgtggt
    15121 cgttgcggtc ctgactccca gaggactgcg gctggagcgc gtctgttcgc gaggacgaga
    15181 tgatgattct cctctgggct ttaacgagcg ggaccagcgt gaaattgctt atggaacggc
    15241 tattcccatt gccctcgccg atgcatatat cctaaatacc ggcacgatgg atgatgcaat
    15301 ggaacaactg gaggccattg tccagagaga gatgactccc tcatgaaatc ataaaagatt
    15361 aattcgtccc ggttcagcgg cgtgaagaag cgtacagaaa aagaaccgta tttaaacgtt
    15421 atcatgtatc gaagtcgctg aatgaaccgt tatgcccttc ccgggatttc ccaaaaaaga
    15481 ctgacagttt cattgattcc cgttctccga cgccgagaga tgtctgcggc tattcgatcg
    15541 tccgggttcc gattttgcga tgaacaaagc actgcttaaa aagtgcgggt caagatttcc
    15601 gaaatacaac acattacgtc ttgccccgaa cctggcgaac gagcagacaa ttaacaaggt
    15661 gatgaagctt aaagaagcaa ggcaaatctt cacaaacagg atgccgctta cagaagatat
    15721 tccaaagact tttttaaatt attcgattat cgaggacaaa tccggaatca tcattatgct
    15781 gttaaatcac tatgcttaat ccgtcagacg cagcaaacgt gtgcggaaat atcctttttg
    15841 cctgcatctg cgcttttgct cttttggccc ttgttgtata taaggcggca tcgaaatgcg
    15901 tcagggcgag cctcttcgct tgtgatttaa cggccagcct ggcggcgagt tgcgggttca
    15961 tgtggggcca ggtcttggac tgttgaccgc tgagaaatgc gcattccgag atcagcaaat
    16021 cggcgtttac cgaaagttcc tcggagtttt tacagtatcc ggtgtcggtg cagtaactga
    16081 tgactttgcc ctccatctcc agccggtacc cgaaacatct ggaggcgtgc tcaagcggca
    16141 gcgcccggaa tcgaaaagga cgcctgtgcc agccttcatt gatatcagtt acgttacagg
    16201 gataaggcaa ccgggtgacc ggcagagaat attttggaga caaaaattcc ccgatcgttt
    16261 ttttggtgcc ggtctgaccg ataatctcca aggatgtgaa tctgaatttc gccaggatgt
    16321 gcagaccctc catgtgatcg atgtggaaat ggctgagcag caagtaaacc ggcagatttt
    16381 tcttgatgta tttgtccgcc ttatagatgc cgtttccggc gtccagaaga atataggccg
    16441 tttttgtttc gatcagcgtg caaatggtat tgccgcaatc cgtatcatac cagccgttgg
    16501 ttcctaaaaa tgtaatcttc atcttgcccc cgtattgtta cttataagat tacgaaatac
    16561 ttttcaaatt ataaaatcgt ttttaatgag aatgaaaaaa tcttgtagag attcattgaa
    16621 gtcattcgaa atcaccggaa aagacgaact ctggcttgac aagatatcgc ggcgtgtgtt
    16681 cattatgaaa acgtttgttt caaaacagtg gaatcaggag gtgttttagt tttgacaaat
    16741 gacatcataa cactcaacga gtttgacggg acggcactgc tcaagaaggg cggaatccct
    16801 gttatggata gtattttagc cagggatgat aaggaaattg acgttgcggc caatcaactg
    16861 ggtttccccg tagcgctcaa gatttgttcc gagaagatac ttcacaagag cgaccggggt
    16921 ggcgttgcgc tgaacattgc ggatacctcc tcgctggccg atgctttccg ggacatgaat
    16981 aatagatttt ccgatgttcc tcatgcattc ctggtgcaga agatggcgcg gccggggact
    17041 gaactcatcc tgggggcaag gcgtgatccc gtgttcgggc cggtgatcct cgcgggaatc
    17101 ggcggcatct ttaccgaaat tttccgcgac accgccatcg atcttgcccc ggtggacaat
    17161 acaattgcgt gcgcgatgct cgggcgcctc agaggagcag cgctgctcga cggctaccgc
    17221 tctcagaagg ctctggatgt tgttgccatc gccgaagccc tctcatccct ctcccgatta
    17281 atatccgatc gaccggacat tctggaaata gacgtaaatc ctttcatcgc gtatccggat
    17341 ggtgccgtgg ctgtagatgc tctcgtgcgg ctgggtggca gccatgccag atccgcccgc
    17401 aaacatcccg agcctgctac ggtcgttccc tttttcaatc cggcctccat agcgattatc
    17461 ggtgcctctc gaagtccggg caagggcggc aacattattc tgcgcaacct gcaaaaggcc
    17521 ggtttcgggg gcgccattta tccgatcaat cccacgatga aggaaatcct gggtctgccc
    17581 tcttacgcaa aagtctgcga tgtgccggcg ccggtggatt tggccatgat tgtgattccc
    17641 aaaactgcgg tgccggaggc tttggaagac tgcaaggcga agagagtcac caacatcatt
    17701 ctgagtacgg gcgggtattc ggatgcaggc aaagccggcg ccgaggaaca aaaaattatt
    17761 atcgaacagg cccgcaaagc ggatatccgg atcatgggtc ccaacagcat cgggacgctt
    17821 aatccctccg ccggcatggc gacgtccatc gttggccttg aaccaatcaa ggcaggcggc
    17881 gtatcgatca tcggccagtc gggggttttt tcgtcaggct gggcgcgctg gatagcagac
    17941 accaagccct tcgggctggc caaggtggct tgcatcggca ataagggaga cattaacgaa
    18001 agcgatcttc tggaatacct ggccgaggat tgggtaaccc tgaccgtcgg catgtacctg
    18061 gaaggcgtga ttgacggacg gcgtttcatc agagccgccg aaaaagccag caggaaaaaa
    18121 ccggttgtcg tgatgaaagc aggacgcagt gaagccggag cggcggctgt tgcgtcgcat
    18181 acaggctcgc ttgcgggatc ggatgcggtg ttcgacgctg tctgccgcag aacgggtctc
    18241 gtgcgaacac acaatcccga agcatttttc gataccttat ccgcctttga gaaactcccc
    18301 ctgcctcggg gaaaccgcat gggtgtgttt tccatcacgg gcatgggttg cgtcgctgcc
    18361 acggatgccg cggaggaata cggcattgtt ctgcccgaac tgaatcccgc gacgctcaaa
    18421 aaactggcag aagtgattcc tgcatgggcg ccggtgcgca atcccattga tacctggtcg
    18481 gccatcgaac agcatggttc gaaaaagaca tcgatccata ttgcccaatg catgctggat
    18541 caaagggata tggatgccct gttgattatt tttgttctta tgccggagag catgttcgac
    18601 attccggaag cgtttgccga actattccgc aatcatccgg acaagccggt ctttgccagt
    18661 tactatgggg gcaccgccag ggaaattgct catgtccatg aaggattttc caggctgggc
    18721 gttccctgtt atcccacacc ggagcgcgcc atgtttgcgt ttggccgcat ggtggaatac
    18781 gcgcggcacc gcggtagaat ccgcacccgg ccttaaattg atgaatggca attgaggacg
    18841 gcttccttga tgaatccgtg tcttaaattc actcgtcgca gcggcgtcgc ccatgacgac
    18901 gccgtcggta atagatctgt ccttggactt gccggaactc acggccttgc gtttgagcag
    18961 ggttgctgat ccgcacccgg tccgcttttg ttttgttcgt aaatctatga agaatccata
    19021 tcgaaccaca atccatttct ttaaaataat tacatttttt atttgacaaa acctcaaaaa
    19081 agtctcatac agtacgaaat ccaaaaacac atattatctg attttttccg cacgcgaaat
    19141 ggtctgtaca ggtgctgtac ggcggggctt gaatcgtgaa ttaaaaaact tattttgaca
    19201 agttttttat aaatccaaaa gagggggaaa caggaatgaa gcaggtggct tggtctaaaa
    19261 gcagacgtcc aaaaagtaaa cccatttttt acaaaaacct tttgattatg atggcgatcg
    19321 ccgttttggg aatcgccatg gtgccggtga ccggccaggc cgtatccaga gttcccctga
    19381 atgccagcgg catctacagc gccacggcgg atgacggcag caagatattt ttgtatcgct
    19441 atgcgccgta cacgaccggt accccgaagt ttaacaccag cgctacgccg gttctgatct
    19501 ttaccggcat caccatgaat atgaaccagt acctttcctg tacgcctccc gataagaagg
    19561 atgtctacag cagcgtttat gttcctccgg ttgatcaggc tcctgaatgg gcgttgaatg
    19621 aggacaaaac agactatgaa ccgtacatca aagccgacaa aatgcgttat tacagtctgg
    19681 cgcactacct ctggcttaag gggtatgatc cctggtttgt caattatcga ggcacaggcc
    19741 gcggtgaggt aaggagcgac ggaaccaacg ccaacagcct cacgaccctt gatacctggg
    19801 ccacgcagga tgctcccgca gcaatcgcca aggtaaaatc ggtgacaggc aaacgcatgt
    19861 ttatcggcgg acatagcacg ggaggtcttg tttcctatga ttatctgcag ggcgcctata
    19921 tggaatccgg cattgcttcc tcggcctggg caaagaaagc ttactataaa ttatgctatt
    19981 ccctcggcta tatgccgcat gtcagagcca gcgctgatct tgccaaaaaa aggaatgagg
    20041 atgtaaaagg ctttatcggt attgaccccg caggcgtgcc gtcgctgccg aaccttctgg
    20101 acacccctct tttctggaca attgtaggat cgagattgta tctgccgctg gattaccttt
    20161 cagataattt aattcaggtg cttccgagca agccgctggt cggtttgctg gagggcatgt
    20221 tcgggctgct gaacaaggcg gccgccggtg attctgcgat aagcgacttc ttcgactatc
    20281 tgaatttctg ggtggtcgag gatatggacc cctgcctgga ggattggacg gtgcgttatg
    20341 ctgtcggcag cgctgccata cgcggcttcg gtcattacat ggacatgggc ctgaacaata
    20401 cccttcgtga acactactta aacggaaaag aaaactatct atcgtcgaaa ttggtcaaag
    20461 gggtttcggc acccaacccc ggccgagacg gttactacta ctatagcgag aatatggcac
    20521 gcatgaccgt accgatgatc gttttttcat cgagcacggg atccctggtg agtccgcagg
    20581 cgacttacga ctttatcata tcgaaaaaaa cgccaacggc gtatgacgag tggtatgttt
    20641 tggacggaac cggccatttc gatttggcga tgggcaagaa aatgccgacc gaaatgttcc
    20701 cgcggctggg agcgtggctg gacacagttg acgcattata aaaggcaact gcggcataga
    20761 tacatcggcc agcagaattt ttaaatcact tcaaaccggg aggatgcctg ccaggcgggc
    20821 atcctcccga aacaatgacg ctttttgaaa acccccgttc aatcatttgt tggactatcc
    20881 aatgcacctg cgaacattgc gcgatcggac cctcaacaga aaaaaaatcc aaaaaaagtg
    20941 ggtgcctttg ataaaaaagc acacaaatta aatctctgga ggaagggatt atggaacaga
    21001 cgttcacgat tacagttaat gttcaggagt tgttcatgga gtttatcccc aagttggccg
    21061 gacaatacat tcagatgcgg ggagaccagg aggaattgaa agggacagaa ctgaaaataa
    21121 ccctggacat atccggcagc gtctacagtt atatcattca ggatggcgtc ggtttcagca
    21181 tccgggaagg cgctgtcgac aatccgcacg ttcggatcgc cttttctccc gataccctgt
    21241 ccaaaatggc ggacatgaag aatattgata tgttgatcgc catgcagaaa cagctcacca
    21301 ggaaaaaatt tgatgtgttg accgcattaa gcgggaaggg tgtttttcag atcaaaaatg
    21361 ccgacgatac cgtttccgaa ataaccgcca tattcaacgg cgcagaaaag ccgatcgccg
    21421 ttttgcggct ctccatgcag gatgccaaca tgatcagcgg cgggaaagaa tctcccatta
    21481 atcttttcat gtccggtaaa atgaaaatcg aaggcgacat tgcttttgcc atgaagctgc
    21541 agccgttgtt tgttgcttga aaaccggggc ccggttaaaa aattttacat tggacgtttt
    21601 tcaatatggc gattcaatgc gtctgaatgc ttatgcccat aaaaagaatc ttttttaaaa
    21661 cccggcttgc tttcctgatg tacggttttg cctgcatgat gcggctggct gttcttatca
    21721 gtaaagattt tcgtgcgcat ctggcaactc acgactattc atttatcatg ggctccccgg
    21781 aagaggatac ttatcgctat tttcaattta tccgcggcag ggtgcaatcg gtccggggtc
    21841 gccggccatc ggattttgcc ctgatctggc gggataatcg atccggcggt aaagtcatga
    21901 tcgatttggc agcgggcaaa cgcaaggcgc tgaaccgcgc cgtcgtgcat ggtttactga
    21961 ccctggaagg agaaggaaag tacatttcgc tgttgattga aaccatgaat cgtcttaacc
    22021 gtttgttcag acatgagcgg aaaacgaaca atcgtgtcaa agccggttga ttcgtaaaag
    22081 caggcataaa ataaagcaaa taaacggatt tctgataatc tgaaaaagac gttcttcggg
    22141 gcgaaatata tcaccaatat attgtttata ttagcaaaaa tcaacccccc tgttttttgt
    22201 ccaaccttgc tcccgccgac atttcctgtt ttttcttgca atgtttatag gaagtcagta
    22261 ttattaatta tgatcatgcg atgatcataa gagttaaaca tgaatgaaaa cttgtatttc
    22321 aagacctgac cttatctgtt tgtattctat cagttcccct tctagctttg tcagtactgc
    22381 agttttcaaa tcaaaacgtg aaattacctg agccggctga tggtgttcgg atcgtccgga
    22441 cctgaagccg ggacagacat aaaatacagg agagatatta tgagtgaatt taaaccttac
    22501 aacgacacaa caatcccagg cattttcaga aatcaggtgg agaaatcccg cgacataatt
    22561 tttctgagaa tacgcgatga aaacaaaaag tggaaagagt acacgtacgg cgacatcagc
    22621 gggcaggtcg atgcacttgc ttcgtacttt ctgaaaaaag gcatccagcc gacagataaa
    22681 atcgccattt atgccaacaa ccggcctgaa tgggccgtag ccgatctcgc cacgctggcc
    22741 atcggagcag ccgacgtcac cgtctatccg accaactccg ggccggaagc cgaatacatc
    22801 ctgaatgatt cccgggcaaa aatatgtatg tgcgccggga aattccaggt ggacaatctg
    22861 ttgaaggaaa aaaataatct tcattatctg caggaaatca tcgttttcga cgaaattgat
    22921 tccaggtatc cgatcgtcgt gaagttttcc agtgtcttgc agatcggtaa agccagcctc
    22981 aatctcgacg aaatcgacaa acggttgaga aatatcgacg ttcaggaaac ggcaacccta
    23041 atttatacct cgggcacgac gggcaatccc aaaggcgcca tgctgaccca cagcaatctg
    23101 gtatccaacg taaaacaatt cgtcgagcat catccgttcc cccccagggg aacgtgtgtg
    23161 tcgcttcttc cgctttccca tgcgttggag cgcacagtcg gctacaattc cgccatgcat
    23221 gaagggtgca ccatctccta caccaaaggt gccgagaccc tgctgggcga cttgaaagac
    23281 gtcaggccga cgctggccgt atacgtgccg cgtgtcctgg aaaaaattta tgaaggcgtc
    23341 atgagccagc tgggaaacgc tccaccgagt aaacagaagc tgtttcacgc atcgatgggc
    23401 gtcggtaaaa aggcgctgcc ttatattctt tacaacaaac cggtgccgtt tttcctgaat
    23461 ctgcaataca agcttttcga aaaactcatt tattccaagt tgcgcaaggc cctggggctt
    23521 gatcgcatgg tgacgatcgg ggttggtggc gcgccgttat ccaacaccat caatgagttc
    23581 tttcagatca ttggcgttga aatccatctg ggttacggac tgaccgaaac cacgccgatc
    23641 acgcatctga acacatacaa gcatctcaag ccgatcaaga taggcacctg cggtccggcc
    23701 tttccgcgca cggaatgcaa gattgcagaa gacggcgaaa tcctgatccg cggacctcag
    23761 gtgatgaaag gatattacaa ccggccggaa gacacagcgc aggtgctcac gaaagacgga
    23821 tggttttaca ccggcgacat cggcaccatc gacgaagacg gttatctgac gatcaccgat
    23881 cgcaaaaaag atattttgat taccgcgggc ggcaaaaatg tgtcacctca ggtcatcgag
    23941 ggcgcatttg tcatgcatcc gctcatcgag cagatcgcca ttatgggcga ccagaaaaaa
    24001 tacctcgttg ccctgatcgt gccctgtttc accgaactga ttaaatgggc caaatcgaaa
    24061 aatatcaacg agaccgatcc gacaaaactg atcgcctatc cggaggttgt ccagaaatac
    24121 aaggagacca tcgatgaatt gaacaaaccg ctgggtcgcg tggaacagat caaacgattt
    24181 tctctcatta accacgcatt cagccaggaa agcggcgaac tgacgcctac gctcaagatc
    24241 aagcgcaaga tcgttctggc taattaccaa gaccgtatcg aggagatgta taaagaatag
    24301 cctaaaaaat tgtgtgaaca atgagggggt aattcgggac agcaattccg aattacccct
    24361 tttgcatctt ttttgttata cttcacacag acgtcatgga aggaggaatg aatatgcatt
    24421 acagaatctg taaaacggtc acaatcgtat tattcacgat gctggcaatg ttctgtctgt
    24481 cgtgcaccga agcgaaatgc aagatcgatc agacagtatg caattatgat tgtccgtcaa
    24541 ccatcggagt gaaacaggcc tgcgagcaga aatgcaatct gttatatgat atttgcagaa
    24601 gtcagaaatg atattccttg atctgaagtt caatgtttgc aagatgccac cacgccggga
    24661 ggtcagtcgt cacgtccgtt catcaataat gaagtaatca ggctgaatcg tcttgctaaa
    24721 attccgtggc tagtgtaaga ctttccgcca acttgttatt tatattataa aaaaggctca
    24781 atgaccttct tggtgttgaa tgtctgcacc ctgagccggt tgatggagac attgcaagat
    24841 taaatggcag tgtaaataaa gtaagagaga aggctatgac gacaccgata cagcagaaga
    24901 tccgagataa aaaaagaaca gccaagcaga ttgccgaaat ggtcaagtcc ggcgattgga
    24961 ttaatcacgg cgcggtcggc ggtgattcca ctgcctgcac cgaagcggtc gccaagcgtc
    25021 tgggcggagg gaaaagcgat cttcacgaca tcgagttctg gcttgccggc aacttttatc
    25081 cgcatccgga attgcaggag atcgatccga tgcagcagta ccactgcgtg catgaacatt
    25141 tctttttccc ctggaaccgc aaggcgcggg aggataacga cgtaaccagc tggacgccgt
    25201 ggggctgggc gggcggcatc tgggcgcatc attaccggtt cgccaacggc gagcggaaaa
    25261 accgcggtat cgactggtgg tttaacgcgg cgaccgcacc ggacaatcac ggttatttca
    25321 acatgtccta tgggaccaac agcagcaaca tcttcaagca gacggcaaag aaaatcgttt
    25381 ttgaagtcag agccgattac ccctgggcga aaggttgccg tttcaatacc attcacattg
    25441 acgatatcga ttactgggtg gaggtggact gcgaaaaata caagtggccg cagatgaatg
    25501 aaaagggcat tgtcgcaagg ccgatggaac aggaaatcgc cagccacatc atgaccatca
    25561 tgcgcgaccg cgatgtcgtg caactgggca tcggatcgct tccctcggcg tgcgtggccg
    25621 ccatggcgga cgcgggtttc aaatatctgg gcatccatac ggaaatgctc aattacggtc
    25681 tgattaaaat gatcgaatcg gggcaggtga ccaacgcgta caagaggctg gatcagggca
    25741 agagcgtctg gacgttcgct tttcccgtgg atgtgaagtg gtattacgaa accgtacacc
    25801 gcaatcaagc gctggcggtt tacgatatcg attacaccaa taatctcgat gtgctgcgtc
    25861 gcgtcgacaa catgatcgcc atcaacaaca ccgttgccgt gaatttgctg ggtcaaatga
    25921 cctgttcgtt ttacggcaag cgtcccattt ccggcacggg tggatttttc cagttcatca
    25981 tttttgcagc ccagtccaaa ggcggtcgca gtgttgcggc catgacgtcg tgcagcaaac
    26041 acaacacgcc gcgcattgtt cccttcctgc cggaaggatc ggtggtggac gtgcccgcac
    26101 agtttgccca atacgtctgc acggaatacg gcatggtcaa tcttcggggc ctcaacggct
    26161 acgaacgcgc ccgcgcgctg atttcaatcg cgcatcccga cgaccgcgcg tggctggaga
    26221 aggaagcccg gcggcacggc ctgatgccac cgcagtttcc cgcgaatttc ctgccggaag
    26281 aaggcggcaa ccggcgctat ccgtcttatt cggatcggcg cagttacaaa atgcccatgg
    26341 gcagttccat ctggggttac gactgggatc ccattcaatc gggcaaataa aaaaagagac
    26401 aaaagtacag cggcccctgt gttcaatttg taacgaggaa ccggttgtcc ttttcaaagg
    26461 tgttaaaata tgaaagttat tcccgccgat gcttcgaccg tcgtgctcct acgttcctgt
    26521 ccggatgctt cggtaagagg cattgaagtt ctgatggttt tacgaaaccg caaaagcagt
    26581 tttgtgccgg ggtattatgt tttccccggc ggaggtctgg aaaatgatga ttttgccgac
    26641 ggcatggaac gtttcattgt gggcatggat cgtcttgccg cgtccaaaat cctgtccgac
    26701 atgcgttacc cggagcaggc gctcggggca tgggtggccg gtatccgcga aacatttgaa
    26761 gaggtgggca ttttgctggc gcagaaaaaa gacggttcac ccgtgacgat tagagcggaa
    26821 aatgaacggc agcgcttttg cgatcaccgc aaagcgctca tcagcggtaa aatgcgtttt
    26881 attgaaatgc tggcaaccga agagcttctt ctgcctctgg acagacttta ttatttttcg
    26941 cactggatta caccggaacc ttttccccaa cgctatgatg ttcgtttttt tgtgacggaa
    27001 attccgtccg atcaggccgt cattcatgac ggcgtcgaat tgaccgatca tgtctgggtt
    27061 cgtcccgctg aggcgataag gcaatatgag cagggccggc tggatatggt tttgccgcag
    27121 atcatgaccc tcgagaatat caaaaattgc aaaacggtgg cggaagtgat tgcgcgcgtc
    27181 cggaaacgca atgtaccggc catactgact aaaatcaaac ggatcgacgg caaagacgtg
    27241 gaagttatgc cggacggagc ggtttttgag aatcgtccgc ccgcgtattc ctggcccgat
    27301 aagagcgatt aattttccgg cattgcgcct gcgaaatatc ccggaagtga ttcatattaa
    27361 atattaataa cattttttat tcgttttcgc tcaggcagtt atacctttcc cattttttgc
    27421 actcgtaata atgaatttgt ttggtgtttt ccggataacg tccgcaattg aaataacgcc
    27481 agaaaaaaga accataactt ttgcattgat tgtgtttcat atcatgaaga ggacacgctc
    27541 ttcttgtgca gcaattgttg cacaacccgc aacctttatc actatgctgc cacagtgcgc
    27601 ggacacgaac acgtttggga tcgggagcat tgattggtgg atctgtccat gggatgtaaa
    27661 acatttcccg gtaatccgga ttatagagca gttcgacaac ctgacgccaa aaagcaaaaa
    27721 caaggcggat gatatagtga tgatatttta taaaatttag gtctttaaaa aagtaccatg
    27781 agaacagagg acacaaaaca tagcctagca gaccgcccga cagcgcagtt cccattgccg
    27841 ctgttaaaaa aataaatctt gccataaacc attttgtttt ttgcatcatc gctattttga
    27901 tttttgcgtt aattgattaa gctcccgcat cgacgcaaga atttttcgca tgtccggttt
    27961 tgcggcaaga ttgatagatt ctgccttttg tgtatccagg tcgaaaagca tttccttgcc
    28021 gcctttccgt ttttgcaaaa acttaaaacg ttgtccgccg atatcagcca cgacgcctga
    28081 aacgtcataa caggcggtgt aaaccgcctt gcgcggcgct gcaagttgaa aaggaatgcc
    28141 cgaccaatct tttggtattg cggcgccaat tgcatataac atggtagggg caacatcaac
    28201 ttggctggac agcgagcgtt caggatagtt ggattgcagt ccatcataga ctagcatggg
    28261 gatacgcacc atgggttcga aaggctcgaa accatgaccg aaattatcga attctccaag
    28321 gaactcacca tgatcggcag taatgacgat taatgcgcgt tgtaacatac cttcccgatc
    28381 cagaatctcg aaaatttgtc tgatcgtgtt gtcagcctga agaacaccat tatgataatt
    28441 gttttgatac gcgcgctgcg aataaaatag ttttggaata tcccattctt tgtcaggcag
    28501 ccattttttg aaacgcggat cgcgcaaacc gatgatgtgt gcggacatca gatgaatata
    28561 aagaaacgta cggtccgccg atttccagtt gtatgtttgc aaccagccta aaacggatcg
    28621 atcatcgttt gcatattttt tcggttcaat ggatccgtcg caaaaaagat caatatggga
    28681 acccaagacc tggcgtaaac cgaaaaaatt cgtcgtgtcg ccgcttaaaa gaaagctgtt
    28741 acgataattg tactttttga gcacatcggc cagattcaca gggggctcgt ttaactggtg
    28801 ccagtatttg gacgacaata tgcctatcat gccgcaaaac gacgtggtgc aaattgaata
    28861 tgcattatcg attcgacgaa gttttcctcc gcgccataag tgcgagagga aaggcgtggt
    28921 ttcccgggga gccccataca cacccatttg atcgctgcgc aatgcatcga ctgtgataag
    28981 cacaagcggc cggggagtta ttccttcata cggctccgct gatgttgttg actgtttttc
    29041 tccggacaaa ggacgtttgt tgaatattcc ctgcggagct tgtctcaaac aaccatgccc
    29101 gttaagccag gcgatgtgca aaggttcgct catttgccag cttttgtaac tgtttgaata
    29161 cacgattgca aatatcatca cgaggaataa gaccagaacc ggccatcggg aatgaggttg
    29221 tgttgcgtca ttttcatttg cggtaaattc ttccgccgca acaccgctgt aatagtaata
    29281 tcctgcgaat attaacagcc atataaataa gaaaataatg atcgccgctt caacagacaa
    29341 tccgtaaata ttcagcagca catcgatatg tcccgaatag gcgctcaata attcaaacgt
    29401 aaaaggaccg ctccagcttt tatagcccaa tatggtcata acgtaaaaac aaagcaggaa
    29461 ataatgaaca aagctcagaa tgagcaccag ggtaattcgc ctgacgacgg aaggaacaat
    29521 tttgaaagat tttaagtcaa acggaatcat taatacgatc aacagcaagc acacaatgat
    29581 ccaatgcatg aacaagcgca tgaaatatcc atcatatgta tagttgacat atccgataac
    29641 tgccgttgcc tgtaagaacc agaccagcgt taatttcatg acatgtttcg gtggcagcca
    29701 tttcagaaaa agaattttta gatggggatt tttcaaaagc aaccctctca atgctttgct
    29761 caacggaaaa gatcgaaatt aaattaacgt gcaaattcgt aatattttat tacatgaaaa
    29821 acaagaaaaa actgatgaga tcaatctgct ttcattatcg taattctttt ttaatgttgc
    29881 tgatgtaatc ggaaagaatc tttgacaagt cgccgttatt tttgttttcg tcaatcagac
    29941 gtataaccgc gcttccgatg acaacaccgt cggcaaaacg tccaatttct cgcgcctgtt
    30001 cgggtcggga tattccgaaa ccgacggcta tcggcaattt agttatttta cggattttgc
    30061 tcacttccgt tttgatattg ccgatctttg gcgcggctgt gcccgtgatc ccggtgatgg
    30121 aaatgtagta gagaaatcct gaagctttcg aggcaatctt ttttaatctt tctttatccg
    30181 tggtgggagc gatgagagaa ataaaatcca taccgacagc atccgtgtaa tctcggagct
    30241 cctttgcttc ttcatagggc agatcaacaa ccagcacgcc gttcacgccc gcatgccggg
    30301 cagcttgagc aaagtttttc acaccgtagg caaagatggg attgaagtaa ccgaaaagga
    30361 caatcggaat atcggaggtc ttgcgcacgt cggcaaccat tttcagcaca cctttcaaag
    30421 tcgtcccggc tttgagcgag cgttgagccg ccgcctgaat aaccggcccg tcggccgtgg
    30481 gatcggagaa aggtacgccg atttccagga tgtccactcc ggatttttcc agagcaaaga
    30541 ttagttgctt tgtaatatca agcgacggat cgcctgccgt taaataaaca atcagtgcct
    30601 tttcatttct ggcgcgtaat tccgaaaatt tattttcaat tggtttcatc agcccataac
    30661 ccccttctcg ataatcgtct gagcgtcctt gtcgccgcgc cccgacagac aaatcacaat
    30721 aattttattc ttagtcattt tgggcgccag tttcatcgcg taggcgatcg catgcgcgct
    30781 ctccagcgcg ggaataatgc cttccttcag cgacaaaaat gaaaaagcct taatcgcttc
    30841 cttatccgtt atcgttacgt atttgacttt tttcgtagca tgaaaatagg catgctcggg
    30901 acccacaccg ggataatcca ggccggcggc aatggaatag gcatcgcgca cctgtccatg
    30961 tgcgtcctga aggagatagg ttttatttcc atgcagaacg ccggctttgc cgccattgat
    31021 gctggccgaa tgttcagacg tattcatgcc atgacctgcc gcttccacgc catacatggc
    31081 aatatttttt tcattacgga aagggtagaa agtgcccatg gcgttgctgc cgccccccac
    31141 gcaggcgatc atcacgtcgg gatatcggcc ttcgaatttc atgatttgcc ttttcgtttc
    31201 tttgccgatg accgactgaa aatcgcgtac catccccgga taaggatgcg gcccggccgt
    31261 tgtgccgatc acgtaaaatg tgtcacgcac tgaagatacc cagtagcgca tggcttcgtt
    31321 catcgcgtct ttaagcgtgg cggtgccgct tttcaccggc acgacttccg cgcccagggt
    31381 cttcatccgg aaaacattgg gcgcctgccg gcggatgtcc tcttcgccca taaatatttt
    31441 gcattccatt ccgaagagcg ccgcgactgt cgccgtggcg acaccgtgct gtcccgcgcc
    31501 ggtttccgcg atgactttct tcttgcccat ccggcgcgcc agcagcgcct gccccagcgt
    31561 attgtttatc ttatgggagc cggtatggtt cagatcttcg cgcttcaggt aaattttcgc
    31621 gccgcgtaaa gattccgaca ttcttcttgc gcagtaaagt ggcgtgacgc gtccggcata
    31681 ttccctctga taccattcaa actctttctc gaagcccctg tccttttgtg cgattttgta
    31741 agccttttcc agctcgatta atgcgggcat cagcgtctcg gcagcatagc gtccgccgaa
    31801 aatgccgaaa tggccgtttt tgtcaggcaa tgtattcttc atgtgtccct ctttttaaaa
    31861 atggattgaa aattttcatg ctttgtatcg aaggcccgga tggtgtcaaa gatacgggcc
    31921 agcttctgat gatctttttt ccccggcgat gcttccactc cgctgttgat gtccaaagcg
    31981 tccggagtga ctgttttcaa agcgtcggca acattttcgg cgttgagtcc tccggataaa
    32041 atcagcggta ttttacccct gaggccagcg gccaagtccc agttggattt tttaccggtg
    32101 ccgccgtaac gtccggcatg acggttgtcc accaggatgg ccgccacagg ataggcgcag
    32161 gcgggggtta aatcaaattc gttctttaac tcaaccgcct taatcaatac ctgcgacggg
    32221 aaaagacggc aatcgtcggg cgattcgtct ccgtgcagtt gaatcatatc gagtccgcag
    32281 gtttcgacca gcttttttat atttttcggt tgttcattca cgaatactcc caccttcaca
    32341 accttatccg gcaaaacacg gataattttt tctgcatcat ccggttttat atagcggggc
    32401 gagggcggat aaaaaataaa gcccaacgca tccgcgccgc aatttactgc agcgaaagca
    32461 tctcctttac tggctatgcc gcagattttt acttgagtca cttcagctct ctcctttcaa
    32521 ctcgcgcagt ttcttgccga tgtccggcgc cgtgacgaga tgctcaccga ttaaaaaggt
    32581 cttaattccc gctttgatca ataattcaat atcttttctt ttccggatgc cgctttccgc
    32641 cacgacaagg ttgttttccg gaatccgctt tttgagtttg acgctggttt cgatatccgt
    32701 aataaatgtg tccagatcgc ggttattgat accgataatc tccgcgccgg cggccagcgc
    32761 taaatccagt tcttcctgag agtggacttc caccagttgg cttaagccaa cttccccgga
    32821 gagcgaaatg aaatcagcaa gtttttctcc taacacccgt acgataagca aaacagcgtc
    32881 agctccaatt atccgcgtct caaaaatctg gaggggatca acaataaaat ccttccgcaa
    32941 taacggcaat tgtacggttg gccttatttg cttcagataa tttttgtgtc cggaaaaata
    33001 cttttcatcg gtgagcatgg aaatcgccgc cgcaccattt tgttcataaa tccgcgcaat
    33061 cctcaaagga tcgaaatcag tgaccagccg gcctcgtgat ggtgaggcgc acttgacttc
    33121 cgcgataatg ttgcagtcgc tgctcgttaa cgcctttttg aaatcccggc agggggcaaa
    33181 tccggcaatt tcttttttca gttgcgcctc tgttgtttct tttttcagtt gcgccacttc
    33241 ttcttttttt gtttcaataa ttttgtcgag catttccacg tcgctctgct cctgggatag
    33301 tttccaatag cctttggtta ttggataatt cgatttgcga atttcagttc acaacgtttc
    33361 tggaattcga gtctttttac gttcagcaag tttcagtgtg cctatagtga ccttcaggtt
    33421 actttgcgcc tgggatagtt ccgctaacgc ttcgcgcgtc attgcattcg cgctcagcga
    33481 gtatcgctaa ctgttgctca tttcgatcag tccttgcagc tttttaacgg ctgcgccgcc
    33541 gtcaatgcag tcggttgcaa tcgccaaccc ctccttgatg tctttcgcct tttctccggc
    33601 aacaatcgcc agggcagcat tcagcaggac aacatgacgg cagggaccgt ttttgcctga
    33661 gagcacatcc cgggtaattt gggcattggc cgaaggatcg ccgccgcgaa tggattccag
    33721 ggaatccagc cgtccgaagt aatcaacagg attaatatta tatgtccgga caatcccgtc
    33781 acggagttcg gccacacgcg tctctccggt aatggtggct tcgtccagtc cgtccagtcc
    33841 gtgaacgaca aacgcttttt ttgtacccat gttttttaaa acatgcgcga acatttctgt
    33901 aagttttgca tcgtaaacgc caagcaactg ggctgtagct cccgccgggt tggtcagcgg
    33961 gcccagcata ttaaaaatgg ttcgaatgcc gatttcccgc cgcggaccga tggcgtattt
    34021 catcgcgccg tggagtttgg gggcaaatag aaatccgatg ccgatctgct gaacacattc
    34081 ttcaactatt tcctggtcgg cgctgatatt cacgccaagc gcttccagaa catcggcgct
    34141 gccgcagccg ctggaaacgg cccggttgcc gtgcttggcg acggtcagtc ccgccgcagc
    34201 tgcaacaaag gccgccgtcg tggaaatatt gaacgtattg agcttgtcgc cgccggtgcc
    34261 gcaggtgtcg atgatagttg aagaacaggc attaatacga gtggctttct ggcgcatgat
    34321 gcgtgctgcg cccgagactt cttccacggt ttctcccttc atgcgcagcg ctgtgatcaa
    34381 cgaggctatt tgcgcggggg tagcaactcc ctccatgatt tcatccatcg cggccatcat
    34441 ttccgcttcc ggcaaatccg ttttcatcgt ggctttttcg attgcttctt taatcatgat
    34501 cgtcgctcct tattatttcg tatatattaa aaaatttctg atgatgcgtt ttccttgcgg
    34561 tgtcagaacg gattccggat ggaactgaat gccttccacc ggatattttt tgtgccgtaa
    34621 tcccatgatc tcgccttcgt ccgtcagtgc gctgatttcc agacagtcag gcagcgtttc
    34681 cggatagaca atcagggaat gataccgccc ggcggggaaa ggattgggca gtccggcaaa
    34741 gatagtttga ccgtcgtgtt ttacatgaga tattttcccg tgcatgattc ttccggcgcg
    34801 cgatactgtg gcgccgaacg cgtaaccgat ggactgatgt cccaggcaga tgcccatgat
    34861 gggaacgtcc tgataaaatt tccgtataac ctcaaccgtt actcccgcct cggcgggcga
    34921 acagggaccg ggcgaaagaa aaatcgctct cggctgaaga cgcctgatgt catccgtttt
    34981 tattttgtca ttgcgataag tgttcacttt ttcgcccatc tgttccagat actgaacgat
    35041 gttgaatgta aaggaatcgt aattatcgat cattaaaatc atatttattt ctcctgccgg
    35101 ccgtttttaa accaaattgc tttcaaatat taagatatga atttttgaaa gcttacatgg
    35161 tcttatgcta atcatgcctg attggtactg atttcaaatc ctcctgctgc aagctttatg
    35221 gcctgcagca tcccgcgggc tttgttgagc gattcctgat gttccgattc ggggatggaa
    35281 tcatacacaa tgcccgctcc ggcctgaacg taaattctgc cgtttttcag aatcattgtt
    35341 ctgatggtaa tgcagaaatc catattgccg ctgaaactga agtaaccgac ggctccgccg
    35401 taaggtcccc gtttgacttt ctccagttcg ctgatgattt gcatcgctcg cactttcggt
    35461 gcaccggtta gggtgcctgc cggaaaggtt gctgaaagaa cgtcgaaagc atctttgcct
    35521 ttggtcaact gcgcgcgcac attggaaacc agatgcatga cgtgcgaata tttctccacc
    35581 atcatgaatt gattgacctg aacactgccg gtttgcgcca cgcgtcccag atcgttgcgt
    35641 cccagatcca ccagcatgac atgctccgca cgctctttct catcggacag cagttcatcg
    35701 gacagcgcgc ggtcttcctg ttccgttctg ccacgcggcc ttgtcccggc aatcgggcgc
    35761 aactccatgg tatcgtcctc tttgcggacc atcacctcgg gtgaagagcc aatcagcgtc
    35821 agatcgtcca tcttcaaaaa gaaaaggtac ggcgacggat tgatgaaacg cagtgcgcgg
    35881 tacaaatcaa tggagctgtt taaatattcg gtttcgaaac gctgagacag cacgacctga
    35941 atgatgtcgc ccgccgcaat atattccttg gctttttcaa tggcgtcttt atactggtga
    36001 caggtcatat tggattgaaa attgacgtca ccttctgttg ctgcggctat cttgtgcgat
    36061 gcggatgctg aaatgatttc aatcatttct tcaattttcc ggcatgatgc tgcgtagcct
    36121 gcttcaagcg attcctcatc ttcagtaaaa gtgcaggcaa caacttttat ggtgtgacgc
    36181 acgttgtcga aaatcatcag ggaatcgctg atgataaaaa cagcatcatc cagattcaga
    36241 tcatccggcg gactgggtgg aagttcttcg aaatagcgca ccatctcata acccagatac
    36301 cctaccgcgc cgccgtaaaa acgcggcagg ccgggaagcg ccaccggttt atagcggttc
    36361 agcaactcac ggagaaaatt caaaggaccg ccatgatggt ggcgggttgt cacatttcct
    36421 ttttcatcaa tgataactct atcgccctgg actctgaaaa ccacgctggc gtccgttccc
    36481 aaaaacgaat agcatcccca tttctcgccg ccctcgacgc tttccagaag gaatacatgg
    36541 tcttttttcc gaagtttcat taaaacggaa acaggcgttt ccacatcggc taggatttcc
    36601 cgatagaccg gaatcagatt tcctttttga gctaactttt caaattctgc aaaattcggt
    36661 tggtacataa aaattctccc taaaacgact aaagccgtga aatctattca cggcttcaat
    36721 ctgtagatac aaaaaagccg tgaaggtccc ggaggcactc cacggctttg actttaatta
    36781 aaaaggatca gcggcggaaa tccctccctt tgaggttcca ccaccagttg ttaagcatta
    36841 aggtttttac tgttgttttc ataaattaat ttccaatctc aatgtgggat tcctataaca
    36901 gcaagaaaat gacttgtcaa tagattttaa tgagactcgc cgaaaacgag cgaagtgaag
    36961 ttagaggcgc aagtcgaatt atccccgaag cgcagcggag agggattgtg aattttaaaa
    37021 gcataacaat ctgttagctt gcatcagaaa ggcagcggca cctggatcag ggaggggaaa
    37081 caacggcgac aggccttgtt ttgcatggaa cgggcgcggc acgcatccag atcgataaaa
    37141 taaccgtatc tttcgcacca aacattttcc cgttgcggtt ttactctggt cgttgaacct
    37201 cccgcgggtt tgagactgcg acgtattttt ttcatggcgg caaagatatg agataaaagg
    37261 gggcattgtc aacagaagat tattaatcct gcttgacacg tgtttgttta tttttatata
    37321 agttttccaa gccttgcaaa attggcgtat cgggaagaaa tttatgatgt tactggaatc
    37381 ctttttatcc gccgtgaaga gcatccgggt gcaggatgtt ctggatgtga tgatcatcgc
    37441 catcatgatc tttgctcttt tgacctggtt taaaacacgg gcgtcccgtt tcgtactcat
    37501 cggcatcata ctgttaggcg gcgtttatat cgcggcgcgc ttttttcagc tttacctcac
    37561 cactattgtt ctgcagagct tttttgctat tctgcttttt gttctggtgg tcatttttca
    37621 ggaagatctc cgtcgctttt ttgaaaggtt ggcgatgctg ggaagcctcg gcaagaaatt
    37681 tccggagatc agcggcttga aaagcacggc ggaaatcatt gcccagactg cggcaaacct
    37741 agccaagaaa aaaacaggag ctttgattgt tctgcggggc aatgaccccc tggaaaggca
    37801 catcaacgga ggaaccaaac tggatggcgt ggtcagcggg gctctgctgg aaagcatttt
    37861 tgatccgcat tccgtcggcc atgacggggc cgtcattata gaagacaaca ggatcagtca
    37921 attcggctgt catctgccgc tttccgccaa tacctcaaaa tattccaatt tgggattgcg
    37981 tcataccgcg gcactcggtc tttccgaacg atccgacgcc atatgcgttg tggtatccga
    38041 agaacgaggg accatttctc tggcctacat ggaaaatctt acggtggtgc ccagcgcggc
    38101 cgtcctgcat gaaacgctgg aacagtttta tatccgcaac acgccgacga aaaaatcgca
    38161 tcccgcgttg aactggctta aagaaaacac actggaaaaa gttattgcaa tagcgatggc
    38221 ctgtattttg tggatggcgt tcggctatca gagggacatt gtacggcggg attttattgt
    38281 tcccattgag tacaaaaata ttccgcgcaa ctggcagatc gatgaaatgc aggtcaccga
    38341 tgcccgggtc gtgctgaaag gtccggagca ggcgtttcgt ctgcttgacg aacgctcttt
    38401 gaaattatcc ttggatctat cgggtatcgc cgacaaaaaa cgcgaattca acctgagcgc
    38461 cgacatgatt aatgttccat ccaatctgac cattgcggat attgttcccg ccaaaattca
    38521 cgtaacggcc tccaaacgta ttccctggac attccctgtt catgtcaaca ctcaaaatgc
    38581 tctggcccat catctttcgc tgcagcggtt aagtgtagcg ccgtccaaag ttcgggtttt
    38641 gatcagtgca cagacggatc cggatcaaat caaaatagaa acggaaccgg ttgatctaaa
    38701 gagaatttta tccacgacaa cggttgatac aaaaatcgtg cttcccatcg gtgttgcttt
    38761 accggaggga caatcgccgg ctgcccgggt gcagattaag gtgaaacaaa aggcttcttc
    38821 ttaaatgagg cagacatgac gtccaagtat cacaaaagat ccagaaaaac gggtcttgcg
    38881 cccggaagtc tggtgcacat cggcagtccg tatgccgaaa aatcaaaaat cactctcatg
    38941 caatacaatg aaggtttttt tgaagcaaag gacctggaaa gcctgaagga tttccaggcg
    39001 gataaagata aagagatcat cacctggctg aatgttgacg gattaacgga catacaatta
    39061 ttcgaagaaa ttggcagaat tttcgggttg cacccgcttg ttctcgaaga tattttaaat
    39121 acggctcagc gtccgaaaat ggaagattac ggaggctata tttacctcgt ggccagaaat
    39181 tttcatgatc aggaaaacgg cagtttgctt ttcgagcaga tcagtatcat tctggggaaa
    39241 aatttcattc tctcttttcg ggaaaaagaa agcaatcttt tcaaatcaat ccgggaaaaa
    39301 atcagtcaga acaagggcag aatacgaaag gaaaacacag attatcttgc ccacgccctg
    39361 ctggataata ttgtcgataa ttattttgtt attctggaaa atctggagga aaaaattgaa
    39421 gctctcgaag acgatctgat cagacagccc gatagctcat ccctccaggc aattcacatg
    39481 ctcaaacgaa aactgattct gctgggcaaa tcgctgtggc ccctgcggga agctatcagc
    39541 gcactggaga ggtcggattc ggcgctgatt aacaaaacaa cggttgtcta cttcaaggat
    39601 atctatgatc acaccatcgc gatcatcgat accgtggaga cattcaagga tatgctttcc
    39661 ggtatcctgg atgtttatct ggataccgtc agcaaccggt tgaacgaagt tatgaaaatt
    39721 ctgacggtca ttgcgacaat tttcatgccc ctgacattca tcgccggaat atacggtatg
    39781 aatttcaagt acatgcccga actggacatg cgctgggggt attttggaat tttaggcgtc
    39841 atgctgcttg tcgcactgtt catggtgaga tattttagaa aaaagaactg gttttaattt
    39901 tgttgcattc aatcataaaa tatgcaaaat ttacccagaa tttatttaag aatcaggagg
    39961 caatgaagat gagtgttttt gcagcaagcg atatcgtgga agtcgctatc agaattgaag
    40021 aaaacggcgt taatttttat aaatttgccg aacagattgc caagaaagaa gacgaaaaga
    40081 agctttttgc cgggctggcg caggcggaag ttgctcataa gaaaacattt gaaaaacttt
    40141 ttgccggtat ggaaaaatcc aaccttcagg aaagttatga gggggaattc ggcgaatatc
    40201 tccgtgcgta cgttgataat aatttgatct tcaccaagga aataatggat aaacagttgg
    40261 ccaaagttac ggataccaca tctgccctgg aatttgccat tcagagggaa ctggactcca
    40321 ttctttatta tcacgaaatc aaaaaaatgg tccctgcggc acagcacgat gcgctggaga
    40381 agattatcga agaggagaga aagcattttc aggccctgac agccatgaaa aaacgtttag
    40441 cttgttagtc attcaactca actaaccata aaaaagccgc cctaaaaggc ggctttttta
    40501 tggttagcca atgaattttc acagcttttc ttcaggggaa aaagctttct tatggcgttt
    40561 tttgtttcgc ttaaaaacaa gatatcagca aaaataatca gagggctttg cccttcccct
    40621 aatccttttg aaaaatttca agaacaatac gcgcgtatga gaataaatct ttaacgcaag
    40681 gggttacaag ggcttgatag cagggcaggc atgaggatag cggctatgcc gatttatcag
    40741 tcgattcgtg cgaaatcttc tctggcagta ttttaatctg cgatatccct agctcatttg
    40801 ccaggtaaga tgccagagca taaataaata tcaacaaacc catcatttcc aatgtttcct
    40861 ctactataac aaaatataga taataatatt tatcctgtcc gtatatctcc acatggcgca
    40921 attccagcgc ttctattcct gcggcaccca gcataaaaat gataaaggaa aaaacaaaca
    40981 gatattttgt ttttttaggc aaatgaatca tgaaacgtaa ataaacgaga gaaatcggtg
    41041 caagaacaac taagtaaata agaacccatg cattagtagg gaaaagcacg atctttatat
    41101 taaacagagc taatatatga tctgaaattt cgtgaaagga aatgaattcg tcgatcaata
    41161 gaaaactgaa aattatcgcc aatcccaacc agtatctata acgggaggac tgtgtgtttc
    41221 tctcaaaaaa agcaattaag accagaaaaa tgacacaaag gaaccattcc aaaatagtgt
    41281 aaagcgtggg aatatttgcc tctccatcaa ggttaaaaaa cactgagaat aaatcataac
    41341 cgcgaaaaca tggaatgatg ttaaaaatag caagttgacc cagaatatgt gccatgacta
    41401 aaaaaaaaat tggcacaaaa atataaagga atattaattt tggtttcaat tttatgatta
    41461 tttgcgacag attctggtcg ttcgtatttg acatgcttca tttcatccct tcagaccata
    41521 tttcttgagt ttgtatcgca gcatccgttc cgatattccc aacagatcgg cggcccgggt
    41581 ctgattgttc tgggtttttt ccatagcctc aataattaat tttttttcca attcatccag
    41641 agagtctttt aacgcgccga aaggtttctt ttctgaatct tcaagcagga aatcagattt
    41701 gaacggcaga tcattggtgg taatgtattg ttcgcgtgcg atgacgacag ccctctccag
    41761 aatgttttct aattcccgaa cattgcccgg gtaatcatat tttaaaagta aatccctggc
    41821 ttcagccgta aatcctttta tgtttttgcc gttttcttta gcaaacttat cgataaaata
    41881 atcaatcaag acaggtaaat cttccttgcg ctctctgagc ggcggcagcg acatcgtaac
    41941 aacttttaaa cggtaaaata aatcatcccg gaaagttccc tctgccactt gtatttccag
    42001 gtcgcgattg gtggcgctga taaaccgcac gtcaacctgg agattgacat tgctgcccag
    42061 ccgctgaaat tcacgttctt gaagaaaccg caaaagcttg acctggatgg tcgggctcag
    42121 ctcccctatt tcatctaaaa agatcgtgcc tccgtcggcc tcttcaaacc ggccgatgcg
    42181 gcgcgaggac gctccggtaa aagcgccttt ttcatgaccg aacagctcgc tttccagcag
    42241 attttcatgg agtgcaacgc aattcacaat aataatcggt ttatcggcac ggggactcaa
    42301 ttgatgaatc agccgcgcca acaattcctt acccgtgccg ctttcgccct gaatcaaaac
    42361 ggaagctttg ctgggcgcta cgcggccggc cagattgata agttccgcca tttgccggct
    42421 ctgataaata attttatctg cagtaatgcc gtgtttgctc aagtcatcct tcagcagcct
    42481 gttctcctgg atgagttgtc gtcgctccgc tattctgttg accagaataa tcagttcatc
    42541 cagttccacg atcggtttgg tgatatagtc aatggcgccg gccttcaggg catccactgc
    42601 cgtctcgaca gtgccgtatg ccgtgatgat caccacatcg atttccggat tgattcgttt
    42661 gacttctttg agcacttcca gaccgttcat ccccggcatt ttgtaatcca gaagaatcag
    42721 atcgaaatgg ccgcccaaaa ccattttgat tgctttttct ccgttatccg cttctgtgac
    42781 cgtatggcct tcgcttcgga gaaaatcgcg taacagttcc cgctgggatt tttcgtcgtc
    42841 aacaatcaga atgctcagct tttgcatgaa atggtcatcc ttgtttgatt atgaaacttc
    42901 attcaatctt tcccggggaa gaatcacttc aaatgttgtg cctttattct tccggctggt
    42961 tacccggata tcaccgccgt gtccacggat gatttcaaag gccagtggaa ttcccagccc
    43021 cacccctttt tctttggtgg tgtattcggg attaaagatt ttttcgagtt cttcttcggt
    43081 catgccgcaa ccggaatcgg aaatttttac aacgatgtaa tttttgcctt ccttgtccac
    43141 cgaaatcaca attctgcctt tttccgccgt tatggattcc atggcgtttt taatcagatt
    43201 gataaatgcc tgctgaagtt tgttcacgtc catggagatt ggaacggact cctgtttcca
    43261 ggttgtttca agggtaattc cgcgcaacag ggcttcttct ttcatcaggc taacgatttt
    43321 ttgcagcagt tctgtgatgg aaaatttaca gaaatccagg cggcggcttt tggaaaaatt
    43381 aagaaattct tcaatgattc cattgaggcg tttaatctca tccctgatga tgcccgataa
    43441 attctgaaaa tcgtcaacct tttgagggtc ggaggggacg aaatctcttt tcaatctctg
    43501 ggtggccatg ctgatggcgt tcaatggatt gcgtatttca tgggctactc cggcagccag
    43561 ttgacccaga gaagaaagcc gttcagcttt ttccaattgg cgttccattt ccacaatgcc
    43621 ggccaggtgg cggttctggt catggtaaag cagccacatg gacaacaagg cgatcaggac
    43681 gataaatatc gagaaaacaa aaatatgttg tctattttcg acaagactct tatccatgga
    43741 gtttcggtca aggccgatgc gcacaatacc cacaattttt ttatccagat acaacggcgc
    43801 cgccaggtcg agaattttat tgtcggcata atccactttc cggctgacaa tttttatttt
    43861 tccggacaaa atttccttta gattgaagat tctgtgattt aaagcggggg ccggctggcc
    43921 gacactgctg agcagcttgt cccggtcatt aaagatttgc atataaacga tgccgtggcc
    43981 ttcgccgatc ttgtccattc ccctttcacc ggcaattttt aaaatccagt aaacggatcc
    44041 ggatttgtcc aggctaatga tcaccgctcc ggtattgtcc ttacggctta atgcaataaa
    44101 tcctactcct tgtttctctc ctattttcga tatcagttcc agcgtggtca atttacgacc
    44161 tttgtgcgta agctcgtttt cctcaaggag gtctctctgc aaagggccgc tctgaaagac
    44221 agcgtttcct tgagcattca acatccccac atacgaaaaa ttattgtccg atgcgaattg
    44281 acgcagggac tcatcggtca tgtccccttt tcgccattgt tcatccaaat ctttggccag
    44341 ttcggtaata gcctggataa cccattgttt agaataggat gcttcgtcac gaccggacgt
    44401 tttttcatgc ggagcgttct tatccggtac gccgataatg ctttttaaat tctcttccgt
    44461 taagcgctga agaacgccga tagtgctcaa agcctgattt tccataaagg ctatcaggtt
    44521 gctttcgctg cgtcgaagct cgagaaatcc tgtaatcaga attaatgcga caaaaattgc
    44581 actgacgata gccacggcca gaggttgaag gaaggggtca ttgtaattat gagctttgcg
    44641 atgaacgacg atgtcttttt ttctgaaaag acgcattgtt atccttaata attgattttg
    44701 ccggtcattt taagctattt gaaagaaaag tcaacgatta atcctttttt atccgacaat
    44761 tttgtcgaat gccggacaaa cagcgtcaaa aaaacttaat taattgtaat tattatgatt
    44821 tttaaaaatt aacaaaaatg attcgacatt ttttgccgct aaacaagtcg cggatattcg
    44881 ctgaagaagg cattaatata catattttgt caataatatt aatattatgt aaaatattta
    44941 gaattctggt acggcaaatg ctttaaatac gggcgggtaa attgtcgact gaaaaataac
    45001 gggtatgatt agccgacaac aaaataacct ggaaggtata aaaaccttca ctcttcatgt
    45061 ttaagggagg ctccgaccaa gcctccctta ctttttaatg acactcgctg agaacgagct
    45121 tagcgaagtt cgaagcgcaa gtggaattat ccccgaagcg tagcgtaacg ggatttgaca
    45181 ctcgctgata gtgaggactc gttgagaacg aatgaaatga tgttcgaagc gttagccgaa
    45241 ctataccagg cacgtagtgc cgtggtagaa caagcgaagt gcagtttgag acataagtcg
    45301 aatcaatgaa actccaattt caaaagcaga acgcattgaa attggtaagt tgaattgtcc
    45361 cttgtgcgga gcacatgggg atatcccaca tgcgaaactt agtgcgattc taggcgcttt
    45421 attctttttc cccttctttt ttcttatttc gccagtaatt gagtcgttct ttaattgttt
    45481 tttcaaagcc cacgcctttg ggctgataaa ataactctcc cttgatttct tccggcaaat
    45541 aatcctgcgg aacataactc tcttcgtagt catgagcgta tttgtaatct ttgccgtagt
    45601 tcaggtcttt cattaattta gtcggagcgt tccggatgtg taatggcacg ggcaaatacc
    45661 ctttggtatt gatggtattt ttaactttgc cgtaaccgac gtaaagagca ttgcttttgg
    45721 gcgctgtggc caggtaaaca gccgcctgag ccagggccag ttcgccttcc ggcaggccga
    45781 tgaagtgaaa tgcctgctgg gcggcaacgg cgacaaccag cgcctgagga tcggcattac
    45841 cgatgtcttc agaagcaaag cggatcatcc gccgcgcgat ataaagggga tcttctccgg
    45901 cggccagcat ccgccccagc cagtaaagag ctgcatccgg atcgctgccc cgcaagctct
    45961 tatgcagggc ggaaatcaaa ttgtaatgtt cttcgccgtc tttgtcgtat aaaagcgatt
    46021 ttttcagcag ggcatcctga gcgaatttca gcgatattgc cctctgatcg tccggcaggt
    46081 tttgtaccat ggataccagc acttcaagat tattcaacgc acttcgggca tcgccgtcag
    46141 ccagactgac cagatagcga agaacattgg catcaatttt cacatcgata ttgccgaggc
    46201 ctttttgctt gtttttaagg gcggcctcca gaatagcaag gagattttct tctgaaaaag
    46261 gttttaaaac cagcacccgt gtccgcgaga gtagaggagc gatgacttca aaagagggat
    46321 tttccgtggt tgcgccgatt aatgtgatca agccgctttc cacatgcggc agaaaagcat
    46381 cctgttgcgc tttattgaaa cggtgaattt catcgacaaa taagatggtc ttgatgcttt
    46441 ttctctccag caattctctg gcttcgtcaa tgaccgcgcg gatttccttg acgccggaaa
    46501 ggacggcgga gaaactgaca aattttgatt ttgtttcatt ggccagaatt cgagccaatg
    46561 tggttttgcc tgagccgggc ggcccccaga aaatcattga aaacagcttg tcggattcga
    46621 tcgcccgttt gagcagagat ccttcaccca gtagatgctt ctggccgatg tattcgtaaa
    46681 tattctgtgg tctcatccgc tcagccagcg gcgctgcgtc tttgacgttg ccggaattcc
    46741 cctgatcgaa taaatccaga gttttcattt ttttgctttt gccgcctcaa tcagttctat
    46801 caagcttgtt attttgtccg gagctttggt attgataaac ttgagccaac gtttttcaga
    46861 tagatgcccc ttcaaaaaag attctatatc gaaaacagga gaccagcagc ggatgattcg
    46921 aacacaggga agattgcctc cttcacgcag acagtaagca aaagtcattt cgtctcctaa
    46981 cttcgggcag cggatcattt tgttgtcgtg ttgtgttttc aaggggttca gctttactga
    47041 aaaaagaaag gccgtcaaga tataaatttc tcaacggcct tatctgttgt tactcagggt
    47101 ttactagggt ttggggaaat ttttaatggc ttttaatgct cgctcgatct cgtgaccggg
    47161 cagttcataa gctttcaatt tacctgcaaa ataagcatcg tacgcggcaa gatcgatgat
    47221 accgtggccg ctccagttaa agagaatggt tttttccttt ccttcctttt tggcgcgcat
    47281 cgcctcgtca acaaccgctg ccagtacgtg attggtttcc ggtgccggga tgaaaccttc
    47341 ggaacgggcc catttgacgc cggcgtcaaa tgtcttcaac tgatcgtagg ctctgggttc
    47401 gataagtttt tccttgacca gatgactgat gatcggcgcc atgccgtgat agcgcagtcc
    47461 gcccgcgtga atcggcgccg gcacatagtc atggccgagt gtgtacatgg gcagaagcgg
    47521 tgttttctga gcgagatcgc cgaagtcata tgcaaagggg cccttggtca tggtcggaca
    47581 ggaggacggt tccgcgccca ctattttcac atctttaccg tgaattttat cacgcacaaa
    47641 cggggcggcg atgccggcaa aattacttcc gccgccacag catcccataa ccacgtccgg
    47701 gtagatgccg agtttctgca tctgtttttc cgcttccagg ccgatgatgg actgataaat
    47761 caacacgtga ttcaacacgc tgcccagcgc gtaatgggtt ttgattttgc tggtgaccgc
    47821 atcttcgatg gcttcgctga tggcgatgcc gaggctaccg ggagaatcgg gatacttggc
    47881 cagaaaatct ctgcctgatt gcgtcagcgt gctgggactg ggaatgcatt ccgcgcccca
    47941 tgtgttcatc atcaggcgac ggtagggttt ctgttcgtag ctgattttaa ccatgtatac
    48001 acggcactcc agaccgaaca tttgcgtagc catggccagg gcactgcccc actggccggc
    48061 gccggtttcc gtcgtgattc tcttggttcc cgcgaccttg ttataataag ccatcggtac
    48121 ggcggcgttc ggcttgtgtg aacccgcggg actcacgcct tcgtttttat aataaatttt
    48181 gacggggcaa ttgagcagtt tttcgaaatt ataggcccgg taaagaggag aaggacgcca
    48241 caatagatat ttttgcagga cttcttccgg aattttgatc cagcgcttgt tggaggcctc
    48301 atgttcaata atgttcattg ggaaaattgc cgccagatcc tggggggtta ccggttgacc
    48361 ggttccggga tgcagtggcg gtttcatcgg cgtaggcaga tccgccatga tgttatacca
    48421 ctttttgggt atctcacggt cctgcaatac tacttttacc tgttccatat tctttctcct
    48481 tcaaataaag tattttgatc ctgaaattaa gaaagccacg ggattgtatt cccatggctt
    48541 taactattac cggagagtca tgggaagaag cttccctgcc catgctcata ttttcccgag
    48601 attatgggca gacatttgaa atctgccacc accagttgtt tttggaaacg gttttgaaca
    48661 tagtcggctc acgtgtttct taaagttgga aattccgtag cacagcgaat aaatggatgt
    48721 caagttgaaa ttttgacaac ttacacgtgg tttctcaact gaagttctgc cggatgcggg
    48781 ttcaggtaca cctgatcttt gagataaggt tcgtcgtact tgcgcacata gtggctgatc
    48841 agcgtcaccg gcacaatgag gggaatatat ccgtcgcggt aagcatgaat caattccagc
    48901 agttctttct tttcatcgcc ggacaattgg tctttaaaat atcccatcat gtgctgcaga
    48961 acattgacgt gtttggccgg tgttgccttc accttaagcg attccagcag caacgcctga
    49021 tattcttttt gaatatccgg tccggcaaag tttttttgcg ccgccaccag tttacccatc
    49081 atttgataat gtttggtgct gtgcgcgagg atcagtaatt tatgtttggt gtgaaaatcg
    49141 atcaggctct ttttgaaatt tttcgcgctc aacgtttccc gccatctttt tagcgcgaat
    49201 attctttcaa taaagttttc gcgcaggccg ggatcgtgta gccggccttc atcttccacg
    49261 ggcaacagcg gaaaatgttt catgaacatt gcggcaaaca gtcccacgcc gtttttcacc
    49321 ggcatggttt tttcgttgta gatttttacc cgctccatgc cgctgctggg tgaatcgctt
    49381 ttaaaaatga aaccgcagag atcttctttt tccagttcgg tgacgcgttt tttcgcccag
    49441 gtttccatgc gttcggtcag atcctgtctg gtgctggtaa ccattaaccg cggcgcgtcc
    49501 ggatgccctt ccaggcgcat ggaaggtctg ggaatactca ggccgcattc gacttccgga
    49561 caaaccggca catagtccac gtattttccc agcgtatcgg ttaaaaacct atccaattta
    49621 tgtccgccgt catagcggac attgttgccc agtaaacagg agctgatacc cagtctgatt
    49681 ttttccatgt gaaatccttt gtagtgttat aatagtgaag cgggatacgc tttaaactga
    49741 atacttaaat tttaagtttt tcatctgctc tgagccgctg cgctaataat gtcaacatca
    49801 agatgtgatg agcgctacct ttacaatgaa ctgattcatc gtgcaagagc cctgttgcat
    49861 gatgcaagga caacgcatga atcaatcgag tcgaagaatc ccttttttta acttgcgatg
    49921 aatggtggac ggattgatgc ccagtaattt tgcggcgcca acaatgctgc ccgtcatttg
    49981 atacgcctgt ctgatccatt tctcctcaaa ctgatccatc gccttgggca gggaaggcgg
    50041 cagcacgggc atagcgggtt caggcgccgg aagagaccgg tcgctgctgg cttgacgggg
    50101 gttgatattc aggatcagtt ccagatcttc ctcagtcaac tcctgagatg catgcatgat
    50161 agccatccgc tcaataacat ttttcaactc ccgcacgttg cccggccatt cataagtata
    50221 cagtcgattc atcagcttcg ccggaagttt gatgtccgtt ttatattttg tgttgaatat
    50281 ctgaatgaaa tggaggatca gaggcgggat atcctcctgc ctgtcacgca gcggcgggat
    50341 gaaaaccggg atcacagcga tccggtaaaa gagatcctgc cggaactgtt tgctgttcat
    50401 gagttggtcc atggtgagat tggtggcgca aaggatgcga acatccaccg cttgcgtctt
    50461 gttgctgccg atcttctgga aatttccctc ctggaggaat cgcagtaatt tactttgcat
    50521 tttcaggggc aattcgccga tttcatccag caagagcgtt ccttcatgtg cctcttccag
    50581 gaggcccttc ttgccgctcc tttttgcacc ggtaaatgaa ccttcttcat atccgaataa
    50641 ttccgcttcc agaagttcat cgggaatgga ggcgcagttg atggcgacga acggtttggc
    50701 gctgcgggcg ctgagctgat ggattaaagc agccatgatt tctttgccga cgcctgtttc
    50761 tccccagatt aaaacggggg aagaagtggc ggccgctttc catcccaggc ggatgacctg
    50821 ctgcatgacg gaactggagt agataatctc cacctggcct gcgtccccgg ccatctttaa
    50881 gtttctgccc tcatactggg atatgatcgt ttcagaaatt ttgagctttt tttccagagt
    50941 cacgagtttt gtcatgtccc tgccggttcc gacggtcata acgagttcac cccgttcatc
    51001 gaagaccggg atataagtcc acgtagccgt ttgtccggtt tcgtaggtca gattcatgtc
    51061 aatttttttc ttgacttcca gggacttgag ggccgcggat tctctcagta ttccgctggc
    51121 cacgatgtcc ttcagattca tcccgacgag cgaccggccc atcaaatcgg tcagtctgct
    51181 gaaggtctcg ttgtatttca ggatgttggc gtcacgatca acgattaaaa tagcctcgtc
    51241 aatacaattt agcaggacat cgatatcttt catcagatat ttttgaatca ggtccacttt
    51301 tgctccttcg acacctttcc tataagcagt tctcttgcaa gttgcaacat attttaacgt
    51361 aaaaaagggg cagcatatta ataatccaat tatttctttg acttacattt tggcattaaa
    51421 tctgcaatat aaagcgatag aaaattttaa tatttacacc gaaaggagaa aaattatgtc
    51481 caatcaaaaa ccctggctga agttttacaa aaatgtgccg gaaacgatcg attacccccg
    51541 cgtgacgatg tacgaagccc tgatgcagac ggtcgaaaga tatccggacc gcatcgctta
    51601 tgattttttt gattacacat caacgtatcg ccagtttgcg caggatatcg acatctgcgc
    51661 caacgcgctg gcctcgctcg gcctgaagaa gggcgaccgg atgacgattt ccatgccgac
    51721 ctctccgcag ggtatcattt gcttttacgc cgccaataag ctcggcgtgg tggccagtat
    51781 gatccacccg ctctccacgc ccgccgaaat cgcctattat ctgaatgtca gcaaaagccg
    51841 ggttgccttg acgctggatt tgtttcataa cagtttcaaa aatcttccgg aaaagacgcc
    51901 gctggagctg ctgatcctca cggaaatttt tgaccagctt acgcaggcgc agcccatggc
    51961 ggaaaaaccc ttgccggaag agggaccggc tgtccggtgg tggaaggaac tcatgactgc
    52021 cgcgcacccg caggcgccga aagcggacat gcacacggat gaaatggccg tgatccttta
    52081 cagcggcggg acgactggct ttcccaaagg catcatgctc tccaacttca attttatcag
    52141 tgagggactg atggtggcca actggggagg cggcgaactg atggcggagg attcttcggt
    52201 tctggccatc ctgcccattt tccacggttt tggtttgggt gtttgctgca acgccgcttt
    52261 tatgggcgga agcaagagca ttcttgttcc ccagtttaca ccggagaacg tagccgatct
    52321 cattcgaaaa aaacgcccca gcattattgt cggggtgccg acactttacg atgccctctc
    52381 caagaatgaa gtattccaga ccacggacct gagctgtctg aagaatacat cctcgggcgc
    52441 cgattccctg ccgcggaccg tcaaggacag gtttgaagaa gtcgtgaaga gacagaatgg
    52501 aaacgtccag ctggtcgaag gctacggtct gacggaagcc gtcaccgcga ttatgtccac
    52561 gccgattgac ggataccggg aaggcagcgt gggcattcca ttccctgata tgctggcgaa
    52621 gatagtcggg atcggcacca tcgacgaggt tcctgtcggc caggaaggag agctttgctt
    52681 gcacggaccg gccgtcatgc tgggctacct ggagcagccg gaagagaccg ccagtgttct
    52741 caaaacacat gctgacggca aggtctggct tcacacgggg gacattttca ccatggatgc
    52801 agacggttat ttctattata aactgcgtct caaacgcatg atcaagtctt cgggcatgaa
    52861 tgtctatccg gttcaggtgg aagcttgtct ggatcaacat ccggatgtgg cggcagtctg
    52921 cgtgatcggc gtgccggatg aggctcaggt ggagaaggtc aaagccttcg ttgtcctgaa
    52981 ggacgctacc aaggcatctg ccgaaatgga aaaagaactg attaactttt cccgcaagca
    53041 tctgattaag tggtcctgcc cccgggaagt ggagtttacg gacaagctgc cgctgacgaa
    53101 agtcggcaag attgcctaca ccgaactggc aaagcaggaa attgaaaaac tccgcaaagc
    53161 gggaaaatat acaggaggaa aataatgaac catctggagg aatatcgccg ggcggtctcg
    53221 gacccttccg attatgtcca acaactgaaa aaggcatcgg ggaagaaaat catcggatac
    53281 acgtgctctt acacacctga ggaagttatc atggccgcgg gcgctcatcc cctaaggctt
    53341 ttcggcacaa aacagcatat cagcctggcc gattcccact tgcagtctta ctgttgctcc
    53401 gtggtccgcg gaattctgga ggaaggtctg tcacgacggg ttgactacct ggacggcatg
    53461 gtttttccgc atacctgcga ctccatgcaa cggctgtccg acatctggcg gatgaacatc
    53521 cccttcggtt ttcatctgga tatcaccctg cccgttaaac tcgatacggc aagcgccaaa
    53581 gattatatga tcgacgtctt aaaaaaattc cgggatgact tgggcgggaa actgaacgtc
    53641 gaaatttccg atgatgcggt gcaaaaagcg atcgcgacca ccaacgctat ccgtcaatcg
    53701 gttcgggcga tctatgacat ccgcaacctt catccccggc tcatgccggg agaggatcta
    53761 tacaacattg tccgcgcgtc catgatcatg gatcgggacc ggctggccgt gatgctggcc
    53821 gaaacagccg ccgaattgaa agaacgcgcg accagtgcca agcccgcctc cgtaaagcgc
    53881 atcctgatgg cgggcggcat gtgcaatcag cccgacgtct atgccatgat cgaagaatcg
    53941 ggaggcgaga ttgtctggga tgatttctgc acgggggcac gctattttac cggtttgact
    54001 gacgccggaa gcgatccgct ttcgagcatt ggcgaacgac ttctgaatcg ggtggtctgt
    54061 ccggcaaagc atgccgacct tgatggtcgc gcccgacata ttatccagct ggcccaggaa
    54121 aaaaacgcgc aaggggtcat tttcctgttg cccaagttct gcgatcccca tgccttcgac
    54181 tacccctacc tcaaagagcg tctcgaaaaa gccggcattc cgctgatgaa cgtggaagtc
    54241 gatgattccc tgcccgccga agggcagttg aagactcgct ttgaatcttt tttagagatg
    54301 ctgtgaaagg agataacatg tcagatacac aagataccga aaagaaaacc gtcagctaca
    54361 caaaacagat gcgggatatt atgaccgcct attatattga cgcaaaaacg gcccgggaaa
    54421 ataataaaaa agtggcctgg atcaccagcg gcggtccggt cgagccgttg attgccatgg
    54481 atatcattcc cgtttacccg gaaaattacg gagccatgat cggtgccagc aagatgggcg
    54541 tcgatttatg caagaaggcg gaggacatgg gctattcgag cgatctttgt tcttacgccc
    54601 gcggcgacat ttcctgtgcg accgtcaaag gcggccctat tggcggcctg ccggaaccgg
    54661 acatgttggt atgctgcaat aacatctgcg gcaccgttct caaatggtat gaagtgcagg
    54721 cccggtattt caaggtgccc ctgttcattt ttgatacccc cattgtccac acggaattca
    54781 cgcgggagat gaaaacctac gtgcagcggc agatggaaga atatattgtt ttcctggaaa
    54841 atgtttccgg ccgcaagatg gatcgggacc gcatggtgga ggtcggcaag ctctccgtcg
    54901 aggggcagca gctctggcag gaggttttag atacgactat gaatagaccc tctcccatga
    54961 ccgccttcga tgccttcttt tatctcgccc tgatcgtcac gctccgggga acgaagcagg
    55021 ctgttgactt ctaccgggga ctccgggacg aaatgcgcat acgggccagc gaaggaaaag
    55081 gggccattcc ccgcgagcga taccgcctgc tgtgggacaa cctgccgatt tggtaccggc
    55141 tcaaatggct gtctcaggaa tttgccaagc acgacgcctg cctggtcgct gacacctaca
    55201 cctcggcctg gtgcggttct atgaaataca ttgaccggaa tgattttatt ggctccgcgg
    55261 cggaaaccta cacacgcatt tatctgaata tcggcgtgga caagatggcc gagatagtcc
    55321 tttcgatgat tgacaaatac caggcggatg gaatcgtcat gcactccaac cgtagctgca
    55381 aaccttattc cttcggtcag tatgacatcc agaaaatcgt tgagcgcgaa cggggcattc
    55441 cctcattgat aattgaagcc gatatgtgcg atgagcgaag cttttccgag gcgcagattc
    55501 aaacgcggat cgacgccttt atggaaatga tccgggcgaa aaaaggggca tcctgaaatg
    55561 tgcactgccg ggattgcaag accttgccca ttagggtaaa gataatatgc cgtacccagt
    55621 gacacacggg attctctcgg caacaccaaa aaaaaattac gggggtatat gtcaatgaaa
    55681 gttcgaaaga ttatcttagc tgttatctgc acactgatgc ttgccgcaac agctgtcatg
    55741 gcaagtggcc catcgttgca gggagaagaa ctctacaagt acctttcaaa aaaaaccatc
    55801 agcgaaggag atgccgcgtt cgtggaaggg ctgaacacaa gctcgctcaa gctcgactgg
    55861 tctgcggcgc aaaagaagga tctgaccggc aacctccgca tcttgaaagt taagaacgcg
    55921 aaggtcggga ccgaggcgct ctattttata cgcggcagcg aaggagatat gtacgtcgta
    55981 tcactgccgg ccgaaggcgg gcgccccgcg gccgagtatg agaaaatctc ggaaaacagg
    56041 atgatgttcg cgctcgaaac cgttaccgga gaacacgaag gcacgaccta tgccttcgcc
    56101 cggatcagcg gggagccgaa ggctattctc ttcgaccgca tattcaagat agccatcgtc
    56161 ctcatgctct tctttgttat ggtcggtatg ggactgaccc tgaagctcag tgacttcgcc
    56221 ctcgtcttcc gcaagccgct gggcatcgtt atcggaatag tcctccagta cggcctgatg
    56281 cccctggtcg cgttcggcct gagctatatg ttcggattct accaggccta tccattcata
    56341 ttcatggggc ttatcctggc gacggcgagc cccgcgggag tcacctcgaa ccttctgaca
    56401 caattcgcaa agggcgatct ggcgctttcc atttccctca cctcgatctc gacgatcctg
    56461 gccatgttct gcacgccgct cctcctctcc ctttacggga acaccagttc gggagcatcg
    56521 gttccggccc ttctcatcgc acagaccatc atcgtgctgg tcatcgttcc actggccgta
    56581 ggcatgacgg tgcgggccaa attcgagaag gccgcgctca aggcggctcc gatcttctcc
    56641 atcctaggaa ttatcacggt cctgttcatc atggtgacgg gcgtggtggg aaatctcaac
    56701 gcgctgaaag acaccgctcg ctacagcctc tccttctacc tcgctcccct gattttagct
    56761 ctcaccggca tggtgatcgc gatagtcgtg gcgaagattt tccgtgtgtc cccgaagcag
    56821 gtccggtctc tcgctttcga gactggcgtc cgcaactccg ccctttccat gacgctcgcg
    56881 atcctcattc aggaccagat cggcgatttc tatagctcgc tctttatcgt caacggggtc
    56941 tatggtctgg aaatgtacat cgcgggtata cttttcgtga tgttcttcag gaaattttcg
    57001 aaggaaacgg aagggtagtc atctcactct cggtttcttt ttcgagagag ggcaagggaa
    57061 tcccccgaaa gttgtgccag cgttaattag agtgtggaac acaaaaacac tttaaggagg
    57121 cacagatgaa gaagcgattt acagaagagc agatcatcgg ggttttgcag gaagccgaac
    57181 gaggggagaa agtcaaggac gtatgccgca aatgcagctt agccggacgt ctgtttttac
    57241 ttgaccagat gagtttctta tactactttt aacacaagta aattttcatt acgtcaagca
    57301 ctgaaggcca gtaataaaat aattcaacag agaacctttg aagaaaaaaa ttacaagaat
    57361 cggaggtaaa aatgaaggac gtaacttttc tgacccataa gggcgtaaga attttgtatg
    57421 aagatcttga aaatgccaaa caggaggacg tattaccctg gatcgaaaga gctaaaggag
    57481 ttattcgcag tcagcctgaa aaatctgttc ttgctctgct caatgtcaaa aatgcaaaat
    57541 ttgacacaga agtaacagcc gccctgaagg aattcgcgaa gggaaacgaa ccttatgtca
    57601 aattcgctgc tgtttacggt atagaaggtt tgaaggaaat aatattcaaa agcgtcctga
    57661 ccttcaccgg caggaagaac cttgttcttt gtaagaccct tgaagaagca aaagatattc
    57721 tggcgagcaa agtttgaaac aagcgaaatg atttttgtcc ctgccaccag aaagtggcag
    57781 ggtaattccc tataagattt tccgatcact gcgtatcaga aaatcaaggt tcattaaaac
    57841 ctaagttgaa tgggttcttg tgcaaagcac atggggatat tccgagaacc aagtaccctg
    57901 aataacctaa atgtaaacag gggagaattc ataacgacga gatatttctt aagagttggc
    57961 ctagcaataa agaaaacttt ttggagtgaa gattatcaaa gcgcaaaaac ttgcctattt
    58021 tgacacatcg catgagatgc cgctggaaat tatcatggcg gccggattta ccccgtataa
    58081 aattctggga gatgtccacc aatccaatga cccagcggat aaacatttat ccaacttttt
    58141 ctgtggcgcc gcaaagagct ggctcacgga agctctggcc cgctccaaag aatgggccgg
    58201 cattattttc gcgcaggggt gcaacaatac caaccggcat tatgacattt ggaaggttca
    58261 tgtccaaacg cctttcctgc actggttcgg cgctcctctg ctggacagtg acgccgcgaa
    58321 aaaatattac cgggttgaac tgcaaagact gattagccat ctggaaaatc aatttaatat
    58381 aaaaattacg cctgaaaaaa taagagaggc gattatcaaa tccaacgaaa tcaagaaaaa
    58441 gctgcaacgt ctctctgcgc tcagagcaca gaaggacatt cccaaccgcg attatcttgc
    58501 cgcgatgata aaatgtctta cgaatcccgt ggatgaagtt tcggcttggc ttgactcaga
    58561 gatagcggcc tggaattcaa agccctcttt tccgtccggc aaaaaacaat ttttattaac
    58621 cggcagcgac atcacgtatg tggaatggat ggacacgctg gaagattgcg gaatacggat
    58681 tgttcgtgac gacctttccg taggagaaag atttttcgcg acactgattc ccgacaagag
    58741 cgatccgctg gatgccctta ttgaatatta catgaacatc cccaagcccg cgacaaggcc
    58801 gaccattcag aaacggttgg actatcttta taaagctctc ggtgagacaa aggttgacgg
    58861 tgttctttcg caaaatgtga aattctgcga gccttttgcc tatgattccg tgcccgtcaa
    58921 taatgccctg aaagacaagg gctacaaggt gatccacctg gaacgagact tttctcctgc
    58981 ggccgaccag caactcgcca accggcttat ggcctttgtg gaaatgatgg aggtagggaa
    59041 caatggctaa aacaaacaat tctgctgaga atgacagaaa gataaaactc aaaaccctgc
    59101 ctcacgcgaa agacctgaag tggccgatgc tgtggtggtt taccatgggg tcaatatacc
    59161 atgctttgcc ttggaaaaag acggcctgga catgtgccgc ctttcccatt gagctcatgt
    59221 ggatttacga tatttttccc gatcaccctg aaaatatggc aaccgtggcg gccgcccgca
    59281 agcagtccca gcgtctgatt gagcatgccg agggaatggg ttattcacgt gacctgtgtt
    59341 cctactgcaa gacaagcatt gccgcctacg ataccaaaat caaaaagacg ctcggcggaa
    59401 tcagcaagcc cgatatcgtg gtgtgcacca atgccatctg cgatacccac tggaagtggt
    59461 ttcagattat ggccgataaa atgaacgtcc cgttttttat gtttgatatt cccaaatggg
    59521 tgagcggtac ggatgagaaa accctccagg gtaatattga ttacgtggta gagcagtttt
    59581 acgacctgat tgtatttatt gaaaagcaca ccggtaaaaa aatggatgag aaaagactgt
    59641 acaaagtttt ggataaatcg acggaactgt ccgacctgtg gcgggaaatt tttgaacata
    59701 gaaaggcggt gccgtcgccc tacagcggag cggagacatc ggcgtcattt tttcccctgg
    59761 tggttctgcc gggtgttcag gtcggcataa attttttcaa aaaaattctt gccgatatca
    59821 aaaacctcac cgcaaagggc gagggaacca tgcccaaaga caaagaaaaa taccgtgtga
    59881 tgtgggaagg cattcccttc tggtaccgga tgaggttcat gtatgacctc gcgcaatacg
    59941 gcgcggtcat taccttcgag ccttacacct actcgttcgc tcccgaaaag aaaaagtgta
    60001 acaccatgga agagacattg cgcgatgtcg ccaaactcat catggacacg ccctacgcct
    60061 acaacctcga aagacgtata gagcattttg aaaaatggat tgacgaatac aaaattgacg
    60121 gcgtcattct gcacgaaaac atgtcgtgca ggccatccag cgccggcatg gtcgatctgg
    60181 cggaggccat catgagggac aagggcattc ccgtgctgat cctccaatgt gacatgaacg
    60241 atccccgggc ctactccgaa gggcaaatta aaacgagggt ggaggggttt attgaactca
    60301 tggaggcgaa taaaggcaag taaatagaac cgttgtcttt tgctctgcag ttcagccaag
    60361 tctttttgac ttggctgtcg taaatatcgt gaactgataa aaggtcgagt ccaaaagact
    60421 cgaccaaact aggggaaaaa ttgtcactga ataaacactt ataatatcca accaaaggaa
    60481 taagaggtta gatattaaga aaggatataa tgattacagc aggaattgat atcggttcta
    60541 ttaccacgaa ggcggctatt tttgccgatg gtaagattac ggcgacaaaa gttattttta
    60601 ccggatacaa tgccgaagcg gcaggcctca aggtttatga agaagccctg aaagaggcgg
    60661 gcattgataa aaatgccgtg gcaaaaattg tttccaccgg ctacggccgc aacagcgtta
    60721 aattcgctga taagtcctac acggaaatca tgtgccacgc caccggagca cattttttga
    60781 atccgcatat ccgttccatc attgatatcg gcggccagga cagcaaagcc attcttctgg
    60841 acgacaaagg caaggtaaaa aactttgtca tgaatgataa atgcgccgcc ggaacaggcc
    60901 gttttctgga agttatggcc agagccatgg aagtcaatct cgatgaattc ggcgctatgt
    60961 ccatcaattc aaatcaaccc gccaaaatca gcagcctctg cacggtattt gcggaatcgg
    61021 aagtgatttc cctgattgca aaaggtgaac aacggaagga catcatcgcg ggaattcatg
    61081 aatccattgc ttccagaatc tcgtccatga tcggtcgtct gggcattaaa gagcccgtca
    61141 tgattacggg aggagtggcc aaaaacgttg gagtcgtgca cgctcttgaa aagaaactgg
    61201 ggctgaaaat cgaagtatcg ccttacgcgc aggttaacgg ggcgatcgga gccgccgttc
    61261 ttgcagcgtc attatagtca atctttttta cagggaaaat tgtcagcgcc atgtccacag
    61321 attacgactt catgaaaata gcccttgaag aagcccaatc agcgtaccgc cgtggtgaag
    61381 ttcccgtcgg agcggtgctt gtccgcgaag gaaatgttct ggcgcgtgcc cataattcgc
    61441 ccattgctct gaacgatccc tcggcgcacg cggaaatgca ggttttaaga caggccgcag
    61501 aaaaaatcgg taattaccgg ctggcgggaa cagagcttta cgtaacgatt gaaccttgca
    61561 tcatgtgcgc gggcgccatt atccacgccc gcgtggaacg ggtgattttc ggagcgcgcg
    61621 atccaaaatt cggagcggtt gtgtctcttt ataatatcct ggatgataaa cggttgaatc
    61681 atcaggtgaa agtaacggaa ggagtattaa aggaggagtg cggagaaatt atcagtagat
    61741 tttttcgcga aaagagggta acatccgcac ccgcttcggc gaagtgataa aatacggaga
    61801 ggtaccgaag tggtcgtaac gggatcgact cgaaatcgat ttgggggcca caagctccca
    61861 cgggggttcg aatccccctc tctccgccag aatacattgt tttttgaaaa ttggtttgtc
    61921 atttcgagaa acgatcatga cgagaaattg aatgggaaat gcaaataaag atttctctcc
    61981 ggcgtgcgcc gggtcgaaat gacaaagtgt tagaaacgga gagatgtccg agcggctgaa
    62041 ggagcacgac tggaaatcgt gtgtacgccc ccaaaggtgt accgcgggtt caaatcccgc
    62101 tctctccgcc attattttgt gaaactccaa ttccgaaagc gaaacgcaac ggaattggta
    62161 agttgaacaa tcccacataa atgtagggaa atgagaccca aatttcagaa acaaagtgca
    62221 ctgaaatttg atagtggaat tatcccttgt gcgaagcaca tagggatatc ccgcctaagc
    62281 aggaataata tagcattcgt gatttatcaa tcttctgtaa aaacaataac atcgtttgtg
    62341 aaatctagac gcaaaaaatt tgagttgatt cctgttcgtt tatctattaa aagtctcagc
    62401 aggttgttta tttaaaaata tggccgggtc ccgcgcaacg gaggtagtga gactccaatt
    62461 gcgcaagcga agcgcggtgg aattggtaag tcgaactatc tccaaagcgc tgagactaat
    62521 gcccggagtg acaaagagag attttgtttc ttttcattat atgaaaataa tatggccggg
    62581 tcccgcgcaa cggaggtagt gagactccaa ttgcgcaagc gaa
//
LOCUS       DAOSME010000006        56991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_725_length_56991_cov_23.562316, whole genome shotgun sequence.
ACCESSION   DAOSME010000006 DAOSME010000000
VERSION     DAOSME010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 56991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56991
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_725_length_56991_cov_23.562316"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1708)
                     /locus_tag="PLG98_01640"
     CDS             complement(311..1708)
                     /locus_tag="PLG98_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOE31980.1"
                     /translation="MKKFFLLPLAVFFLVAVGLNSFAEEKALTLSASEKLLFNFDFIP
                     VVLTASPDGKRIAYATRVEGKQFVIVDGKAQKRYDGIGSNVVFSPDSKRSAYRAQVGN
                     KQVVVIDGQEGKRYDTVGSNIVFSPDSQRVAYAAKTGKRCFIVVDGKEGKSYEGGIGS
                     NIIFSPDSKKIAYGVLSDKKQFVVVDQQEGTHYDSIGNNIVFSPDSRRMAYQAQSDKK
                     RFLVVDGREGKRYDDVRGDFVFSPDGKQVAYVVKSAGKWQVVVDEQEGKYYDGIGDDI
                     FFSPDSSRIAYQAQSDKKRFLVVNGQEGKGYERIGSTIVFSPDSKKVAYIAQIVNGKF
                     ASVIDGQEGKPYNNIGNGSLVFSPDSKRVAYAAQSGQKRFVVVDGKEGKHYDGVGSYT
                     LVFSPDSTKIVYVAQAENKQIAVVNGKAGEKEVNKYNSIVALGKKGFAFETSDRFRFL
                     AVKEKSVYSIEVKIN"
     gene            2354..3901
                     /locus_tag="PLG98_01645"
     CDS             2354..3901
                     /locus_tag="PLG98_01645"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOE31981.1"
                     /translation="MKKKKNRVYIFDTTLRDGEQSPGYSMNTEEKLMLARQLERLGVD
                     IIEAGFPIASEGDFDAVRQIAKEIKKSQVAGLARANKLDIDRAWQAIKGAAHPRIHTF
                     ISSSDIHLKYQLKKSREQVLKEAVAAVKLARSYTDNVEFSPMDATRSDKDYLVEMVSA
                     VIDAGACTVNIPDTVGYATPEEFGNLIAYLFAHVQNIGEAIIATHCHNDLGLAVANSL
                     AAIRQGARQVECTINGIGERAGNTAMEEVVMALQTRKDLFGLHTNIKTNQIYKTSRLL
                     TQITGIPVQPNKAIVGANAFAHESGIHQDGLIKEKITYEIMTPQSVGISDTHIVLGKH
                     SGRNAISHHLKKMGYQLNDEQINKVAARVKELADIKKDIYDEDLEVILSEEIYRGEDK
                     YSLLYLNVVSGNVAIPTATMEMQVGQDIVRKAGFGNGPVDATFAAIRNITKTNYPLMK
                     YVVNAITGGSDAQGEVTVQLKHSSYSVVGRGAHPDVIVASAKAYINALNRMEFLKNNI
                     KKVKIVE"
     gene            3953..5212
                     /gene="leuC"
                     /locus_tag="PLG98_01650"
     CDS             3953..5212
                     /gene="leuC"
                     /locus_tag="PLG98_01650"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOE31982.1"
                     /translation="MGNTITEKILMAHTGLRKISTGDLINARVDIALGNDVTAPIAIN
                     AFKQAGGEKVFNRHKVALVLDHFTPNKDINSAQQCKAVREFASQQKITHFYEGGLVGV
                     EHALLPEQGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGELWFKVPPS
                     IKFIIFGKLQKWVSGKDLILHIIGRIGVDGALYKAMEFTGETIARLTMADRFSMANMA
                     IEAGAKNGIFAPDEVTAEYIRKKAKRHYTFYSSDPDAVYADTLEINAATIEPQVAFPH
                     LPSNVKGVSKAGRVKIDQSLIGSCTNGRIEDLRIAAKILKNRKASSNVRLIIVPATPE
                     IYRQALKEGLIEIFLKANAVISPPSCGACLGGHMGILAEGEKAVATTNRNFVGRMGHT
                     KSEVYLANPAVAAASAVLGRIASPEEL"
     gene            5382..5885
                     /locus_tag="PLG98_01655"
     CDS             5382..5885
                     /locus_tag="PLG98_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOE31983.1"
                     /translation="MIFKGKVWKFGDNIDTDAIIPARYLTTSDPEVLAVHCMEDADPD
                     FMKKMKKGDMILGGENFGCGSSREHAPISIKAAGVPCVVAKSFARIFYRNAFNMGLPI
                     FESKDIVDAVKEGNEITVDSARGIITINGTDKFFKINPIPPFMEELIANGGLMQHIAG
                     KRKKNEK"
     gene            5875..6981
                     /gene="leuB"
                     /locus_tag="PLG98_01660"
     CDS             5875..6981
                     /gene="leuB"
                     /locus_tag="PLG98_01660"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOE31984.1"
                     /translation="MKNNNFKIAVFAGDGIGQEIINEALKVLKIISGKKNVDFQITEG
                     LVGGTAYDRYGTPLPEESLKLALKSDAVLLGAVGGPQWENLAYSVRPERALLGLRSSL
                     GIYANLRPIVVFEELLDASPLKKDIIRGIDIIIVRELTGDVYFGKPRGIKTSKNGKRK
                     GVNTMIYSEDEIRRIAVRAFEIARTRRKSLLSVDKANILETMELWRNVVTDVSRNYPD
                     VTLRHMYVDNCALQLIRDPSQFDVIVTTNLFGDILSDEAAMLTGSIGMLPSASLGEKT
                     ALYEPIHGSAPDIAGQNIANPLAAILSVALMLRHSLSMPAEAALIENAVTETLQEGLR
                     TRDIDQPGKKIVSTSAMGDAVAGNLNKRLAGIRP"
     gene            7015..7635
                     /locus_tag="PLG98_01665"
     CDS             7015..7635
                     /locus_tag="PLG98_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE31985.1"
                     /translation="MESVIFLEKISIEPPREKIYQRLGFKKRITAVSPDQQKQTDLFI
                     REAASLIVLKGMYLRLAVTKNDGKQIFLSNNVHFSSEHLSIFLRECREAVFMGATAGH
                     AVMDAIKAKTRQDDLAAAVVYDATASEMTDAALDWIMNYLNHLLMREGQTLLPRRFSA
                     GYGDFHLENQGIIYKQLHMQNAGVKINPNFILIPEKSVTAIGGICR"
     gene            7853..10471
                     /locus_tag="PLG98_01670"
     CDS             7853..10471
                     /locus_tag="PLG98_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE31986.1"
                     /translation="MKKNNAPPTGNPQPLQVLMLNDSDKDVSRIVSELQKGGYHPVYE
                     RIETAAAMRQALRDKPWDIILCDSNYLKFGAVAAMALLEEEKTDIPLIVISGASSKDK
                     TIECRFPGTRNYIVKENYSRLCPVIARELNETKIQAKHKQTESQKEAAQEELRRSEER
                     YRKITTCIPDLIWTMDLAGHFTYVNEVVERTHGWTVDEYLKLALQDMVTPQQLAKDTA
                     MMAEQLARSSEPDFDRNTTLTFESEELRKDGTTFLAEVTASFLWSDDGKPIGIIGITR
                     DITERKKAEEALRKSEELHIKLVNAIPDVVVRTDLAGNILFANENALRIGGYSKDEFL
                     GQNLLNFVAPEDRERVIRNLHLMLESRLGPREYHLMTRDGRKILFEINGDVLRKEDGT
                     PFAVVNVCRNITERKQIEKALREKDERLRGITENLPGVIFQFYATQNGKYGITYLSEP
                     IDEFAKILSKFDTDHLDTVFPEFLSRIHEDDRERFLSSITQAAQTASRWNFEGRVAIQ
                     SGKIIWFQGLSVPTRLPDRVIFDGILLNISERKLAEELSHQSEEKFYKIFMTTPDCIA
                     IARLHDGLIIDVNQGFEKIVGWKREQVIGTKSFEPPMNIWLDKTERKAMIADLKTGRD
                     ILDREFKFRRREGSERFGIYSARAITIDEQECVIFILQDITDRKLMDIELKRTLESLR
                     KSVGTTIQVMVSAIEMRDPYTAGHQLRVTDIARAIAAEMRLSQDKIDGLRMAGVIHDI
                     GKLSVPAEILAKPTRLTKLEYSLIQEHSRSGYEILKNVESSWPLAEIVYQHHERMDGS
                     GYPRNLKGNDILIEARILAVADVVESMASHRPYRASLGIEAALEEIEKNKGILYDEHV
                     ADVCLILFRHKGFILA"
     gene            10574..12973
                     /locus_tag="PLG98_01675"
     CDS             10574..12973
                     /locus_tag="PLG98_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOE31987.1"
                     /translation="MVEIINIRNFLKKKIMILDGATGTELQKLGLPAGVCPEIWCLDN
                     PDIIGAVHKSYQKAGAQIIYTCTFGANRYKLKQFGAKHDVYAVNLKLAQLARKACGNQ
                     TLVAGDIGPTGLFVEPFGTLAFEEAVDAFKEQARGLIDGGCDLIVIETMIDIQEARAA
                     LLAVKELADVFTIASMTYEKNGHTLGGTPPQSALITLQSLGADAVGCNCSAGPEQMID
                     FISAMKPYATVPLVAKPNAGMPLLKDGQTVFEMDPATFADFSVKLAAAGANMLGGCCG
                     TTPEHIRALRMSVTRKKPVLPLTKSVCALSSARRFLEIKDNDPLYIVGERINPTGKKS
                     LQQELLEGKLSIIRQMALDQENQGAVLLDINVGQPGIDEVQTIRNVISLLSTVSSLPL
                     VIDSSRIETIEAALRLYPGRMMINSISGEKEKLARLLPLAARYGAMFILLPLTGGEIP
                     RTAKKRRVVIDRIFRKAQAFGFTKKDFVVDGLTMAIASDADAARETLETIQWCKNIFQ
                     CKTILGLSNVSFGMPGRPWINAAFLAMAQHCGLTMAIANPASVEFMNVKNAGDALTAR
                     ERAAFHFINYFSQQTPAVAGLPSTAVSAGEKIVQAILEGNKDDITALLDEAMKSGKTA
                     SQLVDDYMIPSIVRVGDLYEKKIYFLPQLMAAAEAMKKALSHLDPYLQKATITTRGTV
                     LMATVRGDVHDIGKNIVALLLRNYGYRVIDLGKDVSEQAILDAAKKEKPDVIGLSALM
                     TTTMVNMKEVIALARAQGIRTDFLIGGAVVTDQYAKSIGAFYARDGVQAVKVVEKIIK
                     P"
     gene            12989..13711
                     /locus_tag="PLG98_01680"
     CDS             12989..13711
                     /locus_tag="PLG98_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOE31988.1"
                     /translation="MKHRHSAHQQIALIWALAFSVLIHFSAAAVLIGFELSGGAPAPS
                     DRNVLNMVWVSFDNKNADTGKAVQTGQQTIRQKKNTGRMTSVERPASPVGTVQLPLAS
                     RTAPAVESTFSEYENRPEINKSSVEARHSGGFEAVAQHPMSDIAIAYPLYRENTPPVY
                     PEMARLRGYEGIVLVFAEILPTGRVGDVKIRKSSGYAILDQSAIQAVKPWKFEPAKKA
                     GNPFTAWVELPIKFMLQHHPQS"
     gene            13737..14321
                     /gene="pyrE"
                     /locus_tag="PLG98_01685"
     CDS             13737..14321
                     /gene="pyrE"
                     /locus_tag="PLG98_01685"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOE31989.1"
                     /translation="MTIDQMKERLGEIILERSFKYSDNPPFTLASGRQSNYYFNCKPT
                     TLDPEGMNLIGTIIFDMLKDTDITAAGGLTLGADPIANALAVISYQKGKPIKSFIVRK
                     DAKDHGTKSAIEGSVRPGEKVAIIDDVITTGGSTITAIEQARKAGLSVVMVITLVDRE
                     EGGRENIMKHMDNIASILTRTEIMNLRERKAAQR"
     gene            14394..14792
                     /locus_tag="PLG98_01690"
     CDS             14394..14792
                     /locus_tag="PLG98_01690"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOE31990.1"
                     /translation="MKNTITVFFFIMCLLLAGCGAAVNLQGKVAGISSGRFFYQDGHL
                     VSDYKADIDAVWQACEQSVTDLKAKSIRKERKISTGVITSVIQDEKVTIKVEYVGRDM
                     TSVSVFVGAFGNTMASRLIHDRIIGNLAKN"
     gene            complement(14816..16051)
                     /locus_tag="PLG98_01695"
     CDS             complement(14816..16051)
                     /locus_tag="PLG98_01695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOE31991.1"
                     /translation="MSCNFKAENIFYATMITINYPEIILKKAREDAILRGHPWIFSGA
                     IGSVKGNPGPGDIVLARDAAGHPLALGFFNPSTDIAFRVLTRRCDENISPYFWQSRLH
                     DAYKLRQRLINEQTNAYRLINAEGDGFPGLIVDVYNTTLVFSIAVAGMEKQKNHLLNA
                     LIAQLKPTRIYEQSDSHSRKLEGLENRSGIAFGEDENSTVEIMENGLKFEVDVVSGQK
                     TGFFLDQRVNREKIGSLGRDAHVLNCFAYTAAFSVYCAEGGAKKVVSLDISKTACVAA
                     RKNLHLNWFSVENYPVIETDVFDYFRHTPENYNMIILDPPAFAKTKRDVPKAARGYKD
                     INLQAIKHLSKGGMLATFSCSNFIEEDLFYKIVLGAARDAGAELQLLSRLDAGPDHPV
                     AFGHPEGRYLKGLLLVKRN"
     gene            16112..17188
                     /locus_tag="PLG98_01700"
     CDS             16112..17188
                     /locus_tag="PLG98_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOE31992.1"
                     /translation="MYAVIMAGGRGVRFWPRSREKRPKHLLDITGEKTIIQETVNRIK
                     PLVPPTNILVVTGKKHARALMKQLPEIPPRNILIEPVGRNTAACIGLAALHIRKRSTD
                     DTMMVLPSDHAIADSGKYLSVMKAAAVVAAQEKGLVTIGITPSSVQTGFGYIEGGILC
                     KTIKNQDVFRVKSIREKPDFRTAAKFVRSGNFYWNSGMFVWQTSVILEEIKRWLPELY
                     AGLTLIDTSLGTAGETATVSRVYKKLGSVSIDYGVMEKAGNVFILPGNFGWSDVGSWD
                     ALWDISPKDKQNNVILSGSRTVLEDSKGSLIYNPRKLTALLGVRDLMIVETPDALLIC
                     KKGRSQDVKKIVERLEAGQLKKYL"
     gene            17615..18208
                     /locus_tag="PLG98_01705"
     CDS             17615..18208
                     /locus_tag="PLG98_01705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOE31993.1"
                     /translation="MSITAHASLILASASPRRQEMLRSAGVHVKIIPAHVNEKYLTGE
                     TPRQHVRRLSSLKAMKVAVENPDACVLGADTIVVIDGTVLGKPRNKTQAKTMLKKLSG
                     RQHTVFTGFTVVRLSFGIRSTGVVQSVVRFKTIHPDEMEWYIAGDEPYDKAGGYALQG
                     QGACFVRSIRGSYTNVIGLPLCEVLETLKKLHVIDFC"
     gene            18213..18905
                     /locus_tag="PLG98_01710"
     CDS             18213..18905
                     /locus_tag="PLG98_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOE31994.1"
                     /translation="MGTDIAANIAAVKQRIAAAAERGKRSPDSVKLLAVTKTVPVPVI
                     EEAIRAGITALGENYVQEAREKIAVIGRSIPWHMIGHLQTNKAKYAVILFDYVHSVDR
                     LDLAAEIDKRAGRAGRQINILIEINVSGEKTKNGIPAGKAVALLQDLSAMQHIAVKGL
                     MTMAPYSDDPETSRPYFRELKNLQQNIIRAGIAGIHMEELSMGMTDDFDVAVEEGATL
                     VRIGRAIFGERN"
     gene            complement(18937..20049)
                     /locus_tag="PLG98_01715"
     CDS             complement(18937..20049)
                     /locus_tag="PLG98_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOE31995.1"
                     /translation="MIVDDEDSILNAFKRILADENYDVHVANSGLEGLNKLRSATKPY
                     SIIISDQRMPEMSGVQFFSKAKEIFPDAIRILLTGYADSDAIIEAINKGGVHLYFTKP
                     WHEEEILLHIKQSLSKVEILAENKRLVELIREKNKELTELNKALEQKALEKTNDLLIQ
                     TEKLKESYQKSQIIMDGIVRTLSKIIETRDPYTAGHEDQVAKIACKIAREMKLPEEQI
                     SFIHIAATLHDIGKICVPSEILTKPSALNNLEKAIIKTHCKVANDVLANIEFPYPVAE
                     VIYQHHERLDGSGYPRGLRGNEIALEARIIGVADVIDAMASYRPYRPALGVDAAIEEI
                     VKYRGITYDPDVVDACLTVYKKTARTLIETNHRTVN"
     gene            complement(20247..23060)
                     /gene="uvrA"
                     /locus_tag="PLG98_01720"
     CDS             complement(20247..23060)
                     /gene="uvrA"
                     /locus_tag="PLG98_01720"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOE31996.1"
                     /translation="MDAIKIRGAAQHNLKNISLDIPRDQFVIITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFIGQMDKPNVESIDGLSPAIAIEQRTASHNPRSTVGTVT
                     EIYDYFRLLYAAIGVCHCYRCGREIKSQTIDGILENVLALPENTPFSVLAPMVRGKKG
                     EFQKELKKLQKEGFARVRIDGENRDLAEDISLEKNKRHDIDLVIDRLVLKNGIRKRLR
                     DSLEIASKHAEGLVRIVTAGGEEFLFSEKYACPDCGISLPALTPRMFSFNNPYGACPA
                     CNGLGSSMRFTEYLIVPDAGLSLRDGAIAPWAGRHSLYYYSILDALSSHYGFDVNTPF
                     NKLPEKIRKVLLYGSGSETIKFYHDRGNKRYFVHRPFEGAIRQLERRWRETASTAIRN
                     DLMRYIDLSACETCHGARLKEEILAVTVMGKNIYELCKMSIRDCHSFFERIELSPQEL
                     NITERIIREIKSRLQFLLNVGMDYLNLERATSTLSGGESQRIRLATQIGSGLTGVLYV
                     LDEPTVGLHQKDNLRLIDTLKKLREMGNTVLVVEHDADMIMACDHIVDIGPGAGTEGG
                     EIIFQGTPVQILNSETSLTGRYLSKKESINLPSEKRPLTGRHIILEGASENNLKNIDI
                     KIPVGVFTAVTGVSGSGKSTMVIETLYKVLARRLNQYNGSMGKIKRVVDLGGIERVIM
                     INQHPIGRTPRSNPATYTGVFSFIRDLFTNLPESRMRGYKPGRFSFNVKGGRCEACEG
                     NGLIKIEMHFLPDVYVTCEACHGKRFNRDTLDIKYKDKSIADVLDMTVNQALTFFSNI
                     PSIRSHLQLLGDVGLGYIHLGQSATTLSGGEAQRIKLARELSKKVNSNTLYILDEPTI
                     GLHFADIQKLLDVLMRLVNMGNTMVVIEHNLDVIKCADYVIDLGPEGGSGGGRIISSG
                     TVEKVARDEKSVTGKFLRDVITA"
     gene            complement(23074..24048)
                     /locus_tag="PLG98_01725"
     CDS             complement(23074..24048)
                     /locus_tag="PLG98_01725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE31997.1"
                     /translation="MKFYGKYVFALLGIMLLCPLLHAEVVDKIIAVVNDEVITRLELN
                     AAFEPYRKNIENTYQGGDKDQFVLHAREEFLNRLIDNILIEQEAKKTGISASVREEDV
                     MGVLQDMLTKQNISMQDFLKNLAKDGSSIEEVKKEIRSQMIRMRLLRREVKDKIIVTD
                     EEIGAYYNQHRNDYEGKESVRMKQLLLLLPSGANAETKGKIRKEALHLRERIMAGESF
                     DLLVSTYSQGPAAAQGGDVGFIEKGTIIPEVEAVAFSLAVDQVSNVIESDAGFHIIKV
                     VDKRGAGLKPVAVVREEIKAKIEDEKLDKKFEEWISAIRAKSHIEIKN"
     gene            complement(24070..25077)
                     /locus_tag="PLG98_01730"
     CDS             complement(24070..25077)
                     /locus_tag="PLG98_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOE31998.1"
                     /translation="MMIPTRWFSKFYSLAGKSKKVSPVRCGLLCLLLPGLMIACDKME
                     SFSRPHVATVNGAKIYLDEYQSLLNEKARMLSGDFFRQPAYVKKFEDEVLDAMIGEKI
                     MLLRARELNLSVSDAELEAKIKEIEKDYGNEFTGLFARQNINYEKWKEDFRKEILLQK
                     LIAADVHSKIIVSETEIEDYFKKHRHIYKRDLSARVSQIVVRDMQTAEQAMQRLKSKE
                     EFARVASEMSIGPEASRGGDLGFITKRVMPEPLDETIFKIPVQTISPIVQSSYGFHII
                     KVMEVRQAKERILPEAREEVIADIRLLKEKTAFAEWLEALKKKAVVQKKADIKINTSY
                     R"
     gene            complement(25101..28640)
                     /gene="mfd"
                     /locus_tag="PLG98_01735"
     CDS             complement(25101..28640)
                     /gene="mfd"
                     /locus_tag="PLG98_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOE31999.1"
                     /translation="MITRKNPEDMESFRLLLKQIDDGMMRIAVQGLAGAARSFLISLL
                     YKKRQRPVLVVCPEEKEAVAFARDLSLFLGEEHVFHYPALDFLTIDMFALQRDEELSR
                     LEVLANLQIQHKIIVVASANALMQKVMPVAEFSKHLRIIAPGDTINRDEFAAQLVAQC
                     YKRESLVQEKGEFSIRGNIIDIFPPTEKNPLRLEMLGDEIESIRTFNTSSQRSIGPLP
                     AFVLSPAGEAVMDHSTLELAVRNIRRRADELSFSRDIRSRLVAAVQEGLTNAFNPIFL
                     PLFYESYDTGSGLSDNTLSGLLEYLSRNTLVVLDDPLAVGHAMFNAGLAMDKLLFKAK
                     SGGKFYLEKENTYFPPEAVTAGLDGFLQVALAGLEIESERYHVPVVFESDRFTCTGEP
                     LAGEIREDATLRLTVEKIKSWFAGDMLVFLFCPLPEDVQRMKGLLSSYDLPAETLSGA
                     EPVLDSLEGWKGDARLILFVGDISSGFVMPAIKLAVLSEEEIFVKKARRLRSRPVRTG
                     YFIKSFGDLQEGDFVVHTDFGIGIYRGLKKIAVRKIENDFLVIEYSEGDKLYIPVNAL
                     EKIQRYVGPDGYVPKIDRMGGTSWEAVKERVKKSVRDYAEELVSIYAARETLERKSFA
                     PPDRIYEEFCSTFEFEETPDQVKAIEDIHQDMDDVKPMDRLICGDAGFGKTEVAIRSA
                     FRAVMDGKQVAVLTPTTILAEQHYQTFTRRFEDFPVRVGLLNRFKKPAEQKKIVEELK
                     NQTIDIVVGTHRLLQKDVAFQDLGLVIIDEEQRFGVAHKEKLKKMRTLVDVLTLSATP
                     IPRTLHLSLVGIRDLSVINTPPKDRVSIKTYVMEFDEDAIKTAVEKELARQGQVFFVH
                     DRVRSIYAIARLVQRLVPHAKVGVVHGQMKPAEIEKTMAGFIRQENNVLVCTTIIGSG
                     LDISTANTIIINRAEKFGLAQLYQIRGRVGRSSREAFAYLLLPQGAILSRAAMKRLQV
                     IKEFSDPGSGFRIAYNDLEIRGGGSVLGMSQSGHISAVGYELYTELIEKTIREVKGEK
                     TEEDWMPEIQLGISAFIPEDYVPDVHQRLILYKRISLAANDEDIRAIENELLDCYGEM
                     PRVADNLLRVISIRNVLKPLKGKKMGYDGKYLYIFFRENSPVDPGKIIALYRKKIKEL
                     RFTPDHKLFVPAPAAADMDILVQADMLLKMLAQ"
     gene            28873..30111
                     /locus_tag="PLG98_01740"
     CDS             28873..30111
                     /locus_tag="PLG98_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOE32000.1"
                     /translation="MIPVEKALKTILVNFQPLGLETVHLLKARGRVIGENIMSPRNIP
                     SADNSAMDGYAVRCADTRGAGKTTPVKLKIIEDIPAGKFPRKNIGKNETARIMTGAVI
                     PPGADAVIRQEDTRKDGRTVIIFASAARGENIRFAGEDVRKGESVIPKNSLLRPAHIG
                     MLAALGKSSLKVYQKPRVAIMSTGDELVSIRTNPPPGKIVNSNSYSLAAQVAECGAIP
                     LILAIARDKKSDLEKKFKKALNADVIISSGGVSVGDFDFVKNVMGEIGNAMHFWQVAM
                     RPGKPLAFGAIEGVPLFGLPGNPVSAMVSFEQFVRPSLLKMQGHKKIFRQTVTAICHE
                     EFEKAAGFRHFIRAVVRKEKDKYIAVTTGDQGSGILKSMVTANGLIVMGENETRIKKN
                     SQVTVQLLDDSLSWSDALQL"
     gene            30192..30289
                     /locus_tag="PLG98_01745"
     tRNA            30192..30289
                     /locus_tag="PLG98_01745"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30227..30229,aa:Sec,seq:tca)
     gene            30433..31101
                     /locus_tag="PLG98_01750"
     CDS             30433..31101
                     /locus_tag="PLG98_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOE32001.1"
                     /translation="MDLIEAIQHRRSIRAFTDQPVPKETIMEILNIACRAPSAMNTQP
                     WEFIVVSGEPLNKLRAAIVDKLTSGAPITPDHQVVGWPDKSVYKDRQITLAKQLFKAM
                     DIAREDKEKRAWWMERGFRFFDAPAAIIIVSDKILGDHASLMDLGAVMQNICLTALHF
                     GVGTCIEDQGVMYPEVVRHCTGISSNKRLMIAIAVGYPDETFPANHVRSEREPAENLT
                     AWVE"
     gene            31199..31768
                     /locus_tag="PLG98_01755"
     CDS             31199..31768
                     /locus_tag="PLG98_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32002.1"
                     /translation="MSETTSDWFNKAQALSDGEKFSDPQKAIEYLNNAIRLQPNDAEA
                     YYNRGIAYDNLGRHQQAIKDYSQTVRLKPDYAEAFHNRGTLYNTTGQYQLAIADFNES
                     IRINPGEADNYIGLGFAYDQLGQHQQAITEYSKAIALKPDNARAYNNRGADYLSLGNH
                     QYGCLDAKKACELGNCKLHKIAEKQGVCR"
     gene            32019..32216
                     /locus_tag="PLG98_01760"
     CDS             32019..32216
                     /locus_tag="PLG98_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE32003.1"
                     /translation="MADNMKKNHVKKFRETIPLSISELARRAGLTPQTISKMEKGLST
                     RKNSELKVAKALQRPHEEVFP"
     gene            32313..32540
                     /locus_tag="PLG98_01765"
     CDS             32313..32540
                     /locus_tag="PLG98_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOE32004.1"
                     /translation="MSLLITFGNRGLVDNYLMSKRLAELKSQNSAITAENNALKKKII
                     LLRNDINYIEMIARNELGMVKKGDIVYRWAK"
     gene            32576..33658
                     /gene="pilM"
                     /locus_tag="PLG98_01770"
     CDS             32576..33658
                     /gene="pilM"
                     /locus_tag="PLG98_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOE32005.1"
                     /translation="MVDLKEIFSGKKSLVGLDIGSSSVKLAEIISNAKGYYLNRFHEV
                     PLPKGIIIDGILADAPVLSSKIKELFKIAHCGRKGVVTSLAGNSVIIKKVTLEQMNET
                     ELRELIHDEAGKYLPFDNMDDVNYDFQILGENNYNPNQMDIVIVAAKKEIVSSYLEAV
                     TAAGQNVEIVDVAPFVLETLYEVNYEFDNEEIVVLINIGASTTGINVVKGGMSVFTRD
                     FALAGNSITESIQEKYQIPFEEAEKLKIEGFPEGSENDNLDLKNNILEFAQPICMEIE
                     RSIDYFRSTFGGEDIKQVLLAGGSSRIPNITKHLSELLNVKTEIMDPFLKINYNKRKI
                     DVKNLDSIKPIAATAIGLGLRKIGDK"
     gene            33655..34203
                     /locus_tag="PLG98_01775"
     CDS             33655..34203
                     /locus_tag="PLG98_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOE32006.1"
                     /translation="MIKINLLPYREKAKKDTFLRQIFIVAGSVVLFILLLAWVTIWIK
                     SSISNLETQIKDSEALITDLNKKIGDLEKFKKDKKELEQKIGVIATLEENRLTPLKTL
                     DNLAMLVPPKDIWLVRLTQKGNSLNIEGMGRDNIAVANFMKSIEQFEPVQSVDLVSSK
                     KTEISGNAVQQFNFACILKKGF"
     gene            34207..34929
                     /gene="pilO"
                     /locus_tag="PLG98_01780"
     CDS             34207..34929
                     /gene="pilO"
                     /locus_tag="PLG98_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOE32007.1"
                     /translation="MAIDINTIKKIPPKTKVLIVIVGFLLIGYFYWFYFLSSAMEKRT
                     TLNTQLTEMQNKINEQKKIAMQLNKYKADVEALQASYKIALQKLPDQREIPSLFHSVA
                     SAGRDAGVEFVLFEPKASVPKSLESQGPAASKTAALLKPSDQRDQKPADSSEKKDGAA
                     PSTEPFYEEIPVGISVLGSYQNILYFFDKVSKLPRIVNVSDITIGDRKEIKDKGHLVT
                     GSCTIKTYMFIDKKEKTSEKTQ"
     gene            34926..35615
                     /locus_tag="PLG98_01785"
     CDS             34926..35615
                     /locus_tag="PLG98_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOE32008.1"
                     /translation="MKKIFLIFINCSLILLTTAFFAFAAEAQNTASPAPKSVAPVKGK
                     VQPAPAGTVVPGPARTALSPAAAKTPVPAPAKQAPAVSPAQQTTVSSAANATATPTVQ
                     TPMPAPTVPNQADTYSYNPAGKPDPFRPFIVIETVKKTKPEEKKIKPSIFPLQRAEAR
                     SYKVVGIVGNEDHRVAIAEDASKKFYPLLVGTRIGLHNGKVVDILPDRVIVEEYENNK
                     GNKVILKLHKN"
     gene            35630..37351
                     /locus_tag="PLG98_01790"
     CDS             35630..37351
                     /locus_tag="PLG98_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HOE32009.1"
                     /translation="MKKYFPKIFAAFVIITWIFTCIMGNSFAAFNASEENTAGITMLK
                     SIMLAKLPGKERIHLVVSKQPLIDVKSKDNGSYLIRLEDTDIPESLCRPLGEGELNNI
                     ISIVPSRQQLQGKNWVYLTIYPHEAVPYSIRQEGENVLIDFNVSGIEERKARTISKAS
                     ASKKPLAKVSAEPEKTDDFKKEDLDVSVKRSPAKKYMDRIVSLDFQDADIKSVLRLMA
                     EYGNVSIVTSEEVKGNITLSMKNVPWTKALETILEVNGLTKKQEDNVIIVTSMERSKR
                     EETAREEVRAREQKMMAEKGLLKQVLIEAKIVEATEEFVRNLGVQWGFGTVQRQHIGS
                     SQYGLGASGGSNVATGNQQALTYPSQIGIRDADGNPLAMAAVNLPTSLASPAIGLIFG
                     GASGFLETQLAALETNSTGKIISAPKVVTMEGIKAIIKQGDEVPYITPASGTSPATVS
                     FKEALLKLEVTPKLTDEGKISMDIKASNDTPDWSKAALNPQGNPPIVKSEVESKVVIN
                     DGDTVVVGGILRSTESKAVSGWPWLQKIPVLGWLFKTDSTKRDKRQLLIFVTPKILNN
                     DFTAEGT"
     gene            37554..38213
                     /locus_tag="PLG98_01795"
     CDS             37554..38213
                     /locus_tag="PLG98_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32010.1"
                     /translation="MNKGISFLQIGQFNSALKELLEAEKYYSRNPKVHYYLGIAYHGK
                     GMREQAIEEFEKAVSLDKHYSEAHNYLGTLYSEIQLYDKAIREFEKALGNHLYDTPSM
                     ALYNMAWAYYSKKDYKTALDKYQDVLRTDPMTGLRPQVEKNIGLIHYEQNNDMEAIRH
                     LKKSTELDPSLVDAYFILGQSYLRNKDSKNARRAFENVVKLSPDSSFGLKARNYLQSL
                     Q"
     gene            38311..38991
                     /locus_tag="PLG98_01800"
     CDS             38311..38991
                     /locus_tag="PLG98_01800"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32011.1"
                     /translation="MENSRFDKKQNLLLRKAEAFLREGNFGEALLIAENHLLDCPADA
                     DALGICCEALINMGRLEDMRNILLQTEECISALNLVCERSGDACREKGFHQEAAACYE
                     RFISLRPETEKASEIIEKMTLLDQEDHPTVPEKGKRNIPAQDFFTVTLAQLYIDQGHL
                     QNAKTILDEILKKDPDNRQALSMLNNLISAPAASGTVNGNTKKNDELIKILSSWLKNI
                     ERLKMNAA"
     gene            38981..40066
                     /locus_tag="PLG98_01805"
     CDS             38981..40066
                     /locus_tag="PLG98_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOE32012.1"
                     /translation="MPLNNKKMPQRLSGILEKMSVCKIDGLIICNMSTVRYLCGFTGS
                     EGLLFVGPKKPILFVDGRYTTQAGMETEGIKIIECADRIEAVALMTRQLKLKHIGFEA
                     DFVTWSLHNKLTAQAPETKFIPWTDELKTLRAIKDDDEIMIMKKAAEISSAAIQSVVN
                     RITPGWTEKATAWQLEIAARENGADGTAFETIVASGENSSLPHAKPGKRKIRKGDFVV
                     IDFGVRYQGYCSDETCTLAFGKLTDRQKNAYQVVKDAHDRALELLRADIPGAAIDQHV
                     RSVFGKNYVRYFTHGTGHGVGLEVHEAPRLSSLSDDILKPRMVFTVEPGLYLPGRWGV
                     RIEDTVLLKENSCEKLTKMDKRLTIIE"
     gene            40086..40481
                     /gene="gcvH"
                     /locus_tag="PLG98_01810"
     CDS             40086..40481
                     /gene="gcvH"
                     /locus_tag="PLG98_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOE32013.1"
                     /translation="MKDFPEDIIYSCEHIWVKVDGNMATIGITDYAQERLGDILSVEF
                     IETDTYVERDETFGSIESVKEVVDLISPVSGTVVVVNEDILEDVSIINRDPYDTGWLV
                     VIEMDNLEQLEDLLAASNYHDFIAQEESE"
     gene            40539..41315
                     /locus_tag="PLG98_01815"
     CDS             40539..41315
                     /locus_tag="PLG98_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOE32014.1"
                     /translation="MAIDEAIFRGVITSKTESTIRFYGSEPEAVSIGYFQDAKKDLNL
                     GQCRHDGIDVVRRITGGRAVFHCNEITYSVTAGSGERIFPDNISGTYRVISKCLVRGL
                     GNLGIDARLAETGDERNQGEKVACCFSTSAENEILVNGRKICGSAQVRRRGGFLQHGL
                     LFYRFDPEGTANLLLSSRSPEQIEKLKQSVTSINEYLCQPLTAEEICETLKKSFCDEL
                     GIHLGKGSLTPAEEKLKNELMKKYTDGLWNIEREKYFKAV"
     gene            41322..42386
                     /gene="hisC"
                     /locus_tag="PLG98_01820"
     CDS             41322..42386
                     /gene="hisC"
                     /locus_tag="PLG98_01820"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOE32015.1"
                     /translation="MIIRNLIAKAILTQNGYSAPEITDAIKLDANENPFTLQEPLKRR
                     LADTLRSIDFNRYPAAGAPDLRERFADYYGVKKNMIMPGNGSDELIQILCMAFRGKIK
                     GVLVPSPTFSMYKIIAVNTGNKVVDVPLDQKFDLDVDAIAARIKNNFPALIFLSSPNS
                     PTGNLFSRERIEAVIRKAPGVVVIDEAYSAFAGQSLLPLLKKYKNVVFLKTLSKLGMA
                     SIRLGFLIGNPDLIDQLDKVRLPYNVNALSQKAAGFFLDHEGEFIKQIKEIIQRRQEL
                     YREIKKIKWIVPYPSRANFIFFSCAFDANRIYKDLVSQGIIVKTLNLAPRMPDGIRVT
                     VGSKKENQAFLDALKNITPE"
     gene            42798..42989
                     /gene="rpsU"
                     /locus_tag="PLG98_01825"
     CDS             42798..42989
                     /gene="rpsU"
                     /locus_tag="PLG98_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOE32016.1"
                     /translation="MEVKVIDNDVEKALKILKNKLSKSGLFKELKVRRAYEKPSVKQK
                     RKTIEAQRRLAKVQRRRRY"
     gene            43047..43700
                     /locus_tag="PLG98_01830"
     CDS             43047..43700
                     /locus_tag="PLG98_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOE32017.1"
                     /translation="MDEKQYVIDALSVNEAWRVFRIMAEFVEGIETLSNVDNAVTIFG
                     SARVKPDDIYYQKTEKLAKLLVGERFNVITGGGPGIMEAANKGAAEAGGKSVGLNIKL
                     PFEQKPNDYANIHIDYKYFFVRKVMFVKYAVAYVIMPGGFGTMDELFEALTLIQTKKI
                     KSFPLILMGSEYWKGLIDWLNNAMLAEDKILPVDMELIQIVDEPEDVVKLIKKYVIV"
     gene            43725..45065
                     /gene="holA"
                     /locus_tag="PLG98_01835"
     CDS             43725..45065
                     /gene="holA"
                     /locus_tag="PLG98_01835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOE32018.1"
                     /translation="MEKTLTNLKKGIVAPCYLLYGEEEYLIKEALNKMIAVLVPEADR
                     DFSLFYLDGENTEINSLMDHLLTPSLLGDTKVIVVQDTTIFTSRDDLAKLAAKIRTTI
                     NENPTKAARYFLTFLNIAGFSLEDLYGTGWQKITDEQWSGLVKGDAGDEREKWLPRIL
                     DICQTAGLTDASTSKTEKLEEVLKAGLPAGNCLIFTAEAVDKRKSLYKIVANIGVVNY
                     FSPVKKEYARKEILQKEAQKLLDGCGKKMAPAAWIALGKKTGFDFRNSLSELEKLISF
                     VGERALIDKEDVEEAVGRTREEEIFALTNALSEKNQLAAISTLNHLLDQGIHQLMIMT
                     MLSREIRLLLQARVMVDSGKLPKFNQSMEYGWFQNTVYPVFSALNPTGRKNDILILNQ
                     HPFSVFNALRNCVRFTTSRLTDLLDELLSLERSMKTSASNPQLLLENFLIKFCA"
     gene            complement(45157..46326)
                     /locus_tag="PLG98_01840"
     CDS             complement(45157..46326)
                     /locus_tag="PLG98_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOE32019.1"
                     /translation="MKKFKVKKTIKEINEKIKEGRAVVVTAEEMIDVVERHGDVEAAR
                     KIDVVTTGTFGPMCSSGAFLNFGHTSPKIRASRVWLNDVAAYGGIAAVDCYIGATEVA
                     ENDPLNSVYPGEFNYGGGHVIEDLVSGNFVKLRAEGYGTDCYPARKFERNITIKDLRD
                     AILFNPRNAYQNYNCAVNLSDKTIYTYMGMLRPQMANAAYSTSGQLSPLFNDPYYRTI
                     GIGTRIFLGGAQGFVAWPGTQHNPKVLRGSNGVPREGAGTIAVIGNLKEMSADWLTGA
                     SILGYGVSLFVGIGIPIPVLDEEMARYTAVRDEDIYTQVYDYSMDYPKGSPKPLAEVS
                     YKDLRSGTIVLEGKKVITAPLSSYFKARQIAYTLKEWIECGRFILGEPQQNLPSL"
     gene            complement(46405..46848)
                     /gene="dut"
                     /locus_tag="PLG98_01845"
     CDS             complement(46405..46848)
                     /gene="dut"
                     /locus_tag="PLG98_01845"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOE32020.1"
                     /translation="MKKVKVCIQRLPGNDDIPLPQYMTDQAAGMDIFSAVAEEEIILP
                     GQRKKIATGIAVALPDGYEAQIRPRSGLALKNGVTLLNTPGTIDADYRGEIGLIVINH
                     GEEPFIVTRGMRLAQMVVQRVCRVDWEETETLAKTVRGSGGFGHT"
     gene            complement(46973..49093)
                     /locus_tag="PLG98_01850"
     CDS             complement(46973..49093)
                     /locus_tag="PLG98_01850"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOE32021.1"
                     /translation="MSNVFSADFAGRNISLKANYVAAQADGAVLVYYGDTVVLVTAVS
                     LKSTREGVDFLPLTVDYQEMTFAAGKIPGGFFKREGRPNEREILTSRVIDRAIRPLFP
                     KGYFSETQLVATLLSVDKENDPDVASLIGASAALGISDIPFKGPIAGVRVGRINGEFV
                     ANMCPEKMKESEMSLFLVGRKVTPGTSGRPYDVELVMMEGEAKEVPEDIIVDAIKFGL
                     ESIRPVIDLQDQIQASIGKVKRPVEEPVPDEELVARVREEALPGLKEGYAMPRKLERY
                     SKLGDVRAEVIKNIGGDDAVLSKKVAGIIENLESRILRDMIIQDKKRIDGRSSVDIRP
                     ISSEAGVLPRAHGSALFSRGETQALAALTLGTSADEQRMDYVGGEEIRTFLLHYNFPP
                     FSVGEAKMLRSPGRREIGHGALARKALVPVLPSPEEFPYTIRIVSEILSSNGSSSMAT
                     VCGGILCLMDGGVPVKDIVAGIAMGLLKEGNEIVILSDILGDEDHAGDMDFKVCGTEK
                     GVTAMQMDIKIDGLTEDILRKALAQAREGRMHIIGKIRETLAAPRGDISQYAPRITTV
                     KVKEDQVRTVIGSGGKNIRQIISETGVTIDVEDDGTVTIASADAEAAARAVAMVKWLT
                     EEAEIGKIYRGTVKKIVDFGAFVEILPGTEGLLHISQIAKERIAKVTDVLQEGDEVMV
                     KVLEIDKSGKIRLSRKEALGAEVK"
     gene            complement(49229..49495)
                     /gene="rpsO"
                     /locus_tag="PLG98_01855"
     CDS             complement(49229..49495)
                     /gene="rpsO"
                     /locus_tag="PLG98_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOE32022.1"
                     /translation="MLSSEKRKEVIDNYRLHEKDTGSPEVQIALLSARIEYLTEHFKA
                     HKKDHHSRRGLLKLVGQRRRLLNYIKENDIERYRTVIKRLNLRK"
     gene            complement(49553..50488)
                     /gene="truB"
                     /locus_tag="PLG98_01860"
     CDS             complement(49553..50488)
                     /gene="truB"
                     /locus_tag="PLG98_01860"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOE32023.1"
                     /translation="MMNGVIVIDKPAGKTSHDVVLDVRRALGIKKAGHTGTLDPLATG
                     VLPVCVNEATKLAGFLAGDKKEYRATMLLGVTTDTLDIEGNITGQSDKVVSGEEIRSV
                     MMRMIGTINQVPPAYSAVKHCGKPLYKWARKGVFPDLKPREIKIFSLLVENISFPSVK
                     FRVACSKGTYIRSLCADIGEALGTGACLSGLRRLQSGIFSEAMAVNLNDYKFPEAKEK
                     LAEKILPMAELLPLLATIELEDQCAVKLRNGWQPSVEMLKEHDLPLLKAGDMIKFVSR
                     GNLLAVAEMLAPVSELAAFDGKRQAARILRVFNQP"
     gene            complement(50494..51444)
                     /locus_tag="PLG98_01865"
     CDS             complement(50494..51444)
                     /locus_tag="PLG98_01865"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOE32024.1"
                     /translation="MIHDIIQAIHEGQNFLITAHVRLDGDALGSELAVYLMLKELGKK
                     AVVYNKDRTPERYQFLPATHNISHTLTNIEQYDTCIVLDCSDLSRVGDEAENIRKIKT
                     LINIDHHVSNNGFCALKMLDAKASSTGELVFRLMQDMRFKMSRDICTNLYAAIITDTG
                     SFRYSSTTKETFMAAGVLVGEGASPQRIAENIYESDSPARLKLMARALSTLSLDLESK
                     VGSMVVTQKDLLETGAAWEHTEGFVDIPRTVKGIEVSVLYTQRGEKNFKLSLRSKAKV
                     DVEKVAKRFGGGGHIHASSCWMDGDIETIKSQIIQAVREL"
     gene            complement(51434..51796)
                     /gene="rbfA"
                     /locus_tag="PLG98_01870"
     CDS             complement(51434..51796)
                     /gene="rbfA"
                     /locus_tag="PLG98_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOE32025.1"
                     /translation="MNFKRADRVAELIRADMSEIIMKDVKDPRLHSVTITAVKVSDDL
                     RNAKIYFVEMGKDECSVEIKAGLAQASGFIRRELGKKLQLRFVPELMFIHDTSFGYGN
                     RIEKLLAQIHSQENENDS"
     gene            complement(51786..52124)
                     /locus_tag="PLG98_01875"
     CDS             complement(51786..52124)
                     /locus_tag="PLG98_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOE32026.1"
                     /translation="MVVGYGIINLRIPESGSLKSKRSVLNRILKRAQNEFNVSIAETG
                     HLDNYKLAEISFAVVGNDSRYINGKADHLLRFIDHLQIAEIVNCKMEIMVVSSFLETA
                     DGDIQKYDEF"
     gene            complement(52194..54827)
                     /gene="infB"
                     /locus_tag="PLG98_01880"
     CDS             complement(52194..54827)
                     /gene="infB"
                     /locus_tag="PLG98_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOE32027.1"
                     /translation="MPKKRVYELAKELGMESKVLIARLEKIGISVKSASASLEEADAE
                     RAKKELTAGELREVVEQRIKTTVIRRRTVVTAVEKPPEEVPLEEAKPPEKEPAEDKLK
                     KAPPGKTEKVKKEKPGEEPAVPAETVSEKAVKEVVPPAAVGPAAAETKASLEQKAAPL
                     EKSAEEKAVKPETKEAPVAPVKPTIITKHKIIRPETGKAVPPKVPAAKPAEKISQPSA
                     EKPKKKGKYQVEVLIEEEKKIPTKKILEKKIEKRLKRHEEDQEVVFTKWREGKKAAPT
                     KMKKTEITVPKAIKRRIKIGETITVGELAKRMGIKVSEIINKLMSMGVMATINQAIDY
                     DTASIVAGEFSFQLESADVEFDESVLKAPSTPENLKPRAPVVTIMGHVDHGKTSLLDA
                     IRQTNVSDGETGGITQAIGAYHVHVNGRDIVFLDTPGHEAFTAMRARGAQVTDIVVLI
                     VAADDGVMNQTVEAINHARVAGVPIIVAINKIDKPGADPEKIKQALTEHNLLSEQWGG
                     DTIFCEVSAKKKINIEELLEMILLQADVMELKADPDRPVRGIIIESKLDKGRGPVATV
                     LIQEGTLRESDSFVSKTEFGRVRALINDQGRRIKEAGPSMPVEVIGFSSVPQTGAEFF
                     CVDDEKKARAIADYWTRKAREKELSSLSKITLEQLYQRIKEGAKEFNVIVKADVQGSI
                     EAISDALNKLSTDDIKLKIIHSSTGAISETDVMLASASQAIIIGFNVRPDARVVDVAQ
                     REGVEIKLYDIIYNVIADVRAAMEGLLEPEYREVVQGRAEVRELFKVPKVGTIAGCYV
                     TDGKIMRSNNLKLVRDSVVVFDGKILSLKRFKDDAREVLAGFECGIGIEGFNDIHPGD
                     VIEAYAIETLEKKLEPNADKR"
     gene            complement(54854..56137)
                     /gene="nusA"
                     /locus_tag="PLG98_01885"
     CDS             complement(54854..56137)
                     /gene="nusA"
                     /locus_tag="PLG98_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOE32028.1"
                     /translation="MLPELKRLIEQMGKDRGISKEIIVEALEAAMLTAASKKFGQNVE
                     IEAHYNDETGEVEVFQFKTVVDKVLHPETQISKEEARATLDEGAEPGDSLGMKIETSQ
                     FGRIAVQLAKQIIIQRVKDAECDNIYDEYKDRRGELVNGFVQRFEGGSIVVNLGRAEG
                     IVPPSEQIHREAYKRGERIRAYIVEVKKSPKGLQIILSRTHPSFLKALFEVEVPEISE
                     GLIDIINVAREPGKRGKIAVKSKDKDIDPVGACVGMRGSRVQSVVQELRGEKIDIIPY
                     SEDPAKYVSSALSPAKVNRIFIDDENRTMTVIVPDDQLSLAIGKNGQNVRLAVKLTGW
                     NIDMKSETMEQAQEEGKSGQNDLTKITGIGPAMAEKLFSKGIKSIAMVAATEPELLSQ
                     IPGIGEKTAQQWIETAGKILNEEIAGSKSDSSSEN"
     gene            complement(56211..56684)
                     /gene="rimP"
                     /locus_tag="PLG98_01890"
     CDS             complement(56211..56684)
                     /gene="rimP"
                     /locus_tag="PLG98_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOE32029.1"
                     /translation="MDFDVKEKIRLLAEPVVASEGMELIHVECIKMHTRWIIRLFLDK
                     EGGITLEDCTNISNQLGDIFDIREVVNGSYTLEVSSPGFDRPISRDQDFLKYRNSRVD
                     VKTGIKIDGVKNFHGVLLDYIEENGRKLILVEVSGKVYRIPRQEILKANLADEKK"
BASE COUNT        15193 a        14551 c        13157 g        14090 t
ORIGIN      
        1 acccgcaacc tgctgattac aaatcagctg ctctaccgat tgagctacgc cagccaatcc
       61 catcaaaaaa tataaaattc aataacttgc gcatcagaat gtaaaaaaac acacctacac
      121 cctcaagcga atttcgcaat ctaattttta tagatgtttt tgtcaagtaa atttttttaa
      181 aaacatgcag gtaaatttag tgcgtggaaa aatcccgtat agacaaaaag tttaacattt
      241 tttaaacttt attgaaataa aatccgatct tcctctgaca cgaatattga ttttccagcc
      301 ctgagtccgt ttaatttatc ttaacttcaa tagaatagac ggatttctcc tttacggcaa
      361 gaaaacggaa tctgtctgat gtctcaaagg caaaaccttt tttccccaaa gcaacgatgc
      421 tgttatattt attgacctct ttttcgccgg cctttccatt tacaacggct atttgtttat
      481 tttctgcctg cgcaacataa actatttttg tgctgtccgg actgaaaacg agcgtgtaac
      541 ttcccactcc atcatagtgt ttcccttctt tcccgtcaac gacaacaaac cgtttctgtc
      601 ctgactgggc tgcatacgcc acacgcttgc tgtccgggct gaaaacaaga gacccgttgc
      661 cgatattgtt gtacggtttc ccctcctgac cgtcaatgac ggacgcaaac ttgccattca
      721 cgatctgagc gatataagcc acttttttgc tgtccggact gaaaacgatg gttgatccga
      781 tacgttcata tcctttacct tcctgtccat tgaccacaag aaaacgtttc ttgtccgatt
      841 gcgcttgata cgctatccga ctgctgtccg gactgaagaa aatatcatct ccgatgccgt
      901 cataatattt gccttcctgc tcatcaacga caacctgcca cttaccggct gattttacca
      961 cgtaggctac ctgcttgccg tccggactga aaacaaaatc gcctctgaca tcatcatagc
     1021 gcttgccttc ccgtccatcg acaaccagaa aacgtttttt atccgactga gcctgataag
     1081 ccatccggcg actgtcggga ctgaaaacga tattattgcc gatactgtca taatgagtgc
     1141 cctcttgttg atcgacaacg acaaactgct ttttatctga tagcaccccg taagcgattt
     1201 ttttactgtc cggactgaaa ataatattgg agccgatacc tccctcgtac gatttgcctt
     1261 ccttcccgtc caccacaata aaacaccgct tgcctgtttt ggcggcatag gcaacccgtt
     1321 gactgtccgg actgaaaacg atatttgatc ccactgtgtc atagcgtttc ccttcttgac
     1381 cgtcgattac cacaacctgt ttattgccaa cctgggctcg atatgcagaa cgcttgctgt
     1441 cggggctgaa aacaacattg gaaccgatac cgtcatagcg tttctgcgcc ttgccgtcga
     1501 caataacaaa ctgcttgcct tccacacggg tggcataggc aatacgtttc ccgtcgggac
     1561 ttgccgttaa tacgacagga ataaaatcaa agttaaaaag cagtttttca gaagcagaca
     1621 acgtaagtgc tttttcttcg gcaaaggaat ttaaaccaac tgcaacgaga aaaaaaacag
     1681 ccagcggtaa taaaaaaaat ttcttcatct ttaaatagcc ccttttgtaa ttgatgtaaa
     1741 aaacctgctc aagaacagat ccgtgcttgt agccattgtt ctttacatgt aacgaatgaa
     1801 taaaaattta ttgtgctgtc ctcttagtat atttctttct tacatgcaaa tgtattattg
     1861 acatatctcc agcattaaaa aatcatccgg tggggaaaaa gcatcatccg taacctgtta
     1921 aacgccatct accgctttgg gagtgcattt gttatcagtt ctttatgtca gttttatttt
     1981 aatgaattca aaaaaaattt tgacaagatt ctttttttgc attataaagc ctccaaaatt
     2041 tttggaattg caaaaacgat gacggaaaca gcgaaaaaga atttcatagc aatcaacatt
     2101 ctgcctttac tgctggctct cgctttgttg cgccttttac ctcaggccgc ccgcccatcg
     2161 tttttaggtc atcactagga gaaaaagcaa ctgatcacct aaaaggccgt gggcggaagc
     2221 cacggccttt tttgtttttt aaaaagcggc ataccgcccg atagatggaa actacgcggg
     2281 gaaattacgg tacattgccc acgaaccttc ggttactggg atcattcata tagtcatcag
     2341 aggagaagat attatgaaaa aaaagaaaaa tcgtgtttac atttttgata caaccttaag
     2401 agacggcgaa caatcaccgg gttacagcat gaacacggaa gagaaactga tgcttgcccg
     2461 ccaactggaa aggctgggtg tagacatcat tgaagcggga tttcccatcg catccgaagg
     2521 tgactttgac gccgtcagac aaatcgcgaa ggaaattaaa aaatcccaag tggcgggtct
     2581 tgcccgggcg aataaactcg acattgaccg cgcctggcag gctatcaagg gagccgcaca
     2641 tccccgcatt cacactttta tttcttcttc cgacatccat ctgaagtatc agctcaaaaa
     2701 gagtcgcgag caggtactca aagaagcggt tgccgcggta aaactggcac gttcttatac
     2761 cgataatgta gaattttcgc cgatggatgc cacccgttcg gacaaggatt acctggtcga
     2821 aatggtttcg gcggtgattg acgcgggagc ctgcaccgtc aatatccccg atacggtcgg
     2881 ctatgccacg ccggaagaat tcggcaatct gattgcctat ctcttcgcgc atgtccaaaa
     2941 tatcggcgaa gccatcatcg ccacgcattg ccataatgat ctagggctgg ccgtggccaa
     3001 ttcactggca gccatcagac agggtgcgcg gcaggtagaa tgcaccatca acggtatcgg
     3061 cgagcgggcg ggcaataccg ccatggaaga agtcgtcatg gcactgcaaa cccgcaagga
     3121 tctcttcggc cttcacacga acatcaagac caatcagatt tacaagacct cgcgtcttct
     3181 gactcagatt accggcatac cggtacagcc caacaaagcg attgtgggcg ccaacgcgtt
     3241 cgcccatgaa tccgggatcc atcaggatgg attgatcaag gaaaaaatca cctatgagat
     3301 catgacgccg caatccgtgg gtattagcga cacacatatt gtcctgggaa agcattcggg
     3361 acgcaacgcc atttcacacc acctgaaaaa gatgggatac caactcaatg atgagcagat
     3421 caacaaggtg gcggctcgcg tcaaagaact ggcagatatc aaaaaagata tttacgatga
     3481 agatctcgag gtcattctgt ccgaagaaat ctaccggggc gaagacaaat acagcctgct
     3541 ttatctgaat gtggtcagcg gcaatgtggc cattcccacg gcaaccatgg aaatgcaggt
     3601 gggtcaggac atcgtgcgga aagcgggttt cggcaacggt ccggtcgacg ccaccttcgc
     3661 agccattcgc aacataacca aaaccaacta tccgttgatg aagtacgttg taaacgccat
     3721 caccggagga tcggatgcgc agggcgaggt caccgtgcaa ctcaagcaca gcagttactc
     3781 ggtcgtcgga cgcggcgctc atccggatgt cattgttgcg tccgccaaag cttatatcaa
     3841 cgccttgaat cgcatggaat ttttaaagaa taatattaaa aaggttaaaa ttgttgaata
     3901 atgaattaaa atacgctaaa gaccgtttca ctttttacaa ggagaacccc tcgtgggcaa
     3961 caccattacg gaaaaaatat taatggcgca caccggcctg agaaaaattt caaccggcga
     4021 tctgatcaac gcgagagtgg atattgccct gggcaatgat gtcaccgctc ccatagccat
     4081 caacgcgttc aagcaggcgg gcggggaaaa agtattcaac agacataagg tggctctcgt
     4141 cctggatcac tttacgccca ataaggacat taattccgcc cagcaatgca aagcggtgcg
     4201 cgaatttgcc tcgcaacaga agataacgca tttttatgaa ggcggccttg tcggtgttga
     4261 acatgcctta ttgcctgaac aggggatcgt tctgcccgga gacctggtta tcggtgcgga
     4321 cagtcatacc tgcacgtacg gcgctctggg cgcattcgcc accggcgtcg gcagcaccga
     4381 cctggcggca gccatgctca ccggagaact gtggtttaag gtaccgccct cgatcaaatt
     4441 catcatcttc ggcaaactgc agaaatgggt ctcgggaaaa gatttgattt tgcatatcat
     4501 cggccgcatc ggcgtggacg gtgctttgta taaggccatg gaattcaccg gagagacgat
     4561 tgcccgcctc acaatggccg acagattttc catggccaat atggccatag aagccggcgc
     4621 aaaaaacggt atctttgctc ccgacgaagt gaccgcagaa tacatccgga aaaaggcaaa
     4681 acgacattat acattttatt cttccgaccc cgatgccgtt tatgccgaca ctcttgaaat
     4741 aaacgcggcg accatcgaac cgcaggtggc cttcccgcat ttgccttcca atgtcaaagg
     4801 agtgagcaaa gcaggacggg taaaaatcga ccagtccctg atcgggtcct gcaccaacgg
     4861 acgtatagaa gacctgagaa tcgccgcaaa aattttaaaa aacaggaagg cctcctcgaa
     4921 tgtcagactg atcatcgttc cggcaacccc cgaaatttac cgccaggcgt taaaagaagg
     4981 gctgattgag atttttttga aagcgaacgc cgtcatcagc ccgccttcct gcggcgcctg
     5041 tctgggcgga cacatgggca tcctggcgga aggagaaaaa gcggttgcga caacaaaccg
     5101 gaattttgtc ggtcgcatgg gtcataccaa aagcgaagtg tatctggcca atccggcggt
     5161 tgccgccgct tcagccgtac tgggaagaat tgcctctccg gaagaattgt aagaaagcgc
     5221 acatgcccct tagcttcgca cgtaggataa ttcaacttgc agatattact acgctacgcc
     5281 tgtaatatct gagtttcatt aaaatttaag actcattaca acttacaatc ttctatacac
     5341 attgtttctg aagattgagt ctcgttaaaa aggattttaa catgatattt aaaggaaaag
     5401 tctggaaatt cggagataat attgataccg acgcgatcat tccggcgcgt tatctgacga
     5461 cgtcggatcc tgaagtcctg gcggttcact gtatggagga tgccgacccg gacttcatga
     5521 aaaagatgaa aaagggcgat atgattctgg gcggagaaaa tttcggttgc ggctcgtcgc
     5581 gcgaacacgc gcccatttcc ataaaagcgg ccggcgttcc ctgtgtcgtc gcgaaaagtt
     5641 ttgcccgcat tttttaccgg aatgccttca acatggggct gcccatattc gaatcgaaag
     5701 atattgtcga tgccgtgaaa gaagggaatg aaatcaccgt ggacagtgca cgcggcatca
     5761 taaccataaa cggcacggat aaatttttta aaatcaaccc cattccgccg ttcatggaag
     5821 aacttatcgc caatggcggt ctgatgcaac acatcgctgg gaaaagaaaa aaaaatgaaa
     5881 aataataatt ttaaaatcgc tgtctttgcc ggcgacggca tcggacagga aattataaac
     5941 gaagccctga aggttttaaa aattatttcc ggcaaaaaaa acgtcgactt tcaaatcaca
     6001 gaaggacttg tcggcggaac ggcgtatgac cgttatggaa cgcctctgcc ggaggagagt
     6061 ctgaaactgg ccctgaaaag cgacgcggtc ttactgggag cggtcggcgg tccccagtgg
     6121 gaaaatctcg cttacagtgt ccggccggag agggcgttgc tgggcttgcg cagcagcctg
     6181 ggcatttacg ccaacctgag gccgatcgtt gtttttgaag aactcctgga tgcgtcgcca
     6241 ctgaaaaaag acatcatccg cggcatcgat attatcattg tgcgcgaatt gacaggcgat
     6301 gtctatttcg gcaaaccgcg cggcattaag acatcgaaaa acggaaaacg caaaggcgtc
     6361 aacacgatga tttattcaga agatgaaatt cgtcgcatcg ccgtccgcgc tttcgaaata
     6421 gcccgtacac gcagaaagag cctcctctcc gtagataaag ccaatatctt ggaaacgatg
     6481 gaactctggc ggaacgtggt caccgacgtc agccgcaatt accccgatgt cacgctgaga
     6541 cacatgtatg tcgacaattg cgccctgcag ctgatccgtg atccgtctca gttcgacgtt
     6601 atcgtcacca cgaacctgtt cggcgatatc ctgagcgatg aagccgccat gctcaccgga
     6661 tccatcggta tgcttccgtc agccagtctg ggggaaaaaa cagccttgta cgaacccatt
     6721 cacgggtcgg cgccggacat tgcgggacaa aacatcgcca atccgctggc cgctattttg
     6781 tcggtggcac tgatgctgcg gcattcttta tcaatgccgg cggaagccgc cctgatagaa
     6841 aatgccgtta cggaaacctt acaggaaggc ctccggacac gcgacattga tcaaccgggc
     6901 aaaaaaattg tcagcacttc agcaatggga gacgcggtag ccgggaatct taacaaacgg
     6961 ttggccggga ttcgccctta aaggccggct tcgcgttgag caaaagggga aagcatggaa
     7021 tccgtcattt ttctggaaaa aatttcgatt gaacctcccc gcgaaaaaat atatcagcgg
     7081 ctcggtttta aaaagagaat aaccgccgtc tcgccggatc agcaaaagca aaccgacctt
     7141 ttcattcgcg aagccgcgtc ccttattgtc ctcaagggga tgtacctgcg gctggccgtc
     7201 acaaagaacg acgggaaaca aattttttta tcgaacaacg tacatttcag cagcgagcat
     7261 ctgtccattt tccttcgcga atgccgggag gccgttttca tgggagcgac tgcggggcat
     7321 gccgtcatgg acgccatcaa ggcaaaaacc cggcaggacg atctggccgc cgcggtcgtt
     7381 tatgatgcca ccgccagcga aatgacggac gccgcactgg actggatcat gaattatctg
     7441 aatcaccttc tgatgcggga aggccaaacg cttttgccac gccgtttttc cgccggctac
     7501 ggtgactttc atctggaaaa ccagggaatc atttataaac agctacacat gcagaacgcc
     7561 ggcgtaaaaa tcaatcccaa ttttattctc ataccggaaa aatccgtcac cgccattggc
     7621 ggaatctgcc gttaaaatgc cacgcacaac aaaaagttaa aggttcttcc ttacagtgcc
     7681 aagattgatt ctccgacgat tacacaattg acaaacagac atgaaaagct tatgctgttt
     7741 accagtaggg gatgcctcat caaggctgat aacaatcagc aaaacaaaaa gcaactgata
     7801 gaattctaac cagtccgatc cttactccag tttcacccag gggaacaggt gtatgaaaaa
     7861 aaataatgcc cctccaaccg gcaatccgca gccccttcag gttttaatgc tgaacgattc
     7921 ggacaaggac gtgtcgcgca tcgtgagcga acttcaaaaa ggcggatacc atccggttta
     7981 cgaacggata gaaaccgctg ccgcgatgcg gcaagctctc cgggacaaac catgggatat
     8041 cattctttgt gacagcaatt acctcaaatt cggagctgtc gcggccatgg ctcttctcga
     8101 ggaagaaaaa accgacattc ccctgattgt catctccggc gccagcagca aagataaaac
     8161 tatcgaatgc cgctttcccg ggactcgcaa ttacatcgtc aaggagaact attcccgcct
     8221 atgcccggtc attgccagag agctgaacga aacaaaaatt caggcaaagc acaagcagac
     8281 agaatcccag aaggaagcgg cacaggaaga attacgccgc agcgaggaac ggtatcgtaa
     8341 gattaccacc tgcataccgg atttgatctg gaccatggac ctggcgggtc attttacata
     8401 tgtcaacgaa gtcgtggagc gcacacacgg ctggacggtt gacgaatatc tgaagcttgc
     8461 cctgcaggat atggtcacac cccaacaact ggccaaagat acggcaatga tggcagaaca
     8521 gttggcccgg tcttctgaac ccgatttcga tcgaaatacg acgcttacgt ttgaatcgga
     8581 agagttgcgc aaggacggaa ccacttttct ggcggaagtt accgcttctt tcctgtggtc
     8641 ggatgatggc aagcccattg gcattatagg gattacacgc gatattacgg aacgcaaaaa
     8701 ggcggaagaa gcgttacgga aaagtgaaga actgcacatc aaactggtca atgcaattcc
     8761 ggatgttgtt gttcgcaccg acctggcagg caatattctt tttgcaaatg agaatgcgct
     8821 gcggatcggc ggttacagca aagatgaatt tctgggtcag aatctgctta acttcgttgc
     8881 accggaggat cgcgagagag tcattcgcaa tctgcacctc atgctggaaa gcagactggg
     8941 ccccagagaa tatcatctga tgaccagaga cggacggaaa atcctgtttg aaatcaacgg
     9001 cgatgttttg cgcaaagaag acggaacacc gtttgccgtt gtcaatgtgt gtcgtaatat
     9061 taccgaacgc aagcaaattg aaaaagcgct gagggaaaaa gatgaacgat tgcgcggcat
     9121 cactgaaaat cttccggggg ttatttttca gttttatgca acacagaacg gcaaatacgg
     9181 aataacctac ctcagtgaac cgatcgatga atttgcaaaa atattgtcaa agttcgatac
     9241 ggaccatttg gatacggtgt ttcctgaatt cctttcccgt attcatgaag atgaccggga
     9301 gcgatttctg tcatccatta cccaggcagc ccagacagcc agccgctgga attttgaagg
     9361 tcgcgtcgcc atccaatccg gaaagattat ctggtttcag gggctatccg tgccgacacg
     9421 cctgccggac cgggtcattt tcgatggaat tttattgaac atctcggagc gcaagctggc
     9481 ggaagaactt tcacaccaat cggaggaaaa attctataaa atttttatga ccacgccgga
     9541 ctgcatagcc attgcccgcc tgcatgacgg tctgatcatc gatgtgaatc aaggattcga
     9601 aaagattgtg ggatggaaaa gagaacaggt catcggaacc aaatcgttcg aaccgcccat
     9661 gaacatctgg ctggacaaaa cagagaggaa ggcaatgatc gcggatttga aaacgggcag
     9721 ggatatcctg gatcgggagt ttaaattccg gcgcagggag ggctccgaac gtttcggtat
     9781 ttactccgcc agggcgatca ccatcgacga acaggaatgt gttattttta tcctgcaaga
     9841 tatcacagac cgcaaattaa tggacatcga actgaaacgg actctggaaa gtctgagaaa
     9901 atcggtcggc accaccatcc aggtcatggt gtccgccata gagatgagag atccctacac
     9961 cgcgggtcat cagttgcggg tgacagacat tgcccgggcc attgccgcgg aaatgagatt
    10021 atcccaggat aaaatcgacg gcctccggat ggctggcgtt atccatgaca tcgggaaatt
    10081 atccgtcccg gcggagattc tggcaaaacc gaccaggctg accaaactcg aatactctct
    10141 gatacaggaa cattcgcgaa gcggatatga aatattgaag aatgtcgaat cctcctggcc
    10201 gctggcagaa attgtttatc agcatcacga aaggatggac ggatcggggt atcccagaaa
    10261 cttgaaaggg aatgatattc ttatcgaagc tcgcattctt gccgtggccg acgttgttga
    10321 atccatggcc tctcaccggc cctaccgcgc ttcgttgggg atagaggccg ctctggaaga
    10381 aatcgaaaag aacaaaggca ttctgtatga tgagcatgtg gccgatgttt gtctgatctt
    10441 gtttcgtcat aaaggcttca ttctggcata acatccatca catccgtcaa ataacgaaat
    10501 gaaaattgca gaaaagcagc cattgatgta taagatcacc ggaaactgat tctttgcatt
    10561 tcaggagata gccgtggttg aaattattaa tataagaaat tttttaaaaa agaaaattat
    10621 gattctggac ggcgctaccg gaacagagct ccagaagctg ggcctgccgg caggtgtttg
    10681 cccggaaata tggtgtctgg acaatccgga catcatcggc gctgtccaca aatcgtatca
    10741 gaaagccggt gcacagatta tttatacctg cacctttgga gccaatcgct ataaactcaa
    10801 acagttcggc gcaaagcacg atgtttacgc cgtcaatctg aaactggcac aattggccag
    10861 aaaagcctgc ggcaatcaga cactcgtcgc cggagacatc ggtccgaccg gattgtttgt
    10921 cgaacccttc ggcacgctgg cttttgaaga agcagtggat gcgttcaagg aacaggcccg
    10981 aggattaatt gacggcggat gcgatctgat cgtcatcgaa accatgattg acattcagga
    11041 agcgcgcgcg gcgctgctgg ccgtcaagga acttgcggac gttttcacga ttgcatccat
    11101 gacgtacgaa aaaaacggcc acacgctggg cggaacaccg ccacaaagcg cgctcatcac
    11161 tttgcaaagt ctgggcgcag acgccgtggg ctgcaattgt tcggccggtc cggaacaaat
    11221 gattgatttc atctcggcga tgaaaccgta cgccacggtg ccgcttgtgg ccaagcccaa
    11281 tgccggcatg cctcttctga aagacggtca aaccgttttt gaaatggatc ccgcgacatt
    11341 tgccgatttc agcgtcaagc tggcggctgc aggcgccaat atgctgggcg gctgctgtgg
    11401 aacaacgccc gaacatattc gcgcgctcag gatgtcggtc acgcgtaaga aacccgtttt
    11461 gccgcttaca aaatccgttt gtgcgctcag ctcggcacgc agattcctgg aaattaaaga
    11521 caatgacccg ttgtatatcg tcggggaaag aatcaatccc accggtaaaa aatcgctgca
    11581 gcaggaactc ttggagggca aattatccat catccgtcag atggccctgg atcaggaaaa
    11641 ccagggcgcg gttcttctgg acatcaatgt cggtcagccc ggcattgatg aggttcaaac
    11701 gatccggaac gtgatcagtc tgttgtccac cgtcagcagt ctgccgctgg tcatcgattc
    11761 gtccaggatt gaaaccattg aggccgccct gagactctat ccgggaagaa tgatgattaa
    11821 ttccatctcc ggggaaaaag aaaaactggc caggctcctg cccctggccg cccgatacgg
    11881 cgcaatgttt attttgctgc cgctgaccgg cggggaaatc ccccggacgg cgaaaaagcg
    11941 gcgcgtcgtg atcgacagga tatttcgcaa agcgcaagcg ttcggtttta cgaaaaagga
    12001 tttcgtcgtc gacggcctga ccatggccat tgcgtccgat gccgacgccg cgcgggaaac
    12061 gctcgaaacg attcaatggt gtaaaaacat tttccagtgc aaaacgattc tcggcctttc
    12121 caatgtttcc ttcggcatgc cgggccgtcc ctggatcaac gccgcctttc tggcgatggc
    12181 tcaacactgc gggctgacca tggccattgc caatccggcc agcgtcgagt tcatgaatgt
    12241 taaaaatgcc ggggacgcct tgaccgcccg ggaaagagcc gcgtttcact tcatcaacta
    12301 tttttcgcag cagactcccg ctgtcgcagg actcccttcg acagccgttt cagcgggaga
    12361 aaaaattgtt caggccattc tggaaggcaa caaagacgac atcaccgcat tgcttgacga
    12421 agcaatgaaa tccggcaaaa cggcgtctca actggtcgat gattatatga tcccctccat
    12481 tgtgcgtgtc ggcgatctct acgagaaaaa aatttatttt ctgccgcagt tgatggcggc
    12541 ggcggaagcg atgaaaaaag cgctttcgca ccttgatccg tatttgcaga aagcgacaat
    12601 aacgaccagg ggaaccgttc tgatggcgac ggttcgcggc gatgttcacg acatcgggaa
    12661 gaacatcgtt gcgctgctgt tgagaaacta cggttatcgt gttattgacc tcggcaaaga
    12721 cgtgtccgaa caggctattt tagatgctgc aaaaaaggaa aaaccggatg tcatcggcct
    12781 ttccgcgctg atgaccacca ctatggtcaa catgaaggag gttattgcgc tggcacgcgc
    12841 gcagggaatc cgcacagact tcctgatcgg cggtgcagtg gttacggatc aatacgcaaa
    12901 atccatcggc gcattctatg cccgtgacgg tgttcaggcc gtaaaagttg ttgaaaaaat
    12961 aatcaaaccg taatcgttcg gggaaaaaat gaaacacagg cattccgctc atcaacaaat
    13021 cgctctcatt tgggccttgg ctttttccgt gctgattcat ttttcggcgg cagcggttct
    13081 tatcgggttc gagttgtccg gtggtgcgcc ggcgccgtct gaccgaaatg ttttgaatat
    13141 ggtctgggtg tctttcgaca ataaaaacgc cgacacggga aaagccgtgc aaaccgggca
    13201 gcaaacgata agacagaaaa aaaacaccgg acggatgacg tccgtcgaac ggccggcgag
    13261 ccccgtcggc accgttcagc ttcctttggc gtcccgaacg gctccggctg tcgaaagtac
    13321 attttccgaa tacgaaaacc gccccgaaat aaacaaatca tccgttgagg cacgtcacag
    13381 cggcggcttc gaggcagttg cccaacatcc catgtccgac atcgccattg cctatccgct
    13441 gtaccgggag aatacccctc ccgtttatcc ggaaatggca cgtttgcgcg gatatgaggg
    13501 catcgttctg gtcttcgccg aaattctgcc gaccggacgc gtgggcgacg tcaagatccg
    13561 gaaatcatcc ggatacgcta ttctggatca atctgcaata caggccgtca agccctggaa
    13621 attcgaaccg gcgaagaaag ctggaaatcc attcaccgcc tgggttgaac tgccgatcaa
    13681 gtttatgtta caacatcacc ctcaatcata aatttcatcc gaacggagga accacaatga
    13741 ccattgatca aatgaaggaa cgactgggag aaatcattct cgaaaggtct ttcaaataca
    13801 gcgacaatcc cccttttacg ctcgcctccg gcaggcaaag caattattat tttaactgta
    13861 aaccgacgac cctggacccc gaggggatga atctgatcgg caccatcatt tttgacatgc
    13921 tcaaagatac cgatattacg gccgcaggag gactgaccct gggagccgat cccatcgcca
    13981 atgcgcttgc cgttatctcc taccagaaag gcaaaccgat caaatcgttt atcgtgagaa
    14041 aggacgccaa ggatcacggt actaaaagcg ccattgaagg cagtgtccgg ccgggagaaa
    14101 aagttgccat tatcgacgat gtgatcacga ccggcggatc cacaatcacg gccattgaac
    14161 aggcccgcaa agccggactg tccgttgtca tggtgataac gcttgtcgac cgggaagaag
    14221 ggggccggga aaatatcatg aagcacatgg acaacatcgc ttccattctg actcggacag
    14281 agatcatgaa cctgcgcgaa aggaaagccg ctcaaaggta acgcccctta tacaggtatg
    14341 ccgcggccat tgatcgtcaa cagattattt gaagcgctcc ggaaggtgat atcatgaaaa
    14401 atacgataac cgttttcttc tttataatgt gcttattact tgcaggttgc ggtgcggcgg
    14461 tgaatttaca ggggaaggtc gccggcataa gttccggcag gtttttctac caggatggac
    14521 atctggtcag cgattacaaa gccgatatcg atgcggtctg gcaggcctgt gaacaatccg
    14581 tgaccgatct gaaagcgaaa agtatccgaa aagaacgtaa aatttccacc ggcgtcatta
    14641 catccgtcat tcaggacgaa aaagtcacca tcaaagttga gtatgtcggc agagatatga
    14701 cctccgtttc agtctttgtc ggagcattcg gcaataccat ggcttcccgt ctgatccatg
    14761 acagaattat cggcaatctg gcgaagaact gattccgatg gcagataaaa tattgtcaat
    14821 ttcttttgac cagcagcaaa cctttcaaat aacgtccttc ggggtgtccg aaagcgaccg
    14881 gatgatccgg tccggcgtcc agccttgata aaagctgcaa ctcggcaccg gcatcccgcg
    14941 cggcacccag aacgatctta taaaaaagat cttcttcaat gaaattggaa caggaaaaag
    15001 tggccagcat cccgcctttg ctcagatgtt tgattgcctg taaattaatg tctttatacc
    15061 ctcttgccgc tttgggcaca tctcttttgg ttttggcaaa agcgggcgga tcaagaataa
    15121 tcatgttgta gttttccggc gtatgtctga aataatcaaa cacatcggtc tcgatcaccg
    15181 gataattctc cacagaaaac cagttgagat gcaggttttt tctggcggca acgcacgccg
    15241 ttttggatat atcaagagaa acaacctttt tggcgccgcc ttccgcacaa taaacggaaa
    15301 aagccgcggt ataggcaaaa caattcagca catgagcatc acggcccagg gaaccgattt
    15361 tttcccggtt cacccgctga tcgaggaaaa atccggtttt ctgaccggaa acaacatcaa
    15421 cttcgaattt cagaccattc tccataattt ccacggtaga attttcatct tcaccaaaag
    15481 cgatgccgct tctgttttcc aatccctcca gcttgcgtga atggctgtca ctttgttcat
    15541 aaattcttgt tggtttgagc tgggcgatca gagcatttaa aagatgattt ttctgtttct
    15601 ccattccggc gacggcgatc gaaaaaacca gagtcgtatt gtagacgtca acaatcaatc
    15661 cgggaaatcc gtccccttcg gcattaatca gacggtaggc atttgtctgc tcattgataa
    15721 gtctctgccg caatttatat gcgtcatgca aacgggactg ccagaaatac ggtgaaatat
    15781 tttcgtcgca tcttctagtc aaaacacgaa aagcgatatc cgtcgacgga ttaaaaaagc
    15841 ccagggccag cggatggcct gccgcatctc tggccagaac aatatcgccg gggccgggat
    15901 ttccttttac cgagcctatc gcgccggaaa atatccaggg atgtccgcgc agaatggcat
    15961 cttcccgagc tttctttaga atgatttccg gatagttgat cgtgatcatg gtggcataaa
    16021 atatattttc cgccttgaaa ttgcaagaca cattatatta taagcattca tcatttttat
    16081 ttttaatgcc aaaaaaacag ggagaaaaat catgtacgca gtgatcatgg ccggaggcag
    16141 aggcgtcaga ttctggcccc gcagcagaga aaagagaccc aaacaccttc tggacatcac
    16201 aggcgaaaaa accattattc aggaaaccgt taaccggata aagccgttgg ttcccccgac
    16261 aaatattctc gtggtgacgg gtaaaaaaca tgcccgggcg ttaatgaaac aactgccgga
    16321 aattcccccc cgaaacatcc tgatcgaacc ggtaggccga aataccgccg cctgcatcgg
    16381 actggccgcc ttgcatatcc gtaagcgatc gacggacgat acaatgatgg ttctgccttc
    16441 cgatcacgcc atagccgatt ccggaaagta tctgagcgtg atgaaagcgg cggccgttgt
    16501 ggcggcccag gagaaaggat tggtgaccat aggcattacc ccctccagtg ttcagacagg
    16561 gtttggttat atcgaagggg gaatcttatg caaaacaata aaaaaccaag acgttttccg
    16621 cgttaagtca atccgtgaaa aacccgattt tcgcacggcg gcaaaattcg tccggagcgg
    16681 caatttctac tggaacagcg ggatgtttgt ctggcagact tcggtcattc tggaagagat
    16741 caaacgctgg ctgccagagt tatatgcggg actgacactt atcgacacat cgctgggcac
    16801 cgccggcgag acggcaaccg tgtcccgcgt ttacaaaaaa ctgggctccg tctccatcga
    16861 ttacggcgtg atggaaaaag ccggtaacgt ttttatcctg ccgggaaatt tcggctggag
    16921 cgacgtcggc agttgggatg ctttatggga catctcccca aaagacaagc agaataatgt
    16981 catcctcagc gggagcagaa ccgttctcga agattcgaaa ggatcgctca tctataatcc
    17041 gcgaaaattg accgccttac tgggagtcag ggatctgatg atcgtcgaaa cgccggatgc
    17101 cctgctgatc tgtaaaaaag gacgttctca ggatgttaaa aagattgttg aaagactgga
    17161 agcaggccaa ctgaaaaaat acctttaacg gcgtttccag tataccggac ggcccttgag
    17221 ggggaatcct tttctttcga gtgctccggt aatgatatcc acagacgttc tctgatcgtc
    17281 aaaggtaatg gacacgacat tcccttcctc gaattgatag tgtttgacgc cttgaattcc
    17341 gttcaggata gcccctatcc tttgcttggc ccctcaggcg gcacatccag gcacggtcaa
    17401 ctgcatcacg acctcgcccg cgtcggccgc gctgacaaaa acgcccagaa gcatcagaac
    17461 ggcaaatata atgatttttt tcttcattcc ccaaaacacc ggttttattt gcgaacatca
    17521 taactccaat acccggcgga ttgcaatcat aattgcaatt gacagcaacg gttaaatttg
    17581 tgatacaaga aaaaaaatct attatgggga atgaatgtcc atcacagccc atgcgtcact
    17641 gattttagcg tcggcgtcac cgcgacggca ggaaatgctc cggtccgccg gtgtgcatgt
    17701 caaaatcatt ccggcccatg tcaatgaaaa atatctcacc ggggaaacgc ctcgccaaca
    17761 cgtgcgcagg ctttccagcc tgaaagcgat gaaggtggct gtcgagaacc ccgacgcctg
    17821 tgttctggga gccgatacca ttgttgtcat cgacggcacg gttctgggca agccccgcaa
    17881 taaaacacag gcgaaaacga tgctgaaaaa attaagcggc agacagcaca cggtctttac
    17941 aggatttacc gttgtccggc tgtcttttgg aattcgctcg accggggtgg ttcagtccgt
    18001 tgtacgcttc aaaaccattc atccggacga aatggagtgg tacatcgccg gtgacgaacc
    18061 ttacgacaaa gccggaggct atgcccttca aggccaaggc gcctgttttg tccgctccat
    18121 tcgcggttcc tacaccaatg tgatcggact gccgctctgc gaagttttgg aaacgctgaa
    18181 aaaactgcac gtcatcgatt tttgttagcc atatgggaac agacattgcc gccaatatcg
    18241 ccgctgtcaa acagagaatc gctgccgccg ccgaaagagg caaacggtct cctgattccg
    18301 tcaaactgct ggcggttacc aaaacggtcc ccgttccggt catcgaagaa gcgatccggg
    18361 caggcataac cgccctggga gaaaattacg ttcaggaagc cagagaaaaa atcgccgtca
    18421 tcggcagaag cattccgtgg cacatgatcg ggcatctgca gacgaacaaa gcgaaatatg
    18481 cggtgattct tttcgactac gttcattccg tcgaccgtct ggacctggct gcggaaatcg
    18541 ataaaagagc cgggcgggcg ggtcgtcaaa tcaacattct gattgaaatc aacgtcagcg
    18601 gtgaaaaaac aaaaaacggt attccggctg gaaaggcggt tgcgctgctt caggatcttt
    18661 ccgccatgca gcatatcgcg gtaaaaggat tgatgaccat ggcgccgtat tcggacgatc
    18721 ccgaaacatc gcgtccttat tttcgcgaac ttaaaaatct tcaacagaat atcatccggg
    18781 cgggaatcgc cggcatacac atggaagagc tttccatggg aatgacggat gattttgatg
    18841 tagccgttga agaaggcgca actctggtgc gcatcggcag agcaattttc ggagaaagga
    18901 attgagtaaa aatcccaggc gctgaaaaga cggccatcaa ttcacggtgc ggtggttggt
    18961 ctcgatcaac gttcgggcgg tttttttata gaccgtcaga caggcatcca cgacatcggg
    19021 atcatacgtg atcccccggt atttgacgat ttcctcgata gccgcatcca cgcccagagc
    19081 cggacggtac ggcctgtagg aagccatggc atcaatgaca tccgccacgc cgataatgcg
    19141 ggcttccagc gcgatctcgt ttcccctcaa ccccctcgga taccccgatc cgtccaatcg
    19201 ttcatgatgc tgatagatga cctccgccac cggataggga aattctatgt tcgccagcac
    19261 atcgttggcc actttgcaat gcgttttgat gatggccttt tccagattgt ttaatgcact
    19321 gggctttgtt aatatttccg acggaacgca tattttcccg atatcatgga gggtggcggc
    19381 aatatgaata aacgaaatct gctcctcggg taatttcatc tcccgggcaa tcttgcaggc
    19441 aattttggcc acctggtctt cgtggccggc cgtataagga tcccgcgtct ctataatttt
    19501 ggacagcgtt ctgacgatgc cgtccataat gatctgcgat ttttgataac tttccttgag
    19561 tttttccgtt tgaatcaaaa ggtcattggt tttctccaga gccttttgct ccagagcctt
    19621 gtttaactcc gtcagctctt tatttttctc tcttatcaat tccaccagac gcttattttc
    19681 cgctaaaatc tcaactttgg aaagcgattg tttaatatgc agcagaattt cttcttcatg
    19741 ccagggcttg gtgaagtaaa gatgtacgcc gcctttattg atggcttcaa taatggcgtc
    19801 ggagtccgca tacccggtca gaagaatacg gatggcatcc ggaaaaattt cttttgcctt
    19861 cgagaaaaac tgaacaccgc tcatttcagg cattctttga tcggaaataa taatagaata
    19921 gggttttgtg gccgaacgca gtttgttcaa tccctccaaa ccgctgtttg cgacatgcac
    19981 gtcgtaattt tcgtcggcca gaatgcgctt aaaagcattg agaattgaat cttcgtcatc
    20041 aacaatcaag atgcaatgct catatccgga atcgttagac aggttaatat cctttcctga
    20101 tgacaccgct gaagaatttt ataaaataaa aaaccattct catgaggatg tcaagatatt
    20161 tttactttca gacaacgaat gacctgagga agcactcaat gtttctgcaa gcagggaaaa
    20221 atgacaggat caaaaaggac atattttcag gccgtgatca catctctcaa aaatttgccc
    20281 gtcacggatt tttcatctcg cgctactttc tcgaccgttc ccgacgaaat aattctgccg
    20341 ccgccggagc cgccttccgg cccgagatcg atcacgtaat ccgcacactt gataacgtcc
    20401 aggttatgct cgatcacaac catggtattg cccatattca ccaggcgcat caaaacatcc
    20461 agcagtttct ggatatcggc aaaatgcaga ccaatggtcg gttcgtccag gatatacagg
    20521 gtattgctgt tcactttctt gctcagttcc cgagccaatt taatccgctg cgcctctcct
    20581 ccggatagtg tcgtcgccga ctgtccgaga tgaatatatc ccagcccgac atcgccgagc
    20641 aactgcagat gcgaccggat ggaaggaata ttggaaaaaa aggtcaacgc ctgattgacc
    20701 gtcatgtcca gaacatcggc tatgctttta tccttgtatt taatatcaag ggtgtcgcga
    20761 ttaaatcttt tcccatgaca ggcttcgcag gtcacgtaaa catccggcag aaaatgcatc
    20821 tcgattttga ttaaaccgtt tccttcgcac gcctcgcagc gtccgccctt cacgttaaag
    20881 ctgaagcgcc ccggtttgta tccgcgcatt ctggactccg gaagattggt gaacagatcg
    20941 cggataaacg aaaagacgcc cgtgtaggtt gccggattgg agcgcggcgt gcggccaatg
    21001 gggtgttgat tgatcataat gacgcgttca atcccgccaa gatccacaac ccttttgatt
    21061 ttccccatgg atccgttgta ctgattcaaa cggcgcgcca gcactttgta gagtgtttca
    21121 atcaccatgg tactcttccc ggatcccgaa acaccggtta ccgccgtaaa cacacccacc
    21181 ggtattttga tatcgatatt tttcaggttg ttttcgctgg cgccttcaag aataatgtgc
    21241 cttccggtaa gcggacgttt ttctgaggga aggttgatcg attctttttt cgacaaatac
    21301 ctgccggtca gagacgtttc actgttcaga atctgtaccg gcgtgccctg aaaaatgatt
    21361 tctcccccct ccgttccggc ccccggtccg atatctacga tatggtcgca ggccatgatc
    21421 atgtcggcat cgtgctcgac aaccagcacc gtgttgccca tctcgcgcag tttcttaagc
    21481 gtatcgatca aacgcagatt atctttctga tgcagcccta ccgtaggttc atccagaaca
    21541 tacaaaacgc ctgtcagccc ggaaccgatc tgcgtggcca gccgaatgcg ctggctctcc
    21601 ccgccggata atgttgaggt ggctcgctcc aggttcaaat aatccattcc gacattgagc
    21661 agaaactgca accggctttt gatttcccgg atgattcttt cggtaatatt gagttcctgt
    21721 ggtgagagtt cgatccgttc gaaaaagctg tggcaatcgc ggatggacat tttgcacaat
    21781 tcatagatat tttttcccat caccgtaacg gccagaatct cttctttcaa acgcgccccg
    21841 tggcaggtct cgcaggcgct caaatcgata taacgcatca gatcattgcg aatcgccgtt
    21901 gacgcggttt cccgccaccg ccgctccaat tgccggattg ccccctcaaa cggccggtga
    21961 acaaaatatc ttttattgcc gcgatcatga tagaatttga ttgtttcgct gcccgacccg
    22021 taaagaagaa ctttccgaat tttttccggc agcttattga acggcgtgtt cacatcaaaa
    22081 ccgtaatggc tgctgagcgc gtccagaatg ctgtaatagt aaagcgagtg acgaccggcc
    22141 cacggcgcta ttgcgccgtc ccgcagagac aatccggcat cgggaacaat cagatattcc
    22201 gtaaaacgca tggaagaacc cagaccgttg caggcgggac aggctccgta gggattatta
    22261 aatgaaaaca tgcgcggtgt cagagcgggc aagctgatcc cgcaatcggg gcaggcatat
    22321 ttttcgctga aaagaaattc ttcgccgccg gcggtcacga tccgcaccag cccctcggca
    22381 tgcttcgacg cgatctccag agaatcccgc aatctttttc tgataccgtt tttcagcacc
    22441 aaccggtcaa tcacaaggtc aatatcgtgg cgtttattct tttccaggct gatgtcttcc
    22501 gccaggtccc tgttttctcc gtcgatccgg acacgggcaa acccctcctt ctggagtttt
    22561 ttcaattcct tctgaaattc gccttttttc cctctcacca tcggcgccag aacactgaac
    22621 ggcgtgtttt ccggcaaggc aagaacattt tccaaaatgc cgtcgatggt ctgtgatttt
    22681 atttcccggc cgcaccggta acaatggcag acgccaatgg cagcataaag caagcggaaa
    22741 taatcgtaaa tttccgtcac cgtccccacc gtggaacggg gattatggct ggccgtacgc
    22801 tgctcgatgg caatggcggg agacaagccg tcgatggatt ccacattcgg tttgtccatc
    22861 tgtccgataa actgacgggc ataagcggaa agtgactcca cataccgcct ctgcccttca
    22921 gcatagatgg tgtcgaaagc cagcgatgat ttgcctgaac cgctcacacc ggtaatgatg
    22981 acaaattgat cccggggaat atccaagctt atatttttta aattatgttg agcggcgccc
    23041 ctgattttga tcgcgtccat gttattcttt gctttaattc ttgatctcta tatgcgattt
    23101 tgcgcgaatg gcactgatcc attcctcaaa ttttttatcc agtttttcat cttcaatttt
    23161 tgcttttatc tcctcccgga ccacggcaac cggttttaat ccggcgccgc gtttatccac
    23221 gacctttatg atatgaaaac ccgcatcgga ttcaatcacg ttactgacct gatccacggc
    23281 aaggctgaac gcaaccgcct caacttccgg gatgatggtc cccttctcaa taaacccgac
    23341 atccccgccc tgagcggccg ccggcccctg agaatacgtt gaaaccagta ggtcaaaaga
    23401 ttctcccgcc atgatacgtt ccctcaggtg caaagcttct ttccggattt ttccttttgt
    23461 ctccgcgttg gcaccagaag gcagcagcaa taaaagctgt ttcattctca cgctttcttt
    23521 accctcgtaa tcattgcggt gctgattgta ataggcgcct atttcctcat cggtaacgat
    23581 aatcttatct ttaacttccc gtcttaataa tcgcatacgg atcatttgtg accgtatttc
    23641 ttttttgacc tcctcaatgg agctgccgtc cttggcaagg tttttcaaaa aatcctgcat
    23701 actgatattc tgtttggtca gcatgtcctg aagaacaccc ataacgtctt cttcccggac
    23761 ggaagcactg atccccgttt tcttcgcctc ctgctcaatc aggatattgt cgatcagccg
    23821 gttcagaaat tcctccctcg catgaagaac aaattggtcc ttgtcgccac cctggtaggt
    23881 attctcaata tttttccgat acggttcgaa ggctgcattc aactcaagcc gggtgatcac
    23941 ctcatcattg accacggcaa tgattttatc cacaacttcg gcgtgcaata acggacacaa
    24001 aagcatgatt cccagtaatg caaaaacata ttttccgtaa aatttcacca cagacctccc
    24061 ttgatcgtgc tacctgtatg atgtattgat tttaatatcg gctttttttt ggacaacggc
    24121 ttttttcttt aaggcttcca gccattccgc aaaagccgtt ttctccttca gcaggcgaat
    24181 atccgcaatc acttcttctc tggcctccgg caagattctt tctttggcct gccggacttc
    24241 catcaccttg ataatatgaa atccataaga actctgtacg atcggactga tcgtctggac
    24301 cggtatttta aaaatcgttt catccagcgg ttccggcatg acccgtttcg tgatgaatcc
    24361 caggtcacca ccacgcgagg cttccggacc gatagacatc tccgaagcca cccgggcaaa
    24421 ttcttccttg gatttcaatc gttgcatcgc ctgctcggcg gtttgcatat cccgaaccac
    24481 aatctgcgac actctggcag acaaatccct tttgtagatg tgacggtgct ttttgaaata
    24541 atcttctatt tccgtttcgg aaactataat tttagaatga acatccgccg caataagttt
    24601 ctgcagaaga atttcctttc gaaaatcctc tttccatttc tcatagttga tattttgtct
    24661 ggcaaaaaga ccggtgaatt cgtttccgta atctttttcg atttctttaa tttttgcctc
    24721 cagttcggca tcgctcaccg acaggttcaa ttcccgggca cgcaagagca ttattttttc
    24781 cccgatcatg gcatccagga cttcgtcctc gaattttttc acatacgcgg gctgtctaaa
    24841 gaaatcgccg gaaagcatcc tggccttctc attcaaaagg gactggtatt catcaagata
    24901 aattttggca ccgttgaccg tggcgacatg gggacgcgaa aagctttcca ttttatcgca
    24961 ggcgatcatt aaccccggca agagcagaca caaaagaccg cagcgaaccg gagaaacctt
    25021 tttggatttt cccgccaaag aataaaattt agaaaaccat ctggttggaa tcatcatctt
    25081 tctgtatatc ctgcttaccg ctattgggca agcatcttta acaacatatc cgcctgaacc
    25141 agaatgtcca tatccgcagc agccggcgcc ggcacaaaca gtttatggtc cggggtaaaa
    25201 cgcaattctt tgattttctt gcggtaaaga gcgattattt tccccggatc gaccggagaa
    25261 ttctcccgga agaagatgta taggtattta ccgtcatagc ccatcttctt ccctttcagc
    25321 ggcttcagaa catttcggat ggagatcacc cgcaataaat tatccgcaac acggggcatc
    25381 tcgccgtaac aatccagcaa ctcattttca atcgcgcgga tgtcttcatc gtttgccgcc
    25441 agggatattc ttttatacaa aatcagacgc tgatggacat ccggcacata atcctcgggg
    25501 atgaatgcgg aaatcccaag ctgaatttcc ggcatccagt cttcttcggt tttctccccc
    25561 ttgacttcgc gaatggtttt ttcgattaat tcagtataaa gctcgtaacc aacggcggaa
    25621 atatgtcccg actgggacat ccccagtacg cttccgccgc cccggatttc cagatcgttg
    25681 tatgcaatcc ggaaaccgga gcccggatcc gaaaattcct taatgacctg cagccgtttc
    25741 atcgctgcgc gggaaagtat cgctccctga ggcagcaaca gataggcaaa cgcctcccgg
    25801 ctggatcgtc ccacgcggcc gcgtatctga tagagctgcg ccaggccgaa cttttcagcg
    25861 cggttgataa taatcgtatt ggccgtcgaa atatcgagac cggaaccgat gatcgttgta
    25921 caaaccagca cattgttctc ttgccggatg aacccggcca tggttttttc aatttccgcc
    25981 ggcttcatct gcccgtgaac aactccgact ttggcgtgcg gcacgagccg ctgcaccagg
    26041 cgggcaatcg cataaatcga acgcacccgg tcgtggacaa aaaacacctg cccctgacgc
    26101 gccagttctt tttcaaccgc cgttttaatc gcatcctcat cgaactccat gacataggtt
    26161 ttgatggaaa cccggtcctt gggcggcgtg ttgatgacgg acaaatcccg gatgccgacc
    26221 agcgacagat gcagcgtcct gggaatcggc gtcgccgaga gcgtcaacac atcgaccagc
    26281 gtgcgcattt ttttcaactt ttccttatgc gccacgccga aacgctgctc ctcatcgata
    26341 atgaccagcc cgagatcttg aaacgcaacg tccttttgca aaagacgatg cgtcccgacg
    26401 acaatatcga ttgtctgatt tttaagctct tcgacaattt ttttctgttc ggcgggtttt
    26461 ttaaaacgat tcagcagtcc cacccgcacc ggaaaatctt caaaacggcg ggtaaacgtc
    26521 tgatagtgct gttccgcaag aatcgtcgtc ggggtcagaa cagcaacttg tttaccgtcc
    26581 atcaccgcgc gaaacgccga gcggatcgcc acttcggttt tgccgaatcc ggcatcgccg
    26641 cagatcaggc ggtccatcgg cttgacgtca tccatatcct gatgaatgtc ttcgatggct
    26701 ttcacctgat ccggcgtctc ttcaaattca aaggtcgagc aaaactcttc gtagattctg
    26761 tccggcgggg caaacgattt tcgttccagc gtctcgcggg cggcataaat ggacaccagt
    26821 tcttcggcat agtcgcggac cgattttttc accctttctt tgacagcctc ccaggatgtt
    26881 ccgcccatcc ggtcgatctt cggcacatag ccgtcgggac cgacatagcg ttgaatcttc
    26941 tccagagcat taacgggaat gtataattta tcgccctccg aatattcaat gaccagaaaa
    27001 tcgttttcaa tctttctcac ggcgattttt ttcaaaccgc ggtaaatgcc gatgccgaaa
    27061 tccgtatgca cgacaaaatc gccttcttgc agatcgccga acgatttgat aaaatacccc
    27121 gtgcgtactg gccgcgagcg cagacggcgg gcttttttga cgaagatttc ttcttcgctt
    27181 aagacggcca gctttatcgc cggcatgaca aagcctgatg aaatgtctcc cacgaataaa
    27241 atcaaccgtg catcgccttt ccaaccttcc aggctgtcca gaaccggttc agcgccggac
    27301 aatgtctccg ccggcagatc gtaagacgaa agcaacccct tcatccgttg aacatcttcc
    27361 ggcaacggac agaaaagaaa aaccagcatg tccccggcaa accatgattt tattttttct
    27421 acggtcaacc gtagcgttgc atcctctcgg atttcacctg ccagcggctc ccctgtgcag
    27481 gtgaaccggt cgctttcaaa aactaccggc acatgatatc tttcagattc gatttcgagt
    27541 ccagccagcg caacctgtag gaagccatcc aggccggccg ttacagcctc cggcgggaaa
    27601 taggtatttt ccttttccag atagaatttt ccaccgctct ttgccttgaa cagcaattta
    27661 tccatagcca acccagcgtt gaacatcgcg tgcccaacag ccagcggatc atccagaacg
    27721 accagtgtat ttcttgaaag atattccaaa aggccggaca aggtattatc ggaaaggccg
    27781 cttccggtat cgtacgattc ataaaacagc ggcaagaata tcggattgaa ggcattggtc
    27841 aacccctcct gaacggcggc aaccagacgg ctgcgaatgt cgcgggaaaa ggaaagttcg
    27901 tcggcacggc gccggatgtt gcgcactgcc agctccagcg tcgaatgatc catgacggcc
    27961 tctccggcag gagataatac aaaagcgggc aacggtccga tagaacgctg ggaagatgtg
    28021 ttgaatgtcc tgatggactc tatttcatcg cccagcatct ccaggcgcag cggatttttt
    28081 tcggtgggag gaaaaatgtc aatgatgttt ccgcggatgc tgaattcccc tttttcctga
    28141 accagacttt ctcgtttata gcattgcgcg acaagctgcg ctgcaaattc atcccggttg
    28201 atggtgtcgc cgggagcaat aattcgcaga tgcttgctga actcggccac cggcatcact
    28261 ttctgcatca acgcatttgc agaggcaacg acaataattt tgtgttgaat ctgcaagttg
    28321 gccaacacct ccagacggga caattcctcg tcacgctgaa gagcaaacat gtcaatggtc
    28381 aggaaatcca gcgccggata atgaaacacg tgctcttcac ccagaaacag cgacaaatcg
    28441 cgagcaaagg ccaccgcttc cttctcctcg gggcagacca ccagtacagg cctctgtcgt
    28501 tttttataca ataaggagat cagaaaagaa cgggccgcgc cggcaaggcc ctgtacggcg
    28561 atgcgcatca tcccgtcatc gatctgtttc agcagaagac gaaacgactc catatcttcc
    28621 gggtttttcc ttgtgatcat tttccgcacg gcaatcaatc ccccaaaaaa tcaaacaaca
    28681 ccaaaaccca ttccgcatcc aaaaagttct tttgatccgg aacgggtatg tttgcacgtg
    28741 ttctattctt ctttcccgga taattcaatt gaaaacttgt tttccgaaaa ttgccattaa
    28801 aaatctatct ttatattgac aagcgcggca tctttgataa actggaacgc atcaattccg
    28861 cggagattga atatgattcc tgtagaaaaa gccttaaaaa caattctggt taatttccag
    28921 ccgctgggcc tggaaacagt ccatcttctg aaagcgaggg gacgcgtcat cggcgaaaat
    28981 attatgtccc cgcgtaatat cccctctgcc gataattccg ccatggacgg ctatgccgtg
    29041 cggtgcgccg ataccagggg cgcgggcaag acaactcctg taaaattgaa aattatcgaa
    29101 gacattcccg cagggaaatt tccccgaaag aacatcggca aaaacgaaac ggcccgcatc
    29161 atgaccggcg ccgtgattcc cccgggggcg gacgcggtta tccgtcagga agacacccgc
    29221 aaagacggca gaaccgtcat cattttcgct tcagccgcga ggggggaaaa catccgcttc
    29281 gccggtgaag atgtccgtaa aggcgaatcg gtcatcccaa aaaacagctt gctgcgtccc
    29341 gcgcatatcg ggatgcttgc cgcgctggga aaatcctcgc tcaaggttta tcaaaaaccg
    29401 cgcgtggcaa tcatgtcaac aggcgatgaa ctggtcagca tcaggaccaa cccgccgccc
    29461 ggcaaaatcg ttaacagcaa cagttattct ctggcagcgc aagtagcgga gtgcggcgcg
    29521 atcccgttga tcctcgccat tgccagagat aaaaaatccg atttggaaaa aaaattcaaa
    29581 aaagcgctga acgccgatgt cattatctca tccggcggcg tgtccgtggg cgatttcgat
    29641 ttcgtaaaaa atgtcatggg agaaatcggc aatgccatgc atttctggca ggtcgccatg
    29701 agaccgggga aacccctggc gttcggtgcc atagaaggcg ttccgctgtt cggcctgccc
    29761 ggcaatcccg tatcggcaat ggtttccttt gagcaatttg tgcgcccttc gcttttgaaa
    29821 atgcagggcc ataaaaaaat cttccgtcag accgtcacgg caatctgcca cgaagaattt
    29881 gaaaaagctg ccggctttag acatttcatc cgtgccgtcg tccggaagga gaaagacaaa
    29941 tatattgccg tgacaaccgg cgatcagggc tccggcattt tgaaatcgat ggtcacggcc
    30001 aacggcctca ttgtcatggg agaaaacgaa acccgaatca aaaaaaattc ccaggtcaca
    30061 gttcagttgc ttgatgattc gttatcctgg tcggatgctc tgcaattatg aaaatacgat
    30121 ccgagatgaa aattctgctg ataaactttt gatgattgac acttttcttc tttccggata
    30181 gattcttccg cggaagtgcc aaggtcactg gtgggcctcc cggacttcaa atccggtgtg
    30241 gggcgctaaa accgtcccag gtgggttcga ttcccatgca cttccgccaa tattatcatc
    30301 atcgaaattt gtgacaacgt tatcaacgct gcattcagac gtaaacgatt taaacaatct
    30361 gacctcaacg gcgaggttgt ttttgcaacc gatgaggcat tccggacaac acaggaaaag
    30421 gaaaagttta caatggattt gatagaagcc attcagcatc gcagaagcat ccgggcgttt
    30481 acggatcagc cggtgcccaa agaaaccatc atggaaattc tcaatatcgc ctgccgtgcg
    30541 ccgtcggcga tgaatacgca gccctgggaa ttcattgtcg tcagcggcga gcccctcaac
    30601 aaactgcgtg ccgccatcgt tgacaagctg acgtccggtg cgccgattac accggatcat
    30661 caggtggtgg gctggcctga taaaagcgtt tataaggacc gccagatcac gctggccaag
    30721 caactcttca aagccatgga cattgcccgt gaagacaagg aaaaacgcgc ctggtggatg
    30781 gaacggggat ttcgtttttt tgatgctccg gcggccatca tcattgtcag cgacaaaata
    30841 ttaggcgatc atgcttccct gatggacctc ggcgcggtca tgcaaaacat ctgtctgacg
    30901 gcgctgcact tcggagtcgg cacctgtatt gaagatcagg gggtcatgta tcctgaagtg
    30961 gtccgccact gcaccggcat ctcttcaaac aagcgcctga tgattgccat tgctgtcggc
    31021 tatccggacg aaacattccc tgccaatcac gtccgctcgg agcgcgaacc ggcggaaaat
    31081 ctcacggcct gggtggaata aatgatccgc gttgaaatgc aaatcggaag aagacctttt
    31141 tatgtataag atgatgatta cacttctatg tatctttact ttgctcggat ccccggcaat
    31201 gagcgaaacg acgtcggact ggttcaacaa ggctcaagct ttatcggatg gtgaaaaatt
    31261 cagtgatccg caaaaagcca tcgagtattt gaataacgcc atcagacttc agccgaatga
    31321 tgccgaggca tactataaca ggggaattgc atacgacaac cttggccggc atcagcaggc
    31381 catcaaagat tacagtcaga ctgtccgcct caaacctgat tacgccgagg cttttcataa
    31441 cagggggacc ttatacaaca cgaccggtca atatcagctg gctattgcgg atttcaacga
    31501 atccatccgc atcaatcccg gagaggccga caattacatc ggccttggtt ttgcttatga
    31561 tcaacttggt caacatcagc aggccattac ggaatacagc aaagccatag cgctgaaacc
    31621 cgacaacgcc agggcctaca acaaccgggg ggctgattat ctgtcactgg gaaaccatca
    31681 atacggttgt ctcgacgcaa aaaaggcctg cgaactggga aattgcaaat tacataaaat
    31741 agccgaaaag caaggggttt gccgttaaca tgctcaaaaa catccggtcc cagaccgttg
    31801 cgcttttttt ataaatcatt tcttttccgc aatagaatta acaatttttt attcattgaa
    31861 ttagcgtgtt cagtctcgcc attgttcttt ttcaaaatta atcgatatta agcaatatta
    31921 atgaatatta agcaatatta atgaatatta aaaaaaattc ttgacattta tttaaaatta
    31981 gttccttatg agaagcaagt gaggtgtcgc tttaatctat ggcagataat atgaagaaaa
    32041 atcacgtaaa aaaatttcgt gagacaattc ctttaagcat atccgaactg gctcgccggg
    32101 ccggattaac acctcaaaca atttccaaaa tggaaaaagg attgtccacc agaaaaaatt
    32161 ctgaactcaa ggtcgctaaa gcactgcaaa ggccacatga agaagtattc ccttgatgaa
    32221 ggcgaataat atattcaatc atttccgtac cggagtagtt ttgaatttcg atgaagattg
    32281 tcggcagatt gctaattata tttttgctgt taatgtcgct tttgatcaca ttcggcaaca
    32341 ggggtctcgt tgacaattac ttgatgagca aaaggctggc ggaattaaaa tcgcaaaaca
    32401 gcgccataac agcagaaaac aacgcattaa aaaagaaaat tattttattg cgcaacgaca
    32461 ttaattacat agaaatgata gcccgtaacg aattgggaat ggttaaaaaa ggagatattg
    32521 tttaccggtg ggcaaagtaa agatgacttc ccgataacca atttatggtg ctttgatggt
    32581 ggatttgaaa gaaatatttt ccggaaaaaa gagtctcgtc ggtttggata ttggttcaag
    32641 ctccgtaaaa cttgcggaaa tcatcagcaa cgccaaagga tactatctga accgtttcca
    32701 tgaagttccc ctgcccaaag gcatcattat tgacggcatt ctcgccgatg cacccgtgct
    32761 atcctcaaaa atcaaggagc ttttcaagat tgcccattgc ggccgtaaag gcgttgtcac
    32821 gtctctggcg ggaaattccg tcatcatcaa gaaagtgacg ctggagcaga tgaacgaaac
    32881 ggaattgcgt gaactgatcc acgatgaagc cgggaaatat ctgccttttg ataacatgga
    32941 cgatgtcaat tatgattttc aaattctcgg cgaaaacaat tacaatccga atcagatgga
    33001 tattgtcatt gttgcagcga aaaaagaaat tgtaagcagt tatctggaag cagtaacagc
    33061 agccggccag aatgttgaaa tcgttgacgt ggctccgttt gtcctggaaa cattatatga
    33121 agtcaattat gaatttgaca acgaggaaat cgttgttctg atcaatatag gagcaagcac
    33181 caccggcatt aatgtggtta aagggggaat gtcggtgttc acaagagatt tcgcgctggc
    33241 aggcaattcc atcactgaaa gtatccagga aaaatatcag atcccctttg aagaagcgga
    33301 aaagcttaaa atcgaaggat ttcccgaggg aagcgaaaac gacaacctgg atctgaaaaa
    33361 taacatcctg gaatttgccc agccgatttg catggaaatt gaaaggtcca ttgactattt
    33421 ccgttcaacg tttggcggcg aggatatcaa gcaggtgttg ctcgccgggg gctcgtccag
    33481 aattcccaac atcaccaaac atttatcgga attgctcaat gttaaaaccg aaatcatgga
    33541 tcctttttta aagattaatt acaacaaacg aaaaattgat gtcaaaaatt tagacagcat
    33601 caagccgatt gcagcaaccg ctatcggcct gggcttgagg aaaataggcg acaaatgatt
    33661 aagattaatc ttttacccta tcgtgagaag gccaagaaag atacgttcct gagacaaata
    33721 tttattgtcg ccggatccgt cgtgttgttt attcttttgc tcgcctgggt aacaatctgg
    33781 ataaaatctt cgatcagtaa cctggaaacg cagatcaaag attccgaagc tttaataacc
    33841 gatttgaata agaaaattgg cgatctggaa aagttcaaaa aagataaaaa agaactggag
    33901 cagaaaatag gcgtcattgc aactctggaa gaaaaccgcc tgacgcctct taaaacactg
    33961 gacaatttag cgatgcttgt tccgccgaaa gacatctggc ttgtccggct gactcagaaa
    34021 ggaaattctc tcaatattga ggggatgggc cgggataaca ttgctgtcgc aaatttcatg
    34081 aaaagcattg agcagttcga accggtacaa tccgttgatc tggtctcttc caaaaagacg
    34141 gaaatctccg gcaatgctgt ccaacagttt aatttcgcct gtattctgaa aaaagggttt
    34201 tgaaatatgg ccatcgacat caataccatc aagaaaattc cccctaagac aaaagtattg
    34261 attgtgattg tcggattttt attaatcggt tatttttact ggttttattt tctgtcttcc
    34321 gccatggaaa aaagaaccac tttaaatacg caacttacgg agatgcagaa taaaatcaat
    34381 gagcaaaaaa agatcgctat gcagcttaac aaatacaagg ccgatgttga agcgttgcag
    34441 gcaagttata agatagcgtt acaaaaactt cccgaccagc gggagatacc ttcacttttc
    34501 cactccgtcg cgtcagcggg cagggacgcc ggagtggaat ttgtattatt tgaaccgaaa
    34561 gcatccgtgc ccaaatccct ggaatcccag ggacctgcgg cttcgaaaac cgcagccttg
    34621 ctgaaacctt ctgatcaacg cgatcaaaaa ccggcggatt cttctgaaaa gaaagatggc
    34681 gcggctccgt ctacggaacc tttctacgag gaaatacctg tcggaatatc ggttttagga
    34741 agctaccaga atatccttta ttttttcgac aaagtttcca aactgcccag aatcgttaat
    34801 gtatccgaca ttacaatcgg agaccgaaaa gaaatcaaag acaaaggcca tcttgttacc
    34861 ggatcctgta cgataaaaac atacatgttt attgataaaa aagaaaagac aagtgaaaaa
    34921 acacaatgaa aaaaatattt ttgattttca ttaattgtag cctgatcctg ttgaccactg
    34981 ctttttttgc ttttgcggca gaggcccaaa atacggcctc gcctgctcca aagtctgtag
    35041 cccccgttaa aggcaaggtc caaccggcac cggccggaac ggttgttcca ggtccggccc
    35101 ggacggctct ttcgcccgca gccgcaaaaa cgcctgttcc ggctccggca aaacaggctc
    35161 cggccgtttc cccggctcaa caaacaacgg tttcttccgc ggcaaatgcg actgcaacgc
    35221 caactgtcca aacccctatg ccggcaccaa ccgtgccgaa tcaggcggac acatacagtt
    35281 acaatcccgc gggtaagccg gatccgttcc gaccttttat tgtcattgaa accgtcaaaa
    35341 aaacgaagcc ggaagagaaa aaaatcaaac cgtccatttt ccctttgcaa agagcggaag
    35401 ccagaagtta taaagtcgtc ggcattgtcg gcaatgagga tcatcgggta gcgatcgctg
    35461 aagacgcttc taaaaagttt tatcctctgc tggtcggaac aagaatcgga ttgcataacg
    35521 gcaaagtcgt tgacattctg cctgacaggg tcattgtaga agaatatgaa aataataaag
    35581 gaaacaaagt aattttaaaa ctgcacaaaa attaaaatga ggtaaagtca tgaaaaaata
    35641 tttccctaaa atattcgcgg cttttgtaat tatcacatgg atcttcacat gtatcatggg
    35701 aaattctttt gcagctttca atgcttccga agaaaacact gccggcatca ccatgttgaa
    35761 aagtatcatg ctggccaagt tgcccggaaa agaaaggatt catctggttg tttccaagca
    35821 accgctcatt gatgttaaga gcaaagacaa cggaagctac ctgatcagac ttgaagatac
    35881 cgatatcccg gaaagtctct gccgcccgct gggagaaggg gaactcaaca atattatcag
    35941 tatcgttcct tcgcggcagc agctccaggg gaaaaactgg gtttatctga cgatttatcc
    36001 gcatgaggcc gttccctatt caataagaca ggaaggggaa aatgttttaa ttgattttaa
    36061 tgtttccggc attgaggaaa gaaaggccag aacgatatca aaagcctctg cttctaaaaa
    36121 gcccctggcc aaagtcagcg cagaaccgga aaaaaccgac gattttaaaa aagaagacct
    36181 ggacgtgtcg gtaaaaagat ctccggcgaa aaaatatatg gatagaatcg tctctcttga
    36241 ttttcaggat gcggacatca aaagcgtttt acgcctgatg gctgaatacg gcaatgtaag
    36301 catcgtgacc agcgaagaag tcaaaggcaa cattacgctt tccatgaaaa atgttccctg
    36361 gacaaaagcc ctggaaacca tccttgaagt taacggtctg acaaaaaaac aggaagacaa
    36421 cgttattatc gtaaccagca tggaacgcag caagcgagaa gaaaccgcca gagaagaggt
    36481 ccgggccaga gaacagaaaa tgatggccga aaaaggatta ttgaagcagg tcttgattga
    36541 agcaaaaatt gtcgaggcaa ccgaagaatt cgtcaggaac ctcggtgtcc aatggggatt
    36601 tggtacggta cagagacaac acatcggcag cagtcagtat ggattgggcg catccggagg
    36661 cagcaatgtt gccaccggaa accagcaggc gcttacatat ccttctcaaa tcggcatcag
    36721 ggatgccgat gggaatccgt tagcgatggc cgcagtcaat ttgcccacat ccctggcttc
    36781 gccggcaatc ggtctgatct ttgggggcgc cagtggtttt cttgaaacgc aacttgccgc
    36841 actggaaacg aattccaccg gaaaaatcat ttcggcgccc aaggtggtga ccatggaagg
    36901 tatcaaagcc attattaaac agggcgatga agtgccttat atcactccgg catcgggaac
    36961 atcgccggca actgtttcct ttaaggaagc attattaaaa ctggaagtga cgccgaagtt
    37021 aaccgacgaa gggaaaatct ccatggatat caaggccagc aatgatactc cggactggtc
    37081 gaaagcagcc ctgaatcccc agggcaatcc tccaattgtc aaaagcgagg tggagtccaa
    37141 ggtggttatt aacgatggag acaccgtcgt cgtcggcggc attttgagat caacggaaag
    37201 caaagccgta agtggctggc cgtggctgca aaaaattccc gttctcggct ggttatttaa
    37261 aacggacagc acaaaaagag ataaacggca attgctgatt tttgtgactc caaaaatatt
    37321 gaataacgac tttacagcag aaggaacctg aaaaatccca actgccggcc atcagattat
    37381 cggcttttat ttgactctga tctgcaatcc agtatcggat ttgtaacaga ctggtttata
    37441 ttaaaatgaa actaaccgcg aacaaagctg tattaatatt tgtgttctca gcaatcctgc
    37501 tgtgtttttc ctgtacatat acaccctggc acagagaaca gtccgagatc ttcttgaaca
    37561 aaggcatttc tttcctgcag atcggacaat tcaacagcgc tctgaaagaa ctcctggaag
    37621 ctgaaaaata ctattccaga aatcccaagg tgcattatta tctgggaatt gcctatcacg
    37681 gcaaaggcat gagagaacag gccatcgagg aatttgaaaa ggcggtctct ttggacaagc
    37741 attattccga agctcacaac tacctgggca cgctatattc ggaaatacag ctctatgaca
    37801 aagccatcag agaattcgaa aaagccctcg gcaatcattt gtatgatacg ccgtccatgg
    37861 ctctgtataa tatggcctgg gcctattatt caaaaaaaga ttacaaaacc gctctggata
    37921 aatatcagga tgtgctccga acagatccca tgacgggatt gcgtccgcag gtagaaaaaa
    37981 atatcggcct tatccattat gaacaaaaca atgacatgga agccatcagg catttaaaaa
    38041 aatcgaccga gctggatcca tcgcttgtgg atgcctactt tattttaggc cagagctacc
    38101 tgagaaacaa agacagcaaa aacgcacgcc gggcattcga aaatgtcgtc aagttgtccc
    38161 ccgattcttc tttcggattg aaagccagaa attacctgca atcattacag taatctgtgt
    38221 aaaacatcct gtcttttcca ttcttgacac tccccatgtg ctgtgttacg taacgccaaa
    38281 ttgcacattg atttaattat ttgattaaac atggaaaata gcagatttga caaaaaacaa
    38341 aatctccttt tacgcaaagc agaagcattt ttgcgggaag gaaatttcgg ggaagcgctc
    38401 cttatcgccg aaaatcactt gctggactgt cccgcagatg ccgatgccct gggcatctgc
    38461 tgcgaagcgc ttattaacat gggaagactg gaagacatgc gtaacatact tcttcaaacg
    38521 gaagaatgta tttccgcgtt aaatcttgtt tgtgagcgtt ccggcgacgc ctgccgtgaa
    38581 aaaggttttc atcaggaagc tgccgcctgc tacgaaaggt tcatctcttt gcggccggaa
    38641 acggaaaaag cgtctgaaat cattgaaaaa atgaccttgt tggatcagga agaccatccc
    38701 accgtcccgg aaaagggtaa aagaaatatt cctgcacagg atttttttac agttaccctg
    38761 gctcagttat acattgatca gggacatctt cagaacgcaa aaaccatttt agacgaaatt
    38821 ctcaaaaaag accccgataa ccggcaggcc ctgtccatgc tcaacaacct catatccgcc
    38881 ccggctgcat cgggaaccgt aaacggcaac acaaagaaaa acgacgagct gatcaagatt
    38941 ttatcttcct ggctgaaaaa tattgaaaga ttgaaaatga atgccgctta ataataaaaa
    39001 aatgccgcaa agactttccg gaattctgga aaaaatgtcc gtttgtaaaa tcgacggtct
    39061 gatcatttgc aatatgagca ctgtccgcta tctgtgcggt ttcaccggct cggaagggct
    39121 tctcttcgtc ggccctaaaa agcccatttt gtttgtcgat ggccgttaca ccactcaggc
    39181 gggaatggaa acggaaggaa tcaaaatcat agaatgcgct gatagaatcg aagcagttgc
    39241 gcttatgacc aggcaactga aattaaaaca catcggtttt gaagctgatt ttgtcacctg
    39301 gagcctccat aataaattaa ccgctcaggc tcctgaaaca aaattcatac cctggaccga
    39361 tgaattaaaa acactccggg cgatcaaaga cgatgatgaa ataatgatca tgaaaaaagc
    39421 ggcggagatt tcttcagccg ccatccaatc ggtcgtcaac aggatcacgc ccggctggac
    39481 ggaaaaggca acggcctggc aactggaaat cgccgcccgg gaaaacggcg ccgacggcac
    39541 cgcctttgaa accattgttg cctccggtga aaattcttcc ctgccccacg caaaaccggg
    39601 caaaagaaaa atcaggaagg gggattttgt cgtcattgat ttcggcgtga gatatcaggg
    39661 ctattgttcg gatgaaacct gcacccttgc ttttgggaaa ttgacagaca gacaaaaaaa
    39721 tgcttatcag gtggtgaaag acgctcatga tcgcgctttg gaattactca gggcagacat
    39781 tcccggggcg gcaatcgatc aacacgtccg cagtgtcttc gggaaaaatt atgtccggta
    39841 ttttacgcat ggcacgggcc atggtgtcgg tctggaagtt cacgaggcac cgcgcctgtc
    39901 ttccctttca gacgatattt taaaaccgcg aatggtattt accgtggaac ccggattata
    39961 tcttccggga cgctggggag tcagaattga agataccgtg ttactgaaag aaaacagttg
    40021 cgaaaagctg acaaaaatgg ataaaaggct taccattata gaataaagaa agaggttgtt
    40081 ctatcatgaa ggattttccg gaagatataa tttacagttg cgaacacatc tgggtgaagg
    40141 ttgacggaaa tatggccacc ataggcatca ccgattacgc acaggaaaga ttgggggata
    40201 ttctttccgt cgagtttatc gaaacagata cctatgtgga gcgcgatgaa acattcggct
    40261 caattgagtc cgtgaaagaa gtggtggatt taatttcacc tgtcagcggc actgtcgtcg
    40321 ttgtcaatga agatattctt gaagatgtca gtatcattaa cagagatcct tatgacacag
    40381 gatggctggt cgtgattgaa atggacaatc tcgaacaact ggaagacctt ctggcagcgt
    40441 ccaattacca tgattttata gcgcaggaag aaagcgaata attcttcttt atgggctgga
    40501 gatttctcaa ttaccaaacg aataacattt ttgagaacat ggccatcgat gaagcaattt
    40561 ttcgcggagt cataaccagt aaaacagaat ccacgataag attttacgga tcagagccgg
    40621 aagctgtttc catcggttat tttcaggatg cgaaaaagga tttaaacctc gggcaatgcc
    40681 gccacgatgg gattgatgtt gttcggagaa taacaggcgg gcgggccgtt tttcactgca
    40741 atgaaatcac atactccgta acggcgggaa gcggagaaag aatttttccc gacaacatat
    40801 caggaacata cagggtcatc agcaaatgtc tcgtccgcgg gcttggcaat ctgggcattg
    40861 atgcccgctt ggctgaaacc ggcgatgaaa ggaaccaggg agaaaaagtt gcctgctgtt
    40921 tttcaacatc ggcagagaat gaaattctgg tgaacggccg caagatctgc ggcagcgcgc
    40981 aggtcagaag acggggagga tttttacagc acgggcttct tttttacagg tttgatccgg
    41041 agggaacggc gaatctcctg ctttcctccc gttctccgga gcaaatcgaa aaattaaaac
    41101 aatccgtgac gtcgataaac gaatatctgt gtcaaccgct tacagcggaa gaaatatgcg
    41161 aaacattaaa aaaaagtttc tgcgacgaac tgggaataca tctgggaaaa ggatctctga
    41221 cgcccgcgga ggaaaaactc aaaaacgaat tgatgaaaaa atatacagat ggcttgtgga
    41281 atatcgagcg ggaaaaatac tttaaggcgg tttgattcaa catgatcatt cggaatctta
    41341 tagcaaaagc aatattgaca caaaacggtt attcggcgcc ggaaatcacc gatgcgatca
    41401 aactggatgc taatgaaaat ccttttaccc tccaggaacc gttaaaaagg agactggcag
    41461 acacgctgcg ttcgattgat ttcaaccgtt accctgccgc cggcgctccg gatttacgcg
    41521 aaaggtttgc cgactattac ggcgtaaaaa aaaatatgat catgccgggc aacggctccg
    41581 acgaactgat ccagattctc tgcatggcct tcaggggcaa aatcaaaggc gtgctggttc
    41641 cttctccaac attttccatg tacaaaatca tcgcggtcaa taccggtaat aaagtggtgg
    41701 acgttcctct ggatcaaaag ttcgatctgg atgtggatgc catcgccgcc agaataaaaa
    41761 ataattttcc cgcgttgatt ttcctgagtt cacccaacag ccccaccgga aaccttttca
    41821 gccgcgaaag aatagaagcc gttatcagaa aagcccccgg cgttgtcgtc attgacgaag
    41881 cctatagcgc ttttgccgga caaagcctgc ttccgctgtt gaaaaaatat aaaaatgttg
    41941 tttttctaaa gacgctttcc aaattgggca tggcttccat acgtctgggc tttttaatcg
    42001 ggaatcccga ccttatcgat cagttggaca aggtaagatt accctataat gtcaatgctc
    42061 tgtctcagaa agccgcggga ttttttctcg atcatgaagg agaattcatc aaacaaatca
    42121 aagagataat tcagagacgc caggaactct accgggaaat aaaaaaaatc aaatggattg
    42181 tcccataccc ttcccgggca aattttattt tttttagttg cgcttttgat gccaatcgca
    42241 tatataaaga cctcgtttcg caaggcataa tcgtcaagac gctgaatctt gcgccgcgga
    42301 tgcctgacgg catcagagta acggttggca gtaaaaagga aaatcaagcc tttctcgacg
    42361 ccttaaaaaa cattactcca gaataaggag cgtgatttaa aattagtggg acattgcagg
    42421 gtggattacg gcagcttttt cgctttgccg tactgccctt tttttgttat gaaactcagt
    42481 gaaaataaac gcagtgccgt ttgtggtgtt agtcgaatta tcccaggatc gtcgcgacgt
    42541 ggaatttgag accaattttc agaaacgaag tgcaccgaca cttgctgatc gaatgcctcc
    42601 ggaggggcgg aacgaaacgg gatgtgacac tcgccaagaa caagcacagc gaagcgcaat
    42661 gaaatttgta agtcaaatta tccgatcggc gcaggcaacc ggacacaatc cgccaacccg
    42721 ggacgtatcg gacaagatga ataagcgttg acagaagtca cacgatcact aacaactatt
    42781 aacagggagg aataaatttg gaagtaaaag taatcgacaa tgacgtggaa aaagcactga
    42841 aaatcttgaa gaacaaactt tcgaaaagcg gcttgttcaa ggaactgaaa gttcgccggg
    42901 cgtatgaaaa gccgtctgta aaacaaaaaa gaaaaaccat agaagctcag cgccgcctgg
    42961 ccaaggttca gagacgccgt cgttattaaa ctcatttttt tattcctatt ccataccatt
    43021 accgctcgaa gggaaggtgt cgatctatgg atgaaaagca atatgtcatt gacgctctgt
    43081 ccgtcaacga agcatggcgg gttttccgta tcatggcgga attcgtcgag gggattgaaa
    43141 cgctctccaa tgtcgacaat gcggtcacca ttttcggttc tgccagagtc aaacccgacg
    43201 acatctacta ccagaaaacc gaaaagctgg caaaactcct ggttggggaa agattcaacg
    43261 tcatcaccgg cggcggaccg ggaatcatgg aagcggcaaa caaaggcgcc gcagaagccg
    43321 gggggaaatc cgtgggattg aacattaagc tgccctttga acaaaaacca aacgattacg
    43381 cgaacatcca cattgattac aaatatttct ttgtccgcaa agtcatgttc gtcaaatacg
    43441 cggttgccta cgtcattatg ccgggcggct tcggcaccat ggatgagctt ttcgaggccc
    43501 tgacgctcat ccagacaaaa aaaatcaagt ctttccctct gattctcatg ggaagcgaat
    43561 actggaaagg cctgatcgac tggctgaaca atgcgatgct ggcggaagac aaaattcttc
    43621 ccgttgatat ggaattaatt cagatcgttg atgaaccgga agatgttgtc aaactcatta
    43681 aaaaatacgt tatcgtataa tcgtccttga gcccctgatg cgtcatggaa aaaaccttaa
    43741 ccaatctgaa aaagggcatt gttgcaccct gttatcttct ttacggtgaa gaagaatatc
    43801 tcatcaaaga agccctcaac aaaatgattg ccgttcttgt tccggaggcc gatcgggatt
    43861 tcagtctgtt ttatctggat ggcgagaaca cagagataaa cagcctgatg gatcatctcc
    43921 tgaccccttc tcttctgggc gacacaaagg tcatcgtcgt gcaagacacc acaattttca
    43981 catcgcgtga tgatctggcc aaactcgcag ccaaaatacg taccaccatc aacgaaaacc
    44041 cgaccaaagc ggccagatat tttctaacct tcctcaatat tgcaggattt tccctggagg
    44101 atctttatgg tacgggctgg cagaaaatta cggatgaaca gtggagcggc cttgtcaagg
    44161 gagatgccgg cgacgagcgg gaaaagtggc tgccccgaat tctggatata tgccagacgg
    44221 ccggactgac ggacgcttcc accagtaaaa cggaaaaact ggaagaggtt ctgaaagccg
    44281 gattgcccgc gggcaactgc ctgattttca ccgcagaagc cgtggataaa aggaaatcac
    44341 tctataaaat cgtcgcaaat atcggcgtcg tcaactattt cagcccggtc aagaaggagt
    44401 acgcacgcaa agaaatcctt cagaaagaag cgcaaaaact attggatggc tgcgggaaaa
    44461 aaatggctcc ggcggcatgg attgccctgg gcaaaaaaac cggtttcgat ttccgtaact
    44521 ctctgtcgga acttgagaag cttatttcct tcgtcggaga acgggcgctc atcgataaag
    44581 aggatgtgga agaagcggtg ggcagaacaa gggaggagga aattttcgct ctgaccaatg
    44641 ctctgagcga gaaaaatcaa ctggccgcaa tcagcacctt aaatcatctt ctggatcagg
    44701 gcatccatca attaatgatc atgaccatgc tatccaggga aatccggctc ctgctgcagg
    44761 ccagagtcat ggtcgattcc gggaaactgc cgaaattcaa ccaatccatg gaatacggct
    44821 ggtttcagaa cactgtttat ccggtcttca gcgcgttgaa tcccaccggc agaaaaaacg
    44881 atatcctgat tttaaaccag catccctttt ccgtttttaa cgcactgcgt aattgcgtcc
    44941 gctttacaac ctcccgcctg accgacctgc tggatgaact cctcagcctc gagcgttcaa
    45001 tgaaaacatc cgcctccaac ccgcaactgc ttctcgaaaa ttttttgatt aaattttgtg
    45061 cctgagtttt accggaaaat gccggtacac tgctcgatcc aacgatttga gatgcccgtg
    45121 cgaaaaggtt ttcgcgtcgc cggcactctc gtgattctac aacgacggca gattttgttg
    45181 cggttcgccc agaataaacc ggccgcactc aatccattcc tttaacgtat aggcgatctg
    45241 tctggctttg aagtaactgg aaaggggcgc cgtaatcact ttttttcctt ccaaaacaat
    45301 ggtgccgctg cgcagatcct tgtaactgac ctctgccaga ggttttggac tgcccttggg
    45361 gtaatccatg ctgtaatcgt atacctgggt atagatatcc tcgtctctga cggccgtgta
    45421 acgggccatc tcttcatcca acacaggaat gggaatacca atgcccacaa aaagagaaac
    45481 cccataaccc aagatactgg cacccgtgag ccaatccgca gacatttctt taagattacc
    45541 gatcacggca atcgtcccgg ctccctcccg gggaacaccg ttggatccgc gcagcacctt
    45601 cggattatgc tgcgtgcccg gccaggcgac aaatccctgt gcgccaccca aaaatatgcg
    45661 cgtaccgatc ccgatggttc tgtaataagg atcattaaat aatggactta attgtccgga
    45721 cgtggaatat gccgcattgg ccatttgcgg acgcagcatc cccatgtaag tgtagatcgt
    45781 tttgtcggac agattcacgg cacaattata attctgatac gcattgcggg gattgaacag
    45841 aatcgcatcc ctcaaatctt ttatcgtaat gtttctttcg aatttccggg cgggatagca
    45901 atcggttccg tacccttcgg cacgaagctt gacaaaattt ccggaaacca ggtcctcgat
    45961 gacgtgaccg ccgccgtaat taaattcacc gggataaaca ctgttgagcg gatcgttttc
    46021 ggccacttcc gtcgcgccga tataacaatc cacggcggca ataccgccgt aagcggcgac
    46081 gtcattcaac cacacccggg aagccctgat cttgggagac gtatgtccga aattgagaaa
    46141 cgcccccgag gagcacatcg gtccgaacgt tccggtggtc acaacatcta tcttgcgggc
    46201 ggcctccaca tcgccgtggc gttcgacaac gtcgatcatt tcctctgctg tcaccacgac
    46261 agcacgtcct tctttgatct tttcgttaat ttcttttatt gttttcttaa ctttgaattt
    46321 tttcataccg tatcgttatc gtcacatcgt cattgtccat tttcatacca ctctttgttt
    46381 ccaattgcaa aagctaagaa gcgttcaggt atggccgaac ccgccgctgc cccggactgt
    46441 ttttgccagt gtttcggttt cctcccaatc cacccggcag acgcgctgca ccaccatctg
    46501 tgccagacgc atgcctcgtg tgacgataaa gggctcttcc ccatgattaa tcacaatcag
    46561 accgatttct ccccggtaat cggcgtcaat cgtccccggt gtatttaaca gggtaacgcc
    46621 gtttttcagc gccagtccgc tgcgcggcct gatctgcgct tcatagccat cgggcagagc
    46681 gacggcgatg cctgttgcga ttttttttct ttggcccggc aaaataattt cttcttcagc
    46741 caccgccgaa aaaatatcca tgccggcggc ttgatcggtc atgtactgcg gcagggggat
    46801 atcatcgttt cccggaagtc tctgaataca aaccttgact tttttcatgt ttcgaccaga
    46861 aataattatt gggagaactg cttacaatgg ttgataaaga gatccgttcc gcaccaacgt
    46921 atcgttgcat gcggaacggc ttgtttttcc ataaccctta accgggaatg tattacttga
    46981 cttccgcgcc cagtgcttct ttgcggctga ggcggatctt gccggacttg tcgatttcca
    47041 gcaccttgac catcacttca tcgccttcct gtaaaacatc ggttaccttg gcgattcttt
    47101 ccttggcgat ctgtgaaata tgcaacaggc cttccgttcc gggcagaatt tccacaaacg
    47161 cgccgaaatc gacaatcttc ttgacggtgc cgcggtaaat ttttccaatc tcggcttctt
    47221 ccgtcagcca tttcaccatg gccaccgcac gggcggctgc ctcggcatcg gcggaggcaa
    47281 tggtgacggt tccgtcatct tccacatcaa tcgtcacacc ggtctcactg ataatctgac
    47341 ggatgttctt gccgccggaa ccaataacgg tgcgcacctg atcttccttg accttcaccg
    47401 tcgtgatacg gggcgcatat tgagaaatgt cgcctctcgg cgcagccagc gtctcacgaa
    47461 tcttaccgat gatgtgcatg cgtccctctc tggcctgagc cagcgccttg cgcagaatat
    47521 cctcggtcaa gccgtcaatc ttgatgtcca tctgcatggc ggttacgccc ttttccgtac
    47581 cgcatacctt gaagtccata tcaccggcat gatcttcatc gcccagaatg tcggacagaa
    47641 taacgatctc attgccttcc ttgaggagcc ccatggcaat gcccgccacg atatctttca
    47701 cgggcacccc accgtccatc aggcataaaa taccgccgca gactgtggcc atagatgacg
    47761 aaccgttgga cgacaaaatt tccgaaacaa tccggatggt gtagggaaat tcctccggag
    47821 acggcagaac cggcaccaga gctttgcgcg ccagcgctcc gtgtcctatt tccctccgtc
    47881 cgggactgcg cagcatcttg gcctccccca cgctgaaggg aggaaagttg tagtgcaata
    47941 aaaatgtccg gatctcttct cccccgacgt aatccatccg ctgctcatcg gcggatgtcc
    48001 ccagcgtcag cgccgccagc gcctgtgttt ccccccggct gaacagtgcg gaaccgtggg
    48061 cgcgcggcag cacaccggcc tcggaagaaa tcggacgaat atcaacggaa gagcggccat
    48121 caattctttt cttgtcctga atgatcatat cgcgcaggat gcgggattcc aggttctcga
    48181 taatgccggc aactttttta ctcaggacgg cgtcgtctcc gccgatattt tttataacct
    48241 cggcgcgaac gtcgcccagt ttgctgtaac gttccagctt gcgcggcatg gcatacccct
    48301 ctttcaagcc gggcagggct tcttcacgca cgcgagcaac aagctcttca tccggaaccg
    48361 gctcctcaac cggcctttta accttgccga tagatgcctg aatctgatcc tgcaggtcaa
    48421 taaccggccg gatcgattcc agcccgaatt taatagcatc gacgatgata tcttccggaa
    48481 cctctttggc ttcaccttcc atcatgacca gttccacgtc atagggacgg ccggacgttc
    48541 cgggtgtcac ttttcgtccc accagaaaaa ggctcatctc gctttccttc atcttctccg
    48601 ggcacatatt ggcgacaaat tcgccgttta tccggccgac acgcacgccg gcgatcggcc
    48661 ccttgaaagg aatgtcagat atccccagag cggcggaagc gccgatcagg gacgcaacat
    48721 cgggatcgtt ttctttgtcc acggataaaa gcgtggccac caactgtgtt tcagaaaaat
    48781 atcccttggg gaataatgga cgaatcgcgc gatcaataac gcgcgatgtc agaatttccc
    48841 tttcgttggg acgtccttcc cgtttaaaaa agccgccggg aatttttcct gcggcaaaag
    48901 tcatttcctg ataatcaacc gtcagcggca gaaaatccac gccttcccgc gtgcttttca
    48961 gagaaaccgc tgtaaccaat acaactgtgt cgccgtagta aaccaaaacg gccccatcgg
    49021 cctgcgcggc cacataattc gctttcaggg aaatgttccg cccggcgaaa tccgcactga
    49081 atacattact cattcatttt cctccataat tgtttgtgag tgctggtctt ttcaaattca
    49141 agggcgaaac gcataaagca acaaccgttc aacgatcgat tcgcttacgg tcagcttgcc
    49201 ttatgccttc cgcccttgaa taattctttt actttctgag attcaaacgt ttaataacgg
    49261 ttctgtatct ctcgatgtca ttttctttga tataattgag aagccttctt ctctggccga
    49321 ccagcttcag cagacctcgg cgcgaatgat gatctttctt atgcgccttg aaatgctccg
    49381 tcaaatactc gattctggcg ctcaaaagag cgatctggac ctcgggagac cccgtatcct
    49441 tttcatgcag ccgataatta tcaatgactt ctttcctctt ttccgaactt aacacgcgtt
    49501 ttcctccttt ttacaattta taatctttta tgttggtttt tatgttcttc attcacggct
    49561 gattaaacac ccggagaatt ctggccgcct gcctttttcc gtcaaaagca gccagctcgc
    49621 tgaccggggc cagcatttcc gcaacagcca gaagattgcc gcgactgacg aattttatca
    49681 tatctcctgc tttaagcaag ggaagatcgt gctccttgag catttccacc gaaggctgcc
    49741 agccgttgcg cagttttacc gcgcattgat cttccagttc aatggtggcc agaagcggca
    49801 gaagctcagc catcggcaat attttttcag ccagtttctc ttttgcttcc gggaacttat
    49861 aatcatttag attaacggcc atcgcctctg aaaaaatccc gctttgcaaa cggcgcagcc
    49921 cgctcagaca ggcccctgtt cccagcgctt ccccgatatc cgcacaaaga gaccggatat
    49981 acgtcccttt tgaacaggcc acacggaact tcaccgacgg aaaagaaata ttctccacaa
    50041 gcaggctgaa aattttgatt tccctcggct tcaaatccgg gaaaacccct tttcttgccc
    50101 atttatacag gggcttgccg caatgtttaa ccgcggaata cgccggcggg acctggttta
    50161 tggttccgat catccgcatc atgaccgatc gaatttcttc cccagacaca accttatcgg
    50221 attgtcccgt aatgtttccc tcaatatcca gggtgtccgt tgtcacaccc agcaacatcg
    50281 tggcccggta ttcttttttg tcgcccgcca gaaaaccggc cagtttggtg gcttcattca
    50341 cgcaaacagg cagcacgccc gtagccaggg gatccagcgt tcctgtatgg ccggcttttt
    50401 ttatccccag agctcttctc acatccagaa caacatcgtg cgaggttttc cccgccggtt
    50461 tatcgatcac aataacgccg ttcatcatta acactacaat tccctgaccg cctgaataat
    50521 ttgcgatttg atggtttcaa tatcgccgtc catccagcag gacgaggcat gaatatggcc
    50581 gccgccgccg aaccgtttgg ccactttttc cacgtcgacc ttggctttgg aacgcaaact
    50641 cagcttgaaa tttttttccc ctctctgggt atagagaacc gatacttcga tacccttcac
    50701 cgtgcggggt atatcaacaa atccttccgt gtgctcccag gcggcgcccg tttccagcaa
    50761 atctttctgg gtaacgacca tggacccgac cttgctttcc agatcaagcg ataacgtcga
    50821 cagtgcccgg gccatcagtt tgagccgggc cggagaatcg ctttcataaa tattttccgc
    50881 aatgcgctgg ggactcgccc cttcccccac cagcaccccc gccgccatga acgtctcctt
    50941 tgtcgtgctc gaatagcgga aactgccggt atcggtaatg atggccgcat acagattggt
    51001 gcagatgtct ctggacattt tgaagcgcat gtcctgcatc aggcggaaaa cgagttcgcc
    51061 ggtggaactg gctttggcat ccagcatttt taaagcacaa aatccgttat tggaaacatg
    51121 gtgatcgatg ttaatgagcg tcttgatttt tctgatattt tccgcttcat cgccgaccct
    51181 cgacaaatcg ctgcaatcca gcacgatgca ggtatcgtac tgctcgatat tcgttaacgt
    51241 atgagaaatg ttgtgcgtgg ccggcagaaa ctgataacgt tccggcgtgc gatccttgtt
    51301 atagacaacg gccttcttgc ccagttcttt cagcatcaga taaacggcaa gttccgaacc
    51361 gagcgcatct ccgtccagcc gcacatgggc ggtaatcaga aagttctgtc cttcgtgaat
    51421 cgcttggata atatcatgaa tcattttcat tttcctgact gtggatctgt gccaacaatt
    51481 tttcgatacg gttcccgtaa ccgaaagacg tatcatgaat aaacatcagt tccggcacaa
    51541 aacgcaactg caatttcttg cccagctccc ggcgtatgaa tccgcttgcc tgagccagtc
    51601 ccgctttaat ttcaacagag cattcgtctt tgcccatttc cacgaaataa atcttggcat
    51661 tgcgcaaatc atccgacacc ttgaccgccg taatggttac cgaatgcaga cgcgggtctt
    51721 tcacgtcctt catgattatt tcggacatat ccgcccggat taattcggcg acccggtccg
    51781 ctcttttaaa attcatcgta cttctgtata tccccgtcgg ctgtttccag aaaactcgaa
    51841 acaaccataa tttccatttt gcagtttaca atctcggcaa tctgcagatg atcgataaaa
    51901 cgcagcagat gatccgcctt cccgttgata taacgggaat cgttgccgac aaccgcaaaa
    51961 gaaatttcgg ccagcttata attatcaaga tggcctgttt ccgcaatgga aacattaaat
    52021 tcattttgtg cgcgttttaa aattcggttc aaaacgcttc gcttgctttt taaagagccg
    52081 ctttccggaa ttctgagatt aatgatgccg tatccgacaa ccataagcat tctccggaaa
    52141 cctgaagcat tcgggagccc cgattttttt agaaccgttc ggatataaaa aaactatcgc
    52201 ttatcagcat tcggttccag tttcttttcc aacgtttcta tcgcatacgc ctcaatgaca
    52261 tctcccggat gaatatcatt aaatccttcg atgccgatac cgcattcgaa accggccagc
    52321 acttcccggg cgtcatcctt aaaacgtttc aatgacagaa tctttccgtc aaaaacgacc
    52381 acgctgtcac ggacaagctt gagattattg ctgcgcatga tttttccatc cgtgacataa
    52441 cacccggcaa tggtaccgac cttcggcacc ttgaaaagtt cgcgcacttc cgctctgccc
    52501 tgaacaacct cccggtattc aggttccaga agaccttcca tggccgcccg cacatccgca
    52561 atcacattgt aaataatatc gtagagcttg atttccacac cttcccgctg ggcgacatcc
    52621 accacccgcg catcgggtcg cacattaaaa ccgataataa tagcctggga agccgacgca
    52681 agcatcacgt ccgtttcgct gatggccccg gtggaactat gaataatctt cagcttgata
    52741 tcgtccgttg aaagtttatt caatgcgtcg gaaatcgctt ctatcgaccc ctgcacatcg
    52801 gccttgacaa taacgttgaa ttccttcgcc ccttctttta tcctctgata aagctgttcc
    52861 agggtgatct tggacagaga cgaaagttcc ttttcccggg cctttctggt ccagtaatcg
    52921 gcaatcgcac gcgctttctt ctcatcgtcg acgcagaaaa attccgctcc cgtctgcggc
    52981 acactggaaa atccgatcac ttccaccggc atggacggtc cggcttcctt gattcgccgt
    53041 ccctgatcat taatcagcgc gcgaacacgg ccgaattcgg tcttggaaac aaacgaatcg
    53101 ctttccctga gcgtgccttc ctgaatcagg acggtcgcaa ccggaccgcg tcccttatcc
    53161 agtttggatt ctatgataat gccgcgaaca gggcgatcgg gatcggcctt cagctccatg
    53221 acatcggcct gcaggagaat catttccagc agttcttcaa tgttgatttt tttcttggcg
    53281 gatacttcac agaaaatcgt atccccgccc cactgttcgg aaagcaaatt atgttcggtc
    53341 aacgcttgtt ttattttttc aggatccgca cccggcttgt cgattttatt aatcgccacg
    53401 ataatcggaa cgccggcaac cctggcatga ttgatggcct cgacggtctg attcatgacg
    53461 ccgtcatcgg cggccacgat cagaacaacg atatcggtaa cctgcgctcc gcgggcccgc
    53521 atcgctgtaa acgcctcgtg accgggggta tccaggaaca caatatcccg gccgttgaca
    53581 tgcacatgat aggcgccgat cgcctgcgtg atgcctcccg tttcaccgtc ggaaacattg
    53641 gtctgccgga tggcatccag cagggaggtt ttgccgtgat cgacatgtcc catgatggtc
    53701 accaccggag cacgcggttt gagattctcc ggtgtggagg gagccttcag aacggattcg
    53761 tcaaattcca cgtccgcgga ttccagctgg aaactgaatt cacccgcaac aatgctggcg
    53821 gtatcgtaat cgatggcctg attgatggtc gccatcacgc ccatggacat cagcttattg
    53881 ataatctcgc tgaccttgat gcccatgcgt ttggccagtt cgccaacggt aatcgtttcg
    53941 ccgattttga tccgtcgttt aatggccttg ggaacggtaa tctccgtttt cttcatcttg
    54001 gtcggcgcgg cctttttccc ctcacgccat ttggtaaaaa caacttcctg atcctcttca
    54061 tgccgtttga gccgcttttc gatcttcttt tccaggattt tcttggtcgg gatcttcttc
    54121 tcttcttcga tcaagacctc gacctgatat ttgccttttt tcttcggctt ttccgcagac
    54181 ggttgtgata ttttttccgc cggtttggca gccggcactt tggggggaac cgcctttcct
    54241 gtttccggcc tgataatttt atgtttggta atgatcgtcg gcttgacagg agcaaccggc
    54301 gcttcctttg tttccggttt gacagctttt tcctctgccg acttttcaag gggcgcggct
    54361 ttctgttcca aagaagcctt cgtttcggcg gcagccggtc caaccgctgc cggaggaacg
    54421 acctccttca ccgctttttc cgaaaccgtc tctgcaggaa cagcaggctc ttccccaggc
    54481 ttttcctttt taactttttc agtttttccc ggaggcgctt ttttaagctt gtcttcggca
    54541 ggttcttttt ccggaggttt ggcttcctca agggggactt cttcaggcgg tttctccaca
    54601 gcggtcacga ccgtgcggcg tctgataacc gtggtcttga tcctctgttc cacgacttca
    54661 cgaagttctc cggcggtcag ctctttcttg gcccgctccg catcagcctc ttccagagaa
    54721 gccgacgccg atttaaccga aatgccgatt ttttccaaac gagcgatcag aaccttgctc
    54781 tccattccaa gctctttggc caattcataa acccgctttt taggcatgcg caacaatctc
    54841 catgaaaaat ttcttaattt tcagatgatg aatcactctt ggaaccagcg atctcctcgt
    54901 ttaaaatttt accggccgtt tcaatccact gctgggccgt cttttcgcct atccccggaa
    54961 tctgagacaa caattccggt tccgtcgccg ccaccatggc gatactcttt atgcctttgc
    55021 tgaacagttt ttccgccatg gccggaccga tgccggtaat cttggtcaga tcattctgtc
    55081 cgctttttcc ttcctcctga gcctgctcca ttgtttcgct cttcatgtca atattccatc
    55141 cggtcagttt gaccgccagc cggacattct ggccgttctt gccaatagcc agagaaagct
    55201 gatcatccgg aacaatcaca gtcatggtcc ggttttcatc atcaataaaa atacggttga
    55261 ctttagcggg agaaagagca ctgctgacat atttggccgg atcttcgcta tagggaatga
    55321 tatcgatttt ctcgccgcgc aactcctgta ctacgctttg cacgcgggac ccccgcatcc
    55381 ccacgcaggc accgacggga tcaatatcct tgtctttgga tttaaccgcg atcttgcctc
    55441 tttttccggg ttcccgcgcc acattgataa tatcaatcaa cccttcggaa atctccggaa
    55501 cctccacctc gaaaagcgcc ttcaaaaatg aaggatgggt gcgggaaaga atgatctgca
    55561 atcccttggg gctctttttt acttcaacga tataggcgcg gattcgttcg ccgcgtttgt
    55621 aagcttctcg atgaatctgt tccgaaggag gcacaatgcc ttctgcgcgc ccgagattga
    55681 cgacaatgct gccgccttca aatctctgga caaagccgtt gaccagttcg cctctcctat
    55741 ccttgtattc atcatagata ttgtcgcatt cggcgtcttt cactctctga atgatgatct
    55801 gcttggccag ttgaacggca atccgtccga actgactggt ttctattttc atgcctagac
    55861 tgtcgccggg ttccgctcct tcatcgagcg ttgcccttgc ttcctctttg gaaatttgtg
    55921 tttccggatg caaaaccttg tccacgaccg ttttgaactg gaacacctcc acttctccgg
    55981 tctcatcatt ataatgggct tctatttcca cattctgtcc gaatttttta ctggcagcag
    56041 taagcattgc cgcttcgagg gcttcgacga taatttcctt gcttattcct ctgtccttac
    56101 ccatctgctc gatcagacgt ttaagttctg gcaacattta aaagttcctc ccttttttct
    56161 tcgctgatat attagaatct cagcctgccg gcattaattc ctgtaaaaat ctatttcttc
    56221 tcatcggcaa gattggcttt taaaatttcc tgccgcggaa tccggtaaac tttgcccgaa
    56281 acttccacca aaataagttt ccggccgttt tcttcgatat aatccaacaa aacgccgtga
    56341 aaatttttta caccgtcgat cttgatgccc gtttttacat ccacgcgcga attccggtat
    56401 ttcaaaaaat cctgatcgcg cgatatcggc cggtcgaatc cgggcgacga aacctccagc
    56461 gtataagaac cgttcacgac ttcgcgaatg tcaaaaatat cacctaactg attgcttata
    56521 ttggtacaat cttccagtgt aatgccgcct tccttatcca gaaaaagacg gataatccag
    56581 cgcgtatgca tctttatgca ttccacgtga atcaattcca tgccttcaga agcaacaacc
    56641 ggctcggcta aaagccggat tttctcttta acatcaaagt ccataatcgc tcagaaaata
    56701 aaaaagtggg cacgggccca ctcatcaata ccactttatc gatgatttcg tgtttttgct
    56761 agcacatccc tatggacaaa gcaagtaaaa tattaaggca gattaaagtc gtttttatca
    56821 atgatattta taaattatcc gccttattat tccctttgtc ctgccaatcc cgacaagtcg
    56881 tggattggcg ggacaattca aattacccat ttcagtgcgc tacgctttcg aaattggagt
    56941 ttcattggag cgggcgaccg ggctcgaacc ggcgacgtcc agcttgggaa g
//
LOCUS       DAOSME010000007        54320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_777_length_54320_cov_20.562738, whole genome shotgun sequence.
ACCESSION   DAOSME010000007 DAOSME010000000
VERSION     DAOSME010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 54320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54320
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_777_length_54320_cov_20.562738"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLG98_01895"
     CDS             complement(<1..439)
                     /locus_tag="PLG98_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOE32030.1"
                     /translation="MTLLNKTPADGGLKKTVVAVGGMTCAACVRRVENAIKEVIGVQD
                     AVVNLANGHATVMHSTQWEGPAALARVVAEQGYEYLGEIKNHLADPIEASRLKELREL
                     KLKVACGAVLSVIIFFGSMQHWFPFLDFIPHRIMLLLLFVLTAP"
     gene            complement(423..623)
                     /locus_tag="PLG98_01900"
     CDS             complement(423..623)
                     /locus_tag="PLG98_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOE32031.1"
                     /translation="MKSIKIKGMSCGHCVMAVTKALNAVDGVRNVEVDLKNGVAIYEE
                     IKPVDPTVVAAAIKKAGYDVVE"
     gene            849..1691
                     /locus_tag="PLG98_01905"
     CDS             849..1691
                     /locus_tag="PLG98_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE32032.1"
                     /translation="MIIRCWGSRGSIPVSGRLYQRYGGNTTCLEIRTKDNNILIVDAG
                     SGIREAGNALLAEGRLNFTLLLTHAHWDHIMGFPFFKPIYSSKTRMDIRGCPFAQTSI
                     KKMLSRIMAAPNFPVNYSHIRAKIVYHETCFKPVYRLGDMLITPIALSHPNGGTGYKF
                     EEDGKTFIFLTDNELGHRHPGGLPFRQYLAFCRNADLLFHDAEYKEAEYKKTRGWGHS
                     SYKDVLKLSLGARVKKLGLFHHNQERTDPDVDAILKDCRDTVKKEGGKMNCVAVGQSM
                     QFTL"
     gene            1722..2114
                     /locus_tag="PLG98_01910"
     CDS             1722..2114
                     /locus_tag="PLG98_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32033.1"
                     /translation="MLFLLHIVLMVIATLGIMAGVSAAMFFRKKKNWLKIHKTLNTLS
                     LAGITAGIIMAFFYVAGTGGKHLNDVHQMTGLAAFTVVLIAIALGHYQFKALNKSAIR
                     AAHRWMGRVSIFILAAAAFLGLRLINLL"
     gene            2150..3847
                     /locus_tag="PLG98_01915"
     CDS             2150..3847
                     /locus_tag="PLG98_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOE32034.1"
                     /translation="MFLPTTRDELKKLKWDKPDIILVTGDTYIDSPFIGVSVIGRVLV
                     DAGFRVGIIAQPDWHTPDDISRLGEPRLFWGITGGCMDSMVANYTATKKKRKNDDLTA
                     GGRNNRRPDRAVVVYANLIRRYFKETKPLVLGGVEASLRRIAHYDFWSDSIRRSILFD
                     ARADVLVYGMAEKAVVELAQALRDGKDTTDIRGICYLAPAPPPQNALELPAYEDVLAD
                     KKTFIRMFNRFYCNSDPLTAKRLYQKHGDRYLVQNPPQPHLTTPELDHIYGLDFTRDV
                     HPFYSARGNVRAIETITFSITSHRGCYGECNFCSIGLHEGRTVISRSEKSILDEAQKL
                     AGRSDFKGYIADVGGSTANMYGIECAKKIKKGACNGKRCLSPRVCDALPADHSRQINL
                     LRNLRNVRGVKKVFVASGIRYDLLLNDRAHCLAYMKELVEHHTSGQLKVAPEHSEWPV
                     LKLMGKPQAQALLNFKHLFEKTSAVSKRKQFLTYYFIAAHPGCTEEDMRRLKAFTTRE
                     LKTNPRQVQIFTPLPSTYSALMYFTGIDPSTGKKIFIEKNMEKKEKQKNILIGNKRS"
     gene            complement(3877..5661)
                     /locus_tag="PLG98_01920"
     CDS             complement(3877..5661)
                     /locus_tag="PLG98_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32035.1"
                     /translation="MPNKINIPPDKQAGMIYALLAIAVLAVFSQLIRYEFVIIDDPAY
                     VIFNRHITTGMTPDSFIWAVSTKYADLWHPLVWLSFMLDYRVFGLNAGGYHLTNLLLH
                     MMSSLMLFWLFHRMTGALWKSAFIAAFFALHPLHVESVAWISERKDVLSAFFWMLTLC
                     LYVVYTEKPVIQRYWPVVLGFVCALMSKPMVVTLPVVLLLLDYWPLRRFVSAQKNLLR
                     QIKEKSPFFLLSLLVVIITLYDLKASDIKQYPLDLRLANAVVSFAAYLIKTFWPVTLS
                     IFYPFPDSIPGLRLAGSVIFIAVVSVAVVRMRRRHPYLLAGWLWYAVTIFPVIKIVQI
                     SSDAMADRYHYLPSVGIALMTAWGIPLFFKEESYRKKIMFPAAVLFLAMLALGSWRQC
                     GFWQNSVTLLEHAVKVQKDVYVLHEYLGHAFYFEGKSQEAIAQFNQAVLLNPGYARAY
                     VNRGNVYAATRNYDGALKDYQTALRLHPDEVDAHYMRGMVYSKTGRYQQAIDDFNRTI
                     ALDQNHARAFHDRGLAYFHLGRYEQAISDYDEAIRRKPDFTLAYHNRAYGYFRQRNDV
                     RGCPDAHRACALGDCDLLTAVQQEGRCR"
     assembly_gap    5998..6097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6098..6739
                     /gene="amrS"
                     /locus_tag="PLG98_01925"
     CDS             <6098..6739
                     /gene="amrS"
                     /locus_tag="PLG98_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOE32036.1"
                     /translation="VIAFTYNEPTVFTEYLTDIARNARQQGIRCVMISCGFMNEAPLA
                     EMCETLAAIKIDLKGYSEDFYRSACQAELKPVLRSIRQIAKSRAHLEIVNLVVPTLND
                     SDKMLKGLIDWVMGEVGPDVPLHFSRFHPDYQLRNLPPTPVATLERAYRMARDRGLHY
                     PYVGNVPDHPGNNTYCPKCGKIVVKRQGFFITGMNLRNGLCTFCKNKIAGVWT"
     gene            6756..8294
                     /gene="amrB"
                     /locus_tag="PLG98_01930"
     CDS             6756..8294
                     /gene="amrB"
                     /locus_tag="PLG98_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOE32037.1"
                     /translation="MKWLRLLCFLPVIVFFLTSACQANKDAAAGVREPAFAGKFYPAS
                     ADKLRLTIEKYLQDAVLPKVKKPVAVIVPHAGYIFSGQICADGFRQAGHHPYDVVVIL
                     GTNHTSPGLQGVSLYPGEGFRTPLGIAAVDKTVIASLLTESPVDCMLDKSAHVSEHSI
                     EVIVPFVQIVFPNAKIVPAVVGTQDRHTSIRFGQALAKVLKNKNALIVASSDLSHYPS
                     RDNAVKVDRRTLDAVATLDPAVFQKTIQDTMSKNIPNLSTCACGEAPIIAALAAARLL
                     GADRGNVISYANSGDVSVGDSAQVVGYGAVVLTAGQKGISSSQTDLQKTAAVPFDLGT
                     ADKKALLAFARETLSRFFTTDTVPLARHFPAHLNHPRGVFVTLKKNGALRGCIGHLIT
                     DEPLAKLVGAMALQAAFNDGRFPPLTAEELPDVDIEISVLTPMKQAARTSDIMVGRDG
                     VVLSKNGRSAVFLPQVALEQGWSRDDMLDHLCSKAGLEKRCWRQDATFSIFQAIVFSE
                     SQLK"
     assembly_gap    8370..8469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8470..10613
                     /locus_tag="PLG98_01935"
     CDS             <8470..10613
                     /locus_tag="PLG98_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32038.1"
                     /translation="SIGLISILGQVILLRELNVSFYGVELIYLFALGIWLFWTAVGVI
                     AGRRMALPSRHSPAVLFILFGMILFLDVAFIRSSRLIFGGVPGAYLDFFQQLLVATLS
                     ILPVGLLSGLLFQQTAGLYVDSGRTLAAAYAVESAGGLIGGLFSTLLIILDTQNLFAA
                     LICSIVSFATPLLGAGKSLGAFLRRTAIVLLAMFAGLIFNASPLHDISTRWNHPNLLD
                     SSDSPYGRITVTRMFDQISVFENDALSFETEGTDAEYFCHLVMLQHPHPRKVLILGGG
                     MEGIIGEALKYGPDHIDYVEINPVMTKLVMKHLPEAIRQSLQSPEVRIIYADPRRYLT
                     NSGTYDLILVGMPEPSSGQANRFYTQEFFKQCAEKLKSGGLLGFKLQTAENLWTGPLI
                     GRNASIYHALRSVFPAVLFLPGTTNIVTASFAPLPDTPDIMTGRLQHLQLETRLISAP
                     YIRYLFTNDRFAAVKNLLNEKKDLLNSDIRPVCYQYALIIWLSKFFPRATLFDFSSIM
                     EKTLLRYLPVCFLMVLPVLFLASRFRPGLRRKILVAAAGFAGIALETILLLYFQLKYG
                     VIYRDIGLLLMSFMAGLALGALAVDKLMARFADHRLGTRRCGIILVAGSCCLCAITAL
                     LIRNSLPAGLWLTGLLLTATGFIVSGIFAYASLYEIDDQRAIISPLYAADLIGGCLGS
                     VLVSLIFIPLAGMDTTMWGMLLLSVYAFLLL"
     assembly_gap    10687..10786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10787..12655)
                     /locus_tag="PLG98_01940"
     CDS             complement(10787..12655)
                     /locus_tag="PLG98_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOE32039.1"
                     /translation="MLPAILGISAYYHDSAAALLVDGRIVAAAHEERFTREKHTSVFP
                     VHAATYCLREGGRHLADLDGVAFYEKPYLKLERLLETYHAFAPRGLKSFMAAMPVWMK
                     EKIFMRKMLHDGIAGIGPGRVKLLFPEHHLSHAASAFYPSPFDQAAILTIDGVGEWAT
                     ATIGLGEGRSIKILRELDFPHSVGLLYSAFTGYCGFKVNSGEYKLMGLAPYGHADSGQ
                     TALYRRKILDHLVDIREDGSVLLNMDYFDFATGLTMCRDEKWEKLFGIPRRQRESEIV
                     QSYMNMALAIQQITEDVVSRMARTARKLTGSNYLVMAGGVALNCVANGALLRQKIFDD
                     LWIQPSAGDAGGALGAAFAAWHVGMEQPRKSVSEQDAMAGAFLGPEFGSDDIKRTAKT
                     YQASFVHYEDFDELCAVVAERILRGLVVGWFQGRMEYGPRALGNRSILANPCLPDIQK
                     KLNLKIKYREEFRPFAPAVLEEDVAEYFDLDRPSPYMMLVAGLKEHRRRPQPENERQM
                     TLYERLYHMRSDLPAVTHIDYSARIQSVSKRTNERFWKLIKAYKQKSGWGLLVNTSFN
                     VRGEPMVCTPEDAYRCFMQTDMDCLVMGNDLFSKDDQPPWSEKNPWLASGTPYAKD"
     gene            complement(12665..12814)
                     /locus_tag="PLG98_01945"
     CDS             complement(12665..12814)
                     /locus_tag="PLG98_01945"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOE32040.1"
                     /translation="MDFFRDVWGFLKVRKKFWLLPIILMLLLCGALMIFAGGSAVGPF
                     IYTLF"
     gene            12838..13506
                     /locus_tag="PLG98_01950"
     CDS             12838..13506
                     /locus_tag="PLG98_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32041.1"
                     /translation="MFDIIRPEFVLAYFENRMLIFFPFFQLQRIVAEASPDKTDGQHK
                     NQKNQEQDQPGHHSAEDVRNAQPDHRPRLEYPGPNDIQKKQQQSKTVKKRYIRKKTCY
                     QTKQRGSHAGIFGLIGCDCVGNKFHFVFVIFSETYCDFNITSRSGKPLSSSWQRLFPH
                     RRLPQGAMIRNGHPKECCWSVLKIIHSGIPATGSPDDVRPGNDRFDFAKTAANACLDF
                     LDGS"
     gene            complement(13483..13665)
                     /locus_tag="PLG98_01955"
     CDS             complement(13483..13665)
                     /locus_tag="PLG98_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32042.1"
                     /translation="MSFFIVLFEDGSLQIYQSMQDREFLPEGARQFKCSSNVTVQDLC
                     LWLDGGCKKLRTIKEI"
     gene            complement(13701..>14029)
                     /locus_tag="PLG98_01960"
     CDS             complement(13701..>14029)
                     /locus_tag="PLG98_01960"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HOE32043.1"
                     /translation="QVEMKGMPQSMPPTTFRQCITKSNPVPQNKDKNFECKIISQKVS
                     GGTVSYQVECTGKEGVVQTSGTTTYTDKAMNGASTTSFKMKGQPAMTMSNKIKGKYIG
                     PCPKAQ"
     assembly_gap    14030..14129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14430..16007)
                     /locus_tag="PLG98_01965"
     CDS             complement(14430..16007)
                     /locus_tag="PLG98_01965"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOE32044.1"
                     /translation="MSGEKNTTQDLILSVDAGTQSIRAALIDVRGNIKAIVKTAIQPY
                     FSEHPGWAEQRPDYYWDVFCKTTHQLLQDQEVLKKHVKGVTLTTQRATMVNVDQNGNP
                     LRPAIIWLDQRKADSRKILPAPLRPLLKTVNLLDTLENVIQECEANWICQHQPDIWEK
                     THKYLVLSGFFTHRLTGEFVDSTGNNVGYLPFDNKTYQWAGKFDFKWRIFKVEKEKLP
                     RLVKPSEMMGAITKKASAETGIPEGLPVFAAGSDKGCEILGSGCLTPETGCLSFGTIA
                     TFDVATPKYVELHPMIPPFPASVPDTYYTEVSIFRGFWMVSWFKEEFGLQECMLAEKT
                     AEPPEKFFDSLIQDVPAGCKGLMLQPYWTPGREVDAFAKGSIIGFGDVHSRAYLYRAL
                     LEGLVYGLREGGELTQRKTKIPVTRLKVSGGGSQSDAAMQITADIFGIAAERPHTFET
                     SALGAAIDAAVGLKLYSDFASAVTNMTRTGRVFEPDKDNHGLYDNLYRRVYLKMYERL
                     KPLFRDIRDITGYPAGN"
     assembly_gap    16154..16253
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16254..17898)
                     /locus_tag="PLG98_01970"
     CDS             complement(<16254..17898)
                     /locus_tag="PLG98_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE32045.1"
                     /translation="MISHYFRPNWTEKAPAAGTYRSIFKYGDLHSFKHPSDAWYRMIK
                     EEFHLNDKDFASRVKEGLEPVSLNRAQALKPQQMDCLRNIVGGENVALDDYSRVKYAS
                     GKTTEEMLELRLGIIREVADVVVHPRDKQDVQKIVQYCHQENIPITVYSAGSSVNFGC
                     RPVRGGVSLVVSTHMNKLLEVNEFNQTARVQPGMFGPAYEEGLNKAPELFKTKHRYTC
                     GHFPQSFEYSTVGGWVVTLGSGQASTYYGDAYDIVFSQEYVTPAGTFKTLDYPATATG
                     PKVNDIMKGSEGSFGILVEVTMKIFRYMPENRRRFSFMYPTWEAAVNASREIMQSEFG
                     KPAIYRISDPEETDRGLKLYGMPDFVDKILVMKGFKPMQRCLSLGNAEGDCDYTKLVV
                     RKIKTVAKKHGAISLTGYAAKKWEHTRYTEPYMREDLGDYDILIDTLEAAVTWDNLHR
                     LHQGVRAYIKNRPQTMCMTHASHFYPQGTNLYFIFIAHMKDPEEFRKFQRGIIDNIQK
                     HGGSLSHHHGVGRMIGPWMEKHLGKEQLDVLRTLKKHFDP"
     gene            complement(17948..19594)
                     /locus_tag="PLG98_01975"
     CDS             complement(17948..19594)
                     /locus_tag="PLG98_01975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOE32046.1"
                     /translation="MKRFIENYTGEKFDVAVIGGGISGASVAYEAATRGLKVALLEKN
                     DFSWATSAVTSKMIHGGLRYLVNGEVGLVRESLRERRVLENIAPNFVYPWPMMMAHYK
                     KPLKNNKWVVKVGMLVYDVLSYDKNFTWDPCKKIPVHRTISARQVLQDEPHVRAEGLT
                     GASIFYDCTSLFPERLTLAFIKSAVAHGARAANYARVEGFLMDGAKHVLGVKVKDLFS
                     GKMHNVPATVTINCGGPWADIVLGMAKPDGQVNATLRRSEGIHIITSKKLLTGNYTIG
                     SMTPEGRHFFLIPWRNHALIGTTDKPFNGNPDDYRVTKESIMELIADVNSSFGDGKLS
                     YADVKYTYGGLRPLIEKETSETYSSSRKYEIYDNKDDGLDGLITVEGGKYTTSRKLAE
                     NCLAIVAKKMGRNLGRSVTDKQYLTGCDIKDLCLFIEEAKRQADGLSGVTLEYLARNY
                     GTAYRDVVGLAREKKSLSETLNEDGEIMAQVVYAVRDEMAQTLADIVMRRTGIGTLGN
                     PGDDVLRKVAQVAADELHWDQQRMEKEVEAVVNLLKIPVD"
     gene            complement(19600..20316)
                     /locus_tag="PLG98_01980"
     CDS             complement(19600..20316)
                     /locus_tag="PLG98_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOE32047.1"
                     /translation="MTRKVFARPVTRSRLHEEIVTVIQQQIMSGQIAPGEKLPTEREM
                     AENFNVNRATVREALRKLENIDLLEIRHGDGLYVKDYLNSGNFDLIKAAVKMDGGTEI
                     IFNILEARRYLVPQMAYLAAQRRTSSDVDGLKQILSEKNITMLEKDIRIHQQIARCTH
                     NLLCTIGLNFFNQIFRDYGHLYFNDPENVERSRQFHDEIFEAIRRKKADDARNIMRDV
                     LIYAEEAVKNKIKRPTFDKE"
     gene            complement(20503..21348)
                     /locus_tag="PLG98_01985"
     CDS             complement(20503..21348)
                     /locus_tag="PLG98_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE32048.1"
                     /translation="MPFFKRGNATIYFEDDGWGDPIIAVHGLIENTTYWKTTAEEIIK
                     KYRFIPMDMRGHGKTVVEGEFCGFDAETIGDDIEALADYLNIGEFHLLTHSTGGFAGV
                     RYAMRDSGRLASLILTDTGSSTSVIPGDVETIRKFNDKLACYFEKFNWEQIVANLWAN
                     PFPFFRGIVESPQARDLMRSAQDMISLGDRSDIARFVRSFYTDPDPKIEGLRGIRCPT
                     LIIYGEKDDLFIETSKMMAREIPHAQLLEYPGVGHMTALECPQRLAKDIIEFIKENSR
                     QTFDL"
     gene            21529..22470
                     /locus_tag="PLG98_01990"
     CDS             21529..22470
                     /locus_tag="PLG98_01990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE32049.1"
                     /translation="MYKKIFLAVLLVIVVSSCAPTHASRYREGSNFNQQTNLTIQDEQ
                     RLTREALPQILKDYPSAKNQELQKYISDLGMKIVRANHLEGNPYHYTFSVVDVADINA
                     FALPAGTIFVTVPLIALASNEAELAGVISHEIGHVVARHTAERMYVMEQAQNKSWMYA
                     TGGAVVGAIVGYGLGTVLCSDGDSACKARAALIGGALGAGGGLLAQKYTFLVNSREDE
                     MEADRIGFKYAVAAGYDKDQVGTFYEKLLEIEKKSGQSGNAIAKNLNDALSTHPPSEE
                     RVRQMRELAAASPVRDAVIHTSQFNRAKQIAKKIGKK"
     gene            22486..23136
                     /locus_tag="PLG98_01995"
     CDS             22486..23136
                     /locus_tag="PLG98_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOE32050.1"
                     /translation="MLTALLTIFVSSFIIALSGAMMPGPLLTVTISESSRHGFMTGPL
                     LIAGHALLEILLLAALLLGLAPFFQMPAVFVVTALAGSVILLWMAFNMFRSLPNVRLA
                     WDAHEKSGNHPVINGILMSVANPYWIIWWATIGLGYILYSRQFGLRGIGFFFAGHILA
                     DLSWYSFVSAAVTRGRHFLNDRLYRGILSLCAVFLVVFACSFLYAGIEKLSVILSG"
     gene            complement(23322..24014)
                     /locus_tag="PLG98_02000"
     CDS             complement(23322..24014)
                     /locus_tag="PLG98_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE32051.1"
                     /translation="MKKIGFLVVMLMLMAVFTTAQAAEVRAGGLPALAADDSNATSTV
                     NLSREGEVRAGGFPKMDKYDTCNDCTPPQCPPPPPPPPPVEEKVCPPCPECPACPPPP
                     VVEAPKPAIIEKGRQTLDVKFAFDKATIKPGYYKDIDALANVMKDYPDLNVVIEGHTD
                     SVGSDAYNKKLSQERADAVKKYMVDNAGINANRLKAMGYGEEKPIASNSTAEGRAQNR
                     RVEAAVDYVIEK"
     gene            complement(24158..25159)
                     /locus_tag="PLG98_02005"
     CDS             complement(24158..25159)
                     /locus_tag="PLG98_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE32052.1"
                     /translation="MRQYKCSVEVLPDVFCIRLPLPGRKPGPVNVYLFKGRKNTLIDT
                     GMAQTAGILKKALAEHHLGFADIDTIIITHGHPEHFGAARKIVLAGRAIVMAHEGDRT
                     FIEKGYGISAKQYKKFFRITGVPRNLEIILRLLFFFFKRMADPCRVDTIVREDDQIDI
                     GRYRAKILETPGHSRGSVSIYLEQEQTLFCGDTVIKHITPNAFVVPDEDATLPVRLSQ
                     DEFFKSLMKIKSLSPRIVYSAHGKPIDDVDKIIAGYEKAFAERQENVLALIHAGEKNI
                     YRIARRLFPNIGGWRLPLEIILSISEVYSAIQILQRKGKVCLRMRNNLLEVDQYRHP"
     gene            25380..26969
                     /locus_tag="PLG98_02010"
     CDS             25380..26969
                     /locus_tag="PLG98_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE32053.1"
                     /translation="MDNESLLSFGRRDFLKYALLAAQALTLPSLHGCAIPKKNVPAID
                     DATFLIKNANLVDVTSGLTVENSWLLVENGTIAAMGTGNIKNNYRGMVFDLQNRFLIP
                     GLIDAHCHATASPVFSMRMTDLPNHARQQKQNFVSAVESGITTIRDMGAFPVLLKRFI
                     RDIEKGNMPGPRVVYCNSILNVMGSHPEIPPSDINFFALPASLFIGMIMNNFKNTKEM
                     EECLEENARGASFIKLTLDNRTIFCKKNKAIPVYSKEQLDIIFNFAGKNGLPVVGHHH
                     FKFGFDRAMAYPFHSIEHLIADRILSDDDVLRMAKNNVAIVPTMTVAQSYLMEEAFDN
                     IPPPYATPDVMDELHVRKNYFGHEAFQHCDPVLHKQNLAALKYYKTIGRDNLWEHKKF
                     LVDPDVYFGMMMNGYANLKKMNQAGILIGCGIDAGMPFCYFGGNYREYEILQRNGFTN
                     IEILRCATISNAKILRLEDKIGSLETGKYADMVVLDKNPLEDIRALRSPCLVFKQGDP
                     MYSAINLQENGIMGRQPAKNT"
     gene            complement(27019..28287)
                     /locus_tag="PLG98_02015"
     CDS             complement(27019..28287)
                     /locus_tag="PLG98_02015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE32054.1"
                     /translation="MFDYMMTKEQLKIRDEVRDFIKWVPRQMVLDMDTDKIKFPKEFL
                     QEAARRNLMGCRYPKKWGGRDMDWVTTCMVMEEVGTIGYEMACVFGVGAELVCDAIIL
                     HGTDEQKEKYVKPLLRGDLFAAECLTEPRGGSDFFGATTKAEDMGDYYLLNGMKRFIV
                     GAEGADFFLVYAKTHPDAKPQESITCFIVDRTEGVEVKYLYGLMGCRGGGTGRIIFRN
                     VKVPKKNIVGKLQGAYAVFNTMMIPERLGTAAMTIGAARPALEIATNYTSRRKAFGQI
                     IAQFEGVSFQIAEATMLLDAARAMAYVTAKAVDAGADMHRIRRMVSQSKKFITESCQK
                     VVHNSMQVMGGIGYTNVFPIERIYRDIRLASIWTGTSEVMSMISAHEWYREYLMQKAG
                     QKTREHELDAEEAHEEEKIYDDEDMWKKGW"
     gene            28689..28964
                     /locus_tag="PLG98_02020"
     CDS             28689..28964
                     /locus_tag="PLG98_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32055.1"
                     /translation="MLHENEMIMIVIGLTLLVFLVLNYRQLKQTPFFQLLFVSYCLST
                     TGWMMTVLEGFFFEASLNFIEHLCYASSAVLFAVWCWMIPGRTRGNT"
     gene            28961..30982
                     /locus_tag="PLG98_02025"
     CDS             28961..30982
                     /locus_tag="PLG98_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32056.1"
                     /translation="MIHPVAFLDIIVILAALAAMAGLARRANDYFLISTRVLLACLSF
                     FLLLYGICLAVEWSGISSRFDRIEDYIGALLPMSWAFVFYSFLNELSFRNIQDLNTQL
                     KQRVDLLKEAELRFRTIFDSASDGILIADVREKTFFAANKKLCDMLGYTKEELQKLGL
                     PDIHPEASLALVNEQFDKLSAGEISVAQNIPLLKKDQDVFFSDVSASLMTFDKKKFLI
                     GIFRDITERRKAEEKLLKSEKKFSSIFHLNPNPLAITDVDTGTFVDINQAFVNWTGYS
                     REEIIGFSAHDLHLWVNLKDREKIVNIVTQTGEASSVEIMMRQKSGQIRTVLFSARFI
                     EIEQTRYLLTLAHDISERKQAEEQYRNIFENAQEGIYRATPEGRFILANRSMARILGY
                     DSQEDLITEITDMASQIYLEPQERTKLIQVMEQKGSIKNNEIQFRRKDDRPIWVSQTM
                     QAVRNEKGQIIYYEGIIEDITERKENVERLRRALGGTVKAIATLVETRDPYTAGHQRQ
                     VSDLARAIATEMGLPNDRIEGLRVAATIHDIGKVSIPAEILSKPSKLTNLEFDLIKTH
                     SQSGYDILQDIEFPWPVAQIVCEHHERINGSGYPNHLKGDEILLESRILSVADVVEAI
                     ASHRPYRASLGIDAALAEIEKNKGVLYDAGVVDACLRLFRQKDYQLTER"
     gene            complement(31145..31318)
                     /locus_tag="PLG98_02030"
     CDS             complement(31145..31318)
                     /locus_tag="PLG98_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32057.1"
                     /translation="MLKISKICFAVSGLLLIVDSTLMILNKPNPLGLPLPCPVTLTIL
                     GVGLILFSIAKIK"
     gene            complement(31467..32078)
                     /locus_tag="PLG98_02035"
     CDS             complement(31467..32078)
                     /locus_tag="PLG98_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOE32058.1"
                     /translation="MIYFDDLLQDDNICDAVSRIAGEFREKHNFPEVRQLGVVVPDVE
                     TAATQLEKRGIGPFFIASDTLKFWNERGEDRTFSGKLGMAIYKGYEVELLEPGIGSAF
                     YKSCVDGKGRMVLQHLGFLVNDVDDYRNKLEKAGCPTWIRGQIKSFPVVIDFVYMDSV
                     AQAGIILEFIDMRFLGIGVKPPGIIYHGLGKLEKITGIRCLKF"
     gene            complement(32193..33407)
                     /locus_tag="PLG98_02040"
     CDS             complement(32193..33407)
                     /locus_tag="PLG98_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOE32059.1"
                     /translation="MKALLLNLDPLRYVALHALRPLSKKFCYQGPFSTVKLVDIPEPV
                     LPSPEWVKIKTRLCGVCGSDINLMFMKDSPSAMPFTSFPCVPGHEFCGDVVDVGADVK
                     KIKKGDLITAIPALNCETRGIKPVCKSCASGTTANCDNFAEGAFAPGMFTGICKDING
                     GFAEYVIVHQSQARVVPAGVSPESAALTEPLAVGLQAVMDNRPADKDKVLVIGGGVIG
                     AMVVKSIRALDCTCDITVVEPSTFAADYIKQCGANRTMKGGIIEAAVKITKGRAYRPM
                     LGERMVMGGFDKVFDTVGNARTLNLSLRVTATNGTLSVIGIGQEVKLDLTTLWLKLQT
                     VKGCYGYRYNSVASSRKHTFDIALELIASRKIQVDDMLTHQLPLENYREMIEINLQKE
                     TRKAMKTAFCFM"
     gene            complement(33412..35079)
                     /locus_tag="PLG98_02045"
     CDS             complement(33412..35079)
                     /locus_tag="PLG98_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOE32060.1"
                     /translation="MAKIMGGHLVGKYLKEVENIDIVFGISGGHIEAVLDSFTEYKIR
                     TIDVRHEQAAAMMAHAWSVYTGKPGVCFLTAGPGFANGLTGIANAYLDNAPLVVLCGR
                     HPLRDDLKGALQEINQMDIVHPITKWAATCYDPKRIPEYLSIAFRYAVEGRPGPVFLE
                     LPPDILGVKIEESLAPMPRRTTRKYTVHPDDADLKAAAQIINNAKNPLFIGGSGVRFS
                     GKSDALKAFIEKTGIPFMLMNYGRGELPDSHSHSVMDMGSVGLMFGLPQTDVLIAAGL
                     RFNWLLMSGSLIPAATKVVRIDIDPHEIDRNRMADAGLVGDVGSVLEQLTPLADKKDH
                     SAWSETLKAAGRSLLDYELRIRETATVPIHPVRLVAQIQKVVGDDTVFVTDGGDTNYF
                     GLAFETRQKAGVIAAAAGLFGCLGTGIPFGMAAKLARPDKKVVVLNGDGSFGFNAMEF
                     DTMVRHNIPVVVVINNDCAWGMIKHSQELSIGADRLQCAELGVRHYEKVVEGLGGHGE
                     FVTKDEEIIPALERAFASGKPACVNVMTDPTVTSPATPLFYQALKME"
     gene            complement(35192..35551)
                     /locus_tag="PLG98_02050"
     CDS             complement(35192..35551)
                     /locus_tag="PLG98_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32061.1"
                     /translation="MRFSALLFILYQILKWAAKMKPVYRSYVGSIHQVRIMIKTADGK
                     RGRLFIFDRGTVSSKGGADHPYDSAIVWSDAATGFRVMASKSDEEQFKAAAAGKMKLD
                     GMAFFAQWFNDGVKLVM"
     gene            35689..36747
                     /locus_tag="PLG98_02055"
     CDS             35689..36747
                     /locus_tag="PLG98_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE32062.1"
                     /translation="MNQQSRADSIHLISSPTLPYFRSVNGIAILARYVEKYGIHVNTL
                     LTGSGLHPRDLDDPEMFVTPEQELAVMRKILFLVPDPKIGLIIGQQYHVGVQGKLGAA
                     AIFSDTGLEAITMFFKYIALTLTYFQYELHVKDKLAFVNVRELIDLKDLRVFVCEREF
                     VSIYRIVSDILKAPIPVNEIRFAYPKPSYASFYEGVFHCPVKFNAPEHRIIFDSTLLS
                     MPLPMSNPIARKTYEQECKQMCQRLKVKETITDQIRQEILFKEESVPSFTQLSRHLNL
                     SPRTLRRRLTDEKTSYKSLVADIRKKKALDWLKTSDCPLETIATRLGYSDISNFYRAF
                     KRWTGRNPGFYRKEHGRI"
     gene            complement(36871..38250)
                     /locus_tag="PLG98_02060"
     CDS             complement(36871..38250)
                     /locus_tag="PLG98_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE32063.1"
                     /translation="MKTRLDILITDDDRAHRVMLKKLLNGWGYNVTEADDGLVAVEKV
                     RKSSFDLILMDIRMTNVSGIEALEQIKQINADIPVIIMTAYASVETAVQTLKKGAYDY
                     LTKPLDFDELQVSIARATEHSRLKKENEYLKEKLGEKFDRRNMIGQSPSMIKLLEVVE
                     QVAATQATVLITGESGTGKEVIANAIHFNSARKNAPFVKINCAALTETLLESELFGHE
                     KGAFTGADKRREGKFVQADGGSIFLDEVSEMSPAMQVKLLRVLQERELTRVGGHDVLK
                     IDVRVIAASNKDLKKEIRDGRFREDLFYRLNVVTLDVPPLRERSEDIPLMAHAFLKMF
                     AENNAKVIKGFTPQAMQKLASYSWPGNVRELMNAVERAVVLCRTETIGEEDLIFTMAD
                     QALSREVLSAGQKMPAASGNRSLEEIEKQSILEALQSCRGNKSEAARRLGITRKTLRK
                     RLHKYEDGE"
     gene            complement(38455..40134)
                     /locus_tag="PLG98_02065"
     CDS             complement(38455..40134)
                     /locus_tag="PLG98_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32064.1"
                     /translation="MMQEKKKKIRWDISIWAAVSAITVLMVILGMMMFMQFQQSETQA
                     QALFIEKGATLIQSFEAGLQDRVDGKNDRFYFQKLLMATAEQPDIDYMIVTDSKGKIL
                     ADSDPSMLDQQYGIDLDTEKIARSKDMHWRQVTNPGGAGTFEIYRGLFPWRQTDAALH
                     HRSLIVFVGFNMDKIEKASQEDIYRTIIRAFILILIGSLAIVSLFLVQTYRLTRASLS
                     RVRVFAEALVRNLPIGLLAVDKDNRIIASNEHARDLFAGDPSNVERQDAGRILPGPLV
                     RLLTKVTMSSGLIEEDVVLSSETKGEQIWEVVVAAFADRDVPEGKILLARNVTAVRGL
                     EKEVAHSRHLNAIGSLAAGVAHEIRNPLSSIKGFAVYFKQRFAGNPDDEETADIMIAE
                     TERLNRVIGQLIEFARPLTLKTEKTALSGLVMQSVKLIEVEARKNGVQVDARVPEDLP
                     AVEIDPDKIKQVLLNIFLNALAAMPDGGRLSVGISQTQAGQEVLISDTGSGIEKNDLP
                     RIYDPYFTSKPAGTGLGLAVVQKIMEAHGAFLNIESQAGAGTKVTLRFAVS"
     gene            40452..41003
                     /locus_tag="PLG98_02070"
     CDS             40452..41003
                     /locus_tag="PLG98_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32065.1"
                     /translation="MIRKIINRRLLKNPYFDKAHHLLACLFYEGVEERIIGYLNPLWI
                     INDIGAEELTVLVKIKNGDMSDFLFSAIYFIQGLNIRYFFISPGGKFFVGSVPDKRLS
                     AFVGKNIFNDIFHGAVFRNDFFVKSNIPQIARVKFNPFGRAEYRACRMKEDHQREKHN
                     PTHCFDHDAPLKKENFFDIILTQ"
     gene            complement(41154..42452)
                     /locus_tag="PLG98_02075"
     CDS             complement(41154..42452)
                     /locus_tag="PLG98_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32066.1"
                     /translation="MKETGRNQASKEIKVLKNKIKELENLLNTTKVGQTRMSTGLVYR
                     TIFRMSPNTIVVSKLEDGTIYDVSDSFCEKSGFARKQVIGKRASTLGIWLDQDREEIK
                     KRLLRNGRYRNMEVRHQVKGGKMLQCLQSAELITIDDERYIITVVVDITERKMMEERL
                     RENEEKYRQLFDNSPAGIYQIDYQTGRFLKANDVFCKYVGCSQEEVTSLTAFDILTEE
                     SKKRFLERVERLSQNLEVPDSVEFEILNKKGESFYIQLQIRNVCDVKGRVVAADVVAH
                     DITERKQAEDELKSFAEDLEEANITLRVLMNQRDEDKQEIEEKLQTNMRDLVMPYLRK
                     LKRLNLDGRSKNYLDILESNLNEVLSPFMKNLQSSFNHLTPQEMQIISLIKKGKKTKE
                     ISEMLNASTKTIATHRNNLRKKLNLRNSKINLRSYLLSSG"
     gene            complement(42619..43203)
                     /locus_tag="PLG98_02080"
     CDS             complement(42619..43203)
                     /locus_tag="PLG98_02080"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOE32067.1"
                     /translation="MHIPPVFVYPEAVLFWTALIGSFYLEIKFAGALARNPSNTQDAG
                     TRLLIKIGYRVAFVLALMASLTPWFVITWQRPALAVGTGMLIAGSIFRQYSIRALGKY
                     FTPEVVVKADQPVIEEGPYRWIRHPAYSGAFIMFAGIGMALGNWLSFAVLFLENCFVY
                     SLRVKAEEKALIDTIGGPYRVYMTRTKRFIPFIF"
     gene            complement(43285..44181)
                     /locus_tag="PLG98_02085"
     CDS             complement(43285..44181)
                     /locus_tag="PLG98_02085"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOE32068.1"
                     /translation="MNDNLIFQETPDLINPYILIGYRGWLNAGEMATGSIDYLRRKLD
                     ARKFASIDIRNFYIWQVPGFDPAQVMRPHAVVERGLIKSLNEPANEFYFWKSGKENDL
                     ILFSGFEPNVAWPEFTSAVLNIAKRYQACRIYVLGGVFDQVPHTRETIVYAVLSRPEM
                     KHEFQSFPLLNYTGPCSFSTLLVNHAGREGIEAASIVARVPPYIETFNAKIGCEYLKK
                     VLSRTSLNIDLSDLKKSGDAVNKMMENDFRQNLEALVQLRQLEQMYDESLMQGYGENP
                     EVDIDELMQAMHEMKKDIPKKH"
     gene            44376..44645
                     /locus_tag="PLG98_02090"
     CDS             44376..44645
                     /locus_tag="PLG98_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE32069.1"
                     /translation="MVFLPEGRYYGSLYRSFLCREINNLLVTGRAISMDRGALASARV
                     MGPCLAEGQAAGTACALALSSGQSIDAVDTGKLREMLAKDGARLK"
     gene            44860..45318
                     /locus_tag="PLG98_02095"
     CDS             44860..45318
                     /locus_tag="PLG98_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOE32070.1"
                     /translation="MPAKKLKEFLDSHGVKYVKLTHSTAYTAHEIAQSLHISGRKVAK
                     TVIVMIDGKMAMAVLPGNRSVNFDHFQKAVGAKKIELATEAQFKGMFPDCELGAMPPF
                     GNLYGMEVYADQHLTDDDEIFFNACSHSELVEMSYPDYERLVKPNVVKLT"
     gene            45349..45594
                     /locus_tag="PLG98_02100"
     CDS             45349..45594
                     /locus_tag="PLG98_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32071.1"
                     /translation="MNGKYQEIQVECYSGFKANERPVAFTYQGERQEIQEVVDRWYHG
                     GLYSSKPVINFFKVRTADGKVYLLRYQFDSDAWSLRI"
     gene            complement(45636..47156)
                     /locus_tag="PLG98_02105"
     CDS             complement(45636..47156)
                     /locus_tag="PLG98_02105"
                     /inference="COORDINATES: protein motif:HMM:NF036848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOE32072.1"
                     /translation="MNSENIPVIIGAGQVTKREASSVQGGSPIDLMAAAVKIAAEDGG
                     VTEKYLQQADLLMTTSLFSDDGIANPPGCVADKLNLTDARCRVSGFGGVMPHAVLHHA
                     MQEIAGGKAGLVILTGAEAQHTMQQAKNSKTFTGWNVASGRPCLPPLTPLGLFDGACE
                     TEHIHGLSLPAYVYPMFENALRRRYGCTIEEHAEKIGRLMSRLSLVAAKNPLAWFQHS
                     FTPEEIMTLSETNRNTAFPYTKRMNAMLLVNQAAALLVASEAQAQKMGVNPLKWVYVH
                     GYAEANDHWNVLEREGYGFSPAIEIAGRTALKQAGLTIEDIDFFDLYSCFPVAVQVTR
                     DMLGIPENDSRDLTVTGGLPYFGGPGNNYVMHSMAQMVEVLRRHPGRTGLVTGNSFYM
                     TKHSTAVCSTRSPENNAAATADTRTCQQAVDKRPKYEIDPTPSGRATVDTYTVIYDRD
                     NLANKGIVIGKEENGKRFAAFTPSDPSLFSAMIEKDFCGVTGRVVSKDKINLFTPD"
     gene            complement(47171..47488)
                     /locus_tag="PLG98_02110"
     CDS             complement(47171..47488)
                     /locus_tag="PLG98_02110"
                     /inference="COORDINATES: protein motif:HMM:NF022815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HOE32073.1"
                     /translation="MKKATKESWTQKVKETSFALDLQSGVFTFDNPRRIAESLKESAE
                     SSTQRKAEPFQSAMSMLNYYINRAGTKLPAERKAILEQAKDELRVLFGRGNKSKTSLK
                     IKK"
     gene            complement(47555..49102)
                     /gene="putP"
                     /locus_tag="PLG98_02115"
     CDS             complement(47555..49102)
                     /gene="putP"
                     /locus_tag="PLG98_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HOE32074.1"
                     /translation="MTGNFNFYMLMSIAAYMVVVIVIGFVFARRNDQIGDFYLGGRKL
                     GTIVTAMSAEASDMSSWLLMGLPGVAYLSGIADAGWTAIGLAIGTYVNWLTVARRLRC
                     YSQLSQNAITLPEFFSNRYRDSKKILTWIASIVILIFFIPYTAAGFASCGKLFNSVFG
                     LDYTTAMLIGAVVVVAYTTVGGFLAASTTDLIQGIVMSIALLSILMFGAAVAGGIDAV
                     WQKADAIPGYLSMMFSHNSTTGQTERYGLITIISTMAWGLGYFGMPHILLRFMAIRDE
                     KKIVFSRQIAVTWVVVSLAVAVLIGIVGQTLSLNGEIAALEGSASETIIIRMAQLLSH
                     TGVISSLIAGVVLSGILAATMSTSDSQLLVASSSVTENLIKECFDIRLSEKTGMMIAR
                     SAVIVIAAIAAFIARDPDSSIFVIISFAWAGFGAAFGPVMLFALFWRRSNVYGAIAGM
                     VSGGVMVFVWKYLVRPLGGVWNIYELLPAFVFACLAIIVVSLLSKAPAREITDEFDAV
                     VKMGNRK"
     gene            49336..50004
                     /locus_tag="PLG98_02120"
     CDS             49336..50004
                     /locus_tag="PLG98_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOE32075.1"
                     /translation="MLDVIEKALKEGRKTLSEYESRQVIESAGVAVAVAALTKTKEEA
                     IREAEQMGYPVAMKGCSAELSHKTEAGMVALNVNSAQDVAQVFDALTGKVKNLDGVLV
                     EKMVRGSRELVIGLSQDSSFGPCVMFGLGGIFTEALKDVTFRVAPLTKQDALEMIDEI
                     KTKKLLGEFRGSPAVDRNALAKALVGIGDLAIKYEAIAEIDINPLIVCGDKPVAVDAL
                     VVLK"
     gene            50136..51527
                     /locus_tag="PLG98_02125"
     CDS             50136..51527
                     /locus_tag="PLG98_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOE32076.1"
                     /translation="MLDYFFNPKSVAIIGASSNMNKGGYHIIKNITSGFPGKIYPVNK
                     NYPDMLGLPCYKDLASIPGNIDLAIYFIPSKELPDTVNECAKKGVKGIIIESGGFNEA
                     GAEGARIQKLALDNAARAGIRLWGPNCMGFIDANKRYVFSFINSLVWPDVLRGGNVGM
                     IVQSGMLSAGFLLHALQEGIMGVSKVCSIGNKCDINENDILEHLIDDKDTEVIACYLE
                     SLVDGRKFLDLAKKTEKPIIVLMGGRSQEGAKAAQSHTASLSGNYKIASAAFRQAGII
                     EVFDPAELTDIARAFAKNMPFHPEKEKGTAILTFSGGAGTVTTDLMEDCGLKLAKLSD
                     RTLAAVAELFPPWNKPDHPLDLWIAIERHGFEKVFRNSLTAVINDPGVDSIIFHSYAS
                     PLIGQDFFEELAAMIKKNKKPAVLWIEGRKDFAEHIRGIAENAGLPAYREMNRCVTVM
                     NGMKRHFTKKPMS"
     gene            51540..52070
                     /locus_tag="PLG98_02130"
     CDS             51540..52070
                     /locus_tag="PLG98_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOE32077.1"
                     /translation="MARIPDFQPGPADTEKKFLQGKPPFDLNIFKIITHAPVGIAREF
                     IGLPASVLMAGKLDPVLREMAITRAGILCNSAYEVHQHRKLSKLVGLPEEKIKALDTG
                     SASPVFSGIEKLVLRFTEETVLQREVSDATFAEISRHFSEDLLVELSIAVCCYMAVSI
                     FLNTFEVNLEENDVPF"
     gene            complement(52544..53515)
                     /locus_tag="PLG98_02135"
     CDS             complement(52544..53515)
                     /locus_tag="PLG98_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32078.1"
                     /translation="MKICPVCQTRFKDKTQKCRYCNVVLEPLTPASASQLPQPDQQAK
                     QPKIVKDSRKKRQGKAPIERQTGFGKTWNRNKYPFIVVIILIVGILLAIVQKCSEKKV
                     EVSNTQAVEQQAVYAPVNQPAASDNRMIAAAPARDEANGLFRKAFELCISGKCTDPRK
                     AIEYLSEAIKLKPDYADAYNNRGNAYIEIGQYPEAVQDYNESIKLQPQSIYGYNNRGL
                     ALSKLGKHQEAIADYNEAIRLKPDSAIIHNNRGAAYLALGQYQKAVEDFSEAVKIKPD
                     YVDAYYNRANVYFDHMNKEDGCNDAQKACELGKCDLLEKVKGKGYCS"
     gene            complement(53845..>54320)
                     /locus_tag="PLG98_02140"
     CDS             complement(53845..>54320)
                     /locus_tag="PLG98_02140"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HOE32079.1"
                     /translation="FISGRHFMGRSDQRHQQTIAKTVMLIPDFSKYSLALFEGGALIH
                     ASNGKGVRPLFEALEKFRAKSGLILHDKIIGLAAAKLVVYSGIIAKVNTTVASAPADR
                     FLKDHGILMNAAGITANILTQDKRAVCPGEVIALKTKDPADFIRQIKTMLDNQRK"
BASE COUNT        13212 a        14150 c        12654 g        13804 t
ORIGIN      
        1 ccggggcggt caaaacgaac agcagcagga gcatgatccg atggggaatg aaatccagaa
       61 aaggaaacca gtgctgcatg gaaccgaaaa aaatgatgac acttaaaacg gctccacagg
      121 caactttcag tttcagttcc cggagttctt taagacgcga cgcctcgatg ggatcggcaa
      181 gatgattttt gatttcgcct aaatattcat acccctgttc ggcgacaacc ctggcaagcg
      241 ccgccgggcc ttcccattgc gtcgaatgca tgacggttgc atgtccgttg gcaagattga
      301 caaccgcgtc ttgcacaccg attacttctt tgatggcatt ttccacgcgc cggacgcagg
      361 cggcgcaggt catgccgccc acggcaacaa cggttttctt gagccccccg tccgcaggcg
      421 ttttattcaa caacgtcata ccccgctttt ttgatcgctg ccgcaacgac agtgggatcg
      481 acaggtttta tttcctcata aatcgccaca ccgtttttca aatccacttc aacattccgg
      541 acgccatcca cagcattcag ggccttcgtc accgccatga cgcaatgccc gcaactcatt
      601 cctttgattt ttatgctttt catttcatca ccctccctat attcatttta gtttaataat
      661 ttttttgata atagcaaggt gaaacagggt tctttcggga ctttcccaga aacgttttgt
      721 cctgatatcc tgcttgattt aaaatgtatt ctgtattatt gaaaccctta tagaaaaaaa
      781 ttatcctcaa tcgtgatcca cggggaaatt gataggcgac attttcatct ttttttacag
      841 gattaacaat gatcattcgc tgttggggtt ccagagggtc catccctgta tcgggcagac
      901 tgtatcagcg ctacggcggc aataccacct gcctggaaat caggacgaaa gacaacaaca
      961 tattgattgt cgatgcaggc tccggaatcc gcgaggcagg caacgccctg ctggcggagg
     1021 gccgtttgaa tttcacgctt ctgctgacgc acgcccactg ggatcacatc atgggatttc
     1081 ccttcttcaa acccatctat tcctctaaaa cccgaatgga catccgggga tgtcccttcg
     1141 cccagacctc gataaaaaaa atgctttccc ggattatggc agctcctaat tttccggtca
     1201 attacagtca cattcgtgca aagattgtat accacgaaac ctgtttcaaa cccgtctatc
     1261 ggcttggcga tatgctcatc acccccattg ccctgagtca tcccaacggc gggaccggtt
     1321 acaaattcga ggaagacggc aaaacgttca tttttctcac agacaatgaa ctgggccaca
     1381 gacatcccgg aggactcccg ttcaggcaat atctggcatt ttgccggaat gccgatctgc
     1441 tcttccatga cgcggaatat aaagaggcag aatacaaaaa aacccgcggc tggggacatt
     1501 cttcctataa agatgtcctg aaactgtcgc ttggggcccg cgtgaaaaaa ctgggactgt
     1561 ttcatcacaa tcaggagagg accgaccccg atgtcgatgc gattctgaag gactgccgcg
     1621 acaccgtcaa aaaagaaggc gggaaaatga actgtgtggc cgtcggccaa tccatgcagt
     1681 ttacgcttta atttttcaac gcgttttata aacaagatat catgcttttt ttattacaca
     1741 ttgtcctgat ggtaattgcg acactgggca tcatggccgg agtgagtgcc gccatgtttt
     1801 tccgcaaaaa gaaaaactgg ctgaaaatcc ataaaaccct caacacgttg agcctggcgg
     1861 ggatcactgc gggaatcatc atggcatttt tctatgttgc cggcaccggc ggaaaacatc
     1921 tcaacgatgt tcatcagatg accgggctgg cagcgtttac cgttgtcctc atcgccatag
     1981 ctctgggaca ttaccagttc aaagccctca acaaatcggc cattcgggcg gcgcaccgct
     2041 ggatgggacg cgtttcgatt ttcatcctgg cagccgccgc ctttctggga ttgaggctga
     2101 tcaatcttct ctaaccggcc aatgccgcaa tcgtgagccg tttttcgtta tgtttcttcc
     2161 cacaacccgc gacgaattaa aaaaattgaa atgggacaaa ccggatatca ttctggtgac
     2221 cggtgacacc tacatcgaca gtccttttat cggcgtgtcc gtcatcggcc gcgttctggt
     2281 cgatgccggc tttcgtgtag gcattatcgc tcagcccgac tggcatacgc cggacgatat
     2341 cagccgactg ggcgagcccc ggcttttctg gggaatcacc ggcggctgca tggattccat
     2401 ggtcgccaac tacacggcga cgaagaaaaa aaggaaaaac gatgatctga ccgcaggcgg
     2461 aagaaacaac cgtcgtcccg accgcgccgt tgtggtgtac gccaatctga tccgccggta
     2521 ttttaaagag accaagccgc tggtactcgg cggcgtcgaa gcctccctgc gtcgcatcgc
     2581 ccattacgat ttctggtcgg attccatccg ccggtcgatt ttgttcgatg cccgtgccga
     2641 tgtgctggtc tacggcatgg cggaaaaagc cgtcgtcgaa ctcgcgcagg ccctgcgcga
     2701 cgggaaagac acaacagata tccggggcat ctgttatctt gcgcccgccc ccccgccgca
     2761 aaacgcactg gaactgcccg cttatgagga tgtgcttgcg gacaagaaaa catttatcag
     2821 gatgtttaac aggttctatt gcaacagcga tccgctgaca gcgaagcgcc tgtatcaaaa
     2881 gcacggcgac cgttatcttg tgcaaaatcc tccgcagccc catctgacaa cgccggaact
     2941 ggatcatatt tacggacttg atttcacgcg cgacgttcat cccttctaca gcgcccgggg
     3001 caatgtcagg gcgatagaaa ccattacgtt ttccataacg tctcatcgcg gttgctacgg
     3061 tgaatgtaat ttctgctcca tcggtctgca tgaaggacgc accgttatca gccgttcaga
     3121 aaaatccatt ctggatgagg cgcaaaaact ggccggacgt tccgatttta aaggctacat
     3181 tgcggatgtc ggcggatcga cggccaacat gtacggcatc gaatgcgcga aaaaaatcaa
     3241 gaaaggggcc tgcaacggca aacgatgtct gtccccgagg gtatgcgacg ccttgccggc
     3301 ggatcacagc cggcagatca atctattgag aaatctgcga aatgtcagag gcgtcaaaaa
     3361 agttttcgtg gcctccggca tccgatacga tctgctttta aacgacaggg cacactgcct
     3421 ggcgtacatg aaagaactcg tcgagcatca cacctccggt cagttaaagg tcgcgcccga
     3481 acatagcgaa tggccggttc tgaagctcat gggcaagccg caggcgcagg ccctgctgaa
     3541 tttcaaacat ctttttgaaa aaacatccgc cgtttcaaaa cgcaaacagt ttctcacata
     3601 ttatttcatt gccgctcatc cgggatgcac ggaagaggat atgagacgtc tcaaagcgtt
     3661 caccaccaga gagctgaaaa cgaatccccg gcaggtacag atcttcaccc cgcttccctc
     3721 gacctattcg gccctgatgt actttaccgg tatcgatcct tcgaccggca aaaaaatctt
     3781 tatcgaaaag aacatggaga aaaaggaaaa acagaaaaac atcctgatcg ggaataagag
     3841 gtcataaccc cgaaagcgca ccggttttgc cggcactcag cggcaacgcc cctcttgttg
     3901 aacagcggtt aacaaatcac agtcccccag tgcacaggcc ctgtgtgcat ccggacaacc
     3961 ccgaacatcg tttcgctgcc tgaaataacc atacgccctg ttatggtagg ccagggtgaa
     4021 atccggtttt cgtctgatcg cttcgtcata atcgctgatc gcctgctcat accggccgag
     4081 atgaaagtaa gcaagtcccc ggtcatgaaa ggccctggca tggttttgat ccagggcgat
     4141 ggtccggttg aaatcgtcaa tcgcctgttg ataccggcct gtttttgaat acaccattcc
     4201 cctcatataa tgcgcatcga cctcgtcggg gtggagacga agggctgtct gataatcctt
     4261 cagggctccg tcataattcc gggtggccgc ataaacattt ccccggttga cataggctct
     4321 ggcgtaaccg ggattgagca aaaccgcctg attaaattgc gcgatggctt cttggctttt
     4381 tccttcaaaa taaaaggcat gacccagata ttcgtgcaaa acgtaaacat ctttttgaac
     4441 cttcacggcg tgttccagca gcgtgacgct gttctgccag aatccgcatt gacgccatga
     4501 ccccaaagcc agcattgcca gaaaaaggac cgccgccggg aacataattt ttttccgata
     4561 gctttcttct ttgaagaaaa gagggatccc ccaggcggtc atgagggcaa tgccgaccga
     4621 cggcagataa tggtagcgat ccgccatggc atcgctggat atctgaacga tcttgatcac
     4681 cggaaaaatg gttacggcat accacagcca tcccgcaagc agatagggat gtcgtcttct
     4741 catccgcacg acggcaacgc tgacaaccgc aatgaaaatt accgatccgg caagtcgcag
     4801 accgggaatc gagtccggaa aaggatagaa aattgaaagc gtgacgggcc aaaacgtttt
     4861 gatcagatac gccgcaaacg aaacgacggc attggccagg cgcagatcaa gaggatactg
     4921 tttgatgtcc gacgctttca gatcgtaaag cgttatgatg acaacaagca aggataacag
     4981 gaagaaggga gatttttctt ttatttgccg caacaggttt ttctgtgccg acacaaaacg
     5041 cctgagcggc cagtaatcca gcagcagcag cacgaccggc agggtaacga ccatcggctt
     5101 gctcatcagc gcgcatacaa aaccaagcac gaccggccaa tatctttgga tgaccggttt
     5161 ttccgtataa acgacataaa ggcagagggt cagcatccag aaaaaggcgc tgagaacatc
     5221 tttccgttcg gaaatccagg caacggattc cacatgcaga ggatgcaacg cgaaaaaagc
     5281 cgcgatgaag gcgctcttcc agagggcgcc ggtcatacgg tgaaaaagcc agaagagcat
     5341 cagcgaactc atcatgtgca gcaaaaggtt ggtcagatgg taaccgccgg cgttcaaacc
     5401 gaagacccga taatccagca tgaaggaaag ccagacaaga ggatgccata aatcggcata
     5461 tttcgtactg acagcccaga tgaagctgtc cggcgtcatt cctgttgtga tatgccggtt
     5521 gaaaatgaca tacgccggat cgtcaatgat aacaaactca tatcgaatca gctgcgaaaa
     5581 aacagccaga actgctatgg ccagcagggc ataaatcatt ccagcctgtt tgtccggagg
     5641 aatattgatt ttattcggca tgatcgcttt ttaacatatg tgaagccgga gttcagcaat
     5701 tttctggacg aaagtttagc tcgccggatg attgcctgaa gccgatccgt tcatcttttc
     5761 gccgactttc ctatttgcca ttgactttcc ctgaacattt ttcctataat ctttcatcgt
     5821 caatctccta tcttcagact ggattatctt taaagaggca ccatgatttc cagacgcgat
     5881 tttttaaaaa ccctctccgg actggccgtg accgtcggcg gcacatggat gatactgtcc
     5941 gatccgtcct ggcagagact gtttcaggaa gggcatgcgg gccttgcctt cggcgatnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg atcgccttca cctacaacga
     6121 accgacggtt ttcaccgaat atctcacaga catcgcccgc aacgcccggc aacagggcat
     6181 ccgctgcgtc atgatcagtt gcggtttcat gaacgaggcg ccgcttgcgg aaatgtgcga
     6241 aacgctggcc gccattaaaa tcgatctgaa aggctacagc gaggattttt accgttcggc
     6301 ctgccaggcg gaattgaagc ccgttctaag aagcatcagg cagatcgcca aaagccgcgc
     6361 tcatctggag attgtaaacc tggtcgtccc cacattgaat gattcggaca agatgctcaa
     6421 aggattgatc gactgggtga tgggagaagt cggccccgat gtgccgttgc atttttcccg
     6481 ttttcatccc gattaccagt tgcgcaatct tccgcccacg cccgttgcca cgcttgaacg
     6541 agcctatcgt atggcgcgtg acagaggatt gcattacccc tatgtcggca atgtccccga
     6601 tcatccggga aacaacacct actgtccgaa atgcggaaag atcgtggtta aaagacaggg
     6661 gttttttatt accgggatga atctccgcaa cgggctgtgt acattctgca agaataaaat
     6721 tgccggcgtt tggacctgaa aggagaacct accatatgaa atggctgcgt ttgctttgtt
     6781 ttttgccggt gattgttttt tttctgacgt cggcctgtca ggccaacaaa gacgccgctg
     6841 ccggcgtccg ggaaccggct tttgccggca agttctatcc ggcatccgca gacaaactgc
     6901 gtctgaccat cgaaaagtat ctgcaagacg ccgtgttgcc caaggtcaaa aaaccggtgg
     6961 ccgtcattgt gccccatgcc ggatatatct tctccggcca gatctgcgct gacggtttcc
     7021 gacaggccgg tcatcacccg tacgatgtcg tggttatcct gggaaccaat cacaccagcc
     7081 cgggcctgca gggagtttcc ctctatcccg gcgaaggatt tcgcacgcct ctcggcattg
     7141 cggcagtgga caaaaccgtc attgcgtccc tgctcacgga atctcctgtc gattgcatgc
     7201 tggacaaatc cgcgcatgtc tctgaacatt ccatcgaggt gatcgtgcct tttgtccaga
     7261 ttgtttttcc caacgccaaa atcgttcctg cggttgtcgg gacgcaggac aggcacacca
     7321 gcatccgctt cggtcaggcg ctggcaaaag ttttaaaaaa caaaaatgca ttaattgttg
     7381 ccagctccga tctgtcgcac tatccctcgc gggataatgc cgtcaaggtt gaccgccgga
     7441 cgcttgatgc ggttgccaca ctcgatcctg ccgtcttcca aaaaacgata caggacacga
     7501 tgagcaaaaa cattccgaat ctctccacgt gtgcctgcgg cgaagcgccg attatcgcgg
     7561 cactggctgc cgcccgatta ctcggcgccg atcgcgggaa cgtcatcagt tatgccaatt
     7621 ccggcgatgt ctccgtcggc gattccgctc aggtggtcgg ttacggcgcg gtcgtactga
     7681 cggccgggca aaaggggatt tcttcttctc aaacggatct gcagaaaacc gcggctgtcc
     7741 ccttcgatct gggcacagcc gataaaaaag cattgctggc ctttgcccgg gaaaccctat
     7801 cccgtttttt tacgaccgac acggttcctc tggcacggca tttcccagcg catctgaatc
     7861 acccccgcgg cgtctttgtt acgctcaaaa aaaacggcgc cttgcggggc tgcatcggcc
     7921 atttaataac ggacgaaccg ctggccaagc tcgtgggcgc gatggcgctg caggcggcat
     7981 ttaatgacgg acggttccct cccttaaccg ccgaggagct cccggatgtc gacatcgaga
     8041 tatccgtgct caccccgatg aaacaggcgg cccgaacctc cgacattatg gtcggccggg
     8101 atggtgttgt gttgagcaaa aacggacgtt ctgctgtttt ccttccccaa gtcgcactgg
     8161 aacagggctg gagccgcgat gacatgctgg atcatttatg cagcaaggcc gggctggaaa
     8221 aaagatgctg gcgtcaggac gcaacatttt caatttttca ggcgatcgtg ttcagcgaat
     8281 cgcagctgaa ataaacgcat gaccgtttat cttttatcca tcggactgat ttccatcctg
     8341 gggcaggtta tcctgctcag ggaattgaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnt atccatcgga ctgatttcca tcctggggca ggttatcctg ctcagggaat
     8521 tgaatgtctc tttctacggt gtcgaactga tctatctgtt cgccttgggc atctggctgt
     8581 tctggaccgc cgtcggcgtg atcgccggac gacggatggc tcttccgtcc aggcatagcc
     8641 cggccgtttt gttcatcctg ttcgggatga tcctgttcct ggacgtcgcc ttcatccgtt
     8701 ccagccgtct gatcttcggc ggtgtaccgg gcgcctatct cgattttttt caacagctcc
     8761 ttgttgcgac cctgtcgatt ctgcccgtgg gacttttatc cggactttta tttcagcaga
     8821 ccgccggatt gtatgtagac tccggccgga cgctggcagc cgcctacgcg gtggaaagcg
     8881 ccggcggttt gatcggcggg ttattctcca cgctgttgat tatcttggac acccagaatc
     8941 ttttcgccgc gctgatctgc tcgatcgtat cgtttgcgac gcctcttctg ggtgctggaa
     9001 agtccctggg agcttttttg cggcggacgg caatcgtgct gcttgcaatg tttgcgggcc
     9061 tgattttcaa tgcatcacct ttgcatgaca tctcgacccg ttggaatcat ccgaatcttc
     9121 tggacagcag cgactcgcct tacggcagaa tcaccgtcac cagaatgttc gatcagattt
     9181 ccgtattcga aaacgacgcc ctgtcctttg aaacggaagg aaccgatgcc gaatatttct
     9241 gccatctggt aatgcttcag catccccatc ctcgaaaggt tctgattctg ggcggcggga
     9301 tggaaggcat catcggggaa gccctaaaat acggaccgga ccatattgat tatgtggaaa
     9361 ttaatcccgt tatgacgaag cttgttatga agcacctgcc ggaggccatc cgtcagtcgc
     9421 tccagtcacc cgaagtgcgc atcatttatg ccgatccgag gcggtatctc acaaacagcg
     9481 ggacgtatga tctcatcctg gtcggcatgc cggaaccgtc atccggacaa gccaaccgtt
     9541 tttatacaca ggaattcttt aaacaatgcg cggaaaaact taaatccggg ggccttctcg
     9601 gatttaaatt gcaaacggct gaaaatctct ggacgggacc gctgatcggc cgcaatgcaa
     9661 gtatctatca tgccctgcgt tcggtttttc ccgctgtgct ttttctgccg ggcacgacaa
     9721 acatcgtcac cgcatcgttt gcgccgctgc ccgatacacc ggacatcatg accggccgcc
     9781 tgcagcatct ccaattagag acccggctga tttcagcccc ttacatccgc taccttttta
     9841 ccaacgacag atttgccgcc gtcaaaaatc tcctgaacga aaaaaaggac ttgctcaatt
     9901 ccgatatcag accggtttgt tatcaatacg cgctgatcat ctggctgtcg aaatttttcc
     9961 ctcgcgcgac gctttttgat ttctcctcga tcatggagaa gaccttgttg cgatatctgc
    10021 cggtctgttt cttgatggtc ttgcctgtcc tctttctggc cagccgtttc cggcccggac
    10081 tgcgccgcaa aatactcgtg gctgcggccg gttttgcagg catcgcgctg gagacgatcc
    10141 tgctgcttta ttttcaactc aaatatggcg tcatttatcg ggatatcggt ttgttgctga
    10201 tgagttttat ggcgggcctt gcgctgggcg ctttggcggt tgataaactg atggcgcgtt
    10261 ttgcggacca tcgcctggga acccggcggt gcggcattat acttgtggcc ggatcctgct
    10321 gcctttgcgc gataaccgcc ctcttaatca gaaacagtct gcctgccggt ctctggctga
    10381 ccggtctcct gctgactgca acaggtttta ttgtttccgg gatctttgcc tatgccagtc
    10441 tttatgagat tgatgatcag cgcgcaatca tttcaccttt gtatgcggcc gatctgatcg
    10501 gcggatgttt ggggtctgtg cttgtcagtc tgattttcat tccgctggcg ggcatggata
    10561 ccacgatgtg gggaatgctt ctactaagcg tctatgcctt cctgctgttg tagccgccgg
    10621 cgcttcaacg ctcaatcctt ggcatacggc gtccccgaag caagccatgg atttttttca
    10681 gaccatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcaa tccttggcat
    10801 acggcgtccc cgaagcaagc catggatttt tttcagacca tggcggctga tcgtctttac
    10861 tgaaaagatc gttccccata accagacaat ccatgtccgt ctgcatgaag caccggtagg
    10921 cgtcttccgg cgtgcagacc atcggttcgc cgcgaacgtt gaagctcgtg ttgacgagta
    10981 ggccccaacc gcttttttgc ttatacgcct tgatcagctt ccagaaccgt tcgtttgttc
    11041 ttttgctgac gctttggatt ctggcggaat agtcgatgtg ggtcaccgcc gggagatcgg
    11101 atcgcatgtg ataaagcctt tcgtacaggg tcatctgccg ctcattttcc ggttgcggcc
    11161 tgcggcgatg ttccttcagc ccggcaacca gcatcatgta aggcgacggt cggtcgagat
    11221 cgaaatattc ggccacgtct tcttcaagga cggccggagc gaacggcctg aattcttcgc
    11281 ggtattttat tttcagattc agtttttttt gtatgtccgg caggcaggga ttcgccagaa
    11341 tactgcgatt gcccaaggcc cgcggaccat attccatgcg tccctggaac cagccgacca
    11401 ccagcccccg gaggatccgt tccgcgacaa ccgcacataa ttcatcgaaa tcttcgtagt
    11461 gtacaaacga tgcctggtat gttttggcag tgcgcttgat gtcgtccgat ccgaattcag
    11521 gacccaggaa agcgcccgcc atggcatcct gttccgacac gctttttctc ggctgttcca
    11581 tcccgacatg ccaagcggca aaagccgcac ccagcgcacc gccggcatcg ccggccgacg
    11641 gctgtatcca gagatcgtca aatattttct ggcgcagcaa cgcgccgttg gctacgcaat
    11701 taagcgccac cccgccggcc ataaccagat aattactgcc ggttaatttt cgcgcggtgc
    11761 gtgccatccg ggagacaaca tcttcagtga tctgttgtat cgccagcgcc atattcatgt
    11821 aagactgtac gatctcactt tcccgttgtc ggcggggaat tccgaacagt ttttcccatt
    11881 tttcatcccg gcacatggtc agaccggtgg caaagtcgaa gtaatccata ttcagaagaa
    11941 cggatccatc ctcccggata tccaccaggt ggtccagaat ttttcttcgg tataaagcgg
    12001 tttgcccgga gtcggcatga ccgtacgggg ccagccccat gagtttatac tcgccgctgt
    12061 tgactttaaa cccgcaataa ccggtgaagg cggaataaag caatccgacc gaatggggaa
    12121 aatcaagctc ccgaagaatt ttgatggacc ggccctcccc cagaccgatg gtggctgtcg
    12181 cccactcgcc gacgccgtcg atcgtcagaa tggctgcctg atcaaaaggg gacggataaa
    12241 aggcgctcgc ggcgtgagaa agatgatgtt ccggaaaaag cagtttgacc cggcccggac
    12301 cgatgccggc gataccgtcg tgcagcattt tacgcataaa aattttttct ttcatccaga
    12361 cgggcattgc ggccataaaa cttttcagtc cccggggggc aaatgcatga taggtttcca
    12421 gaagcctttc cagtttcaga tagggttttt cgtaaaaggc cacaccgtca agatccgcca
    12481 ggtgacgtcc gccctcccga aggcagtatg ttgccgcatg gacgggaaag acgctggtat
    12541 gtttttcacg ggtaaagcgt tcctcgtgag cggcggccac gatccggccg tcaaccaaaa
    12601 gcgcagcggc gctgtcatga taatacgccg atatcccgag aatggcaggc aacatgtctt
    12661 cctcttaaaa aagtgtataa atgaaaggcc ccacggctga tccgcccgca aagatcatca
    12721 aggctccgca taaaagcagc atcagaatga tcggcagaag ccagaatttt ttcctgactt
    12781 tcaaaaaacc ccatacatct ctgaaaaaat ccatgtttgt ctcccgtttc agtacggatg
    12841 tttgatatca tccgccctga attcgtgctc gcgtattttg aaaaccgaat gttgattttt
    12901 tttccattct ttcaactgca gcgtatcgtt gccgaggcct cgccggacaa gaccgacggg
    12961 caacacaaaa atcaaaaaaa tcaggaacaa gatcagccgg gacatcacag cgccgaggat
    13021 gtgcgcaatg cccagccaga ccatcgccca cggcttgaat atccggggcc aaacgatatt
    13081 cagaaaaagc agcagcagag caaaaccgta aaaaaacgtt atatccgaaa aaaaacctgc
    13141 tatcagacaa agcagcgcgg cagccatgcc ggcatctttg gcctgatcgg ttgtgattgc
    13201 gtcgggaata aatttcattt tgtctttgta atcttttctg aaacgtactg tgattttaat
    13261 atcacaagtc ggtcaggaaa accactctca tcatcgtggc aaaggttatt tcctcaccgg
    13321 agactgccgc aaggggcgat gatccggaat ggacatccga aagaatgttg ttggtctgtt
    13381 ctgaaaataa ttcattccgg catccccgcc accggttcgc cggatgatgt cagaccgggc
    13441 aacgatcggt ttgattttgc aaagacggcg gcaaatgcct gcttagattt ccttgatggt
    13501 tcttaatttt ttgcacccgc cgtccagcca caggcagaga tcctgtacgg tcacattgct
    13561 tgaacattta aattgccggg ccccttccgg caagaattcc cggtcctgca tgctttggta
    13621 gatttggagc gaaccgtctt cgaacagcac tataaaaaat gacatgtttc acctttcgat
    13681 tgcagaacga caaacatttc ttactgtgcc ttcggacagg ggcctatata tttgcctttg
    13741 atcttgttgg acatcgtcat ggccggttgt cctttcattt taaaactggt ggtcgacgcg
    13801 ccgttcatgg ccttgtccgt ataggttgtc gttccggacg tctgaacgac cccttccttg
    13861 cccgtacatt ccacctgata gctgacagtg ccgccgctga ccttttggct gatgatctta
    13921 cattcaaagt ttttgtcttt attctgggga acgggattgc ttttcgtgat gcactgacga
    13981 aacgtcgtgg gcggcatgga ttgcggcatg cccttcattt ccacctgggn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gacgaaacgt cgtgggcggc atggattgcg
    14161 gcatgccctt catttccacc tgggtggtga tttcccaaag cccttccttc agttcggcaa
    14221 aaacgacggt cgtccataaa agacaaacaa tcatcacaaa gaaaaacaat gcttttttca
    14281 taacgcaaat ctccttgatt tctgatttca gccgatgatc atccattcgc ctgttggatt
    14341 caccggccga tcaaacacgc aacgactgcc tgttgacgag tgattatact tttacttccg
    14401 gtgaatcgca atctatttgt tcccgctgtt taatttcccg ccggataacc ggtaatatct
    14461 ctgatgtctc tgaacaacgg cttgagccgt tcgtacatct tcagatacac ccgcctgtaa
    14521 agattgtcgt acagaccgtg attatcctta tccggttcaa aaaccctgcc ggtccgggtc
    14581 atgttggtaa ccgcagacgc aaaatcggaa taaagcttaa gcccgaccgc cgcgtcaatg
    14641 gccgcaccga gtgcggatgt ttcaaacgtg tgaggacgtt ccgccgcgat gccgaaaatg
    14701 tccgccgtaa tctgcatggc cgcgtcgctt tgcgaaccgc ctccggaaac tttcagccgg
    14761 gtaaccggta ttttcgtctt ccgctgggtc aattccccgc cctctctcaa tccgtaaacc
    14821 agcccctcca gaagagcccg gtaaaggtag gcgcggctgt gcacatcgcc gaaaccgata
    14881 attgatccct ttgcaaaagc gtccacttcc cggccaggcg tccagtaagg ctggagcatc
    14941 agccccttgc atcccgccgg tacatcctga atcagggaat caaagaattt ctccggaggt
    15001 tccgctgtct tctcggcaag catacactcc tgaaggccga actcttcctt gaaccagctg
    15061 accatccaga atccccggaa gatcgacacc tcggtgtaat acgtatccgg aacggaagcg
    15121 ggaaacggcg ggatcatcgg atgaagctca acatatttgg gtgtggccac gtcaaacgtg
    15181 gcaatggttc cgaaactcag acatccggtt tccggcgtga gacaaccgga accgaggatc
    15241 tcgcagccct tgtccgatcc ggccgcaaac accggcagcc cctcgggaat gccggtctct
    15301 gcggaagctt ttttcgtaat cgcacccatc atttcggacg gtttgaccag gcgaggaagc
    15361 ttttcttttt ccaccttgaa gatccgccac ttaaaatcga acttgcccgc ccactggtag
    15421 gtcttattgt caaacggcag ataaccgaca ttgttgcccg tcgaatcgac aaattcgccg
    15481 gtgagccgat gcgtaaaaaa tccggacagc accagatatt tatgcgtttt ttcccagatg
    15541 tccggctggt gctgacagat ccagttcgcc tcgcattcct gaatgacgtt ctccagcgta
    15601 tccagaaggt tgactgtttt cagcagcgga cgaagcggtg ccggcaggat ttttctggag
    15661 tcggcctttc gctggtccag ccagataatc gccggcctca gcgggttgcc gttctgatca
    15721 acattgacca tcgtggcgcg ctgcgtcgtc agcgtgacgc ctttgacatg ttttttcaga
    15781 acttcctggt cctgcaacaa ctgatgggtt gttttgcaaa acacatccca gtagtagtcg
    15841 ggccgctgtt cggcccagcc gggatgttcg gaaaaataag gttgaatggc cgttttgacg
    15901 atcgccttga tatttccccg gacatcgata agagcggccc ggatcgactg ggtccccgca
    15961 tccacggaaa gaatcaggtc ctgcgttgta tttttttcac ccgacatagt tatctctcct
    16021 ttttttatga ttcccgcaaa tcatcgcgct gctttcatca atccagtccc agctgaccgc
    16081 ccggattcat gatgttgtgc ggatcaaaat gttttttgag cgtccgcaga acgtccaact
    16141 gttctttgcc cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggatc
    16261 aaaatgtttt ttgagcgtcc gcagaacgtc caactgttct ttgcccagat gtttctccat
    16321 ccacggtccg atcatccgtc ccacgccgtg atggtgactc agcgaaccgc cgtgtttctg
    16381 aatgttgtcg ataatacccc gctggaattt ccgaaactct tcgggatctt tcatatgcgc
    16441 aataaagata aaatagagat tggtcccctg cgggtaaaag tgagaagcgt gtgtcatgca
    16501 catggtctgc ggcctgttct tgatatacgc gcgcacgccc tgatgcagac ggtggagatt
    16561 gtcccaggtc acggcggctt ccagcgtatc gatcaggatg tcgtaatcgc caaggtcctc
    16621 gcgcatatac ggttcggtat accgggtgtg ctcccatttt tttgccgcgt aaccggtcag
    16681 cgatatcgcc ccgtgttttt tcgcaactgt tttgattttg cgcaccacaa gcttcgtata
    16741 atcgcaatcg ccctcggcat ttcccagact cagacagcgc tgcatgggct tgaatccctt
    16801 catcacgagg attttatcca caaaatccgg catgccgtag agtttcagcc cccggtcggt
    16861 ctcctccgga tcggaaatcc ggtaaattgc cggcttgccg aattccgact gcatgatttc
    16921 ccggctggca ttcaccgcgg cctcccaagt cggatacata aaactgaaac gccggcgatt
    16981 ctccggcatg tagcggaata ttttcatggt gacttccacc agaataccga atgatccttc
    17041 agaccccttc atgatgtcat tcaccttggg accggttgcg gtcgccggat aatccagcgt
    17101 tttaaacgtc ccggcgggtg tgacatattc ctgactgaaa acaatatcat aggcgtcacc
    17161 gtaataggtg gaggcctgtc cggaccccag tgtgacaacc cagccgccca cggtggaata
    17221 ttcgaaggat tgcggaaaat gtccgcaggt gtaacgatgc tttgttttaa aaagttcggg
    17281 cgctttattg agaccctctt cataagccgg accgaacatc cccggctgaa cgcgcgcagt
    17341 ctgattgaac tcattgacct ccagcagttt attcatgtgg gtgctgacca ccagggaaac
    17401 gccgcccctg acgggacgac aaccgaagtt tacggaagaa ccggcgctgt aaaccgttat
    17461 gggaatgttt tcctgatgac agtattgaac aattttctgc acatcctgtt tgtcacgcgg
    17521 atgcaccacc acgtccgcga cttcccggat aatccccaaa cgcagttcca gcatctcttc
    17581 cgtggttttg cctgatgcgt atttcacacg gctataatca tcgagtgcaa cattttcgcc
    17641 gcccacaata ttccggaggc aatccatctg ttgcggtttg agtgcctggg cacggtttaa
    17701 cgaaaccggt tcaagtcctt cttttactct tgaagcgaag tccttgtcgt ttaaatgaaa
    17761 ttcttccttg atcatcctgt accaggcatc gctgggatgt ttgaaactgt gcagatctcc
    17821 gtatttaaaa atggatctgt acgttccggc tgccggtgcc ttttccgtcc agtttggccg
    17881 gaaataatga gatatcatca ccatgtcctc cttttctctt gaactctttt catgttcatt
    17941 caatttttta atcaacaggt atcttcagca gattgacgac cgcttccact tccttttcca
    18001 ttctctgctg gtcccagtgc aattcatcag cggcaacctg tgcaacttta cgcaacacat
    18061 cgtcaccggg attgcccagt gtgccgatac ctgtacggcg catgacaata tcggccaggg
    18121 tttgggccat ttcatcacga acggcataca cgacctgcgc cataatttcg ccgtcctcgt
    18181 ttaatgtctc ggacagactt tttttctcac gcgcaagccc gacgacatcc cggtacgccg
    18241 taccgtaatt gcgcgccaga tattcgaggg tgactccact caatccgtca gcctgacgct
    18301 tcgcttcctc aataaaaaga cacagatctt tgatatcgca gcctgtcaga tattgtttgt
    18361 ccgtaacact ccggccaaga tttcttccca tttttttggc gacgatcgcc agacagtttt
    18421 cggccagttt gcggctggtg gtgtatttac cgccttccac ggtaatcagt ccgtccaatc
    18481 cgtcatcttt attatcgtag atttcatatt tgcgggaaga agaataggtt tcgctggttt
    18541 ctttttcaat aagaggacgc agcccgccgt aggtatattt gacatccgca tagctcagtt
    18601 tgccgtcgcc gaaggagctg ttcacgtcag cgatcagttc catgatgctt tctttggtaa
    18661 cgcggtagtc gtccggatta ccgttaaacg gtttgtccgt cgtgccgatc agtgcgtgat
    18721 tgcgccacgg aatcaaaaag aaatgacgtc cctccggcgt catcgacccg attgtatagt
    18781 tgccggtcag cagtttctta gacgtgatga tatgaatccc ttcggaccgc ctaagcgttg
    18841 cgttgacctg accatccggc ttggccatgc ccaggacgat atccgcccag ggtccgccgc
    18901 agttgatcgt tacggttgcg ggcacattgt gcatcttacc gctgaacaaa tctttcacct
    18961 ttacaccgag aacatgcttt gcgccgtcca tgagaaaacc ttccactctc gcgtaattgg
    19021 ctgccctggc accgtgggcg acggcggatt tgataaaggc caacgtcagc ctttccggga
    19081 aaagactggt gcagtcgtaa aaaatagaag cgccggtcaa tccttcagcg cggacgtgag
    19141 gttcatcctg gagaacttgc cgggccgaaa tcgttctgtg gacgggaatt tttttgcagg
    19201 gatcccatgt gaaatttttg tcgtaggaca gaacgtcata gacgagcatc cccactttca
    19261 ccacccactt attatttttc aacggctttt tgtaatgcgc catcatcatc ggccacggat
    19321 agacaaaatt cggagcaata ttctcaagaa cacgccgttc gcgcagcgac tcgcggacca
    19381 gtccgacttc accgtttacc agatatcgca aaccgccgtg aatcattttg gatgtcaccg
    19441 ccgatgtcgc ccaggaaaaa tcattttttt ccaggagagc gactttcagc ccccgcgtgg
    19501 ctgcttcata agcgaccgac gcaccgctga tgcctccgcc gattacagca acgtcaaatt
    19561 tctcacctgt gtaattttca ataaatcttt tcatgaccat cactccttat caaacgttgg
    19621 tcttttaatt ttatttttca ccgcttcctc cgcgtaaata agaacgtctc tcatgatgtt
    19681 gcgggcgtca tcagcctttt tcctcctgat ggcttcaaat atttcatcgt gaaattgccg
    19741 ggaacgttcg acattttccg gatcgttaaa ataaagatgt ccgtaatcgc ggaagatctg
    19801 attgaaaaaa ttgagcccga tggtacacag cagattatgc gtgcagcggg cgatctgctg
    19861 atgtatccgg atatcttttt ccagcatggt tatatttttt tcagaaagga tctgcttcag
    19921 gccgtcgacg tccgatgagg tgcgcctttg cgccgccaga taagccatct gcggaaccag
    19981 ataacggcgc gcctccagaa tattgaagat aatttccgtc cctccgtcca tctttacagc
    20041 ggccttgatt aaatcgaaat taccgctgtt gagataatct ttgacataga gcccgtcgcc
    20101 atgacggatt tccagaagat cgatattctc cagttttctc aatgcttcgc gcacggtggc
    20161 ccgattgaca ttgaaatttt ccgccatctc tctttccgtg ggcaactttt ctcccggcgc
    20221 gatctgaccg gacatgatct gctgctggat gacggtgaca atttcttcgt gaagacgtga
    20281 acgcgtaaca ggacgggcaa aaacctttcg ggtcattatg gttgtctctc ctagctttcg
    20341 gccttattgg ccgggtggtt cgaccaataa cacatcacag attctttgtc aatcattttt
    20401 ttgctataac cttaaaaatt gaggcttcag ttgcccttcc gttaatcccg gattggccgg
    20461 cagatggtat cgacggcaaa aaaatgctct tgtcaagaca gattacaaat caaatgtttg
    20521 tcggctgttt tctttgatga attcgataat gtcctttgcc agacgttgag gacattccag
    20581 cgccgtcata tgtccgactc cgggatactc cagcagttgc gcatggggaa tctcccgggc
    20641 catcattttg ctggtctcta taaatagatc atccttttca ccgtaaatga tcagggtcgg
    20701 gcaacgaatt ccgcgcaacc cttctatctt cggatcagga tccgtgtaaa atgatctgac
    20761 gaaccgggcg atgtcggacc ggtcacccag actgatcatg tcctgagctg accgcataag
    20821 atcgcgggcc tgcggtgatt cgacaatccc tcgaaaaaaa ggaaacggat tcgcccacag
    20881 attcgcgaca atctgctccc agttgaactt ttcaaaataa caggccagtt tatcattgaa
    20941 ctttctgatc gtttcgacat caccgggtat cacggacgtc gaggaccccg tatccgtaag
    21001 aatcagactg gccagcctgc cggaatcgcg catggcataa cgcacgccgg caaaaccacc
    21061 cgtggaatgc gtcaggagat gaaattcccc gatattcaga taatcggcca gcgcctcaat
    21121 gtcgtctccg atcgtttccg catcaaaacc gcagaattct ccttcaacga ccgtcttccc
    21181 gtgaccccgc atatccatag ggataaaacg atattttttg ataatctcct ccgccgtcgt
    21241 tttccagtag gtggtgtttt ctatgagtcc gtgaacggcg attatcgggt ctccccatcc
    21301 atcatcttca aaataaattg ttgcattgcc gcgcttaaaa aaaggcatgt ccgtgtctcc
    21361 ataattttcg tcatttcatc agaagtataa aacatttaaa acaaattttt ctcctggtca
    21421 atgcattata tccgcccgat cgcaaccttg acagaacgta tccggcatgg ttatctaaga
    21481 tcagagcgga tgatcagaga atatcattac cgcccgggga gatataaaat gtataaaaaa
    21541 atatttttgg ccgtcctgtt ggtcattgtg gtttcgtcct gcgcaccgac ccatgcctcg
    21601 cgctatcgtg aaggctccaa tttcaatcaa caaacaaatt taaccataca agatgaacaa
    21661 cgcctgacac gggaagcgct tccccagatt ttgaaagatt atccgtcagc caagaatcag
    21721 gaactccaaa aatatatctc ggatctggga atgaaaattg tccgtgccaa ccatctggaa
    21781 ggcaatccct atcattacac gttcagcgtc gttgatgtgg ccgatatcaa cgcttttgca
    21841 ctgcccgcgg gaacgatctt cgtcaccgtt cctctgattg cccttgcctc gaatgaagcg
    21901 gaacttgccg gcgtgatctc gcatgaaatc ggtcatgtcg ttgcccggca taccgccgag
    21961 agaatgtacg tcatggaaca ggcccagaat aaatcctgga tgtatgcgac cggcggcgcc
    22021 gtcgtgggcg ccattgtcgg ttacggactg ggcaccgtac tttgcagcga tggcgattct
    22081 gcctgcaagg cgcgagccgc cctgatcggc ggcgctctgg gggccggcgg cggactgctt
    22141 gcgcagaaat acacgtttct ggtcaattca cgagaagatg aaatggaggc cgaccgtatc
    22201 ggtttcaaat atgccgtagc agccggatat gacaaagatc aggtgggaac attttatgaa
    22261 aaacttctgg agatagaaaa aaaatccggc caaagcggaa atgccatcgc aaaaaatctt
    22321 aacgatgctc tgagcacgca tcctccaagc gaagagagag tccgtcaaat gcgcgaactg
    22381 gctgccgcaa gtcccgtcag agatgccgtt atccatacgt cgcaatttaa cagggcaaag
    22441 cagatcgcca aaaaaatcgg taaaaaataa aaccatgcaa agcgtatgtt gactgccctt
    22501 ctcaccatct ttgtttcatc ctttattatc gccttatccg gcgcgatgat gccgggaccg
    22561 cttttaacgg tgaccatcag cgaaagctcc cggcacgggt tcatgaccgg ccccctgctt
    22621 attgccggcc atgcccttct ggaaatactg ctgctggccg ccctgctgct ggggctggcg
    22681 ccgttcttcc agatgcccgc tgttttcgtc gtcacggcgc tggccggctc ggttatcctt
    22741 ttatggatgg cgtttaacat gttccgctct ctgcctaacg tgcgccttgc atgggatgct
    22801 catgaaaaat ccgggaatca tcccgttata aacggcatcc tgatgagcgt ggccaatcct
    22861 tactggatta tctggtgggc gactatcggg ctgggataca ttctgtattc ccggcaattt
    22921 ggtttacggg gaatcggttt tttctttgcc ggacatattc tggcggattt gagctggtat
    22981 tcttttgtct ctgccgcagt cacccgcggc agacattttt taaacgaccg cctgtatcgg
    23041 ggcattctgt ctttgtgcgc tgtttttctt gtggtgtttg cctgttcttt tttatacgcg
    23101 ggaattgaaa agctaagcgt gatcctttcc ggataatctt ccgtctgccc gcagaaaaac
    23161 caggcgcccg caacagctca tttcatcttc tccgcgcttg gattttcttt catgctcttc
    23221 agagcgcgtc aacggatatt ttgcactccc ccaccgaaca cataaaaaag ccctgcagga
    23281 aactgcaggg cttttgatcc aatgcatgga aaactgcttt tctatttctc gatcacatag
    23341 tcaaccgctg cttcaacacg acggttctgt gcacggcctt ccgcggtgct gttggaagca
    23401 atcggttttt cctcgccgta tcccatcgct ttgaggcgat tggcattaat tccggcgttg
    23461 tcgaccatgt attttttgac ggcatcagct ctttcctgag aaagtttctt gttgtatgcg
    23521 tcgctgccga cactgtcggt atggccttca atcacaacgt tgaggtccgg ataatctttc
    23581 atgacattag ccaaggcatc aatgtcctta tagtaaccgg gtttgatcgt ggctttgtca
    23641 aaagcaaact tcacatccag ggtctgtctg cctttttcga taattgcagg tttgggcgct
    23701 tcaacgaccg gaggaggagg acatgcaggg cattccgggc acggaggaca tactttctct
    23761 tctaccggag gaggaggggg aggaggaggc ggacactgag gaggagtaca atcgttgcat
    23821 gtatcatatt tatccatctt ggggaaacca ccggctctga cttctccttc ccgtgacaga
    23881 ttcacggtgc tggttgcatt actatcgtct gcagccaaag cgggtaaacc accggctctg
    23941 acttctgccg cctgagccgt cgtgaagaca gccatcaaca tcagcataac tactaaaaat
    24001 ccaattttct tcataatgtt ctcctttcgc actgcaatta taataatttt tttaaatgat
    24061 ctcttttaat attctattgc atatcaatta tttgggtaat ttatactaaa ctcaatattt
    24121 tatgtcaata taatatttat taaatttttc cggctcatta tggatggcgg tattgatcca
    24181 cttccaaaag attgtttctc attctcagac agacttttcc ttttctttga agaatctgaa
    24241 tggcgctata aacctctgaa atagaaagaa ttatttccag cggtagccgc cacccgccga
    24301 tgttcgggaa aagacgtctc gcaatgcggt aaatattttt ttctcctgca tgaattaagg
    24361 ccaaaacatt ttcctggcgt tcagcaaaag ctttttcata tccggcgatg attttatcca
    24421 catcgtcaat gggcttcccg tgagcggaat aaacgatccg cggtgaaagg cttttgatct
    24481 tcataagcga tttgaaaaat tcatcctggc tcaaacggac cggcagcgtt gcgtcttcat
    24541 ccggaacgac aaaagcattc ggcgtgatgt gcttgatcac cgtatcgccg cagaaaagcg
    24601 tttgttcctg ttcaagataa atactgacgg atcccctgga atgaccgggc gtttccagaa
    24661 tttttgcccg gtatcggccg atgtcgattt gatcgtcttc gcgcacaatg gtatcaaccc
    24721 gacagggatc tgccatccgt ttgaaaaaga aaaacagcag cctcaaaata atttccaggt
    24781 tgcgtggaac acccgtgatg cggaaaaatt ttttatactg ctttgcggat ataccgtatc
    24841 ctttttcgat gaacgttctg tctccctcat gcgccataac gattgctcgg ccggcaagga
    24901 caatttttct tgcagccccg aaatgttccg gatgtccgtg tgtaatgatg atggtatcaa
    24961 tatcggcaaa accaaggtga tgctccgcca gcgctttttt cagaatgcct gccgtttgag
    25021 ccatgcccgt atcgatcagg gtatttttac gtcccttgaa aagatagaca ttgacgggtc
    25081 ccggcttacg accgggcaga ggaagtctga tgcagaagac atccggcaaa acctcaacgc
    25141 tgcatttgta ttgtcgcatc agactcctta ctttttatac aatgatcttt ccgacaggcg
    25201 attgcacaaa acacgatagg gtcatttttt tgaaagtata agaagaacca tgcagcctgt
    25261 caaccaaaga tagtgtatgc aacttcctgc cggagaaaag aaccttttaa tatatcttga
    25321 cacgaaaaac ccgccgatta taattgcttc aatattattg gttgtcacaa cggtcacgca
    25381 tggataacga atcgcttcta tcatttgggc gcagggattt tctcaaatat gccctgctgg
    25441 cggcacaggc cctcaccctg ccctctctgc atggttgcgc catacccaaa aaaaatgtgc
    25501 cggccattga tgatgcaact tttctcatca aaaacgccaa cctggtggat gtgacgtcag
    25561 gactgaccgt tgaaaactcg tggctccttg tggaaaatgg aaccatcgcc gcgatgggaa
    25621 ccggaaacat caaaaataat tatcgcggga tggtttttga tcttcaaaac aggtttctca
    25681 tcccggggct gatcgacgcg cattgccatg ccacagcctc tcccgtcttt tcaatgcgca
    25741 tgacggacct tccgaaccat gcccgccagc aaaagcagaa tttcgtttcc gccgtcgaat
    25801 cgggcatcac gaccatccgg gacatgggcg ctttcccggt tctgctgaag aggttcatcc
    25861 gggatattga aaaaggaaac atgcccggtc cccgcgttgt gtactgcaat tccattctga
    25921 acgtcatggg atcgcatcct gaaattccgc cttcggacat taattttttc gccctgccgg
    25981 cttcgctctt catcggcatg atcatgaata acttcaaaaa caccaaagaa atggaagaat
    26041 gtcttgaaga aaatgcccgg ggcgcttcat tcatcaaact gacgctggat aatcggacaa
    26101 ttttttgtaa aaagaacaag gccatccccg tgtacagcaa ggagcaactc gatattatct
    26161 ttaactttgc ggggaaaaac ggccttcccg tggtcggaca ccatcatttc aaattcggct
    26221 tcgatcgcgc gatggcctat ccttttcact ccatcgaaca tctgattgcc gacaggattc
    26281 tttcggatga cgacgtattg agaatggcaa aaaataatgt ggccattgtt cccaccatga
    26341 cggttgccca atcctatctc atggaagaag ctttcgacaa cattccgcca ccgtatgcaa
    26401 caccggacgt catggacgaa ttacatgtga gaaaaaatta ttttggccat gaagcattcc
    26461 agcactgcga tccggtgctg cacaaacaga atctcgcagc cttgaaatat tataaaacga
    26521 tcggcaggga caacctctgg gagcacaaga aattcctcgt cgatcccgac gtctatttcg
    26581 gcatgatgat gaacggctac gccaatctga agaaaatgaa tcaggcggga attctcatcg
    26641 gctgcggcat tgatgcggga atgcctttct gctatttcgg cggcaattac cgggaatatg
    26701 aaattctgca gcgcaacggg tttaccaata tcgaaatcct ccgctgcgcc accatcagca
    26761 atgcgaaaat tcttcgtctg gaagataaaa tcggctcgct ggaaaccggg aaatacgcgg
    26821 acatggtggt tctggacaaa aaccctttgg aagatatccg cgcgttaaga tcgccctgcc
    26881 tggttttcaa gcagggcgat ccgatgtatt cagcgatcaa tcttcaggaa aacgggatca
    26941 tgggccgtca accggcgaaa aatacctgat acaggagcga tcaacaaaac aggatttcgt
    27001 catccggcgg cggacgtttt accatccctt tttccacata tcctcatcat cgtatatctt
    27061 ttcttcttca tgggcttctt cggcgtccag ttcatgctcg cgagttttct gtccggcctt
    27121 ctgcatcaga tattcccggt accactcatg cgcgctgatc atggacatca cctcactggt
    27181 gcccgtccag atcgacgcca gccgtatatc gcggtaaatg cgttcgatgg gaaacacgtt
    27241 ggtgtacccg attcccccca tgacctgcat ggagttatga accactttct gacaggattc
    27301 ggtgataaat ttcttgcttt gtgaaaccat gcgccggatt ctgtgcatat ccgcacccgc
    27361 atccaccgcc ttcgcggtga catacgccat cgcccgcgca gcatccagaa gcatggtcgc
    27421 ttcggcaatc tgaaagctga cgccctcgaa ctgcgcaata atctggccga acgccttgcg
    27481 tcgtgacgta taattggttg caatttccag agcgggacgg gcggcgccga tggtcatggc
    27541 ggccgtgccg agcctttccg gaatcatcat tgtattgaaa acagcatacg cgccctgtaa
    27601 tttccccaca atgtttttct tgggcacttt tacattgcga aagatgatcc gcccggttcc
    27661 gccgccgcgg caccccatca ggccgtagag atatttgact tccacgcctt ccgtccggtc
    27721 tacgataaaa caggtgatcg actcctgcgg tttggcgtcc ggatgcgttt tagcatacac
    27781 caggaaaaaa tccgctcctt cagcgccgac aataaatcgc ttcatcccgt tgagcaggta
    27841 ataatcgccc atatcttccg ctttcgtcgt tgccccgaag aaatctgaac cgccgcgcgg
    27901 ctctgtcaga cactcggcgg caaatagatc gcctctcaaa agcggtttga cgtatttttc
    27961 tttctgctca tccgtgccat gtagaataat cgcgtcgcag accagttccg cgcccacgcc
    28021 gaagacacag gccatttcat agccgatcgt ccccacctct tccataacca tgcaggtggt
    28081 tacccagtcc atatcccggc cgccccattt tttcggataa cggcacccca tgagatttcg
    28141 ccgtgcggct tcctgaagaa actctttagg gaatttaatc ttgtccgtat ccatatccag
    28201 aaccatctgg cgcggaaccc acttgataaa gtcccgcacc tcgtccctga tctttaactg
    28261 ttccttggtc atcatataat caaacatgcc attctccttt cctgttgatc attgcgcaga
    28321 gatacttttt acggattata acagaaatca ccttgaaacc aaaagacaca atgctaaaaa
    28381 atcttaccgt ctttttttgc cgtcttcatg cttcccgatg gcttgccgat atgtaaactt
    28441 catcatgatt cttagtcata ccggttttca cagcgccctt ctcggtcata atcctcttga
    28501 cacaacggat ggtgtttttt ataattcata tcatgggatg atcattctta tccacgtgtt
    28561 cccatgcccg gtatcgaacc gggcaacaac gtcataataa attcaggggc ctcaaaaaag
    28621 atgtaataga atcttcaata cgataactat ccaaaaagca taagcatttc tgaaaaaggg
    28681 aatcaaagat gctccacgaa aatgaaatga tcatgatagt tatcggcttg acgcttctgg
    28741 ttttccttgt cctgaattat cgccaactca aacaaacgcc tttctttcaa ctgctctttg
    28801 tcagttactg tttaagtacg acaggctgga tgatgaccgt cctggaagga tttttctttg
    28861 aagcatctct caattttatc gagcacttat gctatgcctc cagcgctgta cttttcgccg
    28921 tttggtgctg gatgatccca ggcagaacaa ggggaaacac atgatccacc cggtggcatt
    28981 tctggatatt attgtgatcc tggcggcatt ggcggccatg gccggattgg ccagacgagc
    29041 caatgattat tttctgatca gcacccgggt gttactggca tgcctttctt tttttctgct
    29101 gctgtacggg atttgtctgg cggttgaatg gagcggaatt tcttcccggt ttgacagaat
    29161 tgaagactac atcggcgccc tgctcccgat gtcgtgggct tttgtctttt attccttttt
    29221 aaatgaactc agtttcagaa atatccagga cctcaacact cagttgaagc aacgagtcga
    29281 tttgcttaag gaagccgaat tgcgattcag aaccattttc gattcggcgt ccgacggaat
    29341 tcttatcgcc gatgtccgtg aaaaaacctt ttttgccgcc aataaaaaac tttgtgacat
    29401 gctgggctat acgaaagaag aattgcagaa actgggcctt cccgacattc atccggaagc
    29461 atcccttgcc ttggtcaatg aacaatttga caaattgtcg gcaggagaaa tttccgttgc
    29521 tcaaaacatt ccactgttga agaaagatca ggacgtcttc ttctcggatg tcagcgcatc
    29581 actcatgacg ttcgataaaa aaaaatttct tatcggcatt ttcagagata tcaccgaacg
    29641 aaggaaagcg gaggaaaagc tcctcaaatc cgaaaaaaaa ttttcttcca ttttccatct
    29701 caaccccaac cctctggcca ttaccgatgt cgacaccgga acctttgtcg atatcaatca
    29761 agccttcgtc aactggacgg gatactcccg tgaagaaatc atcggttttt ccgctcacga
    29821 ccttcattta tgggtcaatt tgaaagaccg cgagaaaatc gtcaacattg tgacgcaaac
    29881 cggagaagcc agcagcgtag aaattatgat gagacagaag agcggtcaga tccggactgt
    29941 gcttttttcc gcccggttca tagaaatcga acagacgcgc taccttctga cgcttgccca
    30001 tgacattagc gaacgcaagc aggcagagga acaatatcga aacatctttg aaaatgcaca
    30061 ggaaggcatt tatcgagcga cacccgaagg acgattcatt ttggccaatc gctccatggc
    30121 aagaatcctc ggatacgact cccaggaaga tcttataacc gagataaccg atatggcctc
    30181 tcaaatctac ctggaacctc aggaacgcac aaaacttatc caggtaatgg agcagaaagg
    30241 ctccattaaa aataacgaga tccagtttcg cagaaaagac gaccgcccca tctgggtgtc
    30301 ccagacaatg caggctgttc gcaatgaaaa aggacagatt atctattacg aggggattat
    30361 cgaggatatc acagagcgca aagaaaacgt tgaacgcctg agaagagctc tcggaggaac
    30421 ggtcaaagcc atagccaccc tggtggaaac aagagatccc tatacggcgg gccatcagcg
    30481 ccaggtgagt gatcttgccc gcgcgatcgc cacagaaatg gggttaccga atgatcggat
    30541 tgaaggattg cgtgtggcag ctaccatcca tgacatagga aaagtttcca ttccggctga
    30601 aattctgagc aagccgagca aactgaccaa tctggaattc gatcttatca aaacacattc
    30661 tcaatccgga tatgacattt tacaagacat cgaatttccc tggcccgttg cacaaatcgt
    30721 ttgtgaacac catgaaagaa tcaacgggtc cggttatccc aaccacctga aaggcgatga
    30781 aattctgctg gaatcacgca ttttgtccgt ggcggacgtt gtcgaagcca ttgcctcgca
    30841 tcgtccctat cgcgcctctc tgggtattga tgcggcactt gcggaaattg aaaaaaacaa
    30901 aggcgttctt tatgatgccg gcgttgtaga cgcctgcctc agattattcc ggcagaaaga
    30961 ttatcagctg acggaacgtt aaaaaaaatt atcccaacga tctcaaatcg cgatgttcct
    31021 atccggaaac cgtctgctgc aggaaacatt aagcatacta tttcgacggc gcattttggt
    31081 ccaagccaga cagatcgcaa ccaatacggt tatttttcaa tacatggtcg aacagaggaa
    31141 gcgattattt tattttagca atggaaaaca aaatcaatcc cacacccaga atcgtcaggg
    31201 tcaccggaca cggaagcgga agcccgaggg gattgggttt gtttaaaatc attaaggttg
    31261 aatccacgat cagaagaagt cccgatacgg caaaacatat tttggatatt ttcaacataa
    31321 cgacctcctt ttcatccata ttattttaaa ttttccgccg gatagcatgc ggctaacgtt
    31381 catgggatag aatttaaccg gcgctttgat taccgtcgtc ttttttatcg ccggacaaac
    31441 aatccgggat gccgtcatgg gttctttcag aatttcaggc atcttatgcc ggtgattttt
    31501 tccagttttc cgaggccgtg gtagattatt ccgggaggct tgacgccgat acccagaaac
    31561 cgcatatcaa tgaattccag gataatgccc gcctgcgcaa ccgaatccat atagacaaaa
    31621 tcgatgacca cgggaaagga tttaatctgc cctctgatcc atgtcggaca accggccttc
    31681 tccaatttgt ttctgtaatc atccacatca tttaccagaa agcccagatg ctgcaggacc
    31741 atccggcctt ttccatccac acatgattta tagaaggccg atccgatacc cggctccaga
    31801 agttcgacct catatccttt atatatggcc atgccgagtt tgccgctgaa cgttctgtct
    31861 tccccgcgtt cgttccagaa tttgagtgta tccgaagcaa taaagaaagg cccgatgccc
    31921 cgtttctcca gttgcgtcgc ggcggtttcg acatccggga caaccactcc caactgacga
    31981 acttccggaa aattgtgttt ttcacgaaac tccccggcaa tccgggatac ggcgtcacaa
    32041 atattatcgt cttgtaacaa atcgtcaaaa tatatcatgt ctcactcccc tttattcaat
    32101 gagacaggtc aaaattttca agccttcatt acatgtgctt ttcaggacac tccttgcctc
    32161 gcggcgccgg gcaataacct gcttacggct ttttacataa aacagaaagc ggttttcatg
    32221 gccttgcggg tttctttttg gagattgatc tcaatcattt cccggtagtt ttccaacggc
    32281 aattggtgcg tgagcatgtc atccacctga attttccggc tggcgatcag ctcaagggcg
    32341 atgtcgaacg tgtgtttcct gcttgaagcg acagaattgt agcgataacc gtaacagcct
    32401 ttgacggtct gcagtttgag ccacaatgtc gtgagatcca gtttgacctc ctggccgatg
    32461 ccgataacgg aaagcgtgcc gttggttgcc gtgacgcgaa gcgacagatt cagtgtgcgc
    32521 gcattgccca ccgtatcgaa aaccttgtca aagccgccca ttaccatgcg ttcacccagc
    32581 atgggtctgt aggctctgcc tttcgtaatt ttcacagcgg cctcaataat cccgcctttc
    32641 atcgtccgat tggcgccgca ttgctttata taatccgccg caaacgttga cggctcgaca
    32701 accgtaatgt cgcaggtgca gtccagcgcc cggatgcttt tgaccaccat cgcgccaatg
    32761 accccgccgc cgataaccag caccttgtct ttgtctgccg gcctgttgtc cattacagcc
    32821 tgcaacccca ccgccagcgg ttccgtaagc gctgccgatt ccggcgacac gcccgcaggc
    32881 acaacacggg cctgactttg atgcacaata acatattcgg caaatccgcc gttgatgtct
    32941 ttacaaatac cggtaaacat accgggcgcg aacgcgccct cggcaaaatt atcgcaattg
    33001 gccgttgtcc cggatgcgca gcttttgcac accggtttga ttccgcgtgt ttcgcagttc
    33061 agcgcgggaa tggccgtgat cagatctccc ttttttattt ttttcacatc cgcgccgaca
    33121 tcaacgacat ctccacagaa ttcatggccc ggcacgcaag gaaacgaggt aaacggcatt
    33181 gccgacggag agtctttcat gaacatgaga ttgatatcgc ttccgcacac gccgcagagc
    33241 cgggttttga tcttcaccca ttccggagag ggaagaaccg gttccggaat atcaaccagc
    33301 ttcaccgtcg aaaacggtcc ctgatagcag aattttttgc tcagggggcg aagcgcatgc
    33361 aacgccacat accgcaaggg atcaagattc agcaacagcg ctttcatatg tttactccat
    33421 cttgagcgcc tgataaaaca acggtgttgc cggactcgtc accgtcgggt ccgtcatcac
    33481 gttcacgcac gccggcttgc cggaggcaaa cgcacgctcc agcgccggaa tgatttcttc
    33541 gtctttcgtg acaaattcgc cgtgtccgcc gaggccttcc actactttct cgtaatgccg
    33601 gacgcccagc tccgcgcact gaaggcggtc ggcgccgatg gagagttcct ggctgtgctt
    33661 gatcataccc caggcgcagt cgttgttgat gaccaccact acggggatgt tatggcgcac
    33721 catcgtgtcg aattccatgg cgttgaaacc gaacgaaccg tcgccgttga gcaccaccac
    33781 ttttttatcc ggccgcgcca gtttggccgc catgccgaag ggaatgcccg ttcccagaca
    33841 tccgaaaagc cccgcggcgg cggcgatgac gcccgccttc tgccgggtct cgaaagcaag
    33901 gccgaaataa ttcgtatcgc ctccgtccgt aacaaagact gtgtcgtcgc ccacgacttt
    33961 ctgaatctgc gccaccagac gcaccggatg aatcgggact gtcgccgtct cccggatccg
    34021 gagttcgtaa tccagaagcg agcggccggc ggctttcagc gtttcggacc acgcgctgtg
    34081 atctttttta tccgccagcg gcgtcagctg ttcgagcacc gatccgacat cgcccaccag
    34141 ccccgcgtcc gccatgcggt tacggtcgat ttcgtgcgga tcgatatcga ttcgcactac
    34201 ttttgtcgcg gccggaatga ggctgcccga catcaaaagc cagttgaacc tcagtcccgc
    34261 ggcaatcagc acatccgtct ggggaaggcc gaacatcaga ccgacgctgc ccatgtccat
    34321 caccgaatgg gagtgactgt ccggcaattc tccgcgtccg taattcatca gcataaaggg
    34381 aattcccgtt ttttcgataa acgctttgag cgcgtcggat tttccgctga agcgcacgcc
    34441 gctgccgccg ataaacagag gattttttgc attgttgatg atctgtgcag cggctttcaa
    34501 atcggcgtcg tccggatgca ccgtgtattt gcgggtggta cgccggggca tcggcgccag
    34561 cgattcttcg atcttaacac ccagaatatc cggcggcagc tccagaaata ccggaccggg
    34621 gcgtccctcc accgcgtagc ggaaggcaat ggaaagatat tcgggaatcc gtttgggatc
    34681 ataacaggtg gccgcccatt tggtgatggg atgcacgatg tccatctgat tgatttcctg
    34741 cagggcccct ttcaaatcgt cgcgcagcgg atgcctgccg cataaaacca ccagcggcgc
    34801 gttatccaga taagcattgg caatgccggt caggccgttg gcaaatcccg gtcccgcagt
    34861 gagaaaacat acgccgggct ttccggtgta aaccgaccag gcatgcgcca tcatggcggc
    34921 ggcctgttca tggcgaacat cgatggtgcg aatcttgtac tccgtgaaac tgtccagcac
    34981 cgcttcgata tgtccgccgg aaattccgaa tacgatatcg atattttcca cttccttcag
    35041 atattttcct accagatgtc cgcccattat ttttgccatt gtttttctcc tttaataatc
    35101 agttactgac gataattaaa aaatttaatc tcattttttt ggacaaatca tatttctcct
    35161 cccttgaggg aaggcgttag aggagtaaaa ttcacatcac cagtttcaca ccgtcattga
    35221 accactgcgc gaaaaaagcc atgccgtcga gcttcatctt gcccgcggcg gcagctttga
    35281 actgttcttc atcactcttg gatgccatca ccctgaaccc cgtggccgca tccgaccaga
    35341 caatggccga atcatacgga tgatcggcgc cgcctttgga acttaccgtc ccccggtcaa
    35401 agataaataa tcggccccgt ttaccgtccg ccgtcttgat catgattctg acctgatgaa
    35461 tagagccgac gtaactgcga tagaccggtt tcattttcgc cgcccatttg aggatttgat
    35521 acaaaatgaa caacagcgct gaaaatctca tggtgctccc ctcccccgat gaatgttttt
    35581 attcgaccct gatgtgtgac gtgtttatgg tagtttaacc aaaattaata aacttgacaa
    35641 tgaccgggaa agacaattat attgtcattt acggccagga taaaaaccat gaatcaacaa
    35701 tccagagcag attccatcca tctgatatcg agcccgacat tgccatattt ccgctccgtg
    35761 aacgggattg cgattctggc ccgttatgtg gaaaagtacg gtatccatgt caatactctg
    35821 ctgaccggca gcggacttca tccccgcgat ctggacgatc ctgaaatgtt tgtcacgccc
    35881 gagcaggaac tcgcggtgat gcgtaaaatt ctttttctgg tgccggaccc gaagatcggc
    35941 cttattatcg gacaacagta ccatgtcggg gtacagggca aactgggcgc cgcagcaatc
    36001 ttcagcgaca ccggactcga agccatcacc atgtttttca agtatatcgc ccttaccctc
    36061 acctattttc aatatgagct gcatgtcaaa gacaaactgg cttttgtaaa cgtgagagaa
    36121 ctgatcgacc tgaaagacct gcgtgtgttt gtctgtgagc gagaatttgt ttccatctac
    36181 cggattgtca gcgatatcct caaggccccc attccggtca atgaaatccg gtttgcttat
    36241 ccgaaaccat cctatgcctc tttctatgag ggtgtctttc attgtccggt caaatttaat
    36301 gcgcctgaac acaggattat tttcgacagc acattgcttt ccatgccgct gcccatgtcc
    36361 aatcccatag ccaggaaaac gtacgagcag gaatgcaagc aaatgtgtca acggctgaag
    36421 gtaaaagaga cgatcaccga tcaaatccgg caggaaattc ttttcaagga ggaaagtgtc
    36481 ccgagtttca cgcaactttc ccggcacctg aatctttcac ccagaaccct cagacgtcgc
    36541 ctgacggatg aaaagacatc ctataaatcg ctggttgcgg atatccggaa aaagaaggcc
    36601 ctcgattggc tcaagacgtc ggactgtccc cttgagacga ttgccacaag actgggttac
    36661 agcgatatat caaacttcta ccgcgccttc aaacgctgga ccggacgcaa tccaggcttt
    36721 taccggaaag agcatggcag gatatgatga tcatgacatg ccgccttcat ctgaaaggtc
    36781 aaatgtaaaa aaagtatacc gtgaaagata tgatcatttt tgaataccga cttggacaaa
    36841 caaaagaggt tcttcaaaaa cttctgcgct ctattcacca tcttcgtatt tatgcagtct
    36901 tttacgcagt gtttttcgtg taatacccag acgccgcgcg gcttcacttt tgttgccgcg
    36961 acagctttgc agcgcctcca gtatactttg cttctctatt tcctccagag aacgattccc
    37021 ggaagctgcc ggcattttct gccccgccga caatacctcg cgagagaggg cctgatccgc
    37081 catggtaaaa atcaggtctt cttcaccgat ggtttccgtc ctgcaaagaa caaccgccct
    37141 ctccaccgcg ttcatcaact cccgcacatt gcccggccag gaataactcg ccagcttctg
    37201 catcgcctgc ggcgtaaagc ctttgatgac tttggcattg ttttccgcaa acattttcag
    37261 aaaggcgtgc gccatgagag gaatatcctc ggaccgctcc ctgagagggg gcacgtccag
    37321 cgtcacgacg ttgagacggt agaataaatc ttcgcgaaaa cgtccgtcgc ggatttcctt
    37381 tttcaagtcc ttgttggaag cggcgatcac gcgcacatcg attttaagaa catcgtgtcc
    37441 gcccacgcgc gtcagttccc gctcctgtaa tacgcgcagc agcttaacct gcatggcggg
    37501 cgacatttcg ctgacttcgt cgagaaaaat actgccgccg tcggcctgaa cgaacttgcc
    37561 ttcccttcgc ttatccgctc cggtaaacgc gcccttctca tgaccgaaca gttccgattc
    37621 cagaagcgtc tcggtaagcg ccgcgcaatt gatcttaaca aacggcgcgt tcttgcgggc
    37681 gctgttgaaa tgaatcgcat tggcgatgac ttccttgccg gttccggatt cgccggtaat
    37741 cagaacggtt gcctgtgtgg cggccacctg ttcgaccacc tccagcagtt tgatcatcga
    37801 cgggctctgt ccgatcatgt ttcgccggtc gaatttttca cccagtttct ctttcagata
    37861 ttcattttcc ttcttcaagc ggctgtgctc ggtcgcccgg gcaatggata cctgcaattc
    37921 gtcgaagtcc agcggtttgg tcagataatc gtaagcgcct ttcttcaggg tttggacggc
    37981 ggtttccacc gacgcgtatg ccgtcatgat aatgaccggg atgtccgcat taatctgttt
    38041 gatctgttca agcgcttcaa tgccggatac attcgtcatc cggatgtcca tcagaatcaa
    38101 atcaaaagag ctcttccgga ctttttccac cgccaccaga ccgtcatccg cttcggtcac
    38161 attatacccc cagccgttta gaagcttttt cagcatgacg cgatgcgcac gatcatcatc
    38221 cgtaatcaaa atatcgagcc tggttttcat tatgtcttct ctctttcccg aaaaataaat
    38281 attatttctc aagccggttg aggaaatcag gggctttgct ttgccggaca gttatgatga
    38341 aagcattcac gttaaacagt ttgtccgccc catgtcctgc atcgtatttg cgaactcaat
    38401 gactgatgaa ggtttccaga tttcaacacc cttcacttct taaaacgtcg tgtcttacga
    38461 cacggcaaac cgcaacgtga ccttcgtgcc cgcacccgcc tgactttcga tgttcaaaaa
    38521 agcgccgtgc gcttccataa tcttttgcac aaccgccagc cctaggcctg tccccgccgg
    38581 tttggaggta aaataaggat cgtagatccg gggcagatca tttttctcga tgccgctgcc
    38641 cgtatcggaa atgagcactt cctgcccggc ctgtgtttgt gagatcccta cggacaagcg
    38701 cccgccgtcc ggcatggccg ccagggcatt cagaaatata ttcaaaagca cctgcttgat
    38761 tttatccgga tcgatctcaa cagccggcag atcttccggc acacgcgcat ccacctgcac
    38821 gccgtttttc cgcgcttcaa cttcaatcaa tttgacactt tgcatcacca aaccggatag
    38881 ggcggttttt tctgtcttca gcgtcagggg tcgcgcaaat tcgatcagtt gaccgatgac
    38941 gcggttgagt cgttcagttt cggcaatcat aatatccgcc gtttcctcat catcgggatt
    39001 tccggcaaac cgctgtttga aataaaccgc gaatcccttg atggaactga gcggatttct
    39061 gatttcatgc gccacacccg cagccagcga tccgatggcg ttcagatgtc ggctgtgcgc
    39121 cacttccttc tccagccccc tgacggctgt cacattacgc gccagcagaa ttttcccctc
    39181 cggaacatcc cgatcggcaa aagcggccac caccacttcc catatctgtt ctccttttgt
    39241 ttctgaagaa agcacgacat cttcttcgat cagaccggac gacatcgtca cctttgtcag
    39301 caaccggacc agcgggcccg gaagtatccg gccggcatcc tgtctctcaa cattcgacgg
    39361 atcaccggcg aacaaatcac gggcgtgttc attggacgcg ataatccggt tgtctttgtc
    39421 cacggccaga agcccgatgg gtaaatttct caccagcgct tctgcgaaaa cccttacgcg
    39481 cgacagtgac gcacgtgtca accggtatgt ctggaccaga aacaatgaca caatcgcaag
    39541 cgatccgatc agaatcagaa taaacgccct gatgatggtc cggtaaatat cttcctgcga
    39601 cgccttttcg atcttatcca tgttgaatcc cacaaacaca atcagcgaac gatgatgtaa
    39661 cgccgcgtct gtttgccgcc acgggaacaa tccccggtag atttcaaatg ttcccgcgcc
    39721 gccgggattg gttacctgac gccagtgcat gtccttgctt cgggcaatct tttccgtatc
    39781 cagatctatg ccatactgct gatccagcat ggagggatcg ctgtccgcca gaatctttcc
    39841 cttgctgtcc gtgacgatca tgtaatcgat atccggctgc tccgccgttg ccatcagcag
    39901 tttctggaaa taaaaacgat cgtttttgcc atccacacga tcttgcaatc ccgcctcaaa
    39961 agattgaatc agcgttgcgc ccttttcgat aaaaagcgcc tgagcctgtg tctcgctctg
    40021 ctgaaactgc atgaacatca tcatgcccag aatcaccatc aaaacggtaa tcgcgctgac
    40081 agccgcccaa atggaaatgt cccagcgtat ttttttcttt ttctcctgca tcattacctt
    40141 gtcccgccat atttgggcac gatggaccca tctcaatatc cgagccgggt atttattatc
    40201 cactcattat tcataaaagg caaacagaaa acattccgta ataaaccttc gctaatcatt
    40261 acaaatcctt atcatcacgg caaggatttg ccctcatatc cggatcatca aaaaatatgc
    40321 aaatcatacc tcatcgtgcg gaccttgcct gcataatatc tgtcgcatga cacgattgca
    40381 tctttttaag gatgtacctg tttatgaaga attattatta cgggcagggg atttatcgga
    40441 cggcaaaata attgatcaga aaaatcatta atcggcgctt acttaaaaac ccttactttg
    40501 ataaagctca tcacttattg gcatgccttt tttatgaggg cgttgaagaa cggattatcg
    40561 ggtatcttaa cccactttgg attatcaatg acatcggagc tgaagagctt accgtccttg
    40621 tcaaaataaa aaatggagac atgtctgatt ttcttttctc tgcaatctat ttcattcaag
    40681 gattgaacat tcgatatttc ttcatatccc ccggtggaaa gttttttgtt ggctctgtcc
    40741 cggataaacg cctctctgcc tttgtcggaa aaaacatctt taatgacata tttcacggcg
    40801 ccgtctttag aaacgacttt tttgtaaaaa gcaacattcc ccagattgcc cgtgtaaaat
    40861 tcaacccatt cggacgcgcc gaatacaggg cttgccggat gaaagaagat catcaacgtg
    40921 agaagcataa cccaacgcat tgttttgatc acgatgcacc tctgaaaaaa gaaaatttct
    40981 ttgatattat actaacacaa taacgaatta aaaaaaacag gcatgcacac tacccgggaa
    41041 aaacatacgg cggcggatcc gttcgcagcg gatgggccgc caagcggacg ccgcgtcaat
    41101 attttgatac atccaccgtt cggatcgaac aaaacgatat gttctttaac ggcttacccg
    41161 ctggatagaa gatacgagcg aagattaatt ttggaatttc tcagattcaa tttttttctt
    41221 agattgtttc gatgggtcgc aatcgtcttg gttgatgcgt tgagcatctc agaaatttct
    41281 tttgttttct tccccttttt gatcaggctg ataatctgca tttcctgagg agtaagatga
    41341 ttgaaggagg attggagatt tttcataaac ggagacaaaa cctcgttcag attgctttcc
    41401 agaatgtcca gataattttt actgcgtccg tcaaggttca aacgtttcag ttttctcaaa
    41461 taaggcatta cgagatcacg catgttggtc tgcaactttt cttcgatttc ctgtttgtct
    41521 tcatcccgtt gattcatcaa cacacgcagc gtgatattag cctcttccag atcttcagca
    41581 aaacttttaa gctcatcctc tgcctgcttg cgctctgtga tgtcatgcgc aacaacatcg
    41641 gcagcaacca cgcggccttt cacatcgcaa acatttctaa tttgcagttg aatataaaac
    41701 gactcgcctt ttttattcaa tatttcaaat tccaccgagt ccggaacttc caaattttga
    41761 gacaatcttt ccacgcgttc caaaaacctt tttttgcttt cttccgtaag aatgtcgaat
    41821 gccgtaagag acgttacctc ttcctgacta cagccgacat atttacaaaa aacatcattc
    41881 gctttcagga atcttccggt ttgataatcg atctgatata ttccggcagg cgaattatca
    41941 aaaagttgtc tgtatttttc ttcgttctcg cgcaaccttt cttccatcat tttacgctcg
    42001 gtaatgtcaa ccacgactgt aataatgtag cgttcatcat caatggtgat cagttctgcc
    42061 gattggagac attgaagcat tttgccgcct ttgacctgat gacgcacttc catattcctg
    42121 tatcttccgt ttctcagaag acgttttttt atttcttcac gatcctgatc gagccatatg
    42181 cccagtgttg aagccctctt gccgatgacc tgttttcttg caaaccctga tttctcgcag
    42241 aaagaatcac tcacatcata gatggtgccg tcctccagtt ttgaaacaac gatggtattg
    42301 ggagacatgc gaaatatcgt gcgatagacc aggccggttg acatccgtgt ctgcccgact
    42361 tttgtcgtgt tcaggagatt ttccagctct ttaattttat ttttcagcac cttaatttct
    42421 tttgacgcct gattcctgcc ggtttccttc atgaaaaagc acctcgtccg gtttgatata
    42481 atttttttat ataaacaagc ctgtcgattg tcaacgtaca cgatcaatat ccgtcattgc
    42541 gaatccctca taactaatcc tgacgacatt tcatttacgc acggttgcgt cattttcagg
    42601 aggcaaatat attctcgctt aaaaaatgaa cgggatgaag cgtttggttc gtgtcatata
    42661 tacgcgatac ggtccgccaa tcgtatcgat cagagctttt tcctctgctt ttactctaag
    42721 gctgtagaca aaacaattct ccaggaaaag aacggcaaaa ctcaaccagt tgcccagggc
    42781 cataccgatc cccgcaaaca tgataaaagc tccgctgtat gccggatggc gaatccagcg
    42841 gtaagggcct tcttcaataa ccggctgatc cgcctttaca acaacctccg gagtaaagta
    42901 ttttcccaga gcacgaatgg aatattgacg aaatatactg cctgcaatga gcatgcccgt
    42961 tccaacggcc agtgccggac gctgccatgt gatgacaaac cagggtgtca gcgatgccat
    43021 taacgccaga acaaaagcaa cgcgataacc gattttgatt aaaagacggg tcccggcatc
    43081 ctgcgtgttg gaaggatttc tggcaagggc gccggcaaat ttaatttcca gataaaacga
    43141 tccgattaac gccgtccaaa aaagaacagc ttccggatat acaaaaaccg gcggtatatg
    43201 catgtcgcat gtctccaaat gacatatgtt tgaaatgtcg tttcatgttc aatctccaac
    43261 gttcagaaga agggaaacgg gacatcagtg ttttttcggg atatcctttt tcatctcatg
    43321 catggcctgc atcaactcat cgatgtccac ttccgggttt tcgccgtatc cctgcataag
    43381 cgattcgtca tacatctgtt ccagttgcct cagttgcacc agagcctcca gattctgcct
    43441 gaaatcattt tccatcattt tgttaacggc gtctccggat tttttcagat cacttaaatc
    43501 gatgttcagg gatgtgcggg aaagaacttt tttcaaatac tcgcagccta tttttgcatt
    43561 aaacgtttca atatacggcg ggactctggc gacaatgctg gccgcctcta tcccttccct
    43621 gccggcatga ttcaccagca gcgtcgaaaa gctgcatggc cccgtgtaat tcaggagcgg
    43681 gaatgattga aactcatgct tcatctccgg acggctcaac acggcataaa cgattgtttc
    43741 ccgggtgtgg ggaacctggt caaagacgcc gcccagaaca tagatccgac acgcctgata
    43801 acgtttggcg atgttcaaaa cagccgatgt gaattccggc catgcgacgt tcggttcaaa
    43861 accggaaaaa agaatcaggt cgttctcttt accgctcttc cagaaataaa attcattggc
    43921 gggctcgttg agcgatttga tcaggcctct ttcaacgacc gcatgcggac gcatgacctg
    43981 cgccggatcg aatcccggta cctgccagat atagaaattc cggatatcaa tggaagcgaa
    44041 ctttcgggca tcgagtttgc ggcgcagata gtcgatgctt ccggtcgcca tctcaccggc
    44101 attgagccag ccgcggtaac cgattagaat atagggattg ataaggtcgg gcgtttcctg
    44161 aaaaatgaga ttgtcgttca taagccctcc tgagatggaa cttgcagttg cggttttaaa
    44221 aacccatact acatttaatt atggtttgtc cgttcctttc agtcaagtaa ttaatgaaac
    44281 cctggtttca gaagagtagc acacaaaaac tcgtagttga attatcagtt atggtaattt
    44341 gagttgctgg tggtagtggg ggcaaagaac ccaaaatggt ctttctcccc gaagggcgtt
    44401 attacggcag cttgtaccgg tcgttcctat gccgggaaat caacaacctg ctggttaccg
    44461 gcagagcgat ttcgatggat cgcggcgcgc tggcctcggc gcgtgtgatg ggcccgtgcc
    44521 ttgcggaagg acaggcggcg ggaacggcat gcgcgctggc tctttcgtcg ggacaatcga
    44581 ttgatgccgt tgacaccggc aagcttcggg aaatgttggc caaggatggc gcacggctga
    44641 aatgaactta aaaaaatcgt tgaatgttct tcgctcaacg ttccttgttt aagaattaaa
    44701 aaaaatgaca attttaagtg gaacgtggta aaatataaaa aagttccata cagatgttcc
    44761 tgattccctc tcctgctcag aaaacttgat cccgctcttt tgactggtct gtttgcggaa
    44821 gcattgaacc ccgtcaacac aattcaaagg aggtcactta tgccggccaa aaaattgaaa
    44881 gagtttctgg acagccatgg agtgaaatat gtgaagctca cgcattcgac ggcgtacaca
    44941 gcccacgaaa ttgctcaatc cctgcacatc agcggaagga aggtggcaaa gaccgtcatc
    45001 gtgatgattg acggaaagat ggcaatggcg gtcctgccgg gcaatcgctc cgtcaatttc
    45061 gatcattttc aaaaagccgt cggcgcgaag aagatcgaac ttgccaccga agcgcaattc
    45121 aaaggcatgt tccctgattg cgagctcggc gccatgccgc ctttcggcaa tttgtacggg
    45181 atggaggttt acgcggatca gcatctgacg gacgatgatg aaatcttttt caacgcctgt
    45241 tcgcactcgg aactggtcga gatgtcttat ccggattatg aaagactggt gaaacccaac
    45301 gtggtgaagc ttacgtgata gcgttctaag ttcaacgttc aataaaacat gaatgggaaa
    45361 tatcaagaaa ttcaggttga atgctacagc ggttttaagg cgaatgaacg cccggttgct
    45421 tttacatatc agggagagcg gcaggaaatt caggaggttg ttgatcgctg gtatcacgga
    45481 ggtctgtaca gcagcaaacc cgttattaat ttcttcaaag taaggaccgc agacggcaag
    45541 gtctatcttc tgcgctatca gtttgattcg gatgcatggt cgctgcggat atgatgaccg
    45601 cagacgtcag gatgatgatt tcagaatctt cgttttcagt ccggtgtgaa aagattgatc
    45661 ttatccttgg aaacgactct gcccgttaca ccgcagaagt ctttctcaat catggctgaa
    45721 aacagagacg gatctgaagg tgtaaaagcg gcaaagcgtt tcccgttttc ttcttttccg
    45781 atgacaatac ccttgttcgc caaattgtcc cggtcgtaaa tcacggtata ggtgtccacc
    45841 gttgcccgcc ccgaaggcgt cggatcaatc tcatatttag gccgtttatc gactgcctgc
    45901 tggcacgttc gtgtatcggc tgtcgccgcc gcattgttct cgggagaccg ggtagagcac
    45961 acggctgttg agtgcttggt catgtaaaaa gaatttcccg tgacaagacc ggtcctgccg
    46021 ggatgccggc gcagaacctc aaccatttgc gccatggagt gcatgacata gttgtttccc
    46081 ggtccgccga aatagggaag tccccctgta acggtcagat cgcggctgtc attttcagga
    46141 atacccagca tgtctcgggt tacctgcacg gcaaccggaa aacaactgta cagatcaaag
    46201 aaatcgatat cttcgatggt taaaccggcc tgcttcaggg ccgtccggcc tgcgatttca
    46261 atggcgggag aaaaaccata gccttctctt tccaagacat tccagtgatc gttggcttcg
    46321 gcataaccgt ggacatacac ccactttaac ggattcacgc ccatcttttg tgcctgtgct
    46381 tccgatgcga caaggagtgc ggcggcctga tttaccaaca gcatggcatt catccgtttg
    46441 gtatacggga aagcggtgtt gcgattcgtt tccgaaaggg tcatgatttc ttccggcgtg
    46501 aacgaatgct gaaaccaggc aagaggattt ttggcagcca ccaaggagag gcggctcatc
    46561 agcctgccta tcttttctgc atgctcttca attgtgcagc catagcgccg gcgaagagca
    46621 ttctcaaaca tgggatagac ataagcggga agtgaaagtc cgtgaatgtg ctctgtttcg
    46681 caggcgccgt caaacagccc cagcggagtc agtggcggca ggcagggccg accggacgcc
    46741 acgttccagc cggtaaaggt tttggaattt ttcgcctgct gcatggtgtg ttgagcctct
    46801 gctccggtca aaataaccag accggctttc cctccggcaa tctcctgcat ggcatgatgc
    46861 agcacggcgt gcggcatgac accgccaaag ccggaaactc tgcatcgtgc gtcggtcaga
    46921 ttcagtttat cggccacgca tcccggcggg ttggcaatac cgtcatcgga aaaaagcgac
    46981 gtggtcatca gcaggtcggc ctgttgtaag tatttttctg tcacgccgcc atcttctgcc
    47041 gcaattttca cggccgctgc cataaggtct atcggcgaac cgccttgcac agatgatgcc
    47101 tctctttttg tcacctgccc tgccccgata atcaccggaa tattttcaga attcattttt
    47161 caacttccgt ttatttcttt atttttaaag atgttttact cttgttgcct ctgccgaaca
    47221 acacacgcag ttcgtctttg gcctgttcca atattgcttt tcgttcggcc ggcagttttg
    47281 tgcccgcgcg attgatataa tagttcagca tggacatggc ggattgaaaa ggttccgctt
    47341 ttctttgcgt agagctctcg gcggattctt taagcgattc ggcaatccgt cgcggattgt
    47401 caaaagtaaa tacaccggat tgcaaatcga gggcaaagct ggtttccttg actttttgcg
    47461 tccagctttc ttttgttgct ttcttcattg caaaattata ccagattcct gccgtgcatt
    47521 ccatattcgc ggctgatgca ggctaacgtc agaattactt ccggtttccc attttgacga
    47581 cggcgtcgaa ctcgtcggtg atttctctgg caggcgcttt gctgagcagg ctgacgacga
    47641 tgattgccaa acaggcgaaa acaaaagcgg gcagcagctc ataaatgttc caaacgccgc
    47701 cgaggggccg tacgagatat ttccatacaa agaccatcac gcctccggaa accatgccgg
    47761 ctatggcgcc gtatacattg gaacgtctcc agaaaagggc aaacagcata accggaccga
    47821 aggccgcgcc gaaaccggcc cacgcaaacg aaataatgac aaaaatcgag ctgtccggat
    47881 ctctggcgat gaacgcggca atggcagcaa tcacaataac ggccgatctg gcaatcatca
    47941 tgccggtttt ttcggaaagc ctaatgtcga aacattcctt tatgagattc tcagtcacgc
    48001 tggaggaggc caccagaagc tgtgagtcgg aggtggacat ggtggcggcc agaatgccgg
    48061 aaagaacaac accggcaatt aaagagctga tcacgccggt gtggctcaga agctgtgcca
    48121 tccggatgat gattgtttcg gatgcgcttc cttccagtgc ggcaatctca ccgttgaggg
    48181 acagggtttg accgacaatg ccgatcaaaa cggccacggc aagtgaaacc acaacccagg
    48241 tcaccgcgat ctgccgtgaa aaaacaattt tcttttcatc gcggatggcc atgaatcgca
    48301 aaagaatatg gggcatgccg aagtaaccca gtccccaggc cattgtagaa atgatggtaa
    48361 tcagtccgta gcgttccgtc tgaccggtcg tggaattatg tgaaaacatc atactgagat
    48421 aaccgggaat agcatccgcc ttttgccaca cggcatcaat gccgcctgca acagcagcgc
    48481 cgaacatcaa aatgctcaac agcgcaatgc tcatgacaat gccctggatc agatcagtag
    48541 tgcttgcggc aagaaatccg ccaaccgtcg tataggcaac gacaacaacc gcaccgatga
    48601 gcatggccgt tgtgtaatca agaccgaaca cactattgaa aagctttccg caggaggcaa
    48661 aaccggcagc ggtatacgga ataaagaaaa tcaagatgac aatgctggcg atccatgtga
    48721 gaattttttt ggaatcgcga taacggttgg agaaaaattc cggcagggtg atggcgtttt
    48781 gcgacagttg agaatagcag cgcagacgac gggctaccgt aagccagttg acataagtgc
    48841 cgatcgccag accgattgcc gtccaacccg catccgcaat accggacaga tatgcaacgc
    48901 cgggaagtcc catgagaagc cagctgctca tatctgatgc ttcggcgctc atggcagtga
    48961 cgatggtccc aagttttctg ccgcccagat aaaaatcgcc gatctgatca ttgcgtctgg
    49021 caaagacaaa gccgatgacg atgaccacca ccatgtaggc ggcaatggac atcagcatat
    49081 aaaaattgaa gtttcccgtc ataatctctc aaccatccct tcaaccatta gtcgggaatg
    49141 aaaccattaa catgccgctt attatttttt ctgacgccat aacattgctg caatagttat
    49201 gctccccgga accttaaaaa gttttgttac aattttgtcg atcattcgtc aaccgccagt
    49261 tgcgctcaat aaaggttctt gacaattccg gataaaataa actaatgatt taacgttaaa
    49321 tgataaggag atactatgtt ggatgtaata gaaaaagctt tgaaggaagg acgaaagact
    49381 ctttcggaat acgaatccag gcaggtaatt gaatcagccg gtgttgcggt ggcggtagca
    49441 gcgctgacca aaaccaaaga agaagcgatc cgggaagccg aacaaatggg ttatcccgtg
    49501 gcgatgaaag ggtgttccgc cgaactttcc cataaaacgg aagcggggat ggttgcactg
    49561 aatgtcaaca gcgcgcagga cgtcgcacag gttttcgacg cactgaccgg caaggtaaaa
    49621 aatctcgacg gcgtgctggt ggaaaagatg gtcagggggt cgcgcgaact ggtgatcggt
    49681 ctttcgcagg attcgagttt cggcccctgt gtgatgttcg gcctcggcgg tatttttacc
    49741 gaagcgctca aggatgtgac gtttcgggtt gccccgctga cgaaacagga tgcgctggaa
    49801 atgatcgatg aaatcaaaac taaaaaatta ctcggcgaat tccggggcag cccggccgtg
    49861 gaccgaaatg ctctggccaa agcgcttgtg ggcattgggg atctggccat caagtatgaa
    49921 gccatcgccg agatcgatat caatccgctg attgtctgcg gagacaaacc ggttgccgtc
    49981 gatgcgctgg tggtattgaa gtaacgctgt cattacagaa cactttttga acatcgtgac
    50041 aatccaaaaa tattttggtc cctgttctca acatgacaat tcattgccct gcacgtgtgc
    50101 cagcaatgca taaaacgaag atttgggaga atgtaatgtt agattatttt tttaatccga
    50161 aaagcgtcgc cattatcgga gcatccagca atatgaataa gggcggctat catatcatta
    50221 aaaatattac gtccggattt cccgggaaaa tctatccggt caacaaaaat tacccggaca
    50281 tgctgggact tccctgctat aaggaccttg cttcaattcc gggcaatatc gacctggcta
    50341 tttattttat tccttcgaaa gaattgcccg acacggtcaa tgaatgcgca aaaaaaggcg
    50401 tcaagggcat catcatcgaa tcaggcggat tcaatgaagc cggagcagaa ggcgccagaa
    50461 ttcagaaact tgccctggat aatgccgccc gagcgggcat ccggttgtgg ggccctaact
    50521 gcatgggctt tattgatgcc aacaaaaggt atgttttttc ttttatcaat tctctggtct
    50581 ggccggacgt tttgcgcggc ggcaacgtcg gcatgattgt gcaaagcgga atgctttccg
    50641 ccggtttcct gctgcatgcc ctgcaggaag gcatcatggg cgtcagcaag gtgtgttcca
    50701 tcggcaataa gtgcgacatc aacgaaaacg acatcctgga acatctcatt gacgacaagg
    50761 atacggaggt cattgcctgc tatctcgaat cgctggtgga cggaagaaag tttttggacc
    50821 tggcaaagaa gacagaaaaa ccgattattg ttctgatggg cggccgcagc caggaaggag
    50881 caaaggcggc gcaaagccac acggcaagcc tgtcgggaaa ttataaaatt gccagcgccg
    50941 ccttcagaca ggcgggcatt atcgaagtgt ttgatccggc ggaactgacc gacatcgcac
    51001 gggctttcgc caagaacatg ccttttcatc ccgaaaagga aaagggcacg gctatcctaa
    51061 cgttctccgg cggcgcggga accgtcacca cagacctgat ggaagactgc ggtctgaaac
    51121 tggcaaaatt gagcgaccgg accctcgcgg cggtcgcgga acttttcccg ccctggaata
    51181 aacccgacca tccgctcgat ctctggatcg ccatcgaacg ccacggcttc gaaaaggtat
    51241 tccggaattc tctgacggcg gtcatcaacg atccgggggt cgattcaatc atctttcaca
    51301 gttatgcctc gccattgatc ggtcaggatt tttttgaaga acttgctgcg atgattaaga
    51361 aaaacaaaaa accggctgtg ttatggatcg aaggaagaaa ggatttcgcc gaacatatcc
    51421 ggggtattgc tgaaaacgca ggcctgcccg cttatcgcga aatgaaccgc tgtgttaccg
    51481 tcatgaacgg catgaaacgt cattttacga aaaaaccgat gagctgaaag gagatcgcct
    51541 tggcacgcat acctgatttt caaccgggtc cggctgatac ggaaaagaaa tttcttcagg
    51601 gcaaaccccc ttttgatttg aatattttca agatcatcac gcacgccccc gtcgggattg
    51661 ccagggagtt tatcggactt ccggcatctg tgctcatggc ggggaaactc gatccggttc
    51721 tgagagaaat ggccatcacc cgcgccggca ttctgtgcaa ttccgcgtat gaggtgcatc
    51781 agcatcgcaa actcagcaaa ctggtgggct tgcccgaaga aaaaattaaa gctctggata
    51841 ccggttcagc atccccggtc ttttccggca tcgaaaagct cgttttgcgc ttcaccgaag
    51901 aaaccgtcct gcagcgagag gtcagcgatg caacgttcgc cgaaatctcg agacattttt
    51961 ctgaggattt actggtagaa ctttctatcg cggtatgctg ctacatggcg gtcagcattt
    52021 tcctcaatac gtttgaagta aatcttgaag aaaatgatgt acccttttaa ccttaataat
    52081 tgttttcttt ccatcagttt cattcatctt gacagcaatt ggaatcctca tccggttttc
    52141 cttttattac gaatttaaaa aacgaaaata atttgaaaat atgcaaattt ccccgctata
    52201 aaaaacggaa tatgtttatc gtaaaacgat ttttccgtaa aaacgattac agcccaaatg
    52261 gtaaaaatgt aagcaattga tattattaag tattattatt gcataaacct tgtgcatatt
    52321 ggtggaaatt cgtaataatt gaacacttat ggcgccagtt aacagggtta tcaacaggtc
    52381 tttccacaat attgtggaaa gaccttcatc acggaataaa aggttaaaaa ttaaaaatat
    52441 ttttgtctat ttagattttg ttggacaata aattcgatga acagataaaa aaattatgca
    52501 gcacacaacc attgtaccgg acgttctgaa acattttctc cggtcacgaa cagtatccct
    52561 tgccttttac cttctccaac aggtcgcatt ttcctaattc acatgctttt tgcgcatcat
    52621 tgcaaccatc ttctttattc atatgatcaa aataaacgtt ggctctgttg taataggcat
    52681 ccacgtaatc cggctttatt ttaacagctt cgctgaagtc ctcaacagct ttctgatatt
    52741 gacctaaagc aagataagcg gcgcctctgt tgttgtggat gatggcacta tccggtttca
    52801 ggcggatggc ttcattataa tctgcaatgg cttcctgatg tttcccgagt ttactcaaag
    52861 ccaatcccct gttgttataa ccgtaaatcg attgcggttg cagtttgatg gattcattat
    52921 aatcttgaac agcttccgga tactggccga tttcaatgta ggcgtttccc cgattgttgt
    52981 aggcatcggc ataatccggt tttaatttaa tggcttcgct cagatattca atggccttcc
    53041 ggggatcggt gcattttccg ctgatacaca gttcaaatgc tttgcgaaat aatccattcg
    53101 cctcatcacg ggcgggtgca gcagctatca ttcggttatc ggaagctgcc ggctgattta
    53161 caggcgcata aacggcttgc tgttcgaccg cctgtgtgtt gctcacttca actttctttt
    53221 cactacattt ttgtacgatt gcaagcagaa taccgacaat caggatgatc acgactatga
    53281 aaggatattt atttctgttc catgtttttc cgaaaccggt ttgacgttcg attggagctt
    53341 tgccttgtct cttcttccgc gaatctttga caatcttagg ctgtttcgcc tgctggtccg
    53401 gttgcggcag ttgagatgcc gacgctggcg tcaggggttc cagcacaaca ttgcaataac
    53461 ggcatttttg agttttatcc ttaaagcgcg tctggcaaac aggacatatc ttcataatga
    53521 tgtctccaac agatatcctc taaccatcgt ccgaatattt cttctcccgt tttggcatgt
    53581 ctggattctt cacgttttaa tctcgtgatg ctaccgttat aaattttcgg ggacaactct
    53641 tgttgccaca aaatgacatc cggcaaagcc ccaaacattg cagaattaaa ccgttttccg
    53701 aataacagga aatcgttgaa ttcggattta aattgtattt ggctattatc attttatgtg
    53761 gcctatgaaa agacaaaaac aaatattttt ttaatattcc taaaataaat aaaaaaagag
    53821 taggaataag tacaggtatt gtacttattt tcgctgatta tcgagcatcg ttttgatttg
    53881 tcggatgaaa tctgcgggat cttttgtttt caaggcgatg acttcaccgg ggcagactgc
    53941 tcttttatcc tgcgtcagaa tattggccgt gatgcccgca gcattcatga ggattccatg
    54001 atcttttaga aatctgtccg ccggagcgga agcaaccgtt gtgttcactt tcgcgataat
    54061 gcccgaatag acgaccagtt tggccgcggc cagtccgatg attttgtcgt gcagaatcaa
    54121 tccggatttt gcccggaact tttcaagcgc ctcgaaaagc ggacgcaccc ccttgccgtt
    54181 tgatgcatga attaacgcgc ccccctcgaa taaagccaga gaatattttg agaaatccgg
    54241 gatcagcata acagttttcg caatggtttg ttgatggcgc tgatcagaac ggcccataaa
    54301 atgtctgcca gaaataaaac
//
LOCUS       DAOSME010000008        52522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_824_length_52522_cov_21.894982, whole genome shotgun sequence.
ACCESSION   DAOSME010000008 DAOSME010000000
VERSION     DAOSME010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 52522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52522
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_824_length_52522_cov_21.894982"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1502
                     /locus_tag="PLG98_02145"
     CDS             351..1502
                     /locus_tag="PLG98_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOE32080.1"
                     /translation="MMTGSQKFLKKHVLDEGLCTGCGACVGICPYQVIYHDRTVQLHG
                     CDLVKGRCYDYCPRTPCDVGAIRERYCAAGDLTPEIGAVKGYYFSRAADPGLRNIGQH
                     GATVTAMLELALSEGLIDAAVVSSRDQLSLRKGVIVQNREAIRTHAGSQFTVSATVAA
                     FHQLVTKHTGQVGIVATPCQALALAKMKFNPGHEDIARVNQLTLVIGLYCGWTLSEEK
                     YLKLLTDKGIALESITEMDIPAGRNVLEIRTEDRLHSIAMEEAQTCIREACLYCMDST
                     AEFADVSVGSARFSGNWEDVRQWNQLIVRTTKGKELTDLAVEKGVLELREAPADSLKE
                     LKEAAVNKKKNALKNIIQKSGSAKNLLYLDSRDPAVLKYLEAEKKKSKK"
     gene            1806..2912
                     /locus_tag="PLG98_02150"
     CDS             1806..2912
                     /locus_tag="PLG98_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE32081.1"
                     /translation="MSEDIAILIAYVLLALLVSFLCSIAEAVLLSITPSFIEEQKEKN
                     PKRAILLKRLKQDNVDRALAAILTLNTIAHTVGAIGAGAKATVVFGSAWFGIFSAVMT
                     LMILFLSEIVPKTIGAIYWPKLVVPTVFYVQTLIVTLFPIVWISERMTKFISRGKNLH
                     IFSRDEFVAMARVGEQSGQIDDNEARIIKNLFRFGSLNVTDIMTPRTVIAALSEDKTV
                     ADAAEYVVKIPFSRIVIYKKDIDDITGFVLRDDVLLRDNQERGGEALKTLKREILVVP
                     ESAVLTDLLEIFLKERQHIAIIVDEYGGTTGLVTLEDLIETLMGLEIVDEMDTVVDMR
                     VLARKKWEERAGALGIKINVDGKDQSKKENVLPE"
     gene            2997..4940
                     /locus_tag="PLG98_02155"
     CDS             2997..4940
                     /locus_tag="PLG98_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOE32082.1"
                     /translation="MINKLLSPAKTEVLFMGNEAIARGALEAGVSVVSGYPGTPSSEV
                     IERLSEVARERNLYVEWSTNEKVALEVAAAASFSGLRSMCVMKQNGVNVASDFLLHLA
                     SSGTRGGIVLVECEDPGALSSVNEGESRYFARMLEIPMLEPANFQEAKDLTRWAFELS
                     EKIRNIVMVRSVTRMSHASGNVICGSLPAKFRNARFISDGSVMDPLRGPVVSTPVVIK
                     HGLQQKKLQTAAELFEKSPFNSYVGPKKPELLIITSSACFLYSREALEILGLQKRVGI
                     LKLSCTWPLPEKLLKKYLRLTKKIMIVEEVLPFLEENIKVAAMTMLKDIGIKTFYGKT
                     DKTLPSAGELNPEIVMRALSKIMKVKPKSLPAGYLKEVQKVAPKIAPRAQTFCPGCPH
                     RASFWNINTALKLDGREGIVCGDIGCYAMASLWPEIGFHTVRTLHSMGSGTGLASGFA
                     KLSAFGLDKPVMAVCGDSTFYHAVIPALINAQHNQADITFIVLDNAGTAMTGFQPHPG
                     INESAIGEPLPPVDIEAVCRAIGAKVKVSDPFDLEGTSRILTSFMAERGSMKVLIMKQ
                     ACALSPEKKGKKQYQMKVNEDLCLGDQCGCNRLCTRIFRCPALTWDRKKQKAVIDDVI
                     CAGCGVCYSICPQKAIARVEAEA"
     gene            4937..5548
                     /locus_tag="PLG98_02160"
     CDS             4937..5548
                     /locus_tag="PLG98_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOE32083.1"
                     /translation="MKKSLKKNPTNIIITGVGGQGNVMASRVLAGMLVNAGFIVTIGE
                     TFGMSQRGGSVMSHLRVSSSSVLSPQIPQGRGDIVIALEPVEALRVLTKYGNPDVAVL
                     TNSRMVYPMGVITGEFTYPTIEEVKSMFEKISAKNWIIDATSVAVELGNPVLSNIVMI
                     GALAGTSLLPIDRRAFEKEIAKTIPAGKHQINLKAFDAGVKML"
     gene            5580..6323
                     /locus_tag="PLG98_02165"
     CDS             5580..6323
                     /locus_tag="PLG98_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32084.1"
                     /translation="MDADHGSSGKVTYNYRFIFKDGVEKEFNIALDSVTLNLLPTDKK
                     NYPEWTKLNCFRCSHCTLNDQCEYCPIAINMVDVVDYFRNYFSYDIVETIVTAQERKY
                     FRTVSLQHGVSSLIGIYMVTSGCPLMETLKPMVRFHLPFATIEETKYRAISMYLMAQY
                     FLYQHGRQPDWDLKKLAEAYENVRIVNEYFCKRLRMIQGQDASLNAVVLLDIFADSVN
                     FSMDGRMANDLEYLFKGYFESTPGANNAS"
     gene            complement(6396..7280)
                     /locus_tag="PLG98_02170"
     CDS             complement(6396..7280)
                     /locus_tag="PLG98_02170"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOE32085.1"
                     /translation="MKAEDIKKILIAGAGTMGRSIGLSCVTSGYAIVLYDINDDIPAK
                     ARDIMNKKIDKMADFGMMPKDKAESFKNNITATTDLAAAGADADVVSESIPEDPDIKG
                     DFFAELNKVCPARTIFTTNASSLVPSMFADKTGRPEKFLAWHFHPGFKLVDVMGHPGT
                     SPETVETVQRFSELIGHSPIVLKQEKAGYVFNSLLNPWFLAALNLASRDIASPEDVDR
                     SWKEITASPLGPFEMMDYVGLETVWRITDFWAKKRNNHQAKLSADLLKKYVDRGELGM
                     KTGKGFYDYTQKKSPGES"
     gene            7606..9666
                     /locus_tag="PLG98_02175"
     CDS             7606..9666
                     /locus_tag="PLG98_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32086.1"
                     /translation="MSDKINIGPSGRNIIFCMVLTALILAAYLQVHQFDFVNIDDSVY
                     VTENDYIRSGLTLDGIRWAFTTLYAEFWHPLTWLSLMLDYELYGLHAGGYHLTNLFLH
                     IISSLLLFGMFQRMTGAFWPSAFVAAFFSLHPLRVESVAWVAERKDVLSVFFWMLTLY
                     FYALYTEKPVFRRYLAAVFFFACSLMSKPLTVTLPVIMMLLDYWPLKRFASRRQNLFL
                     WQLKEKLPFFLLSAVFSLIAIYAQPKFPIDGWPFSLESRIINAIISFVLYLQKLFWPY
                     DLAICYPFFGRAPAGQIIAAVLLFFAVSAAVVWMMKKSPCLLVGWLWFIITILPVIGI
                     IPAGNNAMADRYLYLPSIGIAVMIFFGIPLLVENEKIKKFFLPPAALIFFAVLSGMTW
                     HQCGFWKNSEALFRNASRINEENALAHINYGNALAGEGRLEEAVVYYNKAWGMLPETG
                     GNVMAYNKMGIAENKLRQLIDVSLGNVYTKQKKYQEAVHHYTRAIAMRPITPDHVRTY
                     NFRGTAYSRLGRKKDAIRDFNEAIRLDRRDYRAYNNRGLLYAETGMHRQALQDFNQAV
                     LMNPNNAELYKNRGIACASLKLYQNAFEDFEKALTLVPDDANIYYNRGVAYIELGRHQ
                     EAFSDFNEAIRLKADYTNAYHYRGGIYLMYGYAASGCDDARKACQLGNCEILEAAGSK
                     NLCR"
     gene            complement(9742..10683)
                     /locus_tag="PLG98_02180"
     CDS             complement(9742..10683)
                     /locus_tag="PLG98_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32087.1"
                     /translation="MRKDIRNPDIHSSPWLLDLAWFYIQRKMLKRKLPLLASFKITYR
                     CNLSCRACPFHLRSDREKEHMSRDAAVHIMDLLNQLGTRMIVFEGGEPLLWRDGSYRL
                     HDLILYARKKFLRVAVTTNGTMPLDVPSHTLWVSLDGQRETHNTLRSNSFDQVRANIQ
                     NTRHPRVFIHYTLNRRNWQDLPFVAQWVQDTPALKGMTVQFFYPYHQGEDDLSLPPEE
                     RRAAIEKLLQLKKSGLPILNSAGRLRAMIDNRWSCHDDILINVDPDGTITKGCYVKNR
                     GNINCDACGFTPVAEASGALDFIPGSLYAGWRLFLKM"
     gene            11035..12246
                     /locus_tag="PLG98_02185"
     CDS             11035..12246
                     /locus_tag="PLG98_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOE32088.1"
                     /translation="MNDIYSAVKVTDHVYWVGAIDWTIRDFHGYTTPRGSTYNAYLVM
                     ADEITLIDTVKKPFMDEMLARIKSVVDPSKIKYIVSNHSELDHTGSLPDVIDFIKPQK
                     VFASGVGTKTLKDLFHDEREIIAVENGQSLNLGNMNLTFMETRMIHWPDSMFTYLDVD
                     ELLFSQDAFGMHLATLERFADEIPVSTLEYEGATYYANIVLPYSPIVLKALEKVAASG
                     LKISVIAPDHGPIWRKDIGWIIDLYKKWALQKPTAKAVVIYATMWHSTEKMARIISEA
                     LAQAGVKVSLISMNETHRSEAVYEVLDAGALIVGSPTLNNNILPQMADVMTYLKGLKP
                     VNKIGAAFGSFGWSGESVKHLEAMLKEMKVDIASESVAVKHVPGADELDQCQKLGKTV
                     AAKLLEKVNSQ"
     gene            12278..12436
                     /locus_tag="PLG98_02190"
     CDS             12278..12436
                     /locus_tag="PLG98_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOE32089.1"
                     /translation="MKKYVCGVCGYIYDPAEGDSEGNIPAGTPFEKLPENWVCPVCGA
                     AKDEFSPE"
     gene            12569..13063
                     /locus_tag="PLG98_02195"
     CDS             12569..13063
                     /locus_tag="PLG98_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOE32090.1"
                     /translation="MDIKEALSQAYTGEAKAALRLKVYAQKAEDEGYKQMAKLFRVIA
                     FSEEIHGARALKGLKEIKSTEENLAASFESETQVAEVAYDRFVKLAEAEGNKEAVLHF
                     SQSKDVEETHAKLYKQAMNHFMEERETDYFVCKICGYVADGVLPEECPVCGAKAKMFV
                     AFQG"
     gene            13246..13983
                     /locus_tag="PLG98_02200"
     CDS             13246..13983
                     /locus_tag="PLG98_02200"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HOE32091.1"
                     /translation="MAFLQMPDYRGADESRFYPLPYPHVVYRGDFFKVDENRITGQIF
                     KTDRVLLDFSIFGSVPVNSDDNAAREGMDDLDPTFELGPALKIKLWEHKQHKTRMNLT
                     LPARAFFSTDFSSVRHEGWVFSPRINIVTDDLIPRTGLNLGVSAGPMFADSGYHEYFY
                     EVEPRDARVTRPVYRADGGYSGSTVTIGLSKTYKHFIFHAFVSADFLQGASFEDSPLV
                     KRETSMMSGFNVSWIFFKSEKMVNAEK"
     gene            14048..14452
                     /locus_tag="PLG98_02205"
     CDS             14048..14452
                     /locus_tag="PLG98_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOE32092.1"
                     /translation="MIITRWLIITAAILLASQFVPGIEVDKLSTAVIAACVLGLINIF
                     IRPIVVILTLPLSILTLGVFYFFINAFLLELVAYFVSGFAIKNFFSAFAGSLIISIVS
                     WLANSFITSHKIVKKDDPDYIDLRKGDDGKWR"
     gene            14443..17100
                     /gene="mutS"
                     /locus_tag="PLG98_02210"
     CDS             14443..17100
                     /gene="mutS"
                     /locus_tag="PLG98_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOE32093.1"
                     /translation="MAVGATLDLTPAMKQYVKIKQQHPDCILLYRMGDFYEMFFEDAV
                     TAAPVLEITLTSRNKGKEDSVPLCGFPYHAASAYITKLVEKGFKVAICEQVEDPKKAK
                     GIVKREVIRIITPGLVVDEENLAPGENNYLACLCAEKNTLGLAFLDISTGEFQIGEFT
                     DRDYFFTVISGLDFKELLLPRNFPDRILLDTIEAQMPQLRINDLDENYFLTGQAGDVL
                     QRTFSTDVLDACGISEHPAALKASGAILSYVNETQKINPQHISAIRWYSAEKFLLLDD
                     TARRNLEIFTSIQNNTAAGSLFSLFSETQTPMGTRRLRWWMSYPLVDAEKIKTRLAAV
                     AELKDDPLRRANLRKTLSRIYDLERLAGRVSLKAANARDLVALKNSLLAVPEIKSLLS
                     DCTAPAICAIRSHITEMTDVVDVISRAIVDSPPQKIQDGGFIAAGYDEELDKLRLISR
                     DGKQWIAALEAEERKKTGINSLKVGFNNVFGYYIEVTKANAALVPDSYIRKQTLVNAE
                     RYINQALKEFEQTVLNAEERIKQKEQELFINLRDGLDRYIAEIQGNANLIADLDALAA
                     LADVAEKYHYVCPEIDDEETIDIQDGRHPVVESALKNEGFVPNDCLMDLRQNKLLVIT
                     GPNMAGKSTYIRQVALIVLLAQMGSFVPAAKAKIGIVDKIFTRIGASDSLIKGQSTFM
                     VEMTETAEILKNATPRSLVIIDEVGRGTSTFDGLSIAWAVAEYIHDFQGRGVRALFAT
                     HYHQLTDLVVTKPGAKNYNIAVKEWGEKIIFLRKIMEGGTSRSYGIEVARIAGVPADV
                     IMRAKEILRNLEKGEFDEIGMPRIVRGAREGKNANPQLSLFSRRETAIALEKKGQNLI
                     EALKNIDISSLSPLEALNKLNELKNKISD"
     gene            17570..18937
                     /locus_tag="PLG98_02215"
     CDS             17570..18937
                     /locus_tag="PLG98_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE32094.1"
                     /translation="MVVEKAVLIVDDDSVHRMILKKHLNDWGYQVNEADDGHVAVEAV
                     RRMPFDLIIMDICMLNVSGIEALGQIKAINPETPVIIMTAYASLETAVSALKKGAYDY
                     LTKPLNFDELRIAIGRATEHNRLKRENRYLRNRLDEKFNRHNLIGQSYPMVKLLETVA
                     QVAPTEANVLISGESGTGKELIANAIHFNSMRAETVFIKINCSALTETLLESELFGHE
                     KGAFTGAERRREGKFLQAHGGSLFLDEVSEMSKAMQVKLLRVLQERELTRVGGAEVIK
                     VDVRVIAASNKNLKEEVQNGNFREDLFYRLNVVSLNVPPLRERKEDIPILAQHFLELF
                     AGRNKKTIKAFTPRAMEKLLIYHWPGNIRELMNAVERSVVLSRTDYLDADDMTLVMEK
                     SHEVSGIAEQGEAIRNLSLESMEKKNILAVLADCRGNKSETARRLGITRKTLRAKLIK
                     YEMNR"
     gene            complement(18992..20224)
                     /locus_tag="PLG98_02220"
     CDS             complement(18992..20224)
                     /locus_tag="PLG98_02220"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32095.1"
                     /translation="MNNFFIFIFVFVLSIAVIISFIYTMWRMNRDEQKLISIIHGSPI
                     PTFVISKNHKVVYWNRALQAASKIKPSDVLGTDQHWRAFYTSPRPCLADLILDGKTAQ
                     IAELYPDRWSRSKLLEDAYEVTQFYPDMGEKGKWYRITSAGLKDSRGNLFGVMETLED
                     ITEQKLAQDELLRRTKLESLGTFADGVAKDFDSLLTAILRNVFLAKISASDEDKMLEN
                     GLAVAERAGLQAKELAHRLITFAQGGYPVRKLEKIEPVLHEAAESVFKNSAITCNITI
                     AEDLWPCEIDATQIKQVIENVLINAREAMPDGGSVQMTTENTTTGTNIRSLGQGNYIR
                     IIIKDSGIGIPRENLPRIFDPYFTTKKTKEHGGIGLGLAISDSIIKYHNGLITVESIV
                     NSGTTFSIYLPAKTTNGS"
     gene            complement(20632..21699)
                     /locus_tag="PLG98_02225"
     CDS             complement(20632..21699)
                     /locus_tag="PLG98_02225"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOE32096.1"
                     /translation="MTSSKSYKIAVVPGDGTGPEVVAEGLKVLKAVAASESIRYELVH
                     YDLGGERYKKTGEILPDSVLEEFRKFDAIYLGAIGHPDVKPGILEKGILLRMRFELDQ
                     YINLRPVKLYPNVPCPLKDKGPKDIDFVVVRENTEGLYTGGGGFLKKGTVDEVAIQES
                     INTRKGVERCLRYAFQYTAKRNKANKLTLCGKTNVLTYAFDLWERTFNELAKDYPQIK
                     TDYVNVDATCMWMVKNPEWFDVIVTDNMFGDIITDLGAMVQGGMGIAAGGNINPEGVS
                     MFEPIGGSAPKYTGKNIINPLAAICAGGMLLETLGEEKAAKRVEAAVVKLLESGKLKS
                     LDAGKMGMSTSEIGDWLSDNI"
     gene            22038..22979
                     /locus_tag="PLG98_02230"
     CDS             22038..22979
                     /locus_tag="PLG98_02230"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOE32097.1"
                     /translation="MGEGIGDYTKNIVDLRRHMWMAGVEVELRRLIWQIAVVGKYISA
                     KIHESNRKLAGLKNVYGEEQMALDRASDEILKNQLEFSGFVREYASEELENIVKIGTG
                     QEKYIVTADPLDGSSLVDTNLAIGTIIGIHKESVLDCGRNTMVAALYITYGPLITMIY
                     SAGKGAHEFVLNREGEYVLSQENIKLKEKGSIFSPGGLRRDWNPAHLQFIDKLENDGY
                     KLRYSGGFVPDINQVLIKKGGLFTYPALKKSPKGKLRLLFELQPMAFIMEQAGGLAID
                     GDQDILSVKVETINQLSPIYIGSTYEVQLAKEMLSAS"
     gene            23066..24457
                     /locus_tag="PLG98_02235"
     CDS             23066..24457
                     /locus_tag="PLG98_02235"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOE32098.1"
                     /translation="MIYKDKNELQKKYEKIIAIEGNDVSILNETALRGDLIDHLIYTA
                     VFSADQAAQQAACWLIRRAGAALGIVSASIQPLYEAMGREDVRGFTVPAINLRGLTYE
                     SAQAVFRAMMKGKVGPVIFEIARSEIDYTSQRPVEYTAAVIAAAIKTGYRGPVFLQGD
                     HFQINAKKFAADAAKETQTVKNLIWEAIEAGFYNIDIDTSTLVDLSKPTVTEQQQTNF
                     SLAAELTTMIRDLEPEGITISVGGEIGEVGGKNSTVEELEAYMDGYLAELKKQGESLK
                     GISKISVQTGTTHGGVPLADGSVAKVKIDFAVLEKLSAVARSKYGLAGAVQHGASTLP
                     DEAFDRFPAVGTAEIHLATGFQNIIYDSSAFPADLKARIYDHLKTAFQSEWKDKDTEE
                     QFIYKTRKKGFGPFKQDMWHLPADTRDKISAGLEKQFAFLFDKLKMNNTQDVVEKYVQ
                     PVDVAPDVPVSLK"
     gene            24454..25485
                     /locus_tag="PLG98_02240"
     CDS             24454..25485
                     /locus_tag="PLG98_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOE32099.1"
                     /translation="MKAAILEQTTSLKKNKKPLKITEVAEPAVGDGDDVLVKVSVCGV
                     CHTELDEIEGRIFLPSFPVILGHQVVGVVAGQGRRARKFAIGDRVGIGWINRSCGQCP
                     FCLSGNENLCAGFKATGRDVSGGYAQYLTVSEKFAFPIPEIFSDSQAAPLLCAGAIGF
                     RSLRLANLTNGQRLGLTGFGASAHLVLKMARYLYPQTPLFVFARNPAERQFALDLGAV
                     WAGDTSDESPEKLHAVIDTTPVWKPVVEALKNLEPAGRLVINAIRKEDTDKDYLLQLN
                     YGRHLWMEKEIKSVANVALCDIENFLKLASDMRIVPDVQAYPLNDVNQALGELKEGKI
                     RGAKVLIIS"
     gene            25642..27207
                     /locus_tag="PLG98_02245"
     CDS             25642..27207
                     /locus_tag="PLG98_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOE32100.1"
                     /translation="MKIASVGEMRSMDRFAIEHLGIPEDVLMENAGRAAVSVLLSRTG
                     IRGKKFAIFCGSGNNGGDGLVVARLLHSRGGLVRVFLLNDSEKFRGAAATNFEIVKRL
                     PLETAQLTNARPAGRHVAHCDVVIDAILGTGLDRPVSGLVADTISLINKGGKKVLSLD
                     IPSGVNGNTGEIMGAAVTADYTVTFGLPKIGNLLYPGCDVNGELWVSHISFPPSLYDR
                     DDIPVRTNDAMALPPRPADAYKGAMGDVLFIAGAVNYYGAPYLSAMSFLKAGGGYARL
                     AAPRAVIPVIARRGREIVYLPQEETEAGSMALKNKKALLEHSAKADMVVIGPGLSLQD
                     ETVNLVREIAATVRKPMLIDGDGLTAVAGCPDSIRRRKAATILTPHMGEMARLTGKSL
                     KEIHADKIKVLRETVQTLNACIVLKGPHSLIGTRDGNVYVNLSGNAGMATAGSGDVLT
                     GCIAAMFGMGLSFDEATRKGVFLHGVAGDLAALKKGTDGMTAQDIMNALPQALKEDRS
                     GRLQDQYALPDGV"
     gene            27260..28603
                     /locus_tag="PLG98_02250"
     CDS             27260..28603
                     /locus_tag="PLG98_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOE32101.1"
                     /translation="MQINRLTLKFSGELSYLEAPFLKEYYTSSLPHIRMMMILGAMLY
                     AAFGVLDVLLMPQQVVALWIIRFIVIGPVLVGVLAISFLPIFEKYIQPILALVYFLAG
                     AGIIGMIVVAPPPVSYSYYAGLMLVFMWGYTLIRLFFTWASVAGWLQVIFYETVTVGL
                     NPTPLDVFLGNNFFFISANLVGMMACYAIEFYARRDFLMNQQVEEEREKVSRINEELE
                     DRVKQRTEDYKNLNQALLQEVAGHEQAKEKLQEALDSLKKSMGVTIQVMVSAVEARDP
                     YTAGHQIRSSNLACAIAAEMGLPQEKIDGIQMASSIHDIGKLSIPAEILTKPTKLTNL
                     EYSLIKEHSACGYEMLKNVESQWPLAEIIFQHHERMNGSGYPRQLKGDEILLEARILA
                     VSDVVESMASHRPYRPSLGIGAALEEIEKNRGILYDSAVVDACLKLFREKNFYLE"
     gene            28655..30478
                     /locus_tag="PLG98_02255"
     CDS             28655..30478
                     /locus_tag="PLG98_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32102.1"
                     /translation="MTKSFAENRDTKKNLKKYYIMYDPTTVDLMGKIGVAGFTVEDTV
                     EYLTGRKLEACNETNYTEFLSFNTNLLRKGMLEQMAIEPYKAYYIANQICRADQDARV
                     IIADDTRKTIGQIIAEEKQKPVAVFMSLISSTFPTACAVILILNKVNIPVVIGGIHVS
                     TSFNDVDMYLRKYLPHPQIVTQVIGAGDLPTIKNVMSDLARSNLKKEYHGNIPIEDGV
                     WGCDRVIELPKIKPYFINKFPVIGPLLSRIIETNVTTPFLGCPFSCSFCSISSFPEER
                     RKFTSRSPEDFTCELLDKQKKGVTFKNRFYFISPDNLLFGGKKLHDVLDKMIESPLRI
                     NYAAQISIEVADSDKLLQKLRLSGASHFFIGLESLDIRNLEYVGKNIVAKIKKEKISV
                     EEYYASRIKKIQDHGISVHGAFMFGMPYDYFHSLEDHSGRKIVEFCKKNKIGIQPTCL
                     SNLPGSLDFLKGLETHELIYGNPGSMDYFCSLTIADLTESNRKIPDALFNSPLVTFYM
                     LYDTMEKIGSYISALHIAFGMAKKSWSVHSSKGAQSFKDRMVDAFAGIGFQLGASAYF
                     ELYKGLAYSTSSLPGTFERLYQWEKNPTVRKMFKKYVENFV"
     gene            30492..31139
                     /locus_tag="PLG98_02260"
     CDS             30492..31139
                     /locus_tag="PLG98_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE32103.1"
                     /translation="MSKITRSEKEIEEVRNAILEEALTILREEGFEKLSMYQLGRRTN
                     MTAANLYNYFRNKDQLIIAIHKKTFELLYAMLSKAVRKEAEPMAKLKKIIKTFLTFAK
                     KYPNYYDIMFNRRVPQVSDYYGTSEESLAREEYESSARNLNLVMSIGKNVLSSSSEPD
                     VRDVEFYFIRTFSELHGIVSLYNSGILKVIVKNPERAMHRIVDDLLTAYIRRKYR"
     gene            31364..34438
                     /locus_tag="PLG98_02265"
     CDS             31364..34438
                     /locus_tag="PLG98_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOE32104.1"
                     /translation="MQNTLENSSDQNRRSGYWGLAVSASKVGIGKVLRKHQLLLEGFL
                     QFIAGRFNTSEALLEELKGTQGWINLCLGMRSEDSAIEAAIVFQDGVVRVLKTIPENV
                     ETVIIFKTSDHLLDMTDATPDEMYKMMLIGTIRTQGNIMLASLFNYLMALVFDKGQQK
                     AVDRQIEEHRKANKSVGRDVADTDCCRQERQRRKISRVAGGKVDPGVKYLEDPHLAGL
                     GLEDFPRLEQFRAEYFGKKKEAVVCHEYGKLITDFHLANGYEVDKDGKPWDPNLRKAE
                     SLKYILENRTPVIRTNDLLAGTYTTSPVSTCVGHPFSIGCYSWGELRSFSKRELMPYE
                     ISEESIHILHRHVFPYWAKRNIHELWRSRTNGGLPVQIHDRFFSVYYWKTISMSEVPP
                     GHEALIKLGTGGLIRKIEEELARDAHADDEKKNTLKAMIISLEGVNAYARNLARQALE
                     ESKTATNPQRKAELETMHRMLLKIPESPSETLHEAVQNIILMHLCLGMESTDDGPMYG
                     RLDQILQPYFESDMHKLTTPQKREAYIKQVIDILGCLYFIESSHQILAPDIGNWQNGG
                     SSPNGTITLGGVTPQGEDAVNDMTYILLKVTELLSLNNPNVHARYKPDKNSFAYLKRV
                     CDVNYITGATPCIHGDDAVMESLTARGWAVEDVRDWVVNGCVEPGIPGKHCSATSSIE
                     FNLVAVLEMALNNGKHPLMNWKLGPDTGIIGQGDFETFDDFWKAFKEQCEFLCEQSII
                     GNNQLGEIYQQHQPAPLISSMTEGCIESGRGVTRGGARYNSSGVSVIGMADVVDSLMA
                     IKTLVYDEKKITFAELKEAIDSNFAHHPRLHVMIKTRVPRFGSGNEESVTMANRVTSL
                     VHDYFASHQNYRGGPHATGWWSMANHTVYGRVTGASPSGRLAGEPFTPGLTPHPTASP
                     NLLDNLRDVAKLKPETLDNNIAFNVKIVPASSDSHERIIEIMANYADTYFRMGGMQTQ
                     FNVISTETLKDAMAHPEYYSDLMVRISGFVAYFTRLQRDLQLEVIRRAEYKI"
     gene            complement(34442..35416)
                     /locus_tag="PLG98_02270"
     CDS             complement(34442..35416)
                     /locus_tag="PLG98_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE32105.1"
                     /translation="MQIQEIFSIYKEDLQQVEKCISDNAISEIKLIPEVAHHLIDSGG
                     KRFRPLLLLIASRLCGYHGEHRYAMGTVMEYIHTATLLHDDVIDQALIRRGKTSANKV
                     FGNSVSILVGDFLCFKAFMLMSEIGNNNIFELVSRMGNIMSEGEVFQLTKCGDINMTR
                     DDYLNIIEKKTAVLISASCAAGAILGSASPEKIDAMARFGKNAGMAFQITDDILDYAA
                     KGQTFGKSIGKDLEEGKITLPLIFALKQSTDEEKNTMKEILSRKKSSRKAALQVRTFI
                     EKYEGLELSRYTAAGYIKEAKRQLDVFEDCAEKKHLITVADYILSRNV"
     gene            complement(35439..37076)
                     /locus_tag="PLG98_02275"
     CDS             complement(35439..37076)
                     /locus_tag="PLG98_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOE32106.1"
                     /translation="MTTSPDRKETEQAGVSFNTKQTETTSVRPELAEMFARQKFGLDQ
                     NRPEAVEKRRQKNQRTARANVEDLCDAGSFMEYGALAMAAQRGRRSEEDLMARTPADG
                     LIAGIGTINGTFFKEEKARCMVMAYDYTVLAGTQGFFNHKKMDRMLKLAHEQSVPLVL
                     FAEGGGGRPGDVDAIGVMTAGLDLSTFAMFAGLSGKVPLVGIVSGPCFAGNAALLGCC
                     DVIIAAKNSNIGMGGPVMIEGGGLGNFKPEQIGPIDVQTQNGVVDIEVADDVEAVAAA
                     KKYLSYFQGTTPSWEAADPLQLRDLIPENNRRAYNMRTVIKALADTDSYLELRPKFGA
                     GMITGLIRIEGRPFGLIANNCLHMAGAIEAEGADKTVRLMQLCQAHGLPILSLCDTPG
                     FMVGPEIEKRAQVRHVCRMFVVGSHLTVPYFTVVLRRGYGLGAMAMAKGGFHESFFTA
                     SWPTGEFGAMGIEGAIKAGFKKELAAIEDPDQREQLYRDLVAQLYKRGKAVNIASYLE
                     IDAVIDPSETRKWIMRGIKSAATGKKQSPVHNYVDPW"
     gene            37258..37647
                     /locus_tag="PLG98_02280"
     CDS             37258..37647
                     /locus_tag="PLG98_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOE32107.1"
                     /translation="MTANLLKIFNPVKRNKTVLMKKSLSCLQCGKCCFVDFTAYAQQE
                     DYDRWRAENRQDILEMIDHRHLFWAGDRMISSDTGNAPGECPFLYNTGKVWLCSIYET
                     RPLICRDYQPGSSELCPQWKNRKKKEE"
     gene            complement(37658..39016)
                     /gene="rimO"
                     /locus_tag="PLG98_02285"
     CDS             complement(37658..39016)
                     /gene="rimO"
                     /locus_tag="PLG98_02285"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOE32108.1"
                     /translation="MKNAEKTKINIISLGCPKNLIDSEVMGGFLRQSGHALVDKHTDA
                     DIVIINTCAFINPAKEEAIDEILTVAEAKKNRPNLRIIVAGCLAQRYGRELLDQIPEA
                     DRVIGTGEVGNIVRHVTELIRCKNRRKAVITRPVYLMNCNHRRILPASAFSAYLKISE
                     GCSNRCSYCVIPSLRGAARSRPPDDILREAERLAQRGIREIIITAQDTTGYGWNLKGR
                     PRLSDLLNDLTQIKGIEWIRLLYAHPARITADLLSTIAENDKICSYIDLPIQHIDDAI
                     LNAMKRKVNAAEIRQVIAQIRHIIPDVALRTSLITGFPGETPARFNRMLDFVRQTRFD
                     HLGVFTYSREEGTEAAGLRYHVPEKEKERRRETIMHDQMEISASINTSLIGSVQEVII
                     EGKSDRPDYPYIGRCRRQAPEIDGVTYIKGPSPDIGHIVKCRITAADDYDLFGKIIRK
                     SS"
     gene            complement(39013..41268)
                     /locus_tag="PLG98_02290"
     CDS             complement(39013..41268)
                     /locus_tag="PLG98_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOE32109.1"
                     /translation="MEKKQTDNKRTREIKGAVCLALGLFLLLCLVSYHPQDPSFTHFV
                     AGDHTTHNFTGKAGSYTADSLIRLLGAASLLFPLMLFVCSFKYFRSSDFRIGTGRLIG
                     FLFFIFSFAGIMALLISGSISLYGESLKNGTGGLTGVAIVGLLRNYFNVAGTYIILFL
                     IFVVSLTFLVDFSMISFTEKISKSGAAFLLRWKERIMAFVDFVFNRMKSEPPSPPQPA
                     IVEKAPDVTKKAPAKKVEQTHFDFTKVKSDDKFQLPPFTLLEEAPQKDARVKRDALVT
                     NSRILEKKLSDFGVEGRVVEVTPGPVITMYELEPAPGVKINKIVNLADDLALALMAPS
                     IRILAPIPGKSVIGIEIPNLKRESVFLKEVIDNDSFTQSSFRLPIALGVNFVGTPVIA
                     DLTKMPHLLIAGTTGSGKSVALNAMICSILFKAQPEDVKFLMIDPKRLELSSYEGISH
                     LMHPVVTDPKKASQVLRWTVEEMERRYKVLNDLKAKSIDAYNELLLKGLKSKTVLAMP
                     KQIDPELESGQTIDETAREEQEIKHTKLPYIIVVIDELADLMMVAPRDVEESLTRLAQ
                     MARAAGIHLIIATQRPSVDVITGLIKANFPTRISFQVSSKIDSRTIIDQQGAEKLLGS
                     GDMLFIPPGTSKLSRIHGAYVSDKEISRIVDFIKMQAQPAYDDSIEKYALAAAQIQHE
                     GDDDFDAKYDEAVALVSELGQASISLVQRYMKIGYNRAARIIEKMESEGIVGPSDGAK
                     PRKVLIRKLPG"
     gene            complement(41307..41993)
                     /locus_tag="PLG98_02295"
     CDS             complement(41307..41993)
                     /locus_tag="PLG98_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOE32110.1"
                     /translation="MNLLTVIVPGIVQEFTELFPVSRFEHLVIVQSFFPGFHQPGGAF
                     DAVLLLIIAAIPVAVIGYLFKDQMHNIFESARAAAFFLIITGILLFLADKMKKTRRDI
                     KNMTVIDGILSGSARAVPFLTGISRSGTTITSGIFCNLNRNAAARFSFLLSLPAVCGA
                     VILKAGYLRQISSPGSYLYFAGFVCAALAGLLALKLFFLIIREARLKYFAYYGWFFGT
                     FTLLVKSSLF"
     gene            complement(41990..42742)
                     /locus_tag="PLG98_02300"
     CDS             complement(41990..42742)
                     /locus_tag="PLG98_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE32111.1"
                     /translation="MVHSALLVTLGVAITAFMSFWSVVFSVFPAADNKIHKVATLWAK
                     LLLMICNTKVDVIGKENILFGKPQIFMANHQSDFDILISLAHIPVQFRWIAKKELFAI
                     PVFGAAMRSAGYIEIDRSNREKAMQSIDEAALRIRSGKSVMTFPEGTRSREGEIKAFK
                     QGAFHLAVKSGVPIIPISIIGSGRIMPKRSLRIKPGQLKMVIGKPIDVQDVTIENRHS
                     LIEKVRNEIIKNYNAWQDIEMSPLKDLESGAH"
     gene            complement(42882..44714)
                     /locus_tag="PLG98_02305"
     CDS             complement(42882..44714)
                     /locus_tag="PLG98_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32112.1"
                     /translation="MQEIKTANAKKTPPVKSRKDTKEAFLYRPQPEEKHENWLQKSLF
                     LFQHNPASIVLLRLPEKKYIDINKTVLRTFGFSRQDVIGKTSDELDIFVEQDKRQLAL
                     KQLMQQGYIENFEMKFRRRDKTILDGLFSAEIIQHREKRYVLVVITDITSRNLAQQAQ
                     RENEEKYRTILETTEEGYYEIDLAGNFTFFNDPVCALLGYSENELRGMNNRQYTSRET
                     ARKVFQAFNKVYRTGRPTKEFDWEIIRKDGTKRYIEQSASLKKDSSGKPIGFRGIIHD
                     ITERKQMEDALRKSEENFRHSLDDSPLGVRIVTEEGETLYANQAILDIYGYTYVDELK
                     NTPLHKRYTAESYADFQQRQEKRLMGEAGPPEYEISIVRKDGEVRHVLALRKEIFWNG
                     QKRYQIIYQDITERRKAELKLQETLHTLRKSINTTIQVLGIASEARDPYTAGHQRRVA
                     DLARAIATEMKLPQDTIEGIRMAGAIHDIGKISIPSEILCKPTTLSELEFSLIKSHCQ
                     YSYEIIKDVESPWPLADIVYQHHERMDGSGYPRSLKGEDILIEARIIAVADVVEAIIS
                     YRPYRPALGIEIALAEIENNAGTLYDVEAVSACLKLFRNKNFKI"
     gene            complement(44849..>45694)
                     /locus_tag="PLG98_02310"
     CDS             complement(44849..>45694)
                     /locus_tag="PLG98_02310"
                     /inference="COORDINATES: protein motif:HMM:NF027449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE32113.1"
                     /translation="FVKHFLKNRTSAIYDYLDMSPESSLICLREKNETRKAVIRRIEW
                     DKTMLNIKQLRYGTDNFSYLLYGKTQAVAVDGGAWKEILDFLKQNNLMLKIVTNTHSH
                     YDHTPGNDHLLQKTGAEFIRAADFQDHQPLVINAERILVYRTPGHTDDSVCFHAGSFL
                     ITGDTLFNGTVGNCFSGNMNIFFLSIKRLMDLSGETMIYAGHDYILNALAFARHLEPR
                     NKNIDLFRNACRSRNTTYSTLDEERGINPYLRFNEESIIHLLRKNNLPHATEQERWNS
                     LMSLE"
     assembly_gap    45695..45794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            45952..46941
                     /gene="rfaE1"
                     /locus_tag="PLG98_02315"
     CDS             45952..46941
                     /gene="rfaE1"
                     /locus_tag="PLG98_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOE32114.1"
                     /translation="MQKLLTKKRALEIIAGFPRAGVLVIGDVMVDHFIWGNVSRISPE
                     APVPVVDVQKDNILLGGCTNVLNNIHSMGGRAYVSGVVGADTIGKQLLAELNDRKIET
                     KGIVVKKDRPTTLKTRIVAHGQQMVRFDKEDRKPISPVSIHKILDYVRSRVDKIGAIV
                     ISDYNKGVVSKELIQGIKKIVDHSGIFICLDPKQSNFSVYKGVHVITPNHYEVQRAVG
                     MELNGTNNIERLSEIILKKHSFPALLVTRGEEGVSLFENGRKIVHTHFPAQAREVYDV
                     TGAGDTVIGVLALCLAAKASLKEATCLANLAAGIVVEKVGTATVSRQELIDVL"
     gene            46938..47471
                     /locus_tag="PLG98_02320"
     CDS             46938..47471
                     /locus_tag="PLG98_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOE32115.1"
                     /translation="MSKPSPAEPVKLFFSLFAQDAVIIDETIKELSRAYGDPDFISAL
                     QPFDYTDYYGGEMGENLIRRYLTMEKLIHPESLPDIKLATNDIEIKSAAGLKRRINID
                     PGYISKAHLILATGKSYTHRPYLRDGIYADLTLVYQGKKFCVLPWTYPDYADAKQLEM
                     LDRIRNRYLTQLKMTDH"
     gene            47512..48390
                     /locus_tag="PLG98_02325"
     CDS             47512..48390
                     /locus_tag="PLG98_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOE32116.1"
                     /translation="MIKSMTGYGRAEKFFQDKNIIVEAKSVNHRFLEIVLRTPSALFP
                     MEMDYKKKITERFKRGRIEVFIKFEGEGADTAKVNLNMEIARGYFNVLNRLREEFDLE
                     SPVTLKTLTAFRDIFTPPAETEIGSDFLCEVEKSFLEALEILAAMRQGEGLVLLQDMQ
                     MRLKMIDGVLNDIAVRAPQVVSEYQKRLSERIKELTEGFALDENRLAQEVAIMAERTD
                     ITEEIVRLRSHLGQFEVLLHSDESEGRKIDFLLQEMNREINTIGSKVGDVDITRHVID
                     VKSELGKLREQAQNIE"
     gene            48395..48997
                     /gene="gmk"
                     /locus_tag="PLG98_02330"
     CDS             48395..48997
                     /gene="gmk"
                     /locus_tag="PLG98_02330"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOE32117.1"
                     /translation="MSKGLFIVVSAPSGTGKSSICQGFMEAFPEIKFSVSYTSRPPRP
                     GEIDGKDYNFISREAFRKKIRQGEFIEWVENYGHLYGSSRRFIEEGVRDGRDLLLDIE
                     PRGAKKIKQEFRRGIYVFILPPSRQELLKRLKKRGHERENVIQKRYKQSESEIKEISW
                     YDYVIFNNDLKKAVNELIAIYTAEKCKRTRLNREIREFIK"
     gene            49026..49295
                     /gene="rpoZ"
                     /locus_tag="PLG98_02335"
     CDS             49026..49295
                     /gene="rpoZ"
                     /locus_tag="PLG98_02335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOE32118.1"
                     /translation="MARITIEDSLRVAKTRFGLVSLASKRAHQLLKSSQPLVVSKNRE
                     IVQALREIAAGKVVYANPEYLNGTKEDFKPIPDSTEFIGDEEYIE"
     gene            49276..50028
                     /gene="pyrF"
                     /locus_tag="PLG98_02340"
     CDS             49276..50028
                     /gene="pyrF"
                     /locus_tag="PLG98_02340"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOE32119.1"
                     /translation="MKNISSKNAGERLIFALDVQNYEDALSLVDLLSGHVGMFKIGKE
                     LFTAAGPKIIRAIKERNQKVFLDLKFHDIPNTVARAAEAAVKMHVDMFNVHASGGSRM
                     IRESVSASWTCSDALGRARPILLAVTVLTSLNNDDLKEIGFQKTTNELVVHLAGLAKA
                     AGASGVVASAQDIVMLRKAFGEDFVIVTPGIRSAAEIRKDDDQKRTLSAYDAVAAGAD
                     YIVVGRPIRTAADPLKACRQIVQEIAEGLSAR"
     gene            50054..50809
                     /gene="rlmB"
                     /locus_tag="PLG98_02345"
     CDS             50054..50809
                     /gene="rlmB"
                     /locus_tag="PLG98_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOE32120.1"
                     /translation="MEFIYGINSIRDLLRLQKSGWEKIMIASGRTGPAVQDIIAMAEA
                     HNIPVEFCERKKLDKLAGTPNNQGMIAFRQTFAYADLETVMKNRHPACGHDLILILDS
                     VMDPQNLGSIIRAAHCWGANGVIIPEDRSAGVTAAVNKASAGSVHHIPVAKVVNLAQL
                     LDHLKDRGFWIFGAETHGGKKLTETDFDCPVALVMGGEAKGIRPLLKKKCDFLTTIPL
                     TGNFDSLNVSVAAGIILYEISRQRTRSTKQSGK"
     gene            50980..52188
                     /locus_tag="PLG98_02350"
     CDS             50980..52188
                     /locus_tag="PLG98_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE32121.1"
                     /translation="MPVFIWEAVTKKDEAKKGEMEAVDEITVRSLLRRQGYKSIEVKR
                     KPKDLLEYLPFLQGKVKEKNVVIFCRIFSTMINAGLPLIQCLDLLAQQEQNKAFSKII
                     KSIKEDIEGGTSLTNALKKYPEIFDELFVNLIAAGEAGGILDIILQRLSSYMEKAMKL
                     KARVKGAMTYPAAVLVISCAVVALLLIKVIPVFQKMFEGMGGELPALTAALIAASQFA
                     QSYWYLGVAILVVIYIILSRFYRTEKGRWTIDSLILKSPVFGPLLKKVAVAKFARTLS
                     TMMSSGVPILEGLSIVSKTSGNVVIEDALMKTRQGISEGQSIAEPLTKTGIFPPMVVQ
                     MIAVGEATGALDSMLSKIADFYDDEVDAAVDAMTALLEPVMMVFLGGVVGGMIVAMYL
                     PIFKLASVVG"
BASE COUNT        13407 a        12068 c        13750 g        13197 t
ORIGIN      
        1 acggacatgc tggttaacag ccagccgctc taccgactga gctactgggg aacgtatttt
       61 caaagattcc gaatttttgc cccgggtatt gatccgacat gacggattgc cgaagcattt
      121 ttttctggcg cttcataata caagaagaga cagatgtcaa catcaattaa atatttattc
      181 aaaatgataa aaaaccggat ctttatatga aaaatgccga attcttttgc aatgttcgga
      241 aaattgaccg ccgaaatgtt tcatcaggcc gtatcatttc cttgcctcaa aaatcacgat
      301 aggatataaa agcctacttt tatgaatcag aacggcaaat aaggttggca atgatgacag
      361 gcagccagaa attcttgaaa aaacatgttt tggacgaggg gttatgcacc ggttgcggcg
      421 cctgcgtggg catttgtccg tatcaggtga tttatcatga ccgaacggtg caattacacg
      481 gctgcgattt ggtaaaaggc cgttgttatg attattgtcc ccggacgccc tgtgatgtcg
      541 gagccatcag ggaacgatat tgcgcagccg gtgatctgac gccggaaatc ggcgcggtca
      601 agggatatta tttttccaga gctgctgatc ctgggttacg aaacatcggc cagcatggag
      661 caacagtaac ggctatgctg gagttggccc tgtctgaagg cctgattgat gcggctgttg
      721 tttcttcccg ggaccaatta tcgctgcgaa aaggcgttat tgtacaaaac agggaagcga
      781 tcagaacaca tgccggcagt caatttaccg tatcggcgac ggtagccgct tttcaccaac
      841 tggtgacgaa gcacaccggt caagtcggaa ttgtcgccac gccgtgtcag gcgctggccc
      901 tggccaagat gaaattcaat ccgggacatg aagacattgc cagagtcaat caactgacgc
      961 tggtaatcgg tttgtactgc ggctggacgc tttccgaaga aaaatattta aagttactga
     1021 cggataaagg catcgctttg gaatccataa ccgaaatgga catccccgcc ggcaggaacg
     1081 ttctggagat ccggacggaa gatcggctgc attcgattgc gatggaggaa gcgcaaacct
     1141 gtatccggga agcttgtctg tattgtatgg acagcacggc ggaattcgcc gatgtgtcgg
     1201 tcggttcggc gcggttttcg ggaaattggg aagacgttcg ccagtggaat cagttgatcg
     1261 tcagaacgac aaaagggaaa gaactgacgg atctggccgt tgagaagggc gttctggaat
     1321 tgcgggaggc gcccgccgac agtttaaaag aactgaagga agcggcggtc aataagaaga
     1381 aaaatgcgtt gaaaaatatt atccaaaaga gcggctcggc aaaaaatctg ttatacttgg
     1441 acagtcgcga tccggcggtg ctgaaatatt tggaagctga aaagaaaaaa agtaaaaagt
     1501 gaatggtgag aagtgaatat ttgtgcctct gttcagccaa gtctttttga cttggctgtc
     1561 aataaaaaaa tcagtcgggt ccaaaagact cgactgagct tgaggggggg gaacggcggg
     1621 gtaaacaaga gaaaatcaat gaaatgtcaa gaataatgaa atgatattac accctcggca
     1681 tgacaacttt tatgaaatat attcataaga ttttacgttt gattttattt tctgtttcca
     1741 tatttatgtt tgcctcgagc tctgcttttt gctccgtggc ttcagggcgg ttcagtatcg
     1801 gtaatatgag tgaggacatt gcgatattaa tagcctatgt tcttctggct ttattggttt
     1861 catttctatg ttcaatagcg gaagcggtct tattaagtat tacgccttcg ttcattgagg
     1921 aacagaaaga aaaaaatccc aagcgggcca ttctgttgaa gcgactgaaa caggataatg
     1981 tggatcgcgc gctggcggcg attttaacac tgaacaccat cgcgcatacg gtgggcgcca
     2041 tcggggcggg agcaaaagct acggtcgtgt ttggaagcgc atggtttgga atcttttcag
     2101 cggttatgac attaatgatc ctttttcttt cagaaatcgt tcctaaaacc attggcgcca
     2161 tttactggcc caaactcgtt gtgcccacgg tattttacgt acaaaccctc attgtaacgc
     2221 tgtttccgat tgtttggatt tcagaaagga tgacaaaatt tatttcgcgc ggaaagaatc
     2281 tgcacatttt cagcagagac gagtttgttg ccatggcaag agtgggcgaa caatccggac
     2341 agatagacga taacgaggcg cggattatca agaatctctt ccggttcggg tcgctcaacg
     2401 taacggatat catgactcca cgcacggtga tcgcggcact ttccgaggat aaaacggtag
     2461 ccgacgcggc ggaatatgtt gtaaaaatac cattttcacg aattgtgata tataagaagg
     2521 atatagacga tataaccggg tttgttttga gagatgatgt gctgctgcgg gataatcagg
     2581 aaagaggtgg cgaggcgctc aagaccttaa agagagaaat attggttgtt cccgagtctg
     2641 cagtacttac ggatttactg gaaatttttc taaaagaacg tcagcacatc gccattatag
     2701 tcgatgaata cggaggaaca acagggcttg ttacgctcga agatctgatc gaaacattaa
     2761 tgggtttgga aattgttgat gaaatggaca cggttgtgga tatgcgtgtt ttggccagaa
     2821 aaaaatggga agaacgggca ggggcgctcg gcataaaaat taacgtggat gggaaagatc
     2881 aatccaaaaa agaaaatgtg ctaccggaat aataaagaag ccgactctgt ataataagaa
     2941 actaaacttc cgcgcagcgt cggaaatgat agaaatactt ttaagtgaat tataaaatga
     3001 taaataaatt attatcaccg gccaaaacag aagtgttgtt catgggcaac gaagccatcg
     3061 cgcgcggggc tctggaggcc ggcgtaagtg tcgtttccgg ttatcccggc acgccgtctt
     3121 cggaagtgat cgagcgcctg tccgaagtgg cccgtgaaag aaatctctac gtggagtggt
     3181 ccaccaatga aaaggtggcg ctggaagtgg ccgccgccgc atcgttttcc ggtttgcgtt
     3241 ccatgtgcgt gatgaagcaa aacggcgtca acgttgcctc cgattttctt ctgcatctgg
     3301 cctcgtcggg aacacgcggc ggcatcgtgc tggtcgaatg tgaagacccc ggcgcactct
     3361 ccagtgtcaa cgagggtgaa tcgcgctatt tcgcgcgcat gctcgaaatt cctatgctgg
     3421 agcccgccaa ttttcaggaa gccaaggacc tgacccggtg ggcctttgag ttatccgaga
     3481 aaatccgaaa tatcgtcatg gtgcgctcgg ttacgcgtat gtcgcacgcc agcggtaatg
     3541 tcatctgcgg cagtctgccg gctaaattcc gcaacgcccg ctttatttcc gacggatcgg
     3601 tcatggatcc tctgcgcggg ccggtcgtca gcacgccggt ggttattaaa cacggcctgc
     3661 aacagaaaaa attacagaca gcagcggagc tttttgaaaa gagtcctttc aacagctacg
     3721 ttggtcctaa aaaaccggag cttttgatca tcaccagcag cgcctgcttc ctatacagcc
     3781 gggaggctct tgaaatactg ggcttgcaaa aacgtgtcgg aattttgaaa ttgagctgta
     3841 cctggccgtt gccggaaaag cttttgaaaa aatatctgcg cctgaccaaa aagattatga
     3901 ttgtggaaga ggtactgccg tttctggagg aaaacatcaa agtagccgcg atgaccatgc
     3961 tgaaagatat aggcatcaaa acgttttacg ggaaaacgga caagaccctg ccttcagccg
     4021 gcgaattgaa tcccgaaata gttatgcggg ctctgagcaa aatcatgaag gttaaaccca
     4081 agtccttgcc tgccggttat ctcaaggaag ttcaaaaagt ggcgcccaag attgctccgc
     4141 gggcgcaaac gttctgtccc ggctgtccgc accgtgcgtc tttctggaac atcaacacgg
     4201 cgctgaaact cgacggccgc gaaggcattg tctgcgggga tatcggctgc tatgcgatgg
     4261 cctcgctctg gccggaaatc ggtttccata ccgtgcgcac gctgcattcc atggggtcgg
     4321 gcacggggct cgccagcggc tttgcgaagc tttcggcttt cggtctggac aagcccgtta
     4381 tggcggtttg cggcgattcc acgttttatc atgccgttat tccggcgctg atcaacgccc
     4441 aacataacca ggccgatatc acctttatcg tgctggacaa cgcgggaacg gcgatgaccg
     4501 ggttccagcc gcatcccggt attaatgaaa gcgcgatagg cgaaccactg cctcccgtgg
     4561 atattgaagc ggtctgccgc gcaataggcg caaaggttaa agtgagcgat ccgtttgatc
     4621 tggaaggaac aagcagaata ctgacatcgt tcatggccga gcgcggctcc atgaaagtgt
     4681 taatcatgaa acaggcttgc gctttgagcc ctgaaaagaa gggcaagaaa cagtaccaga
     4741 tgaaagtcaa tgaagatctt tgcctgggcg atcaatgcgg ctgcaaccgt ttgtgcacta
     4801 gaattttccg ctgtccggcg ctgacctggg accggaaaaa gcaaaaagcc gtgattgacg
     4861 acgtcatctg cgccggctgc ggcgtctgct actccatctg cccgcaaaag gcgattgcca
     4921 gagtggaggc tgaagcatga aaaagtctct taagaaaaat ccaacaaaca ttattatcac
     4981 cggtgtcggc gggcagggaa acgtcatggc ctcgcgcgtg ctggccggaa tgctggtgaa
     5041 cgcgggcttt atcgtcacca tcggtgaaac cttcggcatg tcccagcgcg gcggctcggt
     5101 aatgagccat ctgcgcgtgt cgtcatcgtc ggttctgagt ccgcagattc cgcagggcag
     5161 gggggacatt gtgattgcgc tggagccggt ggaggcgctc agagtactca ccaaatatgg
     5221 taatcccgat gtagcggtgc tgaccaattc acgcatggtt tatcccatgg gcgtgatcac
     5281 cggcgaattt acttatccga cgattgaaga agtcaaatcg atgtttgaaa aaatatcggc
     5341 aaaaaactgg atcatcgacg ccacgtccgt tgcggtggaa ttgggaaatc cggttttgag
     5401 caatatcgtg atgatcggcg cgctggcagg tacgtcactg ttgccgattg accgccgggc
     5461 gtttgaaaag gaaatcgcca aaaccattcc agccggtaaa catcaaatca atctgaaagc
     5521 ttttgatgcc ggggtaaaaa tgctctaacg taatgtaggg ctgtagaagg ggtgcaagga
     5581 tggacgctga ccacggttct tcaggcaaag taacatacaa ttacaggttt atattcaaag
     5641 acggcgtgga aaaagaattt aacatcgcgt tggacagcgt cactttgaat ttactgccga
     5701 cggataaaaa aaattatccg gaatggacaa agctgaattg tttcaggtgt tctcattgta
     5761 cgttaaacga tcaatgcgaa tattgtccca tagccatcaa tatggtggat gtcgtagatt
     5821 atttccgcaa ttatttttct tacgatatcg tggagacaat tgttacggca caggaaagaa
     5881 aatattttcg tacggtgtct ttgcagcacg gcgtcagttc tttgatcgga atatatatgg
     5941 ttaccagcgg atgcccgttg atggaaacat taaagcccat ggtgcgtttt cacctgccgt
     6001 ttgccacaat agaggaaacc aaatatcggg cgatttcaat gtatttgatg gctcagtatt
     6061 ttctgtatca gcatggacgt cagcccgact gggatttaaa gaaactggcg gaagcgtatg
     6121 agaatgtgcg aatcgttaac gaatactttt gcaaaaggct cagaatgatc caggggcagg
     6181 acgccagtct gaatgctgtc gtgctgctgg atattttcgc cgacagcgtc aatttctcga
     6241 tggacggacg gatggccaat gatcttgagt atttgtttaa aggatatttt gaatcgacgc
     6301 cgggtgccaa taacgcttcc tgaattacct tgaattgtct acaaaatttt aaaagcgttt
     6361 ccgctctaaa aacaaccgtt cagggtttgt ttttttcagg attcgccggg tgacttcttt
     6421 tgcgtataat cgtaaaatcc cttgcctgtt ttcattccca gctcgccccg gtccacgtat
     6481 ttcttcagca ggtcggcgct taattttgcc tggtgattat tgcgtttttt cgcccagaaa
     6541 tcggtgattc gccatacggt ttccagaccc acgtaatcca tcatctcaaa gggacccagc
     6601 ggtgaagcgg tgatttcctt ccaggaacgg tccacgtctt cgggagacgc gatgtcccgc
     6661 gaagccagat tcaacgccgc caaaaaccag ggattgagaa gcgagttgaa cacgtacccg
     6721 gctttttcct gcttaaggac aatcggcgaa tgtccgatta attcgctgaa acgctggacg
     6781 gtttcaaccg tttccggcga tgtgcccgga tgtcccatca catccaccag cttaaaaccg
     6841 ggatgaaaat gccaggcgag aaatttttcg ggccggccgg ttttatcggc aaacatcgac
     6901 ggcaccagag aagaggcgtt tgtggtaaag atggtccgcg ccgggcagac cttgttgagt
     6961 tctgcaaaaa aatctccctt gatgtcggga tcttcaggaa tcgattcgct gaccacatcg
     7021 gcatcggccc cggcggcagc aagatcggtt gtcgccgtga tgttgttctt aaacgattcg
     7081 gccttgtcct tcggcatcat cccgaagtcc gccattttat caatcttttt gttcatgatg
     7141 tccctggcct ttgccgggat gtcatcgttg atgtcgtaca ggacgatggc atagccgctt
     7201 gtgacgcagg aaagaccgat gctccggccc atggttcccg ctccggcaat gagtattttt
     7261 ttaatatctt cagctttcat ttttccccgc tttccgaaat agctgcaaaa aaaaatctat
     7321 ggtcggagtc ggatgaccga ttttcagaat gaaccaaacc aaacatttat ctgctttatt
     7381 tcgtgtaaaa ccgttttatt tatccggcgc aaaataatgc tttttaccgg tcaagtcaac
     7441 agatcgttta tgaaaattcc agaaatcccg gacaataata tttaaaaggg gctgctttca
     7501 atagtgttaa tttcccgaat aatattgtat gccttcagtg gctgtgatca aaaaacgggt
     7561 gaactatata tgcgcatatg atagaagaaa tccaaacagg tccccatgtc agataagata
     7621 aacatcggtc catccggacg taatattatt ttctgtatgg ttttgacggc gttgattctt
     7681 gctgcttacc tgcaggtgca tcaatttgat ttcgtcaata ttgatgacag tgtctacgtg
     7741 actgaaaacg attacatccg gtccggcctg acgcttgatg gaatccgctg ggcctttacc
     7801 accttgtacg ccgaattctg gcatcccctg acgtggcttt cgctgatgct cgattatgaa
     7861 ttatacggct tacatgcagg cggctatcat ctcacgaatc tttttcttca tatcatcagc
     7921 tccttgctgc tgtttgggat gtttcagcgg atgaccggcg ctttctggcc gagcgccttt
     7981 gtcgccgctt ttttctcttt gcatccgctg cgggtggaat ccgttgcctg ggtagccgaa
     8041 cgtaaagatg tattaagcgt ttttttctgg atgctgacgt tatattttta tgctctgtac
     8101 acggaaaaac cggtattcag aagatatctg gcggccgtct tcttttttgc ctgctcactg
     8161 atgagcaaac cgctgacggt gaccctgccg gtcatcatga tgcttctgga ttactggccg
     8221 ctgaaacgtt ttgcatcacg cagacaaaat ctgtttttat ggcagttgaa agagaaactg
     8281 cctttcttcc ttctttcggc ggttttctcc cttatcgcca tttacgccca gccgaaattt
     8341 cctatcgacg gttggccgtt ttcgctggaa tcaagaatca tcaacgcgat tatttcattc
     8401 gttctttatc tgcagaaatt gttctggccg tatgatctgg ccatttgcta tccgtttttc
     8461 ggccgggctc ccgccggaca aatcattgcg gcggttttac tgtttttcgc cgtcagcgcg
     8521 gccgtcgttt ggatgatgaa aaaatcaccg tgtcttttgg tcgggtggct ctggtttatc
     8581 atcaccatac ttccagtcat cggcattatt cccgcgggaa acaatgcgat ggcggaccgt
     8641 tatctctatc tgccgtccat cggcattgca gtcatgattt ttttcgggat tcctctgctg
     8701 gttgaaaatg aaaagatcaa aaagtttttt ttgccgcccg ccgctttgat tttttttgct
     8761 gttctgtccg ggatgacctg gcaccagtgc ggtttctgga aaaacagtga agcactgttc
     8821 agaaacgcct cgcggatcaa tgaggaaaac gccctggcgc atatcaatta cggcaatgcg
     8881 ctggccggtg agggcaggct tgaagaggcg gttgtctatt acaacaaagc ctggggaatg
     8941 cttcctgaga caggcgggaa cgtcatggcg tataataaaa tgggtattgc ggaaaataaa
     9001 ctccgccagc ttattgatgt cagtctgggc aacgtctata caaagcagaa aaaatatcag
     9061 gaggctgtgc atcactatac cagggcgatt gccatgcgtc ccataacgcc ggatcatgtg
     9121 cggacctata atttcagggg gacggcttac agccgcctgg gacggaagaa agacgccatc
     9181 agagatttta atgaagccat ccgtctggac cgacgtgatt acagggctta caacaacaga
     9241 ggattactgt acgctgaaac aggtatgcac cggcaggcgc tgcaggattt caatcaggcc
     9301 gttttgatga acccgaataa cgcggagctg tataagaaca gagggattgc ctgtgcgagc
     9361 ctcaaacttt atcagaatgc ttttgaagat tttgaaaaag cactgacact ggtccctgat
     9421 gatgccaata tttattataa cagaggcgtt gcttacatcg aactgggccg tcatcaggag
     9481 gctttctcgg attttaatga agccattcga ctgaaagcgg attatacgaa tgcctaccat
     9541 taccgaggcg gaatttacct gatgtatggt tatgctgcat caggatgtga tgatgcccgc
     9601 aaggcctgcc aactgggcaa ttgcgaaata ctggaggcag caggaagcaa aaacttatgc
     9661 cggtgattgg ttccggtggg tcttcgttct gtaaaaaatc ttttcttaaa aagccggtca
     9721 gagggatgaa tgacggctgt ctcacatctt cagaaacagg cgccatcccg cataaagcga
     9781 acccggaatg aaatccagcg ccccggacgc ctcagcaacg ggcgtgaatc cgcaggcatc
     9841 gcagttaatg ttgccgcggt ttttgacgta gcagcctttc gtgatggtgc cgtccggatc
     9901 gacgttgatc agaatatcat catgacagga ccagcggttg tcgatcattg ctcggagtcg
     9961 ccccgcggag tttaatatgg gcaacccgga tttcttcagt tgcagcagtt tttcgatggc
    10021 ggcgcggcgt tcttccggtg gcagcgagag atcgtcttct ccctgatggt aagggtaaaa
    10081 gaactgaacg gtcatccctt ttaatgcggg tgtgtcctga acccattgtg ctacgaacgg
    10141 cagatcctgc cagttgcggc ggttcagggt gtaatgaata aaaacacgcg ggtgacgggt
    10201 gttttgaata ttggcgcgga cttgatcgaa ggaattgctt ctcaatgtat tatgcgtctc
    10261 cctttgaccg tccaaactta cccagagggt atgggacggc acatccagag gcatggtgcc
    10321 gttggtggtc accgctacac ggaggaattt ttttctggcg taaagaataa gatcgtgaag
    10381 acgataagaa ccgtcccgcc acagcagcgg ctctccgcct tcgaaaacaa tcatccgcgt
    10441 gccgagctga ttgagcagat ccattatgtg cacggcggca tcccgcgaca tatgttcttt
    10501 ttcgcgatcg gaacgcagat gaaaaggaca tgcccggcag ctcaggttgc agcgataggt
    10561 gatcttgaag ctggccagca aaggcagttt tcgcttcagc atttttcgtt gaatataaaa
    10621 ccacgcaaga tcaagcagcc agggcgatga atggatgtcc ggatttctga tgtctttgcg
    10681 caaaatggta tcacgtgtct ccggtattca ggaaaatatc agccggcaaa ataaaagttc
    10741 aagcagcttt tttccggttg aaagatgacg aaattttcct ttagaaacta taggaataaa
    10801 cgagaggcat gtcaatacga ttcgcgatca tgccggttgg cttgcgctgg ccggaatgat
    10861 gaaaaaaata atggcggcgc gaaaaaaatc tatattattc aggctttgtt gccttttgca
    10921 atttctgcca atgcatcgaa taaaaaatac cgtcgatttt atataagtat tgattgacat
    10981 gaccggatga gaccttatat tattctcaac ggaaattcat caagaggtgg gaccatgaac
    11041 gatatttatt cggctgttaa agtgacagat catgtttact gggttggggc gatcgattgg
    11101 accatccggg attttcacgg ctacaccacg ccgcgcggca gcacatacaa tgcttatctg
    11161 gtgatggcgg atgagattac cctgatcgac accgttaaaa agccttttat ggatgaaatg
    11221 cttgcccgga tcaaatccgt cgtcgatcca tccaaaataa aatatattgt gtccaatcat
    11281 tctgaactgg atcataccgg ttctctgccg gacgtgattg atttcataaa accccaaaag
    11341 gtttttgcgt ccggggtggg gacaaaaaca ttaaaagatc tttttcatga cgagcgggaa
    11401 attattgctg tcgaaaacgg acagtctctg aatctgggca atatgaatct gacctttatg
    11461 gaaacgcgca tgattcactg gccggacagt atgtttacgt atctggatgt cgatgaactg
    11521 ctgttttcgc aggacgcttt cggcatgcat ctggcgacgc tggagcgttt tgccgatgaa
    11581 attcccgttt cgaccctcga atatgaagga gcgacatact acgccaatat tgttctgccg
    11641 tattctccta ttgtgttgaa agcgctggag aaggtcgcgg catcgggatt gaaaatcagc
    11701 gtgatcgctc ccgatcacgg accgatctgg cgtaaggata tcggttggat tatcgattta
    11761 tataagaaat gggctttgca gaaaccgaca gccaaggccg tggtgattta tgcgaccatg
    11821 tggcactcta ccgaaaaaat ggcgaggatc atcagcgaag cgctggctca ggccggtgtc
    11881 aaggtcagcc ttatttccat gaacgaaacg caccgcagtg aagcggttta tgaagtgctt
    11941 gatgccggtg cgctcattgt cggctcgccg acactcaaca acaacattct gccccagatg
    12001 gcggatgtga tgacttatct gaagggtttg aaaccggtca ataagatcgg cgcggcgttc
    12061 ggatctttcg gctggagcgg agaatctgtg aaacatctgg aggccatgct caaagagatg
    12121 aaggttgata ttgcgtccga gtccgttgcg gtgaaacatg ttcccggcgc cgacgaattg
    12181 gatcaatgtc aaaaactggg caaaacggtg gcggcaaaac tgctcgagaa ggtgaatagc
    12241 cagtaaaata tcgaaatata taaaggagga tgtgaagatg aaaaagtacg tttgcggtgt
    12301 atgcggttac atatacgatc ctgctgaagg ggattcagaa ggcaatattc ctgccggtac
    12361 tccttttgaa aaattgccgg aaaactgggt ctgtccggtg tgcggagcgg ccaaagacga
    12421 attctcgccg gaataatggt cccggtaaaa tgatattgcg agccttttct cagaaggctt
    12481 tgcaatcttg aaacaagact tttccccggc atggctggac tataacttgc cggttgaagt
    12541 ggcaaaaaat aaaaggttgg taaaacgtat ggatataaag gaagcgttga gtcaggcata
    12601 taccggagag gcgaaggcgg ctctccggct taaggtttac gcgcagaagg ctgaggatga
    12661 aggctacaaa caaatggcga agctttttcg ggttattgct ttttcggaag agattcacgg
    12721 cgcccgggct ctgaaaggtc ttaaggaaat aaaaagcacg gaagaaaatc ttgcggccag
    12781 ttttgaatcg gaaacgcagg ttgccgaagt tgcctacgac cggtttgtga aactggcgga
    12841 agccgaaggc aacaaggagg ccgtcctgca tttcagccag agcaaggatg tggaagaaac
    12901 gcacgccaaa ctttacaagc aggccatgaa ccattttatg gaagaacgcg aaaccgatta
    12961 ttttgtctgt aaaatttgcg gatatgtcgc cgacggcgtt ttaccggagg aatgtccggt
    13021 ctgcggcgcg aaggcgaaaa tgttcgtggc gtttcaggga tgaggttttt tgaagcgggt
    13081 ttgcgatatt gagcaaccgc ccggggtatt tgacgggcgc aacctgatga atagggcaaa
    13141 tacgtgcaca ttcgatatac cgtcacggtt tttttattga tcatttattt ttcacccggg
    13201 aaagccattg gcgaagaaaa gccgctatgg gagattggtg cgggaatggc ttttctccag
    13261 atgcccgatt atcggggggc ggatgaaagc agattttatc cgctgccgta tcctcatgtt
    13321 gtctatcgcg gcgatttttt taaagtcgat gagaaccgga ttaccggaca aatttttaaa
    13381 acggacaggg tcttgctgga tttcagtatt ttcggctccg taccggtcaa cagtgacgac
    13441 aatgccgccc gcgaggggat ggatgatctg gatccgacgt ttgaattggg accggctttg
    13501 aagattaagc tgtgggaaca caagcaacac aaaacccgaa tgaatctgac gctgcccgcc
    13561 cgtgcttttt tttcgacgga tttttcttcc gtgcgtcacg agggatgggt cttcagcccg
    13621 cggatcaaca ttgtgacgga tgatttaatt ccccgtaccg gtttaaatct gggcgtttct
    13681 gccggaccga tgtttgccga cagcggttat cacgaatact tttatgaagt ggaaccccgt
    13741 gatgccaggg tgactcgtcc cgtgtatcgc gctgacggcg ggtacagcgg ttcaacggtg
    13801 acaatcggtt tgagcaaaac ctataaacac tttatctttc atgcttttgt cagcgccgat
    13861 tttcttcagg gcgcttcttt tgaagacagt ccgctggtga aaagggaaac gtccatgatg
    13921 agcggtttca acgtttcctg gatatttttt aaatcggaaa agatggtaaa cgcggaaaaa
    13981 tagccctccg tgaaaaaggg ttgaacgcgt tgcctgattt cagatattga tgggtcctga
    14041 tatgattatg attattacaa gatggctgat catcacggcg gcgattctgc tggcttcgca
    14101 attcgtgccg gggatcgaag tcgataaact ttccaccgct gtcattgccg cctgcgtcct
    14161 ggggctgatc aacattttta tcagaccgat tgtggtgatc ctgaccctgc cgttgagtat
    14221 tctgacgctg ggggtgtttt atttttttat caacgctttt ttgctggaac tggttgctta
    14281 ttttgtatcg ggatttgcga ttaaaaactt tttttccgca tttgccggct cgctgattat
    14341 cagcatcgtc agctggctgg ccaattcatt tatcacctcg cataaaatcg tcaaaaagga
    14401 cgatccggac tacattgatc tgcggaaagg ggacgacggt aaatggcggt aggcgcgacg
    14461 ctcgatttga ctccggcgat gaaacagtat gtcaaaatca agcaacagca tcctgattgc
    14521 attctgctct accggatggg cgatttttac gagatgttct tcgaggacgc ggtgacggcc
    14581 gcgcccgttc tggagatcac gctcacgtcg cgcaataagg gcaaagagga cagcgtgccg
    14641 ctttgcggtt tcccgtatca tgctgcgtcc gcctacatca ccaagcttgt ggaaaaggga
    14701 ttcaaagtgg cgatatgcga gcaggtcgag gatcccaaaa aggccaaagg gattgtcaaa
    14761 cgggaggtta tccggatcat tactcccgga ctggtcgtgg atgaagaaaa tctggctccc
    14821 ggtgaaaata attatctggc ctgcctctgc gccgaaaaaa acaccctggg tctggctttt
    14881 ctggatattt caaccggcga attccagatc ggtgaattca cggaccgcga ttatttcttc
    14941 acggtaattt ccggcctgga ttttaaggaa ttgctcctgc cgcgaaattt ccccgacaga
    15001 attttactcg acaccatcga agcacagatg cctcaattgc gcatcaacga tctggacgaa
    15061 aactattttc tgaccggcca ggccggggat gttctacagc ggacgttttc aacggacgtt
    15121 ctggatgcgt gcggcatcag cgaacatccg gcggcattga aggcgtccgg tgccatattg
    15181 agctacgtca acgagacgca aaagatcaat ccgcagcaca tcagcgcgat cagatggtac
    15241 tccgcggaaa agtttcttct gttggatgat accgcccgcc gcaatctgga aatcttcacg
    15301 tcgatccaga acaatacggc ggccgggtcg cttttttctc ttttcagtga aacgcaaacg
    15361 ccgatgggga cacgccgttt gcgctggtgg atgagctatc cgctggtgga tgcagaaaaa
    15421 attaaaaccc ggctggccgc agtggcggag ctgaaagatg atcctcttcg gcgggcgaat
    15481 ctgcgaaaaa cattgtcgcg tatatatgat ctggagaggc tggccggaag agtgtcgctc
    15541 aaagcggcca atgcccgcga tctggtcgcc ctgaaaaatt cgcttcttgc cgttccggag
    15601 atcaagtccc tgctcagcga ttgcacggcg ccggccattt gcgccatccg gtcgcacatc
    15661 acggagatga ccgatgttgt ggatgtgatt tcacgggcga ttgtcgacag tccgccgcag
    15721 aaaattcagg acggcggatt tatcgctgca gggtatgatg aagaacttga taagttgcga
    15781 ttgatcagcc gcgacggcaa gcaatggatt gctgctctgg aggcggaaga acgcaagaaa
    15841 acaggcatca attccctgaa agtgggcttt aacaatgtgt tcggttatta cattgaagtc
    15901 accaaagcca acgcagctct ggttcccgac tcttatatcc gcaaacagac cctggtcaac
    15961 gcggaacggt atatcaatca ggcattgaaa gaattcgaac agacggttct caacgcggag
    16021 gaaagaatca agcagaagga gcaggaactt ttcatcaatc tgcgtgacgg acttgaccgg
    16081 tatatcgcgg agattcaagg caatgcaaat ctgattgccg atcttgacgc cctggctgcg
    16141 ctggcggacg tggccgaaaa atatcattac gtctgcccgg aaatcgatga tgaggagacc
    16201 atcgacattc aggacggacg ccaccccgtt gtggagtcgg ctttgaaaaa cgaagggttt
    16261 gtgccgaacg actgtttaat ggatttgcgc cagaataaac tgctcgtcat caccgggccg
    16321 aatatggcgg gtaaatccac gtatatcagg caggtggcct tgattgttct gcttgcccag
    16381 atgggaagtt tcgtcccggc ggccaaagca aaaatcggaa ttgttgataa aatatttaca
    16441 cgtatcggcg cctccgacag cctgatcaag ggacagagca cgttcatggt ggaaatgacg
    16501 gaaacggcgg aaatcctgaa aaacgctaca ccccggtcgc tggtcatcat cgatgaagtc
    16561 ggtcgcggaa cctccacctt cgacggattg agcattgcct gggcggttgc cgaatacatt
    16621 catgatttcc agggacgcgg tgtccgcgct ctctttgcca cgcattatca tcaactgacc
    16681 gatctggtgg taacaaaacc cggggcgaaa aattataata tcgccgtgaa ggaatgggga
    16741 gaaaaaataa tttttctgcg caaaattatg gaaggcggga ccagccgcag ttacgggatc
    16801 gaagtggcgc gcattgcggg cgtgccggcc gacgtcatta tgcgcgcaaa ggaaatattg
    16861 cggaatctgg aaaaagggga attcgacgag atcggcatgc cccgcatagt ccggggagcc
    16921 cgcgaaggca aaaacgccaa tccgcaattg agtctttttt caagacggga aaccgccatt
    16981 gctctcgaaa agaaaggaca gaacctcatc gaggcactga agaatatcga tatttcctcc
    17041 ctgtcgcctc tcgaagcgct caataaattg aacgaactga agaataaaat ttctgattaa
    17101 atcatttacg attcatgagg aaagctttca atcctccatc gatctttata ttccttggcg
    17161 ctcctggcac tctttgattt ttttaagagc ttgtttatta acaaaatact ctgaaaaggt
    17221 ttattcaaga aaaagcggga ggcttaatag cctcccgctt ttgctgagag ttgaaaccta
    17281 ggagaagact taaagggttt catggtttgc gtataatttt tatgtgcaat tgatgtgcca
    17341 tagcttgctg attttttaat atattgaaat tacaagcatt attgaaatgt tttaaaaaat
    17401 agattgtgtt ttataaaata ttatggataa taatgatcca atttacgtat ccaatttagc
    17461 attcatctgg ataataatta tccagatggt ttcatttttt aaaaaagaaa tgaggtccgt
    17521 ctcatttttt tataaaggat gatgaaacaa agaaccaggg gtaataatca tggttgttga
    17581 aaaagctgtg ctgattgttg acgatgattc cgttcaccgg atgatactca aaaaacattt
    17641 gaacgactgg gggtatcaag tcaatgaagc ggacgacggt cacgtcgcgg tcgaagcggt
    17701 ccggcgaatg ccttttgatc tgatcatcat ggacatttgt atgttgaatg tttccggaat
    17761 agaagccttg ggacagatta aagccatcaa tccggaaacc ccggtgatta ttatgacggc
    17821 gtacgcttca ttggaaacgg cggtcagtgc cctgaaaaag ggagcttatg attatctgac
    17881 caaaccgctt aacttcgacg aactgagaat tgccatcgga cgggcgacgg aacataatcg
    17941 gctgaaaagg gaaaacagat atttaagaaa caggctggat gaaaaattta accggcataa
    18001 tctcatcgga cagagttacc cgatggtcaa gctcttggaa acagtggccc aggtggcgcc
    18061 cacggaggcc aatgtgctga tttccggaga gtcgggcacc ggcaaggaat tgattgccaa
    18121 cgccattcat ttcaacagca tgcgggcgga aacggttttt atcaagataa actgttcggc
    18181 gctgacggaa acgcttctgg agtcggagct gttcgggcac gagaagggag cctttaccgg
    18241 tgcggaacgc aggagggaag ggaaattcct tcaggctcac ggcggcagct tatttctgga
    18301 tgaagtcagc gagatgtcca aggcgatgca ggtcaaactt ctgcgtgtct tgcaggaacg
    18361 cgagttgaca cgcgtgggcg gagcggaagt gattaaagtc gatgtgcggg tgattgccgc
    18421 ttccaataag aacttgaaag aagaagttca gaacggaaat ttccgcgagg atttgtttta
    18481 tcgcttaaac gttgtttcct tgaatgtgcc gccgctgcga gaacggaaag aagacattcc
    18541 gatactggcg cagcattttc tcgagttgtt tgccgggcgc aataaaaaaa ccattaaagc
    18601 attcactccg cgggcgatgg aaaaactttt aatctatcac tggccgggta acatacggga
    18661 actgatgaac gctgtggaac ggtcggttgt tctgtcacga acggattatc tggacgccga
    18721 cgatatgacg ctggtcatgg agaaaagcca tgaggtttcc ggtattgcgg agcaggggga
    18781 agccataaga aatttatctc tggaaagcat ggagaaaaaa aatattctgg cggtgctggc
    18841 tgattgcaga ggcaataaaa gcgaaacggc gcgccggctg ggcattacca gaaaaacgct
    18901 gcgcgcgaaa ttaatcaaat atgagatgaa ccggtaatgc ctcccgttca gcgggtcacg
    18961 ctcttgagaa ttgatgagac gaaaataaac gctaagaacc gtttgttgtc tttgcgggca
    19021 gataaatgga aaaggttgtt ccggaattga cgatcgattc aacggtaatt aatccgttat
    19081 ggtatttaat gatggaatcg ctgattgcca gccccaggcc gattcccccg tgttcttttg
    19141 tcttttttgt cgtaaagtag ggatcgaaga tccggggaag attttcccgg ggaatgccga
    19201 tgccgctgtc cttgataata attcggatgt aattcccctg tcccagcgac cggatgttgg
    19261 ttccggtggt ggtattttcc gtggtcatct gcacggaacc gccatccggc atcgcttctc
    19321 tggcgttgat caagacgttt tcaataacct gtttgatctg tgtggcgtca atttcgcagg
    19381 gccagagatc ttccgcgatg gtaatattgc aggtgatggc cgagttttta aaaacacttt
    19441 ccgccgcctc atgcaacaca ggttctattt tttccagctt gcgcaccggg tatcctccct
    19501 gagcgaaggt gataagacgg tgggccagtt ccttggcctg aaggccggca cgttcggcta
    19561 cggcaagacc gttctccagc attttatctt catcactggc ggatattttg gccaggaaaa
    19621 cattccgtaa aatcgcggtc agcagactgt caaagtcttt ggcaaccccg tccgcaaagg
    19681 ttcccaggga ttccagtttc gtccgccgga gcagttcgtc ctgcgctaat ttctgttctg
    19741 tgatgtcctc cagtgtttcc atgacgccga acagatttcc tctggaatct ttgaggcctg
    19801 ccgatgttat gcgataccat ttcccctttt cgcccatgtc aggataaaac tgtgtcacct
    19861 cgtaggcgtc ttccagcagc ttggatctgc tccagcggtc tggatacagt tcggcaatct
    19921 gggcggtttt cccgtccaga atgagatccg ccagacaggg acggggcgat gtgtaaaacg
    19981 cgcgccagtg ctggtctgtg cccagaacgt cgctgggctt gattttgctt gccgcctgca
    20041 gcgctctgtt ccagtaaacg actttgtgat ttttggatat gacgaacgtg gggatagggg
    20101 agccgtggat aatgctgatc agtttctgct cgtcccggtt catacgccac atggtataaa
    20161 taaaactgat gatgacggcg atggaaagaa caaaaacgaa aataaaaata aaaaaattgt
    20221 tcataaacag gatccgctat gtaaaaacca attcatcttc gatttgatcc ggaatgcctt
    20281 caatatttaa tggattttga taacaaggtt aaattaaaaa tgcaagaact gaaaataatt
    20341 tgtttcaacc gggttttttg ccgttaaaaa catcaagcag cattcttaaa agatgctcaa
    20401 cagtggaaaa cccgattagc gatatcagat gcttgaacaa agataaagat gcaacttttt
    20461 cgctgttgat gaagggaaat cgtgtgcgaa agaattttta cagatcttcg ggaaaatttc
    20521 cggccgggga aagatgatct tatccgttgt cttcaaaacg gtaaagatcg ccgaaagaca
    20581 caccaaacgg cttcctgatc aaaacggatg gcggaaagat aattaaccgg ctcagatgtt
    20641 atcggacagc cagtcgccga tttcgctggt ggacatgccc atcttgccgg catcgaggct
    20701 tttgagtttc ccgctttcca gaagtttaac gaccgcggct tccactcttt tggctgcctt
    20761 ttcttcaccc agcgtttcca gcagcatacc gccggcacag atggccgcca gaggattgat
    20821 gatattcttc ccggtatatt tcggcgcaga accgccgatg ggttcaaaca tggagacgcc
    20881 ttcgggatta atgtttccgc ctgctgcgat gcccatgccg ccctgaacca tggcgcccag
    20941 atcggtaatg atgtctccga acatgttatc ggtcactatg acgtcaaacc attcaggatt
    21001 ttttaccatc cacatgcagg tcgcgtccac attgacgtaa tcggttttta tttggggata
    21061 gtcttttgcc agctcattga aggttctttc ccagagatca aaggcatacg tcagaacgtt
    21121 cgtcttgccg cacagggtta atttgttcgc cttgtttctt ttggcggtat actgaaaggc
    21181 gtagcgcaaa cagcgttcaa cgcctttccg ggtattgatc gattcctgga tggccacttc
    21241 atcaacggtg ccttttttaa gaaatccccc gccgcctgta tagaggcctt ccgtgttttc
    21301 ccggacgaca acaaaatcga tatctttcgg tcctttgtcc ttgagcgggc agggcacgtt
    21361 ggggtaaagt ttgacaggcc ggagattaat gtactggtcg agctcgaaac gcattcgcag
    21421 caaaatgcct ttttccagga ttcccggttt gacatccgga tgtccgatcg cacccagata
    21481 aatggcatca aatttcctga attcttcgag aaccgaatca ggcaggatct cccccgtttt
    21541 tttataacgt tcgccgccca gatcataatg aacgagttcg tatcgtatgg attcggatgc
    21601 cgccacggct ttgagcactt tcaggccttc ggcaacaact tccggaccgg ttccgtcgcc
    21661 gggaaccacc gcaattttat aagattttga tgacgtcaca gaaacctccc ggatcatgtt
    21721 cgatcatgaa aaaataaaga ggccgcactc ataacgcaag gaaaggtcaa agtcaatatt
    21781 taagccgttg cgttctccaa aatagttttc ccggccctta atttttgaaa acagaagggg
    21841 agatgataat aataccttta ttctgataat ctacaaagat atcaaatcat tatggctgtg
    21901 aagggtagaa attcttttct gattatacct taaatatttg caagtaaact caaagaatgt
    21961 taatcaataa agccagcaat tcatactggc aaaacgtgcc cgcacagtca ggctttttaa
    22021 ctaatgtgga ggtgattatg ggagaaggaa ttggcgatta cacaaaaaac atagttgatt
    22081 taagaagaca tatgtggatg gccggtgtgg aggttgaact tcgtcgtctc atctggcaga
    22141 ttgccgtcgt cggcaaatat atctcagcaa agattcatga gtccaacaga aagcttgccg
    22201 gactgaagaa tgtctacggc gaggaacaga tggcgctgga tcgcgcttcc gacgagatac
    22261 tgaaaaacca gctggaattt tcgggttttg tccgtgaata cgcatctgaa gagctggaga
    22321 atatcgtaaa aataggcacc ggtcaggaaa aatacatcgt aacggctgat ccgctcgacg
    22381 gctcatccct ggtggatacc aatctggcga taggaaccat tatcggcatt cacaaggaat
    22441 cggttctgga ttgcggacga aataccatgg tggctgccct gtatattact tacggtcctc
    22501 tgattacgat gatttattcg gcaggcaagg gcgcccatga attcgtcctt aatcgcgagg
    22561 gagagtatgt tctgtcgcag gaaaatatca aattgaaaga aaaggggtcg atattttcac
    22621 cgggcggcct gcgccgggac tggaatccgg cgcatcttca gtttattgac aaactggaaa
    22681 acgacggcta caaactccgc tacagcggcg gtttcgttcc tgacatcaat caggttctga
    22741 ttaaaaaggg aggattattc acgtatccgg ctttaaaaaa atcgccgaaa ggcaagctgc
    22801 ggctgctgtt tgaattgcaa ccgatggcct tcatcatgga acaggccgga ggcctcgcca
    22861 tcgacggcga tcaggatatt ctttctgtga aggtggagac gatcaatcag ctctcgccga
    22921 tctatatcgg aagcacttac gaagtccaac tggcaaaaga aatgctgtcg gcatcgtgaa
    22981 aaataatccc ggtgcggaga tggtcggaac gggcgggtga tttccccgcc ggcgactgaa
    23041 taaaatataa ttgcaaggag tgattatgat ttacaaagat aaaaatgaat tgcagaagaa
    23101 atacgaaaaa attattgcca tcgagggcaa cgatgtgagc attttgaatg agacggcact
    23161 cagaggagac ttgattgatc atttgattta taccgccgtg ttcagcgccg atcaggcagc
    23221 acagcaagcc gcatgctggc tgatccgccg cgcaggcgct gccctgggaa ttgtgtccgc
    23281 ctccattcaa cccctttatg aagcgatggg ccgggaagac gtcaggggat ttaccgttcc
    23341 ggcaatcaat ctccgcggcc tgacctatga atcggcccag gcggttttcc gcgcgatgat
    23401 gaaagggaaa gtcggtcctg tcattttcga aatcgcccgg tcggaaattg attatacctc
    23461 gcaacggccg gtggaataca cagcggccgt cattgctgcc gcaatcaaaa ccggatatcg
    23521 cgggcccgtc tttttgcagg gggaccattt tcaaatcaac gccaagaaat ttgcggccga
    23581 cgcagcgaaa gaaacccaga cggtaaaaaa tttaatctgg gaagcgattg aagcgggatt
    23641 ttacaatatc gatatcgata cgtcgacgct ggtggatctt tccaaaccca ccgtcacaga
    23701 acagcaacag acgaatttca gtctcgcagc cgaactgacc accatgatcc gcgatctgga
    23761 accggaggga atcacaatat cagtcggcgg cgaaatcggt gaagtgggcg ggaaaaacag
    23821 cacggtggaa gagctggagg cctatatgga cggttatctg gcggagctta aaaaacaggg
    23881 tgaatcgctc aaagggatca gtaaaatcag cgtccagacg ggaacgacgc acggcggcgt
    23941 tccgctggca gacggcagcg tcgccaaagt caaaatcgat tttgcggttc tggagaaatt
    24001 atcggcggtg gcccggtcca aatacggtct ggccggtgcg gttcagcacg gagcgtcgac
    24061 attacccgac gaggcgtttg accggttccc cgcggtgggg acggctgaaa ttcatctggc
    24121 caccggcttt caaaatatta tttacgacag ttcggctttc ccggctgact tgaaggccag
    24181 aatatacgat catctgaaaa cggcattcca gtctgaatgg aaggataaag atacggaaga
    24241 acaatttatt tacaaaaccc gcaaaaaggg tttcggtccc tttaagcagg atatgtggca
    24301 tttgccggcg gacacccgcg ataaaatcag cgccggactg gaaaaacagt ttgcctttct
    24361 atttgacaaa ctgaaaatga acaatacgca ggatgtggtt gaaaaatatg ttcaacccgt
    24421 ggacgtcgcg ccggatgttc ctgtcagcct gaaatgaaag cggcgatttt agagcaaaca
    24481 accagcttaa agaaaaataa gaagcctctg aaaataaccg aggttgccga acctgcggtg
    24541 ggcgacggtg acgacgtgct ggttaaagtg tcggtatgcg gcgtttgtca cacggaactc
    24601 gacgaaattg aaggcagaat ctttctgcct tcattccctg ttattttggg acatcaggtg
    24661 gttggcgttg ttgccggaca gggcaggcgc gcgcgcaaat ttgcaatcgg cgacagagtg
    24721 ggcattggct ggattaatcg atcctgcggt caatgtcctt tctgtctttc cggaaatgaa
    24781 aatctatgcg ccggttttaa ggccacgggc agggatgtct ccggcggcta tgcccagtat
    24841 ctgaccgtat ccgaaaaatt cgcgtttccc attcccgaaa ttttttccga tagtcaggcc
    24901 gcgccccttc tgtgtgccgg cgccatcggt tttcgttccc tgcgtctggc aaatctgaca
    24961 aacggacaac gtctcggtct gaccggattc ggcgcgtcgg cccatctggt tttgaagatg
    25021 gcccggtatc tttacccgca aacaccattg ttcgtgtttg cccgcaatcc ggcggaacgt
    25081 caatttgccc ttgacctggg tgccgtctgg gccggagata catccgacga atctccggaa
    25141 aagcttcatg ccgtcattga tacgacgccg gtctggaaac cggtggtcga agcgttaaaa
    25201 aatctggaac cggccggccg gctggtgatt aacgcaatcc gcaaagagga cacagacaaa
    25261 gattatcttc tgcaactgaa ttacggacgt catctctgga tggaaaaaga aatcaaaagc
    25321 gtggccaacg tggccctttg tgatattgag aactttctta aactggcctc cgacatgcgc
    25381 attgttccgg acgtgcaggc ctatccgctc aacgacgtca atcaagcgct cggcgaattg
    25441 aaagaaggta aaatccgcgg agccaaggtc ttaataatca gttgattttt caacggcaat
    25501 ctgcgtatta ttaatatatt aatttgtaaa tttttattaa cgctaccata accggaaacc
    25561 gcaacggatt taaagtttgt ggctcttttt tatgccggat aattggcagg aaatgggatc
    25621 gaggtttgcg gagaactcat tatgaaaata gccagtgtcg gtgaaatgcg ttccatggat
    25681 cgctttgcta ttgagcatct gggcattccc gaggacgtac tgatggaaaa cgcgggacgg
    25741 gcagccgtca gcgttctttt gtcgcgcaca ggcattcgcg gcaagaaatt cgccattttc
    25801 tgcggatccg gtaataacgg aggggacggt cttgtcgttg cacgtctgct ccattcacgc
    25861 ggcggccttg tcagggtatt tttactcaat gacagcgaaa aattcagagg tgcggccgcg
    25921 acgaatttcg aaatcgtcaa acggttgccg ctggaaacag cgcagctgac aaatgcccga
    25981 ccggctggca ggcacgtggc ccattgcgac gttgtgattg atgccatcct ggggacggga
    26041 ttggatcgtc cggtttccgg attggttgcg gacaccattt cactgattaa taaaggcgga
    26101 aaaaaagttt tatcgctcga cattccctcc ggggtcaacg gcaataccgg agaaatcatg
    26161 ggagccgctg ttacagccga ctataccgtc acgttcggcc ttcccaaaat cggcaacctg
    26221 ctttatcccg gttgtgacgt caacggcgaa ttatgggtca gtcacatttc ttttccgccg
    26281 tcgctgtatg accgggatga catcccggtg aggacgaatg acgctatggc tttgccgccg
    26341 aggcctgccg atgcctataa gggcgccatg ggcgatgtcc tgttcatcgc aggagcggtc
    26401 aattattacg gagctccgta tctgtcggcc atgtcctttt tgaaggccgg cggcggttat
    26461 gcaagactgg cagcgccgcg tgcggtgatt ccggtaattg ccaggcgcgg ccgggaaatt
    26521 gtttatctgc cgcaggaaga aaccgaggca gggagcatgg ctttgaaaaa caagaaggcg
    26581 ttgctggagc attcggcaaa ggcggatatg gtggtcatcg gtccgggact ttctctgcag
    26641 gacgaaacgg tcaaccttgt cagggaaatc gctgcgacgg ttcgtaagcc gatgctgatt
    26701 gacggcgatg gtcttaccgc tgtggcaggc tgtccggaca gcattcgccg gcggaaagcg
    26761 gcaacgattc tgacaccgca tatgggagaa atggcgaggc tgacgggcaa atcattgaag
    26821 gaaatccatg ccgataaaat aaaagtattg cgggagacgg tgcaaacatt gaacgcctgt
    26881 atcgtgctca aaggaccgca ttcgcttatc ggcacccgtg acgggaatgt ttacgttaat
    26941 ttaagcggta atgcgggaat ggcgacggcc ggttccggtg atgtgctgac cggctgcatt
    27001 gcggcgatgt tcggcatggg gttgtccttc gatgaagcaa cccgcaaggg ggtattcctt
    27061 cacggggtcg ccggagatct ggcggcgtta aaaaaaggaa cggacggcat gacggcgcag
    27121 gatattatga atgcgctgcc tcaggcgctg aaagaagaca ggtccggaag gcttcaggat
    27181 caatatgccc ttcccgacgg cgtttaataa atgttgtaaa atcgaacagg gcgttttacg
    27241 ataggctatg aatcttccaa tgcaaataaa tcggctgacc ctcaaatttt ccggggaatt
    27301 gtcttatctc gaagcgccgt ttctgaaaga gtattatacg tcgtctctgc cgcacatccg
    27361 catgatgatg attctgggag cgatgcttta tgcggcgttc ggtgttctgg atgttctcct
    27421 gatgccgcag caagtcgttg cgctctggat cattcgtttc attgtcatcg gtccggtgct
    27481 ggtgggcgtg ctggcgatat cttttttgcc catatttgaa aaatacatac agcccatact
    27541 tgcactggtt tattttctgg ccggtgccgg catcatcggc atgatcgttg ttgcaccgcc
    27601 gccggtcagc tattcatact acgccggcct tatgctcgtt ttcatgtggg gatacaccct
    27661 catccggctc ttttttacct gggcgtctgt tgccggatgg ctgcaggtta tcttttatga
    27721 aaccgttacc gtcgggctca acccgacgcc gctggatgtt ttcctgggca ataatttctt
    27781 cttcatcagc gccaatctgg tcggaatgat ggcctgctac gcgattgaat tttatgcccg
    27841 gcgcgacttt ctcatgaacc agcaggtgga agaggagagg gaaaaagtct ccaggatcaa
    27901 tgaggaactg gaagaccggg tcaaacagcg tacggaagat tataaaaacc ttaatcaggc
    27961 gcttttgcag gaggttgccg gtcatgagca ggcgaaagag aaactgcagg aagctctgga
    28021 cagtctcaag aaatccatgg gagtaaccat tcaggtcatg gtgtccgccg tggaggcacg
    28081 ggatccgtat acagcaggcc atcagatcag atcctccaat ctggcctgtg cgattgcagc
    28141 ggaaatgggg ttgccccagg aaaaaattga cggtattcaa atggccagtt ccattcatga
    28201 tatcggaaaa ttgtccatac cggcggagat actgacaaaa ccgacaaaat tgaccaatct
    28261 ggaatattcc ctcattaagg agcactcggc ctgcggatat gaaatgctga aaaatgtgga
    28321 atcgcaatgg ccgctggcgg aaatcatttt tcagcatcat gaaaggatga acggttcggg
    28381 atatcccaga cagcttaaag gggatgaaat actccttgaa gcccgtattc tggctgtttc
    28441 cgatgttgtg gaatccatgg cgtcccatcg tccataccgt ccgtcgctgg gaatcggagc
    28501 cgctctggaa gaaatcgaaa aaaacagggg gattctctat gatagcgctg tcgtggacgc
    28561 ctgtctgaag ttgttcagag aaaagaactt ctaccttgag taaagtttct gccattgaag
    28621 atgcctcatt aaattgtttc gtgagaactg aaaaatgaca aaatcattcg ctgaaaatcg
    28681 tgacacaaaa aagaatttga aaaaatatta tattatgtac gatcccacga ccgtggatct
    28741 gatgggaaaa ataggcgtgg cgggcttcac cgttgaagat acggttgaat acctgacggg
    28801 cagaaagctg gaagcgtgca atgaaacgaa ttataccgaa ttcctgagct tcaataccaa
    28861 cctgctccgg aaagggatgc tcgagcagat ggccatcgag ccctataaag cctactatat
    28921 tgccaatcag atttgccggg cggatcaaga cgcaagagtc atcatcgccg atgatacccg
    28981 gaaaacgatc ggacagatca ttgcggaaga aaaacaaaaa ccggtggcag tcttcatgtc
    29041 tctgatcagt tcaacgtttc ccaccgcttg tgccgtgatt ctgattttga acaaagtcaa
    29101 cataccggtc gtcatcggcg gcatccatgt ttcaaccagc tttaatgatg tggacatgta
    29161 tctgagaaag tatctgccgc atccccaaat cgttacgcag gttatcggcg ccggcgattt
    29221 gccgaccatt aaaaacgtta tgtcggatct tgcgcgttcg aatctgaaaa aagagtatca
    29281 cggaaatatc ccgattgagg atggcgtctg gggatgtgac cgggtcatcg aattacctaa
    29341 aatcaaacca tactttatta ataaatttcc cgtcatcggc ccgctccttt cacgaatcat
    29401 tgaaacgaac gtgacgactc cctttttagg ctgtccgttt tcctgcagtt tctgttccat
    29461 atcttcgttc ccggaagagc gaagaaagtt cacatctcgt tcacccgagg attttacctg
    29521 tgaactgctg gataagcaga agaaaggtgt cacgtttaag aaccggttct atttcatctc
    29581 gcccgataac cttttgttcg gggggaaaaa gctgcacgat gttctggaca aaatgatcga
    29641 aagtccttta cggatcaatt acgcggcgca aatcagcatt gaggtcgccg atagtgacaa
    29701 gctccttcaa aaactgaggc tttccggcgc atcgcatttc tttatcggat tggaatcgct
    29761 ggatatcagg aatctggagt atgtcggcaa gaatatcgta gccaaaatca aaaaagagaa
    29821 aatatccgtc gaagagtatt acgcatcgcg aattaaaaaa attcaggatc acggtatttc
    29881 cgttcatggt gcgttcatgt tcggaatgcc ctacgattat tttcattctt tagaggatca
    29941 ttccggcagg aaaattgttg aattctgcaa gaagaataaa atcggcattc agccgacctg
    30001 cctgagcaat ctgccgggct cgcttgattt tcttaaaggc ctggagacgc atgagctgat
    30061 ctacggcaat cccggttcaa tggattattt ctgttcgtta accattgccg atttgacgga
    30121 gagcaaccga aagataccgg atgccctgtt caacagtccg ctggtgactt tttacatgct
    30181 gtacgacacc atggaaaaaa taggatccta catcagcgct ttgcatattg cttttggtat
    30241 ggcgaagaaa tcctggagtg tccattcgtc aaaaggggcg caaagcttca aagaccgcat
    30301 ggtggatgct tttgccggca ttggatttca actgggggcg agtgcctact ttgagctgta
    30361 caaggggctt gcgtattcca caagttcgct tccgggaaca tttgaaagat tatatcaatg
    30421 ggaaaaaaat ccaaccgtga ggaagatgtt caaaaaatac gtggagaatt ttgtctagag
    30481 cgggaattgc attgtccaaa atcacacgca gcgaaaaaga aatagaagag gtacgaaacg
    30541 ccattctcga agaagccctg acgattctga gagaagaagg atttgagaag ctttccatgt
    30601 accaactggg cagacgaacc aatatgacgg cggccaacct ctacaattat ttcagaaaca
    30661 aagatcaact gatcatcgca atccataaaa aaacatttga gttgttatat gccatgcttt
    30721 cgaaggctgt ccggaaagaa gcggagccga tggccaaact gaaaaaaata atcaaaacat
    30781 ttttgacgtt tgctaaaaaa tatccgaatt attacgacat catgtttaat cgccgggtgc
    30841 ctcaggtatc cgactattat ggaacatccg aagaatctct tgcccgcgag gaatatgaaa
    30901 gctcggcgag aaatcttaat ctggtcatga gtatcggcaa gaacgttctg tcctcatctt
    30961 ctgagccgga tgtccgggac gtggagtttt attttatcag gacctttagc gaactgcatg
    31021 gcattgtctc tctttacaac agcggtattt taaaggtgat cgttaaaaat cccgaacgtg
    31081 caatgcatcg tatcgtcgat gatttactga ctgcctacat caggaggaaa taccggtgat
    31141 ctcatagaaa tatcagacgc aagatttgac ttcggctcaa cggcctttcc tgccaatccg
    31201 gtccgctgtt gcggtggcgg cggatgattc tttttcgata cgttatcgct atcatcggtt
    31261 tctgaatcct gacggaaaac gattgacttt taaatttaat agcgttaaat taaagacaaa
    31321 aaactgacgc ttctgcaagg gatgtcaggc aagagaaaaa gccatgcaaa acacgctgga
    31381 aaacagttca gatcaaaatc ggcgatcagg ctattggggt ctggcggtaa gcgcatcaaa
    31441 ggtcggcatc ggtaaagtgc tgcgcaagca tcagttgctt ctggaagggt ttctgcaatt
    31501 tatcgccggc cgattcaata cttccgaagc gctgctcgag gagttgaagg gcacgcaggg
    31561 ctggatcaac ctttgtctgg ggatgagatc ggaggacagc gccatcgaag cggcgatcgt
    31621 gtttcaggat ggcgtggtcc gtgtgctgaa gacaattcct gaaaatgtgg aaacggtcat
    31681 tatctttaaa acctctgatc atcttctgga tatgaccgat gcaacccccg atgaaatgta
    31741 taaaatgatg ctgatcggca ccattcgaac acaaggcaac atcatgctcg cctccctgtt
    31801 caattatctg atggcgcttg tttttgacaa gggacagcag aaagccgtgg accgacagat
    31861 tgaagaacat agaaaggcaa acaaatccgt cggccgggat gtggccgaca ccgattgctg
    31921 caggcaggaa cgtcagagaa gaaaaatcag ccgcgtagcg ggcggaaaag ttgatcccgg
    31981 cgtcaaatat ctggaggacc cgcatctggc cggcttaggg ctggaggatt ttccgaggct
    32041 ggaacaattt cgcgccgagt atttcggtaa aaaaaaggaa gcggtggtct gtcacgaata
    32101 cggaaaactg atcacagatt tccatctggc caacggatac gaggtggata aggacggcaa
    32161 accctgggat cccaacctgc gtaaagcgga aagcctgaag tatatcctgg agaacagaac
    32221 gccggtgatc aggacaaacg atctgctggc cggaacgtac accacatctc ccgtttccac
    32281 ctgcgtcgga catcctttct ccatcggctg ttacagctgg ggtgagctgc ggtccttttc
    32341 aaaaagggag ctcatgccgt atgagatcag cgaggaatcc attcatattc tgcacaggca
    32401 cgtatttccc tattgggcaa aaagaaatat tcacgaattg tggagaagcc ggacaaacgg
    32461 cggtctgccc gttcagattc atgacaggtt tttttccgtt tattattgga aaacgatatc
    32521 catgtcggaa gtgcccccgg gacacgaagc gcttatcaag ctggggaccg gagggttgat
    32581 cagaaagata gaagaagaac ttgccagaga tgctcatgcc gacgatgaaa agaaaaatac
    32641 gttaaaggcg atgatcatca gtctggaggg tgtcaacgcc tacgcccgca atcttgcccg
    32701 tcaggcgctt gaagagtcca aaacggcaac gaacccgcag cgtaaagccg aattggaaac
    32761 catgcatcgg atgctgctca aaattccgga aagtccatct gaaacgcttc atgaggccgt
    32821 tcagaatatt attctgatgc acctttgtct ggggatggaa agcaccgacg acggtcccat
    32881 gtacggaagg ctggatcaga ttcttcagcc gtattttgaa tcggatatgc ataaactcac
    32941 caccccgcaa aaacgggaag catacatcaa acaggtgatc gatattctcg gatgtcttta
    33001 ctttatagaa tccagtcatc agattcttgc tccggatatc ggcaactggc agaacggcgg
    33061 ctcgtcgccc aacggcacga tcacgctggg aggcgtgacg cctcaggggg aagatgcggt
    33121 caacgacatg acatatatcc ttctcaaggt gacggaattg ctttcgttga ataatcccaa
    33181 tgttcatgcc cggtataaac cggataaaaa cagttttgcc tatctgaaaa gggtttgcga
    33241 tgtgaattac atcacaggcg cgacgccctg catccacggc gatgacgccg tcatggaatc
    33301 cttgacggcg cgcggctggg ccgttgagga tgtgagggat tgggttgtca acggctgcgt
    33361 ggaaccgggc attcccggca agcactgcag cgccacgtcc agcatagaat tcaatctggt
    33421 cgccgtactg gaaatggcgc tgaataacgg aaaacatccc ctgatgaact ggaaacttgg
    33481 tccggacaca ggcatcatcg ggcagggcga tttcgagacc tttgacgatt tctggaaagc
    33541 gttcaaagaa cagtgtgaat ttctgtgcga gcaatccatt atcggcaata accaattggg
    33601 cgagatctat caacagcatc agcccgcgcc gctgatttcc agcatgaccg aagggtgtat
    33661 tgaaagcggc agaggggtaa cccggggcgg agccagatat aattcgtccg gcgtatccgt
    33721 tatcggaatg gcggatgttg tcgattctct gatggcgatc aaaacactgg tctatgatga
    33781 gaaaaaaatc acgtttgccg aacttaaaga ggcgattgac agcaatttcg cccatcaccc
    33841 ccggttgcat gtcatgatta aaaccagagt gccgcgtttc ggttcgggca atgaagaatc
    33901 cgtaacaatg gccaaccggg tgacgtcatt ggtgcatgat tattttgcct ctcaccaaaa
    33961 ttatcgagga ggaccccacg cgaccggttg gtggtccatg gccaatcata cggtttacgg
    34021 gcgcgtcacg ggagcctccc cgtcgggccg gttggccggc gaacctttta cccccggact
    34081 cacaccgcat ccgacggcat cgccgaatct gctggataat ctccgggacg tggcgaaatt
    34141 gaaacccgaa acgctggata ataacatcgc cttcaatgtc aaaattgttc ccgcttcctc
    34201 ggacagtcat gaaaggatta ttgaaatcat ggccaattac gcggatacgt atttcaggat
    34261 gggaggcatg cagacgcagt tcaatgtgat ttccacggag acgctgaaag atgcgatggc
    34321 ccatccggaa tattacagcg atttgatggt cagaatttcc gggttcgtgg cctatttcac
    34381 aaggttgcag cgtgatttgc agctggaagt cattcggcgc gctgaatata aaatatagag
    34441 tttatacgtt tcgggagaga atgtaatcgg caacggtaat caggtgtttt ttttcggcgc
    34501 aatcttcaaa aacgtcgagt tgccgtttgg cctctttgat gtatcccgcc gccgtatacc
    34561 gcgacaattc cagcccttcg tatttttcaa tgaatgtccg gacctgcaga gcggctttcc
    34621 tggagctttt ttttcgggag aggatctctt tcatggtgtt tttttcttcg tccgtgcttt
    34681 gtttgagggc aaagatgagc ggcagggtga ttttgccttc ctccagatcc ttgccgatcg
    34741 atttgccgaa tgtctggcct ttggccgcgt aatccagaat atcgtccgta atttgaaaag
    34801 ccatgcccgc gtttttcccg aaccgggcca tggcgtcgat cttttccggg gatgccgagc
    34861 ccaggatcgc tcccgcggcg caggatgcgg aaatcagaac ggccgtcttc ttttcaataa
    34921 tgttgagata gtcatcccgc gtcatgttga tatccccgca tttggtcagt tggaaaactt
    34981 cgccttccga catgatattt cccatccggg agaccagttc aaaaatattg ttgttgccga
    35041 tttcgctcat cagcataaag gccttgaaac acagaaaatc gcccaccaga atactgacgg
    35101 aatttccgaa tactttattg gcggacgttt tgccgcgtct gatcagggcc tgatcaatga
    35161 catcgtcatg caggagtgtt gccgtatgaa tgtattccat gacggtcccc atggcataac
    35221 ggtgttcccc gtgatagccg caaagccggg acgcgatcag cagcaaaagg ggacggaatc
    35281 gcttcccgcc gctgtcgatc agatgatgag ccacttcggg aattaatttt atttcagaga
    35341 ttgcattgtc actgatgcat ttttcgacct gttgaagatc ctctttgtag atggaaaaaa
    35401 tttcttgaat ttgcatcgta aaatccgcct tgttgtggtt accagggatc gacataatta
    35461 tgaacggggc tctgtttttt tcccgttgct gcggatttta tgccccgcat gatccacttc
    35521 cgggtctcgg acggatcgat cacggcatcg atttccaggt aggatgcgat gttgacggcc
    35581 ttgccccgct tgtaaagttg ggccaccagg tcccggtata gctgctcacg ctgatccgga
    35641 tcttcgatgg ccgccagctc ttttttaaac cccgccttga tggccccctc aatgcccatg
    35701 gcaccgaatt ctcccgtggg ccaggaagcc gtgaagaagg attcatggaa acctcctttg
    35761 gccatcgcca tggcgcccag gccgtaaccg cggcgcagga caaccgtaaa atatggaacg
    35821 gtcaggtgcg agccgacaac aaacatccgg cagacatggc gcacctgcgc gcgtttttcg
    35881 atttccggac ccaccataaa tccgggagtg tcacacagag acagaatcgg caggccgtgt
    35941 gcctgacaaa gctgcatcag ccgcaccgtt ttgtccgcgc cctcggcttc aatggcgccg
    36001 gccatatgca ggcagttgtt ggcgatcaat ccgaaaggcc gcccttcgat gcgaatcaat
    36061 cccgtaatca tccccgcgcc gaatttaggg cgcagttcca gatatgaatc cgtatcggcg
    36121 agcgctttaa tgacggttcg catgttgtag gcgcgcctgt tgttttcggg aatgaggtcg
    36181 cgcagctgaa gcggatccgc cgcttcccat gacggcgttg tcccctgaaa ataggagaga
    36241 tatttttttg ctgcggcaac ggcttccacg tcatcggcaa cttcgatatc taccacgccg
    36301 ttttgtgtct ggacgtcaat aggcccgatc tgttccggtt taaaatttcc taggccgccg
    36361 ccttcgatca tcaccgggcc gcccatgccg atattggaat ttttcgctgc aatgatgaca
    36421 tcgcagcagc ccagcagcgc cgcattcccg gcaaaacagg ggccggacac gatcccgaca
    36481 agcggcactt tgccgctgag tccggcgaac atggcaaacg ttgacaaatc caggccggcg
    36541 gtcatcacac cgatggcgtc gacgtctccc ggccttccgc cgccgccttc cgcgaacagg
    36601 accagcggga cagactgttc atgggcaagt ttgagcatcc ggtccatttt tttgtgattg
    36661 aaaaatccct gcgtgccggc cagaaccgtg taatcgtagg ccatgaccat gcagcgggcc
    36721 ttttcttctt tgaagaaagt gccgttgatt gttccgatgc ccgcaatcag tccatcggcg
    36781 ggtgtcctgg ccatgagatc ctcttcggaa cgtctgccgc gctgcgccgc catcgccagc
    36841 gctccgtatt ccataaaact tccggcgtcg cacagatcct caacatttgc ccgggctgtt
    36901 cgttgatttt tctgtctgcg tttttccacc gcctcgggtc tgttttgatc cagaccaaat
    36961 ttctgccgtg caaacatttc ggcaagttcc ggccgcacgg atgtcgtttc ggtctgcttt
    37021 gtattgaagg agacgccggc ttgttcagtt tctttccggt ctggagatgt cgtcatgcgc
    37081 gttctgctcc ctgggttgcc ccgacgtgtt ctatgtcaaa attcatacaa caattatagg
    37141 ggtatttcaa agggtaaatt cagcatttct tgcgcatcat gcgcttatcg gtcggaagag
    37201 aaaccgacgg gctatgaatt catccggaaa tgtgagataa ttttaaagta ttgaaagatg
    37261 acggcaaatc ttctgaaaat atttaatccg gtgaaaagga acaaaacggt tttgatgaaa
    37321 aaatcactct cctgtctgca atgcggaaaa tgttgtttcg tcgattttac ggcttatgcg
    37381 cagcaggaag attatgaccg ctggcgagcc gaaaacaggc aggacattct ggagatgatc
    37441 gaccatcgcc atttgttctg ggcgggcgac agaatgatat cttcggatac gggcaatgcg
    37501 ccgggggaat gtcccttcct ttacaataca ggcaaggtct ggctctgctc catatacgaa
    37561 acaaggccgt taatctgccg tgactatcag cccggatcgt ccgaattatg tccgcagtgg
    37621 aaaaacagaa aaaaaaagga agaataaaat ccaggggcta ggaagacttc cggatgatct
    37681 ttccaaaaag gtcatagtcg tccgctgcgg ttatccggca tttgacgata tgaccgatgt
    37741 ccggcgaagg ccccttgata taggtaaccc cgtcgatctc gggcgcttgg cggcggcatc
    37801 tgccgatata aggataatcc gggcggtcgc ttttcccttc aatgatcacc tcctggacgg
    37861 aaccgatgag cgacgtgttg atggaagcgg aaatttccat ctggtcatgc atgatcgttt
    37921 cacggcgccg ttctttttcc ttttccggca catgatatcg caagccggcg gcttctgtgc
    37981 cttcttctct ggaataggtg aaaacaccga gatgatcgaa ccgggtttgt cggacaaaat
    38041 ccagcatccg gttgaaccgg gccggtgtct cgccggggaa cccggtaatc agggatgttc
    38101 tcagggcgac atcgggaatg atatgccgga tttgagcaat tacctgcctg atttccgcag
    38161 cgttcacttt gcgcttcatg gcgttgagga tggcatcgtc aatatgctga atgggcagat
    38221 cgatgtagga acatatttta tcgttttcag cgatcgtgct gagcaaatcg gcggtaatac
    38281 gggccggatg cgcataaagc agccggatcc attcgatccc cttaatttgc gtcagatcgt
    38341 tcaacagatc ggaaagcctg ggccgccctt tcaaattcca gccgtaaccg gtcgtgtcct
    38401 gcgccgtaat gatgatttcc ctgatgcctc tttgggccag tcgttcggct tcgcggagaa
    38461 tatcatccgg cggacggctg cgcgccgcgc cgcgcaggga agggatgaca cagtaagaac
    38521 agcggttgga gcatccttca gatatcttaa ggtaggcact gaatgccgat gccggtaaaa
    38581 tacgccggtg gttgcagttc attaaataaa ccggccgggt gatcacagcc ttacggcggt
    38641 ttttgcaccg gatgagttct gtgacgtgcc tgacaatgtt gccgacctcg ccggttccga
    38701 tgacccgatc cgcctccggt atctgatcca gaagttccct gccgtagcgt tgtgcgagac
    38761 atcccgccac gatgatccgc aggttcgggc ggtttttttt tgcttcggcg acggtcagga
    38821 tttcatcaat ggcttcttct ttggccggat taataaaagc gcaggtattg atgatgacga
    38881 tatccgcgtc ggtatgtttg tcaaccaggg catggccgga ctggcggaga aatcctccca
    38941 tgacttccga atcaatcaga tttttcggac agcccagact gataatattg atttttgttt
    39001 tctctgcgtt tttcaaccgg gaagctttct aatcagaact tttctgggtt tggcgccgtc
    39061 ggaaggaccg acgatgcctt cggattccat tttttcaata atgcgggcgg cgcggttgta
    39121 ccctattttc atgtaacgct ggaccagtga aatggaggcc tgtcccagtt cgctgaccag
    39181 tgccacggct tcatcgtatt ttgcgtcgaa atcatcgtcg ccttcatgct ggatctgcgc
    39241 ggccgccagg gcatattttt cgatggaatc gtcgtatgcg ggctgtgcct gcattttgat
    39301 aaagtcgacg atgcgggaaa tctccttgtc cgaaacgtat gcgccgtgaa tgcgggagag
    39361 tttggaggtc ccgggaggaa taaaaagcat gtcgccggat cccagcagct tttccgcccc
    39421 ctgctgatca ataatcgtac gcgaatcgat tttggaggac acctgaaacg aaatgcgcgt
    39481 gggaaaattg gctttaatca ggccggtaat gacgtccacg gaaggccgct gcgtggcgat
    39541 gatcagatga attccggcgg cccgcgccat ctgcgccagc cgggtcagcg attcttccac
    39601 atcccttggc gcaaccatca tcaaatcggc cagttcatcg atcacgacga tgatataagg
    39661 cagttttgtg tgcttgattt cctgttcttc ccgggccgtt tcatcgatgg tttgtccgga
    39721 ttccagttcg gggtcgattt gtttcggcat ggccagcacc gttttgcttt tcagcccttt
    39781 gaggagcagc tcattatagg catcgatgct ttttgctttc aaatcattga ggaccttgta
    39841 acgtctttcc atttcttcca ccgtccagcg caacacctgc gacgcctttt tcgggtccgt
    39901 caccaccgga tgcatcaggt gcgaaatacc ttcgtacgag gatagctcca gccgtttggg
    39961 atcgatcatc agaaatttta catcttccgg ttgggccttg aaaaggatgc tgcagatcat
    40021 ggcgttaagc gcgacgcttt tcccggagcc ggtggtcccg gcgatgagca gatgaggcat
    40081 cttggtcagg tcggcaatca cgggcgtccc gacgaaatta acgcccagcg cgatcggcag
    40141 ccggaaagat gattgcgtga aggaatcgtt gtcgatgacc tctttgagga aaacagattc
    40201 tctttttaaa tttggaatct caataccgat gaccgatttg cccggaatcg gggccagaat
    40261 gcggatgctc ggagccatca gcgccagcgc cagatcgtct gcgaggttga cgatcttgtt
    40321 gattttcact cccggagcgg gttccagctc atacatggtg atgaccggtc cgggcgtgac
    40381 ttccaccacc cgtccctcga cgccgaaatc ggacagtttc ttttccagaa tccgggaatt
    40441 ggtgaccagc gcatcgcgtt tgacgcgggc atccttttgc ggggcctcct ccagcagggt
    40501 gaatggcggc aactgaaatt tatcgtcgct ttttaccttc gtaaaatcga aatgtgtctg
    40561 ttcaactttc ttggccggcg cttttttggt cacgtccggc gctttttcga caatagcggg
    40621 ttgcggcggg gacgggggtt cggatttcat gcggttgaag acgaaatcga caaaggccat
    40681 gatgcgctct ttccatctta acaaaaaggc ggcgccggat ttggaaattt tttccgtgaa
    40741 cgagatcatc gaaaaatcga ccaggaaagt cagtgacacg acgaaaatca aaaataaaat
    40801 aatataggtt cccgccacat tgaaatagtt gcgcagcaaa ccgacaatgg cgacgcctgt
    40861 gaggcctccg gtaccgtttt tcaacgattc tccgtaaagg gagatgcttc cgctgatcag
    40921 aagcgccatg atgccggcga aggaaaagat gaaaaacaga aagccgatta aacggccggt
    40981 gccgatgcga aagtcggaag accgaaaata tttaaaactg caaacgaaca gcatcagagg
    41041 gaaaagcaac gaagcggcgc ccagcaggcg gatcagtgaa tctgcggtat acgatccggc
    41101 cttgccggta aaattatgcg tggtgtgatc gcccgcgaca aaatgtgtga aagaggggtc
    41161 ctgcgggtga taggaaacca ggcacaggag caaaaagagg cccagcgcca gacaaaccgc
    41221 gcctttgatt tctcttgttc ttttattgtc cgtctgtttt ttttccatgt tgtggccgaa
    41281 tcaataatag taataccgat attatttcag aaaagagaag attttacaag cagcgtaaac
    41341 gttccgaaga accagccata ataggcaaag tattttaaac gcgcctcccg gataatcagg
    41401 aaaaacagtt tcagggcgag gagtccggcc aatgccgcac aaacgaaccc tgcgaagtag
    41461 agatatgatc ccggggagga aatctgccgt agatagccgg ctttcagaat caccgccccg
    41521 cataccgccg gcagggagag cagaaaagaa aagcgtgccg ccgcgttccg gttcagattg
    41581 cagaaaattc cggacgtgat cgttgtcccc gagcgggaaa tgcccgtaag aaaagggacg
    41641 gcacgggcgc ttccggagag gataccgtcg atgacggtca tatttttgat atcccgtcgc
    41701 gtcttcttca tcttgtctgc taagaacaaa agaatgccgg tgatgattaa gaaaaatgcc
    41761 gctgcccggg ctgattcaaa gatgttgtgc atctggtctt tgaacagata cccgataacg
    41821 gcaaccggta ttgcggcgat aatcaataaa agcaccgcgt cgaaggcccc gcccggctga
    41881 tgaaatcccg ggaaaaagct ctgtacaata accaggtgct caaacctgct gacaggaaac
    41941 agttcggtaa attcctgaac gatgccggga acgataacgg taagcagatt caatgtgccc
    42001 cgctttccag atctttcaga ggagacattt caatatcctg ccaggcattg taatttttta
    42061 ttatttcatt tctgactttt tctatcagtg agtgtctgtt ttcaatcgtg acgtcctgaa
    42121 cgtcgatggg ttttccaatc accattttca actgaccggg ttttatccgg agggaccgct
    42181 tgggcatgat ccggccgctg ccgataatgg aaatcggaat gatcggcact cccgatttga
    42241 cggccagatg aaaggcgccc tgtttgaacg ctttgatttc tccttcgcgg cttctcgtgc
    42301 cttccggaaa ggtcatgacg gacttgccgc tccggatgcg cagtgcggct tcatcaatgc
    42361 tttgcattgc tttttcgcgg ttgctccggt cgatttcaat atatccggcg ctgcgcatgg
    42421 ctgcgccgaa taccggaatg gcgaacaatt ccttttttgc gatccagcgg aattgcaccg
    42481 gaatatgcgc cagtgaaatt aaaatatcaa aatcgctttg atgattggcc ataaaaatct
    42541 gcggttttcc gaacagaatg ttttctttac cgatgacatc gacttttgtg ttgcagatca
    42601 tcagtaatag ctttgcccac aatgttgcca ccttgtgaat tttattgtca gcggcgggaa
    42661 agacggaaaa caccaccgac cagaacgaca tgaacgccgt tatggcaaca cctaatgtga
    42721 ccagtaacgc tgaatggacc ataaacgttt tcctgctcag acggattaat gaataaattt
    42781 ttacttgata ataatagtga agtcaatcat gaaaaaagtt tcaagaatga tgaagtcgtc
    42841 ttcaaacgta tcggaggtag agaataaaaa aatcagggcg ttcagatttt aaaattcttg
    42901 ttcctgaaca gtttcaggca ggctgacacc gcttcaacgt catagagcgt accggcattg
    42961 ttttcgattt cagccagagc gatctctatg cccagcgccg gccggtacgg acggtaggaa
    43021 atgatcgctt ccacgacatc ggcgacggca ataatgcgcg cttcaatcag aatatcctcg
    43081 cctttgagac tccggggata cccggaaccg tccatccgtt catgatgctg gtatacaatg
    43141 tcggcaagcg gccaggggga ttcgacgtct ttgatgattt cgtaactgta ctggcaatgc
    43201 gattttatca aagaaaattc cagttcgctc aacgtggttg gtttgcataa tatttccgac
    43261 gggatggaaa tcttaccgat atcgtggatc gctccggcca tgcggattcc ttcgatggta
    43321 tcttgcggca gtttcatctc cgtcgcgata gcgcgggcca gatcggccac tctccgttga
    43381 tgtccggcgg tatacggatc tcttgcttcc gaagcgatgc ccagcacctg aatggtcgta
    43441 ttaatggatt tgcgcagcgt atgaagggtc tcctgcagtt tcaactcggc ctttcttcgt
    43501 tcggtaatgt cttgataaat gatttgatat cgtttttgcc cgttccagaa tatttccttg
    43561 cgcagtgcaa ggacatgccg cacttcaccg tcttttctga caatgctgat ttcgtattcg
    43621 ggcggaccgg cttcccccat taaccgcttt tcctgccgct gttgaaaatc ggcgtaactc
    43681 tctgcggtgt aacgtttgtg cagaggcgtg ttttttaatt catccacata agtgtaaccg
    43741 taaatatcga gaatggcttg attggcatag agcgtttcgc cttcctccgt gacaatgcgt
    43801 acgcccagag gagaatcatc aagggaatga cggaaatttt cctcgctttt tcgcagggca
    43861 tcttccatct gtttccgttc ggtaatgtcg tgaataattc cgcggaagcc gatcggcttt
    43921 ccggacgaat cctttttcag ggacgccgac tgttcgatgt agcgctttgt cccatccttc
    43981 cggatgattt cccagtcgaa ttcttttgtg gggcgtcccg ttctatagac cttgttaaag
    44041 gcctggaaaa cttttcttgc ggtttcccgg ctggtatatt gacggttgtt cattcccctt
    44101 aattcgtttt cggaataacc cagcagtgca cagaccgggt cgttaaaaaa agtgaaattg
    44161 ccggcaagat ctatttcata atacccttcc tcggtggttt ccagaatcgt ccggtatttt
    44221 tcttcgtttt cgcgttgtgc ctgctgtgcc agattccggg atgtaatatc cgtaataaca
    44281 accaggacat atcgtttttc gcgatgttgg atgatttccg cggaaaaaag accgtcaagg
    44341 atcgtcttgt cccttcgcct gaacttcatt tcgaaatttt caatataacc ctgctgcatc
    44401 agttgtttca gtgcgagctg tcgtttgtcc tgttcgacaa aaatatcaag ttcgtcgctg
    44461 gtttttccta tgacgtcctg ccgggaaaaa ccgaacgtcc gcaaaacggt tttattgatg
    44521 tcgatgtatt ttttttcggg aagtcttaac agaacaatgg aagcagggtt gtgctgaaaa
    44581 aggaaaaggg atttctggag ccagttttca tgcttttctt ccggttgggg tcggtaaagg
    44641 aaggcttctt ttgtgtcttt tctgcttttt actggaggtg ttttttttgc atttgccgtt
    44701 tttatttcct gcattcgtcc atccgtctcg agtcctttgt ggattttcga agatgttgcg
    44761 caacaatgcc ggaatgaaaa tgaataagta tgataaaata taaaataatt tattgcgtct
    44821 gtcaaatatt aaatgcccgg cggatttttt attccaggga catcagggag ttccagcgtt
    44881 cctgctcggt ggcgtgaggc agattgtttt tgcgaagaag atggatgatc gattcttcat
    44941 taaaccgcag gtacgggtta atgccgcgct cttcatccag ggtggaatag gttgtgttcc
    45001 tggagcggca ggcattccgg aaaagatcga tatttttatt ccggggttcc agatgacggg
    45061 cgaacgccag agcattcagg atatagtcgt ggccggcata aatcatcgtt tcaccggaca
    45121 ggtccatcag ccgtttgatg gaaaggaaga aaatattcat gttgccggaa aaacagttgc
    45181 ccacggttcc gttgaaaagc gtgtccccgg taatgagaaa actcccggca tgaaagcaga
    45241 cggaatcgtc tgtatgtccg ggagttcggt agaccaggat gcgttctgcg ttgataacaa
    45301 gaggctgatg gtcctggaaa tcagccgcgc gaatgaattc ggcgcctgtt ttttgcagca
    45361 aatggtcgtt tcccggggta tggtcgtagt gcgaatgcgt gttggtgacg attttcagca
    45421 tgagattgtt ttgcttcaga aaatcaagta tttctttcca tgcgcccccg tcgacggcca
    45481 cggcttgcgt ttttccgtac agaaggtatg agaaattgtc cgttccatac cgtaactgtt
    45541 taatattcaa catggtctta tcccattcga ttcgccgaat aacggctttt cgggtttcgt
    45601 ttttctcccg caggcatatt aatgaagatt ccggggacat gtcaagataa tcataaatag
    45661 ccgacgtcct gttttttaga aaatgtttga caaannnnnn nnnnnnnnnn nnnnnnnnnn
    45721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45781 nnnnnnnnnn nnnntgtcaa gataatcata aatagccgac gtcctgtttt ttagaaaatg
    45841 tttgacaaat tcagatctta ataataatat cacgcaactt tgtaaacgta cgggcgcgtg
    45901 aagcgctttt tttaattttc agcatgatgg tttcgggagt tgccttcaaa cgtgcaaaaa
    45961 ttactgacaa aaaagagagc gttggaaatc atcgccggtt tcccccgcgc gggcgttctg
    46021 gtgattggcg atgtcatggt cgatcatttc atctggggga atgtgtcgcg tatttcgccg
    46081 gaagctccgg tccccgtcgt tgacgttcag aaagacaaca ttttgctcgg cggttgcacg
    46141 aatgtgctca acaacatcca ttccatggga ggccgggcgt acgtttcggg agttgtcggg
    46201 gccgatacga tcggcaaaca gctgctggcc gagttgaatg atcgtaagat tgaaacgaag
    46261 ggcattgtgg ttaaaaaaga ccgtccgacg acattgaaaa cgcgaatcgt ggctcacgga
    46321 cagcagatgg tgcggtttga caaggaagac aggaaaccga tttctccggt aagcatccac
    46381 aagattctgg actatgttcg gtcccgggtc gataaaatag gagcgatcgt catttccgat
    46441 tacaataaag gtgttgtctc gaaggaactc attcagggca taaaaaaaat tgttgatcat
    46501 tcggggattt ttatttgtct ggatccgaaa caaagcaatt tttccgttta taagggcgta
    46561 catgtcatca ctcccaacca ttacgaggtt cagcgcgctg tcggtatgga gctgaacggg
    46621 accaataata ttgagagatt gagcgaaatc attttgaaga aacattcgtt tccggccctt
    46681 ctggtgacac ggggtgaaga aggcgtgagc ttgtttgaga atggccggaa aatcgttcac
    46741 acgcactttc cggcacaggc cagagaagtc tatgatgtga ccggtgcggg ggatacggtg
    46801 atcggtgtcc ttgcgctgtg cctggcggca aaagccagcc tcaaggaagc cacgtgtctg
    46861 gcgaatctgg ctgccggcat tgtggtcgag aaagtcggaa cggccaccgt ttcgcgacag
    46921 gagctgatcg acgtattatg agtaaaccga gtccggccga accggtgaaa ttatttttca
    46981 gcctttttgc gcaggatgcc gtaatcatag acgagacgat aaaagagctt tcccgggctt
    47041 acggggaccc ggattttata agtgcgcttc agccgtttga ttacacggat tactacggcg
    47101 gagaaatggg cgaaaatctg attcgccggt atctgaccat ggaaaaatta atccatccgg
    47161 aatccctgcc ggatatcaaa ctggccacca atgacattga aataaaatcg gcagccggtt
    47221 tgaagagaag gataaacatt gatccgggtt acatatccaa ggctcatctg attttagcga
    47281 ccggcaagag ttacacccac cgtccatatt tgagagacgg aatttatgcc gatttgacgc
    47341 ttgtttatca ggggaaaaaa ttctgtgttc tgccctggac ctatccggat tatgccgatg
    47401 caaaacagct ggagatgctg gaccggatca gaaacagata tttgacgcag ttgaaaatga
    47461 cggatcatta aaaatagtga ataataatcg aacaaagaca ggtttattaa aatgattaaa
    47521 agcatgaccg gctacggacg ggcggaaaaa ttttttcagg acaaaaatat cattgttgaa
    47581 gccaaatccg tgaaccatcg ttttctggaa atcgtgctgc gcacgccgtc ggcacttttc
    47641 ccgatggaga tggactataa aaagaaaatt accgaaagat tcaagcgcgg acgcattgaa
    47701 gtattcatca agttcgaagg cgaaggtgcc gacacagcga aagtgaatct gaatatggaa
    47761 atagcgcgag ggtattttaa tgtattaaac agacttcggg aagaattcga tctggagtcc
    47821 cccgtgacgc taaaaacatt gaccgccttt cgcgatattt ttacgccgcc cgcggaaact
    47881 gaaatcggct ctgattttct ctgcgaggtg gaaaaatcgt ttctcgaggc cttggaaatt
    47941 ctggccgcga tgcggcaggg ggaagggctt gtcctgttgc aggatatgca aatgaggctg
    48001 aaaatgatag acggtgttct taatgatatt gccgtgcgtg cgccgcaggt cgtttcggaa
    48061 tatcagaaac gcctctcgga acgcattaaa gaactgaccg aagggtttgc gcttgatgaa
    48121 aaccgtttgg ctcaggaagt ggccatcatg gcggaaagga ccgacattac ggaagaaatc
    48181 gttcgcctgc gaagtcatct gggccaattt gaagttcttc tgcacagcga tgaatccgag
    48241 ggcagaaaaa tcgattttct gctgcaggaa atgaaccgcg agatcaatac catcggatcc
    48301 aaggtcggcg atgtcgacat cacgcgtcat gtcatcgacg tgaaaagcga actgggaaaa
    48361 ttacgcgaac aggcgcaaaa tatagagtga ggctatgtcc aaaggattat tcatcgtcgt
    48421 ttccgcgccg tcaggcacgg ggaagagcag catctgtcag ggctttatgg aagcgtttcc
    48481 ggaaataaaa ttttcggttt cgtatacgtc gagaccgccg cgtcccggtg aaatagacgg
    48541 aaaggattat aattttattt ctcgtgaggc gtttcggaaa aaaatcaggc agggcgagtt
    48601 cattgagtgg gtggaaaatt acggccacct gtacggatcg tcgcggcgct tcatagaaga
    48661 aggtgtgcgt gacggcaggg atctgctgct ggatatcgaa ccgcggggcg ccaaaaaaat
    48721 aaaacaggaa ttccggagag gcatttatgt ttttattctt ccgccgtccc gtcaggaact
    48781 attgaaaagg ctgaaaaaac gaggtcatga gcgtgaaaac gttattcaaa aaagatataa
    48841 gcagtcggaa agtgaaataa aagagatttc ctggtatgat tacgtcatct ttaacaatga
    48901 tctgaaaaag gccgtcaatg aattgattgc catttatacg gcggaaaagt gcaagaggac
    48961 aagattaaac agagaaataa gagaatttat aaaataaata tatacttgaa ggagatgtaa
    49021 acgctatggc gagaattacc attgaagatt cgttgcgagt ggccaaaacc cggttcggtc
    49081 tggtgtccct ggcctccaaa cgggcgcatc agctgctgaa gagttcgcag cctctggtcg
    49141 tgagtaaaaa cagagaaatc gttcaggcgc tgcgcgaaat tgctgcaggc aaggttgttt
    49201 atgccaatcc cgaatatctg aacggaacca aagaggattt caaaccgatt ccggacagca
    49261 cagagtttat cggcgatgaa gaatatatcg agtaagaatg caggcgagag attaatcttc
    49321 gcgcttgatg ttcagaatta tgaagatgcg ttatcgctgg tggacctgtt gtccggccat
    49381 gtcggaatgt tcaagatcgg caaagaactt ttcacagccg ccgggccgaa aatcatccgg
    49441 gcgatcaagg aaagaaatca aaaagtattt ttagatctga agtttcacga tattcctaac
    49501 accgttgccc gtgctgccga agccgccgtc aaaatgcatg tggacatgtt caacgttcac
    49561 gcgtcgggcg gttcccggat gatccgagag tcggtttccg cctcatggac gtgttccgat
    49621 gcactcggtc gtgccaggcc gatcctgctg gcggtgacgg tgctcaccag tctgaataac
    49681 gacgatttga aagaaatcgg ctttcaaaaa acaacaaacg aactggttgt tcacctggcc
    49741 gggttggcga aggcggccgg agcatccggc gtggtggctt cagcgcagga tattgtcatg
    49801 ttgaggaagg cattcggcga ggattttgtc attgtcacgc cgggcattcg cagtgcggcc
    49861 gaaatccgaa aagacgacga tcagaaaaga acgctcagcg cgtatgacgc tgtggcagcc
    49921 ggcgccgatt atattgtcgt cggcaggcct attcgcacgg cagccgatcc cttgaaagcc
    49981 tgccgccaga ttgtccagga aattgccgaa gggctgtcgg cacggtaatg cctttccgat
    50041 cgcggaagat accatggagt tcatttacgg cataaattcc atcagagatc tcctccggct
    50101 gcagaaaagc ggatgggaaa aaatcatgat tgcctccgga aggaccggcc ccgctgtgca
    50161 ggacattatc gctatggcgg aagctcacaa cattcccgtg gaattttgtg aacggaaaaa
    50221 actggataaa ctggccggaa cacccaacaa tcagggcatg atcgcttttc gtcaaacatt
    50281 tgcttacgcg gatctggaaa ctgtgatgaa aaatcgtcac cccgcgtgtg gtcacgatct
    50341 gattctcatt ctggacagcg tcatggatcc tcaaaatctc ggttcgataa tccgggcggc
    50401 tcattgctgg ggggccaacg gcgtgattat tccggaagac agatcggcag gggtgacggc
    50461 ggccgtgaac aaagcctccg ccgggagcgt tcatcatatc cccgtcgcca aagttgtcaa
    50521 cctggctcaa ttgctggatc acctgaaaga tcgggggttc tggattttcg gtgcggagac
    50581 gcacggcggg aagaaattga cggaaaccga ctttgactgt cccgtcgctc tggtgatggg
    50641 aggcgaagca aaaggcatca ggcctctctt aaagaaaaaa tgcgactttt tgaccacgat
    50701 cccgctgacc ggtaattttg attcgttgaa cgtgtctgtt gccgcaggaa ttattttgta
    50761 tgaaatatcc cgtcagcgaa cccggtcaac taaacaaagc ggcaaatgaa ttgatcgtca
    50821 ccgtcttttt gatgcgtgca tcgtgcgcag ggaataagcg attttggaaa atggcctgcc
    50881 ggttcgacga aagacggtta tttaacttgt tgaatttgta aaaaatctgg tttaaaatgc
    50941 gaaaacttta agttatccga ttgggggaaa ggactaaata tgcctgtatt tatctgggaa
    51001 gcggtaacga aaaaagatga agccaaaaaa ggagaaatgg aagccgtaga tgaaattacc
    51061 gttcgcagtc ttttacgccg ccagggttac aaatccattg aagtcaagag aaaacccaaa
    51121 gatcttcttg aatacctgcc ctttttacag gggaaggtta aggaaaaaaa cgtcgttatc
    51181 ttctgccgta ttttttccac gatgatcaac gccggtcttc cgctgataca atgtctcgat
    51241 cttctcgccc agcaggagca aaacaaggcc ttctccaaaa ttatcaagtc gatcaaagaa
    51301 gacatcgagg gcggaacgag tttaacgaac gcgttgaaaa aatatcccga aatcttcgat
    51361 gaactttttg tcaacctcat tgccgcaggt gaagcgggcg gtattctcga tatcattctg
    51421 caaagacttt ccagctacat ggaaaaggcg atgaaactga aagcgcgtgt caagggtgcg
    51481 atgacgtatc cggccgccgt tctggtgatc tcctgtgcgg tcgtggccct gctgctgatc
    51541 aaggttattc ccgtttttca aaaaatgttt gagggaatgg gcggggaatt gccggcattg
    51601 accgccgcgc tgattgctgc gagtcagttt gcacaaagtt actggtacct tggcgttgcc
    51661 attctggtag taatttacat tattctttcg aggttttata gaacggaaaa gggacgttgg
    51721 acgattgatt cgctgattct caagtcgccg gttttcgggc cgctcttgaa aaaagttgcc
    51781 gtggccaaat ttgcgcgcac gctgtctacg atgatgagct ccggtgtgcc cattctggaa
    51841 ggtttgtcca tcgtcagcaa aacgtccggg aatgtcgtta ttgaagatgc gctcatgaaa
    51901 acacgccagg gcatcagcga aggtcagtcc attgccgaac cgctgaccaa aacgggaatt
    51961 ttccctccca tggttgtgca aatgattgcg gtcggtgaag cgacaggcgc gctggattca
    52021 atgctgtcca aaattgccga tttctatgac gatgaggtcg atgctgcggt ggatgccatg
    52081 acggcgcttc tggaaccggt gatgatggtt tttcttggag gcgttgtcgg cggcatgatt
    52141 gtggcgatgt atctgccgat cttcaaactt gcatccgttg tcggttgatg cgaagctcct
    52201 gccttacagg atgccatcgc gggcatggtg cttgtcgttt tctacagagg gcgtctgacg
    52261 gtttgcagct ggagatgatc ctgtgagctt tgccggcggg attgtttcgc aaacgtttca
    52321 gagaccgcgt cgcctatagc gaccccatgg tgccctggag ggtattcagg tcattcactt
    52381 tcgggataat tcgatcaaca catttttgag tacgcctatc gtgccctttg ggctattgag
    52441 gggatacgct tttgaaatcg ggacttcata aaaatggtcg ggatggcgcg atttgaacgc
    52501 gcgactcctg cgtcccgaac gc
//
LOCUS       DAOSME010000009        52131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_835_length_52131_cov_21.187054, whole genome shotgun sequence.
ACCESSION   DAOSME010000009 DAOSME010000000
VERSION     DAOSME010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 52131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52131
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_835_length_52131_cov_21.187054"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..130
                     /gene="ffs"
                     /locus_tag="PLG98_02355"
     ncRNA           33..130
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLG98_02355"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            282..1979
                     /gene="dnaX"
                     /locus_tag="PLG98_02360"
     CDS             282..1979
                     /gene="dnaX"
                     /locus_tag="PLG98_02360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOE32122.1"
                     /translation="MEYQVLARKFRPQTFEEVAGQAHVVKTLRNAIAQGRVAHAFLFS
                     GPRGVGKTSVARILSKALNCEKGPTPTPCNQCSNCIEITNNSSLDVHEIDGASNRGID
                     EIRELRENVKFAPASSRYKIYIIDEVHMLTDPAFNALLKTLEEPPAHVVFIFATTESH
                     KIPATILSRCQCYDFRRIPLKEIIANLELVASKEGINISPIALSWIAEAGDGSMRDSQ
                     SIFDQVISYAGLDVKDADVEESLGLAARQYLFRLSEAVLNQDAGSCLIILEEAYLAGI
                     DMKYFYQMLLKHFRNLLLVKIAGDKSASFDIATEQIEKLNTQVQTTSRETLQRYLEIL
                     IAEEGNFRRSQEPRMKLETIIVKMAYLEPIMPLGEILSTIETIEKKLQSGLSGNDEKT
                     VPQSIAAQGSSGGMCSAGAANSFSGECKDENHCPPDNAADLKGLRDNLKKFIKKESPI
                     LAAKIESAEILRCENNCLTIGFPANFIFLDEMKNVQKQRLEQIAGKFFQQNMTLVIET
                     LSAENGGANGGRPRSKAGILNDIKQEAMKQPLLRKIIDELPDARVIDVRVQTEKTKS"
     gene            2023..2331
                     /locus_tag="PLG98_02365"
     CDS             2023..2331
                     /locus_tag="PLG98_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOE32123.1"
                     /translation="MQNFNTIMKQAKKMQERMASLQKELETKTVEAQAGGGMVSVVVN
                     GKYEIVSLKIEKEVVNPEDIEMLQDLIAAAVNEGVRKSQEMAAAEMGKITGGLNIPGF
                     "
     gene            2345..2944
                     /gene="recR"
                     /locus_tag="PLG98_02370"
     CDS             2345..2944
                     /gene="recR"
                     /locus_tag="PLG98_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOE32124.1"
                     /translation="MKAYAQPIKKLIAELGKLPGIGEKTATRLANYILRSTEDDVIKL
                     AQSIVEVKKKIKLCRICLNLTEDDTCHICRDASRDQEVICVVEDPDALAAIEESGGFK
                     GIYHVLHGALAPLDGIGPDNLRLGELVQRINRGNIKEIILATNTNVQGESTALLLTEM
                     FKEKNIIITRIAMGMPMGGDLKYIDKMTISKSLEYRRGM"
     gene            3227..4099
                     /locus_tag="PLG98_02375"
     CDS             3227..4099
                     /locus_tag="PLG98_02375"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOE32125.1"
                     /translation="MATEITTKTKLRKINAKKITKAVKKLFIEANTVLGEDVVEALHQ
                     ALSREESSSGRQVLQKIIENADIARRKKMPICQDTGLAVVFVELGQDVHITGMPLHAA
                     IEEGVRQAYADGYLRKSVCDPFTRNNTTDNLPAIIHVDVVAGSHLKIIAMPKGGGSEN
                     CSAAKMLTPAAGIDSIKKFVLDTVENAGANPCPPIIVGVGIGGSLEQACVMAKKALLR
                     PVGEQNRVDERAEQIEKELLESVNNLGIGPGGLGGRVTALAVHIEMMPCHIASLPVAV
                     NIQCHVARHKETTL"
     gene            4395..5237
                     /locus_tag="PLG98_02380"
     CDS             4395..5237
                     /locus_tag="PLG98_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOE32126.1"
                     /translation="MNKHPRHMKISELSAFSKTPITTIRYYILQGLLPVPLKTAKTMA
                     YYTEEHLNRILEIQKLKNENIPLMIIKKKLADNINNACVVTEDSGELLTKTRDEIIRS
                     SVKLFRKKGYDAVKISDIASHAGIGKGTFYQYFSNKEELFFECLENIFLDIGVDVPEI
                     QSETDALKRLRIRGYHFYQNFVHMLDMLNIARRASLINKPEYKEKLEKAFFNFVEPIR
                     QEIELILEQRNSPLKNSTLVAYLLMGAAEYMYYFLLNHKKTPEYIEKEFWNFFFGSVP
                     FQEG"
     gene            5244..6071
                     /locus_tag="PLG98_02385"
     CDS             5244..6071
                     /locus_tag="PLG98_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOE32127.1"
                     /translation="MKKTGVMQISELAKVSGVPVSTIRYYIREGLLPQPLRTGKTRAY
                     YSSDHLKAIEFIKRKQAVGNKSLDVIRKEIEKKFPQQENASDVQIPPGHREKILSSAT
                     ELFSGKGYAETSIADIASHAKISKETFYVHFKNKEELFIACADIIFHNMYNNVWQEIK
                     DEKNIIERSKKRAKAFIVSFPKWITMMNLVRSLSVGNNPVLREKFKHLLSQMINPISN
                     DYESFKRSSNIHKRTDSIITGYFIMGMAEYGAMLIDRGVCTEDEVIDYVTTIVMQGL"
     gene            6312..7493
                     /locus_tag="PLG98_02390"
     CDS             6312..7493
                     /locus_tag="PLG98_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOE32128.1"
                     /translation="MSKGKIAIIGIGEVPTRNMPERTHWDIIYDTCMEAVRDSGLNKN
                     DIEGAISVTPQAQPKIAAELSFGKLPEELGLKGCKDAVSVNAGGASTSNAIRLAEQWI
                     TSGIAKAVLVHHVTVHSTIPIPDLINFFARAGVDLQWEYPYGTTYNIIMGLMANRYIK
                     ETGCKPEEMASVVTALRSWAAKDPNSFFYGKEVPSVEKVLASALLNTPLHKRESNVLG
                     DGGSAMVIVSAEDAKKMKRPAAYKLGDSVRYFSGTCVMRDFYKVTEGFIVTAKEAMAQ
                     AGITKEDVSLWNCYLAYPLAHPLIAELLEVAPQGMGGKYFLEGRMSFGGDIPWSSIGD
                     APGRGHTGSGVSAATYVETARQLMGKAGERQVRNCKYVYQNAGGGSGFNNIATIWGKE
                     I"
     gene            7496..7912
                     /locus_tag="PLG98_02395"
     CDS             7496..7912
                     /locus_tag="PLG98_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOE32129.1"
                     /translation="MDFDMTKPVPETQPWSEKFWEGAKEGKLLIQVCKDCKSKIFYPR
                     KFCPECWSGNLGWIEATGKAKIFTFSTAYDMVEPKFTDELPYTIAYVDLDEGIRMMTR
                     IVDCKPEDISIDMEVEVVFQERGGYFLPYFRPAGKK"
     gene            7925..8752
                     /locus_tag="PLG98_02400"
     CDS             7925..8752
                     /locus_tag="PLG98_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE32130.1"
                     /translation="MPEYTTITYEIKGPVCTITLNRPDKYNAINREMSAELLDAFRKI
                     RDIPDVAVVVLAGNGKAFCTGGDLSVFPSLAEHQESLNWLAHDGYGLGKAIELCEKVV
                     IAKVTGHCLAGGLELALMCDLIYARESAKFGTTEINMGILPGWGGTVRIARAMPIYRA
                     REIIYSGRKDFPARDMYEMGFLTRVFKDDEFEAKFSEVVTNISLKKPIALRMGKEVMG
                     RSLECGSLDAALALERNAIQWLIYAPDIQSVMDTLREKPEVLVAAQKQANVASDKKK"
     gene            8765..9928
                     /locus_tag="PLG98_02405"
     CDS             8765..9928
                     /locus_tag="PLG98_02405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE32131.1"
                     /translation="MDFGFTEEQIMMQQLTQKFCEKELDYKYVKWMDEHVDFPPDELW
                     QKFVDMGTFYGSIPTEFGGQGLGQVDIMVAYEQICKRSMSVALAIGVTAGFGVRFINE
                     LGTPAQREKYLPLIGEGKFKTCMALTEPAGGTDILGAIKATAEDKGDKWVINGQKIFI
                     TGAHVADSLITVCRTEKGAKPSKSLTIIMVPRETPGITIRKIGKISCHHCESVEIFYD
                     NVEVPKENMLGTRGNAFYEIMTVLNPERIGVAMMGVGIISAIYDLCFKYVQERIAFGG
                     PISRFQVLQNYLADMYINLENARNLTYKSAWLCDHDKPFHLEATMAKLVAAEGARHAA
                     TFGSEIFGGYGICNEYPVSMFVRDAYQIQFSPISNEMSRNVLMQFQGLPKSWP"
     gene            9947..11509
                     /locus_tag="PLG98_02410"
     CDS             9947..11509
                     /locus_tag="PLG98_02410"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOE32132.1"
                     /translation="MYTLGDIPRCGAVLWPDNIAVVFEGKRYTYKQFNERINRFANFL
                     IKSGCKKGDGLAVMADNCAKYLEVYFAAAKIGMRVTPINVRLGDDEIMFIVNDSETVF
                     FIVGDGYEEKVKQMKNRFKSIKAWVSLDNPVCGFLDYDKVLDISPALEPDLDQYDIQE
                     DDLAILMYTGGTTGLPKGVMLSHRSALLAGITATIANGFTEDDSTCFVLPIFHVSWWP
                     ILAILIVGGKVCINRKPSLDTIFKLVDDEKCTHMNLVPTIYGWMVDWPSIVNYDLSSL
                     RILTYAGSPFPTEVLKKCIRKFGNKFAQGYGATETAGAGISGLAIKEHQLDGLKQKYL
                     VSAGKPMPNSRVRIVDENDNPLGPGEYGEICAKGKHIMMGYWKNQKLTEAVLKGGWYH
                     TGDMGYMDQDGYIFMTDRKADMIISGGENVYPKEVEDVIYQHPAVRECAVVSAPDAKW
                     GEVVQAVVVLHPGKEATAEEIIEHCKKHLAGYKCPKAVAFWNDIPKTIIGKIVKKDIK
                     KKFWEGKERMIS"
     gene            11537..12574
                     /locus_tag="PLG98_02415"
     CDS             11537..12574
                     /locus_tag="PLG98_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE32133.1"
                     /translation="MSKKNVDNYFKIDPECHLIGDMEHLNHFPGVQMWWRAIEHISRP
                     LITGAPLPLMMKALEGLEEWEAVKSGDPQVILRVMDKHGVDIACLLPESMMDTTGFSS
                     RWVSNGEMARVVETHPDRFMYQPNISPIKQRGVKNAIWEMEYWVKEKGARIFKYYSPE
                     DTYMNDPALWPFYEKAQEFGIVLDMHTGFCWVPPGKSKYCHPEQLDDVARDFPELKIN
                     AFHMGYPYSDVLNMVALGHPNVYICMSLLVPWAITAPRKFAHLLGEAMRFVGPDRITW
                     GTDYAGYGVQVANAVKGLSEFQIPEDMQEAYGYPAITEEDKTKMFGGNLGRLLGIDTT
                     KRRIKQNLKGV"
     gene            12578..13948
                     /locus_tag="PLG98_02420"
     CDS             12578..13948
                     /locus_tag="PLG98_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOE32134.1"
                     /translation="MTEKSEGKKQFAHILKPIKIGPIELKNRIVMAPMNEALSGAHGE
                     VGEQYIAYFGARAKGGTGLITTGAIMATKLAGEFPAGRNPHLYHQGHMLGMNNLTDRV
                     HYFGSKVAAQMTIGFGRQGHSYDHHKLAPAATAGLPYEIAAEKSPNHCADLYAVMEFS
                     RYYMKGQMTREMSIEEIRSEQKEFANSCQLAVISGFDAIEIHGPHGYLEHQFLSPFSN
                     KRTDMYGGEWRNRKRFLMEVAEQVRYACPGVAVGCRISAEEHMQGGLTEEEMIDVAKD
                     LQTIGLDYISLSDGAGYEESGHLITDMDRSKHIPEHGEAFKKALNIPVVVSSQHDPVK
                     IEKNIAEGKYDLQAMGRQLYCDPEYVNKLEAGKAKEIVRCSRCNTCLMRFLAALTPAC
                     PQNPNLGREYALDEYKIGPWQKHESIMPEGTMRASMPSLDRPWWRNELGYIEKCWRPL
                     QGRTSR"
     gene            14274..15098
                     /locus_tag="PLG98_02425"
     CDS             14274..15098
                     /locus_tag="PLG98_02425"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE32135.1"
                     /translation="MAVKGCILVTGAASGIGKATVLTLAKKGFGVYATYATDKSQAEL
                     EKLREVGGIEAFKLDITNDGDVESAVAFIEEKGINLLALVNNAGIFSPGPLMEYPVER
                     LIEQYNVNVFGTHRLTRALFPLLLKSRGRIVNVSSVAGFVATPFSGPYASSKHALEGW
                     SDSLRRELMPWGVKVIVVEPALIDTPLWDKDLEGRIERYRGSIFYEANRKKLEHEAAE
                     AKTRGVQPEIVAEAIHRAVTAPNPKPRYLVTNHPVQHRMVKILPDTLLDRLVIKEF"
     gene            15102..15944
                     /locus_tag="PLG98_02430"
     CDS             15102..15944
                     /locus_tag="PLG98_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE32136.1"
                     /translation="MKMYFLSTGFGHVAKGLFVQGAGMKSVQFPILSVLIERANDLVL
                     FDTGIGTRIEEEMRPPIYWGNLFFHRCVMRTRFDPQHDALVHQLSRLSFQPSDVKNVI
                     ISHLHWDHAGGMRDFPHAHYFVGRKEWDFASRLTNRALFKNAFIKEQFCGAGLDIELI
                     EADPRTPFKNFPASYDLFGDGSMVLVDLPGHSPGLMGMYLTMPSGRKFFFSADTFYFP
                     EGLEQRMPKSKLMQSLVSEGPESEASINRVYDLMKSDPQLEIVGSHDYRLPGRYELAA
                     NFYE"
     gene            15987..16814
                     /locus_tag="PLG98_02435"
     CDS             15987..16814
                     /locus_tag="PLG98_02435"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE32137.1"
                     /translation="MNLKGKVALVTGASRGLGQTIAMTLGEAGADVVLTDILLEGQYH
                     DTEKLGEYSLLAGHFAQQNTVKTQAGAQEIQSMGSRSRAFKMDVVDPEEVASVVQAVE
                     AEFGTIDILVNNAAVMDNMGKFGEQQLERWQRDLNVNLTGAYHCSKAVWPGMVKKSWG
                     RIINISSVAGLMGASLQPSYGATKAGLIGLSKSLAIEAGRYGITVNTVCPGFIATEAV
                     KLYDERMTERIVKRTALKRMGRPEEVAEVVTFLASNAASYITGAVIPVTGGIDLLTL"
     gene            16879..17874
                     /locus_tag="PLG98_02440"
     CDS             16879..17874
                     /locus_tag="PLG98_02440"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOE32138.1"
                     /translation="MNIIKKGKGELFTDPDPDRARTFFQKKSKKMVNKAMTLSKAIET
                     FVHDGEYFALGGFGANRTPIAACHEILRQGRKNMGFAGHTASHDFEILCAGEVFDRVD
                     AGYIVGLEARGLSVCARRYMQTGKVKVTEWTNYSLSARLRAAAMGVPFIPTRNLLGTD
                     TFECSAARIIECPYTGKKLALHPALYPDVGIIHVHESDVYGNVRVRGILKSDNDLARA
                     SKRVIVTCERLITNEEIRRDPTETTFPYFLVDAVCEVPYGGYPGTMAYEYFSDEEHLQ
                     EWLKAEEDSAVFKDFLQRNIYDCKDHHEYINKNGGMEKMLQLRTIEHLLHKEGKR"
     gene            17871..18644
                     /locus_tag="PLG98_02445"
     CDS             17871..18644
                     /locus_tag="PLG98_02445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOE32139.1"
                     /translation="MTTSYNAMELMICVASRELEDGSTLAVGTGAPCAAAMLAQKLNS
                     PNLAIMFEAGSVSPQLPEMPISVGDSRASHKAVMVSTMGAIMETCQRGYVDYTFLGGA
                     QIDIYGNLNSTYIGGTYQKPKVRFPGSGGANDFGSFCWKMMVLTVQDARRFVEKCEFI
                     TTPGWLQGGNSRYEAGLPEGTGPYKVFTNMAVMDFEPESRRMRIISTNPGYSVKDVQD
                     NCGFELLKAPKITETLPPTEIELKILREEVDPHRYVIGR"
     gene            18710..19351
                     /locus_tag="PLG98_02450"
     CDS             18710..19351
                     /locus_tag="PLG98_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE32140.1"
                     /translation="MDRLAQTFSALEEDRVYQLVNECLEKSITPLLIVNELSRGMEKV
                     GQLFKDDEYALAELIYAGEIFKEAMEKIKPLLLAGESRSAGLVIMGTVEGDIHDLGKN
                     IVITMLECSGFKVIDLGVDVPAKKFVEAIKESGASLIGMSLLLTTAMDSLRNAIMAIE
                     EAGIRNNIKIMIGGAPVSEHLRVETGADYLGHDAVEALTIARKVFSPSTVDAR"
     gene            19355..20473
                     /locus_tag="PLG98_02455"
     CDS             19355..20473
                     /locus_tag="PLG98_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOE32141.1"
                     /translation="MKAAERINTVINLECPDRVPLAPLLDHWAATYTGITNAELMSDP
                     DKRFNAVLKTAIDFKWDMSFLADTVNTTLLKLGVPARLKLPGIDLPERSEHQFDEKEV
                     MTEEDFDILESDGLIALFSKLIPRIYPEMTVESAMTDFARASTEITDQAAWLSENGIE
                     PAVGFVIPGPSFEYFCFARSINVALTDLRRRPEKLKIAGKRFCQDMLDLAIASSGQNN
                     ISRVFIGLSRSSPVFLSNKIFEAFVLPDLEVLVNGLIDANRTPVFHCDTNWTKFLPYF
                     QRFPKGKCIIELDSFTDIFKAKEILGGHMAIMGDVPSTLLAEGTRDEVMCYCKNLIEE
                     IGGGGGFILSSGCSVPLNAKVDNVRAMSESIDEWGWYK"
     gene            20528..21529
                     /locus_tag="PLG98_02460"
     CDS             20528..21529
                     /locus_tag="PLG98_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOE32142.1"
                     /translation="MKINMNQWMQNILKSEKRKAFPLMPYLGLELTGKKILEAVKDGQ
                     IQSRCVQAIASRFPSIASVTIMDLSVECEAFGGTIRFVENEVPTTIGAVVNDMNDAEA
                     LKMPEVGAGRTGVYLHAAQLTAQATTDRPVFGCHIGPFSLAARLCGMTEIMVKLRKEP
                     VMVETVLNKCTEFLVQYAKAYKKTGVNGIVIAEPAAGLINAAQCEKFSSSHVSRIVSA
                     VQDENFLIIIHNCGNTYKLVPAMLATGAMGFHFGNAVDMKQIMPQVPAHIPVFGNIAP
                     ILFRNGSSMEVREKTTELLMAMKDYPNFVLSSGCDIPPGTPLGNIDALFDALADYNT"
     gene            21551..22276
                     /locus_tag="PLG98_02465"
     CDS             21551..22276
                     /locus_tag="PLG98_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOE32143.1"
                     /translation="MMREIIKEDPKKIKIDIQEIHAFRKRDKSSLIPETITRHVEKAY
                     DLFCQFAVPVSIVTEVTYSQFQSIYSGDGLNEPRAPLNDIMTDNAVYLLFAMTLGADV
                     SLYISDLFKKGEHLLGHLLDEICSIGIEVFADEITGMCFAGEVSRGTLNHNDIMLLYS
                     PGYCGWHITGQKFLHKYLKSQDIGIRLTENCYMEPIKSISGVMIGCSTLSHYFNNNYP
                     FCIDCRTKTCRQRLVVNGIRNSI"
     gene            22451..23467
                     /locus_tag="PLG98_02470"
     CDS             22451..23467
                     /locus_tag="PLG98_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOE32144.1"
                     /translation="MNDYKTSSTVLLGIINAALKMGVDIQSLLRQLGISINLDHISQS
                     TINLKQVHALVAAVEKAGGHPALGLLTGEDFDFEYLPDLKSFLMSASTLREAFESIRP
                     IQKLISPLLILNAEETGKVAIIKFQPDGMLDGEDERHYSEMIFTCIKTIVNRLMKKTV
                     LPKAVYFRHGRSELTPLYEDFFGSVIVLNAPENAIIYDRTIMDLPLPGGFPEIRRQAG
                     SIVNQQLAGSPLSGGLLEDLERLLAARGELLNAPIKQVARSLNMSTRTLQRRLAENGH
                     SITGMRDLIRFKLAIQALQSKRHNIEEIAEKLGFSDRHCFTQAFKRWSSLSPSAYRKK
                     ISIA"
     gene            23685..25130
                     /locus_tag="PLG98_02475"
     CDS             23685..25130
                     /locus_tag="PLG98_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOE32145.1"
                     /translation="MKSNESTEGKSSMNPKHAPIFEPITINGCEIKNRIVMSPMNCNY
                     TAPDHYISRQQMAYYAARAKGGTGLIITEAQAVSTLPCADTYRKYNNPYLTDYRYVPL
                     QSELVEHVHSFGAKIFAQLFTGPGRQGSHDIGAAEPASASAIPWRWQFHKAMNGSMNM
                     KVLKGGIRMSGYHGAIPDIEKDPEAFIALVDKIPMMHMMGRPPREITREEIKQLVIDE
                     GKGARLAKLAGYDGVEIHACHGYLVHSFLVQRSNFRKDEYGGSFENRIRFLLELVRSV
                     RRTVGPDYPVGVRFSASDDLPDGYDPNVAAMIAKRMEEEGADFIDLSDGSYEAMSDFL
                     PNTEGQVMDKSAVIQAAVKIPVMCPSVHNPDNVADVLQKGKAQMVLQGRQQIADPDWV
                     NKVKEGREKEIIKCTRCNLGCLIRFVAMLPVRCIKNPRTGLEEHIEEYTRRPILPLKN
                     RVWQTVADWGRKEPSETWEYTNEEIEQIMNV"
     gene            25198..26226
                     /locus_tag="PLG98_02480"
     CDS             25198..26226
                     /locus_tag="PLG98_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE32146.1"
                     /translation="MAKINDDGFFKIDPEPHLIGKNGSYYEYFPGYKMWWKAIDNISR
                     PLFSCTHPTIMAKAYGGLEEWEALKNPDPSNILHAMDKYGVDIACLLPESCMDTCGYS
                     GRFCTNGEMAEIVESNPDRFMYQPNMSPIKFKGVKNSIRELEYWVKEKKAKIFKYLCT
                     EDTYINDPDLWPFYEKCEELGIVLDIHTGMSWCPPQASKYANPVFLDDVCRDFPDLKI
                     IAFHMGYPYCDQLNMVAMGHANLYVCLSLLVPWAITAPRKFAAIIGEALRWVGPDKII
                     WGTDYAGFALQIKNAVEGLRDFQIPEDMQEGYGYPPITDEDRRKIFGGNLGRLLGIDT
                     TKRRVKST"
     gene            26558..27121
                     /locus_tag="PLG98_02485"
     CDS             26558..27121
                     /locus_tag="PLG98_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOE32147.1"
                     /translation="MSTPRKIKTPLTGEVITSLHAGDMVMLSGEVYTARDVAHRRLYE
                     SLLKGEKLPIDLSQAVLFYAAPTPAPPKKIIGSIGPTTSYRMDFFTPKLIENGLRGMI
                     GKGNRSPEVVEAIKKYKSVYFGAIGGIAALMSQCVKKVEMVAYEDLGPEAIRKLTVRD
                     FPLVVVNDSSGKDLYVSAKQKWHISLI"
     gene            27220..27768
                     /locus_tag="PLG98_02490"
     CDS             27220..27768
                     /locus_tag="PLG98_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOE32148.1"
                     /translation="MIEVRWHGRGGQGAVTSVELLAQAAIAAGKYAQGFPSFGPERRG
                     APVAAFTRVADKKINVRSGIYEPDVVLVLDSSLIGLVNVLDGLKPGGKLIVNTHKTPE
                     EIRKALDYKGILAIVDGTGIARKEMGVPIANTTMIGALLKVAGIMELDAMKESIEHRF
                     GRIAQKNLNAMKRAYDEITIYN"
     gene            27782..28060
                     /locus_tag="PLG98_02495"
     CDS             27782..28060
                     /locus_tag="PLG98_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE32149.1"
                     /translation="MALKTWKELPIGCVVSEPGSASEYHTGSWRSQRPIWDNAKCIKC
                     GICYVYCPEGCVQQTEDGFFQADLDYCKGCGICAHECWPRAIKMLEEE"
     gene            28062..29237
                     /locus_tag="PLG98_02500"
     CDS             28062..29237
                     /locus_tag="PLG98_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOE32150.1"
                     /translation="MGKRIGMEVAIAVAEAVGLCDIDMAAVYPITPQSHIAEHLSDLV
                     ANGEIDAEFVTVESEHSAMSAVIGASGAGARAFTATSSQGLMLMHELLPIASAMRLPI
                     CMAIANRAVSGPLNILNDHTDIMPQRDSGWISLFVENGQEAIDMSIIAFKIAEHKDVM
                     LPVNVNIDGFQLTHMVEPFEMPSKEEVDRFLPPFVPHATLHPAKPVTMGAFAMSDYFT
                     EIMKAKDEALKNSKKVIANVLDEWGKMFGRNYKLVETYKSEDAEVIMLTMGSMGETAQ
                     MAIDDLRAKGVKAGLVKLRLWRPFPFEEIKAAIKNAKKLIVTDRAISFGGPGGPVFSE
                     IKSAMYAATQRPLIYNYIYGLGGRDVTVGDFIGMFEKVLADTEHKAVDTYEFWGVRE"
     gene            29237..30187
                     /gene="porB"
                     /locus_tag="PLG98_02505"
     CDS             29237..30187
                     /gene="porB"
                     /locus_tag="PLG98_02505"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HOE32151.1"
                     /translation="MNIVENFDLFAPRLINKKELISSGHRACSGCAEVLAVRLMCKAL
                     GENTVIASATGCMEIISSMFPTTAWRVPWIHVAFENTAAVASGIEAGLKALRRKGKID
                     DRYVKVVGMAGDGGTMDIGIQALSGAMERGHDMMFVCFDNEAYMNTGIQRSSGTPMGA
                     STTTAPAGKVSSGQKTWKKNMAEIMVAHEIPYVATVCPSYPLDFIRKIEKAKSIKGPS
                     YIHCFSMCPTGWRSPSHTAISMGRLAVETGVFPLFEVEHGHYRVSPDMPKKLRPIREY
                     FKPQGRFRHLKDNEIDQIQERVSMEYQKLMEKAKYLKTGN"
     gene            30226..30705
                     /gene="nuoE"
                     /locus_tag="PLG98_02510"
     CDS             30226..30705
                     /gene="nuoE"
                     /locus_tag="PLG98_02510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOE32152.1"
                     /translation="MTKASSIAAKTKEIVKKHGKDKSALIAVLQDIQESFNYLPKDAL
                     KTAGKALGVPFSRVYEVATFYTAFSLKPRGEHIVKICMGTACHVRGAAAIQDKMERTL
                     CIKPGETTPDNKFSLETVNCVGACALGPVVVIDTEYHGQMTMNKVDKVIGKFKGEGE"
     gene            30708..32555
                     /locus_tag="PLG98_02515"
     CDS             30708..32555
                     /locus_tag="PLG98_02515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE32153.1"
                     /translation="MIKIQNAQVLHQIQEDLKRSYDPNQKIITICAGTGCRGYGCIKV
                     KDALEAELSKHNLNVEIRATGCFGFCEKGPLVVIYPEKIFYQQVKVNDVKDIVEKTVA
                     KGEILEKLLYRDPNTKETVLKEEDVPFYKKQLRLVFGSNGIIDPTRIESYITIGGYSA
                     LAKALTEMNPEGIINEIKKASLRGRGGGGFQAGTKWEGCRKAHGSPKYIIANGDEGDP
                     GAYMDRSLMEGNPHSIVEGMIIGAYAIGASHGYIYVRNEYPLAVIHLTMAIESAREAG
                     LLGKNILGSGFDFDISINKGGGAFVCGESSALFASIEGRAGEPRAKYVHAVEKGLWDK
                     PTVLNNVETWANVPLIINNGADWYASIGTEKSKGTKIFSLVGKINNTGLVEVPMGMTL
                     REIIYDIGGGIPNGRKFKAVQTGGPSGGCIPESLLDLPVDFDKLYEVGSMMGSGGMIV
                     MDDRSCMVDVAKYFLSFLQEESCGKCVPCREGIRRMREILEDICAGKGQEGDIELLEK
                     IATGVADGSLCALGSSAPNPVLSTLRYFRDEYDAHIKEHRCPAGVCKALITYSIDAGK
                     CTGCTLCSKVCPVQAISGEKKKAHTIDNDKCTRCGACFESCKFDAINVK"
     gene            32570..33283
                     /locus_tag="PLG98_02520"
     CDS             32570..33283
                     /locus_tag="PLG98_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOE32154.1"
                     /translation="MVNLTVDGKNISVPEGTMLLEAIRGAGIAVPTLCAHEAVSRSGA
                     CRLCVVEIKKGNRTRIVTSCLYGAEEGLIVDTKSDRVLNVRRLVMELLLARCPESEVL
                     QKLAKELCVEPQARFIPDTDKGKCILCRSCVRVCEEVVGVNAIGLFSRGSHKNVGTPY
                     SEKSDACIGCGACAYVCPTGHIEMTSTGDKRKIWGRTFKMQACSKCGKYFAPVDQLKF
                     ISKKTGTPLAELTICTECR"
     gene            33461..33907
                     /locus_tag="PLG98_02525"
     CDS             33461..33907
                     /locus_tag="PLG98_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32155.1"
                     /translation="MAKIVLSLVPLSGEIVKLLIRQTPDVPDFEVIAGYEMSVDEIKI
                     NLSRADIVLGDYTFQHKITADIVAEAKSLKFIQQPSVGYQHIDVEACTARRIKVANTA
                     GANAVSVAEHAIAWGLCLIKNMFYVHTSTRSGADLQRRSRCSAGNP"
     gene            33900..34310
                     /locus_tag="PLG98_02530"
     CDS             33900..34310
                     /locus_tag="PLG98_02530"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE32156.1"
                     /translation="MRDKTLKEGEVIVIRYEGPRGRPGMPETLAVTMGLDLAGFTNVA
                     LVTDGRFSGATAGPCIGHVSPEAASGGPIVVIEDGDMINIDIPARRLDVDLKPQEIAR
                     RLAGWKPLHKQITPGYMRRYVRMVGSAAYGAVLE"
     gene            34502..35668
                     /locus_tag="PLG98_02535"
     CDS             34502..35668
                     /locus_tag="PLG98_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32157.1"
                     /translation="MSFNERTYVATDSLCPPVANQFVFDGEGVLDPYLWRTAVAVASE
                     ANPGARLILKGHLAWSRWIDSGNPPRVREIDGSRWDGHGPEGAPVLLEPLPFRESPIC
                     EVILVHGPVPRVVFRTHHGVMDGRGTLCWAEDVFRALRKENVMGSTSTLTEVQMAESF
                     QKQFRRAFPVEHIAPTGLPEGDARGVIWKRRSIKGKVPLLLPRCARFAAEEAWRHAEG
                     IVRFAVAVDMRPRRPELRSTGNLTLDLYVEINKNTTPELIADDMAYQLRHGFEGRIKK
                     YDQLVKYIPLRLVRFGAQKMIRDRHRKGRYGLSGIFSNLGRVDLNFFCGGGFSARSFW
                     AVPPATEYFPFFLVLSGYEGGSELVLSVPKNLGSAGRLDDTLECIARKLEQVTR"
     gene            complement(35665..36495)
                     /locus_tag="PLG98_02540"
     CDS             complement(35665..36495)
                     /locus_tag="PLG98_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOE32158.1"
                     /translation="MALSGFRRQSIFALFFLLLVLPDTASAVNFYDGARAKQGTYFLT
                     YTSVYAADKTTNGKGNHASKDMGLLNAQELLRLCYYSPDFVATALVPFGLMKIDSLDQ
                     ESSGIGDINLGAGYFLPIRNVDILPMIFVEYPTGQYSSAKSANIGSNQLDIKPVVFLH
                     KSMGDFSIDAAVKYVFRFETERTDVLPGDEFHLQCLLGYHLTEKFKLGPSVNWLQSKE
                     KKQNGTAVDDSARAVLSVGADIYYRFSRFGITLTYLCDVYTENSAQGHFVQIKTVYRF
                     "
     gene            36716..38554
                     /locus_tag="PLG98_02545"
     CDS             36716..38554
                     /locus_tag="PLG98_02545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE32159.1"
                     /translation="MWKDKVVSPSEVLSQIEPGMTIFLGTGMAEPRTLVKNLMASEEP
                     NLQDLELIQLVSIGDTIPIDERYSRKFRLRTFFSGWIASEAVSAGRIDLIPSRFSKIP
                     QLFKSGAIHIDATFIQISPPDENGYACLVGVDVERQAMETAHLVVGEINAKAPRIMGD
                     TLVHMDEFNFFVESAEPPLYIPRWPVEDVFLKIAANIAAVVEDASCVSFGIGPLYEAL
                     AVQLTTKKNLGVHSPFFTDSLMDLVQSGAVTNRYKGVFRGKCAASYLLGSEKLMRWLD
                     RNPLVEFQPEDVITDPKIIGTNDKTISILPARKIDLTGNVALHTGKGNVTAGPGNVQE
                     LFIGASLSKNGRTIFGLPSRNRKGLPNIVLSVDNLPFQFTNRESMDLVATEYGVAYLM
                     GKTMRERAQELIDIAHPDDREELVRQAKEAKLLYADQIYFPESGHLYPARFICSHEFK
                     GGLKVLFRPIKPSDEEKMRDLFYRFSDQAVYSRYFTSIKTMPHKKMQEYVNVNYRWVM
                     SIVGVVEAAGTEKIIAEARYARTKQDAFADVAFVVDEDYQNMGIASYLFELLIRAARE
                     EGIKGFTADVLASNKSMLKVFEKAPFPVQTVLSRGIYELTIPFADA"
     gene            complement(38587..39732)
                     /locus_tag="PLG98_02550"
     CDS             complement(38587..39732)
                     /locus_tag="PLG98_02550"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE32160.1"
                     /translation="MISKRASKFTSFIVMDVMAKAEELERQGEKVIHMEVGEPDFPTP
                     PVIMKAARDALAKNKTLYTHALGLLELRQAICDFYFLEYGVSITPDQVLVSTGTSPAL
                     LFAMLAILDPCDEVIISNPRYPCYQNFILAAGGKTREVRTYPEDGFQYRPKDIKKKLS
                     SRTKAIMINSPSNPTGIVMTEEQLQNVAQFDKQYIISDEIYHGLVYKDRAHSILEFTD
                     KAFVINGFSKLYAMTGWRLGYCIFPKTFSKVMQRIHQNFMISANGFIQWAGIAALTKS
                     QKDVARMVRIYDERRRYMLSRLQDMGFVIHVEPTGAFYVFADARRFCKDSYKEAFRIT
                     EEVKVGVTPGIDFGSGGEGFLRFSYANSLENIKEGLNRLEGYLKKRR"
     gene            40050..40853
                     /locus_tag="PLG98_02555"
     CDS             40050..40853
                     /locus_tag="PLG98_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE32161.1"
                     /translation="MRTVLIIILIAVSSLGGCNMQNRFLYFPDATWPTKQALANENMN
                     FWQATGDDYQGLVSAIDAPAPNGTIVLFHGNGGTALDRGFYLTPFMNLGFRVILAEYP
                     KYGGRPGKVGEKPFVAAGLETVRLAREHYGDPLYLGGESLGCGVVAAIVRQTPVPVAG
                     IMLITPWDTLASVAKSLFPFFPVKLVLTDQYDSMENLKTFKNKIAVVGAERDEILPVK
                     HAINLYNALPEGRKRLWIIKGAGHNNWPMFTDVSLFREIVDFVKIDVHQ"
     gene            40908..41909
                     /locus_tag="PLG98_02560"
     CDS             40908..41909
                     /locus_tag="PLG98_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE32162.1"
                     /translation="MKQEKNLKIGRRSFLKLTAGAAALSGMSLPKKSFAGTEQRLSTL
                     IDLSLCDGCVDREIPACVGACKEINRDKIPQVIDPIPEPWPRKTIEDWSHKRDVADRL
                     TPYNFIYVHKADVRVAGQAKTIFVPRRCMHCDNPACATICPFSANHKHKSGAVVIDQN
                     LCFGGAKCRDVCPWEIPQRQSGVGIYLNVLPGLMGNGVMYKCDLCVDRLTQGKLPGCI
                     EACPREAMLIGSRAAIEKAALSRAARINGYLYGKTQNGGTATLYVSPVPFEEINKTMI
                     KKPGQPDMKMNVERRMVGTDALGKAVLAAPVLGLAAAAVVGVWGRIISRKEKAGREE"
     gene            41914..42717
                     /locus_tag="PLG98_02565"
     CDS             41914..42717
                     /locus_tag="PLG98_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOE32163.1"
                     /translation="MTDKILTQENRVVRHGAMELIEHWAIALSGLILLLTGIFELPVG
                     KRYYIIDVPGLGWSADFITSLYLHYAAALVFTAAAVFHVFYHGLRGEKELLPRKGDLS
                     ESVTVIKSLFGFGEEPPMRKYLPEQRLAYAAMALIILMLIISGMVKTYKNIYAPDMSH
                     GILLWATWIHNIGFILFFLAFLAHMAAIALRPNWPMVRGIFTGRVRLDYARHRHPLWI
                     AGMEPEPQDPATVEEGEQQPLPSEEAPVMEETEEEDLPPGKPQPEEDKT"
     gene            42763..43845
                     /locus_tag="PLG98_02570"
     CDS             42763..43845
                     /locus_tag="PLG98_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE32164.1"
                     /translation="MENTPKTGYKRHTVIEEEIPIYRRISLFRVLHISALMIFILLAL
                     SLVAVSVGSVSIEPGRVLTFFFGPLTGQSPLSSEEELIICSVRLPRIVFAGIVGAALS
                     LGGVVFQAILRNPLADPYILGISGGAALGAIIGIVIGAGAFYLGIPLLAFAGALATVF
                     IVFMMAGKTRGPLLDNSLLLSGVVVNAFFSAAILFFLSIVNSMELHSITFWLMGDLSH
                     ASAREILVAALCLLSGFVLLYAQARKLNLIVQGEETAEHLGVHVARTKKWLMVITSLM
                     IAVAVSLAGIIGFVGMMVPHLMRLVFGSDHRLILPVCALFGASFLIAADTVARVLLAP
                     AELPVGVVTALCGAPYFIFLLKRKRL"
     gene            43848..44627
                     /locus_tag="PLG98_02575"
     CDS             43848..44627
                     /locus_tag="PLG98_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32165.1"
                     /translation="MSVITAENIRFSYPGKPVMNGVSFSIEAAEMVAVIGPNGSGKTT
                     LLKMINGTLFPDGGRMLIDGRETTGWPRKEMARQVAIVPQETAVTFPFYAEEIVLMGR
                     FPHLNNYRFEDKKDYRIVREAMEKTDTISFAARRLDELSAGERQRVLIARALAQEPKI
                     LLLDESTVFLDLKHQAEFLSLLRQLNAVQNLTVMFVTHDINLAAQHAHKIILLDCGKI
                     YAIGMPAEVITAANIREVYDVDVFVDRNPHDGSPRATLLTN"
     regulatory      44676..44876
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            44905..46977
                     /locus_tag="PLG98_02580"
     CDS             44905..46977
                     /locus_tag="PLG98_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOE32166.1"
                     /translation="MKKILFFVLFFSVVVFSPANASQKDADNALVTMGEIVVTATRDA
                     QEIRKTPSSVSVITEEEINDSGATTLVEVLDRIESIAFRTYSGNSSQSQIDMRGFGGD
                     NPFGKTLILLDGRRLNRTDMASINWLQMPVNGVEKIEIVRGPGSVLYGDAAVGGVINI
                     ITKKGKGKPTVNLSAIGGSYGLHDERIGVSGATGKWTYALTGENNFSSGYRDRSKYSA
                     RGGGLDVGYFAGDILNMSLGVSFNRVDYQMPGALTKAQMVKDRRQYQPAMPDYYMNAN
                     DDNDGSDKYTNIKFDVKSFWGAWGRTEVNFLYGKKDLQMNMPSWFSYNYSDTDAETFG
                     MTPRYILEKNIFGFHNKVIMGLDYYHEPYAKDIFSNRRRTDRLSTADFTRDALGYYIR
                     DEFSVLKNLILSAGYRFDRVSVEGSNEDFSTPSNNFTNQKNIYKAEAYEAGLTWLWGD
                     QSKIFAKYATVYRIPFLDEVASFSGFGGNFLKSLEKEKGKSMEMGAEYYLLNNLKLGV
                     ALFRLDMEDEIEYVYDPETFTGENRNTGKTRHDGVEASFAYLLQKYFRIFGNYTYHKA
                     TFEKGLYNKKEMPMVPRHAANVGLEIYLPGHVTLRTEVQHVGKAYLSGDSDNSTEKLE
                     DRTLLNIVLQYKPVWDTMKLTAFLGVDNVADVKYSSFGIDYEQYAMPNFYYPMPGVTF
                     KGGVSVEF"
     gene            46967..47884
                     /locus_tag="PLG98_02585"
     CDS             46967..47884
                     /locus_tag="PLG98_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOE32167.1"
                     /translation="MSFKHLSFKDNAVIVFSVLILAVFCAGLACARTVTDEMGRIVHV
                     RRVPQRIVSLAPGITETLYALGLEDRIVGVTTFCDWPPKARLKGKVGGFTNPSIEKIV
                     SLRPDLIIATADGNKKDTVEKLERLGLPVYVTKPSDTRGVLESIMRIGEITGRTKEAD
                     ELADGLRKRMNRITARIRGKNRPRVFFQLGMDPMITAGRNTLIHEVIEKAGGINIAGH
                     DTANYPRYSTEGIMAASPDIIVFAPMVNDQEFSIVKKFWGRFGKIPAVQNNRIYLIDA
                     DLINRASPRIFDAVERIALIFHPDIKCFQ"
     gene            48004..48540
                     /locus_tag="PLG98_02590"
     CDS             48004..48540
                     /locus_tag="PLG98_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOE32168.1"
                     /translation="MIDGKLPTRGLVYAALFGAMTAAGALIVIPLPPVPVTAQTFFMN
                     VAALLLGGPLAVVSQIIYVLLGVVGMPVFAGGKAGLGILFGPTGGYLFGFIIAAFVIG
                     HVHRMKKNAGAVWQVFSMVLGMLVIYVAGSIQLAWIANMSLPQVLIVGVVPFIPGDLI
                     KIALAVLISSRLKNRIEI"
     gene            48551..49384
                     /locus_tag="PLG98_02595"
     CDS             48551..49384
                     /locus_tag="PLG98_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE32169.1"
                     /translation="MIVLEDVYCRYEPEGIEALRGVSLQIREGERLALIGPNGCGKTT
                     LIRHCNALLVPEKGHVTVGGLRTDHKENQPDIRSRVGMVFQNPDHQIVGMTVEDDVAF
                     GPENLRLPAAKIRARIAESLSRVGIYGLAARNIEDLSGGEKRLVAIAGVLAMRPRYIA
                     LDEPTAFLDPAASGRVLAIIDKLHAEGVGIIHITHNMAEASRAQKIVVMDQGQICMQG
                     DPEEIFSRFEILQKLGLQLPQITELLIRLKQTGFPVRTGIVHMEDALKEIISMTARRK
                     D"
     gene            49387..50184
                     /locus_tag="PLG98_02600"
     CDS             49387..50184
                     /locus_tag="PLG98_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOE32170.1"
                     /translation="MYRTGSFIRGESFIYRLDPRLKLAVTVLLSLLIFQVEQPLVFAS
                     GIGLFLLAMASGIRLRTIGESIRPVLFFIVLIFLSHLLFSDDKRSMMTIPFLGIAFSS
                     AGLKQGTLVVGQFLCLIVGAVLLTMTTAPSQIIAALRYFLKPFKKLRLPVDDVAVMVA
                     LALRLMPLLLMEKERIDAARKARGFCLRRQSVAGRMNSFLSLISKMLLGIFRKADDIA
                     LAMEARSYRRGGRSSFVELKFSSADYVVLVFLAIFLVIFVALNSHFG"
     gene            50213..51040
                     /locus_tag="PLG98_02605"
     CDS             50213..51040
                     /locus_tag="PLG98_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOE32171.1"
                     /translation="MVDTQNKLKRAVADFERAKILSIDTEYDSFHYFREKLCLIQIHA
                     NHTTYLFDPLGDVALSFLGDFFDNQRIVKILHAADNDIRLLRRDYGFGFRNIFDTHRA
                     AFLLGFRELSLDKMIKEFVGVELKKNKKIQRSHWDNRPLTEEQVEYAVQDVIYLPDLY
                     QKQTAELTAKNLQESAREAFARIAETDWREKKFNRWGYAKIKGYNALNQEQKDLLKKL
                     YLWRFHRAKEENRAVFMFLPDKNLLDLALDADHPEKYLSQRKMRIYGSEIETIIKRM"
     gene            complement(51213..52001)
                     /locus_tag="PLG98_02610"
     CDS             complement(51213..52001)
                     /locus_tag="PLG98_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32172.1"
                     /translation="MKKLAMTQEKTASLMMLVMFLMIVFIGACATTPATRAVWSKPNA
                     AQQDFAKDMDDCMKKAQATGSASTNVDMYNRCMETRGWTLKEVKSVSESSGITERYTF
                     ADGTRYEGTMANGKPHGRGTYNWTNGNKYEGDWFEGKMSGKGKYTCKNGREFTGNFEN
                     NKPVGFTINCSGNVYYVPDSRQKAQPRHGSYTDANGNKYEGQIINGKLHGKGKITSPS
                     GNKYEGDFVNGKIEGRGTYTCSTGKTFTGYFKNNKPVGFKVKCK"
BASE COUNT        13505 a        12080 c        13870 g        12676 t
ORIGIN      
        1 ttttgtttct tttcattata tgaaaataat atggccgggt cccgcgcaac ggaggcttgt
       61 gaaccccgtc aggtccggaa ggaagcagcg gtaacaatgt ccgccatgtg ttgcggtaaa
      121 cccggtcacc agaataccaa tctgttttat gctgcattaa agttccctcg gggaaaacgg
      181 acaagatgat gtaatgagat cgcgcacccc acccccaagt cccctcgacc ctgaagggca
      241 ccgcggtctc aaggggcggg gaaattattt cggaggtttc attggaatat caggtattag
      301 cccgaaaatt taggccgcaa acttttgaag aagttgccgg tcaggcgcat gtggtcaaaa
      361 cgctgcgcaa tgccattgcc caggggcgtg tggcccatgc ttttcttttc agcggtccgc
      421 gcggtgtcgg gaaaacatcg gtggcccgga ttctgtccaa ggcgctcaac tgcgagaaag
      481 gaccgacacc cacgccctgc aaccaatgct ctaactgtat cgaaataacc aataattctt
      541 cgctggatgt tcatgaaatc gatggcgcat ccaaccgcgg tattgatgag atccgcgaac
      601 tgcgggaaaa tgtcaagttc gcgccggcgt catcccgata taaaatttac atcattgatg
      661 aagtccatat gctaaccgat ccggccttca acgcgctcct gaaaacactg gaagagcctc
      721 cggcgcatgt cgtttttatt ttcgccacga ccgagagcca caaaattccg gccacgattc
      781 tttcccgttg tcagtgctac gattttcgcc gcataccgct gaaagaaatc attgccaatc
      841 tggaactggt tgcttccaag gaaggcatca atatcagtcc gattgctctt tcctggattg
      901 ccgaagcggg cgacggcagc atgcgtgact cgcagagtat tttcgatcag gtgatttcct
      961 acgcaggact ggatgtcaaa gatgccgatg tcgaggagag cctggggctt gccgcacggc
     1021 aatatctttt cagattgtcc gaagctgtct tgaatcagga tgccggttcg tgcctcatca
     1081 ttctggagga agcctatctg gcaggtatcg atatgaagta tttctaccag atgcttctga
     1141 agcacttccg gaatctgttg ctggtcaaaa ttgccggcga taaaagcgcg tcgtttgata
     1201 ttgcgaccga gcagattgaa aaattgaaca cgcaggtgca gacaacatcg cgggaaactc
     1261 ttcagcggta tctggaaatt ctgattgcgg aagaggggaa tttccggcgc agtcaggaac
     1321 cgcgcatgaa actggaaacg atcatcgtca aaatggcgta tctggaaccg atcatgccgc
     1381 tgggtgaaat tctttccacc atcgagacca tcgaaaaaaa attacagagc ggcttgtcgg
     1441 gaaacgatga aaagacggta ccgcaatcta ttgcagcgca gggctcatcc ggcgggatgt
     1501 gctctgccgg ggcggcgaac agcttttccg gagaatgcaa ggatgaaaat cattgtccgc
     1561 ccgacaatgc ggctgatttg aaaggactgc gcgacaatct gaaaaagttt atcaagaaag
     1621 aaagtccgat tctggcggca aaaatcgagt ccgctgaaat cctccgttgc gaaaacaact
     1681 gtctgacaat cggcttcccc gcgaatttta ttttcctgga tgaaatgaaa aacgttcaga
     1741 agcagcgact ggagcagatt gccgggaaat tttttcaaca aaatatgacg cttgttattg
     1801 agacactgag tgcagaaaac ggtggagcaa atggcggcag accgcgcagc aaagccggta
     1861 tccttaacga tatcaagcag gaagcgatga aacagccgct tttgcgaaaa atcattgatg
     1921 aacttcctga tgcccgggtg attgatgtca gagtgcagac agaaaaaaca aagtcgtaaa
     1981 aaccgaatga aaaatattca ttaaccatga ggaggatgaa gcgtgcagaa ttttaacacc
     2041 atcatgaagc aggccaaaaa aatgcaggag cgtatggcca gccttcagaa agaactggag
     2101 acaaaaaccg ttgaggcgca ggccggcggc ggcatggtca gcgttgtggt caacggcaaa
     2161 tatgaaattg tttcgttgaa aattgaaaag gaagttgtca accccgaaga tatcgagatg
     2221 cttcaggatt tgattgccgc tgcagtgaac gagggcgtcc gcaaatcgca ggaaatggcc
     2281 gcggcggaga tggggaaaat taccggaggg cttaatatcc ccggatttta aataaggtac
     2341 atttatgaaa gcatatgcac aaccgattaa aaagctgatt gccgaactgg gcaaacttcc
     2401 cggcatcggc gagaaaacgg ccacgcggct ggccaattat attttgcgct ccaccgaaga
     2461 tgacgtgata aaactggctc agagcattgt ggaagtcaaa aaaaagatca aactttgccg
     2521 gatttgcctg aatctgacgg aagacgatac atgccacatc tgccgcgatg cctccagaga
     2581 tcaggaagtg atctgtgtgg tggaggatcc ggacgcgctg gcggccattg aagaaagcgg
     2641 cggttttaag ggaatttatc atgttctgca tggggctctg gcgccgctgg acggaatcgg
     2701 gccggacaac ttaaggttgg gcgaactggt tcagcgcatt aacagaggta atatcaagga
     2761 aattattctg gcaacaaaca ccaatgttca gggagagtcc acggcattgc ttttgaccga
     2821 aatgttcaaa gaaaaaaata taatcataac gcgcatcgcc atgggcatgc cgatgggcgg
     2881 cgatctgaag tatattgata agatgacgat atcgaagtcg ctggagtata gacgcggcat
     2941 gtagcatgac gcgtgaaaaa gtccggatac tgcgttgcgc ttcggcatcg cgacgtacgc
     3001 taaaagtacg cctcacttcc aatgcatcgc gtgccttgtc tctggaaatt ttttgacgcg
     3061 tcatcaaata atgtcatact ggcacaggcc ggtatccaga aattgacctg ttagaaacgg
     3121 gaatccgttt caaagagact ggattccgta tcaaggacgg aatgacaaaa gagcggcaat
     3181 catcgatttc ctaaaagtta aaaagtgtca taagggaata aggtttatgg ccactgaaat
     3241 tacaaccaaa acaaaattgc ggaagattaa cgccaagaaa atcaccaaag cagtgaagaa
     3301 gctttttatc gaagccaata ccgttttggg cgaagatgtt gttgaagcgc tgcatcaggc
     3361 actctcccgt gaggaatctt caagcggcag acaggttctg caaaaaatca tcgagaacgc
     3421 cgatatcgcc cgccgcaaaa aaatgcccat ttgtcaggac acaggtcttg ccgttgtttt
     3481 tgtggaattg gggcaggatg ttcacattac cggtatgccg ttgcatgcgg cgattgaaga
     3541 aggagtgcga caggcttacg ccgatggcta tttgcgaaaa tccgtctgcg atcctttcac
     3601 gagaaataat acaaccgaca atcttcccgc gatcattcat gtggatgtcg ttgccggcag
     3661 tcatttgaaa atcattgcca tgcccaaggg cggcggcagc gaaaattgca gcgctgcgaa
     3721 aatgctcact ccggccgccg gaatcgacag catcaaaaaa ttcgttctgg atacggtgga
     3781 aaatgccggc gccaatccct gtccgccgat cattgtcggt gtgggtatcg gcgggtcgct
     3841 ggagcaggca tgcgtgatgg ccaagaaggc gcttttgagg ccggtgggag aacaaaacag
     3901 agttgacgaa cgtgcagaac agatcgaaaa agaattactg gaaagcgtca acaacctggg
     3961 catcggtccg ggaggactgg gcgggcgtgt tacggcgctg gcggttcata tcgaaatgat
     4021 gccctgccat attgcatcgc ttccggtggc ggtgaatata cagtgtcatg tggcgcgcca
     4081 caaagagacg actttgtaaa aagaaacggc ttagtttacg gtaagcttca attttgacaa
     4141 ctcccaaaaa ctagagatca gtgtttgtaa taaacaaatc aagtaagcta atattgattt
     4201 tgtcatacga tgggattctc tgatattcta tagaaaatca tgtttatcga ttaaaaagca
     4261 aattagacaa tttaattaaa atatagtacg gtactactaa gaattgtagt atatagttga
     4321 tgttatatag atacgatata ataaatacat attaatatta tatgtttagc aaaatttttt
     4381 atgagagtaa aaacatgaac aagcatcctc ggcacatgaa aatttcagaa ctcagtgctt
     4441 tttccaaaac tccaataact accattcggt actatatctt acaaggtttg cttccggtgc
     4501 cattgaagac cgccaaaacg atggcctact atacggaaga gcatcttaac aggattctgg
     4561 aaattcaaaa actcaaaaat gagaatatcc cgctcatgat tattaaaaag aaacttgcag
     4621 acaatattaa taatgcgtgt gttgtgacgg aagatagtgg cgaattatta acaaaaacgc
     4681 gtgatgagat tataagaagc agtgtaaaac tattcaggaa gaagggttat gacgccgtga
     4741 aaatttccga tattgccagt catgccggta tagggaaagg tactttctat caatacttca
     4801 gcaacaagga agagcttttc tttgaatgtc tggagaatat cttcctcgat attggggttg
     4861 acgtgcctga aattcaatct gaaacggacg ctttaaagcg actaaggata cgcggctatc
     4921 atttctatca aaactttgtt catatgctcg atatgctcaa tattgccagg cgtgcatcct
     4981 tgatcaataa accggagtat aaagaaaaac tggagaaagc tttttttaac tttgttgagc
     5041 ctatcaggca agaaatagaa ctgattcttg aacagcgaaa cagcccatta aaaaacagta
     5101 cattggtcgc ctacctttta atgggagccg ctgaatatat gtattacttc ttacttaatc
     5161 acaagaaaac acctgaatat attgagaaag aattctggaa tttctttttt ggtagcgtgc
     5221 catttcagga aggttagttt aaaatgaaaa aaacaggagt tatgcaaatt tcagagttgg
     5281 caaaggtctc cggtgttccc gtttcgacga tccgttacta catccgggaa gggttgcttc
     5341 cgcaaccgtt acgaacgggg aaaaccaggg cctattattc atctgatcat ctcaaggcaa
     5401 tcgagtttat caagagaaag caggccgtcg ggaataaatc gcttgatgta attcgcaagg
     5461 aaatcgaaaa gaagttcccg caacaagaaa atgcctctga tgtgcaaatt cctccggggc
     5521 acagagaaaa aatcttatca tcggctacgg aattgttttc cggcaaaggt tatgcggaaa
     5581 ccagcatagc ggatattgcc agccacgcga agatcagtaa agaaacattc tatgtccatt
     5641 tcaagaataa agaggaactt tttatagcgt gcgcagacat tatctttcac aatatgtata
     5701 ataatgtgtg gcaggaaata aaggatgaaa aaaatattat tgaacgaagt aaaaaacgag
     5761 caaaagcatt cattgtttca tttccaaagt ggataactat gatgaatctt gtcagaagcc
     5821 tttctgtcgg taataatccg gtactgagag aaaaattcaa gcatctctta agccagatga
     5881 tcaaccccat tagcaacgat tatgaaagtt tcaaaagaag tagcaacatt cataaaagaa
     5941 cggacagcat cataaccggt tattttataa tgggcatggc agagtatggt gcaatgctga
     6001 ttgatagagg agtatgtact gaggatgagg ttatagatta tgtcactacc attgttatgc
     6061 aaggtcttta gtccatctaa aaaatatcta caatattaaa taaaatatat ttaacaacta
     6121 ttctatatag aaccgatagt tactgttctt tatattatat ctaaaaatat ctttaatttt
     6181 ccaattatta tctcatcaaa aaaacacttg acatttatta tatagctata tactttaata
     6241 ccaaacgtta cagttctagt agttacatat tttaatattt cctcaggaaa ggaggtttca
     6301 taacaaacat tatgagtaaa ggaaaaattg ctattattgg catcggtgag gttcccacgc
     6361 gcaacatgcc ggagcgcacg cattgggata ttatttatga cacatgcatg gaggcggtca
     6421 gggattcggg acttaacaag aatgacattg agggcgcaat cagcgtcact ccgcaggctc
     6481 agccaaagat tgccgcggag ctttcctttg gcaagctgcc tgaagaactc ggactcaagg
     6541 ggtgcaagga tgcggtcagc gtcaatgcgg gcggggcttc cacctcgaac gccatacggc
     6601 tggccgagca atggataaca agcggcatcg ccaaagcagt gcttgtacat cacgtcaccg
     6661 tgcattcgac tattccgatt cctgatctga tcaatttctt tgccagagcg ggcgttgatc
     6721 tccagtggga atatccctat ggcaccactt acaatattat tatgggcttg atggccaatc
     6781 gatacatcaa agaaacaggc tgcaagcccg aagagatggc ctcggtcgtt acggcgctta
     6841 gaagttgggc cgccaaggat ccgaattcat ttttctacgg taaggaggtc ccttcggtgg
     6901 aaaaggtcct tgcatccgcc ttactcaata cacccctgca caagcgcgaa agcaacgtgt
     6961 tgggcgacgg cggcagcgcc atggtgatcg tgtcagccga agatgccaaa aaaatgaagc
     7021 gcccggctgc ttacaaactg ggcgattccg tgcgctattt cagcggaacc tgcgtcatgc
     7081 gggatttcta caaggtgacg gaaggcttca ttgtcaccgc caaagaggcc atggcccagg
     7141 ctggcatcac caaagaagat gtcagcctct ggaactgcta tctggcctat cctctggccc
     7201 atcccctcat agcagaactg ctcgaagtag cccctcaagg tatgggcggt aaatattttc
     7261 tggaaggccg gatgtctttt ggcggagata ttccctggtc atccatcggt gacgcgccgg
     7321 ggagaggcca taccggatcg ggtgtgagcg cggccacgta cgtggaaaca gccaggcagc
     7381 tcatgggaaa ggctggcgag cgtcaggtga gaaactgcaa atacgtttac cagaatgccg
     7441 gcggcggctc gggattcaac aacattgcca ctatctgggg aaaggagatt taaccatgga
     7501 tttcgatatg acaaaacccg ttccggagac acaaccgtgg tcggagaaat tttgggaagg
     7561 cgccaaagag ggcaaattac ttattcaggt ctgtaaggac tgcaaatcaa aaatattcta
     7621 tccgaggaaa ttctgtcccg agtgctggtc cggcaacctg ggctggatcg aagccaccgg
     7681 caaggcgaag atattcacct tctctacggc ctatgacatg gtggagccta agtttacgga
     7741 tgagctcccc tatacgatcg cctatgtcga tctcgatgaa gggatcagaa tgatgacccg
     7801 gatcgtggac tgcaaaccgg aggatatcag tattgacatg gaggtggaag tcgtctttca
     7861 ggagcgcggc ggctatttcc tgccttactt tcgtcctgcc ggtaaaaagt aaaggagctg
     7921 atccatgcct gaatatacta ccatcactta tgaaatcaag ggaccggtct gtacgattac
     7981 gctgaacagg ccggacaaat acaacgccat caatcgggaa atgtccgccg aactgctcga
     8041 tgcgtttcga aagatcagag acattcccga cgttgccgtg gtcgttctcg cgggaaatgg
     8101 gaaagcattt tgcacgggcg gtgacctgag cgttttcccg tcgctggccg agcatcagga
     8161 atccttaaac tggctggctc atgatggcta tggccttggc aaagccatag agctttgtga
     8221 aaaagtggtc attgccaaag tgaccggaca ttgccttgct ggtggtctgg aactggcctt
     8281 gatgtgcgac ctgatctatg cacgtgagtc ggccaagttc ggtaccacgg agattaacat
     8341 gggaatcctg cccggttggg gcggcacggt gcgcatcgcc cgtgccatgc ccatttaccg
     8401 tgcgcgtgaa attatctaca gcggacgcaa ggatttcccg gcccgcgata tgtatgagat
     8461 gggcttcctc acccgggtat tcaaggatga tgagttcgag gccaagttca gtgaagtcgt
     8521 cacgaatatc agcttgaaaa aacctattgc tctaaggatg gggaaagagg tcatggggcg
     8581 ctcgctggag tgcggcagcc tggatgctgc actggcgctg gaacgcaatg ccatccagtg
     8641 gctgatctat gccccggata tccaatcggt gatggacact ttacgtgaaa aacccgaggt
     8701 cctggtggcc gctcagaaac aggcgaatgt cgcttcggac aagaaaaaat aaaggagaag
     8761 tactatggat ttcggattta cagaagaaca aatcatgatg cagcaactaa cacagaagtt
     8821 ttgtgaaaag gagcttgatt acaaatatgt gaaatggatg gatgaacatg tagacttccc
     8881 gcctgatgag ttgtggcaga aatttgttga tatggggaca ttctatggct ccatccctac
     8941 ggaattcggc ggacaaggac tgggacaggt cgatatcatg gtggcttatg aacagatttg
     9001 caaacgatcc atgtcggttg ccctggctat cggtgtcacg gccggttttg gcgtacgttt
     9061 catcaatgaa ttgggcaccc cggcgcagag agaaaaatat ctgccgctca tcggcgaggg
     9121 caaattcaag acctgcatgg ctttgaccga acctgccggc gggacggata ttctgggggc
     9181 aatcaaggcc acggccgaag acaagggcga caaatgggtc attaacggcc agaagatttt
     9241 catcaccggc gcgcatgtgg ccgactccct gattacggtc tgccggaccg aaaaaggggc
     9301 caagccgagc aagtcgctga ccatcatcat ggtgccgcgc gaaacccccg gcattaccat
     9361 ccgtaagatc gggaaaatca gctgtcatca ttgtgaaagc gtcgaaatat tttacgataa
     9421 cgtggaagtt cccaaagaaa acatgctggg cacgcgcggg aatgccttct acgagatcat
     9481 gactgtactc aacccggagc gcatcggcgt ggccatgatg ggtgtgggga ttatctccgc
     9541 catctatgat ctctgtttca agtatgtcca ggaacgcatc gccttcggcg ggcctatcag
     9601 ccggtttcag gtcctccaga actatctggc ggacatgtat atcaatctcg aaaatgcgcg
     9661 gaatcttacc tataagtccg cgtggctgtg cgatcacgat aaaccgtttc atctggaagc
     9721 gaccatggcc aaactggtgg cggccgaggg tgcgcgtcat gcggcaactt ttggttcgga
     9781 aatcttcggc ggttacggca tctgcaatga atatcccgta tcgatgtttg tccgggatgc
     9841 ctaccagatt cagttttcac caatatctaa cgagatgagc cgcaacgtgc tcatgcagtt
     9901 ccagggtctg cccaagtcct ggccgtgaaa ataaaggaga gataacatgt acacattagg
     9961 tgatatcccg cgatgcggcg ctgtactgtg gccggataat attgccgtag tctttgaagg
    10021 gaaacgatat acttataaac aattcaatga gcgaatcaat agatttgcca actttcttat
    10081 caaatcaggc tgcaagaagg gtgacggact tgccgtcatg gccgataatt gtgccaaata
    10141 tctggaagtc tattttgccg cagctaagat cggcatgcgt gtgacaccga tcaatgtgcg
    10201 tttaggcgac gacgagatta tgtttattgt taacgacagc gagacggttt tctttattgt
    10261 cggcgacggg tatgaagaaa aagtcaaaca aatgaaaaat cgtttcaaga gtataaaggc
    10321 gtgggtgagt ctggataatc ctgtctgcgg atttcttgac tacgataagg tgttggacat
    10381 atcgccggct ttggaaccgg atttagatca atatgatatc caggaagatg atctggccat
    10441 tctgatgtac accggcggaa ccacgggact gcccaaaggc gtgatgcttt cccaccgcag
    10501 cgctctttta gccggtataa cggctacgat agccaacgga tttacggaag atgattccac
    10561 ctgctttgtt ttgcccatct ttcacgtttc ctggtggccg attctggcga tattaatcgt
    10621 cggaggcaag gtctgcatca atcgcaaacc cagtctggac acgattttta aattggttga
    10681 tgacgaaaaa tgcacccaca tgaatcttgt tcccacgatc tacggttgga tggtggattg
    10741 gccaagtatc gtaaattacg atctctccag tttaaggatt ttaacctatg ccggcagtcc
    10801 ctttcccacg gaagttttaa aaaaatgcat tcgaaaattc gggaataaat tcgctcaggg
    10861 atatggcgca acggaaacag ccggagccgg catatccggt ttggcgataa aagagcacca
    10921 actggatggt ctgaaacaga agtatcttgt aagtgccggt aaaccgatgc ccaactcacg
    10981 ggtcagaatt gtggatgaaa atgataatcc gctgggccct ggtgaatacg gcgaaatatg
    11041 tgccaaaggc aagcatatca tgatgggtta ctggaagaat cagaagctga ccgaggccgt
    11101 cctgaaaggc ggctggtacc atacgggcga tatgggttac atggatcagg acggctatat
    11161 ctttatgacc gaccgcaagg cggacatgat tatttccggc ggcgagaatg tttatccgaa
    11221 ggaagtggaa gacgtcattt accaacatcc cgccgtaagg gaatgcgccg ttgtttcagc
    11281 gcccgatgca aaatggggcg aagtggtgca ggccgttgtc gtgctccatc cgggcaaaga
    11341 agcgacagcc gaggagatca tcgaacactg caagaagcac ctcgcgggat acaagtgtcc
    11401 caaggcggtg gcgttctgga acgatattcc caaaacaatc atcggcaaga tcgtcaaaaa
    11461 agacatcaaa aagaaattct gggaaggcaa agagagaatg atcagttgaa aattattata
    11521 aacagggggg atatatatga gcaagaagaa tgtagataat tattttaaaa tcgatccgga
    11581 atgtcacctt atcggcgaca tggagcactt gaaccatttt cccggagttc aaatgtggtg
    11641 gcgggcgatt gaacatattt cgcggccgct gatcaccggc gcacccctgc cgctcatgat
    11701 gaaggccttg gagggtttgg aagaatggga agcggtgaag agtggcgacc cgcaggttat
    11761 ccttcgggtc atggataagc acggcgtgga catcgcctgc ctgcttcctg aatccatgat
    11821 ggatacgacc ggttttagca gccggtgggt ttccaatggc gagatggccc gggtggtgga
    11881 aacccatccg gaccggttca tgtaccagcc caatatttca ccgatcaaac agcgcggagt
    11941 taagaacgcc atctgggaga tggagtattg ggtcaaggag aaaggggcca ggatattcaa
    12001 gtattattcg cccgaagata cctacatgaa cgaccccgca ctgtggccgt tttatgaaaa
    12061 ggcacaggag ttcggcattg tgctggacat gcataccgga ttctgctggg tgccgccggg
    12121 caagagcaag tattgccatc ccgagcagct cgacgatgtg gcgcgcgact ttccggaact
    12181 caagatcaat gctttccaca tgggctatcc ttacagtgac gtcctcaaca tggtggcctt
    12241 gggccacccc aacgtttaca tctgcatgag tctgctcgtt ccctgggcca ttaccgcacc
    12301 gcgcaagttc gcccaccttc tgggcgaagc catgcgcttt gtcggaccgg atcgcatcac
    12361 ctggggcacg gattacgccg gatacggcgt tcaggtggcc aacgcggtca agggtttgtc
    12421 tgagttccag attcctgaag acatgcagga ggcgtatggg tacccggcca ttacggaaga
    12481 agacaagacc aagatgttcg gcggcaactt aggccgtctt ttggggattg atacgacaaa
    12541 aagaagaatt aagcaaaact tgaagggggt ataagttatg actgaaaaat cagagggcaa
    12601 gaaacaattt gcacacattt taaagccaat taaaattggt ccgattgagt tgaagaaccg
    12661 gattgttatg gcgccgatga acgaggcgtt atcaggggct catggtgaag ttggagagca
    12721 gtatattgct tatttcggag cccgggcaaa aggcggcacc ggattgatca caacgggtgc
    12781 gatcatggcc accaaactgg cgggagagtt cccggccgga aggaatccgc atctctacca
    12841 tcagggacat atgcttggaa tgaacaacct gaccgacagg gttcactatt tcggatccaa
    12901 agttgcggct cagatgacca ttggttttgg caggcaaggt cattcctatg atcaccataa
    12961 gctggccccg gctgcgacgg ccggtctgcc ttatgagata gcagctgaaa aatcgcccaa
    13021 ccattgtgcg gatctttatg cggttatgga attttcaaga tattatatga aggggcaaat
    13081 gacgcgagaa atgtccatag aagagatccg cagcgaacag aaggaatttg ccaatagctg
    13141 ccagcttgca gttatctccg ggttcgatgc catagagatt cacggcccgc acgggtacct
    13201 cgagcatcag ttcttatctc ctttctccaa taaacgcacg gacatgtacg gtggagaatg
    13261 gcgcaaccgc aagcgtttcc ttatggaggt tgccgaacag gtccgttatg cctgcccggg
    13321 cgttgccgtc gggtgccgga tcagcgcgga agagcatatg cagggcggcc tgaccgagga
    13381 agagatgatt gatgtggcca aggatttgca gactataggt ctggattata tcagtttgtc
    13441 cgacggcgct ggctacgaag agtccggcca cctcatcacg gacatggacc gttccaagca
    13501 tattcccgag catggcgagg cattcaagaa ggcgcttaat attccggttg ttgtgtcttc
    13561 tcaacacgat ccggtcaaga tcgaaaagaa catcgcggaa ggcaagtacg acctccaggc
    13621 catgggaagg cagctttact gcgatccgga atatgtgaac aagcttgaag ccggaaaagc
    13681 aaaagagatc gtccggtgct cacgatgcaa tacctgtctc atgcgctttc ttgccgcgct
    13741 tactcccgca tgcccgcaaa acccgaacct gggcagagaa tatgccctgg atgaatacaa
    13801 aattggcccg tggcagaaac atgagtccat tatgcccgag ggcacaatga gagcctctat
    13861 gccgtcactt gatcgtccct ggtggaggaa cgaattaggc tatatcgaaa agtgctggag
    13921 accgttacag ggacgaacgt cgcgataaat tttataacta tacttttgta tgaataatta
    13981 cattgcagag cgttggcgag gatgattttt gaattgtgtc gtcgaaatta ttcttcctga
    14041 taaaaatcct ttctaatgat cccttcctgt tctttgatct ccctcttgca atgagaggga
    14101 gatcttatcc tcgggaagga aattattaga atgaaaaaga agagagatta attgaattaa
    14161 aaaagtagca agcagacatg tatatgacta ttaaacgatg tgttttcgtg agtagtgcgg
    14221 catcgcgtat gggcttggct gcggcgctat ggcttgccaa taggagtaca gatatggcgg
    14281 ttaagggatg tattcttgtc accggcgcgg catcaggcat tggtaaggcc acggtgctca
    14341 cgctcgccaa aaagggcttt ggcgtttacg ctacatatgc cacggataaa tcccaggcgg
    14401 aacttgaaaa actccgcgag gtaggtggga ttgaggcttt caaacttgat atcaccaacg
    14461 atggtgacgt agagagcgct gttgctttta ttgaggagaa gggaattaat cttcttgcgc
    14521 tggttaacaa cgcgggcatc ttcagccccg gtccgctcat ggagtatccc gtcgaacggt
    14581 tgatcgaaca atacaacgtc aacgtgttcg ggacgcatcg cctgaccagg gcccttttcc
    14641 ctctgctctt aaaatccagg ggccggatcg tgaacgtgtc gagcgtggcc gggtttgtgg
    14701 ccacgccttt ttcaggaccc tatgcttcca gcaagcatgc cctcgaaggg tggtcggact
    14761 ctctgcggcg tgaactcatg ccatggggtg tcaaagtcat tgtggttgaa cctgctctga
    14821 tcgacacccc gctgtgggat aaggacctcg aaggaagaat agagcggtat cgcggcagca
    14881 ttttctatga agccaatcgt aaaaaactcg agcatgaggc agccgaagcc aaaacgcgtg
    14941 gtgtgcagcc ggagatagtg gccgaagcca ttcatcgggc cgtgactgca ccgaatccca
    15001 agccccgtta tcttgtcacc aaccatccgg tacagcaccg gatggtgaag attctgcccg
    15061 acacgcttct ggatcggctg gtcatcaagg aattctagtt tatgaagatg tattttctct
    15121 ctactggatt tggacatgta gcgaaggggc tttttgttca gggcgcgggc atgaagagtg
    15181 tgcagtttcc catcctcagc gttctgattg agcgcgccaa tgatttggtg ctttttgata
    15241 cgggcatcgg cacgcgcata gaagaagaga tgcggcctcc catctattgg ggcaaccttt
    15301 ttttccaccg ctgcgtcatg cgtacgcggt tcgatccgca gcacgacgct ttagtgcatc
    15361 aactgtcgcg gctgagcttc cagccttcgg atgtcaagaa cgttatcatc tcacatctgc
    15421 actgggacca tgccggcggc atgcgtgatt ttccccatgc ccactatttt gtcggacgca
    15481 aagaatggga ttttgcctcc cgtctcacga atcgggcgct gttcaagaat gccttcatca
    15541 aggagcagtt ctgcggggca ggtctggata ttgagctgat agaagccgat cctcgcacac
    15601 ccttcaagaa ctttccggca tcgtatgacc tcttcggtga cggttccatg gtgctggtgg
    15661 atttgccagg ccacagtcca ggcctcatgg gtatgtacct gaccatgcca tccggcagaa
    15721 agttcttctt cagtgctgat actttctatt ttcctgaagg actggaacaa agaatgccta
    15781 aatccaagct tatgcaatcg ttggtaagcg agggtccgga atcggaggca agtattaacc
    15841 gggtctatga cctcatgaaa tccgatcctc aactggagat cgtgggaagt cacgattatc
    15901 gcttgccggg acgatatgaa ctggcagcaa acttctatga atagtgccgg gttgatcgat
    15961 cgcatcaata aaggaaagac atacatatga acctcaaagg aaaagttgct ttagtcaccg
    16021 gcgcgtcgcg cggcctgggt cagaccattg ccatgaccct cggtgaggct ggggctgatg
    16081 tagtccttac cgatatcctc ctcgaaggac agtaccacga cacagaaaag ctgggagaat
    16141 acagtctgct ggctggacat tttgcgcagc agaacacggt caaaacgcag gcgggtgcgc
    16201 aggaaatcca atccatgggt tcgcgcagcc gcgcattcaa aatggatgtg gtagatcccg
    16261 aggaagtagc gagcgtagtg caggctgtgg aagcagagtt cggaacaatc gatatcctgg
    16321 tgaataatgc tgctgtgatg gataatatgg gcaagttcgg cgaacagcaa ttagagcgct
    16381 ggcaacgcga tctcaatgtc aatctcaccg gcgcatatca ttgcagcaag gccgtctggc
    16441 cgggcatggt aaaaaaaagc tggggacgga tcatcaacat atcatccgtg gcaggcctca
    16501 tgggggcttc actgcaaccg tcgtatggcg cgaccaaggc tggattaatc ggattgagca
    16561 agtcgctggc tattgaggcc ggccgttacg gcattaccgt taatacggtc tgccccggtt
    16621 tcatcgcaac agaagcagta aaactttatg atgaacgtat gactgaacgc attgtgaagc
    16681 gaactgccct gaagcggatg gggcggccgg aagaagttgc cgaagtcgtt accttcttgg
    16741 cctcgaatgc agcgtcctat attacgggtg cggtcattcc ggttacgggc ggaatcgatc
    16801 tactcacgct ttaggaaaaa aacaaaaaga aattaaaact acatttcaga caggcagacg
    16861 caaaagggga acatgacgat gaacattatt aaaaaaggaa agggtgagtt atttaccgat
    16921 cctgatccgg accgtgcacg aacatttttc cagaagaaat ccaaaaaaat ggtgaacaaa
    16981 gctatgacac tttccaaagc catcgagact tttgttcatg acggggaata ctttgccctg
    17041 ggcggattcg gggccaaccg tacacccatt gcggcctgtc atgagatcct cagacaagga
    17101 aggaaaaata tgggttttgc cggccacacg gctagccatg atttcgagat tctctgtgcg
    17161 ggagaagtct ttgaccgggt agacgcaggt tacattgtgg ggcttgaggc ccgggggctc
    17221 tctgtctgcg cgagaagata catgcagacc ggcaaggtta aggtgaccga gtggaccaat
    17281 tacagccttt cggcaaggct tcgggcggcc gccatgggcg ttccctttat tccaacgcgc
    17341 aatctgctgg gcacggacac ctttgagtgc agtgccgcca ggattatcga gtgcccgtat
    17401 acaggcaaga aacttgccct tcatcccgct ttgtatccgg atgtgggcat catccacgtt
    17461 cacgaatcgg atgtttatgg gaatgtccgg gttcgcggca ttctcaagtc ggacaacgac
    17521 ctggcccgtg cctccaagcg ggttatcgtg acgtgtgagc gtctgattac gaatgaagag
    17581 atacgacggg atcccacgga aaccacgttc ccctacttcc tcgtagatgc cgtgtgcgag
    17641 gttccgtacg gcggctatcc aggcaccatg gcgtacgaat atttttcgga cgaagagcac
    17701 ttacaggagt ggctgaaggc cgaagaggat tctgctgttt tcaaggattt tcttcagaga
    17761 aacatttacg actgcaagga tcaccatgaa tatatcaaca agaatggtgg catggagaag
    17821 atgctccaac tcagaacgat agagcatctg ctgcataagg agggaaaaag atgacaacgt
    17881 cctataacgc gatggaattg atgatctgtg ttgcatcgag agaactcgag gacggttcca
    17941 cgctggccgt gggaaccgga gcgccttgtg cggcagccat gctggctcag aaattaaact
    18001 cgccgaatct ggctattatg tttgaagctg gaagcgtcag tccgcaactg ccggaaatgc
    18061 ctatctccgt tggcgattca cgagccagtc acaaggctgt gatggtttcc acaatggggg
    18121 ccatcatgga aacttgtcaa cggggatatg tcgactatac ttttctcggc ggtgcgcaaa
    18181 ttgacatata tggcaacctg aattctacgt acattggcgg aacttatcag aagccgaaag
    18241 tgcgtttccc cggaagcggc ggagcgaacg atttcggaag tttctgctgg aagatgatgg
    18301 ttctcacggt tcaggatgca cggcgctttg tggaaaaatg cgaattcatc accacgcccg
    18361 gctggctgca gggaggaaac agccggtatg aagcgggact acccgagggc accgggcctt
    18421 ataaggtttt caccaatatg gctgttatgg atttcgagcc ggaaagcagg cgcatgcgca
    18481 tcatttccac caatcccggt tattcggtca aggatgtgca ggacaactgc ggattcgaac
    18541 tgctgaaggc gcccaagata accgaaacat tgccgccgac tgagatcgaa ttgaaaattc
    18601 tccgggagga agtcgatcct caccgttatg ttatcgggag ataaaacaga agattgtcag
    18661 attagaaagt cgattgaatg atttattaaa cgaaagagag gacgatgata tggatcggtt
    18721 agctcaaaca ttttcagcgc tcgaagagga tcgtgtttat cagctcgtta atgaatgcct
    18781 cgaaaagtca attactccct tgttgattgt caatgaattg tccaggggaa tggaaaaagt
    18841 gggacagtta ttcaaggatg atgaatacgc cctggcggag ctcatttatg ccggtgaaat
    18901 attcaaagaa gccatggaaa aaatcaagcc tctgcttctg gctggcgaaa gcagatctgc
    18961 aggcctcgtt atcatgggaa ctgttgaagg tgatattcat gatcttggca agaacatcgt
    19021 tattaccatg ctcgagtgct ccggattcaa ggtcattgat cttggtgtgg atgtgcctgc
    19081 taaaaaattc gtcgaagcaa tcaaggaatc gggggcgagt ctcatcggca tgagccttct
    19141 gcttacaaca gccatggata gtctgcgcaa cgctatcatg gccattgaag aagccggtat
    19201 caggaacaat atcaagataa tgatcggcgg ggcgcctgtc agtgaacatc tcagggtgga
    19261 aacaggcgct gattatttag gacatgacgc tgttgaagcg ctgaccatag ccaggaaagt
    19321 ctttagtccg tcaactgtgg atgcgaggtg acccatgaaa gctgcagaaa gaatcaatac
    19381 ggtcatcaac cttgaatgtc ccgaccgtgt tcccctggct ccattgctgg accactgggc
    19441 ggcaacctat accggcatta ccaacgcgga attgatgtct gatccggaca agcgttttaa
    19501 cgcggtactt aaaacagcca ttgattttaa gtgggacatg agtttccttg ccgatacggt
    19561 caataccaca ttattaaaat tgggtgttcc ggcacggttg aagctgccgg gcatagatct
    19621 tccggaaaga agtgaacacc agttcgatga aaaagaagtc atgactgaag aggattttga
    19681 tattctggaa tctgatggcc ttatcgctct attctcaaaa ttaattccgc ggatatatcc
    19741 tgaaatgacg gttgagtcag cgatgactga ttttgcccgt gcgagcacgg aaataaccga
    19801 tcaggccgct tggctaagtg agaatggaat cgaaccggca gttgggtttg ttataccggg
    19861 tccttccttc gagtatttct gttttgcgag atccatcaat gttgcgctca ctgacctgag
    19921 aaggcgtcct gaaaaattga aaattgcggg caaacgtttc tgtcaggata tgttggacct
    19981 ggccatagca tcttccggac aaaataatat cagcagagtt tttatcggtc tgagtcgctc
    20041 atccccggtt tttctttcaa ataagatttt tgaggcattt gtactacctg atctcgaagt
    20101 attggtaaat ggtctcattg atgccaatag aacacccgtc tttcattgcg atacgaactg
    20161 gacgaaattc ttgccctact ttcaacgatt ccctaaaggc aagtgcatca ttgaactgga
    20221 tagtttcacc gacatcttca aggcaaaaga aatactgggt ggacacatgg ccattatggg
    20281 cgatgtgccc agcacgttgc tggccgaagg aacgagagat gaggtgatgt gctattgtaa
    20341 aaaccttatc gaagaaattg gcgggggcgg tggatttatt ctgagttccg gatgctccgt
    20401 tccgctcaat gcaaaggttg ataacgtgcg tgccatgtcc gaatctattg atgaatgggg
    20461 atggtataag taatgcgcaa gaacgtgttg ctaaaaatac aatgaagata ccattggaga
    20521 acattagatg aaaatcaaca tgaatcaatg gatgcagaat atcctgaaat ctgaaaagcg
    20581 aaaagccttt ccattgatgc cttacctggg gcttgaactg actggtaaga aaattcttga
    20641 ggcggttaaa gacggccaga tacaaagccg gtgtgtccag gccatagctt cccgctttcc
    20701 atcaatcgca agcgttacca taatggattt gtctgtggaa tgcgaagcgt tcgggggcac
    20761 aatacgtttt gtcgagaacg aggtgcccac aacaatcggg gctgttgtga atgacatgaa
    20821 tgatgctgaa gcattgaaaa tgccggaggt cggagcggga cgcacaggtg tttatcttca
    20881 tgctgctcaa ctgaccgctc aagcaacaac agacaggcct gttttcggct gccatatcgg
    20941 tcccttttct ctggccgcac ggctttgcgg aatgacggaa attatggtta agcttcgcaa
    21001 agaaccggtc atggtggaaa cagtgctcaa caagtgcaca gagtttcttg tccaatacgc
    21061 aaaggcatac aagaaaacag gggtgaatgg cattgtgatt gctgaaccgg cagccggctt
    21121 gatcaatgca gctcagtgcg aaaaattttc atccagccat gtcagcagaa tcgtatcagc
    21181 cgttcaggat gaaaactttt taattataat tcacaactgc ggcaatacat ataaattagt
    21241 cccggcgatg ctggcaaccg gcgccatggg tttccatttc gggaacgctg tcgacatgaa
    21301 acagattatg ccccaggttc cggcacatat tccggtcttc gggaatattg ctccgatcct
    21361 gttcaggaat ggaagcagca tggaagtaag ggagaagaca actgaattac tgatggcgat
    21421 gaaagattac ccgaatttcg tgctttcttc cggttgcgac atccctcccg ggacaccgct
    21481 cgggaatatt gacgccttgt ttgatgcatt agctgattac aatacgtgat tttttcacag
    21541 aaagtagtcc atgatgagag aaattattaa agaagatcct aaaaaaatca aaatagatat
    21601 tcaagagatt catgcttttc ggaaaagaga taaatcttct ttaattccag aaacgattac
    21661 gcgtcatgta gaaaaagcat atgacctttt ctgtcagttc gctgtcccgg tcagtattgt
    21721 gactgaggta acctactcac agtttcaatc tatttattca ggtgatggtc tgaatgagcc
    21781 gagggcgcct ctcaacgata tcatgaccga taatgctgta tatctccttt ttgcaatgac
    21841 acttggggct gatgtatcac tctatatatc tgatcttttt aaaaagggcg aacatctgtt
    21901 aggacattta cttgatgaaa tatgttcaat cggaatcgaa gtgtttgcgg atgagataac
    21961 cgggatgtgc tttgccggtg aagtctcaag ggggacactc aatcataatg acattatgtt
    22021 gctatacagt cccggttact gcggctggca tataacgggc caaaaatttt tacacaaata
    22081 cttaaaaagt caggatattg ggattagact tacagaaaac tgctatatgg aaccaatcaa
    22141 gtcaatctcg ggtgtaatga tcggatgtag tacattatca cattatttta ataacaatta
    22201 tcctttttgc atcgattgcc ggaccaagac ctgccggcag cggttggttg ttaatggtat
    22261 acgaaacagc atctgatcat cggatggacc gggatgataa ttttccatcg atggcatacc
    22321 ttctcaaaaa aagagagcac agaagcgtga tgaatatcgg gtctattcca aaacataatt
    22381 cacaacgtca atgtatcgat atgccgcggt catgtaggcc gtgtaaatat aacgatgaga
    22441 cgggattttc atgaatgact acaaaacatc atcgaccgta ttgctcggca tcatcaacgc
    22501 agcgctaaag atgggggttg acatccagtc cctcctgcgg cagctcggta tttcgataaa
    22561 tttagatcat atcagccaat ccaccatcaa tttgaaacag gttcatgccc ttgttgcggc
    22621 tgtcgaaaag gcgggcggtc atccggctct cggactctta accggcgagg atttcgattt
    22681 cgaataccta cctgatctca agtccttttt gatgtctgcc tccaccctga gggaagcctt
    22741 tgaatccatc cgccccattc aaaaactgat ctcacccctt ttaatcttga atgctgaaga
    22801 aaccgggaag gtagccatca tcaagtttca gccggatggt atgcttgacg gggaagacga
    22861 gcggcattat tcggaaatga tctttacttg cataaaaacc atcgtaaatc gcctgatgaa
    22921 aaagacagtc ctccccaagg ctgtctattt ccgccacggc cggtcggaac tcactcccct
    22981 gtatgaagat tttttcggca gtgtcattgt cctgaatgca ccggagaatg ccatcattta
    23041 cgatcgcacc atcatggatc tccctctgcc cggcggattt ccggagattc ggcggcaggc
    23101 cggaagcatc gtcaatcagc aactggccgg ctcgccgttg agcggcggac tgctggagga
    23161 tctcgaaagg ctcctggccg cacgcggtga gttattgaat gcgccgataa aacaggtagc
    23221 gcgttcgctg aatatgagta cccgcactct gcaacgccgt ctggctgaaa acgggcatag
    23281 cataaccggg atgcgggatc tgatcagatt taaactggcc atccaggcct tgcaatccaa
    23341 gcggcacaat atcgaagaaa ttgccgaaaa gcttggtttt tcagaccggc actgcttcac
    23401 gcaagctttt aaacgctggt caagcctctc tcccagcgcc tatcgtaaaa aaatttcaat
    23461 agcttaatct cgaagacata cgaactttac ccggggtaat tcaaattggc gccaattctt
    23521 cctattttgg catccgccgc tctagacgtt tccggccgta atgagatatc ggagacaatc
    23581 cttggaaaaa gagaaattga taaatcagcg ggaggaaagt tgtcagaaac gcaaatcatg
    23641 cgccaatcag tcagtctaac cactaaaaaa aggagattgt caaaatgaaa agcaatgagt
    23701 caacggaagg aaaatcttca atgaacccaa aacacgcgcc aatttttgaa ccgatcacga
    23761 tcaatggatg cgagatcaag aaccgtattg tcatgtcgcc tatgaactgc aactatacgg
    23821 cgccggatca ctacatcagc aggcagcaga tggcctatta cgccgccagg gccaaaggcg
    23881 ggaccggcct catcatcacc gaggcgcagg cggtatccac gctgccctgt gcggacacct
    23941 accgcaagta caacaatccg tacctgaccg attatcgtta tgtgcccctg caatccgagc
    24001 tggtggaaca tgtgcattcc ttcggcgcca agatcttcgc ccagctcttc accggtcccg
    24061 ggcggcaagg ttcccacgat ataggagctg cggagccggc ttccgcctca gccattccct
    24121 ggcgctggca gtttcataag gccatgaacg gctctatgaa tatgaaggtc ttgaagggag
    24181 ggatcaggat gtcgggatac catggcgcta tccccgatat cgagaaagac cccgaggcct
    24241 tcatcgccct ggtggataag atccccatga tgcatatgat ggggcggccg ccccgggaaa
    24301 tcacgcggga agagatcaag cagcttgtca tcgatgaagg caagggagcg cggctggcga
    24361 aactggccgg atatgacggg gtcgagatcc atgcctgtca cggctatctg gtgcactcct
    24421 tcctagtcca acggtccaat ttccggaagg atgaatacgg cggcagtttc gagaaccgta
    24481 tccgttttct gctggagctg gtcaggtcgg tgcggcggac tgtggggcct gattatccgg
    24541 tgggggtgcg attctccgcc tccgacgacc tgcccgacgg gtacgatccc aacgtcgccg
    24601 ccatgatagc aaaacggatg gaggaagaag gggcggactt catcgatctg tccgacggtt
    24661 cgtatgaggc catgagcgat tttctgccca acaccgaggg ccaggtcatg gacaagtccg
    24721 ccgtcattca ggctgcggtc aagatcccgg tcatgtgtcc ttccgttcac aaccccgaca
    24781 atgttgcgga tgtcctgcaa aaaggcaagg cgcagatggt tctgcagggg cgccagcaga
    24841 ttgcggatcc ggactgggtg aacaaggtga aggaaggcag agagaaggag attatcaaat
    24901 gcacccgctg caacctgggc tgcctcatcc gctttgtggc gatgcttccg gtacggtgca
    24961 tcaagaaccc gcgcacggga ctggaagagc atatcgagga atataccaga cgccccatcc
    25021 tgccattgaa gaatcgcgtg tggcagaccg ttgcagattg gggtcgcaag gaaccgtccg
    25081 agacatggga atacaccaat gaagaaatcg agcagatcat gaacgtatga ggatagaaac
    25141 tgacataggc tcataccata cgtcaacgga ttgcgataaa tataaaagga gcaccttatg
    25201 gcaaaaataa acgatgatgg tttttttaaa atcgacccgg aaccgcacct gattggtaaa
    25261 aatggttcct actatgaata tttccccggg tataagatgt ggtggaaggc gattgataat
    25321 atttcaaggc cgctgttctc atgcacccat cccacgatca tggcaaaggc ttacggtggg
    25381 ctggaagaat gggaagcact gaagaatcct gacccttcaa acatcctgca cgccatggac
    25441 aagtatggcg tggatattgc ttgccttctg cccgaatcgt gcatggatac ctgcggctat
    25501 tcgggccggt tctgcacgaa cggggaaatg gccgagatcg tggaatcgaa tcctgatcgc
    25561 ttcatgtatc aacccaatat gtctcccatc aagttcaagg gcgtcaagaa cagtataagg
    25621 gaactagagt actgggtaaa agaaaagaag gcaaagatat tcaaatatct gtgcaccgaa
    25681 gatacctaca tcaatgatcc tgatctgtgg cccttctatg agaaatgcga ggagctcggc
    25741 atcgtgctgg acattcatac cggcatgtca tggtgtccgc cgcaggcgag caaatatgcg
    25801 aaccccgtat tccttgatga tgtatgccgt gatttccctg atctgaagat catcgcgttt
    25861 catatggggt acccttattg tgatcagttg aacatggtgg ccatggggca cgcgaatctg
    25921 tatgtctgcc tgagcctcct ggttccctgg gcgatcactg ctcccagaaa atttgcagcc
    25981 atcattggtg aagccttgcg gtgggtcggg cctgacaaga tcatttgggg aacggactat
    26041 gcaggttttg ccctccagat caagaacgcg gttgagggct taagggattt ccaaataccc
    26101 gaggacatgc aggaaggcta tggctacccg cccataactg atgaggacag gcgaaagatc
    26161 tttggcggca accttggccg tctcttgggg attgatacga ctaagcgtcg ggtgaaatca
    26221 acataaggaa agaactgtcc atgcaacaag atatcggatg tgcaggacaa ttgcgggttc
    26281 gagctgctcc ttgcgccaaa aataaccgaa acactgccac tgcctgaaat ctaattggaa
    26341 atgcttcgaa aaaaagtcga tccgcaccgg tacgttagcg ggagataaaa agaagattgt
    26401 caaaatagaa agtcgatata ttgatgaata gaggtgtcgc atgggcatat cacgattgaa
    26461 atatttttta attattaaat tgacatttac aaaccttcca tttactttcc cttaatgcta
    26521 atacaatagc aaaccatatt tataatatgt taataatatg agcacaccac gaaaaattaa
    26581 aacaccttta actggtgaag ttatcacttc tcttcatgcc ggtgatatgg taatgttaag
    26641 tggcgaagtt tataccgcgc gcgatgtggc gcacaggcgt ttgtatgaat ctctgctgaa
    26701 aggcgaaaag ctgcccatag atttgtcgca agccgttttg ttttacgcgg cgccaacccc
    26761 tgctccaccg aaaaaaatca tcggctccat cggtcccacc accagctacc ggatggattt
    26821 cttcacaccg aaattaatcg aaaacggcct tcggggaatg atcggcaagg gcaaccgttc
    26881 gccggaagtt gtcgaagcca tcaaaaagta caaatctgtt tatttcggag cgatcggcgg
    26941 gattgccgct ttaatgtctc aatgcgtcaa aaaagtcgaa atggttgcct atgaagacct
    27001 cggtcccgag gcgatcagaa aactgacagt cagagatttt ccgctggttg ttgtcaacga
    27061 ttcctcgggc aaagacctgt atgtttcggc aaagcaaaaa tggcatatct ctttaatata
    27121 actagctatt tcagcgttcg tgaaaatctg ttgactgaaa tttgcttcta tattatgaat
    27181 gatggtcatt tttgtatcac gataaggaga cgaaaataaa tgatagaggt tagatggcac
    27241 ggacgtggcg gtcagggagc ggtgacctcg gtggagttgc tggctcaggc ggcaattgca
    27301 gccggaaaat acgctcaggg atttcccagt ttcggtccgg aaagaagagg ggcgcctgta
    27361 gccgcattta cccgtgttgc cgataaaaaa atcaatgtcc gctcaggcat ctatgagcct
    27421 gatgtggtgc tggtgctgga ttccagtttg atcggtctgg tgaatgttct cgatggtttg
    27481 aaaccgggcg gcaaattgat tgtcaatacg cataaaactc ctgaagaaat tcgcaaggcg
    27541 cttgattaca aggggatatt ggccattgtt gacggcaccg gaatagcacg taaggaaatg
    27601 ggcgtgccta ttgccaacac caccatgatc ggcgctcttt tgaaagtggc cggcattatg
    27661 gaactggatg cgatgaaaga atctatagag caccgtttcg gcagaatcgc gcagaagaat
    27721 ctcaacgcga tgaagcgggc ttacgacgaa ataacaatat acaattaagg ggtagatctc
    27781 aatggcattg aaaacatgga aggaacttcc cattggctgt gttgtgtccg aacccggaag
    27841 cgccagtgaa tatcatacgg gaagctggcg gtctcaaaga cccatctggg ataatgcaaa
    27901 atgcatcaag tgcggcattt gttatgttta ttgtccggaa ggttgcgtgc agcagaccga
    27961 ggacggcttc tttcaggccg atctggatta ttgcaaaggt tgcggtattt gtgcgcatga
    28021 gtgttggccc cgcgcgataa agatgttgga ggaggagtaa tatgggaaaa cgcattggaa
    28081 tggaagtggc aattgccgtt gcggaagccg tgggtctttg cgatattgac atggccgccg
    28141 tttatcctat tacgccccag tcgcacatcg cggaacatct ttccgatctg gttgccaacg
    28201 gtgaaattga cgcggaattt gtcaccgttg aatccgagca ttcggcgatg agcgcggtga
    28261 tcggcgcctc cggcgcaggt gcccgtgcgt ttacggcaac aagttcccag gggttgatgc
    28321 tgatgcacga acttcttccc attgcgtcgg cgatgcgcct gccgatttgc atggcaattg
    28381 ccaaccgcgc ggtgtccggt cccctgaaca tattaaatga tcacaccgat atcatgcccc
    28441 agcgcgattc gggctggatt tctctttttg tggaaaacgg ccaggaagcg attgatatgt
    28501 cgatcattgc tttcaaaatt gccgagcaca aggatgtcat gctgccggtc aatgttaata
    28561 tcgacggttt ccagttgacg cacatggtgg agccttttga aatgccctca aaggaagaag
    28621 tggatagatt tctgccgccg tttgtcccgc acgcaacgct gcatcccgca aaaccggtta
    28681 cgatgggcgc ttttgccatg tcggattatt ttacggaaat tatgaaggcc aaggatgagg
    28741 cgctgaaaaa ttcaaagaaa gtcattgcaa atgtattgga cgaatggggc aaaatgtttg
    28801 gccgcaatta caaactggtg gaaacgtata aatccgaaga tgccgaagtc attatgctga
    28861 ccatgggctc gatgggtgaa accgcacaga tggctattga tgaccttcgt gccaaaggcg
    28921 tgaaggccgg tctggtgaaa ttaagattgt ggcgtccctt tccgtttgag gaaatcaaag
    28981 ccgcgattaa aaacgcgaaa aaactgattg tgacggatcg tgccatatcg tttggcggcc
    29041 ccggcggtcc ggttttttcg gaaatcaaat ccgccatgta tgcggcaact cagcggcctc
    29101 tgatatacaa ttatatttac ggactgggtg gtcgcgacgt gacggtgggc gactttatcg
    29161 gcatgtttga aaaagttctg gcggatacgg aacataaagc agtggatacc tatgaatttt
    29221 ggggagtgag agaataatga atatcgtaga aaattttgat ttatttgccc cgcggctgat
    29281 taataaaaaa gaactgatct cttccggtca tcgcgcctgt tcgggttgtg ctgaagttct
    29341 ggctgtgaga ttgatgtgta aggcgcttgg tgaaaatacg gttatcgctt cggcgaccgg
    29401 ctgtatggaa atcatctcca gcatgtttcc cacaactgcc tggcgcgtgc cttggattca
    29461 tgtggctttt gaaaacacgg cagccgttgc atccggtata gaagccggac tcaaggcatt
    29521 gcgcagaaaa ggaaagatcg acgaccgtta cgtcaaggtg gtcggcatgg cgggcgacgg
    29581 cgggaccatg gatatcggga ttcaggccct ttccggagcg atggaacgcg gtcatgatat
    29641 gatgttcgtc tgtttcgaca atgaagcgta catgaacacg ggtattcagc gttccagcgg
    29701 tacgccgatg ggcgcttcga cgaccacggc gccggccggc aaagtgagtt ccggacagaa
    29761 aacatggaag aagaacatgg cggaaatcat ggtcgcccac gagataccgt atgtcgcaac
    29821 cgtttgtccg agttacccgc tggattttat ccgcaagatt gaaaaggcga aaagtatcaa
    29881 gggaccgtcc tatatccatt gtttctctat gtgtccgacc ggctggcggt ctccttcgca
    29941 tacggcaata tccatggggc ggctggcggt ggaaaccggc gtatttcctt tgtttgaagt
    30001 ggagcatggc cattaccggg tttctccgga tatgcccaaa aagctcaggc ccatccggga
    30061 gtacttcaag ccgcagggga gattccgtca tttgaaagat aatgaaattg atcagattca
    30121 ggaacgcgtt tccatggaat atcaaaaact gatggaaaaa gccaagtatc tgaaaacagg
    30181 caattaagag gaatgtcctc aaaagaatac cgaggtgaaa aaagcatgac aaaagctagc
    30241 agcatcgcag caaaaaccaa agagattgtt aaaaaacacg gtaaagataa aagtgcactg
    30301 attgccgtct tgcaggatat tcaggagtcc tttaattacc tccccaagga tgctctgaaa
    30361 acggccggca aagctctggg cgttcccttc agccgcgttt acgaagttgc cacgttttat
    30421 acggccttca gtctgaaacc gcgcggcgag cacatcgtga aaatctgcat ggggaccgcc
    30481 tgtcatgtgc gcggcgcggc ggccattcag gataaaatgg agcgaacatt atgtatcaaa
    30541 ccgggcgaga caacaccgga caataaattc tctctggaaa ccgtcaactg cgtgggcgcc
    30601 tgcgcgttgg ggcctgtcgt agttatcgat accgaatatc acggccagat gaccatgaat
    30661 aaagtggata aggttatcgg caagttcaaa ggagaggggg agtaatcatg ataaaaatac
    30721 aaaacgcaca ggtcttgcac cagattcagg aagatctgaa acgaagttac gaccccaatc
    30781 agaaaataat caccatttgt gccgggaccg gatgccgtgg ttatggttgc atcaaggtca
    30841 aggatgcact ggaggcggaa ctttccaaac ataatttaaa cgtggaaatt cgagcgactg
    30901 gctgtttcgg tttttgtgaa aaaggtcctc tggttgtcat ttacccggaa aaaatatttt
    30961 atcaacaggt caaggtgaat gatgtaaaag atattgttga aaagaccgtg gcaaagggtg
    31021 aaattttaga aaaactcctg taccgcgatc caaatacaaa ggagacggtg ctcaaggaag
    31081 aagatgttcc cttctacaaa aagcagttgc gcctggtttt cggatccaac ggcattatcg
    31141 atcctacccg aatcgaatcc tatatcacca ttggtggtta cagtgcgctg gccaaagcct
    31201 taacggaaat gaatcccgaa ggcatcatca atgaaatcaa aaaagcatcg cttcgcggac
    31261 gcggcggcgg cggatttcag gccggaacga aatgggaggg ttgccgcaag gctcacggct
    31321 cgcccaaata tatcattgcc aacggcgacg aaggagatcc cggcgcgtat atggatcgct
    31381 cgctgatgga aggcaatccg cacagtatcg ttgagggcat gatcatcggc gcttacgcga
    31441 taggcgcttc tcacgggtat atttatgtgc gcaacgaata tccgctggcg gtcattcatt
    31501 taaccatggc catcgaaagc gcccgggaag ccggactgct ggggaaaaat attctgggca
    31561 gcggatttga tttcgatatt tcgattaata agggcggcgg tgcttttgtc tgcggcgaat
    31621 cgtccgcgct tttcgcgtcc attgaaggac gcgccggcga accgcgcgcc aaatatgtcc
    31681 acgccgtgga aaaaggcctg tgggacaaac ccaccgttct caataatgtg gaaacctggg
    31741 ccaacgttcc tttgattatc aataacggcg ccgactggta cgcttccatc ggcacagaaa
    31801 agagcaaggg caccaaaatc ttctcgctgg tgggcaagat caataacacg ggactcgtgg
    31861 aagttccgat gggcatgacg ctgcgcgaga tcatttatga cattggcggc ggtattccca
    31921 acggcaggaa gttcaaagcc gttcagacgg gcggaccttc aggtggctgc attcccgaaa
    31981 gcctgctgga tttgccggtg gatttcgata agctctatga agtcggttcc atgatgggat
    32041 ccggcggcat gatcgtcatg gatgaccgtt cctgcatggt ggacgtggcc aaatattttc
    32101 tctccttttt gcaggaagaa tcctgcggca agtgtgttcc ctgccgtgaa ggtatccgcc
    32161 gcatgcggga aattctcgaa gacatctgcg cgggcaaggg tcaagaaggc gacatcgagc
    32221 ttctggaaaa aatagcaacc ggcgtggcgg acggttcttt gtgcgcgctg ggtagctcgg
    32281 cgcccaatcc ggtgctcagc acgcttcgat atttccgcga tgagtacgac gcgcacatca
    32341 aagaacaccg ctgtccggca ggtgtctgca aagcgctgat tacctacagc atcgatgccg
    32401 ggaaatgtac cggttgcacc ctttgcagca aggtatgtcc cgttcaggcg atcagcggcg
    32461 aaaagaaaaa agctcacacc attgacaatg acaaatgcac ccgctgcggc gcgtgctttg
    32521 aaagctgcaa gtttgacgca atcaacgtga aatagaggta ggataaatca tggtaaattt
    32581 gactgttgac ggaaaaaata tatcagtgcc ggaaggaacg atgcttctgg aagccattcg
    32641 cggtgcaggt atcgccgttc ccacgctttg cgcacatgag gcggtttccc gttccggagc
    32701 ctgccggctt tgtgtggtgg aaatcaaaaa gggaaatcgg accagaattg taacatcctg
    32761 tctttatggc gcggaagaag gtttgattgt ggataccaaa agcgaccggg tgctgaatgt
    32821 ccgccgtctg gtcatggaac tgctgctggc ccgctgcccc gaatcggagg ttttgcaaaa
    32881 actggccaaa gaactttgcg tggaacccca ggcaagattt attccggata cggataaagg
    32941 caaatgcatc ctctgccgct cctgcgtgcg cgtttgtgaa gaagtcgtcg gcgtgaacgc
    33001 catcggtttg ttctcccgcg ggtcgcacaa aaacgtcgga acgccttaca gcgaaaaatc
    33061 ggacgcctgc atcggctgcg gcgcttgtgc ttatgtctgt cccaccggtc atattgaaat
    33121 gacctcgacc ggcgacaagc gcaaaatctg gggacgcacc ttcaaaatgc aggcctgttc
    33181 aaaatgcggg aaatattttg ctccggttga ccagttgaaa ttcatcagca aaaaaaccgg
    33241 tacgccgctg gctgaactga caatctgtac cgaatgccgt tgattgcgac aaacctcttt
    33301 tgagtcaatc cagtctaaag gcccggtttt tttaagccgg gcttttttgt gccgtgttgg
    33361 cggcattttg atcaaaccgg tccatccttg aactcgccgg tttacttatg ataaacaaag
    33421 cacatagaaa aaaattatct ttgtcgccag gatgcctgac atggccaaaa tcgtattgtc
    33481 gctggttcct ttatctggcg agatcgtgaa acttcttatc cggcaaacac ctgatgtgcc
    33541 tgatttcgag gtgattgccg gttatgagat gtctgtcgac gaaatcaaaa taaatctttc
    33601 cagggcggat attgttctgg gcgattacac cttccaacat aagataacgg ccgatatcgt
    33661 agcggaggca aaatctctca aatttattca gcaacccagt gtgggctatc agcacatcga
    33721 tgtggaggcc tgcactgccc ggagaatcaa ggtcgccaac acggccggcg ccaatgccgt
    33781 cagtgttgcg gagcacgcca tcgcctgggg tctttgtctg attaaaaaca tgttttacgt
    33841 ccataccagc acccggtctg gcgcggatct tcaacgccga agccgatgtt ctgcaggcaa
    33901 tccgtgataa aacgctcaag gaaggagaag tcattgtcat ccgttacgaa ggtcccagag
    33961 gccgacccgg catgcctgag acgcttgcgg tgaccatggg acttgacctg gccggtttca
    34021 cgaatgtggc gctggtaacg gacggccgct tttcaggagc cacggcggga ccctgcatcg
    34081 gacatgtttc tccggaggcg gcgagcggcg gaccgattgt cgttattgaa gatggcgaca
    34141 tgatcaacat cgacatcccc gcgcgccgtc ttgacgtgga tttgaaaccg caggaaatcg
    34201 cccggaggct ggccggttgg aagcctctgc ataagcagat tacccccggc tacatgcgcc
    34261 gttatgtccg gatggtcggc tcggcggcat atggcgctgt tcttgagtga atccgcttga
    34321 aagtattccg gtgggattca ttttttcaat cccgtcaggt ttcagacatt tgtttgaaac
    34381 ctgatttttt tcttgagggc agaagccatc ataaagtaac tgaagcacgt atgaagatgt
    34441 gataaaatta atgaaattat cattgaaagg tgctgctgtg gataaggggt acatgcgaaa
    34501 aatgagtttt aatgaacgta cgtatgtggc gacggattcg ctctgccctc cggtggccaa
    34561 ccagtttgtt tttgacgggg aaggtgtgct tgatccgtat ctctggcgca ccgctgtggc
    34621 ggttgcctct gaagccaatc ccggagcgag gctcattctc aaaggtcatc tggcctggag
    34681 ccgctggatc gattccggca atccgccgcg tgttcgggaa atagacggaa gccgatggga
    34741 cggtcatggg ccggaaggag cgcctgtttt actggaaccg ctgccgtttc gtgagagccc
    34801 gatctgcgag gtgattcttg tacatggtcc ggttccccgg gttgtttttc gcacgcatca
    34861 cggtgtcatg gacggcagag gaacgctgtg ctgggccgag gacgtttttc gggccctgcg
    34921 gaaagaaaac gtcatgggat cgacgtcgac cctgacggaa gttcagatgg ctgaaagttt
    34981 tcaaaagcag ttccgccgcg cttttcccgt cgaacacatt gcgcctacgg gacttcccga
    35041 aggcgacgcc cggggtgtta tctggaaacg tcgcagcatc aaaggcaagg tgccgctgct
    35101 tttgccgcga tgcgcccggt ttgctgcgga agaagcctgg cggcatgcgg aaggcatcgt
    35161 gcggtttgcc gtggcggtgg atatgcggcc ccgtcggccg gagttgcgca gcaccggcaa
    35221 tctgacgttg gatctttatg tcgagatcaa caaaaatacg acgccggaac tgattgccga
    35281 cgacatggcg tatcaactcc ggcacggttt cgaaggccgg ataaaaaaat atgatcaact
    35341 ggtaaaatat attccgctgc gactggtgcg gttcggggcg caaaagatga ttcgtgaccg
    35401 gcatcgcaag ggacgctacg ggctttcggg cattttttcc aatctgggcc gggtcgatct
    35461 gaattttttc tgcggaggcg gattttccgc ccggtcgttc tgggctgtcc cgccggcgac
    35521 ggagtatttt ccgttttttc tggtactgtc gggatatgaa ggcggttcgg aactggtgct
    35581 ttcggttccc aaaaacctgg gcagtgcggg aagacttgat gacactctgg aatgcatcgc
    35641 ccggaaattg gaacaagtaa cccgctaaaa gcgataaacc gtttttattt gcacaaaatg
    35701 tccctgcgct gaattttcag tataaacatc gcaaagataa gtcaaggtga taccgaaccg
    35761 ggaaaagcga taataaatat cggccccaac ggaaaggact gctctggcgc tgtcgtcgac
    35821 tgccgtcccg ttttgttttt tttctttgct ttgcaaccag ttgaccgaag ggcccagttt
    35881 gaacttttcc gtcagatggt aacccagaag gcactgcaga tgaaactcgt cgcccggcag
    35941 aacatcggtt ctttcagttt caaaacggaa gacgtatttc acagcggcat ctatgctgaa
    36001 gtcgcccatt gatttgtgaa gaaaaacaac gggttttatg tcgagctgat tcgagccgat
    36061 gttggctgat tttgcagagc tgtattggcc tgtgggatat tccacaaata tcatcgggag
    36121 aatatcaacg tttcgaatcg gcagaaaata accggcgccg agattgatat cgccaatccc
    36181 tgaagattcc tgatccagag aatcgatttt cattaaaccg aacggaacca gagccgtggc
    36241 cacaaaatca ggcgaataat agcaaagacg caacaattcc tgggcgttca gcagccccat
    36301 gtctttgctc gcgtgattgc ctttgccgtt tgttgtttta tcagcggcat aaacggaagt
    36361 gtaggtgaga aaataagttc cctgttttgc ccttgcaccg tcgtaaaaat ttacggcaga
    36421 cgccgtatca ggcagcacaa gtaacaagaa aaacaacgcg aaaatggatt gtcgccggaa
    36481 acccgataaa gccaaaattg aatcctcgaa accgtttttt ttgagaatat gcttatacag
    36541 aacatttcgc cgggggcaaa gcataaatat ttcacctgtc ggccatcctt ccatatctgt
    36601 ttaaaaaggt ttaattgtct tgacaggcac aggccgcctc cctataattc agacgttcgg
    36661 tctcgcaacg gcgtaaatta aattttaacc ctacaactga aaaagggaat aatgtatgtg
    36721 gaaggataag gttgtttctc ccagtgaagt actgtctcaa atcgagccgg gcatgaccat
    36781 ttttctgggg acgggcatgg cggaaccgcg cacgctggtt aaaaatctta tggcgtcgga
    36841 agagccgaac ctccaggatc tggagttgat tcagctggtc agtatcggcg ataccatacc
    36901 gattgatgaa cgatattccc gcaaatttcg tctgaggact tttttctccg gctggattgc
    36961 gtccgaagcc gtcagcgcgg gaagaatcga tcttatcccc agccggtttt ccaaaatccc
    37021 ccagctgttt aagtcgggcg ccattcacat tgatgcgacg ttcatccaga tatctccgcc
    37081 tgatgaaaac gggtatgcct gcctggtcgg cgtggatgtg gagcggcagg cgatggaaac
    37141 cgcgcatctg gttgtcggcg aaatcaatgc caaggcgccc cgcatcatgg gcgacacact
    37201 ggttcatatg gatgagttta atttttttgt cgaatccgca gaaccgccgt tgtatattcc
    37261 ccgctggccg gtggaagatg tttttctgaa aatcgccgcc aatattgccg cggttgttga
    37321 agacgccagt tgcgtatcat tcgggattgg ccctctttac gaagccctgg cggttcagct
    37381 cacaacgaaa aaaaatctcg gcgtccattc tccttttttt acggattctc tgatggacct
    37441 tgttcagagc ggcgccgtga ccaatcgcta caaaggcgtg ttccgcggca agtgtgccgc
    37501 gtcctatctg ctgggcagtg aaaaactgat gcgctggctg gaccgcaatc cgctggttga
    37561 atttcagccg gaggatgtga ttaccgatcc gaaaataatc ggcacgaatg acaaaaccat
    37621 atccattctg ccggccagaa aaattgacct gacaggcaat gtcgcgctgc ataccggcaa
    37681 ggggaatgtc accgccggtc cgggaaatgt ccaggaactt tttatcggag cgtcgctttc
    37741 caaaaacggc cgtaccattt tcggattgcc cagccgcaac cggaaaggtc tgcccaatat
    37801 cgttctgtcc gtcgataatc tgccgttcca gtttaccaac cgtgagtcga tggacctggt
    37861 ggcgacggaa tacggtgtgg cgtatttgat gggcaagacg atgcgcgagc gtgctcagga
    37921 actgattgat atcgcgcatc ccgacgatcg ggaggaactg gtccgtcagg cgaaggaagc
    37981 gaaactgctt tacgccgatc agatttattt ccccgagtcc ggacatctct acccggccag
    38041 attcatctgt tcgcatgaat tcaagggagg tctcaaggtt cttttccgcc cgatcaaacc
    38101 gtccgatgaa gaaaagatgc gggacttatt ctaccggttt tccgatcagg ccgtttattc
    38161 ccgctacttc acgtccatta aaacaatgcc tcataaaaag atgcaggaat acgtcaacgt
    38221 caattatcgc tgggtcatgt ccatcgtcgg cgttgtcgaa gcggcgggca cggaaaaaat
    38281 aattgccgaa gcgcgatacg cccgcacgaa acaggatgcc tttgccgacg tcgcttttgt
    38341 tgtggatgag gattatcaga atatggggat tgcctcgtat cttttcgaac tgctgatccg
    38401 tgcggcgcgc gaagagggca tcaagggatt caccgccgac gtgctggctt ccaataaatc
    38461 catgctgaaa gtttttgaaa aagcgccttt tcccgtgcag accgttttgt cccgaggtat
    38521 ttacgaacta accattcctt ttgctgatgc ttaacggatg ggcatggcag caaggccggc
    38581 gacggactat cggcgttttt tgagatagcc ctccaggcgg ttcaggcctt ctttgatgtt
    38641 ttccagggaa ttggcatacg aaaagcgtaa aaacccttcc ccgccgctgc cgaaatcgat
    38701 gccgggtgtg acgccgacct tgacctcttc ggtaatgcgg aaagcttctt tataggaatc
    38761 cttgcagaat cggcgcgcat cggcaaacac gtaaaaagca cccgtcggtt cgacgtgaat
    38821 gacgaaaccc atgtcctgca gacgggacag catataccgg cgccgttcat cgtaaatccg
    38881 caccattctg gccacgtctt tttgggattt tgtcagggcg gcgatgcccg cccattgaat
    38941 aaacccgttg gcggaaatca taaagttctg atgaatgcgc tgcatgactt tggaaaacgt
    39001 tttgggaaag atacagtagc ccagccgcca gccggtcatc gcatagagct tggagaatcc
    39061 gttgatcacg aacgctttgt ccgtgaattc caggatggaa tgcgcgcggt ctttgtaaac
    39121 caggccatga taaatttcgt cggagataat atactgtttg tcgaactgag cgacattctg
    39181 caattgttct tcggtcatga cgatgccggt cggattggaa ggcgaattga tcataatcgc
    39241 ttttgtccgt gaagagagtt tctttttgat gtctttcgga cggtattgga atccgtcttc
    39301 gggataggtt ctgacctccc gggttttgcc tccggccgcg agaataaaat tctggtaaca
    39361 ggggtaacgc ggattggaaa taatgacctc atcacacgga tcaaggatcg ccagcatcgc
    39421 gaaaagcaaa gcgggcgacg tgccggtgga aaccaggacc tgatcgggtg taatgctgac
    39481 gccgtattcc agaaaataga aatcgcagat ggcctgtctg agttccagca gtcccagcgc
    39541 gtgcgtgtac agggttttat tcttggccag tgcgtccctg gctgccttca taatcaccgg
    39601 cggcgtcggg aaatcaggct cccccacttc catatggatg actttttcgc cttgacgttc
    39661 cagttcttcg gctttcgcca taacatccat cacaatgaaa gacgtaaatt tggacgctct
    39721 tttggaaatc atagataccc ctcgtttgtg atagggtacc tctattttga ttgtataaaa
    39781 atttcaacaa cttttctttg ccgggagtgg atgctctccg cagcacgctg cgatgaattt
    39841 tcgacgggca ggacattaat atcaatttaa tgtcgattta accgtctgct gccggacgat
    39901 taaatatctt tgaaagaaaa aacggggcac ggccggacat tcaaacttgt tctctttcgc
    39961 agtgccgaag aatcttcttt acaaaataat ccaatttgtt accctaagtg ctattaaaaa
    40021 aagaaaaatg aataacagag atccttcaga tgagaacagt attaatcatc atcctgattg
    40081 ccgtcagttc tttaggaggc tgcaacatgc aaaaccgctt tctttatttt cccgatgcca
    40141 cgtggccgac aaaacaggcg ctggcaaatg aaaacatgaa cttctggcag gcgacaggag
    40201 atgattatca gggtttggtt tccgccattg atgcgccggc acccaacggc accattgtgc
    40261 tttttcacgg caacggcgga actgccctgg acagagggtt ttatttgacc ccttttatga
    40321 accttggatt ccgcgtcatt ctcgctgaat atccaaagta cggcggccgt cccggtaaag
    40381 ttggagaaaa accgtttgtc gccgccggcc tggagacggt ccgcctggcc cgcgagcact
    40441 acggagatcc tctttatctg ggcggcgaat cgctgggctg cggtgtggtt gccgcgattg
    40501 tccgacagac accggttccc gtcgccggta tcatgttgat aacaccgtgg gatacgctgg
    40561 catctgttgc caaatcgctt ttcccttttt tcccggttaa actggtattg actgatcaat
    40621 acgacagcat ggaaaatttg aaaacattta aaaataaaat tgccgtcgtt ggagcggagc
    40681 gggatgaaat tctgccggtc aaacacgcga taaatttata taacgccctt cccgaaggcc
    40741 ggaaacgact gtggatcatc aaaggtgcgg gccataacaa ttggccgatg tttaccgacg
    40801 tttccctgtt cagggaaatc gtcgattttg tgaaaattga tgtacatcaa tgaaaagaag
    40861 ttttttccac cctatgattt gtccatatca tgtaacggag aaatcttatg aaacaagaga
    40921 agaacttgaa aatcggccgc cggtcttttc tgaaactgac ggcgggagcg gcagcgcttt
    40981 ccggtatgag tctgccgaaa aaatcttttg ccgggacaga gcaacgctta tcaacgctga
    41041 ttgatctgag tctttgcgac ggctgtgtcg atcgtgaaat tcccgcctgt gtcggtgcct
    41101 gcaaagaaat caacagggat aagattccgc aggtgatcga tcccattccc gaaccctggc
    41161 cgagaaaaac cattgaagac tggtcgcaca agcgcgatgt tgccgatcgt ttgacccctt
    41221 acaattttat ttatgtacac aaggccgacg tcagggtggc cggtcaggca aaaacaatct
    41281 ttgttccgag acgctgcatg cactgcgata atccggcttg cgcgaccatc tgcccttttt
    41341 ccgcgaatca caaacataaa agcggcgcgg tggtcatcga tcagaatctc tgtttcggcg
    41401 gtgcgaagtg ccgcgatgtc tgtccgtggg aaattcccca gcggcaatcc ggcgtcggca
    41461 tttatctcaa tgttctgccc ggcctgatgg gcaacggcgt catgtataaa tgcgatctgt
    41521 gtgttgatcg tctcacgcag ggcaaactgc ccggctgtat tgaggcctgt ccgcgggaag
    41581 cgatgctgat cggttccaga gcagccattg aaaaagccgc cctgtcccgg gccgcccgca
    41641 tcaacgggta tctatacggc aaaacacaaa acggcggcac cgccacgctt tacgtttctc
    41701 cggttccttt tgaggaaatt aataaaacaa tgataaagaa gccggggcag ccggacatga
    41761 aaatgaacgt cgagcgtaga atggtgggaa cggatgctct ggggaaggcg gttctggctg
    41821 cgcctgttct gggcctggcg gcggctgctg ttgttggagt gtggggacgg atcatcagcc
    41881 gtaaagaaaa agcgggcagg gaggaatgag accatgaccg ataaaattct gacacaagaa
    41941 aatcgtgttg tccgccacgg cgcgatggaa ttgatcgagc actgggccat cgccctctcg
    42001 ggccttatcc tgcttttaac cggaatattt gagctgcccg tcggcaaacg ctactatatt
    42061 attgacgttc cgggtctggg ctggtcggcg gactttataa cgtcactgta cctgcattac
    42121 gcggcggcgc tggtgtttac ggcagcggcc gtgttccatg ttttttatca cggtctgcgg
    42181 ggcgaaaaag aactgcttcc gcggaaaggc gatttgtccg aatcggtaac cgtcatcaag
    42241 agtctgtttg gattcggtga agagccgccc atgcgcaaat atctgccgga gcagcgtctg
    42301 gcctacgccg caatggcctt gattattctg atgctgatca tatcggggat ggtgaagaca
    42361 tacaaaaata tttacgcgcc ggatatgtcg catggcattt tgctctgggc tacatggatc
    42421 cacaatatcg gttttatatt gttcttcctc gcgtttctgg cccacatggc cgccattgcg
    42481 ctgcggccca actggccgat ggtgcgcggg atctttaccg gcagggtgcg tctggattac
    42541 gcgaggcatc gtcatccgct ctggattgcc ggaatggaac ccgaaccaca ggatccggcg
    42601 acggttgagg aaggcgaaca acagccgctg ccatccgaag aagcgccggt gatggaagaa
    42661 acggaagaag aggatctgcc tccgggaaaa ccgcagccgg aagaagacaa aacataaaac
    42721 taattcctcc gtcttcctgt cggccgcccg gttttttttg tggtggaaaa cacgccaaag
    42781 acaggatata agcgtcatac cgtcattgag gaggaaatac ccatttaccg ccgtatcagt
    42841 ctgtttcggg tgctgcacat cagcgccctg atgatcttta tcctgctggc gctcagcctt
    42901 gtcgctgttt ctgtgggaag tgtttccatc gaaccgggaa gggttttaac gttttttttc
    42961 ggacctttga cggggcaatc gcctttatct tcagaagaag aattgattat ctgttcggtg
    43021 cgtctgccgc ggatcgtttt tgcagggatt gtcggcgcgg cgctttcgct gggcggcgtt
    43081 gtttttcagg cgatcttgcg caatcccctg gccgatccct atatcctggg catctccgga
    43141 ggggcagcgc ttggcgcgat tatcggcatt gtgatcgggg ccggtgcttt ttatctgggg
    43201 ataccgctgc tggctttcgc gggcgcgctg gcgacagttt ttattgtatt catgatggcc
    43261 ggaaaaacca gaggccccct gctggataat tcgcttttgc tgtccggtgt cgttgtcaac
    43321 gccttttttt cggccgccat tctttttttt ctatccattg tcaacagtat ggaactgcac
    43381 agcatcacgt tctggctgat gggcgatctt tctcacgctt cagccaggga aattcttgtc
    43441 gccgccctct gcctgctgtc cggttttgtt ttgctttacg cgcaggcgcg aaagttgaat
    43501 ctgattgtgc agggggagga aacggccgaa catctgggtg tgcatgtggc aagaacaaaa
    43561 aaatggctga tggtgattac ctcgctcatg attgccgtag cggtttccct ggccggcatc
    43621 atcggttttg tgggaatgat ggtgccgcat ctgatgcgtc ttgttttcgg ttccgatcac
    43681 cgtttgattc tgccggtctg cgcgttattc ggcgcctcgt ttttgattgc ggccgatacc
    43741 gtcgcgaggg tgctcttggc gcctgcggaa ttgccggtcg gcgttgtcac ggcgctctgc
    43801 ggcgcaccgt attttatttt tcttctgaaa agaaagaggc tttgatcatg tctgttatta
    43861 cagccgaaaa tatccgtttc agttatccgg ggaaaccggt gatgaacggc gtttcgtttt
    43921 ccattgaagc ggcggaaatg gttgcggtga tcggccccaa cggttccggc aaaacaacgc
    43981 ttttgaaaat gatcaacggt acgctgtttc ccgatggggg gcggatgctg atcgacggca
    44041 gggaaacgac gggctggccg cgcaaggaga tggctcgcca agtggccatt gtgcctcagg
    44101 aaacggcggt gacgttcccg ttttacgcgg aagaaattgt tctcatggga aggtttccgc
    44161 atttgaacaa ttaccgtttt gaagataaga aggattaccg gattgtccgg gaggccatgg
    44221 aaaaaaccga taccatttct tttgccgccc ggaggttgga tgaactcagc gcgggagaac
    44281 gtcagcgcgt tctgatcgca agagctctgg cccaggagcc gaaaattctt ctgctggatg
    44341 agagcacggt ttttttggac ctgaagcatc aggcggagtt tttatccctg ttgaggcagt
    44401 tgaatgccgt gcagaatctc accgtgatgt ttgtcacgca cgacattaat ctggccgcgc
    44461 aacacgccca taagataatt ttgcttgatt gcgggaaaat ttatgctata ggaatgcccg
    44521 cggaagtcat taccgcggcg aacatcagag aggtttatga cgtcgacgtt ttcgtcgacc
    44581 gaaatccgca tgacggatcg cctcgcgcaa cactattgac aaattgaata ttgctggttt
    44641 gatttcaaag ggcaaccggc cggtgatccc gcataaacgg gagatccgcc gccgtctgct
    44701 tcagaaaaca aatcaggaaa ggctgccaga taaaggaagc cggttaaagt ccggcgctgc
    44761 cccgcaactg taagcggaac gaaattcaca ttgatccacg gacgttttag cgtccggaag
    44821 gagtgaaaag taggtcgccg caagccagga aactttacgg tgagcacaac ttactttgat
    44881 cccgaggggg aaaggagaag ctccatgaag aagattcttt tttttgttct gtttttttcc
    44941 gtagttgttt tttcacccgc aaacgcttcc cagaaggatg ccgacaatgc gctggtcacg
    45001 atgggcgaaa tcgttgtcac tgcaacacgc gatgcgcagg aaattagaaa gacaccgtcg
    45061 agcgtcagcg taattaccga agaggagatc aacgattccg gtgcgacgac cctggtggaa
    45121 gttctggaca gaatcgagag tattgcgttt aggacgtaca gcgggaattc gtcgcagtcg
    45181 cagatcgaca tgcgcggatt cggcggagat aatcctttcg gcaaaacact gattctgctg
    45241 gacgggcgcc ggttgaaccg gaccgacatg gcctccatca actggttgca gatgcccgtc
    45301 aacggcgtcg agaaaatcga aattgtccgc ggaccgggaa gcgttctgta cggcgatgcg
    45361 gctgtcggcg gcgtgatcaa tatcatcact aaaaaaggaa aagggaaacc gaccgtcaac
    45421 ctgtcggcca tcggcgggag ttacggtctg cacgatgaac ggatcggtgt gagcggcgct
    45481 accggcaaat ggacttacgc tctgacgggc gaaaacaatt tcagctccgg atacagggac
    45541 cgctcaaagt attcggcgcg cggcggaggg ctggatgtgg gatactttgc cggggatatc
    45601 ctgaacatgt cgctgggcgt ttcttttaac cgggtggatt atcagatgcc cggcgctctg
    45661 accaaagccc agatggtgaa ggacaggagg cagtatcagc ccgccatgcc ggattattac
    45721 atgaatgcca acgatgataa cgacggatcc gataagtaca ccaatatcaa gtttgatgtg
    45781 aaatcgttct ggggggcctg gggaagaacc gaagtcaatt ttctctatgg gaaaaaagat
    45841 cttcagatga acatgccgtc atggttttca tataattatt ccgacaccga tgccgagaca
    45901 ttcggcatga cgccccggta tattttggag aagaatattt tcggttttca caacaaagtg
    45961 atcatggggc tggattatta tcatgaaccg tacgcgaagg atattttcag caatcgcaga
    46021 aggacggacc ggttaagcac cgccgacttt acgcgggatg ccctgggata ttacatccgg
    46081 gacgaattca gcgtgttgaa aaacctgatt ctcagcgccg gttaccgttt cgatcgcgtg
    46141 tcggtcgaag ggtccaatga agatttttcg acgccgtcca ataattttac aaatcagaag
    46201 aatatttata aagccgaagc gtatgaggct ggacttacgt ggctctgggg cgatcagtcg
    46261 aaaatattcg ccaagtacgc caccgtttac cgaattcctt ttctggatga agtggcgtca
    46321 ttcagcggtt tcggagggaa tttcctgaag agtctggaaa aagaaaaagg gaaaagcatg
    46381 gaaatgggcg cagagtatta tctgctgaat aatctgaaac tgggggttgc tctgttccgt
    46441 ttggacatgg aggacgaaat cgaatatgtt tacgatcctg aaacgtttac gggagaaaac
    46501 cgtaataccg gtaagacacg gcatgacggc gtggaagcgt cctttgccta tctcttgcag
    46561 aagtatttca ggatatttgg aaattacacg tatcacaagg cgacctttga aaagggcctc
    46621 tacaacaaaa aagaaatgcc gatggttccc cggcacgcgg ccaatgtcgg tctggaaata
    46681 tacctgcccg gtcatgttac gctcagaacg gaagttcaac atgtgggcaa agcgtatctt
    46741 tccggcgaca gcgacaattc tactgaaaag ttggaagaca ggacattgct gaatattgtt
    46801 ctccagtaca aaccggtctg ggatacaatg aaattgaccg cgtttctggg cgtggataat
    46861 gtcgccgatg tgaagtattc gtctttcggt atcgattatg aacagtacgc catgcccaat
    46921 ttttattatc cgatgcccgg cgtcacattc aaaggaggcg tttcggttga gttttaagca
    46981 tctgtctttc aaagacaatg ccgtgatcgt attttccgtt ttgatattgg cggtattttg
    47041 cgcgggcttg gcatgcgcgc ggacggtcac cgatgaaatg ggccgcatcg tccatgtccg
    47101 tcgcgtgccg cagagaatcg tgtcccttgc ccccggtatt acggaaaccc tttatgcgtt
    47161 gggtctggag gacagaatcg tcggcgtaac cacgttctgc gactggccgc caaaagcccg
    47221 cctcaaagga aaagtgggcg ggtttaccaa tccgtccata gaaaaaattg tttctttgag
    47281 accggacctg atcattgcca ctgccgacgg gaacaaaaaa gataccgtag aaaaactgga
    47341 acgtctcgga ttgccggtgt atgttacaaa accgtcggat acccggggcg ttctggaaag
    47401 catcatgcgg atcggggaaa taaccggtcg caccaaggaa gccgacgaac tggcagacgg
    47461 gctgagaaag cgaatgaata gaataaccgc ccggatacgc ggtaaaaaca ggccgcgcgt
    47521 tttttttcaa ctggggatgg acccgatgat aacggccggc cggaatacgt taattcacga
    47581 agtgattgag aaagctgggg gcatcaacat tgccggacat gatacggcca attatccccg
    47641 ttacagcacg gaagggatca tggccgcatc gccggacatt attgtttttg cgccgatggt
    47701 caatgatcag gaattttcga ttgttaaaaa attctggggc agattcggga aaataccggc
    47761 cgtgcaaaac aacagaatct atctgataga cgccgacctg atcaacaggg cttcgccgcg
    47821 catttttgat gccgtcgaac gcatagcgtt gatttttcat cctgatataa aatgttttca
    47881 ataatgaatt tctgttgtac gatccgccac ggcctttgat ccggaatgat cgttgaagta
    47941 tttccgggga acatggagcc gacggtttgt tgaatacaat aaacggaaaa gatcaaaaca
    48001 gtgatgattg acggcaaact gccaaccaga ggcctggtct atgcggcgct tttcggagcc
    48061 atgacggcag cgggggcgtt gattgttatt cctcttccgc ccgtgccggt tactgcgcag
    48121 acatttttta tgaatgtcgc cgcccttctt cttggcggtc cgttggcggt tgtcagccag
    48181 ataatttatg tcttgctggg cgtggtggga atgccggttt ttgccggggg caaagccggg
    48241 ctgggcattt tgttcgggcc aaccggcggt tatctattcg gttttatcat tgccgctttt
    48301 gtcatcggcc atgtccatcg catgaaaaaa aacgccggcg cggtctggca ggtattttcc
    48361 atggtgctgg gcatgctggt catttatgtt gcgggaagca ttcaattggc atggattgcg
    48421 aacatgagtc ttccgcaggt gctgatcgtc ggggtagtac cttttatccc gggcgatctt
    48481 atcaaaatag cgctggctgt cctgatcagt tcccggttaa aaaaccgcat agagatttga
    48541 ggaaacgacg ttgattgttc tggaagatgt ttattgccgg tatgaaccgg aaggcattga
    48601 agccctacgc ggcgtttctc tgcaaatccg ggagggagaa cgtctggctt taatcggtcc
    48661 gaacggctgc ggcaaaacca cattgatcag gcattgcaat gcgctgcttg tgcccgaaaa
    48721 aggtcatgtc accgtcggcg gattaaggac ggatcataaa gagaaccaac ccgatatccg
    48781 gtcacgggtg ggcatggttt tccagaatcc cgatcatcaa atcgtcggga tgacggtcga
    48841 agacgatgtg gcattcggtc cggaaaattt gcgtctgcca gcggcgaaga tcagggcaag
    48901 gattgccgaa tccctcagcc gggtgggcat ttacggactt gccgcccgca atatcgaaga
    48961 tctttccggc ggcgaaaaaa gactggtggc gattgccggc gtgctggcta tgcggccgcg
    49021 ttatattgct ctggatgagc cgaccgcctt tctggatccg gcagcctccg gaagggtgct
    49081 ggcgattatc gacaaactgc atgccgaagg cgtgggaatc attcacatca cccataacat
    49141 ggcggaagct tcccgcgcgc agaaaatcgt cgtgatggat cagggccaaa tctgcatgca
    49201 gggcgatccg gaggaaattt tcagccggtt tgaaatcctg caaaaactgg ggcttcagct
    49261 tccccagatt acggaacttc tcatccggct gaagcaaacg ggctttccgg tccgaacggg
    49321 cattgtccat atggaagacg ccttaaagga aattatttcc atgaccgcgc gtcgtaagga
    49381 ttgaacatgt accgcaccgg ttcgtttatc aggggagagt cgttcattta ccggctggat
    49441 ccccgcctga aactcgctgt aaccgtcttg ttgagtctgt tgatttttca ggtcgaacaa
    49501 ccgctggttt ttgcttcagg catcggtttg tttttgctgg ctatggccag cggcatacgt
    49561 ctgcggacaa tcggtgagtc gatcagaccg gtccttttct ttatcgtcct gatttttctg
    49621 tcgcatcttt tattcagcga cgataaacgc tccatgatga cgatcccttt tttggggatt
    49681 gccttttcgt ctgccggatt aaaacaggga acccttgtgg tcggacaatt tttatgtctc
    49741 attgtcgggg ccgttttgtt gacgatgacc acggcacctt cccagatcat tgcggcactg
    49801 agatattttt taaaaccttt caaaaaactg cggctgccgg tggatgacgt tgccgtgatg
    49861 gtcgcgctgg ctttaaggct catgcccctg ttgctgatgg aaaaagaacg aatcgatgcc
    49921 gcccggaaag ccagagggtt ttgtctgcgg cgacagagtg ttgccggccg gatgaattcc
    49981 tttttgtctt tgatttcaaa aatgctgctg ggcattttcc ggaaagcgga cgatatcgcc
    50041 ctggccatgg aagcccgaag ctaccggcgt ggcgggcgtt cgtcctttgt ggaattgaaa
    50101 ttttcgtccg ccgattatgt tgtccttgtt tttttggcaa tttttctggt tatttttgtg
    50161 gcattaaatt cgcatttcgg ttaaattacc cgccatgaat gcgaaatggt ttttggtcga
    50221 tacacaaaac aaactgaagc gcgccgttgc tgatttcgag cgggcaaaaa ttctcagtat
    50281 cgatacggaa tacgattcgt ttcattattt cagggaaaaa ctctgtctga ttcagatcca
    50341 tgccaatcac acgacctacc tctttgatcc gttgggcgat gtggctttgt cttttttagg
    50401 cgattttttt gataatcagc gtattgtcaa gattcttcat gctgcggata acgatatccg
    50461 tcttctccgg agagattacg gattcggttt cagaaatatt tttgatacac accgggcggc
    50521 gtttcttctg ggtttccggg agctgtctct ggacaaaatg attaaagaat ttgtcggcgt
    50581 ggaattgaaa aaaaacaaaa aaatccagcg ttcccattgg gataatcgtc cgctgacgga
    50641 ggaacaggtt gaatacgccg tccaggatgt gatttacctt cccgatcttt atcaaaagca
    50701 gacggcggaa ctgaccgcca aaaatcttca ggaatccgcg cgggaagcgt ttgccagaat
    50761 tgccgagacg gactggcggg agaaaaaatt caaccggtgg gggtatgcaa aaataaaagg
    50821 ttataatgcc ctcaatcagg agcagaagga tctgctgaag aagttgtacc tatggcgttt
    50881 tcaccgggcg aaggaagaaa atcgcgcggt cttcatgttt ctgccggata aaaatctttt
    50941 ggatctcgcg ctggatgcgg atcatcccga aaaatattta tcacaaagga agatgcgaat
    51001 ttacggttcg gaaatcgaaa cgattataaa aaggatgtaa tcatttgaaa ttcgatcctg
    51061 aggatgtttc cagcggcaaa aaagccttca aatgccgatc tgtctttcgt agaggtggcc
    51121 cgaaaataac cggattatca agttgttgtc gaaagagatt tgataacggt ctttcgtgtt
    51181 ttcaaagcgg ggggggggaa gcgtgacggg attcatttgc atttaacttt aaagcccacc
    51241 ggtttattgt ttttaaaata gcctgtaaac gtcttgccgg tgctgcaggt ataggttccc
    51301 cggccttcga ttttgccgtt tacaaagtcg ccttcatatt tgttgccgct cggagaggta
    51361 atcttgccct tgccgtgtaa tttgccgttg ataatctgcc cctcgtattt attgccgttg
    51421 gcatcggtat acgacccatg tcttggctgg gccttttgac ggctgtccgg aacgtaatac
    51481 acattgccgc tgcagttgat cgtaaaaccc accggtttgt tattttcaaa attgccggta
    51541 aactcgcggc cgtttttgca ggtgtatttc cccttgccgc tcatttttcc ttcaaaccag
    51601 tcgccctcgt atttgttgcc gtttgtccag ttgtaagttc cccggccatg cggtttgccg
    51661 tttgccattg ttccttcata acgggtaccg tcggcaaagg tgtaacgctc tgtaataccg
    51721 gaactttcag aaaccgattt gacttctttc aatgtccagc cccgtgtttc catgcagcgg
    51781 ttatacatat ccacattggt cgacgcgcta cccgtcgcct gggctttttt catacagtca
    51841 tccatgtctt ttgcaaaatc ctgctgtgcg gcattcggct ttgaccagac tgccctggtg
    51901 gccggcgtcg tggcgcatgc tccgatgaaa acgatcatca gaaacatgac gagcatcatc
    51961 agcgatgccg ttttttcttg agtcatggca agctttttca tgtttccccc gaagattggg
    52021 tttgctgaag aattaacatt gttcagcaag acgatgataa ctgctgaatg agattctggt
    52081 ggagatgagg gggatcgaac ccctggcctc gacattgcga acgtcgcgct c
//
LOCUS       DAOSME010000010        49485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_910_length_49485_cov_19.360995, whole genome shotgun sequence.
ACCESSION   DAOSME010000010 DAOSME010000000
VERSION     DAOSME010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 49485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49485
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_910_length_49485_cov_19.360995"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..138
                     /locus_tag="PLG98_02615"
     tRNA            63..138
                     /locus_tag="PLG98_02615"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Cys,seq:gca)
     gene            362..847
                     /locus_tag="PLG98_02620"
     CDS             362..847
                     /locus_tag="PLG98_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOE32173.1"
                     /translation="MFKVGDLAVYPAHGVGVIEAIENKEVMGKKQSFYVMKIMGNGMK
                     IMIPTMSVKAVGLREIIMEKEIPKVYEILRNKDVTIDKQTWNKRYREYLERIKTGSVF
                     EIAGVLRDLFILKSDKNLSFGERKMMDTAKNLLVKEISVATNAQESEVEHNLQKIFTV
                     Q"
     gene            1010..2011
                     /locus_tag="PLG98_02625"
     CDS             1010..2011
                     /locus_tag="PLG98_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE32174.1"
                     /translation="MKRLIIRIILIMACSISGYAIAFYTSSDSFLILKSIAGFIIGLT
                     IALTVIKIEQHIRQLSLKIIIGGVAGMIVGLLIALIFSFGLNFVSKIRENQQALPWIY
                     LLITGIFSYLGLTLGSKKIEEFSFGGIDSKTAHDHRILDTSVIIEGRIADICDSGFIE
                     GELIVPRFVLNELQQVADSSDSMKRLRGRRGLDVLNHLQKSANVNIEIVDQDFPKIKG
                     VDGKLVALAKQLNAKLVTNDYNLNKVAELQGIRVLNINELANAMKPVVLPGEQMTVKI
                     VREGKEAGQGVAYLDDGTMIIVDNAQKMLNMNVRVVVTSVLQTTAGRMIFSELKEVA"
     gene            2107..3321
                     /locus_tag="PLG98_02630"
     CDS             2107..3321
                     /locus_tag="PLG98_02630"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HOE32175.1"
                     /translation="MKNNGEQSMKTVLIIPAGGAGQRFKSHVAKQYLLLDALPVLVHT
                     LKVFQDSPVVDEIILVLPQDDVVHVRQDLIEKYGMSKVVAVVAGGRERQDSVKNGLDA
                     IQGNAGLIIVHDAVRPFVTDQMIGRVVAAARACGAAVTGVQVKDTVKATKDDHMIQST
                     VPRENLWLTQTPQAFRFDILKDAYRRAYEENFYGTDDAALVERTGKEVKMVEGSYDNI
                     KITTREDMLVAEAFMKKRNTAGIRSGFGYDSHRFTEGRKLVLGGVEIPCDRGLEGHSD
                     ADALIHAICDALLGAAGAGDIGRHFPDTDPQYKDISSMILLKNVKKILDGKGFRIQNI
                     DTTVILEVPRLAPYADAIVANIASVLNISEECVNIKAKTNEGMGFTGRREGVAVFAVA
                     SLAERMRNGAKT"
     gene            3305..4744
                     /gene="gltX"
                     /locus_tag="PLG98_02635"
     CDS             3305..4744
                     /gene="gltX"
                     /locus_tag="PLG98_02635"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOE32176.1"
                     /translation="MVRKPRVRFAPSPTGELHVGNARTALFNWMFARHHGGSFVLRIE
                     DTDASRSALSYQTNLYDDLKWLGLDWDEGPEKGGHFGPYRQSERVAVYREFLQKLMKA
                     GAVYPCYCTEEELEEERQTLILSKRMPRYMGKCRNLSPEERNRKEKEGRKPSYRFKVQ
                     SQIIEFEDLIRGNVKFNAEDMGDFIIVRSNGMPAYNFACVIDDHLMDMTHVIRGEDHL
                     SNTALQIMLYRAFGFDSPVFAHHSLILGRDRTKLSKRHGSVSIGEFRRQGILPEAMIN
                     YLGLLGSSFTGAREVLSRDDMIQDFSLERASRSGAVFDEEKLHWLNAIYIRNTRTDDL
                     VERLKPFLDEPAYQNEMQNPEWFRKAVELVKTDLTTLADLKNHISIFFDSQYRISAEA
                     KDLLDQEPARKVVEAFASYLEKQDAGNQNLYAEAVRFAREATGAKGRALFMPVRAALT
                     GLLHGPELDKIFDVLGRNAALRRLKYFER"
     gene            complement(4741..5133)
                     /locus_tag="PLG98_02640"
     CDS             complement(4741..5133)
                     /locus_tag="PLG98_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOE32177.1"
                     /translation="MSFNLSRNPVYHILADILVAAHFLFILFVVFGGLLVIRKPSFAW
                     VHLPAALWGAVVEIFGWICPLTPLENHLRTLAGESVYGGDFIARYLLSLIYPENLTPS
                     IQKGLGAVVIVINLIFYFIAFKKNRARR"
     gene            complement(5123..6238)
                     /locus_tag="PLG98_02645"
     CDS             complement(5123..6238)
                     /locus_tag="PLG98_02645"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOE32178.1"
                     /translation="MKKIFAAVALIVVLLTAYLTLWPVPVDPVQWIPPLSDGYSGPHA
                     VNTGLAGLRMISLGREEGPEHIVIGKDQKLYTTVAGGKILRMNQDGTQQEVYADTGGP
                     VLGFDFDARGNIVAADVDRGLVLISPDKNITVLTDNVNGDPIRFADAVAVSQSGKIYF
                     TDASTRFAPKKWGGIFPASLIDILEQRSTGRVLEYDPAAQTTRIVAKGFSFANGVVLS
                     EDENWLFVNETGKYRVWKISVTAQDLDIASPGESAVILFENLPGYPDNLMRGLHGKIW
                     LGFAKPRNPAIDQRVITSTPFMRKLTYRLPKFLWPLPKPYGHVLAFTEDGRIVADLQD
                     PGGRYPETTGVTETADRLYIQSLHAKGIGWLPKDNVF"
     gene            complement(6284..7855)
                     /locus_tag="PLG98_02650"
     CDS             complement(6284..7855)
                     /locus_tag="PLG98_02650"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOE32179.1"
                     /translation="MSEDVQKNSTQEKINHFEKKNRTLKQMGGEKQIARQHELKKLTA
                     RERLDLFFDQDTFQEVQLFVEHRSTLFGLDKKDIPADGVVTGFGKVNGRTVFVAAQDF
                     TASGGSLGEMHAKKIWKVMEMALAAQKPFVSLNDSGGARIQEGVPSLEGYGGIFYRNT
                     LASGYIPQITAIMGPTAGGAVYSPALSDWVFMVKGSSFMYITGPDVVKAVIGEEISHD
                     ELGGALAHAAKSGVCHVVTESDTDCIEKIKKLLAYLPDSCHAPLPLSDCTDPADRTCP
                     ELDSMIPDKSNRAYDMKKIIKSVADNGELFELLELWAQNIITAFIRVTGRPVGVIANN
                     PRFAAGVLNVDASDKAARFIRFCDAFNIPLLTFTDVPGYMPGTHQEWAGIIRHGAKLL
                     HAYSEATVPKITVITRKAYGGAYIGMCSKQLGADYVMAWPTAEIAVMGAEGACNIIYR
                     NDIAGADNPTARRAQLVEAYEQQFNNPYFAASMGIIEEIIPPRDTRKKIIALLDALGN
                     KKVTGLSKKHNNIPL"
     gene            complement(8015..8548)
                     /locus_tag="PLG98_02655"
     CDS             complement(8015..8548)
                     /locus_tag="PLG98_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE32180.1"
                     /translation="MSFKKPYPAQYEKLLALKNGKNVFVRPIRDTDGKLLLELFDKLY
                     PDSIYRRFLTPLRMIPENLLFKLTHVNYDSDFALVAVIPENGKDAIIAVARYGYDVRE
                     QVTDFAILVRDDWQGLGLGKRLLKDIFAIGRDHGVSCFVSLIDSNNHVMKELIKRLDY
                     PVTYSYRTGASKVQIFV"
     gene            8881..10983
                     /locus_tag="PLG98_02660"
     CDS             8881..10983
                     /locus_tag="PLG98_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOE32181.1"
                     /translation="MEKLFYPESIAIIGLSSKPQNIPRLVLENLIRWGYKGRIFGINP
                     RAADSHVDGIKMYKSVQELPVVPDLVYVLIPARMIPDVMDSCGRAGVKRLAIPTGGFN
                     ELGEEGRKLSELVLQKAREYGIRFVGPNGVTVANTANGLCLPFVPSYSPPKGGLSIIS
                     QSGGVGLMLWNLMTDENVGMAKFASIGNKLDLDEVDFLEYFGRDPETKVIGMYLESVP
                     RGDRLIAAASKIDKPVIVLKSNTTAAGRKAAMSHTAALANNEDIIDAAFERSGIIRIH
                     QFNDFISMAKVFELPPMRGKRMMVMSPAGGFTVMMADLCEASGFEFADPGADFYDKLK
                     NSSNAGVINFSNPLDMGDIYDMNAYPQIFFSVMHNENVDGAVFVTQWPEMPRGEDVFY
                     KMFHTDLSKEVAGTILSSGKPLGVCLFGLSATISRIKQNLDFPIFNNASEMVHAFKKQ
                     YDYYAKKARGETGYPLPDGVDIEAAQKWIKGHAGVRGEESLELLSRFGIHVAPSDVAK
                     NGKEVLALADTIGYPVVLKVVSPDAVHKSEAGGVITDVQNAEEAVKAFDQIRSNLFRY
                     NAHADFQGVRVMKQASDGYDMFIGGKFDESFGPVVFFGYGGIYIEVFQDTANLLCPAD
                     CHEIEAGVKRLKSYKILQGMRGKGAGDIAGYVAMIERVSHLLHQFPQIRELDINPVRV
                     LADGSGVLALDARVRIDT"
     gene            11022..11759
                     /gene="truA"
                     /locus_tag="PLG98_02665"
     CDS             11022..11759
                     /gene="truA"
                     /locus_tag="PLG98_02665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOE32182.1"
                     /translation="MRNFKITVEYDGAAYCGWQRQHNGIAIQQLLEEAIKKITGQNVS
                     VIGSGRTDAGVHAMNQVGSFKCDTTLPVQKIFMGVNSVLPPDIVVKNLEERSDDFHAL
                     RDVKSKVYVYRICNQRLRPVLGRNYFWHVRYPLDIASMRKAVRLIIGTHDFSCFCAAG
                     TDVKDRVRTIVDIEIMTGEEGLIEIQVESHGFLKYMVRNIIGTLIEVGRGKRQPEEMK
                     LIIESKNRNIAGATAPACGLFLKEVKY"
     gene            11762..12673
                     /gene="rfbD"
                     /locus_tag="PLG98_02670"
     CDS             11762..12673
                     /gene="rfbD"
                     /locus_tag="PLG98_02670"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOE32183.1"
                     /translation="MRILLLGHKGMLGNDLFLRLSAEHELIGMDQEDIDIVSDNDCCQ
                     AIKDVSPDMVINAAAYTNVDGCETDRETCFAVNAEAVKNIAGACRQKKIRVLHFSTDY
                     VFDGTARKPYREDALCNPVNLYGASKLAGENYLRTLSDNYLLIRSSWLYGINGKNFVQ
                     AILDKAKAKEFIRDTMNRAKAVAEAPSTLSVVDDQVGSPTYTKDLALAVAFLIRKNAT
                     GIVHVTNRGSCSWHQFAVRILKSAGYGHIVVEPIKSSELQRPAMRPAYSILSNKTFIE
                     ITGKAMQPWQLALEDYLNNFKLKKSHS"
     gene            12965..13894
                     /locus_tag="PLG98_02675"
     CDS             12965..13894
                     /locus_tag="PLG98_02675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE32184.1"
                     /translation="MKKAMLVHLNELDFYLKKIDAVWPVRCLTGEAIDCEKVVDYYRQ
                     SRIVYKYVHSYSGAMHMAVNSDGVFNAKGFYTSLNEIVEIISPLPAGAVLELGCGKGF
                     NSAYLAKKIPHAHFTGIDVSGEHLAIARKKASRIHNLSILYGDFQDIMFPDGAFDTVF
                     ELESICHARKVRQVLSEVFRVLKKGGRFIVYDGFRQSGFHRLPDRLIEAARMTEKALA
                     VDHFEDIDGWLAMAVAEGFRIHEKKDLSASIMPNLARLQYLARRYFEYPMLTKLLLRL
                     LNVYTMRNCIAVLLMPFTLHNNAHGYYKIILEK"
     gene            13957..14220
                     /locus_tag="PLG98_02680"
     CDS             13957..14220
                     /locus_tag="PLG98_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32185.1"
                     /translation="MQVNLTEQEIVKFAYVTKEIIEQSWGATLSNAQINCYIECFYTL
                     KDLLEQIDREYLQKHSEISDYFLTGKRLINEKDKLQWEKEWQV"
     gene            14339..15832
                     /locus_tag="PLG98_02685"
     CDS             14339..15832
                     /locus_tag="PLG98_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32186.1"
                     /translation="MKIVLLSMPDSIPMIMHESAFHLPNCGIASIGANLDEGHDVYVV
                     DLIRKRRHLAGYLKRLLLKIHPKLVGLSAMAWQYETCIEIIRFIRLLLPEVKIAIGGY
                     HATLMYEEIAASPESGLIDFMIRGEGEEAFRRLVNALEGSDQLQDIPSLSFKRRGTFC
                     HNPRGALLDLSRLKIPIRDKRRLTSGYHAVFSKIEVIETSRGCTRTCSFCSMREMYGQ
                     TFRTFPIERVTADIDDIYYKHNTRAIFIVDDNLVLDPERVIDLCDAIIAKNYKGLSLH
                     VQADCISIAGNETMVRKMSLAGVKTVFLGIENVVKKNLMASGKGDIVDLSRKAVRICH
                     QFGIMVIAGLIFGFPDDDENDIVDNYRFLKSLDADIPYCQILTPYPKTPVRQQLLERG
                     MVTNLHDYKLYNGLRANIKTRHLDTQQLQYLVWLHRQKMFGWWEPSRLMRIKGGLWVT
                     FWTHLLRPFLRSTVGRQQAQKSWKELYERDVKYLDGLNQFKNLARNY"
     gene            15894..16451
                     /locus_tag="PLG98_02690"
     CDS             15894..16451
                     /locus_tag="PLG98_02690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE32187.1"
                     /translation="MKLVIRGGSIAAGYGVTKSYVDILTQALLPEGVCVINRARYRET
                     SFDGIETYPQDIDRYEPDFLLIHFGVDDAFQCVYRSEFQENIVQMIRLSRKRLQPVIF
                     LATSHTFDNPNDMDAVHIFYRSLRIVASDLKCEFIPVHRSWNAYLEEHNLCSQDLTQE
                     DSRYPNEQGHQVIANAMMNVLGKFF"
     gene            complement(16481..17863)
                     /locus_tag="PLG98_02695"
     CDS             complement(16481..17863)
                     /locus_tag="PLG98_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOE32188.1"
                     /translation="MEKFFDPHSVVVVGASNTPFNLGATICNMLKDYLQYPGAVYAVN
                     SKGEKVNGYPGYSSILDLPESPDLAVIIVAAKNVPRIIGDCARKGIKRVIIESAGFSE
                     GGSDGKAMQRKIDEIAKAHGIRIMGPNCLGTLDTRKKFCCFYGVNPSLVEMGRIFEAP
                     GNISFIIQSGGVVVLVIESFFYDLVGVNKVVSIGNKCDVDEADLIDYFQTDETQVIGL
                     YLESVTNGRKLMEAARKSRKPILIYKVGRTQEGALAAMSHTAGMANNDSIFNSACKQS
                     GMIRLQSIDELHSMPKMLTEMPLLKGKNIAMFTNSGAFGSIGADLLVDAHLQVVRLKP
                     ETQEKLKKAGQVFNIKNPVDIGPALPQTYLDIYEILLSADEVDGLLPLLSVWQTFVID
                     TLLELMKMCRHYQKPAAIYTPNAIAKSIGIRAKHRIPIFETAEQAVRALAVSGYHYDS
                     LQKRFPARAK"
     gene            18031..19824
                     /gene="mutL"
                     /locus_tag="PLG98_02700"
     CDS             18031..19824
                     /gene="mutL"
                     /locus_tag="PLG98_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOE32189.1"
                     /translation="MSKRIIVLSEDIANKIAAGEVVERPASIVKELLENAIDAGATDI
                     RVVLEKGGTQSIRVTDNGSGIDHDDVPLVFERHATSKIHQFEDIFNVVSFGFRGEAMP
                     SIASVARVEMLTRRKNDVSGTRAILDGGVIRAMDPAGTPEGTQISVSGIFAHVPARRK
                     FLKTEATEQGACLDVITRLALAHPEIRFKVEANGKEIFAAPEVHDTAKRIAMVMGGEF
                     SAHCMAVDAQQDAMTLKGFISRPEYSKSNSKSIYLFVNKRFIRDTSLTHAVLSAYRQI
                     IESRRYPAAALFLEVPPEDVDVNVHPAKMEVRFKNAHGVYELVSKTIAHHLSRAETSR
                     GHYLYRLAPAEKKDYFHRQPFVNEPSPSMPSRPADRSHAQQGIQNILLHRSQVSQPLA
                     ASEGDAGVPDKTILFGDMRYVGQYDGTYLLFEGPDSLILLDQHAAHERIILEKLKKDS
                     GKKAAGQSLLLPEIVNLTPGQMALLNDALDFLETIGLEIECFGRDAVAVKTVPTMLSK
                     VKISEMIYDIVDQLADQNRTPSLQERSDNLLASLACKAAIKANRLLSRDEVDALCREL
                     AETPFHATCPHGRPITIQFSLSEIERLFKRK"
     gene            19836..20771
                     /gene="miaA"
                     /locus_tag="PLG98_02705"
     CDS             19836..20771
                     /gene="miaA"
                     /locus_tag="PLG98_02705"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOE32190.1"
                     /translation="MKKLPLVIINSPTATGKTDLAVYLALECGGEIISADSMQVYRYL
                     DIGTAKPTMEQRRGIRHHLIDVVNPDGEFNAALFAARARAVIEELVHQGKPVFVVGGT
                     GLYIRALLKGIIDTPDVDASIRNHYRSLRETHGKIYLYDLLRQKDSTAAAQINPNDSV
                     RVIRALEVLEQTGESITVKQKKHSFSEDRYTTCKIGLRVERGELKERIEKRTDAMIRA
                     GLLDEVKGVLARGYRENLKSLQSLGYKQIIGFLNWQYDWRHCVNFIKRDTWQYARRQM
                     TWFSADKEINWFAPDALDILRRHVKIFQNENQETE"
     gene            20768..21409
                     /locus_tag="PLG98_02710"
     CDS             20768..21409
                     /locus_tag="PLG98_02710"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOE32191.1"
                     /translation="MTRRDHHTSVEYLCRLQRLFVVVLIALPLNIGAATHYHCADRSG
                     LQTFSDYPLEDQVCTEMVSQEETEIPKTDRPIAPNAESPEKKTTRIAVKENGILVPVT
                     LGYDGREADIRLVLDTGASATTIYDNVAGRLLVNLNNAPKAQGRVVGGGTIDANRVTF
                     SYLRIGPNTFRDWNIFVVENKDGDTSYDGLLGMDVLSTMRYKIDFRNQIIIWE"
     gene            21588..22835
                     /locus_tag="PLG98_02715"
     CDS             21588..22835
                     /locus_tag="PLG98_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOE32192.1"
                     /translation="MMNRKRDRIIFFTLLIFASLLTLAGCEPKVRVAVSQLDTPGHHT
                     YAGLKLLELEKYSDAIRAFKMATQLDSNYSKAYTGMALVGIATGKLSAAADDLEKGIL
                     NAKTSDEELFVNTAAIRYHTAKKSDEKWLEKAKYYFVETVMIDSQYAPVYYFMGIAYQ
                     EALEFDQAAKMFNKVIQLKAGHIEDANNRLEFLELVHKAHPATQAGKQIALKETVSRA
                     DIAMMLAEELKIKELYQKYLPKTAQEAQTEEEKVAASMSQAEHVAKEMEKMDQFHSYM
                     VLQEKEPVKLLANDIALHPLKNCIEAVLEAKVHGLENDPKGNFKPDEILSRGEFAIIL
                     EDILIKVSGERDIAARYVSSNSLFPDVPADMPYFTPIISVTSRGIMEAKNTKTGEFSP
                     LKPMSGVEALLAVKKLKENLKID"
     gene            22920..25577
                     /locus_tag="PLG98_02720"
     CDS             22920..25577
                     /locus_tag="PLG98_02720"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOE32193.1"
                     /translation="MAKRELGKAFEPAAAEKRLYEYWLKNKYFHAQDKSEAEAFSIVI
                     PPPNVTGMLHMGHALNNTLQDIIIRFKRMQGFNTLWMPGTDHAGIATQNVVEQQLLKE
                     GTSRHDLGREKFIERVWEWRRKYGGVIINQLKRLGCSCDWERERFTMDEGLSHAVREV
                     FVRLFNEGMIYQGDYIVNWCPRCHTAISDLEVEFKSDASFLWNIRYPFAEGQGEIVVA
                     TTRPETMLGDTAVAVHPDDKRYKNLIGKYVILPLMNKKIPVIADDYVTMDFGSGAVKI
                     TPASDPNDFAMAQRHHLEIISVIDGKGIINENGGAYKGQDRYEARKNIVNDLQKGGYL
                     VGTEPYAHNVGQCYRCKTDIEPMVSKQWFVKIKPLAKQATAAVIKGKTKIVPKTWEAT
                     YFDWMNNIRDWCISRQLWWGHRIPVWYCDNCKKVIVSTVDPDTCPDCGGASLKQDEDV
                     LDTWFSSALWPFSTLGWPEKTAPLKTFYPTSLLITGFDILFFWVARMMMMGLYVMKDV
                     PFKHVYLHALVRDEKGEKMSKSKGNIIDPLLMIDQYGTDAFRFTLAAYTAQGRDVRLS
                     PERIEGYKFFVNKIWNASKLTFANLDDFDETATAEVADSLADLWIKARLNKAVEDVTT
                     ALDEYRFNDAAAAVYRFIWHELCDWYLELSKPVFYGKGSAEQKQAAQKTLGVVFPTML
                     KLLHPFMPFLTEELWQVFHGDDKNSIMISRFPAVCSEWKNDAAEKEMETIMDMITAVR
                     NIRGEMRIAPSQKLDVLMSTVDEKTQKMVEKHKDYILNLANLESLTVEINAVEPKGVA
                     TAVVGSTKLFVPIVGIVDLSGEIARLEKELAKISKDLQQSSKKLANRDFMSKAAPDII
                     QKEEDKVKDFQKKHTTLENALKKLKGMSA"
     gene            25577..26413
                     /gene="nadC"
                     /locus_tag="PLG98_02725"
     CDS             25577..26413
                     /gene="nadC"
                     /locus_tag="PLG98_02725"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOE32194.1"
                     /translation="MEQRIVRIIQNALAEDIGSGDITTGATVNARKKGRAVAIAKGNF
                     IVAGTDVFAGTFRTLDKNIKVQKRIPEGYPVKKGDIIAEVSGRLDHILMAERVALNLF
                     QRMCGIATLTHRYVRAVRKTKAGILDTRKTVPGLRVLDKMAVKTGGGQNHRFGLYDGV
                     LIKDNHIEAAGGITAAVNAQRKKLSRFMKIEVETRNLDEVREALECGADIIMLDNMSV
                     SAMKRAVSFVAGRALLEASGNVGLKNVMDIAATGVDFISVGELTHSVQAADISLKIRS
                     SS"
     gene            26431..27243
                     /locus_tag="PLG98_02730"
     CDS             26431..27243
                     /locus_tag="PLG98_02730"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOE32195.1"
                     /translation="MDWNIARLQETLGTGLFGGHLHFYPEIVSTNDEAIALAMAGAPE
                     GTAVLADAQTAGRGRWRRSWISPPGVNIYTTIVLRPECESSGASQIPIMAGVAAAEVL
                     DGVCPGRVKLKWPNDVIIGPKKVCGILSQAKITSKKIDFAVVGIGINVNAGVHHFPKT
                     IRESATSLLIESGGEISRHDLLISLYENLEKWYKQLLQKGFEPVRKKWLESAAMMGRP
                     IRIAFKGESTEGIAKGLGDDGCLILVVEGNEEIKVSAGDATIMRWKENAFGD"
     gene            27227..28003
                     /locus_tag="PLG98_02735"
     CDS             27227..28003
                     /locus_tag="PLG98_02735"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOE32196.1"
                     /translation="MLLVIDVGNTNTVLGLFDGKNLIHDWRIRTEIDHTIDEYGVLIY
                     NLYQSSRKKEKAFKTVSAIIISCVVPPMLNILEPLCIKYFNVKPLIVGPGIKTGMPIF
                     YDNPKEVGADRIVNAVAAYNKYRKEAIIVDFGTATTFDYVSPKGEYMGGCIAPGIVIS
                     SEALFEKASKLPRVEFSRPKSAITKDTVSAIQAGIMFGYAGLVDGIVNRIRTEVETKP
                     LVIATGGLANIIAPETKTIEKIEEMLTLDGLRFIYELNKK"
     gene            28006..28623
                     /gene="coaE"
                     /locus_tag="PLG98_02740"
     CDS             28006..28623
                     /gene="coaE"
                     /locus_tag="PLG98_02740"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOE32197.1"
                     /translation="MLNVGLTGGIACGKSTVAKMFVEKGAHLIDFDKLAHRLQEPGQP
                     AWQKVVDYFGRQILRPDQKIDRVKLGRIVFADKKKLRVLNKLVHPPVYREWQLRLEKI
                     GRKEKHAIVLSDVPLLFEGKKTHLFDLTILVLSDPEDQICRLISRNRISGEDARARLK
                     SQMPIGKKTALADIVIDNRDSIARTKKTVDRVWRKLVRLEKTKSA"
     gene            28669..29286
                     /locus_tag="PLG98_02745"
     CDS             28669..29286
                     /locus_tag="PLG98_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOE32198.1"
                     /translation="MIEKNVKTDFSSKISEPVIGAGTYVHPLAAVIGNVILGENIMVA
                     PTACIRGDEGQPLHVGDDSNVQDGVIIHALETELDGKPVEKNQRDVDGKKYAVYVGKR
                     VSLAHQVQIHGPAVVMDDTFVGMKTLIFKSFVGKHCVIEPGVTLMGVTVDDGRYVPAG
                     SVVKTQADADVLPVITPDYPMKEMNKGVLHVNKALAKGYLAAAKK"
     gene            29430..30056
                     /locus_tag="PLG98_02750"
     CDS             29430..30056
                     /locus_tag="PLG98_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOE32199.1"
                     /translation="MKCRQNLRQKLNLKNGLLQALNTKYATFYFSKTMIILDVVPYGS
                     REIFRYAIRRRAGNTPAIDFSFMTNNLGKAWWAVSDTTHRNYLASQHVLKMAKRYTIT
                     DKSQRPKIVKIPAVKVSALNCLDIKKIDLASCDLDRVIKSAYRRQVKIYHPDLGGKAA
                     DFRKIHSAYKELLAWADHPVFVHRRGFPDKWFYSAETQKWVQPTPLSD"
     gene            complement(30067..30651)
                     /locus_tag="PLG98_02755"
     CDS             complement(30067..30651)
                     /locus_tag="PLG98_02755"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOE32200.1"
                     /translation="MQENVTGLILAGGKNSRMGVNKAFLEIDGKRLMDRTLSVYRRIF
                     PKIIIVTNDPLAYVEFSEAMIVTDIYKGKGSLGGIYTGLFYAASDDCFVAACDMPCLN
                     EDFIRYMIGRRRSYDIIVPELSEGFQALHAIYSRRCLAVMKKMILADRLKIAGLFKDM
                     HTLRLTEEDIRPFAGNTPLFTNINTPQDLQSFRK"
     gene            complement(30663..34931)
                     /locus_tag="PLG98_02760"
     CDS             complement(30663..34931)
                     /locus_tag="PLG98_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOE32201.1"
                     /translation="MQTLGINIGSSSIKLILLEDDRIVWQKVEPHEGNFVHALEKTLS
                     ERSFPPGVLTLATGTEGRNLLNVNSVIEPLCIEEALRHLNGNVNALVSLGGEDLVVYS
                     IDENQQIITSFSGNKCASGTGEFFKQQLGRMNMHLNDIHSIPEESRILKLSSRCSVFM
                     KSDCTHRLNKGEASTGDIVLSLSDVMATKVIDFIKKAKIAGKKVLLVGGVTRNPHLIR
                     FIRQRMPENDFIVPDQAVYFEAYGAALLAGSMGTPLPALNDLFKTHMVQFKRFKSLKT
                     AEGKVHYLPSKKSKVIAGREYILGVDGGSTTTKACLIDMETSEITASFYGRTHGDPVK
                     ALKNCFLEMQKQIREDIGDRAIKITLAATTGSSRELLGVFLETPGVYNEIIAHAVGTT
                     FYQEDIDTIFEIGGQDAKYVYLKNKVPIDYAMNEACSAGTGSFLEESAQGDLNIHHAW
                     EIGDIALGASEPLKFGEHCSAFINSDVRKAIQQGASREDITAGIVTSIVSNYLNRVVG
                     NRTVGQNIVLQGGVAKNKAVPLAFAMLLDKNILVPPDPELMGCFGVGILARQKLHEGF
                     LSRRDYDIEKILSTEILHEKEFQCSACDNFCPIRVLNVNGRKYMFGGRCNKYANIRKK
                     KSFDESDVVDYIEQRNDLLFNQCAPRPADLIRKKDFVVGIPKCFSVYSHWPLYAWFFH
                     TLGVETVLSSDITHEGVARVESTYCFPAEIAHGAVQDIFNRNVDYIFLPHFRDMESYE
                     SNTPACFCPITQSLPYYIKKAFPEIPEGKYLAPVVSFMYGTQKASESFIAMAVRLGFD
                     QEDARLAFSVALEKQMECFRKFAELGKIAIENSRKSQRPVIAVLGRPYNAFTPDANMG
                     IPRKYTSRGFTVVPFDILPFEDQGIYPNMYWYYGQQDMKAAALLKNEDNIFVTYISNF
                     SCAPDSFMLHYLKWIMGMKPFLVLELDSHTADAGVDTRIEAFLDIIEGYRSKISDIRE
                     SRYDNGLRFINTSGEDIHIRNTLTGEKIPIKNNPRVKLLLSNMGRLSVEVLAATIRGL
                     GIHAETMPVPDIYTLQMARNHASGKECLPSHLVLGSTLKYLSSEKYRKDEIYILFVPT
                     TTGPCRTGQYFVFYENLFKDLRLENVVVFTLDADNSYNELGTEFSKYAWWAVLIGDYM
                     KDIETSLRTCAADPVKAVSDYNIIWQKLIDVAEKDVTQSLPVLKEISSSIAKIPLKKK
                     IDDCPKVLIVGEIYVRRDDFAVDELIRHFSRRGIIGKVSNVAEWVYYCDFVRRHELQK
                     RLKLVPWYKKFLAAEFSDIVKWKIEHVYKKKVEKNIRDILSTTGLVPHAPHNMDEIME
                     NTEKHFVNNELYSEISISSGVAATAMMDGYAGIVNISPFACLIGRVIEGVLTPWARER
                     KYPAISIEIDGNLFPPNVISKLEIFMLNVMRFRNQDETDHRTTL"
     gene            complement(35165..35836)
                     /locus_tag="PLG98_02765"
     CDS             complement(35165..35836)
                     /locus_tag="PLG98_02765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOE32202.1"
                     /translation="MEPLIFFHTQWLKDVGIDMTIVFNTLFVMALLAFFSFLATRRTE
                     VYPGKTQNVMEVIIGGFDGLLNEIMGHNGRKFFALISTLGLFILVSNLIGIVPGFESP
                     TSNINTNAAMALIVFLSTHVVGIKEHGLKYFKQFMGPVWWLTPLMLPIEIISHISRPL
                     SLTFRLFGNIKGEDIVLLVVLFLVPWFVPLPVFVLMVFTSVVQTLVFMLLAMMYIAGA
                     MEEGH"
     gene            complement(35843..36238)
                     /locus_tag="PLG98_02770"
     CDS             complement(35843..36238)
                     /locus_tag="PLG98_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOE32203.1"
                     /translation="MNLIVKDPLQKRIEITNWIILAVIFIPSLFLAPLKFSLGILLGG
                     FVSIVNFHWMARGLRGIFQNLQGNPKGTVMVKYYIRLAIMAVVLYLLIANNVVDIIGL
                     LIGLSVVIINILATMIIALAKKNFIEEVV"
     gene            complement(36198..36482)
                     /locus_tag="PLG98_02775"
     CDS             complement(36198..36482)
                     /locus_tag="PLG98_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOE32204.1"
                     /translation="MDKETRKYAMQMAFASSIGIAMILAVFGCLLLGSYLDKKFDSGH
                     VFTVVFLFIGLAAGFRNLYVLVQKNFTDEETIIKSLKSEPHRQRPAPKKD"
     gene            complement(36542..37486)
                     /locus_tag="PLG98_02780"
     CDS             complement(36542..37486)
                     /locus_tag="PLG98_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOE32205.1"
                     /translation="MLPQKRITITGARGFLGTHLLKKFKSKGCRNIEIADLPEYNLTD
                     INAIRRMYEETKPDVVIHLAAKVGGIGFNQEKPAELFYDNLIMGAQLLHEAHIRKIEK
                     FVALGTVCAYPKFTPTPFREEDIWNGYPEETNAPYGLAKKMMLVQSQAYRQQYGFNSV
                     FLLPVNLYGPGDNFDSRSSHVIPSLIKKCFEAIDRQSDQLEVWGTGSATREFFYVEDA
                     AEAIYLATLLYDKSDPVNLGSGFEISIKDLTTLIAELTGFSGRIVWDSSKPDGQPKRM
                     LDTSKALKEFGFIAKTDIRTGLTKTIDWYKKNRHLLKK"
     gene            complement(37497..38528)
                     /gene="gmd"
                     /locus_tag="PLG98_02785"
     CDS             complement(37497..38528)
                     /gene="gmd"
                     /locus_tag="PLG98_02785"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOE32206.1"
                     /translation="MKKAFLTGITGQDGSYLAEFLLHKGYEVHGLIRRSSTFNTERID
                     HLYHDFHDPEARLFLHYGDLSVSAQLMDLLHSVKPDEIYNLGAQSHVRVSFDMPEYTG
                     DVTALGTLRILEAIRKTGITTKFYQASSSEMFGAAPPPQHEKTVFQPRSPYAAAKVYS
                     YYITQNYRDAYGIFAANGILFNHESPRRGETFVTRKITRAATQIKLGLKDKLYLGNLE
                     AKRDWGFAGDYVEAMWLMLQQNTPDDFVIATGETHSVREFAEMVFQKLDLDYKRYVEI
                     DKRYFRPTEVDALLGDSTKAKKILNWQPRVSFENLVDMMIASDMELAQKEKTLLDAGF
                     VSNNQNFNH"
     gene            complement(38602..39885)
                     /gene="hemL"
                     /locus_tag="PLG98_02790"
     CDS             complement(38602..39885)
                     /gene="hemL"
                     /locus_tag="PLG98_02790"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOE32207.1"
                     /translation="MKETTSVQFSKANRVIAGGVNSPVRAWKSVGGHPRFVLKAKGSR
                     IYDTLGKAYIDYVLSWGPMILGHAHPDVTAAVRAALKQGTSFGAPTLLETAIAEMISS
                     ALPSIERIRMTSSGTEAAMTAIRLARGYTGRKLIVKFDGCYHGHSDSVLIKAGSGPTT
                     LGIPGSPGVPDDIARLTLSLPYNDTIALAKTVKRYGSQLAAVIVEPVAGNMGVVAPRE
                     EFLKQLRVLTAKNGIVLIYDEVITGFRFTFGGYQNLVGITPDLTCLGKIIGGGLPVGA
                     IGGRKKIMERLAPTGDVYQAGTLSGNPVAMIAGLTTLQLLKAKKKSFASLDKKTDQLC
                     RAMHEMFFKKGIPVCINRVFSMFTVFFQEGPVVDLQTALKSDTNMFARFFRGMLKNGI
                     WLAPSQYEAGFLSFAHTEKDLQKTLQACAKTLRNL"
     gene            complement(39885..40352)
                     /locus_tag="PLG98_02795"
     CDS             complement(39885..40352)
                     /locus_tag="PLG98_02795"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32208.1"
                     /translation="MRKLTSKQKKIARLLQKDIPVTKFPFKKLSGAPTPGAKEILTAL
                     DQFREQGLIRKFGAILRHQKAGYGQNALVLWSVPPSQTETTGRLFASFSFISHCYERH
                     PAFLERYNLFTMLHSDGRSISSLIKDMVNATGIGDYLILKSLKEYKKTSPEYF"
     gene            40550..40927
                     /gene="atpE"
                     /locus_tag="PLG98_02800"
     CDS             40550..40927
                     /gene="atpE"
                     /locus_tag="PLG98_02800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOE32209.1"
                     /translation="MKKSLMYVMLAMVMVIVSAPFVFAAPEAAAQAPAGTVDYTKAIV
                     ASIFALAAGLAIGLGAIGAGLGLGQATSGASNAVGRNPEAQGKVMLTMMVGMAMTESV
                     AIYALVISLVILFANPLLKIIGM"
     gene            complement(41089..41469)
                     /locus_tag="PLG98_02805"
     CDS             complement(41089..41469)
                     /locus_tag="PLG98_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOE32210.1"
                     /translation="MLQDRMKSLGVAETDLEETFIRSSGPGGQKVNKSSSCVYLRHLP
                     TGLSVKCQKERSQSLNRFLARRLLLDKIEIQQKGLMAEARQTIEKIRRQKRKRSKRAK
                     EKILTDKHQQAEKKILRAKVSVEE"
     gene            complement(41518..41949)
                     /locus_tag="PLG98_02810"
     CDS             complement(41518..41949)
                     /locus_tag="PLG98_02810"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32211.1"
                     /translation="MPVKQISVILNNVPGSFAALTHILDLEDISTKAVSAASIADDSK
                     VRLVVNDPDRAAAVLKSYNLNFEVTPVLAVEVPLHAGGMNAILKPLAKAEINIHYLYT
                     TINRFGEETIVILGVNKIDEAREILEQNWIHLIDEEIYSIP"
     gene            complement(41960..42502)
                     /locus_tag="PLG98_02815"
     CDS             complement(41960..42502)
                     /locus_tag="PLG98_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOE32212.1"
                     /translation="MLIKTIFSGFGGQGVLMMGVSFAHSGMSQGYQVTYLPAYGAEMR
                     GGTANCTVAIADEDIASPVASEPDFLVAMNSPSLFTFQNKISSGGTIFLNSSIIKEKP
                     HRSDIKVCSMPCADMAQDLGNIRVSNTIMMGAFIKVSAVIPPDVYLKSLETIMGSKKK
                     SLAELNRKAFNAGYEYVKND"
     gene            complement(42502..43272)
                     /locus_tag="PLG98_02820"
     CDS             complement(42502..43272)
                     /locus_tag="PLG98_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOE32213.1"
                     /translation="MVKTVFKRPRSLKENVFHYCPGCGHSIAHRIIAEVIDELDIRGI
                     TIGVPPAGCAVLAYNYFDVDMIEALHGRGPAMASAIKRSLPDRVVFSYQGDGDLAAIG
                     IAESFHAANRGENITIIFINNAVYGMTGGQMAPTTMLEQKTTTSPAGRHRTLDGYPVK
                     VSEIFSQLEGVTYLERCMVNSPLNINKTKKAIKKAFQCQLDGFGFSLIEILSPCPTNW
                     KMTAFNSCKWIDEVMSKTFPPGVYKDNIAAAKKSAEGR"
     gene            complement(43293..44351)
                     /gene="vorB"
                     /locus_tag="PLG98_02825"
     CDS             complement(43293..44351)
                     /gene="vorB"
                     /locus_tag="PLG98_02825"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOE32214.1"
                     /translation="MNKVLMSGNHVIGEAAIRAGCRFYAGYPITPQNELTEYMAEHIR
                     HAEGGIFIQSESEVAAINMIAGASAAGLRVMTSSSSPGISLKQEGISSMAACELPGVI
                     VNIMRGGPGLGNIRPSQGDYFQATRGGGHGDYRTIVLAPASGQELSDLTMEGFDLADK
                     YRNPVMILADGMMGQMMEPVLFKKPKPRKYSEKFMLRGAGTGKSKFIHGLILDPIDME
                     EHNWKLARKYEVITQKEPRFEEHKIEDAKMVIVAYGTAARIAKGAIKRLRAQGVKVGL
                     FRPVTLWPFPELRLRSLSEIVKHYLVFEMSTGQMLEDVRLSLQGYADIKFHGRPGGAV
                     PTPAELANVIAKFYEKID"
     gene            complement(44344..44553)
                     /locus_tag="PLG98_02830"
     CDS             complement(44344..44553)
                     /locus_tag="PLG98_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE32215.1"
                     /translation="MKGYIVINKEICKECLLCINFCPKGHIVTTTEYNALSYHPVGID
                     ETKECNGCGICATMCPEVAIEVYRE"
     gene            complement(44663..45748)
                     /gene="ribD"
                     /locus_tag="PLG98_02835"
     CDS             complement(44663..45748)
                     /gene="ribD"
                     /locus_tag="PLG98_02835"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOE32216.1"
                     /translation="MNDEYYMKIAIGLARKARGCVSPNPMVGAVLVKNGRIIGKGYHQ
                     CFGGYHAEINAIQNAAGRVGGSTLYVTLEPCCHEGKTPPCVDAIIHNKIARVVIGTID
                     SNPLVCREGVNRLIHSGIDVKTGILEEECRLLNETFFHYMETGLPFVTIKYAQTLDGR
                     IATSTGNSQWISSPASLRLSHRLRAEHDAILVGRQTVERDNPELTVRLVRGRNPLRVI
                     VDSALKVSPDARIFQNLSAAPTLVATLKTASDSRYKKLAALGVTMLTVSADRDGRVDL
                     KKLFKRLAAKGISSVMIEGGAQIITSVLKRNLANRLVTVIAPKIIGCGIEAVGDLNIR
                     HLDLAMKITVKKISVRGHDIIVDGRLM"
     regulatory      46004..46094
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            46174..46944
                     /locus_tag="PLG98_02840"
     CDS             46174..46944
                     /locus_tag="PLG98_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOE32217.1"
                     /translation="MMSFVMNTLAFVFGAAIGSFLNVCIFRLPAEKSIVAPASRCPYC
                     LHPIRFYDNIPIISFILLGGKCRDCESRIAWRYPLVELTMALLSVALFMKFGLSLNFF
                     VFFIFTAVLMAITFIDLDHQIIPDILTLPGIPLFFLAAVFIVRIPWLEAFLGFLIGGG
                     VLFVIALGYEFIAKREGMGGGDIKLLAMIGAFLGWKSLIFILLFSSLTGAVIGIAAMV
                     IHKQDTKYAIPFGPFLSAAAIAYIFFGQCYMNLFLMGY"
     gene            47150..47659
                     /locus_tag="PLG98_02845"
     CDS             47150..47659
                     /locus_tag="PLG98_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE32218.1"
                     /translation="MMKTLKNPMNRGLSLVELLIVIAILGIVGTIASFGWSRYVNNAD
                     LRTLSREITSDIAKMKQRAIAEGVCFQMTINVAGAGNSYTLERANTASCSGASTVFTV
                     LQTKKPEELGLRAGINIKSTTHNGNDIEFQTRGTISNGRIELENSRGSQAIITSNITG
                     KTHVTYNMQ"
     gene            47637..48071
                     /locus_tag="PLG98_02850"
     CDS             47637..48071
                     /locus_tag="PLG98_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32219.1"
                     /translation="MLHTTCNNKGMGMVEVLIAIFLTSVAVLSILALQPSGWKAMAKS
                     DFAGRAAEVLYNTLEDYQVRISNPCNAVTTGNQAQQTVTTSGETDAIQGDITYTVDVN
                     IVQTNVDPQTFVVTVTVTWPGNTRGISESLSITRQEIYKFGC"
     gene            48075..48806
                     /locus_tag="PLG98_02855"
     CDS             48075..48806
                     /locus_tag="PLG98_02855"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE32220.1"
                     /translation="MMRNKHGFTLVELIVALFVGSLVLVAIYSTVNIAQRSSSGLERR
                     VVAQQDARSALELMAAEIRMASFNPLSRDNIWIDVNNFDSDCGGVSFVNQNYRGIRQA
                     AANAIAIEMDINGDGSIDDPNEIIRYRYDSANRYITRSTNCGGAQAFLGDTNANQNIK
                     TVLVENNNAGIPVFRYFDGSGTDISANVVANPADPLIGIPGVRRIEITLVADTSNADV
                     GSNNRRRIIYSTSVIPRNHFALIYE"
     gene            48811..49359
                     /locus_tag="PLG98_02860"
     CDS             48811..49359
                     /locus_tag="PLG98_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32221.1"
                     /translation="MFAKRKKYLKNEKGFALVAALIACLILLALGMLVINMSTGDLFT
                     TSAVIGNKKSLAAAESGISKIIADVDPDNWTTTEHYTAATDGSGNLCGTDAFNHGLYD
                     FIDITGGTDPNTEFAVCIPRPSAQPPLPASGYGLSGGGSSAVGYAYMRYDSTVVGRNT
                     SHDAEAKVDVGVGFGPVPLGGN"
BASE COUNT        12761 a        11651 c        12795 g        12278 t
ORIGIN      
        1 ctaccttgcc aaggtagacg tcgacggttc aaatccgttc gcccgctcca ttttattttt
       61 aaggcggcat agccaagtgg ctaaggcagc ggtctgcaaa accgttattc cccggttcaa
      121 gtccgggtgc cgcctccagg aaaattcagg agtttacttt ttttaaaaaa agtgactcct
      181 ttttattttt ataagttata tccgatattt aatcattcat gttgtgttta aaaaacggtt
      241 aaattatggt tttttagcca tgaaatattc aagtattact taaatacgga ttggttattg
      301 actaatagtt caagtagtgg tataataacc ctctgatatg gaaaggcagg cgaaaaataa
      361 aatgtttaaa gtaggcgatt tggcggtata tccggcacat ggtgttggag tcattgaagc
      421 cattgaaaat aaagaagtaa tgggcaaaaa acagtctttt tacgtgatga agatcatggg
      481 gaacggcatg aaaatcatga ttcccacgat gagcgtcaag gccgtcggct tgcgggaaat
      541 catcatggaa aaggaaatac ccaaggtgta cgaaattctt cgcaacaaag atgtcaccat
      601 cgataagcag acctggaaca aacgttaccg cgaatatctt gagcgaatca agacgggttc
      661 ggtatttgaa attgcggggg ttctgcgcga tttgtttatt ttaaaaagcg ataaaaatct
      721 ttccttcggc gaacgaaaga tgatggatac agccaaaaat cttctggtca aagaaatatc
      781 cgtggccacc aacgcgcagg aatctgaagt cgagcataat ctgcagaaga tttttaccgt
      841 tcagtagaaa aatcatcttc atagtcaatc aggctctttg aaaattgaaa aaagacaggt
      901 tactttcttt ttttagggat accgtcattt ttcctccgtc cttatattat tttttaaata
      961 aattttttat aaacaaattt ttttaacatt catgattcac agaaaggagg tgaaacggtt
     1021 gattatcaga attatattaa taatggcatg ttccatcagc ggttatgcca tcgcctttta
     1081 tacgtcttcc gattcttttc tgatccttaa atccattgcc ggctttatta tcggattaac
     1141 gattgccttg actgtcataa aaatagaaca gcacattcgt caattatctc tcaaaattat
     1201 catcggcggc gtcgcgggca tgattgtcgg actcctgatt gcattaatat ttagtttcgg
     1261 attaaatttt gtaagtaaaa tcagggagaa tcagcaggcc cttccctgga tttatctgct
     1321 gattacagga atattcagtt atctgggcct caccctgggt tcgaaaaaaa tcgaagaatt
     1381 cagctttggc ggcatcgatt ccaaaaccgc ccacgatcac cgtattctgg ataccagtgt
     1441 catcattgaa ggacgcattg ccgatatctg cgacagcgga ttcatcgaag gagaattgat
     1501 cgttccccga ttcgttttga atgaactgca gcaggtggcg gattcgtccg attcgatgaa
     1561 acgtctgcgg ggaaggcgcg ggctggatgt cctcaatcat ctgcagaaaa gtgccaacgt
     1621 caacatcgag attgtcgatc aggattttcc caagatcaaa ggcgtggacg gaaaactggt
     1681 cgctctggcc aaacagctca acgccaagct tgtgaccaat gattataatc tcaataaggt
     1741 ggccgaactt cagggcatca gagtgttaaa catcaatgaa ctggccaacg cgatgaagcc
     1801 ggtggtgctc cccggcgaac agatgaccgt gaaaatcgtg cgagagggaa aggaagccgg
     1861 ccagggcgtt gcctatctgg atgacggaac catgattatt gtggacaacg ctcagaaaat
     1921 gcttaatatg aatgtccggg tggttgtgac cagtgttctg cagaccactg cgggaagaat
     1981 gattttttca gaactcaagg aagtggctta agccgaatgc gtcatcggtt tttttaaaac
     2041 ccgggatcaa tctcccgggt ttttttatcg ctattccatt ttgataataa aaatgctatc
     2101 agcttcatga aaaataacgg ggaacaatcc atgaaaacgg tgctcatcat tcctgccggc
     2161 ggggcagggc aacgatttaa atcccacgtc gccaagcagt atctgctgct ggatgcgctg
     2221 cccgtactgg ttcatacgct gaaggttttt caggactctc cggttgttga cgaaatcatc
     2281 ctggtgttgc cgcaagacga tgtggtgcat gtccgccagg acctgattga aaaatacggc
     2341 atgagcaaag tggttgccgt tgttgccgga ggccgggaaa gacaggattc cgtcaaaaac
     2401 ggccttgacg cgatccaggg gaatgccggg ctgatcattg tccatgacgc ggtgcgtccc
     2461 tttgtgacgg atcaaatgat cggcagggtg gtggctgccg ccagggcctg cggagcggcg
     2521 gtgaccggcg tgcaggtcaa ggacaccgtt aaggcaacaa aagacgatca catgatccaa
     2581 tccacggtgc caagggaaaa tctgtggctg acgcagaccc cgcaggcgtt tcgcttcgat
     2641 atattgaaag acgcttaccg gagagcctat gaggaaaatt tttacgggac ggatgatgcg
     2701 gcgttggtgg agaggaccgg caaagaggtc aaaatggtcg aaggttccta tgacaatata
     2761 aaaatcacta ccagggagga tatgctggtt gcggaggcat tcatgaaaaa gcgaaacacc
     2821 gccggcatcc ggagcggctt cggttatgac agccaccggt ttacagaagg cagaaaactc
     2881 gttctgggcg gtgtggaaat tccctgcgac agaggactcg aaggccattc ggatgccgat
     2941 gccctgatcc acgcaatctg cgatgccctg ctgggcgccg caggcgcagg agacatcggc
     3001 aggcattttc ccgacaccga tccgcagtac aaagatatct ccagcatgat tttgctgaaa
     3061 aacgtcaaaa aaattttgga cgggaaaggc ttccgtattc agaatatcga caccacggtt
     3121 atcctggaag ttcccagact ggcgccctat gcggatgcga ttgtcgccaa tatcgccagt
     3181 gttctgaaca tttcggaaga atgcgtgaat atcaaagcga aaacgaacga aggcatgggc
     3241 tttaccgggc gcagagaagg cgtggcggtg tttgccgttg cttcgctggc agaaaggatg
     3301 agaaatggtg cgaaaaccta gggtcagatt tgcgccttct ccgacgggag agcttcatgt
     3361 cggcaacgcg cggacggcat tattcaactg gatgtttgcc aggcatcacg gcggttcctt
     3421 cgtcctgcgc atagaagaca ccgatgcatc gcgcagcgcg ctttcgtatc aaaccaatct
     3481 gtacgatgat ttgaaatggc tgggtctcga ttgggatgag ggaccggaaa agggaggaca
     3541 tttcgggcct taccggcaga gcgaacgcgt cgcagtttac agagaattcc tgcagaaatt
     3601 aatgaaagcc ggcgcggttt atccctgcta ttgcaccgaa gaggagctgg aggaagaacg
     3661 ccagacgctg attttaagca aacgtatgcc gcgatatatg ggcaagtgcc gcaatttgtc
     3721 gccggaagaa agaaaccgca aggaaaaaga aggaagaaaa ccttcgtatc gctttaaggt
     3781 tcagtcacaa attattgaat ttgaagactt aatacgcggc aatgttaaat tcaatgcgga
     3841 ggatatgggg gatttcatta ttgtgcgttc caacgggatg ccggcctata atttcgcctg
     3901 cgtaatcgat gatcatctga tggatatgac acatgttatc cggggcgagg atcatctttc
     3961 caatacggct ttgcagatca tgctctatcg ggctttcgga tttgattccc cggtatttgc
     4021 tcaccattct ttaattctgg gcagggatcg gaccaagctg agcaaacggc acggttcggt
     4081 ttcgatcgga gaattccgca ggcagggcat attgccggag gcgatgatca actatctggg
     4141 gcttctggga agttcattca ccggcgcccg ggaagtctta tcccgtgacg atatgatcca
     4201 agacttctcg ctggaacgag cctcccgaag cggcgctgtt ttcgatgaag aaaagctcca
     4261 ctggctcaat gcgatttaca tccgaaacac aagaaccgac gatctggtgg agaggctgaa
     4321 accgtttctt gacgaaccgg cgtatcaaaa cgaaatgcaa aacccggaat ggttccgaaa
     4381 ggctgtggaa ctggtgaaaa cggatttgac aacccttgca gacctgaaaa atcacatcag
     4441 catttttttt gacagtcaat acaggatttc cgcagaggcc aaagacctgc tggatcagga
     4501 acccgcccgg aaagtggtcg aggcatttgc ctcatatctt gaaaaacagg atgccggaaa
     4561 tcaaaatctt tatgcagagg ccgtccgatt tgccagggag gcaaccgggg caaagggcag
     4621 ggcgttgttc atgccggtgc gcgcagcatt aacaggtttg cttcacgggc cggagctgga
     4681 taaaattttc gatgtgctgg ggcggaacgc ggctttaaga cggctgaagt atttcgagcg
     4741 ctagcggcga gcgcggttct ttttaaaagc gataaaataa aaaatcagat tgatgacaat
     4801 taccactgca ccgagacctt tctgaatgga cggtgtcaga ttttccggat agatcaaaga
     4861 cagcaggtac cgggcgataa aatcaccgcc gtaaaccgat tcgccggcaa gcgttcgcag
     4921 gtgattttcc agcggcgtca gcggacagat ccagccgaaa atttccacga cagcccccca
     4981 gagtgccgcg gggagatgca cccatgcgaa ggacggtttc cggatgacga gcagaccgcc
     5041 gaatacaaca aaaagaataa acagaaaatg agccgcaaca aggatatctg ccagaatatg
     5101 atacacggga ttcctcgaaa gattaaaaga cattatcttt gggcagccag ccgatgcctt
     5161 tggcatgcag gctctggatg tagagcctgt cggctgtttc cgtaacaccg gttgtctccg
     5221 gatagcgacc gccggggtcc tgcaggtcgg cgacgatccg gccgtcttct gtaaaggcca
     5281 aaacatggcc gtagggtttg ggaagcggcc acagaaattt cggcagccgg taggtcagct
     5341 tgcgcataaa aggcgtggag gtgatgacgc gttgatcaat ggcgggattg cggggcttgg
     5401 caaatcccag ccagatcttt ccgtgaagac cgcgcatcag gttgtcggga taaccgggca
     5461 ggttctcaaa caggatgacg gcagattccc caggcgaggc aatgtccaaa tcctgtgcgg
     5521 ttacggaaat tttccagacg cgatattttc ctgtttcatt gacgaaaagc cagttttcat
     5581 cttcgctcaa caccacgccg ttggcgaagc tgaatccttt ggccacaatc cgggtggtct
     5641 gtgctgccgg gtcgtattcc aaaacgcgtc cggtcgagcg ctgttccaga atgtcgatca
     5701 ggctggccgg aaagatgcct ccccattttt tgggcgcaaa acgcgtggag gcgtcggtga
     5761 aataaatttt ccccgattgt gacactgcga ccgcatcggc aaaacgaatg gggtcgccgt
     5821 tgacgttatc ggttaatacg gtgatgtttt tatccgggct gatgagcacc aggcccctgt
     5881 caacgtcggc ggcaacgata ttgccgcggg cgtcaaaatc aaaaccgaga acgggtccgc
     5941 cggtatcggc atagacctcc tgttgcgtcc cgtcctgatt catgcgcaga atttttccgc
     6001 cggccacggt cgtatacagc ttttggtctt tgccgatgac gatgtgttcg gggccttctt
     6061 cccggcccag tgaaatcata cgcaggccgg caagtccggt gttgacggca tgggggcctg
     6121 aatatccgtc ggacagggga ggaatccact gcacgggatc gaccggaaca ggccagaggg
     6181 ttaaataagc cgtcaaaaga acaacgatga gtgcaaccgc agcaaaaatc tttttcatgg
     6241 cgtcctcctg aagcattcaa aacatcaggg caagaatgac cggctacagc gggatattgt
     6301 tgtgtttttt ggaaagcccc gttacttttt tattccccag cgcatccagc agagcaatga
     6361 ttttttttcg ggtatcgcgc ggcgggatga tttcttctat gatacccatg gatgcggcaa
     6421 aatagggatt gttgaactgc tgttcgtagg cctcaacaag ttgtgcccgc cgggcagtcg
     6481 gattgtcggc cccggcaatg tcgttgcggt aaatgatgtt gcaggctcct tcggcgccca
     6541 tgacggcaat ttcggccgtg ggccaggcca tgacgtaatc ggcgccgagc tgtttgctgc
     6601 acatgccgat gtacgcgccg ccgtaggcct tgcgggtgat gacggtgatc ttcggcacgg
     6661 tggcttccga ataggcatga agcagttttg cgccgtgacg gataatgccc gcccactcct
     6721 gatgggttcc cggcatgtag cccggcacat cggtaaaggt cagcaagggg atgttgaacg
     6781 cgtcgcagaa gcgtatgaag cgcgctgctt tatcggaggc gtccacattc aggacaccgg
     6841 cagcgaaacg gggattgttg gcgatgacgc ccaccggtct gcctgttacc cggataaaag
     6901 cggtgatgat attctgcgcc cagagttcca gcagttcgaa cagctcgccg ttatcggcga
     6961 cggactttat aatttttttc atgtcatagg cccggttgct tttgtcggga atcatcgaat
     7021 ccagttccgg acaggttcgg tccgcagggt ccgtgcaatc ggacagaggc aggggcgcat
     7081 ggcaactgtc gggcagatac gcaagaagct ttttgatttt ttcaatgcag tcggtatcgc
     7141 tttcggtgac gacgtggcag acaccgcttt tggcagcatg ggccagagcg ccgcccagtt
     7201 cgtcatggga aatttcctcg ccgatgaccg ccttgactac atcggggccg gtgatataca
     7261 tgaagctgct ccccttgacc atgaaaaccc agtccgacag agcgggcgaa tagacagcgc
     7321 cgcccgctgt cgggcccata atcgccgtaa tctggggaat ataaccggac gccagggtgt
     7381 tgcggtaaaa aattccgccg tagccttcca gtgaagggac gccttcctga atgcgcgcgc
     7441 cgccggaatc gttgagcgac acgaacggtt tctgcgcggc aagggccatc tccatgacct
     7501 tccagatttt tttcgcatgc atttccccca gcgaacctcc ggaggctgta aaatcctgcg
     7561 cggcaacaaa tacggtgcgg ccgttgactt ttccgaagcc cgtcaccacg ccgtcggcgg
     7621 gaatatcttt tttgtccagg ccgaacagcg tcgatcgatg ttcaacaaaa agctgcactt
     7681 cctgaaaggt gtcctgatca aaaaataaat ccagcctttc ccgggccgtc aattttttca
     7741 gttcatgctg ccgggcgatc tgtttctcgc cgcccatctg tttgagggtc cgattctttt
     7801 tctcaaaatg attgattttt tcctgcgttg aattcttctg tacatcttcg ctcatacaat
     7861 tctccagtct ttgggatccc tacacatgga acatgaagca gtcatgttga aaagatattt
     7921 tatgattgga ggaaatacta gcttttttaa tgaaaactgg caagaagaaa tgatgagcgt
     7981 atccggcgcg gcgtttgtgc aagcatatat tgcctcagac aaaaatctgc actttggagg
     8041 ctcccgttct gtaggaatac gttaccgggt aatccagtct tttgatcagc tctttcatga
     8101 catgattgtt ggaatcaatg agggatacaa aacaggagac gccgtggtcc ctgccgatgg
     8161 caaagatatc cttgagcaga cgcttcccca gccccagtcc ctgccagtcg tcgcgaacca
     8221 gaatggcaaa gtcggtaacc tgttccctga catcgtaacc gtaacgcgca acggcaatga
     8281 tggcatcctt gccgttttcc ggaatgaccg caacgagggc gaaatcactg tcatagttga
     8341 catgggtcag cttgaaaagc aggttctccg ggatcattct gagcggcgtc agaaagcgcc
     8401 ggtagatgga atcgggatac agtttgtcga acagttccag aagcagtttt ccgtcggtgt
     8461 cccggatcgg acggacgaag acgtttttgc cgtttttcag cgccagaagc ttttcgtatt
     8521 gcgcgggata tggtttttta aaactcatca gcatattcta tctatcctac aaaaaatttt
     8581 aggacagccc catgcatgat tctatccggc gacgtctcat ttttttgtcg gagcaacacc
     8641 cggaagcgtt gacggaattt gtccgacaga gcaccgccgg aaaggatatt ctgcccaaga
     8701 tttgattgac agaaaaacag gcttttccta tactcgcaac gttgccgtta gcaggttgaa
     8761 ccgttttgca aaacgcatac cgggattcaa ggcagggtgc tgcgccatgg ttttggccga
     8821 ggcgctgtga tgtcaggcct gcaggtgatt ttgtgatccg tttttagtct ggaggttgtc
     8881 atggaaaaat tattttatcc tgaatccatt gccatcatcg gcttgtcatc gaagccgcaa
     8941 aatattcccc gcctcgttct ggaaaacctg attcgatggg gttacaaggg gcgaatcttc
     9001 gggatcaatc cgcgtgccgc ggattcccat gtggacggca ttaaaatgta caaatccgtc
     9061 caggaattgc ccgtggttcc ggatctggtg tacgtcctca ttcccgcgcg gatgattccg
     9121 gatgtgatgg attcctgcgg ccgggccggc gtgaagcggc tggcgattcc caccggcggt
     9181 ttcaacgaac tgggcgaaga gggcagaaag ttgtccgaac tggttttgca gaaggccaga
     9241 gaatacggga tccggttcgt cggtcccaac ggtgtgacgg tggccaatac cgccaacggg
     9301 ttgtgcctgc cttttgttcc ctcgtacagc ccgcccaagg gaggactctc gatcatttcg
     9361 caaagcggcg gcgtggggct gatgctttgg aatctgatga cggacgaaaa tgtcggcatg
     9421 gcgaaatttg ccagcatcgg caacaaactg gatctggatg aagtggattt tctggaatat
     9481 ttcggacggg atccggaaac caaagtcatc ggcatgtatc tggaaagcgt gccgcgcggc
     9541 gatcgtctga tcgccgccgc atcgaaaatt gacaaaccgg tgattgttct caagtcgaac
     9601 acgacggctg ccggaaggaa agccgccatg agccataccg ccgcactggc caataatgag
     9661 gacattattg atgcagcgtt tgaacgttcc ggtatcatcc ggatccatca gttcaacgat
     9721 tttatttcga tggctaaggt tttcgagctg ccgccgatgc gcggcaagcg catgatggtc
     9781 atgtctcccg cgggcggctt cacggtcatg atggccgatt tgtgcgaggc ctccggtttc
     9841 gaatttgccg atccgggcgc cgatttttat gataagctca aaaattcatc caatgccggc
     9901 gtcatcaatt tttccaatcc cctcgatatg ggggatatct acgacatgaa cgcttatccc
     9961 caaatctttt tctccgtcat gcataatgaa aatgtggacg gcgccgtgtt cgtcacgcag
    10021 tggccggaaa tgccgcgcgg cgaggatgta ttttataaaa tgttccatac ggatttatcc
    10081 aaggaagtgg ccggaacgat tctgtcgtca ggcaagccgt tgggggtttg tctcttcggt
    10141 ctctcggcga ccatttcgcg gatcaaacaa aatctggatt ttccgatttt caacaacgcc
    10201 agcgaaatgg ttcacgcgtt taaaaaacaa tacgactatt acgcgaaaaa agcgcgtggc
    10261 gagaccgggt acccgttgcc tgacggtgtg gacatcgagg cggcgcagaa atggattaaa
    10321 ggacatgccg gggttcgggg ggaagagtcc ctggaactgc tcagccgttt cggcattcac
    10381 gtcgctccat cggatgtggc gaaaaatggg aaagaggtcc tcgctctggc ggatacgatc
    10441 ggttatccgg tggtgttgaa agttgtgtca ccggatgccg ttcataaatc ggaagcgggc
    10501 ggcgttatca cggatgttca aaatgcggaa gaagccgtca aagcattcga tcaaatcaga
    10561 agcaaccttt tccgctacaa cgctcatgcc gattttcagg gcgtgcgggt catgaagcag
    10621 gcaagtgacg gctacgatat gtttatcggc ggaaaattcg atgaatcttt cgggccggtt
    10681 gtctttttcg gttacggcgg catctatatc gaggttttcc aggataccgc caacctgctg
    10741 tgtccggcgg attgtcatga aatcgaagcc ggagtcaaac gattaaaatc ctataagatt
    10801 ttacagggga tgagaggaaa gggagcgggg gatattgcgg gatatgtcgc gatgattgag
    10861 cgggtcagcc atctgctgca tcagttcccg cagattcgtg aactggatat caatccggtg
    10921 cgtgttttag ccgacggatc cggcgttttg gcgctcgacg cgagagtcag aatcgacaca
    10981 tgatgtcagt tgcctgaaaa caatcatcac tcggaatcgc tatgcgcaat tttaaaataa
    11041 cggtggaata cgacggagct gcctactgcg gctggcaaag gcagcataac gggattgcca
    11101 tccagcaact gctcgaagag gcgattaaaa aaatcaccgg tcaaaacgtg tcggtcattg
    11161 gttccgggcg cacggacgcc ggtgtgcatg ccatgaacca ggtcggaagt ttcaaatgtg
    11221 atacgacatt gcccgttcag aagattttta tgggcgtcaa cagtgtgctg cctccggata
    11281 ttgtggtcaa aaatctggaa gaacggtctg acgattttca tgctttgcgg gacgtcaaaa
    11341 gtaaggtata cgtatatcga atttgcaatc agcgtttgag acccgtcctc ggtcgtaatt
    11401 atttctggca tgtgcgttac cctctcgata ttgcgagcat gcggaaggct gtccggctca
    11461 tcatcggaac gcatgatttt tcctgttttt gcgcagcagg gaccgatgtc aaagatcgtg
    11521 tgcggacaat tgtcgatatt gaaattatga ccggagaaga agggctgatt gaaattcaag
    11581 tggagtctca cggatttctg aagtacatgg tgcgaaacat tatcggcacg ctgatagagg
    11641 tcggacgcgg taaacgacaa cctgaggaaa tgaagctaat tattgaatcc aaaaacagaa
    11701 atattgcggg tgcgacagcg ccggcctgcg gcttgttcct gaaagaggtt aaatactgaa
    11761 catgaggata ttgctgctgg gacataaggg catgctggga aacgatctgt tcctgaggct
    11821 ttccgccgag catgaactca tcgggatgga tcaggaagat attgatattg tttctgacaa
    11881 tgactgttgt caggccatca aagatgtatc gccggatatg gtgatcaacg ccgctgccta
    11941 caccaatgtt gacgggtgcg aaacggacag ggaaacatgt tttgccgtca acgccgaagc
    12001 ggtcaaaaat attgccgggg cctgccgtca aaaaaaaatc cgcgtgctgc attttagcac
    12061 ggattatgtt tttgacggaa ccgcccgaaa accgtacagg gaagatgctt tgtgcaatcc
    12121 cgtcaatctc tacggagcat cgaaactggc cggtgagaat tatctgcgga cgttgtcgga
    12181 caattacctt ttgatccgct cctcgtggtt atacggaatc aacgggaaaa attttgttca
    12241 agccattctg gataaggcca aggcaaaaga attcatccgc gataccatga acagggccaa
    12301 agccgttgcc gaagctccct caacgctctc tgtggtcgat gatcaggtcg gttcacccac
    12361 ctatacaaaa gatctggcac tggccgttgc tttcctcatc cggaaaaacg caaccggtat
    12421 cgtccatgtg accaacagag gaagctgcag ctggcatcag tttgccgtca ggattttaaa
    12481 aagcgcggga tatggtcata tcgttgtgga accgataaag tcttccgaac tgcaaagacc
    12541 ggccatgcgc ccggcgtaca gtattttaag caacaagacg tttattgaaa ttacgggcaa
    12601 ggccatgcag ccgtggcaac tcgctttaga ggattacctg aacaatttta aattgaaaaa
    12661 aagtcattcg tgaaatcacg ctccgacgtg ttttctctcc tttggttcat aggtcggaac
    12721 aaaaaagtaa gatcgttttg atgagttgac cgaaacccgg caggtctgtg taaaaatgac
    12781 cgggaaggcg gttggcatcg ttgcaggcga ggctgttcga agatttgccg gaaaggccat
    12841 tgaaccatat ccggttacac aaaagacatc gaacgaaaat catcaaaaag gtctgggccg
    12901 gtgatcgttt agaaaattta ttgtttagat caaaaacagg cttgacgaca gacgggtgcc
    12961 ctttatgaaa aaggcgatgc ttgttcattt aaacgaactg gatttttatt taaagaaaat
    13021 cgatgccgtt tggcctgttc gttgcctcac cggagaggcg atagattgtg aaaaggtcgt
    13081 cgattattac cggcaaagcc ggatcgttta caagtacgtg cattcctaca gcggcgcgat
    13141 gcatatggcc gtcaattccg acggtgtctt caatgcaaag ggtttttaca cgtccctcaa
    13201 cgaaattgtc gagatcatca gtccgttgcc ggccggcgcc gttctggaac tgggatgcgg
    13261 caagggattc aattcggctt atctggcaaa aaaaatacca cacgctcatt ttaccggaat
    13321 cgatgtcagc ggggagcatc tggcgattgc tcgaaaaaaa gcatcccgca tacacaacct
    13381 ctccattctg tacggagact ttcaagatat catgtttccg gacggtgctt ttgatacggt
    13441 ctttgaactg gaatccatct gtcatgcccg aaaagtgcgg caggtattat ccgaagtttt
    13501 ccgggtgctg aaaaaaggag ggcgatttat tgtttacgac ggattccggc aaagcggttt
    13561 ccaccgtttg ccggatcgtt taatcgaggc ggcccggatg actgaaaagg cactggccgt
    13621 cgatcacttc gaagatattg acggctggct ggccatggcg gttgcggaag gattccgcat
    13681 ccatgagaaa aaagatttgt ccgcgtccat catgcccaat cttgcccgac ttcaatatct
    13741 ggcgcggcga tattttgaat acccgatgct gacgaaactg ctgctgcgtc ttttgaatgt
    13801 atataccatg aggaactgca tcgccgttct tctgatgccg tttacccttc acaacaacgc
    13861 gcatggctat tacaaaataa tacttgaaaa atgagtccgt tgtttctatg gtatccaaaa
    13921 cggaaagatt tttactcatt gcccggggga taacgcatgc aagtcaatct cactgaacag
    13981 gaaattgtca aatttgccta tgtcaccaaa gaaatcattg agcagtcgtg gggggcaact
    14041 ctttccaatg cgcagatcaa ttgttatatt gagtgcttct ataccctcaa agatctcctg
    14101 gaacagatag atcgggaata ccttcagaaa cattcggaaa tctccgatta ttttcttacg
    14161 ggaaaaagac tgatcaatga aaaagacaag ctgcaatggg aaaaagaatg gcaggtatag
    14221 cggccccggc aaaaacgatt ctatcgctaa gcacgattcc aggttggaca gcttcttcca
    14281 aaagcatcag cagtgttaaa taacagcaat acgcatttgc aacaagattc gagaaaacat
    14341 gaagattgta ttattgtcca tgccagactc gattccgatg attatgcatg agagtgcatt
    14401 tcacctgccc aattgcggaa ttgccagcat cggcgccaac cttgacgagg gccatgacgt
    14461 ttacgtggtt gatctgatcc ggaaacgcag gcatcttgcg gggtatctga aacgattgct
    14521 tcttaagatc catccgaaac tggttggttt gtcggccatg gcctggcagt atgagacctg
    14581 tattgaaatc atccgcttta tccggcttct gcttccggag gtaaaaatcg ccatcggcgg
    14641 ctatcacgcc acgctgatgt atgaagaaat tgcggcatcg ccggagtccg gtctgatcga
    14701 tttcatgatc cgcggggaag gggaagaggc attccggcgt ctggtcaatg cgctggaagg
    14761 cagcgatcag ttgcaggata ttccctccct gtcttttaaa cgtcggggaa cgttttgcca
    14821 taatccaaga ggagccctgc tggatctttc caggttgaag attcccatac gcgataaacg
    14881 ccgtttgaca tccggctatc acgccgtttt ttccaaaatc gaggttatcg agacctcgcg
    14941 agggtgcacc aggacctgtt ccttttgcag catgcgggag atgtacggac agacgtttcg
    15001 gacgtttccc atcgaaaggg ttacggcgga tatcgatgat atttattaca agcataacac
    15061 ccgcgccatc tttattgtgg atgacaacct ggttttggat cccgaacggg tgatcgatct
    15121 ttgcgatgcg atcattgcaa aaaattacaa gggcttgagc ctgcacgttc aggccgattg
    15181 catttccatt gcggggaacg aaaccatggt caggaaaatg tctcttgcgg gcgtaaaaac
    15241 cgtttttctg gggattgaaa atgtcgtgaa aaaaaatctg atggcgtccg gtaagggcga
    15301 tatcgtggat ctttccagaa aagccgttcg gatatgccat cagttcggca tcatggtgat
    15361 cgccggactg attttcggtt ttcccgatga tgatgaaaac gatatcgttg ataattatcg
    15421 ctttctgaaa tcgcttgacg cggatattcc ctattgccag attctcacgc cctatccgaa
    15481 aacaccggtg cgccagcagt tgctggaaag aggcatggtg acgaacctgc atgattacaa
    15541 actgtacaac ggcctcagag ccaatattaa aacgcggcat cttgatactc aacaactgca
    15601 gtatctggtc tggctgcatc ggcagaagat gttcggctgg tgggaaccat cccgtctcat
    15661 gagaatcaaa gggggtctct gggtaacgtt ttggacacac ctgttgcgtc ctttcctcag
    15721 gagcacggtc ggtcggcaac aggcgcaaaa atcatggaag gaactttacg aaagggatgt
    15781 gaaatatctg gacggtttga accagttcaa gaacctggcc cgaaattact gaagggccgc
    15841 atccggtcgt tttcgccggt gagaaacgtt acaaagaata aagggggaag atcgtgaaac
    15901 tggtcattcg cggcggaagc atcgccgccg gctacggcgt gacgaaaagc tatgttgata
    15961 ttttaaccca agcgctgctt ccggaagggg tgtgtgtgat caaccgcgcc cgataccggg
    16021 aaacctcttt tgacggcatc gagacctatc cgcaggatat tgaccggtac gagccggatt
    16081 ttcttctgat tcacttcggc gtggatgatg cctttcagtg cgtttatcgt tcagaatttc
    16141 aggaaaatat cgtgcagatg attcgtctga gccgcaaacg attgcagccg gttatttttc
    16201 tggcgacatc gcatacgttt gataatccca atgacatgga tgccgttcac atcttttacc
    16261 ggtcgctgcg gattgttgct tcagatctga agtgtgaatt catccccgta cacagaagct
    16321 ggaacgctta ccttgaggaa cacaatcttt gcagccagga ccttacgcag gaggattcgc
    16381 gctatccgaa tgaacaaggc catcaggtta tcgctaatgc catgatgaac gtcctgggta
    16441 aattctttta aaaattcatc cccggtgccc caggaattca tcactttgcc cgggcgggaa
    16501 atcttttctg cagggagtca taatggtagc cggaaactgc caaggcccgc accgcctgct
    16561 cggcggtttc aaagatcggg atgcggtgct tggcgcggat gccgatggac ttggctatgg
    16621 cgttgggcgt atagatggcg gcgggtttct gataatgcct gcacatcttc atgagctcca
    16681 gaagggtatc gattacaaac gtctgccaga cgctcaacag cggcaggagg ccgtccactt
    16741 catcggcgga aagaaggatt tcataaatat cgagataggt ctgcggcaag gcaggaccga
    16801 tatcgaccgg attcttgata ttgaaaacct gccccgcctt ttttaatttt tcctgcgttt
    16861 ccggtttcag gcgcacgacc tgaagatgcg catccaccag cagatccgct ccgatgctgc
    16921 caaacgctcc ggaatttgtg aacatggcaa tatttttgcc tttcagtagg ggcatctccg
    16981 tcagcatttt aggcatggaa tgcagttcgt cgatggattg cagacggatc attcccgact
    17041 gtttgcaggc gctgttgaaa atgctgtcgt tgttggccat gccggccgtg tgagacatgg
    17101 ccgccagggc gccttcctgt gtgcgtccta ctttgtaaat caggatcggt ttacgcgatt
    17161 ttctggcagc ctccatcagc ttgcgaccat ttgtgacgct ttccagatac agcccgatca
    17221 cctgcgtttc atccgtctga aaataatcga tcagatccgc ttcatccaca tcgcatttgt
    17281 tgccgatact gacaaccttg ttcacgccga cgagatcgta aaaaaaggac tcaatcacca
    17341 gcacgacaac gccgccgctc tggatgataa aggaaatgtt acccggtgct tcgaatatcc
    17401 ggcccatttc gaccaggctg ggattgacgc catagaaaca gcagaatttc ttgcgggtat
    17461 ccagtgttcc cagacagttg ggtcccatga tccgaatgcc gtgggctttg gcgatttcgt
    17521 cgattttgcg ctgcatggcc ttgccgtcac tgccgccttc ggaaaatccg gcgctttcga
    17581 tgatgacccg cttgattcct ttccgggcgc aatcgccgat gatcctgggg acgtttttgg
    17641 ccgccacgat aatgacggcc agatccggac tttcgggaag atcgagtatc gatgaataac
    17701 ccggatagcc gttcaccttt tcgcctttcg aattgaccgc gtaaactgca ccgggatatt
    17761 gaagataatc cttcagcatg ttacaaattg tagctccaag gttaaaaggg gtattggagg
    17821 cgccgacaac aacaacagaa tggggatcga aaaatttttc catatgcaat tcacctgcag
    17881 caagattaag cttttttttc tattcaaatc catcacgctt tgtcaagaaa gaaaaatgtt
    17941 caaaccgccg atgactgtcc ggtaatcatg gttgcggatt ttgtttttat gtaatattag
    18001 tataaccgag tttatcagga gaaataaata ttgtccaagc gcatcattgt tctgtccgaa
    18061 gacatcgcca ataagattgc cgccggagaa gtcgtagagc ggccggcttc catcgtcaag
    18121 gaattgctgg aaaacgccat tgacgccggg gcgaccgata tccgggttgt attggaaaag
    18181 ggcggtacgc agtccatcag ggtcaccgac aacggctccg gcatcgatca cgacgatgtc
    18241 ccgctggttt ttgaaagaca tgccaccagc aagattcatc aatttgaaga catattcaat
    18301 gttgtgtcgt tcggttttcg cggcgaagcg atgccgagca ttgcctccgt cgcgagagta
    18361 gaaatgttaa ccagacggaa aaatgatgtg tccggcaccc gggcgattct ggacggcggt
    18421 gtgatccggg ccatggatcc ggccggaacg cccgaaggca cgcagatatc ggtcagcggc
    18481 atctttgccc atgttcccgc ccgccgtaaa tttttaaaaa cggaagcgac ggaacagggg
    18541 gcctgtctgg atgtgattac ccgtctggcc ctggcccatc cggaaatccg tttcaaagtc
    18601 gaggcaaacg ggaaggaaat attcgcggct ccggaagtcc atgacacggc caagcgcatt
    18661 gccatggtca tgggcggcga gttttcggcg cattgcatgg cagttgatgc acagcaggac
    18721 gccatgaccc tgaagggatt tatttcgcgt ccggaatact ccaaatcgaa ttccaaaagc
    18781 atttatctgt ttgtgaataa aagattcata cgggatacca gcctgacgca tgccgtattg
    18841 tcggcatacc ggcagattat tgagtccaga cgttatcccg ccgcggcgct gtttctggag
    18901 gtgccgcccg aagacgtgga tgtcaacgtc catcctgcga agatggaggt gcgctttaaa
    18961 aatgcgcatg gcgtttatga actggtctcg aaaaccattg ctcatcattt gtcccgtgcc
    19021 gaaacatcca gaggacatta cctgtatcgt ctggcgcctg cggaaaaaaa agattatttc
    19081 catcggcagc cttttgtgaa tgagccgtct ccgtccatgc cttccagacc cgctgaccgg
    19141 tcgcatgcac aacagggaat tcaaaacatt ttattgcacc gctcacaggt gtcgcaaccg
    19201 ctggctgctt cagaagggga tgcgggtgtc cctgataaaa caattttatt tggcgacatg
    19261 agatatgtgg gtcagtacga cggcacctat cttctttttg agggaccgga tagcctcatt
    19321 ctgcttgatc agcatgcagc gcatgaacgc attattctgg agaaactaaa gaaagactcc
    19381 ggtaaaaagg cggccggtca gtcgctgctg ctgccggaaa ttgtcaacct gacaccggga
    19441 caaatggcct tgctcaacga tgctcttgat tttctcgaaa cgatcggtct ggaaatcgaa
    19501 tgcttcggcc gggacgcggt tgccgtaaag acggtgccga caatgttatc caaagtgaag
    19561 atcagcgaaa tgatatacga tatcgttgat cagcttgccg accagaacag aacgccttct
    19621 ctgcaggaac gcagcgacaa ccttctggcc tccctggcct gtaaagcggc gatcaaagcc
    19681 aaccgattgc tttcccggga cgaagtggac gcgctgtgcc gcgaacttgc ggaaacgccg
    19741 tttcatgcaa cctgtccgca cggacgtccg atcaccattc agttctcctt aagcgaaatc
    19801 gaaagactgt tcaaaaggaa gtaaaaaaac cgaagatgaa gaaactgccg ctggttatca
    19861 tcaactcacc gaccgccacg gggaaaacgg atctggccgt gtatctggct ttggaatgcg
    19921 gcggtgagat tatcagcgcg gattccatgc aggtctaccg ttatctcgat atcggtacgg
    19981 ccaagccgac gatggaacag cgccgtggga tcaggcatca tctgattgat gtcgtgaatc
    20041 ccgacgggga attcaacgcc gctttatttg ccgcacgcgc ccgggcggtc attgaggaac
    20101 tggttcatca ggggaagccg gtcttcgttg tcggaggaac ggggctttat atccgggcat
    20161 tgctgaaggg aattatcgac accccggatg tcgatgcatc catcagaaat cattatcgga
    20221 gcctccggga aacgcacggc aaaatctatc tttatgattt gctccggcaa aaagattcca
    20281 cggctgccgc gcaaatcaat ccgaacgatt cggtccgcgt gatccgggcc ctggaggtgc
    20341 tggaacagac cggcgaatcg attacagtga aacaaaaaaa acattctttc agcgaagacc
    20401 gttacacaac ctgcaagatt ggactcaggg tggaacgcgg cgaattgaag gaaaggattg
    20461 aaaagcggac agatgcaatg atccgggccg gcctgctgga tgaggtcaaa ggcgttctgg
    20521 caagaggata cagagaaaat ctgaaatcgc tgcaatcgct gggctataaa cagatcatcg
    20581 gattcctcaa ctggcaatat gactggcgcc actgtgtgaa tttcattaaa cgcgacacgt
    20641 ggcaatacgc ccggcgtcag atgacctggt tttccgccga taaggaaatc aactggtttg
    20701 cgcccgatgc actggacatt ctgagaagac atgttaaaat atttcaaaat gaaaatcagg
    20761 agactgaatg acccgaagag atcaccatac gtccgttgag tatttgtgcc ggcttcagag
    20821 gctttttgtc gtcgtcctga ttgccctgcc gttgaatatt ggcgcggcaa ctcattacca
    20881 ctgtgcggac cgaagcggcc ttcagacgtt ttccgattac cctctggagg atcaggtttg
    20941 caccgaaatg gtatcgcagg aggagacgga gattccaaag acggatagac ccattgcgcc
    21001 caatgcggaa tcgcccgaga aaaaaacaac cagaatcgcc gtcaaagaaa acggtatttt
    21061 ggttcctgta acgctcgggt atgacggccg ggaggcagac atccgtttgg ttctggatac
    21121 cggtgcttcc gccacgacca tctacgacaa tgtagccggc cgattgctgg taaatttaaa
    21181 taatgcgcca aaggcgcagg ggcgggttgt cggcggtgga accattgatg ccaatcgtgt
    21241 gacgttcagt tatctcagga tcggccccaa tacctttcgc gactggaata tttttgttgt
    21301 ggaaaataag gacggtgata cgtcatacga cggtctgctc ggcatggatg tcttaagcac
    21361 catgcggtat aaaatcgatt tcaggaatca gatcattatc tgggaatgag tttttataat
    21421 aatgtgtcta ttgtgcaatc tttcaccgtt ttatccgggg aacgatgatt cgctcacagt
    21481 ctccgtctcg tcattcatgc cggagggaca ataacggaca atgcaatatt gcttgactta
    21541 aattggttct aaagataata atatcaatct gtttggacta tgtggcgatg atgaaccgaa
    21601 aaagagacag aataatattt ttcaccctgt tgatttttgc gtccctgctg acgttggccg
    21661 gctgcgaacc gaaagtacgg gttgcggtaa gtcaactgga tacgccgggg caccatacct
    21721 acgccggcct gaagcttctg gaactcgaga agtattccga tgcgattaga gcctttaaaa
    21781 tggcgaccca gctggacagc aactattcca aggcttatac ggggatggcg ctcgtcggca
    21841 tagccacagg caaactctcg gcggcggcgg atgatcttga aaaaggaatt ctgaacgcca
    21901 aaacatccga tgaagaactt tttgtcaaca cggcggctat tcgttatcac acggcaaaaa
    21961 aatccgatga aaaatggctg gagaaggcaa agtattattt tgtcgagacc gtgatgatcg
    22021 attctcaata cgcgcctgtt tactatttca tgggcattgc ctatcaggaa gcgttggaat
    22081 tcgaccaggc ggcaaaaatg ttcaacaaag tcattcagct gaaagccggt catatcgagg
    22141 atgccaataa ccggttggag ttcctggagc tggtgcacaa agcccacccc gccacgcaag
    22201 ccgggaagca aatcgcgctc aaggaaacgg tgagccgggc agatatcgcc atgatgctgg
    22261 ccgaggagct aaaaataaaa gagctctatc aaaaatattt gccgaaaacg gcacaggagg
    22321 cgcaaaccga agaggaaaaa gttgccgcgt cgatgagcca ggctgaacac gttgcaaaag
    22381 aaatggaaaa aatggatcag tttcattcct acatggtttt gcaggagaaa gaaccggtga
    22441 aactgctggc caatgacatt gcccttcatc ctttgaaaaa ttgcattgaa gccgtcctcg
    22501 aagcaaaagt tcacggtctc gaaaatgatc ccaaaggcaa ttttaaaccg gatgaaattc
    22561 tctcccgcgg cgaattcgca attattctcg aagatattct gataaaagta tcgggagaga
    22621 gagatatcgc cgccagatac gtcagctcca attcgttatt tccggatgtt cccgcagaca
    22681 tgccttattt tacgccgatt atttcagtga catcacgggg aatcatggag gcaaaaaata
    22741 caaagacggg cgagttttcc cccttaaaac ccatgtccgg tgttgaagcg cttctggcgg
    22801 ttaaaaagct taaggagaat ttaaagatcg attaataagt tattaaaata taaaatataa
    22861 ataataaagg ggcgagaagc ccttttcgtt tgtagacccg aatgaggagt aatgatcgca
    22921 tggcaaaaag agaattgggc aaggcttttg aaccggccgc agccgaaaaa agattatatg
    22981 aatactggtt gaaaaataaa tattttcacg ctcaggataa aagcgaggcc gaggcgtttt
    23041 ccatcgtcat tccgccgccg aacgtgacgg gtatgctgca catggggcat gcgttgaata
    23101 ataccttgca ggacatcatt atccgtttca agagaatgca ggggttcaac acgctgtgga
    23161 tgcccggtac ggatcacgca ggcattgcca cccagaatgt ggtggaacag cagttgctca
    23221 aggaaggaac atcgcgtcac gacctgggac gtgagaaatt tatcgagcgg gtatgggaat
    23281 ggcgccggaa atacggcggg gtaattatca atcagttgaa acgtctggga tgttcctgcg
    23341 actgggaacg ggagcgcttt acgatggatg aaggattgtc ccatgccgtt cgcgaggtat
    23401 tcgttcgtct cttcaatgaa ggcatgattt atcagggcga ttatatcgtg aactggtgtc
    23461 cgcgctgcca caccgccatt tccgatctgg aagtggaatt caaatctgat gcaagcttcc
    23521 tgtggaacat ccgttatccc ttcgccgagg ggcaaggcga aattgtggtg gcaacgaccc
    23581 ggccggaaac catgctcggc gataccgcgg tggctgttca cccggacgac aaacgataca
    23641 aaaacctaat tggcaaatac gtcatactgc ccttgatgaa taagaaaata ccggttatcg
    23701 ccgacgatta tgtgacgatg gatttcggat cgggggccgt aaaaatcacg ccggcatcgg
    23761 atcccaatga ttttgccatg gctcagcgac atcatctgga gatcatttcc gtcattgacg
    23821 gcaaggggat catcaatgaa aacggcggag cctataaagg tcaggatcgt tatgaagccc
    23881 gcaaaaacat tgtcaacgat cttcaaaagg gcggatatct ggtcggtacc gagccgtacg
    23941 ctcataatgt cggacaatgc taccgctgta aaaccgacat cgaaccgatg gtctccaagc
    24001 agtggtttgt aaaaatcaag cctctggcca aacaggccac cgccgcggtg atcaagggga
    24061 aaacgaaaat cgttcccaaa acatgggaag caacgtattt tgactggatg aacaacatcc
    24121 gcgactggtg catttcccgc cagctctggt gggggcaccg cattccggtc tggtattgcg
    24181 acaactgtaa aaaagtgatt gtgtccacgg tggatcccga cacctgtccc gattgcggcg
    24241 gcgcctctct gaagcaggac gaagatgtcc tggatacctg gttcagttcc gcgctctggc
    24301 ctttttccac gctgggctgg ccggaaaaaa cggctcctct gaaaacgttt tacccgacct
    24361 cgttgttaat caccggattc gacattctct tcttctgggt tgcacgcatg atgatgatgg
    24421 gcctttacgt gatgaaggac gttcccttca agcatgtgta cctgcatgcg ctggtcaggg
    24481 atgaaaaagg cgagaagatg agcaaatcca aaggcaacat cattgatccg ctcttaatga
    24541 ttgatcaata cggaacggac gcgtttcgtt tcaccctggc cgcctatacc gctcagggac
    24601 gcgatgtgcg cctttccccc gaacgcatcg agggctataa attctttgtt aataaaatct
    24661 ggaacgcctc caaattgaca tttgccaatc tggacgattt tgacgaaacc gcaactgcgg
    24721 aggttgccga ttcgctggcg gatttgtgga tcaaggcccg tctgaataaa gcggtggagg
    24781 acgtgacgac cgccctggat gaataccgct ttaacgacgc cgccgccgcg gtttaccgtt
    24841 ttatctggca tgaactgtgc gactggtatc tggaactgag caaaccggtt ttctacggca
    24901 agggaagcgc agaacaaaaa caggccgccc agaaaacgct cggtgttgtt tttccgacta
    24961 tgctgaaatt gttgcatccc ttcatgccgt ttttgacgga agagctctgg caggtctttc
    25021 acggggacga taaaaattca atcatgatca gccggtttcc ggcggtttgt tcggaatgga
    25081 aaaacgacgc ggcggaaaaa gaaatggaaa cgatcatgga tatgatcacc gccgtccgga
    25141 atatccgggg tgaaatgcgc attgcgccgt cgcaaaagct cgatgttttg atgtccaccg
    25201 tcgatgaaaa aacccaaaaa atggtggaga aacataaaga ctatatttta aatctggcca
    25261 atctggaatc cttaaccgtt gaaatcaatg cggtggaacc aaaaggtgtg gccacggcgg
    25321 ttgtcggctc gacaaaacta ttcgttccga ttgtcggcat cgttgatctt tccggcgaaa
    25381 tagcccgtct ggaaaaggaa ctggccaaga tatcaaaaga tctgcagcag agttcgaaaa
    25441 agctggccaa tcgcgatttt atgtcaaaag ccgcgccgga catcattcag aaagaggaag
    25501 acaaggtgaa ggatttccag aaaaaacaca ccactctgga aaacgctctt aagaaattga
    25561 aaggcatgtc cgcctgatgg aacagaggat tgtgagaatc attcaaaatg cattggccga
    25621 ggatatcgga tccggcgata ttacgacagg cgcgacggtc aatgcccgaa aaaagggcag
    25681 ggcggtggcc attgccaagg ggaattttat tgttgccgga acggacgtat ttgccgggac
    25741 attcaggacg ctggataaaa acatcaaggt ccaaaaaagg attccggaag gatatccggt
    25801 aaagaaaggc gacatcatcg ccgaagtatc cggccgtttg gaccatattc taatggcgga
    25861 acgcgttgct ctgaatctgt ttcagcggat gtgcggcatt gccacgctga cccaccggta
    25921 tgtcagggcg gttcgtaaaa cgaaagccgg aattctcgac acacgcaaaa ccgttcccgg
    25981 attgcgcgtt ttggataaaa tggcggtcaa aaccggcgga ggacaaaatc accggttcgg
    26041 tctttacgac ggtgtgctca tcaaagataa tcacatcgag gctgcaggcg gaatcacagc
    26101 ggcggtaaac gcgcaaagaa aaaaactttc acgtttcatg aaaatcgaag tagaaacccg
    26161 gaatctggat gaagtccggg aggcccttga gtgcggcgcc gatatcatta tgctggataa
    26221 tatgagcgtt tcggccatga aaagagccgt atcgtttgta gccgggcgcg cgcttctgga
    26281 agcatccggc aatgtcggcc tgaagaatgt gatggatatt gcagccaccg gcgtagattt
    26341 catctcggtt ggggaactta cccattccgt gcaggcggcc gacatttcac tcaaaatcag
    26401 gtcttcatcc tgatggaatg ctcttgatgt atggattgga acattgcccg attgcaggaa
    26461 accctcggca caggactatt cggcggccat cttcattttt atcctgaaat cgtatcgaca
    26521 aacgacgaag ccatcgcgct ggcgatggcg ggtgcgccgg aaggcactgc cgttctggcg
    26581 gatgcgcaga ctgccggaag ggggcgctgg cggcgctcct ggatttcacc gcccggggtg
    26641 aatatttata caaccatcgt tttaaggccg gaatgcgaat cttccggggc gtctcagatt
    26701 cctatcatgg caggcgtagc cgcagcagaa gttctggacg gcgtttgtcc cggccgagta
    26761 aagctcaagt ggcccaatga tgtcataatc ggccccaaaa aagtctgcgg catcctgtcc
    26821 caggcaaaaa taacatcaaa aaaaattgat tttgccgttg tcggtatcgg cattaatgtc
    26881 aacgcgggtg tgcaccattt tccaaagacc attcgggagt cggcaacgtc tctgttgatc
    26941 gagtcgggcg gagaaatttc ccgtcacgat ttgttaatta gcctctatga aaatcttgaa
    27001 aaatggtata aacagctact gcaaaaagga tttgagcccg tccgaaaaaa gtggctcgaa
    27061 tcggcggcga tgatgggacg gcccatccgg atcgcgttta agggtgaaag cacagaaggc
    27121 atagccaaag ggctgggtga tgacggttgc ctgattcttg tcgttgaggg caatgaagaa
    27181 attaaagtct ctgcgggaga cgcaacgatt atgaggtgga aagaaaatgc ttttggtgat
    27241 tgatgtcgga aataccaaca ccgtactggg gctttttgac ggaaaaaatc tgatccatga
    27301 ctggcgcata cggacggaaa tcgaccatac cattgatgaa tatggtgtgc tgatttataa
    27361 cctgtatcag tccagccgga aaaaagaaaa agccttcaaa acggtttccg ccattattat
    27421 ctcatgcgtg gttcctccca tgctcaatat tctggaaccg ctctgtatca agtatttcaa
    27481 tgtcaagccg ttgatcgtcg gaccaggcat taaaaccggg atgccgatct tctacgataa
    27541 tcccaaagaa gtcggcgccg acagaatcgt caatgccgtg gccgcgtaca ataaataccg
    27601 gaaggaagcc attatcgtcg atttcggcac ggccacaacg tttgattatg tttcccctaa
    27661 gggggaatat atgggggggt gcattgctcc ggggattgtc atttccagcg aagcgctttt
    27721 tgaaaaagcg tcaaaactgc cgcgtgtgga attcagccgg cccaaatccg cgattaccaa
    27781 agatacggtc agcgctattc aggcgggaat tatgttcggt tacgccggac ttgtcgacgg
    27841 aatcgtcaac aggatcagga cggaagtcga gacaaaaccg ctggtcatcg ccacgggagg
    27901 cctggccaat attatcgcac ccgagactaa aaccattgaa aaaatagaag agatgctcac
    27961 cctcgacggt ttgcgtttta tctacgaact gaataaaaaa taattatgct caatgtcgga
    28021 ctgacaggtg gtatcgcctg cggaaaatca accgtcgcga aaatgtttgt tgaaaaggga
    28081 gcgcatctca tagacttcga caagctggcc catcggctgc aggaaccggg acagcccgcg
    28141 tggcagaaag tcgttgatta tttcggcagg caaattctcc gtccggatca aaaaatagac
    28201 cgcgtcaaac tgggccgcat tgtctttgcc gacaaaaaaa aactccgggt tttaaacaag
    28261 ctcgttcatc caccggttta cagggaatgg cagttgcgcc tggaaaaaat cggcaggaaa
    28321 gaaaaacatg ccattgtttt gtcggatgtg ccgctattat tcgaggggaa aaaaactcat
    28381 cttttcgatc tcactatcct ggttttgtcc gatccggagg atcagatctg ccgtttgatt
    28441 tcccgtaatc gcatatccgg agaagacgcc cgggcacgat taaaaagtca gatgccgatc
    28501 ggcaaaaaaa cggcgttggc ggatattgtg atcgacaaca gggacagcat tgcgcgcaca
    28561 aaaaaaacag tggatcgggt ctggagaaag cttgtccgac tggaaaaaac caaatcggcc
    28621 tgatagacac aggccgtcat tattcgagaa caaatcaggg aggaaacaat gatcgaaaaa
    28681 aacgtaaaaa cagatttcag ttcaaaaata tccgaaccgg tcatcggtgc gggtacctat
    28741 gtccatccgc tggccgcggt tatcggcaat gtcattctgg gtgaaaatat catggttgcc
    28801 ccgacagcct gcatacgcgg cgacgaagga cagccgctgc atgtcggcga tgattccaat
    28861 gtgcaggacg gcgtgatcat tcatgctctg gaaacagaac tggacggcaa accggttgaa
    28921 aaaaaccaga gggacgttga cggcaaaaaa tatgccgttt atgtggggaa gcgtgtttcc
    28981 ctggcgcatc aagtgcagat tcacgggccg gcggttgtca tggacgatac ctttgtcggt
    29041 atgaaaacgc tgatttttaa atcttttgtg gggaagcatt gcgttattga accgggtgtg
    29101 acgttaatgg gcgtcacggt ggacgacggc cgttatgtgc cggcgggatc tgtggtcaaa
    29161 acgcaggccg acgcggatgt cctgccggtc attacgccgg attatccgat gaaagaaatg
    29221 aataaaggtg ttttgcatgt gaacaaggct ctggccaagg ggtatctggc agcggctaaa
    29281 aaataacgtc gtaaaaggca tccggtattt tcaacggccg gatgccttgc agaaatgaaa
    29341 tgttgacaca cgcttcggaa attaaccatc atcatacttg tttgtcctgc ggaaaaacag
    29401 acccggtcag caaacgacgg tattgttcgg tgaaatgccg acaaaatctg cggcagaaac
    29461 tcaacctgaa gaacggattg ctgcaggcct tgaatacgaa atacgcaacc ttctattttt
    29521 cgaagaccat gatcattctg gatgtcgtgc cctacgggtc cagggagata ttccggtatg
    29581 ccatcaggcg cagagcggga aacacgcctg ccatcgattt cagttttatg accaacaatc
    29641 tgggaaaggc ctggtgggct gtgtcggaca caacgcacag aaattatctt gcgtcgcagc
    29701 atgtgttaaa aatggcgaag cgttatacga ttacggacaa atcgcagcgg ccgaaaatag
    29761 ttaaaatacc ggcggtgaag gtgtcagcgc tgaattgtct ggacataaaa aaaatcgatc
    29821 tcgcatcctg tgacctcgac agggtcatca aaagcgccta tcgccgtcag gtgaaaatct
    29881 atcatcccga tctgggcggc aaagcggcag atttcaggaa gattcacagc gcctacaaag
    29941 agttgctggc gtgggcggat catcctgtct ttgtccatcg gcgggggttt cccgataaat
    30001 ggttttacag cgcagaaacc caaaaatggg ttcagccgac gccgctatcc gactgatccg
    30061 gccggattat ttccgaaaag actgcaaatc ttgaggggtg ttgatgttcg tgaagagggg
    30121 tgtatttccg gcaaagggtc ggatgtcttc ttcggtgagt ctgagcgtat gcatgtcttt
    30181 gaataatccg gcgattttca atcggtcggc aaggatcatc tttttcataa cggccagaca
    30241 gcggcgcgaa tagatggcat gcagggcctg gaatccttcg gataactcgg ggacgatgat
    30301 gtcgtatgat cgccggcgtc cgatcatgta acggatgaaa tcttcattca ggcagggcat
    30361 gtcgcaggcg gcaacaaaac agtcatcgct tgcggcgtaa aataatcccg tataaattcc
    30421 gccgagggat cccttgcctt tgtagatgtc cgtgacaatc atcgcctcgg aaaattccac
    30481 gtacgccagc ggatcattgg ttacgataat gatctttggg aaaattctgc gataaacgga
    30541 cagggtcctg tccatcaggc gcttcccgtc tatttccaga aacgccttgt tgacgcccat
    30601 gcgtgaattt ttcccgccgg ccaaaataag tcctgtgacg ttttcctgca tatgatcaat
    30661 tcctaaaggg ttgtccgatg gtccgtctcg tcctgattcc ggaatcgcat cacgttcagc
    30721 atgaatatct ccagtttgct gatcacattg ggcggaaaaa gattcccgtc gatttcaatg
    30781 gagatggccg gatattttct ttccctggcc cagggggtca ggacgccctc gatcacccgg
    30841 ccgatcaggc aggcaaacgg ggagatgttg acgatgccgg cataaccgtc catcatggcg
    30901 gtggctgcga caccgctgga gatgctgatt tcggaataca gttcgttgtt gacgaaatgc
    30961 ttttcagtgt tttccataat ttcatccatg ttgtgcggag cgtgcggaac caggccggtt
    31021 gtcgaaagaa tgtcgcggat gtttttctcc actttctttt tatagacgtg ctcgattttc
    31081 catttgacga tatcggaaaa ttccgcagcc agaaactttt tgtaccaggg aacgagtttg
    31141 agacgttttt gcagttcgtg acggcggacg aaatcacaat agtaaaccca ctcggcaaca
    31201 ttggagacct tgccgataat accgcgccgg ctgaaatgcc tgatcagttc atctactgca
    31261 aaatcatcgc gcctgacgta aatctctccg acaatcaata ctttcgggca gtcatcgatt
    31321 tttttcttta aggggatttt ggcgatcgaa gaggatatct ccttcagaac gggaagcgat
    31381 tgcgtgacat ctttttccgc aacgtcgatc agtttttgcc agatgatatt gtaatcggaa
    31441 acggccttga ccggatccgc cgcacaggtg cgcaacgaag tttcaatatc cttcatataa
    31501 tcgccgatga gtacggccca ccaggcatat ttggaaaatt ccgttcccag ctcgttataa
    31561 gaattgtcgg cgtccagcgt gaaaaccacg acattttcca gacgcagatc tttgaaaaga
    31621 ttttcataga aaacaaaata ctggccggtt cggcagggac cggttgtggt tggcacgaac
    31681 agaatgtaaa tttcgtcttt gcggtatttt tccgacgata gatatttcaa cgtactgccc
    31741 agaacgagat gagagggcag gcattccttg ccggaggcgt gattgcgtgc catttgcagc
    31801 gtatagatat ccggcacggg catggtttcc gcatgaatac ccagcccgcg gatcgtggcg
    31861 gccagcactt ccaccgacag tcttcccatg ttggataaaa gcagcttgac gcggggattg
    31921 tttttgatgg gaatcttttc gccggtgagg gtgtttcgga tatgaatgtc ttcgccggaa
    31981 gtgttgataa accttagacc gttatcatac cggctttctc tgatgtcgct gattttggaa
    32041 cggtaacctt cgatgatgtc cagaaaggcc tcgatacgcg tatccacgcc ggcatcggcc
    32101 gtgtgggaat ccagctccag aaccagaaag ggtttcatcc ccattatcca cttcagatag
    32161 tgaagcatga aggaatcggg agcgcaggaa aaattggaga tgtatgttac aaagatattg
    32221 tcttcgtttt tcaaaagcgc ggcagctttc atgtcctgct gaccgtagta ccagtacata
    32281 ttgggataaa tcccctgatc ctcaaatggc aggatgtcaa aaggaacgac ggtgaagccg
    32341 cgggaggtgt attttcgcgg aatacccatg ttcgcatcgg gtgtaaacgc gttgtagggc
    32401 cgccccagaa cggcaataac gggtctttgg gatttgcggg aattttcaat tgctatttta
    32461 cccagttcgg cgaatttcct aaagcattcc atttgctttt ccagggcaac ggaaaaagcc
    32521 aggcgggcgt cctcctgatc aaaaccgagc cggacagcca tggcgataaa cgattcggag
    32581 gctttctgtg tcccgtacat aaaactgaca acgggagcga ggtatttccc ctccggaatt
    32641 tctggaaagg cttttttaat atagtaaggc aaactctgcg tgatcggaca gaaacaggcc
    32701 ggtgtattgc tttcatagct ttccatatcg cggaaatgcg gcagaaaaat ataatccacg
    32761 ttgcggttga aaatatcctg aacggcaccg tgggcaattt ccgccggaaa acagtacgtg
    32821 ctttccaccc gggcaacgcc ctcgtgtgtg atgtcgctgg acaggacggt ttccacaccg
    32881 agcgtatgga aaaaccaagc gtaaagcggc cagtgcgaat agacggaaaa gcatttcgga
    32941 atgccgacga cgaaatcctt ttttctgatc aggtcggccg gtcggggagc gcattgatta
    33001 aagagtaggt cgttgcgctg ttcgatataa tcgacaacat cgctttcatc gaaacttttt
    33061 ttcttcctga tgttggcgta tttgttacaa cggccgccga acatatattt gcggccgttg
    33121 acattcagta cgcgtatcgg gcagaagtta tcgcaggcgc tgcactgaaa ttccttttca
    33181 tggagaatct cggtcgacag aatcttttca atatcgtaat ctcttctgct gagaaatccc
    33241 tcatgcagtt tctgtcgggc aagaatgccg acgccgaagc aacccatcag ttccggatcg
    33301 ggaggcacca gaatattctt gtccagaagc atggcgaaag ccagaggaac cgccttgttc
    33361 ttggcgacgc ctccctgcag gacgatgttt tggccgacgg ttctgttgcc cacaacgcgg
    33421 ttgagataat tggatacgat ggaggtaacg atacccgccg taatgtcttc acgcgacgcg
    33481 ccctgctgaa tggctttccg gacatccgaa ttgatgaaag cggaacagtg ctccccgaac
    33541 ttcaggggtt ccgacgcgcc aagcgcgata tcgccgattt cccaggcatg atgaatgttc
    33601 agatcgccct gcgcggattc ttcaagaaag gaaccggttc cggcggaaca ggcttcattc
    33661 attgcatagt caatcggcac cttgtttttc agataaacgt acttggcatc ctgtccgccg
    33721 atttcaaaga tggtgtcgat gtcttcctga taaaacgtcg ttcccaccgc atgagcaatg
    33781 atttcattat acacgccggg cgtttccaga aaaacgccca gcagttctct ggaggatccg
    33841 gtggtggcgg ccagggttat tttaattgct cggtcgccga tatcctcccg gatctgcttt
    33901 tgcatctcca agaagcaatt tttcagggct ttgaccggat ctccatgcgt gcgtccgtaa
    33961 aaggaggccg taatctcgga ggtttccata tcaataagac aggctttggt ggtggttgac
    34021 cctccgtcga ccccgagaat atattccctt cccgcaatga cttttgattt tttggatgga
    34081 agataatgaa ctttcccctc ggccgttttc aagcttttga atctcttgaa ctggaccata
    34141 tgggttttga aaagatcgtt caaggcgggc aggggagtgc ccatcgagcc ggccagcagg
    34201 gcggcgccgt aggcttcaaa gtaaacagcc tgatcgggga cgataaaatc attttccggc
    34261 atgcgttggc ggatgaagcg gatcagatgc gggttcctgg tgacgccccc gaccaacaga
    34321 acttttttgc cggctatttt tgctttttta atgaaatcga tgaccttggt tgccatgaca
    34381 tcgctcaacg aaagcacgat gtcgcccgtg ctggcctccc ccttgttcag acggtgggta
    34441 cagtcgcttt tcatgaagac agaacagcgg gaggacagtt tcagaatccg gctttcttca
    34501 ggaatggaat gaatatcgtt gagatgcatg ttcatccggc cgagctgctg tttgaaaaac
    34561 tcgcccgtgc ccgaagcgca tttgtttccg gaaaagcttg tgatgatttg ctgattttca
    34621 tcaatgctgt aaacgacaag atcttcgccg cccagagaaa ccagcgcgtt aacgttgccg
    34681 tttaaatgcc ggagcgcttc ttcaatgcaa agcggctcga tgacgctgtt gacatttaaa
    34741 agatttctcc cttccgtgcc cgtggccagg gttaatacgc cgggcgggaa gcttcgctcg
    34801 gacaacgttt tttccagagc gtgaacaaag tttccttcat ggggttcgac tttttgccag
    34861 acaatccgat catcctcaag caagatgagc ttgatactgg acgatcctat gttaattccc
    34921 aaagtctgca tgagctctca tcaatcattt ttcaggtgtg tttagtttat ttcttttaaa
    34981 tattacgcca caatcaaatc gtcaagtttt agatgaaaaa aagagtctaa aaaaaacaat
    35041 cttttttaat atcaacaata tgggattttc cgatcccgga cgtgaaagcg tgtagaaaaa
    35101 gacggcggga aatcattatt taaaatgatt tcccgccacc ggtgagcaat gatttctaat
    35161 ttttttaatg gccttcttcc atagccccgg caatatacat catggccaga agcatgaaaa
    35221 cgagcgtctg gacaacggat gtaaaaacca ttaacacaaa cacgggaaga ggaacgaacc
    35281 agggaaccag aaagagaacg accagaagga cgatgtcttc acccttgata tttccgaaaa
    35341 gacggaaagt aagggaaagc ggtcgcgaaa tgtggctgat gatttcgatc ggcagcatta
    35401 agggggtcag ccaccatacc ggccccatga attgtttgaa atattttaag ccgtgttcct
    35461 tgatgcccac aacatgggtg gaaagaaaga caatcaaagc catcgccgca ttggtgttga
    35521 tattggatgt cggagattca aatccgggaa cgattccgat gaggttcgac acaagaataa
    35581 agagccccag agtggagatc aaagcaaaaa actttcttcc attgtgtccc atgatttcat
    35641 tcagcagacc atcgaatccg ccgataatga cttccatgac gttttgagtt tttccgggat
    35701 aaacttctgt acgtctggtg gccaaaaagg aaaaaaaagc taaaagagcc attacaaaca
    35761 atgtgttaaa gacaatcgtc atgtcaatac ccacatcttt cagccattgc gtgtgaaaaa
    35821 atattaaggg ttccatctat ttttatacaa cctcctcaat aaagtttttt tttgccaggg
    35881 cgataatcat cgttgccaga atattgatta tcacaacgga aagtccgata agcagtccaa
    35941 tgatgtcaac gacattattg gcgattaaaa gatacagcac aacggccatg atggccaggc
    36001 ggatgtaata ttttaccatc acagtgcctt tgggattccc ttgcaggttt tgaaaaatac
    36061 cgcgtaaccc ccgggccatc caatgaaaat tgacaatact gacaaaaccg ccgagcaata
    36121 tgccgagaga aaattttaac ggagctaaaa acagggatgg gatgaaaata acggccagaa
    36181 tgatccagtt ggtgatttca atcctttttt ggagcgggtc tttgacgatg aggttcactt
    36241 tttaaactct ttataatggt ttcttcatcc gtaaaattct tttgaactaa aacatataaa
    36301 ttacgaaatc cggccgctaa ccctatgaat aaaaaaacaa ccgtaaaaac atgaccggaa
    36361 tcgaattttt tgtccaaata cgaccccagc agaagacaac cgaaaactgc cagaatcatg
    36421 gcaatgccga tgctgctggc aaaagccatt tgcatcgcat actttcgcgt ttctttatcc
    36481 attcgctgat tggctcctta gcacctgcgg ccattaaagt caataaaaaa atgaccggcg
    36541 ttcatttttt taacagatga cgatttttct tataccaatc aatcgtcttg gtgagtcccg
    36601 tccgaatatc tgttttggca ataaatccga attctttcaa tgctttggat gtgtccagca
    36661 tacgcttagg ctgtccatcg ggcttggagg aatcccacac aatccggccg gaaaatccgg
    36721 tcaattcggc aatcagagtt gtcagatctt ttatggaaat ttcaaacccc gatccgagat
    36781 tgacagggtc gcttttgtcg tacagtaacg tcgccagata aatggcttct gcggcatcct
    36841 ccacatagaa gaattcccgg gtggccgagc ctgtgcccca cacctccagc tgatcggatt
    36901 gacgatcgat ggcttcaaag cattttttga ttaatgaggg aatgacatgc gacgaacggg
    36961 aatcaaaatt atctcccggc ccatacagat tgaccggaag caaaaatacc gagttaaacc
    37021 cgtattgttg ccggtaagcc tgcgattgca ccagcatcat ttttttggca agtccatagg
    37081 gggcgttggt ttcttccgga tagccgttcc agatgtcttc ctcgcggaaa ggcgtcgggg
    37141 taaacttggg gtaggcgcaa acggttccca gggcgacaaa tttttcaatt tttctaatgt
    37201 gtgcttcgtg gagcaactgc gctcccatga ttaaattatc ataaaaaagt tccgccggtt
    37261 tttcctgatt gaacccgatg cctcccactt tggcagcaag atgaatgacg acatccggct
    37321 tcgtttcctc atacattctc cggatcgcgt taatgtccgt caggttatat tccggaaggt
    37381 cggctatttc gatattccgg cagcctttgc ttttgaattt tttcagcaga tgcgttccca
    37441 gaaatcccct ggcgccggtg atggtaatcc gtttttgagg tagcattggc aaagccttaa
    37501 tggttgaaat tttgattatt ggaaacgaaa cccgcatcca ggagcgtttt ttctttttgg
    37561 gcaagctcca tgtctgatgc gatcatcata tcgacaaggt tctcgaagga cacccggggt
    37621 tgccagttca atattttttt tgcttttgtc gaatcgccca gcagagcgtc gacctctgta
    37681 ggacggaagt accgtttatc gatttccaca taacgtttgt aatccagatc aagtttttga
    37741 aaaaccattt ctgcgaattc ccggacggaa tgggtctctc cggtggcaat gacaaagtcg
    37801 tccggcgtgt tctgctgcag catcagccac attgcttcca cataatcccc ggcaaatccc
    37861 cagtcccgtt tggcttcgag atttcccaga tacaacttgt ccttgagacc gagtttgatc
    37921 tgggttgccg cacgggtgat ttttctggtc acgaatgttt cgccgcgccg gggagattca
    37981 tgattgaaaa gaattccgtt ggccgcaaaa atgccgtagg catcccgata attctgcgtg
    38041 atgtaatagg aataaacctt ggcggccgca tacggactgc ggggctggaa aacggttttt
    38101 tcatgctgcg gcggcggtgc ggccccgaac atttcgcttg atgacgcctg gtagaatttt
    38161 gtcgtaatgc ccgttttgcg gattgcttca agaattctca gagttcccag cgcggtgacg
    38221 tcgccggtgt actcgggcat gtcgaagctc acgcgcacat gactctgtgc gcccagattg
    38281 taaatttcat cgggtttgac ggaatggagc aaatccatca attgcgcgga gacgctcaaa
    38341 tccccgtagt gaagaaacaa tcgggcctcg ggatcgtgaa aatcatggta gaggtgatcg
    38401 atgcgttcgg tattgaatgt gctcgaccgt cggatcaatc cgtgcacttc gtatcccttg
    38461 tgcagtaaaa attccgctaa ataagaaccg tcttgtccgg taatgccggt caggaaagct
    38521 tttttcatct ggactcctga atctttgaaa ttattttatg tcgcttcata ccggattgag
    38581 gaatgcttgt caatgaaacg attaaagatt tctcaatgtt tttgcgcagg cctgaagggt
    38641 tttctgaaga tctttttcgg tgtgggcaaa agacagaaag cccgcctcgt attgagaagg
    38701 cgcgagccag atgccgtttt tcaacatgcc gcgaaaaaac cgggcaaaca tgtttgtgtc
    38761 ggatttcaat gccgtttgaa gatcaacgac agggccttcc tgaaaaaaaa cggtgaacat
    38821 cgaaaaaaca cggttgatgc agacaggaat tccttttttg aaaaacattt catgcatggc
    38881 ccggcaaagc tgatctgtct ttttatccag agaggcgaag gatttctttt ttgccttcaa
    38941 aagctgaagc gttgttaaac cggcgatcat cgccacaggg ttgccggaaa gcgttcccgc
    39001 ctgatagaca tctccggtcg gcgccagccg ctccatgatt tttttacgtc ctccgatcgc
    39061 tccgacgggc aatccgccgc cgatgatttt acccagacag gtcaaatccg gtgtgatgcc
    39121 gacgaggttc tgatacccgc caaacgtaaa acggaatccg gtaatcactt cgtcatagat
    39181 taaaacgatt ccgtttttcg ctgtcaggac tctgagttgt ttcagaaatt cttctcgcgg
    39241 cgcaacgacg cccatgttgc cggcgaccgg ctcaacaata acggcagcaa gttgcgatcc
    39301 ataccgcttt acggtttttg caagggcgat agtatcattg tagggcaggc tcagcgtgag
    39361 ccgggcgata tcatcaggaa ctcccgggga accgggaatg cccagtgtcg tcggtcccga
    39421 accggcttta atgagcacgg aatcgctgtg cccgtgataa caaccgtcaa attttactat
    39481 gagcttccgt ccggtatatc cccgggcaag ccttatggcc gtcatcgccg cttccgtgcc
    39541 ggaggatgtc atgcgtatcc gttcgatcga cggaagagct gaggaaatca tttcagcaat
    39601 ggcggtctca agcagtgtcg gggcgccgaa actggtccct tgtttcaagg ctgcccgaac
    39661 ggctgccgtt acgtccggat gggcgtggcc gagaatcatc ggtccccatg agagcacgta
    39721 atcgatatag gcttttccca aggtatcgta aatcctgctg ccttttgctt tcagcacaaa
    39781 gcggggatga ccgccaaccg atttccaggc gcgtaccgga gaattgaccc cgccggcaat
    39841 cacccggttc gccttggaaa attgaacgga tgttgtttcc ttcatcagaa atactccgga
    39901 gatgtttttt tgtattcttt gaggcttttg aggattaaat aatccccgat gcctgtagca
    39961 tttaccatgt ccttaatcaa tgatgaaatg ctccgcccgt ccgaatgcag cattgtaaag
    40021 agattgtatc tttccaaaaa cgcgggatgc ctttcataac agtgggagat gaaggagaaa
    40081 gatgcaaaaa gccttccggt tgtctcggtt tgggagggcg gcacagacca aagcaccaaa
    40141 gcgttctgtc cgtatccggc cttctgatgg cgcaggattg cgccgaattt ccggatgagc
    40201 ccttgttcac gaaactggtc aagagcggtc agaatttctt ttgccccggg cgttggagcg
    40261 ccggacaatt ttttaaacgg gaatttcgtt accggaatgt ctttctgaag caggcgtgcg
    40321 attttttttt gtttcgacgt taactttctc atgaatggat tctatactgt atcgcaaaaa
    40381 aaaatcaagt tgcccgatta atgaaaattt gtcttgacaa tttctttttg tgccgttaaa
    40441 agagaagcac tcaaagatcc caaaaaaaga tgaaacgcga aaggaggtgt gaaaaaacat
    40501 agcagggatt tttgcagtca gtattttaaa tttaaaagga gaaagttaaa tgaaaaagag
    40561 tttaatgtat gtcatgttgg caatggttat ggtaatcgtt agcgcacctt ttgtttttgc
    40621 ggctccggaa gcagcggctc aggctcccgc gggaacggtt gattatacga aagcaatcgt
    40681 ggccagcata tttgcattgg cagcaggtct tgccatcgga ctgggtgcaa tcggcgccgg
    40741 tttgggtctt ggtcaggcaa caagcggagc aagcaatgcc gtcggtagaa accccgaagc
    40801 tcagggcaaa gtcatgttga ccatgatggt cggtatggcc atgacggaat ccgtcgctat
    40861 ttatgctctg gttatttctc tggtaattct cttcgcgaat ccgctgttga agattatcgg
    40921 aatgtaattt aacaccgcta tatttaaaag gggaagaagc aaataacaaa ggacttcttc
    40981 cccttttttt atccaaaatt ctttaaacga aaatcagaaa tggacagcag ggtgatgacg
    41041 caaaacagca accggcggga ccgatcccgt cgtgtgtttt cccgcccgtt attcctcgac
    41101 agacactttt gcgcgtaaaa tctttttttc cgcctgttgg tgtttgtcgg tcagaatttt
    41161 ttctttggct cgtttgctgc gcttcctttt ctgccgtcgg attttttcga tcgtttgcct
    41221 ggcttcggcc atcaagcctt tctgctgtat ttctatttta tccaacagta accgtctggc
    41281 caaaaaacgg tttaatgatt gagaacgttc tttctggcac ttgacggaca gccctgtcgg
    41341 aaggtgacgc agataaacgc aggaggaact tttattgact ttttgtccgc cgggaccgga
    41401 agaacgaata aaggtttctt caagatccgt ttcggcgacc ccaagacttt tcatccggtc
    41461 ctggagcatt ttttcttttt ccgaagatac gggcatgacc aatgcaatga tacgtcatca
    41521 gggaatggaa taaatttctt catcaataag atgaatccag ttttgttcca aaatctctct
    41581 ggcttcgtca attttgttga cgccgagaat gacaatcgtc tcttcgccga aacgattgat
    41641 ggttgtgtag aggtagtgga tgttgatttc cgccttggcc agcggtttga gtatggcgtt
    41701 catgccgccg gcatgcaggg gaacctcgac cgccagaacc ggcgtcactt cgaaattcag
    41761 attatagctt ttcaggacgg cagccgcgcg atcgggatcg ttgactacga gacgaacctt
    41821 gctgtcatcg gcaatgctgg ccgcagagac ggctttggtg ctgatatctt ccagatcaag
    41881 aatatgcgtt aaggcggcaa aagagccggg tacattattt aaaataaccg aaatttgttt
    41941 aacaggcatc tatctgtcct caatcgttct tgacgtactc atatcctgca ttaaaggctt
    42001 tacggttcag ttcggcgagt gatttctttt tgctgcccat gatcgtttcg agacttttca
    42061 ggtagacatc aggaggaatc accgccgata ctttgataaa agcccccatc atgattgtat
    42121 tggatacccg gatattgccc aaatcctgtg ccatatcggc gcagggcata gagcacacct
    42181 tgatatcgct gcgatgcggt ttttctttga tgatggagga atttaaaaaa atggttccgc
    42241 cgctggaaat tttattttga aacgtaaaaa gcgacgggga attcatggcc accagaaagt
    42301 ccggctccga cgccacgggg gacgcaatgt cctcatcggc aatggccacg gtgcagttgg
    42361 cggttccgcc gcgcatttcg gctccatagg ccggaaggta ggtgacctga tagccctggc
    42421 tcattccgct gtgggcgaag ctcaccccca tcatcaaaac accctggccg ccgaatcctg
    42481 agaaaatcgt tttaatcagc atcagcgccc ctccgcagat ttttttgccg cggcaatatt
    42541 gtctttataa accccggggg gaaacgtctt gctcatcacc tcatcaatcc atttacagga
    42601 attgaaggct gtcatcttcc agttggtggg gcagggggaa agaatttcga tcagggaaaa
    42661 tccaaacccg tccagttgac attgaaacgc ttttttaatg gcttttttcg ttttgttgat
    42721 attgagaggc gaattcacca tgcatcgttc cagatacgta acgccttcca gttgcgaaaa
    42781 aatttcactg actttgacgg gatagccgtc cagcgttcta tgccggccgg caggggatgt
    42841 cgtcgttttt tgttccagca tcgtggtcgg agccatctgt ccgcccgtca ttccgtaaac
    42901 ggcattatta atgaatatga tggtgatatt ttcgccgcga ttggccgcgt gaaagctttc
    42961 cgcgatgccg atggctgcca gatcgccatc gccctgatag gaaaatacga cccggtcggg
    43021 cagagagcgt ttgatggctg aagccatggc cggaccccgt ccatgcagcg cttcgatcat
    43081 atcgacatcg aagtaattgt acgccagaac cgcgcaaccg gccggcggaa caccgatggt
    43141 gatgccccgg atatccagct catcaatgac ttcggcaata atcctgtgtg caatactgtg
    43201 accgcatccc ggacagtagt gaaaaacatt ttccttcaaa cttctcggtc gtttaaaaac
    43261 agttttaacc atatccgcca acctttgcat ccctagtcga ttttctcata gaatttggcg
    43321 atgacattgg ccagttccgc cggtgtcggc accgcaccgc ccggacggcc gtgaaattta
    43381 atatccgcgt agccctgcag ggacaggcgc acatcctcca gcatctgccc cgtgctcatt
    43441 tcaaaaacaa ggtaatgctt cacgatttcc gataaacttc tcagacgaag ttccggaaaa
    43501 ggccataagg tgaccggacg gaaaagtccg accttcaccc cctgggcgcg cagccgcttg
    43561 atggcgcctt ttgcgatgcg agctgcagta ccgtaagcca cgatcaccat ttttgcatct
    43621 tcgatcttat gttcttcaaa acgaggttct ttttgagtaa taacttcata tttgcgcgcg
    43681 agtttccagt tatgctcttc catgtcgatc ggatccagga tcaaaccgtg aatgaatttg
    43741 cttttgcctg tgccggcgcc ccgcagcatg aacttttcgc tgtacttgcg aggtttgggt
    43801 tttttgaaaa gcaccggctc catcatttgt cccatcatgc cgtcggccag aatcataacg
    43861 ggattccggt atttgtcggc aagatcgaaa ccctccatgg tcaaatcgga aagttcctgg
    43921 ccgcttgccg gagccaggac aatggttcgg taatcgccgt gtccgccgcc gcgcgtggcc
    43981 tggaaataat ctccctggga gggtctgatg ttgcccagcc ccggaccgcc ccgcatgata
    44041 ttgacgatca cacccggcaa ttcacaggcg gccatggacg aaatgccttc ctgtttcaat
    44101 gaaatcccgg gactcgagga ggaggtcatc actcttaacc cggccgcgct tgctccggca
    44161 atcatattga tcgcggccac ttcgctttca gactggataa aaattccgcc ttccgcatgg
    44221 cggatgtgct cggccatata ttcggtaagt tcgttttgcg gggtaatcgg gtacccggca
    44281 tagaaacggc atcccgcacg gattgcggct tcgccgatga cgtgatttcc ggacattaat
    44341 actttattca cgatatacct caattgcgac ttcaggacac atggtggcgc agattccaca
    44401 gccgttgcat tcctttgtct catcgatgcc gaccggatga tagctcaggg cattatattc
    44461 cgtggtggtc acgatatgac ccttgggaca aaaattgata cacaagaggc attccttgca
    44521 gatttccttg ttaataacga tatatccctt caaagtccgt tccctgaaaa attaaatttg
    44581 cgccagctat cttgcgtata atttcgagaa agtcaagttt tttaaagcca tctcgttcgt
    44641 aaattaaaaa aaagatgatc cgctacatca gacgaccgtc aacaatgatg tcatgtccgc
    44701 gcacggatat tttcttgacg gtgattttca ttgccaagtc cagatgccgt atattcaggt
    44761 cgccgaccgc ttcaatgccg cagccgatta ttttgggcgc aatgaccgtg accaggcgat
    44821 tggcgagatt tcgttttaag acggatgtga tgatctgggc gccgccttcg atcatcacgg
    44881 atgaaatacc ctttgcggcc agtcttttaa agagtttttt caaatcgaca cggccgtctc
    44941 tgtcggcgct gacggttaac attgtcacgc ccagggcggc cagttttttg tatcgggaat
    45001 cggacgctgt tttcagggtt gcgaccagcg ttggcgccgc agagagattc tgaaagattc
    45061 tggcatcggg agatactttt aatgccgaat caacaatcac ccgaagggga ttcctgccgc
    45121 ggacaagacg aactgtgagt tcgggattgt ccctttctac cgtttgtctg cccaccagaa
    45181 tggcgtcgtg ttcggctctg aggcggtgcg ataatctcag cgacgccggc gaactgatcc
    45241 actgagaatt tcccgttgag gttgcaatgc gtccatccag cgtttgagcg tatttaattg
    45301 taacaaaggg cagtcccgtt tccatgtaat gaaaaaaagt ttcattgagc agacggcatt
    45361 cttcttccag aatgcccgtc ttgacgtcga tgccgctgtg gataagacga ttcacgcctt
    45421 cgcggcacac caggggattg gaatcgattg ttccgataac aacacgggcg attttattat
    45481 gaatgattgc gtcaacgcag ggcggtgttt tcccttcatg acaacagggc tccagcgtca
    45541 cgtaaagggt cgaacctcca acgcggcctg cggcattttg aatcgcgttg atttcggcat
    45601 gatatccgcc aaagcactga tgatagcctt taccgatgat ccgtccgttt ttaacgagga
    45661 cagccccgac catgggattc gggctgacgc aaccccgcgc cttgcgggcg aggccgatgg
    45721 ctattttcat gtaatattca tcattcatgt gaggataatt accttattaa ttgaaaaatg
    45781 aaaagaaata tgttgtccat tgccgtaaaa ccatgttaag aaaacagcac atacacaatc
    45841 aggatttttt aaagaacgaa aacgactgtg acgtatcatt tcaaaacagg aaaaaagcag
    45901 ggaaaataag agcggcgttt tgaaaaatat tcaataattt aatgaattgt tgcagaaaat
    45961 tataccttga ctttgattat tttttgtctt aaaataatac aaagcttgaa aagggcaaac
    46021 cgtactgaaa agacgggacg caaagttact gacctaagtc ttcaataaga ggatagggtg
    46081 gcagaaccgc ctggcaactt cccgcaagtc cagccgtttc ataaaaacca ccctgagaat
    46141 gttaggacta aaaaacatgc atgaacagga agcatgatga gtttcgtaat gaacactctg
    46201 gcttttgttt tcggagctgc gatcggcagt tttctgaacg tctgtatctt caggctgccg
    46261 gctgaaaagt ccatcgtcgc acccgcctca agatgtccct attgtctgca cccgattcgt
    46321 ttctatgata atattcccat tatcagcttc atcttgctgg gcggcaaatg ccgtgactgc
    46381 gaaagcagaa ttgcctggcg ttatccgctg gtggagctca cgatggcact gctgtcggtt
    46441 gctttattta tgaaattcgg tttatcatta aatttctttg tctttttcat ttttacagcc
    46501 gttttgatgg ccattacctt catcgatctc gatcatcaga ttattccgga catactgact
    46561 ttgccgggaa ttccgctgtt ttttctggcg gccgtattta tcgtgagaat cccgtggctg
    46621 gaagcatttc tcggattttt aatcggcggc ggcgtgcttt ttgtcattgc gctgggttat
    46681 gaatttattg caaaacgcga aggcatgggc ggcggcgaca tcaagctatt ggccatgatc
    46741 ggagcatttc tgggctggaa atccctgata ttcattcttc ttttcagctc gctgacgggg
    46801 gccgtcatcg gtatcgcagc aatggtcatt cataaacagg atacaaaata cgcgatcccc
    46861 ttcggtccgt ttttatccgc cgccgccatt gcctatattt tcttcggcca atgttacatg
    46921 aatttatttt taatgggtta ttagttttta aaaaagcgaa aaaggcccaa agatggtgaa
    46981 aaagacaacg tattgatatt aagaattata tctggaaatt gcccgggaga tgttgtagta
    47041 attgataata catcaggaaa tacgaatcga gagggcggaa tattgaaatc ataaggaatt
    47101 tcaggaaaat ggcatatcaa ttgctgtaat tattttgaag atgcatttta tgatgaaaac
    47161 gttaaaaaac ccgatgaata gagggctaag cttggtagaa ttgctgatcg tgattgccat
    47221 tctcggtatt gtgggaacga ttgcctcttt cggctggagc cgttatgtca acaatgctga
    47281 tttgcgcacg ctgtccagag aaattacatc ggacattgcg aaaatgaagc aaagggccat
    47341 tgcggaaggg gtttgtttcc agatgacaat caatgtcgcc ggagcgggaa acagctatac
    47401 attggagcgg gcaaacaccg ccagttgcag tggcgcttct acagttttca cagtcctgca
    47461 aaccaagaaa cccgaggagc taggattgcg tgccggcatt aacataaaaa gcacaaccca
    47521 taatggaaat gatatagaat ttcagacaag gggcacaatt tccaatggac ggatcgagtt
    47581 ggaaaacagc agggggtcgc aagcaataat aacaagcaat atcacaggta aaactcatgt
    47641 tacatacaac atgcaataac aaaggcatgg gcatggtcga agtgctcatc gcgatcttcc
    47701 tgacctcggt ggctgtcctg tctattctgg cgcttcaacc atccggttgg aaagcaatgg
    47761 ccaaatccga ttttgccggg cgcgctgcgg aagttttata taatactctg gaagattatc
    47821 aggtgaggat ttcgaatccc tgcaatgccg tcaccaccgg caaccaggca cagcaaaccg
    47881 ttacaaccag cggtgagacc gatgccattc agggggatat tacttatacg gtcgatgtca
    47941 acatcgtaca gacgaatgtg gatccgcaga cgtttgttgt tactgtaacg gtaacgtggc
    48001 cgggcaacac aaggggaatt tcagaaagcc tgtccattac gagacaggaa atttacaaat
    48061 tcggatgtta gaagatgatg cggaataaac acggatttac attggtggaa ctgattgtcg
    48121 cattgtttgt cggcagcctg gtgctggtgg cgatctattc gaccgtgaac atcgcgcagc
    48181 ggtcatcttc cggtcttgaa cgaagagtcg ttgcacagca ggacgcgcga agcgctctgg
    48241 agttgatggc cgccgaaatc agaatggctt cattcaatcc gctgtcgagg gataacatct
    48301 ggatcgatgt gaataatttt gattcggatt gcggaggtgt ttcatttgta aatcagaact
    48361 atcggggcat cagacaggca gccgcaaacg ccatcgccat cgaaatggac atcaacggcg
    48421 acggcagcat cgatgatcct aatgaaatca tccggtaccg ttatgattca gcaaaccgtt
    48481 acattacccg gtcaaccaac tgcggagggg cccaggcgtt tctgggagat acaaacgcaa
    48541 atcaaaacat caagacggtt ctggtggaaa acaacaacgc gggcataccc gttttccggt
    48601 attttgacgg gtccggtacg gatatctcgg ccaatgtggt tgccaatccc gccgatccgc
    48661 tgatcggcat tcctggggtt cgccgcatag aaataacatt agtggccgac acctcgaatg
    48721 ccgatgtcgg atccaataac cgccgacgaa ttatttattc aaccagcgtt attccccgaa
    48781 accattttgc cctgatttat gagtaatgct atgtttgcaa aacgaaaaaa atatttaaaa
    48841 aatgaaaaag gatttgcgct ggtcgccgct ctgattgcct gtctgatcct gttggcgctc
    48901 ggcatgctgg tcatcaacat gtccaccggc gatcttttta ccacctccgc cgtcatcggg
    48961 aataaaaagt ctctggcggc ggcggaaagc ggcatttcca aaatcattgc cgatgtcgat
    49021 cccgataact ggacaacaac cgagcattac accgcggcca ccgacggttc gggcaacctg
    49081 tgcggaacgg atgcgtttaa tcatggctta tacgatttta tagatattac aggcggcaca
    49141 gatcccaata cggaatttgc ggtttgtatc ccgcggccca gcgcccagcc gccgcttccg
    49201 gcatcgggtt acggcctgag cggcggggga tcaagcgcgg tgggatatgc atatatgcgt
    49261 tacgattcaa cagttgtggg aagaaacacc tcccatgacg ccgaagcaaa agtcgatgtc
    49321 ggtgtgggat ttggtccggt accgctggga ggaaattaaa ggaggtaaga ttatgcgagg
    49381 aaaaaagttt ttgattatga tcgtcgcttt tataattatt ttatccgccg gcatttcaca
    49441 ggcagccgat gaagatctgt ggacggcggt aacagagccg gatgc
//
LOCUS       DAOSME010000011        47838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_968_length_47838_cov_20.945252, whole genome shotgun sequence.
ACCESSION   DAOSME010000011 DAOSME010000000
VERSION     DAOSME010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 47838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47838
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_968_length_47838_cov_20.945252"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLG98_02865"
     CDS             <1..687
                     /locus_tag="PLG98_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32222.1"
                     /translation="YGSISIDQRKGNFSDGEICYTYRPAATKIANYLFSLADVLELNS
                     SEKINPWSTIVLRLEEKSAATGPFSGQYVNDIKIYLGDTQGRATPTGNPLDMPRHGGK
                     RWSNPPVAGDVQWPAEAGWSTSSAASACCSDENINKDYFTLVRGWVFNTAASDVNANY
                     ALTGTAEESNSTIRTIEFTTHSMGTFNQNELGLWTSGNSSANNVYFDDFAVRLEEARS
                     VVGFTTPLME"
     gene            965..1195
                     /locus_tag="PLG98_02870"
     CDS             965..1195
                     /locus_tag="PLG98_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOE32223.1"
                     /translation="MEKIIKIHVKKLPEGVYLATSKDVPGLVAQGRTATETLEIARDV
                     AKKLLEARKERRGKIKISEIKDSFDYPMIVSA"
     gene            1792..3285
                     /locus_tag="PLG98_02875"
     CDS             1792..3285
                     /locus_tag="PLG98_02875"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOE32224.1"
                     /translation="MNVFVKKGMLAETKSQAVILTLFEDGKKLSGIALEIDKKSGGLI
                     SKLMADGDFEGKASQVAVAYPCGNLPSKRIALVGLGKQKEFNLEKLRAAFAKVMRHLR
                     GMNVKEASTSVNLNLIPGKKDQIVQAAVEGALLGLYQYTPYKTVGREEFKEMDEFNII
                     ADAKEYAVIEPSVKKAKIIADAVCFVRDMISAPSNEMTPSIMAQKAQEVAKRKNVTCT
                     VLDRKKMQELGMNALLAVASGSQEEPKFIILEYSGGRKKDAPVVLVGKGLTFDSGGIS
                     LKPSDKMEEMKTDMSGGAAVMGAIMAAADMQIPLNVVALIPASDNMPSGSAFKPGDIL
                     KSYSGKTIEILSTDAEGRLLLADALAYASKFKPEAVIDLATLTGACVIALGDDVIGML
                     GTGEKLKKEINRAAQDTGELVWELPLWEQYHELIKSDIADYKNAGNRAAGTITAAAFL
                     SKFVGDYPWAHLDIAGPAWADKDKPYIPKGASGIGVRLLVEFLRNRS"
     gene            3295..4284
                     /locus_tag="PLG98_02880"
     CDS             3295..4284
                     /locus_tag="PLG98_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32225.1"
                     /translation="MPPLLQIKDLRTVFDTVHGRIKAVDGVSLDIASGETLGVVGESG
                     CGKTMLSLSIMRLIPPNGKIINGEILFSGVDLLKLSEEDMRARRGKDIAMIFQEPMTS
                     LNPVFRVGDQIAEAIRLHQQLPAKQAMALSVELLREVGIAEPQKRARDYPHNLSGGMR
                     QRVMIAIAMSCHPQLLLADEPTTALDVTIQAQILDLISNLKQKNNMAVLFITHDLGVV
                     AQAAEKVAVMYAGKIVEYSTVENLFAKPLHPYTQGLLESIPARCSDAKEKQAYLKTIP
                     GSVPDLYRMARSCRFHDRCPFAFAECELQDPPLLAVEDGHLVSCWKYGSEKNN"
     gene            4300..5268
                     /locus_tag="PLG98_02885"
     CDS             4300..5268
                     /locus_tag="PLG98_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE32226.1"
                     /translation="MSSVLVDIQKVDKKYTLGGNVFGAQKRIIHAVKNVDLQIIRGET
                     LGLVGESGCGKSTLARLITRLENPTSGVIAYKGENIFTFTGESLKTYRRSVQIIFQDT
                     YSSLNPRKSALSMIREPLTIHRLGSKKEREETALSIMAKVGLKKEQAHRYPHEFSGGQ
                     RQRIGIARALVLNPELIIADEPVSSLDVSIQAQILNLLRDLKNDFHLTYLFVSHDLNV
                     IRYISDRVAVMYLGKIVELAGGEDIYNRPLHPYTRMLLSAVPSCDPGKRKTSIAIKGE
                     ARETDNEGCAFRNRCPYRIDVCDKTDPALDAINGQSFCACHRAGAI"
     gene            5344..5958
                     /locus_tag="PLG98_02890"
     CDS             5344..5958
                     /locus_tag="PLG98_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32227.1"
                     /translation="MREEKILIKNNALVIEALLHRASGERGAVICHPHSLMGGSMYNN
                     VVEALQEAFASEGISTLRFNFRGVGSSTGSYSEGVGEKEDIWAAGNYLKTSGLREIFF
                     AGYSFGAWVGAGVIAQPDHPFCSSVFVAPPIDYFEFDWPPLKNKIDLMICGDADSFCP
                     WEPLKDASKKINALLEVIGGADHFFMGKEKKLVILLRDYLAKKT"
     gene            6210..6410
                     /locus_tag="PLG98_02895"
     CDS             6210..6410
                     /locus_tag="PLG98_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOE32228.1"
                     /translation="MAEGKVKWFNEKKGFGFIENDEGGDVFVHYSAITGTGFKTLYEG
                     QKIKFDIEQGNKGPSATNVTPL"
     gene            complement(6552..7895)
                     /locus_tag="PLG98_02900"
     CDS             complement(6552..7895)
                     /locus_tag="PLG98_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE32229.1"
                     /translation="MNKRLDLGKEAILPLILKMSWPSIIAMTAMSLYNFIDTFWLARF
                     SSQALAALTVCFPIQLLAAAIGVGTGIGAGSFSARMFGAEKNLEAQKTAGQTFFLSML
                     FGFGSILFVLLFGDSMLVFFGASDEILPLSHDYLFLAVYSSPFLFFSMMTAIMLRVEG
                     RPLLSMNVVLIALISSAILDPFLIFGIGPFPRMGIRGAALAGIIAHLIMFILSLYFLQ
                     LKSSKYDFRLKYTRPDLQIIKSIYSTGFPSMVTNFVISIVLVIYNHVLGNFGFQAIAA
                     FGICFRVTGLATMVLFGVGAGLMPIVGYNLGARLFQRLRESVSVALKVSVVFSAVSVI
                     ILELFSSQIITFFSSDPHLTAIAIPALRIHVAALIFSAPIIIYVNMFIGLGKGTLAMI
                     ILFIRDILVFVPLLIILPLFWGITGAWMALPLAGFIELFLVIYWGEKELRKLETR"
     gene            7963..8874
                     /gene="miaA"
                     /locus_tag="PLG98_02905"
     CDS             7963..8874
                     /gene="miaA"
                     /locus_tag="PLG98_02905"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOE32230.1"
                     /translation="MAKNLIVILGPTASGKTRLAAQLACDFQGEVISADSRQVYRTMN
                     IGTGKDLKEYVIDGRQIPYHLIDIEDPENEFNVFEFQKRFYAVFSAIRQRKKLPVLTG
                     GTGLYLEAVLCAYDMPEAPINEEIRRELSEKSIEELQDLLCRLKPHLHNRTDLDDKKR
                     LLRAVEIEKARIRNNQDLRDRPEITAAVFGVRWERSVLRNRITKRLEERLENGMIEEV
                     AGLHAAGLSWLRLESFGLEYRFISEYLQQRLTFDEMKNRLNIAIHQFAKRQETWFRRM
                     ERKGVQINWIDNGDYLLLAEKVAKHLK"
     gene            8871..10226
                     /locus_tag="PLG98_02910"
     CDS             8871..10226
                     /locus_tag="PLG98_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32231.1"
                     /translation="MKKRSAFDDYLHTYSVGEKWKLCAVPDQNFSQVVVIPAYAEKDL
                     LFRTLASLAVNVPDALENSFILCVVNHKEDSPASVMENNKITMAYLNALIRRKSLKTF
                     QLTKDVTALLQCISDSKMKLGYIDAASRGYEIPRNTGGVGMARKIGMDMALRLLEKNA
                     PQNGAIVSLDADTLVEPNYLSVVRDYFRKNVKTAIMAYAHQMPENPAEQAAICCYEIF
                     LRYWVLGLRYAKSPWAFHSIGSTMAVAPQSYLAVRGMNKRAAGEDFYFLNKLAKVGRI
                     DYIRETCVHPSARVSTRVPFGTGKRIERFLAGDAAKEYYLYDPQIFSILSGWLSLVHR
                     MIDRDVDDILMEAKRIHPALKAFLEEARFPLRWSAIRRNTKDQETLLNHFHVWFDGFR
                     TLKMINYFSRVAYPPVDMFSALKRIFNLLDLPALSPEVAEDIPSLGEQMEILRFLRTI
                     T"
     gene            10383..10805
                     /locus_tag="PLG98_02915"
                     /pseudo
     CDS             10383..10805
                     /locus_tag="PLG98_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
     gene            10875..11384
                     /locus_tag="PLG98_02920"
     CDS             10875..11384
                     /locus_tag="PLG98_02920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE32232.1"
                     /translation="MKPTEKDINKFYNDNREKLFVEPESVHVRHILVSVGKDDSEKVK
                     AEKKAKIDDLRKQLVGGANFAEIAGQHSDCPSKQAGGDLNFIRRGQMVKAFEDAAFAQ
                     EKNAIGPVIKTEYGYHIVQVLDRRPAKKIALSEVKSKIADYLERQKKMEAFNNLMAKL
                     KAQAKITVY"
     gene            complement(11409..12227)
                     /gene="panB"
                     /locus_tag="PLG98_02925"
     CDS             complement(11409..12227)
                     /gene="panB"
                     /locus_tag="PLG98_02925"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOE32233.1"
                     /translation="MSTHSKITRKTTADIRKMKTTGEKIAMLTAYDYAMASILDESDI
                     DMLLVGDSLGNVMLGYDSTLPVTMDDMLHHTKAVARAVKKAMVVADMPFLSYQVSPEA
                     ALANAGRFLKEADAQAVKLEGGREFADIVHKMTYAGIPVMAHLGLTPQSVHQLGGYKV
                     QGKKDDAAETIMQDARILEESGAFAVVLECVPEKLAAEITSALSIPTIGIGAGVYCDG
                     QVLVVHDMLGMQEKFTPKFVKKYANLNLEIKKAVKTYIGEVKNSNFPDTEHSFK"
     gene            complement(12214..13110)
                     /locus_tag="PLG98_02930"
     CDS             complement(12214..13110)
                     /locus_tag="PLG98_02930"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOE32234.1"
                     /translation="MKKKELNNFAIIGTGMVGTAIGSLLKKSGYNVVSICDKSSTALK
                     RAVSYTGGKPCKTIPDAVGHADSILITTPDDHIASACREIASASSIAGKYVFHMSGAG
                     GLDLLESSKKAGAFVASIHPLQSFSSIDQAIKNIPGSYFGVTADRYSLIPAKHIIHKL
                     GGIPMVISNDQKPLYHAAACFASNYLVSLMNIVESIYRSIGIREKDAQRAYLPLVYGS
                     LKNIEKSGSISSLTGPIARGDYGTIRKHLRTIDKNLPRYSSLYASLGLITVKIAQQKG
                     TLNAGQARKINALLKGGKHEHS"
     assembly_gap    13233..13332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13706..14353
                     /locus_tag="PLG98_02935"
     CDS             13706..14353
                     /locus_tag="PLG98_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOE32235.1"
                     /translation="MMKKIDLMIFDLDGTIASTGDDLVLCINHTLKTLNLRERTKKDI
                     IGFVGDGISKLMERALGSRYREYQEEAMRIFSAYYAEHLLDNTKLYPHVEEVLRNFEN
                     KPKVVLTNKRYPYALMIVRGLKIEKYFTEVIGADSTPFLKPDRRIIDSVLEKHPAERH
                     RALIVGDGVNDIAVARNSGIMSCAFLNGLGRRDDLLKAKADYYCEDIREVNRLFR"
     gene            14356..15771
                     /gene="tilS"
                     /locus_tag="PLG98_02940"
     CDS             14356..15771
                     /gene="tilS"
                     /locus_tag="PLG98_02940"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOE32236.1"
                     /translation="MIKKVKQTIEKFDLLKKGERVVVAVSGGPDSTACLTVLASIAGD
                     MDLELIVAHFNHGLRGKESDRDEQFARRLAKKLGLVFYAGKLNKKIRVKGISPEDFYR
                     RQRYAFLEKTAAKHRAQKIALGQHLNDQAETVLLNLLRGSGLEGLKGFLPIREGRIIR
                     PLIEVSAQEIISFLEQSGMAYCRDSSNESSQYLRNQIRAELIPFLKGKYNPRIEENLA
                     QMAEILRREDDFIRQHAAAAAKKTMQQKKAGSVALNIHAVKKIPEAIRWRLLKMLLED
                     LTPSGDGISFLHVNAVNDLTQKNASGKKITLPAGIEARREYDQLILEKKRTRPKKNQY
                     AYVMSVPGTVHVKERGLTIKATLVKKIKTDLSRMKNTACLDWSKIAPPLVVRNRRDGD
                     WIQPLGMRGRQKIKKYFIDHKIPVAQRDDLLLLVDRLSVVYIEHRHISDRVKISPATK
                     SVLKLAITPDRPVGKGSKPPG"
     gene            15849..17672
                     /gene="ftsH"
                     /locus_tag="PLG98_02945"
     CDS             15849..17672
                     /gene="ftsH"
                     /locus_tag="PLG98_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOE32237.1"
                     /translation="MNQFQKNIALVLTVLLVFLLVWQLYNQPKTGTKEIIYSEMLTLL
                     DKGEISEVTIQGDHINGKLKNNSLFKSFAPKDDQLVEKFSGKGVKITAKPVEESAWLT
                     ILISWAPMILLIGIWIFFMRQMQAGGGKAMAFGKSKARLITDKSKRVTFADVAGIDEA
                     KSELEEVIDFLKDPKKYTKLGGRIPKGLLLVGPPGTGKTLLARAIAGEAEVPFLTISG
                     SDFVEMFVGVGASRVRDLFSQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLN
                     QLLVEMDGFESNEGVILISATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREKIFEVH
                     ARKTPIAGDVDFAVLARGTPGTSGADIENLINEAVLNAARANKEKVNMSDFEFAKDKI
                     LMGSERKTMVISDEEKRNTSYHEIGHTLVARLIPGTDPIHKVTIIPRGRALGLTQQLP
                     VDEKHTYPRRYLENSISILLGGRAAEELVLKDFTTGAGNDIERATNLARKMVCEWGMS
                     DKMGPLSYGKKEEQIFLGREFATHKDYSEETAKNIDAEVMAIVARNYERSKQLLTDHI
                     DLLHKISGELLEKEVLSGAEIDTLIGDALPKAHRSEPQTSV"
     gene            17735..18940
                     /gene="folP"
                     /locus_tag="PLG98_02950"
     CDS             17735..18940
                     /gene="folP"
                     /locus_tag="PLG98_02950"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOE32238.1"
                     /translation="MKIIDIQSTQEAAALFRRIGVDAYGIGAMAPKAVNLNILLEKQP
                     CKIANILKQEMLALGGDAAVARGSVACSVETTDVLMMGTLKQLSRLAAKIARQPFGLD
                     SIARDILRILKNAGQKRFILKTSRRKITLGDRTRIMGILNVTPDSFSDGGTFLSAERA
                     VEYGLQMEAEGADILDVGGESTRPGSAAVPVHVELKRVLPVIKALSRKIKIPLSIDTK
                     KARVAREAVEAGAEIINDISALNGDRKMAGTAAETGAALVLMHMRGNPENMQKGNLAY
                     DDLLGDITDYLSASIGKAIRAGVSKDAMVIDPGIGFGKTVADNYRIIRRLADLRTLGM
                     PILVGPSRKSFIGNVTGDEPRQRLEGTAATVSAAIMNGCHIVRVHDVAAMKKVAAVTD
                     AMTHPRKVI"
     gene            18940..19323
                     /gene="acpS"
                     /locus_tag="PLG98_02955"
     CDS             18940..19323
                     /gene="acpS"
                     /locus_tag="PLG98_02955"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOE32239.1"
                     /translation="MVYGIGVDLVENRRMERIIDRWGVKFLNRIFSTEEMAYCGKHAY
                     PVTHYSARFAAKESFLKALGIGLGMGIKLSDISVVNHEGGQPFLNLSGDAQSYIQKRR
                     IHKIHLSLTHTKKYSAAMVLLETID"
     gene            19343..19831
                     /gene="tsaE"
                     /locus_tag="PLG98_02960"
     CDS             19343..19831
                     /gene="tsaE"
                     /locus_tag="PLG98_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOE32240.1"
                     /translation="MSQDDKTVEIISVSTENTMEIGRSIGEGLQDGDVVALSGELGSG
                     KTCFTGGLARGLGVDEKYRITSPTFTLINEYPARHRLYHFDVYRISSYSELDDLGCEE
                     YLGTEGVVVIEWAEKIKKILPEKTVYIDFEYVDENRRKMIIRASADRLNELIKDIKTE
                     GV"
     gene            19834..21054
                     /locus_tag="PLG98_02965"
     CDS             19834..21054
                     /locus_tag="PLG98_02965"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOE32241.1"
                     /translation="MALIVQKFGGTSVANIEKIKNVAEKVIKVKQAGHQVVVVLSAMA
                     GETDRLINLAHSATDLPDEREYDSLISTGEQVTVTLLAIVLNAMGCKARSFLGFQVKI
                     LTDQAYKKARISLIDTENIKKELKKGTAIVIAGFQGVDKDNNITTLGRGGSDTSAVAL
                     AAALRADQCDIYTDVDGVYTTDPNVCNKARRLEKISYDEMLEMAMMGAKVLQPRSVEM
                     AKKYNVPVYVKSTFSDKGGTLVTREDKDMEREIISGVTYDRDQVKITVVHVPDQPGVA
                     ASLFSPLSDKNIFVDMIIQNASSEGYTDLTFTVSKKDLKEAQKIVESTAKKVGAKKIE
                     VDDQVAKISIIGVGMASHSGVAAKMFKTLAAEGVNIMMISTSEIKISCIIQRKYMELA
                     VSVLHDAFGLDKKK"
     gene            21026..21622
                     /locus_tag="PLG98_02970"
     CDS             21026..21622
                     /locus_tag="PLG98_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOE32242.1"
                     /translation="MLSDWIRKNNIVFISESRLKGLIVLSLMVAVIPFVSLMITSWTP
                     YRIPVYADTCDHCCVIEIVGDDQRAGIYHVPSGMSVNRLLKTAGVEKQFEKDFTVKTG
                     MKLVIRSASDRPGITVAEIQNTAKISVGLPIDVNKAGEEDLILIKGIGPVTAKRILEL
                     RTRLNGISDIRQLMEIKGIKEKRMKHIEKYLYVEKKNV"
     gene            complement(21624..22253)
                     /locus_tag="PLG98_02975"
     CDS             complement(21624..22253)
                     /locus_tag="PLG98_02975"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOE32243.1"
                     /translation="MQNNIYRLFKLHITLFSKIVIAALLFFFAAAFTDIARADIYKYE
                     DNEGVLHLTNVPSDTGAKYVMVMREKRILFQPQLDVNKYDHIINKTADKFKLDSALIK
                     AIIKAESNFNHRAVSPVGAQGLMQLMPKTASQLNVDDSFHPEKNIEGGARYLRYLLNL
                     YKGNLSLALAAYNAGEGAVAKYNYNIPPYRETQNYVRRVLSFYRSFSGN"
     gene            complement(22379..23131)
                     /locus_tag="PLG98_02980"
     CDS             complement(22379..23131)
                     /locus_tag="PLG98_02980"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOE32244.1"
                     /translation="MQLSLENKVALVTGGSRGIGRAIALGLAKSGADVVIASRKIGDL
                     ENVAGEIKSLGRKCLPVATHIGRLEEIKNLVDKAAAEFGRIDILVNNAATNPTMVPAM
                     DIDDRAWDSIMNLNLKGLFFLSQAVARIMKEKGGGRIINVASVAGLSPDILPVYSISK
                     AGVIMATKVMAQQWAIYNIRANAIAPSLTKTKFSEPLWTNQDILNIAISRTPLGRPAE
                     PDDMVGAVIFLASDASDYITGQVLAIDGGMTI"
     gene            complement(23158..23619)
                     /gene="ybeY"
                     /locus_tag="PLG98_02985"
     CDS             complement(23158..23619)
                     /gene="ybeY"
                     /locus_tag="PLG98_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOE32245.1"
                     /translation="MKIQIENRQTLHKIERRKIRSAVSKIFKILECTDRHISILFTDD
                     EQIREINRCYLGRDKATNVISFSMREGEYGHINPQILGDVVISVETARRDAIRAKLML
                     EEEIDFLLIHGILHLLGYNHENTSRKEAKIMYDKERDVFRQMNSRKNMPAQ"
     gene            complement(23576..25936)
                     /locus_tag="PLG98_02990"
     CDS             complement(23576..25936)
                     /locus_tag="PLG98_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOE32246.1"
                     /translation="MKYFESLRIKMTEFFRNYLEKAAPYFGFVRNSLFQRWMIAVVLC
                     LILALIMAPELNFSKPQFRIGMIAPKNIKADFAFLVEDPQAAEEKKIQDAENINPIYN
                     YNSQIADNIQKKIVKAFSLAAERYNNVPEKQQQPDKIMDLQKEKKRLENNLGAYLTPE
                     EFYILNEYRFSEELQNKFSQLIISFYDNRFIASDAFGKSEKQKGVVIRNLRTQISEDI
                     RDTSALLNMNEIDDPLLKTINMIFRNDEKPLKDAAFSITKKIIEPNLAFNKGATEKKK
                     MAIIGDAAATFFQVQKNEMIVREGEKIGYLELAKLNAYFKDDQDNRFTRVAIVLGFFF
                     TAMFLSIVLYLWRTRNWVKTSDRSNLDLLVFGIIALLQILFIKIGIFMSIAINRSFSF
                     IPVDACYYAIPFAVGAMTIAILINRNVALIISALTSFLIGFLFDEKIVFPLFSFLGSI
                     AASYQIVRIRQRSVFLKIGLFLGIINMAAIVCLNLITGNFFNDLPIRLIMGFIGGIVT
                     GILVAGITPLFESLFGFITDIKLLELANLNQPLFQQMIIEAPGTYHHSIIVSSLAESA
                     AEAISANALLTKVSAYYHDIGKLKKPQYFIENQPNSDNRHDKLSPKMSSLIIMSHVKD
                     GCELAAKAKLGRQIINIIRQHHGTSIISFFYDKAKKDKNESLRSLSENDFRYPGPKPQ
                     TKEAGLIMLADVIEASSRTLTNPTPARIKSLVRERIEGIYMDGQLDECELTLSNLNTI
                     AETFTKILTGIFHHRIDYPEAPQKEGNGKKESNENTNRKQANPAQD"
     gene            complement(25939..26937)
                     /locus_tag="PLG98_02995"
     CDS             complement(25939..26937)
                     /locus_tag="PLG98_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOE32247.1"
                     /translation="MIKALKNEDAEKIVFADQHLLKNLCGEHDKNLKFIEKLEPVKVK
                     AWGNSVTISGRNGNVHKIKTMLQQMYALMEKGWHIQSADIDYAHRILTEDVTFELASI
                     FLNTVFISSQKRVITPKSIAQRLYIEYMRNADMVFSIGPAGTGKTYLAMAMAVSCLME
                     RKVQRIILARPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDMLDMERATALIHKGL
                     IEVAPLAFMRGRTLNDSFIILDEAQNTTSEQMKMFLTRLGFGSKAVITGDITQVDLPT
                     EKVSGLIEAENILKKSAGIRFVHFSEIDVIRHPLVQDIIKAYAHLTKSKNTNHHNV"
     gene            complement(27105..28349)
                     /locus_tag="PLG98_03000"
     CDS             complement(27105..28349)
                     /locus_tag="PLG98_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOE32248.1"
                     /translation="MIDEKLFQKISQRIDAYRDGMIELQTALCAHPAVGPENGGDGEL
                     VKANFLKDRLLQMGFKNFQHYDAPDNRVSSGIRPNFVTTIEGKNKNRFVWIITHMDIV
                     PPGELRLWSSDPYKAYVKDGRIYGRGVEDNQQDLVASIFAAKALMDEGVVPENSIKLA
                     FVSDEETSSSKGLFYMIDSTDRLFQDNDWIVVPDSGNAEGSLIEVAEKSMLWICFKTK
                     GRQCHGSTPHLGNNAFVAGSHLVTSLIKLRKMFPDTDPLFDPPQSTFEPTKKEANVGN
                     INTIPGEDVFCMDCRVLPEYDLKDVMNAIQKICRKIEKKFNVRIETSMVQYVQSAKPT
                     PSDAPVVLALQDAIDAVYHVKAFTGGVGAGTVASYVRKKNYPVAVWSKTNQFAHQPDE
                     NCPVENMIGNARVFAYLFLRKS"
     gene            complement(28371..29813)
                     /locus_tag="PLG98_03005"
     CDS             complement(28371..29813)
                     /locus_tag="PLG98_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOE32249.1"
                     /translation="MDKMISKIYPVFLVGVFLFLMMATVRPSSANQADAARPSPDQLQ
                     YEPLFFELPKAHRVVLENGIILYILEDHDLPLVTINAFVKTGSMHDPAGKEGTAELTA
                     QVMRTGGTTRLSSREIDRQFDLIAASASIAMAMESAHVGFSVLTTHLDKGLDLLSQIL
                     IHPVFEQGKLELARQLQLEEIRRIKDDPQKLAMREFSRLIYHQDPRGRFPSSASLTEI
                     KRDDLTAFHSRFFLPNNIMFAVSGDITKEKAVEKIKQYFGGWNPKNSATDFPAPVQQP
                     QTGMYFIEKDIPQSTIISGQFATGKNHPDFHAFTVLDFIVGSGGFPSRIMSTVRNNEG
                     LAYSAGSFYRARPDYGIFGSYAFTKTSSTMKALSLINTVLENIKAVPVAGQELTWAQK
                     SINDGFLFSFATPEQIVRQQMTIEYEKLPPDFLTSYREKINRVTVQDLHHAAVKYLDQ
                     TKNTVLILGDGKNPDHPLPPGGYTVIAPID"
     gene            complement(29820..31325)
                     /locus_tag="PLG98_03010"
     CDS             complement(29820..31325)
                     /locus_tag="PLG98_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOE32250.1"
                     /translation="MKACKILKAALLPAFIFIFIAAGSGHASDLKEKVLKQKLPNGIT
                     VLMLKRHLSPTVSLYIRYRVGAVDETKGESGAAHFLEHIMFKGTTSIGTNDYQAEKAL
                     MEEIKNAGRRLDAEKRKGQTADPETLANLSAVLKKLQDEHKKYFLPNEIDRLYTENGG
                     LDMNASTGQDVTSYHVSLPANKIELWARIESDRLLHSVFREFYTERDVVMEERRQRVE
                     SDPEGKLYEKFMSAAYTLHPYGRPVLGWTEDLMNLSPDELEKILEKYKDPRNIVIAVV
                     GDIKPEPTLKLIEKYFGVIPSAAEKTESYIPAEPPQTEERRVNVAFDANPSLIIGFRK
                     PNAPAREDYVFDVIETILTRGRTSRLYTTLVIDRQIAQSVSAVNGLPGSRFPNLFALF
                     AQPRHPHSNIELENDILKEIEKLKTQPVSDEELMKAKNHIKMSYLQSLDANADIASIL
                     SYYEVLLGDYRYFADYLDNIDKVTAADIRQIAARYLNSNNRTTAFLNKEGG"
     gene            complement(31312..31548)
                     /locus_tag="PLG98_03015"
     CDS             complement(31312..31548)
                     /locus_tag="PLG98_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32251.1"
                     /translation="MKVLIWIKNVLILLVSLFFLLIGINTLMGSYRINNPMEFLMYFF
                     SASLLILVCIVGILYFIFRLFPQKQEGLNKDESV"
     gene            complement(31545..32912)
                     /locus_tag="PLG98_03020"
     CDS             complement(31545..32912)
                     /locus_tag="PLG98_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32252.1"
                     /translation="MSRKILLINPWIHDFAAYDFWIKPLGLLYLGSLLRKNNHRIDLI
                     DCLDPFHPQMAASGLKKPRRYASGDGKFYRQIIDKPAPLKWVDRSYCRYGILPDVFAA
                     ELKMHRDADMILMTSMMTYWYPGVFEAIRIIKDTLPQIPVVLGGKYATLCYDHASNFS
                     GADYVIAGAGEKQILDLLEKVFSEKTLFVPDENNLDSCPYPAFDLISKTGQLPVITSR
                     GCPYRCSYCSSHILTPGFRRRDPVKVADEIEYWHRKTGVVNFSFYDDALLVNPEAMIV
                     PLLDELQRRNLSCRFHCPNGLHLREITKELAERLYHAGFKTIRFGFETSDLDRQRQTG
                     GKVKNEELRQAVKHLKNAGYKTEDLGIYLLCGTPGQTVREVINSIEFVQECGAKPLLA
                     EYSPIPGTAMWQEAVACSPFDILHEPLYHNNSLLPCGNDDFSFARYTELKKKLKNGSR
                     TGAPS"
     gene            33110..33865
                     /locus_tag="PLG98_03025"
     CDS             33110..33865
                     /locus_tag="PLG98_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOE32253.1"
                     /translation="MKKTTLTSIFFRSFFIHATLNFRRMQNMGFGMALIPMIRELRLQ
                     KKDAVKILTAHLQMFNTHPYLSAPIIGSIVRLEEDHVAKGENSDTASIRQSLMASYAA
                     IGDIFFWGAFRPFAAIVAVLLVHIGLAMAPVIFLLIYTPAHLWVRLKGFIEGYRKGKT
                     GFEFIRALNLPAAAVRIRWITLMVLTGLMIWLARDGGYWPFGETYGVVAKLIALATVM
                     LCYILIKKGISQVYIVYGAVLAFVIISWTGWIS"
     gene            33862..34131
                     /locus_tag="PLG98_03030"
     CDS             33862..34131
                     /locus_tag="PLG98_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE32254.1"
                     /translation="MTETKTLRLKNKLGMHARAAASFVKVAQQFQSEIYIEHNGQTVD
                     GTSILDILTVACPYGGELTIKADGADAAAAIEELVKLIENKFGEE"
     gene            34139..35920
                     /gene="ptsP"
                     /locus_tag="PLG98_03035"
     CDS             34139..35920
                     /gene="ptsP"
                     /locus_tag="PLG98_03035"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOE32255.1"
                     /translation="MSPDQTTKTFVLKGVGVSPGIAIGKCYRYDPLDSQISFYKLKDE
                     SLIPEEISRFKNALKESEQQLLKIQKNLKKANVAEPVYVIDVHILILKDKIFINRTIK
                     YIREMSVNAEWALRMTLDHYKEVFEGVEDDYISSRVSDVQYVVQRILRNLSGGKSEIV
                     WETENRGVIIVSHDISPADTAQMKLDKILGFATDSGGRTSHTAIVARSLELPAVVGLD
                     DITRFVHEDDDLIVDGTAGMVVINPYPDMLKRYEEKKRHYDAANDEYLKYGRQPAVSL
                     DQHRIQIGCNIEFIEEIPSAVAHGAENIGLYRTEFLYIYRENLPTEEDHFTNYRQVVT
                     NKNLAWSTIRTFDLGGDKFPNYQKQSKEMNPQMGMRAIRFCLKEVDIFKIQLRAIWRA
                     SALGNIKILFPMISSVDEIREAKKILEEARRELIDEGMKIPEKIDIGAMIEVPSAVVI
                     ADKIAREVDFFSIGTNDLIQYSLAIDRANERLTYLYEPLHPAVLRQIKKVVDAAHKAK
                     IEVSMCGEMAGDPLCVLVLLGMELDELSMNHLAIPRIKKIIRESTLKESKSMLKRALS
                     FNTASEVRSYVQKYMARRFPEDFKTED"
     gene            35964..38585
                     /locus_tag="PLG98_03040"
     CDS             35964..38585
                     /locus_tag="PLG98_03040"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOE32256.1"
                     /translation="MEENNHPVKSNQGIQYYSGLLYDNQNFLQFRLAKTFLSRVSIAD
                     EYVENIKKLSGEGVVVYALNQRSELNSLILYELLQRNDMPVPVYCHGMNMSLAHPLPQ
                     MLKFFWSSLKRRMGRERKAWQGKLAFIEKTIMEKKSIVIHLGESEFIEDRSAESAMST
                     LLDIQKKAGFPVFVVPMLVSYGRRREKEDEGLINILFGQTEHTGALRRLITFIRYSRS
                     AFVIPSEPVNLSEYLKTTKKTATDAMVHELRGELIDRIDKEKTAIVGPALKSREELIG
                     MVLSDESLKAFIEKHAQEEKKDKARIKKDARRYLYEIAADYSETFIAIWKKVLTWLWN
                     DIYDGLSIDVEGMAKIRNISKKMPFVIVPCHRSHIDYLLIHYVFYINNIQLPFIAAGN
                     NLSFFPMGYIFRKSGAFFLRRSFRGNKVYSEAFTKYLAILLKEGLPLEFFIEGGRSRT
                     GKMVMPKYGLLSMVLQAYQEKYCENFAAIPVYIGYDRVIEEKSYLKELSGETKTPENT
                     AEIIKTSKILTKRFGRVYVNIGEPMIMKDYLAQQAKPLEDMSLEERQSLYRKIGYEIV
                     LEINNVSVVTPFALVAAVILSHDRRGISYNDLLYVLNEFYEYLTVKQVKFAETFAHRD
                     KAIIDALNMFVQAGFIEKIEAEKDEVEEMQEVVYSLKEERRLNLEYYKNNILHFFVPL
                     CFVATSIIKNNEDLISLNRIQGDYGFLKKLLWNEFIFDKHKDEAEEVNEVLSYLYDRK
                     IIVSVEQDGQTLIEIKGRGNKRLKPLADLIHNYLESFWIVMRSCLYLKKNPLPKKEWL
                     KKIMALGDRMYRKGEVLRKEALSQANYQNVIIYLEDADLIKSFRDEKIDKKDVLYTLA
                     ENRAEMEVLRRRLFRFL"
     gene            complement(38698..40011)
                     /gene="eno"
                     /locus_tag="PLG98_03045"
     CDS             complement(38698..40011)
                     /gene="eno"
                     /locus_tag="PLG98_03045"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOE32257.1"
                     /translation="MPEIIHIHAREILDSRGNPTVEAEVTTLSGITARAAVPSGASTG
                     EHEMLELRDGNKKRYNGKGVEKAVDNILHKIGPEIIGMDCRRQRDIDFTMIEMDGTAN
                     KGKLGANAILAVSMACAKAGAEASGLPLYRYLGGVNSHVLPVPQSNILNGGQHADNNV
                     DIQEFMILPVGAPNFKEGIRMNAEVFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEA
                     LACIMTAIDKANYKAGRDIMIALDCAASSFYEKGKYILAAEAKPRKSSEELVKFYAEL
                     VAKYPIISIEDGLAEDDWSGWKVMTEELGGTIQIVGDDLFVTNVKRLNKGIDLGIANS
                     ILIKLNQIGTLTETLETMSRAKECGYTCVVSHRSGETEDTFMADIAVATNCGQIKSGS
                     LSRTDRLAKYNQLIRIEEELGDAAVYRGKETFYNMKQPAKSGRKR"
     gene            40158..40916
                     /locus_tag="PLG98_03050"
     CDS             40158..40916
                     /locus_tag="PLG98_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32258.1"
                     /translation="MNQDTKDRNTKLFLHMKKWLEKAVLDYKMIQKGDRLLIGVSGGA
                     DSLALLDLLDSPMLFIPDFSFVAVNIDMGFDPAYKKYDRLEKYFQKNNYPYVMEKTDI
                     GTVAHSDVNKKNPCFLCSRLRRKRIFEIADAEGCNKIAFAHHRDDIIETLLINMFYGR
                     EISTMMPNQSIFGGKLHIIRPLVYLSEALVKKYSRERRFPVLKNDCPTSKTSKRVYIK
                     NLLNKLERDNKEIRDNIYKAMSHVKPDYLLPQKK"
     gene            41006..41482
                     /gene="smpB"
                     /locus_tag="PLG98_03055"
     CDS             41006..41482
                     /gene="smpB"
                     /locus_tag="PLG98_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOE32259.1"
                     /translation="MAKEKTAQKLIASNKMARVNYEIGDIYEAGLVLTGTEVKSLRAG
                     KANLKDSYAVVKDDEVYIKEMHISPYDFGNRSNHNPLRTRKLLLHKREIKKLYGKSRE
                     KGLSLIPLKLYFKNGKVKVEIGIGKGKKLYDRRQDIKQREERRSLSRDFKSRQIKV"
     gene            41568..41921
                     /gene="ssrA"
                     /locus_tag="PLG98_03060"
     tmRNA           41568..41921
                     /gene="ssrA"
                     /locus_tag="PLG98_03060"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(41945..43414)
                     /locus_tag="PLG98_03065"
     CDS             complement(41945..43414)
                     /locus_tag="PLG98_03065"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOE32260.1"
                     /translation="MNIRISKTKYLNGLQCKKLLWYIYNEPDAIPAPDEAKQAIFDQG
                     HLVGDFAKRLFPNGAEVDHTGSFERGLKSTQEFLSQRVPIFEAALAYKNCYARADILE
                     PVGKDKWDLIEVKSSTDIKDINYPDIGFQYYVYSGAGLKINKCYLMCIDNKYVRKGDI
                     APKKIFKKIDITEEIKKTYSKMIEGNVEEMIKAIGSETYIEIGIGPHCSDPYECPLTA
                     QCWSFLPERNVCLLYSNNKLPMTLIRDGILELAKIPEDYELNKKHQIQVDCEKSGNPY
                     IDSKKLKEFLDTIQYPAYYMDFETCGSVIPPFDNSSPYQQIPFQFSVHVVQKKGAKPE
                     HFSFLADGTNDPRPEFMARLKDVMGTKGSVIAYNAGFETGILRKCAEVLPQYKNWVES
                     INERMIDLLQPFRAFAYYHPAQDGSCSIKNVLPAMTGKSYDDMEIGDGGMASSEYYRI
                     TFTEDNKDKDKVRKYLEEYCGQDTMGMVNIVNKLYSFTK"
     gene            complement(43460..43939)
                     /locus_tag="PLG98_03070"
     CDS             complement(43460..43939)
                     /locus_tag="PLG98_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32261.1"
                     /translation="MKLQELFDSSEYFKEIVNKQIPELNFDIEFVKYKKLFKDSFRES
                     DPPSATHKFSSGVYIFCDNKDNIIYIGKAANGNLAFRIWDHLKTPNDVNQFEKSPWVT
                     NHAYDKQARAIILKGNFTVSAIVVEPKELSSLLEVYLQTIHYIKEKKNLPVLNSQIG"
     gene            complement(43987..44589)
                     /locus_tag="PLG98_03075"
     CDS             complement(43987..44589)
                     /locus_tag="PLG98_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32262.1"
                     /translation="MGRYSIIIEDKNIYHALICRVVNIIKDKDLYNGRFTIKQLTSLE
                     IFSLEEEEYLKKIFSPDIDELDIWGEHYFYFIKNAWEYSEYIFYDYRKVIRMMMLLLN
                     KSKIPLCTKVRTDDYHLISKLWHMKILWESAALDADWENRECSVNYAVIELLSHNLKD
                     RLGDSCRFDFRFIKAFMEARTDYFRKKVNDAIMGIESINN"
     gene            44987..45229
                     /locus_tag="PLG98_03080"
     CDS             44987..45229
                     /locus_tag="PLG98_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOE32263.1"
                     /translation="MNTYDLIETKPEVMLGKPVIKGTRIPVDLIVRKLGEGASFEDLL
                     DGYPNLSREAIQAALIYAADMIRNETTIFLKTGTSN"
     gene            45226..45603
                     /locus_tag="PLG98_03085"
     CDS             45226..45603
                     /locus_tag="PLG98_03085"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOE32264.1"
                     /translation="MNTTKPLSFLADESCDFSVIRALRSVGYSVKAIAEISPSLPDEA
                     VLELAVAEKRLLITEDKDFGEWVFAHQHVMAGVLLIRYPSSTRSSMGAAVIDLIGGHA
                     PELDGSFTVLEPGRARIRTKIKD"
     gene            45929..46933
                     /locus_tag="PLG98_03090"
     CDS             45929..46933
                     /locus_tag="PLG98_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOE32265.1"
                     /translation="MPEKFDPYKSYGEKLIGLFVRLLFSGESHSLTDLARMLDCSKQT
                     VLRLIDNIRKAYDINIEETKQGNRSFYRIRKATASLQNIPLTEAELQSLQLCKAFTRH
                     LVGKKLYEEATQAILKSKTSLAAGHQVGECHFTAFRPGTIDYTPQHQIIHTLIDAMEK
                     HKVCKLTYQSMNSPRPKTYYIKPLKLFSHHDAIYLHAQKAKEPGKPYRAPQYDPLLAV
                     HRMKAIELTNISFQPDKFDFEKAFNQQFGIIKDEAFRVEAQFTGWAASFVLERIWSPD
                     QEIVKKGKNKILLKFTASSEPELISWLLSFGEQATLIKPDWLVKKLKLAIGGMEACYS
                     "
     gene            complement(46984..47265)
                     /locus_tag="PLG98_03095"
     CDS             complement(46984..47265)
                     /locus_tag="PLG98_03095"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE32266.1"
                     /translation="MKIYQSKLFEKKIKKMSKAEKTALDREVKNIAENPNVGEEKKGD
                     LKGVFVHKFKLKTNLYLLAYRKAGSDLELIMIGPHENYYRDLKGYLKSR"
     gene            complement(47272..47490)
                     /locus_tag="PLG98_03100"
     CDS             complement(47272..47490)
                     /locus_tag="PLG98_03100"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32267.1"
                     /translation="MATAVRISAELVDEAKRFSRIDHRSLAGQIEHWARMGKCAEENP
                     DLSYSLIKEILLGLDELDQGDKTEYRFG"
BASE COUNT        12751 a        10761 c        12008 g        12218 t
ORIGIN      
        1 tatggttcaa tatctattga ccagagaaaa ggtaattttt ccgatggaga aatttgttac
       61 acctaccgtc ccgctgcaac gaagattgcc aattatttgt tttcgctggc cgatgttttg
      121 gaacttaatt cttcagaaaa aatcaatcca tggtcaacaa tcgtgttacg tctcgaagag
      181 aaatcagccg ctacaggacc tttcagcggc caatatgtca atgatattaa aatttattta
      241 ggcgatactc aggggcgcgc aacaccgaca ggcaatccac tggatatgcc aagacatggc
      301 ggaaagcgct ggagcaatcc gccggttgcc ggggatgtcc agtggccggc tgaagccgga
      361 tggtccacgt cttcggcagc atcagcttgt tgctcggatg aaaatataaa caaggattat
      421 tttacccttg tccggggatg ggtttttaat acagctgcat ccgatgttaa tgctaattac
      481 gcgctaaccg gcacggcgga agagtccaat tcgacgattc gtaccataga gttcacgacc
      541 cacagtatgg gtacatttaa tcaaaatgaa ttggggttgt ggacatcggg taatagttcg
      601 gctaataatg tttattttga tgattttgcg gtgaggcttg aagaggctcg atctgtcgta
      661 ggatttacaa cgcccttaat ggaataattg ttaattttgc atctcaacat aaggctgaca
      721 tctgaaatta tcaggtgtca gcctttttta tgccttagat taagaagcat ttttgagaat
      781 atgcttaaca tttgactttt tcagtttata tgctatcgcc cgtcgccctg caaaatgaaa
      841 gagattttta atataaattc aaaggatatt gaaaagagag tataaatccg gtctgtgctt
      901 gacatttact gtattttatt taagaataca ggcatacaaa gattaataga atgtaaggaa
      961 aatcatggaa aaaatcatta aaattcatgt caagaaattg ccggaagggg tttatttggc
     1021 aacgtccaaa gatgttcccg gtttggttgc tcagggacga accgccacgg aaacactgga
     1081 gattgcccgt gatgttgcca aaaaattact ggaagccaga aaagaacgcc gtggcaagat
     1141 aaaaatatcc gagattaaag actcatttga ttatccgatg attgtgagcg cgtaacgtgg
     1201 gacgattggc gggtttccgg tatcgtgaaa tcgtaaagcg tcttaaatcg tttggctttg
     1261 cttttgacag gcaagcggcg ggaagtcatg aaatctggta caattcattg aacaatcttt
     1321 atacaaccat acccaatcat cccggagata taccggaagg aacgctgaga gccatattaa
     1381 agcaagctgg tattaccccg aatgatttct taaagaacaa ataaggcaca tttccccttt
     1441 tgatgcaggg cgagatctaa cctaaatacc ctaaagggca ctatagggtc atattgtccg
     1501 atagcgccga cttgccatat cggactgatc gggggatcag tccctacatt tattgtgaga
     1561 ctcaaatttc aaaaacgtag tgcattgaaa tttgtaagtc gaacaatccc gcgcaacgga
     1621 gggtttaata ccccgcagct tgctgcggaa tagtttccaa agcttgcttt ggggttcata
     1681 cccgtgattc ctaaacatga attctgctta atatttgact ttttcagttt atatgctatc
     1741 gtccgtcaca cattgttaca acgaagcaat tcttagcagg agggtcttga attgaacgtt
     1801 tttgtcaaaa aaggaatgct tgcggaaacc aaaagccagg cagtgattct gacgttgttt
     1861 gaagacggca agaaattatc gggcattgcg ctggagattg ataaaaaaag cggcggtctt
     1921 atcagcaaat taatggccga cggtgatttt gaaggcaagg cgtcgcaggt agccgttgct
     1981 tatccctgcg ggaatcttcc gtcgaagaga atcgctctgg tcggtttggg aaagcaaaag
     2041 gaattcaatc tcgaaaaatt gcgcgcggca tttgccaaag tgatgcgtca tcttcgcggg
     2101 atgaatgtca aggaagcgtc gacgtccgtg aatttgaatc ttattcccgg caagaaagat
     2161 cagatcgtcc aggcagcggt ggagggggct ttgctcgggc tgtatcaata taccccgtat
     2221 aaaaccgtcg gacgggaaga attcaaggag atggatgaat tcaatatcat cgccgatgcg
     2281 aaagagtatg ccgtgattga gccgtctgtg aaaaaagcca aaattattgc cgacgcggtt
     2341 tgctttgtgc gcgatatgat ttctgcgccc tccaacgaaa tgacgccttc gatcatggca
     2401 caaaaagcac aggaagtggc caagagaaaa aatgtgacgt gtacggttct ggatagaaag
     2461 aagatgcagg aattgggtat gaatgctctg ctggcggtgg catccggaag ccaagaagaa
     2521 ccgaagttta ttattctgga atattccggc ggcagaaaaa aagacgctcc cgttgtgctg
     2581 gtgggcaaag gactgacgtt cgacagcggg ggaatctccc tcaaaccgtc agataaaatg
     2641 gaggagatga agacggatat gtcgggcggc gccgcggtga tgggagcgat tatggcggct
     2701 gcggatatgc agattcctct caatgttgtt gcattgattc ctgcgtcgga caatatgccg
     2761 agcggatccg cgttcaagcc gggcgatatt ttaaaatcgt attccggcaa gaccatagaa
     2821 attctcagta cggacgccga gggacggctg cttctggccg atgcgctggc ctatgcttct
     2881 aagttcaaac cggaagccgt catagacctc gccacgctga cgggcgcctg tgtgatcgcg
     2941 ctgggcgacg atgtcatcgg aatgctggga acgggtgaaa agttgaaaaa agaaatcaat
     3001 cgcgccgcgc aagacaccgg agagcttgtt tgggaactgc cgctgtggga gcaataccat
     3061 gagctgatca agagcgatat cgccgattac aagaatgccg gcaaccgtgc cgcgggcacc
     3121 attacggccg ccgcattttt gagcaaattt gtcggcgatt atccctgggc gcatctggac
     3181 attgccgggc cggcctgggc ggacaaggat aaaccgtata ttcccaaagg cgcgtcaggg
     3241 atcggtgtcc ggttactggt agaatttctg cgcaaccgtt cctgatatca gtctatgccg
     3301 ccactgctgc aaataaaaga tctcagaacc gtgtttgata ccgttcatgg ccgcataaaa
     3361 gccgtggacg gggtatctct ggacatcgcg tccggggaaa ccctgggcgt ggtcggcgaa
     3421 tccggctgcg gcaaaacaat gctttccctt tccattatgc gtctcattcc tcccaacggc
     3481 aaaattatca acggtgaaat tctattttcg ggcgtggatt tgctgaaatt atccgaagaa
     3541 gacatgcgcg cccgccgcgg caaggatatt gccatgatct ttcaggaacc gatgacgtcg
     3601 ctgaatccgg ttttccgcgt cggcgatcag atagccgagg cgatccgtct gcatcagcag
     3661 ctgccggcga aacaggccat ggcgttgagc gtggagcttt tgcgagaagt gggaattgca
     3721 gagccgcaga aacgggcgcg ggattatccg cacaatctca gcggcggcat gcgtcagcgc
     3781 gtcatgatcg ccatagcgat gtcctgccat ccgcaactgc ttctggcgga cgagccgacc
     3841 acggcgctgg atgtcaccat tcaggcgcag attctggatc tgatttccaa tctgaaacag
     3901 aaaaataata tggcggtgct tttcattacg cacgatctgg gcgtcgtcgc tcaggcggcc
     3961 gagaaagtgg ctgtcatgta cgcgggaaaa attgtcgaat attccaccgt ggaaaatctc
     4021 tttgcaaagc ccttgcatcc ctatacgcag gggctgctgg aatcgatccc ggcgagatgt
     4081 tccgatgcaa aggaaaaaca ggcgtatcta aaaacgattc ccggctccgt tcccgatctc
     4141 tatcgtatgg cccggagttg ccgtttccat gaccggtgcc cctttgcttt tgctgaatgc
     4201 gaattgcagg atcccccatt gcttgccgtg gaagacggtc atctggtttc atgctggaaa
     4261 tacggatcgg aaaaaaataa ttaatgaaaa gagcaaataa tgtccagcgt tcttgtcgac
     4321 attcagaagg tagataaaaa atacacgtta ggcggcaatg tgtttggcgc tcaaaagcga
     4381 atcattcacg ccgtcaagaa cgtcgatttg cagattattc ggggggaaac gctggggttg
     4441 gtcggcgaat cgggttgcgg taaatcaacg ctggcccgtc tgatcaccag gctggagaat
     4501 cccacatcgg gcgtcatcgc atataaaggt gaaaatattt ttacgttcac gggcgagtct
     4561 ttaaagacct atcgccgcag cgtgcagatc atttttcagg atacgtattc ctcactgaat
     4621 ccgcgaaagt cggctttaag catgatccgg gagcctttga ccattcatcg cctgggaagc
     4681 aaaaaagagc gcgaggaaac ggcgctgtcg attatggcga aagtcgggtt gaaaaaggaa
     4741 caggcccatc gctacccgca tgaattcagc ggcgggcagc ggcagcgtat cggcattgcc
     4801 cgtgcgcttg tattgaatcc ggaattgatt attgccgatg aaccggtttc ctcgctggat
     4861 gtttccattc aggcgcagat tctgaatctg cttcgggatt tgaaaaacga ttttcatttg
     4921 acgtaccttt ttgtttccca tgatctcaac gtcatccggt atatttccga ccgggtggcc
     4981 gtgatgtacc tggggaagat tgtcgaactc gcgggcggcg aagatattta caaccgtccg
     5041 ctgcatcctt atacacgcat gctgctttcg gccgtgccgt cctgcgaccc gggtaaaaga
     5101 aaaacgtcca ttgccataaa gggcgaagcg agggagacgg ataacgaagg gtgcgctttc
     5161 cggaaccgct gtccctacag aatagacgtc tgcgataaga cggatcctgc tctggacgcc
     5221 ataaacggcc aaagcttttg tgcctgccac agggccggtg caatttaaaa tagcctttgt
     5281 ttcctttctg tcatttcgtg tatcatggcg gaaagttcaa tttcaaaaag gtctccgtgt
     5341 gttatgcggg aagaaaagat cctgataaag aataatgctc tggtgattga agccttgctg
     5401 catcgggcat cgggcgaacg aggtgcggtg atctgccacc cgcattcgct gatgggcggt
     5461 tcgatgtata ataatgtcgt tgaagcgctg caggaggcgt ttgcatcgga agggatttcc
     5521 accttgagat tcaactttcg gggcgtgggc tccagcaccg ggtcgtatag cgaaggcgtg
     5581 ggagagaagg aagatatttg ggctgccggc aactatctga aaacgtcagg tctgcgcgag
     5641 atattttttg cggggtattc gttcggagcc tgggtcggtg ccggggtgat tgcgcaaccg
     5701 gatcatcctt tttgcagcag cgtctttgta gcgcctccga tagattattt tgaatttgac
     5761 tggccgcctt taaaaaataa aattgattta atgatctgcg gcgatgccga ttctttctgt
     5821 ccgtgggagc ctttgaagga tgcgtcaaaa aaaataaatg cgttgctgga agtcattggc
     5881 ggcgcagatc atttttttat ggggaaagaa aaaaaacttg ttattctttt gcgggattat
     5941 ctggctaaaa aaacataaat taaataaaaa aacttgattt caaaaaaaaa agatgataat
     6001 atttcataca acgcataaaa aaccgaatat aagaaacacg tcaaagatag gatgagaacg
     6061 gtttttttta ggaagttgtt agtgttaagt tgtataataa ataagacaac cgcggaggat
     6121 tcttaaaaaa tatagaattc ttgcggtttt ttttgtttcc tgtacaaaca ttaaaacagg
     6181 aaatcatttt ttaaaggagg gttttagctt tggcagaagg aaaagtaaag tggttcaacg
     6241 agaagaaggg gtttggtttt attgaaaatg atgaaggtgg tgacgtgttt gtacattata
     6301 gtgcgattac cggaacggga tttaagaccc tttatgaagg tcagaaaatc aagttcgata
     6361 ttgaacaggg gaataaaggt cccagcgcaa caaatgttac gccactgtaa tactcgcaat
     6421 tgatttattc taactgaaaa aatgccccgt gatgaacggg gtattttttt tcaacgattc
     6481 cggatcttaa gcagccgcat tggatcacag gaccgggcgt gaagtatgcc gtcttgtata
     6541 tcaaaaaata tttaacgggt ttcaagtttc cgaagctctt tttctcccca gtaaataaca
     6601 agaaataatt caatgaagcc ggccaggggc aatgccatcc aggctccggt gattccccag
     6661 aataaaggca ggataatcag taacggaaca aagaccagaa tatcccggat gaaaagaata
     6721 atcatggcca gcgtcccttt gccaagtcca atgaacatgt tgacgtaaat gatgatggga
     6781 gcggaaaaga tcagggctgc gacatggatg cgtaacgccg gtatggcaat ggcggttaaa
     6841 tggggatcgc tggaaaaaaa agtgatgatc tgggaagaaa acaattccag gatgatcacg
     6901 gaaaccgcgg aaaaaacaac agagactttc aaggcgacgg agaccgattc ccgtaatcgt
     6961 tgaaagagcc tcgcgcccag gttatatccg acaatcggca ttaatcccgc gccgacgccg
     7021 aacagtacca tggtggccag gccggtaacc cggaaacaaa tgccgaaggc cgcgatggcc
     7081 tgaaaaccga aattgccgag aacatgattg tagatcacca gaacaatgct gatgacaaaa
     7141 ttggtgacca tcgaggggaa accggttgaa taaatggact tgatgatctg cagatcggga
     7201 cgggtgtatt tcagtctgaa atcatacttg gatgatttca attgcaaaaa ataaagggac
     7261 aagataaaca tgatcaagtg cgcgatgata ccggccagcg cagcgcctcg gattcccatg
     7321 cgtggaaagg ggccgatgcc gaaaatcaga aaaggatcga ggatcgcgct ggaaatcagg
     7381 gcaatcagga caacgttcat ggataaaaga ggacggcctt cgacacgcag catgatggcc
     7441 gtcatcatgg aaaaaaagag aaaaggcgag ctgtaaacgg ccagaaaaag ataatcatga
     7501 ctcagaggta atatttcgtc ggacgcgccg aaaaagacga gcatggaatc cccgaaaaga
     7561 agcacgaaaa gaatgcttcc gaagccgaac agcatggaaa gaaaaaatgt ctgcccggcg
     7621 gttttctgtg cttccagatt tttttcggcg ccaaacatcc ttgcggaaaa tgaaccggct
     7681 ccgatgccgg ttccgacgcc tatcgcggcg gcaagcagtt gaatgggaaa gcacacggtc
     7741 agtgcggcaa gtgcctgaga gctgaaacgg gcaagccaga aggtgtcgat gaaattgtaa
     7801 agagacatgg ctgtcatcgc aatgatcgac ggccagctca tttttaaaat cagcgggagg
     7861 attgcctctt ttcctaaatc cagtctctta ttcattatgg gtcatttcat ttgaatgcgt
     7921 ttagttgcta gcatataaag aattagaata tcaagataaa atatggcaaa aaatcttatt
     7981 gttatcctcg gtccaacggc atccggcaaa acccgcctgg cggcacaact ggcctgtgat
     8041 tttcagggag aagtgatttc cgcggattcc cgccaggtgt acagaaccat gaacatcggc
     8101 accggaaaag atttaaagga atatgtgatt gacggccggc agattcctta tcatctgatt
     8161 gatattgaag acccggaaaa cgaatttaat gtattcgagt ttcagaaacg attttatgcg
     8221 gtgttcagcg ctatccgcca aagaaagaaa ctgccggtcc ttacgggcgg aacagggctg
     8281 tatctggaag cggttctttg cgcatatgat atgcccgaag cgccgatcaa tgaagaaatc
     8341 aggcgtgaat tatcagagaa atcgatcgaa gagcttcagg atcttctgtg ccgtttgaag
     8401 ccgcatctcc ataacagaac ggatcttgat gataaaaaac gactgctccg ggctgtcgaa
     8461 attgaaaaag cgcggattcg caataatcag gatttgaggg acaggcctga aattacagcg
     8521 gctgtcttcg gggtgcgatg ggaacggtcg gtcctcagaa accgcatcac aaaacggctt
     8581 gaagagcgac tggaaaacgg gatgattgaa gaagttgccg gtttgcatgc ggccggtttg
     8641 agctggctta ggctggaaag tttcggcctg gaataccgtt ttatttcgga atatctgcag
     8701 cagcggctta cgtttgatga aatgaaaaat cggttaaaca tcgcgattca tcagtttgcc
     8761 aaacgacagg agacatggtt ccggcgcatg gagagaaaag gtgttcaaat taactggatc
     8821 gataacggcg actacttgct tctggcagaa aaggttgcca agcatttaaa atgaagaagc
     8881 gttccgcatt cgatgattac ctgcacacgt actccgtcgg cgaaaaatgg aagttatgtg
     8941 ccgtcccgga tcaaaatttc tctcaggttg tcgtcattcc ggcgtacgcc gagaaagacc
     9001 tgcttttccg cacgctggct tctttagccg tcaatgtgcc tgatgcgctg gagaattctt
     9061 ttatcctgtg cgttgtcaat cacaaagaag attcccccgc ttccgtcatg gaaaacaata
     9121 aaataaccat ggcgtatttg aacgctctga tcagacggaa gtctctgaaa acgtttcagc
     9181 ttaccaaaga cgtcacggct cttttgcagt gcatatcgga ttcaaaaatg aagctgggat
     9241 atattgacgc tgcctcgcgg ggatatgaaa taccgcgcaa tacaggcggg gtcggcatgg
     9301 ccaggaaaat aggcatggac atggctttgc gccttttgga gaaaaatgcg ccgcaaaacg
     9361 gcgcgatcgt cagcctggat gccgataccc tggttgaacc gaattatctt tccgtcgtcc
     9421 gggattattt cagaaagaat gttaaaaccg cgattatggc ctatgcacac cagatgccgg
     9481 aaaatcccgc ggaacaggcg gccatttgct gctatgaaat tttcttgcgt tactgggttc
     9541 tggggctgcg gtatgcaaaa tctccctggg ctttccattc catcgggtcg accatggcgg
     9601 ttgccccgca atcgtatctg gccgtaaggg ggatgaacaa aagggcggcg ggagaggatt
     9661 tctactttct caacaagctg gcaaaagtcg gccgcatcga ctacatcagg gaaacgtgtg
     9721 ttcatccgtc ggcgcgcgta tcaacgcggg ttcccttcgg aacaggcaaa cgcatcgagc
     9781 gctttcttgc gggagacgcc gcaaaagaat attacctgta cgatccgcag atattttcaa
     9841 tattgtccgg ctggctcagt cttgtacatc ggatgattga ccgggacgtg gatgacatct
     9901 tgatggaggc taaacggatt catcccgcct taaaggcctt tctggaagag gcccggtttc
     9961 ctctgcgctg gtccgccata cggcgcaaca caaaagatca agagactctt ttgaaccatt
    10021 ttcatgtctg gttcgacggg ttcagaacgt taaaaatgat caattatttt tcccgggtgg
    10081 cttatcctcc ggtggatatg ttttcagcat tgaagcggat tttcaacctg ttagatctgc
    10141 ctgctttgag tccggaagtc gcagaagata ttccttcttt gggcgaacaa atggaaattc
    10201 tgcgctttct gagaacaatc acctgaacgt catttagtac cggaaaattc tcttgacgga
    10261 tacggataat ttacatataa cacgtgaaaa tctttaaaat ttgcaaaatt ttcgggagga
    10321 aattcatgaa aacttggatc ggctcaaaat ggcccataat ttttgttttg tttgtttttg
    10381 tcatggctgc cggttgttcg gatgccaaaa aaggtgcgga gaacgcagtt ggtgcaaaca
    10441 gtgttgaaag tttggatgca ggacagccct cgacggttac tgatgcggct gcccaaaaca
    10501 ttccggcagg cgatctcgcc gtcacggttg acggaaaaca tatgaccaag gccgaactgg
    10561 ataaggcggt caaagaaaaa ttcgagctta tcaaaaaaca ggtgccggcc gacaaacaaa
    10621 aagcattcag ggaaaatctc agaaagcagt tggtggacgt ctttgtcatg aagacacttc
    10681 tggccaacga agtggagaaa agaaaaatcg tagctactga tcaggatgtc aaagcggcca
    10741 tgaatcaaat caaagccagc cttccgccga ataaaaatgt cgacgacttt cttaaggaaa
    10801 ataaaatttc caaagaagac attattctgg ccgtcaagat cgataaattc agaaatgcgg
    10861 aagggggcaa ggcgctgaaa cccacggaaa aagacatcaa caagttttat aatgacaatc
    10921 gtgaaaaact atttgtcgaa ccggaaagcg tgcatgttcg ccacattctg gtatctgtcg
    10981 gcaaggacga cagcgagaaa gtcaaggcgg agaaaaaagc aaaaattgac gatctgcgca
    11041 aacaattggt cggcggtgca aattttgcgg aaatcgccgg tcagcattcc gattgcccca
    11101 gcaagcaagc cgggggcgat ttgaatttta tcaggagagg ccaaatggtg aaagcgttcg
    11161 aggatgccgc ttttgcgcag gagaaaaatg ccatcggtcc ggtcatcaag acggaatacg
    11221 gttaccacat cgttcaggtg ctggaccgca gaccggcgaa gaaaattgcc ttaagcgaag
    11281 tcaaaagcaa aattgccgat tatctggaaa gacagaaaaa aatggaagcc ttcaacaacc
    11341 tgatggccaa gctgaaggcg caggccaaaa taaccgttta ttaaacatca ttacaacgtt
    11401 ttacagagtt acttgaaact gtgttccgta tcggggaagt tgctgttctt gacttccccg
    11461 atatatgttt tgaccgcttt cttgatttcc agattgaggt tggcgtattt tttgacaaat
    11521 ttcggcgtga atttttcctg catgcccagc atatcgtgaa cgaccagcac ctgtccgtcg
    11581 caataaacac cggcgccgat gccgattgtc ggaatagaca gggcgctggt gatttcagca
    11641 gccagttttt ccggcacgca ttccagcaca acggcaaacg cgcccgattc ttccagaatc
    11701 ctggcatcct gcatgattgt ttcagcggcg tcgtcttttt tgccctggac tttatatcct
    11761 cccaactggt gaaccgattg cggtgtcaga cccagatgcg ccatgacggg aattcccgca
    11821 tacgtcattt tatgcacgat gtccgcaaat tcccggccgc cttccagttt cacagcctgg
    11881 gcatcggctt ccttcaggaa ccgtcccgca ttggcgagcg ccgcctcagg agatacctga
    11941 tacgataaaa aaggcatatc cgcgacgacc atcgccttct taaccgcacg cgcaacagct
    12001 ttggtatggt gaagcatgtc gtccatcgtg accggcaagg ttgaatcgta gccgagcatg
    12061 acgttcccca gagaatcgcc gaccagaagc atgtcaatat cgctttcatc cagaatagag
    12121 gccatggcgt aatcgtaagc cgtcagcatc gcaatttttt ctcccgttgt tttcatcttt
    12181 ctgatatcag cggttgtttt acgcgttatt ttactatgag tgctcatgct ttcctccttt
    12241 taaaagggcg ttgatttttc ttgcctgtcc ggcgttgagg gttcctttct gttgcgcaat
    12301 cttgacggtt atcaatccca gagaagcata taacgaagaa taccggggaa gatttttatc
    12361 aatggtccgc agatgtttcc ggatggtgcc gtaatctccg cgtgcgatgg gaccggtcag
    12421 ggaggatatg gaaccggatt tctcgatgtt tttcaaactg ccgtatacca ggggcaagta
    12481 agctctttgt gcgtcttttt ccctgatgcc tatggatcgg taaatcgatt ccacaatgtt
    12541 catcagcgat acaagataat tggaagcgaa acaggcggcc gcgtgataga gcggtttctg
    12601 atcgtttgaa atgaccatgg ggatgccgcc cagtttatga atgatatgtt tggccggaat
    12661 taaggaatat ctgtcggcgg taacgccgaa gtagcttccg ggaatgtttt tgatggcctg
    12721 gtcgatggat gaaaagcttt gtaacggatg aatgcttgcc acaaaagcac cggctttttt
    12781 tgacgattcc agaaggtcaa ggccgccggc gccgctcatg tgaaacacat attttcccgc
    12841 gatggatgac gcggaggcga tttcccggca ggccgaggca atatgatcat ccggggttgt
    12901 aatcagaata gaatcggcgt gtccaacggc atccggaatc gttttgcacg gttttcctcc
    12961 ggtataggaa acggctcttt taagggcggt cgaagatttg tcgcagatcg aaacgacgtt
    13021 gtaaccggat tttttcaaca gagagcctat tgcggtgccg accatacccg tgccgatgat
    13081 ggcaaagtta tttaactctt ttttcttcat gtgatcacgc atcgctgtgc atctgactaa
    13141 ctgaaaccat atccctgccg cgtcagaggc agggacaaac atcgctgcgc tcgttttcag
    13201 caagtctcaa atcccacgtc gctacgctcc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnggataatt cgctctaaga ttttccgtgc actacgtttc ggaaaatcaa
    13381 ggctcatgac gacttacaaa tttcaatgcg ctatgctttt gaaatttgag tctcaaatcc
    13441 cacgtcgcta cgctcctggg ataattcgct ctaagatttt ccgtgcacta cgtttcggaa
    13501 aatcaaggct cattaaaaaa ggccttccgc aagggaagac ctttatccga catttcagtt
    13561 caataccgtc tcagtcgata agagagatcc aagcggttag aacttatcta acaccgaccc
    13621 aaagcttagt caaacacttt ttcataaaga atcttgtgtt tacggacgca cttggttata
    13681 taacttaaca aatgcgaaga ggttcatgat gaagaaaata gatctgatga tattcgattt
    13741 ggacggtacc attgcctcga cgggcgatga cctggttttg tgcatcaacc ataccttaaa
    13801 aaccctgaac ctcagggaaa gaacgaaaaa ggacattatc ggctttgtcg gcgacggaat
    13861 cagcaaatta atggagcggg ctttgggaag ccgctatcgc gaataccagg aagaggccat
    13921 gcggatattt tccgcgtatt acgcggagca tcttctggat aatacgaaac tgtatcccca
    13981 tgtggaagag gttttacgga attttgagaa caaaccgaaa gtcgttctga ccaacaaacg
    14041 gtatccttat gcgttgatga ttgtcagagg gctgaagata gaaaaatatt tcacggaagt
    14101 catcggcgcc gattcaactc cttttttaaa accggatcgt cgtattatcg atagcgttct
    14161 ggaaaaacat ccggccgaac ggcacagagc cctcatcgtt ggcgacggcg tcaacgatat
    14221 tgcggtcgcc agaaattccg gaataatgag ttgcgctttt ttaaacggat tgggccgtcg
    14281 cgacgatttg ctcaaagcga aggccgatta ttactgtgaa gacattcggg aagtgaatcg
    14341 gctttttaga tagttatgat caaaaaagtg aaacagacca tcgagaagtt tgatctgctg
    14401 aaaaaaggag agcgggttgt cgttgccgtt tccggcggac ctgattcgac cgcttgcctg
    14461 accgttctgg cttcgatcgc cggggacatg gacctggaac tcatcgttgc gcatttcaat
    14521 cacggcttga gaggaaagga atccgaccgg gatgaacagt ttgcccgccg cctggctaaa
    14581 aagctggggc ttgttttcta tgcgggaaaa ctgaataaaa agattcgtgt caaaggcatt
    14641 tcgccggaag atttttaccg gcgtcagcga tatgccttcc tggagaaaac ggcggcaaag
    14701 caccgggcgc agaaaattgc gctggggcag catttaaatg atcaggcgga aacggttttg
    14761 ctgaatttat tgcggggcag cggtttggaa gggctcaaag ggtttctgcc catcagggag
    14821 gggcggatca ttcgtccgct gattgaagta tccgcacagg aaatcatttc ttttctggag
    14881 cagtcgggca tggcctattg ccgggatagt tccaatgaaa gcagtcaata tctacgcaat
    14941 cagatccggg cagagctgat tcctttttta aaagggaaat acaatccgcg aatcgaagag
    15001 aatctggcgc aaatggctga aattcttcgc cgggaagatg attttatccg tcagcatgcg
    15061 gccgcagccg caaaaaaaac catgcagcag aaaaaggccg gttctgtggc gttgaacatc
    15121 catgcggtta aaaaaattcc cgaagccatc cggtggcgat tgctgaagat gcttctggag
    15181 gatctgacgc cttcaggcga cggcatatcg tttcttcatg tgaatgcggt gaatgatctg
    15241 acgcagaaaa acgcatccgg aaaaaagatc acgcttccgg ccggaatcga agcgagacgc
    15301 gaatacgatc agttaatcct ggagaagaaa aggacgcgtc caaaaaaaaa tcaatacgcc
    15361 tatgtgatga gcgttccggg aacggtccat gtgaaagaga gggggctgac gattaaggcg
    15421 acactggtta aaaaaattaa gacggactta tcccgcatga aaaatacggc gtgtctggat
    15481 tggagtaaaa tcgctccccc gctggtggtc agaaacagac gcgacggaga ctggatccag
    15541 cctttgggga tgcgcggccg gcagaaaatt aaaaaatatt tcatcgatca taagattccc
    15601 gttgctcaaa gagacgacct cctgctgctg gttgaccgtc tttccgtcgt ctatattgag
    15661 cataggcata tcagtgaccg tgtcaagatt tcaccggcaa caaaatcggt cctgaaactg
    15721 gcgatcacgc ctgaccggcc tgtcgggaaa ggttcaaagc cgcccggata aaatccatag
    15781 aaattatttg agataaattc aatattatta taagagtaaa ataactgttc taaaggagat
    15841 taaatatatt gaatcagttt cagaaaaata tagcgcttgt cctgaccgtc ctgctggttt
    15901 tcctgcttgt ctggcagctt tacaatcagc ctaaaacggg aaccaaggaa atcatataca
    15961 gtgagatgtt aaccctgttg gacaagggag agatcagcga agtcaccatt cagggcgatc
    16021 atatcaacgg aaaattgaaa aacaacagtc tttttaagtc gtttgcgccc aaagacgacc
    16081 aactggttga aaagttcagc gggaaaggcg tcaagataac tgccaaaccg gtggaggaat
    16141 ccgcctggct gaccattttg atttcatggg cgccgatgat tctgctgatc ggtatctgga
    16201 tcttttttat gcgtcagatg caggccgggg gcggaaaggc catggctttc ggtaaaagca
    16261 aggcgcgcct gatcaccgat aaatccaagc gggttacgtt tgccgatgtg gccggaattg
    16321 atgaggcgaa atccgaattg gaagaagtga ttgatttcct gaaggatccc aaaaaataca
    16381 ccaagctcgg cggcaggatt cccaagggat tgttgctggt cggccctccc ggaacgggaa
    16441 aaacgcttct ggcccgggcc attgccggag aggcggaagt gccgtttttg accattagcg
    16501 gctcggattt tgttgaaatg tttgttggcg tcggcgcctc acgtgttcgc gatttgttca
    16561 gtcaggccaa aaaaaacgct ccctgtatta tttttatcga tgaaatcgac gcggtcggcc
    16621 gccatcgcgg cgccggtctg ggaggcggcc acgacgaaag ggaacagacg ctcaaccagc
    16681 tgctggtgga aatggacggc ttcgaatcca atgaaggcgt tatcttgatt tcagccacca
    16741 accgccccga tgtcctcgat ccggcgttgc tgcggccggg acgttttgac cggcaggttg
    16801 tcgtgccgct gcccgacgtc agaggccggg aaaaaatctt cgaggtgcac gcccgcaaga
    16861 ccccgattgc gggagatgtg gattttgccg ttctggcccg gggaacgccc ggcacgtccg
    16921 gagccgatat tgaaaatctg atcaacgaag cggtgctcaa cgccgcccgg gccaataaag
    16981 aaaaggtgaa catgagcgat ttcgagtttg ccaaagacaa gatcctgatg ggctcggaaa
    17041 gaaaaaccat ggtgatcagc gatgaagaga aacgcaatac ctcgtatcat gagatcggcc
    17101 atacgctggt cgcccgtctg attccgggaa cggatcccat tcataaagtg accatcattc
    17161 cccgcggtcg cgcgctggga ctgacccagc agctgcccgt ggatgaaaag catacctatc
    17221 cgagacggta tctggaaaac agcatttcca ttttgctggg cggcagagct gccgaggaac
    17281 tggtcttgaa agacttcacc accggcgccg gcaacgacat tgaacgcgcg acaaatttgg
    17341 cccggaaaat ggtctgtgaa tggggcatga gtgacaaaat ggggccgttg agttacggca
    17401 aaaaagaaga gcagatcttt ctgggacggg aatttgccac gcacaaggat tatagcgaag
    17461 aaacggcaaa aaacatcgat gcggaagtca tggccattgt agcgcgcaat tatgaacgat
    17521 caaaacagct tctcaccgat catattgatc ttctccacaa gatatcgggt gaacttctgg
    17581 aaaaagaagt tttaagcggc gcggaaatag acaccctgat cggcgatgcg ttacccaagg
    17641 cacacaggag cgagccgcag acttcagtct gatcactata tcacttcaac catccgttca
    17701 taatattggg aatcttgaaa tttggtgatt tctggtgaag ataattgata ttcaaagcac
    17761 acaagaggcg gctgcgctgt tcaggcggat cggggtggat gcctacggca tcggcgcgat
    17821 ggcgcccaaa gcggtcaatc tgaatatcct gctggagaaa cagccgtgta aaattgccaa
    17881 tatcctgaag caggaaatgc tggctctcgg cggtgatgcg gcggttgcca gaggaagtgt
    17941 cgcctgcagc gtggaaacga ccgatgtttt aatgatgggg acgctgaaac agctctcccg
    18001 gctggcggcg aagatagccc ggcagccttt tggtctggat agcatcgccc gggatatttt
    18061 gcggattctg aaaaacgccg ggcagaaacg gtttatctta aaaacatccc ggaggaaaat
    18121 cacgctgggt gacagaaccc ggattatggg aattctgaac gtcaccccgg attcgttttc
    18181 cgacggcggg acctttctat cagcggaaag ggcggttgaa tatggattgc agatggaggc
    18241 ggaaggagcg gatatccttg atgtcggcgg agaatcaacg cgtcccggtt ctgccgcagt
    18301 accggttcat gtcgagctca aaagagtcct tcctgtgatc aaagcgttga gccggaaaat
    18361 aaagattccc ctctccatcg acacgaaaaa agcgcgtgtt gcccgggaag ctgtcgaggc
    18421 cggggcggaa atcatcaacg acatcagcgc cctgaacggc gatcgtaaaa tggccgggac
    18481 ggctgcggaa acgggcgccg ctcttgtgtt gatgcacatg cggggcaatc cggaaaatat
    18541 gcagaaggga aatctggcct atgatgatct gctgggagac atcacggatt atttgagcgc
    18601 cagtatcggg aaagccatcc gggcaggggt gagcaaagac gcaatggtga ttgatccggg
    18661 aatcggtttc ggcaaaacgg ttgcggataa ctacagaatc atcaggaggc ttgcagacct
    18721 gagaactctg gggatgccga tactggttgg accatcgcgc aagtccttta tcgggaatgt
    18781 gacaggggat gaaccgcggc aaaggctgga aggcaccgca gcgacagttt ccgcggcgat
    18841 tatgaacgga tgtcacatcg tccgggtgca cgacgtggcg gccatgaaaa aagtggctgc
    18901 ggtgacggac gcgatgacac atccccggaa ggtgatctga tggtttacgg aataggggtc
    18961 gatcttgtcg aaaacagacg catggaaaga atcatcgatc gatggggtgt caagttctta
    19021 aacagaatct tttccaccga ggaaatggcc tactgcggca aacatgctta tccggtgacg
    19081 cactacagcg cgcggtttgc cgccaaagag tcattcctga aagcgctggg aataggtttg
    19141 ggaatgggca taaaactaag cgacatttcc gtcgtgaatc atgaaggcgg ccagccgttt
    19201 ctgaatctct ccggcgatgc tcaatcctac attcaaaaaa gaaggatcca caagattcat
    19261 ttgagtctga cgcatacaaa aaaatattcc gcagccatgg ttttactgga aacaatcgat
    19321 taaaagccgg tgaaatgaca agatgagtca ggatgataaa acggttgaga taatatccgt
    19381 aagtactgaa aatacaatgg aaatagggcg ctctatcgga gaagggctcc aggatggaga
    19441 cgtggtcgcg ctttcggggg agctgggttc gggaaaaacc tgttttaccg gggggctggc
    19501 ccgcggtttg ggcgtggatg aaaaatacag gatcaccagc ccgacgttca cattgattaa
    19561 tgaatatccg gcgcgtcata ggctttatca tttcgacgtt taccggatca gcagttattc
    19621 tgaactcgac gatctgggat gcgaagaata tcttggaaca gaaggcgttg ttgttataga
    19681 atgggccgag aagatcaaaa agattctgcc ggaaaaaacg gtttacatcg attttgaata
    19741 cgttgatgaa aaccggagaa aaatgatcat cagggcgtcc gcagacaggt taaacgagtt
    19801 gatcaaagat ataaaaacgg agggtgttta aatatggcat taattgttca gaagttcggc
    19861 ggaacatcag tcgcgaatat tgagaaaata aaaaacgtgg ctgaaaaagt gatcaaggtc
    19921 aaacaggccg gccatcaggt tgtcgttgtt ttgtccgcca tggcggggga gaccgacagg
    19981 ctgattaacc tggctcacag tgcgacggat ttgcccgatg aaagagaata tgactccctg
    20041 atttccaccg gcgaacaggt gaccgtaacc cttctggcca tcgttttgaa tgcgatgggc
    20101 tgcaaggcca gatcgtttct gggctttcag gtgaaaatat taaccgatca ggcttataag
    20161 aaagccagaa tttcgctgat cgatacggaa aacatcaaaa aggaacttaa aaaaggaacc
    20221 gccatcgtga ttgcggggtt tcagggggtg gataaagaca ataacattac cacgctggga
    20281 cgcggcgggt ccgataccag cgccgtggcg ctggcagcgg cattgagagc ggatcagtgc
    20341 gacatttata cggatgttga cggtgtttat accaccgacc cgaacgtttg caacaaagcc
    20401 agacggctgg aaaagatttc ctacgatgaa atgctggaga tggcgatgat gggtgccaaa
    20461 gtcctgcagc cgcggtcggt ggaaatggct aaaaaataca atgtgccggt ttatgtcaaa
    20521 tcaacatttt cggataaagg agggacgctt gtgaccaggg aggataaaga tatggaaaga
    20581 gaaatcattt cgggtgtgac gtatgatcgg gatcaggtca agattacggt tgtgcatgtg
    20641 ccggatcagc cgggagtggc ggcgtctttg ttcagcccgt tgtcggataa aaatattttt
    20701 gtggatatga ttattcagaa tgccagttcg gaaggctata cggatctgac tttcaccgta
    20761 tccaaaaagg atttgaagga agcgcagaaa attgttgaaa gcacggcgaa aaaagtcgga
    20821 gcgaagaaaa ttgaagtcga tgatcaggtt gccaaaatat ccattatcgg cgtcggcatg
    20881 gccagtcatt ccggcgtggc cgcgaagatg ttcaaaacgc tggcggctga aggcgtgaat
    20941 attatgatga tcagcacatc ggaaatcaag atatcctgca tcattcaacg caaatacatg
    21001 gaactggcgg tgtcggtgct gcacgatgct ttcggactgg ataagaaaaa ataacattgt
    21061 atttatttca gagagcagac tgaaggggct gatcgttttg agccttatgg tggcggtcat
    21121 cccctttgtt tctctgatga tcacctcttg gactccttac cggattcccg tttatgccga
    21181 cacgtgcgat cactgctgtg tgatcgagat cgtcggcgat gatcaaaggg ccggaattta
    21241 ccacgttcca tcggggatgt cggtcaatcg cctgctgaaa accgcgggcg tcgaaaagca
    21301 attcgaaaag gactttaccg tcaaaaccgg gatgaagctg gtgattcgtt ccgcatcgga
    21361 ccgtccgggt attacggtgg cggaaataca aaacacagcg aaaatctctg tcggattacc
    21421 catcgatgtt aataaagccg gggaagaaga tttgattctt atcaagggca tcggaccggt
    21481 gacggcgaaa agaattctgg aattgcggac gcggctcaac ggaatcagcg acatccggca
    21541 gttgatggag atcaagggca tcaaggaaaa gagaatgaaa catattgaga aatatctgta
    21601 cgtggaaaag aaaaacgtct gaatcagttg ccgctgaacg atctgtaaaa actcaggacg
    21661 cgtctgacat aattttgtgt ttcccggtaa ggcggaatgt tgtagttgta tttggccacg
    21721 gcgccttcgc cggcattata ggcggcgaga gccagcgaca gattgccttt gtataaattg
    21781 agaaggtatc gaaggtaccg cgcgcctcct tcgatgtttt tttcaggatg aaaactgtca
    21841 tccacattca gttgcgatgc ggttttcggc atgagctgca tcaggccctg agcgcctacg
    21901 ggggacacgg cccggtgatt gaaattcgat tccgccttga tgattgcttt gattaaagcg
    21961 gagtccagtt tgaatttatc ggcggtttta ttgatgatgt ggtcgtattt attaacgtcg
    22021 agctggggct gaaacagaat gcgtttttcc ctcatgacca taacgtattt tgcgccggta
    22081 tcggaaggaa cattggtcag atgcaacacg ccttcattat cttcgtattt gtagatatcg
    22141 gcacgggcta tgtccgtgaa cgcggcagcg aaaaaaaata acagtgccgc gatgacgatt
    22201 ttggaaaata atgttatatg aagtttgaaa agccggtaaa tattattttg catttgctta
    22261 taatacttta tcatattgga aagttcaata tttttttgat cacgcacaca ccggtgcttg
    22321 atccctccgt ataaaccgga tgctttcggg taaacagcgt gaaaaaatcg agatggcctc
    22381 aaatggtcat gccgccgtct atggcgagca cctgccctgt gatgtagtcg gatgcatcgg
    22441 atgccagaaa gatgacggcc ccgaccatat cgtccggttc tgccggacgc cccagcggtg
    22501 ttctgctaat ggcgatattc aggatatcct gattggtcca taacggttcg ctgaactttg
    22561 ttttggtcag actgggtgcg atggcattgg cccggatgtt gtagatcgcc cattgttgcg
    22621 ccatgacttt cgtggccata atgacgcccg ccttgctgat ggaataaacc ggcaggatat
    22681 cgggagaaag accggcaacc gatgctacgt tgataatacg tccgcctcct ttttccttca
    22741 tgatacgggc caccgcctgg ctcaagaaaa acaacccctt gaggttcaga ttcatgatgg
    22801 aatcccaggc gcggtcgtca atatccatcg ccggcaccat ggtcggattg gttgcggcat
    22861 tgttgaccag aatatcgatt ctgccgaatt cggcagcggc tttgtcgaca agatttttta
    22921 tttcttccag acggccgata tgcgttgcta ccggaagaca ctttcttccc agcgatttta
    22981 tttctccggc cacgttttcc agatcgccga ttttgcggct ggcgatcacc acatccgctc
    23041 cggatttggc cagtcccaac gcgattgctc tgccgatgcc tctgctgccg ccggtgacca
    23101 gcgccacttt attttccaac gataattgca tggtgttgcc tcctgtgaaa ataatgatta
    23161 ttgtgccggc atatttttgc ggctgttcat ttgacgaaag acgtcccttt ccttgtcata
    23221 catgattttt gcctctttcc gggacgtatt ttcatgattg taacccagca gatgcaaaat
    23281 gccgtggatt aaaagaaaat caatttcttc ttccaacatc aactttgccc ggatggcgtc
    23341 ccgccgggcg gtttccacgg aaatcaccac atcgcccagg atctggggat tgatatgtcc
    23401 atactcgcct tcgcgcatgg aaaaggaaat gacattggtc gccttatccc ttcccagata
    23461 gcacctgttt atttcccgga tttgttcatc atccgtaaaa aggatgctga tgtgccggtc
    23521 cgtacattcg aggattttga agattttgga aacggcgctg cggatttttc tgcgttcaat
    23581 cttgtgcagg gtttgcctgt tttctatttg tattttcatt ggactctttc ttcccgttgc
    23641 cttccttctg gggcgcttcc ggataatcga tacgatgatg gaagatgccg gtcaggattt
    23701 tggtaaacgt ctccgcaatg gtatttaaat tgctgagcgt caattcacat tcatccagtt
    23761 gaccgtccat gtaaattcct tcgattcttt cccgcacgag cgacttgatt ctggccgggg
    23821 tcggattggt cagggtgcgt gaagaggctt caatgacgtc ggccagcatg atcagaccgg
    23881 cttctttggt ttgcggtttg gggccggggt agcgaaaatc gttttccgat agggagcgaa
    23941 gggattcatt cttgtccttc ttggctttgt catagaaaaa gctgataatg ctggttccat
    24001 gatgctgacg gatgatgttg ataatttgac ggcccagttt ggctttggcg gccagttcgc
    24061 agccgtcttt gacgtgagac atgatgatca ggctgctcat tttcggcgat aatttgtcgt
    24121 gccggttatc cgagttcggc tggttttcaa tgaaatactg cggttttttc agcttgccga
    24181 tatcatggta gtacgcgctg accttggtaa gcagagcatt ggcgctgatt gcttcagccg
    24241 ccgattccgc gagagacgaa acgataatgc tgtgatgata tgtgcccggc gcttcaataa
    24301 tcatttgctg aaaaagcggc tggttgagat tggccaactc caacagtttg atatccgtaa
    24361 taaaaccgaa caggctttca aacagcggcg tgatccccgc cacaagaatg ccggtcacga
    24421 taccgccgat gaatcccatg atcaatctga tcggcagatc attgaaaaaa tttcccgtga
    24481 tgagattaag gcagacaatc gccgccatat tgataatgcc cagaaagaga ccgatcttca
    24541 ggaagacgga acgctgacgg atgcgcacga tctgatagga tgcggcaatc gatcccagaa
    24601 aagaaaacag cgggaaaacg attttttcat cgaagaggaa tccgatgaga aaagacgtca
    24661 gcgcgctgat aatcagcgcg acattccggt tgatcagaat agcgatcgtc atggcgccca
    24721 cggcaaaggg gatggcgtaa taacaggcat ccaccggtat aaaagagaac gaccggttga
    24781 tcgcgatgga cataaaaatt ccgattttga taaaaaggat ttgcagcagg gcgatgatgc
    24841 cgaaaaccag caaatccagg ttggaccggt cggacgtttt gacccagttt cttgtgcgcc
    24901 acagataaag cacgatggaa agaaacatgg ccgtgaagaa aaatcccagc acgatcgcga
    24961 ctcgggtaaa tcggttgtcc tgatcgtctt taaagtaggc gtttagtttg gccagttcca
    25021 gatagccgat tttttctccc tcccggacga tcatctcatt tttttgtacc tggaaaaacg
    25081 tggcggctgc atcaccgatg atcgccattt tctttttctc agtggcgccc ttgttaaacg
    25141 ccaggttcgg ttcaataatt tttttcgtga tggagaatgc ggcatctttc aagggtttct
    25201 catcattccg gaaaatcatg ttgatggttt taagcagagg gtcgtcgatt tcattcatgt
    25261 tcagcagggc agacgtgtcc ctgatgtctt cggaaatctg ggttctcaaa ttccggataa
    25321 ccacgccttt ctgcttttcc gatttgccga aagcatcgct ggcgataaac cggttgtcat
    25381 aaaaggaaat gatcagttgg gaaaatttat tctgcaactc ctccgaaaat ctgtattcgt
    25441 tcaaaatata gaattcttcc ggcgtcaaat aggcgcccag attgttctcc agacgttttt
    25501 tctctttttg cagatccatg attttgtcgg gctgctgctg tttttcaggg acattgttgt
    25561 atcgctccgc ggcaagagaa aaagccttga cgatcttttt ttggatatta tcggcaatct
    25621 gactgttgta attgtaaata ggattgatat tttccgcgtc ctggattttt ttctcctcgg
    25681 cggcctgcgg gtcctccacc agaaaagcga agtccgcctt aatgttcttt ggagcaatca
    25741 tgccgatacg gaattgcggt ttggagaaat tcagttccgg cgccatgatg agggccagaa
    25801 tcagacacag caccacggcg atcatccagc gctggaaaag gctgtttctc acgaaaccga
    25861 aataaggtgc tgctttttcc agatagttcc tgaaaaattc cgtcattttt attctgagcg
    25921 attcaaaata tttcatgatc acacgttatg atgattggta ttttttgatt tagtcagatg
    25981 ggcataggcc ttgataatgt cctgaaccag cgggtgccgg atgacatcga tttccgaaaa
    26041 atgcacaaaa cggatgccgg cgcttttttt caaaatgttt tccgcttcga tcagaccgga
    26101 tactttttcc gttggcaggt ccacctgcgt gatatcgccg gtaatgacgg ctttggaccc
    26161 gaagcccaat cgggtcaaaa acattttcat ctgttccgac gtggtgtttt gcgcttcatc
    26221 gagtataata aacgagtcat tcagggttcg gccgcgcatg aaagccagcg gcgccacttc
    26281 aatcagacct ttgtgaatca gagcggtggc cctttccatg tccagcatgt cgtagagcgc
    26341 atcatagaga gggcgaagat aagggttgac cttttccgcc aggtctccgg gtaaaaatcc
    26401 gagcctttca ccggcttcca cggccggacg cgcgagaata atgcgttgga cctttctttc
    26461 cataaggcag gacaccgcca tggccatggc cagataggtt tttccggtgc ctgccgggcc
    26521 gatggagaag accatgtcgg catttctcat atattcaatg taaagccgct gggcgatgct
    26581 tttgggagtg atcacgcgtt tctgcgagga aataaaaacg gtgttcagga aaatactggc
    26641 cagctcgaag gtaacgtcct cggtcagaat acgatgcgca taatcgatat cggcggattg
    26701 aatgtgccag cctttttcca tgagggcata catctgctgc agcatcgttt tgattttatg
    26761 gacgttgccg tttcggccgg agatggtgac actgttgccc catgctttga ctttgaccgg
    26821 ttccagcttt tcaatgaatt ttaaattttt atcatgttcg ccgcagagat ttttcagcag
    26881 atgctgatcg gcaaagacaa ttttctccgc gtcttcattt tttaatgctt ttatcaaaat
    26941 agttatcaac ctctacaata ggtgatcaat atttcattca acgttggttt ctatcatttt
    27001 gatttcggca ttgcaatacc tattgataaa tccgtaaatg cttgaattat aagtgcttta
    27061 tgcataatgg tatgatgatg tggtctgttt tcataaggat gccctcatga tttccgcaaa
    27121 aacagatagg cgaagaccct ggcattcccg atcatatttt cgacggggca gttttcgtcg
    27181 ggttgatgag caaactggtt ggttttggac cagacggcaa ccggatagtt tttcttgcgg
    27241 acataggaag cgacggttcc ggctcccaca ccgccggtaa aggctttcac atgatagacg
    27301 gcgtcaatag cgtcttgcag cgccaggacc accggagcat ccgacggcgt cggttttgcg
    27361 gactggacgt actgaaccat ggacgtttcg atccgcacat tgaatttttt ctcaattttc
    27421 cggcagatct tttgtatggc attcattaca tccttaagat cgtattccgg caatacccgg
    27481 caatccatgc agaacacatc ttccccggga atcgtgttga tattaccgac attggcttct
    27541 ttctttgtcg gttcaaacgt gctttgcggc ggatcaaaaa gaggatcggt gtcgggaaac
    27601 atttttcgca gtttgatcag gctggtgacc agatgcgatc cggcaacaaa ggcgttgttg
    27661 cccaggtggg gggtcgatcc gtggcattgt ctgcctttgg ttttgaaaca gatccagagc
    27721 atgctttttt ccgcaacttc gatgagcgat ccttcggcgt tgccggagtc gggcacgaca
    27781 atccaatcgt tatcctgaaa aagtctgtcc gtagaatcaa tcatataaaa caatccctta
    27841 ctgctggagg tttcttcgtc ggacacgaac gccagtttga tcgagttttc gggaaccacg
    27901 ccttcatcca tcagcgcttt ggcggcaaag atggaggcga ccagatcctg ctgattgtct
    27961 tccacgccgc ggccgtaaat ccggccgtct ttcacgtagg ctttgtacgg atcgctgctc
    28021 cagaggcgga gttcgcccgg cgggacaata tccatatggg taatgatcca gacaaacctg
    28081 tttttgtttt tcccctcgat agtcgtgaca aaattggggc ggattccgga agacactctg
    28141 ttgtccggag cgtcgtagtg ctggaagttt ttgaatccca tctgcagcag acgatctttc
    28201 agaaaattgg ctttcacaag ttctccgtcg ccgccgtttt ccggtccgac ggcgggatgc
    28261 gcgcaaagcg ccgtctgcaa ttcaatcatc ccgtcacgat aagcatcgat ccgttgcgat
    28321 attttttgaa ataatttttc gtcgatcata attcatcctt tgatggtgat ttagtctata
    28381 ggggcgataa cggtgtagcc gcccggcgga agggggtggt ccggattttt gccgtctccc
    28441 agaatcagca ccgtattctt tgtctgatcc aggtatttaa cggcggcatg atggaggtcc
    28501 tgcacggtga cccggttgat cttctcccgg tagctcgtca gaaaatccgg aggcagtttt
    28561 tcatactcga tggtcatttg ctgccggacg atttgttccg gggtggcaaa tgaaaagagg
    28621 aacccgtcat tgattgattt ttgcgcccag gtcagttcct gaccggcaac cggaacggct
    28681 ttaatgtttt ccagaacggt attgatcagg gaaagagcct tcatggtcga cgatgttttt
    28741 gtaaaggcgt agctgccgaa aattccgtaa tcaggcctgg cgcggtaaaa acttccggca
    28801 ctgtaggcca gcccttcatt attgcgcacg gtgctcatga tccgggaagg aaatccgccg
    28861 ctgccgacga tgaaatccag gaccgtgaag gcatgaaaat cggggtggtt ttttccggtg
    28921 gcgaattggc cgctgataat cgtcgactgg ggaatatcct tttcgataaa atacataccg
    28981 gtttgcggtt gttgcaccgg cgcgggaaaa tccgtcgcac tgtttttcgg attccagccg
    29041 ccgaaatact gtttgatttt ctcaacggct ttttcctttg tgatgtcgcc gctgacggca
    29101 aacatgatgt tgttcggcag gaagaaacgg ctgtgaaacg ccgtcagatc atcgcgtttg
    29161 atttccgtaa gcgatgcgct ggacggaaaa cgtccgcggg ggtcttgatg atatatcaga
    29221 cgggagaatt cgcgcatggc cagcttttgc gggtcatcct tgatgcgcct gatttcctca
    29281 agttgtaatt gccgggccag ttccagtttc ccctgttcga aaacgggatg aatcagaatt
    29341 tgcgacaaca gatccagccc cttgtcaaga tgcgtggtga gaacggaaaa accgacatgc
    29401 gccgattcca tggccatggc aatggaagcc gatgccgcaa tgagatcaaa ttgtctgtcg
    29461 atttcccggc tgcttaatcg ggttgtaccg ccggttctca tgacctgagc ggtcagctcg
    29521 gccgtgcctt ctttaccggc aggatcatgc atcgagccgg ttttcacaaa ggcattgatg
    29581 gtcaccagag gcaggtcgtg atcttcgaga atgtaaagga tgatgccgtt ttccaaaacg
    29641 acccggtgcg ccttcggcaa ttcaaaaaac aagggttcgt attgaagctg gtcgggagaa
    29701 ggccttgccg cgtcggcctg gttagcgcta ctgggcctga cggtcgccat cattaaaaac
    29761 agaaacacgc caaccagaaa aaccggatat atttttgaaa tcattttatc catcagacgc
    29821 tacccgcctt ctttattgag aaaagctgtt gtgcggttat tgctgttcaa ataccttgcc
    29881 gcgatttgtc gaatgtctgc ggcagtgact ttgtcaatat tgtcgagata atctgcaaaa
    29941 tatctgtaat cccccagcag aacctcataa taggaaagaa tggaagcaat atcggcattg
    30001 gcatcgaggc tctgaaggta ggacattttg atgtgatttt ttgccttcat cagttcctcg
    30061 tcggaaacgg gttgagtctt cagcttttca atttccttca gaatatcatt ttccagctcg
    30121 atgttgctgt gcggatgacg cggttgagcg aacagggcaa atagattcgg gaatcttgaa
    30181 ccgggcaaac cgttgacggc actcacgctc tgggcaatct gccgatcgat gacaagcgtc
    30241 gtgtacaggc gactggttcg tcctctggtc aaaattgttt ctatgacatc gaagacgtaa
    30301 tcctcccgcg ccggagcatt cggtttgcga aagccgatga tcagcgacgg atttgcgtcg
    30361 aaggcaacat tgacccggcg ttcttcggtc tgaggcggtt ctgcgggaat ataactttcc
    30421 gttttttcgg ccgcggacgg aatgacgccg aaatatttct cgattaattt cagtgtcggt
    30481 tccggtttga tatcgccgac aaccgcaatc acaatgtttc ggggatcctt atatttttcc
    30541 agaatttttt ccagttcatc ggggcttaaa ttcatcaagt cttctgtcca gcccagaacc
    30601 ggcctcccgt aaggatgaag ggtgtacgcg gcgctcatga atttttcata aagttttccc
    30661 tccggatcgg attccacccg ctgccgtcgt tcttccatga caacgtcgcg ttccgtataa
    30721 aattcacgga agaccgaatg gaggaggcgg tccgattcaa ttcttgccca gagttcgatt
    30781 ttattggcgg gaagactgac gtgataggag gtgacatcct gaccggtgga cgcattcata
    30841 tcgagcccgc cgttttcggt atagagccgg tcgatttcgt tgggtaaaaa gtatttttta
    30901 tgttcgtcct gcaacttttt taatacagcg gaaaggttgg ccagggtttc cggatcggcc
    30961 gtttgtcctt tccgtttttc ggcatccaga cgccgtccgg catttttgat ttcttccatc
    31021 agggccttct cggcctgata atcgttcgtg ccgatggagg tggttccctt gaacattata
    31081 tgttccagga aatgcgccgc accgctctcg ccttttgttt catccaccgc gcccacccgg
    31141 tagcgaatgt agagggatac ggtgggactt aaatgccgct taagcatgag cacggtaatg
    31201 ccgttgggca gcttttgttt cagaaccttt tctttcagat cggaggcgtg accggagcct
    31261 gcggcaataa aaataaaaat gaaagccggc agaagagccg ctttcagtat tttacacgct
    31321 ttcatctttg ttcaaccctt cctgcttctg cggaaataaa cgaaagataa aataaaggat
    31381 tcccacaata caaaccagaa tcagaagcga tgcggaaaag aaatacatca aaaattccat
    31441 cggattattg atccggtacg accccatcag cgtattgatg ccgatgagca gaaaaaacag
    31501 cgacaccagc agaatcagca catttttaat ccatatcaaa accttcatga aggggcgcct
    31561 gtccttgaac cgtttttgag tttctttttc aattccgtat aacgcgcaaa actgaaatca
    31621 tcatttccgc agggaagcaa tgaattgtta tgatacaggg gttcgtgaag aatgtcaaag
    31681 ggtgaacagg ccacggcctc ttgccacatg gcggttccgg gaatcgggga gtattcggcc
    31741 agcaggggct tcgcgccgca ttcctgtaca aattcaatac tgttgatgac ctcccggacg
    31801 gtctgaccgg gcgttccgca cagcaggtaa atccccaggt cctctgtttt ataaccggcg
    31861 tttttcagat gtttaaccgc ctgacgcagt tcttcatttt taacttttcc gccggtttgc
    31921 cgctgccggt ccaaatcgga tgtttcaaaa ccgaaacgga tggtcttgaa tccggcatgg
    31981 tacagccgtt cggcaagttc ttttgtgatc tcccttaagt gcaatccgtt gggacaatgg
    32041 aaccggcagg ataaatttct tctttgtaat tcatcgagca ggggcacgat cattgcctcc
    32101 gggttaacca gcagagcgtc atcgtaaaaa ctgaaattca ccacgccggt tttcctgtgc
    32161 cagtattcga tttcatcggc gaccttcacc ggatcgcggc gcctaaatcc cggcgtcaga
    32221 atgtgagacg aacagtaact gcagcggtaa gggcatcccc gggacgtgat gaccggcagt
    32281 tgcccggttt tggagatcag atcaaaggcg gggtaggggc aggaatccag attgttttca
    32341 tccgggacaa acaacgtttt ttcgctaaat accttttcca gcaaatcgag aatttgtttt
    32401 tcgcccgccc ccgcgatgac gtaatccgcc cccgaaaagt tggacgcgtg atcgtaacag
    32461 agcgtcgcgt atttgccgcc caggacaacc ggaatctgcg gcaaggtgtc tttgatgatt
    32521 ctgatcgctt caaaaacgcc ggggtaccag taggtcatca tggacgtcat tagaatcatg
    32581 tcggcatccc ggtgcatttt cagctcggcg gcgaaaacgt ccggcagaat accgtaacgg
    32641 cagtaactgc gatcaaccca tttcagcggc gccggtttgt caatgatctg ccggtaaaat
    32701 ttaccgtctc cggaagcata acgccgcggt ttcttcagcc ccgatgccgc catctgcgga
    32761 tgaaacggat cgaggcagtc gatcaaatca atccggtggt tgtttttacg caaaagactg
    32821 cccaggtata gcaggccgag aggtttgatc cagaagtcat aggccgcaaa atcgtgaatc
    32881 cagggattaa tcaaaaggat ttttctgctc atcggctggt tcttatcaca atgcagattc
    32941 atttacaaaa gcctaaacgt ttatgattcg gcccggcgca gactgccgca ggaggggcaa
    33001 aatgaagggg cgtgatcaat tgacttcatt catcttttat gctaatcagt tccaccacgc
    33061 ataaaagtta ttgaaaatat gtagttattt attttgaggc ctgaaaagcg tgaaaaaaac
    33121 gacattgaca agtatttttt ttaggtcttt ttttattcat gcgacgctta atttccggcg
    33181 gatgcagaac atggggttcg gcatggcgct catcccgatg atccgggagt tgcgcctgca
    33241 gaaaaaggat gcggtaaaaa tcttaaccgc tcatttgcag atgttcaata cgcatccgta
    33301 cctgtcggcg ccgatcatcg gttcgatcgt gcgtcttgag gaagaccatg ttgcgaaagg
    33361 agaaaattcg gacaccgcgt ccatcagaca gagcctgatg gcctcttatg cggcaatcgg
    33421 cgatatcttt ttttggggag ccttcagacc gtttgccgcg atcgttgcgg tgctgctcgt
    33481 gcatatcggg ttggccatgg cgccggttat ctttcttctg atttacacgc cggcgcattt
    33541 atgggtcaga ttaaaaggat ttatcgaagg gtaccgcaag gggaaaaccg gttttgaatt
    33601 tatccgcgcc ctgaatttac ccgctgccgc cgtcagaata cgctggataa cgctgatggt
    33661 tctgaccggt ttaatgatct ggcttgcccg agacggcggg tactggccct tcggggaaac
    33721 atacggggtg gtcgcaaaac tgattgccct ggctacggta atgctttgtt atattttaat
    33781 aaaaaaagga atttcacagg tttatatcgt ttatggcgct gtgttggcat tcgtgattat
    33841 ttcctggacg ggatggatta gttgacggaa acgaaaacct tacgattaaa aaacaagctg
    33901 gggatgcacg cgcgggcggc ggcgtctttc gtgaaagtgg ctcagcagtt tcaatcggaa
    33961 atttatattg agcacaacgg gcaaaccgtt gacgggacca gcatcctgga tatcctgacc
    34021 gtggcctgcc cgtacggcgg agagctgacg atcaaagccg acggcgcgga cgcagccgcg
    34081 gcgattgagg aactggtcaa actgatcgaa aataaatttg gagaagaata aacatcatat
    34141 gagtccggat cagaccacaa aaacttttgt cctcaaggga gtgggtgttt cccccgggat
    34201 cgctattgga aaatgctacc gctatgatcc gctggacagt caaatttctt tttataaact
    34261 caaagacgaa tctttaatcc cggaagagat aagccgtttt aaaaacgcct tgaaggaatc
    34321 cgagcagcaa ctgctgaaga ttcagaaaaa tcttaagaaa gccaatgttg cggaaccggt
    34381 ttatgtcatc gacgttcaca tcctgatttt gaaagacaaa atctttatca accgcaccat
    34441 caaatatatt cgcgagatga gcgtcaatgc cgaatgggcg ctgcgcatga ccctggatca
    34501 ttataaagag gtgttcgaag gcgttgaaga cgattacatc agcagccggg tcagcgatgt
    34561 gcagtatgtt gtccagcgga ttttgcgcaa tctgtccggc ggaaaaagtg aaatcgtctg
    34621 ggaaacggaa aaccggggcg tcattatcgt atcgcacgac atctcaccgg ccgatacggc
    34681 gcagatgaaa ctggacaaaa ttctgggatt tgccacggac agcggcgggc gcacctcgca
    34741 tacggccatt gtcgcgcgct ctctggagct gccggccgtt gtggggctgg atgatatcac
    34801 gcgctttgtt catgaggatg atgacttgat cgtcgacggc acggccggca tggtggtgat
    34861 caatccctat cccgacatgc tcaagcggta tgaggaaaag aaacgccatt acgacgccgc
    34921 aaacgatgaa tatctcaaat acggcaggca gcccgcggtc agcctggatc agcaccgtat
    34981 tcaaatcgga tgtaacatag aatttataga agaaattcct tccgccgtcg ctcacggcgc
    35041 tgaaaatatc ggcctgtacc gcacggaatt tctctacatt taccgcgaaa acttgccgac
    35101 cgaagaggat catttcacga attaccgcca ggtcgtgacc aataaaaatc tggcctggtc
    35161 gacgatccgg acgtttgatc tgggcggcga caaatttccc aattatcaga aacagagtaa
    35221 ggaaatgaat ccccagatgg gcatgagggc gatccgcttc tgcctgaagg aagtcgacat
    35281 tttcaagatt cagctccggg ccatctggcg cgccagtgcg ctgggcaaca ttaaaattct
    35341 cttcccgatg atttccagtg tggacgaaat ccgggaagcc aaaaaaattc tggaagaagc
    35401 ccgccgggaa ctgatcgatg aaggaatgaa gattcctgaa aaaattgaca tcggtgcgat
    35461 gattgaagtg ccgtcagcgg ttgtgattgc cgataaaatc gcgcgggagg ttgatttctt
    35521 cagtatcggg accaatgatc tgatccagta ttcgctggcg attgaccgcg ccaacgaacg
    35581 cctcacctat ctgtacgagc ccctgcatcc ggctgtcttg agacagatta aaaaagtcgt
    35641 ggatgcggcg cacaaggcga agatcgaggt gtccatgtgc ggcgaaatgg cgggtgatcc
    35701 gctttgtgtg ctggtccttc tcggtatgga actcgatgaa ttgagcatga atcatctggc
    35761 cattccgaga atcaagaaaa ttatcagaga atcgacattg aaagagtcga aaagcatgtt
    35821 gaaacgagcg ctttccttca atacggcttc ggaagtccgc tcttatgtgc agaaatatat
    35881 ggcgaggcgt ttccccgaag atttcaagac ggaagattaa tgtacgggac cgcccgtcaa
    35941 agttgtgtgg agaaataacg acgatggaag aaaataatca tccggtaaaa tccaatcagg
    36001 gcattcaata ctattcgggt ctgctttacg acaatcagaa ttttctgcaa ttccggctgg
    36061 ccaagacgtt tctgtcgcgt gtttccattg ccgacgaata cgttgaaaat atcaaaaaac
    36121 tttccggcga gggcgtggtc gtttatgcct taaaccagcg cagcgaactc aacagcctta
    36181 ttctgtatga gctgctccag cgcaacgaca tgcccgttcc ggtctattgc cacggcatga
    36241 acatgtcgtt ggctcatccg ttgccgcaaa tgctgaaatt tttctggtcg tctttaaaac
    36301 gccggatggg cagggaacga aaagcctggc agggcaaact ggccttcatt gaaaaaacaa
    36361 tcatggagaa aaagagcatc gtgattcatc tgggcgaatc ggagttcatt gaagaccggt
    36421 cggcggaaag cgcgatgtcc accttgcttg atattcagaa aaaagccggt tttccggttt
    36481 ttgtcgtgcc gatgctggta tcgtacgggc gccgccgtga aaaagaagac gaggggctga
    36541 tcaatatcct tttcgggcag accgagcaca ccggggcgct gcgccgtctg atcacgttta
    36601 tccgatattc ccgaagcgca tttgtcattc cctcggagcc ggtgaatttg tcggagtatc
    36661 tgaaaacgac caagaaaacc gccacggacg ccatggttca tgaactgcgc ggagaactga
    36721 ttgatcgcat cgacaaggaa aagacggcca ttgtcggtcc cgccctgaaa tcgcgggaag
    36781 aactgatcgg catggtttta agcgatgaat cgctgaaagc ctttatcgaa aagcatgcgc
    36841 aggaggagaa aaaagacaag gccaggatta aaaaagatgc acgccgttat ctgtatgaaa
    36901 tagcggccga ctacagcgaa accttcatcg ccatctggaa aaaagtgctt acttggctgt
    36961 ggaacgatat ttacgacggc ctgtccattg atgtggaagg catggccaaa ataagaaata
    37021 tttccaaaaa aatgccgttt gtgattgtgc cctgccatcg cagtcacatt gattatcttc
    37081 tgattcatta cgtcttctat atcaataaca ttcagcttcc ttttatcgca gccggaaaca
    37141 acctgtcgtt tttcccgatg gggtacattt tccggaaatc cggcgcattc tttttgagac
    37201 gaagctttcg cggcaacaag gtttattccg aagcctttac caaatatctg gcgatccttc
    37261 tcaaggaagg actgccgctg gaatttttca ttgaaggcgg gcgcagccgg accggcaaaa
    37321 tggtgatgcc caaatacggc cttctttcca tggtgcttca ggcctatcag gagaaatact
    37381 gcgaaaactt tgccgcgatt ccggtgtata tcggttacga ccgggtgatc gaagaaaaat
    37441 catacctgaa ggaactgtcc ggcgaaacca agacgccgga aaacacggcc gagatcatta
    37501 aaaccagcaa aatactgacc aaacgattcg gccgcgtgta tgtgaacatc ggcgaaccga
    37561 tgatcatgaa agactatctg gcgcagcagg caaaaccgct ggaagacatg tccctggagg
    37621 agaggcaaag tctctaccgg aaaatcggtt atgaaatcgt tctggaaatc aacaatgtgt
    37681 ccgtggtcac gccttttgct ctggtggccg ccgttatttt aagtcatgac cgccgcggca
    37741 tttcctacaa cgatctgctg tatgttttga atgaatttta cgaatatctc accgtcaaac
    37801 aggtgaaatt tgcggaaacc ttcgcccatc gcgacaaggc catcatcgat gcgctgaaca
    37861 tgtttgtgca ggcaggcttc attgaaaaaa ttgaagcgga aaaagatgaa gtcgaggaaa
    37921 tgcaggaagt cgtctactcg ctgaaggagg aacgaagact caatctggaa tattataaaa
    37981 acaatattct gcatttcttt gtcccgctgt gttttgtcgc cacctccatc atcaaaaaca
    38041 atgaagacct gatttcgctg aatcgcatac agggcgacta cgggtttctg aaaaaattat
    38101 tatggaatga atttattttt gacaaacaca aggacgaagc ggaggaagtc aatgaagtgc
    38161 tgtcttattt atacgaccgg aaaatcattg tctcggtcga acaggacgga cagaccctga
    38221 tcgaaatcaa aggccggggc aacaaacgac ttaaaccgct cgccgatctg attcacaatt
    38281 atctggaatc cttctggatc gtgatgagaa gctgtctgta tctgaaaaaa aatcctctgc
    38341 ccaaaaaaga atggctgaag aaaatcatgg cgctgggcga ccggatgtac cgcaagggag
    38401 aggtgctgag aaaagaagcg ctatcgcagg ccaattatca aaacgttatc atctatctgg
    38461 aagacgcgga tctcatcaaa tcgttccggg atgagaaaat cgataaaaaa gatgttttat
    38521 acacgctggc tgaaaacaga gcggaaatgg aagtgctgcg ccgccgcctt ttcagatttt
    38581 tataatcatt gaccgccgcc gatgcgaaag ccgcatcgcc gatggggttg atcggatcgg
    38641 gttcagaccg ggagagccgg ccttattcaa aaggccggct cactgcatgt tgcggggtta
    38701 tctttttctg ccggatttcg ccggttgttt catgttatag aaggtctctt tgccgcgata
    38761 cacagccgca tcaccgagtt cttcttcgat gcggatcaac tggttgtatt tggccagccg
    38821 gtccgtgcgg gagagcgagc cgcttttgat ctggccgcag ttggtggcca cggcgatatc
    38881 cgccataaag gtgtcttccg tctcgccgga gcggtgcgaa accacgcacg tgtagccgca
    38941 ttccttggcg cggctcatgg tttccagcgt ttcggtcagc gtaccgatct gattgagttt
    39001 gatgagaata gagttggcga ttcccagatc aatgcctttg ttgagacgtt tgacgttggt
    39061 aacgaagagg tcgtctccga cgatctgaat tgtgccgccc agttcttccg tcatcacttt
    39121 ccacccggac cagtcgtctt ccgccaggcc gtcctcaatg gaaatgatcg ggtatttggc
    39181 aaccaactcg gcataaaatt tgaccagctc ttcagacgac ttccggggct tggcttccgc
    39241 cgccagaatg tatttccctt tttcatagaa agaactggct gcgcagtcca gggcgatcat
    39301 gatatcccgg cctgctttat aattggcctt gtcgatagcg gtcataatgc atgcgagagc
    39361 ttcttcattg gatttcagat tcggagcaaa gccgccttcg tcgccgacag aggtattata
    39421 gcctttacct ttcaggacag acttcaacgc atgaaagact tccgcgttca tgcggatgcc
    39481 ttccttgaag ttgggcgcgc cgaccggcag gatcatgaat tcctggatgt ccacgttgtt
    39541 gtcggcatgt tgtccgccgt tgagaatgtt ggactgcgga acgggcagaa catgggagtt
    39601 gacgccgccc agatagcgat acaacggaag cccggatgct tccgcgccgg ccttggcgca
    39661 ggccatggag acggcgagaa tggcgttggc gccgagtttc cccttgttgg cggtgccgtc
    39721 catttcgatc atggtaaaat cgatgtcgcg ctgtctgcgg caatccattc caatgatttc
    39781 cggaccgatt ttgtgcagga tgttatcgac ggctttttca acgcctttgc cgttgtatcg
    39841 ttttttgttg ccgtcgcgca attccagcat ttcatgttct ccggtggacg cgccggacgg
    39901 caccgcggcc cgcgcggtga ttcccgatag cgtcgttacc tcggcttcga cggtcggatt
    39961 gcctctggaa tccagtatct ccctggcatg aatatggatg atctccggca taaaacacct
    40021 ccctgatgaa ctttcctgga atggtaacag agaggccttg cgatttcaac agattgatga
    40081 aacaaacttg attacttttc ataactcata tagatagaac ccggtccgtg agaaaaatgg
    40141 agtcaatgga atttaagatg aatcaggata caaaagacag gaacaccaaa cttttcctgc
    40201 atatgaaaaa atggctggaa aaagccgtgc tggattacaa aatgatccaa aagggcgatc
    40261 gtctgttaat cggcgtgtcc ggcggggcgg acagtctggc gctgctggac ctgctcgact
    40321 ctccgatgct tttcattccc gatttttcct tcgtggccgt caacattgac atgggattcg
    40381 accccgcata taaaaaatac gacagactgg aaaaatattt tcagaaaaat aattatccgt
    40441 atgtgatgga aaagaccgat atcggcactg tggcgcattc cgatgtgaat aaaaaaaatc
    40501 cctgcttttt gtgttcccgg ctaagacgaa aaagaatatt cgagatagcc gatgccgagg
    40561 gctgcaataa aatcgccttt gcccatcacc gcgacgatat tatcgaaacc ctgctgatca
    40621 acatgtttta cgggcgtgaa atcagcacca tgatgcccaa tcagagcatc ttcggcggta
    40681 agctgcacat catccgcccg ctggtttatc tgagcgaggc cctggttaaa aaatacagca
    40741 gggaacggcg atttcctgtt ttgaaaaatg actgcccgac aagcaaaaca tcaaagaggg
    40801 tgtatatcaa aaaccttctc aataaactgg aacgggacaa caaggaaatc cgcgataaca
    40861 tttacaaggc catgagccac gtcaaaccgg actatttgct gccgcaaaaa aagtgaaaag
    40921 agaatggtga atagtgaatg ttgtatggta gacagtctga agaaaaaact atagcaatac
    40981 atcgtatttc atgtaaaatt gcattatggc aaaagagaaa acagcacaaa aactgatcgc
    41041 gtccaataaa atggcgcggg tgaattatga aatcggcgat atctacgaag cgggtcttgt
    41101 tttaaccggc acggaagtca aatcgttacg agccggcaaa gccaatctca aggacagcta
    41161 tgccgttgtc aaagacgatg aagtctacat caaggaaatg cacatcagcc cctacgattt
    41221 cggcaaccgc tccaatcaca atccgctccg gacccgcaaa ctgcttttgc acaagcgcga
    41281 aatcaaaaaa ctctacggga aatcgcgcga gaagggattg tcgcttattc cgctcaagct
    41341 ttatttcaaa aacggcaagg tcaaagtgga aatcggcatc ggcaagggca agaaacttta
    41401 tgaccgccgt caggatatca agcagcgcga agaaagacgc tcactatccc gcgatttcaa
    41461 atccaggcag ataaaagtct agtgattatg aatccatatg acacggatta aggtgaaatt
    41521 tgacaaaaca cttctaagtg tttatattac atgaaaatac gtaatagggg ggcgtcacgg
    41581 tttcgacggg gacatttgaa gttgtagaag cgtgccgagg ttcctacagg cctcgttaaa
    41641 caggtaggaa acatataatc gcagacaact acgattacgc tttagccgct taagtcggct
    41701 aacgttccac ccggttttcc tgttggctgg actggaacgt cattcagaca ggatagccaa
    41761 acttcttgcc tgggggaggg cggcgaactc ttacaggata gcgcggggag atccggcctt
    41821 agggagcaaa ccagcgtgaa ggttctaaaa cgaaggctac gcacgtagaa gctccagcgg
    41881 aaggttttcg gacgcgggtt cgactcccgc cgcctccacc aaaaggtatt acaacatcta
    41941 atagtcattt tgtaaaactg tatagtttat tgactatatt caccatgccc atggtatcct
    42001 gaccgcaata ttcttccaga tactttctga ccttatcctt atctttattg tcttcggtaa
    42061 atgtaatccg gtaatactct gagctggcca tgccgccatc gccgatttcc atatcgtcat
    42121 aggatttgcc tgtcatcgcc ggcaggacat tcttgataga acagctgccg tcctgtgccg
    42181 ggtgataata ggcaaaggct ctgaaaggct ggagcaggtc aatcatcctt tcgtttatag
    42241 attccaccca atttttatat tgtggaagga cttctgcaca ttttctaagg atgcccgttt
    42301 caaaacccgc attgtaggcg attacggaac cttttgtgcc catcacgtct ttgagcctgg
    42361 ccataaattc agggcgcgga tcattcgtgc catctgccag aaaagagaag tgttcaggtt
    42421 tagcgccttt cttttgtaca acgtggacag agaactgaaa cggtatttgc tgatatggac
    42481 tggaattgtc aaatggcgga ataacagatc cgcatgtttc aaaatccatg taataggcgg
    42541 gatattgaat tgtatctaga aattccttta gctttttcga atcaatataa ggattgcccg
    42601 acttttcaca atcaacctgt atttgatgtt ttttattcag ttcataatct tctggtattt
    42661 tcgctaattc aagaatgcca tcccgaatca aagtcatggg aagtttgttg ttgctgtaaa
    42721 gaagacagac atttctttcg ggaaggaatg accagcattg agctgttaac ggacattcat
    42781 aagggtcact gcagtgaggg ccaatgccga tttcaatgta tgtttcagag ccgatggctt
    42841 taatcatctc ctccacattg ccttcaatca ttttgctgta cgtttttttt atttcttcag
    42901 tgatatcgat ttttttaaat atttttttcg gcgctatgtc acccttacgg acatacttgt
    42961 tatcaatgca cattaagtaa catttgttga tctttaaccc ggcaccggaa taaacatagt
    43021 actgaaagcc gatgtcggga taattaatgt cctttatatc tgtcgagctt ttgacttcaa
    43081 tcaaatccca tttatctttg ccgacgggtt ccaggatatc cgcccgtgca tagcaattct
    43141 tataggccag ggccgcttca aatatcggaa ctctttgcga taagaattcc tgcgtcgatt
    43201 taagacctct ttcaaatgat cctgtatgat caacctccgc accgttagga aacaatcgct
    43261 tggcaaaatc gccaaccaaa tgaccttgat caaagatagc ctgtttggct tcatccggtg
    43321 ccggaatcgc atccggttca ttgtaaatat accagagcag ttttttacat tgcagaccgt
    43381 tgagatattt tgttttggat attcgaatgt tcattttgat gttgttatgc gtaattaatt
    43441 aaaaagaaag taaaatcgct taaccaattt ggctatttaa tacaggcaaa tttttctttt
    43501 ctttaatata gtggatcgtc tgtaagtaaa cttcaagaag agatgataac tctttaggtt
    43561 caacaactat tgctgatact gtaaaattcc cttttagtat aattgctctt gcctgtttat
    43621 cataagcatg gtttgtaacc cacggactct tctcaaattg attaacatca ttaggcgttt
    43681 tgagatgatc ccatattctg aaagcaagat ttccatttgc agccttacct atataaataa
    43741 tgttatcttt attatcgcag aaaatatata cgccgctgct gaatttatgt gtggccgatg
    43801 gaggatcact ttctcggaag ctatctttga atagtttctt atactttaca aattcgatat
    43861 caaaattaag ttctggtatt tgtttgttta cgatttcttt aaagtattca gatgagtcaa
    43921 ataattcttg aagtttcatt tcgtatctcc ttttttaatt tgaacaatat ttgtatataa
    43981 aatctttcaa ttatttatac tttctatacc cattatagca tcgttcacct tttttctaaa
    44041 ataatcagtt cttgcttcca tgaatgcctt gatgaatctg aaatcaaacc tgcaggaatc
    44101 gccgagtctg tctttcagat tatgagataa taattcgatc acggcataat ttaccgagca
    44161 ttcccgattc tcccaatcgg catccaatgc cgcactttcc cacaatattt tcatatgcca
    44221 tagttttgat ataagatgat aatcatcagt ccgtaccttt gtgcaaaggg gtatcttgct
    44281 tttattgagt aaaagcatca tcatccgtat aacttttctg tagtcataga agatatattc
    44341 actgtattcc cacgcgtttt ttataaagta aaaatagtgt tcgccccaga tatccaattc
    44401 atcaatatcg ggagaaaata tctttttaag atattcttct tcctcaagcg aaaatatctc
    44461 aagggatgtc agttgcttga tagtaaacct gccattatac aagtctttat ccttgataat
    44521 gtttaccact ctacagatca acgcatggta tatgtttttg tcttcaataa tgattgaata
    44581 ccgccccatg attaaggcct ttttatctga tgtttatttt gtgaatatta ttccctaaag
    44641 tcgttccata atacggcacg attaattgat tattttattc agttcattat ttagcgaaga
    44701 gcgtttttga aaagatcaaa tgtatcgaga gtgctttatt ggtttaaaaa tgtatcaata
    44761 aattcccccg atttttttgc atcattaaaa tacttctttg acaagctacg ataaaataaa
    44821 ttaccatcgc atggtttcgt accatactac aaaaagaact ccataccgct acttttcaac
    44881 aagaaggcgg cgattgcata caagataaac aaagaaaata aacaaagaaa atactatcct
    44941 gaatctaaat ttatggtatt ataataacca ataagggagg gatgttatga atacctatga
    45001 cctgatagag accaaaccgg aagtgatgct ggggaaaccc gtcatcaaag ggaccagaat
    45061 tccggttgac ctcattgtaa gaaaattggg tgaaggcgcc tcttttgaag atttactcga
    45121 tggttatcca aatctgtcga gagaagccat tcaggcggcg ctgatttacg ccgccgatat
    45181 gatccgcaat gaaacaacta tctttttaaa gacaggcact tccaattgaa tacaacaaag
    45241 ccattgagct ttttggcaga cgaaagttgc gatttttccg tcattcgagc actgcggtca
    45301 gtaggctatt ccgtcaaggc catcgctgaa atatccccat cactaccaga tgaagcggta
    45361 ttagaattgg cggtggcgga aaaacggctg ctgataacag aagacaagga ctttggtgag
    45421 tgggtatttg cgcatcaaca tgtgatggcg ggcgtattgc taatccgcta cccgtcgagc
    45481 acgcgttcct caatgggtgc agccgtaatc gatctgattg gaggacatgc accagaactt
    45541 gacggtagct ttactgtttt agagcctgga agagcgcgaa tcaggacgaa aattaaagac
    45601 tgaccgccac aggtcgcaac atataaactg agaaagacct tataactgct gagcacctca
    45661 caggtgataa acttttacaa gagcctttct tttagatttc aactttctac cttggcgaat
    45721 gtattccatg ctttttctac tgagccttaa ttttttacac atagtttcaa gatcgcaatt
    45781 cttcatacaa gcccattcct gacaaaaaac aatcatgata tcgttccata atacggcatg
    45841 atttattatt tttagattta catcagaaat acgataagtt atattattaa ccaataggac
    45901 catataattt tgaacacacg gaggaaccat gcccgaaaaa tttgatccct ataaaagtta
    45961 cggtgaaaag cttatcggtt tgtttgtgcg cctgcttttt tcgggcgaaa gccattcgtt
    46021 gaccgacctg gcccggatgc tggactgctc caagcaaacg gttcttcgat tgattgataa
    46081 catccgcaaa gcctacgaca tcaatattga agagacaaag cagggcaaca gaagttttta
    46141 ccggatcaga aaagccaccg cgtctctcca gaacattccc cttaccgaag cggaactgca
    46201 atccctccag ctttgcaaag cctttacccg gcatctggtt ggcaagaagc tttacgagga
    46261 agcaacacag gccattttaa agagcaagac ctcgctggca gcaggtcatc aggtggggga
    46321 atgccatttt accgccttcc gtccggggac tatcgattat acgccgcagc atcagatcat
    46381 ccacaccttg attgacgcga tggaaaagca caaggtctgc aagctgacct accaatccat
    46441 gaattcgccc cgaccgaaaa catattacat caagcctttg aaactctttt cccatcacga
    46501 cgcgatttat ctgcacgcgc agaaggcaaa agagccgggc aaaccgtacc gcgcgcccca
    46561 atatgatcca ttgctcgcgg tgcaccgcat gaaagcgata gaattgacca atatcagttt
    46621 tcagccggat aaatttgact tcgagaaggc gttcaatcag cagttcggca ttatcaagga
    46681 tgaagcgttc agggtcgagg cacaattcac cggctgggcg gcttcttttg ttttagagcg
    46741 catctggagc ccggatcagg aaattgtgaa aaaaggcaaa aataaaatcc ttctgaaatt
    46801 taccgcctct tcggagccgg aactgatcag ctggctgctt tccttcggtg aacaggccac
    46861 tctcatcaaa ccggattggc tggtcaaaaa actgaagtta gctatagggg gaatggaagc
    46921 ctgttatagt tgaaataaca ggcctattgt tcctgaagtt tatcggatga aaatcaaagt
    46981 tcgtcatctg ctttttaaat aacccttcag atcacgatag tagttctcat gtggaccaat
    47041 catgatgagt tctaaatccg aacctgcttt tctgtatgct aaaaggtata aatttgtttt
    47101 gagcttgaat ttatgaacga aaacaccttt cagatcgccc tttttttctt cgccaacatt
    47161 agggttttcg gcaatgtttt ttacttctcg atctaaagcg gttttttcag ctttagacat
    47221 cttcttaatt tttttttcga ataattttga ttggtagatt ttcatgagaa tctatccaaa
    47281 ccggtattcc gttttatcgc cttgatccaa ctcatccagt cccaatagga tttctttaat
    47341 gagggaatat gacaaatccg gattttcctc tgcacatttt cccatacgcg cccaatgctc
    47401 aatctgaccg gctaaagaac gatggtctat ccggctaaat ctttttgcct catcaacaag
    47461 ttctgctgat attctaactg ctgtagccat agtttttcct ccatacaaat ttctaatagt
    47521 tgcaatatat atcaaatagc aacattatgc aacatcttgt tgtatatcgt tttgcgggta
    47581 acattctaga tgcaaaatta ttacagccat gaccagcgac ctttccatgt cttcgtaata
    47641 actaaaagat caccgcaccc tttcaatcaa taaaaaatat ttcaattgtt ccgtaatatg
    47701 gaacgatcct ccggtaagct attcccaaat cagacaacaa aaaggagaaa attatcatgg
    47761 ccaaagagga cattaagcgg gaggacaagg cattagcgca atacggcatc atctatttat
    47821 ccggcgatat tgacacgg
//
LOCUS       DAOSME010000012        44863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1083_length_44863_cov_24.106968, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000012 DAOSME010000000
VERSION     DAOSME010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 44863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44863
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_44863_cov_24.106968"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1243
                     /locus_tag="PLG98_03105"
     CDS             341..1243
                     /locus_tag="PLG98_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HOE32268.1"
                     /translation="MNIGELLGAMVQTGMSPSSSARMKNSLGGGKELDSLKDMLGDAA
                     GQSGSGGLGGLLSSVLGGDKSSGGKSGGIGDLLSGVLGGGQKGGASSSGGIGDLLSNM
                     LGDAGKAVGGNKNLAIGGLGALIGSIMGGGGKSAGGAVGGGLMALLGALAYNALKGIG
                     QAKTPIPAGLIEPGTDEEQKQLDQSSELVLRAMINATKADGKIDQNEINRIMGKFNEI
                     GVEEEGKKYLMMQLQQPMETDTLIAAAQGQPDLAAQMYAASLMAIEVDTQNEKDYLEK
                     LAEGLGLGSQVTTRIEQMVGLQPS"
     gene            1502..2137
                     /locus_tag="PLG98_03110"
     CDS             1502..2137
                     /locus_tag="PLG98_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOE32269.1"
                     /translation="MIKTIFWDNDGVLVDTEGLFFRANQEILAGLGIPLQREQFEEIS
                     LVRGESVMRLAATPGTDAFIKLRRDRDALYADLLTRDPLVIDGVREVLDALQGKYLMG
                     IVTSSGHEHFEIIHRQSSLLPYFQFFLTLKDCPYTKPHPGPYLKAIALSGFAPEECLV
                     IEDSPRGLAAATSAGLRCIVIPSKFTCNHDFAGACKVLDSIKGLPAFLKEF"
     regulatory      2191..2283
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2365..3135
                     /locus_tag="PLG98_03115"
     CDS             2365..3135
                     /locus_tag="PLG98_03115"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOE32270.1"
                     /translation="MLNEIIISRAILDSYFKKLGSCLDLDVAIVGGGPSGLVAGYYLA
                     RAGRKVALFERKLSIGGGIWGGGMMFNSIVVQEAGRQLLEEFDLQGREYTPGYYVLDA
                     VDITSTLIHKAVRAGLQVFNLIAIEDVVIKNERVAGLVINWGAVDTLKWHVDPLTVHA
                     RYVIDGTGHPANVTEVLVRKMGVRLNTSTGGIVGEKSMEAGKGELQTVENTREVYPGL
                     FVSGMAANAVYGGYRMGPVFGGMLLSGLKVAEEILARL"
     gene            3156..3782
                     /gene="thiE"
                     /locus_tag="PLG98_03120"
     CDS             3156..3782
                     /gene="thiE"
                     /locus_tag="PLG98_03120"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOE32271.1"
                     /translation="MRTFLRHGLYLILTDPRDGYETLCRWAVEAGLPAVQLRYKGHNE
                     REHLALARALRQITKGTGTLFIVNDRPDIALISQADGVHLGQDDLPAAEVRQLIGPTM
                     LLGLSTHNLEQVAAANAVPVDYIGFGPLFATNSKERLAPVTGPDILKAALAISRYPIV
                     AIGGLTLERIRQLQPRPHNTAVIRAVNDASDPLAVMRAIHESCMNRTS"
     gene            complement(3789..5057)
                     /locus_tag="PLG98_03125"
     CDS             complement(3789..5057)
                     /locus_tag="PLG98_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOE32272.1"
                     /translation="MEKTLLIIFLILFVGRILWRFILQQLNIRHLRQCGKIIPPAFRN
                     VIDQATLSKMADYTYDNLRLENKENSTEDLLELAIIFLFLPLLVAKTNGLQLHLIWQA
                     LVFFGTLALIGGVIGLPFDIYRTFVLEKKYGFSTITWKLWLTDLVKSVIISAILMGFL
                     LSVFMAFVLYLPTSWWFWAWIFFAAFEILLLWLFPVVIAPLFNKYEPVKDEELKEKIT
                     SLLSKVGLKAKGIFQVDEGKRSKHTNAYFTGIGKTKRIVLFDTLLASHTHDEILAVLA
                     HEIGHWKKKHILKQLIFMVAASFVGFYLVYLLVNWPPIYGAFGLPQTPVYAGLLLVSL
                     YFSCVGFFFSPLAAFISRRYEREADRMAHELTGTSEPMIHALKRLAKDNLSNLHPHPW
                     YVWFYYSHPPLIERIEYLKTMDKGNLQRQR"
     gene            complement(5139..5855)
                     /locus_tag="PLG98_03130"
     CDS             complement(5139..5855)
                     /locus_tag="PLG98_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE32273.1"
                     /translation="MIPKIIHQTWKSTEIPPRWQRFRDAWKKFHPGWEFRLWTDEDNR
                     RLVERDYTAFLQLYDSYAYPVQKADFARILYLHKFGGVYADLDLEPLRPLDGIIDQPH
                     LILGEEKEGMGKSLNRCDFIINAFMASPKGHPFWEMLLERLRVTFRHKRLLQSKGHYI
                     IQTVIFELDKLARDYQQTHQDITIYPYEVFYPLSWHEACSELERSKAVEMSYTIHHFD
                     TTWFSGGMMLMKKLRRRLNI"
     gene            6325..7965
                     /locus_tag="PLG98_03135"
     CDS             6325..7965
                     /locus_tag="PLG98_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE32274.1"
                     /translation="MSNFVKKTIGQLVRETAECYPQNPALVCPDFGIRQTYSEFYQTC
                     RDVAKGLIALGIKRGDNVAVWTTNIPDWIHLQFALGMTGGILVTVNTNYQTHELEYIL
                     KQSDSTTLFLMESYREVNFYENVRKILPELDHHQPGTPVSAQLPALKNIIYIGKREST
                     PGMLHFSDIITMGREVTDKQLEDRLHSLHDDDVINMQYTSGTTGFPKGVMLTHHNIVN
                     NARMVGDVMGMTEKDKLLIQVPLFHCFGCVMSSLNCVYHGSTMVVVEYFDPLKAVQWI
                     SAEHCTAVNGVPTMFIAILNHPDFDKYDMSSLRTGIMAGAPCPVETMNQVRTKMHCPE
                     IVIAFGQTESAPVMTMTRRDDPVELRVSTVGRLLPDIEGKIVDPDLGIDLPPNAQGEI
                     VTRSECVMKGYYKMPDATADAIDRYNWLHTGDLGEMDDNGYFKVTGRIKDMIIRGGEN
                     IYPRELEEFLFTHPKVVNVQVIGIPDKKYGEQVLAAIQLKAGQTATPEEFTEFCQGKI
                     ARHKIPKYWEFVDDYPTTASGKIQKFKMKEVFEKKYPA"
     gene            7992..9548
                     /locus_tag="PLG98_03140"
     CDS             7992..9548
                     /locus_tag="PLG98_03140"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HOE32275.1"
                     /translation="MSNIINRDTIGDMARRAATKYGDKTAIIFRNIKLSFYDLEREAR
                     RFANLMTRYGVRKGDRVAVYAYNSHYYPISMMGLAKIGAIQVPINYMLNAEEIAFIIN
                     HSGSRLFIIEDTLYPVIAGCKDKFPAVEKWGFIPLNDSGIPDGFFNLIAEMSAMNYDE
                     PQVEVSAEDIVQIPYTSGTEAKPKGAMLSHRALMSQYHSCIFDGRYETDDVSLHALPL
                     FHCAQLHCFLMPYLYVGATNVIMHKADPVEMIRLIEKYQITHMFAPPTVWIAILNHPE
                     FNNYNHGSLKKAAYGASIMPVEIIKQLSVTFKGLKLWNYYGQTEMGPVATILKPEDQL
                     LKPGSAGKPVLNVETRLMDDDGKFVALGDVGEIVHRSGHVMSGYLNDPEKTAEAFQFG
                     WFHSGDLGRFDNDGYLYVVDRKKDMIKTGGENVASREVEEVLYQYPGIEEVAVIGLPD
                     PKWIEVVAVVAVLKKGVSIQEKDIISFCKDRLAGFKCPKKVFITDRLPKNPSGKILKR
                     ELKEQLSSSI"
     gene            9564..9953
                     /locus_tag="PLG98_03145"
     CDS             9564..9953
                     /locus_tag="PLG98_03145"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOE32276.1"
                     /translation="MQDTQETFTSIGEIAKKLDMSQRTIRYYEEIGLLNSIKRVEGGR
                     RIYTEADFRRLKLIKRLKIMGMTLSEMQELEAMWTIEKSNDKVLGRLLELLNNQLRRL
                     DDRIADLNILRNEIVEYQKRIKSKIGK"
     gene            10002..12089
                     /locus_tag="PLG98_03150"
     CDS             10002..12089
                     /locus_tag="PLG98_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOE32277.1"
                     /translation="MEKFFYPRSMVVIGASVKRLNLGHIIVLNNRQHNYEGNIYGVGR
                     EEGDIAGAPVYDSVEKIPEVPDVAIIIAPAETVPDFMDACGKKGIKRLVIESGGFSEY
                     SQGQNVLEKKVLEIADRYGMRFIGPNCIGTINFDIKMMMPFGFFPRTAPGGRVAMIVQ
                     SGGVGGSYLGEFGDNAIIPGKFAAIGNKLQIDEVDVLEYLLNDDKTDVVTMYLEGFKR
                     GRAFFDLAVQSDKPLIVLKSNRSDLTAKIAQSHTTALSADDQVVDGAFRQTAVIRVDD
                     DIDFINAVKIIRLPLMKSRRVAILSRSGGHAVMSVDACAKFGFSLVDFPPAFIENLKT
                     IYNTRVIAHQNPLDLGEIFDYTIFTQILEEALKLPDVDGVLFNHLYIADYEGKMSRDF
                     LSNVGKLVQQYQKPVAMTMVTDTQELVNISKNQSYPIFTTPLTAAKALNMSATYYEEK
                     TAREARGRAVQTTLDMAAIDIIQDKCKTQKRIPLTDESLRIVEAAGIKSIAGATVKSA
                     KEAGDVSLHFPVAVKLLSRDASHKSDIGGVRLNIKNQRELKKAISDIKKSLKEMKPRP
                     AMDGFLIQEMSADGVECFVGGRQDPVFGPIVIAGLGGIFLEIFRDTSIRLAPVTKSEA
                     LDMLKNLQAYPILQGARGKMKADIDAFADVICRVSNLLAAVPDIAEIDLNPVIVHEQG
                     KGVSIVDSRIFFK"
     gene            complement(12162..14393)
                     /locus_tag="PLG98_03155"
     CDS             complement(12162..14393)
                     /locus_tag="PLG98_03155"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOE32278.1"
                     /translation="MEQKHLAPFTPELTQNALTVLERRYLKRDKSGKILETPSEMFYR
                     VADTIAAADKKFNKKADTRKRAEEFYAMMASLEFLPNSPTLMNAGRELGQLSACFVLP
                     VGDSMDEIFDAVKFTALIHKSGGGTGFAFSRLRPANDVVLTTTGISSGPISFMRVFDV
                     ATETIKQGGTRRGANMGILRVDHPDIMDFIMCKADKKQLNNFNISVGLTEAFMTAVER
                     DENYDLINPRDKQVTGHLNARKVFNRIINQAWENGEPGIVFLDRLNRDNPTPQAGEIE
                     STNPCGEQPLLPYESCNLGSINLAKMVKEGKIDWARLKEIVYLAVHFLDNVVEVNKYP
                     LPQIAQMTMANRKIGLGVMGWADMLIKLGIPYNTDEAMELAEKVMKFINEQGHGASQQ
                     LARKRGPFPNFKGSIYDKKGQPPLRNATVTTIAPTGTISIIANASSGVEPLFAVSYVR
                     QVMDNDILVEVHPYFEALAKERGFYSPELMQRIAEHGTIHDLEEIPEDIRQYFVTAHE
                     ISPDIHIRMQAAFQKYTDNAVSKTVNFKKEATVQDVARVYELAYKLDCKGVTIYRDGS
                     RDQQVLSKGKKEETAVQEDADREQHLTKRERPKILKGWTYQMQTGCGPLYITINEDDK
                     GLFELFTTMGKSGGCAASQSEAIGRMVSLAWRSGLSSRQVIKQLQGISCHSPSGFGEN
                     KILSCADAVARAIQSHIEANGGKVQHEKIVFRKGACPDCGGIVEHEGGCAVCRMCGYS
                     ECA"
     gene            complement(14704..15582)
                     /locus_tag="PLG98_03160"
     CDS             complement(14704..15582)
                     /locus_tag="PLG98_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOE32279.1"
                     /translation="MTSTTMENLKKGKKALVVEGGGMRGVFAAGVLNAFGSGGFDPFD
                     MYLGVSAGACNLASHLAGQNDRNYDIIKRYSIDGRFINLGRFLRGGHLMDLDWLWDVT
                     ISEYRLDLKKLFGRLKEREKEYIIAVTSVATGKSAYLEPDEDTLEHYLKVSSSLPIFY
                     RNFLEVHSEKMTDGGVTDSLPVQEAIRRRATQITVIRTRPSQYIKKKSMFSMCYPIIF
                     RDYPLFAQAMKKRPGNYMESVRLIRHPPAGVRVFEIAPPPDIDVGRMTTDIKRLRAAY
                     QSGRDHGRKFMDAHAP"
     gene            complement(15616..15834)
                     /locus_tag="PLG98_03165"
     CDS             complement(15616..15834)
                     /locus_tag="PLG98_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32280.1"
                     /translation="MMAIIRKELKEKSKNVLLRVSIPAPLMAEIKKIKKLCRDTGFYF
                     DIKPDVISAVEQAVEEAKALIQKEKKSR"
     gene            16072..16284
                     /locus_tag="PLG98_03170"
     CDS             16072..16284
                     /locus_tag="PLG98_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32281.1"
                     /translation="MKEKHAIVKLVFGCIDDVVSTTESIHKSIAENVKGVSRSGFAEG
                     GKRKHVYDTIKAVNGKVEELVSGLKK"
     gene            16325..16744
                     /locus_tag="PLG98_03175"
     CDS             16325..16744
                     /locus_tag="PLG98_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HOE32282.1"
                     /translation="MSDNSRKKIGEEICMTSRRAKIGLGYHLAAFAAVNAVLVWINLD
                     TSPEYFWAKWPLAGWAVALSYHAFSVFSSLIKAHKGFYYHLFSFLIINAFLIFINFDL
                     YPQYLWFKFPLIVWTIMIVFHGWRVFSERQKAKAVAA"
     gene            16841..17809
                     /locus_tag="PLG98_03180"
     CDS             16841..17809
                     /locus_tag="PLG98_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32283.1"
                     /translation="MIKMKEIKAKNMNVNKFIDDKVRDIRQAVGDGFAINALSGGVDS
                     AVVTALGHKALGKRLKTYIIDNGIMRENEPAKVAAAFKKLGIQVDVFDASRAFFSALK
                     GLTDPEEKREALTQTFYKNVFGKLVVKSKARYLLQGTILTDVDETVAGIKRQHNVFEQ
                     LGIDPQKAFGYKILEPLIELRKDGVRKVGRGLGLPEDMFARIPFPGPALAARIIGEVT
                     PARIALVKKATAITEAALKDVKAFQYLAILHEDRVTGMRNNKRVFGNQIEIRCWNSLD
                     ARTARPTRLSYAILEGIAAKITNEIPDVVSVTYNITSKPTSTIEAI"
     gene            complement(17975..19870)
                     /locus_tag="PLG98_03185"
     CDS             complement(17975..19870)
                     /locus_tag="PLG98_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE32284.1"
                     /translation="MKRMIPLFLLLVCMTLAGCAGVKVSFVGSGDYMSLRRGDILTTG
                     ELSTYTGASLQVVGIDKKVCDKDASGCRKALMETIGLHSENRLSALAELWLKDAMELE
                     KQFIESGHIEATLNAYLETARYSYAYLFLTKRTPNMRALEDRQTQVRDYYNYAVEKTL
                     TTLFEYYRKRLPPDFNFEKDTHFESGQWHITIRNEDLRLAEEHIFPEDLIPASSLSFA
                     GLRNQYRRDGLGAELVAVTSTRVVSRQSAEMPFSETPFPPVTAVMSFPGNTWMDVLKT
                     NEVVITGYDPYSRESFKLVGKEVPLAANFTSGYGLWLARSGFAAQSLLTLIGKGDVLE
                     MPRVYLMQPYNPQRHIVIMLHGLASSPEAWINLANEVLGDETLRKNFQIWQVYYPTNL
                     PIAFNHYEISKAITQTLKHFDPEGKARASQKILLIGHSMGGILSRLMVSSSGDRLWGA
                     LLKKYPLEGRRLERVRRELGPYMSFEPLPQISRAIFVAAPHKGTPVAEIALVRWIANF
                     IKLPFSVLGRFREVAQLLVDPGSANPVSLTRSFNSIDNLSNRDPFIQLTSTLPVSSRV
                     RYHSIIGNNTPKKPLAESSDSVVPYSSSHLEGAVSEKVIASGHSVQEMPDAIIEIRRI
                     MHLHLKE"
     gene            complement(20099..21664)
                     /locus_tag="PLG98_03190"
     CDS             complement(20099..21664)
                     /locus_tag="PLG98_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32285.1"
                     /translation="MKNINVLMIQPKTPQTFWSHDHTIKALGFKATMPPLGLLTVAAM
                     LPQNFNVKLVDLNIEDLDMRDVDKSDMVFLTGMIVNRESFMEMVHICQKKGKIVVAGG
                     PFVQSVYGTKGSNDLEISKIDHLILYEAENNLPQFLDDYVQGKAKRVYDNNEKPSLTQ
                     TPIPRFDLIRPRDYVSMALQFSRGCPFNCEFCDIIQMFGHIPRTKTPEQFMGEVEALY
                     STGYRGRLFVVDDNFIANRKKVKELLRLLAAWQEERKYPFLLFTEASIDLAKDDELLG
                     LMRRSGFTSVFIGIESPDEKTLRATNKNQNVTCDMNAAIAKVQKAGIEVMGGFILGFD
                     TDADDIFDSQLEFIRKNSIVQSMVGLLAAMPHTDLYNRLEREGRLVLGVNHSGNNVDT
                     DLNFIPKMPVKQLIEGYRRVIKETYSPRNYFARASTLLKKFPDMRMSKLKYAEPWLKK
                     LYMSKMTSHPNKSKIVFEIMKLVFKPFGIYGLVFIIKSLRFGLLSMPVAIELTFRGLH
                     YMSVAREIENAAD"
     gene            complement(21814..22233)
                     /locus_tag="PLG98_03195"
     CDS             complement(21814..22233)
                     /locus_tag="PLG98_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE32286.1"
                     /translation="MVNKVILIGRLGKDPEVRYTPDGTMVTNFNLATDEQRKDKNKNG
                     EKIQKTEWHKIVTWGKLAEICGNYLVKGKLVYVEGRIQTRSWEDKEGVKRYTTEIIAS
                     DMRMLDSKGQTKSGETSFDADAANVNDGSVPLDDVPF"
     gene            22423..22746
                     /locus_tag="PLG98_03200"
     CDS             22423..22746
                     /locus_tag="PLG98_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32287.1"
                     /translation="MKNHENYVKGILEQNPDAEKLKELLAYHDKQIKWIQHERLVHLI
                     VMLFVCLFTMMIFGFAVIRPSLSVILLFVILLILSAAYLLHYFRLENGVQKWYVIAGE
                     IRRQF"
     gene            22784..23188
                     /locus_tag="PLG98_03205"
     CDS             22784..23188
                     /locus_tag="PLG98_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32288.1"
                     /translation="MTQNKIVAHYQDGNLFKGITNDFSPNKELFHLFERAAQEDNRPL
                     TLKMDGLKGLFYVKDYDGNPSYHEQKEFDPSRPLAGRKIKVIFKDGELMVGTTNGYQP
                     NRPGFFLVPADANSNNERCFVVSSAATEIAFI"
     gene            complement(23212..24576)
                     /locus_tag="PLG98_03210"
     CDS             complement(23212..24576)
                     /locus_tag="PLG98_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOE32289.1"
                     /translation="MSGVTTKETALATKLSKPEPRDETVPGSNAHGRFVSREIIALMG
                     KDDITSVSLGDHIEKTMTILFADIRGFTSLSEEMPPRENFTFINAYMKCMNPVIARHG
                     GIINKYIGDAIMALFPADADDAVKCAIGMLRQLVAFNRSIKKTGSSRIKIGIGLNTGL
                     MMLGIVGGEHFMETTVISDAVNLASRIESMTKDYNVPFLISEHTYYSLFGASEHNTRF
                     VDRVRVKGKAQCQSVYEVFDADPKSLRTAKQKTKADFEEALAFYHFKDVPKATKLLEK
                     LLRRVPGDTVAKVYHERCLRFLKTGIHESSGEFDLAISWNKSMAIDHSLIDEQHRDLF
                     IHTREFVEAVKTSKNYSQLRNVLEFMDKYIQVHFRTEERLMAQKNYPFLQMQVDQHRR
                     FSRYFDGLRNEIKRNFETNRIFILFRIQVLIVDWLINHTSKLDLHFGKFLRYRKGRGL
                     KSLS"
     gene            complement(24640..25623)
                     /locus_tag="PLG98_03215"
     CDS             complement(24640..25623)
                     /locus_tag="PLG98_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOE32290.1"
                     /translation="MKTAITEMFSVKYPIICGAMMWLCRPVLCAAISNAGGLGNLTAG
                     NYPTEDEFRDAIRQTRKLTDKPFMVNVTILPSVHVTAEHHKMYLRVCAEEKVAGLEIS
                     GTPLDKAVGKEFIDILKKAGVKLFHKVGSVRHAVHAEKTGYDGIYAAGIEEGGHPLND
                     DVTTMVLTPRIAETVKIPVVTVGGVADGRTLAAALALGAQGVMMATRFVATKECEVHD
                     NIKQEIIQRQEFETALICKSIGLQGRAIRNKLVEDVLAVESTGGGLEKLIPLIAGERV
                     KKAWEFGDVDSAPMMMGQSVGLVKDIPTCKELMERLVAEAQADIERVRNIF"
     gene            complement(25664..26389)
                     /locus_tag="PLG98_03220"
     CDS             complement(25664..26389)
                     /locus_tag="PLG98_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE32291.1"
                     /translation="MSIMNWTKDETVAIITMTNGENRQNLTFAKTLNAMLDEIIADKT
                     ITALIITSNDPKNFSQGIDVEWIMGQMQKNEHQPVTDFINEMDVVFKKLLLYPIPVIA
                     AINGHAFGNGSIIACACDFRFMRSDKGFFCFPEVNISIPFRWGMNAFVKKAIPIHLLS
                     QMQLTGNRFTAPELEKHNIIVKACANQEELMSSAIAFAKTFQKKRTIFGELKRRLYAD
                     IIKVLDDDFMPPTSKITSLVMMD"
     gene            complement(26636..27901)
                     /locus_tag="PLG98_03225"
     CDS             complement(26636..27901)
                     /locus_tag="PLG98_03225"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HOE32292.1"
                     /translation="MAERDMVVMSLGELKRLKLIQSAIDRQISQKQVSGILRLSERQI
                     RRLVRAVREEGDKGIINRSRGRPSNRGLSAGTKENVIALYKEKYSDFGPTFATEKLWE
                     RDGIRLSDETLRNWLIGAGLWKKHRKRSAYRQWRPRRSCFGEMVQMDGSHHDWLEGRG
                     PKLVLMGYIDDATNTIYAKFYDYEGTLPAMDSFKGYVKKYGLPMSVYLDRHTTYKSSR
                     KPTEWDEASDLKFLSQFERVLKELGVEVIHALSPQAKGRVERLFGVLQDRLVKEMRLH
                     GIKTKDEANGFLETYLPQYNEKFRVCPANETDVHVRPPRHFNWDKYLCIKDQRTIRND
                     HTIAYNRKLYQLEENDSKKVHIEKRRDGSLLIISKGRALKYKEITERPKQMVAVKKDL
                     RAYNRPPKPSKDHPWKQRWKTWGTTPTVL"
     gene            28108..29559
                     /locus_tag="PLG98_03230"
     CDS             28108..29559
                     /locus_tag="PLG98_03230"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HOE32293.1"
                     /translation="MGLKTADQYEESLRKMNLKVYLLGEQVKCPADHPIIRPSMNSVK
                     MTYALAQSPEHEDLMTAKSHLTGEKINRFCHLHQSTEDLIKKVKMQRLLGQKTGACFQ
                     RCVGMDAINAVDSVTFEMDKKLGTQYHDRFIRFLKMVQEEDLVVDGAMTDPKGDRSLP
                     PSRQTDPDLYTHVVEKRKDGIIVRGAKAHQTGAVNSHWILVMPTIAMTKDDADYALSF
                     VAPADADGISYIYGRQSCDTRKLEGCEIDVGNRQFGGHEALMVFDNLFVPWENVFMCG
                     EYEFSGLLVERFAGYHRQSYGGCKVGVGDVLIGAAALAADYNGAAKASHVKDKLIEMT
                     HLNETLFSCGIACSAQGHRTASGTYLIDLLLANVCKQNVTRFPYEIARLAEDITGGLM
                     VTMASEKDLRHPEIGKIVEKYFKGIASVPTEDRCRIMRLVENITLGTAAVGYRTESMH
                     GAGSPQAQRIMIARQGNLEHKKKLAKEIAGIKD"
     gene            29574..29891
                     /locus_tag="PLG98_03235"
     CDS             29574..29891
                     /locus_tag="PLG98_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32294.1"
                     /translation="MNCKISERKLIGFKYCGGCNPVINRAQLVQDIQRRLSAEFTLAT
                     DQSPTQWDIGILVCGCLSACADKPDFRNLARRWIIIAGNSVDYGDAPEKDLAEIVLNK
                     LKL"
     gene            30198..30899
                     /locus_tag="PLG98_03240"
     CDS             30198..30899
                     /locus_tag="PLG98_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE32295.1"
                     /translation="MTDKNRPKTRKDRIMDAALRIFAEKGFQNATITEISKAAGVSEA
                     TIYEYFGTKEDLLFAIPEKITNDTFEESSKVIPYIKGVEGKIRAILLFYVKLYQSNPN
                     YSALVLLQLMSNRRFRQTPAHAAIRTSAHTLLDCIREGIADGTFKKDSNPYLIRSVFM
                     GTIEHLFIHWHMQGMPKKKPGIAEMLDALLETVFDGIRARKEDSGTTLYLKLDDAGLL
                     EKILKKGRERHQNKK"
     gene            30936..32399
                     /locus_tag="PLG98_03245"
     CDS             30936..32399
                     /locus_tag="PLG98_03245"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOE32296.1"
                     /translation="MRTREQYIERLRKMRRNLYHDGEKIDRDDEIQAPVLNVMGLTFD
                     AVHDPELKDLCTATSHLTGETINRFCHVHQSTDDLHKKQDMTRALCRKAGMCIQRCMG
                     TDALNAVNAASFEADKVNNGNTQYHKNFLKWLERFQKEDLVGSCAQTDMKGERLKRPA
                     EQTDPDMYVHVVEKKKDGIVVRGCKLHISEASISDEIIVVPTRALGPDEKDYAVAFAV
                     PSDYEGVKQVVSIHNQHKRKHIKRGIDIGYSDSYIIFEDCFVPWERVFLCGENDHGGM
                     CALLFALFHRHSYSGCKPAIGDVFLGLAALAAEYNGIEKTPHVKEMLAEIIKVSELGY
                     AAGYTASSMGKPEVIIPGLGMCPYGPGSYIPNSIYCNVGRCLTGEAVFHEQELICRIA
                     GGLPSTFPYEGDLTHPDLKKVLEKYLNRNPKVPVEDQIKFWMTLGDYTIGTMAGVMNY
                     GNYHGGGSPIMEQIAITSQYDIKDRKKLIKRLAGIGG"
     gene            32511..33311
                     /locus_tag="PLG98_03250"
     CDS             32511..33311
                     /locus_tag="PLG98_03250"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE32297.1"
                     /translation="MLDVSQFSLKDKVAVITGGGRGIGQAIAFAFARAGAKVVVTSRK
                     APDLEATANEIKAFGGDAYPLPAHLGKSEEIKKMIDAVMAKYSRIDILVNNAGASPAK
                     GSVLECDERLWDKLMDINLKGAYFISQAAANIMVKQGGGKIINIASIDGHNPEPGLSI
                     YAISKAGIRMLTKAFASELVRYNIQVNTIAPGPIATKMLKSNWEFLPPEDAQKVKELV
                     EKALPSGRIGDPDEIAGAALYLASDASSYTTGAEIVIDGGVLLGMHPS"
     gene            33326..33865
                     /locus_tag="PLG98_03255"
     CDS             33326..33865
                     /locus_tag="PLG98_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOE32298.1"
                     /translation="MISRTAAVLVIIDIQGNLAQAMFGKENIFANAVKLIKAFKTLNL
                     PIIVTEQIPGKLGKTLPQIADEIEDFNPIAKESFSCWGEKNFKERLESLSRRHVVLLG
                     IETHVCVYQTALDLLSEGYNVHLVADAVSSRTPENRQIGIKAMKSAGAHIASTEMVLF
                     ELLRTAADPKAKEFFKIVK"
     gene            complement(33983..35914)
                     /locus_tag="PLG98_03260"
     CDS             complement(33983..35914)
                     /locus_tag="PLG98_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HOE32299.1"
                     /translation="MFSAHSENESATESPGEPPSGGFNKKIIFLAFASIGVVFGDIGT
                     SPLYAIKACFYGQHALAPDITNILGVLSLIFWSMFVIVSIKYVIFLLRADNRGEGGIF
                     ALVSLLNVSKKSFSPLVQSVLVLAGILGAGLLYGDGVITPAISVLSAIEGLEVATHAA
                     APFILPLTCVVLILLFVLQRKGTSNIGKVFAPIMVLWFLSIGIFGIMQIVREPSVLRA
                     LNPLYAVEFFLNNGMHGVVVLGSVVLCITGCEALYADLGHFGRNAIRLSWFSLVFPSL
                     LCNYFGQGALLLAHPEMNVNPFYKIVPEILLYPMIGLSTAATVIASQALISGAFSLTQ
                     QAAQLGLCPRVRIIHTSSDMQGQVYIPSVNYALMMACIAVVTGFRESGALAGAYGIAV
                     TGTMIITSILYFLVLTHHRGWPLRKVIPLVGLFLLVDIAFFTGNFFKLADGGWLPLLI
                     AAIIAVAMTTWKKGRNELYRNLVGARFPLESFLSELPKRHMPRVPGTAVFMTLSPVGT
                     PPTLLHNVKHNHILHETVVLLSIITKDTPAVPAGERIKLADLGQGFYRVEAFYGFMQK
                     PNVPQLMKILAQYGLVTDPMTTTFFLGKETLLTGGKSKMMKWRKSLFAFMSRNAGNPT
                     SYFGIPANRVVELGTQIEL"
     regulatory      complement(35969..36099)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            36402..37673
                     /locus_tag="PLG98_03265"
     CDS             36402..37673
                     /locus_tag="PLG98_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOE32300.1"
                     /translation="MAQAQVFENKQPVLGINSLGRIGKLTLWHHIGRKYFKEIIVNQG
                     REVGTGLDSVAQAIEKDSTYGLMHKFLYGVKAERIIQIVDEKEGKLLIDGIPVTILRE
                     ARNPADVPWKKYGVDVVVDCTGKFKDPTVAADHKSGSIRGHLNGGAKVVINSSPFKIK
                     NKALALPDDAVTMIYGINHTSFNPKKHLIVSAASCTTTGLAHMVKPLLENEETSNILT
                     ASMSTVHAVTNTQSVLDSVPKAGEKDLRKNRSILDSIILTSTNAAKALAEVIPQVQNI
                     GFMADSIRIPASTMSLIVLNATFQARSQGAATAINTKMLNELYGKAAANNPEKLIRFT
                     MEQNVSADLFGIDAAVIIEGQFNHTRTAFINLDLAHIPNMPAEVANSFPDKVMKVPVV
                     HAKIFGWYDNEYGSYTNRMGDLTVHIHKNIL"
     gene            complement(37752..41969)
                     /locus_tag="PLG98_03270"
     CDS             complement(37752..41969)
                     /locus_tag="PLG98_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOE32301.1"
                     /translation="MATTKDNPRKKSEELDSFALKVNLERTAATIEIPDQYAPLLKVV
                     ENHYGLQKKIYELLTELHHPFVNWEYVLKELKSVSIGDFYIYNKHQDGLSALATMLCI
                     YFEVIKSSSDKDVKDNAVHYLFDYIDTILNQSNEQTARNLLLFPEIIKSLLDAINQQE
                     IIFKKCSSYAKKIIKNFPGHGDVLQNRLFVDLLYRIFRITYQFWLEQPDPSQWFIAGE
                     GESPESVAAYGQFIRPLSHASMRMLMEKLETSYQNTHEDKNDAIKNYLEMPDYFQIVN
                     GYLQIADQLEKSKTYEGHQHITKLRFLFQVMEVPSLADIHASALREINHSLKMVFQEE
                     QQENLHDFVRKIFGFLKKSKSQREFSGAIFDCITITAREVFAQNSHELANTFISELIA
                     YGFQYPEIKGSNEEWQVKVNPAHIMNIRAWLEIISMKPRWTKKLISALVINLKMGGIF
                     IRDTDLIQKNISALLNTDIAPAYSLIKQLLRIFPVYFSEIGAEGELRDITTKVDELSM
                     RNDKLVYFFRKQSHVESNSLLVKFTEDIFQYWNTGDMSYLRSHLPVEVYEQVMNPGEY
                     FTGMHKAFKYFFTKVQSPLKFLDWDNEKVCKELANVKGINARDQERVKIMIRIYQLIY
                     KKYYPQFIDLLKDLEAVNAFPKADISSLKRSLTTKNYHRSITIILKFLSILKERILSP
                     KKTPSYENIYYKRHIAAGIPSMYGTYYERKFEAVGLSLRLETLATMLFEELSKSFNLK
                     FITKHTIRKIHTYLWLYMNALEIEGIATEGLAAKIKYVTNALSIKQFSLEQYVDIFRF
                     ISKDIQDIIRNYYIDIHTTCLPVVIGQVYRREIDSERKSGQKQHEKLIYAQSENFIRG
                     IIASAFGIQVLDNFVNSVIKTLNAESEKFRDNKQILKVVMAYNPELAVSSFYKKNKKV
                     DNQILLGNKGYFLKELVSFGFNVPPGFIITTEVFRGYEAVYGYKYIFNDLAVRVNKEI
                     KKLEKITGRKLGDRRNPLLLSIRSGATVSLPGMMKSFLNVGINETIAENLSEKDGFKW
                     AAWDSYRRFLQMWGMFKGLNRNFFDAIIDEFKQKYGVERKIQFSPEQMKQIALSYKKG
                     LTDNGIEFKEKPLDQLRDAILQVFDSWYSEQAEIYRRQMHISDKWGTAVIVQKMVFGN
                     LNDNSGSGVIFTREPKSTSSDVTLYGDFIFGVQGDDIVSGLVETYPVSEKQRIAECRD
                     SKISLEDKFPEIYAELLRISEILIYEKGFNHQEIEFTFEGPTRDKLYILQTRDMNQIT
                     TQNWRRFKDSPALQSSMIGAGIGVNGGALAGRAVYSEADIKRFRSEEPQTPLILVRPD
                     TVPDDVGVLLQVEGLLTAKGGSTSHAAVTIPQLNKVGVVGFSKLKVYETDEYAAIENQ
                     TIRAGDFISIDGWSGTVYLGRHESETE"
     gene            complement(42166..42477)
                     /locus_tag="PLG98_03275"
     CDS             complement(42166..42477)
                     /locus_tag="PLG98_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32302.1"
                     /translation="MSRAKVGDVLGCEVCGLKVILDEDCGCATIDLICCEEPMVNLGP
                     AKPKKAQAIAAPRKKTVVKKTKAAPKKKPALKKAKKPTVKKAVKKATVKKTAKKSSKK
                     K"
     gene            42751..44703
                     /gene="alr"
                     /locus_tag="PLG98_03280"
     CDS             42751..44703
                     /gene="alr"
                     /locus_tag="PLG98_03280"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOE32303.1"
                     /translation="MPHKKNQKYRSWVEVDLDNFIANLKEIKRLIGAKADFMMTVKAD
                     AYGHGAIEISNIALKCGARMLGVANADEGVQLRISGIEAPVVILGPSTTAEIEQIIKY
                     NLIPSVSDLSFAKKFNNALIKSHHKRPLHIEVDTGMGRGGAMHTEAVKLVLDLSGLSN
                     IILEGIFSHFASSEKMVSHNSTQWKCFSDVLAEIESRGIYIPIRHINNSGAILNYPEY
                     KLNMVRPGIMAYGIYPSPENESKAGLLPVMSFKTGIVLLKDFPAGHGIGYNSTFVTSR
                     PTRIATIPVGYGDGYPFILSNQSTALIHGQRAPIVGRISMDMCTVDVTHISNCKIGDE
                     VVLLGQQEKECVSANEIAAKAQTISYEILCALGKRAPRVFLQKGKHKTVEPRLKRIFV
                     PEEERSLSRIDNIIRHCLLARARNEELGNAIYYEMFETLFGNRDRRLELRSGFRYDIR
                     IAKMPKTKDVVLSKDYLHVGMHVQYKKTLRDNIFMIGCASSHSQLNALFKDPRCEYRW
                     LLNDGGNLSGKRDFTVERVRIDNEDVPIIETKSTKRGYEVWCGSDRLKKKINQDVKLE
                     MDISTKQARQKRTFPVYLVYPVRGLEIKFDYANAGIKNVREESFFAGKHPQPSVTGKK
                     GKSIAVKISDEEWIFPTSGVIFIWDD"
BASE COUNT        11725 a        11029 c        10809 g        11300 t
ORIGIN      
        1 gcaacgtatc ccttttatgc aaaccttcgg taacatttta tttgaggcaa ttcgtccatc
       61 atttgaaatg ttgatatatt ttcttattat tgtataatca cgcagtatga ttaaagagta
      121 tcatgtaaat gtgtaatgta acgctgtttc ccgttccggc accccaaaca ccggccattg
      181 cacaatccga ctctgaacct gtaaccgcag aagcaccggc aattgttcca gagggaaaga
      241 aggtcaacaa aataatcgtc gcgaagttgt tcggaagcga acaggttaat taaatcgttt
      301 tcattatttc gagcattgaa atcataagga ggttacagaa atgaatattg gcgaacttct
      361 aggagcaatg gtgcaaaccg gtatgtcacc gtcttccagc gcacgcatga agaattctct
      421 tggaggcggc aaggaactgg atagtctcaa agatatgtta ggcgatgccg caggacagtc
      481 cggcagcgga ggactcggag gcttgctttc cagtgtgctg ggcggagaca aaagctcagg
      541 cggcaaaagc ggcggtatcg gcgacttact gtccggtgtg ctgggtggcg gacagaaggg
      601 aggcgcatcc agctcaggcg gtatcggaga tttactttcc aatatgttgg gtgatgccgg
      661 taaggcagtt ggcggcaaca aaaacctggc catcggcggt ctgggagcgc ttatcggatc
      721 cattatgggc ggaggtggaa aatctgcagg cggcgccgtg ggcggaggct tgatggctct
      781 gctcggagcg ctggcgtata atgcgctcaa aggcatcggt caggccaaga ctcccattcc
      841 agccggcctg attgaacccg gtacggatga ggaacaaaag caattggatc agagttcaga
      901 actggttttg agggcgatga ttaatgccac gaaagccgac ggcaagatcg atcaaaatga
      961 aatcaatcgg atcatgggaa aattcaatga gattggtgtc gaagaggaag gaaagaaata
     1021 tctgatgatg caactgcagc aaccgatgga aacggacaca ttgattgccg cggcacaggg
     1081 gcagccggac ctggcggcgc agatgtatgc ggcttccctg atggccatag aagtggacac
     1141 gcagaatgag aaagactatc tggaaaaact ggccgaaggt ctgggattag gctctcaggt
     1201 caccacgcgt atcgaacaaa tggttggttt acagccgtca taaatattcc gggcgacgaa
     1261 atggattgtg cctctctgtt tcggtcaccc ttttttattc tgaatggttg tcatcgcatg
     1321 ataaacattt cttgcgattt gatttttctc gttgttcctg cccacctggt gtgtgcgcca
     1381 actctacaca ccagacaaca gacatttcgc tttccggcat cgtgattttc agatggttga
     1441 gattgaccga atgaacacaa atgtgtaaag tgctcaagca aaaatggaga tccggcgttt
     1501 aatgatcaag acaattttct gggacaatga cggtgttctg gtcgatacgg aaggcctttt
     1561 tttcagggcc aatcaggaaa tcctggcagg tttgggaatt cccctgcaac gggagcaatt
     1621 tgaagaaata tcgcttgtca ggggagaaag cgtgatgcgg ttggcggcaa ccccggggac
     1681 ggatgcgttt atcaaacttc gccgggatcg tgacgccctt tatgccgatt tgctgacccg
     1741 cgacccgctg gtgattgacg gcgtccggga agtgctggat gcgcttcagg gaaaatattt
     1801 gatgggtatc gtcacgagtt ccggacatga gcactttgaa atcattcacc ggcaatcgag
     1861 tcttctgccc tatttccagt ttttcctgac cctgaaagat tgcccttata ccaagccgca
     1921 tccgggaccg tatctcaagg caattgcgtt aagcggtttt gcgccggagg aatgtctggt
     1981 cattgaggat tcgccgcgcg gtctggccgc cgccacaagc gccggactcc ggtgcattgt
     2041 catccccagc aagttcacgt gcaaccacga tttcgcagga gcctgcaagg tcctggacag
     2101 catcaaaggc cttcccgctt ttctcaaaga attttaaaaa gtcagtatca atgctcatca
     2161 gcctcttgac aaactctttc ccatcggata ggcaactaaa aggggtggcc ttaacggtct
     2221 gagattatac cctttgaacc tgatccgggt aatgccggcg taggaagaaa aaaggcgtat
     2281 tgatccgttt cccctgatga ggaaacggat tttttttgga tatccagccg gcaagccgcc
     2341 caggttaatc tttgggaggt agtcatgtta aacgaaatca ttatcagtcg ggcaattctg
     2401 gattcctatt ttaaaaagct gggcagctgt ctggatcttg atgttgccat cgtcggtggc
     2461 ggcccgtcgg ggctggtggc cggatactat ctggccagag cgggacggaa ggtagctctt
     2521 ttcgaacgca agctctcaat cggtggaggc atttggggag gcggcatgat gttcaattcc
     2581 atcgtggtac aggaggccgg acggcaattg cttgaagaat tcgacctcca gggccgggaa
     2641 tatacgccgg gctattatgt ccttgatgcc gtggatatca cgtcaacgct tattcacaag
     2701 gctgtgcggg ccggtttgca ggtcttcaac ctcatcgcca tagaggatgt ggtaatcaaa
     2761 aacgaacgcg tggccggtct ggtcattaat tggggcgcgg ttgatacgct aaaatggcat
     2821 gtggacccgc taaccgtcca tgcccgatat gttatcgacg ggacgggcca tcccgccaat
     2881 gtcaccgagg tgctcgtacg gaaaatgggc gtgagactca acacaagcac cggcgggatc
     2941 gtgggcgaaa aatccatgga agccggaaag ggagagttgc aaaccgtcga aaatacccgc
     3001 gaggtttatc cgggactttt tgtcagcggc atggccgcta acgcggtcta tggcggctac
     3061 cgcatgggtc cggtcttcgg cggcatgctg ctctcgggac tcaaggtggc tgaagaaatt
     3121 ctagcgcgct tatgaatacg gcagagggac aaataatgcg cacattcctg cggcacgggc
     3181 tttacctgat cctcaccgat ccgcgcgacg gttatgaaac gctttgccgg tgggctgtgg
     3241 aagccggtct tccggccgtt cagttgcgct ataaaggtca taatgaacga gaacatcttg
     3301 ctctggcacg tgccctgcga caaatcacca aaggtaccgg cacgctcttc atcgtcaatg
     3361 accggccgga catcgcactt atttcgcaag ccgacggtgt gcatttggga caggatgacc
     3421 ttccggcagc agaggtgcgg cagctcatcg gtccgaccat gctgctgggt ctttccacac
     3481 acaacctaga gcaggtcgcg gccgcaaatg ccgtacccgt ggattacatc gggttcgggc
     3541 cgctgtttgc caccaattcc aaggaacggc tggcaccggt gacgggaccg gatatcttga
     3601 aggccgcgct ggccatcagc cgttatccca tcgtggctat cggcgggctg accctggagc
     3661 gtatcaggca attacaaccg cgcccgcaca atacggcagt catccgcgcc gtaaacgatg
     3721 catccgatcc gttggccgtt atgcgcgcca tccatgaatc gtgtatgaac aggacatcat
     3781 aatgtttttt atcgttggcg ctgcagattc cctttgtcca ttgtcttcag atattctatt
     3841 cgttcaataa gcggcggatg ggaataataa aaccacacat accacggatg gggatgaaga
     3901 ttggacaggt tatctttggc tagacgtttg agggcatgta tcatcggctc tgacgttccg
     3961 gtcaattcat gtgccatcct gtcggcttcg cgctcatatc ggcgcgagat gaacgcggcc
     4021 agagggctga agaagaatcc gacgcaactg aaatacagtg acaccagaag caaaccggca
     4081 taaacaggcg tttgcggtaa accgaaggcg ccatagatcg gcggccagtt taccagcaga
     4141 taaaccagat aaaatccgac aaaggaagcc gcaaccataa atattaattg cttcaggata
     4201 tgtttctttt tccagtgacc gatctcatgg gcaagcaccg ccagtatttc atcatgcgta
     4261 tgtgaagcaa gcagcgtgtc gaaaagtacg atgcgcttgg ttttgcctat acctgtaaaa
     4321 taggcgttgg tatgtttgga acgcttacct tcatcaacct gaaaaatgcc tttggccttc
     4381 aatccgactt tggaaagtag tgatgtgatt ttctctttca gttcttcatc cttgaccggt
     4441 tcgtacttgt taaacagcgg cgcgatgacc acgggaaaaa gccacaaaag caggatctcg
     4501 aacgcggcaa aaaatatcca tgcccagaac caccagcttg tcggcaggta aagaacaaaa
     4561 gccatgaaaa cggaaagtaa aaaccccatc agaattgctg aaatgatcac ggacttgacg
     4621 agatcggtca gccagagctt ccatgtaatc gtggaaaagc cgtatttttt ttccagaaca
     4681 aacgtgcggt aaatgtcaaa gggaagtccg ataacgccgc caatcaacgc cagagttccg
     4741 aagaaaacca gcgcctgcca gatcaagtga agctgcaatc cgtttgtttt tgcaaccagc
     4801 agcggtagga aaagaaaaat gatggcaagt tccagaaggt cttctgtgga attttccttg
     4861 ttctccagac gcaaattatc atacgtataa tccgccattt tggaaagcgt tgcctgatca
     4921 atcacattcc ggaaagccgg cggaataatt tttccgcact ggcgcaaatg ccggatgttg
     4981 agttgctgca aaataaatcg ccagaggatc cggccgacaa acaaaattaa aaaaattatc
     5041 agaagagttt tttccataac cgtctctttc gtccgtcaga ttgttttcgt tacatcatcg
     5101 tattttaaaa ccacagcctg tgcataaaaa atatgccgtc agatgttcag acggcggcga
     5161 agttttttca tcagcatcat accgcctgaa aaccaggttg tgtcaaaatg atgaatcgta
     5221 taggacattt caactgcctt gcttcgttcc aattcggaac acgcttcgtg ccaggacagc
     5281 ggataaaaca cctcatacgg ataaatcgtg atgtcctgat gcgtttgctg atagtctctg
     5341 gccagtttgt ccagctcaaa aatgacggtc tgtatgatgt aatggccttt ggattgcaat
     5401 agccttttgt gacggaatgt cacccgaagg cgctcaagca gcatttccca gaacggatgc
     5461 cccttcggcg aggccataaa ggcattaatg atgaagtcac acctgttgag ggacttcccc
     5521 atcccttctt tttcttcccc cagaatcaga tgaggttgat cgatgatccc gtccaggggg
     5581 cggagcggtt cgagatccag atcggcataa acaccgccaa atttgtgcag gtataaaatt
     5641 ctggcaaaat ccgctttttg aaccggatac gcataggagt cgtaaagttg cagaaaagcc
     5701 gtataatctc tttccaccaa acgacgattg tcttcgtccg tccaaagcct gaattcccat
     5761 cccggatgaa attttttcca tgcgtccctg aatctttgcc agcgcggagg gatctcggtt
     5821 gatttccagg tctgatggat tatttttggt atcacagcat tgtcctttct gaactgaaaa
     5881 cagcaataaa aaaccgcagc ataaaatgaa aatatttatt ttttgcggct aaaagatagg
     5941 taacatgcct gtctgtgtca aggaaattgt ctgcgaggat aaattcttac gttacacccc
     6001 aaaaagtctg caacgggaaa caaaccagca aactcaattt caatccaaac gtcctgtccg
     6061 aagatctgct cttgaatctg tacagcaaaa gcgtgtccgc cggctgccga ttaaaaaata
     6121 aaagattttc ttaccgtata aaaaactata aaattgcctt gacaaaatca tctgccggtc
     6181 atatcattcc agttaacgta agctggactt aacataaaaa acgatcgtaa tattatagta
     6241 ttatgtccga tagtctgttt gcgtaaagga catgaagtag tttgatcata caaaagtcat
     6301 caaacgtctt cgaggaggta ttaaatgtca aattttgtga agaaaacaat cggccaactt
     6361 gtcagggaaa cagccgagtg ctacccgcaa aacccggcgc ttgtctgtcc ggatttcggc
     6421 attcgtcaga cgtacagtga attttaccag acgtgccggg acgtcgccaa agggcttatc
     6481 gccctgggaa tcaaacgcgg ggataatgtc gccgtctgga caaccaatat tcccgactgg
     6541 attcatctgc aatttgcgct gggaatgacg ggcggcattc tggtaaccgt caatacaaat
     6601 tatcagacgc acgaactgga atacattctc aagcaatcgg attccacgac gcttttttta
     6661 atggagtcct atagagaagt taatttttac gaaaacgtcc gcaaaatatt gcccgaactg
     6721 gatcaccacc agccgggaac cccggtttcg gcacaactcc ccgctctgaa aaatattatc
     6781 tacatcggca agcgggaaag caccccgggc atgctgcatt tcagcgacat catcaccatg
     6841 ggcagggagg tgaccgataa acagctggag gataggctcc acagcctcca tgatgacgat
     6901 gtcatcaata tgcagtacac ctcgggcaca accggattcc ccaagggtgt catgctgacg
     6961 catcataaca tcgtgaacaa tgcacgcatg gtcggcgacg tcatgggcat gacggaaaaa
     7021 gacaagctgc tgatccaggt accgctgttt cactgtttcg gctgtgtaat gagttcgctt
     7081 aattgcgttt atcacggctc caccatggtc gtcgtggaat atttcgatcc cctcaaagct
     7141 gttcaatgga tttccgcaga acactgcacg gccgtaaacg gcgtgccgac catgttcatc
     7201 gcgattctga atcaccccga tttcgacaaa tatgatatgt cttctctgcg gacgggcatc
     7261 atggcgggcg ctccctgtcc tgtcgaaacc atgaaccagg tgcgcacgaa aatgcattgc
     7321 cccgaaattg tcatcgcctt cggccagacc gaatcggcgc cggtcatgac catgacccgg
     7381 cgcgacgatc ctgtggaatt gcgcgtctcc accgtgggac ggcttctgcc ggatatcgag
     7441 ggaaagattg ttgaccccga tctcggcatt gatttgcctc ccaatgcgca gggcgaaatt
     7501 gtcacccgca gcgaatgtgt catgaagggc tactacaaaa tgcccgatgc cacggctgac
     7561 gccatcgacc gatacaactg gttacacaca ggcgatctcg gagagatgga cgataacggt
     7621 tatttcaaag tgaccggccg tattaaggat atgattatcc gcggcggaga aaatatttat
     7681 ccgcgtgagc ttgaggaatt tcttttcacg catcccaaag tcgtaaacgt tcaggtcatc
     7741 ggcatcccgg ataaaaaata cggagaacag gtcctggccg ccattcaact caaggccgga
     7801 cagacggcca cgcctgaaga attcaccgag ttttgtcagg gaaagatcgc ccggcacaaa
     7861 attcccaaat actgggagtt tgttgacgac tatccgacaa cggccagcgg aaagattcag
     7921 aaattcaaga tgaaggaagt gtttgaaaaa aaatatcctg cttaaaaact atcagggaga
     7981 aaggtaagaa aatgagcaac atcatcaacc gcgatactat cggcgacatg gcgaggaggg
     8041 cggcgacaaa atacggcgat aaaaccgcca ttattttccg caatatcaag ctttcattct
     8101 acgatctgga acgggaggcg cggcgctttg ccaatctgat gacccgctac ggtgtccgga
     8161 agggagaccg ggtcgccgtt tacgcctata actcccatta ttatccgatc agcatgatgg
     8221 gactggccaa aatcggcgcc atccaggttc ccatcaatta catgctcaac gcggaagaaa
     8281 ttgccttcat catcaatcat tccggttccc gtttattcat catcgaagat acgctctatc
     8341 cggtcatcgc cggatgcaag gataaattcc ccgccgtgga aaaatgggga ttcattccgc
     8401 tgaatgattc tggcattccg gacgggttct tcaatttaat cgccgagatg tcggcgatga
     8461 attatgacga accgcaggtg gaggtcagcg cagaagatat cgtgcagatt ccctacacca
     8521 gcgggacgga ggccaaaccc aaaggcgcca tgctgtctca cagggcattg atgtcgcagt
     8581 atcacagctg catttttgac ggccggtatg agactgacga cgtcagtctc cacgccctgc
     8641 ccctgtttca ttgcgctcag ctccattgtt tcctgatgcc ttatctgtat gtcggcgcaa
     8701 ccaacgtgat catgcataag gccgatcctg tggagatgat ccggctgatc gaaaaatatc
     8761 agattacaca catgtttgct ccgcccaccg tctggatcgc catcctgaat catccggaat
     8821 tcaacaacta caaccacggc agcctgaaaa aagccgccta cggggccagc atcatgccgg
     8881 tggaaatcat caaacagttg tccgtcacat ttaaaggctt gaagctctgg aattactatg
     8941 ggcagacgga gatggggccg gtggcaacca tcctgaagcc cgaagatcag ctcctgaagc
     9001 ccggttccgc cggcaaaccc gttttaaatg tggaaacccg gctgatggat gatgacggaa
     9061 aatttgttgc actgggcgac gtgggggaaa ttgttcatcg ctccggtcat gtcatgtccg
     9121 gttatctgaa tgatccggag aaaacagcgg aagccttcca gttcggctgg ttccacagcg
     9181 gcgatctggg acgatttgac aacgacggct atctctatgt cgtcgaccgc aaaaaagaca
     9241 tgatcaaaac gggcggagaa aacgtcgctt cgcgcgaagt cgaggaagtc ctttatcagt
     9301 atccgggcat cgaagaggtc gccgttatcg gcctgcccga tcccaaatgg atagaggttg
     9361 tggcggtggt cgccgtcctg aaaaagggtg tgtcgattca ggaaaaagac attatctcat
     9421 tctgcaagga tcgtctggcc ggttttaaat gtccgaagaa agttttcatc accgaccggc
     9481 tgcccaagaa tccttcggga aaaattttaa aacgggagct gaaagaacag ctcagctcat
     9541 caatttaacg gggggtgaat cgtatgcaag acacgcaaga aaccttcacc tctatcggag
     9601 aaatcgctaa gaaactggat atgagccagc gcactatccg ctattatgaa gaaatcggcc
     9661 tgctcaattc catcaagcgc gttgaggggg gacgccggat ttataccgaa gccgatttcc
     9721 gccgtctgaa actgatcaag cgcctgaaga tcatgggcat gacattatcg gagatgcagg
     9781 agctggaggc catgtggacc attgaaaaat ccaacgacaa ggtgctcgga cgcctgctgg
     9841 agcttttgaa taaccagttg cgcagactgg acgaccgcat cgccgatctg aatattttaa
     9901 gaaatgaaat tgtggaatat caaaaaagga ttaaatccaa aatcggcaaa tgattcttgg
     9961 agattccgaa ttgtgtaact agggaggaac cgcacagaaa aatggaaaag tttttttatc
    10021 cgcgcagcat ggtggtcatt ggggcatccg tcaaacggct taatctgggt catattatcg
    10081 ttctgaacaa ccgtcagcac aactacgagg ggaacatcta cggcgtcggc cgtgaagaag
    10141 gcgatatcgc gggtgcgcct gtttacgaca gcgtcgaaaa aattccggag gtgccggatg
    10201 tcgccatcat cattgctccc gccgaaacag ttccggattt catggacgcc tgcggtaaaa
    10261 agggcatcaa gcggcttgtg attgaatcgg gcggattcag cgaatactcg caaggacaaa
    10321 atgtcttgga aaaaaaggtg ctggaaatcg ccgaccgcta cggtatgcgt ttcatcggcc
    10381 ccaactgcat cggcaccatc aatttcgata tcaaaatgat gatgcctttc ggcttctttc
    10441 ccaggacagc gcccggcggg cgagtggcca tgattgttca aagcggcggc gtgggagggt
    10501 cctacctggg cgaattcggc gataacgcga tcatccccgg caagtttgcc gccatcggca
    10561 ataagcttca gatcgatgaa gtggatgttc tggaatatct gctcaatgac gacaaaacgg
    10621 acgttgtcac catgtatctg gaaggattca agcgcggcag agcctttttt gatctggcgg
    10681 tccaatccga caagcccctg attgttctga aatccaaccg cagtgatctc acggcgaaaa
    10741 tcgcccagtc ccataccacc gccctctccg ccgatgatca ggtggtcgac ggcgctttcc
    10801 ggcaaacggc ggtcattcgc gtcgacgatg atattgattt catcaacgcg gtgaaaatta
    10861 tccggctgcc attgatgaaa agcagacggg ttgcgatcct gtcgcgatcc ggcggacatg
    10921 ccgtgatgag cgtggatgcc tgcgcgaaat tcggattttc gcttgttgat tttcctccgg
    10981 cttttattga aaatctgaaa accatctaca acacgcgcgt catcgcccat cagaatccgc
    11041 tcgatctggg agaaattttc gattacacca tttttaccca aattctggaa gaagccctga
    11101 aactgcccga tgtggatggc gttttgttca atcacctcta catagccgac tatgagggga
    11161 aaatgtcgcg ggatttcctg agcaatgtcg gcaaacttgt acagcaatat caaaagcctg
    11221 tggccatgac catggtcacg gatacgcagg aactggtgaa tatttccaag aatcagagtt
    11281 atccgatttt tactacgcca ctgacggccg ccaaagcgct gaacatgtcg gccacctatt
    11341 acgaggaaaa aacggcgcgg gaggccagag gcagagctgt tcagacaaca cttgatatgg
    11401 cagcgatcga tatcatccaa gacaaatgca aaacgcaaaa aagaattcct ttaaccgatg
    11461 aatccctacg catagtcgaa gccgcaggca tcaaaagcat tgccggcgcg acggtcaaat
    11521 ccgcgaaaga ggccggagac gtttctcttc atttcccggt tgccgtgaaa ctgctttccc
    11581 gcgacgcgtc gcacaaatcc gacatcggcg gcgtgcgcct gaatatcaaa aatcagcggg
    11641 agctcaaaaa agcgatttcc gacattaaaa aatctctaaa ggaaatgaag ccgcgaccgg
    11701 ccatggatgg tttcctgatt caggaaatgt ctgccgacgg cgtggaatgt tttgtcggcg
    11761 gacgacagga ccccgtcttc gggccgattg tgattgcggg actcggcgga atttttctgg
    11821 aaatcttcag ggatacgtct atacgtctcg ctccggtaac gaaaagcgaa gcgttggata
    11881 tgctgaaaaa tttacaggcc tacccgattc tgcaaggggc cagaggaaaa atgaaggcgg
    11941 atatcgacgc ttttgccgat gtgatttgcc gtgtgtccaa ccttctggca gcggttccgg
    12001 acatcgccga aatcgatttg aatccggtta tcgtccacga acagggcaag ggcgtttcga
    12061 ttgtcgatag ccgcatcttc ttcaaataag aacgtgacag tggaatagcc cggcaatgca
    12121 ttgttgatga cggtcgttga cgcatcgtcc cgatgcatcg gttatgcgca ttcgctgtaa
    12181 ccgcacatgc ggcaaacagc acaaccgcct tcatgttcca caatgccgcc gcagtccgga
    12241 caggcgcctt tgcggaatac gattttttcg tgctgcactt tcccgccgtt ggcttcgatg
    12301 tgggattgaa tggctctggc cacagcgtcg gcacaggaca gaatcttgtt ttcaccgaaa
    12361 ccggacggcg aatgacacga aatgccctgc aactgcttaa tcacctgacg ggacgacagt
    12421 ccgctgcgcc aagccagaga aaccatgcgt ccgatggctt cgctttgtga tgccgcgcat
    12481 ccgcccgatt ttcccatcgt ggtgaaaagt tcaaacaaac ctttgtcgtc ttcgttaatg
    12541 gttatgtaaa gcggtccgca accggtttgc atctgatagg tccagccttt caggattttc
    12601 ggacgttcgc gcttggtcag atgttgttcc cggtcggcat cttcctgcac agctgtttct
    12661 tcttttttcc ctttggacaa aacctgctga tctctggatc catcgcgata aatggttacg
    12721 cccttgcaat ccagtttata ggccagctca taaacccggg ccacgtcctg gacggtcgcc
    12781 tcttttttaa agttaacggt tttactgacg gcgttatcgg tgtatttctg aaaagcggcc
    12841 tgcatcctga tgtgtatgtc cggcgatatt tcatgcgccg tgacaaaata ttggcggata
    12901 tcctcaggga tttcttccag atcatgaata gtcccatgtt cggcaatacg ctgcattaat
    12961 tccggcgagt agaatcccct ttctttggcc agagcttcga agtagggatg aacttccacc
    13021 agaatgtcat tgtccatgac ctgacggacg taagaaacgg caaaaagcgg ttccacaccg
    13081 gaagaagcat tagcgataat cgatatggtg ccggtgggtg cgatggtggt cacggtggca
    13141 ttgcgcaggg gcggctgtcc ttttttatca taaatggaac ctttgaaatt gggaaatgga
    13201 ccgcgcttcc tggccagttg ctgcgatgcg ccgtgtccct gctcgttaat aaacttcatt
    13261 actttttcag ccagttccat ggcttcatcc gtattgtagg gaattcccag tttaatcagc
    13321 atatctgccc atcccatcac gcccaggcca attttacggt tggccatggt catctgggcg
    13381 atctgcggca gaggatattt attcacttcg acgacgttat ccagaaaatg cacggcaagg
    13441 taaacgattt ctttgagtct tgcccagtcg atcttgccct ctttcaccat tttggccaga
    13501 ttgatggaac ccagattgca ggattcatag ggcagaagcg gctgctcgcc gcagggattg
    13561 gttgattcga tttcgcccgc ctgcggggtt ggattgtcgc ggttgagccg gtcgagaaag
    13621 acaatgcccg gctcgccgtt ttcccaggct tgattgataa tcctgttgaa aactttgcgc
    13681 gcattgaggt gtccggtgac ctgcttatcc cgcggattga tcaggtcata attttcgtct
    13741 ctttcaacgg ccgtcataaa ggcttcggtc agaccgacgg aaatgttgaa attgttcaat
    13801 tgttttttgt cggccttgca catgatgaaa tccatgatat ccggatgatc cacccgcaga
    13861 atgcccatat tggcgccgcg ccgggtgccg ccctgtttga tggtttccgt cgctacgtca
    13921 aaaacacgca taaatgaaat cgggccgctg gaaataccgg ttgtcgttaa aacgacatca
    13981 ttggcgggcc gcaggcgtga aaaagcaaat ccggtgccgc cgcctgattt atgaatcagt
    14041 gcggtgaact tcacggcgtc gaaaatttca tccatggaat cgcccaccgg cagaacaaag
    14101 caggcggaca attgtcccag ttcgcgtccg gcattcatta aggtcggcga attgggcaga
    14161 aattccagag aggccatcat ggcgtaaaat tcttcggcgc gtttacgcgt atccgccttc
    14221 ttgttaaatt tcttgtcagc ggcggctatc gtgtccgcca cgcgataaaa catttccgag
    14281 ggcgtctcaa gaatttttcc cgatttgtcg cgtttaagat atcgtcgttc cagaacggtt
    14341 aaagcattct gcgtcaattc cggagtaaag ggggcgagat gtttctgttc cataaagcga
    14401 ttcctcataa atatcgaaag agataaagaa aaagatacta tatattgtat gaatttgatt
    14461 tattaaccac aatatataga taaagtcaag agacggaaga aaattttaaa tgaagaaatt
    14521 caaggagttg agaataaaaa taaataacaa gcaaaaggct ctttaaccgg ccatccgcac
    14581 gttcattaca ttataaatgt tcatccgtcc ggtgaaggct tatggagatc aagtctttgc
    14641 tttgttcagg tttacgattt ccgatgtcct gataatttgt cagacggtct gatcgggcaa
    14701 ttttcaaggc gcatgcgcgt ccataaattt tctgccgtga tctctgccgc tttgataggc
    14761 ggcgcgaaga cgtttgatat cggtcgtcat cctgccgaca tcgatgtccg ggggcggcgc
    14821 tatttcaaaa acgcgcactc ccgccggtgg atgtctgatt aaacgcaccg attccatgta
    14881 attcccaggt ctttttttca tcgcttgcgc aaacagcgga taatccctga aaataatagg
    14941 atagcacatg gaaaacatac tctttttctt gatgtattgc gacggcctgg ttctgataac
    15001 cgttatctgg gttgccctgc gccggatggc ttcttgcacc ggcagagaat ccgtaacgcc
    15061 gccgtccgtc attttttcgg aatggacctc cagaaaattt ctataaaata tcggcagcga
    15121 gctggacact ttcagataat gttccagcgt gtcctcatcc ggttcgagat acgctgattt
    15181 tcccgtcgcg acagatgtta ccgcaatgat gtattctttc tctcgttctt ttaatcgccc
    15241 gaatagtttc ttcaggtcca gacgatattc gctgatggta acgtcccaca gccagtccag
    15301 atccatcagg tgtccgccgc gcagaaatct tcccagattg atgaaccggc cgtctatgga
    15361 atagcgtttg atgatgtcat aatttctgtc gttctggccg gccagatgcg aggcaagatt
    15421 gcaggcgccg gcggaaacgc ccagatacat atcgaacgga tcgaatcccc cgctgccgaa
    15481 ggcgttgaga actcccgcgg caaaaacgcc tcgcatgccg ccgccttcga caaccagcgc
    15541 tttcttgcct tttttaagat tttccatcgt tgtgcttgtc actcgtctta attccggtca
    15601 ccggtgcgta ttgatctatc tggatttttt ctctttctgg attaatgcct tcgcttcttc
    15661 aacggcttgt tcaactgcgc tgatgacatc gggtttgata tcaaaataga aacccgtatc
    15721 cctgcagagt tttttgatct tcttgatttc agccatcagc ggcgccggta tgctgacccg
    15781 caacaatacg ttttttgatt tttcctttag ctctttcctt ataatagcca tcatgcctcc
    15841 ctgaacccat gttctgaatt tttattattg ctataatata atactcatat cttgtcaata
    15901 taaatagcca tattttatag ttataattgt tgctatttat tttgcttttt ttattgacaa
    15961 ttataattgc tgtgtgtata ttacatttgc cggtcggaga aaaggatttg catcatcgag
    16021 ctcggcacga tgatgccggc ctgcggttaa tgattttaaa aaggaggtct tatgaaagag
    16081 aagcacgcta tcgtcaaatt ggttttcggt tgcattgatg acgtggtttc aacaactgaa
    16141 agcattcaca aaagcattgc cgagaacgta aaaggtgttt cccgcagcgg ttttgcggaa
    16201 ggcggaaaac ggaaacacgt atatgacacc attaaggccg ttaacgggaa agtcgaggaa
    16261 ctggtctcag gattaaagaa ataaggattt tctcggacac ggatcgcagg cacgactttg
    16321 atgcatgtct gacaacagtc gcaaaaaaat aggagaggag atttgtatga caagcagaag
    16381 agcaaaaatc ggattagggt atcatttagc agcgtttgct gcggtcaatg ctgtattggt
    16441 ctggatcaat cttgatacgt cgcctgaata tttctgggcc aagtggcctc ttgctgggtg
    16501 ggccgtagcc ctttcatatc acgcgttcag tgtcttttcg tctttaataa aggctcacaa
    16561 gggattctat tatcatcttt tttcatttct gatcatcaat gcttttttga ttttcattaa
    16621 tttcgactta tacccccaat atctctggtt taaatttcca ttaatagtgt ggacgattat
    16681 gattgttttt cacgggtgga gagttttttc agaacgccaa aaagctaaag ccgtcgcagc
    16741 ctaggaaact attttttctt cttatgcttt attacttgaa acccggcgcg atttcgttta
    16801 aagaattttg cattataatt ctatcctcaa aggagtcgcc atgatcaaga tgaaagaaat
    16861 aaaggcaaaa aatatgaacg taaataaatt tatcgacgat aaagtacggg acatccggca
    16921 ggccgtgggc gatggattcg ccatcaacgc cctgtcggga ggcgtcgatt ccgcggtcgt
    16981 gacggccctg ggacataaag cgctgggtaa acgcctgaaa acgtatatca ttgacaacgg
    17041 cattatgcgg gaaaatgagc cggccaaagt ggcggcggcc tttaaaaagc ttgggattca
    17101 ggtcgacgtt ttcgacgcgt ccagagcgtt tttctcggct ctcaaggggc tgaccgatcc
    17161 ggaagaaaaa agagaagccc ttacgcaaac cttttataaa aacgttttcg gcaagctggt
    17221 cgtcaagagc aaagccagat acctgttgca ggggaccatt ctgacggacg tggatgaaac
    17281 cgtagcgggc atcaaaagac agcacaacgt tttcgagcaa ttgggtatcg atccgcaaaa
    17341 agctttcggc tacaaaattc tggaaccgct cattgaactg cgcaaggacg gcgtccggaa
    17401 agtcggccga gggctgggtc tgccggaaga catgtttgca agaatcccct tccccggacc
    17461 ggcgctggca gccaggatta tcggcgaagt cacaccggcc agaatcgctc tggtcaaaaa
    17521 agcaaccgcg atcaccgaag cggcgctgaa agatgtcaag gcgtttcagt atttggccat
    17581 tttgcatgaa gatcgcgtca ccggcatgcg caacaacaaa cgcgtattcg gcaatcagat
    17641 tgaaatccgg tgctggaaca gccttgacgc acgcaccgca cggccgacca gactgtccta
    17701 tgctattctg gaaggcatcg ccgcgaaaat cacaaacgag atcccggacg tggtcagcgt
    17761 cacctacaac atcacgtcca aaccgacatc aaccattgaa gcgatttagc agggaaaacc
    17821 gaaaatttat ttaaaaaaat taacgccctg tcatcgggat ttttttcatc gcgggtcggg
    17881 atgcatcatc cagataacac gtccaaccgg ccgcggcggg agaccaggca aggtcatcgc
    17941 aaaactattt tcaacaggtc ttgccgatgt ttttttattc tttcaggtgc agatgcatga
    18001 tccggcggat ctcgatgata gcgtccggca tttcctgcac gctatgtccg gaagcaatca
    18061 ccttttccga gacggcaccc tcaaggtgcg agctggaata aggcaccaca ctgtcactgg
    18121 attcggctaa aggcttcttc ggagtgttat taccgataat ggaatggtaa cgcacccggg
    18181 atgaaaccgg caacgtggag gtcagctgaa tgaaggggtc gcggttactg agattgtcga
    18241 tgctgttgaa tgaccgggtc agcgagacgg gattggcgga accgggatcg accagcagtt
    18301 gtgccacttc cctgaacctg cccagcacag agaaaggcag tttgatgaaa ttggctatcc
    18361 agcggacaag cgctatttcc gcgacaggcg tccccttgtg cggcgcggcg acaaagatcg
    18421 cccggctgat ctgaggcagc ggttcaaaac tcatataggg tcccagctct ctccggacac
    18481 gctcgagccg tctcccttca agaggatatt ttttaagcaa tgcaccccac aaccggtctc
    18541 cagaggaaga aaccatcaac cgggatagaa tcccgcccat actgtgtcca atcagtaaaa
    18601 ttttctgaga cgcccgcgct tttccttcgg gatcaaaatg tttcagggtc tgtgtgatgg
    18661 ccttgctgat ttcataatgg ttaaacgcaa tgggcagatt ggtcggataa tacacctgcc
    18721 agatctgaaa atttttcctc agagtctcgt cgcccaacac ctcgttggcc agattgatcc
    18781 acgcttccgg gctgctggcc agaccgtgca gcatgataac gatgtggcgt tggggattgt
    18841 atggctgcat gagataaacg cgcggcatct ccagcacatc gcctttcccg ataagcgtca
    18901 gaagagactg agcggcaaag ccggatcggg caagccacag tccgtaaccg gacgtgaaat
    18961 tggccgccag cggcacctcc ttgccgacca gcttgaacga ctcgcggctg taaggatcgt
    19021 accccgtgat gaccacctcg ttggttttca acacgtccat ccaggtgttc cccggaaaac
    19081 tcatcacagc agtaaccgga ggaaagggcg tttcactgaa gggcatttct gcgctttgcc
    19141 ggctaacgac gcgagtggag gtcacggcca ccagttccgc ccccaagccg tcccggcgat
    19201 attgattgcg aagaccggca aacgacagtg aagatgcggg aattaaatcc tccggaaaaa
    19261 tgtgctcttc agccaaacgc aggtcctcat ttcgaatggt gatgtgccat tgtcccgatt
    19321 caaaatgcgt atccttctca aaattaaaat cgggcggcag acgtttacga taatattcaa
    19381 ataaggttgt cagagttttt tcaacggcat agttgtaata gtcccgaacc tgtgtctgtc
    19441 gatcctccag cgcccgcata ttgggcgtgc gttttgtcag aaaaagatag gcataggagt
    19501 atctcgcggt ttccaggtaa gcattcaggg tcgcttcgat atgtccgctt tcgatgaatt
    19561 gcttttcgag ctccatggcg tctttcagcc agagttcagc cagcgccgat aaacgattct
    19621 cactgtgtaa cccgatggtt tccatcaacg ccttgcggca tccggaagcg tccttgtcac
    19681 atactttctt atcgataccg accacctgca aagaggcgcc cgtgtaggtg ctcaattcac
    19741 cggtcgtcag gatgtcacca cggcgaagcg acatgtagtc gccgctgccg acaaacgaca
    19801 ccttgacacc ggcacaaccg gcaagcgtca tgcagaccag taaaagaaac agaggaatca
    19861 ttcgtttcat gtgagggctc cgcattgaaa agtcgttagg gtttattgtc tgaatatata
    19921 aatgaatttc gtaagggtca agaaaatcct aagggtgaca atcggcaaag aaaaccgtac
    19981 caaacgggca aggacatttt ttgttcattc accgggcgtc ccgccctgaa accacggctg
    20041 atgatccggc acgccttccg gatacagggt ctttttttac cgttgctgct tcagaccgtt
    20101 aatcagccgc gttttcaatt tctctggcga cgctcatgta atggaggccg cggaaagtca
    20161 gttcgatcgc gacgggcatg gaaagcaatc cgaatcgtaa cgatttgatg ataaaaacca
    20221 atccataaat gccgaaaggt ttaaatacaa gcttcataat ttcaaagact atctttgact
    20281 tattcggatg actggtcatt ttggacatat ataatttttt taaccacggt tccgcgtact
    20341 tcagttttga catcctcata tccggaaatt ttttcaaaag cgttgacgcg cgggcgaaat
    20401 aattacgggg cgagtaggtt tctttgatga ctcttctgta gccttcaata agctgcttca
    20461 ccggcatttt aggaatgaag ttgaggtcgg tatcaacatt atttccgcta tgattaacac
    20521 caaggacaag ccttccttcc cgctccaacc gattgtacaa atcggtatgc ggcatggcgg
    20581 caagaagacc caccatgctc tggacaatgc tgttcttccg gataaattcc aactggctgt
    20641 cgaatatgtc gtccgcatcg gtatcgaatc ccaggataaa tcctcccata acttctatgc
    20701 ctgctttctg cacctttgct atggcagcat tcatgtcgca ggtcacgttt tgattcttat
    20761 tagtggcacg gagcgtcttc tcatcgggag actcaatccc gataaaaacg gaggtgaacc
    20821 ccgaccttct catcagcccc agcagttcat catccttcgc aaggtcgata ctggcttcgg
    20881 taaacagcag aaagggatat ttacgctcct cctgccacgc agccagcaat ctgagcaatt
    20941 ctttaacttt ttttctattg gcgatgaagt tgtcatccac aacaaaaaga cgcccgcgat
    21001 atccggttga ataaagcgcc tcaacttctc ccataaattg ctctggtgtc ttggttctgg
    21061 ggatgtggcc gaacatctga atgatatcac aaaattcgca attgaaagga cagcctctgg
    21121 aaaactgaag ggccatggag acataatccc tcggacgtat taaatcaaag cggggaatcg
    21181 gggtctgtgt gagggaaggt ttttcattgt tatcgtaaac tctcttcgct ttgccctgta
    21241 cgtaatcatc aaggaattgc ggaagattgt tttccgcttc atataaaatc aggtgatcga
    21301 tcttgcttat ttcgaggtca ttgcttccct tcgtgccgta aaccgattgg acaaaaggac
    21361 ctccggcaac aacgatcttg cctttttttt ggcatatatg gaccatttcc ataaaagact
    21421 ctctgttgac gatcataccg gttagaaaaa ccatgtcact tttatccaca tcccgcatgt
    21481 ccagatcttc aatattgaga tccactaatt taacgttgaa gttttgggga agcatggcag
    21541 ctaccgtcag caatcccagt ggcggcatgg tggctttgaa tccaagagcc ttaatggtat
    21601 ggtcatggct ccaaaaagtt tgcggcgtct tcggttgtat catcaacaca ttgatatttt
    21661 tcataatttt tcctatggcg ggcaataagc ataaaaaaac attatccccg tcggtttttt
    21721 gaaaagatat gcgcagaaaa tttaaaaagt ataggatgga gaaggatcgt gcattaactt
    21781 tccaaacggt tctttttcat aaattaccgc atattaaaag ggcacgtcgt ccaacggcac
    21841 gctgccgtca ttcacattag cggcatcagc atcaaaagaa gtctctccgg atttggtttg
    21901 tcctttggaa tcgagcatcc tcatatcact ggcaataatt tctgtcgtat aacgtttgac
    21961 gccttccttg tcttcccagg aacgcgtttg aatgcgacct tccacataaa cgagcttccc
    22021 tttcaccaga taattaccgc agatttcagc cagttttccc catgttacaa ttttatgcca
    22081 ttctgttttt tgaatttttt cgccgttttt gtttttatct tttctctgct cgtcggtggc
    22141 caaattgaag ttggtaacca tcgtcccatc aggcgtgtag cgaacctccg gatccttgcc
    22201 caatcttcca atcaagataa ccttatttac catcttcctt cctccttgtg atctgccctt
    22261 acgggggata cataacatta cagggagatt gtggcaaacg aattttataa gtatcgattc
    22321 ttccgcatta atttacggca atcttaaaaa agatatctgt attttcatta aaatatgtta
    22381 atattcataa aaatatttgc gggattggca atgatttctt acatgaaaaa tcatgaaaat
    22441 tacgtaaaag gcattctgga gcaaaatccc gatgccgaaa aattaaagga gcttctcgcc
    22501 taccacgaca aacagatcaa atggatacag catgaacgcc tggtccattt aattgtgatg
    22561 ctcttcgttt gccttttcac aatgatgatt ttcggattcg ccgttatcag accttccctt
    22621 tccgttattc tactttttgt tatcctgctg attctatccg ctgcctatct tctgcactat
    22681 ttccggctcg aaaatggcgt gcaaaaatgg tatgtgatcg ccggtgaaat cagacggcag
    22741 ttttaaatga cggctatccc tttataatcg taaggagaaa acaatgactc aaaataaaat
    22801 cgtggcacat taccaggacg gcaatttgtt taaaggcatt accaacgatt tttcccccaa
    22861 taaggaactt tttcatctgt ttgagagagc ggctcaggaa gacaacagac cgttgacatt
    22921 gaagatggac ggtctaaaag gtttattcta tgttaaagat tatgacggca atccaagtta
    22981 tcacgagcaa aaagagttcg acccttcccg acccttggcg ggacgaaaaa taaaggtcat
    23041 ttttaaagac ggggaattaa tggtcggcac aaccaacggc tatcagccca accgtcccgg
    23101 gttctttctc gttccggccg atgcaaactc caataacgaa cgttgttttg ttgtctcttc
    23161 cgccgcaacg gaaattgcgt ttatctgatt gacggtgatt tccgtctgct cttaagacag
    23221 gctcttcagt ccacgacctt tccggtaacg caggaatttg ccgaaatgca ggtcgagctt
    23281 gctggtatga ttaataagcc agtcaacgat cagcacctga atgcggaaaa gaataaaaat
    23341 cctgttagtt tcaaaatttc tttttatttc atttctcagt ccgtcgaagt acctcgaaaa
    23401 gcgccggtgt tgatcgactt gcatctgcaa aaagggatag tttttttgcg ccattaaacg
    23461 ttcttcggtt cggaaatgaa cctgaatgta tttatccata aactcaagga cattgcggag
    23521 ctgcgaataa tttttgcttg tcttcaccgc ttccacaaac tcccgggtgt gaataaacag
    23581 gtcacggtgc tgctcatcga tgagggagtg atctatcgcc atcgatttat tccatgaaat
    23641 agccaggtca aattccccgc tgctttcatg aatgcctgtt ttcaaaaaac ggaggcaacg
    23701 ttcatgataa acctttgcca cagtatcccc gggcacccgt cgcagaagct tttccagcag
    23761 cttcgtcgcc ttgggaacat ctttgaaatg ataaaaggcc agcgcttctt caaaatcggc
    23821 ttttgttttt tgcttagccg tgcgtaaaga tttcggatcg gcgtcaaaga cttcataaac
    23881 cgactggcat tgtgccttcc ccttcacccg aacgcggtcg acaaaacgtg tattgtgttc
    23941 agatgcgccg aacaaactgt aataggtgtg ctcgctgatc agaaaaggga cattgtaatc
    24001 tttcgtcatg gactcgatgc gggacgccag attgactgcg tcgctgatga cggttgtttc
    24061 cataaaatgc tcgccgccga caatgccgag catcatcagg ccggtattca atccgatgcc
    24121 gatcttgata cgggaagaac ctgttttttt aatcgagcgg ttaaaggcga ccagttgtct
    24181 gagcataccg atcgcacatt tcaccgcgtc gtcggcatcg gcggggaaaa gcgccatgat
    24241 cgcgtctccg atgtatttgt tgatgatacc gccgtgacgc gcgatcaccg gattcatgca
    24301 cttcatgtag gcattaataa acgtgaaatt ttcccggggg ggcatttctt cggaaagaga
    24361 ggtaaaccct ctgatatccg caaaaaggat ggtcattgtt ttttcaatgt gatcgccgag
    24421 ggaaacgctg gtgatgtcgt ctttccccat cagggcaatg atttcccgcg acacaaagcg
    24481 gccgtgggcg ttgctgcccg gaacggtttc atcccgcggt tccggttttg aaagctttgt
    24541 agcgagcgct gtttcctttg ttgtcacacc agacattcag actttcccgg aaacggacat
    24601 cagagttcta tccatcattt ctaatgagca aacatatttt taaaaaatat ttctaacgcg
    24661 ctcaatatcg gcttgggcct cggcaaccag tctttccata agctccttgc aggtgggaat
    24721 atccttgacc agtccaaccg attgtcccat catcattgga gcgctgtcca catctccaaa
    24781 ctcccaggcc tttttgacgc gctctccggc aatcagcgga atcagttttt ccagtccgcc
    24841 gcccgtcgat tccacggcca gaacatcctc caccagtttg tttctgatgg cccggccctg
    24901 taggccgatc gatttgcaga tcagcgctgt ttcgaattcc tgacgctgga taatttcctg
    24961 cttgatattg tcgtgaactt cgcattcttt ggtggcaaca aaacgggtcg ccatcatcac
    25021 gccctgggcg ccgagagcca gtgccgcggc aagggttcgt ccgtcagcga caccgccgac
    25081 cgttaccacc ggaattttca cggtttcggc aatacgcggc gtcagcacca tggtggtgac
    25141 atcgtcattg agaggatgtc cgccttcttc aataccggcg gcgtaaatgc cgtcgtagcc
    25201 ggtcttctcg gcgtgcacgg catggcgcac cgatccgacc ttgtggaata atttcacacc
    25261 ggcttttttc agaatgtcta taaattcctt gcccaccgcc ttatccaacg gggttccgga
    25321 tatctccaga ccggcgactt tttcttcagc acacactctt aaatacattt tgtgatgttc
    25381 cgctgttaca tggactgacg gcagaatagt aacatttacc ataaaaggtt tatccgtcag
    25441 ttttcgtgtc tgccgaatgg catcacggaa ttcgtcttcc gtggggtaat ttccggccgt
    25501 cagattgccc agcccgccgg cattggatat ggcagcgcac agtacgggcc tgcaaagcca
    25561 catcatggct ccgcagataa tcggatattt gacgctgaac atttcggtaa tcgctgtttt
    25621 cattttttgt acctctttaa atggtcataa cttttgttca atgctaatcc atcatcacca
    25681 gcgaagtaat cttggaagtc ggaggcataa agtcatcatc cagaactttg atgatatcgg
    25741 cgtacagcct ccttttcagt tcgccgaaaa ttgtccgttt tttctggaaa gtcttggcaa
    25801 aagctatcgc ggaggacatt aattcttcct ggttggcgca ggccttcaca ataatgttgt
    25861 gcttctccag ttccggcgcg gtgaaacgat taccggtcaa ctgcatctgc gacaacaagt
    25921 ggatcgggat ggccttttta acaaaagcgt tcatccccca gcggaagggg atgctgatgt
    25981 tgacttcggg aaagcagaag aatcctttgt cggagcgcat gaatcggaaa tcgcaggcgc
    26041 aggcgataat cgaaccattg ccgaaagcat gtccgttgat ggctgcaatg accgggatcg
    26101 gataaagaag caatttttta aaaaccacat ccatttcgtt gatgaaatcc gttaccggct
    26161 gatgttcatt tttctgcatc tgccccatga tccattcaac atcgatgccc tgcgaaaaat
    26221 tctttgggtc attagaagta ataatcagag cggttatggt tttatccgcg attatttcgt
    26281 caagcatcgc attcaatgtc ttggcaaatg tcagattttg tctgttttca ccattggtca
    26341 tggttataat ggcgacggtt tcatctttgg tccagttcat gatagacata tgttttacct
    26401 ctctttttca aaatgtaaaa attacggccg caaaggctat aatattaaac tatttcaaca
    26461 gcataagccc agcccgcaac cggaataacc caatcgcgag tcgttggagt ataagaagtc
    26521 ccccatcgta tgtcaaggat ataatacgcg ttgtccagcc aatttagaaa tgtcctattt
    26581 ttaccaaaat agaaatgtcc tattagaaga aaaaatatag atatcaatat cgttctcata
    26641 aaacagttgg cgttgtcccc caggttttcc acctttgctt ccatggatga tcttttgatg
    26701 gtttaggggg ccggttatat gctctcaggt ctttcttaac agccaccatc tgtttgggtc
    26761 tttcggttat ttctttgtat tttaaagctc tgcccttgct gatgatgaga agagaaccat
    26821 ctcttctctt ttcaatatgg acctttttac tatcgttctc ctctaattga taaagctttc
    26881 tgttgtaggc aatggtgtga tcattacgga tcgtcctttg gtctttgata cagagatatt
    26941 tgtcccagtt aaaatgacga ggaggtctga catgaacatc cgtctcattg gccggacaca
    27001 ctctgaattt ttcattatat tggggcaagt acgtttccag aaacccattg gcttcatctt
    27061 ttgtcttgat gccgtggagc ctcatctcct tgaccagccg atcctgaagg actccaaaga
    27121 gccgctccac ccttcctttc gcctgcggag acaaagcatg aatgacttcc acgccaagct
    27181 ctttaaggac cctctcaaac tgactgagaa actttagatc acttgcttca tcccattccg
    27241 taggtttcct ggaagacttg taggtcgtgt gacgatcaag ataaacgctc atgggaagac
    27301 cgtacttctt gacataaccc ttgaagctgt ccatggccgg caaggtccct tcataatcat
    27361 aaaattttgc atagattgta ttcgtggcat catcgatata gcccattaat accagcttgg
    27421 gacctcgacc ttccaaccag tcatgatggg acccgtccat ttgaaccatt tctccaaaac
    27481 aggatcgcct tggacgccat tgcctgtatg ctgatcgctt gcgatgcttc ttccacaaac
    27541 cggcccctat cagccaattc cgcagtgtct catcgcttaa tcggatacca tctcgctccc
    27601 atagcttctc cgtcgcaaaa gtgggtccaa aatcagaata cttctccttg tatagagcga
    27661 tgacgttctc ttttgtccca gcggataatc cgcggtttga tggccgacca cgggatctat
    27721 ttataatacc cttatcgcct tcctcgcgaa cggccctcac taaccgcctg atctgacgct
    27781 cactgagtct taatatgccg gatacttgct tctgactgat ttgtctgtct atcgccgact
    27841 ggatcaactt cagacgcttt aattctccta aactcatgac caccatgtcc ctttcagcca
    27901 taattacctc cccccttgca ggaaacaatt acggctttat cttaggacat ttctactttg
    27961 gtggaatagg acattatcac tttggcggaa catgatataa tacgcatccg aatggccttg
    28021 aaatttatct aacaaagtat taacttaatg aaaattgtcc tgtgaatttt tcaaaacaaa
    28081 atttgttcat gcccgaggag gtctgaaatg ggattgaaaa ccgctgatca atacgaagaa
    28141 agcctgcgta aaatgaatct gaaagtctac cttttgggtg aacaggtgaa atgccccgcc
    28201 gatcatccaa tcatcaggcc ttccatgaat tccgtaaaaa tgacgtacgc cctggctcaa
    28261 agccccgagc atgaagatct gatgacggcg aaatctcatc tgaccggaga aaagataaac
    28321 cgtttctgcc atctgcatca aagcacggaa gacctgatca aaaaagtaaa aatgcagcgg
    28381 ctgctgggac aaaaaaccgg cgcctgtttc cagcgctgtg tcggcatgga tgccatcaat
    28441 gccgtggaca gcgtcacgtt cgaaatggat aagaaactcg gcacgcaata ccatgatcga
    28501 tttatcaggt ttcttaaaat ggtgcaggaa gaagatcttg ttgtcgacgg cgccatgacc
    28561 gatccgaaag gcgaccgcag tctgccgccc agccgtcaga ccgatcctga tttgtacacc
    28621 catgttgtgg aaaaaagaaa agacggcatt atcgtgcgcg gcgccaaggc ccaccagacc
    28681 ggagccgtca actctcactg gattctggtg atgccgacca tcgccatgac caaagacgac
    28741 gccgattacg cgctttcctt tgtcgcgccc gcagacgcgg acggcatttc ctacatctac
    28801 ggccggcaat cctgcgacac gcgcaaactc gaaggctgtg aaatagatgt cggcaaccgg
    28861 caattcggcg gtcatgaagc cttgatggtt tttgacaatt tattcgttcc ctgggaaaat
    28921 gtcttcatgt gcggggaata tgaattcagc ggactcctgg tggagcgctt tgccggctac
    28981 catcgtcaga gctacggcgg ctgcaaggtc ggcgtgggcg acgtgctgat cggcgcggcg
    29041 gcactggcgg ccgattacaa cggcgcggcc aaagcctctc acgtcaaaga caagctcatt
    29101 gaaatgacac atttgaacga aacccttttc tcctgcggca tcgcctgctc tgcgcagggg
    29161 cacaggaccg cttccggaac gtacctgatc gatctgctgc tggccaatgt ctgcaagcaa
    29221 aacgtgacca gatttcccta cgaaatcgcc cgactcgcgg aagatatcac cggaggattg
    29281 atggtgacca tggcctcgga aaaggatctg cgtcatccgg aaatcggcaa aatagtcgaa
    29341 aaatatttca aaggaatcgc ttccgtgccg actgaagacc gatgccgaat tatgcggctg
    29401 gtggaaaata tcacactggg cactgccgcc gtcggctacc gaacagaatc catgcacggc
    29461 gccggttctc cgcaggctca gcgcatcatg attgcccgcc agggcaatct ggaacacaaa
    29521 aagaaactgg ccaaagaaat agccggcatc aaggattgac cgggcttgcc ttgatgaact
    29581 gcaaaatatc agaaagaaag ttgattggat ttaaatactg cggcgggtgc aatcccgtca
    29641 tcaatagagc acaactcgtt caggacatcc aaagacggct gtcggctgag ttcactcttg
    29701 caacggatca atctccaact caatgggaca tcggtattct cgtttgtggc tgtctgagcg
    29761 cctgcgcgga taagccggat ttcaggaatc ttgcccgcag atggattatc attgccggca
    29821 attcggttga ttacggcgat gcgccggaaa aggatttggc tgaaattgtt ctaaacaaat
    29881 tgaagctata aaatattcta attcggatat ccttgaggat ggttgagtca aaaagactca
    29941 acagaacaat gcgtaaaatc aggccttaaa tctctcaaag ccgttcatct ccttgtgaag
    30001 ccttcatcat acattcgtgg aattatctaa ttcccttaag cattattccg atccttcccg
    30061 tttttatcaa tggatatcat cgtgacatac tatgatattt ctattttatt tcatagccac
    30121 gctctgaaat aaaataattc ttgacagtgc gactatgaat atgtatctta aaatcactct
    30181 cgcgggagga aatgatcatg acagacaaga acagacccaa gacccgaaaa gaccgcatta
    30241 tggatgccgc tctgcgcatc tttgccgaga agggttttca gaacgccacc atcaccgaaa
    30301 tcagcaaagc cgccggtgtt tcggaagcga caatctacga atacttcggc actaaggaag
    30361 accttctctt tgcgattcct gaaaaaataa ccaacgacac atttgaggaa tcttcaaagg
    30421 tcattcccta catcaaaggc gtggagggca aaatcagggc gattttactt ttctatgtca
    30481 agctttatca atccaatccc aactattccg cactggttct cctgcaactg atgtccaaca
    30541 ggcgctttcg acagacaccc gcccatgccg caatacgcac atcggcgcat accctgctgg
    30601 actgcatccg ggaaggaatc gccgacggca cattcaaaaa ggattccaat ccctacctga
    30661 ttcgctccgt gtttatgggt acgatcgagc atttattcat tcactggcac atgcagggca
    30721 tgcccaaaaa aaaaccgggc atcgcggaga tgcttgacgc cctgctcgaa accgtttttg
    30781 acggcatccg cgcgaggaaa gaggattccg gaacaacgct ttacctgaaa ctggacgatg
    30841 ccggcctcct ggaaaaaatt ctgaagaagg gcagagaacg tcatcaaaat aaaaaataag
    30901 ataacatttt tagcaaacaa agaagaggta ctgccatgag aacgagagaa caatacattg
    30961 aaaggctccg gaaaatgaga aggaacctct accatgacgg cgaaaagatc gaccgtgatg
    31021 atgagatcca ggctcccgtg ctcaatgtga tgggactgac tttcgacgcc gttcatgatc
    31081 cggagttaaa agatctgtgc accgcgactt cccatttaac gggagaaacg atcaatcgct
    31141 tttgccatgt ccatcaaagc acagacgatc ttcacaaaaa gcaggacatg acccgggctt
    31201 tatgccggaa ggcagggatg tgtattcaac gatgcatggg gaccgatgcc ctgaatgccg
    31261 tcaacgccgc ttcgtttgag gccgataagg tcaacaacgg caatacacaa taccacaaga
    31321 atttccttaa atggctcgaa cgttttcaga aggaagacct tgtgggaagc tgcgcccaga
    31381 ccgacatgaa gggcgagcgc ctgaaacgac ccgcggaaca gacggacccg gatatgtacg
    31441 tccacgtcgt cgagaagaaa aaagacggca ttgtcgtccg gggctgcaaa ctccacatct
    31501 ccgaggcgtc aatctccgat gaaatcattg tggtgcccac ccgcgccctg ggtcccgatg
    31561 aaaaagatta cgccgtggct tttgccgttc cctccgatta cgaaggggtc aagcaggtcg
    31621 tttccattca caaccagcac aagcgcaaac atatcaaacg cggcatagac atcggttatt
    31681 ccgattccta catcattttc gaggactgtt ttgttccctg ggagcgggtc ttcctctgcg
    31741 gcgaaaacga tcacggcgga atgtgcgccc tgctgttcgc gctgttccac cgccacagct
    31801 attcgggatg caagcccgcc attggggatg tttttctcgg cctggcagct ttggcggcgg
    31861 aatacaacgg catcgagaaa acgcctcacg tgaaagagat gctggcggag atcatcaaag
    31921 tgtccgagct gggttatgcc gcagggtata cggcttccag catgggcaag cccgaagtca
    31981 ttatccccgg cctgggtatg tgcccttacg gaccaggcag ctacatcccc aattccattt
    32041 actgcaacgt gggccggtgc ctaacgggag aagcggtttt ccatgaacag gaactgatct
    32101 gccggatcgc cggcggcctg ccgtccacct tcccctatga aggcgacctc acgcatcccg
    32161 acctgaagaa agttctggaa aagtatctca accggaatcc caaggtccct gtggaagacc
    32221 agatcaagtt ttggatgacg ctgggcgact ataccatcgg caccatggcc ggtgtcatga
    32281 actacgggaa ctatcacggc ggcggatcgc ccatcatgga gcagattgcc attacgtccc
    32341 agtatgatat caaggacaga aagaaactca ttaaacgtct ggcgggcatc ggcggttgag
    32401 ccagagaagt ttgataattt cactggaaac agaaacaata ttgtaataga catcatttgc
    32461 cccgtcgcca acaacagagg aggcgttaga tttctcaagg agaataaaat atgctcgacg
    32521 tatcccaatt ttcattaaaa gataaagtgg cggtgattac cggcggaggc cgcggcatcg
    32581 gtcaggctat cgcgttcgct tttgccaggg ccggagccaa ggtggttgtg accagccgca
    32641 aggcgccgga tctggaagcg acagccaacg aaatcaaagc tttcggcggg gacgcttacc
    32701 cgctgccggc tcatctgggc aaatcggaag aaataaaaaa gatgattgac gccgtcatgg
    32761 ccaaatacag ccggattgat attctggtca acaacgccgg cgccagtccc gcgaaaggtt
    32821 ccgtcctgga gtgcgacgaa cgcctctggg acaagctgat ggatatcaac ctcaaaggcg
    32881 cgtattttat cagccaggca gcggccaata tcatggtcaa gcagggcggc ggcaagatca
    32941 tcaatatcgc ctccatcgac ggtcacaatc cggaaccggg tctgagcatc tacgctatct
    33001 ccaaagccgg cattcgaatg ctgaccaagg cttttgccag cgaacttgtc cggtacaaca
    33061 ttcaggtcaa caccatcgct ccgggaccca tcgccaccaa gatgttgaaa tccaactggg
    33121 aatttcttcc ccctgaagac gctcaaaaag tgaaagaact ggtggaaaaa gcgctgccct
    33181 ccggccgcat cggcgatccc gatgaaatag ccggcgccgc gctttacctg gcatccgatg
    33241 cctccagcta cactaccgga gcggaaattg taattgacgg cggtgttctg ctcggcatgc
    33301 acccgtcctg acaggagaaa gctcgatgat cagtcgaacg gcagcggttc tggtaatcat
    33361 tgatattcag ggcaatctgg ctcaggccat gtttggcaag gaaaatattt ttgccaatgc
    33421 cgtcaagttg attaaagcat tcaagacctt gaatctcccg ataatcgtca cggagcaaat
    33481 tccggggaaa ctgggtaaaa cacttccgca aatagcggac gaaattgaag acttcaatcc
    33541 gatcgcaaag gaatccttca gctgctgggg tgaaaaaaat tttaaagaac gactggaaag
    33601 cttaagccgc aggcacgtcg tgctgctggg cattgaaacg catgtttgcg tttatcagac
    33661 ggcgctggat ctcctgtccg aaggatacaa cgtgcatctg gtggccgatg ccgtttcatc
    33721 gcgcacgccg gagaaccgtc agatcgggat taaagcgatg aaaagcgccg gagctcatat
    33781 cgccagcacg gagatggtct tatttgaact gctgcgcacc gccgccgatc cgaaggccaa
    33841 agaattcttc aagatcgtaa agtaagaaga aattcattat tcagctcaca aaccttcttt
    33901 gaatttactg tcttctgaaa tattattttc tgattccgcc gaaaatcacc ttcagccctt
    33961 tttatgattc cgctgtggaa catcacaatt ctatctgggt gccgagttca accacccgat
    34021 tggcgggaat tccgaaataa gacgtcgggt tccccgcatt tctggacata aaggcaaata
    34081 atgatttgcg ccatttcatc attttcgatt ttcctcccgt aagcagagtc tccttgccca
    34141 ggaaaaatgt cgtcgtcatc gggtctgtaa caagcccata ctgggcaagt attttcatca
    34201 actgcggcac attgggtttc tgcatgaatc cgtaaaatgc ctccacccgg taaaacccct
    34261 gacccagatc ggctagctta atgcgttcac cggcaggaac ggccggtgta tctttggtga
    34321 taatggaaag aagcactact gtttcatgca ggatgtgatt atgttttaca ttatgaagca
    34381 gcgtcggagg cgttcccacg ggagacagcg tcataaaaac agcggttccc ggcactctgg
    34441 gcatatggcg cttcggcaat tcagaaagaa aactttccag cgggaacctt gctccgacca
    34501 aattacggta aagttcattc cgtccttttt tccaggtggt catcgctacc gcaatgattg
    34561 ctgcgatcaa gagaggcaac caccctccat cagcgagctt gaaaaagttt cccgtgaaaa
    34621 aagcgatatc gactaaaaga aacagtccga cgaggggaat gactttccgc aggggccatc
    34681 ctctgtgatg ggtcagcacc agaaaataaa gaattgaggt gatgatcata gtgcccgtca
    34741 cagcgatgcc gtatgcgccg gccagagcgc cggattcccg gaaccccgtc accaccgcaa
    34801 tacacgccat catcagggca tagttgacag aggggatata tacctgtccc tgcatatcgc
    34861 tggaagtgtg aataatccgc acccggggac ataatcccag ttgcgcggcc tgttgcgtaa
    34921 gtgaaaacgc gccggatatt aacgcctggg aagcgatgac cgtggcggcc gtggaaagtc
    34981 cgatcatcgg gtagagcaga atttccggca ctattttata aaaagggttg acattcattt
    35041 cgggatgcgc gagtagcaga gctccctgcc cgaaatagtt gcaaagcaat gaaggaaaaa
    35101 caagtgaaaa ccacgacagg cgaatggcgt ttcggccaaa atgccccaga tcagcgtaaa
    35161 gcgcctcgca accggtaata caaagcacca ccgatcccag caccacaacg ccgtgcattc
    35221 cgttgttcag aaaaaactcc acggcatata gcggattcaa cgcccgaagg acggaaggtt
    35281 cgcgtacgat ctgcatgatg ccgaaaatac caatggacag aaaccataaa accatgatcg
    35341 gagcaaacac ctttccaata ttcgacgtcc ctttgcgttg taatacaaaa agcagaatga
    35401 gcacgacaca ggtcaacggc aatataaaag gcgcagcagc atgggtagca acctccaatc
    35461 cttcaattgc ggaaagaacc gatatggccg gcgtaatgac gccatccccg tacaaaagcc
    35521 cggcgcccag aatcccggca agcacaagaa cggactgaac aaggggagaa aaacttttct
    35581 tcgacacatt caaaagactg accagcgcaa aaatgccgcc ctcgccgcga ttatcggcac
    35641 gaagaaggaa aatgacatac ttgatgctga cgattacgaa catcgaccag aagataagcg
    35701 acaaaacgcc cagaatattc gttatgtcgg gagcaagggc gtgctgtccg taaaaacagg
    35761 ctttgatggc gtaaagaggg cttgtgccga tatcgccgaa aacaacaccg atggatgcga
    35821 aggcaagaaa aatgattttt ttattaaacc cgccggaagg gggctctccc ggtgattccg
    35881 tggccgattc attctcggaa tgtgctgaaa acataaaaaa cctccttgaa tttcccgggg
    35941 agaataatca tcccggacga atccggtcgg ttctcctcat cgaagcctgc gaagttagct
    36001 gaagggctcg ggccgaaaag gttaccctac gcgcaagcga tacgcccctg aaaattgggt
    36061 cctccgctcc tgaaaatcag gactaggcat atgtttggcg ctaattaaat catcttttca
    36121 ggcgtgtcaa tacgtttgtt ggagttcatt tccgaatggt tctattacat tttgacaatt
    36181 aaagactccg gccttgactt ttgcaattcg gtgttgtaaa taaaatgcga ccttttaagg
    36241 gaggcagttt atggtcccgg tcgccaccaa cccgtatgta aatagcgttt ctttcattga
    36301 tattcttgtg cagggcaacc ctgctttttt tttatttatc caaaaaaaat taaaaaacat
    36361 tttaattccc gtgacaacgg ggaagaagga ggatttttat tatggcacaa gctcaggtat
    36421 ttgaaaataa acagccggtt ctgggaatca acagtcttgg gcgaatcggg aaactcactc
    36481 tgtggcatca catcggaaga aagtatttta aggaaatcat cgtcaatcag ggacgggaag
    36541 taggcacggg ccttgattcc gtggcccaag cgatcgagaa agattccacc tacgggttga
    36601 tgcacaagtt tttatacggc gtcaaagcag aacgcatcat tcagattgtc gatgaaaaag
    36661 aggggaaact gctgatcgac ggcattcccg tgaccatcct gcgcgaggca agaaatcccg
    36721 cagatgttcc ctggaagaag tacggcgtcg atgtcgttgt ggactgcacg gggaaattca
    36781 aggatccgac ggtcgcggca gatcacaaga gcggttccat tcgtggacat ttaaacggcg
    36841 gcgcgaaggt cgtgatcaat tcatcgcctt tcaaaatcaa gaacaaggcg ctggcgcttc
    36901 cggatgatgc ggtgacaatg atctacggca tcaatcatac gtcgttcaat cccaagaaac
    36961 atctgatcgt ttccgccgca tcctgcacga cgacgggatt ggctcacatg gtcaagccgt
    37021 tgctggagaa tgaagagacc agcaatattc tgacggcctc catgtccacg gttcacgccg
    37081 ttaccaatac ccagagcgtg ctggacagcg ttcccaaggc cggcgaaaaa gatttgagaa
    37141 aaaaccggag tattctggac agcatcattc tgacatccac caatgccgcc aaagcgctgg
    37201 cggaagtcat tccgcaggtc cagaatatcg gcttcatggc cgattccatc aggataccgg
    37261 ccagcacgat gtcgctgatt gtcctgaatg caacattcca ggcccgcagc cagggagcgg
    37321 ctacggccat caatacaaaa atgctcaacg agctatacgg caaagcggcc gccaacaacc
    37381 ctgaaaaact gatccggttt acgatggaac agaatgtgtc ggctgatctt ttcggtatcg
    37441 acgcggccgt catcattgaa ggccagttta atcacacgcg cacggccttt attaatctgg
    37501 atctggccca tattcccaat atgccggcag aggttgccaa ttccttcccc gacaaggtca
    37561 tgaaagttcc ggttgtgcat gccaagatat tcggctggta tgataatgaa tacggcagct
    37621 acacgaaccg aatgggggat ctgacggttc acatccacaa aaatatttta taagaaagtt
    37681 ttagccgttt ttttgaggta tctgcattca ccgaccgctc gcttccgcgg tcggtgaatt
    37741 tttttctgct attattcggt ttcgctttcg tgccggccaa ggtaaactgt cccgctccaa
    37801 ccgtcgatac tgataaaatc acctgctctg atggtctgat tttctatggc ggcatattca
    37861 tctgtttcgt aaactttcag tttactgaat ccgacgacac cgaccttgtt gagttgcgga
    37921 atcgtgaccg cggcatgcga tgtgctgccg cctttcgcgg tcagcaggcc ttccacttgc
    37981 agcaaaacac ccacgtcatc cggaaccgta tcgggacgca ccagaatcag aggcgtttgc
    38041 ggttcttcac tgcggaatcg tttgatatcc gcttccgaat aaaccgcccg accggccaga
    38101 gctccgccgt tgacgccgat gcccgctccg atcatggatg actgcaacgc cggagaatct
    38161 ttaaaacgtc tccagttctg cgtggtaatc tgattcatgt cgcgggtttg cagaatgtac
    38221 aatttatcgc gcgtcggccc ttcaaaggta aattcgatct cctggtgatt gaaccctttt
    38281 tcgtaaatga ggatctccga aattctcagc agttccgcat aaatttcagg gaatttatct
    38341 tccagggaaa tcttggaatc ccggcattcc gcgatgcgct gcttttccga aacgggatac
    38401 gtttccacca gtcccgaaac gatatcgtcc ccctgaaccc cgaaaatgaa atctccgtag
    38461 agggtcacat cggaagacgt gcttttcggt tctcgggtaa aaataacgcc ggatccggaa
    38521 ttatcgttga gattaccgaa aaccattttc tgcacgatga ccgccgttcc ccatttatcg
    38581 gaaatatgca tttgccggcg gtaaatttcc gcctgttccg aataccagga atcgaaaacc
    38641 tgcaaaatgg cgtcgcgcag ctgatcgagc ggcttctcct taaattcgat gccgttgtcg
    38701 gtcagccctt ttttatagga cagggcgatc tgtttcatct gctcgggtga aaactggatt
    38761 tttctttcca cgccgtactt ctgtttgaat tcgtcaatga tcgcgtcgaa aaaattacga
    38821 ttcagccctt taaacatgcc ccacatctgt aaaaaccggc gataggaatc ccaggcggcc
    38881 catttaaaac catctttctc gctgagattt tccgcaatcg tctcgttgat gcccacgttc
    38941 aaaaaagatt tcatcatgcc gggcagggaa accgtggcgc cgcttctgat cgagagcagc
    39001 agaggattgc ggcggtcgcc aagcttccgt cccgtaatct tctccagttt ttttatttct
    39061 ttgttgaccc gaacagccag atcgttaaaa atatatttat acccgtaaac cgcttcatag
    39121 cccctgaaca cttccgtggt gatgatgaaa ccgggcggaa cattaaaccc gaatgagaca
    39181 agctctttga ggaaatatcc cttgttcccg agcagaatct gattatctac ttttttgttc
    39241 tttttgtaga aagaagacac ggccagttcg ggattgtacg ccataacgac cttcagaatt
    39301 tgtttgttgt ccctgaactt ttcggattcc gcattcagtg ttttaataac tgaattaacg
    39361 aaattatcca gcacctgaat gccgaaagcg gatgcgatga tgccccggat gaaattttcc
    39421 gactgtgcat agatcagttt ctcatgttgc ttttgtccgg attttctctc ggaatcgatt
    39481 tcccgccggt atacctggcc gatcacaaca ggcaggcagg tggtgtggat atcaatgtaa
    39541 tagttgcgaa taatgtcctg aatatccttg gaaatgaaac gaaaaatatc cacgtactgc
    39601 tccagggaaa actgcttgat ggacaaagcg ttggtgacgt atttgatttt ggctgccaga
    39661 ccttccgtgg cgatgccttc aatttccagc gcattcatgt aaagccaaag gtatgtgtga
    39721 atttttctga tcgtgtgctt ggtaatgaac ttgagattga aggatttgct cagttcctcg
    39781 aacagcatgg tggccagtgt ttccagccgc agagaaaggc cgaccgcttc gaattttctt
    39841 tcgtaatatg ttccgtacat ggagggaatg ccggcggcaa tatgcctctt gtaatagatg
    39901 ttctcataag aaggcgtctt tttgggcgag agaattcttt ccttgagaat ggatagaaac
    39961 ttcagaataa tcgtaatgct gcgatgatag ttcttcgtcg tcaaggagcg tttcaacgac
    40021 gagatatccg ctttgggaaa agcgttgacc gcttccagat ccttgagcag atcgataaac
    40081 tgtgggtaat attttttgta gatcagctgg tagatccgga tcatgatttt gacccgttcc
    40141 tgatccctcg cattgatgcc tttgacgttc gccagttcct tacagacttt ttcgttatcc
    40201 cagtccagaa atttcagggg gctttggact tttgtgaaga aatatttaaa ggctttgtgc
    40261 attccggtga aatattctcc gggattcatc acctgttcgt aaacttcaac gggaagatga
    40321 gagcgaagat aactcatatc tccggtattc cagtactgaa aaatatcctc ggtaaattta
    40381 acgagcaggc tgttgctttc cacgtgggat tgtttccgaa agaaatacac cagcttgtcg
    40441 ttgcgcatgg acagttcgtc cacttttgtc gtgatgtccc ggagctcacc ttccgcaccg
    40501 atttcactga aataaacggg aaagatccgg agaagctgtt tgatcagact gtacgcggga
    40561 gcgatatccg tgttgagcag agcggaaata tttttctgaa tgaggtccgt atcgcggata
    40621 aaaataccgc ccattttcaa attaataacc agcgccgaaa tcaatttttt tgtccagcgg
    40681 ggtttcatgc tgataatttc cagccaggca cgaatattca tgatatgggc cggattcacc
    40741 ttaacctgcc attcttcgtt tgaacccttg atttccggat actggaagcc gtaagcaatc
    40801 aactcgctga taaatgtatt cgcaagctca tgactgtttt gcgcgaaaac ttcccgggcc
    40861 gttatcgtaa tacaatcaaa aatggcgccg ctgaattcac gctgagattt gcttttcttc
    40921 aaaaaaccga aaatcttgcg cacaaaatca tgaaggtttt cctgctgttc ctcctgaaaa
    40981 accattttga gcgaatgatt gatttcacgc aaggcgctgg cgtgaatatc agcaaggctg
    41041 ggaacctcca tgacctgaaa aagaaatcgc agcttggtga tatgctgatg accctcgtag
    41101 gttttggatt tttccagttg atccgcaatt tgcagataac cgttcacaat ctggaaataa
    41161 tccggcatct cgagataatt tttgatcgca tcgtttttat cttcatgggt gttttgatag
    41221 gacgtttcca gtttctccat cagcatccgc atggacgcgt ggctcagtgg ccggatgaat
    41281 tgtccgtaag cagccaccga ttccggagat tcgccttccc cggcaataaa ccactgggag
    41341 ggatctggtt gttcaagcca gaactgatac gtaatccgga aaatcctgta gagcaaatcg
    41401 acgaacagcc ggttttgcag cacatcgcca tggcccggaa aatttttgat gatttttttc
    41461 gcataggacg aacatttttt gaaaataatt tcctgctgat tgatcgcatc cagaagagat
    41521 ttgataattt ccggaaacag caacagattt ctggcggttt gctcgttgct ctggttcagt
    41581 atcgtatcga tataatcgaa aagataatgg accgcgttgt ccttgacgtc cttatccgat
    41641 gacgatttga tgacctcaaa gtaaatgcaa agcatggtcg ccagtgcaga aaggccgtcc
    41701 tgatgtttat tataaatgta gaaatcgccg atggaaaccg atttcagttc ttttaagacg
    41761 tattcccagt tgacaaaagg atgatggagc tccgttaaaa gttcgtagat ttttttctgc
    41821 agaccgtaat ggttttcgac caccttgaga agcggcgcat attgatccgg aatttctatg
    41881 gttgccgccg ttctttccag attcactttt agagcaaaag aatccagttc ctcgcttttt
    41941 tttctgggat tatcttttgt tgttgccata gggggaacct tcggagagaa gcttaatcag
    42001 aatccgcgta ttttttcaag tccggaacag atcgtacgaa aaccaggggg ggcgttactt
    42061 cgaacatccg ggttgcgctg aaaaatccct aaaaaaacaa actccggcaa tcccctcgga
    42121 gcaacgagtg ttatcggagt cgatatctat cttcctgaaa cgacactatt tcttttttga
    42181 agattttttt gctgttttct ttaccgttgc ttttttaaca gcttttttca cggttggttt
    42241 ctttgctttt ttcaatgccg gttttttctt gggcgctgct ttggtttttt tcacgaccgt
    42301 cttttttctg ggtgcggcaa tggcctgtgc ttttttaggt tttgccggtc cgagattgac
    42361 cattggctct tcacagcaga taagatcgat tgtggcacaa ccacagtctt catccagaat
    42421 caccttcaat ccgcatactt cacaacccag aacgtctcca acctttgctc tcgacatttt
    42481 cttcctcctc atggagaggg gttgaacatc ttccctctgg tattttttat ttcattctaa
    42541 tcaaaaatat tattttttca agcataaatt gctaattttt ttcaaaacat agacggcttg
    42601 acattcattg atgatttgat attatcctcc ggaaaaagga atccggatca gggtatccaa
    42661 gctcaccctg acaatcatca ccaaaaaggg cagcaaaaaa tcgatcctca ataagccgct
    42721 ttcggagggg gaaaaacgcc ggactagcat atgccgcata aaaaaaacca aaagtaccgc
    42781 agctgggtgg aagttgatct cgacaacttt atcgccaatc tgaaagaaat caaaaggcta
    42841 atcggcgcca aagccgattt tatgatgacg gttaaagccg atgcttatgg acacggcgcc
    42901 atagaaatat cgaatatcgc gctgaaatgc ggcgcacgga tgctgggcgt agccaacgcc
    42961 gacgagggcg tgcaattaag aatcagcgga attgaagcgc ctgtggttat cctgggacct
    43021 tccacaaccg cggaaatcga acagatcatc aaatataatc tcataccttc cgtttcagat
    43081 ttatcttttg caaaaaagtt caataacgcc ctgatcaaat cccatcataa acgtcccttg
    43141 cacatagaag tggataccgg catggggcgc ggcggcgcaa tgcataccga agccgtaaaa
    43201 ctcgttctcg atttgtccgg attatccaac attatactgg aaggcatttt cagccacttt
    43261 gcttccagcg aaaaaatggt gtcccacaac agcacgcaat ggaaatgttt ttcggatgtg
    43321 ctggctgaaa tcgagtcccg gggaatatat attcccatcc gtcacatcaa caacagcggc
    43381 gccattttga attatcccga atataaactc aatatggtgc ggccgggaat tatggcctac
    43441 ggcatatatc cttcgccgga aaacgaatcc aaagccggac ttttgccggt gatgtctttt
    43501 aaaaccggca tcgttttgct gaaagacttt ccggcggggc acggaatcgg ctacaacagt
    43561 acattcgtta cgtcacggcc cacccgcatc gccacgattc ctgtcggcta cggggacggt
    43621 tatccgttta ttctatccaa ccagagcaca gcgctcattc atggtcaaag ggctcccatc
    43681 gtcggacgga tttccatgga tatgtgcacg gtggacgtca cccatatttc caattgcaaa
    43741 atcggcgatg aggtcgtttt gctggggcag caggaaaaag aatgcgtcag cgccaatgaa
    43801 attgccgcca aggcacaaac gatcagctat gaaatcctct gcgccctggg aaaaagagcg
    43861 ccgcgtgttt ttttgcaaaa agggaaacac aaaaccgttg aaccgcggct taagcggata
    43921 tttgtcccgg aagaggaacg atccctctcg cgtattgaca acatcattcg tcactgcctt
    43981 ctggcgcgcg cacgcaacga agaactgggc aacgccattt attatgaaat gtttgaaacc
    44041 ctttttggca acagggaccg gcgtctcgaa ctgcgttcgg gtttccgcta cgatatccgc
    44101 atcgcgaaaa tgccgaaaac gaaagatgtc gttctttcca aagactatct gcatgtcggc
    44161 atgcacgtcc aatacaaaaa gacgctgcgc gacaatattt tcatgatcgg ctgcgcctcc
    44221 agccattcgc aactcaacgc gttatttaaa gatcctcgct gtgaataccg ctggctattg
    44281 aacgacggcg gaaacctctc cgggaaacgc gactttaccg tcgaacgggt acgcatcgat
    44341 aatgaagacg ttcccatcat cgaaacaaaa agcacaaaac gcggttatga ggtgtggtgc
    44401 gggtccgacc ggctcaaaaa gaaaataaat caggatgtca aacttgaaat ggacatatcc
    44461 acaaagcagg ccagacaaaa aagaactttt cccgtttatc tggtttatcc tgtcagaggg
    44521 cttgagataa aatttgatta cgcaaacgcg ggtatcaaaa atgtccgcga ggaaagtttc
    44581 tttgcaggaa aacatccgca gccttccgta acgggaaaga aaggaaaatc catcgccgtt
    44641 aaaatatccg acgaagaatg gattttcccg acaagcggcg tcatttttat ttgggacgac
    44701 taagctcgag ggtccggtcg ttatttgatg tagtaaccgg acaccctcca ccgcccatct
    44761 ttgtcgacca tcggcgtaac ggtctcaatg cccgattttt tattctcgaa tgacgtattg
    44821 aactgaatga caacatactg cccgtcgggc gctcccggca ggg
//
LOCUS       DAOSME010000013        43389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1135_length_43389_cov_23.303411, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000013 DAOSME010000000
VERSION     DAOSME010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 43389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43389
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1135_length_43389_cov_23.303411"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLG98_03285"
     CDS             <1..818
                     /locus_tag="PLG98_03285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOE32304.1"
                     /translation="SIQGSAIVSAKIEGVLHEFSTISGVKEDVTDIILNLKGVRFKLG
                     QANIKEVSIDVSRAGEVTAADIMTDGTVEILNPEHHIATLSSGSFKAKMMVKMGKGYV
                     PAKKELDPDQPKGTINIDAVFSPIKKVNYVVSYARVGQIADYDKLTLEVWTDGSVKPE
                     DAIAYSAKILKQQLDVFINFEEVEEEILPEKQDEPDNINKILSRSVEDLELSVRSANC
                     LKNAGINTIGELVKKTEAEMLKTKNFGRKSLSEIKDILAEYDLTFGMDIGSKN"
     gene            882..1304
                     /gene="rplQ"
                     /locus_tag="PLG98_03290"
     CDS             882..1304
                     /gene="rplQ"
                     /locus_tag="PLG98_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOE32305.1"
                     /translation="MYHGKAGRKLGRTSSHKEAMLRNMVTSVIKHERIRTTDAKAKEL
                     RKLAEKMITLGKKGSLHARRQALAVIRDKETVGKLFGEMTERYRNRAGGYTRIVKAGY
                     RYGDNAPVSILEFINDEKKKEKAKPKARSRAKAKEKAE"
     gene            1441..1602
                     /locus_tag="PLG98_03295"
     CDS             1441..1602
                     /locus_tag="PLG98_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32306.1"
                     /translation="MGFNGGELTVMTLPLALGRPGSLSMIQQTQALKTGNEAVLDIID
                     CPVRVYAFR"
     gene            1624..1884
                     /locus_tag="PLG98_03300"
     CDS             1624..1884
                     /locus_tag="PLG98_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32307.1"
                     /translation="MAEKKIGEVMKFFSKPSVAAVKITDGEIAVGDSLKFSGHTTDFT
                     DVIESMEVDNQSVPKAVAGDFIGIKVSDRVRPGDEVFKVIPD"
     gene            2005..2829
                     /locus_tag="PLG98_03305"
     CDS             2005..2829
                     /locus_tag="PLG98_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOE32308.1"
                     /translation="MKSRIAEAIKLKYQPVALLWSVEKPADAMQFAEGKWGCVMWLAV
                     HAAKGRAAVADRKTFGCFGGGVGLGFGNQYKNFPGGEEGFCYFLSSGNARRPGGPEMA
                     AKVKPFMTRESHDNFLNGERYVQNPQLVEKFIRDLPLTDIPKPFVVFQPLSDVDQDGE
                     KPQTVIFFVNPDQLSALTVLANYGREGNENVIIPYAAGCQAIGIYPYREAGLKNPRAV
                     VGLTDLSARVYVRKQLGESHLMTFSVPFNLYDEMEQNVPGSFLERHTWQSLLSENR"
     gene            2917..4227
                     /locus_tag="PLG98_03310"
     CDS             2917..4227
                     /locus_tag="PLG98_03310"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOE32309.1"
                     /translation="MKNSRTSQYLDSLNINKMRFGLGAITSVLSRLGHPQKSFKAVTI
                     TGTNGKGSTAAMIAAMLHKAGYCVGLYTSPHLIDVRERITVNGRKISRKDFDRIIVCV
                     RKKVVQPLTYFEFITAVAFEYFKTRKADIAVLEVGLGGRLDATNVCRPLVSVITNIAF
                     DHTAYLGRTLTSIAVEKAGIIKPHGLCVTGVAQKKILGLLQDICLQRGASLYRLNHDF
                     RLARQKNGSLLYEGVYSDVKNLTVPLRGEHQLANAALALAAVEMCARKGFPVSETAVR
                     AGLQNVRWEGRLEVLHISPWVLLDGAHNPAGIGALCRSLKKDFSFRHLILIFGVLADK
                     DYRKMLQKIVPLARVIILAPLKAQRAASVAAVSQTIRSLGRMPVVSQSVHQALELGLS
                     LAGSRDLICATGSFYLAGEVKQTFSKLVFCDNEKSKKVQGKKVS"
     gene            4224..6566
                     /gene="lptD"
                     /locus_tag="PLG98_03315"
     CDS             4224..6566
                     /gene="lptD"
                     /locus_tag="PLG98_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOE32310.1"
                     /translation="MNINTLINVKPIKHNGLASFSGCLEPVFTFCLVAVIIILWSFPA
                     DCAGKQKSSAGKASPSIQKKNFPASVHTNEKSPVSIEADAMDYDHVSDLYRARGQVSV
                     FYSGASLYADEVELDNQNHVARAQGNTLLKIGEDTLKGERLWFNIEDKTGAAFNADAF
                     YARNHFRVRGNIIEKTGENTYFIDNPSATTCDGSNPDWEIAGSEMKVTIEGYGLMKNA
                     CFKVGSLPLLYSPYILFPAKTKRQTGFLLPYLAYSRDKDGIDVELPFFWAVSPWMDAT
                     FYQRFIEKRGFKEGAEFRYDFGNKSFGTFYGDYLEDTKQVTETEDDALRRDWQGMHKR
                     WSYFLHHQTRLDSQFYLRTDLKKVSDKWYFRDFSSYNYYREHFNSTSEDRFRNVSFEG
                     DKSLRYLDSTIRVYKGWSKYNVTGLMHAADDFAAVNNDQTLQRYPEIIFAGVKQPLLH
                     TPFYYELAGRYDYLYKKEGHKGHFADLSPSLSMPVNLSDYVRIIPQFTFKETFWSRDD
                     DENGLKEKTADRSVYNASVSFGSQLFRVFHLNGKHWEKIRHEIKPEIIYSYMPSISAE
                     DVPDYYLPALSPFGMPLASLSSDPLNGQNAVAWSLTNTLTARVKDQGGSLSYLEFFRL
                     KVFQAYDIDEARRDMARNAPERRPFSDMGIEFDFSPHKYFSFRMRDKYNVYSGWKQNS
                     YDLSLRDWRGDALLVAYRNTEGLVEEINLGVKAVITDHFYSTFVLKRDLQDARKIENS
                     LGFVYHTQCWSLGFDLSDTDDDVRFLFRISLAGFGKTADK"
     gene            6663..8180
                     /locus_tag="PLG98_03320"
     CDS             6663..8180
                     /locus_tag="PLG98_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32311.1"
                     /translation="MDTFILVATSFTIAVSLMITGKTNRLQSAFAALCAAVFVSQAAV
                     TLENIFHYEFLLKIKYLGSLAIAPAALRFFHHLTKGQSIILRNFFSLFAVISLCGVVF
                     VFLPLSEKTCFHNVIAAYVLFSLMICYLALLGHVGTLPSGTEKRRFGYLLVFCPVALI
                     LSYLDLLNFWGFSLLKINGMVSSALLYFILLIIAYPQLLELHDFLARSLVILLSTLAG
                     AAIFYFTVSFFNGDPPLTGLLMASFLVVISLSPMRMIFRKIFGVFYPDSKDIFTSLYE
                     FDEKLEREKALMLEEMAPVLAHEIRNPLGSIKGAAQYLKTEASTGEHKELLDVIVEGT
                     DRLNTVVSKFLDYARPYQLQLKTQDINAIIRRAISVIAANKLLEHITIVEDLEDKLPF
                     AAVDEQQCLQVVLNIALNAIEAMPQGGTLTFCTSSQKSETAEDLALTIRDTGTGIDQK
                     YVKDIFKPFFTTKERGVGLGLAICQKIIREHNGRISVQSIASQGTTVVIQLKRSG"
     gene            8234..9607
                     /locus_tag="PLG98_03325"
     CDS             8234..9607
                     /locus_tag="PLG98_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE32312.1"
                     /translation="MKKILIVDDELNMRLVLSAMLKKEGFEVSSASNGREALQILQSN
                     KMALVITDLKMPGMDGMDLLTQISANHPQIPVVMITAHGTIATAVEALKKGAFDYITK
                     PFDLDDLKNIVSKAIRTSTLKESEFSMAPSEIDRLGIIGTSPAIMEIFEAIHRVAQTT
                     TTVMITGETGTGKELVADAIHYNSQRKNNPLIKINCAAIAETLMESELFGYEKGAYTG
                     AAVTKPGKFELAHKGTLFLDEVAEIPREMQVKLLRVLQEQAFERVGGLRTIKVDVRII
                     AATNQHLLRQVQTGNFREDLYYRLNVFPIDIPPLRERKDDILSLADYFLDKFNKKLGI
                     SVGMDNEVKDILLRYDWPGNIRELENLIERMMLLARGCRIMSGDVPDDFKKSVDHVNT
                     ALSPGIKKPFKEYVRDQVENVERQMIARALDESGGNITQAAKKMGLSRKGLQLKMSKY
                     HLRKESK"
     gene            9648..9857
                     /locus_tag="PLG98_03330"
     CDS             9648..9857
                     /locus_tag="PLG98_03330"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOE32313.1"
                     /translation="MNFKIGLVAIFALLALIFLVQNIEVMTVHFLFWQLSMSRAIFLF
                     FILLTGFIIGWFLHSFVSYSQKKKN"
     gene            complement(9908..10750)
                     /locus_tag="PLG98_03335"
     CDS             complement(9908..10750)
                     /locus_tag="PLG98_03335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOE32314.1"
                     /translation="MNAKSALIIAAIVFIFTGCASENIQVRKLPDSAINAGLEDLRQE
                     VADNGVPGEWFDDQMDHYAFRLHHNIDRFFRKSAEKKTDSEKKRSLLWYFSIFGVETK
                     IEKGKTFIEDYTGILERAEAKHGIHKELIAAIIGMETNFAQRRYRGKFYAFNSLVSQY
                     IFTDRKKFAVREITSLYKFSEKTDRSPWYFKSSYAGAIGWGQFIPSSLLSFFIDENGD
                     NDDIDPFSIEDTIFSVENYLYRHGLSGKNVHKRRSRYKAVYAYNHSDAYVQAVLHIYD
                     GLKN"
     gene            10937..11722
                     /locus_tag="PLG98_03340"
     CDS             10937..11722
                     /locus_tag="PLG98_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE32315.1"
                     /translation="MHLTGAMFHSRKYPTKDHYPFHLPLFRATKRLVFDTPVTLFVGE
                     NGTGKSTLIEALAQACQIHIWRDTQRTRPDYNPYEDQLHRYMSVIWANEPVPGSFLGS
                     KNFQYFAEKLDEWASADRDQLNYFGGRSLTTQSHGQSLMSFFYSRYRIPGLYLLDEPE
                     TALSPQSQILLLDILQRMSAAGHAQFIIATHSPLLLACPQATIYNFDRSPLTKIVYEK
                     TKHYRIYKDFLLNRSNYLKPKAKHQRKDTKCLPKKKSLTKQSA"
     gene            11680..12810
                     /locus_tag="PLG98_03345"
     CDS             11680..12810
                     /locus_tag="PLG98_03345"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOE32316.1"
                     /translation="MLTKEEIIDEAKRLKFADIGFTGAEPFCSQKDYLLTHQEEYGWA
                     EAIGLGLLAGTDPRNILAGAKSIIVVLESYFEESFPQPMERHFGRCYLDDDRITQDGL
                     ALKIKAFRKYLKANGVDSKVPFHLPHRLSAARAGLGTFGNNCIFYARRVARGSSFVFP
                     IALIVDCEFTPDEPSVAMGCPDWCRNACVAACPTRALKGGGKIDPRRCISFLTYYGSG
                     LTPQDLREPMGMYIYGCDRCQNVCPRNIPWMAEEKPVNQRVSAKASDFGLSRLLHMDA
                     VYFEKKIWPHMFYMSPDDLWRWKMNVARAMGNSLNPVYVADLIRAFHENSDERVRCMA
                     AWALGRIGGNEAVEALGRFLKNSDGVVREEIEQAMVSASAGV"
     gene            complement(12821..13300)
                     /gene="greA"
                     /locus_tag="PLG98_03350"
     CDS             complement(12821..13300)
                     /gene="greA"
                     /locus_tag="PLG98_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOE32317.1"
                     /translation="MEKVPITKAGFEKLKKELETLKNVSIPENVRDIEVARAHGDISE
                     NAEYSAAKERQSFLYGRMQELENQLAMSNVISLKGLNTAKVVFGCYVSLQDIDSGEKI
                     KYQLLGPYESDISRNKISVTSPIGKALIGKNVGSEINVKTPGGTRNLEITDISVEEE"
     gene            13472..>13791
                     /locus_tag="PLG98_03355"
     CDS             13472..>13791
                     /locus_tag="PLG98_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32318.1"
                     /translation="MMLDKYSREINYLRVSITDRCNLRCIYCRPKEGVALHGHEDILR
                     YEEMIRVISLAVKMGLIKVRLTGGEPLVRRGFVDFVADLNKIEGLKDISLTTNGILLE
                     QFAQG"
     assembly_gap    13792..13891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13892..14622
                     /locus_tag="PLG98_03360"
     CDS             <13892..14622
                     /locus_tag="PLG98_03360"
                     /inference="COORDINATES: protein motif:HMM:NF018201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32319.1"
                     /translation="KDISLTTNGILLEQFAQGIFDAGIRRINVSLDSLNPDKYGQITR
                     GGRLEDVLRGIARAEEIGFSPIKINTVAIKGFNDDEALDFARLAQEQPFQVRFIELMP
                     VGKTTLDYGEDYMPGSQLIKIIRQRYALEPLKNKKNRSDGPAKIFKIKGGRGEIGFIN
                     PVSDHFCSTCNRMRLTSDGKLRACLLNEAEVDLKKALRENCSDAELEKLIWDAVLLKP
                     RQHDIVCTENSLRKCSRNMSEIGG"
     gene            14756..15538
                     /locus_tag="PLG98_03365"
     CDS             14756..15538
                     /locus_tag="PLG98_03365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE32320.1"
                     /translation="MFFDQKKTGFPAERLKTPRDDYIFSRQKELVLDLITPLAGERLL
                     DIGCGTGNHLDMFRGKWCSLTGLDSSGANLEIARQKHSERAELILADPEDIPFSDNEF
                     DIVTMIHCLETAVHPQKIIDEAVRVCRGRVFIGCVNNYSFVGTRQRLKEMFGFSFSQK
                     IRFFSLPEIRKMAEASAGNNAMKWGSVIYFPAMVYDFFEELEEMLPFRKNPFGAFVGL
                     VCPVTYTYRTAQNPVMNSFNIKAETRKTAPEAVRNMLKEADR"
     gene            15535..16545
                     /gene="amrS"
                     /locus_tag="PLG98_03370"
     CDS             15535..16545
                     /gene="amrS"
                     /locus_tag="PLG98_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOE32321.1"
                     /translation="MIKEAMLYEKLQDGNVRCNLCAHRCTIHPERQGVCGVRGNRDGV
                     LYSMAYGTLIAQNVDPIEKKPFFHVYPASRSYSIATMGCNFFCDFCQNHDISQAPRKT
                     KMIYGEDSMPSDIVERAKESGAKTIAYTYTEPTVFFELAYDTAKIAAASGLKNVFVTN
                     GFMTPEAIEIIAPYLSAANVDLKSFRDEFYKKRCGARLVPVLDSLKKMKERGIWVEIT
                     TLLIPSLNDSEEEMRDIAQFIAGLGRETPWHISRFHPQYKMQHLPVTPLESLHRAGQI
                     GREAGLKYVYSGNVPGDDAENTRCFHCGKLLIERYDFKIIGTNLQKNKCSHCGTQLEG
                     IF"
     gene            16859..17020
                     /locus_tag="PLG98_03375"
     CDS             16859..17020
                     /locus_tag="PLG98_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32322.1"
                     /translation="MTEEIKQEQPATDPHEQENQPITLKEKIAAAVLIAYVISLIWLA
                     GSKHWFGLW"
     gene            17299..19227
                     /locus_tag="PLG98_03380"
     CDS             17299..19227
                     /locus_tag="PLG98_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE32323.1"
                     /translation="MRFEHLFSPFTINGMTLKNRAVMPPMATGYGNADNTVSERLLAY
                     LERRAQGGTGLIITEVCAVDPRGKGFPNEIGVWSDDFIGALSRIPAALHRHGAKAALQ
                     LHHAGRETFEAVLGKKPEAPSAIPSAVLGQPCEAMSLERIAEIVRAFAQAAGRARQAG
                     FDSVELHGAHGYLLNQFLSPFSNNREDEYGGSEENRMRFILEVIAAARKAVGDDFPLW
                     IRISADEMVRGGYDFSLMRRLAPRMVAAGVDAIHCSVGVYSTPGGLSIASMDTEPGFN
                     LFRARALKEVVSVPIIGVGRINDPAMADQAIARGDADLISFGRQHLTDPDFIEKARRG
                     SLEDIRRCTACNQGCIERLSFEMKSTTCSFNPECGREYKGPVLPAGTPKKVWVIGAGP
                     AGLSAALVLAARGHQVEVFEKESQPGGQLLSASRPPHKAVFMDWVNWALRRLKQWSVP
                     LHCSHPVSADDIKKAAPDAVIVASGAYPVTASIPGLKSELVADARDVLTGNIKLAGPA
                     VVLGAGYVGMETADYLLANGIAVTVLEMQTLPPVNRFSAHGYWLHKRIKDSGGKIIFG
                     ARVLGIENNIVRYVQADKPSEEKASLIVTAMGAKSENTLEDVLNALAVPYRVVGDARS
                     PRRILEAIHEGYKAGEEI"
     gene            19381..19824
                     /locus_tag="PLG98_03385"
     CDS             19381..19824
                     /locus_tag="PLG98_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE32324.1"
                     /translation="MQRVSDILKSKSKQMWTINPNDTVFEALKLMAEKEIGALVVVDK
                     KGKVAGIITERDYARKIVLKGKTSPKTLVKEIMTPFNKMYTVKPDTSMEGCMVLMTGK
                     HIRHVPVFEGSKFLGIVSIGDVVKSTISEKDVLINQLSNYIAGKY"
     gene            20087..23635
                     /gene="smc"
                     /locus_tag="PLG98_03390"
     CDS             20087..23635
                     /gene="smc"
                     /locus_tag="PLG98_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOE32325.1"
                     /translation="MNLKNLEISGFKSFRDRVVLDFSNGVTGIVGPNGCGKSNIVDAI
                     RWVMGEQRVKALRGKKMDDVVFNGSQDSAPVSMAEVTMTLYANGQSFPGAYSDLNEVS
                     ISRRVVLDGDSEYFINKVPCRLLDIKEFFMGTGVGARTYSLVEQGHVTNLVEAKPEDR
                     RLYIEDAAGVSKYKSRKEAAVRKMDLTKQNILRLNDIIKEVKTQLNTISRQAKKAEQY
                     KKIKQEIREAEITVALQTYVDFMARESDLQQQRTELQQQEALIRADFQAKESMLDEMK
                     VKLLENEELTAKVQQELYEIKNTISIKEKSIEFAQRQVTDMSGRREKDLTEIQVLQTR
                     KADLAAEIENLRKNSAEAENKIVALKAEHESLQQKVQELTEADKALSSQLEEKKILFI
                     DVVTEKAKLHNMISSLSKNIEDLKKREEKETRELEEDQKMMADVCARLEAADEALKKE
                     KADIVGLEAKMAEAATELDRFRSDLQFNEEDIARLREDTSVKSTRLISLREFQESSKW
                     SNEGVKTIIENKEHSDKFYGVIADHISVPREYEAAVEAVLGEKLQYVVVQSQEDGVRA
                     IDYLKNYQLGRGSFISVDLRTHDARTYSEAHLNEAKPLLDKVTVRDDFKPIIDCLLGD
                     VLLIPTIENGISLWKKNGFRGTFVTPEGDIISPHGVLTGGSGSAVEKSLLATKREIGE
                     LEAEIAALSLDLETKVGRRTKILATISRWEEESSQMKTEIHALEITVNGRKKDRERFD
                     DDVNRLRQRIAVLEFNRQTLNTEKTEAEEKLRRFNEDVLQKDGEEKQINDIIAALNVQ
                     KEQSRVQIDEQERYVTAGKIELASLEEKKEGDVRTISRLQDHIDSMEQEQRTKEEDIV
                     FCEKQIAELTQGIENDQVLLKEIYSAFAAQEALLAEKKAEQNREDAQLKILEGEIREI
                     KKNLDELRQQVNAMEFQSREITLNRENLRKSVAEKHDVDLAGMAADFEKVTEEKLVEI
                     VDALEKNKQMLETFGEVNLLALNEYEELDQRYNFLSTQIADLNASINVLQRTIARINK
                     ISRARFAETFEAVNACFKEVFAQIFPGGRGELLLTDENDMLETGVDIDIQVPGKRKQN
                     VSLLSGGEKSLVAVALIFAILKYRPSPFLVLDEVDAALDDANTNLFNRLIKDVAGKSQ
                     VVMITHNKSTMEVADTLFGVTMQKQGISTLVSVNLN"
     gene            23702..24622
                     /gene="ftsY"
                     /locus_tag="PLG98_03395"
     CDS             23702..24622
                     /gene="ftsY"
                     /locus_tag="PLG98_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOE32326.1"
                     /translation="MAKSSLFEKLKAGLVKTRTALTKSLDNLVFGKRVLDQELFEDLE
                     ELLISADMGPQFTYDLIDDVKQRVSRNELNNAQEIKKVLQERMTAILQKMEKPLIIPR
                     GKPFVIMTIGVNGSGKTTTIGKLANILKSEGHEILLVAADTFRAAAVEQLEVWGARVG
                     APVIKHKTNADPAAVVFDAMSKVKAGFNGVVIVDTAGRLHTRVNLMEELKKVKRIINK
                     ELPDVPPEILLILDATTGQNAVAQAKTFKEEIGATGIVLTKLDGTSKGGVVVRIASEL
                     ALPVWYIGVGEGVDDLRVFESSNFTKALLD"
     gene            24650..25294
                     /locus_tag="PLG98_03400"
     CDS             24650..25294
                     /locus_tag="PLG98_03400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOE32327.1"
                     /translation="MGKIYAIGDIHGCLTKLKTLISDIDIDFKKDTLVFIGDYVDRGE
                     FSRDVVDYVSGLQDSHENIVCLLGNHEQMFMNYLEGVDEEIYLRNGGMQTLRSYEIFL
                     SDDIKERKRKMSARHRKFFETLLPYYETEKYIFVHAGLRPGIPVAEQSQNDLLWIRYE
                     FINTEKDFDKTVVFGHTPLMTPLIDKNKIGIDTGAVYGGKLTCIEFPGLKIYQA"
     gene            25297..26199
                     /gene="ftcD"
                     /locus_tag="PLG98_03405"
     CDS             25297..26199
                     /gene="ftcD"
                     /locus_tag="PLG98_03405"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOE32328.1"
                     /translation="MKIIECVPNFSEGRDDKKILSIVSVFKMFPGVRLADFSGDADHN
                     RSVFTFLGKPDDVLDATLAACGKALELIDMRQQSGVHPRLGAVDVVPFIPLGRAKMKD
                     AVELAHAFGMRFSQRFGVPVYFYGYAARNGRNVELPDVRRGGYERLADKMSSTESLPD
                     AGENQFNARSGATAVGARDLLVAYNINLVSDDLLLARHIASRIREKGGGLKNVRAIGV
                     MLKRRRLAQVSVNLTDCKETPLKTVFDRVKALAAEGGAQILESELIGLAPKCAFAGAT
                     PEYLRLKDFDENRLLETHIRRLSE"
     gene            complement(26201..26482)
                     /locus_tag="PLG98_03410"
     CDS             complement(26201..26482)
                     /locus_tag="PLG98_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32329.1"
                     /translation="MNDLLAYISNWSVLKWVVLVLIAGFIGQFGRMAAEAIAKKIRAG
                     RPQQENKTIKPETPLQTPAPQPVSSGDLPDKKLLKAIAKAKKKEAKKKE"
     gene            complement(26493..27107)
                     /locus_tag="PLG98_03415"
     CDS             complement(26493..27107)
                     /locus_tag="PLG98_03415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE32330.1"
                     /translation="MKLFLFDFDGVLVDSLDVYEKTVKDNLQKIKQPVVQSREEFLEL
                     FEDNFYTSLKNKGIDLNVFMKAAEDILAQVRITDMKPFPDVAPVIETLHKNHCLIVVS
                     SNDYASIKEALDLFHFNDYFRDILGSDFMFSKKEKILYAAEKYHVEPNKIYYIGDTTG
                     DIKEGKQAGVKTVGVTWGWHSRERMAAAEPDYLFDTPGELLQLS"
     gene            complement(27104..28153)
                     /gene="mtnA"
                     /locus_tag="PLG98_03420"
     CDS             complement(27104..28153)
                     /gene="mtnA"
                     /locus_tag="PLG98_03420"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOE32331.1"
                     /translation="MIKTIFWKNNAVVLIDQKALPLAEKYIVCKSYKAVIACIKDLTV
                     RGAPAIGVAAAMGAALGAIHLASLSPDKFRKKFYAICDEIAQARPTARNLFWALERMK
                     KIVSHSSQLSQRDIVKELIKKAKHICSEDIAINQQMGKNGSRIIADGDNILTHCNAGA
                     LATAGYGTALGVIRAARKQGKKLHVYVDETRPVLQGARLTAWELKKEKIPYTLITDNM
                     AGFLMQQKKIDKIIVGADRIAANGDTANKIGTYSLAVLAQAHGVPLYIAAPLSTIDVS
                     LKTGDSIPIEERKSDEVAHLQGTRSAPRGTIVYNPAFDITPARLITAMITEKGILRKP
                     FRASIARLYKGERGS"
     gene            complement(28146..29588)
                     /gene="gatB"
                     /locus_tag="PLG98_03425"
     CDS             complement(28146..29588)
                     /gene="gatB"
                     /locus_tag="PLG98_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOE32332.1"
                     /translation="MDFETVIGLEVHAQMLTDTKIFCNCSTKFGAAPNSHTCPVCLGM
                     PGVLPVLNKKVVEYALKMALATNCEINKSCEFARKNYFYPDLPKGYQISQYAYPLAEH
                     GYVMLETNGEQKKIGITRIHMEEDAGKLLHDEHNPVSYVDLNRTGVPLIEIVSEPDMR
                     SPEEAADYLKRLHEILVYLEICDGNMEEGSFRCDANVSIRPKGQKAFGTRTELKNMNS
                     FRNVQRALEYEIKRQQYLVENGGTVIQETRLWDDAQGATNSMRSKEEAHDYRYFPDPD
                     LVPILVDEAWVEKIRKELPELPLAKRERFIKDYQIPAYDAGVLTADKALALYYEKVVK
                     LCNKPKIASNWLMGDVMRFLNEDKRSVRECPIKAESLADMIRLIEEGAISGKMAKEVV
                     EDMYKTGKSPQNIIKEKGLVQITDRDALVKTASITVGKYPQQRDDYRGGGREKLFGFF
                     VGQVMKETKGKANPQLVNEILKEILDKKDD"
     gene            complement(29588..31045)
                     /gene="gatA"
                     /locus_tag="PLG98_03430"
     CDS             complement(29588..31045)
                     /gene="gatA"
                     /locus_tag="PLG98_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOE32333.1"
                     /translation="MKLNQLTIHELQKRIKTGETSATQITHDVFARINAVDEKVHAYI
                     RLMQEEAFQAASKADEDIKQGDIRALTGIPIALKDIVCTKGIPTTCGSRILHNFIPPY
                     DATVVERLRKAGAIFVGKANMDEFAMGSSTETSYFGATRNPWDLERIPGGSSGGSAAA
                     VAADECIASIGSDTGGSIRQPAALCGTVGMKPTYGRVSRFGLIAFASSLDQIGPFTKD
                     VEDCAIMMNVLAGYDPKESTSVNEEVPDYRQFVGRDIKGWKIGIPKEYFIDGIDPGVH
                     AAIKKAITVIEQSGGQCVDISLPHTQYCVATYYIIAPAEASSNLARYDGVRYGLRAQD
                     VRDLLMMYKESRKQGFGAEVKRRIMIGTYALSAGYYDAYYKKASQVRALIKKDFDKAF
                     KICDVIMTPTSPTPAFKIGEKTDDPLQMYLSDIFTISANLAGIPGISVPCGFSSSGLP
                     IGVQFLASHFEEGKLIQIASAYEKNAKIEKRRPAL"
     gene            complement(31107..31394)
                     /gene="gatC"
                     /locus_tag="PLG98_03435"
     CDS             complement(31107..31394)
                     /gene="gatC"
                     /locus_tag="PLG98_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOE32334.1"
                     /translation="MKISSREVEYVAHLARLEITDAEKEKFTAQLNDILLYIDKLNEL
                     DTGDVKPISHAISITNAFREDKISNSIGTREALANAPDTRGEFFRVPKVID"
     gene            complement(31410..32468)
                     /locus_tag="PLG98_03440"
     CDS             complement(31410..32468)
                     /locus_tag="PLG98_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOE32335.1"
                     /translation="MSRKTGVVKDGKYMQHSMGFAHPESPERLAVIYEMLENPLMSWK
                     FTPIEPREATHEEIERIHARSYVDFIASTAGKRSVFLDPDTSTSPETYDAAKLAAGGV
                     CNAIDSVMKGDVDNAFAFVRPPGHHAEKDTAAGFCVFNNIAIGAMHAIQKHNLKRILI
                     VDWDLHHGNGTQHSFYGDQRVLYFSTHQYPYYPGTGSISETGRGQGEYYTINVPLTMS
                     VGDAVFVKIFRKILEPVALEFKPELILLSAGFDTYYQDPLGAMQVTPEGYAAMTRILL
                     NIADTCCQGRFVAVLEGGYHLSGLVKSVKAVLNEMLDETHFSEERLSLMEKDVDERTT
                     ESVIDKVITRINPYWKVF"
     gene            32705..32965
                     /gene="tatB"
                     /locus_tag="PLG98_03445"
     CDS             32705..32965
                     /gene="tatB"
                     /locus_tag="PLG98_03445"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOE32336.1"
                     /translation="MFGIGMQELIIIAIIALLVVGPKKLPDLAKSLGKGFSEFRRAAD
                     GVTEDIKDAVKVDEKPKNDDEWKNSLLMKKPDHDNVKSDSSE"
     gene            33027..33791
                     /gene="tatC"
                     /locus_tag="PLG98_03450"
     CDS             33027..33791
                     /gene="tatC"
                     /locus_tag="PLG98_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOE32337.1"
                     /translation="MDNTQAAEDQKMSLTDHLLELRKRLTRSLIALAAGFGVCYYFKD
                     LIFDVMTRPLTQVLSKDSHLVYIGLTEAFFTYMKIAFFASLIITSPYILYQVWKFLSP
                     GLLPKEKQYVVPFVISSSLLFIGGVLFGYFIVLPTAFKFFVSFNNEYLQAMLSFKDYL
                     SLFVTFLLGFGLSFELPIFIFFLTKLGIVNAAKLSRQRRYAILVIFLVAAVLTPSPDA
                     LSQILMAIPLMILYEISIFVSKFAETKKEESQNDEG"
     gene            33800..35824
                     /locus_tag="PLG98_03455"
     CDS             33800..35824
                     /locus_tag="PLG98_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOE32338.1"
                     /translation="MFLNRKKSNRKNDGEAKKLSSLFNFFLGFVVFILVMTVFGVIGF
                     YAFTMDLPGIDALKDYRPSISSRVYDDKNELIDEFFLEDRKLIKIKEVPRVVVQAFVA
                     AEDSRFFAHKGFDLQSIFRAVFKNIEAGHIIQGGSTITQQVAKMMYLSPEKKYTRKFK
                     EAILAYKIDRYLTKDEILNLYLNQIYLGHGTYGIESASLGYFGKSARELKLHEAALLA
                     GMPKAPSNYSPFLHYDKAKQRQYYVLTRMKEDGYISQEEMEKAYAAPMNLRSIRPKDK
                     MAAYFVETVRRYVQEKYGADVLYKEGLSIYTTLDLTAQKYAGEAMERGLKELEERNKY
                     KSGLVQGALFCMDVRTGAIRAMVGGRDFNQSEFNRATQSRRQAGSAFKPIIYTAAFDK
                     GMTPATVIDDSPLAVDDVSQPDGIWRPKNFDDKFMGPITMRTALVLSRNVVTVKILQE
                     IGIDYAVSYAMNMGITSPLVRTLSLALGASGVTLQELVQAYGVLANQGQKVSPFFIKK
                     IVDRTGNVFEETKVQPEQVIDPRIAFITTHVMQDVVTSGTGTRVRSIGRPVAAKTGTT
                     NDTRDAWFIGMTPSLITGVWIGFDQEASLGSQEVGGRAAAPIWLYFMEKALQNTPVES
                     FPVPEGIVFEKINPKTGEPAGPSEKGTNEAFLLGTSPSSTLGDSIKGLFR"
     gene            36406..38109
                     /gene="ilvB"
                     /locus_tag="PLG98_03460"
     CDS             36406..38109
                     /gene="ilvB"
                     /locus_tag="PLG98_03460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOE32339.1"
                     /translation="MKLKGTEILLKALEEEKVEIIFGYPGGAVLDIYDQIYKSNMKHI
                     LVRHEQGAVHAADGYARSTGKVGVCLVTSGPGATNTVTGIATANMDSIPLVVFTGQVP
                     TALIGNDAFQECDITGITRPCTKHNFLVRNIDDLAATIKEAFDIARSGRPGPVLVDLP
                     KNVMAAQTEYDPANIKIRNHEAAYKPAPKKMAGLLDLLTAAKKPLIMTGGGVILGRAS
                     SLLTEFARKYQMPVTGTLMGLGSFPGTDPLWLGMLGMHGTYYANMAISHCDLLIAVGV
                     RFDDRVTGTIDTFAAKAKIVQIDIDPSSINKNVAVDLPIIGDTQAVLKDLITFLDEHN
                     YAYDKDARNEWLNEIAGWKEKVPLTYCQNGNVIKPQSVIETLYKLTGGDAIITTEVGQ
                     NQMWTAQFFPFDKPNTFISSGGLGTMGFGFPAAIGVKCAFPDKHVVDIAGDGSIQMNI
                     QELATAAQYKINVKIVLLNNGYLGMVRQWQELFYEKRYSFTDMTYAPDFVKLAEAFGV
                     VGLRATKPEDVEPVLKEGLAIDKPVLMDFRVSREECVYPMVHPGDSISNMSLGSREMI
                     N"
     gene            38278..38763
                     /gene="ilvN"
                     /locus_tag="PLG98_03465"
     CDS             38278..38763
                     /gene="ilvN"
                     /locus_tag="PLG98_03465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOE32340.1"
                     /translation="MEKKEHIISILVHNKPDVLARIAGILGGKGYNIESLCVNSTTHY
                     EVSKIVMTTVGTQDTINRIEGQLRRLVDVIQVDDLTNVESIHRELILVRLNLAADKKE
                     QIKKAVDMNKWKVIVNADTYVILEITGDRSQIDFALARLEPLGIADITRTGIVALNLE
                     N"
     gene            38795..39853
                     /gene="ilvC"
                     /locus_tag="PLG98_03470"
     CDS             38795..39853
                     /gene="ilvC"
                     /locus_tag="PLG98_03470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOE32341.1"
                     /translation="MAKINFGGVLEDVITAKEFPLKKAQKVLKNDVVAVLGYGVQGPA
                     QAANMKDNGINVIIGQAKEDTRYWKKAIADGWKPGKTLFPIEEAVKRGTIIQYLVSDA
                     AQKLLWPKVKANLKEGDALYFSHGFSIVYRKQTGVIPPKFVDVIMVAPKGSGTSVRRN
                     FLDGSGINSSFAVQQDYTGRAMERTLALGIAIGSGFLFPTTFQNEVYSDLTGERGVLM
                     GALAGIMEAQYNELRKHRHTPSEAFNETVEELTQSLIRLVAENGMDWMYANCSTTAQR
                     GALDWRHKFRKAVAPVFAELYKSVASGKETAIVLKANSAPDYRVKLEKELKQMRDSEM
                     WKAGAAVRELRPEKRKKK"
     gene            39952..41613
                     /gene="ilvD"
                     /locus_tag="PLG98_03475"
     CDS             39952..41613
                     /gene="ilvD"
                     /locus_tag="PLG98_03475"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE32342.1"
                     /translation="MRSDLMKKGFERAPHRSLFKAMGYTDEELARPLIGVVNSANEII
                     PGHIHLDLITQDVKAGIRMAGGTPVEFPVIGVCDGIAMGHEGMKFSLASRELIADSIE
                     IMAMAHPFDALVFIPNCDKIIPGMLMAALRLNIPSIFISGGPMLAGKFQDKTVDLISV
                     FEGVGAVRAKKITAAELKQLEDCACPGCGSCSGMYTANSMNCVTEALGLGLPGNGTIP
                     AVLAARHRLAKRAGMKIMDLLKKNIRPRNIATLAAFKNAIAVDMALGCSTNTVLHIPA
                     VAHEAGIKLDLDLFNRISEKTPNLCKLSPAGRHHIEDLDAAGGIQAVMKVISALGVID
                     EKAITVTGKTVGGNLKDARVLNTDVIRTLKNAYSQQGGIAILRGNLAPDGAVVKQSAV
                     APAMQKNEGKARVFDCEDDAIKAILGGKIKGGDIVVIRYEGPKGGPGMREMLSPTSAI
                     VGMGLDKTVALLTDGRFSGGTQGAAIGHISPEAAEGGPIALVKEGDIIAIDIPAKKLN
                     LKVGAQELAKRRKALKPFKPRITTGYLARYAKMVTSSSTGAIFKD"
     gene            complement(41867..42307)
                     /locus_tag="PLG98_03480"
     CDS             complement(41867..42307)
                     /locus_tag="PLG98_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32343.1"
                     /translation="MTIHSYSKLEIATQQLDTALMLYFEDKNYFSVITLAGAAEEILA
                     VYLKLHKQPNAFEEMLDSSLLIGELLYNSKGSRDSMYKTINRVKNGSKHMKGRDDINI
                     QCNLKEEAQNILDRAVSNFYTLMTFENLNETSLMKKFNSQLVSK"
     gene            complement(42369..42749)
                     /locus_tag="PLG98_03485"
     CDS             complement(42369..42749)
                     /locus_tag="PLG98_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32344.1"
                     /translation="MSSDFSLEFKERYIHLIHAPDYEISPESNDILWAELAKACRKYK
                     CLKVLTEGSNQKRRITSMDAYQSGAQAAESIPGMTLAICIDDYTPDDITQFFKNVAYN
                     RGARIEFFNDKKKALAWLGFEDTE"
     gene            complement(42964..43269)
                     /locus_tag="PLG98_03490"
     CDS             complement(42964..43269)
                     /locus_tag="PLG98_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32345.1"
                     /translation="MKEIITATDDHTVESSLGWRVDILSMDALRYQERDKTITFEIED
                     YSDAIGELEWTIYIPPICKWQDENHPEEIIGQEKLDDIIDRISTAFWKLDMKIRGIA"
BASE COUNT        11254 a         9968 c        11046 g        11021 t
ORIGIN      
        1 cttccataca gggctctgcc attgtatcag cgaaaattga aggggttttg cacgaatttt
       61 cgacaatctc cggtgttaaa gaagatgtca cggatattat tttaaacctg aaaggtgtgc
      121 gctttaaact ggggcaggcc aatatcaagg aagtgagcat cgatgtcagc cgcgcaggcg
      181 aagtgactgc tgcggacatt atgacggacg gcacagtgga aatcctgaat cctgagcatc
      241 acatcgccac gctttccagc ggttctttca aagcgaaaat gatggtaaaa atgggcaagg
      301 gatacgtccc ggcgaaaaag gaactggatc cggatcagcc caagggaacc atcaatatag
      361 acgctgtttt ctcacccatt aaaaaagtga attacgtcgt ttcctacgcc cgtgtcggtc
      421 agattgctga ttacgacaaa ctgaccttgg aagtttggac ggacggcagc gttaaaccgg
      481 aagacgccat cgcttattcg gcaaaaatac tcaaacagca actggatgtt tttattaatt
      541 ttgaagaagt cgaagaggaa attctcccgg aaaaacagga tgaaccggac aacatcaaca
      601 aaattctatc acggtccgtg gaagatctgg aactgtccgt ccgatcggcc aattgtctga
      661 aaaatgccgg aatcaacacg atcggagaac tggtgaagaa aacagaagcg gaaatgctta
      721 aaaccaagaa tttcggcaga aaatcattga gcgagatcaa agatattctc gcagaatatg
      781 atcttacgtt cggaatggat atcggttcga aaaattagca ttggcggtca atatcaaaaa
      841 tcacgttgta tttattataa aaggaaagag gtaaatcagc aatgtatcac ggtaaagcgg
      901 gcagaaaact gggcaggacg tccagccata aggaagcgat gcttcgcaat atggtgactt
      961 ctgtcattaa acatgaacgc atccgcacga cagatgccaa agcaaaagaa ttgagaaagc
     1021 tggcggagaa aatgatcacc ttgggtaaaa aaggcagtct gcatgcacgc cgtcaggcac
     1081 tggcggtgat ccgggacaag gaaaccgtcg gtaagctttt cggtgaaatg acggaacgct
     1141 atcgcaatcg cgccggcgga tatacacgaa tagttaaagc cggctaccgc tatggcgaca
     1201 atgctccggt ttccattcta gaatttatta atgacgagaa gaaaaaagag aaagcaaaac
     1261 ccaaagccag atccagagcg aaagcgaagg aaaaagcaga gtaagatttt ccatgaataa
     1321 accatcaaaa aggcggcggg ttgtcccccg cctttttttg tggttgtttt ttgataataa
     1381 aagcagtatt gtgaacgccg aattgaaacg ataaccgctg caaagcagat gaggcgggta
     1441 atgggattta acggcggtga gcttaccgtg atgacactgc cgcttgcatt aggcagaccg
     1501 ggatcgttga gtatgatcca gcagacgcag gcgctcaaaa caggaaacga agcagtactc
     1561 gatatcatcg attgtccggt acgggtatat gctttcagat aaactaacaa tggaggtttc
     1621 gttatggcag aaaagaagat cggcgaagta atgaagtttt tctcaaaacc ttcagtggcc
     1681 gcggtaaaga taaccgatgg cgagattgcc gtcggcgaca gcctgaaatt ttccggacac
     1741 accaccgatt ttaccgatgt gattgagtcg atggaggtgg acaatcaatc ggtcccaaaa
     1801 gcggtagccg gtgattttat cggcatcaaa gtttccgatc gtgtccggcc cggcgatgag
     1861 gttttcaaag tcatcccgga ttaatgttgc ccggagatca gcggtttgtt atgaaaatcc
     1921 cgattcaggt gaaaagacac agtggtcttt tgcccgatca ggatttcatc gtcgatgcat
     1981 ggttttctta gaggctagca ggtcatgaaa agcagaattg ccgaagcgat aaaacttaaa
     2041 taccagccgg tagcgctgtt gtggtccgtt gaaaaacctg ctgatgccat gcagtttgcc
     2101 gaagggaaat ggggctgcgt catgtggctg gcggttcacg ccgccaaagg tcgtgcggct
     2161 gttgccgaca ggaaaacatt tggttgtttc ggcggcggcg tcggtctggg gttcggtaat
     2221 cagtacaaaa atttccccgg cggtgaggag ggcttctgtt attttctttc cagcggaaat
     2281 gcccgacgtc cgggcggacc ggaaatggcc gcaaaagtca aaccgttcat gaccagggag
     2341 agccatgata attttctgaa cggcgaacgc tacgttcaaa atcctcagct tgttgaaaaa
     2401 tttatcaggg atcttcctct gacagatatc cccaaaccgt ttgttgtttt tcaaccgctg
     2461 tctgacgtgg atcaggacgg cgaaaagccg cagaccgtca tttttttcgt gaatccggat
     2521 cagttgtccg ctttaaccgt tctggcgaac tacgggcgtg aaggcaatga aaatgtcatc
     2581 atcccttacg ccgccggttg ccaggccatc ggtatttatc cttaccgcga ggctgggttg
     2641 aaaaaccccc gtgccgttgt cggtcttacc gacctttctg cacgggttta tgttcgcaaa
     2701 caactgggcg agtctcatct gatgactttt tccgtcccct tcaatcttta tgacgaaatg
     2761 gagcagaatg tccccggttc ttttctggag cgtcacacct ggcagagtct gttgtctgag
     2821 aaccggtaat tttatttcat tccctgtccg gagagggtat gctagctttg cggagattca
     2881 ggaaggacag atattagccg ggccgtcgac cattccatga aaaattcaag aacatctcaa
     2941 tacctggaca gtttgaacat caataaaatg cggttcggtc tcggggcgat caccagcgtg
     3001 ctgtcgcgtt tgggacatcc acaaaaatca tttaaggcgg tgaccattac cggaaccaac
     3061 ggcaaaggat caaccgctgc gatgatcgcg gccatgctgc acaaagccgg ttattgcgtc
     3121 ggactgtaca catcgccgca tctgattgac gtccgggaac gtattaccgt taacggaagg
     3181 aaaatatccc ggaaagattt cgacagaatc attgtctgcg tcagaaagaa agtcgttcag
     3241 ccgttgacgt actttgagtt tataacggcg gttgcttttg aatatttcaa gacgcgaaaa
     3301 gccgatattg ccgttttaga agtgggcctg ggcggcagac tggatgcgac caatgtttgc
     3361 cgaccgctcg tttcggtcat cacaaacatt gcttttgatc acacggccta tctgggtcgt
     3421 accctgacgt cgatagccgt ggaaaaagcg ggaatcatca agcctcatgg tctgtgcgtc
     3481 accggagtcg cccaaaagaa aatcttagga ttgttgcagg atatctgtct gcagcgcggt
     3541 gcttccttgt accgtttgaa tcacgatttc agactcgcca gacaaaaaaa cggatcgttg
     3601 ctgtatgaag gagtgtacag cgatgtaaaa aatctcaccg tgccgctgcg gggcgaacat
     3661 cagttggcca atgccgccct ggctctggcc gctgttgaaa tgtgcgctcg aaaaggattt
     3721 cccgtctcag aaacggccgt tcgcgccggg cttcaaaatg tccgctggga gggccggctg
     3781 gaggttttac acataagccc ctgggttctg ctggacggag cgcataatcc cgcgggcatc
     3841 ggtgcgttgt gccggtcact gaaaaaagat ttttcctttc gtcatctgat tcttatattc
     3901 ggcgttctgg cggacaagga ttaccgtaaa atgcttcaga aaatagttcc gctggctcgg
     3961 gtgatcattc tggcgccgct caaggcacaa agagccgctt ccgttgccgc ggtcagtcaa
     4021 acgatacgct cattgggccg catgcccgtt gtctctcaaa gtgttcatca ggctctggaa
     4081 ctgggtctgt ctctggccgg gagtcgcgac ctaatctgcg ccaccggatc tttttatctt
     4141 gccggcgaag taaaacagac gttttctaaa ttagtcttct gtgataatga aaaaagcaaa
     4201 aaagtacagg gcaagaaagt ttcatgaaca tcaacacgct gattaacgtc aagccgataa
     4261 aacacaacgg actggcttct ttctccggct gccttgagcc tgtctttacg ttttgtctgg
     4321 ttgcggtgat catcatcctg tggtcatttc ccgcggattg tgcaggcaag caaaaatcct
     4381 cggcgggaaa agcatcacct tccattcaga aaaaaaattt cccggcatcc gttcacacga
     4441 acgaaaagtc ccctgtcagc attgaagccg acgccatgga ttacgaccat gtcagcgatt
     4501 tataccgtgc caggggccag gtgtccgtct tttacagcgg cgcttcctta tacgccgatg
     4561 aagtggaact ggataatcag aatcatgttg cccgggcgca gggcaacacg cttttgaaaa
     4621 tcggagagga tacgctgaag ggtgaacgcc tatggttcaa tattgaagat aaaaccggcg
     4681 ccgcgttcaa tgctgacgct ttctatgcca gaaaccattt tcgtgtccgt ggcaatatca
     4741 tagagaaaac gggagaaaac acctatttta tcgacaatcc ctctgccaca acctgtgacg
     4801 gcagtaaccc ggattgggaa atcgccggca gtgaaatgaa ggtgaccatt gaaggctacg
     4861 gtttgatgaa aaacgcctgt tttaaagtag ggagcctccc cctgctttat tccccgtata
     4921 ttctttttcc ggctaaaacc aagcggcaaa ccggttttct gcttccctat ctggcgtatt
     4981 cccgcgataa agacggaatc gacgtggagc ttcctttttt ctgggccgtt tcgccttgga
     5041 tggatgcgac gttttatcaa cgtttcattg aaaaaagagg atttaaagaa ggggcggaat
     5101 tcagatatga cttcggcaat aaatccttcg gcacgtttta cggcgattat ctggaggata
     5161 ccaagcaggt gacggaaacg gaggatgacg ctctccgacg ggactggcag ggaatgcaca
     5221 aacgctggtc ttatttcctg caccatcaga cgaggctcga ttcgcagttt tatctccgaa
     5281 cggacctgaa aaaggtgtcc gacaagtggt atttcaggga tttttcgtct tataattatt
     5341 atcgggagca ttttaacagc acgtccgaag accgttttcg gaatgtgtct ttcgaaggcg
     5401 acaaatcgct gcgttacctg gattcaacca tccgcgtgta taaaggatgg tccaagtata
     5461 atgtgacggg attaatgcat gcggctgacg attttgccgc tgtcaataat gatcagacgc
     5521 tgcaaagata tccggaaatc atttttgccg gcgtcaagca gcccttgctg catacgcctt
     5581 tttattatga gctggccggc cgttacgatt atttatacaa gaaggagggt cataagggac
     5641 attttgccga tttgtcgcca tctctctcca tgccggtcaa tctttccgac tacgtcagaa
     5701 ttatccccca gtttacgttc aaagaaacgt tctggagccg ggatgatgat gaaaacggct
     5761 taaaagagaa aacggctgac cgctcggttt ataatgcctc ggtgtctttt ggcagccagc
     5821 ttttccgggt attccatctc aacgggaaac actgggaaaa aatcagacac gagatcaaac
     5881 cggaaatcat ctactcctat atgccttcta tttccgcaga ggatgttccc gattattatc
     5941 tgccggcgtt atcgccattc ggaatgcccc ttgcatcgtt gtccagcgat ccgctgaacg
     6001 gacagaatgc cgttgcctgg tcgctgacca acacactgac ggcgagggtc aaagatcagg
     6061 gcgggtcctt aagctatctg gagtttttcc ggctgaaagt gtttcaggcg tacgatatcg
     6121 atgaggcccg gagagatatg gcgcggaatg cacccgagag gcggcctttc tccgacatgg
     6181 gcattgaatt cgatttcagc cctcataaat atttttcttt ccggatgagg gataaatata
     6241 atgtttacag cggatggaag caaaacagtt atgatctgag cctcagagac tggcgcggcg
     6301 acgctctgct tgtagcctac cggaatacgg aaggtctggt ggaggaaatc aacctgggtg
     6361 tgaaggccgt cattaccgat catttctaca gcacctttgt tctgaaacgc gatttgcagg
     6421 acgcccggaa aatagaaaat tctctgggat ttgtttatca cacgcaatgc tggagtctgg
     6481 gatttgatct cagcgatacg gatgacgatg tcagattcct gttcagaata tccctggccg
     6541 gtttcggcaa aaccgcggac aaataaaacg atccggatcc gggccgatgt tcagaatgga
     6601 gccggccgga caatgtattc aatcaagcaa aacaatcttt aaaaaaactt tgtcaggtaa
     6661 ctatggatac atttattctc gtggcgacca gtttcaccat tgccgtttct ctgatgatta
     6721 ccgggaagac gaacaggctt cagtccgcct ttgccgcttt atgtgcggcg gtttttgttt
     6781 cacaggccgc cgtgacgctg gaaaatattt tccattatga atttctcttg aaaataaaat
     6841 atctgggcag cctggccatt gccccggcgg cgctgcggtt tttccatcat ctgaccaagg
     6901 ggcaatcgat tatcctccgg aatttttttt cgctctttgc ggttatcagt ctgtgcggcg
     6961 tcgtttttgt ttttctgccg ttatccgaaa aaacctgttt tcataatgtc atcgcggctt
     7021 atgtgctgtt ctcgttgatg atctgctatt tggccctcct tgggcatgtc ggaacattgc
     7081 cgtcaggtac ggaaaaaaga cgtttcggtt acctgctggt tttctgcccg gtagctttga
     7141 ttctgagtta tttggattta ttaaatttct ggggattcag cttgctgaaa atcaacggca
     7201 tggtatcatc cgcactgctt tattttattt tgctgattat tgcctatccg caacttttgg
     7261 aattacatga ctttttggcc cggtcgctgg ttatcctgct cagcactctt gccggcgccg
     7321 ccatttttta ttttactgtt tcgtttttca acggtgatcc ccccctgacc ggattgctga
     7381 tggcctcttt tctcgtcgtg atttccctgt cgccgatgcg aatgattttc agaaaaatct
     7441 tcggggtttt ctatcccgac agtaaagata tttttacgtc tctttatgaa tttgacgaaa
     7501 agctggagag agaaaaagcc ctgatgctgg aggagatggc ccccgttctc gcccatgaga
     7561 ttcgcaatcc tttggggtcc atcaaaggag cggcgcagta cctgaaaacg gaggcgtcga
     7621 cgggggaaca caaggaactg ttggatgtca ttgtggaagg aacggacaga ttaaataccg
     7681 tggtgagcaa atttctggat tacgcgcgtc cttaccagct tcagctcaaa acacaggata
     7741 ttaacgccat cattcgccgg gccatttccg ttattgccgc aaacaaactg ttggagcaca
     7801 tcaccatcgt tgaagacctg gaagataagc tgccgtttgc ggccgttgac gaacagcagt
     7861 gtctgcaagt cgttctgaat atcgctttaa acgcgatcga agccatgccg cagggaggca
     7921 ccctgacgtt ttgcaccagc agccagaaat ccgaaacggc cgaagactta gcgctcacca
     7981 tccgggacac gggtacggga atcgatcaaa aatacgtcaa ggacattttc aagccgtttt
     8041 tcacaaccaa ggaaaggggt gtcggtctgg gactggccat atgtcagaag atcatcaggg
     8101 aacacaacgg acgcatcagc gtgcagtcga ttgcgtctca gggcaccacc gttgtgattc
     8161 agctgaaaag atccggttga gtcatccgat aatccggctt gattaaaatt tcaaagcata
     8221 ctatttgcaa actatgaaaa agattttaat cgttgatgat gaactgaaca tgcggctggt
     8281 tttgtcggcc atgctgaaga aagaaggttt tgaagtgtcc tccgcttcca acgggcggga
     8341 agccctgcag atccttcaat cgaacaagat ggccttggtc attaccgatt tgaaaatgcc
     8401 gggaatggac ggcatggatc tattgaccca gatttctgca aaccatccgc aaattccggt
     8461 tgtcatgatt accgcgcacg ggacgattgc cactgcggtg gaagcgctga aaaaaggggc
     8521 attcgattat attaccaaac ctttcgacct tgatgacctg aagaatattg tgtctaaagc
     8581 gatcagaacc agcacgttga aagaaagtga attttccatg gcgccttcgg aaatcgatcg
     8641 cctgggcatc atcgggacca gccccgccat catggaaatc tttgaggcca ttcaccgggt
     8701 agcgcaaact accaccacgg tgatgatcac cggcgaaacg gggacgggca aagaactggt
     8761 ggcggacgcc atccattata attcgcaacg caaaaataat cccctgatca agatcaactg
     8821 tgcagccatt gccgaaacgc tgatggagag tgaactgttc ggttatgaaa aaggagccta
     8881 taccggtgcc gccgtgacca agccgggcaa attcgagctt gcccacaaag gaacgctttt
     8941 tcttgatgaa gtggcggaaa ttccgcgcga gatgcaggtt aaactgctgc gtgtccttca
     9001 ggaacaggca tttgaaaggg tgggggggct gcgcaccatc aaggtcgatg tgcgcattat
     9061 tgcggcaacc aatcaacatc ttctgcgtca ggttcagacg ggaaatttcc gcgaagattt
     9121 gtattaccgg ctcaacgttt ttcccatcga tatcccgccg ctgagagaaa gaaaagacga
     9181 cattctgtca ctcgcggatt acttcctcga taaattcaat aaaaaactcg ggatctctgt
     9241 cggcatggac aatgaggtca aagacattct tttacgttat gactggccgg gcaacattcg
     9301 cgaactggaa aacctcatcg agaggatgat gctgctggcc aggggatgcc ggattatgtc
     9361 cggtgacgta cccgatgact tcaaaaaatc ggttgatcat gtcaatacgg ctttatcccc
     9421 cggtataaaa aaaccgttta aggaatatgt gcgcgatcag gtggaaaatg ttgagcggca
     9481 gatgattgcc agggcgcttg atgaatccgg cggcaatatc acgcaggcgg cgaaaaaaat
     9541 gggtttgagc cgcaaggggc ttcagttgaa aatgagcaag tatcatcttc ggaaggaatc
     9601 aaaataaatg atcgttcatc gttcctcaac agaaaaggag gcaaagaatg aattttaaaa
     9661 tcggactggt tgccatcttc gccctgctgg ctttgatctt tctggttcag aacatcgaag
     9721 tgatgacggt acattttctg ttctggcaac tgtcgatgtc gcgcgcgatt ttcctgtttt
     9781 ttatcctgct gaccggtttt atcatcggat ggttcctgca cagcttcgtg tcgtacagcc
     9841 aaaagaagaa aaactgacct gacagaggtc tgagatccca gtcaaagtcg ggtcggcggc
     9901 ggacccgtta gtttttcagc ccgtcataaa tatggagaac cgcctgaacg taagcgtcgc
     9961 tgtggttgta tgcataaacc gctttatacc ggctgcgacg tttatgaacg ttcttgccgg
    10021 agagtccgtg ccggtaaaga tagttttcga cgctgaaaat cgtatcttct atggaaaaag
    10081 gatcaatgtc gtcgttgtct ccattttcgt cgataaagaa agacagcaat gacgacggga
    10141 tgaactgccc ccacccgatg gcaccggcgt acgaactttt gaaataccag ggtgaccggt
    10201 cggtcttttc tgaaaattta tagagcgatg ttatttcacg aacggcaaat ttcttccggt
    10261 cggtgaagat gtattgggag accagggaat taaaagcgta aaattttccg cgataacggc
    10321 gttgagcaaa attggtttcc atgccgatga tggcggcaat caattccttg tgaatcccgt
    10381 gcttcgcctc ggcccgttct aaaatgccgg tataatcctc gatgaatgtt ttgccctttt
    10441 cgatttttgt ttccacaccg aagatggaga agtaccacag gaggctgcgt ttcttttcac
    10501 tgtctgtttt cttttccgcc gattttctga aaaatctgtc gatgttgtgg tgaagccgaa
    10561 acgcataatg gtccatctga tcgtcaaacc attcgcccgg tacaccgttg tccgccacct
    10621 cctgcctcag gtcttcaagg cccgcgttga tggccgagtc cgggagcttc cggacctgta
    10681 tattttccga ggcacaaccg gtaaaaataa aaacgatggc ggcgataatc agtgcggact
    10741 tagcattcat gagacacaac ctgttccttc aaattttgtt tttgtttcac ggtttgattt
    10801 ctactaaaca ataaacggta ttgtcaattt atttcgcggt gcattgttaa atttaaccgg
    10861 gcgatgtctg cccttgtgct catgtgaagg ttggtgtata aagtttccaa ataatagttt
    10921 gtatcggact caagacatgc atctgaccgg ggcgatgttt cattctcgta aatatccgac
    10981 aaaagaccac tatccttttc atttgccgct gttcagggca acaaaacggc tggtgttcga
    11041 tacgccggtg acgctgtttg tgggcgaaaa cggaacggga aaatccacgc tgatagaggc
    11101 gctggctcaa gcctgtcaaa tccatatctg gcgtgacacg cagagaacca gaccggatta
    11161 caatccttat gaagatcagc ttcatcggta tatgtcggtc atctgggcaa acgagccggt
    11221 gcccgggtct tttttgggat ccaagaattt ccagtacttc gctgaaaaac tcgacgaatg
    11281 ggcgtcagcg gatcgcgacc agttgaatta tttcggaggg cgatcgctga ccactcagtc
    11341 gcacggtcag tccctgatgt ctttttttta cagccgttac cggattccgg gcctgtatct
    11401 gctggatgaa ccggaaaccg ctctgtcgcc gcagtcacag attctcctgc tggacattct
    11461 tcagcgcatg agcgccgccg gccatgccca attcatcatc gccacgcatt cgccgctttt
    11521 actggcctgc ccacaggcaa caatttataa tttcgacaga tcgccgttga cgaaaattgt
    11581 gtacgagaaa acaaagcact accggattta caaagatttt ctgctgaaca gaagcaacta
    11641 tctaaagccc aaagcaaaac atcaacgaaa ggacaccaaa tgcttaccaa agaagaaatc
    11701 attgacgaag caaagcgcct gaaatttgcc gatatcggtt tcacgggcgc ggagccgttt
    11761 tgttcgcaga aagactatct gctgacgcac caggaggaat acggctgggc tgaagcgatt
    11821 ggcctggggc tgctggccgg aaccgacccc cgaaatattc ttgccggcgc aaaatcgatt
    11881 atcgttgttc tggaatcata ttttgaagaa tcctttccgc agccgatgga gcgtcatttc
    11941 ggccggtgct atctcgatga cgaccggatt acccaagacg gtttggcgct gaaaattaaa
    12001 gcctttagaa aatatttgaa ggcaaacggt gttgattcca aagtgccgtt tcatctgccg
    12061 caccgtttat cagccgcccg tgccggcttg gggacctttg gcaacaactg cattttttat
    12121 gcacggcgcg tcgcccgcgg cagttctttc gtttttccaa ttgctttgat tgttgattgt
    12181 gaatttacac cggatgaacc atccgtcgca atgggctgtc ccgactggtg ccgcaatgca
    12241 tgcgtggcgg cctgccccac ccgcgcgctc aagggcggag gaaaaattga tccgcgccgc
    12301 tgcatttctt tcctgaccta ttacggctcc ggattgacgc cgcaggacct tcgtgagccc
    12361 atggggatgt acatttacgg ttgtgaccgt tgtcaaaacg tttgcccgcg aaacattccg
    12421 tggatggctg aggaaaagcc ggtcaatcaa cgggtttcag caaaggcgtc ggatttcgga
    12481 ttatcccgtc ttctgcatat ggatgccgta tattttgaaa agaaaatatg gccgcatatg
    12541 ttttatatgt ctccggatga tctctggcgc tggaagatga atgttgctcg cgccatgggc
    12601 aattctctaa atcccgttta tgtcgccgat ctgatccggg cgtttcatga aaattccgat
    12661 gaacgcgtgc gatgcatggc ggcctgggcg ctggggcgta tcggcggtaa tgaggctgtg
    12721 gaggcgcttg ggaggttttt gaaaaacagc gacggcgttg tccgggaaga aattgagcaa
    12781 gctatggtat cggcttccgc aggcgtataa atgaggaacg ttattcttct tcaacggaaa
    12841 tatcggtgat ttcgagattc cgtgtgccgc cgggtgtctt gacgttgatt tcgctgccga
    12901 catttttgcc gatgagggct ttgccgattg gcgacgtcac cgaaatctta ttccggctga
    12961 tatcggattc atacgggcca agcagttgat atttaatttt ttcgccgcta tcaatatcct
    13021 gcaggctgac ataacagccg aaaacaacct tggcggtgtt caatcctttg agactgatga
    13081 cattggacat ggccagctgg ttttccaact cctgcatcct gccgtagaga aacgattgtc
    13141 tttctttggc ggcagaatat tccgcattct ctgaaatgtc gccgtgcgcg cgcgccactt
    13201 cgatgtcacg gacattttca ggaatggaca cgttcttcag tgtttcaagt tcttttttca
    13261 gcttttcaaa acccgctttg gtaatcggca ctttttccat gatctctccg gattgttttg
    13321 ctcttcctga cgtgattatt tttcaacaac aggcaggatg atacgtcgtt tttgtttaat
    13381 ggagaatagt ggcgctgtca agaaacattt aatggccctg tgtaaataat tatgctattt
    13441 atcacacaat gaaaaaagga ttgtattaaa aatgatgctc gataaataca gtagagagat
    13501 aaattatttg cgcgtttcca ttaccgaccg ctgcaatttg cggtgcattt actgccgtcc
    13561 caaggaaggc gttgctctgc acggtcatga ggatatttta cgttatgagg aaatgatccg
    13621 cgttatttcc ctggcggtga aaatgggatt gatcaaggtg cggttaaccg gcggcgaacc
    13681 gcttgtccgg cgcggctttg tcgacttcgt tgcggacctg aataaaattg aaggattgaa
    13741 agatatcagt cttacgacca acgggatatt actcgaacag tttgcacagg gnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgaaagata tcagtcttac gaccaacggg
    13921 atattactcg aacagtttgc acagggaatt tttgacgccg gcatccgccg catcaatgtc
    13981 agtcttgatt cgctcaaccc ggacaaatac ggccaaatta ccaggggcgg acgcttggaa
    14041 gatgttttgc ggggaattgc ccgcgcggaa gaaatcggtt tttcgcccat caaaatcaac
    14101 acggtggcca tcaaaggatt caacgatgat gaagcgctgg attttgcaag gttggctcag
    14161 gagcagccat tccaggttcg attcatcgaa ctgatgccgg tcggaaaaac aacccttgat
    14221 tacggcgaag attatatgcc cggatcgcag ttaatcaaaa tcatcaggca acgctatgcg
    14281 ttggaaccgc tcaaaaacaa aaaaaacaga tcggacggtc cggcaaaaat atttaagatt
    14341 aaaggtggac ggggagaaat cggttttatc aatccggtga gcgaccattt ctgttcaacc
    14401 tgcaatcgca tgcgtctgac gtcggacgga aaactcagag cctgcctgct caatgaagcg
    14461 gaagtggatc tgaaaaaagc cctgcgggaa aattgtagtg atgccgaact ggaaaagctg
    14521 atctgggatg ccgttctgtt gaaaccccgg cagcacgaca ttgtctgcac ggaaaacagt
    14581 ctgcgaaaat gttcccggaa tatgtcggaa atcggcggtt gaaaaatatt ttccaaaaat
    14641 atatatttaa aaaataatgg ctttatttcc ggcggcaggg ggctgtcgga ccgcatctgg
    14701 aaaagacggc aatacatcgt tacatcgttg tgaaaatctg ggtaggatca agaccatgtt
    14761 ttttgatcaa aagaaaaccg gatttccggc tgaaagatta aaaacaccga gggacgatta
    14821 catcttttcg aggcagaaag agctcgttct ggatctgatt actcccctgg ccggtgaacg
    14881 tcttctggat atcggttgcg gcaccggcaa ccatctggat atgttccgcg gaaaatggtg
    14941 ttctctgacg ggccttgatt cttccggcgc aaatctggaa atcgcccgtc aaaaacacag
    15001 tgaacgtgcc gaactgattc tggccgatcc tgaagacatt cccttctccg acaatgagtt
    15061 tgacattgtc acgatgattc attgtctgga aaccgccgtg catccgcaaa aaatcattga
    15121 cgaggccgtc cgggtctgcc gtggccgtgt ttttatcggt tgtgtgaaca attactcttt
    15181 tgtcggaacc agacagcgcc tgaaagaaat gttcggtttt tctttttctc aaaaaatccg
    15241 ttttttcagt ttacctgaaa tcagaaaaat ggcggaggcc tcggccggca ataacgccat
    15301 gaaatgggga agcgttattt attttcccgc catggtttac gatttttttg aagaacttga
    15361 ggagatgctg cccttccgga aaaacccctt cggggcgttt gtcggcttgg tttgtcccgt
    15421 aacgtatacg taccggacgg cacagaatcc ggtgatgaat tcattcaata tcaaggcgga
    15481 aacacgcaaa acggctcctg aagctgtgag gaacatgttg aaagaggcag accgatgatc
    15541 aaagaagcca tgctctacga aaaacttcaa gacggaaacg tccgatgcaa tctttgcgcg
    15601 caccgatgca cgatccatcc cgagcgtcag ggtgtctgcg gtgtacgggg caatcgggac
    15661 ggtgtgctgt attcgatggc ttacgggaca ttgattgctc aaaatgtcga tcccatcgaa
    15721 aaaaaacctt tctttcatgt ataccctgcc tcacggtctt actccattgc cacgatgggc
    15781 tgcaattttt tctgtgactt ttgtcagaat cacgatattt cacaagcgcc ccgaaaaaca
    15841 aaaatgattt acggcgagga ttccatgccg tcagacatcg tggagcgcgc gaaagaaagc
    15901 ggcgctaaaa cgatcgctta tacctacacc gaaccgacgg tgttttttga actggcgtac
    15961 gataccgcaa aaatcgcagc cgcaagcggc ctgaagaatg tttttgttac caacggtttc
    16021 atgacacctg aagccatcga aatcatcgcg ccgtatttat ccgcggccaa tgtggatttg
    16081 aagtcttttc gcgacgagtt ttataaaaaa cgatgcgggg cacgtctggt tcccgttctg
    16141 gacagtttga aaaaaatgaa agaaagaggg atttgggtgg aaatcaccac gctgcttatt
    16201 ccgtctctaa atgacagcga agaagaaatg agagatatcg cacaatttat tgccggactg
    16261 ggaagggaaa cgccctggca tatcagccgc ttccatccac agtacaaaat gcagcacctg
    16321 ccggtaacgc ctcttgaatc ccttcatcgc gcagggcaga tcggcaggga ggccggatta
    16381 aaatatgtat acagcggcaa cgttcccggc gatgatgccg aaaacacgcg ctgttttcac
    16441 tgcggcaaac tgctgattga acgctacgat tttaaaatta tcggcaccaa tctgcaaaaa
    16501 aacaaatgtt cgcactgtgg aacacaactg gaaggaatat tttaaacgac accttttttc
    16561 atgttttgcc ctcaacgatt tttttgcagc tcttaatgtg ttgaaaccag ctcatccaat
    16621 caacagatgg ccagccctcc ggtgcccgat ttcccacgga tgatgaagag agggtatcgg
    16681 ctccgctgat cggttcttgc gcgctttttt catgagatgg tctttattgt actgtatatt
    16741 taaatcacgg gtcgcaggag tgttgtttga atcgggctgt ttatgcaaag attgacaatg
    16801 atccgcattt ccgtatatat tacgtgaaaa tatcaaaaat atgcttggag gaaaacccat
    16861 gaccgaagag atcaagcagg agcagccggc cactgatcct catgaacaag agaatcagcc
    16921 catcactctt aaagagaaga tagcggcggc tgttttgatc gcttatgtga tttccctgat
    16981 ctggctggca gggagcaaac actggtttgg cctctggtaa agcttgcaat tcccggcgga
    17041 aaaaatctta atttaagctt tgggcaaaaa acagttgagc ccggaacgta ttttcttttg
    17101 gatcatcgtc ttatcagtaa ggtgatctga gaatgatcaa aatgatacgc acggccggct
    17161 gtttccaccg gttactcttc atattttacc gatttggttt tattcctccg gcaaagggga
    17221 caaaatcgtt acggacattc tcaacgttta aacatttttt acgaacgtct ttcagccata
    17281 tcaaacaagg aggcacgaat gcggtttgag catctctttt caccgtttac cataaacgga
    17341 atgactttga aaaacagggc ggtgatgccg ccgatggcaa ccggttacgg taatgccgac
    17401 aacacggtca gtgaacgttt gctggcttat ctggagcgca gggcacaggg cggaacaggg
    17461 ttgattataa ccgaggtctg cgcggttgat ccgcgcggaa aagggtttcc caatgagatt
    17521 ggtgtctgga gcgatgattt catcggcgcg ctgtcacgga ttccggccgc gctgcaccgt
    17581 cacggcgcca aggccgcgct gcaattgcat catgcgggcc gtgaaacgtt tgaagcggtg
    17641 ctcggcaaaa aacccgaggc tccttcggcg atacccagtg ccgtattggg gcagccgtgt
    17701 gaagcgatgt ctctggagcg tattgccgaa atagtccgtg cctttgctca ggcggccgga
    17761 cgcgcccgtc aggccggatt tgattcggtg gaacttcacg gtgcgcacgg ctatctgctg
    17821 aatcagtttc tttcaccgtt ctccaacaac cgtgaagatg agtacggcgg aagcgaggaa
    17881 aaccgcatgc gctttattct ggaagtcatc gcggcggcgc gcaaagcggt gggagacgat
    17941 ttccccctct ggattcgcat atcggcggat gaaatggttc gcggtggata tgatttttct
    18001 ctgatgcgca gattagcccc ccggatggtt gctgcgggtg tggatgccat ccattgttcc
    18061 gtgggggttt attccacgcc gggaggtctg tccatcgcct caatggatac cgaacccgga
    18121 ttcaatctgt tccgggcccg tgcgcttaaa gaggtggtga gcgttccgat catcggcgtg
    18181 ggtcggatca atgatccggc aatggccgat caggccattg cccggggcga tgccgacctg
    18241 atcagtttcg gacgtcagca tcttacggat cccgatttta tcgaaaaagc ccgcagggga
    18301 tctctggagg atatccgccg ctgcacagcc tgtaatcagg ggtgtatcga gcgtttgagc
    18361 ttcgagatga aatccaccac gtgcagtttt aatcctgaat gcggacgcga atacaaagga
    18421 cctgttttgc cggccgggac gccgaaaaaa gtatgggtga tcggcgctgg accggcggga
    18481 ctttccgccg ccctggtgct ggctgcacgg ggacatcagg tggaggtttt tgagaaagaa
    18541 tcccagccgg gcgggcaact gctgtcggcc agccgtccgc cgcacaaagc cgtttttatg
    18601 gattgggtaa actgggccct gcgccgtctg aagcaatggt ccgtgccgtt gcactgctct
    18661 catccggtca gcgccgatga catcaaaaag gcagcgcccg atgccgtgat cgtagcgtcg
    18721 ggcgcttacc cggttaccgc ttccattccc ggtctgaaat ccgaactcgt ggctgatgcg
    18781 cgggatgtgc ttaccggaaa tatcaaactt gccggtccgg ctgttgttct gggagccgga
    18841 tatgtcggca tggaaaccgc tgattacctg ctggccaacg gcattgccgt aacggtgctt
    18901 gaaatgcaga cccttccgcc ggtgaacagg ttttccgcgc acggatactg gctgcacaaa
    18961 cgcatcaaag acagcggggg aaaaataata ttcggcgcca gagtcctcgg gattgaaaac
    19021 aacatcgtgc gatatgttca ggcggacaaa ccaagtgagg aaaaggcatc cctgatcgtg
    19081 accgccatgg gcgcgaaatc ggaaaatact ctggaggatg tgcttaacgc gttggccgtt
    19141 ccttaccggg ttgtaggcga tgcccgcagt ccgcgccgga ttttggaagc cattcatgaa
    19201 ggctacaagg ccggggaaga aatctgacag tgatcgtcct tcggaatcgg caagcctttc
    19261 atcaaaaagc acaagcggat cccgggagga tttacattgt gcttgccata ttcccatttt
    19321 tcttttaaaa taagttcaca gccgagaaaa ataaacagac tttatcagga ggtgtggatt
    19381 atgcagagag tgagcgacat tttaaagagc aagtcgaagc agatgtggac aattaatccg
    19441 aacgatacag tatttgaagc ccttaaactc atggcagaaa aagaaatcgg ggcattggtg
    19501 gtcgtggata agaaaggtaa agttgccggc ataatcacag aacgcgatta cgcgagaaaa
    19561 atcgtcctga aaggaaaaac atctcccaaa acactggtca aagaaatcat gacgcctttt
    19621 aataagatgt ataccgtgaa gccggacacc tcgatggagg gttgcatggt attaatgacg
    19681 ggcaagcata ttcggcatgt tccggttttt gaaggaagca aatttctggg gatagtttct
    19741 attggtgatg ttgtaaaatc gacgatttct gaaaaagatg tactgattaa ccagttgagt
    19801 aactacattg cggggaagta ttaattgcta aatatttcaa taatttaagt ttatgtgttg
    19861 ctttcagggc aggggaacat cccctgcctt ttcttgcttt ttcatggttt ttctctttaa
    19921 tttcgatgaa gtacggaatt tcattaaaaa gctaaaaaaa tcaaagcact acatattgtg
    19981 gtatgaatat attaaaacca cattttattg tattttcact ttacaaaggt atattctttt
    20041 gctaaaattt tgcaaatttt gaacaattaa ataaagctgc ttgtttatga atctgaaaaa
    20101 tttggaaata tcggggttta agtccttcag ggacagggtc gtgctggatt tctccaacgg
    20161 tgttacgggt attgtcggtc ccaacggttg cggcaaatct aatatcgtcg atgccattcg
    20221 ctgggtgatg ggtgagcaga gagtcaaggc actgcgaggc aaaaaaatgg atgatgtggt
    20281 cttcaacggc agccaggatt cggcaccggt cagcatggct gaagtgacga tgacgttgta
    20341 tgccaacggc cagagttttc ccggtgctta ttccgattta aacgaagtct ccatctcccg
    20401 cagagttgtt ctggacggcg atagcgaata ttttattaat aaagttccct gccgtcttct
    20461 cgacatcaag gaattcttca tggggaccgg tgtcggtgcc cggacatatt cgctggttga
    20521 acagggccat gtcaccaatc tggttgaggc taagcctgaa gacagaaggt tgtacatcga
    20581 agacgccgcc ggcgtttcca aatataaaag ccgcaaggaa gcggccgtcc gtaagatgga
    20641 cctgacgaaa cagaacattc tgcggctcaa cgatattatc aaggaagtca aaacgcagct
    20701 taacaccatt tcccgtcagg cgaagaaagc ggaacagtac aaaaaaatca agcaggaaat
    20761 ccgagaagcg gaaataacgg ttgccctgca aacctatgtc gactttatgg ccagggagtc
    20821 ggatctgcag caacagcgca cggaattaca gcagcaggaa gctctcatcc gggcggattt
    20881 ccaagcgaaa gaatcgatgc tggatgaaat gaaagtgaaa cttctggaga atgaggaact
    20941 gacggcgaaa gttcagcagg aactttacga aatcaaaaac accatcagta tcaaggaaaa
    21001 aagtattgag tttgcccaac gtcaggtgac cgatatgtcc ggacgccggg agaaagattt
    21061 gacggaaatt caggtgctgc agacgagaaa agccgattta gccgctgaaa tcgaaaatct
    21121 gcggaaaaat tcggcagaag cggaaaataa aatcgtcgct ttgaaagccg aacacgaatc
    21181 gcttcagcaa aaagtccagg aactcaccga agccgacaag gcattgagca gtcagctgga
    21241 agaaaaaaag attttgttta tcgatgtcgt gacggaaaaa gccaaacttc ataatatgat
    21301 ttccagcctc agcaaaaata tcgaggatct gaagaaacga gaggaaaagg aaacccgcga
    21361 actggaagaa gatcagaaga tgatggccga cgtatgcgcc aggctggagg cggccgacga
    21421 agccttgaaa aaagagaaag cggacattgt cggactggaa gctaaaatgg cggaagcggc
    21481 aaccgaactg gatcgattca gatcggattt acagtttaac gaggaagata ttgcaagact
    21541 cagggaagac accagcgtca aatccacccg actgatttct ctgcgggagt ttcaggagtc
    21601 atccaaatgg agtaacgagg gcgttaaaac gattatcgag aataaagagc acagtgataa
    21661 attttacggc gtgattgccg atcatatcag tgttccccgt gaatatgagg ctgccgtgga
    21721 agccgtcctg ggtgaaaaac tgcagtacgt ggtagtgcaa agtcaggaag acggtgtgcg
    21781 cgctatcgat tacttgaaga attatcagtt gggccggggc agttttattt ccgtcgattt
    21841 gagaacccat gatgccagga cttacagcga agcgcacctc aatgaagcga aacccctgct
    21901 ggacaaggta acagtccgcg atgattttaa acccattatc gactgcctgc tgggtgacgt
    21961 tctgctgatt cccacgattg aaaacggcat ctctctctgg aagaaaaacg gtttccgggg
    22021 gacgtttgtc acgccggagg gcgatatcat cagcccgcac ggtgtgttaa ccggcggcag
    22081 cggttcggcg gtggaaaaaa gcctgctggc gacaaaaaga gaaatcggcg aactggaggc
    22141 agagattgca gccttatctc tcgatctgga aaccaaggtt ggccggagaa ctaaaatctt
    22201 agcgaccatt tcccgctggg aagaagaaag ctctcaaatg aagacggaaa ttcatgcgct
    22261 ggaaattacc gtcaacggca gaaaaaagga tcgcgaacgt tttgacgacg atgtcaacag
    22321 actcagacag agaatcgccg ttctggagtt caacagacaa accctgaata cagaaaagac
    22381 ggaagcggaa gaaaagctcc gaaggttcaa tgaggatgtt ttgcaaaagg acggtgaaga
    22441 aaaacagatc aacgacatca tcgccgcgtt aaacgttcaa aaagaacagt cccgcgtcca
    22501 gattgacgaa caggaacgtt atgtaaccgc cgggaaaata gaactggcct ctctggagga
    22561 gaagaaagaa ggggatgtac ggacgatatc caggctgcag gatcatattg attccatgga
    22621 acaggaacag aggaccaaag aagaggacat cgtgttctgt gaaaagcaga tcgccgaatt
    22681 gacgcaaggc attgagaacg atcaggtttt gttaaaagaa atatacagcg cgtttgcggc
    22741 acaggaagca ttgctggccg agaaaaaagc cgagcaaaac cgtgaagatg cacaactaaa
    22801 gatcctggaa ggtgaaatca gggaaatcaa gaagaatctg gatgaattac gccagcaggt
    22861 caatgcaatg gaatttcaaa gccgggaaat aaccctaaac agagaaaacc tcagaaaaag
    22921 cgtggccgaa aaacatgatg ttgatctggc cggcatggcc gcggattttg aaaaggtcac
    22981 ggaagaaaaa ctggtcgaga tcgttgatgc tctggaaaaa aacaaacaga tgctcgagac
    23041 attcggcgag gtgaatctcc ttgccttgaa tgagtatgag gaactcgacc agcgctacaa
    23101 ttttctgtcg acacagattg ccgatctgaa tgcatccatc aatgttttgc agcggaccat
    23161 tgcccggatt aataaaattt cccgcgccag atttgcggaa acctttgaag ccgtcaacgc
    23221 gtgcttcaaa gaagtcttcg cgcagatttt tcccggcgga cgcggagaac ttcttttgac
    23281 ggacgaaaat gatatgctgg aaacaggcgt tgatattgat attcaggtac caggcaaacg
    23341 caagcagaat gtgagtctgc tttccggcgg tgaaaaatca ctggtggcgg ttgctcttat
    23401 ttttgccatt ttgaaatatc gtccctcgcc gtttctggtg ctggatgaag tcgatgccgc
    23461 cctggatgat gccaatacaa atctgttcaa tcgtctgatc aaggatgtcg ctgggaaatc
    23521 ccaggttgtc atgattacgc ataacaagag cacgatggaa gttgccgaca cgctcttcgg
    23581 cgtgaccatg cagaaacagg ggatatccac tctggtttcc gtcaatctca attaatccgt
    23641 ctctttactt cttacaaaaa agcggttatg aatcaattgt ggcaggatga aaggtgcttc
    23701 tatggctaaa tcgagtcttt ttgaaaaatt gaaagcgggg ctggtcaaga ccagaaccgc
    23761 cctgaccaag agtctggata atctggtgtt cggcaagagg gttctggacc aggaactttt
    23821 cgaagatctg gaggaacttc tgatttcagc ggatatgggg ccgcagttta cctacgatct
    23881 gattgatgac gtcaaacagc gcgtcagccg caatgaattg aataacgctc aggagatcaa
    23941 aaaagtttta caggaacgca tgacggccat tctgcaaaaa atggaaaagc ccctgatcat
    24001 accccgcggg aaaccgtttg tgattatgac catcggcgtt aacggcagcg gcaagaccac
    24061 gaccatcgga aagctggcca atatcctgaa aagtgaaggc catgaaattt tgctggtggc
    24121 tgccgataca tttcgggctg ccgccgtcga acaactcgag gtctggggag cacgcgtggg
    24181 cgctcccgtt attaaacaca aaacaaacgc cgacccggca gcggtcgttt ttgacgcgat
    24241 gtcgaaagtc aaggccggtt tcaacggggt ggtgattgtg gataccgcgg ggcggctgca
    24301 cacccgtgtc aatctgatgg aagaactgaa aaaagtgaaa agaatcatca acaaggaatt
    24361 gccggatgtg ccgccggaga ttctgctgat tctggatgcg accaccggcc agaatgccgt
    24421 ggctcaggcc aaaacgttta aagaagaaat cggcgcgaca ggcatcgtgc tgacaaaact
    24481 ggacggcacc tccaaaggcg gagtcgttgt gcgcattgcc agtgaacttg cattgcccgt
    24541 ctggtatatc ggtgttggtg aaggcgtgga cgatctgcgc gtttttgaaa gcagcaattt
    24601 taccaaagca ctgctggatt aagagccgag gcaacgggag cagatccata tggggaaaat
    24661 ctatgccatc ggcgatattc acggttgtct gacaaagctt aaaacgctga tatcggatat
    24721 tgacatcgat ttcaaaaaag acaccctggt gtttatcggc gattacgtcg accgcggtga
    24781 attcagcagg gacgttgtcg attatgtttc cggcctgcag gattcgcatg aaaacatagt
    24841 ttgtttgctc ggcaatcacg aacaaatgtt tatgaattat ctggagggcg tggatgaaga
    24901 gatttatctg agaaacggcg gcatgcagac cctgcgttcc tatgaaatat ttttgtccga
    24961 tgatatcaag gaaagaaaac gtaaaatgtc cgcaaggcac cggaaattct ttgaaaccct
    25021 tctgccgtat tatgaaaccg aaaaatatat ttttgttcat gccggtctca gaccagggat
    25081 tccggtcgcg gaacagtcgc aaaacgatct attatggatc cggtacgagt ttatcaatac
    25141 agaaaaagac tttgacaaga cggttgtgtt cggtcacacg ccgttaatga cccccctgat
    25201 tgacaaaaac aagatcggca ttgataccgg cgcagtttac ggcgggaaac tgacctgtat
    25261 cgaatttccg ggcttaaaaa tttatcaggc atagacatga agatcattga atgcgtaccg
    25321 aacttcagtg aaggacgcga tgacaaaaaa atcttatcca ttgtttctgt ttttaaaatg
    25381 tttcccggcg tccgtttagc cgatttcagc ggggacgccg atcataacag aagtgttttt
    25441 acttttttgg gaaaacccga tgatgtgctg gacgcgacac tggccgcctg tggaaaggcc
    25501 ctggaattaa tcgatatgcg gcaacagtcg ggcgttcacc cgcggctggg tgccgtcgat
    25561 gtcgttcctt ttattccgct gggccgggca aaaatgaaag atgccgttga actggctcat
    25621 gcgttcggca tgcggttttc ccagcggttc ggcgtgccgg tgtattttta cggttacgct
    25681 gcgcgcaacg gtcggaatgt tgaacttccc gatgtccgcc gcggcggtta tgaaagactg
    25741 gctgataaaa tgtcttctac ggaaagcctt cccgatgcgg gagaaaatca attcaacgcg
    25801 cggtccggcg ccaccgctgt aggggcgcgg gatttattgg ttgcctacaa tatcaatctg
    25861 gtctctgacg atctgcttct ggcgcggcat atcgcctcgc gcatcagaga gaagggcggt
    25921 ggattgaaga atgtgcgggc gatcggagtg atgctgaaaa gacgaagact ggcgcaggtc
    25981 tcggtcaatc tgaccgattg taaggaaaca cccttgaaaa ccgtctttga ccgggtgaaa
    26041 gctctcgcgg ctgaaggcgg tgcgcagatt ttggaatcgg agttaatcgg attggcgccg
    26101 aaatgcgctt ttgccggtgc gacgccagaa tatctcagac tgaaagattt tgatgagaat
    26161 cgtctgctgg aaacacacat caggcgcctg tcggaatagg ttactctttt tttttcgcct
    26221 cctttttttt cgctttggca atcgctttga ggagtttttt gtcgggaagg tcaccggatg
    26281 atacaggctg cggtgccgga gtctgcaagg gagtttccgg ctttattgtt ttgttttcct
    26341 gttggggccg gcctgcacgg atttttttgg cgatggcttc agccgccatt ctgccgaatt
    26401 gtccgataaa tccggcaatc agcacaagca caacccattt cagaacagac cagttgctga
    26461 tatatgccag taaatcattc atgtttaaaa acctagctta gttgcaaaag ttcgccgggc
    26521 gtatcgaaca ggtaatccgg ttcggcagca gccattcttt ctctgctgtg ccatccccag
    26581 gtcactccca cggtttttac cccggcctgc tttccttcct tgatatcgcc ggtcgtatcg
    26641 ccaatataat atattttatt gggttccaca tgatattttt ccgcggcata aagaatcttt
    26701 tcttttttgc tgaacatgaa atcggaaccg agtatgtccc ggaaataatc attgaaatga
    26761 aataaatcaa gggcctcttt gatggaggca taatcattgg aggaaacaac aatcaggcag
    26821 tggttcttgt gcagcgtttc gataaccggc gcaacatccg gaaacggttt catgtccgtg
    26881 atccggacct gcgcgagaat atcctcggcg gctttcataa acacattcag atcaatgccc
    26941 ttgtttttca gcgaggtata gaaattgtct tcaaagagtt ccagaaattc ctcccggctt
    27001 tgtacaacag gttgtttaat tttttgaagg ttgtccttga ccgttttttc ataaacgtca
    27061 agcgagtcga caagcacgcc gtcaaaatca aaaaggaata atttcatgat cccctttctc
    27121 ctttatacag acgggcaatg gatgcgcgga aaggttttct taaaattcct ttctctgtga
    27181 tcatcgccgt gatcagccgg gcgggcgtga tgtcaaatgc cggattataa acgatcgttc
    27241 ccctgggagc gctgcgggtt ccttgaagat gtgccacctc atcgcttttc ctttcttcga
    27301 tgggaatcga gtctcctgtt tttaatgaaa cgtcaatcgt ggaaagcggc gccgcaatgt
    27361 agagaggaac gccgtgagcc tgcgccagga ccgccagaga ataggtgccg attttattgg
    27421 ccgtgtcgcc gttggccgca attctgtctg ccccgacgat aatcttgtct atttttttct
    27481 gctgcatgag aaatcccgcc atattatcgg tgattagtgt gtaagggatt ttttcttttt
    27541 tgagttccca ggcggtaagc cgtgcgccct gcaggacagg tctggtctcg tccacgtaaa
    27601 cgtgcagttt tttgccttgt ttacgggctg cacgaatgac gcccagcgcc gttccgtatc
    27661 cggctgtcgc cagcgcgccg gcattgcagt gcgtcagaat attgtcgccg tcggcaatta
    27721 tccggcttcc gtttttcccc atttgttgat tgatggctat atcttcggag cagatatgct
    27781 tggctttttt gattagttct ttgactatat ctcgttggga tagttgactt gaatgactga
    27841 caattttttt catgcgctcc agcgcccaga aaagattgcg tgccgtgggg cgtgcctggg
    27901 caatctcatc acaaatggcg taaaactttt tccggaattt atccggcgat aaagaggcaa
    27961 gatgaatcgc tcccagagcg gcgcccatcg cagcggcaac gccgatggcg ggagctccgc
    28021 gcacagtcaa atctttaata caggcaatca ctgctttgta tgatttgcag actatatatt
    28081 tctcggcaag cggcagagct ttctggtcga ttaaaacgac cgcattgttt ttccagaaaa
    28141 ttgttttaat catctttctt atcaagaatt tctttcagga tttcattgac aagctgcgga
    28201 ttggctttgc ccttggtctc tttcataacc tgaccgacaa aaaaaccaaa aagtttttcc
    28261 ctgcctccac cgcgataatc atcacgctgc tgcggatatt taccgacggt tatgctggct
    28321 gttttaacca gagcatctct atcggtaatc tgcaccaggc ccttttcctt gatgatattc
    28381 tgcggcgatt tgccggtctt atacatatcc tccacaactt ccttggccat ctttccgctg
    28441 atggcgcctt cttcaatcaa tcgaatcata tcggccagcg attcggcctt gatgggacat
    28501 tcgcggacag agcgtttatc ttcattgaga aaacgcataa cgtctcccat gagccagttg
    28561 ctggcgattt tgggtttgtt gcagagtttg accacctttt cgtaataaag agccagcgcc
    28621 ttgtcggcgg tcagcacgcc ggcatcgtaa gccggtattt gataatcctt gatgaatctt
    28681 tcccgtttgg ccagcggcag ttcgggaagc tcttttctga ttttttccac ccacgcttca
    28741 tcaaccagga tggggacaag atcgggatcg gggaagtaac ggtaatcgtg cgcttcttcc
    28801 ttgctgcgca tcgaattggt ggcgccctgg gcgtcatccc aaagccgggt ttcctgaatg
    28861 actgtgccgc cgttttccac caggtattgc tggcgcttga tttcgtattc cagcgcgcgc
    28921 tgcacattgc ggaaggaatt catgtttttc aattcggtcc gggtgccgaa tgccttctgg
    28981 cctttgggac gaatcgatac gttggcatca cagcggaagg aaccttcttc catgttgccg
    29041 tcgcaaattt ccagatacac tagaatttcg tgcaggcgtt tcagataatc ggctgcttcc
    29101 tccggcgaac gcatatccgg ttcgctgaca atttcaatca gcggaacgcc ggtacggttt
    29161 aaatcgacgt aactgaccgg gttgtgctcg tcatgcagca gtttgccggc gtcttcttcc
    29221 atgtgaatcc gggtgatgcc gatttttttc tgctcgccat tcgtctccag catgacatag
    29281 ccgtgttcgg ccagcggata ggcatactgg gatatctggt accccttggg cagatcgggg
    29341 tagaagtaat tcttgcgcgc aaattcgcat gatttattga tttcgcagtt ggttgccagt
    29401 gccatcttca gggcgtattc cacgactttt ttattcagta ccggcaaaac gccgggcatg
    29461 cccagacaaa ccgggcaggt gtggctgttg ggtgcagcgc cgaatttggt ggagcagtta
    29521 cagaaaattt tcgtgtccgt taacatttgg gcgtgaactt ccagcccgat gactgtttca
    29581 aaatccatta taatgcaggt ctcctctttt caatcttggc gtttttctcg taagcagagg
    29641 cgatttgaat gagtttgcct tcttcaaaat ggctcgccag aaactgcact ccgatcggca
    29701 gaccgctcga actaaatccg cagggaaccg agattcccgg tattcccgcc aggttggccg
    29761 agatggtgaa aatatcggaa agatacattt gcagcggatc atctgttttt tcgccgattt
    29821 taaaagccgg tgtcggggat gtaggcgtca taatcacgtc gcaaatttta aacgctttgt
    29881 cgaaatcctt cttgatcaga gccctcacct gcgaagcttt tttgtaatag gcgtcataat
    29941 atcccgcgga caaggcatag gtgccgatca taattcttct ttttacttcc gcgccgaaac
    30001 cctgcttgcg ggattcttta tacatcatca aaagatcccg gacatcttgt gcccgaagcc
    30061 cgtaccggac gccgtcatag cgcgccagat tggaactggc ttccgccgga gcaataatat
    30121 aataggttgc cacacagtat tgtgtatgag gcagtgaaat atccacgcac tggccaccgc
    30181 tctgttcaat gacggtaatc gctttcttaa tggcggcatg gactcccgga tcaatgccgt
    30241 cgataaaata ttccttcggg atgccgattt tccagccttt gatgtcgcgc ccgacaaatt
    30301 gccgataatc gggaacttcc tcgttcacgg aagtggattc tttcggatca tacccggcca
    30361 gcacattcat catgatggcg cagtcctcga catctttggt aaacggtccg atctgatcaa
    30421 gcgatgaggc aaaagcaatc agaccgaagc gcgacacccg tccgtaggtc ggtttcatgc
    30481 cgacagtacc gcaaagcgcc gccggttgac ggatggagcc gccggtatcg gaaccgatgg
    30541 aggcgatgca ttcatcggcg gcaaccgcgg cggcggaccc gccgctggag cccccgggaa
    30601 tccgttccag atcccatgga ttgcgtgtgg caccgaaata ggaagtttcc gtggacgaac
    30661 ccattgcgaa ttcatccata ttggccttgc cgacaaatat cgctccagcc ttgcgcagtc
    30721 tttccacgac cgttgcatca tacggcggaa taaaattatg cagaatgcgc gatccgcagg
    30781 tggtgggaat gcctttggtg cagacaatgt ctttcagagc gataggaatg ccggttaaag
    30841 ctcttatgtc accttgttta atgtcttcat ccgcttttga cgcggcctga aaagcctctt
    30901 cctgcatcag acgaatatag gcatgaacct tttcgtcgac agcattgatt ctggcaaaaa
    30961 catcatgagt aatttgcgtg gccgatgttt cgccggtttt gattcttttc tgcagttcat
    31021 gaatggttaa ctgattcaac ttcataaaat tcctttatat ttaattaggg cctggaatag
    31081 tcaacgttgt gatttcggac gagattttaa tcgattactt tggggacgcg gaagaactca
    31141 cctcgggtgt ccggcgcatt ggccaatgcc tcccgggtgc cgatggagtt tgatatctta
    31201 tcttcgcgaa acgcattggt tatgctgatt gcatggctta tcggtttcac atccccggta
    31261 tccagttcgt tcaacttatc aatatagagc agaatgtcgt ttaactgagc cgtaaatttc
    31321 tctttttctg cgtccgttat ttccagacgc gccaaatgag ccacatactc aacttcgcgg
    31381 gaacttattt tcaaaaaaca tcatctcctt taaaaaactt tccagtaggg atttattctg
    31441 gtgatcacct tatcgatcac ggattccgtt gttctttcat ccacgtcttt ctccatcaga
    31501 gacaagcgtt cttccgagaa atgggtttca tccagcattt cgttcaagac ggcttttacc
    31561 gacttgacca gtccgctcag atgatatcct ccttccagga cggctacaaa acgtccctga
    31621 cagcaggtgt cggcaatatt gagaaggata cgcgtcatag cggcgtagcc ctccggcgtg
    31681 acctgcattg cgcccagcgg atcctgataa tacgtatcaa aaccggcgga cagcaggatc
    31741 aattcaggtt tgaattccag cgccacgggt tcaagaatct tccggaatat cttgacaaag
    31801 accgcatcgc cgacagacat ggtcaggggg acattgatcg tataatattc gccctggccc
    31861 cggccggttt ccgaaatgct tcccgttccc ggataatagg gatattgatg cgtggaaaaa
    31921 tataaaacgc gctgatcccc gtaaaaactg tgctgggtgc cgttaccgtg atgcagatcc
    31981 cagtcgacaa tcagaattcg ctttaaattg tgtttttgga tggcatgcat ggccccgatg
    32041 gcgatattat tgaaaacgca gaagccggcg gccgtatctt tttccgcatg gtgtcccggc
    32101 ggtctgacaa aagcaaaagc gttatccacg tcgcctttca tgacgctgtc aatggcattg
    32161 cagacaccgc cggcggctag ctttgcggcg tcgtaagttt ccggagatgt cgatgtgtcg
    32221 ggatcgagaa aaacgctgcg cttgcccgcg gtgctggcga taaagtccac ataagagcga
    32281 gcatgaatcc tttcaatttc ttcgtgagtg gcttcacgcg gttcgatcgg cgtgaatttc
    32341 caggacatca gcgggttttc cagcatttcg taaatgacgg cgagcctctc cgggctttcg
    32401 ggatgagcaa aacccatcga atgctgcatg tattttccat ctttgacgac gcctgttttc
    32461 ctggacaagt gaaccttctt cggtaaaaat gccgttaaaa aaagttaatt ccgtttacca
    32521 taaatagttg caaagagcaa aagaaatcac cgctttaaaa caggaaaaga taaaatggac
    32581 gtttttgttc gtttgatcgc tgtttgatga attcggaaaa ttcaaaaaag tctgcgacaa
    32641 aaaacattga caaaaacagc aatcaatata taggaataca aaagttgccc ggagaggata
    32701 cacgatgttt ggcataggga tgcaggaact gataattatt gcgataattg ctcttctggt
    32761 tgtcggtcct aagaaactgc ccgatctggc caagtctctt ggtaaaggct tcagcgaatt
    32821 ccgcagagcg gctgacggag tgactgaaga tatcaaggac gccgtcaaag tggatgaaaa
    32881 accgaagaat gacgacgaat ggaagaattc tcttttaatg aagaagcccg atcatgacaa
    32941 cgtaaaatcc gattcatctg aataaatcca aacgataaac tgattcgctc tgactatcaa
    33001 cgctgcaaaa ggcaaaacgt caaactatgg acaataccca ggccgccgaa gatcaaaaaa
    33061 tgtctctgac cgatcatttg ctggaactgc gcaaaaggct cacccgttct ttaattgcgc
    33121 tggcggccgg tttcggtgtc tgttattatt ttaaagacct gatttttgat gtgatgaccc
    33181 gtccgctgac tcaagtgttg tcgaaagaca gtcatcttgt ctatatcggt ttgacggaag
    33241 cctttttcac gtacatgaaa atcgcttttt ttgcttccct gatcattacc agtccctaca
    33301 ttctgtatca ggtatggaaa ttcctttcac cgggactgct tccgaaagaa aagcagtatg
    33361 ttgtcccgtt tgtcatttct tcatctctgc tttttatcgg aggcgttctc tttgggtatt
    33421 ttatcgtttt gcccacggcg ttcaaatttt ttgtcagttt caataatgaa tacttgcagg
    33481 cgatgctttc gtttaaggat tatttgtctc tttttgtaac gttcctgctc gggttcggtt
    33541 tgtcttttga attgccgatt ttcatttttt ttctgaccaa actggggatc gtcaatgcag
    33601 ccaagctttc cagacagagg cgatacgcga ttcttgttat ctttctggtt gccgcagtcc
    33661 tgacgccttc gccggacgcc ctgagccaga tattaatggc aattcctctc atgatcctgt
    33721 atgaaataag catatttgtt tccaaatttg ccgagacgaa aaaagaagaa tcccaaaatg
    33781 acgaaggata atctgtaaaa tgtttctaaa tcgtaaaaag tcaaacagaa aaaacgacgg
    33841 agaagctaaa aaactttctt cgctttttaa tttttttctg ggctttgtcg tcttcattct
    33901 ggtgatgaca gttttcgggg tcatcggatt ctatgctttt acgatggatt tgcccggtat
    33961 cgatgcgctc aaggattatc gtcccagcat ttcttcccgc gtttatgacg ataaaaacga
    34021 actgatcgat gagttttttc tggaagacag aaaactcatc aagattaagg aagttccgcg
    34081 agttgttgtt caggcttttg tggcggcgga ggattcccgt ttctttgcgc acaagggttt
    34141 cgatctgcaa agcattttcc gggcggtttt caaaaacatc gaggcgggac atatcatcca
    34201 gggcggcagc acgattaccc agcaggttgc caagatgatg tatctttctc ccgaaaagaa
    34261 atatacccgt aaattcaagg aagctattct agcttacaaa attgatcggt acctgaccaa
    34321 agacgagatt ctgaatttat atttaaacca gatttatctg ggccatggta cttacggcat
    34381 agagtccgct tcgcttggtt atttcgggaa gagtgcccgg gaactgaaat tgcacgaagc
    34441 ggctcttctg gccggtatgc ccaaagctcc cagcaattat tcccctttcc ttcattatga
    34501 caaagccaaa caaagacaat attacgttct gacgcgcatg aaagaagacg gctatatttc
    34561 ccaggaggaa atggaaaaag cgtatgctgc gccgatgaat ctgcgatcca tccgccccaa
    34621 ggacaagatg gccgcctatt tcgtcgagac cgttcgacgg tatgttcagg aaaaatacgg
    34681 cgccgatgtt ttgtacaagg aaggtctttc catctatacc acgctggatc tgaccgcgca
    34741 gaagtacgcc ggggaggcga tggagcgggg cttgaaggaa ctggaggaac ggaataaata
    34801 caaatccggt ctcgtgcaag gcgcgctttt ctgcatggac gtcagaacgg gtgccatccg
    34861 tgcgatggtc ggcgggcgcg acttcaatca aagcgagttc aaccgcgcga cgcagtcacg
    34921 acgtcaggcc ggttcagcgt tcaaaccgat catttatacg gcggcttttg ataaaggcat
    34981 gacgcctgca accgttattg acgattcacc gcttgctgtg gatgatgttt cgcagcctga
    35041 cggcatctgg aggccgaaaa actttgacga caaattcatg gggccgatca caatgagaac
    35101 ggcgctcgtg ctgtcccgca atgttgtaac ggtaaaaatt ttgcaggaaa tcggcattga
    35161 ttatgctgtt tcgtacgcca tgaatatggg aattacatcc cctctggtgc gaacactgtc
    35221 tttggcactg ggcgcatccg gggtaacgct gcaggagctg gttcaagcgt atggcgtttt
    35281 ggctaatcag ggacagaaag tttctccttt tttcatcaaa aaaatcgttg atcgcaccgg
    35341 caatgttttt gaagaaacaa aagttcagcc ggaacaggtg attgatcccc gcatcgcatt
    35401 catcacgact catgtcatgc aggatgtcgt aacaagcggc accggtacga gagtcaggag
    35461 catagggcgt ccggtggctg caaaaaccgg aacgaccaac gatacgcgtg atgcctggtt
    35521 tatcgggatg acgccttcgc tgatcaccgg agtgtggatc ggtttcgatc aggaagcctc
    35581 gctgggatca caggaagtcg gcggacgggc ggcggcaccg atctggcttt attttatgga
    35641 aaaggccttg cagaacacac cggtggagtc ttttcccgtt ccggaaggca tcgtctttga
    35701 aaaaatcaat ccgaaaacgg gagagcctgc cggaccttcg gaaaagggga ccaatgaagc
    35761 cttcctgttg ggaacgtctc ccagcagcac tctgggcgat tctattaaag gtttattccg
    35821 gtagccggga gttattcctg gggcgtgtcg acgatccgga aagaatgtcc ggcaaaaaat
    35881 attgttgaca aagacagtgt ttttcgttat tgaaagcacg aaataattca acggaatgat
    35941 gtgtttatgt ttcttaaagt tttacaaagc ggcattatcc gggtactgaa taaagtactg
    36001 gtcctcgtag tagtcgtact tacggggtct gctgctctgt atctttaaga aaccggaata
    36061 aaagatacta gccgcaggcc cctgaagccc gcggctttgt tgtttttggc ataaagccgc
    36121 tttttctcaa ggaaacaggc ggcttttttt tgtgagttcc aaatttcaga agcggagcac
    36181 gctgaaattt gcagaacgaa caatccccgg agcgaagcgg agtgggatcg gagactcaac
    36241 tttcaggaac gcatatcctg aaggtcgcta taggtggaaa tttgtacggc ggattaattc
    36301 tgggcagggt aagcgggtta agtgatcttt aaaaaagcga aagagacatg gcctgaaaca
    36361 agaccgtaaa aaattaatta taaatcattt taaaggggaa aaggaatgaa actgaagggg
    36421 acagaaatat tgctgaaagc gctcgaagag gagaaggttg aaattatctt cgggtatccc
    36481 ggaggcgctg ttctcgatat ttacgatcag atatacaaaa gcaacatgaa acacattctg
    36541 gtccggcatg agcagggagc cgtgcacgca gccgacggct atgcccggtc caccggaaaa
    36601 gtcggcgttt gtctggtgac gtcgggtccc ggcgcaacca acacagtcac cggcattgcc
    36661 acggcgaata tggattccat tcctctggtt gtttttacgg ggcaggttcc cactgcgctg
    36721 atcggcaacg acgcttttca ggaatgcgat atcaccggca taacccgtcc gtgcaccaaa
    36781 cataattttc tggtgcgcaa tattgacgat ctcgctgcga cgatcaagga agcgtttgat
    36841 attgcccgct cgggaagacc cggtccggtg cttgttgatc tgcctaaaaa tgtgatggcg
    36901 gcccagacgg aatatgatcc ggccaacatc aaaatcagaa atcatgaggc cgcgtacaaa
    36961 ccggctccga aaaagatggc cggtttactg gatttgctga cagcggcgaa aaagcctctg
    37021 attatgaccg gcggcggtgt cattctgggc agggcgtcat cgctcctaac ggagtttgcc
    37081 aggaaatatc agatgccggt gaccggaacc ctgatggggc ttggttcttt cccggggact
    37141 gatccgcttt ggctgggcat gctgggcatg cacggaacgt actatgccaa catggcgatc
    37201 agccattgtg atctgctcat tgccgtgggc gtcagatttg acgaccgggt aaccggcacc
    37261 atcgatacgt ttgccgccaa ggccaaaata gttcagattg acattgatcc gtcatccatc
    37321 aataaaaacg ttgccgtgga tctgccgatc atcggcgata cccaggcggt gctgaaggac
    37381 ctcatcacat ttctggacga acacaattac gcttatgaca aggatgcaag aaatgaatgg
    37441 ctgaatgaaa ttgccggctg gaaggaaaaa gtgccgctga cctattgcca gaacggcaac
    37501 gtcatcaaac cgcagtctgt catcgagaca ctgtataagt tgaccggcgg cgacgcgatt
    37561 attacaacgg aagtcggcca gaaccagatg tggaccgcgc agttcttccc gtttgacaag
    37621 cccaatacgt ttatttcatc cggcggactg ggaacaatgg gattcggttt ccccgcggct
    37681 atcggtgtga aatgcgcttt tcccgacaag cacgtggtgg acattgccgg cgacggcagt
    37741 atccagatga atatccagga actggccacc gccgcgcagt ataaaatcaa tgtaaaaatc
    37801 gttctgctga ataacggcta tctgggcatg gtgcgtcagt ggcaggaatt gttttacgaa
    37861 aaacggtatt catttacaga tatgacctat gcgcctgatt ttgtaaaact ggctgaggcg
    37921 ttcggcgtcg tgggcttgcg cgccacaaaa ccggaagacg tggagcctgt gttaaaagaa
    37981 ggactggcta ttgacaaacc ggtgctgatg gattttcgtg tctcccggga agaatgcgtt
    38041 taccccatgg tgcatccggg cgattcgatc agcaacatgt cgctggggag cagagaaatg
    38101 atcaattgac gatttcgtga aaagtccaga tgctgcgttg cgcttcatcc ttcggcgttg
    38161 cggcgtatgt gaaaaatacg cctcacttct caggcttcgc gcgccttgcc tctgtagctt
    38221 ttgacaaagt ttttaaataa gaagtattaa ataataaata atatgatgag ggagaagatg
    38281 gaaaagaagg aacatatcat ttcaatactg gttcacaaca aaccggacgt gctggcgaga
    38341 atcgccggca tcctgggcgg taagggttat aacatcgaaa gcctgtgcgt gaattcaacc
    38401 acacattacg aagtatcaaa aatcgtgatg accaccgtgg gtacgcagga caccatcaac
    38461 agaatcgaag ggcaattgcg aaggctggta gatgtaattc aggtggatga tttgaccaat
    38521 gtggaatcga ttcaccgcga attgatcctc gtgcggttaa atctggcggc cgataaaaaa
    38581 gaacagataa aaaaagctgt tgacatgaat aaatggaagg taatagtcaa tgccgacacc
    38641 tacgtgattc tggaaataac cggtgatcgg tcacagattg attttgcgct ggccagactg
    38701 gagccgctgg gcatagccga tatcacaaga accggcattg tcgctttaaa tttggaaaac
    38761 taaattataa tattttattc aatagggggt taatatggca aaaattaatt ttggcggtgt
    38821 tctcgaagat gttattacgg cgaaggaatt tcctctgaaa aaagctcaga aagttttgaa
    38881 gaatgatgtg gtggcagtgc tggggtacgg cgtgcagggg ccggctcagg cggctaatat
    38941 gaaggataac ggcattaatg tgatcatcgg ccaggccaag gaagatacgc gctactggaa
    39001 aaaggccatt gccgatggat ggaagcccgg caagactctt ttcccgattg aagaagcggt
    39061 caagcgcggt accatcattc agtatctggt ttctgatgcg gcgcagaaac ttctttggcc
    39121 caaggtcaag gctaacctga aagagggcga cgctctatat ttctcacacg gattttccat
    39181 tgtttacaga aagcagaccg gcgttatacc gccaaaattt gttgatgtga tcatggtagc
    39241 tcccaaaggg tcgggaacat ccgtccgccg gaatttcctg gacggcagcg gtatcaactc
    39301 cagctttgcc gtgcagcagg attatacggg acgcgcaatg gaaagaacgc tggccctggg
    39361 catcgccatc ggctccggat ttttattccc gacaaccttc cagaacgagg tttacagcga
    39421 tttgaccggt gaacgcggcg tgcttatggg tgcgctggcg ggcatcatgg aagcccagta
    39481 caacgaactg cgcaaacacc gccatacgcc cagtgaagct tttaacgaaa cggtggaaga
    39541 actgacacag agcctgatcc gtctggttgc agaaaacggt atggactgga tgtatgccaa
    39601 ttgctccacc acggctcagc gcggcgctct ggattggcgt cataaattcc gcaaagcggt
    39661 tgcacccgtg tttgccgagc tttataagtc ggtggcgagt ggcaaggaaa cagccattgt
    39721 cctgaaggcc aacagtgctc ccgattaccg ggtcaaactg gaaaaagaac ttaaacagat
    39781 gcgtgattcg gaaatgtgga aagccggcgc tgccgtgcgt gaattacgac cggaaaaacg
    39841 aaagaagaaa tagacgactc cttaaaaagt tcattttacg ccctctccct tgaggggaga
    39901 ggggcgggga agtttctatt agaaatttat tttatcaagg gagagataac catgcgaagc
    39961 gatctcatga aaaaaggttt cgagcgggcg ccgcatcgct cgttgtttaa ggcaatgggg
    40021 tatacagatg aagagctggc caggccgttg atcggcgtgg tcaattcggc caatgaaatc
    40081 attcccggac atatccatct ggatttgatt acgcaggatg tcaaggcggg aatccggatg
    40141 gccggcggaa caccggtgga gttcccggtc attggcgtct gcgacggcat cgccatgggg
    40201 cacgaaggta tgaaattttc gctggccagt cgcgaactga tcgccgattc catcgagatc
    40261 atggcgatgg ctcatccttt tgacgcgctg gttttcattc ccaactgcga caagattatt
    40321 ccgggcatgc tcatggccgc tctgcgtctg aacattccgt ccatttttat cagcggcggt
    40381 ccgatgctgg cgggaaaatt tcaggacaag accgttgacc tgatcagtgt ttttgaaggc
    40441 gtgggcgccg tcagggcaaa aaaaatcacc gccgctgaac tgaagcaact ggaagactgt
    40501 gcctgccccg gctgcggttc atgttccggc atgtacacgg ccaattccat gaactgcgtc
    40561 accgaagcgc tgggtctcgg cttgcccggc aacggaacga ttcccgccgt cctggccgct
    40621 cgtcaccgcc tggccaaacg cgccggaatg aaaatcatgg atttgctcaa gaaaaatatc
    40681 cggccgcgca acattgccac attggctgca tttaaaaatg ctatcgctgt cgacatggcg
    40741 ctgggctgtt ccaccaacac ggttttgcac attccggccg ttgcccatga agcgggcatc
    40801 aaactcgatc tggatctttt caacaggatc agtgaaaaaa ctcccaatct ctgcaaattg
    40861 agccccgccg ggcgtcatca catcgaagat ctggacgcgg cgggcggcat acaggctgtg
    40921 atgaaagtca tcagcgcgct gggtgtcatt gatgaaaaag ccatcacggt aaccggcaaa
    40981 accgtcggcg ggaatttgaa agatgcccgt gtgttgaata cggatgtcat cagaactctc
    41041 aagaatgctt attctcagca gggcggtatc gcgattctgc gcggcaatct cgcgccggac
    41101 ggagcggtag tgaaacaatc ggcggtcgcg ccggccatgc agaaaaacga aggcaaagcg
    41161 cgtgtgtttg actgcgaaga cgacgccatt aaggcgattc ttggcggaaa aatcaaaggc
    41221 ggcgatatcg ttgtcattcg ttacgaaggt cccaaaggcg gtcccggcat gagggaaatg
    41281 ctttctccca cttcggcaat cgttggcatg ggtctggata aaacggttgc tctcttaacc
    41341 gacggacgtt tcagtggcgg tacacagggc gcagccatcg gccatatctc accagaagca
    41401 gcagaaggcg ggccgatcgc gctggtgaaa gaaggcgaca tcatcgccat cgatataccg
    41461 gccaagaaac tgaacctgaa agtcggtgct caggaactgg ccaaacgcag aaaagcattg
    41521 aaaccattca agccgagaat tacgacgggt tatctggcga ggtatgcgaa aatggtgacg
    41581 tcgtcctcga cgggcgccat cttcaaagac tgataccaat tttgatttct tcggctaact
    41641 ttgttggcag cgtcgtcttc ctcgacgtat tatcatatac gtctgtggag ccgactcctt
    41701 gccgccttga tatgatcttt gatttggaag cggaatacaa aaggtatgtt attgctgggt
    41761 tggaagtcag gcgaatgcct gattacccgg caatttatat ttgtagctca ttcgagtttc
    41821 tttattaaag tacgttcttt aaacattttg agatgattat ttgttattac ttactaacta
    41881 actgggaatt aaattttttc atgagggatg tttcattaag attctcaaaa gtcatcaatg
    41941 tgtaaaaatt agaaactgca cgatcaagaa tgttttgcgc ttcttctttt agattacatt
    42001 gaatatttat atcatctctt cctttcatgt gttttgaacc atttttaact cgattgattg
    42061 ttttgtacat actgtctctt gaaccttttg aattatacaa taattcgcca attaatagcg
    42121 atgaatccaa catttcctca aatgcattag gttgtttatg aagcttgagg tacaccgcga
    42181 gtatttcttc cgctgctcct gctaaagtta tgactgagaa ataatttttg tcttcgaaat
    42241 aaagcattaa cgctgtatcc aattgctgag tagcaatttc taattttgaa tagctatgaa
    42301 tagtcatgat atattctcag gcccacaata attaataact ttactttata taaaatcctt
    42361 gttgggattt actcggtgtc ttcgaagccg agccaggcga gagctttttt cttgtcgttg
    42421 aagaattcga tgcgggcgcc tctgttatag gcaacatttt taaaaaattg agtaatgtcg
    42481 tcaggagtgt aatcgtcaat acagatggcc agagtcattc ccggaatgga ctccgccgct
    42541 tgagcgccgg attgataagc gtccatggaa gtaattcttc ttttctggtt ggagccttcc
    42601 gttaagactt tcaggcattt atatttgcgg caggccttgg ctaattcagc ccataggatg
    42661 tcattgcttt ccggcgaaat ttcataatcc ggtgcatgaa ttagatgaat gtacctttct
    42721 ttaaattcaa gagaaaaatc tgaactcatg gagcacttcc ctaaaaagaa aattgatagt
    42781 atttgtgttt gatcgattta tacggatttg tttcagtgat gatttgacat caattttcct
    42841 caaaaaatcc tttatgaaaa catacaacaa aaggcgtttt taagcaatcc tgtaatttcg
    42901 tcattaattc ataaccttaa ctatcagacc acagcagaaa aattcttttc acagaagatg
    42961 tgatcatgct attcctctaa ttttcatgtc gagtttccag aatgccgtgc tgattctgtc
    43021 gataatgtcg tccagttttt cctgaccaat gatttcttcc ggatggtttt catcctgcca
    43081 tttgcatatc ggcgggatgt aaattgtcca ctcgagttct ccaatagcgt ccgaatagtc
    43141 ttcgatttca aatgtgatgg ttttgtccct ctcctgatat cgtaaagcat ccatggataa
    43201 aatatcgacc cgccagccca gagagctttc aactgtatgg tcatccgtgg ctgttatgat
    43261 ttctttcata aagataaacc ggttgtgttt tagacgtttg catccaaaaa tcagtaccag
    43321 tccgcaagca tcaatccggc gctgattcga ttgacgtttt tattatagtc gagcaggctt
    43381 tctccgtat
//
LOCUS       DAOSME010000014        43130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1151_length_43130_cov_19.567707, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000014 DAOSME010000000
VERSION     DAOSME010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 43130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43130
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1151_length_43130_cov_19.567707"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLG98_03495"
     CDS             <1..363
                     /locus_tag="PLG98_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE32346.1"
                     /translation="KGNPGFEKDFVESVKEGIMKQEQYDWFCKATNSLYGATPERQLA
                     FACKNTGFFAMALMLAAKDAGLDSHPMDGFDIDGVHKAFNIPDQYWIPCLLAIGHFDK
                     TKTLLPPKWRKSYEEIVY"
     gene            431..682
                     /locus_tag="PLG98_03500"
     CDS             431..682
                     /locus_tag="PLG98_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOE32347.1"
                     /translation="MAAKIKIYTLSTCSHCKATKKFLNDNSLPFDCVDVDLLQGDARQ
                     KMLEEVMQYNPDRSFPTILIGDKVIIGFKENAIKEALGI"
     gene            682..1014
                     /locus_tag="PLG98_03505"
     CDS             682..1014
                     /locus_tag="PLG98_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOE32348.1"
                     /translation="MTPAELYDMMKKVQEPKGYFFNKDKDWVLSILGDLLVNKERYGY
                     MCCPCRLAAGNREKDADIICPCQYRPDDVREYGSCFCNLYVSAEWNEGKIEHQYVPER
                     RPKEKIGF"
     gene            1277..1426
                     /locus_tag="PLG98_03510"
                     /pseudo
     CDS             1277..1426
                     /locus_tag="PLG98_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(1662..2426)
                     /locus_tag="PLG98_03515"
     CDS             complement(1662..2426)
                     /locus_tag="PLG98_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE32349.1"
                     /translation="MTYHSLIVEKKKKYAVIKLNRPHEMNAISKEMHQELYDVFADLE
                     TDSDVRAVVLTGGEYVFSAGMDIKEMNNLPDKESDKFLKSMMMYLKKIYAFKKPVIAA
                     VGGIALGGGFNLVTVCDLVVASESAIFCHPELKFGFNPFFYPLSQIVGITKAKEIVML
                     GEPIGANEAMKLGLVNKVEPPERFMEVAESIADTLSQRSPRAFEELKNLCSIVPRMDK
                     MAAMDIESSICALLFTRDERKEQMREVLSKDKSGRK"
     gene            complement(2423..2707)
                     /locus_tag="PLG98_03520"
     CDS             complement(2423..2707)
                     /locus_tag="PLG98_03520"
                     /inference="COORDINATES: protein motif:HMM:NF001222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOE32350.1"
                     /translation="MTKNDLIKKLQEELKTYSLKDVTYVVNIIFDSMIDAIKRGERIE
                     LRGFGSFEVRERKPRMGRNPKTGTQVKLTERKVPFFKTGKELRIMVDNKK"
     gene            complement(2721..3617)
                     /gene="sppA"
                     /locus_tag="PLG98_03525"
     CDS             complement(2721..3617)
                     /gene="sppA"
                     /locus_tag="PLG98_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOE32351.1"
                     /translation="MRKHPVLFGMLILFVFGLIFYLSFYNAGFNMGKTSRFSLKNKIG
                     VVPVEGMIADSLEIVEQLDKFGKDGSITAVVLRVDSPGGGVAASQEIYDAILELKKTK
                     KVVVSMGSIAASGGLLIACAADKIVANPGTITGSISAIMQFANFEELLKKVGVKSAVV
                     KSGEYKDIGSPLRDMTPEEKVIVQDLVDDIYNQFVDVIVKERKLSREKVIEIADGRVF
                     SGRKAKELGLIDYLGDMSSAAKLADKLAGGNGEFDLVYPSKKRASIFDYVLEGAASKI
                     SASLKEKMEMNSGLSYLYYPGR"
     gene            complement(3621..5381)
                     /locus_tag="PLG98_03530"
     CDS             complement(3621..5381)
                     /locus_tag="PLG98_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOE32352.1"
                     /translation="MVNNNNSNLSNKNEAGEKSLPKNNITSGKEEMDFKELYEQSLNQ
                     FQYGDIATGKVVQIKDDRIMVDVGWKTEGFIPIDEVRDAQGNVTISIDDEIDVFIDRR
                     DSEGNLVLSRDKASKVKIWDEIKNACENDTVVEGVVVEKVKGGLSVDIGIIAFLPGSQ
                     VDIRPVKDLDKFVGQTLDFKVLKFDRKRNNVVLSRRSIVASEREAEKQDILKNIQEGN
                     VVEGIIKNITDYGVFIDLGGVDGLLHVTDISWGRIVKPSEMFQKGDKITTKVLSFDAE
                     KERVSLGLKQLTANPWEKITETYPVNSIVRGKVVNLTDYGVFVELASGVEGLVHISEM
                     YWTREIKHPSKVLNIGEEIDVKVLEVNAQNKRISLSLKQTTPNPWEKLKEKYPVGTIV
                     KGIVRNITNFGVFVGIEEKIDGLIHVSDISWKHRVNHPSEYFKKGQEVEAVVLNIDVQ
                     NEKFSLGVKQIEKNPWDELAQKYAPGSVVTGKITNFTDFGIFMEIEEGIEGLVHISEI
                     SQKRVKTSSELYSVGDTVSAVVKSIDPKSKKIRLSIKELEAPAPTPSSNQYINNTENV
                     GSNLSQALADVKINTKQKGE"
     gene            complement(5492..6160)
                     /gene="cmk"
                     /locus_tag="PLG98_03535"
     CDS             complement(5492..6160)
                     /gene="cmk"
                     /locus_tag="PLG98_03535"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOE32353.1"
                     /translation="MKKKAVITIDGPAGAGKSTISKILAKRLGYIYLDTGALYRGLAY
                     KALKIRISIDSSAELSELCSTTKVDLKNVNGRMRVYVDGEDVENRIRTEEVGLIASKI
                     STFVVVREKLLDLQREAGLRGGLVAEGRDMGSVVFQDADYKFYLDATLAERTKRRHKE
                     LLLKGSTAEYQSIQNDMSIRDKQDQERAIAPLKPPEGSIIVDSDNLSIEEVAEKIMSM
                     ISKK"
     gene            6255..7697
                     /locus_tag="PLG98_03540"
     CDS             6255..7697
                     /locus_tag="PLG98_03540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE32354.1"
                     /translation="MCKFIQNQYIYLGMIVLMLLLTSDICRAEFTIEDENKLGREFYE
                     QLEQHQMILHNKVLTDYITGIGNLILDQTTRTPFKFNFFILDSSAINAFATPGGYIYI
                     NKGLLLVAENEAQLAGVIAHEIGHANARHVASIISKSQKLNMAALAAMLAGVFLGGGG
                     EASAAITAFSLAGSSSMTLRYMREHEEEADRLGIDYLVHAGYNPSAMVDFLKIMKQYE
                     FLSKTMPSYLQTHPGTDDRIIYMDGLIMTRYRQPGKNSIIGNLCRVQASVPLSASELS
                     RRQRQLETSLAKDPQNTDLLYNLAMIEDQLGANSSALEHLQKALVQSPDDHEILKSIG
                     LLHLKTGRYEQAQNYLLRAKKSDPRNDEITFALGKTYFSSGKYREALDCFLNIRDRAS
                     NEAANLNYFTAMSYGKLQNKGESHYYFGLHFKQANKKDSALFHFREALNFFPPGSERN
                     AEIHRAVKELTAGKDKQKRTIKKDEQNSFL"
     gene            8087..8761
                     /locus_tag="PLG98_03545"
     CDS             8087..8761
                     /locus_tag="PLG98_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOE32355.1"
                     /translation="MNILIFGPNGSGKGTQGAIVQKKYGVPHIETGVIFRQNISKKTP
                     LGEKAKAFIDRGELVPDDITIPMILDRLKEDDCKKGWLLDGFPRNLAQAEALWDALQK
                     ENIKLDSVIEIVLDRQIAKNRIMGRRLCKIDNNHPNNIFIDAIKPHEHDGKTVCRVCG
                     CEDLSTRADDQDTAAIDKRHGIYYDTKTGTLAAVNFYKAKVKVIEVDGSVGVKEVTDE
                     LMKKLG"
     gene            8829..10625
                     /gene="lepA"
                     /locus_tag="PLG98_03550"
     CDS             8829..10625
                     /gene="lepA"
                     /locus_tag="PLG98_03550"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOE32356.1"
                     /translation="MENIRNFSIIAHIDHGKSTLADRLIQFTGVCNERNFQDQILDNM
                     DIERERGITIKSNAISLPYRAKDGKTYILNLIDTPGHVDFSYEVSRSLASCEGVLLLI
                     DAAQGVQAQTLANLYMALEHDLEIIPVINKIDLPSADIDRVLEEIDSELGLDPFTHIK
                     CSAKEGAGIEEVLEAIVLRIPPPKGSPANPLAALIFDAKYDSFRGTIIHCRVFEGTIK
                     SGDIIKFMSNGATYKVEEVGHFLLNRTKREKLSAGDVGYIIAGVKTVSDVRTGDTITL
                     QNRPCVQPLPGFKDVKPVVFASIYPIASDDYQDLADSLEKYKLNDASFIYEKDSSAAL
                     GQGFRCGFLGLLHLDIVQERLEREFNLSIILSVPSVRYRFSLTDKTVIYVDNPAHYPD
                     PIEIDKGEEPYIRSSMMLPERYLGAVMKLCMEKRGVNSVMNYTGPGRVELSYEMPLAE
                     VIYDFYDRFKSVTQGYGSFDYDIIDYRESNLVLMDILVNGEKVDALSQIAHRDRARAR
                     ALLACKRLKEEIPRQMFKIAIQGAIGGEIIARTTISAFRKDVTAKCYGGDITRKRKLL
                     EKQKAGKKRMKMIGSVSIPQSAFLAVLKSDTD"
     gene            10664..11791
                     /gene="hemW"
                     /locus_tag="PLG98_03555"
     CDS             10664..11791
                     /gene="hemW"
                     /locus_tag="PLG98_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOE32357.1"
                     /translation="MRSEKAGLYIHVPFCLSKCAYCDFYSVKTLHLIPDYIAAVQKEM
                     KLYRNVFSSFDSIYIGGGTPSLLAPQYLDEIFTALNKYFIIDKNAEITLEANPGDLSP
                     VYLKALRDTGINRLNIGVQSFNDKILKCLGRRHSAQEAMASINAARKAGFRNLGIDLI
                     YGVYGLGLQGWEKILQQALILAPEHFSCYQLSLHEKTPLHKTYLSAGWHLPDDNAELK
                     FFLTTSEILENVGYRHYEVSNFARKDKYTSRHNQKYWQHVPYLGLGPAANSFLNNQRW
                     WNKASVKRYLNAMARGKTPVEASETLTVNQLRLEALFLGLRCKSGIDLKRFKDQYGTD
                     LMTEKKSIMDALMKNKLIELKNGFLCPTRSGMALADSLALI"
     gene            complement(11889..12581)
                     /locus_tag="PLG98_03560"
     CDS             complement(11889..12581)
                     /locus_tag="PLG98_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOE32358.1"
                     /translation="MMKIGGLQRVSLIDYPGYISAIIFTQGCNFKCPYCHNPELVNPD
                     LFRTPIKVSEVIDFLESRKGKLDAVSICGGEPTIQDNVISFIRQIKKIGFAVKLDTNG
                     SVPNVLKTLIADNMLDFIAMDVKAPLDKYRDIVKANVNQDAIQESIRLVLNAKIPYEF
                     RTTIVESQLDENDILQIGKMINGAGHYVLQNFMAKKTLDKKFAKEKSFSEEKLRKIKK
                     QLEQQIPSVTIR"
     gene            complement(12650..14737)
                     /locus_tag="PLG98_03565"
     CDS             complement(12650..14737)
                     /locus_tag="PLG98_03565"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOE32359.1"
                     /translation="MNLKIRKRDGRLVKFNAEKITNAIAKAGNATGDFDIKEARKLTI
                     KVLNLAEKLFDSKILSVEEIQDVVEEVLLSSPYRKTAKAYIIYRDQHSRLRDIANRME
                     VDLVDQYLKKLDWKINENSNMDYSLQGLNNYISSEVSKVYWLNEIYTPEIHKAHTEGD
                     FHIHDLSLLSVYCVGWDLYDLLLQGFQGVSGKVESKPANHLRSALGQVVNFFYTLQGE
                     AAGAQAFSNFDTLLAPFIRYDNLSMKEIKQALQEFIFNINVPTRVGFQTPFTNVTLDV
                     TVPKYYADQNVIIGGKPQKETYREFQEEMNLFNKAFLQVMAEGDAKGRVFTFPIPTYN
                     VTKDFNWDDPNLQDLWEMTAKYGVPYFSNFINSDMNPEDARSMCCRLRIDNRELEMRG
                     GGLFGSNPLTGSIGVITINMPRIAYLSKSKKDFLTRLEHLMELAKESLETKRKVLEKF
                     TYGNLYPYTKYYLRGVKERFGEYWKNHFSTIGLVGMNEACLNLLGVNVATDKGQVFTK
                     QVLDFMRNKLISFQKETGNNYNLESTPAEGTSYRLAKIDKEKFTDIITASEGKSKKAE
                     PFYTNSTQLPVDYTDDIFEALDLQDEIQVKYTGGTVFHIFAGERIDDPQSVKKLVQKI
                     CFNYHLPYITFTPTFSVCPSHGYIKGEQEKCPTCADDCEVYSRVVGYLRPVKQWNKGK
                     QEEFDLRQEFKFK"
     gene            complement(14931..16964)
                     /gene="fusA"
                     /locus_tag="PLG98_03570"
     CDS             complement(14931..16964)
                     /gene="fusA"
                     /locus_tag="PLG98_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE32360.1"
                     /translation="MASKSKIARTRNIGIVAHIDAGKTTVTERILYYTGKSYKMGEVH
                     DGEAVMDWMPQEQERGITITSAVTTCVWRNHDIHVIDTPGHVDFTIEVERSLRVLDGA
                     VVVFCAVGGVEPQSETVWHQADKYGVPKIAFINKMDRVGADYFNVIGMMRERFSSLPV
                     AIQIPAGSEDNFRGVLDLINQKMIVWNDSTSGSEMSKQDVPSEFADHVKLQRDKMIET
                     LAEVDDSLADKYLGGEEITIAEIEEALRKATISLKIVPVLCGSALRNKGIQPVLDAII
                     NYLPSPEDIPPVKGMNPVTKEEEIRRSSEKDPLAALAFKIMMDEGRKLTYLRIYSGKI
                     QVNNEIYNPIKKRREKIARLLRMHANKRERIDSASAGDIIAVLGLKSTTTGDTLCAEQ
                     KPILLELIEFYEPVISQAIEAKTPADQEKLTAALDKLVEEDPTLKVKYEDETAQTIIS
                     GMGELHLEIIVDRLLREFNCHVNVGRPRVVYRETIQKKVEIEGIFEKELGEKKHFGHV
                     RIALSPRKRGSGNDVIIKLDDSVIPQDFHEVIEEGIREAMMNGVLNGYPVMDTEVAII
                     GGSFREGESSAQGYKIAASTAFRDGCGQAEPIMLEPIMMVDVITPSEFMGDVIGDINA
                     RKGEIQAVNPKGTVSEIKAKVPLKAMFGYSTDLRSSTQGRAVFSMIFSEYNKI"
     gene            complement(16974..17597)
                     /locus_tag="PLG98_03575"
     CDS             complement(16974..17597)
                     /locus_tag="PLG98_03575"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOE32361.1"
                     /translation="MSTKKQLRSESIKEKKIKKSTRVFYLSLIVGGGVVLLVIFFITI
                     FSALFPPVDTDVMTKKERRVVKIYFSDQQERFLKAEKRHVIKESDIALQAQEIVKALL
                     DGSKTGLVNTFPKNVSLRDVKIKDHDVAYVNFSKDLIKAYQGSSAAEMNTIYSLTNSI
                     TQNIPEIKRVKILADGKELSSIQGHISTGKFFSPDLELIIPEQSPNN"
     assembly_gap    17848..17947
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18002..18580)
                     /locus_tag="PLG98_03580"
     CDS             complement(18002..18580)
                     /locus_tag="PLG98_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOE32362.1"
                     /translation="MAKSIKGTKTEKNLLASFAGEAQARNRYTFFASAAKKEGYEQIS
                     RIFLETAENEKEHAKVFFKYLEGGEVEITASYPAGVIGDTISNLKAAAEGENMEWTVL
                     YSDAAKVAREEGFPDVARSFEQIAKVEMFHENRYRKLAENIAKGEVFKKKTATKWHCI
                     NCGYVFEGEEAPKECPACKHPQAYYEVLAENY"
     gene            complement(18638..19210)
                     /locus_tag="PLG98_03585"
     CDS             complement(18638..19210)
                     /locus_tag="PLG98_03585"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOE32363.1"
                     /translation="MKKSFGAKTLIYPTPVWLIGTYDQEGRPNAATVAWCGVCSSDPP
                     ALAISLRKSRYTYESIQHRKAFTVNIATADQVVAADYCGMVSGKKTDKFAAAGMTAVH
                     SNIVDAPYIAECPMVVECRLTDQMEVGIHVHLIGEICDVKVETTLLTEDQLPDIMKIQ
                     PLIYAPENRSYHGVGGYLGEAFSIGKKIMK"
     gene            19393..19578
                     /locus_tag="PLG98_03590"
     CDS             19393..19578
                     /locus_tag="PLG98_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32364.1"
                     /translation="MATIMPEAENVQKAIKWISASLESENLPLQKLIEKAIFKFDLSP
                     KDTEFLTNFFRESRSNK"
     gene            complement(19683..20087)
                     /gene="mscL"
                     /locus_tag="PLG98_03595"
     CDS             complement(19683..20087)
                     /gene="mscL"
                     /locus_tag="PLG98_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOE32365.1"
                     /translation="MAAKNKVSAMMEEFKNFAFKGNVVDLAIGVIIGAAFGKIVDSLV
                     KHIIMPLVSVVIPGEQGYLGWKWIINGKEVPYGLFIGEIVNFLIVAVALYFFIVKFLG
                     MMMKSKEEEAAGPAPLTKDQELLTDIRELLKK"
     gene            complement(20108..20554)
                     /locus_tag="PLG98_03600"
     CDS             complement(20108..20554)
                     /locus_tag="PLG98_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32366.1"
                     /translation="MIHKKHRVSFFLLVLSVLNLISCFGLGPGGTVISKPDQITRTFE
                     TKETAALRAVARVFSEKKMGADTKIDYENKRVDSDYVVSGQWRTKAAAVVRQINWKEC
                     EVTVSVITEKHAEKGWEMRRLLQHPQYDNLFSVIELKIYEEMSRVN"
     gene            complement(20586..21161)
                     /locus_tag="PLG98_03605"
     CDS             complement(20586..21161)
                     /locus_tag="PLG98_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE32367.1"
                     /translation="MDSIANFLFEVGMLSRTPRSGYQFLGSGQESVAEHILRTVFVGY
                     TLCKMDSSLDEMRVLKMCIFHDLPEARTGDMNYVNKKYVKVDEEKAIRELAESLSFGA
                     DIRSAIDEFNQKKTKEALIARDADQIALILQLKECGDLGNKYAEEWIGFAVQRLNTEN
                     GKKLSEKITGTDSSLWWFKEKGDWWVNGNNP"
     gene            complement(21163..21381)
                     /locus_tag="PLG98_03610"
     CDS             complement(21163..21381)
                     /locus_tag="PLG98_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32368.1"
                     /translation="MAQMSSTDLFIREYQDRFEKKIRENEIASLEHWKAQLDKVIAMR
                     PDGVASMQLQITKISEMMSNRIKTLKKE"
     gene            21521..22390
                     /locus_tag="PLG98_03615"
     CDS             21521..22390
                     /locus_tag="PLG98_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOE32369.1"
                     /translation="MPPKPQPEDIAGGQALIEGVMMRHGDKIAAAVRRPDNEIVFQER
                     EYIPLTKRYKVLGWMFIRGTITLFEMMIVGFKALMFSADVALSEEERKPKDWEMYLSL
                     MISFSIAIFFFVVVPAYCFTKMKFYIDNLLLLNILEGCVRLGMFLCFLAATLLMKDMR
                     RVYMYHGAEHKTVFAWENGQDLTVENIKNFSTRHPRCGTSFILFVMIVSILVFSLLGR
                     PDFLHRVLYKILLLPVVSGISYEAIRFTGKYKHSRLVQILSWPGLMLQRITTREPSDD
                     QIEVAIAAMKKVI"
     gene            22568..24289
                     /locus_tag="PLG98_03620"
     CDS             22568..24289
                     /locus_tag="PLG98_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOE32370.1"
                     /translation="MFKRYVLALLIVLASTTLAWNIVEASREDTAQIQLKKTAISRKN
                     LHTYTVKEGDVLINIIKSIPGISEKDIPYTYRLIKDMNPHIANIENLEVGQSIFLPEK
                     PMKMKNDRTNVKAPAAAPKTVSAGEFYYKVKRGDTLYKIIRREFKVSETSIPQVVRSI
                     KVLNPTIKNVNKIYVGTMLKLPGKTVFVQSPDDLTPATRKPAISDTAEQYVQEDRIIE
                     IKDKKMMPFEARLALLKQIITQMNGTVTTTGNYYLPIPQAGQVTIDCSKIPLIEFDDN
                     TTVFLDLENRAHSNLKKMISNNWKNYHLVKISNTDDTITILKKVFSVTNKYSILKSDK
                     PLTFGALPPAEVMVDWLIVKPIPGQQSKSVIQGLRQIYENNVLLPKSIKNYAQKNGLI
                     ITEFSEETGIADKPEELYSLPPIPVFPATNAKEFSHALVSHLGLSAEKDVEIQIFDTV
                     KDGFNLSLKADVLVTKGNNKYVIYSQSLSQQFINALKQAGNETIFVSNSDSTKDIMGN
                     VLSGLDIPFTYGTHAFSGLEKNQAPYALKFSGTRINPDLYVVNFDIDPGLRGLLQEVW
                     SARIARY"
     gene            24493..25464
                     /locus_tag="PLG98_03625"
     CDS             24493..25464
                     /locus_tag="PLG98_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOE32371.1"
                     /translation="MTKAIALFSGGLDSILAAELVRRQTIDVLCLTLTTPFFNAQKAL
                     AASRQFNLPLEVMDITSEHLKMLRSPRYGYGKNMNPCIDCHTLMLNIAGRKMDQTGSD
                     FIVTGEVLGQRPMSQNKQSLNIVAKNSGYRDFILRPLSARLLEPTKAEKENLIDRSQL
                     LAIQGRGRKQQIILAADFGITSYAPPAGGCLLTDPVFTRRLRDLFAHQTEPEIRDYEL
                     LKHGRHFRIHDTCKIIVGRNSGDNEALKHLAASGDVLFNMAHFPGPLVVVPRDSLCDP
                     QIAAALCVHYSDAPPDALQEVICVRDNQSEIVTAAAASKEDCQRWIL"
     gene            complement(25488..27026)
                     /locus_tag="PLG98_03630"
     CDS             complement(25488..27026)
                     /locus_tag="PLG98_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOE32372.1"
                     /translation="MKKKIKPEAHSLFSGKDLQNMERLRLNLNDVERQLTLHRKGPLF
                     LKLNRPCTDHDGIVSFSSARRRALISFYERESCKYDIIKFVPASGAASRMFSEWFSAS
                     EKGGFGSKALDRLFVANLKKFPFFDLIRQNDPGRKLLAEKDVGRLLKFILSSGGMNFG
                     KRPKALIPFHRYSAREIRTPLEEHVCEAALYTLNRGVCHIHFTLSPGYKSGVARYLKK
                     VIAGYEKRNRIRYNISLSVQSASTNTIAVDKNNLPFRDDQGRIVFRPGGHGALLKNLK
                     HLDADFIFVRNIDNAAPETLWEKIIPHRKMLGGLAIRMQEEIFSHIRKLKNNKPDVSQ
                     IRNIANFCSEQLNIVFAEGFDRQPASEKIRTLLSCLNKPLRVCGMVRNENQPGGGPFW
                     VEEKDGTQTLQIVESAHVDTKQTDQAAIWFGAEYFNPVDMVCCIRDYRGRVFDLSDYV
                     NNDTYLITSKSEKGRQLKALELPGLWNGSMAYWNTVFVKLPIVVFNPVKTVYDLLRPG
                     HSVR"
     gene            27181..28302
                     /locus_tag="PLG98_03635"
     CDS             27181..28302
                     /locus_tag="PLG98_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOE32373.1"
                     /translation="MNKIYKFFTLIIMTIALVIAAEETANAQIGWQKSAGQSLIDALK
                     VKEPLTFCNEKVPLKENDVRERLEREMLVSLQNSDDVILWLKRAYRYFPHIEMVLREN
                     RLPDDLKYIVIAESALKPLAASHKGAVGFWQFIESTGKRYNITVNSDIDERRNFFIAT
                     DAALAYLKDLYSIFNSWTLAAAAYNMGEDGLKTEILMQQTNNYYLLHLNQETQRYVFR
                     ILAAKIIMSDPEKYGFHLSKDDLYPPLQFDRVEISADNPVPIQVIAAAANTYFKIIKE
                     LNPHLKGYDLPPGRHSVLIPKGTADGFSGRYENLLNQWHIEKENATYTVQKGDSIYTI
                     AKNLNVSVKAIMIWNDLAGVNQKLSPGDKLVIFTRNRFF"
     gene            28483..29388
                     /gene="nadA"
                     /locus_tag="PLG98_03640"
     CDS             28483..29388
                     /gene="nadA"
                     /locus_tag="PLG98_03640"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOE32374.1"
                     /translation="MTIVDRINKLKQEKNAIILAHNYELPEIQDIADFVGDSLGLSME
                     AAKTDADIIVFCGVRFMAETAKILSPQKTVLLPDKDAGCAMADMIEPEGLRALQSRHP
                     GAVTMCYVNSSARVKALCDYCCTSANALPMTRKIAPDHRDIIFVPDQNLARYVSANAQ
                     CHFIIWEGYCPVHTDILPEHIQQAKRLHPQAKVIVHPECKPEITALADIVTSTEGMSR
                     HAQNSPDKEFIIGTETGILHRLKKENPDKFFYPASDKAICADMKKITLEKVLHSLETL
                     SYEITVAPDIMDKARLSIERMLQTT"
     gene            29410..29790
                     /locus_tag="PLG98_03645"
     CDS             29410..29790
                     /locus_tag="PLG98_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOE32375.1"
                     /translation="MMKKKIISSDTGPISGPYSAAVEANDIIFISGQLPVDPATGKII
                     TDIKIATGQILTNMQALLKKTGLDLSHIVKTTIFLKDIKDFPAVNEVYAGFFPKDPPA
                     RSTVEVSVLPRDVPIEIEAIAVRE"
     gene            29936..32830
                     /locus_tag="PLG98_03650"
     CDS             29936..32830
                     /locus_tag="PLG98_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32376.1"
                     /translation="MPTKHPIKSKNKKTSPYPNEFSLLSEEIIAKAGVGIYIVQNRKF
                     VYTSNLFQIITGFTDDDLIGASPLDRVYPEDQENVRQHAVKCLKKEIFEPYEFRLFNK
                     DNQLRWILETVTPIVYKGQPATLGSFMDITERKMTEEALRQSEEKYRTIIENIEDGYV
                     EMDLKGNFIFFNDAISKMHGFTSIELLKMNYTDVMDEENAAKIYAKYNHVFKTGIPEK
                     DFEYQIITKDGQRKFLETSISLINNSAGRIIAFRGIVRDRTQRKQAEEALRQSEEKYR
                     SILENIEEGFFEVDLAGNFTFFNHSLCSLLGYSREETLGMNYRQYTDKDSAQDVFRTF
                     NGIFRTGMPVKEFDWFIIRKNKTRRYVEASIYLKKDSSGKPTGFQGFTRDVTDRIQAE
                     FQKEAALEALRESEEKYRNILENIQEAYFEVDLAGNFTFFNDALCRMTGCSKEELRGT
                     NYTKFSDEENSKKVFQVFNKVFNTGQPTEGFDWLIIRKDSTKRYIEASVSLKKDSHGH
                     PAGFKGVIRDISERKRIEQELNYMATHDALTGLPNRLMFNQLLNQAILSAKRHKKQLA
                     VFFIDLDRFKIINDTLGHDAGDLLLQEIARRFRHSLRAVDVVGRLGGDEFVILIEDFS
                     DFAQVSKVAHKILSAAIKPMVIMSEECRVTASIGISIFPKDGQDEQSLMKNADIAMYF
                     AKEEGKNNFQYYSKDIKTQSNERLSIETNLRRALERDEFYLTYQARLDFKSNQITGVE
                     ALLRWNNPYLGQVTPTQFIPLAEETGLIVPIGRWVLKTACAQNMAWQKQNLPPICMAV
                     NLSLRQLSDENLLNDIRMALNESGMPPQLLELEITESMVMHNPAKLIQLLTKIKDMGV
                     RLAIDDFGTGYSSLAQIKHYPIDTLKVDRSFIRNLPKDSEDKAITEAIITMGKTLSLT
                     VVAEGVETLEQENYLREHVCDEMQGFYFSKPVKADDFAELLRNHTGAHLT"
     gene            complement(32870..35044)
                     /locus_tag="PLG98_03655"
     CDS             complement(32870..35044)
                     /locus_tag="PLG98_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOE32377.1"
                     /translation="MQTVEKTSYKKDEKKEFTPDEFAKAKTTCEFVIELIKAISRSGY
                     YDSHHPVSQEVKKGLYIAFKKTLGDSSEIMLSCHEYEDKVDIHISGILNEPFNIRKLT
                     QSSTSDLFVPKLKDYFERKSLNSFVIKKNITAEHFESFIDVMSEPIAESADTSKLGEY
                     LTKSLVDRGITEVSTIFKTDIVLSRGKLPWRVSIILRRLAKDLNVVPMFRFATEDKMK
                     VIKKQIVEDIIRPLTNNDLLKDLIVNCDIVVDHVSHFMEVDELEKLIISSLQSDAVVP
                     ISTTVFDIYRDNLMDGVSQEENEQRLERNVYLAKVLNIAAERMIAEKMPDIKNLFEQL
                     FECKIIDSDMLPADMRIDRESAQLADEILSQTENYLENISKASSIEEAESIVLTVKRV
                     IPAFVNAGEWKIIARIVQALNDLSAHHEDMIQESEFLKGMPDSIWEGMEERIADKYIQ
                     GDANLRSKIDEVLLLSNTMCIKTVDLMISKSQEPNILKCAVEILSQKGETGRRWAMDV
                     LGQSNHPLSVLNAALLVIMQVGQKEDSKTVRKFLKHTNASIRARALAAMAKINQQDTA
                     AVIIDALNDEEEKVRHQALSLIEHELPVSVEMLQKIVSFLKERISKKNINANDAGLVC
                     GLLKAAGHLGEVSDRETLENEIIEMAAELLNEKKGILKFMKAEIPREKQDILCACAVS
                     LGKCGGIKSKEFLKTLSRNNNDAVANTAKDAMDILDQKAARQ"
     gene            35310..36242
                     /locus_tag="PLG98_03660"
     CDS             35310..36242
                     /locus_tag="PLG98_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE32378.1"
                     /translation="MMNHRHPLHGRSTGMFRRIILCLALILAVASLVACSLINTLFPP
                     PAKPAKIALVLGGGGSKGFAHIGVLKVLEAQKIPVHMVVGTSAGSVVGSLYASGKSAF
                     ELQEIALKMDKDKIIDYDWKIWSGGLIIGDKLENFINTYVKNTPIEKLKIPFYAVATN
                     IATGEETVFARGNTGMAVRASCSVPAVFQPLKIGNNTYVDGGVVSPVAVNVARRNGAD
                     IVIAVDISGGLQRTVPDGMFDSIKKSVDIMYSKIAEYQIKHADVVIRPNMKHISSTEM
                     EKFNEAILEGEKAATLKLPELQQIMAQLKQERRL"
     gene            36277..37050
                     /locus_tag="PLG98_03665"
     CDS             36277..37050
                     /locus_tag="PLG98_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOE32379.1"
                     /translation="MMIIGARAHNLDEANTLSKMHCPYIEISLDDPAVVSGWLPQLRN
                     ILNRDGITFLAHYPNEDNPFDVAILRNTFLPRIVTLMELSQKLDIHKATIHFWIDKRR
                     LPHDLIRDKLDILSRIVDYGNDFGITVCIENLSERADSFAPAFDAIPDLKMTLDIGHG
                     QLLSRQNTSFRFIEEHFPRIAHVHAHDNHGGKTVSDDLHLALGDGSIDYKRIFKRLIA
                     KGYASTITTEVKPQDLLKSKQRLEDCIRSITPAQTGNGL"
     gene            complement(37019..38257)
                     /locus_tag="PLG98_03670"
     CDS             complement(37019..38257)
                     /locus_tag="PLG98_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE32380.1"
                     /translation="MNEKHTAHLKENYGKNILPLSVLENERMIAGSPNEILYFYRKDI
                     PIGKLKESIIKTIEHYNLFSSRLIAIGENKFALQYCTDGAVLNELPPLDAVSRDLSLE
                     DIKKMMVHVKTLPGEPLFAITGIPVKDGILAGISCSHTVADGISMLLFLYAWMCITEG
                     RPFLPPSPQRLFKGLPVDSDKIHRVFLPSLSELSREIQDKAGHYEKDEKLYTRREFFS
                     DEFLADMKRTAQQENETCSISSNQIIISFLLKKYHDHILRDTDKVRLRNGINLRDLHP
                     DIDPLHLGNATFTSMTEFTRKEIHAMSIAQIANRLKETIAGYRNEKFAKALSYLTEYG
                     IEYNAEVLRQHHKAYNSETDINSTNLTHLSDLESLGLSSNIGTILDVSSVVHAGFVIL
                     KEKNGQIFADVTSRYPFVQE"
     gene            complement(38374..38922)
                     /locus_tag="PLG98_03675"
     CDS             complement(38374..38922)
                     /locus_tag="PLG98_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32381.1"
                     /translation="MGRLTGAENYVLKKSDSNFLIALFAVIYFTSQIILGTIFHKIGT
                     LQAFMLQTTLSSDKFKDIASGWIASGQIGIYYQHFYLDYFHPLWYSIFLSLLVARAFK
                     MNHVNPKFNGVVLTPFIAGLCDLFENTMHIYFLADLDRATPVLVAISGLATNTKWILS
                     LSVTALAIVLIAYRIIKRRIIK"
     gene            complement(39021..39962)
                     /locus_tag="PLG98_03680"
     CDS             complement(39021..39962)
                     /locus_tag="PLG98_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOE32382.1"
                     /translation="MQKPTGFDFLKKHFIVIMGLAGFVFALAVTDMPTLRDYFHADNI
                     GESYAALQSRVQDHIPLPVVYGEKLNPESIIRLTNKARTDNGLAELAENRLLNQIAEA
                     RARDMLGKQYFAHVSPTGEQASDLAEDVGYAYKIIAENIGKGVFHSNQKIVEGWMQSP
                     GHRDNMLSEDIREMGAAVLEGKMKGRPTYVAVQIFGLQSLPTPHNICVAPSEDLLRDI
                     EVKKAQIAALQNQLDRLKNELDAQQAEIDTDKKYTYDDPQKIQNVNEKIYAYNEKSRW
                     YNKIVGEAKGKTAVLSSMIDEYNRAAQFYRACTEKTP"
     gene            complement(40057..41502)
                     /locus_tag="PLG98_03685"
     CDS             complement(40057..41502)
                     /locus_tag="PLG98_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE32383.1"
                     /translation="MQKNAEHIALISDKQHITYEQLHRAVGEVAGFMQQRGIGKGDKV
                     TVMLPNIPEFVFCYFGIVKNGAVVVPINPLTTPSELIYLLNNSDSKILITQSSQLKKY
                     HEIKDNLSTCRQVIAVDALSPEGQPFPGAESQGASFTPVEIKPEDPAVMIYTAGLTGR
                     PLGAVLTHHNLYSQAVVTQSIAQRTPDDVALALIPLFHAFGATANMLLTIRAGCSIVM
                     MDRLTMDGLFGSIEREKITYISAVPRLFMGMIFYEKASRYDTSSLKLCVTGGSAMPAD
                     FMHVFEEKFNLKLLEGYGLTEASPVCSFNRFDQVYKPGSIGTAITGVEIKIGDDQGRE
                     LPRNQIGELIVRGENVMQGYYKNEAATAAVIKNGWLHTGDLGRMDEDGYIFITGLKKR
                     MIITSGFNVYPLEVETVLNGHPAVKAARVSGKEDLMRGEIVKAYVILKEGAVADDKEI
                     IRYCKTYLSNYKVPREVEFVQEIPASADIEY"
     gene            complement(41709..42332)
                     /locus_tag="PLG98_03690"
     CDS             complement(41709..42332)
                     /locus_tag="PLG98_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOE32384.1"
                     /translation="MNVLLINGSPNKKGCTYAALSEVAKALKKQDIDAEIFYVGNKPI
                     RGCTACGKCSETGKCIFDDDAVNVAIGKAAEADGFVFGSPVHYAGASGFITSFLDRCF
                     FAGSDVFAYKPGAAVVSCRRGGATAAFDQLNKYFTISNMPVVSSQYWNMVHGNTPEEV
                     LQDLEGLQIMRTLGQNMAWLLKSIAAGRKAGIVRPEPEEHIWTNFIR"
     gene            42721..>43130
                     /locus_tag="PLG98_03695"
     CDS             42721..>43130
                     /locus_tag="PLG98_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32385.1"
                     /translation="MYSTREQKFILKEWLDLSKVFGAGRFQGGYSVDDIDSILENAMK
                     IAKLSIAPTSDDGDNLHAVYKDGKVTTPESYKKGYWAIQENGLGSSNADHTDPSSMPL
                     CVMGGTNEYLAAANASLGPYAMATTGSAGLIRDFG"
BASE COUNT        11426 a        10729 c         9195 g        11680 t
ORIGIN      
        1 aaaggcaatc ccggttttga aaaagatttt gtcgaatcgg tcaaagaggg catcatgaag
       61 caggagcagt acgactggtt ctgcaaggca accaattctc tttacggagc tacgccggaa
      121 cgtcaactgg cgtttgcctg caagaatacc ggttttttcg ctatggcgtt gatgctcgct
      181 gccaaagatg ccggccttga ttctcacccg atggacggat tcgatattga cggcgttcac
      241 aaagcgttta atatccccga ccagtactgg attccctgct tattggccat aggccatttc
      301 gacaaaacaa aaacccttct tccgccaaaa tggagaaaga gttatgaaga aatcgtgtac
      361 tgaaaaataa ccaattgcca tttttataaa atccggttaa ataagaatta ctaaattcaa
      421 ggagaagaag atggccgcta aaattaaaat ttacacgttg agcacatgca gtcactgcaa
      481 ggcaacaaag aaattcctga acgacaacag ccttcctttt gattgcgtgg atgtcgattt
      541 gctgcagggc gatgcccgtc agaaaatgct ggaagaagtt atgcagtaca atcccgaccg
      601 ttctttcccg accattctga ttggtgacaa ggtcatcatc ggtttcaagg aaaacgctat
      661 taaggaggct ttgggtattt aatgactcct gctgaactct acgacatgat gaaaaaggtc
      721 caggaaccga aaggttactt tttcaataaa gataaagatt gggttttaag catcctcggc
      781 gatcttttag tcaacaaaga acgttatggc tatatgtgtt gtccctgccg tctggcggct
      841 ggcaaccggg aaaaggacgc cgatattatc tgtccctgcc aataccggcc ggacgatgtc
      901 agggaatacg gcagttgttt ctgtaattta tacgtttcag ccgaatggaa tgaaggaaaa
      961 atcgaacatc agtacgttcc ggaaagaagg cccaaggaaa aaatcggctt ttaagtcctt
     1021 gtcaattcaa tgattttcaa attctgtcgg aaggccgggc aatttttttc atcttttctc
     1081 ttgatttccg taaataaatt attatattca agcaaaactt aatcattaag gagttttctc
     1141 catgataaat atgtataagt gcaagaaaaa gaacactctg atcagtgaag tctgcaccga
     1201 tacaacctgc gaatggcgtc tgaaaaacga atcctttcta aattgcacat gggtcgcctg
     1261 caattacggt ccgtttacgc tggaagaagt cggcgacatg atgggcgtta cccgcgagcg
     1321 aattcgtcag attgaagcca aagctctgaa gaaactccaa cataaaaaga gacgtgatca
     1381 actcaaagat tttgccgcac cgggtaacga ctgggacagc ctgtaactct atctcgcctt
     1441 cacagtcaat attctatacc tgccagtttc tcttcggaga tatttttgtg acacatccgt
     1501 tcgggaaaaa ttaaaccaaa aaattgtatg ctattcaggg gaattgattt tcaggtctga
     1561 tctatgggcc ttgatttttt gcggtgtact tttatgagca ttatgattgt tccgttatga
     1621 ctcgcggatt tctgttcgcg ccgctccaga atctgttttc tctattttct tcctgattta
     1681 tctttggaaa gcacttctct catttgctct tttcgctcat cgcgtgtaaa gagcagagcg
     1741 cagattgacg attcaatatc catggccgcc attttatcca tgcggggaac gatggagcac
     1801 agatttttca attcctcaaa agctctcgga gaacgttgtg acaaagtatc cgcgatactc
     1861 tccgccactt ccatgaatct ttccggcggt tccactttat tgaccagtcc cagtttcata
     1921 gcttcattgg cgccgatcgg ttctcccagc ataacgattt ccttcgcctt ggtgatgccg
     1981 acaatctgac tcagcgggta aaaaaacgga ttaaatccga atttcaattc cggatgacag
     2041 aagatggcgc tttcggatgc gacgacaaga tcgcaaacgg taaccagatt aaaacccccg
     2101 cccagagcga tcccgcccac ggcagcaatc accggttttt taaacgcata tatctttttc
     2161 aagtacatca tcatggactt caggaattta tcgctttctt tatcgggcag gttgttcatt
     2221 tccttgatat ccatacctgc cgaaaagaca tactctccgc cggtcagcac gactgcccgc
     2281 acatcggaat cggtttccag atcggcaaat acatcataca attcctgatg catctctttg
     2341 ctgatcgcat tcatttcatg agggcgattc aacttaatca cggcgtattt ctttttcttt
     2401 tcgacaatca aactatggta agtcattttt tattatcaac catgatacga agttcttttc
     2461 ctgttttgaa aaacggaact tttctttctg tcagtttcac ctgagtgcct gttttgggat
     2521 tgcggcccat gcgcggtttt ctttcccgga cctcgaaact gccgaacccc ctcaactcaa
     2581 tgcgttcgcc gcgttttatc gcgtcaatca tcgaatcaaa aatgatgttg acaacgtacg
     2641 ttacatcctt gagggaatac gttttgagtt cttcctgtaa ttttttgatc aaatcgtttt
     2701 ttgtcatgat aaactttcct ttatcttccg ggataatata agtaacttaa accgctattc
     2761 atctccattt tttccttgag cgacgcgctt attttactcg ccgcaccttc cagaacataa
     2821 tcgaaaatcg acgctctttt ctttgaagga taaaccagat cgaattcgcc gttgccccct
     2881 gctaatttat ccgcgagctt tgcggcggaa gacatgtcgc ccaaataatc aatcaatccc
     2941 aattccttcg ctttacggcc tgaaaaaacc cgtccatcgg cgatttcgat cactttttcc
     3001 cgggacagtt ttctttcttt cacaatcacg tccacgaact gattgtaaat atcgtccacc
     3061 agatcctgaa cgatgacttt ttcttcaggc gtcatgtccc gtaacggaga gccgatatcc
     3121 ttgtattcgc cgcttttcac cacggcggat tttacgccga ctttcttgag cagctcttcg
     3181 aaattggcaa actgcatgat agccgaaata ctgccggtta tggtgccggg attggcgaca
     3241 attttatcgg ctgcgcacgc gataagcaac ccgccggacg ctgcaatcga tcccatggaa
     3301 acgacaactt tctttgtttt tttgagctcg aggatggcat cgtatatttc ctgagaagcg
     3361 gcaacgcctc ctcccggcga atccaccctg agaaccaccg cagtaataga gccgtctttg
     3421 ccaaatttat ccaactgttc gacaatttcc agcgaatcgg caatcatgcc ttcaacaggc
     3481 acaacgccga ttttgttttt taacgaaaac cgggatgtct ttcccatgtt aaaaccggcg
     3541 ttgtaaaaag acagatagaa gatcagacca aacacaaaaa ggatcaacat tccaaacaac
     3601 acgggatgtt ttcgcatggt ttactcgccc ttttgtttcg tattaatttt tacgtcagca
     3661 agagcttggc tgagattgga accgacattt tccgtattat taatatactg attggatgac
     3721 ggggtcggcg ccggtgcttc cagctctttg atgctcaatc tgattttttt cgatttcgga
     3781 tcgatactct tcacgacagc ggaaaccgta tcgcctaccg aataaagttc cgaagatgtc
     3841 ttgacgcgct tctgactgat ttcagaaata tgcaccaatc cttcgatgcc ttcctcaatt
     3901 tccataaaaa tgccgaaatc ggtaaagttg gttatcttgc cggtcacaac actgcccgga
     3961 gcatatttct gggccagttc atcccatgga ttcttctcga tctgcttgac gccgagagag
     4021 aacttttcat tctgcacgtc aatattcaag acgactgcct caacttcctg gccttttttg
     4081 aaatattcgg aggggtgatt aacccggtgt ttccaggaaa tatccgacac gtgaatcaga
     4141 ccgtcaattt tttcttcaat tccgacaaac acgccgaaat tcgtgatatt tctgacaatg
     4201 ccttttacga ttgtgccaac cggatatttt tctttgagct tttcccaggg attcggcgtc
     4261 gtctgtttca ggctcaggga aattctcttg ttctgagcat tgacctccag caccttgacg
     4321 tcgatttctt ctccgatatt gagcacttta gacggatgtt ttatttctct tgtccagtac
     4381 atctccgaga tatgaaccag accttcaaca ccggatgcca gttccacaaa aacaccgtaa
     4441 tcggtcaaat tgacaacctt gcctcttaca atagagttca ccggataggt ttccgttatt
     4501 ttttcccagg gatttgccgt caactgcttc aggcccagag ataccctttc cttctccgca
     4561 tcaaaggaaa gaactttggt cgtaatttta tcgcctttct gaaacatttc cgacggcttg
     4621 acaattctcc cccaagaaat atcggtcacg tgcagcagtc cgtcaacacc gcccagatcg
     4681 atgaaaacac cgtaatccgt aatattcttg atgatccctt caacaacgtt gccttcctgg
     4741 atgtttttca gaatatcctg cttttccgct tctctttccg aagccacaat ggagcgtcgg
     4801 gacaaaacca cattgttgcg ctttctgtcg aacttcagca ctttaaaatc cagcgtctga
     4861 cctacgaatt tatccagatc ctttaccgga cggatatcca cctgcgatcc tggaagaaaa
     4921 gcgataatgc cgatatcaac ggacaatcct cccttgacct tttccacgac aacgccttcc
     4981 acgacggtat cattttcgca ggcattttta atttcatccc atatttttac ttttgacgcc
     5041 ttgtcacggg acaaaaccag atttccttcc gagtctcttc tgtcaataaa cacatcgatt
     5101 tcatcgtcga tggaaatggt tacgttgccc tgcgcgtctc tgacttcgtc gatcggaata
     5161 aaaccttccg ttttccagcc gacatcgacc atgatccgat cgtcttttat ctgcacaact
     5221 tttccggtgg ctatatcacc gtactgaaat tgattaaggc tttgttcgta aagctcttta
     5281 aaatccattt cttctttacc ggaagttata ttgttcttcg gtaaggattt ttcacctgct
     5341 tcatttttat ttgataagtt tgagttgtta ttgttaacca ttaaaccaat accccctgtt
     5401 ttttatgaaa cccagtaatt tttaaggttt aacataccaa atattaaata tcaacataaa
     5461 tcatgcaagt aaaatttatt taaataccag gctacttttt tgaaatcatg gacataattt
     5521 tttcggcaac ctcctcaatc gacaaattat cggaatcgac gatgatcgac ccttcgggcg
     5581 gcttcaacgg cgcaatggcc ctctcctgat cctgtttgtc tctgatcgac atatcgtttt
     5641 gaattgactg atattctgcc gtcgaacctt tgagtaagag ttccttatgc cgtctttttg
     5701 ttctttcggc aagtgtcgca tccaaataaa atttataatc ggcatcctgg aaaaccaccg
     5761 aacccatatc tcttccttcg gcgacaagac ctccccgcaa tccggcttcc cgctgcaggt
     5821 caagcagttt ttcgcgaaca accacaaagg tggatatttt agaggcaatc agaccgactt
     5881 cttccgtccg gattctgttt tcaacatcct caccgtccac gtaaaccctc atccggccgt
     5941 tgacattttt caaatcgact ttcgtggttg aacataattc ggacagctct gcgctgctgt
     6001 caatggagat tcggattttt aaggccttat aggccagacc tctgtaaaga gcccctgtat
     6061 cgaggtaaat ataacccagt cgctttgcca ggatcttgct gatcgtgctt ttgcccgctc
     6121 cggcaggacc atctatggtg atcacagctt ttttcttcat cacgtataat cctttttgtt
     6181 atttttaaat gaaaacttgt ctttaaaatc caaagaatgt aacattgctt gaaatgtgaa
     6241 atcggaaaat gcttatgtgc aaattcattc aaaatcaata tatttatttg ggcatgattg
     6301 tattgatgct gctgttgacg tcggacattt gtcgtgctga atttaccatt gaggatgaaa
     6361 acaaactggg cagggaattc tatgagcaac tagaacagca tcagatgatt ctccataata
     6421 aagtgctcac agattatatc accggtatcg gcaatctgat tctggatcaa accacccgga
     6481 cacctttcaa atttaatttt tttatcctcg acagttccgc catcaatgcg ttcgccactc
     6541 ccggaggtta tatttacatc aataaaggat tgctgctggt tgcggagaat gaagcccagt
     6601 tggccggtgt catcgcgcac gaaatcggtc acgccaatgc gcgtcatgtc gccagcatta
     6661 tttccaaatc tcagaaatta aatatggcgg cgctggctgc catgctggcc ggtgttttcc
     6721 tcggcggagg aggagaagca tcggccgcga ttaccgcatt ttctctggcc gggtccagca
     6781 gcatgacgtt gcgctatatg cgtgagcatg aagaagaagc cgaccgcctg ggaatagatt
     6841 atctcgtcca cgcaggatat aatccgtcgg cgatggtcga ttttctgaaa atcatgaaac
     6901 agtacgaatt tctctccaag accatgccat cttatctaca gacacatccc ggaaccgatg
     6961 accgtatcat ttacatggat ggtttgatta tgactcgata ccggcagcca gggaaaaaca
     7021 gcatcatcgg caacctgtgt cgggtccagg caagcgttcc tttaagcgcc tccgaactgt
     7081 caagacggca aagacaactg gagacgtctt tagccaaaga cccgcaaaat accgacctgc
     7141 tctacaacct ggccatgatc gaagatcagt tgggagccaa cagctctgct cttgagcatc
     7201 ttcagaaagc cctcgttcaa tcgcctgacg atcacgagat tctgaaaagc atcggcctgc
     7261 ttcatctcaa aaccggccga tatgaacagg cgcagaacta tctcctgcgt gcaaaaaaat
     7321 cagacccccg caatgatgaa attacctttg cgctcgggaa aacgtatttc tcgtccggaa
     7381 aataccggga agcgctggat tgttttttga atatccggga cagggcgtct aatgaggcgg
     7441 ccaatctaaa ctactttacg gccatgagct acggcaaact ccaaaataaa ggggaatcgc
     7501 attattattt cggtttgcat ttcaagcagg ccaacaaaaa agacagcgcc ctttttcatt
     7561 tcagagaagc cctgaatttt ttccccccgg gttcggaacg aaacgccgaa attcaccggg
     7621 ccgtcaaaga actgacggca ggcaaagaca agcaaaaaag aacaataaaa aaagatgaac
     7681 aaaattcgtt tctatgaccg gatcgaactt gacttcgtca gaagtctgca tattttaacg
     7741 aaaaagagga ttccataaaa tgcccatata tgaatttcga tgcaaaaagt gcaaacatat
     7801 ctttgaaagc ctgattttct ctcctaaaga agaaaaaggc atgatctgtc ccaaatgcaa
     7861 agcgaaaaat gcggaaaaaa tcatgtcggt ttttgcgggc ggaaaatccg gctgctcatc
     7921 gtgcacatca acgtcatgtt ccggttgttc ttcctgcggc ggccattgag tcgtccgtat
     7981 ccggttttgg cgttttttcc ggttctttct tttattattg ctaaaatgct tacaatacat
     8041 tataagactt tcactttaat atttaccatg aaaggagtct cacaacgtga acattttaat
     8101 ttttggcccc aacggcagcg gcaaaggaac tcagggcgca atcgtgcaga agaagtacgg
     8161 cgtgccccat attgaaaccg gcgtcatttt ccggcagaat atttcgaaga aaacgccgct
     8221 gggagaaaaa gcaaaggctt ttattgaccg cggcgaactc gtccctgatg atattaccat
     8281 ccccatgatt ctggaccgtc tcaaggaaga cgattgcaaa aaaggatggc ttctggacgg
     8341 cttcccccgc aatctcgctc aggctgaagc gctctgggat gctctgcaga aagaaaacat
     8401 caaactggat tccgtcatcg aaatcgttct ggaccgccag attgccaaga accgcatcat
     8461 gggacgcaga ctttgcaaaa tcgacaacaa ccatcccaac aacattttca ttgatgccat
     8521 caaaccgcat gagcatgacg gcaaaaccgt atgccgggtc tgcggttgcg aagatctgtc
     8581 cacccgcgcc gacgatcagg atacagccgc catcgacaaa cgtcacggca tctactacga
     8641 caccaagacc ggcacactgg ccgctgtgaa tttctacaag gcaaaagtga aagttattga
     8701 agttgacggt agcgttggcg tgaaagaagt tacagatgaa ctgatgaaga aactcggata
     8761 aaaacttaag acacgtgaca aaaaaagccc cccagcattc cttggagggc tttttttaaa
     8821 aatactttat ggaaaacata agaaatttca gcattatcgc ccatattgat cacggcaaat
     8881 caacgcttgc cgaccgtctg attcaattta ccggcgtctg caatgaaaga aatttccagg
     8941 atcagattct ggacaatatg gatatcgaac gggaacgggg cattaccatt aaaagcaatg
     9001 ccatctccct gccctatcgc gccaaagatg gaaaaacgta catcctcaat ctgatcgata
     9061 caccgggaca tgttgatttt tcatatgagg tttcccgatc gctggcatcc tgtgaaggcg
     9121 ttttgcttct gatcgatgcc gcccagggcg tgcaggcgca aacactggcg aatctttaca
     9181 tggcgctgga acatgatctg gaaatcattc ccgtcatcaa taaaatcgat ctgccctctg
     9241 ccgacattga ccgcgtactg gaagaaattg attcggaatt ggggcttgat cccttcacgc
     9301 atatcaaatg ctccgccaag gaaggcgccg ggattgaaga agttctcgaa gcgattgtcc
     9361 tgcgcatccc gccgcccaag ggaagccccg caaatcctct ggccgctctg attttcgacg
     9421 ccaaatacga ttccttccgc ggcacaatta tccactgccg cgtctttgaa ggaaccatca
     9481 aaagcggcga catcatcaaa tttatgtcca acggcgccac ttacaaagtg gaggaagtgg
     9541 gacattttct gctgaaccgc accaaacgcg aaaaactttc cgccggtgac gtcggctaca
     9601 tcatcgccgg cgtcaaaacc gtttctgatg tgcgcaccgg tgacaccatc acgcttcaga
     9661 acaggccatg cgttcagcct ttgcccggtt tcaaggacgt caaaccggtt gtttttgctt
     9721 ccatctatcc catcgcgtcc gatgattatc aggatctggc cgactcgctc gaaaaataca
     9781 agctcaatga tgcatctttt atttatgaaa aagattcttc ggccgccctg ggacagggct
     9841 tccgttgtgg atttttaggc ctgcttcatc ttgatatcgt acaggaaaga ctggaacggg
     9901 aatttaacct ctccattatt ctttccgtac ccagcgtacg ctaccgcttt tcgctcacag
     9961 acaaaaccgt catttacgtg gacaatcccg cgcattatcc cgatccgatc gaaatcgaca
    10021 aaggtgagga gccttacatt cgctcctcca tgatgcttcc ggaacgctac ctgggagcgg
    10081 tcatgaaact gtgtatggaa aaacgcggcg tgaactctgt catgaattat actggtcccg
    10141 gtcgtgtgga attatcctac gagatgccgc ttgccgaagt gatttatgat ttttatgacc
    10201 gcttcaaatc cgtcacccag ggctatggct cttttgatta cgacatcatc gattaccgcg
    10261 agagcaatct ggttttaatg gacatcctgg tcaacggtga aaaagtcgat gcgctctccc
    10321 agatcgctca tcgagatcgg gcgcgcgcaa gagctctgct ggcctgtaaa agactgaagg
    10381 aggagattcc tcgccagatg ttcaagatcg ccattcaggg tgccatcggc ggtgaaatca
    10441 ttgcgcgcac caccatcagc gcctttcgca aggatgtgac agccaaatgc tatggcggcg
    10501 acattacgcg caaacgaaaa ctgctcgaaa aacaaaaagc gggaaaaaaa cgtatgaaga
    10561 tgatcggttc ggtcagcata ccgcagagtg catttctggc agttctgaaa tccgataccg
    10621 actagatctt gacaccaacc gatcttttct ttgattgatt cttatgcgaa gcgaaaaagc
    10681 cggcctttac attcacgttc ctttttgcct gagcaagtgc gcctattgcg atttctattc
    10741 cgtcaaaacg cttcacctga ttcctgacta tatcgcggcc gtccaaaagg aaatgaaact
    10801 gtatcgcaac gtcttttctt cattcgattc catctatatc ggcggcggca cgccatccct
    10861 gctggccccg caatacctgg atgaaatatt cacagcatta aacaaatatt ttatcattga
    10921 taaaaacgct gaaatcacac tggaggccaa tccgggagat ctttccccgg tatatttgaa
    10981 agcgctgcgc gacacaggga ttaatcgtct gaatatcggg gttcaatctt ttaacgataa
    11041 gattttaaaa tgccttggcc gacggcattc ggctcaggaa gcgatggcgt cgataaacgc
    11101 cgccagaaaa gccggattca gaaatctggg tatcgacctg atttacggcg tttacggact
    11161 gggtcttcaa ggatgggaaa aaattctgca acaggcgttg atactggcgc ctgagcattt
    11221 ctcctgctac cagctttccc tccacgaaaa aacaccgctt cacaaaacat atctttccgc
    11281 cggatggcat cttccggatg acaatgcgga actgaaattt tttttaacga catccgaaat
    11341 tttggaaaat gtcggttacc ggcattacga agtctccaac tttgcccgaa aagacaaata
    11401 tacatcccgt cataatcaga aatactggca gcacgttccc tatcttggtc tgggaccggc
    11461 cgcgaactct tttctgaata atcaacgatg gtggaacaag gcatctgtta aacgttattt
    11521 aaacgccatg gcccgcggca agacgcccgt tgaagcatca gaaacactta ccgtcaatca
    11581 actgcggctg gaagctctgt ttctggggct acgctgcaaa tccggcatcg atctaaagcg
    11641 cttcaaagat caatacggca ccgatttgat gactgaaaaa aaatctatta tggatgcttt
    11701 aatgaaaaac aagctcatag agttaaaaaa cggttttctt tgcccgacgc gttccggaat
    11761 ggctcttgcc gacagcctgg ctttaatctg agtggcagct gatacagaac ttgacatatc
    11821 ggctacaagt acctgtttta cattacgtat ataaatagac agacaggaag tgtctgtaag
    11881 ataatgtatc accggatggt tacagaagga atctgctgct caagttgttt tttaattttt
    11941 cgcagtttct cttccgaaaa agatttttcc ttcgcgaact ttttatccag tgtcttcttc
    12001 gccataaaat tctgcagcac gtaatgaccg gcgccgttga tcatttttcc gatctgcaag
    12061 atatcgtttt catcgagctg agattccaca atcgttgtgc ggaattcata gggtatcttt
    12121 gcgtttaaaa ccagccggat gctttcctga atagcatcct gattcacatt ggccttgaca
    12181 atgtccctgt acttgtctag aggcgccttg acatccatgg cgatgaaatc cagcatgttg
    12241 tcggcaatga gggttttgag aacattcggc acagagccgt tggtatccag tttaaccgca
    12301 aatcctattt ttttgatctg cctgatgaaa gagatcacat tatcctgaat ggtcggttcg
    12361 cccccgcaaa tcgatactgc gtcaagtttt ccttttcgcg attcaagaaa atcaatcacc
    12421 tcactgactt tgataggtgt gcgaaataaa tcgggattga ccaattcggg attatggcaa
    12481 tagggacatt tgaaattaca tccctgggtg aatataattg cggaaatata ccccggataa
    12541 tcaataaggg acactctctg caaaccgccg atcttcatca tacgcctctc atagattaaa
    12601 aaaacaaacg cgccgccgcg tctgatgatt ctagactctc atgatccgtt tatttgaatt
    12661 tgaattcctg gcgcaagtca aactcttcct gcttgccttt attccactgc ttgaccggcc
    12721 ttaagtaacc gacgacgcgg gaatagactt cacaatcatc cgcgcaggtg ggacacttct
    12781 cctgttcgcc tttaatgtat ccatgagacg gacagacgct gaatgtggga gtgaaggtga
    12841 tgtacggcag atgataattg aaacaaatct tctgaaccag tttcttgacg gattgaggat
    12901 cgtcaattct ttcgccggca aatatgtgaa acaccgtgcc gccggtatat ttgacctgaa
    12961 tttcatcctg caaatccagc gcttcaaaga tatcgtcggt ataatccacc ggcaactggg
    13021 tggaattggt gtaaaacggt tccgcctttt tcgatttgcc ttcgctggcc gtaataatat
    13081 ccgtgaactt ctctttatca attttcgcca accggtacga cgtaccctct gccggcgtcg
    13141 attccagatt ataattatta ccggtttctt tctggaaact gatcaatttg tttcgcatga
    13201 aatccaaaac ctgcttggtg aatacctgtc ccttatccgt cgcaacgttc acgcccagca
    13261 gattcagaca agcctcgttc atgccgacca gtccgatcgt ggagaaatga ttcttccagt
    13321 actcgccgaa ccgttctttc actccgcgca gataatactt cgtataggga taaagatttc
    13381 cgtacgtgaa cttctccagc acctttctct tggtctccag actttccttc gccagttcca
    13441 tcagatgctc cagacgcgtc aaaaaatcct ttttggattt tgaaagataa gcaatccgcg
    13501 gcatgtttat cgtaatgaca ccgatagatc cggtcagagg attggagccg aacaaaccgc
    13561 cgcctctcat ttccagctcg cggttgtcga ttctcaggcg gcagcacatg ctgcgggcgt
    13621 cttcaggatt catgtccgaa ttaataaaat tggaaaaata gggaacgccg tacttggctg
    13681 tcatttccca gagatcctgc aagttgggat cgtcccagtt aaagtccttg gtaacgttgt
    13741 aggtgggaat tggaaatgta aacaccctcc ccttggcgtc gccttccgcc atcacctgca
    13801 aaaaagcctt attgaaaaga ttcatttctt cctgaaattc tctgtaggtc tctttctgcg
    13861 gctttccgcc tatgattaca ttctggtcgg cgtagtattt cggcactgtt acatccaggg
    13921 tgacatttgt aaaaggcgtt tgaaatccaa cccttgtcgg cacattaata ttgaaaataa
    13981 attcctgaag ggcctgtttt atttctttca tagacaaatt atcgtagcgg atgaatggag
    14041 caagcaaggt atcaaaattg gaaaaggcct gagcgccggc ggcttcgccc tgcaacgtat
    14101 aaaagaaatt caccacctgt cccagcgcgc tgcgcaagtg attcgccggt ttgctctcca
    14161 cttttccgga cacgccttga aatccctgca gcagcagatc ataaagatcc caccccacgc
    14221 aataaacgga cagcagactc aaatcgtgaa tgtgaaaatc gccttccgta tgcgctttgt
    14281 gaatttccgg cgtgtaaatt tcattgagcc agtaaacctt gctgacttcc gaagaaatat
    14341 aattattcaa tccctgcagg gagtaatcca tgttgctgtt ttcattgatc ttccagtcaa
    14401 gcttcttcag gtattgatcg acgagatcaa cttccattcg attggcgata tctctcaaac
    14461 gggagtgctg atctcgataa atgatgtacg cctttgctgt cttgcggtac ggagagctca
    14521 gcagaacttc ttcaacaaca tcctgaattt cttctacgga caggattttg ctgtcgaaga
    14581 gtttctcggc cagattcagg actttgatcg ttaatttacg cgcctctttg atgtcaaaat
    14641 cgcctgtggc atttccggcc ttggcaatgg cattggtaat tttttccgcg ttaaatttaa
    14701 ccagtcgccc gtctcttttt ctaattttaa gattcataaa aacactccat ccgatgaaaa
    14761 aaataatata agaataaatc caaaatagcg ctatatattg gggttgaaaa ggatagtacc
    14821 actatatata gtaattttca agggaataat aaataatttt taaagtggct attccgatgg
    14881 aaactcaaag agataggctg attttagtgc aaaaaaaata cgaattacgc ttaaatcttg
    14941 ttgtattcgg aaaagatcat ggagaaaacg gccctcccct gtgtcgagga cctcaaatcg
    15001 gtggaatacc cgaacatggc cttcaaggga accttcgcct taatttcgct gaccgttcct
    15061 ttgggattaa cggcctgaat ttcacccttc cgggcattaa tgtcgccgat cacatctccc
    15121 atgaactcgc tgggcgttat cacatcgacc atcataatcg gttcaagcat gatgggctcc
    15181 gcctgaccgc agccgtcgcg aaatgcggtt gaagcggcta ttttataccc ctgcgctgaa
    15241 gattcgccct cacggaaaga accgccgata atcgcgactt ccgtatccat gacagggtaa
    15301 ccatttaaca cgccgttcat cattgcttcc cggatgccct cctcgatgac ttcatggaaa
    15361 tcctgaggaa tgacgctatc gtcaagtttt atgatcacgt catttcccga acctctcttg
    15421 cgcggggaca gggcaatccg gacgtgaccg aaatgcttct tttcgccaag ttctttttca
    15481 aatatacctt cgatttccac tttcttctgg atggtttcgc ggtagacaac cctgggccgg
    15541 cccacattca catggcagtt gaattcccgc agcaaacggt caacaataat ttccagatgc
    15601 agttccccca tgccggaaat gatcgtctgg gcggtttcat cttcgtattt caccttcaac
    15661 gtcggatcct cttccaccag cttatccagc gccgccgtca gtttttcctg atcggctggt
    15721 gttttggctt caatggcctg actgatgacc ggttcataaa attcaatcag ttccagtaaa
    15781 atcggcttct gctccgcgca aagcgtatcg ccggtggtag tggatttcaa tcccagaacg
    15841 gcaatgatat ctcctgcgga ggccgagtct atcctttcac gtttgttggc gtgcatgcgt
    15901 aaaaggcggg ctatcttttc ccgccgtttt tttatcggat tatagatttc gttattgacc
    15961 tgaatttttc cggaataaat tctcagatag gtgagctttc ttccctcgtc catcataatt
    16021 ttgaacgcga gcgctgccaa agggtccttc tcggaacttc gtctgatctc ttcttctttc
    16081 gttaccggat tcattccttt gacgggcgga atatcttcag gagacggcag atagttgata
    16141 atggcatcca gaacgggttg tatccctttg ttgcgcaggg ctgagccgca caagaccgga
    16201 acaattttca gagatatcgt cgcttttctc agcgcctctt ctatctcagc aatggtaatt
    16261 tcttcaccgc ccaggtactt atcggcaagg ctgtcgtcaa cctcggcaag cgtttcgatc
    16321 attttatcgc gctgcaactt cacgtgatcg gcaaattccg acggcacgtc ctgcttagac
    16381 atttccgacc cggatgtcga atcgttccag acgatcattt tctgattaat cagatccagc
    16441 acgccacgaa agttatcttc actgccggcg ggaatttgaa tggcgacggg cagagaggaa
    16501 aaacgttccc gcatcattcc aatcacatta aaataatcgg ctcccactcg gtccattttg
    16561 ttaataaacg cgattttcgg aacaccgtat ttgtcagcct ggtgccagac ggtttcggat
    16621 tgcggttcga caccgcccac cgcgcagaag acgaccacgg ccccgtccag cacgcgcaat
    16681 gaacgttcca cttctatggt aaaatccaca tgccccggtg tatcgatgac atgaatgtca
    16741 tgattgcgcc agacacaggt cgtcacggcc gatgttatgg tgatgcctct ttcctgctcc
    16801 tgcggcatcc agtccattac cgcttcaccg tcatgcacct cgcccatctt gtaggatttg
    16861 ccggtatagt aaaggattct ttccgtaacc gttgtctttc cggcgtcaat gtgtgcgaca
    16921 ataccgatat tgcgcgtccg ggctattttt gatttggatg ccatatgcgt cgattaattg
    16981 ttaggggatt gctccgggat aatgagttcg agatccggcg aaaaaaactt gcctgttgaa
    17041 atgtggccct gaatcgagga aagctcctta ccgtcagcca gaatttttac tcttttgatt
    17101 tccggaatat tctgcgtgat ggaattcgtt aacgaataaa tcgtattcat ctccgcggca
    17161 gaactgcctt gatacgcctt gatcagatct ttgctgaaat taacatacgc aacatcatga
    17221 tccttaattt tgacatcccg gagcgaaacg tttttgggaa aggtattcac cagtcccgtc
    17281 ttggaaccgt caagcagagc cttgacgatt tcctgagcct gcaacgcaat gtcgctttcc
    17341 ttgatgacat ggcgcttttc agccttcaaa aaacgctcct gctgatccga aaaataaatt
    17401 ttaacaaccc gcctttcctt tttggtcatg acgtccgtat cgacgggggg gaacagtgcg
    17461 ctgaatatgg taataaaaaa aatgacgagc aagactacac cgccgccaac aatcagcgaa
    17521 aggtaaaata cccgggttga tttttttatc ttcttttcct tgatgctctc tgaccttagc
    17581 tgtttcttgg tcgacataaa atactccatt cctcagaaaa acgctgccaa tctatggcat
    17641 tttatatgaa ctgtcaagaa gaacgagcgt tgataaaaaa aacagcccct gcattgagac
    17701 atcagaaacg caggaagctg ttttattctt acaactctat cgttctgttc gcctgaaaat
    17761 ccttaatggc tcatgatccc cttcctactt ggctgccggg atagtttcca attccgattt
    17821 atcagaattg gatagttcga cttacgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnngga tagttcgact tacgcttcgt gcttcgtttc actcgccctc agcttgtctc
    18001 actagtaatt ttcagccagc acttcataat aggcctgcgg gtgtttgcat gcaggacatt
    18061 cttttggtgc ttcttctcct tcgaaaacat aaccgcaatt gatgcaatgc cattttgtcg
    18121 ctgtcttctt tttaaagact tcgccctttg cgatattctc tgccagcttt cggtatctgt
    18181 tctcgtgaaa catctccact ttggcgatct gttcaaaaga acgggccacg tccggaaatc
    18241 cttcctctct ggccaccttg gccgcgtcgg aatacaaaac ggtccattcc atattttcac
    18301 cttccgccgc ggctttaaga ttactgatcg tatctccgat taccccggcc ggataagagg
    18361 ctgtgatctc aacttcaccg ccttcaagat atttgaagaa taccttggcg tgctcctttt
    18421 cattttccgc ggtttccaga aatattcttg aaatctgttc atacccttct ttttttgctg
    18481 cgctggcaaa aaaagtatag cgatttcttg cctgtgcctc accggcaaaa gaagccagta
    18541 aatttttttc cgtctttgtt cccttaatcg actttgccat gcgtcatctc cttttctttt
    18601 ttgatatgtt aaacactgtg attgaactta gcaatggtca tttcataatt tttttgccaa
    18661 tggaaaaggc ctctcccaga tatccgccga ctccatgata agaacgattt tccggagcat
    18721 atatcaaagg ctggattttc atgatatcgg gcaattgatc ctctgtcagc agggttgttt
    18781 cgactttaac gtcacatatt tctccgatca gatgcacatg aatcccgact tccatctgat
    18841 ccgtcaacct gcattcaacc accatgggac attccgcgat ataaggcgca tccacaatgt
    18901 tgctgtggac agccgtcatg cccgctgctg caaatttatc cgttttctta ccggacacca
    18961 tgccgcagta atccgccgct acaacctgat cggccgtggc aatatttacc gtaaacgcct
    19021 tgcggtgctg aatactttca taagtatatc gcgattttct cagtgaaatc gccaacgcgg
    19081 gcggatcaga actgcagaca ccgcaccagg caacggtcgc agcattcggc ctgccttctt
    19141 gatcatacgt tccgatcaac catacagggg tcggataaat caacgtttta gctccaaatg
    19201 actttttcac agcaatctcc tcaaaaagca atttagcgga ttttgttatt tttttcattc
    19261 attttgaaaa tataaatcaa aattccaaaa atttaaatta atttctgtgt tattttatag
    19321 tatatgaatt aaaaatgcat ggaatcatca ttttaaaaat gtgttttcca aatcagttta
    19381 ggagggtttt ttatggctac gatcatgccg gaagcggaga atgttcagaa agcaatcaaa
    19441 tggatttcag caagtttgga aagcgaaaat ctgcccctgc aaaaactcat tgaaaaggcg
    19501 attttcaaat tcgatctgtc tcccaaagac acagaatttt taacgaattt tttccgcgaa
    19561 agtcgttcga ataaataata tcatctctca aattcactca gataaatcac gctcgggcaa
    19621 ccgaagttgc ccgagccctt cgccgtaacg acgtgaaaat ctgtttgtgg agcgaccctg
    19681 ctttattttt tcagcagttc acgaatgtcc gtcagcaatt cctgatcctt ggttaatggt
    19741 gcaggtccgg cggcttcttc ctctttgctc ttcatcatca tacccagaaa tttcacaata
    19801 aaaaaataca gcgccacggc cacgatcaga aaatttacaa tttcgccgat gaacaacccg
    19861 taaggcactt ccttgccgtt gatgatccac ttccatccca gataaccctg ctctcccggt
    19921 attacaacac taaccagcgg catgataata tgcttcacca gcgaatcgac aattttaccg
    19981 aaggcagctc caatgatgac accgatggcc agatcgacaa catttccctt gaaagcaaaa
    20041 tttttaaact cctccatcat cgcggatact ttgttttttg ccgccatatt cccctcctct
    20101 gaatgtttta attgactctt gacatttcct cgtaaatttt caactcgatc acactgaaca
    20161 aattatcata ctgtggatgc tgcagaagcc tgcgcatttc ccaacctttt tccgcatgct
    20221 tttccgtaat cacagaaacg gttacctcgc attccttcca gttgatttgt cggactacag
    20281 cggcagcttt ggtcctccac tgtcccgaaa ccacataatc ggaatcgacc cgtttgtttt
    20341 catagtctat tttcgtgtcg gcgcccatct ttttttcact gaacacacgg gcaaccgctc
    20401 taagcgccgc tgtttctttc gtttcaaaag ttcgcgtgat ctgatccggt tttgaaatca
    20461 cggtgccgcc cggtccgagg ccgaaacagg agatgaggtt gaggaccgac aggacgagga
    20521 ggaaaaagga cacacgatgt tttttatgaa tcatagaatc gttttatgtg ccggcgatgt
    20581 tttcttcatg gattattacc gttcacccac cagtcgccct tttctttaaa ccaccacaga
    20641 gaagaatccg tcccggtaat tttttcagaa agttttttcc cgttttccgt gtttaatctc
    20701 tgcacggcaa aacctatcca ttcttccgcg tatttattgc ccagatcgcc gcattctttc
    20761 aactgtaaaa tcagggcaat ttgatcggca tccctggcga tcagggcttc ctttgttttt
    20821 ttctgattaa actcgtcgat tgccgatctg atatccgcgc cgaaagaaag actttccgcc
    20881 aattcgcgga ttgctttttc ctcatccacc ttgacgtact ttttattgac gtaattcata
    20941 tcgccggttc gggcttccgg cagatcgtga aaaatacaca ttttcaggac gcgcatctca
    21001 tcgagagacg aatccatctt gcaaagcgta tatcccacaa agaccgtgcg gagaatgtgt
    21061 tcggccaccg actcctgacc gctgcccaga aactgataac cgctgcgcgg cgttctgctg
    21121 agcatcccca cctcaaacaa gaaattggcg atactgtcca tgctattcct ttttcagcgt
    21181 tttgatccga ttggacatca tttccgatat ttttgttatc tgtaactgca tggacgccac
    21241 accgtcgggt ctcatggcaa tcactttgtc caactgggct ttccagtgtt ccagagaagc
    21301 aatctcgttt tctctgattt tcttctcgaa gcgatcctga tattctcgaa taaataaatc
    21361 cgttgaactc atttgcgcca ttttccatct ccttttaatc ggcaagttac ggcattatat
    21421 catcggacgt ttatcttgca accatgaatt tcaaaatcat aatttgacac cgggcggtaa
    21481 atgctataaa gaagacgcaa aaatttgcag tgaaggttat atgcccccca aaccacaacc
    21541 ggaagatatc gccggaggcc aggcgctgat cgaaggcgtc atgatgcgcc acggcgataa
    21601 gatagcggcg gccgtccgaa gaccggataa tgaaattgtt tttcaggagc gcgaatatat
    21661 tcccctgacc aaacgttata aagtcctcgg atggatgttt atccgcggaa ccatcacgtt
    21721 gtttgaaatg atgatcgtcg gtttcaaagc gctgatgttc tctgcggatg ttgccctttc
    21781 ggaagaggaa cgaaaaccca aagactggga aatgtatctt tctctgatga tcagtttttc
    21841 gattgccatt tttttctttg tggttgttcc ggcatattgc ttcactaaaa tgaagttcta
    21901 tatcgacaat cttctgcttt taaatattct tgagggatgt gtccgcctgg gaatgtttct
    21961 ttgttttctg gccgccactt tgctgatgaa ggatatgcgt cgcgtctaca tgtatcacgg
    22021 cgccgaacac aaaaccgtat tcgcgtggga aaacgggcaa gacctgaccg ttgaaaacat
    22081 caagaatttc tccacccgtc atccgcgctg cggaacaagt ttcattcttt ttgtgatgat
    22141 tgtttccatt ctggtttttt ccctgctggg acgtcccgat tttctgcacc gggtcctcta
    22201 caaaatcctg ctgcttcctg tcgtatccgg aatatcctat gaagcgattc gttttaccgg
    22261 gaaatacaaa cattccagac tggtgcagat cttaagctgg ccggggctca tgctgcagag
    22321 aattaccacc cgcgaacctt cggatgacca gatagaggtg gccattgccg ccatgaaaaa
    22381 agtgatttaa ctttttgttc aggtctcgtc agcccgaatg tatttttcgg tcagccatcc
    22441 agcttttttt cggaagaaac cgtccgcttt ctctcaaagc catagaataa gttgactttt
    22501 attgccaatt aaagtatttt catacaatat ttatacactg aatatctcaa ccagggggaa
    22561 ggaactgatg tttaagcggt acgttttggc tctgttgatc gttttagctt caacaacctt
    22621 ggcttggaac attgtcgaag cttcgcggga agatacggcg cagattcaac tgaagaaaac
    22681 cgccatttcc agaaaaaacc tgcacaccta cacggtaaag gaaggggatg ttttaatcaa
    22741 tatcatcaaa agcattcccg gcatttcaga aaaagacatc ccttacacgt atcgtctcat
    22801 caaggatatg aatccccaca tagcaaatat tgaaaacctc gaagtcggac aatccatctt
    22861 tcttccggaa aaaccaatga aaatgaaaaa cgaccggaca aatgtgaaag ctcctgcagc
    22921 cgctccgaaa accgtatctg ccggtgaatt ttattataaa gtaaaacgag gggacacctt
    22981 atacaagatc atccgccgcg aattcaaggt ctcggaaaca tccatcccgc aggtggtgcg
    23041 aagcattaaa gttttgaatc cgaccatcaa aaatgtcaac aaaatctatg tcggcaccat
    23101 gctaaaactc cccgggaaaa ccgtttttgt tcaaagccct gacgacctta cgccggcaac
    23161 ccgcaagccg gccataagcg acaccgccga acaatacgtt caggaagacc ggatcattga
    23221 gatcaaagac aagaaaatga tgccgtttga agcccgtctt gccttgctaa agcagatcat
    23281 cacgcagatg aatggaacgg tcacaacaac gggcaattat tatcttccca tcccccaggc
    23341 cggacaggtt accatcgatt gctccaagat tccgctcatt gaatttgacg acaacacgac
    23401 cgtttttctc gatttggaaa accgcgctca cagtaatttg aaaaagatga tttccaacaa
    23461 ctggaaaaat tatcatttgg tcaaaatcag caatactgat gataccatta ctattctgaa
    23521 aaaagttttc agtgtaacga acaagtacag cattctcaaa agcgacaagc ctctaacctt
    23581 cggcgccttg ccgcccgcgg aagtcatggt ggattggttg atcgtcaaac ccatcccagg
    23641 acagcaatcg aaatcagtca tccagggact gcggcagatt tacgaaaata atgttctgct
    23701 gcccaaatcc attaaaaatt atgcccagaa aaacggctta atcataaccg agttctccga
    23761 ggaaacaggc attgcggata agcccgagga actttattcc ctgccgccga ttcccgtctt
    23821 tccggcgacc aatgccaagg aattttccca cgcgcttgtt tcccacctgg gactcagtgc
    23881 agaaaaagat gtcgaaattc aaatctttga cacggtaaag gacggattta atctttcctt
    23941 aaaagcggac gttctcgtca ccaagggaaa caataaatat gtcatatatt cccagtctct
    24001 ctctcaacaa tttataaacg ccctgaaaca ggccggcaat gaaaccatct ttgtaagcaa
    24061 cagcgattcc accaaagaca tcatgggcaa cgtcttgagc ggcctggata ttccttttac
    24121 ttacggcacg cacgcatttt ccgggctgga aaaaaatcag gctccttatg ctctgaaatt
    24181 cagcggcacc agaatcaatc cggatttata tgtcgtcaat tttgatatcg atccgggact
    24241 gcgcggatta ctccaggaag tctggtcggc caggattgcc cggtattaat cccctgctcg
    24301 aaaaatcgga catgcccgta aatcattgaa gcttttaaaa catctgcttc gaagctgcgg
    24361 ttgcttcgtc gatccgctca accggcaaac gctcgacgca tcaattttgt tcttgttttt
    24421 cttctcaaaa ttgtttttca tgacagcggc agctaaacaa ccggtaatgt attgttaaag
    24481 gattggaatt ttatgaccaa agcgattgct cttttttccg gcggcctcga cagcatcctg
    24541 gccgcggaat tagtccgcag acaaaccatc gacgttctct gtctgaccct caccacaccg
    24601 tttttcaatg cacaaaaagc gctggcggca agccgccagt tcaatctgcc gctggaagtc
    24661 atggatatca cgtctgagca tttaaaaatg ctccggtcgc cccgttacgg ttacggcaaa
    24721 aacatgaatc cctgcatcga ctgccacacg ttgatgctga acattgccgg acggaaaatg
    24781 gatcaaaccg ggagcgattt cattgttacc ggagaagttc tgggacagcg cccgatgtcg
    24841 cagaataagc aatccctgaa tattgtcgcc aaaaattcag ggtaccggga ctttatttta
    24901 cgtcccttaa gcgctcgtct tctggaaccg acaaaagcag aaaaagaaaa tctgatcgac
    24961 cgctcacagt tacttgccat tcagggacgc ggccgaaaac agcaaatcat cctggccgcg
    25021 gatttcggca tcacaagtta tgctcctccg gccggcggat gcctgctgac cgatcccgtg
    25081 tttacccgtc gcctgcgcga tttgtttgcc catcagaccg aaccggaaat ccgtgattat
    25141 gaattactga aacacggccg tcatttccgc attcatgata catgcaaaat cattgtcgga
    25201 cgaaacagcg gcgacaatga ggctttgaaa catcttgccg cctccggcga cgtcctgttc
    25261 aatatggctc attttcccgg acccctcgtt gttgttcccc gggacagcct atgcgatcca
    25321 cagatcgccg ctgcgttatg tgtgcattac agcgatgcgc cgccggatgc cctgcaggag
    25381 gtcatctgcg tccgggacaa ccagtcggaa atcgtcacgg ctgccgcagc atcaaaagaa
    25441 gattgtcagc gatggattct ttaaacgtgt aggatccgaa acacattcta tcggaccgaa
    25501 tgcccgggac gcaaaagatc ataaaccgtt ttgaccggat taaaaacaac gatcggtaat
    25561 ttcacaaaca ccgtattcca gtacgccatg gaaccgttcc ataaaccggg cagttccaac
    25621 gccttgagtt ggcgtccttt ctcgcttttc gacgtaatca gatacgtatc gttgttgaca
    25681 tagtcggaga gatcgaaaac cctcccccgg taatcccgga tgcagcagac catatcaacc
    25741 ggattgaaat attccgcgcc aaaccagatg gcagcctgat ccgtctgttt cgtatccaca
    25801 tgcgcgcttt caacaatctg aagggtctgc gtcccgtctt tttcttccac ccagaaagga
    25861 ccaccgcccg gctgattttc gtttcggacc atcccgcaaa cccgtagcgg tttgttcaaa
    25921 caagaaagaa gagtccgtat cttttccgat gccggttgtc ggtcaaagcc ttcggcaaaa
    25981 acaatattta attgctccga acagaaattt gcaatattcc tgatctgcga aacatccggt
    26041 ttgttatttt tcagtttccg tatgtgcgaa aatatttcct cctgcatccg gatcgccaga
    26101 ccgcccagca tttttctgtg gggaatgatt ttttcccaca acgtttccgg ggccgcatta
    26161 tcgatatttc ggacaaaaat gaaatccgca tccaggtgct tcaggttttt cagcaaagcc
    26221 ccgtgccctc cgggacgaaa cacaatacgc ccttggtcat ctctgaacgg aagattgttt
    26281 ttatcgacgg ctatcgtgtt cgttgacgcc gactgtacgg acagagagat attatatctg
    26341 atccggtttc ttttttcata tccggcgata acctttttca gataccgggc cactccggat
    26401 ttgtatccgg gggacagggt gaaatgaata tgacatactc ctcggttgag ggtgtacaac
    26461 gctgcttcgc acacgtgctc ctccagaggc gtgcgaattt cccgtgcaga ataacggtga
    26521 aagggaatca gcgctttggg acgcttcccg aaattcatcc ccccggaaga caaaataaat
    26581 ttcagcaaac ggccgacatc cttttcggca agcagtttcc gacccggatc gttttgccgg
    26641 atcagatcga aaaaaggaaa tttttttaaa tttgcaacaa ataatctgtc gagggctttc
    26701 gatccgaagc cccccttctc ggatgctgaa aaccattcgc tgaacatgcg gctggcggca
    26761 cccgatgccg gaacaaattt gatgatgtcg tatttacacg attcccgttc atagaaagag
    26821 attaatgccc ggcgccgtgc cgaagaaaag gaaacgatcc cgtcatgatc tgtgcacggt
    26881 ctgtttaatt ttaggaataa cggccccttc cggtgcaagg tcaactgcct ttcgacatcg
    26941 ttgagattca gtcgcaaacg ttccatgttt tgcagatctt ttccggaaaa cagcgaatgg
    27001 gcttcgggtt tgatcttttt tttcatgcct tttttcataa caccaacatg aaacgttgac
    27061 aagaggaaac aacttgcttt atgatcacct caatgaatat gtttcttttt cccgtttaac
    27121 cattgaataa atattttttg cctccgggtg ataccccgca tcaaacaagg agataaggcc
    27181 atgaataaga tttataaatt ttttacgctc atcatcatga ccatcgcctt ggtcattgcg
    27241 gcggaagaaa cggcaaacgc tcagatcggc tggcaaaaat ctgccggaca atctctgatt
    27301 gacgcactga aagtcaaaga gcccctgacc ttctgcaacg aaaaagtgcc tttaaaggaa
    27361 aacgacgtca gagaaagact ggaaagagag atgcttgtct ccctgcagaa cagcgatgat
    27421 gtcatcctgt ggctcaaacg cgcttatcga tattttccgc acattgaaat ggttttgaga
    27481 gaaaaccgcc ttcccgacga tctgaaatat atcgtgattg cggaaagcgc tctgaaaccg
    27541 ctggcggcat cccacaaagg agcggtcggt ttctggcaat ttattgaaag caccgggaaa
    27601 aggtacaata taaccgtcaa ttctgatatc gacgagcgcc gcaacttttt catcgccacg
    27661 gatgcggccc ttgcctattt gaaagatctt tattcgattt tcaattcctg gacactggcg
    27721 gctgccgcct acaacatggg tgaagacgga ctgaaaactg aaattttaat gcagcaaaca
    27781 aacaactatt atctgcttca tctgaatcag gaaacgcaaa gatacgtctt tagaattctg
    27841 gcggccaaga ttatcatgtc tgatcccgaa aaatacggat ttcatttaag taaagacgat
    27901 ctgtatcctc cgctgcaatt tgaccgggtg gaaatctcag ccgacaatcc cgtcccgata
    27961 caggtcatcg ccgctgcggc caatacgtat tttaaaataa tcaaggagct caatccgcat
    28021 ttgaagggtt atgatcttcc ccccggtcgt cacagcgttc ttattcccaa agggacagcc
    28081 gacggatttt ccgggcgcta cgagaacttg ctgaatcagt ggcatatcga aaaggaaaac
    28141 gccacatata cggttcaaaa aggcgacagc atttacacca tcgctaaaaa cctgaatgtg
    28201 tcggtcaaag ccattatgat ctggaacgac cttgccggcg tcaaccagaa attgtctccc
    28261 ggcgataaac tcgtcatctt tacccggaac cgttttttct gattgcacac catccgctgt
    28321 gatgacgctt ggtatcctgt gcgcttttgc atttttaagc agcacggcta tcgattcctg
    28381 aaaaagcggg ggctcttaat tcttgacaaa tcttttgttt tgatttaatc gccgtcaaag
    28441 cagagggacc ttatacagac gcgataaaca gcaggagcac tcatgaccat tgtcgacaga
    28501 atcaataaac tgaaacagga aaaaaatgca attattctgg cgcacaatta tgagctgcct
    28561 gaaattcagg acattgccga tttcgtggga gactccctcg ggctgagcat ggaagcggca
    28621 aaaaccgacg ccgacatcat tgtcttctgc ggtgtccgct ttatggcgga aaccgccaaa
    28681 atattatcgc cgcaaaaaac cgtattgctg ccggacaagg acgcagggtg tgcgatggcc
    28741 gacatgatcg aaccggaagg cctcagagcc cttcaatcca gacatccggg ggccgtcacc
    28801 atgtgttatg tgaattcctc cgcccgtgtt aaagcgttgt gcgattattg ctgcacgtcc
    28861 gccaacgcgt tgcccatgac gcggaaaatt gccccggatc accgggatat catcttcgtt
    28921 ccggatcaaa acctggcccg ttacgtttcc gcgaacgcac aatgtcattt tattatctgg
    28981 gaaggatact gtccggtaca taccgatatt ttaccggagc acatccaaca ggcaaaacgc
    29041 cttcatccgc aggccaaagt cattgtccat cccgagtgca agcccgaaat cacggcttta
    29101 gcggatatcg tcacctccac cgaaggtatg agccgccacg cccaaaactc tccggacaaa
    29161 gaatttatca tcgggacgga aacaggcatc cttcaccgat taaagaaaga aaatccggat
    29221 aaattttttt atcctgcatc ggacaaggcg atctgtgcgg atatgaagaa aatcactttg
    29281 gaaaaagttt tacactcttt ggaaaccctt tcgtatgaga ttacggttgc ccctgatatt
    29341 atggacaaag ccaggctctc catagaaaga atgctacaga caacgtaata ttgccggatt
    29401 gttgatgcca tgatgaaaaa gaaaattatt tcgtccgata caggtcccat ctctggtcct
    29461 tattccgcag cggtggaagc caatgacatt atttttattt ccgggcaact tcctgttgat
    29521 ccggcaaccg ggaaaatcat cacagacatc aaaatcgcca ccggacagat tctgacaaac
    29581 atgcaggccc ttttaaaaaa aaccggcctg gatttgtccc atatcgtaaa aacaacaatt
    29641 tttttgaaag acattaaaga tttcccggcg gtcaatgaag tctatgccgg attttttccc
    29701 aaggatcctc cggcccgttc aacagtggaa gtcagcgtat tgccgagaga cgtaccgata
    29761 gaaatcgaag ccatagcggt cagggaataa tgcattgccg ctgccggctc cttcgtggag
    29821 cggtccaaga cagattaatt cataaccgat ttctccacag gacgaccttg ataatttccc
    29881 aaacaggcat atggatggaa ccgcggttct atccatacat agaggtaaaa taacgatgcc
    29941 gacaaaacat ccgataaaaa gcaaaaacaa aaaaacatcc ccgtacccaa acgaattttc
    30001 tctcctgtcg gaagaaatca tcgcgaaggc cggtgtcgga atctacatcg tgcagaatcg
    30061 gaaattcgta tacaccagca atctttttca aataattacc ggttttacag atgacgatct
    30121 gatcggggca tcccccctcg atcgtgtgta tccggaggac caggaaaatg tccggcaaca
    30181 tgccgtcaaa tgtttaaaaa aagaaatttt cgagccctat gaattccggc tgttcaacaa
    30241 agacaatcaa ctcagatgga ttctggaaac cgttacgccc atcgtttata aagggcaacc
    30301 ggcgacactg ggaagtttca tggacattac cgaacggaaa atgacggaag aagccctgcg
    30361 ccaaagcgaa gaaaaatacc gaaccatcat cgaaaacatc gaagacggct atgtcgaaat
    30421 ggatctcaag gggaatttca tcttctttaa tgatgccatc agcaaaatgc acggtttcac
    30481 cagcatagaa ttactgaaaa tgaattatac agatgtcatg gatgaagaaa atgccgcgaa
    30541 aatctatgca aaatacaatc acgtttttaa gaccggcata ccggagaagg attttgaata
    30601 tcaaatcatc accaaagacg gtcaaagaaa atttctggaa acatccatat cgttaatcaa
    30661 caactctgcg ggaagaatca ttgccttccg cggtatcgtg cgggacagaa cgcagcgcaa
    30721 acaggcggaa gaagccctgc gccaaagcga ggagaagtac cgaagcattc tggaaaatat
    30781 cgaagaaggc ttcttcgagg tggatcttgc cggcaatttt actttcttca atcattcgct
    30841 ctgttcgctg ctcggttact ccagagaaga gaccttggga atgaattaca ggcaatacac
    30901 cgataaagac agtgcgcaag acgttttccg cacgtttaac ggaatcttcc gaaccgggat
    30961 gccggtcaaa gaattcgact ggttcattat ccggaagaat aaaaccagac gatatgtcga
    31021 ggcctctatc tatttaaaga aagattcctc gggaaagccg acagggtttc agggttttac
    31081 ccgcgacgtc accgatcgca ttcaggccga attccaaaag gaggcggcgc ttgaagctct
    31141 ccgtgaaagc gaagaaaaat acagaaacat tctggaaaat attcaggagg cgtattttga
    31201 agttgatctt gccggaaact ttacattctt caacgatgcc ttgtgccgga tgaccggttg
    31261 ctccaaggag gaactgaggg gaacaaatta tacaaaattt tcagatgaag aaaactcgaa
    31321 aaaagttttt caggtattca ataaggtttt caataccggc caaccgacgg aaggctttga
    31381 ctggctgatt atccggaaag acagcacaaa aagatacatt gaagcgtctg tttcgctaaa
    31441 aaaagattcc catggacatc ctgcaggttt taaaggagtc atccgggata tttctgaacg
    31501 caagcggatc gaacaggaat taaattacat ggcaacccat gatgcgttga ccggattgcc
    31561 gaaccgttta atgttcaacc agttgctgaa ccaggcgatt ctctccgcca agcgccataa
    31621 aaaacagctg gccgttttct ttattgatct cgaccgtttc aaaatcatca atgacacgct
    31681 cggtcacgat gccggcgacc ttcttttaca ggaaatcgcc agacgcttcc ggcattcgtt
    31741 aagagccgtc gatgtcgtcg gccgtcttgg cggtgatgaa ttcgtgattc tcatcgaaga
    31801 tttcagcgac ttcgcccagg tgtcgaaagt ggcgcacaaa attctttccg ccgccatcaa
    31861 gcccatggtg atcatgtcgg aagaatgccg cgtcaccgcc agcattggaa tcagcatctt
    31921 tcccaaggac ggacaagacg agcagtccct gatgaaaaac gccgacatcg ccatgtattt
    31981 tgcgaaagag gaaggtaaaa acaattttca atactattcc aaagacatca aaacgcaatc
    32041 gaatgaacgt ctctcgattg aaaccaatct tcgacgggcg ctggagcgcg acgaatttta
    32101 tctgacgtat caggcgagat tggatttcaa atcaaaccaa atcaccggtg tggaagcgct
    32161 gcttcgctgg aacaatcctt atctgggcca ggtgacacct acgcaattca ttcccctcgc
    32221 cgaagaaacc ggtttgatcg ttcccatcgg cagatgggtg cttaaaacag cctgcgcaca
    32281 aaacatggcg tggcagaagc agaatctgcc gcccatctgc atggccgtca acttatcgct
    32341 gcgtcaactc tcggacgaaa acctgctaaa cgatatccgg atggcattaa acgaatccgg
    32401 catgccgcct caacttctgg agttggaaat cacggaaagt atggtcatgc ataatcccgc
    32461 aaagctcatt caactgctga ccaaaatcaa ggacatgggc gtccggctgg caatcgacga
    32521 tttcggcacg ggctattcct ctctggcaca aatcaaacat tacccgatcg atacactgaa
    32581 agtggacagg tctttcatcc ggaatcttcc gaaggattcc gaggataagg ctatcaccga
    32641 agcgatcatc acaatgggga aaaccctcag cctgaccgtc gttgccgaag gagtggaaac
    32701 cctcgagcag gaaaattatt tgcgggagca cgtttgcgat gaaatgcagg gcttttattt
    32761 cagcaaaccg gtcaaagccg atgattttgc tgaactgctg cgcaatcata ccggtgcaca
    32821 tctgacatga aattatgcaa aaagaagtgc gccctgttcg caacgtcctt cactgacggg
    32881 cggctttttg atccaggatg tccatagcgt ctttcgccgt gtttgctacc gcgtcattat
    32941 tgttgcggga aagcgttttt aaaaactcct tcgattttat ccctccgcat tttcccagcg
    33001 atacggcaca ggcgcacagg atatcctgtt tttcccgggg aatctccgct ttcatgaatt
    33061 ttagaatccc ttttttttcg ttcagcaatt cggcggccat ctctatgatc tcattctcca
    33121 gtgtttcgcg atcagaaacc tctccaagat gaccggccgc ttttaataaa ccgcacacca
    33181 acccggcatc atttgcattg atattcttct ttgagattct ctcttttaag aacgatacta
    33241 ttttttggag catctctaca gaaacaggca gttcatgctc gatcagactc aaagcctgat
    33301 gtctgacctt ttcctcttca tcgttcaaag cgtctataat gacggcggcg gtgtcctgtt
    33361 gattgatttt tgccatggcc gccagggctc tcgctctgat gctggcattg gtgtgcttaa
    33421 gaaatttcct taccgttttg ctatcctctt tttgtccgac ctgcatgatg acgagcaaag
    33481 cggcattcaa cacggataac ggatgattcg actgtccgag aacatccatc gcccatcgcc
    33541 tccctgtttc tcccttttgg gataatatct ccaccgcaca tttcaaaatg ttcggctctt
    33601 gcgacttgct gatcatcagg tcgacggttt tgatgcacat cgtgttcgac agcagcaaaa
    33661 cctcgtcgat tttgcttcgt aaatttgcat ccccctggat gtatttatcg gctatccttt
    33721 cttccatgcc ttcccagatg gaatcaggca tacctttaag aaattccgat tcctgaatca
    33781 tgtcttcatg atgagcggac agatcgttta gagcctgcac aatccgtgcg atgattttcc
    33841 actcaccggc attgacgaac gcggggatga ccctcttgac cgttaagaca atactttccg
    33901 cctcctcaat ggaggacgcc ttcgagatgt tttccaggta attctctgtt tgcgataata
    33961 tttcatcggc cagttgagcg ctttccctgt caatacgcat gtccgccgga agcatatctg
    34021 aatcgataat tttacattcg aacaattgtt caaataaatt ttttatatcg ggcattttct
    34081 ccgcaatcat tctttctgcc gcgatgttga gaactttagc cagataaaca tttctttcga
    34141 gccgctgttc attctcttcc tgcgaaaccc cgtccatcag gttatcccgg taaatatcaa
    34201 atactgtcgt ggaaatcggc acgacggcat cagattgcaa tgaactgatt atcagttttt
    34261 ccaactcatc aacctccatg aagtgggaga catgatccac gacaatatcg cagttgacaa
    34321 tcaggtcttt tagtaaatca ttattcgtga ggggacggat gatatcttcc acaatctgct
    34381 ttttgattac cttcatttta tcttcggtgg caaaacggaa catcggcacc acgttcagat
    34441 ccttggcgag acggcgcaag atgatggata cacgccaggg aagtttccct ctggaaagga
    34501 caatatctgt tttgaaaata gtcgatactt cagtgatccc cctgtcaaca agagattttg
    34561 tgaggtattc tcccagtttg gatgtgtccg cggattctgc aatcggctcg ctcatcacat
    34621 cgataaaaga ctcgaagtgc tcggctgtaa tatttttttt gatgacaaaa ctgttgagac
    34681 tttttctttc aaagtagtct ttcagtttcg gcacaaacag atccgatgtg ctggattgtg
    34741 ttaattttct tatattgaac ggttcattga gaatgccgct gatatggatg tccaccttat
    34801 cctcgtactc atggcaggaa agcatgattt cggaagaatc gcccagtgtc tttttaaaag
    34861 cgatgtagag gccttttttg acttcctggg aaaccgggtg gtggctgtca taatagccgc
    34921 tgcgggaaat ggccttgatg agttcaataa cgaattcgca ggtcgttttt gccttggcga
    34981 actcatccgg agtaaattcc tttttttcat ctttcttata actagttttc tcaactgttt
    35041 gcatcgtatc agccctttcg ttttgatatg gtgtaacaac agatcgatta aaactatatt
    35101 cataagaaag cggatcaatg atgccggata cgcttttttt tcgacgcatt gccgtttgaa
    35161 tgtatgagat gtttatatat aacatgtctt gaaagaaata caaatcacaa atatcatccc
    35221 ccgtctgatt tctgtaacac cccgggttga caaaagaccg aaagacgttg taagaaaata
    35281 aaacgttgag gctccgagga tcacaataaa tgatgaatca cagacatcca ttacacggcc
    35341 gttccacggg aatgtttcgc aggattattc tttgcttggc gctcatcctt gccgtagcgt
    35401 cactggtcgc ctgttctctg atcaataccc tcttccctcc ccccgccaaa cccgccaaaa
    35461 ttgccctggt gctgggcggc ggcggttcca aaggattcgc gcatatcggt gtgctgaagg
    35521 ttcttgaagc gcagaaaatc cccgtgcaca tggtcgtggg cacaagcgcc ggcagtgttg
    35581 tcggcagtct ctatgcgtct ggcaaatccg ctttcgaatt gcaggaaatc gccctgaaaa
    35641 tggataagga taagattatt gactacgatt ggaaaatatg gagcggcggt ctgatcatcg
    35701 gcgacaaact ggaaaatttt atcaatacgt atgtcaaaaa cacaccgatt gaaaaactta
    35761 agattccttt ttatgccgtg gccaccaaca ttgccaccgg tgaagaaacg gtttttgccc
    35821 gcggcaatac gggaatggcc gttcgcgcca gctgctccgt tcccgctgtt ttccagccgc
    35881 tgaaaatcgg gaataacacc tacgtggacg gcggtgtggt cagcccggtg gcggtcaatg
    35941 ttgcccgtcg taacggtgcg gatatcgtta ttgccgtgga tatttcagga ggactccaaa
    36001 gaaccgttcc ggacggcatg ttcgattcga tcaaaaaatc tgtggatatc atgtattcca
    36061 aaatcgccga atatcagatc aaacatgccg acgtcgtcat ccgtccgaac atgaaacata
    36121 tcagctccac cgaaatggaa aaattcaatg aagcgattct cgaaggtgaa aaagcggcca
    36181 cactgaagct tcctgaattg caacagataa tggcacaatt aaaacaggaa agacgtctct
    36241 aaagctttct ttttagcatt ctgcaatcga gtatttatga tgattatcgg cgcccgggcc
    36301 cacaatcttg atgaagccaa caccctcagc aaaatgcatt gcccctatat tgaaatatcg
    36361 ctggatgatc ccgctgtggt ttcgggctgg cttcctcaac tgcggaacat cctgaaccgg
    36421 gacggcataa cgtttttggc gcattacccc aatgaagaca acccctttga tgtggcaatt
    36481 cttcgcaata cgtttctccc ccgtatcgtc accttgatgg aattatctca aaaacttgac
    36541 attcataaag cgaccattca cttctggatt gacaaacgcc gtctgcctca tgatctgatc
    36601 cgcgacaaac tggacattct ttcccgtatc gtggactacg gcaatgattt cggcattacc
    36661 gtctgcatag aaaatttgag cgaacgcgcc gacagtttcg cccccgcctt tgacgccatt
    36721 cccgatctga aaatgacgct cgatatcgga catggccagc tgctttcacg gcaaaacaca
    36781 tcttttcgct ttattgaaga acattttcct cgcatcgcgc acgtccacgc ccatgataac
    36841 catggaggaa aaacagtaag tgacgacctt cacctcgccc tgggcgacgg ctccattgat
    36901 tacaaaagga ttttcaaacg actgattgcc aagggatacg cctcaaccat cacgacggaa
    36961 gtcaaaccac aggatctgct caaaagcaaa cagcggctgg aggattgcat ccgcagcatc
    37021 actcctgcac aaacgggtaa cggcttgtga catccgcaaa gatctgtccg ttcttttctt
    37081 tcaggataac gaagcccgca tgaacaaccg aactcacgtc taaaatcgtc ccgatatttg
    37141 aactcaggcc aagggattcc aaatcgctca aatgagtcag atttgtcgag ttgatgtccg
    37201 tctcactatt ataagccttg tgatgttgcc tgagcacctc agcgttgtat tctattccgt
    37261 attcggtcag ataggacaag gctttggcaa atttttcatt tctgtaaccg gcaatggttt
    37321 ctttcaggcg attggcgatt tgggcaatgg acatggcatg aatttccttt ctcgtaaatt
    37381 ccgtcatgct ggtaaacgtc gcattgccga gatgcagagg gtcgatatct ggatgcagat
    37441 cccgcagatt gatcccgttt cttaatctca ccttgtcggt atcgcgcagg atgtgatcgt
    37501 gatatttttt caataaaaat gaaataataa tttgattact ggaaatggaa caggtctcgt
    37561 tttcctgttg ggctgtgcgt ttcatatccg caagaaattc atctgaaaag aattcccttc
    37621 tggtatataa tttttcatct ttttcataat gtccggcctt atcttgaatt tcacggctga
    37681 gttcggaaag cgacgggaga aatactctgt ggattttatc agaatcaaca ggcagccctt
    37741 tgaataatct ttgaggagat ggaggcagaa aaggcctgcc ctcagtgata cacatccagg
    37801 catagaggaa aagaagcatg gaaatgccgt ctgccaccgt atgcgaacag cttatgccgg
    37861 ccaaaatccc gtctttgacc ggaatgcccg taatagcaaa aagcggttcg cccggcaatg
    37921 ttttaacgtg gaccatcatc tttttaatgt cttcaagact gagatcacgg gaaacagcgt
    37981 caagcggcgg caactcattc aacacggcgc cgtccgtaca atattgcaat gcaaacttgt
    38041 tttcaccgat cgcgatcaga cgactggaaa agagattata atgttcaatg gttttgatga
    38101 tgctttcttt gagtttcccg atgggaatgt ctttcctgta aaaatataaa atttcattcg
    38161 gacttccggc gatcatccgt tcattttcaa gaacggacaa tggcagaata tttttgccgt
    38221 aattttcttt aagatgggca gtatgttttt cattcatttt catcgtttct ccgtaaataa
    38281 aattttaaat ccgagcaaca accgttacat ccggaacatt gaaagttcaa tggctctact
    38341 aattaatatt ccttatttat caattactta aatctatttt atgattcttc gctttataat
    38401 ccggtaggcg atcaggacaa tggccagggc cgtaaccgaa agagacaaaa tccatttcgt
    38461 attggtggcc agccccgata ttgcaaccag aacgggcgtt gcccggtcaa ggtccgcaag
    38521 gaaatagatg tgcatcgtgt tttcaaatag atcgcacagc cccgcaatga acggtgtcag
    38581 aacaaccccg ttgaatttcg gattgacatg gttcatctta aaggcccggg cgaccagcaa
    38641 actcaggaaa atactgtacc agagggggtg aaagtaatcc agatagaagt gctgataata
    38701 aattccgatt tgtccggacg caatccatcc ggacgcaatg tccttgaatt tatcgctgct
    38761 gagagttgtc tggagcataa aggcctgaag cgtgcctatt ttatgaaaaa tcgtaccgag
    38821 aatgatctgc gatgtaaaat aaatgacggc aaacaacgcg atgagaaaat tggaatccga
    38881 ttttttcagc acataatttt cagcccctgt caatcttccc ataatcggcc tccggagttt
    38941 ttcgatatgc ttttgaaaac ggaaaaaata taatcttatt gacgtgatct gtcaatcaca
    39001 agctgcctgg atgaaaccgt ctatggcgtc ttttcggtgc atgcccgata aaactgcgct
    39061 gctcggttat attcatcaat catggaggac agcaccgccg ttttgccttt ggcttccccg
    39121 acgattttgt tgtaccagcg gctcttttca ttatatgcgt aaattttttc attgacgttc
    39181 tgaatttttt gaggatcatc gtacgtgtat tttttatccg tatcgatttc tgcctgttgc
    39241 gcgtccagct cgtttttcag acggtcgagc tggttttgca aagctgcgat ctgcgccttt
    39301 ttgacttcaa tatcacgcag gagatcttcc gaaggcgcaa cacaaatatt gtgcggcgtc
    39361 ggcaacgact gcaaaccgaa tatctggacg gcaacgtaag tcggccttcc tttcattttt
    39421 ccttccagca cagccgctcc catttcccgg atgtcctcgg aaagcatatt gtcccgatgt
    39481 ccggggcttt gcatccaccc ctcgacgatc ttctgattgg agtgaaaaac gcccttcccg
    39541 atattttccg cgataatctt ataggcataa ccgacatctt cagccaggtc cgatgcctgc
    39601 tcgcccgtgg gagaaacatg cgcaaaatac tgttttccca gcatatccct cgctctggct
    39661 tctgcgattt gattcaacaa gcgattctcc gcaagttcag caaggccgtt atcggttcgc
    39721 gccttgtttg tcagacgaat aatggattcc ggattcagtt tctctccgta aacaacgggc
    39781 agcggaatat gatcctgaac cctgctttgc aaagcagcat acgattcccc gatgttgtca
    39841 gcatgaaaat aatcccgaag cgtcggcata tctgtaacgg ccagcgcaaa aacaaaaccg
    39901 gccagcccca tgatcacgat gaaatgtttt ttcaaaaaat cgaatcctgt cggtttctgc
    39961 aatgctgcat catcctttat ttttatgtga aattatatga agaaagaaag ggaaggtcaa
    40021 ccgcagcttg ccccgttcag cgccgttgaa aaaccattag tattcaatat cagccgaagc
    40081 gggaatttcc tgcacgaact cgacctcgcg cgggactttg taattggaaa gataggtttt
    40141 gcaataacga ataatttctt tgtcgtccgc gaccgctccc tctttcagaa taacgtatgc
    40201 cttgacgatt tcaccgcgca tcagatcttc cttgcccgaa actctggcgg ctttcaccgc
    40261 cggatgccca ttcaacacag tttccacttc aagcggataa acattaaatc cgctcgtgat
    40321 gatcattctt ttcttgagcc cggtgataaa aatataaccg tcttcatcca tccggcccag
    40381 atcgcccgtg tgcagccagc cgtttttgat gaccgccgct gtggccgcct cattcttgta
    40441 atagccctgc atgacattct ctcccctgac aatcaattcg ccgatctgat tgcggggcag
    40501 ttcgcgtccc tgatcatccc cgattttaat ctcaacgcct gttatcgccg tcccgatgga
    40561 tcccggcttg taaacctgat cgaatcgatt gaaagaacat acgggcgacg cttccgtcag
    40621 tccataacct tccagaagtt tcagattgaa tttttcttca aagacatgca tgaaatcggc
    40681 cggcatcgcc gatccgccgg tgacgcaaag cttcagcgaa ctggtgtcgt accgggaggc
    40741 tttttcgtaa aaaatcattc ccataaacag gcgcggtacg gctgaaatat acgtaatttt
    40801 ttccctttct atggaaccga aaagaccatc catcgtcagc cggtccatca tgacaatgga
    40861 acagcccgcc cggatggtaa gaagcatatt ggccgtggct ccgaaagcat gaaaaagcgg
    40921 aatcagcgcc agcgcaacat catccggcgt tctctgggca atggattgcg ttacaaccgc
    40981 ctgtgaatat aaattgtgat gcgtcagcac cgcgcccaga ggtctccccg tcaacccggc
    41041 agtatagatc ataaccgccg gatcttccgg cttgatctcc accggtgtga aggacgctcc
    41101 ctgcgactcc gcgcccggaa aaggctggcc ttccggactt aaggcatcga cggcaatgac
    41161 ctgccggcat gtggaaagat tatccttgat ttcgtgatat tttttgagct gagaggattg
    41221 tgtgattaaa attttcgaat ccgaattatt caggagataa atcaattcag acggggttgt
    41281 cagcggattg atgggaacaa ccaccgcccc gtttttgaca atgccgaaat aacagaaaac
    41341 aaactccggg atattgggaa gcataacggt aactttatcg cccttgccta tgcctcgctg
    41401 ctgcatgaat ccggcaactt cgcccacggc ccgatggagc tgttcgtaag taatatgctg
    41461 cttgtcgctg atcaaagcga tgtgttcagc gtttttttgc accgcatctt cgagtaattg
    41521 aatgagatta atagccctga aacctccccc caaaattgcc ggtcacttaa cataaccctg
    41581 aatcattttt caagaaaata aaaaccggtc gctcgcgttt catccggcgt tttttgtata
    41641 acaattaccc gcaatagtct aatcatttat tcatgtttct tcgggtcggc atgaatatcg
    41701 gcatcgggtc atcggataaa attcgtccat atgtgttctt ccggctccgg ccggacaatt
    41761 cccgctttcc gtcccgcggc gatggatttt aaaagccagg ccatgttttg ccccagcgtc
    41821 cgcatgatct gcaaaccttc gagatcctgc aagacttctt ccggcgtgtt gccgtgaacc
    41881 atgttccagt actgcgacga gaccaccggc atgttgctga tggtaaaata tttattgagc
    41941 tgatcaaagg cggcggttgc ccctccccgg cggcaactga caacggccgc gcccggttta
    42001 taggcaaaga catcgctgcc tgcaaaaaaa caccggtcca gaaatgatgt aataaatccg
    42061 gaagcccccg cgtaatgcac gggagagccg aagacaaaac cgtccgcctc ggccgcttta
    42121 ccgattgcaa cgttgacggc atcatcatcg aagatgcact tccccgtctc cgaacatttc
    42181 ccgcaggccg tacaccctct gatcggttta ttgccgacgt agaatatttc cgcgtcgata
    42241 tcctgttttt ttaatgcctt tgccacctcg gaaagagcgg cataagtgca tcccttcttg
    42301 ttgggactgc cgttgatcaa taatacgttc attttaccta cctcttcttt taatgaacat
    42361 catccgatac gctttgtgtc tgaatacgat tatttttctt tttttcatac taagccaatc
    42421 tttcaaaaaa tacaatgagc cattcgaaaa gattgttttt ttcttcccct gtccggtttc
    42481 caatgcacaa aatgacaatt cttcctcatg attaaacgtg aaaacctata taatttcatc
    42541 ctatttcctt tctttttttt aagcctccgg gcggttgtca tttgtccccg cccatctaca
    42601 aatccccgga tttaacccgt tgacaaaatg accggcagtt tctatacttt cgattatgtt
    42661 tgttcggata taaacataaa gttcaatcaa gaaggagaaa caaacatggc atcaaatttt
    42721 ttgtattcca ctcgtgaaca gaagttcatc ctgaaagaat ggctagatct gtccaaggtc
    42781 tttggagcgg gacgtttcca gggcggctac agcgttgatg atatagattc catcctcgaa
    42841 aacgcgatga aaatagccaa actgtcgata gcccccacca gcgacgacgg cgacaacctc
    42901 catgccgtct acaaggacgg taaagttacg acacccgaat cctataaaaa aggctattgg
    42961 gccattcagg aaaacggcct cggcagcagc aacgccgacc acaccgaccc cagttccatg
    43021 ccgctgtgcg tgatgggggg caccaatgag tatctggcgg cagccaacgc ctcattggga
    43081 ccctatgcca tggcgaccac cggttccgcc ggcctgatcc gcgacttcgg
//
LOCUS       DAOSME010000015        40566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1296_length_40566_cov_19.068722, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000015 DAOSME010000000
VERSION     DAOSME010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 40566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40566
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1296_length_40566_cov_19.068722"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..2061
                     /gene="thrS"
                     /locus_tag="PLG98_03700"
     CDS             148..2061
                     /gene="thrS"
                     /locus_tag="PLG98_03700"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOE32386.1"
                     /translation="MTIKITFPDHQELSFNRSTPAAEAVSAWKKEALAHTVAVKINGI
                     PADLSRPLDRDATIDLIDLSSAEGLSILRHSISHVMAQAVQDVFPGAKVSIGPSIEDG
                     FYYDFEYHETFTTDDFPKIEKRMKEITKADNPFVREELSREAAVALFEEREENYKVEL
                     INDLSHDVKTVSLYKQGGYVDLCRGPHIPSTGMIKAFKLLSVAGAYWRGNENNKMLQR
                     IYGTGFATENELQGYLTLLEEARKRDHRRLGRELDLFQVNDEAGAGLIIFHPKGMLLR
                     YLIEEWERKEHLKRGYDMVMGPQILKVDLWKKSGHFDHYRENMYFTEVDEQVYGIKPM
                     NCLSHMLIYKSKIRSYRDLPLRYFELGTVHRHEKTGVLHGLLRVRQFTQDDAHILCTP
                     EQLNSEIRGIADFVSHAMGIFGFEYEVELSTRPEKSIGTDADWDLATSALENALKDNH
                     IAYDVNAGDGAFYGPKIDFKLKDALKRKWQCATIQCDFTLPERFDLSYIGPDGEKHRP
                     VMLHRVILGAIERFMGVLIEHYAGAFPVWLSPTQCVLLTVTDKHIPYAQTVHDKLIAS
                     GIRSERYFDNEKLGYKIRQAQLQKIPYMLVIGDKEMDAGAVAPRTRDGKNMGAMPVDQ
                     FITLVHQTCDQNK"
     gene            2125..2619
                     /gene="infC"
                     /locus_tag="PLG98_03705"
     CDS             2125..2619
                     /gene="infC"
                     /locus_tag="PLG98_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOE32387.1"
                     /translation="MNREIKSANVRVISEDGKQLGVISLEEALAKAEEAGLDLVEVSA
                     SADTPVCKIMDYGKYRYKQNKKLHDAKKSQTVIQVKEIKLKPKTEEHDVQVKMKHIKK
                     FLKNHDKVKVSMMFRGREIAFTDIGRKLMGKIRDELANECIIDQEPRLEGRNMVMILS
                     PKKQ"
     gene            2638..2835
                     /gene="rpmI"
                     /locus_tag="PLG98_03710"
     CDS             2638..2835
                     /gene="rpmI"
                     /locus_tag="PLG98_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOE32388.1"
                     /translation="MPKLKTHRGAAKRFSLTAKGKIKRSKAFASHILTKKTTKRKRTL
                     RKSTIVDSANAREIKKLIPYM"
     gene            2884..3234
                     /gene="rplT"
                     /locus_tag="PLG98_03715"
     CDS             2884..3234
                     /gene="rplT"
                     /locus_tag="PLG98_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOE32389.1"
                     /translation="MSRIKRSVNARKRRKKVLKLAKGFFGARSRLLRTATEAVNKAMK
                     YAYRDRRARKREFRQLWIARINAAASDNNITYSRLIDGMKKSGIELDRKILAELAVND
                     PQGFAQVVTIAKGK"
     gene            3244..4260
                     /gene="pheS"
                     /locus_tag="PLG98_03720"
     CDS             3244..4260
                     /gene="pheS"
                     /locus_tag="PLG98_03720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOE32390.1"
                     /translation="MMKELQQLESDALSELSTATSEEQVLTVKTKYLGRKGLLTGLLR
                     NISRVPDEEKPLFGKRCNELKNSLNARLDQAIEQYTSRNKEQALFKEKIDVTLPGRSV
                     NCGRVHPVVQIRREICSVFSSFGFSIVEGPEIELDYYNFEALNIPKDHPARDMQDTFY
                     IDENIVLRTHTSPVQIRVMEKIKPPLRILSPGKVYRRDSDVSHTPMFHQIEGLLVDKN
                     VTFGDLKGILSAFLKKIFGSSTMLRFRPSFFPFTEPSAEVDICCVMCGGKGCRVCGQS
                     GWLEILGSGMVDPAVFRNVDYDPDEYSGFAFGLGLERIAMLKYGISDIRLFFENDIRF
                     LKQF"
     gene            4275..6686
                     /gene="pheT"
                     /locus_tag="PLG98_03725"
     CDS             4275..6686
                     /gene="pheT"
                     /locus_tag="PLG98_03725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOE32391.1"
                     /translation="MLVGLTWLKDYVDVQLTAKDLADKLTMAGLEVDEIKKVGPSFSG
                     VVVAKIISVGPHPSAERLSLCDVHDGKDLYRIVCGAKNIAPGNIVPLAKVGAVIPGGY
                     TIKSSVLRGEKSDGMLCSEAELEIGSDATGIMQLSPDLVLGQPLEEALNLEDTVLDVN
                     VTPNRPDCLSMIGIAREVAALTGRKIKWPAVRIHEKAPDIHSLTSVKIIDSDLCPRYT
                     ARVIKNIAIAPSPRWMRLRLESAGLRSINNIVDITNFVMLETGQPLHAFDFRFLEEGR
                     IVVRRSLENEEFISLDGKIRTLPADTVLICDGIKPVAIGGIMGGLNSEVKEDTQTVLL
                     ESAYFRPTSIRSSSRKLGMSTDAAFRFERGIDPDGVVRALDRAAQLMVDLSGGSICKN
                     YIDEYPRKISSAGDIPLRLDRIRKIIGTKIAARDVLKILRRLDMTVKPEGKGRYLVTP
                     PTCRVDLEREIDLIEEIVRLYGYDRVPVTLPDVSVNELDVIARLDMEERLRQLLTGSG
                     FTEIVNYSFGTPSAADMLSLPATDERRNTVRIKNPLGEELSTMRTNLIYGLLDTAKRN
                     FNNGAFDLKLFEIGRIFLHRREGELPEEKTMIAGLITGKIRDDFWNSEQTVDFYTLKG
                     ALENIFCDFKIQNCRYAATTDESFLHPGKACHIVLYETVIGAVGEIHPDVLEKLDIRN
                     TIYVFEMNIDILSRVCEQNSLSFNEVSKFPAVIRDAAFVIPDPMEAEQMIDIVLSQME
                     ELLENVSIFDVYKGKGIPEGTKSLGLRFSYRANDRTLTDAEASAVHDRIVQKTVNVTG
                     AKLRS"
     gene            6721..6993
                     /locus_tag="PLG98_03730"
     CDS             6721..6993
                     /locus_tag="PLG98_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOE32392.1"
                     /translation="MTKIDIIQNVHEKLGFSKKDSSDIVEAVFDLIKDCLANGEKVKI
                     SGFGNFMVKEKRARRGRNPQTGQEISITARRVLTFKSSQVLRKALN"
     gene            7089..7433
                     /locus_tag="PLG98_03735"
     CDS             7089..7433
                     /locus_tag="PLG98_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOE32393.1"
                     /translation="METIIPDKAYFRIGEVSKILNVEPYVIRYWETEFKTVNPMRTKT
                     AQRLYRKKDVQELLTIKNLLYAQRFTIDGAKKQLQKLRNTDEPEEKNSEKQKLFLIRI
                     KQELQHIREIVS"
     gene            complement(7482..7763)
                     /locus_tag="PLG98_03740"
     CDS             complement(7482..7763)
                     /locus_tag="PLG98_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HOE32394.1"
                     /translation="MICKTVKAGFDCVFMTKNGCSYTNGSCQVVVEQCEGCGKIFEYE
                     SARYCKVYPDPAGKWLIGRCPTATHVKIEIKEAQKINPLKASKRASKKA"
     gene            7997..8446
                     /gene="dtd"
                     /locus_tag="PLG98_03745"
     CDS             7997..8446
                     /gene="dtd"
                     /locus_tag="PLG98_03745"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOE32395.1"
                     /translation="MRAVIQRVDRACVSINRQVFSSINEGILILLGVEKSDTEADADY
                     ILEKAVNLRIFEDDQDKMNLSLLDTQGDMMVVSQFTLLGNCVKGRRPSFDAAEEPVQA
                     CRLYDYFVRQAKTFIPGLATGKFQEMMKIDLVNDGPVTIILDSRKMF"
     gene            8449..8937
                     /locus_tag="PLG98_03750"
     CDS             8449..8937
                     /locus_tag="PLG98_03750"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HOE32396.1"
                     /translation="MDNFEIRIDKDGLWYYKGAHMFRKDILSIFYEHLRIDDSGRYLI
                     KLGPECCYLAVEDTVFVVLAVFKTKNADNRRDQIEILLNDDTCEILDMNSLRSGKENV
                     LYCRVKIGKFTARFSRKAYYQLAEFIESSEDGSRFFIPLNGQKYFIRQVQDSDACPTR
                     PS"
     gene            9081..10541
                     /gene="guaB"
                     /locus_tag="PLG98_03755"
     CDS             9081..10541
                     /gene="guaB"
                     /locus_tag="PLG98_03755"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOE32397.1"
                     /translation="MLDDTIKESLTFDDVLLVPAASNILPKDVDTTTSLTKNISLKIP
                     IVSAAMDTVTESRTAICMAREGGIGIIHRNMSIEQQAIEVDKVKKSESGMIVDPITID
                     PDRKVSDALKLMNQYSISGVPVVKKGKLVGILTNRDLRFEENLDQPVSNVMTKKNLVT
                     VSSNISLEKSKKLLHEHRIEKLLVVDDEYRLKGLITIKDIEKIRRYPNACKDSLGRLR
                     VGAAVGIIDREARVEALLAAGVDVIAIDTSHGHSAGVIKAVKATKSDFPKCELIAGNV
                     ATADGAKALMDAGVDAVKVGVGPGSICTTRIIAGVGVAQMSAIRDAYKIASKKGVPVI
                     ADGGIKYSGDIVKAIGAGAHCVMIGGLFAGTEESPGETILFQGRSYKVYRGMGSLEAM
                     KMGSRDRYYLDDIEAPLKTVPEGIEGRVPFRGSLSASILQLIGGLKAGMGYVGCRTIP
                     DMIKKARFVKITQAGLRESHVHDVIITKEAPNYWLD"
     gene            10596..14078
                     /gene="dnaE"
                     /locus_tag="PLG98_03760"
     CDS             10596..14078
                     /gene="dnaE"
                     /locus_tag="PLG98_03760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOE32398.1"
                     /translation="MKHSQFVHLHLHTQYSLLDGMIRLDELFKKVKAYAMPAVAITDH
                     GNMFGAIDFYQQAFKNGIKPIIGTELYVAPKDRKDKTPSSIGESTRHLVVLVKNKQGY
                     KNLIKLNSLAHIEGFYYRPRVDKALLRECADGLIASSACLHGEIASWLVRGNMEEARR
                     AAREYLEIFGEENFYLEIMENGIPEQKIANQGLIELSKELSIPLIATNDCHYLERDHA
                     EAHNVLLCVQTGKTIEDKDRMTMATDQFYVRSPEEMCELFESVPEALENTVKIAERCN
                     MTFEFGKFYLPKFEVKHPEETLEAYLERKAVEGLEKRMPSILKYSTGEENSIRKKYSK
                     RLHEELEIIKKMGFAGYFLIVSDFIKHAKHNDIPVGPGRGSAAGSLVAFAIRITDIDP
                     LRYGLFFERFLNPDRISMPDIDIDFCQERRGEVIKYVTEKYGKDKVTQICTFGKMLAK
                     GVIRDVGRALNIPYGDADRIAKLVPSIPPNITLNEAFKMEPRFEEERKKNPQIDKLLK
                     LSLILEGLNRHSSVHAAGVVISDAPLMERVPLFSPKDDIVSQYTMKDIETVGLTKFDF
                     LGLKTLTVIKNTLQFIRDYKDTEVDINNLPLDDPDTYKLLMKGETDGVFQLESEGMKD
                     LLINFKPDRIEDVIALIAAYRPGPMKMIPDFIARKHGKQQITYELPQLEPILKETYGI
                     ILYQEQVMQIANAIGGYTMSQADTLRKVMGKKKVADMEKEKPRFMEGAKKQKIDDNKA
                     RIIWDQMEKFAEYGFNKSHSAAYAMITYQTAYLKAHYPVEFMAALLTSEKDNRDKIIK
                     HMTSCKDMGINILPPDINESEKGFSISGENIRFGLTAVKNVGEAAIESVIDVRETAKF
                     LSFIDFLNRVDLRKVNKRVIESLIKCGAFDSMGHKRSQLMHSYEEAMEVAQRNQKEKS
                     SNQSSFFDQLESGSSSFENGLSAYELPDMPEWDHKKLLSLEREALGFYITGHPLLPYT
                     DRLKLVTDSDSENLLIRRDKETVTVAGVVSAINERKTKRNDTMSYVTLEDLHGSINAI
                     FFGDLYKTSYNILHEEEPVIVKGTLDISGTEENQKISLIAQEVTPLAEALKNPYKQVR
                     FMVDADRMSAEDISSFISSIRKFQGKYESYMHIMNGKSETIVYLGDDLRLDINERLKK
                     EADNILGQGATIFS"
     gene            14094..14816
                     /locus_tag="PLG98_03765"
     CDS             14094..14816
                     /locus_tag="PLG98_03765"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOE32399.1"
                     /translation="MPPYEERTYRSLIKNDHLISYNVKISESDLFIRSDTDLSDQAFQ
                     SLANHRHSLQIYIQNHSDFRTSLLPVDEDHLAPAIVRDMIDKSKICGVGPMAGVAGAI
                     SEFVGRDLLSSTKNIIIENGGDIFLQSQDTLTVSIFAGESPLSHKVHLEIKPEETPVG
                     ICTSSASVGPSLSFGSADAVCVLSPSATLADSAASAIGNRVKNNSDIKSALDFGMNIP
                     GIRGIVIICKKEMGIIGSVRLL"
     gene            complement(15079..16035)
                     /locus_tag="PLG98_03770"
     CDS             complement(15079..16035)
                     /locus_tag="PLG98_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOE32400.1"
                     /translation="MAEDYYQVLGVEKKASADDIKKAYRKLALKWHPDKNPNNKTAEE
                     KFKKISEAYAVLSDPEKRNQYDQFGSADQFRQRYSQEDIFRNFDLDEILRSFGFGGIK
                     GGRATFRTNRKRGGYEGEYDDPFAGLFGGAMGGNRARYANMPQKGQDAEYNLSITLEE
                     SVLGAEKKISFQLENRIEDINVKIPAGISAGKKLRLPGKGLSGFNGGPSGDLYLNINV
                     LPHPIFARDGNDLHIEKTIKFTQAALGTTIDVPTLDGTTKRIKIAPGTQNNTKIRMKG
                     FGVPGLKGALKGDQFVKINVDVPRRLTDRQIKIIQQLADDGL"
     gene            complement(16089..16670)
                     /locus_tag="PLG98_03775"
     CDS             complement(16089..16670)
                     /locus_tag="PLG98_03775"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOE32401.1"
                     /translation="MSENEVKNILFAGVGGQGILRASDIICEVIMEAGFDVKKSEVHG
                     MAQRGGCVTSHVRYGKKVYSPLAEPGSIQTLVSFEKMESLRYLSFLRKDAAIVVNTEE
                     VYPPAVNMGESPYPDDAVEFLQNNYKTVISFDASKLAQKAGNIKTANVILLGALSNHM
                     NIDKSIWERVIKKSFPEKLIKVNLEAFQMGIHA"
     gene            complement(16689..18452)
                     /gene="iorA"
                     /locus_tag="PLG98_03780"
     CDS             complement(16689..18452)
                     /gene="iorA"
                     /locus_tag="PLG98_03780"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOE32402.1"
                     /translation="MKVLMSGNEAIARGAYEAGIRFATAYPGTPSTEILENFCKYDGV
                     YAEWSTNEKVAVEVAIGAALAGEKAMAVMKHVGVNVAADPVFTVSYTGTNGALVIVTA
                     DDPSLHSSQNEQDNRNYARFAKIPMLEPADAAEAKHYIKMAFEISARFDTPVFFRTTT
                     RVSHSKSVVTLEEPAVYEDKTGFVYNAEKYVMVPHCARIRRAEVEKRQQALKEFAETF
                     PENKVEITNAEAGIITAGMPYNYAKEVFPGYSYLKLGMVYPLPEKMIRDFASKVKKLY
                     VIEELDPFLEEQIKAMGIAVTGKEIFPYTSEFDPGVIRNAIEKQSIKKVSDYKVPLAA
                     RPPNLCPGCPHRGLFYALKKHKVYVHGDIGCYTLSYMKPLEGLHSCICMGASIGMAHG
                     MSKALKEKGKGKVVGVIGDSTFLHSGITPLLDMAYNQSDALIIILDNSTTAMTGMQEH
                     PGTGYTLMGIETKQVKLENLVSVLGIEHVRVVDPIDQKATTSAIKEELTKPGPSVIIA
                     RRPCALFKRSHIKPKPALKVDPDKCEGCRVCIGLNCPPISWKKGATKKGSKRKGVSYI
                     DATLCNGCGLCAQVCKFNAIS"
     gene            complement(18469..19146)
                     /locus_tag="PLG98_03785"
     CDS             complement(18469..19146)
                     /locus_tag="PLG98_03785"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32403.1"
                     /translation="MAEKLTKQDLEGPDAFQNTVELVKDYISENKKRFYSIVTAVILA
                     SMIAFGIYMYWSNYQDSARAMYARTQSSASGSETQDMTGNNIRIFQELIEKYPHSWSA
                     RMAHYRLGNIYYSLGEYDKAIAHHKNFVSSRISDHAGIKFLALSSIGYCYEARKDYKS
                     ALEYFQKAQKGNNIGFESVGFSNVARIYELMNDRKNALENYKKALEKTADSSKAVFIK
                     YKISSLS"
     gene            complement(19139..19570)
                     /locus_tag="PLG98_03790"
     CDS             complement(19139..19570)
                     /locus_tag="PLG98_03790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE32404.1"
                     /translation="MSVNNVTKIRVLFADTDAMGVVYHTNYIKWFELGRNELMRQLGV
                     PYSELTKLNLNLPLIKVGCEYLKFATYDQLLAVETEFAYIRKATVGFNSKIWDEDQKH
                     LLAEGYTVHACTNHEGKLRRIPQLLLELVKKYNINTEEAYG"
     gene            complement(19560..20783)
                     /locus_tag="PLG98_03795"
     CDS             complement(19560..20783)
                     /locus_tag="PLG98_03795"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOE32405.1"
                     /translation="MKYVILLGDGMADYPTDKLGGKTPLECAFTPFMDQIAAEGTLGL
                     VDTVPEGIHPGSDVATLSVLGYDPRENYTGRGPLEAASMGIDLGPDDVAYRCNLVTIG
                     DQDGPEAFMDDFTAGHISSEEAEIIIRDIHRHIGSDHLEFFPGVGYRHLLVWRNAPAN
                     PDTTPPHDITGKPVAEYLPRGNAAADVNAIIRRAADLLKNHPVNAKRRQAGKKEANAI
                     WLWGQGKKPRIVPLTQKYSLSGGMISAVDLLKGIGTIAGLKVCPVQGATGYIDTNYEG
                     KAKMALDVLNFMDFVFVHLEAPDEMGHEGNAEGKIRAIELFDEKIVGPILTKIGAFGH
                     YRIIVLSDHPTPLDLRTHVSDPSPFAVLSSEKKENRAPGMSFNEINAKAGNLLISPGH
                     LLMEKFIKDWKSVVG"
     gene            complement(20818..22128)
                     /locus_tag="PLG98_03800"
     CDS             complement(20818..22128)
                     /locus_tag="PLG98_03800"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOE32406.1"
                     /translation="MKEIPIGLIGFGTIGTGVVKLLRQNEELITKKLGARLVIKKIAD
                     IDITTPREVRVHKGLLTRDAREIINDKDIPIIIELMGGYEPARTFVLEAVANGKHVVT
                     ANKALLATHGNEIFPAAQKNAVNIGFEASVGGTIPIIKTLKESLVANRIISVMGIMNG
                     TCNYILSKMTDDDKPFDMVLKEAQKLGFAEADPTFDIEGIDTAHKMAIILSLAYGRKV
                     NLKDIYLEGITKISREDISFAKELGYRIKLLAIAMLRNNAVEARIHPTMIPSKCLLAN
                     VNQNYNAFHIMGDASGPVFLFGQGAGMMPTASAVFSDVMDSARDVLSGKNGRGPLRSI
                     NESAMMPIRLIPMDHVETKYYFRFTAVDRPGVLSRISGILGKYNISIEACIQKARVKR
                     GAVPIVMTTYKAKESDVQKALKRISKLDILQGETILIRIEDDLN"
     gene            complement(22319..22732)
                     /locus_tag="PLG98_03805"
     CDS             complement(22319..22732)
                     /locus_tag="PLG98_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE32407.1"
                     /translation="MKKLIWVIPSVLFFLFAGCSAGLHSIETVQLAKTVRSWDGETLP
                     QYLQGQPEVTILRIRIPAGSKLEMHHHPVINAGVLLKGELTVVADDNKTLHLRAGDSI
                     VELVNKKHYGKNEGKEAAEIIVFYAGVENKPITVK"
     gene            complement(22778..23710)
                     /locus_tag="PLG98_03810"
     CDS             complement(22778..23710)
                     /locus_tag="PLG98_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOE32408.1"
                     /translation="MTIKVAAIQLATAVAESEENIRRCERLSLQAVNEGAQWIALPEF
                     FNTGVSWNPNIARAIQSADGEAATFLCGFSKKYRVLIGGSFLCRLPDGQVRNRYQCYA
                     QGRLVGQHDKDIPTMWENYFYEGGDPGDIGVLGLYENVRVGAAVCWEFMRTMTARRLQ
                     NKVDVVIGGSCWWSIPTNFPLFLQKLWEPDNKRNALEVLRDNARILGVPVIHAAHCGE
                     IECAMPGLPMSYTGFYEGNAVILDAEGRILAHRKPEEGEGVVSAEILMMPKQDSREIP
                     QGYWLRKRGILPFFAWHHQRWLGRRWYARHVRGN"
     gene            complement(23715..24656)
                     /locus_tag="PLG98_03815"
     CDS             complement(23715..24656)
                     /locus_tag="PLG98_03815"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOE32409.1"
                     /translation="MMGVRIGIIGIGGVGGYFGGKLAREYEKSDEYEIIFIARGEHLK
                     AIQKNGLQLFTREGDCVVRPRIATNNPDEAGMFDVVFFCTKSYSLENSAEAFISCIGR
                     QTVVIPLLNGVNSSQRLRRVLPHAIVLGGSVYIISHSDGPGIIKQEGGACKLTFGTDH
                     DPADDYRYILDILLQAKINAVLTDDISGVLWIKYLLMCPLGSLTSATGKTYGQIWENP
                     ELRQKARAMMMEVVAVAAAHHVVIQEHEVDKAMAMIAGFGYHSRTSMQLDFEKGRQTE
                     IDALTAYLCGAGRDYGVPTPLHDDVYAQLKSIKEPKE"
     gene            24835..27378
                     /locus_tag="PLG98_03820"
     CDS             24835..27378
                     /locus_tag="PLG98_03820"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOE32410.1"
                     /translation="MRIQSFVVHLRFKSAILFASILCIVISGCTPLLNVSYKKSLPPM
                     EGEQVLPGLSDNVTVKRDNLGIPYIEAQSMDDLIFAMGYISACDRFTQMEGFRLVSQG
                     RLSELLGRATLEMDIYLRALNLNQVAQTLYLSASSDLRRLLNIYSDGVNAYLANVPLP
                     TTLKLAGHQPDPWRPIDSMYVFLVLTLGLSQNLQEEINLLNVAQVVAPEKLAWLFPIY
                     PDEPLPFEEMKKLKGINLSEAAQNIEQLGVIANLTNTLLLPAAAASNNWAVSGKLTKS
                     GKPIVANDTHLPLTMPSIWHMMHLKCPEIDGAGIGIAGVPGIVAGYNRHIGIGMTMVM
                     ADNQDIFLEKLQQKPDGLYFLYKNKWLKANSREESFRIKGQKKAVTRTFYETLHGVLL
                     NDILTDKPRHELVPMPLNPSLGIAYSWAGNETDRSMDVFFNIMLAKSVDEILKYTSQE
                     TSVIPLNLVMADRKNIAWQVTGRFPLRKKGRGLCPSPGWTGEYDWNGFLSPSKHPSLK
                     NPAKGYVGTANHRTVPADFPYILSSSWYYPDRGERIEQLLKNAKNYTLDDAKAMQLDT
                     YSRFVNVIKTALLEQKTLDTMSSVWQNSEKSTAAHQAMALLRDFDGNMRLDSAGAAFC
                     GAFLFSLSDNLFADELGGKNTRAYQSLLHTFLLAYSALHDHLTDRGKRSPLWDDVTTP
                     QKEERDLILAKTVLDAIGILEKRCGKNPDKWQWGKLHTYHWRTDATLFADYMDFINKT
                     GVKFLSGYVDRGPYPAPGDHTTLNVSAYHPGKDFGTWMIPAMRIIVDFGSDEPFVGIN
                     STGQLDNPSSPHYDDGITAFRLGQYQNFPFKEENIKTQYTKILTLVPKK"
     gene            complement(27767..30229)
                     /gene="recG"
                     /locus_tag="PLG98_03825"
     CDS             complement(27767..30229)
                     /gene="recG"
                     /locus_tag="PLG98_03825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOE32411.1"
                     /translation="MDALNEILQKIDQPLNFAFRDNYKNLSNIKDLGRTLITLLSKLD
                     SFAASFGREEINHEIGALREIFSDYDRQKLELKKRKIENAVKIVEKLKTIIHFLNISV
                     PERNADQKTIEKISDLKEALEKLNLPVQYLKGVGPKMASRFANKKIATVEDLLFFLPR
                     TYEDRREIRKINRLEAEKVQSAVGNVISCQYRYYGRKKILETVISDSTANLTAKWFKG
                     QMAYLLGVFKKGTRVIFTGDVRPDYVGKQMIHPDYEILDEKDEENLLNFKRIVPVYSE
                     TEGLYQKYYRKVMNAALMNYSRYVASPIPDSICEKRHLLNVHEALWSVHFPGSQEDID
                     QFISSRSEAHRRLIYDEFFFFQLGMAVKKSGRILDQGLSFKTDGTMLDKFYSSLKFDL
                     TAAQKRVIEEILNDMSKDTAMNRLLQGDVGSGKTIVSMAAMITACENSYQAAIMAPTE
                     ILAKQHFENIRLWAEPLGLKTVLLTGSMANASRGETLRRIQNGEAGIIIGTHALIQEG
                     VDFHKLGLVVIDEQHRFGVIQRATLRSKGINADVLVMTATPIPRTLAMTVYGDLDVSV
                     INEMPPGKKPVHTVVLPESKRDKVYQAIRAELAKGHQAFIVYPLVEESETLDLKDATN
                     MSRHLQQDVFPDYRVGLIHGKMKEKEKDEIMREFMENKIHILVSTTVIEVGIDVPRAS
                     LMVIEHAERFGLSQLHQLRGRVGRRDILSSCILLANYKCSADARKRLKVMETTTDGFT
                     IAEEDLAIRGPGDFLGTRQSGLPDFRIASIIRDARILSEAKEDAFALAARDPFLDRVE
                     HQILKEVLIWKWQGKLDLARTG"
     gene            30405..31454
                     /gene="purM"
                     /locus_tag="PLG98_03830"
     CDS             30405..31454
                     /gene="purM"
                     /locus_tag="PLG98_03830"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOE32412.1"
                     /translation="MTDNITYKDAGVDIDIANEFVERIKTSVKTTARKEVVSGIGGFG
                     GLFRLNSVDLKNPVLVSSTDGVGTKLRIAHLLDKHDTIGIDLVAMSVNDVVVQGAEPL
                     FFLDYLATGKIELEKSVSIIEGITEGCRQAGCTLLGGETAEMPGFYAPGEYDLAGFCV
                     GVVESDKLITGSTISVNDRIIGLASSGLHSNGFSLARKVLLEKGKLNVNDKLPGLEKT
                     VGLEMLEPTRIYVKALLNLFKKFTLKGLVHVTGGGFYENIPRIIPQACRAVIGKGSWP
                     VPPIFNIIREIGNIDEKEMFRVFNMGIGMMIIVSDKDCPAVLEYLEILGEKAYPLGLI
                     EIREDKKPQVIISES"
     gene            31464..32078
                     /gene="purN"
                     /locus_tag="PLG98_03835"
     CDS             31464..32078
                     /gene="purN"
                     /locus_tag="PLG98_03835"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOE32413.1"
                     /translation="MADALKLGILISGNGSNLQSIIDHIEQGSLRAVIQIVISNNPDA
                     YGIIRAKKHGLPFCVLKNSDFKNKEEFDSELINTLIKHDVELVTLAGFMRIISPRFLQ
                     AFPGRIMNIHPALLPSFPGLHGQRQAVTYGVKVSGCTVHFVDEGVDTGPIIIQRAVPV
                     RDDDTEDTLAARILKEEHKIYPQAIQLFADGKLEIRGRKVFIHP"
     gene            32144..33580
                     /locus_tag="PLG98_03840"
     CDS             32144..33580
                     /locus_tag="PLG98_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32414.1"
                     /translation="MYDLIVIGDDLSSHVAAAFCAINGLKTALFSEKGTAGSSVAGDL
                     TFDGTITPVSGFAENQIAYLSLKNLGIYPEVRLLNPAYQIILPSQRIDFFHDKEELLK
                     ELAREFPHLEGDLRLFYDVAEKNSNAAGQWISEHPGIQPKKMKDFFDYLKLIPRFVES
                     MFNTIRLKRLMSQNPSFRKVIQAQHALLSFKTDPLNSFFSSWQSCTPLRGTFSFAQGK
                     QDLVDSLIRKIKASDGLYLSGCDCLSIHKDKVIELNYTDDNGNTEKIESRHLIVSTKW
                     QNIGQLFDHKKTFSFGDFLRPAKTAYFPFSIFLGVNPAGLPEKLARHSAIICNPAREL
                     YAHNNLIILESSAKEERMKDPAAKILLSATVFLPDHQEIWSKDNLKTISENIMNQLDH
                     FFPFLKENILFFDPDESMKLSLKLRGTIIPRYQIKNSFFTGFSAKGSKTRYANIYLTG
                     ASLLADAGFEAEILSGLNTANLLISQRK"
     gene            33584..34882
                     /locus_tag="PLG98_03845"
     CDS             33584..34882
                     /locus_tag="PLG98_03845"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOE32415.1"
                     /translation="MKDVFSISNLGKRMIVSPLQTGKFTRDGKRALFNTHLDNYRSLT
                     DKKGQPLSVELAGPREKIFFNPADTRAAIVTCGGLCPGINDVIRSITTALYFNYHVKK
                     IVGVKYGLQGLNPHCGHPLISLSPDVVKDISSFGGSILSTSRGPQDVQIIVDYLHRLN
                     INILFCIGGDGTIRAAEKITAEITRRNFKIAVIGIPKTIDNDLHLIQKSFGFDTAIEK
                     AADAIKSAHVEARGMVNGIGLIKIMGRHSGHIAAHAALAQSDANFVLIPEVPFDLEGK
                     NGFLGFLKKRMISRGHAVIIVAEGAGQDLLGTTDRSAEKDASGNARLLDIGSHLKNVI
                     ENYFKKISMEINLKYIEPSYLIRSVPANASDDIYCNMLGQYAVHAGMAGKTGILVGLM
                     KDEYVHLPLAAVASGRKIDPRGDIWLRVLETTGQPASMKN"
     gene            35064..35261
                     /gene="rpmB"
                     /locus_tag="PLG98_03850"
     CDS             35064..35261
                     /gene="rpmB"
                     /locus_tag="PLG98_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOE32416.1"
                     /translation="MSRVCEVCGKKPSVGNAVSHANNKKKAVWYPNLQNLRCVDEKTG
                     SIKRIKVCTRCIRSGFVKKAT"
     gene            complement(35254..36042)
                     /locus_tag="PLG98_03855"
     CDS             complement(35254..36042)
                     /locus_tag="PLG98_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32417.1"
                     /translation="MLTHTYFLQKILGSAEIKNVEPDIFVYNIAPDLLAIHPDITSAR
                     THKLRRLQTLPVEYPKAAYVIYHLLIDDLSHYGYICTEDGDAFDIHSRGYSYRKGNPL
                     IRVIQDIHGIAGKEISCRDAVYQSHLIIEMIYDLVIVRHLRTFKTVEVMVDAIKYTVE
                     NRMEEFTETLRWLYGLEVNQIREVMRSALLFITQESMEQMMTIQGRMHLYQDKFGLPC
                     NDLLLNNELNRLFEQAMDLIDDDELFFNETADVIKESQIISFIR"
     gene            complement(36072..36293)
                     /locus_tag="PLG98_03860"
     CDS             complement(36072..36293)
                     /locus_tag="PLG98_03860"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HOE32418.1"
                     /translation="MMSKYFQLDRKNIVAVQFIIEGYEGLATVTTIDAAVAVIQISIM
                     PDFLREMNCLLDELSKKYHMKELVDDRIR"
     gene            36377..38338
                     /locus_tag="PLG98_03865"
     CDS             36377..38338
                     /locus_tag="PLG98_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HOE32419.1"
                     /translation="MRLVRNTSILLLLFIVSCSPIHPFIKSISESDVASFLQNAPRAE
                     EHPNAGADLLYSYSYVEFFEDGRSISRNLDRIKIFNERGRSFASKSISYREGYQKVKI
                     IFANTIKPDGKIIALDRKDIIDSSEYAGYEFYTDIKVKKFTMPAVEDGCIIEIAYEIE
                     NLKPVLSMDYSETFFCQNFFPIEEDIMEIVLPAGTKLQYENFRTDITPQITSLGNKIK
                     YVFINKKQKEIIPESRMPSLLDRDTFPQISFWTLSNWDIISQWYIKLFKEQMKSSAEL
                     ENFTRELIADQKTDEDKINAIFNFVSQNIRYIAVLLGPHTHKPHQAFEVFEKRYGDCK
                     DKTVLLLTMLKIASIQGLPALVPANGKYFDETIPSMNAFNHIIAVVPLKGKSFWLDAT
                     NETSSYDSPPFMLPTRIFLINDDGSYRFVTTPPLNDKKDYYHLAIRYKIDTEGNAAID
                     YVYNFFGKAAEAIRYSFKYAPPEQRKKFFENRGIEIRDLELGSFSDTRTPFTIKLSGM
                     IRNLAQVLDEETMVLSNIIPLDAYRDITAASIRRYPISLTQSMYAMENNTYQFPPGFK
                     IKKLPQNYIFKKPFRYRKEKYSFTGSEFRVYLETKNEEQNIPLGKMNEFKKYASELQK
                     HESAVKNIIFYKRYQNLDTRKDIDIINHK"
     gene            38358..40097
                     /gene="recJ"
                     /locus_tag="PLG98_03870"
     CDS             38358..40097
                     /gene="recJ"
                     /locus_tag="PLG98_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOE32420.1"
                     /translation="MTTQRKLPSLKWKIEEAGNKNDGALLSKEFGIHPIIAQILLNRG
                     IRDVDKARHYLQPSLNDLHSPFLMKDMRQGVARLLKAIHNNEKIMIYGDYDADGITSV
                     VILYKFIREITPVLDYYIPDRIQEGYGLKESAINEFKKNGVSLIITVDCGISDVEQIA
                     YARSLGIDTIVLDHHEISGGLPPAAAAINPNRDDCPYPFKGLAGVGIAFNFLIALRGT
                     LNKEGFWKDNHYPNLREYLDIVTLGTIGDIAPLTDENRIFTKIGLELITEGRRPGIKA
                     LKEVSGVDNQVIDSFKASFSLIPRINAAGRIASPSDAVKLLLTDDLSEARPLAEKLDS
                     YNRHRQLMEKKILSDILEMIGKNRSLEKMNSLVFASDKWHPGVIGIVASRLVELFNRP
                     AFVISIINGVGKGSGRSISGFNIYKGIQQCEHLLLSFGGHYHAAGISIEEANIEKFSA
                     VLDELTGDIAEFSEIASQITIDTECGLEDIDPALLAQMEALAPFGSRNPEPLLLAHNV
                     KASSLVVVGNNHLKMSINSNGTSRDSIWFNMGKHLSALDGASLDIVFTPQINNWNGSA
                     DIQLKIKDVNIVS"
     gene            40126..40515
                     /locus_tag="PLG98_03875"
     CDS             40126..40515
                     /locus_tag="PLG98_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32421.1"
                     /translation="MMNVATKAALFNALVFPGWGEIYLKKYRRGLLIITGMAAGILSI
                     LLLVVQETLAVLKVTPVDKSTLSLGRLFHLSVKVMHSLNPSCILIILFFMIFLWLLSI
                     IDAYRLGKETMLEISTDADQQSASPEA"
BASE COUNT        10949 a         9751 c         9278 g        10588 t
ORIGIN      
        1 catcctgacg cggtggatgt ccagggttca aatccctgct tgcctaccat ttttatataa
       61 attacgtctt gatttcataa taacaaaggg catcagcaag acagtgatgc ccttttaaaa
      121 tgaagttaag gtgctttgac gaatgatatg actataaaaa ttacttttcc agatcatcag
      181 gaattatcct tcaaccggtc aacgccggcg gctgaagccg tcagtgcctg gaaaaaggaa
      241 gctctggccc ataccgtggc cgtaaaaatc aacggcatcc cggctgatct cagccgtccg
      301 cttgaccgag acgccacaat tgatctcatc gacctgtcct ccgctgaagg gttgtctatc
      361 ttgaggcaca gtatatctca cgtcatggct caggctgttc aggatgtatt tccgggtgcg
      421 aaagtgagta tcggcccgtc catcgaagac ggcttttact acgattttga atatcatgaa
      481 accttcacga cggatgattt tccgaaaatc gaaaaacgga tgaaagaaat cacaaaggcg
      541 gataatcctt ttgtccggga agaactgtcc cgtgaagcag ccgttgcgtt attcgaggaa
      601 agagaagaaa attacaaagt tgaattaatc aacgatctgt ctcatgacgt caagacggtc
      661 agcctttata aacagggcgg ctatgttgat ttatgccgcg gcccccacat cccgtcaacg
      721 ggaatgatca aagccttcaa gctgctcagc gttgccggag cctactggcg aggcaatgaa
      781 aacaacaaaa tgctgcagcg tatctacgga actggttttg ctactgaaaa tgagcttcag
      841 ggatatttga ccctgctgga agaagcccgt aaaagagatc atcggcgtct gggcagggaa
      901 ctggatctgt ttcaggtcaa cgatgaagcc ggagcaggcc tgattatttt ccatcccaag
      961 gggatgctgc tgcgttatct gattgaggaa tgggaaagaa aggaacatct caaacgcgga
     1021 tacgatatgg tcatgggacc gcagatcctc aaggtggatc tctggaaaaa atccgggcat
     1081 tttgaccatt accgggaaaa tatgtatttt actgaagtgg atgagcaggt ttacggtatc
     1141 aagccgatga actgtctgtc ccatatgctg atctataaat caaaaatcag aagttatcgg
     1201 gatttgccgc tgcgttattt tgaactgggc acggtgcatc gtcatgaaaa gacgggggtt
     1261 ctgcacggtt tgctgagagt gcgacaattc acccaggatg atgctcatat tttatgcaca
     1321 cccgaacagc tgaattcgga aatccggggc attgccgatt ttgtcagcca tgccatggga
     1381 attttcggat ttgaatatga ggtggaatta agcacccggc cggaaaaatc aatcggcaca
     1441 gacgccgatt gggatctggc cacctctgcg ctggaaaacg cgctcaagga caatcacatt
     1501 gcctatgatg tgaatgccgg cgacggtgct ttctacggcc cgaagatcga tttcaagctc
     1561 aaagacgccc tgaaaagaaa atggcagtgc gccacgatcc aatgcgactt cacgcttccg
     1621 gaaagattcg atttaagcta tatcggaccg gacggagaaa agcatcgtcc ggtcatgctg
     1681 caccgggtga ttctcggcgc gattgagcgt ttcatggggg tgctcatcga acattacgcc
     1741 ggcgcgtttc ccgtctggct gtcgccgacc cagtgcgttt tgctgacggt caccgacaag
     1801 catatccctt acgctcagac agtgcatgat aagctgatcg catccggcat ccgctccgag
     1861 cgatattttg acaatgagaa actggggtat aagatacgac aggctcagct gcagaaaatt
     1921 ccctatatgc ttgttatcgg cgacaaggaa atggacgcgg gcgccgttgc accccgcaca
     1981 cgcgacggaa agaacatggg cgccatgccc gttgatcagt tcattacatt agtacatcaa
     2041 acttgtgatc aaaataaata ataaacgaca ttcggataag ataaatttaa tttcaggagg
     2101 tgacctatag taaaggattt aagaatcaac agagaaatca aatcggccaa tgttcgcgtc
     2161 atcagtgaag acggtaaaca gttgggcgtt atttccctgg aagaggcact tgccaaggcg
     2221 gaagaagccg gtctggattt ggtggaggtt tccgcatctg ccgatacccc ggtctgtaaa
     2281 ataatggatt acgggaagta tcgctacaag cagaacaaga aactgcatga tgccaaaaag
     2341 agccagaccg taattcaggt taaggaaatt aaattaaaac cgaaaacgga agaacatgat
     2401 gtgcaggtca agatgaaaca catcaaaaag tttttgaaga atcatgataa agtaaaggtc
     2461 tccatgatgt tcagaggcag agaaatcgca tttaccgata tcggcagaaa gctgatggga
     2521 aaaatacggg atgaactcgc caatgagtgt atcatcgatc aggagccccg tctggaagga
     2581 cgcaatatgg tgatgattct ttcaccaaaa aaacaataaa tatggaaagg tgatcacatg
     2641 ccaaaattaa aaacacaccg aggagctgca aaacgctttt cactgaccgc caaagggaag
     2701 attaaacgca gcaaagcgtt tgccagtcac atcctcacga aaaaaacgac aaagagaaaa
     2761 aggacgttaa gaaaatcaac gattgttgat tcggcaaacg caagagaaat taaaaaactt
     2821 attccctaca tgtaatggac cggggataat ttacgagtta tctgtaaatg aggagaaaaa
     2881 gaaatgtcca ggataaaaag gagcgtcaac gctcgtaaaa gaagaaaaaa ggttttgaaa
     2941 ctggccaaag ggttctttgg cgcccgcagc cgcttgcttc gaacggctac ggaagcggtt
     3001 aacaaggcga tgaaatacgc gtaccgtgac cgccgggcga gaaagcggga attccgccag
     3061 ttatggattg cgcgcatcaa tgccgccgca agcgataaca acattacgta cagccgcctg
     3121 atcgacggga tgaaaaaatc cggtattgaa ctggatcgta aaattctggc ggaactggcc
     3181 gtcaatgatc ctcagggatt tgcgcaggtc gtgaccattg ccaaaggcaa gtaaactact
     3241 gctatgatga aagagcttca acaactggaa agcgatgctt tgtcggaatt aagtaccgcc
     3301 acgtccgagg agcaggtctt gactgtcaag acgaaatatc tgggcagaaa aggccttcta
     3361 accggtcttt taagaaacat ctcccgggtg cctgatgaag aaaagccgct tttcggtaaa
     3421 cgctgcaacg agttgaaaaa ttctctgaat gccaggcttg atcaggccat tgagcagtac
     3481 acaagccgga ataaagaaca agcgctcttc aaggaaaaaa ttgatgtcac gctgccggga
     3541 cgatccgtca actgcggacg tgtgcatcct gtggttcaga tccgcagaga aatctgttcc
     3601 gttttctcgt cattcggttt ttccatcgtg gaaggaccgg aaatcgaact ggattattat
     3661 aattttgaag cactgaatat accgaaagat catcccgcgc gggacatgca ggataccttt
     3721 tatattgatg agaacatcgt tttaaggacg cacacgtcgc ctgttcaaat cagggtcatg
     3781 gaaaagatca agccgccgtt gagaatctta tctcccggaa aagtgtaccg ccgcgattcc
     3841 gatgtgtcgc acacgccgat gtttcatcag atcgaaggtc tgctggtaga taaaaacgtg
     3901 accttcggcg atctgaaggg aatcctgtcg gcgttcttga agaagatctt cggcagttca
     3961 accatgctgc gtttccgccc cagttttttc ccgtttacgg aaccgtctgc ggaagtggac
     4021 atctgctgtg tgatgtgcgg cggcaaaggc tgcagggttt gcggacaaag cggctggctg
     4081 gaaattctcg gatcgggcat ggtcgatccg gcggttttca gaaatgtcga ctatgatccg
     4141 gacgaatatt ccggatttgc ttttggactg gggctggaac gtattgccat gttgaaatac
     4201 ggcatttccg atatccgttt atttttcgaa aacgatatcc gtttcctcaa acaattttag
     4261 gagatttttt ctttatgctg gtcggtctca cgtggcttaa agattatgtc gatgtgcaat
     4321 taacggcgaa ggacctggcc gacaaactga cgatggccgg gctggaagtg gacgaaataa
     4381 aaaaagtcgg tccgtccttc agcggcgttg tggtggcaaa aatcatatcg gtcggcccgc
     4441 atccgtccgc agagcgtctg tctttgtgcg atgtgcatga cggaaaggac ctttatcgga
     4501 tcgtctgtgg agccaaaaat attgcaccgg gaaatatcgt tccgctggcg aaagtcggcg
     4561 cggtgattcc gggaggctat acgatcaaat cctcggttct tcgcggtgaa aaatcggacg
     4621 gcatgctctg ttccgaagcc gaactggaaa taggaagcga tgccacgggc attatgcaac
     4681 tgtccccgga tcttgtactc ggtcaaccgt tggaagaggc tctgaacctt gaggatacgg
     4741 ttttggatgt caacgtaacg cccaatcgtc cggattgtct gagcatgatc ggcatcgcca
     4801 gagaagtcgc cgccctgacc ggaagaaaaa tcaaatggcc tgctgtccgg attcacgaaa
     4861 aggctccgga catccattcc ctaacctccg taaaaattat cgattccgat ctttgcccgc
     4921 gttacaccgc ccgcgtgatc aaaaatatcg ccatcgctcc gtcgcccaga tggatgaggc
     4981 ttcgtctgga gtccgcagga ctgcggtcga tcaacaatat cgtggatatc accaattttg
     5041 taatgctgga aacgggtcag ccgctgcacg cgtttgattt ccgttttctg gaagagggaa
     5101 gaatcgttgt ccggcggtcc cttgaaaacg aagaatttat ttcgcttgac ggcaagatac
     5161 gcacgcttcc cgccgatacc gttttaatct gcgacggcat caaaccggtg gccattggcg
     5221 gcattatggg ggggctgaat tccgaagtca aagaagacac gcaaacggtg cttttggaaa
     5281 gtgcgtattt tcgtccgacc tcgattcgtt cttcctcgag aaaactgggc atgtcgacgg
     5341 atgctgcatt tcgttttgaa cggggaatcg atcccgacgg tgtggtcaga gcccttgacc
     5401 gtgccgccca gcttatggtc gatctgtccg gaggttctat ctgtaaaaat tatatcgatg
     5461 aatatcctcg gaaaatttct tcagccggag atattccttt gcggctcgac aggatccgca
     5521 aaattatcgg cacaaaaatc gccgcccggg atgttttgaa aatattacgg cggctcgaca
     5581 tgacggtgaa accggaaggg aagggcagat atctggtgac gcctccgacc tgcagggtag
     5641 atcttgaaag agaaatcgat ctgattgagg aaatcgtcag actctacggt tacgatcgag
     5701 tacctgttac gctgcccgac gtatccgtca acgaactgga cgttattgcc cgtctggata
     5761 tggaagaaag acttcgtcaa ctgttgacgg gaagtgggtt tacggaaatt gtcaattata
     5821 gtttcggtac accctctgcc gcggatatgc tttccctgcc cgcaacagat gaaagacgaa
     5881 atacagtcag aatcaaaaat ccgctgggag aagagctctc cacgatgcgg acaaatctga
     5941 tttacgggtt gctggatacg gccaaaagaa atttcaataa cggcgcattc gatttgaaac
     6001 tttttgaaat cggccggata tttttgcatc gacgggaagg ggaattgccc gaagagaaaa
     6061 ccatgattgc gggactcatc acgggaaaga tcagagatga tttctggaac tccgaacaga
     6121 cggtggactt ttatacttta aaaggagctc tggaaaatat cttttgtgac ttcaagattc
     6181 agaactgccg ctacgcagca acgacggacg agtccttttt gcatccggga aaagcctgtc
     6241 acatagtttt atatgaaacc gtaatcggtg ctgtcggaga aatacatccg gatgttctgg
     6301 aaaaactgga tatccgcaat acaatttatg tatttgaaat gaatatcgat attctcagca
     6361 gggtttgtga gcaaaacagc ctatccttta acgaagtgtc gaaatttccg gctgttatcc
     6421 gcgatgctgc gtttgtcatt cccgatccga tggaagcaga gcaaatgata gacattgtct
     6481 tgagccagat ggaagaattg cttgaaaatg taagtatttt tgatgtatat aagggcaaag
     6541 gaattccgga aggaacaaag agtctgggat tgcgattttc ttaccgcgcc aacgacagaa
     6601 cattgactga cgccgaagca agtgccgttc acgacagaat cgtgcagaaa accgtcaatg
     6661 tgaccggtgc aaaactaaga tcataaaata ttcactaagt aaataaaatg gaggtaaaat
     6721 atgacgaaga ttgatattat tcagaatgtt catgagaaac tcggtttttc caaaaaagat
     6781 tcttcagata tcgttgaagc ggtttttgat cttatcaagg attgtctggc caatggcgaa
     6841 aaagtcaaga tatccggctt cggcaatttt atggtcaagg agaagagagc ccgccgcgga
     6901 cgtaatcctc agacagggca ggaaatttcc attacggcaa gaagggtgct gacgttcaaa
     6961 tcctcacagg tgctgcgcaa agccctgaat taaatttgga cattagccgg tccggttgat
     7021 ttcataagca cggcgtattt gaaataattt tttggtttta cttcagcctt aaagactttg
     7081 agaacgttat ggaaacgatc attcccgata aagcttattt tcgcatcggc gaagtaagca
     7141 aaattctcaa tgtggaaccc tatgtcatcc ggtactggga gactgaattc aaaacagtca
     7201 atcccatgag aacgaaaacc gctcaaaggc tgtacagaaa aaaggatgtg caggaattgc
     7261 tgaccattaa aaatcttctc tacgcacaac gcttcaccat tgacggcgca aaaaaacagc
     7321 ttcaaaaatt acgcaatacg gatgagcctg aagagaaaaa cagcgaaaaa caaaaactct
     7381 tcttgatacg gatcaaacag gaattacaac acatcaggga gattgtcagt taatataaaa
     7441 aagccggctt tcaaaagccg gcttttttca tcttttcgtt tttaagcttt tttgctggcg
     7501 cgtttggagg ccttgagcgg attaatcttt tgagcttctt tgatttcaat ttttacgtgc
     7561 gtagctgtcg ggcaccttcc aatcagccac ttccctgcag gatcgggata aaccttacaa
     7621 tatctggccg attcatattc aaatattttt ccgcaacctt cacattgttc aacaacaacc
     7681 tgacaactgc cgttggtata actgcagccg tttttagtca taaaaacgca gtcaaagccc
     7741 gctttaaccg tcttgcaaat catggatgca tcccccttat ataattcgag tatttgacgt
     7801 tacggggaaa aactatcaag gatattttca ccaacgtctc ggttatttaa agtttgtgtc
     7861 cttaacaaca aaaattatag ctgtcaagta aaatgaattt cctgaaataa aaagaagaag
     7921 ggttttcttt ccagaaataa cttgaatgaa taacgattta ttgataaata tataaaaatt
     7981 gtttacggaa gagtcaatgc gagcagtcat tcagagagtt gaccgcgcct gtgtcagcat
     8041 taatcgtcag gtattctcct ccatcaacga aggaattctt atattactgg gtgttgagaa
     8101 atcggacacc gaggcggatg ccgattatat tctggaaaaa gccgtcaacc tcagaatttt
     8161 tgaagatgat caggacaaaa tgaacctctc tcttctcgac acccaaggag acatgatggt
     8221 tgtctcgcag ttcaccttgc tggggaattg cgtcaaagga aggcggcctt cgtttgacgc
     8281 tgcggaagaa cctgttcaag cctgcaggct ctatgactat tttgttcgtc aggcgaaaac
     8341 attcattccc ggtctggcca cgggaaaatt tcaggaaatg atgaaaatag acctggtcaa
     8401 cgatggtccg gtgaccatca tactggacag cagaaagatg ttttaaaaat ggataatttc
     8461 gaaataagaa tagacaaaga cggtctctgg tattataaag gcgcgcacat gtttcgcaag
     8521 gatattttga gtatctttta cgaacatctc cggattgatg attcgggccg gtatctgatt
     8581 aagctcggtc cggaatgttg ttatctggcc gtggaagata cggtgtttgt tgtcctggcc
     8641 gttttcaaaa caaaaaatgc ggacaaccgc cgggatcaga tcgaaattct gttaaacgat
     8701 gatacgtgcg aaatcctcga catgaacagt ctgcggtcag gcaaggaaaa cgtgctgtac
     8761 tgccgtgtta agatcggaaa attcaccgcc cgattctcgc gcaaggcgta ttatcaactg
     8821 gctgaattca tcgaatcatc ggaagacggc agccgtttct ttattcccct gaacggtcaa
     8881 aaatatttta tccgtcaagt tcaggattcg gacgcctgcc cgacaaggcc atcctgatga
     8941 ttaaatggtg ctgagacaga cggaccatga tgacgccaca ggcattctgc agcgttacgg
     9001 ggaataaaat tttctcacaa gagcgttgag gctctatatt attaacaagc aaccgggatt
     9061 tattatcaat ggagggaaaa atgctcgatg acacaattaa ggaatcttta acgtttgacg
     9121 atgttctgct ggtgccggca gcgtccaata tactgccgaa agatgtcgat acaaccacat
     9181 cgttaaccaa aaacatatct ttaaaaattc ccatcgtttc ggcggccatg gacacggtga
     9241 ccgaatcgcg cacagcaatt tgcatggccc gtgaaggcgg catcggcatc atccaccgga
     9301 atatgagtat tgaacagcag gccattgaag tggataaagt caagaaatcg gaaagcggca
     9361 tgattgtcga tcccatcacc attgatccgg accggaaagt gagcgatgct ttgaaactca
     9421 tgaatcagta ttcgatttcc ggcgtcccgg tcgtgaagaa aggaaaactg gtaggcatcc
     9481 tcaccaatcg ggatctccgt tttgaagaaa atctggatca accggtatcg aatgtcatga
     9541 cgaaaaaaaa tcttgtcacg gtatcgtcca atatttctct ggaaaaatcc aaaaaacttc
     9601 tgcatgaaca ccgcatcgag aaactgctgg ttgttgatga tgaataccga ttgaagggat
     9661 tgatcaccat caaggacatt gaaaagatca gacgctatcc caatgcctgt aaagattcgc
     9721 tcggccgatt aagggtcggt gccgcagtag ggatcatcga tcgcgaagcc agagttgaag
     9781 ctttgctggc cgcaggcgtc gacgttattg cgattgacac gtcgcacgga cattccgcag
     9841 gtgtcatcaa ggccgtgaaa gcgaccaaat ccgattttcc caaatgtgaa ctgatcgccg
     9901 gtaatgtggc gacggcggac ggggcgaagg cattgatgga tgccggcgtg gacgctgtga
     9961 aagtcggcgt tggaccgggg tccatctgca ccacgcgtat cattgccgga gtgggtgttg
    10021 cacagatgtc tgcaatcaga gacgcctaca aaatagcctc gaaaaaaggg gttccggtca
    10081 ttgccgatgg cggcatcaaa tattccggag atatcgtgaa agccatcggt gccggcgctc
    10141 actgtgtcat gatcggcgga ttatttgccg gcacggagga aagtcctggc gaaaccattc
    10201 tgtttcaggg aagaagttat aaagtgtacc ggggcatggg atcgcttgaa gccatgaaaa
    10261 tgggtagtcg tgaccgctat tatctcgacg atattgaagc gcccttaaaa acggttccgg
    10321 aaggcataga agggcgtgtg ccgttccgcg gttccttatc ggcgagtatt cttcagttga
    10381 tcggcggcct gaaagcgggc atgggatacg tcggttgcag aaccattccg gacatgatta
    10441 aaaaagcgcg ttttgtaaaa attacgcagg ccggtcttag agaaagccat gtccatgacg
    10501 tgatcatcac taaggaagcg cccaactact ggttggatta agtcagttgc tttctgcttt
    10561 ttttattttt tcattatttc ccaagtacgg caattatgaa acactctcag tttgtgcatc
    10621 ttcatcttca tacgcaatac agtcttctgg acgggatgat ccgtttggat gagcttttca
    10681 aaaaagttaa agcatatgcc atgcccgccg ttgccattac ggatcacggg aacatgttcg
    10741 gagcgataga tttttatcaa caggcgttta aaaacgggat taaacccatc atcggcactg
    10801 aactttacgt cgccccgaaa gaccgtaaag acaaaacccc gtcgtccatc ggcgaatcaa
    10861 cgcgccatct ggttgttcta gtcaaaaaca agcaggggta taaaaacctg attaaattaa
    10921 actcgctcgc acatattgaa ggattttact accgtccccg cgtggataag gccctgctgc
    10981 gggaatgtgc ggacggcctg attgcttcga gcgcctgcct gcatggagag atcgcctcat
    11041 ggcttgtccg gggaaacatg gaagaggcga gaagagccgc ccgggaatac ctggaaattt
    11101 tcggagaaga gaatttttat ctggaaatca tggaaaacgg aattcccgag caaaagatcg
    11161 ccaatcaggg gctgattgag ctgagcaagg aactttccat tccgttgatt gccaccaatg
    11221 actgtcatta tctggaacgg gaccatgcgg aagcccacaa tgtgctttta tgcgtccaaa
    11281 ccggcaaaac cattgaagac aaagatcgca tgaccatggc aacggatcag ttttatgtcc
    11341 gatcaccgga ggaaatgtgc gagcttttcg agtctgttcc cgaagcgctg gaaaacacgg
    11401 tcaaaatagc cgagcgatgc aacatgactt tcgaattcgg gaaattctat ctgccgaaat
    11461 ttgaagtgaa gcatccggag gaaaccctgg aggcttatct ggagcgcaag gccgttgaag
    11521 gtcttgaaaa acgcatgccg agcatcctga aatattcaac gggagaggaa aacagcatcc
    11581 gcaaaaaata tagcaagcgt ctccacgaag agctggaaat catcaaaaaa atggggtttg
    11641 ccggatattt tctcatcgtt tctgatttta ttaaacatgc caagcacaac gatattcccg
    11701 tcggtccggg ccgggggtct gcggcaggat ctctcgtggc ttttgccatc cggattacgg
    11761 atatcgatcc tcttcggtat ggcctattct ttgaacgatt cctgaatccg gaccgcatca
    11821 gtatgccgga tatcgatatt gatttctgtc aggaacgccg gggcgaagtt ataaaatatg
    11881 tgacggaaaa atacggaaag gacaaggtaa cccagatatg cacattcggt aaaatgctgg
    11941 ccaaaggtgt gatccgggat gtcggccgtg ccttgaacat tccttacggc gacgcagacc
    12001 ggattgccaa actggttccc tctattccgc ccaacattac cctgaacgaa gccttcaaaa
    12061 tggagccccg ttttgaggaa gaacggaaaa agaatccgca aatcgacaaa ctcttgaaat
    12121 tatcactcat tctggaaggc ttgaaccgtc attcttccgt acatgccgca ggtgtggtga
    12181 tctcggacgc tccgctgatg gaacgagtcc cactcttttc cccgaaggac gatatcgttt
    12241 ctcaatacac catgaaagac attgaaacgg tcgggctgac gaaattcgat ttcctgggct
    12301 tgaaaacatt aaccgttatc aagaacactc tccagttcat ccgcgactat aaagacacgg
    12361 aggtggatat taacaacctg ccgctggatg atcccgacac atacaaactg ctcatgaagg
    12421 gagaaaccga cggtgttttt cagctggaaa gcgaggggat gaaggatctg ctgatcaatt
    12481 tcaaacccga tcgcatcgaa gacgtgatcg cgctgattgc cgcgtatcgt ccgggtccca
    12541 tgaagatgat cccggatttt attgcccgca agcacggcaa acagcaaatc acatatgagc
    12601 ttccgcagct tgagccgatc ctgaaagaaa cgtacggcat tattctttat caggagcagg
    12661 tcatgcagat tgccaatgcg atcggcggtt acacgatgtc acaggcggat accctgcgca
    12721 aggtgatggg aaagaaaaaa gtcgcggata tggaaaaaga aaaaccgcgg tttatggaag
    12781 gcgccaaaaa acagaagatc gacgataaca aggccagaat tatctgggat cagatggaaa
    12841 aattcgccga atacggtttt aataaatccc acagcgccgc ctatgcaatg atcacgtatc
    12901 agacagccta tctcaaggcc cattatccgg tcgaatttat ggccgctttg ttgacgagcg
    12961 aaaaagacaa ccgggacaaa atcattaaac acatgacgag ctgcaaggac atgggcatca
    13021 acatcctgcc gcctgacatt aatgaatcgg aaaaggggtt cagtatttcc ggtgagaata
    13081 ttcgcttcgg tctgaccgcc gttaagaatg tcggggaggc ggcgattgaa tctgtcatcg
    13141 acgtgcgaga aacagcaaaa ttcctatcct ttattgattt tctcaatcgt gtcgatctgc
    13201 gtaaagtcaa caagcgggtc attgaaagcc tgatcaaatg cggcgcattc gattccatgg
    13261 gacataaacg cagtcaatta atgcatagtt atgaagaagc gatggaggtt gcccaacgca
    13321 accagaagga gaaatcaagc aaccagtcaa gtttctttga tcagctggaa tccggctcat
    13381 catcttttga aaacggatta agcgcttatg aacttcctga catgccggaa tgggatcaca
    13441 aaaagctgct gtccctcgaa agagaagcgc tgggttttta tatcacggga cacccattgc
    13501 ttccgtatac tgatcgtctt aaactggtta cagacagcga ttcggaaaat ctgctgatcc
    13561 gtcgcgacaa ggaaaccgtg accgttgccg gagtggtcag cgccataaac gaacggaaaa
    13621 caaaacgaaa cgacaccatg tcgtatgtca ctctggaaga tttgcatgga tcaataaacg
    13681 ctatattttt tggtgattta tacaagacat cctataatat tcttcatgaa gaagaaccgg
    13741 tgattgttaa aggaactctt gatatatccg gcactgaaga aaaccagaaa atttctttaa
    13801 ttgcccaaga agtgactccc ctggcggaag ccttgaaaaa tccctacaaa caggttcgtt
    13861 ttatggttga cgccgacagg atgtccgctg aagatatttc atcctttatc tcttcaattc
    13921 ggaaatttca gggaaaatat gaaagttata tgcatattat gaacggaaaa agtgaaacga
    13981 tcgtttatct gggagatgat ttgcgcctgg atattaatga gaggttgaaa aaagaggcgg
    14041 ataatatact ggggcagggt gctacaattt tttcatgagg ggattgacgt cagatgccgc
    14101 catacgagga gagaacatac agatcgctca taaagaacga tcatctcatt tcttataatg
    14161 taaaaatttc cgaaagcgat ctgttcatcc gatccgacac cgatctttca gatcaggcct
    14221 tccaatcgct tgccaatcat cgtcactctt tacaaatcta tatccaaaat cattccgatt
    14281 tcagaacgtc tcttttgccg gttgatgaag accatcttgc ccctgcaatt gtccgggata
    14341 tgatcgataa atctaaaata tgcggcgttg gtccgatggc gggcgtagca ggcgcgatat
    14401 ccgaatttgt cggacgtgac cttttaagct ctacaaaaaa catcattatt gaaaatggcg
    14461 gggatatttt tctgcaatct caggatacat tgaccgtatc aatttttgcc ggagaatctc
    14521 ctttaagcca taaagttcat ttagaaatca agcccgaaga aacgcctgtg ggaatttgca
    14581 catcatccgc ttcggtggga ccttcattaa gtttcggaag tgctgatgcc gtgtgcgttc
    14641 tttctccatc agccaccctg gctgattcgg cagcttctgc gataggcaac agggtaaaaa
    14701 acaactctga tattaagtcc gctttggatt tcggaatgaa tatccccggt atcaggggaa
    14761 ttgtcatcat ctgcaaaaaa gaaatgggta tcataggatc cgttcgactt ctttagataa
    14821 aacatcacaa ctcattataa ttctatagtc attccggcgt tcaaaaattt attttagaag
    14881 aagggttaaa gtgattcaat gggatcagag acttttttta atcgaccgga tggttgtata
    14941 tttccccata ccgttatgtt tacataacat atcttataag acataataat tttgattaaa
    15001 tagccgcata atccaagtct ctatatttat cgtgattgat ttagctatga ttattagatg
    15061 ttttattcgg tgtggctttt ataatccatc atcggcgagt tgctgtataa tttttatttg
    15121 acggtccgtc agtctacggg gaacatcaac atttattttt acgaactgat cgcccttcaa
    15181 tgctcccttt aatccgggca caccgaatcc tttcatccgg atcttcgtat tattttgagt
    15241 accgggagcg atcttgatcc gcttggttgt tccgtctagg gtgggaacat cgatggtcgt
    15301 tccaagagcg gcttgagtaa atttaatcgt tttttcgata tggagatcgt tgccgtcgcg
    15361 ggcaaaaatt ggatgcggca gaacattaat attcaaatat aagtcaccgc tgggaccacc
    15421 attaaagccg gacagacctt tgccgggcag ccttaatttt ttaccggcgc tgattcctgc
    15481 tggaattttg acattaatat cttctatgcg attttccagt tgaaaagaaa tctttttttc
    15541 cgcccctaaa acggattctt ctaacgtaat tgataagtta tattcggcat cctgtccttt
    15601 ttgcggcata tttgcgtaac gtgctcggtt tccgcccatt gcaccgccga acaggccggc
    15661 aaaaggatcg tcatattctc cttcgtaccc cccccttttt ctgtttgttc tgaaggttgc
    15721 cctgccccct ttgatacctc caaagccgaa gcttctgagt atttcatcaa gatcgaaatt
    15781 acggaaaata tcctcctgcg aataccgctg tcggaattga tcggccgaac cgaattgatc
    15841 gtattgattg cgtttttctg gatcgctcag aaccgcataa gcttcactga tttttttgaa
    15901 tttttcctcc gccgttttgt tattggggtt cttgtccgga tgccatttaa gagccagttt
    15961 tctataggct ttcttaatat catcagctga tgcctttttt tccacaccca ggacctgata
    16021 ataatcttcc gccatatgtt ttacctctta ttatttaaat atatttgatg aaagcatcag
    16081 gttttatatt aagcatgaat tcccatttga aaagcttcca gattcacttt tattaacttt
    16141 tccggaaacg actttttaat gacacgttcc caaatggatt tgtcgatatt catgtggttg
    16201 gataaggcgc ccagaaggat aacattggcc gtttttatgt taccagcctt ctgggccagt
    16261 tttgacgcgt caaaactgat gaccgttttg taattatttt gcaaaaattc aacggcatca
    16321 tcaggataag ggctctcacc catgttgacg gcaggaggat agacttcctc cgtattcacg
    16381 acaatggcgg cgtcttttct gagaaacgaa agatatcgca gagattccat tttctcaaaa
    16441 gatacaagtg tctgtatgct tccgggctcc gcaagcggag aatagacctt cttcccgtac
    16501 cggacgtggc tcgttacgca tcctccgcgt tgggccatgc cgtgaacctc gctttttttt
    16561 acgtcaaaac cggcttccat gatgacttca cagataatgt cgcttgcccg caggataccc
    16621 tgcccgccga ctccggcgaa taatatgttt tttacttcat tttcagacat ttagataatt
    16681 cctttaaatt aacttatagc gttgaattta caaacctgtg cgcataatcc gcaaccgttg
    16741 cataatgttg catcaatata agaaacgccc tttcgcttgg agcctttttt agttgcccct
    16801 tttttccagg aaatcggcgg acaattcagc ccgatgcaga cccggcatcc ttcacatttg
    16861 tccgggtcga ctttcaaggc cggtttgggt tttatatggc ttctcttgaa tagcgcgcag
    16921 ggtcgtcttg caataatcac agaaggaccc ggttttgtca gctcttcttt tatggcggac
    16981 gttgttgctt tctgatcgat gggatcgaca accctgacat gttcaatgcc cagaacggaa
    17041 accagatttt ccagcttcac ttgctttgtc tctatcccca tcagcgtata gccggtcccc
    17101 ggatgctcct gcataccggt catggccgtc gtgctattgt ccaggataat gatcaacgcg
    17161 tcactttgat tgtaagccat atccaaaagc ggggttattc ccgaatgcag aaacgtggaa
    17221 tcgccgatga cgcccacaac cttgcccttc cctttttcct tgagcgcctt gctcatgccg
    17281 tgagccatac cgatactggc gcccatgcag atgcaggaat gcagcccctc caaaggtttc
    17341 atgtaagaca gcgtgtagca gccgatatcc ccatgaacat aaaccttatg tttttttaag
    17401 gcataaaaca atccgcgatg cggacaaccc ggacaaagat tcggcggtcg cgccgccaaa
    17461 ggcaccttgt aatcggacac ttttttgatg gattgctttt caatcgcatt tcggatgact
    17521 cccggatcaa attcgctggt ataaggaaaa atttccttgc cggtgactgc tatgcccatg
    17581 gctttgatct gttcttccag aaaaggatcg agctcttcga taacgtatag ttttttaact
    17641 ttagaagcaa aatcacgaat cattttttcc ggaagcgggt aaaccatgcc cagcttcaga
    17701 taagaatatc ccggaaaaac ttccttggcg taattgtaag gcattccggc ggtaataatg
    17761 cctgcttccg cattggtaat ttcgacctta ttttccggaa acgtttcagc aaactccttg
    17821 agtgcctgct ggcgcttttc cacctcggct ctccggattc tggcacagtg cggaaccatc
    17881 acatattttt ccgcgttata gacaaatccc gtcttgtctt cgtatacagc cggttcttcc
    17941 agagtgacaa cggattttga gtgagatacc cgcgtggtgg ttcgaaagaa aaccggcgta
    18001 tcaaaccgcg cgctgatttc aaaagccatt ttaatgtagt gtttcgcttc agcggcatcg
    18061 gccggctcca gcatcgggat tttggcgaaa cgggcgtaat tgcggttatc ctgttcattc
    18121 tgggagctgt gcaatgaagg atcatcggca gtaacaatca ccagggctcc gttcgttccg
    18181 gtataactga cggtaaaaac aggatcggcg gcgacgttga cgccaacatg cttcatgacc
    18241 gccattgcct tttctccggc gagggccgcg ccgatggcaa cctcaacggc aaccttttca
    18301 ttggtcgacc actcggcata aaccccgtca tatttacaga aattttccag aatttccgta
    18361 ctgggcgttc ccggataagc tgttgcaaac cttatgcctg cctcgtatgc gccgcgcgca
    18421 atggcttcat tacctgacat caatactttc aataatcaaa cctccaaatc aactgagaga
    18481 agaaatttta tatttgatga agacggcttt tgaggaatcc gctgttttct ccagggcttt
    18541 cttgtaattt tccagcgcat tctttctatc attcatcagt tcataaattc gggcgacatt
    18601 gctgaacccc actgattcaa atccgatgtt gttgcccttt tgcgcttttt gaaaatattc
    18661 gagcgcggat ttgtaatctt ttctggcttc gtaacaatat ccgattgacg acagagcgag
    18721 aaatttaatt ccggcgtggt cggaaatccg ggaagaaacg aaatttttgt ggtgggcgat
    18781 ggccttgtca tattcaccga ggctgtaata aatattcccc agccggtaat gcgccatcct
    18841 tgcgctccag ctgtgcggat atttctcgat cagttcctgg aaaatccgga tattgttccc
    18901 ggtcatatcc tgtgtttcac ttccggaagc gctcgattgc gtccgggcat acatcgctct
    18961 ggcggagtcc tgatagttgc tccagtacat atagatacca aaggcaatca ttgatgccag
    19021 aatgacagcc gtcacaatgg agtaaaaacg ctttttgttt tcagaaatgt aatccttgac
    19081 cagttccacg gtgttttgaa aagcatccgg tccttccaaa tcctgttttg ttaatttctc
    19141 agccatatgc ctcctctgtg ttgatattat attttttgac caattccaaa agcaattgag
    19201 gtattctgcg aagcttgccc tcgtgattgg tgcaagcgtg aaccgtatag ccttccgcca
    19261 gaagatgttt ttgatcttca tcccatatct tggaattgaa accaaccgtc gctttccgga
    19321 tataggcaaa ttctgtttcc accgccaaaa gctgatcgta tgtggcaaac ttcaggtatt
    19381 cgcagccgac cttaatcaac ggaaggttca aattcaactt tgtcaattcg ctgtaaggaa
    19441 cacccagctg ccgcatcagt tcgtttctgc cgagttcaaa ccacttgatg tagttcgtgt
    19501 gatagaccac ccccatggca tccgtatcgg caaaaagcac cctgattttt gttacattgt
    19561 taaccgacaa cgctcttcca gtccttgata aatttttcca tcagcaaatg ccccggagaa
    19621 atcagaagat tgccggcttt ggcgtttatc tcattaaagc tcattcccgg ggccctgttc
    19681 tctttttttt ccgaagacaa taccgcaaaa gggctcggat cgctgacatg ggtcctgaga
    19741 tccaacggtg tcggatgatc gcttaaaaca ataatcctat agtggccgaa tgcgcctatt
    19801 ttcgtcaaaa taggcccgac gattttttcg tcaaaaagtt caatcgcacg gattttgcct
    19861 tcggcattgc cttcgtgtcc catttcatca ggggcttcaa gatggacaaa aacaaagtcc
    19921 atgaaattca atacatccag agccattttt gctttacctt cgtaattggt atcaatataa
    19981 ccggttgccc cctgcaccgg acaaactttc aacccggcga tcgttccaat gcctttaagc
    20041 agatcaacgg cggaaatcat gcctcccgat agggaatact tctgagtcag agggacaatc
    20101 cgcggcttct tcccctgccc ccacagccag atggcattag cctctttttt gcccgcctgg
    20161 cgtcttttcg cattgaccgg atgatttttt aaaaggtcgg ccgcccgcct gataatggca
    20221 ttgacatcgg cggcagcatt tccgcgcggc agatattcag ccacgggctt tcccgtaata
    20281 tcatgcggcg gtgttgtgtc cggattggcc ggggcgttgc gccagaccag cagatgacga
    20341 taccccacgc cgggaaagaa ctccagatga tcggaaccga tgtgccgatg gatatcgcga
    20401 atgattattt ccgcttcttc gctggagatg tgcccggcgg taaaatcatc cataaacgct
    20461 tccggaccat cctgatcgcc gatggtaacc agattgcagc gatacgcgac atcatctgga
    20521 ccgagatcaa ttcccatgct tgccgcttcc agcggtccgc ggccggtata attttcccgg
    20581 ggatcatacc ccagaacgct caatgtggcc acatcacttc cgggatgaat tccttcgggc
    20641 accgtatcga caaggcccag tgttccttct gcggcaatct gatccatgaa cggagtgaat
    20701 gcgcattcca gcggtgtttt tcctcccagc ttatcggtcg gataatccgc cataccatca
    20761 cccagcaata ttacgtactt catatcacct ttctgagaaa tctctttttt gaataattta
    20821 atttaaatcg tcctcaattc tgatcaggat ggtttcaccc tggaggatgt ccagtttgct
    20881 tattctcttg agtgctttct gcacatcact ttccttggct ttgtacgttg tcatcacaat
    20941 gggcaccgct ccccttttca ctctggcttt ctgaatgcag gcttcaatgc tgatattgta
    21001 tttcccgaga atacccgata tcctggaaag cacgccggga cggtcaaccg cggtaaaacg
    21061 gaaataatat tttgtctcca catgatccat gggaatcagc cggattggca tcatggccga
    21121 ctcgttgatc gaccgcaagg gccctcggcc gttttttccg ctcagaacat ctcttgcact
    21181 gtccataaca tcgctaaaaa ccgcgctggc ggtgggcatc atgcccgctc cctgaccgaa
    21241 aagaaagacg gggcctgagg catcgcccat gatatgaaag gcattgtaat tctgattcac
    21301 atttgccaga agacattttg acggaatcat cgtcggatga attctcgctt ctaccgcatt
    21361 gtttctgagc attgcgatgg ccagcaattt gatccggtag cccagttcct tcgcaaacga
    21421 aatatcttca cggctgattt tggtgatacc ttccagatat atatccttga gattcacttt
    21481 cctgccatag gccagtgaga gaataatggc cattttgtgc gcggtatcga tcccttcgat
    21541 atcaaaggtc ggatcggctt ctgcaaagcc cagcttctgc gcctctttca aaaccatgtc
    21601 gaacggcttg tcgtcatccg tcatcttgct caggatgtag ttgcaggtgc cgttcatgat
    21661 gcccatgacg gaaatgattc tgttggcaac caggctttcc ttcaaggtct tgatgattgg
    21721 aatggttccg ccgacactcg cttcaaaacc gatattgacc gcatttttct gagccgccgg
    21781 gaatatttca ttgccgtggg tcgccagaag cgctttgttg gcggtaacca catgcttgcc
    21841 gttggcaaca gcttcgagaa caaacgtgcg cgccggctcg tacccgccca tcagttcaat
    21901 gataatcgga atgtctttat cgttgatgat ctcccgcgca tcccgcgtca agagcccctt
    21961 atggaccctc acttcacggg gtgtcgtgat atcaatatcg gcaattttct ttataacaag
    22021 cctggcgccc agtttttttg taatcagctc ttcattttgc cgcaacagtt tgaccacgcc
    22081 cgttccgatg gttccgaatc cgataagacc gatagggatt tctttcatta tatgcacctg
    22141 aaattatttt ataattttat ataattcccg ttaattgtta atctttttcc gttaatttcc
    22201 tataacaaat ggatcggctt tgtcaatgaa atatccatca tgaatgctta aaataattaa
    22261 tgaattaccc agacatgaag taaaccgatt aaaaaccgga tattttgcaa aagccccatt
    22321 acttgaccgt gatcggttta ttttcaacac cggcatagaa aacaataatc tcggccgctt
    22381 ccttgccttc atttttcccg taatgtttct tgttgaccag ttccacaatg gaatctccgg
    22441 ctctgagatg caaagttttg ttatcgtccg ccacaaccgt caattctccc ttgagcaaaa
    22501 cgcccgcgtt aatcaccgga tgatgatgca tttccagttt tgaaccggca ggaattctga
    22561 tgcgcagaat tgtaacctcc ggctgaccct ggaggtattg cggcagcgtt tcgccatccc
    22621 agctcctgac tgttttggcc agttgtaccg tttcaatgct atgtaaaccc gccgaacacc
    22681 cggcaaacaa aaaaaataaa accgacggta ttacccagat gagctttttc aatgtttcct
    22741 cctctcgttg gattaaaaat catatagggg gattattcta attaccgcgc acatggcggg
    22801 cataccatcg ccgtcccagc cagcgttgat ggtgccaggc aaaaaacggc aagattcccc
    22861 tttttctcaa ccagtaacct tggggaatct ctcttgagtc ctgttttggc atcattagaa
    22921 tttccgcact gacaacccct tcgccttctt ccggtttacg gtgcgcaagt atccgccctt
    22981 ccgcatcaag aataacggca ttgccttcgt aaaaccctgt atagctcatg ggcagtcccg
    23041 gcatggcaca ttcaatttcc ccgcaatgtg cggcatgaat caccggtacg cctaaaatac
    23101 gcgcgttatc ccggagcacc tccaacgcat tgcgtttgtt gtccggttcc cagagtttct
    23161 gcaaaaaaag cggaaaatta gtcggtatgc tccaccagca tgatccgccg atgaccacat
    23221 ccaccttgtt ttgcagccgg cgggccgtca tcgttcgcat aaattcccag cagaccgccg
    23281 cgccgactct gacattttcg tataagccga gcacgccgat atccccggga tcgccgcctt
    23341 cataaaaata attttcccac atggttggaa tatctttatc atgctgaccg acgagccggc
    23401 cttgcgcata acattgatac cggttgcgga cttgcccgtc gggcaatcgg cataaaaatg
    23461 atcccccgat caaaacgcgg tatttttttg aaaagccgca aagaaatgtt gccgcctcgc
    23521 cgtccgcaga ttgaatagcc cgggcaatat tcggattcca gctgacgccc gtattgaaaa
    23581 attcaggcaa ggcaatccat tgtgctcctt cgtttaccgc ctgcagggaa aggcgctcac
    23641 agcgccttat gttttcctct gattcggcca cggcagtggc cagttgaatg gctgcaactt
    23701 taattgtcat gggtttattc cttcggctct ttgattgatt ttaattgcgc ataaacgtca
    23761 tcatgtaacg gcgtaggcac gccataatcc cgaccggccc cgcaaagata agccgtcagt
    23821 gcgtcaattt ccgtctgccg gcctttttcg aaatccagct gcatggatgt tctggaatga
    23881 tatccgaacc cggcaatcat cgccatcgct ttatccactt catgctcctg tataacgaca
    23941 tgatgtgcgg cggcgacggc aaccacctcc atcatcatcg cccttgcttt ttgccgtaat
    24001 tcaggatttt cccatatctg cccgtatgtt ttgccggtgg ccgatgtcag agaccccagc
    24061 ggacacatta acaaatattt tatccagagc acgccggaaa tgtcgtccgt taataccgcg
    24121 ttgatctttg cctgaagtaa aatatcaagg atatatcgat aatcgtctgc cggatcatga
    24181 tcggttccga acgtcagctt gcacgcgcct ccttcctgct taatgattcc cggtccgtca
    24241 ctatgggaaa tgatataaac gctgccgcct aagactatcg catgcggcag gacacggcgc
    24301 agccgttgcg agctgttaac accgttgagc aacggaatca ccacggtttg tctgccgatg
    24361 caggagatga acgcttcagc cgaattttcc aatgaataac ttttggtgca aaagaacacc
    24421 acatcaaaca ttcccgcctc atcgggattg tttgtggcta ttctcggccg gacaacacaa
    24481 tcgccttcac gcgtaaaaag ttgcaggccg tttttttgaa tcgcttttaa atgttccccg
    24541 cgggcgataa atatgatttc atattcgtcc gatttttcgt actcacgcgc cagtttcccg
    24601 ccgaaatatc cgcctacccc gccgatacca atgatgccta ttctcacgcc catcatgcag
    24661 ctccatccgt ttgaaacgcc ttttcattaa aattatcctg cattgttatt gaagtcaagc
    24721 ttcatcatat tgatcgtcag cttgctttta tgatcttcga tgtggtaggt tactcttcac
    24781 aaaaaaaccg gatatcaacg caataaaaag caaactgcaa ataaggagag cttcatgagg
    24841 attcaatctt ttgtcgttca tttgcgtttt aaatcggcaa ttcttttcgc atcgatctta
    24901 tgcatagtca tcagcggctg cacgcctttg ctgaacgtct cctacaaaaa atccctgcct
    24961 cccatggaag gagaacaggt tcttcccggt ttgtccgata acgtcactgt caaacgcgac
    25021 aatctgggca ttccttacat tgaagcgcag agcatggacg atcttatctt tgccatggga
    25081 tatatcagcg cttgtgaccg ctttacacag atggaaggat tcaggcttgt gagccaggga
    25141 aggctctccg aacttctggg cagggccacg ctggaaatgg atatttatct gcgggcgctg
    25201 aatctcaatc aggtcgccca gaccctttac ctgtcggcat cctccgacct gagacgactg
    25261 ttaaatatct acagcgacgg cgtaaacgcg tatctcgcca acgtccctct gcccacgact
    25321 ctgaagttgg ccggacacca acccgatccc tggagaccca tcgactccat gtatgtattt
    25381 cttgttctaa cgctgggcct gagtcaaaat ctccaggaag aaatcaacct gctcaacgtg
    25441 gcgcaggttg tcgcaccgga aaagttagcc tggctttttc ccatttaccc cgatgaaccc
    25501 ctgccctttg aagaaatgaa aaaactgaaa ggaatcaatc tttcagaggc tgctcaaaat
    25561 attgaacaac tgggcgtcat agctaatttg accaacacac tgcttcttcc tgccgcggcg
    25621 gcttccaata actgggcggt ctccggaaaa ctgaccaaat cagggaaacc catcgtggcc
    25681 aatgatacgc atctgccgtt gaccatgccc tccatctggc acatgatgca tctgaaatgt
    25741 ccggagattg acggagccgg tattggcatc gccggtgttc ccggcattgt tgccggatac
    25801 aacaggcaca tcggtatcgg catgacgatg gtcatggcgg acaatcagga tatctttctg
    25861 gaaaagctgc aacagaaacc ggacggtctc tattttcttt ataagaataa atggttgaag
    25921 gcaaacagca gggaggaaag cttccggatt aaaggacaaa aaaaggctgt tacccgaacc
    25981 ttttatgaaa ctctccacgg cgttttgctc aatgatattt tgaccgataa gccccgtcat
    26041 gaactggttc ccatgccgct taatccttca ttgggaatcg cgtatagttg ggcaggcaat
    26101 gaaacggaca ggtccatgga cgtttttttc aacatcatgc tcgcaaaatc ggtcgatgag
    26161 attcttaaat acacttctca ggaaacgtcc gtcattccac tgaaccttgt catggcggat
    26221 cgtaaaaata tcgcctggca ggttacgggc cgtttcccgt tgcggaaaaa aggtcgcggt
    26281 ctctgtccct ctcccggttg gacgggtgaa tacgattgga acggatttct ttcaccgtca
    26341 aaacatccgt ctttgaaaaa ccccgcgaag ggctatgtgg gcaccgctaa tcatcggaca
    26401 gttcctgctg attttcctta tattctgtcc tcgtcctggt actatcccga cagaggcgaa
    26461 cgtatcgaac agctgctgaa aaatgcaaaa aattacaccc tggatgatgc aaaagctatg
    26521 cagcttgata cctattccag gtttgtcaac gtaatcaaaa cggcgttgct ggaacaaaaa
    26581 acgctggaca ccatgagttc tgtctggcaa aactctgaaa aatcaacggc ggctcatcaa
    26641 gcgatggcac tgctcagaga ttttgacgga aatatgcgcc tcgattccgc cggggcggct
    26701 ttctgcggcg cttttctgtt ttcgctgagc gacaatcttt tcgctgacga actgggaggg
    26761 aaaaatacac gtgcctatca atcactgctg cataccttcc tgctggccta ttccgcactg
    26821 cacgaccatt tgacggacag agggaaaaga agccccctct gggatgacgt aaccacacct
    26881 cagaaagaag aacgcgacct gattctggcc aaaacggttc tggatgccat cggcattctg
    26941 gaaaagcgct gcggaaaaaa tcccgacaag tggcaatggg gaaaactgca tacctatcat
    27001 tggaggactg atgccacatt gtttgccgat tatatggatt tcatcaacaa gaccggcgtc
    27061 aaattcctct ccggttatgt agacagaggt ccctatccgg cgcccggcga ccacaccacg
    27121 ctgaacgttt cagcttatca tcccgggaaa gatttcggaa cctggatgat tccggccatg
    27181 cggatcattg tagatttcgg aagcgatgaa cctttcgtcg gcattaacag cacagggcag
    27241 ttggataatc cgtcaagccc gcactacgat gacggcatca cggcattccg gttaggacag
    27301 tatcagaatt ttccgtttaa ggaagaaaat ataaaaacac agtacacgaa aatactgaca
    27361 ttggtgccca agaaatgatt taaaaaaaaa gaatatgctt tgattgcttt gagccgccag
    27421 acacattatt catgatatac caacttggct aaagcgctta acagccccct aagttcagtc
    27481 gagtcttttg gactcgacta tttatctgtg tttgaataca gccaagtcaa aaagacttgg
    27541 ctgaacaaag tccacctgaa cccgcaagct tatgattccc atccggggaa tagagaaaga
    27601 atttacctgc tgacatggat ttttcataaa tcaacacaga accgtctttg ctcaaaagcg
    27661 gccgcttagg tcgggtcttc ggcgggaatt tataaggatt gggaatcagc tctttccctt
    27721 tcatcacaac aatgtaatga atccccttgc cctgtttctt tgcttattac cccgttctgg
    27781 ccaaatccag tttcccctgc catttccaga ttaagacctc cttcagaatt tgatgttcaa
    27841 ctctgtccag aaacggatcg cgcgcagcca aagcgaaagc gtcctccttg gcctcgctca
    27901 aaatacgggc atcgcgaata atactggcaa tgcggaaatc cggcagcccg gattgtctcg
    27961 ttcccagaaa atcgccggga ccgcggatag caagatcttc ttccgcaatg gtaaatccgt
    28021 ccgttgttgt ttccatcact tttaatcttt tgcgcgcatc tgctgaacat ttataattgg
    28081 ccaacagaat gcagctggag agaatatcgc gccgccctac cctgccgcgt aactgatgca
    28141 gttgcgaaag tccgaagcgt tcggcatgct cgataaccat taatgaggct ctcggaacat
    28201 cgatgcccac ttcgatgacc gttgtcgaaa cgagaatatg aattttgttc tccataaatt
    28261 cccgcataat ttcatctttt tccttttcct tcattttccc gtggattaat cccacgcggt
    28321 aatccggaaa aacgtcctgc tgcaaatgcc tggacatatt ggtggcgtct ttcaaatcca
    28381 gcgtttccga ctcttccacc agcggataaa caatgaatgc ctgatgccct ttcgccagtt
    28441 ccgcgcggat ggcctgataa actttgtccc gtttgctttc cggcaaaaca acagtgtgca
    28501 cgggcttttt ccccggaggc atttcattga tcacagagac atccagatcg ccgtatacgg
    28561 tcatcgccag ggttctcgga atcggggttg ccgtcatgac cagcacgtca gcgttgatgc
    28621 ccttggatcg caatgtcgct ctctgaataa cgccgaaacg gtgttgctcg tcaatgacaa
    28681 ccagccccag tttatgaaaa tcaacacctt cctgaatcaa agcgtgagtg ccgataatga
    28741 tgcccgcctc gccgttttga atccggcgca gggtttcgcc ccgtgatgca ttggccatgc
    28801 tgccggtaag caaaaccgtt ttcaatccca atggctcagc ccacaaacgg atattttcaa
    28861 aatgctgttt ggccagaatt tccgtcggcg ccattatggc cgcctgataa gaattctcac
    28921 aagcggtaat catggctgcc attgaaacaa tggtcttgcc gcttccaacg tctccctgaa
    28981 ggaggcggtt catcgccgtg tctttcgaca tatcgttgag gatttcttca atgactcttt
    29041 tttgagcggc ggttaaatcg aactttaaac tggaataaaa tttatccagc attgtgccat
    29101 cggttttgaa tgaaaggcct tgatccagaa ttcttcccga ttttttgaca gccatgccca
    29161 actgaaaaaa gaaaaattca tcgtaaatca aacgccggtg agcctcggaa cggctggaga
    29221 tgaattgatc aatgtcctcc tgactgccgg gaaaatgaac agaccacaac gcttcatgga
    29281 cattcagcaa atgtcgcttt tcacatatac tgtccggaat cggagaggca acatagcggg
    29341 aataattcat gagtgcggca ttcatgacct tgcgataata tttctgataa aggccctccg
    29401 tttcggaata cacgggcacg attcttttga aattcagaag attttcttca tccttctcgt
    29461 caagaatttc ataatccgga tgaatcatct gttttccgac gtaatccggt cttacgtcgc
    29521 ccgtaaaaat aaccctggtt ccttttttga aaacacccag gagatacgcc atctgccctt
    29581 taaaccactt cgcggtcaga ttggccgtgc tgtcgctgat caccgtttcc aggatttttt
    29641 ttcttccgta atagcgatat tgacagctga tcacgttgcc cacagccgat tggacttttt
    29701 cggcttcgag acggttgatt tttctgattt ccctgcggtc ttcataagtc cgcggcagaa
    29761 agaaaagcag atcttccacg gtggcgattt ttttattggc gaagcgggac gccattttcg
    29821 ggcccacacc tttcaaatac tgaaccggca gattcagttt ttccagggct tcttttaaat
    29881 ccgatatctt ctcaatggtt ttttgatcgg cattccgttc gggaacggat atgttcaaaa
    29941 aatggatgat ggttttgagt ttttcgacga ttttgacggc gttttcaatt tttctttttt
    30001 ttaattccag cttttgccgg tcgtaatcgg aaaagatttc ccgtaatgct ccgatctcat
    30061 gattaatttc ctcacggccg aacgatgcgg caaaagaatc aagtttcgac aaaagcgtta
    30121 tgagcgttct gcccagatct ttgatgttgg aaagattttt gtaattatcg cggaaagcaa
    30181 agttcagggg ttgatcaatt ttttggagaa tttcgtttaa ggcatccatt taaatctcca
    30241 cgccgcaaat ctgtcaaacc tgaatcattt ctgatgaatc cggatatttt tgttaacaaa
    30301 tatattttat ttgcgcaagg aatgattgac ttaagcgttg aattacgtta aaaactcacc
    30361 ccgaaagaac ttccatatga aatttaagat cactcagagg agctatgaca gacaatataa
    30421 cctataaaga tgccggcgtg gatatcgaca tcgccaacga atttgtggaa cgaattaaaa
    30481 catccgtcaa aacaacggcg cggaaggagg ttgtctccgg tatcggcgga ttcggcggcc
    30541 ttttccgtct aaattccgtt gacctgaaaa atccggtgct ggtatcttcc accgacggtg
    30601 tgggcaccaa gctgcgtatc gcccacctgc tcgacaaaca cgataccatc ggcatcgacc
    30661 tcgtcgcgat gtcggtaaat gacgtggttg tgcagggagc cgagccgctt ttctttctcg
    30721 attatctcgc taccggaaaa atcgagctcg aaaaaagcgt cagcattatc gagggaatta
    30781 cggaaggatg ccgacaggca ggctgtaccc ttctgggcgg cgaaaccgcg gagatgcccg
    30841 gtttttatgc tcccggagaa tacgatctgg ctggattctg tgtcggagtc gttgaatcgg
    30901 acaaattaat caccggatca accatcagtg tcaacgaccg tattatcggg ctggcgtcca
    30961 gcggactgca cagcaacgga ttttcactgg cgcggaaggt tcttctggaa aaaggaaagc
    31021 taaacgtcaa cgataagctt cccggactgg aaaaaaccgt cggtctggaa atgctcgaac
    31081 cgacccgcat ttatgttaaa gccctgctga atctctttaa aaaattcacc ctcaaaggcc
    31141 tggtccatgt caccggaggc ggattctacg aaaacattcc gagaattatc ccccaggcct
    31201 gccgtgccgt aatcggcaag ggcagttggc ctgtcccgcc gattttcaac atcattcgtg
    31261 aaatcggcaa cattgatgaa aaagaaatgt tccgcgtatt caacatgggc atcggtatga
    31321 tgattatcgt ttccgacaaa gactgtccgg cggtgctcga atatctggaa attctcggtg
    31381 agaaagcgta cccgctcggc ctgattgaaa tcagggaaga taaaaagccg caggtcatca
    31441 tttctgaaag ctagggaaag caaatggcgg atgcattgaa actgggaatt ctgatttcag
    31501 gaaacgggtc caacctgcaa tccattattg atcacatcga gcaggggtct cttcgggcgg
    31561 tcatccaaat tgtcatcagc aataaccctg atgcttacgg catcatccgg gcaaaaaaac
    31621 acggcctccc tttttgtgtg ctgaaaaaca gcgattttaa aaataaagag gaatttgatt
    31681 ctgaacttat caacacgttg ataaaacatg acgtcgagct ggtaacgctg gccggtttta
    31741 tgcgtatcat ctcgcctcga tttcttcagg cttttcccgg gaggatcatg aatatccatc
    31801 cggcccttct tccttctttt cccggtctgc acggacagcg gcaggccgtg acgtacggcg
    31861 tgaaagtttc cggctgtacg gttcatttcg tggatgaggg cgtcgatacc ggcccgatca
    31921 ttatacaacg agccgtgccg gtgcgcgatg atgacaccga ggacaccctg gcggctcgaa
    31981 ttttaaagga agaacataaa atctatccgc aggcgattca gctttttgcc gacggcaaac
    32041 ttgaaatccg gggaagaaaa gttttcattc acccatagcc gtttttctcc ggtctaatgt
    32101 cttttttctg atgatccggc aatgacgttt aaggacagaa tccatgtatg atctgattgt
    32161 cataggcgat gatctttctt cccatgtagc cgcagcgttc tgcgccatca acggtttgaa
    32221 gacggccctc ttctccgaaa aaggaaccgc cggctcatct gttgccggag atctcacgtt
    32281 tgacggcacg atcacgccgg tgtccgggtt tgccgaaaac cagatcgcct atctgtcatt
    32341 aaaaaatctg ggaatttatc cggaggtgcg cctgctgaat ccggcctatc agattattct
    32401 cccatcgcaa aggatagact ttttccatga caaagaagaa cttctgaaag agctcgccag
    32461 agagtttcct catctcgagg gcgatcttcg attattctac gatgtagcag agaaaaattc
    32521 aaacgcagcc ggacaatgga tttcagaaca tcccggcatt caaccgaaaa aaatgaagga
    32581 ttttttcgat tatctcaaac tgattccccg tttcgtcgaa agcatgttca acaccatccg
    32641 tttgaaacgt ctgatgtcgc aaaacccatc cttccggaaa gttattcagg ctcagcatgc
    32701 ccttctttct ttcaaaacgg atccgttaaa ttcttttttt tcatcatggc aatcctgcac
    32761 gccgttgcgc ggcaccttct cctttgctca gggcaaacag gacctggtcg attcgctgat
    32821 ccgtaaaata aaagcgtccg acggtcttta tttatccgga tgcgattgcc tgtcgatcca
    32881 taaagacaag gtcattgaac tgaattatac ggacgataac ggcaacaccg aaaaaatcga
    32941 aagccgccat ttaatcgtca gcacaaaatg gcaaaacatc ggccaattgt tcgaccacaa
    33001 gaaaactttc agttttggcg attttcttcg gccggcaaaa acggcgtatt tccccttttc
    33061 catttttctg ggcgtcaatc cggcagggct gcccgaaaag ctggccagac attcggcaat
    33121 tatctgcaat ccggccaggg agctttatgc ccacaacaac ctgatcattc tggaatcgtc
    33181 ggcaaaagaa gagaggatga aggatcccgc tgcaaaaatt cttctgagcg ccaccgtttt
    33241 cctccccgat catcaggaaa tctggtcaaa ggataacctg aagactattt ccgaaaacat
    33301 catgaatcaa cttgaccatt tcttcccgtt tttaaaagaa aacattttgt ttttcgatcc
    33361 ggacgaatcc atgaagctat cgcttaagct gcgaggcacc atcatcccgc gctatcagat
    33421 caaaaattct ttttttacgg gtttttccgc taaaggcagt aaaacccggt acgccaacat
    33481 ttatcttacc ggagcgtcct tactggcgga tgccggtttt gaagcggaaa ttctttccgg
    33541 actcaatacc gcgaatcttc tgatttcaca aaggaagtaa tttatgaaag acgttttttc
    33601 catttccaat ctcggcaaaa gaatgattgt ctctcccctt caaacgggca agtttacccg
    33661 ggacggcaaa agagccctgt ttaacactca tctggacaac tatcgttcgc tgaccgataa
    33721 aaaaggacaa ccgctttccg tcgagttggc cggcccccgc gaaaagattt tttttaatcc
    33781 tgccgacacc agagcggcta tcgttacctg cggcggcctt tgcccgggta tcaacgacgt
    33841 gatccgctcg atcactacag cgctgtactt caattatcac gtcaagaaga ttgttggcgt
    33901 caaatacggg ctccagggat taaatcctca ttgcgggcat ccactgatct ctctgtcgcc
    33961 ggatgttgtg aaagacatca gcagcttcgg aggcagcatc ctgtccacat cgcgcggtcc
    34021 gcaggatgtg caaatcatcg ttgactatct tcacagatta aacatcaaca tcctgttctg
    34081 tatcggcggt gacggcacca tccgagcggc ggaaaaaatt acagcggaaa ttaccagacg
    34141 aaattttaaa attgccgtga tcggcattcc caaaaccata gacaatgatc tccatctgat
    34201 tcagaagtcg ttcggatttg ataccgcgat tgagaaagcg gccgatgcga ttaaaagcgc
    34261 ccacgtggaa gcccggggca tggtcaacgg catcgggctg atcaaaatta tgggacgcca
    34321 ctccggacac atcgcagccc acgcagcgct ggcacaaagc gatgccaatt ttgtcctcat
    34381 tcccgaggtg ccttttgatc ttgaaggcaa aaacggcttt ttgggttttt taaaaaaacg
    34441 catgatttcc agagggcacg ctgtcatcat tgtcgccgaa ggcgccggtc aggatttgct
    34501 cggcacaacg gaccgctccg cagaaaaaga tgcttcaggc aatgctcgtc tgctcgatat
    34561 cggatcgcat ctgaaaaacg tcatcgaaaa ttattttaaa aaaatcagca tggagatcaa
    34621 tctgaaatat attgagccaa gctatctgat tcgttcggtt ccggcaaacg cttcggatga
    34681 catttactgc aatatgctgg gacaatacgc cgtgcacgcg ggaatggccg gaaaaaccgg
    34741 aatcctggtc ggtttgatga aggatgagta tgttcatctt ccgttggccg cagtcgcctc
    34801 cggcagaaaa atcgatcccc gcggggatat ctggttaagg gttctggaga cgaccggcca
    34861 gccggcctcg atgaaaaatt aatcgaatat taataatatc ggatagttaa gaaaatattt
    34921 tcatcgtctt tcagccggat aatttgtttt ttattcacaa caaaggacga ttgacattta
    34981 tctttcatga ttggcgaaaa aatgcttgca aaatactgtc ttatggttta gtaacgacgc
    35041 acattttctg gaggattgta aaaatgtctc gtgtttgtga agtatgcggc aaaaagccgt
    35101 ctgtcggtaa tgccgtgagc catgcaaata ataaaaagaa ggcggtctgg tatcccaacc
    35161 tgcagaacct gcgttgtgta gatgaaaaaa ccggatcaat aaagagaatc aaggtttgca
    35221 cacgttgtat tcgctcagga tttgtcaaga aagctacctg ataaaagaaa ttatttgaga
    35281 ttcttttatt acatcggctg tttcattgaa aaacagctca tcatcgtcaa tcaaatccat
    35341 cgcctgctca aacaaacggt tcaattcatt attaagcaat agatcattgc agggaaggcc
    35401 gaatttatct tgataaagat gcattcggcc ttggatggtc atcatttgct ccatcgattc
    35461 ttgcgtgatg aacaacaggg cgcttctcat cacctctcga atctgattta cttccaggcc
    35521 gtagagccat cgcagggttt ctgtaaattc ttccatcctg ttttcaaccg tgtattttat
    35581 ggcgtcgacc atcacctcaa cagtcttaaa cgtgcggaga tgccttacga ttaccagatc
    35641 gtatatcatt tcgatgatca aatgggactg ataaacggcg tcgcgacacg aaatttcttt
    35701 gcccgcaatg ccatgtatat cctgtatgac tcggataaga ggattgcctt tccggtagga
    35761 atagccccgg gaatggatat cgaatgcatc gccgtcttcg gtacagatat atccgtaatg
    35821 cgaaagatca tcaatcagca gatgatagat gacataggcc gctttcggat actcaacagg
    35881 aagggtttgc agtcttctta atttatgcgt tctggcggag gttatatccg gatgaatcgc
    35941 cagcaaatcc ggggcgatat tgtatacaaa aatatccggt tccacatttt ttatctccgc
    36001 ggaacccaga attttctgca ggaaatatgt atgagtcagc atgaacatat atgataagca
    36061 gccatgaccg gttatcggat ccggtcatca actagttctt tcatgtgata ttttttactc
    36121 aattcatcga gcaagcaatt catctcgcgc agaaaatcgg gcatgatgga aatctgaatc
    36181 accgccacgg cggcatcgat ggtcgttacg gtagccaggc cctcgtaccc ctcgataata
    36241 aattgcactg cgacgatatt cttgcgatcc agctgaaaat acttgctcat catccgctca
    36301 tggtctcttg gcttcagtta tcgtttgacg tcatcagata aagactgtat gatcaataaa
    36361 aaatcaagga ttttttatgc gccttgtccg aaatacatcg attttgttat tattattcat
    36421 tgtttcctgc tcccccatcc atcccttcat caaaagcata tcggaaagcg atgttgcctc
    36481 tttcttgcag aacgctcccc gggcggagga acatccgaac gccggggccg atttactgta
    36541 ttcctacagt tatgtggaat tctttgaaga cggccgaagc atcagcagaa acctggatcg
    36601 cattaaaata ttcaacgaac gcggccgcag cttcgcctcg aaatcaattt cgtaccggga
    36661 aggctatcag aaagtgaaaa ttatttttgc caataccatc aaaccggacg gaaaaataat
    36721 cgctctggac agaaaagata tcatcgattc ttcggagtac gccggttatg aattctacac
    36781 cgacatcaaa gtgaagaagt tcaccatgcc cgcggttgag gacggctgca ttatagaaat
    36841 agcctatgaa attgaaaatt taaaacccgt tttatccatg gactactcgg aaacgttttt
    36901 ttgccagaac tttttcccca tcgaagaaga catcatggaa atcgtcctgc cggccggcac
    36961 aaaactgcaa tatgaaaatt tcagaaccga catcacaccc caaatcactt cgttgggaaa
    37021 caaaataaaa tatgttttca ttaacaagaa acagaaggaa attattcccg aatcccggat
    37081 gccctccctg ctcgatcgcg acacatttcc ccagatttct ttctggacgc taagcaactg
    37141 ggacatcatt tcccagtggt acatcaaact tttcaaagaa caaatgaagt ccagcgccga
    37201 actggaaaat ttcacccgcg agcttattgc cgatcaaaaa acggatgagg acaaaatcaa
    37261 cgccattttt aatttcgtat cccagaatat ccggtacatc gccgtgctcc tgggaccgca
    37321 tacccacaag ccccatcagg cgtttgaagt gttcgaaaag cgttacggcg actgcaaaga
    37381 caaaaccgtt ctgcttctga ccatgcttaa aattgccagc attcaaggct tgccggctct
    37441 tgttccggcc aacggaaaat atttcgatga aacgattcca tccatgaacg cctttaatca
    37501 tattattgcc gtcgtcccgc tgaaaggcaa atccttctgg ctggatgcca ccaatgaaac
    37561 gtcatcgtac gattcgcctc cgttcatgct gcccaccaga atctttctga tcaacgatga
    37621 cggctcatac cgtttcgtca cgaccccgcc gttaaacgac aagaaagatt actatcatct
    37681 ggcgattcgc tataagatcg atacggaagg caacgccgct attgattatg tttataattt
    37741 tttcggcaaa gcggccgaag ccatccggta ttccttcaaa tatgctcctc cggagcaacg
    37801 aaaaaaattc tttgagaacc gcggaattga aatcagagac cttgaactgg gaagcttttc
    37861 agacacccga acgccgttta caataaaact ttccggcatg attagaaatc tggcacaggt
    37921 tctcgacgag gaaaccatgg tcctttccaa tattatcccg ctggatgcat accgggacat
    37981 cacagccgcc agcatccggc ggtaccccat cagcctgacc caatccatgt atgcaatgga
    38041 gaataatacc tatcagtttc cgccgggctt taaaataaaa aaattgcctc aaaattatat
    38101 atttaaaaag cctttccgct accgaaaaga aaaatacagt tttacaggat ccgaatttcg
    38161 tgtttatctg gaaacgaaaa acgaagagca gaacattcct ctcggcaaaa tgaatgagtt
    38221 taaaaaatat gcttcggaac tgcaaaagca cgaatcagca gtaaaaaaca ttatttttta
    38281 taaaagatac caaaaccttg acacccggaa agacattgat attataaatc ataagtaacg
    38341 ggaaaagaaa cggcacaatg acaacacaaa gaaaattgcc atcgctgaaa tggaaaatcg
    38401 aggaagccgg caataaaaat gacggggctc tcttaagcaa agagtttggc attcatccga
    38461 ttattgcgca gattctcctc aacagaggta ttcgcgatgt cgataaggcc cgccattatc
    38521 ttcaaccgtc tttaaacgat ctacacagtc ctttcctgat gaaagatatg cgccagggtg
    38581 ttgcccgcct gctgaaagcc attcacaaca atgaaaagat catgatttac ggcgattacg
    38641 acgccgacgg gataacatcg gtcgtcattc tttataaatt cataagagaa ataacgcccg
    38701 tcctcgatta ttacattccc gacagaattc aggaaggcta cggcttaaaa gaatcggcaa
    38761 tcaacgagtt caaaaaaaac ggcgtcagcc tgattattac ggtcgattgc ggaatctccg
    38821 atgtggaaca gattgcctat gcccggtccc tgggcattga tacgattgtt ctggaccatc
    38881 atgaaatatc gggcggactg ccgcccgcag cagcggccat caaccccaat cgcgacgatt
    38941 gcccttaccc tttcaaaggt cttgccggcg tgggcatcgc gtttaacttt ctcatcgctc
    39001 tgcgcggcac tttgaacaag gaaggcttct ggaaagacaa ccattatccg aatctcagag
    39061 aatatctgga tatcgttacg ttgggaacga ttggcgacat cgctccattg acggacgaaa
    39121 accgaatctt caccaaaatc ggcctggagt tgatcaccga gggccgccgc ccgggcatca
    39181 aggcgctcaa ggaagtcagc ggggtggata atcaggtcat cgattctttc aaggcttcct
    39241 tttccctgat tccccggatc aacgccgccg gacgcatcgc ctccccctcc gacgccgtta
    39301 aattgcttct gaccgacgat ctgtcggaag cgcggcctct ggccgaaaag cttgattcct
    39361 acaatcgcca tcgccagttg atggagaaaa aaatattgag cgacattctg gaaatgatcg
    39421 gcaaaaaccg ttccctggaa aaaatgaatt ccctcgtttt cgcctccgat aaatggcatc
    39481 cgggggtgat tggaatcgtg gcgtcgcgtc tggttgaact ttttaatcgc ccggcttttg
    39541 tcatcagtat catcaacggc gtgggtaagg gttcggggcg cagcatttcc ggtttcaaca
    39601 tctacaaagg cattcagcaa tgcgaacact tactgctttc attcggcggc cactaccatg
    39661 ccgcgggtat atccatcgaa gaagccaata tcgaaaagtt ttccgctgtc ctcgacgaac
    39721 tgaccgggga cattgccgaa ttttctgaaa tcgcttctca aataacgatt gatacagaat
    39781 gtggacttga ggatatcgac ccggctttac tggcccagat ggaggctctg gctcccttcg
    39841 gcagcaggaa tcctgaaccg ctgcttctcg cccacaatgt taaagcatca tctcttgttg
    39901 tcgtcggaaa caatcatcta aaaatgagca tcaacagcaa tggaacctcc cgcgattcga
    39961 tctggttcaa catggggaaa catctctccg ctctggacgg agccagtctc gatattgtct
    40021 ttacgccgca gatcaacaac tggaacggtt cggcggacat ccagttaaaa atcaaagatg
    40081 tcaatattgt ttcatgacct ttaacaagaa tcgtctgatg gttgcatgat gaacgtggct
    40141 accaaagcag cattatttaa cgcgctggtg tttcccggat ggggagagat ttatctgaaa
    40201 aaatacaggc gaggccttct gatcatcacc ggcatggccg ccggaattct atccattcta
    40261 cttttagtcg ttcaggaaac cctggccgtt ttgaaagtta caccggtcga caaatcaacg
    40321 ttatcgctcg gcagactttt ccatttatcc gttaaggtga tgcattcact gaatccgtcc
    40381 tgcattttga tcattttatt ttttatgatt tttctctggc tcctctcgat tatcgacgcc
    40441 tacagactgg gcaaagaaac catgttggaa attagtactg acgctgatca acaatcagct
    40501 tctcctgagg cttgatttca ccggccctaa tatattccag agtattaatt cccaccgtgc
    40561 agattt
//
LOCUS       DAOSME010000016        39379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1360_length_39379_cov_22.900137, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000016 DAOSME010000000
VERSION     DAOSME010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 39379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39379
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1360_length_39379_cov_22.900137"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1032..1119)
                     /locus_tag="PLG98_03880"
     tRNA            complement(1032..1119)
                     /locus_tag="PLG98_03880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Leu,seq:taa)
     gene            1546..2967
                     /locus_tag="PLG98_03885"
     CDS             1546..2967
                     /locus_tag="PLG98_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOE32422.1"
                     /translation="MAFLIAFFIVSFVEVLRSSLAPSGAPEIPAASALEGTTPGSFSD
                     LTERVKPAVVNISTTKTYKGRTLGNPFGRSPFNDYFGDDFFNRFFGDIPQQDLKQRSL
                     GSGFIISNDGYIFTNNHVVENTDKILVKISDGKEYEAKIIGTDAKTDIALIKIKPDNG
                     LPTVEIGDSDAVRVGEWVIAIGNPFGLEQTVTAGIVSAKGRVIGAGAYDNFIQTDASI
                     NPGNSGGPLFNMQGKVIGINTAIVAQGQGIGFAIPINMAKNILNDLKTKGKVTRGWLG
                     VSIQNISEDIAKSMNYKGKSGVLVSDVFKGDPADRAGIKVGDIIVEINGKSIKDTHEL
                     LLTTAGLHVGEKAIIKVIRDGREMSFSIIVAERKDKPETALTQKSGGYFGIHAQEITK
                     DMAKQLGIAANTGVIVTDVEGGSPADNVGIQTNDVIIQVNKIKIISLKQYLAEISKAA
                     EKKNVVLLVKRGNVNFFVGLSVE"
     gene            complement(3071..5260)
                     /locus_tag="PLG98_03890"
     CDS             complement(3071..5260)
                     /locus_tag="PLG98_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOE32423.1"
                     /translation="MVKIDILEKAKSEKRNTLTEAEAKEVLKQYGIPVVQEKAVKTIA
                     EAETESATIGYPVVVKGLGAKLTHKTEKGLVKLNLNTREDVRNACIYIKEAAGDDLEG
                     FLIQPMLDGKREFVAGLFRDEQFGPTVMFGLGGIFTEALGDVVFRIVPFDEKEALKMI
                     DELNAQKLLGAFRGENAPDKKALVKTLVGLSRIGLEIPEIKEIDINPLLVSADGKVTA
                     VDALIVLGKAAERKKMVRQVDLKHIWKLFHPRSIAFVGASSKIGKWGHIMYAGVVAGK
                     YQGAIYLVNATGGEIAGRKVYSSVAEIPESVDLAVVTVPADKILSLIPQFKEKGINNV
                     LIITSGFSEVGSEGKILEEELVKKAREAGIVIVGPNTMGICNPYISFYCIGSHVRPPA
                     GGTSLLAQSGNLGTQLLAFAAKEDIGIRAYCGSGNEAMLTIEDYMDGFEVDEKTKNIV
                     LYIESIKNGQRFVETARRVGKHKPVIVLKGGRTKAGSSAAASHTGAMASDIKVFTAAC
                     KQAGIIQAESPMDLLDLSAAFSSLPLPKGSSVALLTLGGGWGVVASDLCEENNLIVPK
                     LSEDIIAEINPLLPPFWSHANPIDVVGDMNSETYMKILESLSRWSGCDAIIHMGIIGR
                     KVMIRSIMESTAAVDNKYDSNFVEGSLQYIEAFEKRTIEQTVQLMEKYNKPIVGVYLL
                     SDDQTRTVTNVNGCKYKGVNFVTPEKAVKALAKMYQYSKWLKCENEN"
     gene            complement(5309..5902)
                     /locus_tag="PLG98_03895"
     CDS             complement(5309..5902)
                     /locus_tag="PLG98_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE32424.1"
                     /translation="MDKPLMPQKLKQRLYPVVLRQFAGKDFHAVNIRDISKESGISTG
                     TIYKYFLSKEDLLFSIIDEKLLDLASLLRMHLEGTEDIREMFRKLFWVTMNFFDNNPD
                     LAVTAFITVPSKSFMASKSYKRESEIDILNGVVEKAKKRGAVISEIENRYFADLYMMI
                     TQRHIQNWYVHGMKWKLADTINQFFDFFWRVVKPIST"
     gene            6091..6600
                     /locus_tag="PLG98_03900"
     CDS             6091..6600
                     /locus_tag="PLG98_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOE32425.1"
                     /translation="MAENNISQIRINGRLVGIADLDHAIAKAARLDSKDDIDIKKCLL
                     NEISVNNYIPPSAQNAYADALLREFKIAHGLPVAEELFEGMNIEVVGMGCARCNQLEI
                     DVRDLLSEMNITAGLRHVTDFKEIARYNLLGSPALVINNKVVSVGDVPPKSKIRQWII
                     EAHQRTGKD"
     gene            complement(6613..7386)
                     /gene="mutM"
                     /locus_tag="PLG98_03905"
     CDS             complement(6613..7386)
                     /gene="mutM"
                     /locus_tag="PLG98_03905"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOE32426.1"
                     /translation="MPELPEVETLCRQLQKKIAGRSITGGKVYDDKLSAVQSFKKGKA
                     INVQRQGKTIVLELDNGRAINIHLRMTGRLFWREVSLRPDYARWRLSFDKGYLYLVDP
                     RRFATVTIGDIKKDTIKNDLMKDFDEKEFMSRQAKRSVSIKTLLLDPKAVAGIGNIYA
                     SEILYASGIHPFRKASDLSEGEWEKIITSSRQILKKGIEKRGTSISDWRDLYGRPGDN
                     QHALKVYGREGTHCSKCGGEICRIKQAGRSTFYCPDCQK"
     gene            7902..9209
                     /locus_tag="PLG98_03910"
     CDS             7902..9209
                     /locus_tag="PLG98_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOE32427.1"
                     /translation="MESETLFKEEEEPPGHLTGLVHVKNNVSDFLLFKDEVLPYPVKQ
                     KSENVLFPFKDSARSRLFRSQFYPEITRFEWFDWRWQLRNTIRDADRLAQIIHLSDNE
                     HQAMAHHSGALPVSITPYYASLLNPDDPADALRRCVVPVVDECLHTEGEEEDPLCEDA
                     DSPVPGIIHRYPDRVLFLVTDICSTYCRYCTRSRIIERSTHHLINLEKWENAIQYIAG
                     NKNIRDVLLSGGDPLTLSDDKLEWLLARLHKIRHVELIRIGTKVPVVLPQRITPRLTA
                     MLKKYHPLWMSIHITHPRELTPEMSAACIRLADAGIPLGSQTVLLSGINDDVATMTRL
                     VQGQLQIRVRPYYLYQCDPIPGSSHFRTPISKGIEIIQGMRGHTTGYAVPTYVVDAPG
                     GGGKIPLLPEYAIGWENGSLLLRNYEGNIFRYPDNHAKSKDMH"
     gene            9248..10267
                     /locus_tag="PLG98_03915"
     CDS             9248..10267
                     /locus_tag="PLG98_03915"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32428.1"
                     /translation="MSRVSKKLKIGLTYDMRDDYLKEGYGLEETAEFDLPETIEAIER
                     VILDNGFQADRIGNIKALSRRLVAGDRWDLVFNIAEGMYGFGREAQVPALLEAYNIPY
                     TFSDPLGNAITLHKGFTKQILRDAGIPTPAFVVVDTAAHIDTISLPFPLFAKPVAEGT
                     GKGITALSKITSRKNLRAVCLNLLKTFRQPVLVETYLPGREFTVGILGTGKDARALGV
                     LEVILKPEAEKNAYSYENKEHYDKLVDYVLVDDPEARKAADISLMAWRALDLRDAGRV
                     DLRSNTYDVPHFMEVNSLAGLNPVRSDLPILCSKIGMSYHELICAIIKSALQRVKNHP
                     ENRCK"
     gene            10273..11274
                     /locus_tag="PLG98_03920"
     CDS             10273..11274
                     /locus_tag="PLG98_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32429.1"
                     /translation="MKKVVILHSDIATDGSLDELDCLVQAEAIADAVRTLHYEPVLLP
                     FELNLNRTITMLQSLKPAVVFNIVETLDSKGSLIHFAPAVLDVLQIPYTGCPTHAIYL
                     TSNKPTAKKIMRDAGIATPDWLEQNGIIFRQDDGGKFLIKSSWEHASIGLDENSLISY
                     TDKTAIIKEMKLREKKLGGSCYAEAYIDGREFNVALMSDQAGARVLPIAEMLFQDYAP
                     DKLKIVDYKAKWDADSFEYNNTVRKFNFDDEDALLISSLQDIALQCWNIFSLRGYARV
                     DFRVDKNNKPWVLEVNTNPCLSPDAGFAAALERAEIKYHEAIGLIIDNALKSISKEI"
     gene            11280..11627
                     /locus_tag="PLG98_03925"
     CDS             11280..11627
                     /locus_tag="PLG98_03925"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOE32430.1"
                     /translation="MLKTIIFLFFSNLFMTFAWYGHLKNFNHKPLLFVILFSWGVAFF
                     EYVLQVPANRIGFHYYSLGQLKVIQEVITMIVFAGFAIMYMKQPLKMDFLWAGLCLVG
                     AVYFMFREGLPHT"
     gene            11636..12121
                     /locus_tag="PLG98_03930"
     CDS             11636..12121
                     /locus_tag="PLG98_03930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE32431.1"
                     /translation="MKKFIYRQQIKPSDIEVIADMVESSGFFSDEEIEIAIELAEDKL
                     DKQEVSSYQFLFAEIENQVVGYTCYGLIPATAGSYDLYWIVVAKDLQGLGVGKMLMAE
                     TENRIYQNGGRQIYAETSSRAQYTPTHKFYESCGYAKEAFLKNFYSEGDGKIIYAKVL
                     K"
     gene            12260..12880
                     /locus_tag="PLG98_03935"
     CDS             12260..12880
                     /locus_tag="PLG98_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOE32432.1"
                     /translation="MRLFPKEENFFELFDELADKIEEGGKLFLEMAQTRDYSDVRVAK
                     LKEIEHEADGITHKTYEKMHKTFLTPIDREDIYALVNKMDSIMDVIEATAVRIHLYKV
                     KRTSDEIIKQAQILNDAIKKLKTIIQAMRNMKNSDMILAGCVEINTLENAGDITLRSI
                     MANLFETEKDAIELVKWKDIFQLLEEAIDVCEDVSNIVEGIVLKHA"
     gene            12873..13874
                     /locus_tag="PLG98_03940"
     CDS             12873..13874
                     /locus_tag="PLG98_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOE32433.1"
                     /translation="MLDSMAFVVFLIIGALAFDFLNGFHDSANSISTVVSTRVLSPKY
                     AVIWAAFFNFAAVFFVGTAVAHTVGKGIINIDIVDNLVILAALGGAIIWNIITWYYGL
                     PSSSSHALIGGLIGAAISKAGFSTLVWAGIIKTTVFIIVSPAIGMALGFIFMVTAMNL
                     SHNSNIGKSDKLFRKLQLLSAAVYSLGHGMNDAQKTMGIIAMALFSKGLLGDTFYIPV
                     WVIIACYSMISLGTMFGGWRIVKTMGTKITKLQPMGGFSAETAAACSIIGATVAGIPV
                     STTHTITGAIVGVGSTRRLSAVRWGVAGKIIWAWILTIPAAAILSSIIYLCVQLFIT"
     gene            13980..15245
                     /locus_tag="PLG98_03945"
     CDS             13980..15245
                     /locus_tag="PLG98_03945"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOE32434.1"
                     /translation="MPQKILPFSKKQIENIIKDYPTPFHIYDEKAIRDNARAFINAFN
                     WNEGFKEFFAVKAAPNPYLMKILHKEGYGADCSSLAELMMAQKTGITGEEIMFSSNDT
                     PVEDFLLAKKLKAIINLDDISHIAFLEKSAGLPALICFRYNPGKLKKGNHIIGHPEEA
                     KYGFTREQLFEGYKICRDKGVRRFGIHTMVASNELDPYYFVETAEILFNLIVELSQKL
                     KIKFEFVNIGGGVGIPYRPEQKSIDVNVMSRGIRKKYNDIIVANGLAPLKLFTECGRY
                     ITGPYGYLVSRVRHIKNTYKKFAGLDSCMANLMRPALYGAYHHITVLGKERKSHNILY
                     DVTGSLCENNDKFAIDRKLPKLEQGDILVIHDAGAHGFAMGFNYNGKLRSAELLLRAN
                     GKVVQIRRAETIKDYFATLDFNGLKDFNA"
     gene            15407..16966
                     /locus_tag="PLG98_03950"
     CDS             15407..16966
                     /locus_tag="PLG98_03950"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32435.1"
                     /translation="MASFKDIFNAAEQGNPAAQYNLGLIYRKGEAVPQDDAEAVGWFL
                     KAAQQNHAKAQLNLAWMYQEGKGVSQDDVKAAKWYLKAAEQGNAQAQFNLALMYSKGE
                     GVALDHIEAAKWFLESAEQGIANAQYNLGIMYQDGLGVQKDSTEAVKWFNKAAKQGHT
                     DAQAKLDAMRKALQDEQREAARAVKESPRILPSNAADASSIPDEFPDEKKEALSQDHA
                     KQILVSQYLKAAEQGLADAQYHLGVIYEEGSGVPRDNEKAIKWYRAAAEQGHPEAQNK
                     LEALSPAGHETMPGDTQPPVDGNETTAYTKPDESANALIVVTDELAEQNHDIEQEVPE
                     TTATESNPSPEISDDEKGYREAAEQGDADIQNRLGLMYKNGDGVMQNHVEAAKWFLKA
                     SEQGFADAQLNLGNMYQEGSGVPQNDAEAAKWYREAAEKGHAGAQYNLGLKYFFGEGV
                     PRDFAEAIKWYQKAALQDNVDAQFTLGGIYEEGVVIPEDPAEAERWYRRAAEKGHERA
                     RYRLELMHRHV"
     gene            17314..17955
                     /locus_tag="PLG98_03955"
     CDS             17314..17955
                     /locus_tag="PLG98_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32436.1"
                     /translation="MLKISRHIHEKRFPQILTLFFTLILVCLTMVWSADPAYSNDTQD
                     KLYSLNLYYGEISKNHADDFILDTGTLRLTDCRLAAVTLARRLYAYKDLANLEVEGQI
                     VKHFYDIQDHWEFNAIVTARWNAFFWDKYVDTSIAFGLGPSYATEKPEMEAILDRETS
                     QWLAYWMVELEMGLPAHPGISFITRLHHRSDAFRIVSPGGASTAPVVGLKIRF"
     gene            18193..19038
                     /gene="murI"
                     /locus_tag="PLG98_03960"
     CDS             18193..19038
                     /gene="murI"
                     /locus_tag="PLG98_03960"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOE32437.1"
                     /translation="MNTKANRAIGVFDSGIGGLTVVRALMERLPFENIIYFGDTARVP
                     YGVKSVETINRYAAQITEFLIQKDVKMLIVACNTMAAVAHQTIIDLSQVPVAFAYQKL
                     VEQVPVLEVIDASARMAIQHTKTKSIGVIGTPATVNSNAYARAIHLLDKEAKVFSHAC
                     PLFVPLVEEGWFDHPATKLIAQEYLKQVIAEQVDTLVLGCTHYPLLKPLLQDIMGKSV
                     TLIDSAEAMAGIAADLIDQQAIGNQNHAVPEYTFYVSDLPYRFQTIGERFLGRTLGKI
                     ELVSF"
     gene            19048..20511
                     /locus_tag="PLG98_03965"
     CDS             19048..20511
                     /locus_tag="PLG98_03965"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOE32438.1"
                     /translation="MKILLAGHNLDYDIIRTLKDKSGTKQDLTPETISAAYARISRNP
                     KPVNELRAIAREEVDKARQSNRKIVFDMGHNSVAEHAVFNIDVIGVSRFLVEEIEKFR
                     LCSFTEKSQRYVLFDKDFVVPEEIRQADLAGLFISTVEAQNDFYHEMYENLRPYIFDK
                     HKTLASDPANRSMLEGWAKEDARYAISMATETQLGMTVNARNLELMLRRLAALPLAEA
                     QEYSAKLYAATKEIAPSLIRYTKATDYDKFTRRNLNRFSDALIKKSQSKVKNAADVQL
                     MSASAAADNRAAAALLFASSDANYAECLKTADKLTAQKKRSLFKTAFANLQIHDAVLR
                     ELETVDLQFELIASSSCFAQLKRHRMSTIIAQEYNPHLGVTVPPSIKAIGRQKDFLDL
                     MHRTQSTYEKIREKAPLAAVYVLTNAHRKRVLMKLNAREMYHLARLRADAHAQWDIHN
                     LTGKMLKQAKKVMPLTLMMACGKDHFPSLLNKTYSCT"
     gene            20535..21929
                     /locus_tag="PLG98_03970"
     CDS             20535..21929
                     /locus_tag="PLG98_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE32439.1"
                     /translation="MKINANKNHLYVLLVTTLSSFLTPFMGSAINVALPAIAEELSMT
                     ALTLSWVATSFILAASISLIPLGRLADIYGRRRIFIYGALIFTVASAFCIWSPTQAFL
                     IAVRALQGIGGAMIFSTGTAMLISAYPPSDRGKILGINITAVYVGLTVGPFIGGLLTQ
                     YLGWRYIFLFTFFLGLTIIFITLAKIEPETSAREDEGFDLTGALIYAIALFSIMYGFS
                     HLPAFSAGCLIAIGMIFLGLFIFQQLKSPFPLLDIRLFLDNKVFAFSNLAALINYSAT
                     FVINFLLSLYLQNIKVLTPSEAGSILIAAPIMQALISPFAGRLSDRFEPQIIASIGMS
                     ITVAGLVLLIFLNGSTGIHYVLFCLVLLGIGFALFSSPNVNATMSSVENKFYGIASAT
                     LGTMRLTGQMFSMGITMLIFAVILGNHPISAANHHLFLKSTKLIFSILSILCCAGIFA
                     SLARGKMHEGNHSL"
     gene            21907..22677
                     /locus_tag="PLG98_03975"
     CDS             21907..22677
                     /locus_tag="PLG98_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOE32440.1"
                     /translation="MKAITLSSCIIGLVAGTFGGLIGIGGGIIMVPLMVEILKLPQHK
                     AHGTSLVALVFTGISGSITYALNGSIDIKAAALLALTAIMTASLGARYAGSLTEQKLK
                     KAFGAFLLFCAALLFLKLYLPNVVVSDDAKPVIYLTTGALTGFLSGMMGVGGGTIMIP
                     VMVILAGFTQHVAQGTSLLVMIPSGAVGALTHWKMGNTEQGILWGLIPGILLGTYIGG
                     TVANIIPNDTLRLIFAGMLIFLGIRYFGMSSPRRIGKG"
     gene            22680..23699
                     /locus_tag="PLG98_03980"
     CDS             22680..23699
                     /locus_tag="PLG98_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32441.1"
                     /translation="MKKTDGYRKFSFFTDVRGMKNAIGEMRGWGHPHQPIFNAGYQRL
                     PDDPVFNEDWFISTIKEKIATHPENTMEYPFYGEKIFTEPLIGFIQGDDPIFQQYKTI
                     IGPHHFTPEEIMQWQARQHGVPAPRAEDLSVVSFILPLTKITKNDNAAMKEWSAERWA
                     QTRLLGEIFSQIFVREIVTCLMKRGILAIAPDVTPMFNKKRYPKVGWASPWSHRHMAY
                     AAGLGTFGMHDFFITEKGCAHRTGSFVVNVKLTSNRERPDDIHAHCLQYQGKSCMQCA
                     KRCPVNAITAENGHNKEVCYQRVAKSLIHCNKNYHIFIYGCGLCATGVPCESEIPKIL
                     QKNSK"
     gene            complement(23843..24955)
                     /locus_tag="PLG98_03985"
     CDS             complement(23843..24955)
                     /locus_tag="PLG98_03985"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOE32442.1"
                     /translation="MIKSRLCDMTGIKYPIIQAGMGPFSNNNLGVAAANAGVLGLFST
                     SGLCNKDFLPFIYNDWIKSAEANPDDDIPTAIEKLLKRAYRLTKDTNGVFGINVMVSA
                     ELLFQAELIINAAIQVRAANPGMEKHFKVIFTSAGDPVPWKEKIKGAGFTWMHVVPSV
                     KGALRCKKAGVDVIVASGHEGGFHTSWEPVHSMILLPAVVEAVSDEKTLVCGAGGFCD
                     GKTLAAALVMGADGVQMGTRFLATQESDFPPIWRQGVVEAGDRGTLVARGFVGPARWL
                     KNPRSEEHAQNTLQKSPGCYLGTPDDMLSIDFKLIEFEMESMQAAYDGNKEKALLAAG
                     EAAQRINDMPKVADMVQSMMKETEDILKGVQTKMLA"
     gene            complement(24995..25999)
                     /locus_tag="PLG98_03990"
     CDS             complement(24995..25999)
                     /locus_tag="PLG98_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOE32443.1"
                     /translation="MKKNSKKSIWVFGDYRNYYQNRVTLQLIARAVALAKVIDAEVCA
                     LIFGHEIDEWIMEYTAHGADRILAIDDPALKSYSTEKYLAIIERLAGERDPEIILIGA
                     TDFGREFAPRLAKRLKTGLSADCVGLDIADDGLLLQIAPSFGGNMLAEIVTERHRPQM
                     ATVRPGTFKEIPHDNQRKALVERLPLMKNLPKPRVRLIEYERSVEKEHTIENASVIIC
                     GGRGMGNKEKFKKLQELARLLGGDVGATRPVVYAGWADHDALVGQAGKHIKPKLLFSF
                     GISGAIQHTAGLGEAHFIIAINKNPQATMLKMADVAIIGDASDVLNHMIRELKKRIRE
                     "
     gene            complement(25986..26822)
                     /locus_tag="PLG98_03995"
     CDS             complement(25986..26822)
                     /locus_tag="PLG98_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOE32444.1"
                     /translation="MLNLVVCLKQVPMVTELPWDEKTGTLRRDLAAGMMNPACKHALE
                     AALQIKEHHPANITVITMGPPAAEEVLRESLALGADQAVLVCDRLLAGSDTFATSLIL
                     KEAIKKKCPKFDLILCGAYTADSETAQVGPQLAEELDLPAAAYVEKLEISGKTLRVRR
                     LVDNFRETLEMEFPALVTISMENYKPRYTGLAGLESAFDKNDVVILDAKALGLTAASI
                     EKKGSRTKVRKVFLRKTQKGNVLLQGAASAVVTEFLDKYQRKIGTVIGKSIEEKKSDE
                     KK"
     gene            complement(26825..27976)
                     /locus_tag="PLG98_04000"
     CDS             complement(26825..27976)
                     /locus_tag="PLG98_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE32445.1"
                     /translation="MQLNPCAKHKLIRDSVRTFAETELAPVASAIDRAATFPREIIEK
                     MRPLNYFGLQVPREFGGAALDSVSAAIVVEEISRVCAAIGLCVTVHNGVAVYPFLRFA
                     NAEQKKHYLPKLAEGELIGAFSLTEANAGSDSASVETTAVKRNDAYVLNGTKIFVTNG
                     GVCDLALIFALTGSEQGKPQSSVFIVESKYPGFLRGELEDLCGMRANPVSSLFLEDCC
                     VPENNVLGRIGDGMKIGLATLDNGRIGVAAQALGIAQGAFEAAVKYAKERQQFKKPIA
                     SLQTIQNYIADMATEIAAARLLLYRACSLKDEGMPFGCEASMAKLYCSTVASRVTSLA
                     VQIHGGYGYSKEYDVERYFRDAKVTEIYEGTSEIQRMVIARSILSQLIL"
     gene            28131..29501
                     /locus_tag="PLG98_04005"
     CDS             28131..29501
                     /locus_tag="PLG98_04005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOE32446.1"
                     /translation="MTLYFYAMVHREAKNKSSRKTKAVHHDPDALPSLMANPQEKLAV
                     SLPLRLIIRLALGFVDLFHIWRFPRKTDGCFEHMTLIDKLHWLYKSANPIRLAEKKSS
                     LEDFFAGQAETSLTLPPDFEQTTQIQLAAVGDLMQNDLLTHSADSLYEGIAAELFTAD
                     ISFANLEAPLTTQPQRNLLFVRSETPSTYISAGQFQVLKGYKDQKFTVFSTAGNHAWD
                     YGEEGVLSTLDALAKEGIVAVGTNREKADRQKAAIIDVQGIRIGFIGLTFGLNGKKID
                     PEQEYHVNLVQLNALSAEVDLSVPIRQINYCREQYCDFIVGSLHWGLEFEFFPARRQL
                     AVAHQLAEAGLDLIISHHPHVVQPVEFYRTRRDPQRTVVITYSLGNMVSPFHDDDLNL
                     SFLLQVTLADGKLKNQKRTYIAACRIIPVCQRALMAGGKPTVRLEKVTRLAAFRLPFD
                     LKNLAP"
     gene            29694..30176
                     /locus_tag="PLG98_04010"
     CDS             29694..30176
                     /locus_tag="PLG98_04010"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOE32447.1"
                     /translation="MSKEKIPTTPAVLALKAQQADFTLHTYPYEERGGTKASARKLGV
                     DEHMVIKTLVMEDETGKPLIILMHGDREVSTKSLARQIGAKSVTPCKPAVAEKHTGYK
                     VGGTSPFGTRKVLPVYMEQTIADLPDILINAGSRGLLARMPSAELARLLKPVMVGVAI
                     "
     gene            30226..30783
                     /gene="trxC"
                     /locus_tag="PLG98_04015"
     CDS             30226..30783
                     /gene="trxC"
                     /locus_tag="PLG98_04015"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOE32448.1"
                     /translation="MPDDVIIRCLNCGTKNRIPKQKLQGRSICGKCGASLDELIIRCL
                     KCSTKNRIPEDRLNQRPLCGKCHEPLIIPQQKVLPFDVTDQTFAREVLTSDSSVLVDC
                     WAPWCGPCRTLSPIIDELASDYANGVKVVKLNVDENPLTASQYGIRSIPTMLFFKEGK
                     MVHRVTGALPKEEIEQHLLTIIKTN"
     gene            30799..31335
                     /locus_tag="PLG98_04020"
     CDS             30799..31335
                     /locus_tag="PLG98_04020"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE32449.1"
                     /translation="MADNKSSIVYQCKIFDVREEEVTFPNGKTARQSWINHHPTVAIV
                     AVNEKNELLLIKQYRNAVKQDLLEIPAGSLDGIEESPAVCAQRELAEETGFKAKSLIK
                     LYEGYLLPGYCNEYMYFFLALDLFSAPLNPDDDEFIETIPATFSQAHKLIDRGVIIDA
                     KTVLGILLAEKLLGRQNA"
     gene            31436..32734
                     /locus_tag="PLG98_04025"
     CDS             31436..32734
                     /locus_tag="PLG98_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE32450.1"
                     /translation="MIYNEEFETMPREVIKALQVKRLQQVLERVYHTVGFYKKSFDAA
                     KVKPDDIRCMADMKKLPFTTRKDLRDNYPFGLFAVPMSSVVRLHASSGTSRRSAVFGY
                     TKRDIDTWSDLIARSLVAAGITKNDIIHNAFGYGLFTGGLGLHYGAEKIGASVIPISG
                     GDVKRQLMILQDFGPTVLCATPSYALHLAEEGNAMGIDIKALKLRVGIFGAEPWTDAI
                     RDDIEKAFGITALDLFGLSEVIGPGMAMECLEGRHGMHIFEDHFIVETIDPQTGDVLP
                     EGAEGELVFTTLTKEANPLVRYRTGDISRLITEPCRCGRTHIKMERVLKRSDDMLIIR
                     GVNVFPSQIEAILVEIEGLEPNYQVIIDKAGALDVLDLQVEVNDKIFSDSGSIKQLQK
                     IEQRIVKDMRDYLGVSARVKLVQPNTLKKTDAKIIDKRRI"
     gene            32757..33188
                     /locus_tag="PLG98_04030"
     CDS             32757..33188
                     /locus_tag="PLG98_04030"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOE32451.1"
                     /translation="MKVEQISVFLENKPGTLEDATRVLKEANINIRTLSVAETVDFGI
                     LRLIVNDVEKANKVLKDNGFRVSKTPVVAVEVPDTPGGLHSIMEVVSKEGINVEYLYA
                     FVEKSGQNAVIIFRFDNPEKAIDVLTKKNFTVIPGSKLYEF"
     gene            complement(33504..34391)
                     /locus_tag="PLG98_04035"
     CDS             complement(33504..34391)
                     /locus_tag="PLG98_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE32452.1"
                     /translation="MPVEKTRTTHILLSVFHGIPRCVRKGMFLSIFFLFYHIGTKSRL
                     ITLHNLMRSFPEKDTKEIIKIAKGVYRHFAIVVAEFFDLPYMTKDNIHKWVDVEGVEN
                     FQAGIAKGKGMLSIVAHFGNWELMTIAGPMYLKPMYIVYRPLDNHVIDNIVEYVRTMQ
                     GNDMIPKGGSGKRIMELLRENNLIGILSDQNVAHYEGVFVDFFGRTACTGVGLAVMAM
                     RSGAPVLPAFMARQKSGKYKFIIKPTIEAVCTDNYENDLVVNTQRFTKIIEEVIREYP
                     DQYFWFHQRWKTKPWQKKM"
     gene            complement(34404..34706)
                     /locus_tag="PLG98_04040"
     CDS             complement(34404..34706)
                     /locus_tag="PLG98_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32453.1"
                     /translation="MTEIIFDENEDWEKRVLCSDESCIGTIGPDGKCKECGKPYEGKL
                     PEGKINEIVHEVSSEKPDSSDDHSDDDWDKRVLCSDGSCIGVIGPDGKCKECGKPL"
     gene            complement(35328..36770)
                     /locus_tag="PLG98_04045"
     CDS             complement(35328..36770)
                     /locus_tag="PLG98_04045"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOE32454.1"
                     /translation="MSDIKLKKLDDNRWLLPQTGGMRVPGIIYANEKLFQLLKGDESA
                     KQVANVAHLPGIVNFSLAMPDVHWGYGFPIGGVAAFDLDGGIISPGGVGYDINCGCRL
                     MTTNLTLGDIKNRLSDLTTALYQRIPSGVGSTGSVKLSGKDQKKVLEEGAAWAIKAGF
                     GLNEDLETTEDGGAISGADPELVSARALERGKQQLGTLGSGNHFLEIEVVQEIFDEEA
                     AKAFGLHKGQIAVMIHTGSRGLGYQICDDYLALMVKHANETGIALPDRQLACAYLSSG
                     RGKNYLAAMACGANYAWANRQILMHKTREVFEKTLRMSPRDVNMRLVYDVCHNIAKIE
                     SFNIGGVIKKLCVHRKGATRAFPAGHEAVPVRYRKVGQPVLIPGDMGTGSYVLVGQQK
                     AMSETFGSACHGAGRVMSRSQAIKNSRGRSIARELSEKGVLVMAGSKGTLSEEMPEAY
                     KNVQDVVQVMHEAGIARKVARLKSLSCIKG"
     gene            complement(36911..37450)
                     /locus_tag="PLG98_04050"
     CDS             complement(36911..37450)
                     /locus_tag="PLG98_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOE32455.1"
                     /translation="MTVKQSANTYCLTQDQLVMKVAEQSALLVQYARFSYTHSMSNYK
                     FFDHTADIGVEVYGRTRKELFMNTAGALFDVMIEHKAGRATAKRQKKITVEGTDVADL
                     LINFLRELLHLFNGEHFITGSCEIIEFSNKKLQARLTGESFNNKKHSIKTEIKAVTYS
                     GATVKRVESGWKARIIFDV"
     gene            complement(37655..38407)
                     /locus_tag="PLG98_04055"
     CDS             complement(37655..38407)
                     /locus_tag="PLG98_04055"
                     /inference="COORDINATES: protein motif:HMM:NF037888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32456.1"
                     /translation="MPTHIERLEAHASHLLDAFIQLRERYALLEPMLFDESMPKERCS
                     GNQARGFCILKHSLFLSCSQDIANLTLDDDDKTPSLHNLIRAITDDVIRKELQNRFAI
                     WKIPSIEEQTEPEIIEALHRIELREEAERREQFEKLYCEAMAIWAELSTSKKIKAFRI
                     VRDKVSAHKEVRYVADKYQFVDIGTLGIKWGDLRSTIDRMKRLVELMGLLVRNAGFAW
                     DMLDEQLSRASKGFWDISNAAEESIESGERKQ"
     gene            complement(38480..38836)
                     /locus_tag="PLG98_04060"
     CDS             complement(38480..38836)
                     /locus_tag="PLG98_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32457.1"
                     /translation="MNKLRSLFSIKGIVLSCSAFFFCYARHIIQYKNPPWQNGFLGFL
                     GSWFSHYIVILLIAVIVLAIVKNCGRYLFIDKEPHLDNIEDTVIHVSLTILAASLLLL
                     FAYYVGPHVYVDDIYE"
     gene            complement(38985..>39379)
                     /locus_tag="PLG98_04065"
     CDS             complement(38985..>39379)
                     /locus_tag="PLG98_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOE32458.1"
                     /translation="IPAQNTFMLTVSENGFGKCTPVDEYRVQSRGGKGTINLKTVEKV
                     GNVSGVLQVVGEDNVMLISSAGKVIRLKVQEIPVNHRVTQGVKLIDLEPEEKLVGVAR
                     TTSEAEGKDDGNGDDEADTNGEDNGAEE"
BASE COUNT        10257 a         9901 c         8811 g        10410 t
ORIGIN      
        1 tatttaaata ataaattaac ttttattgca atgaggccgt aattgagttg tataaaaaca
       61 ggaaaatctc attatttaat attgcataga cttttgttca aaaaagcgat ttcctaagtt
      121 taattccttt catcaagatg aaagtcatca gatataataa ttaaaaattg gtcaagattt
      181 tactagtggt ggaacggagt ataagaagaa tgcacaggta tgtcaattaa attataataa
      241 aatgtaaagt gttgagcata tgtttgtcat attgcagtat tgtatttttg tcgagattga
      301 caatcagttg gcagggttct ttgtctcacg ctatatagcc aagttcaagc attgctgcca
      361 ttcttgccac atcctcaaat ccgtgataat ctatttaaaa tggtgagtgc gaagtctggc
      421 caccatttcg cttttatatt ggtataataa aaatataaac caaataattt ttaaaataca
      481 tggccataat acgaacggct ttttagacgg cttttggttc ccataaacgg atttaaacgg
      541 ccttaaaagc gtgtaaaatc aggtggaata cgctcttagt cgccctctca cgccggaaac
      601 cggggttcaa gtccccgtgg gactaccact aacaccgttt gagtattaac aagcgtcatt
      661 ccctctcata cattattgat tgtatcgcta gacaaatcga aaaaatgttt gatgctttgt
      721 cagtgattgc attcgcatct ttattgtgtg gtctttcact gtgtggtgtt tggtttttga
      781 ttcttctgct attatcaaaa cgggtctgaa gtttgagttt agaactcttg tttcaattca
      841 acgatagagt ttatctacga ttccgggatt tattccctgt tttcctgaaa gaaaaaaagg
      901 ctcgccggca aaaaagtttt ctaccatttt atgatctgca aaacatggtt atgcgatgtt
      961 gcgtgattaa ttcgttgtta aaggatgttt tttttaatcc gcaaaaagca gaagggtatt
     1021 aaaaagtgat ctggtgcctg gggcgggaat tgaacccgca cagcctcaag gaccgaggga
     1081 ttttaagtcc cttgcgtcta ccagttccgc cacccaggca tgaattttat aatttaaaaa
     1141 ttcgtcaagc ttatattatt tttgaaattc actgtcaagg atttgctggc atacgattca
     1201 aatatctata gaaatttttg atttgattta aatctctaac aattaaaatc tataaaaaaa
     1261 ttttcttcag atggactaat tttatacagc aaaatatttt cgccgacggc agataatgat
     1321 ccgtttgttt ttttaaaccc aaaacgtttg aaaagatgtt tcttgctgac ccatattaaa
     1381 caatatttta ttgacaaaaa catctttgca gttatattgt ttaaaaagta taaatttaat
     1441 atatcgcgta aatctatcat caacaataat atttgaccaa taataacgga ggtttataac
     1501 attaatatgg aaaataagaa ccgtaaaacg tttttcatgt ttttgatggc gtttttgatc
     1561 gcctttttta ttgtctcatt tgtagaagtg ttaagatcgt ctcttgcccc ttcgggcgca
     1621 ccggaaattc cagccgcatc ggcactggaa ggaacgacac ccggatcatt ttccgatctg
     1681 accgaaagag tcaaaccggc tgttgtaaac atcagcacga caaaaaccta taaaggaaga
     1741 accctgggta atccgtttgg gcgttctcct tttaatgatt attttgggga cgacttcttc
     1801 aatcgtttct tcggcgacat tccgcagcag gacctcaagc agagaagcct gggatcaggt
     1861 tttattatca gcaatgacgg ttacattttc acaaataacc atgttgttga aaatactgat
     1921 aaaattctcg taaagatatc agatggaaaa gaatatgaag caaaaatcat cggcacggac
     1981 gcaaagacgg acattgcttt aataaaaatt aaaccggaca atggattgcc gaccgtggaa
     2041 atcggagatt cggatgctgt aagagtggga gaatgggtta ttgccatcgg caatcctttt
     2101 ggacttgaac aaacagtcac ggccggaatt gtcagcgcca aaggaagggt gatcggcgca
     2161 ggtgcttacg ataatttcat tcagacggat gcctccatca atcccggaaa cagcggagga
     2221 cctttattta atatgcaggg gaaagtcatc ggcatcaata cagccattgt tgcacagggc
     2281 cagggcattg gctttgcgat tcccataaat atggccaaaa acattttgaa tgatttaaaa
     2341 acaaaaggaa aagtcacccg cggatggtta ggggtttcga ttcagaatat ttccgaagac
     2401 attgcaaaga gcatgaatta taaaggcaaa agcggtgtgc ttgtttctga tgttttcaaa
     2461 ggcgatccgg cagatcgagc cggcataaaa gttggtgata ttattgtaga aattaatgga
     2521 aagagcatca aagatactca tgagctattg ttaaccactg ccggtctgca cgtaggagaa
     2581 aaagcaatta tcaaagttat tcgtgatgga agagaaatgt ccttctcaat tatcgtggcg
     2641 gaaagaaaag acaaacccga aacagctttg acacaaaaat cgggtgggta cttcggtatt
     2701 catgcccagg aaattacaaa agacatggcc aaacaattag gcattgcagc caatacgggt
     2761 gtcatcgtta cggatgtaga agggggcagt ccggctgata atgtcggaat tcagaccaac
     2821 gatgtgatta ttcaggttaa taaaattaaa atcatttcac tcaaacaata tctggctgaa
     2881 atatctaaag ctgcagagaa gaagaatgtg gtccttttag tgaagcgagg caatgtcaac
     2941 ttctttgtag gattaagcgt agaataatat aatttatatg atattaaaaa aggcaggcta
     3001 tggaaagcct gccttttttt gttacagggc aaaaatgttt cacgtgaaac atttcttgtc
     3061 ctccatgatg ttaattttca ttttcacatt ttagccattt tgaatattga tacatttttg
     3121 ccaatgcctt gacagccttt tccggggtca caaagtttac tcctttatac ttacatccat
     3181 ttacatttgt aactgtcctg gtttgatcat cgctcaacaa ataaacccca acaattggct
     3241 tattatattt ttccattaat tgtaccgtct gctcgatcgt tcttttttca aacgcctcga
     3301 tatactgtaa agagccttca acaaagttag aatcatattt attatccacc gcggcagtgg
     3361 actccataat cgatctgatc atcacctttc tgccgataat ccccatatgg atgatggcat
     3421 cacatccgga ccatcgactc aggctttcca gaattttcat ataagtttcc gaattcatat
     3481 cgcctacaac atcgatggga ttggcatgac tccagaaggg cggcaataat ggattaattt
     3541 cagcaatgat gtcttctgat aattttggaa caatcaaatt attttcctcg cacaagtcgg
     3601 aggcgacaac cccccatcct ccgcccaacg ttaaaagagc gacactactg cctttgggca
     3661 acggcaatga ggaaaatgct gctgaaaggt ccagtaaatc cattgggctt tcggcttgta
     3721 taatgccggc ctgtttacac gctgctgtaa acactttaat gtcggaagcc attgcaccgg
     3781 tatgactcgc tgcagcactg gatccggcct ttgtgcgtcc gccctttaaa acaataaccg
     3841 gtttatgttt gcccacacgt ctggcagttt ctacaaatct ttgaccattt ttaatgcttt
     3901 caatatataa gacaatgttc tttgtcttct cgtcaacttc gaatccatcc atatagtctt
     3961 caatggtcaa cattgcttca tttccggaac cgcaataggc tcttattcca atatcctctt
     4021 ttgcggcaaa agccagcaat tgcgttccca gattaccgga ttgggcaagc aatgaggtcc
     4081 cgcccgccgg tggccggaca tgcgaaccta tacaataaaa tgatatatat ggattacaga
     4141 tgcccatcgt gtttggacca acaataacta ttccggcctc tcgcgccttc ttaaccagtt
     4201 cttcttccaa aatctttcct tctgaaccta cttcactgaa tcccgacgtg atgattaaaa
     4261 cattgtttat gcctttttcc ttgaattgcg gaatcagaga taaaatttta tcagcgggaa
     4321 cggtaacgac ggcaagatct acagactcag gaatttccgc aaccgacgaa taaacctttc
     4381 taccggcaat ctctcctcct gtggcattaa caagataaat ggctccctga tattttccgg
     4441 caacaacgcc ggcatacatg atatgccccc atttgccgat tttcgaagaa gcacccacaa
     4501 acgctattga tcgtggatga aataatttcc agatatgctt taaatcaacc tgacggacca
     4561 ttttttttct ttccgcagct tttcctaaaa cgatcaatgc gtcaactgca gtcacttttc
     4621 cgtctgcaga aactaataat ggattaatat caatttcctt gatttccggt atctctaaac
     4681 cgatacgcga caaaccgacc agtgttttca ctagagcttt tttatccgga gcgttttctc
     4741 cccgaaacgc tccaagcaat ttctgcgcat ttaactcatc aatcattttc aacgcttctt
     4801 tttcatcaaa aggaactata cgaaaaacaa catcgccgag tgcttctgta aaaattcctc
     4861 ccaaaccaaa catgacggta gggccaaact gttcatcacg aaaaagtccg gcgacaaact
     4921 cccttttccc gtccaacatg ggttggatta aaaatccttc caaatcatca ccggctgctt
     4981 cctttatata aatgcacgca tttcgaacgt cttccctcgt gttcaagttc agcttaacca
     5041 gacctttttc cgttttatgc gttaattttg ctcccaatcc tttcacaaca acaggatatc
     5101 ctattgttgc cgattcggtt tctgcttctg caattgtttt aaccgctttt tcttgaacaa
     5161 ccgggatacc atattgtttt aatacttctt tggcttccgc ttctgttaat gtatttcttt
     5221 tttctgattt tgctttttcg aggatatcga ttttcaccat tattgactcc gtattaccgc
     5281 atgctgattt tattatttat taatattttt atgtacttat cggttttacg actctccaga
     5341 agaaatcaaa gaactgattt atcgtatccg caagtttcca tttcataccg tgtacatacc
     5401 agttttgtat gtgtctttgc gtaatcatca tataaagatc tgcaaaataa cggttttcaa
     5461 tttccgatat aacagcacct cgcttttttg ctttctccac aacaccattc aagatgtcta
     5521 tttccgattc gcgtttatat gatttgctgg ccatgaaact ttttgacgga accgttataa
     5581 aagcagtaac tgccagatcc ggattattat cgaagaaatt catcgtcacc cagaacaatt
     5641 ttcgaaacat ttcacggata tcctccgttc cctcaagatg catccttaat aaggacgcca
     5701 ggtccaataa tttttcatca atgatggaaa acaaaagatc ttctttcgat aaaaaatatt
     5761 tataaattgt tcctgtgctg attccgcttt cctttgagat gtctctaata ttaacggcat
     5821 gaaaatcttt tccggcaaac tgcctcaata caacaggata aagccgctgc ttcagtttct
     5881 gtggcattaa aggtttatcc attaccaccc ttccttatat atatgttttg aataccggca
     5941 aaatataaaa cattgtttca tttgtcaagt attttttaaa acattgtttt attttaatat
     6001 gataatgtta atatttgtcc aaaaaataca tttcctgctt gacgttgtct ttgcgaacaa
     6061 ataaatttat aaaaaactgg gacgctgctc atggcagaaa ataatatttc gcaaatcagg
     6121 atcaatggcc gtcttgttgg catagcggat ttggatcatg cgatagcaaa ggccgcccgg
     6181 ctcgacagca aggatgatat cgacattaaa aaatgtcttc ttaatgaaat atccgtgaac
     6241 aattatattc ctccttcagc acaaaacgct tatgccgatg cgctgttgag agaatttaag
     6301 attgcccacg ggttgcccgt tgccgaggaa ctatttgagg ggatgaatat cgaggtggtg
     6361 ggtatgggat gtgcacgctg caatcaattg gaaattgatg tgcgcgattt attgtccgag
     6421 atgaacatta ccgcaggcct caggcacgtc accgatttta aggaaattgc gcgatacaat
     6481 cttctcggct cccccgcttt ggtcattaat aataaggttg tttcagtcgg cgacgttcct
     6541 ccgaaaagca aaatccgtca atggattatc gaagcgcacc agcgcaccgg caaagattag
     6601 actaatcttt ctttatttct ggcaatcagg acaataaaaa gtgcttcgcc ccgcctgttt
     6661 gatccggcaa atctctcccc cgcattttga acagtgtgtc ccttcccgac cgtagacttt
     6721 caatgcatgc tgattgtcac ccgggcgacc ataaagatcc cgccaatctg aaatcgacgt
     6781 gcctctcttt tcaattccct tctttaaaat ttgccgagac gacgttataa ttttttccca
     6841 ttccccttct gacaaatccg acgccttcct gaaaggatga atgccggagg catataaaat
     6901 ttcagaggcg taaatattgc cgataccggc aaccgccttc ggatccagaa gcagcgtttt
     6961 gatagaaacg ctccgtttcg cctgtctgct cataaactcc ttttcatcaa aatctttcat
     7021 cagatcgttt tttattgtat ccttttttat atcgccgatc gttaccgtgg cgaaccgtcg
     7081 cgggtcaacc agatacagat accctttatc gaacgacaat cgccagcggg cataatccgg
     7141 tcgcagcgaa acctcccgcc agaacaatcg ccccgtcatg cgcaaatgga tatttatcgc
     7201 acggccatta tcgagctcta gaacaatggt cttgccctgc cgttgaacgt ttatcgcctt
     7261 tcctttttta aacgattgga cagcggacaa tttatcgtca tacactttac ctccggtaat
     7321 gctcctgccc gcaatctttt tctgaagctg ccggcagagc gtctctactt cgggtaattc
     7381 gggcatacct cgtctccttt ttgaacattc attactttca tatccggaca gaccaatgga
     7441 ttctgcgtcg caggaatatt tttttattcg ttttctgctg caccatgaca cagatcgccc
     7501 tgtcaggcaa cccctccctc ctgtgcgcat ggctgaacga tttttatgaa acacgccatt
     7561 tctgataatc gtctttgctg catgcgagct gatcgtggat gtttttataa attgcctatc
     7621 ctgcgctgag cctgatttga cggcctccag atcagtattc aacctttctc atcgttctgg
     7681 acatccaacc gcttttcgag ggcagttcct tcaaaacctg tcgccatcat gcggaagctt
     7741 ttcctctgaa cgtgtttcgt ctgaaaactt ttttaaaatt ttttttgaaa attgcaaaaa
     7801 aatgttgaca aatatttttg tatgagtaca tttcataatc aaagagattt ttattttaaa
     7861 atttttttta agggaggggc gtcctcatca aggaggcaca aatggaaagc gagaccctgt
     7921 ttaaagagga ggaagaacct cctggtcatt tgaccggtct tgttcatgtt aaaaacaatg
     7981 tcagtgactt tttgttattc aaagatgagg ttcttcctta tccggtaaaa caaaagtcag
     8041 agaatgtttt atttcctttt aaagattctg cccgctcccg cctcttccga tcacagtttt
     8101 atcccgagat tacccgtttt gaatggttcg actggcgctg gcaactgcgc aacaccatcc
     8161 gggatgccga caggctcgcg cagatcatcc atctctccga taacgaacac caggccatgg
     8221 cgcatcattc cggcgccctg cctgtttcca ttacgcctta ctatgccagt ctgctgaatc
     8281 ccgatgatcc tgccgacgct ctgcgccgat gtgttgttcc ggtggtggat gagtgcctgc
     8341 atacggaggg ggaagaggaa gatcctctct gtgaagacgc ggattcgccg gttccgggaa
     8401 tcattcaccg ttatccggat cgcgttttgt ttttagtgac cgacatctgc tccacctact
     8461 gccgctactg cacacgttcc cgcattatcg aacgcagcac gcatcatctc atcaaccttg
     8521 aaaaatggga aaacgccatt cagtatatcg ccggtaataa gaacatccgg gatgtgttgc
     8581 tctccggcgg cgacccctta accctgtccg acgacaagct tgaatggctg ctggcacgtc
     8641 ttcacaagat tcgccacgtc gaactgatca ggatcggcac caaagttccc gttgtgcttc
     8701 cgcaaaggat cactccccgt ctgacggcca tgctgaagaa ataccatccg ctatggatga
     8761 gcattcatat tacccatccc agagagctga cgccggagat gagcgctgcc tgcatccgtc
     8821 tggccgatgc cggtattccg ctcggaagcc agaccgttct gctctccggc atcaacgatg
     8881 atgttgccac catgaccaga ctggtacagg gtcaactgca aatcagagtc cgtccttatt
     8941 atctgtatca atgcgatccc atccccggct cttcccattt ccggactccg atcagcaaag
     9001 gcatcgagat catccagggc atgcgcggac ataccaccgg ctacgccgta ccgacttatg
     9061 ttgttgacgc acccggcggc ggcggcaaga ttcctttgct gcccgaatac gccatcgggt
     9121 gggaaaacgg ctccctgttg cttcgtaatt atgaaggcaa tatctttcgc tatcccgaca
     9181 accacgccaa atcgaaagat atgcactaaa caggcagtct tgacgacagg gggtataaaa
     9241 ttcaaccgtg agtcgcgtca gcaagaaact caaaatcggt ctcacctatg atatgcgcga
     9301 cgattacctg aaggaaggat acggtctcga agaaacggct gaattcgatc ttcccgaaac
     9361 catcgaagcc attgaacgcg tcattctgga caacggtttt caggccgacc ggatcggcaa
     9421 tattaaagct ctgtcccgaa ggcttgttgc gggagaccgc tgggatctgg ttttcaacat
     9481 tgccgaaggc atgtacggtt tcggccggga agcacaggtc cccgctcttt tggaagccta
     9541 caacatcccc tacacctttt ccgatcctct gggcaacgcc atcacgctgc ataagggctt
     9601 taccaaacag attctccgtg atgccggcat tcccactccc gcttttgtcg tggtcgacac
     9661 agccgctcac atcgacacaa tctcccttcc ctttccgctt tttgccaaac cggtggcgga
     9721 aggaacggga aagggcatca cagccctatc caaaatcacc agccgaaaaa acctgcgtgc
     9781 cgtctgcctg aatcttttaa agactttccg gcagcctgtg ctggtggaaa cgtatctgcc
     9841 gggacgcgaa ttcacagtcg gtatcctggg aacaggcaaa gacgcccgcg ccttgggcgt
     9901 tctggaagtt attttaaaac cggaagccga aaaaaacgcc tactcctacg aaaacaaaga
     9961 acactacgac aaactggttg actatgtcct ggtcgatgat ccagaggccc gaaaggctgc
    10021 ggacatttca ttaatggcct ggagggcatt ggatttaaga gatgccggtc gtgttgacct
    10081 tcgctccaac acgtacgatg ttccccattt tatggaagtc aattcgctgg ccggtctcaa
    10141 cccggtgcgt tcggatttgc ccatcctgtg ttctaaaatt ggcatgagct atcatgagct
    10201 gatctgcgcc atcattaaat cggcgctcca acgtgtaaaa aatcatcctg aaaaccggtg
    10261 taaataattt ttatgaaaaa agtagttatt ctgcacagtg atatcgcaac ggatggcagc
    10321 ctggacgaac tggattgtct cgtgcaggcc gaagccattg ccgacgccgt gcgcacgctg
    10381 cattatgaac cggttttgct gccttttgaa ctcaacctga atcgtaccat aaccatgctg
    10441 caatcattga aaccggcggt cgtttttaat attgtagaaa ccctggactc caaaggcagc
    10501 ctgattcatt tcgctcccgc tgttcttgat gtgttgcaga ttccttatac cggttgcccg
    10561 acacacgcca tatacctcac gtccaacaag ccgacggcca aaaaaatcat gcgcgatgcc
    10621 ggcattgcca cgccggattg gctggaacag aatggcatca tcttccgaca ggatgatggc
    10681 ggaaaatttt taatcaagtc gtcctgggaa catgcctcga tcggtcttga tgaaaactca
    10741 ctgatttcct atacggacaa aactgcgatc atcaaagaaa tgaaactccg cgaaaaaaaa
    10801 ttgggcggct cttgttacgc cgaagcctac atcgacggcc gcgagttcaa cgttgcttta
    10861 atgtctgatc aggccggggc cagagttttg ccgatagcgg aaatgctgtt tcaggattat
    10921 gctccggata aattgaaaat tgttgattac aaggccaaat gggacgcgga ttcctttgaa
    10981 tacaataata cagtgcgaaa attcaatttt gatgacgagg atgccctcct catatcgtcc
    11041 ttacaggaca ttgccctgca gtgctggaat attttttcgt tgcgcgggta cgcgcgtgtt
    11101 gactttcgtg ttgacaagaa caacaagccc tgggtattgg aggtcaacac caatccgtgt
    11161 ttgtcgccgg atgccggttt tgctgcggcg ctggagaggg ctgaaattaa atatcacgag
    11221 gctatcggtt taattattga taacgcgttg aaatctatat ccaaggagat ctaatatctg
    11281 tgttaaaaac catcatattc cttttctttt ccaacttatt catgacgttt gcctggtacg
    11341 gacatctgaa aaacttcaac cacaagcctt tattgtttgt catcttgttc agttggggcg
    11401 tcgccttttt tgaatatgtt ttacaggttc ccgccaatcg tatcggtttt cattattaca
    11461 gcctgggaca gcttaaagtg attcaggaag tgattaccat gattgtattt gccggttttg
    11521 cgatcatgta tatgaaacaa ccgttgaaga tggattttct gtgggccggc ctttgcctag
    11581 tcggagcggt ttacttcatg tttcgtgaag gcctcccgca tacgtgatgc aaaatatgaa
    11641 aaaatttatc tatcgccagc aaattaaacc ttccgacatc gaagtcattg ccgatatggt
    11701 ggaatccagc ggcttctttt ctgatgaaga aatcgaaata gccatcgaac tggctgaaga
    11761 taaactggac aaacaggagg tcagttctta ccagttttta tttgccgaaa ttgaaaacca
    11821 ggtcgtcggc tatacgtgct acggcctcat accggccacg gccgggagct acgatcttta
    11881 ctggatcgtc gttgccaaag atttacaggg gctgggagtc ggaaaaatgc tgatggccga
    11941 aacagaaaac cgcatttatc aaaacggcgg caggcaaata tacgcggaga cctcctcgcg
    12001 ggcacaatac acgcccaccc ataaatttta tgagtcctgc ggttatgcca aggaagcttt
    12061 tctgaagaat ttttactcgg aaggggacgg aaaaattatt tacgctaagg tgcttaaata
    12121 aaaggattta attcctttct tctttgttct ggcggtttta attcttgcat ttttcctgat
    12181 aattcgctag agcactgcca gtaatttgct gacgttccgt cagcacggat tacaccagga
    12241 acgcaaggag gtttatctca tgcgcttgtt ccccaaagaa gaaaattttt ttgaactttt
    12301 tgatgaactt gccgataaaa ttgaggaagg cggcaagctg tttctggaaa tggctcagac
    12361 ccgggattat tccgatgtgc gggtcgccaa actcaaggaa attgagcacg aagccgacgg
    12421 catcacccat aaaacatacg aaaaaatgca caagacattc cttacgccta ttgaccggga
    12481 agatatttac gcactggtca ataaaatgga cagcattatg gatgtcattg aagcaacagc
    12541 cgtccggatt catctgtata aagtcaaacg aacgtcggat gaaatcatca aacaggcaca
    12601 aatcctgaat gatgcgatca aaaagctgaa aaccattatt caggccatgc ggaacatgaa
    12661 aaactccgac atgattcttg ccggttgcgt ggaaataaat acccttgaaa atgccggaga
    12721 tatcacgcta aggtccatta tggccaatct tttcgaaacc gaaaaagacg ccattgagct
    12781 tgtaaaatgg aaagatattt ttcaactcct tgaagaagca attgatgtct gcgaagacgt
    12841 atccaacatc gtggagggga tcgtcctgaa acatgcttga ttcaatggcg tttgttgttt
    12901 ttctcatcat tggtgcgctt gcctttgatt ttctaaatgg ttttcatgat tctgccaatt
    12961 ccatatccac tgtcgtttct acccgcgttc tctcacccaa atacgccgtc atctgggccg
    13021 cctttttcaa ctttgccgcc gtcttctttg tgggaacagc tgttgcccac acggtaggta
    13081 aaggaattat taatattgac attgttgata atctggtcat ccttgccgcg ttaggcggag
    13141 cgattatctg gaacatcatt acatggtact atggtttacc cagcagttcg tctcatgcac
    13201 tcatcggcgg attaatcgga gccgccatct cgaaagctgg cttcagcacc ctggtatggg
    13261 caggcatcat caaaacgact gttttcatca tcgtatctcc ggccatcgga atggcgctgg
    13321 gttttatttt tatggtcacg gccatgaatt tgagccacaa ctccaacatc ggcaaatccg
    13381 acaaactatt ccggaaactt caacttttat ctgccgcggt gtattcgttg ggacacggta
    13441 tgaacgatgc gcaaaaaacc atgggaatca tcgccatggc actgttcagc aagggattgc
    13501 tgggtgatac gttttacatt cctgtttggg tcattattgc ctgctattcc atgatttcac
    13561 tgggaaccat gttcggcgga tggcgaatcg tgaaaacgat gggaacaaaa ataaccaagc
    13621 ttcagccgat gggcggattc agtgcggaga cggcggcggc ctgctcgatt atcggcgcta
    13681 cggttgccgg tattcccgta agtacaaccc ataccattac cggcgcgatc gtcggcgtcg
    13741 ggtccacgag aagactttcc gctgtgcgtt ggggtgttgc cggaaaaatt atctgggcct
    13801 ggatactgac cattcccgct gccgcgattc tctcctcgat catctatctt tgtgtccaac
    13861 tctttatcac ataacagata tgtcccgttt caaaatagta ttgataattt tccggttttg
    13921 ccgtaaattt aacaacacgc ttgatttcac gtaaattatt tttatccagg aagactgtta
    13981 tgcctcaaaa gattttacct ttttcgaaaa agcaaatcga aaatatcatt aaagattatc
    14041 cgacgccctt tcatatttat gacgaaaaag ccatccggga caatgcccgc gctttcataa
    14101 acgccttcaa ctggaatgaa ggatttaaag aattttttgc cgttaaggct gctcccaatc
    14161 cctatttgat gaaaatcctt cacaaggaag gttatggcgc cgattgcagt tcgctggccg
    14221 aattgatgat ggcacagaaa accggcatta ccggcgaaga aatcatgttc tcctcaaacg
    14281 ataccccagt ggaagatttt ctgctggcca aaaaattaaa ggccatcatc aatctggacg
    14341 atatcagcca cattgccttt ttggaaaaaa gtgcgggctt gcctgcactg atttgtttcc
    14401 gttacaatcc cggcaaactg aaaaaaggca atcatattat cggccacccg gaagaagcca
    14461 aatacggttt cacccgcgaa cagctttttg aaggctataa aatatgcagg gacaagggcg
    14521 ttcggcgttt cggcattcat acgatggtcg cttcaaacga acttgaccct tattattttg
    14581 tggaaaccgc ggaaatactg ttcaacctga ttgttgagct ttcacagaag ctgaaaatca
    14641 aattcgaatt cgtcaatatc ggcggtggag taggaatacc ctaccggccg gagcaaaaaa
    14701 gcatcgacgt aaacgtaatg agtcgcggca tcaggaaaaa atacaatgat attatcgtgg
    14761 ccaacggtct ggctccttta aaactattta cggaatgcgg ccgctacatc accggaccct
    14821 acggttatct tgtttcccgc gtgcggcata tcaaaaacac ctataaaaaa tttgccggac
    14881 tggattcctg catggccaac ctgatgcgcc cggcgcttta cggcgcctat catcacatca
    14941 ccgttctggg caaggagcgc aaatcacaca atatccttta cgacgtcacc ggatcgcttt
    15001 gtgaaaacaa cgacaaattc gccattgacc gcaagctgcc caaactggag cagggcgata
    15061 ttctcgtcat ccatgatgcc ggcgcgcacg gttttgccat gggcttcaac tataacggga
    15121 aacttcgttc agccgaactg ctactgagag caaatggaaa agtggtccaa atccgccggg
    15181 ctgaaaccat aaaggattat ttcgcaacgc tggattttaa cggactgaaa gatttcaacg
    15241 cgtagatata aaaggaatga cgcagatcag tgtttcactg atctgaacgt gcgtcaaata
    15301 aatcgacttc cagacggatt gagacagagg catgcggaca ttaatgatgt taccgtcatg
    15361 tcttctttaa aattttctac aaccatcaca aaacccttta gatattatgg cttcattcaa
    15421 agatattttt aacgcagctg aacaagggaa ccccgccgca cagtataatc tggggttaat
    15481 ttaccggaag ggcgaagccg tcccgcagga tgacgctgaa gccgtcggct ggtttctcaa
    15541 ggccgcccag caaaatcatg ccaaagccca gctgaacctt gcctggatgt atcaggaagg
    15601 aaaaggagta tcacaggacg atgtaaaggc tgccaagtgg tatctgaaag cggctgagca
    15661 gggtaatgca caggctcaat tcaatctggc cctcatgtac agcaagggag aaggtgttgc
    15721 actggatcat attgaagcgg caaaatggtt tttggaatcg gcggaacagg gtatcgccaa
    15781 tgcgcaatat aatctgggca tcatgtacca ggacgggtta ggtgtccaaa aagattctac
    15841 cgaagctgtc aaatggttca ataaggcagc caagcaggga cacacggatg cccaggctaa
    15901 actcgatgcc atgcgcaaag ccctgcagga cgagcagcgg gaagcagccc gggcggtcaa
    15961 ggaatcccct cgcatccttc catcaaacgc cgccgatgct tcatccatac cggacgagtt
    16021 tcccgacgag aagaaagaag ccctgtcaca ggaccacgcc aaacaaatcc ttgtaagtca
    16081 atatctgaag gcggctgaac agggccttgc cgatgcacaa tatcatctgg gcgttatata
    16141 cgaagaaggt tccggcgttc cccgggataa tgaaaaagct ataaaatggt atcgcgccgc
    16201 ggccgaacag gggcatccag aggcccaaaa taaactggag gctctgtctc cggccggtca
    16261 cgaaaccatg ccaggcgata cacagccccc cgttgacgga aacgaaacca ctgcatatac
    16321 aaaaccggat gaaagcgcga acgcgcttat agttgtaact gatgagcttg ctgaacaaaa
    16381 tcatgatatt gagcaggagg ttccggaaac aacagcgacg gaaagcaatc cctcgccgga
    16441 gatttctgat gacgaaaaag ggtatcgcga agcggccgaa cagggcgatg ccgatattca
    16501 aaaccggctg ggcttaatgt ataaaaacgg cgacggcgtt atgcagaacc acgttgaagc
    16561 ggcaaaatgg tttttaaagg cctcggaaca aggcttcgcc gatgcgcagc ttaacctcgg
    16621 caacatgtac caggaggggt caggtgtccc gcaaaacgat gctgaagcgg caaagtggta
    16681 tcgcgaagcg gccgaaaaag ggcatgccgg tgcacagtac aatctgggat taaaatattt
    16741 tttcggggaa ggtgttcccc gggactttgc tgaagcaatc aaatggtacc aaaaagcagc
    16801 gcttcaggac aatgtcgatg cacaattcac cctgggcggc atatacgaag agggtgttgt
    16861 tattccggaa gatcctgctg aggctgaacg gtggtatcgc cgggcggcgg aaaagggcca
    16921 tgagcgggct cggtatcgtc tggaattgat gcacaggcac gtctgatcga ttcagcacaa
    16981 gccaaccgca acggatttta agaaaaaatc ctgtcttgat ccaccgcgcc aaaacaccgc
    17041 ttgattattt gtcagacgaa ccgggccgtt ttcagaccaa taccaaacat ccgcctgccg
    17101 ttgcgcgttt tttattcacc cggcaagcac tgaaagtata aagatttttt tacgctctat
    17161 aaacttcatt ttcatcctgg aatccccaac gaataattca ttgacacggc aggctgatta
    17221 tcatatgaac tctcacatca ataacaagga caatgggtta cggtgatttt tcttttaaat
    17281 aattttcaaa aaataactgc cttctcgggt tctatgttga aaatctcgcg acacatccat
    17341 gaaaaaagat ttcctcagat tctcacactt ttttttacgc tgatccttgt atgtctgacg
    17401 atggtctggt cggccgatcc ggcttattcc aacgatacac aggacaaact ttattctctc
    17461 aacctctact atggcgaaat atcgaaaaat catgccgatg atttcatttt ggacaccgga
    17521 acactccggc tgaccgattg ccgcctggca gccgtcacgc tggcacgccg tctctatgca
    17581 tacaaagatc tcgccaatct ggaagttgag ggacagattg taaagcattt ctacgacatc
    17641 caggaccact gggaatttaa cgccattgta acggctcgct ggaatgcctt tttttgggac
    17701 aagtatgtgg atacaagcat cgcgttcggc ctgggcccct cttatgcaac agagaaaccg
    17761 gaaatggaag cgattcttga ccgtgagaca tcccaatggc tggcgtattg gatggtcgaa
    17821 ctggaaatgg gtctgcccgc ccatcccggc atctccttca ttacgcgcct tcatcaccgc
    17881 tcggatgcct ttcgcattgt atccccgggc ggcgcctcca cagcacccgt tgtgggattg
    17941 aaaatccggt tctgacaatt cgctgttaaa atccgcatcg atgtcttccc gacggggaag
    18001 cggccttgtt tacaaaaaat atcaacaaca aaccttgcta tgttttgatg agttatgata
    18061 agccatttcc agcggcaaac atccgcaatg atgagatttc tttcgagaag atataagaaa
    18121 aggggcccgt tgtttttttt tgcggcccgg tgtaaaaaac aattaagata aaagttccgt
    18181 aaaaacatta ccatgaacac aaaagcaaat cgtgccatag gcgttttcga ttccggcatc
    18241 ggcggtctga cggtcgtccg ggccctgatg gaacgtctgc cttttgaaaa catcatctat
    18301 ttcggcgaca ccgctcgcgt gccgtatggt gtgaagtcgg tggaaaccat caaccgctac
    18361 gcggcacaga ttaccgaatt tctgatccaa aaagatgtca aaatgctgat tgtcgcctgc
    18421 aacaccatgg ccgcagtggc tcatcaaacg atcattgatt tatcacaggt gcctgtcgcg
    18481 ttcgcttatc aaaaacttgt ggaacaggta cccgtcctcg aagtcattga cgccagcgcc
    18541 cgcatggcca tccagcatac caaaaccaag tccatcggcg tcatcggcac acctgcaacc
    18601 gtcaacagca atgcctacgc cagggcaatc catcttctgg ataaagaggc aaaagttttc
    18661 tctcatgcct gtcctttgtt cgttcccctg gtggaggaag gatggtttga tcatccggcg
    18721 acaaaactca ttgctcagga atatctcaaa caggttatcg ccgaacaggt ggacacgctg
    18781 gttctcggct gcacgcatta cccgctttta aaacccctgt tacaggacat tatgggaaaa
    18841 tccgtaacac tgatcgattc ggcagaagcc atggccggta tcgccgccga tctgatcgat
    18901 caacaagcga tcggcaatca gaaccatgcg gtaccggaat acacgtttta tgtcagcgat
    18961 ttgccctatc gttttcaaac catcggcgaa cgttttctgg gaagaacgct gggaaagatt
    19021 gaacttgtca gtttttagga aaatttcatg aaaattcttc ttgccggtca taatctggac
    19081 tatgacatca tccggacgct gaaagacaaa agcggaacaa aacaggattt gacacctgaa
    19141 accatctcgg cggcttacgc acgcatcagc cgcaatccga aacccgttaa cgagctgcgt
    19201 gccatcgccc gcgaggaagt ggataaggct cgccaatcca accgtaaaat cgtttttgac
    19261 atggggcaca actccgtggc cgagcatgcg gtttttaata tcgatgtgat cggcgtctcc
    19321 cggttcctgg ttgaagaaat cgaaaaattc cgtctgtgtt ccttcacgga aaaatcacag
    19381 cgctatgttc ttttcgataa ggattttgtc gtcccggaag aaatccggca ggctgatctt
    19441 gccggtttgt tcatctctac cgttgaagcg caaaatgatt tttaccatga gatgtatgag
    19501 aacttgaggc cgtacatttt cgataaacat aaaactctgg cgtccgatcc ggcgaaccga
    19561 tccatgctgg aaggctgggc caaggaagac gcccgctacg cgatttccat ggctaccgaa
    19621 acccaactgg gcatgaccgt caacgcccgc aacctggaac tcatgctcag acggctggcg
    19681 gcattgcccc tggccgaagc gcaggaatac agcgcaaagc tgtatgccgc cacaaaggaa
    19741 atcgcccctt ctctgatccg ttacacgaag gcgacagact acgataaatt cacaagaaga
    19801 aatctgaatc ggttttccga cgcattaata aaaaaatcgc aatcgaaggt caaaaacgca
    19861 gccgatgtgc aattgatgtc tgcatcggct gctgccgata accgggcggc tgccgcattg
    19921 ctgtttgcat catcggatgc caattatgcc gaatgcctga agacggccga caagctgacc
    19981 gctcagaaaa aaagatccct gtttaaaact gcttttgcca acctgcagat acacgatgcc
    20041 gtattaaggg agctggaaac tgtggatctg cagtttgaac tgatcgccag ttccagttgc
    20101 tttgcccagc ttaaacgaca ccgaatgtcc actatcattg cacaggaata caatccgcat
    20161 ctgggtgtca ccgtgccgcc ttccattaaa gccatcggcc ggcaaaaaga ttttctggac
    20221 ctgatgcacc gtacgcagag cacatatgaa aaaatcagag agaaagcacc actggctgcc
    20281 gtttatgtgc tgaccaatgc ccaccgtaaa agggttttga tgaaactgaa cgcccgggaa
    20341 atgtatcatc ttgcacgact gcgtgccgat gcccatgccc agtgggacat tcacaatctt
    20401 acgggaaaaa tgctcaaaca ggcgaaaaaa gtgatgcctc tgacgctgat gatggcctgc
    20461 gggaaggatc atttcccgtc gctcttgaac aaaacgtata gctgcacgta attgtccgta
    20521 tttatcatcc atccatgaaa atcaacgcca ataaaaatca tctctatgtg ctgctggtta
    20581 caacgctgag ttcttttctg acaccgttta tgggatccgc catcaacgtt gccctccccg
    20641 ccatcgccga agaattgtcc atgaccgctc tgacgctcag ttgggtggcc acttctttta
    20701 ttctggcggc atccatatcg ctcatccctc tgggacgtct ggccgatatc tacggccgcc
    20761 gaagaatttt tatctacggg gctttgattt ttaccgtggc ttccgccttt tgcatctggt
    20821 cgcccacgca ggcttttctc attgccgtcc gagctttgca gggcatcggc ggggccatga
    20881 ttttcagcac cggaacggcc atgctgattt cagcttatcc gccaagcgat cgcggcaaga
    20941 ttctgggcat caacatcacc gccgtttatg ttggtttgac tgtcgggcct tttatcggcg
    21001 ggctgctgac gcaatatctc ggatggcggt acatttttct gtttaccttc ttcctgggct
    21061 taaccatcat tttcatcacg ctggcaaaaa ttgaaccgga aacatccgcc cgcgaagacg
    21121 agggcttcga tttaaccggc gcgctgatct acgccattgc tcttttttcc attatgtacg
    21181 ggttttccca tctgccggca tttagcgcgg ggtgtctcat tgcgatcggt atgatctttc
    21241 tgggcttatt catttttcag cagctcaaaa gccccttccc cttgctggac atacgcctgt
    21301 ttctcgataa caaggttttt gctttctcca atctggccgc cctgatcaat tacagcgcca
    21361 cgtttgttat taatttcctg ctgagtcttt atctgcaaaa catcaaagtt ctgacccctt
    21421 ccgaagcagg ctccattctg atcgccgccc cgatcatgca ggcgcttatt tcgccttttg
    21481 ccggacgtct ttccgaccgt tttgaaccgc aaatcatcgc gtccatcggg atgtccatca
    21541 cggtcgcggg tcttgtcctt ttaattttcc tcaacggttc cacgggcatt cattatgttc
    21601 tgttttgtct tgttttactg ggcatcgggt tcgcgctctt ttcgtcgccc aatgtcaatg
    21661 ccaccatgag ttctgtggaa aataaatttt acggtatcgc atcggccaca ctgggtacga
    21721 tgaggcttac cggtcaaatg ttcagcatgg gcattacgat gctgattttt gccgttattc
    21781 tgggcaacca tccgatttcc gccgccaatc atcatctctt tttaaaaagc accaaactta
    21841 ttttttccat cttgagtatt ctttgctgcg cggggatctt tgcttcactg gccagaggga
    21901 aaatgcatga aggcaatcac tctctctagt tgtattatcg gactggtcgc cggcacgttt
    21961 ggcggtctga tcggcatcgg cggcggtatc attatggtgc cgctgatggt cgagatatta
    22021 aagctgcccc agcacaaggc tcacggcaca agtcttgtgg ccctggtttt cacaggcatc
    22081 agcggatcaa taacctatgc actgaacgga tcgatagaca tcaaagcggc ggcactgctg
    22141 gcgcttacgg caatcatgac ggcatccctg ggcgctcgtt acgccggttc ccttacggaa
    22201 caaaaattaa aaaaagcttt cggggccttt ctgcttttct gtgcagcact cttattttta
    22261 aaactatact tgccgaatgt cgtcgtaagc gatgatgcaa aaccggttat ttatctgaca
    22321 acaggagcgt tgaccggatt tctttccggc atgatgggcg tcggcggcgg cacgattatg
    22381 attcctgtga tggtaatact tgccggcttt acccagcatg tggcacaggg aacatcgctg
    22441 ctggtcatga ttccctcggg cgctgtcggc gcactcacac actggaaaat gggcaatact
    22501 gaacaaggaa tcctctgggg gcttattccc gggattcttt taggcacata catcggagga
    22561 actgtggcta atatcatccc gaatgatacc ctgcgcctga ttttcgccgg catgctgatt
    22621 tttctaggca ttcgttattt tgggatgtct tccccccgaa gaataggcaa gggttgatta
    22681 tgaaaaaaac agatggctat cgtaaatttt catttttcac cgacgttcgc ggaatgaaaa
    22741 atgcgatcgg tgaaatgcgt ggatggggcc atcctcatca gccaatattt aatgccggtt
    22801 atcagcgctt gcctgatgat cccgtattca atgaagattg gtttatcagt accatcaaag
    22861 aaaagatagc cactcatccg gaaaatacca tggaatatcc cttttacgga gagaaaatct
    22921 tcacggagcc gctgatagga tttattcagg gtgacgatcc gatatttcaa cagtataaaa
    22981 cgattatcgg accgcatcac ttcacgccgg aagaaattat gcagtggcag gccaggcaac
    23041 atggcgttcc cgctccccgg gcagaggatt taagcgtcgt atctttcatc ctgccgctga
    23101 cgaaaattac aaagaacgat aatgcggcca tgaaagaatg gtccgcggaa cgttgggcgc
    23161 agacgcgcct cttaggtgaa atattcagcc agatattcgt gcgtgaaata gttacctgtc
    23221 ttatgaaaag gggcattttg gccatagcgc cggacgtcac gcccatgttt aataaaaaac
    23281 gttatcccaa agtaggctgg gcttctccct ggtcgcaccg gcatatggcg tatgctgccg
    23341 gtctgggaac cttcgggatg cacgattttt tcatcactga aaaaggctgc gctcacagaa
    23401 cgggcagttt tgtcgtcaat gtaaaactta catcaaaccg tgagcgacct gatgatattc
    23461 atgctcattg tctgcaatat caaggcaaat cctgcatgca atgcgcgaaa cgctgtccgg
    23521 tgaatgcgat aaccgcggaa aatggccata ataaggaggt ttgctatcaa agggtggcca
    23581 aatctctgat acactgcaat aaaaattatc acatttttat ctatgggtgc ggtctttgcg
    23641 caaccggagt cccctgtgaa tccgaaatcc cgaaaatcct gcaaaaaaat tcaaaatgaa
    23701 aataacaaaa aaaccggggt ttattccggc ttttttgtta tttgttttta aagtttcagc
    23761 tttttaggag ctgttcaaaa aagcttagac acaaggcacg tgcccggcat tcgccgggtt
    23821 tccctttatt ttaaaatggg tgctacgcga gcatctttgt ctggacgcct ttcaaaatat
    23881 cttccgtctc cttcatcatg gattgcacca tatccgcgac tttgggcatg tcgttgatgc
    23941 gttgcgctgc ttcgcctgct gccagaagcg ccttctcttt gttgccgtcg taagcagcct
    24001 gcatcgactc catttcaaac tcaattaatt tgaagtcgat cgacagcata tcatccggcg
    24061 ttcccagata acatccgggc gatttctgca gtgtgttttg ggcgtgctct tcactgcgcg
    24121 gatttttaag ccatcgcgcc ggcccgacaa agccgcgggc aaccagagtg cctctgtcac
    24181 ctgcctcgac cacaccttgt cgccagatcg ggggaaaatc gctttcttgt gttgcaagaa
    24241 aacgcgtgcc catctggacg ccgtcggcgc ccatcaccag ggccgcggcc agcgtcttgc
    24301 cgtcgcagaa accgccggct ccgcatacca aagttttctc atcggaaacc gcttctacaa
    24361 cagcaggcag cagaatcatg gaatgcaccg gttcccagga tgtatgaaat cctccctcgt
    24421 gtccggacgc cacgatgaca tcaacccctg cttttttgca gcgaagagcc ccttttactg
    24481 aaggcaccac atgcatccag gtaaaaccgg cacctttgat tttttctttc catggcaccg
    24541 gatcgcccgc agacgtgaaa ataaccttga aatgtttttc catgcccgga ttggctgccc
    24601 gcacctgaat ggccgcattg atgataagct ccgcctgaaa aagcaattcg gcggagacca
    24661 tgacgttgat accgaatacc ccgtttgtgt ctttagtcaa ccggtaggct cttttgagca
    24721 atttctcaat tgcggtggga atgtcgtcat ccgggttggc ctcggcgctc ttaatccagt
    24781 cattgtaaat aaagggcaga aaatccttat tgcagagtcc gctcgtggaa aacaagccca
    24841 gcactccggc gttggcggcg gctacgccga gattgttgtt actgaacggt cccatgcccg
    24901 cctgaatgat gggatatttg attccggtca tgtcgcaaag tcttgatttg atcatataag
    24961 acatctccta taaaattatt gtatcttata cacatcactc tctgatgcgc tttttgagtt
    25021 ctcgaatcat atgattgagc acgtcgcttg catcaccgat gatggcgaca tcggccattt
    25081 tcagcatggt tgcctgcgga tttttattga tcgcaatgat gaaatgtgcc tcgcccaagc
    25141 ctgcggtgtg ctggatcgca ccgctgatac caaaggaaaa caggagtttc ggcttgatat
    25201 gctttccggc ctgacccacc agtgcgtcgt ggtccgccca tcccgcgtaa accaccggac
    25261 gggtggcgcc gacatcgccg cctaaaagtc tggccagttc ctgaagtttt ttaaattttt
    25321 ccttattgcc catgccgcgg ccgccgcaaa taataaccga cgcgttttcg atggtatgct
    25381 ctttttcaac ggaacgttca tattcgataa gacgaacgcg gggtttgggg agatttttca
    25441 tcaaaggaag gcgttcaacc aacgctttcc gctggttatc gtgaggaatt tccttaaagg
    25501 tgcccggacg aaccgtagcc atctgcggac gatggcgctc cgtgacgatt tccgccagca
    25561 tattgccccc gaaagaaggc gcgatctgca gtagcagtcc gtcatcggcg atatccagcc
    25621 cgacgcagtc agcagataat cccgttttca gtcgtttggc cagccggggc gcaaattcac
    25681 gtccgaaatc cgttgcgccg atcagaataa tttccggatc tctttctccg gccaatcgtt
    25741 caatgatggc cagatatttt tcggtgctgt aacttttcag cgccggatca tcaatcgcca
    25801 aaatacggtc cgcgccatgc gcagtatatt ccataatcca ttcgtctatt tcatgaccga
    25861 aaatcaatgc gcatacttcc gcgtcgatga ccttggccaa cgcaaccgcc cgggcgatca
    25921 attgcagggt gacccggttt tgataataat tgcggtaatc gccgaatacc cagatacttt
    25981 ttttactatt ttttttcatc ggacttcttt tcttcaatgg atttccctat gacagtgccg
    26041 atttttcttt gatacttgtc caaaaactcc gtcaccaccg cagacgccgc tccctgcaga
    26101 agcacgttcc ctttctgcgt cttcctcaga aacaccttgc ggactttggt gcgggagcct
    26161 tttttttcaa tggatgcagc cgtcaagccc agagctttcg catccaggat gacaacatca
    26221 ttcttgtcga aggcgctttc cagacccgcc aaccccgtat aacgcggttt gtaattttcc
    26281 atcgagatgg tcaccagcgc cggaaattcc atttccagtg tttcgcggaa attatccacc
    26341 agacgccgga cgcgtaatgt ttttccggaa atttccagtt tttccacata cgccgccgcg
    26401 ggtaaatcaa gctcctcggc cagttgcggt ccgacctggg ccgtttcgct atcggccgtg
    26461 taggctccgc aaaggatcaa atcaaatttc ggacatttct ttttgatcgc ctcttttaaa
    26521 ataagggacg tggcaaacgt atccgatccg gccaacagcc tgtcgcaaac cagcacggct
    26581 tgatcggcgc ccaacgccag cgattcccga agcacttcct cggcggccgg cggtcccatc
    26641 gtgattacgg tgatattggc gggatggtgt tccttgattt gcagagccgc ttccagggcg
    26701 tgtttacagg ccggattcat catgccggcg gcaaggtcgc gccgcagcgt tcccgtcttt
    26761 tcatcccacg gcagttcggt taccatggga acctgtttta aacaaacgac taaattgagc
    26821 atgtttataa tatcaactga gacagtatgg atcgcgcgat aaccatgcgc tgaatttcgc
    26881 tggtaccttc gtaaatttcc gtaaccttcg catcgcggaa atacctttcg acatcgtatt
    26941 ccttgctgta tccgtaaccg ccgtgaatct gcacagccag agacgtcacc ctggatgcga
    27001 ccgtactgca atacaacttt gccatggacg cttcacagcc aaaaggcatt ccctcatcct
    27061 tcaacgagca ggcgcgatac aaaagcagac gcgcagcggc gatttcggtg gccatatcgg
    27121 caatataatt ctggatggtt tgcagcgaag cgatgggttt tttaaactgc tgtctttctt
    27181 tggcatattt aaccgccgct tcaaaagcgc cctgagcgat ccccagcgcc tgcgcggcaa
    27241 cgccgatgcg gccgttatcc agcgtagcca aacctatttt catgccgtcg ccgatcctcc
    27301 ccagcacatt gttctccggc acacagcaat cttcaagaaa aagcgacgaa acaggattgg
    27361 cgcgcatacc gcagagatct tccagttcgc cgcgtaaaaa tccgggatat ttgctttcga
    27421 cgataaaaac gctgctttgc ggcttgccct gttcagagcc ggtcagcgca aaaatcaaag
    27481 ccagatcgca gacgccgccg ttggtcacaa atattttcgt cccgttcaaa acgtacgcat
    27541 catttctttt gaccgccgtc gtttccacgc ttgcggaatc ggacccggcg ttggcttccg
    27601 tcagactgaa tgcgccgatc aactcgcctt cggccagttt gggcagatag tgtttcttct
    27661 gctctgcgtt cgcaaaccgt aaaaagggat atacggcaac accattatgc accgtcacgc
    27721 acaaaccgat cgcggcgcaa acacgggaga tttcttccac aacaatagcg gcgctgacgg
    27781 aatccagggc ggctccgccg aattcccgcg gcacctgcag gccgaaataa ttcaacggcc
    27841 tcattttctc gatgatttca cggggaaacg ttgcagcgcg gtctattgcg gacgcaacag
    27901 gcgcaagttc cgtctccgca aacgtccgca cggaatcgcg aatcagttta tgcttggcgc
    27961 agggattcag ctgcaatgtt ctctcctgga agaaaatgct ttgcacaaat aacttaaaat
    28021 aaccgtaaaa tcaagaaaac cttattccga aggtctttgc ccgtttccgg gaaccggtat
    28081 taatcctcag ggttatcaaa cgcatgatat gtcattgaca gccgctacgg ttgactctat
    28141 acttctatgc tatggtacac cgcgaggcca aaaataaatc aagccggaag accaaagcag
    28201 tccatcatga cccggacgct cttccctcgt taatggccaa tcctcaggaa aaactcgctg
    28261 tttcgctccc tttacggctt atcatccgac tggctttagg atttgtcgat ctttttcata
    28321 tttggcgttt tccccgcaag accgacggtt gcttcgaaca catgaccctc atcgataagt
    28381 tgcactggct ttacaaatcg gcaaacccga tccgtttagc cgaaaaaaaa tcttctcttg
    28441 aggacttttt cgccggacag gcggaaacca gccttacgct gccgccggat tttgaacaaa
    28501 ccactcaaat acaactggct gccgtgggcg atctgatgca gaacgatctg ctgacgcatt
    28561 cggcggattc gctctatgaa ggcattgccg ccgaattatt tactgcggat atctcctttg
    28621 ccaatctgga agctcccttg accactcagc cgcaacgcaa tttattattc gtccgcagtg
    28681 agaccccgtc aacctacatt tcggccgggc aatttcaggt gctcaaagga tacaaagatc
    28741 agaaattcac cgtcttctca actgccggca accatgcctg ggactatggc gaggagggcg
    28801 tgctcagcac cctcgacgct ctggcaaaag aagggattgt cgccgttgga acgaatcgtg
    28861 aaaaagcgga tcgacagaag gcggcgatca ttgatgtcca aggcatcaga ataggcttta
    28921 tcggattaac cttcggcctc aacggcaaga aaatcgatcc ggaacaggaa tatcatgtca
    28981 atttggttca actgaatgct ttatctgcgg aggtcgatct gtctgtgccc atccggcaaa
    29041 tcaactactg ccgggaacag tactgcgatt ttatcgtcgg gtccctccac tggggtcttg
    29101 aatttgaatt ttttcccgcc cgacgccagt tggccgttgc tcatcaactg gccgaagcgg
    29161 ggctcgatct gatcatctct caccatccgc atgttgtcca acctgttgag ttttaccgga
    29221 cccggcgcga tccgcaacgg acggtggtca ttacctattc cctcggtaac atggtcagtc
    29281 ctttccacga cgacgacctt aacctgagtt ttcttctgca ggtgaccctc gccgacggga
    29341 aattgaaaaa tcaaaagcga acctatattg ccgcctgccg cattattccc gtctgtcaga
    29401 gagcattgat ggccggcgga aagccgacgg tgcggctgga aaaggtaacc cgtttggccg
    29461 cattccggct gccgtttgac ctcaagaatc ttgctccata aaaattccgc atgaggtcaa
    29521 gcgatgacat tttgtcgctg ccgattgtcc ggagccgtgg gatttgctat gtcagcggct
    29581 tcgccgcttg caatgacacg ctatccgttc acggtacaac aacctttctt catcaacccc
    29641 ttaatgcact cttctctgtt gatttaaaac gtttaatggt tattatatac ccgatgagca
    29701 aggaaaagat tccaacaaca ccggcggttt tagccctcaa agcacaacag gctgatttca
    29761 ctctgcatac gtacccctat gaggaaaggg gaggaacaaa agcatccgcc cggaaactgg
    29821 gcgttgacga acacatggtc attaaaacgc tggtaatgga ggatgaaacg ggtaaaccgc
    29881 tgatcattct gatgcatggc gatcgggaag tgtccaccaa gtccctggcc aggcagatcg
    29941 gagccaaatc cgtcacccca tgcaagccgg ccgtcgcgga aaaacatacc ggttataaag
    30001 tcggcgggac atcgccgttc gggaccagaa aagtgttgcc cgtgtacatg gaacagacca
    30061 tcgccgattt gccggatatt ttaatcaacg cgggcagccg cggtctgctg gcaaggatgc
    30121 cttcggccga actggcgcgt ctgcttaagc cggtcatggt cggcgttgcc atatgatttg
    30181 cctttttcgg gcaaaaaaga gataatgcct ttgaggataa atgctatgcc tgatgacgtg
    30241 attatccgct gtcttaactg cggaaccaaa aacagaatac cgaaacaaaa actgcaggga
    30301 cgatcgatct gcggcaagtg cggcgcttcg ctggatgaac tcatcatccg ctgcctgaaa
    30361 tgcagcacga agaacagaat acccgaggac cgattaaatc aacggccttt atgcggcaaa
    30421 tgccatgaac cgttaatcat tcctcagcaa aaagttttgc cgttcgatgt cacagaccag
    30481 acctttgccc gcgaagtgtt gacgtccgac tcttcggttc tggtcgattg ctgggcgccc
    30541 tggtgcggcc cttgccggac cctgtccccg attattgacg aactggcctc cgactatgcc
    30601 aacggcgtga aagtcgtcaa gctcaatgtt gacgaaaatc cgctgaccgc ctcgcaatac
    30661 ggcatccgca gcattccgac catgcttttt ttcaaggaag gcaaaatggt gcatcgtgtg
    30721 accggcgcac tgcccaaaga agaaatcgag cagcatctct taacgatcat caaaaccaac
    30781 tgaccctgtg gagtattcat ggccgacaac aaatcctcta tcgtctacca atgcaaaatt
    30841 tttgatgtcc gggaagagga ggtcaccttc ccaaacggca aaacggccag acaaagctgg
    30901 atcaaccatc accccacggt ggccattgtt gctgtcaatg aaaaaaatga gctgctgctc
    30961 atcaaacagt atcgcaacgc cgtgaaacag gaccttttgg aaataccggc cggatcgctc
    31021 gatgggatcg aagaatctcc tgcggtttgc gcgcaacggg agctggccga agaaaccggt
    31081 ttcaaagcca aatcgctgat caagctctac gaaggctatc tgctgccggg ttactgcaac
    31141 gaatacatgt atttttttct ggcccttgat cttttcagcg caccgcttaa tcccgacgac
    31201 gacgagttca ttgaaaccat acccgccacg ttttcccaag cacacaagct catcgacaga
    31261 ggcgtcatca tcgacgccaa aaccgttctg ggcattttgc tggctgaaaa acttttaggg
    31321 cggcaaaacg cctgatggat tcttgtttcc cgagaaagct tcttgccaga gacaaaaagt
    31381 tttgttaggc ttttacacga ttccgatatc cgttaatcca tgaggaggct gcgccatgat
    31441 ctacaacgaa gaatttgaaa ccatgccccg cgaagtcatc aaagccctgc aggttaaaag
    31501 actgcagcag gttctggagc gtgtctatca caccgtcggc ttttacaaaa aatcctttga
    31561 tgccgccaaa gtgaaacccg atgacattcg ctgcatggcc gacatgaaaa agcttccgtt
    31621 caccaccaga aaagatttgc gcgacaacta tcccttcggt ttgttcgccg tgcccatgag
    31681 cagcgtggtc cgtctgcacg catcgtccgg aaccagccgc cgctctgccg ttttcggcta
    31741 caccaaacgc gacatcgaca cctggtcgga tctgatcgcg cgctcgctgg tcgccgctgg
    31801 catcaccaaa aacgacatca tccataacgc tttcggctac ggcctcttca ctggcggact
    31861 gggactgcat tacggcgcgg aaaaaatcgg cgccagcgtc attcccatct ccggcggaga
    31921 cgtcaaacga cagttgatga ttctgcagga tttcggtccc accgtgctat gcgcgacgcc
    31981 ctcctatgcc ctgcatctgg ccgaagaagg caacgccatg ggaattgaca tcaaagcgct
    32041 gaaactgcgc gtgggcattt tcggagccga accctggacc gacgccatcc gcgatgacat
    32101 agaaaaagca ttcggcatta ccgcgctgga tttgttcgga ctgtcggaag tgatcggacc
    32161 gggcatggcg atggaatgcc tggaaggccg ccacggcatg cacattttcg aagaccactt
    32221 catcgttgaa accattgatc cgcaaacagg cgatgtgctt cccgaagggg ccgagggcga
    32281 actggttttc accacgctga ccaaagaagc caatccgctg gtccgttacc gcaccggcga
    32341 catatcgcgt ctgattaccg aaccgtgccg ctgcggccgg acccacatca aaatggaacg
    32401 agtcctgaag cgtagcgacg acatgctgat tatccgcggc gtcaatgttt tcccgtcgca
    32461 gatcgaagcc attctggtgg agatcgaagg tctcgagccc aattatcagg tcatcatcga
    32521 taaagcaggg gcgctggatg tcctggattt gcaggtcgaa gtcaacgaca aaatcttcag
    32581 cgactcgggc agcatcaaac aattgcagaa aatcgaacaa agaatcgtca aggacatgag
    32641 agattatctg ggtgtttccg cacgcgtcaa gcttgttcag cccaacacgt tgaagaaaac
    32701 cgacgccaaa attatcgaca aacgccgtat ttgattaacc acaaaagggg gttgccatga
    32761 aagtagaaca aatatccgta tttctcgaaa acaaacccgg cacgctggaa gacgcaacgc
    32821 gcgttctgaa agaagccaat atcaatatcc gcacattgtc cgtagcggaa accgtcgatt
    32881 tcggcattct gcgcctgatc gtcaatgacg tcgaaaaagc caacaaagtc ctcaaagaca
    32941 acggtttccg tgtaagcaag acccccgtcg tcgccgtgga agtccccgac acaccgggcg
    33001 gactgcacag catcatggaa gtggtgtcca aagaaggcat caatgtggaa tacctttacg
    33061 cctttgtgga aaaaagcggt cagaacgccg tcatcatctt ccgcttcgac aaccccgaaa
    33121 aagccatcga tgttctgacg aagaagaatt tcaccgtcat tccgggaagc aagctttacg
    33181 aattttagtg agactcgaat cttacaagcg cagcacagta agattcgtta gtcgaactac
    33241 cccaactgcg cagcagttgt gggtaatgac atcatctaga gatataatga actgaaactt
    33301 gatggttgaa ttatcctagg agcgtagcga cgcaggataa tgagtcccca aatttctgaa
    33361 acactgtgca cagtaagatt cgttagttga acgaccccaa ctgcgaagca gttgcggata
    33421 ttgacatcct ttaaaaaaac ggtggatcaa taattttatc caccgttttc actatccacc
    33481 agccaccatc aactattaac tattcacatt ttcttctgcc acggcttggt cttccaccgc
    33541 tggtggaacc agaaatattg atcggggtat tcgcggataa cctcttcaat gatctttgtg
    33601 aagcgttgcg tattcaccac caagtcgttt tcgtaattat ccgtacaaac cgcttcaatc
    33661 gtcggtttga tgataaattt atatttgcct gatttttgtc gggccataaa agcgggaaga
    33721 acgggagcgc cggaacgcat ggccatcacc gccaggccga ctcccgtaca ggcggtgcga
    33781 ccgaagaaat cgacaaacac gccttcataa tgggcgacgt tctgatcgct gagaatgccg
    33841 atcaggttat tttcgcgaag caactccata attctttttc ccgagccccc tttgggaatc
    33901 atatcgttgc cctgcatggt ccgcacatat tccacgatat tatcgatgac gtgattatcc
    33961 agcggccggt acacaatgta catcggtttg agatacatcg gaccggcaat cgtcatcaat
    34021 tcccagttgc cgaaatgcgc cacaatcgag agcattcctt ttcctttggc aatacccgcc
    34081 tgaaaatttt ccacaccttc aacatcaacc catttatgga tattgtcttt ggtcatgtaa
    34141 ggcaaatcga aaaactcagc gacaacgatg gcaaaatggc ggtaaacgcc tttggcgatt
    34201 ttgataattt ccttcgtgtc cttttcgggg aaagaacgca tcagattatg cagcgtaatt
    34261 aaacggcttt ttgtgccgat gtgataaaaa agaaaaaaaa tcgataaaaa cattcccttg
    34321 cgcacacatc ggggaatgcc gtgaaaaaca gaaagcaata tgtgggtggt tctggttttt
    34381 tcaactggca tagttattta tatttacagc ggcttgccgc attccttgca tttgccgtcc
    34441 ggaccgatca caccgatgca tgatccgtcg gagcacaata ctcttttatc ccaatcgtca
    34501 tccgagtgat catcggagga atctggcttt tccgaactta cttcatgaac gatttcattt
    34561 attttgcctt ccggcaattt tccttcgtaa ggtttgccgc attccttgca cttgccgtcc
    34621 ggaccgatag tgccgataca ggactcatcg ctgcacaata ctcgcttttc ccaatcttca
    34681 ttttcatcaa aaatgatttc cgtcacggcg ttctcccgga atttttttca ctgcgaaatc
    34741 tttgcaccat gcttaacttg tcatttcgaa cgtatgtgag aaatcttaaa gatccctcgc
    34801 tacgctcggg acatggattt ctcgtcgctt tgctcctcga aatgacacga gcgggtgtta
    34861 tgcaaaacgt ttaactcttc ttacatcagc tttgcaattt ttaccacaaa acagtgcatc
    34921 tttgataact tcgtaaaaag ttcagaggca aggcgtgcga agcccgatta aaataacacg
    34981 cattttcgtc aggcatcatt gccaagtcgc attcagaagg ataccgcggc ggcagtgaaa
    35041 ctcgaattgc cgttacgaag taaacggtaa ttccttagtt gaactgtcct gcctcagctg
    35101 ggcgaggagg aggctccgca ggcgtatatt ataatacgtt gaggaagccg acgacgctgc
    35161 caacaaaggt agccgcatga aggccgtctt taccttaagc cttgataact tcgtaaaatg
    35221 tccagaggca aggcgtgcga agcccgagga gtgaggcgta cttttggcgt acgccgcaac
    35281 aacgacggct gaagcacaac gcagtatctg ggcgttttac gaagttatca cccttttatg
    35341 cacgaaaggg attttaaacg tgccactttt ctggcaattc ccgcctcatg catcacctgc
    35401 acaacatcct gcacattttt atacgcttcg ggcatttctt cactgagcgt tcccttggaa
    35461 ccggccatca ccaaaacacc cttttccgaa agttcacggg caatggaacg cccgcggctg
    35521 tttttgatcg cctgcgaccg gctcatcacc cgtcccgctc cgtgacaggc gctgccgaag
    35581 gtttcggaca tcgctttttg ctgacccacc agtacataag accccgttcc catatcgccg
    35641 ggaatcagca ccggctggcc gacttttctg tagcgcacag gcaccgcctc atgccctgca
    35701 ggaaaagcgc gggtagcgcc tttgcggtga acgcaaagtt tttttatcac accaccgata
    35761 ttaaacgatt ctatcttggc aatgttgtga cagacgtcat aaaccaatcg catgttcacg
    35821 tcgcgcggcg acatccgcag cgttttctca aatacttccc tggttttgtg catcaggatt
    35881 tgccggttgg cccaggcgta attggcgccg caggccatgg ccgccagata gtttttgcct
    35941 cttcccgaag aaagataagc gcaggccaac tgcctgtccg gaagcgcaat ccccgtttcg
    36001 tttgcatgtt tcaccatcaa cgccaggtaa tcatcgcaaa tctgataccc caggccgcgc
    36061 gaaccggtat gaatcatgac ggcaatctgt cccttgtgca aaccgaaggc tttggccgct
    36121 tcttcatcga agatttcctg cacaacctca atttccagaa aatgattgcc ggaccctagc
    36181 gttcccaact gctgcttgcc gcgctccaga gcacgggctg aaaccagttc gggatcagct
    36241 ccagatatcg cgccgccgtc ttccgtagtt tccaaatctt cgtttaaacc gaagccggct
    36301 ttgatcgccc aggcggctcc ctcttccagc actttcttct gatccttgcc cgataacttc
    36361 acggaaccgg tcgagcccac acccgaagga atgcgctgat aaagggcggt tgttaaatca
    36421 ctgagccggt ttttgatatc cccaagcgtt aaatttgtgg tcatcagacg gcagccgcaa
    36481 ttgatatcgt agccgacacc gcccggagaa ataataccgc catccagatc gaaggccgcc
    36541 acgccgccga tgggaaaacc gtaaccccag tgaacatccg gcatcgccag agaaaaattc
    36601 acaatgcccg gcaggtgcgc gacgttggcc acctgcttcg cgctttcgtc gccctttaaa
    36661 agctgaaaga gtttttcatt ggcgtagatg atccccggca cgcgcatgcc gcccgtctgc
    36721 ggcaacagcc agcggttgtc atccaatttt ttcagtttaa tatcagacat aacaaattct
    36781 cttcaaatcg ccataccgtc gacaagattt atgtcacttc gaccgaaggg agaagtctta
    36841 atattatcgt ctttaagatt cctcaccccg acacgtcggg attcggaatg acattaagtt
    36901 acggattttg tcacacatca aaaattattc tcgccttcca gccagattct actcttttta
    36961 cagtcgcgcc ggaataggtt accgctttaa tctcggtttt aatcgaatgc tttttgttat
    37021 taaacgattc tccggttaac cgtgcctgta attttttatt ggaaaactct ataatttcgc
    37081 aactacccgt tatgaaatgt tcgccattga acagatgaag caactctcgt aaaaaattga
    37141 tcagcaaatc ggcaacatcc gttccttcga ctgttatttt tttctgccgc tttgctgttg
    37201 cccggcctgc tttgtgctca atcatcacat caaacaaagc cccggcggta ttcataaaca
    37261 gctcttttcg ggtccggcca taaacttcaa cgccgatatc ggctgtatgg tcaaagaatt
    37321 tgtagttcga catggagtga gtatagctga atcttgcgta ttgaacaagg agagctgact
    37381 gctcggcaac tttcatgaca agctgatctt gagttaaaca ataggtgtta gccgattgtt
    37441 taacagtcac aatgatttaa cattaaatag aaccgtcccc aatttctctg agggactcct
    37501 ttctgatgtg cgtgttggtc gtgcttcaca cactaccaga aaatcccttc atgtccgttc
    37561 tttatctatg agtggtgcac ttgcaagtag aatgcacctt agctggtcag ccttttctaa
    37621 tataatcatg tccccttttc gagtacaacc gtccctattg ttttctctcc ccagattcaa
    37681 tggattcttc ggctgcatta gagatgtccc agaatccttt gcttgccctc gacaactgtt
    37741 catcgagcat gtcccaagca aagccagcgt tccgcactag gagacccata agctctacca
    37801 gtctcttcat tcgatcgatg gtggagcgca gatcacccca tttgatacca agcgttccta
    37861 tatccacgaa ctgatacttg tcggcaacat acctgacttc tttgtgcgca gataccttat
    37921 ctcgaactat tcgaaaagct ttgatcttct tgctggtcga caactctgcc cagattgcca
    37981 ttgcctcaca gtagagtttt tcaaactgct ctcgccgttc tgcttcctca cgcagctcaa
    38041 ttcggtgaag tgcctcaatg atctcaggct ctgtctgctc ttcgatggat ggaatcttcc
    38101 atatagcgaa tcgattctga agctccttgc ggatcacatc gtctgtgatt gctcgtatta
    38161 ggttgtgtag gctgggagtt ttatcatcgt cgtcaagcgt caagtttgcg atatcctgcg
    38221 aacacgacag aaataacgag tgtttaagta tgcaaaatcc tctcgcttga ttgccggagc
    38281 atcgctcttt cggcattgat tcatcaaaga gcataggctc taaaagagcg taccgttcgc
    38341 gcagttgaat aaatgcatcg agtaagtggg aagcgtgggc ttcaagcctt tcaatatgag
    38401 ttggcattgg tctttatcct actaacatgg tattcaccga gcgtggctct gctcttttga
    38461 aataacactt taatttctat tactcataaa tgtcatcaac atagacatgt ggtccaacat
    38521 agtatgcaaa caacagcaat aaagatgcag caagaattgt taatgaaaca tgtattacag
    38581 tatcttcgat attatcaaga tgtggttctt tatcaataaa gagatatctc ccacaatttt
    38641 taactatggc caatacaatg accgctatca acaatattac aatgtaatga ctaaaccaag
    38701 aacctaggaa gcctagaaaa ccattttgcc aaggagggtt tttatattga ataatgtgtc
    38761 ttgcatagca aaagaaaaat gctgaacatg aaagcactat accttttata gagaatagtg
    38821 atctcaattt attcattttt ttatttccta aatttaacat tgtctagatg gctcggctat
    38881 gccacggggt tttaaccctc cgcttctccc acgtcgcgtt gctcctggga tagttcgctt
    38941 tacgcttcga gcgtcactac gctcgctctc agcttggctc actactattc ctcagcaccg
    39001 ttgtcttcgc cgttggtgtc agcttcatcg tcaccattgc cgtcgtcttt gccttcggct
    39061 tccgatgtgg tgcgggctac gccgaccagt ttttcttcgg gctccagatc aatcagcttc
    39121 acgccctggg taacacggtg attgacggga atttcctgga ctttcagacg aatgactttg
    39181 ccggcactgc tgatgagcat gacattatct tcaccgacaa cctgcaagac gccggaaacg
    39241 ttgccgactt tttcgacggt tttcagatta atcgtgcctt tgccgccgcg tgactggaca
    39301 cggtattcat ccaccggcgt gcacttgccg aaaccgtttt cggaaacggt gagcatgaac
    39361 gtgttctggg cggggatat
//
LOCUS       DAOSME010000017        38640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1415_length_38640_cov_19.017831, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000017 DAOSME010000000
VERSION     DAOSME010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 38640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38640
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1415_length_38640_cov_19.017831"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLG98_04070"
     CDS             complement(<1..272)
                     /locus_tag="PLG98_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOE32459.1"
                     /translation="MDEEQIKQEMIDYLMKKITGGLSDKVVDYGNNPRHCGTLTKPDG
                     YAKITGPCGDTMEMFLRIRNDKIEDIAYTTDGCMTSHAAGSAATVMA"
     gene            complement(338..736)
                     /locus_tag="PLG98_04075"
     CDS             complement(338..736)
                     /locus_tag="PLG98_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOE32460.1"
                     /translation="MVNVTEEIKESLKAAKIAYLATAAKDGTPNVVPIGAFKFMDGET
                     LLISDQYFSKTLNNLKSNPKIALSWWAEKGGFQIKADITIHTDDDIFRQNVEWVRGIK
                     DTLKPKSAIVGKITAVYAVRSGPDAGKQIL"
     gene            1060..1623
                     /locus_tag="PLG98_04080"
     CDS             1060..1623
                     /locus_tag="PLG98_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32461.1"
                     /translation="MSNVEKVKGTMLIDQVRMIRGNKDKDWRNYLTSEDWAIVKGMVM
                     PASWYPVETYKRCGRAVFQVLAGGNTDIVRLRGKMRGKELFETTYKHLLMERNPMNAL
                     SSFVNLYGQLFMPNPLGFKKMTDQHAHVSYEYEDKNDPGNLPFCYQLMGHFDSLIEMT
                     GGADVKIEFAKKMWEGASETVFDIQWQ"
     gene            1675..2235
                     /locus_tag="PLG98_04085"
     CDS             1675..2235
                     /locus_tag="PLG98_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32462.1"
                     /translation="MNVKGIIFNTGKSTIIKVFGQGPWDAFMTKLVAKDKFFGNVIMS
                     VTPIPLDKFIFFLDELVKEFFNNDMMQYVTFGKVAAQFALSPDGLYKSYLLTKDTKQF
                     VESVMPKFWSTYFDEGTVTTKYENNITHLKITGLNFRHNYFEYLIMGFFQKSLKMFGK
                     KNVPKRIRSRASGDDDVYFQFELKDS"
     gene            complement(2413..3258)
                     /gene="ccsB"
                     /locus_tag="PLG98_04090"
     CDS             complement(2413..3258)
                     /gene="ccsB"
                     /locus_tag="PLG98_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOE32463.1"
                     /translation="MNHTLILSYVTFIYFASFVFYLFRMILKKEFWGYAASLLAWLGL
                     FAHTLAMMLRWRASYDLGFGHVPITNLYESLVFFAWSIIMLYLAIEWRIKSRILGTFV
                     VPVAFFVMAYASIAPGVDHSIEPLIPALQSNWLTTHVLTCFMGYAAFTVAFGCGLLYL
                     WTEKKETRETNPASHPKENSSVLNTLDVLIYQSTSLGFIFLAMGIITGSVWAHYAWGS
                     YWSWDPKEIWSLITWLIYAIMLHARFVRGWRGRRMAMMTIVGFVSVLFTYLGVNFLTS
                     LHNYI"
     gene            complement(3255..4592)
                     /locus_tag="PLG98_04095"
     CDS             complement(3255..4592)
                     /locus_tag="PLG98_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOE32464.1"
                     /translation="MKKANFIWSFLASVKLAIVLLFFMVVIFIVATLVPPQRMDSEWL
                     WLNDLYHSKMFYVLMGLFSLNLMVCSIQRIPFAIKQYKTPSFPPPSGLYENLAPNRII
                     VTDKKMETVSRAVESLLKSKLFSVKETKLEKGKMLYRESGRLSLFGVYVVHLGVLMII
                     AGMVIGSVFGLEADLRLSEGEETNIIQLRNGEGEHPLDFSVRCDKFVVEFYDTGVPKT
                     YRSELSFIKNGQVVRQGSVLVNHPLSFDGLRFYQASYGVSEKSRARLTYSNAGAESPE
                     ISAGQGDTFDLPVQKAKATVLRVEENMMQFGPAVKLNIETDKRNIQFWMFQHIQDIAD
                     VNPGLFSAVPLFNPGLFKPLVFSLRGIEGKYYTGLQVVRDPGVPFVLAGGLILIAGMM
                     VIFFIAYRRIWFLLEQDPAGVRIRVAGRSHRYNEALQRRLDDLCAWMEKELAA"
     gene            4784..6049
                     /gene="nrfD"
                     /locus_tag="PLG98_04100"
     CDS             4784..6049
                     /gene="nrfD"
                     /locus_tag="PLG98_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOE32465.1"
                     /translation="MADSGKKRFLPHIKSAFERVLSGGWIYLLFVGLFGIFLLIGIGA
                     GIHALFIAGSRHAYATSREIPVAILIATYVFFVVASTGLCLVSSLGHVFGVENFLPLA
                     KRCVYLAIITILSGFLVIGLEIENPFRMAIYNVISPNFSSNIWWMGTLYGFAVVFLTI
                     EFILLFNEAHHHKAVIAGFLGVITEVAANSNLGGVFAMMHGRPYWYGPYFPVYFILSA
                     LITGCAFILFFFFLAHKAVNKKPFMESTQTALKAVSKLTLLLLAVMMIFTAWKVLTTS
                     VGSPAAAAALSVLTEGPYSFNFWTIEILVGMVIPFVLILASRGRNLTVMFIAAVMMIF
                     SLFFTRLDMVVVGQIVPQYFEVGVVEYSKLHTYSPSIHEILVVLGGISFCLLAFLLGE
                     KVFSGFADSPQTDIMKEKTTDVAGVEELT"
     gene            6046..6783
                     /locus_tag="PLG98_04105"
     CDS             6046..6783
                     /locus_tag="PLG98_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE32466.1"
                     /translation="MTDKASHKNIPESPFDAERRAFLKIGLKITGVLAGGSILSLVSN
                     INKAYSLDLFREKYPYKPHYAMVLRQDRCIDCEKCMKACIETNHVPDYGWRTKILKRD
                     LSSALDQKWEFIPILCNQCNNPPCVRTCPTTATYKDKTNGIVQMNNKKCIGCKSCIMA
                     CPYDARYYNEEKGAIDKCDFCFESRLSKGINTPACVEACPSDVRIFGDLSDPSSRVFQ
                     LVHQLQRPVWVLRQEIGAKPNIFYMKG"
     gene            6794..7546
                     /locus_tag="PLG98_04110"
     CDS             6794..7546
                     /locus_tag="PLG98_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOE32467.1"
                     /translation="MKRNLVLIILLALFCIANVGSTGKSSFSDGGGILYTQPVKSVIF
                     RHQHHVDVKKISCEKCHSGLFEMQALLAQEKKDFIMDSLYKGRYCGACHNGKDAFASD
                     KQCARCHVRISEKDVGHVKGKAKPYKAPVYSTVATLGKDQRKVPFNHEKHASLNCMDC
                     HSKLFQIRKGSTKIALETHQSQKYCFNCHNGKKEFSWNNCTKCHQNWKDIKPYGILTA
                     RADRGSCVACHTNPDEMKSLVKPPEIGGEAEG"
     gene            7637..8605
                     /locus_tag="PLG98_04115"
     CDS             7637..8605
                     /locus_tag="PLG98_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32468.1"
                     /translation="MDCIACHQGNKLAKTKEKAHQGRIARPSDNLKTCGICHITAGQN
                     YAKALHYTSLGQREGVKHRFSAAELKTFDQKVFEKSCRSCHASCGSCHVKSPPVGGIT
                     TGLLQGHKFVKKNEGKTCASCHGGRVYPEFTGEYGGTPDVHYQKGMMCNDCHNKQEMH
                     GDGKLHKSKNDVKDRPKCLSCHQDKGNQVAHTIHKDKVSCQGCHSSGQYRQCYSCHAV
                     HGSKAEPGFILGLNPRDRKTLTTLRVIPTIRSTFEPNGIKMENFDALPNYWNSPVHNI
                     KKRTNRTRSCDACHVNKEGFLKPEGLIKDGSKENEKLIYDIKPISK"
     gene            8622..9548
                     /locus_tag="PLG98_04120"
     CDS             8622..9548
                     /locus_tag="PLG98_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE32469.1"
                     /translation="MKRMRFLKVLLVSLLILLPAAVLAQGLSPIVSTDDLAKIVKDPN
                     VIVIDIRKVEDYKAGHIPGAIGVFYNNWAPGMGGLKNEMPADDDLMDLLTDNGIQPDS
                     TVVVYGTTGTPPDRVDVTRVAWALKYAGVQKVSVLSGGFEKWAVREKKEVSTEITKPK
                     TKDYKGKINKAILVNKDYVKNHVGKAMIVDAREPAFFKGEQKLPFVAKAGRVKNSVNL
                     PTIQVFDKYPPGEHMEPCCWTYKDVATLKNIASGVVGSDIDKEIIVYCDTGKVASAWW
                     FILSDVMGFKNVKIYDGSMEEWMNDASAPTEP"
     gene            complement(9627..10550)
                     /locus_tag="PLG98_04125"
     CDS             complement(9627..10550)
                     /locus_tag="PLG98_04125"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOE32470.1"
                     /translation="MKNHALSKLLTILLFILLLPIAGQTRDIEPIVSTDWLLANLKNP
                     KLIILDVRRVEDYREGHIPGALNAFYRSWAYQKDGLFTEIPETDDLDDTIGYFGIGFD
                     SRIVVTGCMDTPRLSYQSARVACTLQYAGIKNVAVLNGGMNKWITEKKPLSQEIMHAK
                     AKPFKGLYSRARFADKDYVKERLGKAIMLDVREREFFTGEKKMDCIPMMGRIPGAFNM
                     PTSCAFNPDKTFKTTEELAAISEAAIGRDKEKEIITYCDIGQCCPIWSYILVEVLGYK
                     NVRLYDGAMQEWMQDPQAPITKNADANVTPK"
     gene            complement(10684..11229)
                     /locus_tag="PLG98_04130"
     CDS             complement(10684..11229)
                     /locus_tag="PLG98_04130"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(11011..11013),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOE32471.1"
                     /translation="MRITKFVFFVLVFFILSACASTGAKNASPVSGQVAPDFTLSDQK
                     GNIWKLSNAVKNHRAVVLAFYPKDDTKLUIRQFVEFQQNIKKYKAKKIKILGLSRDSA
                     ESHRKFIAKHSLKNMTLLVDEKGKIAKLYDADHWLLPLSKRVYVIVDKKMNIIYRNDM
                     GFALLPDQTQTLMGEIDRHIR"
     gene            complement(11298..12023)
                     /gene="prxU"
                     /locus_tag="PLG98_04135"
     CDS             complement(11298..12023)
                     /gene="prxU"
                     /locus_tag="PLG98_04135"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083760882.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(11769..11771),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOE32472.1"
                     /translation="MAEEIKAGCARPTGGPVGEKPDAKKETSQPTPVKEVKSMVTVGK
                     KAPDFTAPAYHKGKFINVKLSDYLGKWVVLCFYPGDFTFVUATEISSVGEKYPEFKKL
                     GVEVLSMSVDSVFVHKMWDDHELSKMVKGGIPFPMLSDGGGKVGDVFGVYLQDAGVEA
                     RGRFIIDPDGIIQGFEVLTPPVGRNVMESIRQIQAFQHVRKTKGAEATPSGWKPGKPT
                     LKPGPDLVGKVWEVWKTKMAFDD"
     gene            complement(12061..12357)
                     /locus_tag="PLG98_04140"
     CDS             complement(12061..12357)
                     /locus_tag="PLG98_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOE32473.1"
                     /translation="MPEFGTPFSGLKKDRKLTKEELIRAIRFMVAAEYEAIQLYMQLA
                     ESTDNKLAIEVLGDIADEERVHAGEFLRLLHELAPMKENSMRKALRKWRKKSRK"
     gene            complement(12467..14167)
                     /locus_tag="PLG98_04145"
     CDS             complement(12467..14167)
                     /locus_tag="PLG98_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE32474.1"
                     /translation="MAQRKRIIVIGGSAAGPKAAAKARRIDNDAEVIIIQKEADLSMA
                     SCGYPYYVGGFFNDRNALLCTPAGVVRNPMFYLNAKDIEAKVNTEVTNIDTKNRSVSF
                     KNTVSGETGTLTYDKLVIATGAVPRMPPVPGTDLQGITTLQSMKDADFLRNVRDEGKI
                     KKAVVIGGGLIGIETCEALQLAGIEITVIELLPQLLTFLDWELAKLVENHVRSKGANI
                     ITENGIAAFLGENGKLTAVKLQNGTELPCELAVIAIGVRPNVTLAKEAGLKIGELGGV
                     EVNEYMQTSDPDIYAVGDCVETLNRITGKKVHAPYGDLANLQGRIAGENAASGNSVTF
                     PGTIQTGICKVFDYGAGSTGLSETMAKRLGYSDIITVINASPDKPGFMEGKLLVTKLV
                     AEQKTGRILGAQCIGPGNVAKQIAQWAMAIQGKMTVEDLINADLPYAPPFSLAIDHFI
                     ATAHIMQNKMKGRMKGISCKEVYDKIRAGEKPFLIDTRGPEEFEEMRLGIGETLIPLG
                     AIRKRLKELPEDKNREIILYCKISLRGYEAALALEGNGWKNVKVMEGGIMAWPFPREK
                     "
     gene            complement(14417..15610)
                     /locus_tag="PLG98_04150"
     CDS             complement(14417..15610)
                     /locus_tag="PLG98_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOE32475.1"
                     /translation="MSYAKLSDWNKCTTCGECLVQCPVMHMDKPEAVAEMTGLLKTGY
                     SEKIFRECTFCFSCNNYCPEGLRLHEFILEKFMENRKKKGKISAVYPYLFNGMKMSMW
                     KDIYAMLSREEQSILKKWSEIPPPSKEILWVGCIGRISCFDIENSAVLQELPKFGPPD
                     LCCGELAYRLGSWKAYTETIEKTLKAFEKLNIERMVCYCGSCYNYLSNILKNVYGREL
                     PFKLISLYEWLNEKRESGKITVKQPLKLKTTLHESCYVSELGDGFARDLRAVYKSAGA
                     DIVELPHHGECNLSCGAVSVLRTLNLPKSLFKEQWKKYKEVKETGMKDMAVNCPGCFI
                     TLSFTNRIAGIKLHYMPDELLKAYGDTITKPLSRRIPLFIRAFIKHPTLPLRHVDAEI
                     SPASS"
     gene            complement(15629..16363)
                     /locus_tag="PLG98_04155"
     CDS             complement(15629..16363)
                     /locus_tag="PLG98_04155"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOE32476.1"
                     /translation="MNKTIWKPEPEINLILKDYKTFGILSCGTCANLSYTGGKTGVEV
                     LKAHLLKNHKQIKLDKVILAACPEEIMRQALRANKKALEKCDALIVLSCAAGIKSANS
                     CRPDLPVINVLDSVGSAAVSSSDPVIAHSLCRSCGHCVLTCTAGICPLSECPAKSKYG
                     PCKSYSESNLKCALDPQINCVWHIIHERSNNLHLLPLLKELHSHKEDRNTHSTHEIKS
                     AGLWQKFFAWHAARIQKLEWVARQFR"
     gene            complement(16419..16901)
                     /locus_tag="PLG98_04160"
     CDS             complement(16419..16901)
                     /locus_tag="PLG98_04160"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOE32477.1"
                     /translation="MKQKAFQDYYPDDLSHCYGCGRLNEHGLQIKSYWDGEESVCVYQ
                     PDPRYMAIPGMAYGGLIASLIDCHSTGTAAAAKYCSENREMDTLPPIRFLTASLHVNY
                     LLPTPLGGPIEIRGKVKEIKRRKVVIESRLIAGGQICATGEVVAIQVPEALMPDHLKE
                     "
     gene            complement(16935..17111)
                     /locus_tag="PLG98_04165"
     CDS             complement(16935..17111)
                     /locus_tag="PLG98_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32478.1"
                     /translation="MKTKFETILDVKIYTIDAVEALPYNFRSSTNVIFDNEHVHVDIA
                     TDAQKMHAFLSSRL"
     gene            complement(17206..18156)
                     /locus_tag="PLG98_04170"
     CDS             complement(17206..18156)
                     /locus_tag="PLG98_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE32479.1"
                     /translation="MTTALKAVDRVEILTLQDNYIEITAMDSNAVVTRANPLKEGEIR
                     ASVLAEHGFSALVQTTLGNKTHTLLLDFGFSENGAAQNAETLNVDMTQVEAAALSHGH
                     SDHTGGMAKLVTMTGKKNIPFAVHPSAFKSPRYLKFGEELKINFPELTREMVKAAGLT
                     VVETEKPYLLLEDTILFLGEIPRSTDFETGWPLPHCQQDGKEVWDAIEDDTSIVMNLK
                     NKGLIILSGCAHSGIINTVHHAMKVTGINKIHAVMGGFHLSGPFFEPIIDRTTQEFKK
                     INPAYIIPTHCTGRKAIMEMEKQMPEQFILNMAGTKLTFN"
     gene            complement(18177..18515)
                     /locus_tag="PLG98_04175"
     CDS             complement(18177..18515)
                     /locus_tag="PLG98_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE32480.1"
                     /translation="MKIAIPLAEGKLTAHFGHCASFAIIEIEDQTKKIIKREDIAAPP
                     HEPGLLPPWLAKQGANMIIAGGMGQRAQQLFAEQGIQVVVGAPVETPEKLITDFFAGS
                     LQVGDNLCDH"
     gene            complement(18528..19424)
                     /locus_tag="PLG98_04180"
     CDS             complement(18528..19424)
                     /locus_tag="PLG98_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOE32481.1"
                     /translation="MEEKKTCDTCGDSDCAAKKQKKDESPEEYADRRKLQATLCRIQK
                     KIVVLSGKGGVGKSTVAVNLATALALNGLRVGLLDVDIHGPSVPTMLGLENETLKGGP
                     DGLFPVEFGGLKIMSLGFLLQNPDDAVIWRGPMKMGVIKQFLTDVHWGDLDYLIVDAP
                     PGTGDEPLSLCQLIQPLDGAIIVTTPQKVAAVDVRKSISFCKRLDVPVVGVIENMSGF
                     ACPKCGEITQILPTGGGKKISTDMNVPFLGAIPMDPAIAQSGDSGKVFIYHSSKTPTG
                     EIMQKIIDEITKDLDKTKILNS"
     gene            complement(19445..19828)
                     /locus_tag="PLG98_04185"
     CDS             complement(19445..19828)
                     /locus_tag="PLG98_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOE32482.1"
                     /translation="MSELVNLDETYATAKKHADSPHNMGPMDSFSGHARITGPCGDTM
                     EFWILVEEGKIRKVSFITDGCDPSRACGSMTTCMAEGKSIEEAQAISQKDVLNVLDGL
                     PEDHEHCALLATNTLKAACEDYLNQ"
     gene            complement(19840..20721)
                     /locus_tag="PLG98_04190"
     CDS             complement(19840..20721)
                     /locus_tag="PLG98_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32483.1"
                     /translation="MKELVIISGKGGTGKTSVTASLVALAKRSVICDCDVDAADLHLV
                     LTPQIKEEHEFISGHEAVIRQTDCISCGICKDVCRFNAIHSKQNFQGELLFAVDPVAC
                     EGCKVCVEMCPEKAIDFPDCNCGKWFVSDTRLGPMVHARLGVAAENSGKLVSTVRREA
                     RAIAEKENCELIIVDGPPGIGCPVIASVTGASQVLVVTEPTVSGEHDLERVLGLTRHF
                     QIPAAVCVNKWDINPDMTERIEENAAGLGARIAGRIRYDNAVTQAQIQKKSVVEIDAP
                     SGDDIQQLWKNLNLREV"
     gene            complement(20727..21356)
                     /locus_tag="PLG98_04195"
     CDS             complement(20727..21356)
                     /locus_tag="PLG98_04195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE32484.1"
                     /translation="MQKDIIENHIKYQKRVALYRTFGYNIDLERSFIIEKSLPVAGNI
                     LEAGTGKGYFTLALAQAGYKFFSLDISVAEQRFALLNLMYYGFQSQVTFLIADVESIP
                     CNDGFFDVIFAVNMIHHLSSIRKVCDEFTRLLSPDGKIVLSDFNEKGLAILDRIHERE
                     GRYHDVSGGTLAEMKTILVERGFTVREHQSDLQDMLVAGRTNLSPDIEE"
     gene            complement(21372..22322)
                     /locus_tag="PLG98_04200"
     CDS             complement(21372..22322)
                     /locus_tag="PLG98_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32485.1"
                     /translation="MSERVYQYVYGPVPSRRLGKSLGVDIVPYKTCTYDCIYCQLGKT
                     TNKTTVRLDYVPLADVLSEIEEKLLTKFECNYITVAGSGEPTLHASIGEIIGKIKERT
                     NIPVAVLTNGSLLYLPEVRQSLMKADIVIPSLDAGNEQLFQYVNRPHKDISFERVIQG
                     LIDFSKQFTGHIWLEILLLSGINGMAEEVKEMAAWVNKIHIEKVQINTVSRPAYEDFA
                     HPVDVRQLQKLARLFLCPVDILQNATSSDLSGINGVESSDKDILDLLARRPCTLEGIS
                     TGMGLHPHDVAKKLKSLLDKRLIIAMRAEQGLFYKIAERK"
     gene            complement(22324..22581)
                     /locus_tag="PLG98_04205"
     CDS             complement(22324..22581)
                     /locus_tag="PLG98_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE32486.1"
                     /translation="MPTYEYECSKCGLRFERQQSMKDAPIRICPECRADVQRIVSGGS
                     GFIMKSAGVSMRDTAKSCSFEETGTTCCGAKQRCGQAPCGD"
     gene            complement(22588..23430)
                     /locus_tag="PLG98_04210"
     CDS             complement(22588..23430)
                     /locus_tag="PLG98_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32487.1"
                     /translation="MKLAVASGKGGTGKTTVSVNLARVFKRDVCLADCDVEEPNAHLF
                     LKTSGHSEKTIGLPVPDVNEELCNGCGECAKICQFSAIVTFGTTPLVFPEMCHGCGGC
                     TLVCPTKAISEKNRRIGVVETSRTENITLVTGKLNVGVALAPPLIRAVKNTLPVDAPA
                     ILDAPPGTSCPVIAAIRDSDFVLLVTEPTPFGLHDLSLAVDMVRELNLPFGVLVNRMG
                     SGDNRVHEFCRKENVPILMEIPDDRQIAENYSRGILMVDALPQYRERFENLLEKIANS
                     KKKG"
     gene            complement(23427..24041)
                     /locus_tag="PLG98_04215"
     CDS             complement(23427..24041)
                     /locus_tag="PLG98_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32488.1"
                     /translation="MKAGMIAQELKLHAPFTMFGTVTGIVIMAAFIQFQVPREFSSGL
                     FWTMHPLHVLLSALVTTAMYRMHSRGNILQIIIIGYIGSIGIATFSDSFIPYIGEWLL
                     ELPHRGVHLGFIEKWWLVNPLALAGIALGFVISHTRIPHAGHVLLSTWASLFHVLMAL
                     GTSVDVVTTILIAFFLFLAVWVPCCTSDIVFPLLWINPKESKIL"
     gene            complement(24181..24672)
                     /gene="tsaA"
                     /locus_tag="PLG98_04220"
     CDS             complement(24181..24672)
                     /gene="tsaA"
                     /locus_tag="PLG98_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOE32489.1"
                     /translation="MINRQIIYHPIGIIHSEHTVAEKTPIQPVYACGCKGRIEVFEKY
                     ISGLSDLDGFSHVYLIYHFHQAPLVQLKVKPFLQDVERGIFSTRAPGRPNPLGLSVVE
                     LIRIEDNILYVDGIDILDGTPLLDIKPYTAKFDLHDIRKNGWQDEVDESTAAKRGKRG
                     YRS"
     gene            complement(24669..25031)
                     /locus_tag="PLG98_04225"
     CDS             complement(24669..25031)
                     /locus_tag="PLG98_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE32490.1"
                     /translation="MKIALTTSGVDLNAPLDSRFGRAPKFLIYDQAANTFEIVDNEQN
                     LNAAQGAGIQSAQNIARLGVKALITGHCGPKAFRTLQAAGIRIYNTSAATVSEALEQY
                     REGTLTEAKSADVDGHWV"
     gene            complement(25148..25522)
                     /locus_tag="PLG98_04230"
     CDS             complement(25148..25522)
                     /locus_tag="PLG98_04230"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOE32491.1"
                     /translation="MPRGDATGPIGMGPMTGRGAGYCAGFGAPGYMNHIPARRFGAGF
                     GRGAGFGGRGGRGGGFGFGNRFYAAAIPGWRRTDEYSAAVVPPDPHEEQQALRTQAEF
                     LQRELDAIKKRLDELKTDTQNN"
     gene            complement(25545..25784)
                     /locus_tag="PLG98_04235"
     CDS             complement(25545..25784)
                     /locus_tag="PLG98_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32492.1"
                     /translation="MNTNNRNPRGTGGGMSQGSGSGMGRGRGGGGRGRMGGMKAGGAL
                     GNCLCPKCGNRQPHDRGIPCTQVKCPQCGSTMIRE"
     gene            complement(25814..26362)
                     /locus_tag="PLG98_04240"
     CDS             complement(25814..26362)
                     /locus_tag="PLG98_04240"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOE32493.1"
                     /translation="MNQYIPKEIFFTKGVGTHREQLHSFELALRDAGIEKCNLVQVSS
                     ILPPGCRIISRTEGLKKLRPGAITFCVMSRCCSNEPRRLISASVGCAIPMDKKTYGYI
                     SEHHSFGYNEKQTGDYAEDLAAAMLASTLGIDFNIDESWDEKKELFKISGKIVGTRNT
                     TQSTVLKTKDYTTVLAAAVFVF"
     gene            complement(26402..26794)
                     /locus_tag="PLG98_04245"
     CDS             complement(26402..26794)
                     /locus_tag="PLG98_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE32494.1"
                     /translation="MKLALSVFKDCISTVFDSAEQLLIVEADTANPDKRTLQKFVATD
                     PAGRAAELKNRGVDVLICGAISRPMQALIMAQGIIVHPFVRGEVHAIITAYRNNTLGL
                     PVFSLPGCRRRAMGAGFCKQRGMRYRSR"
     gene            complement(26968..28338)
                     /locus_tag="PLG98_04250"
     CDS             complement(26968..28338)
                     /locus_tag="PLG98_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE32495.1"
                     /translation="MKKKMPLKQGSPDIGTKQDIILDSIADGVFTVDQDWLITSFNRA
                     AEKITGVPREEAIGQRCKDVLKADICEKDCCLRKTMKTGEQIINKKVAIINAEGHKLP
                     VSISTALLRDQKGKLLGAVETFRDISVEEDLRKAVLQKYNFADILSKNHKMLQLFDIL
                     PDIAAGGSTVLIEGESGTGKELIARAIHNLSPRRKTSFVAVNCSALPDTLLESELFGY
                     KAGAFTDAKKDKPGRFKLAENGTLLLDEIGEITPAMQVKLLRVLQEKTYEPLGATQSV
                     EHNVRVIAATNKNLEDLVRQGRFREDLFYRINVFKITLPPLRERMEDIPLLIDHFISR
                     FDLLQKKEIEGVSQEALSVLMAYTYPGNIRELANIIERAFIFCKEGRIEKKHLPESLF
                     ASPPQKIRTDASSLRDVEAAWLINALQKNNWNCRETARQLGIHKSTLYRKIKALGITL
                     PGQFPL"
     gene            complement(28367..28723)
                     /locus_tag="PLG98_04255"
     CDS             complement(28367..28723)
                     /locus_tag="PLG98_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE32496.1"
                     /translation="MKIALPSYQNQIDEHFGHCEYFTVFTIDQEKKIVEEEKVSSPAG
                     CGCKSNIAEVLAAKGVKLMLAGNMGQGAVNVLNSHGIDVLRGCSGNVKTVTESWLAGT
                     LKDSGISCTQHEHGCH"
     gene            complement(28784..29077)
                     /locus_tag="PLG98_04260"
     CDS             complement(28784..29077)
                     /locus_tag="PLG98_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOE32497.1"
                     /translation="MPRPKCHRHIMGAPDKKYFKPRGIPSMELEEIVLNMDEFEAVRL
                     ADYEHLYQKQAAEKMNISRQTFGRIIDVAHKKIADVLINGKALRIGGDFDFDE"
     gene            29334..30122
                     /locus_tag="PLG98_04265"
     CDS             29334..30122
                     /locus_tag="PLG98_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOE32498.1"
                     /translation="MQKHISNVHVHMPYSLLPKYLKIILQNKLNLEIYFHHQILNRLD
                     KSRCMETAKILESEGLKITFHAPFVDLRPGALDETIRRASIDRIRQVFDLAPYFHPLK
                     IVCHASFDDRYYVYGDDLWLESSLKTWKEFIGLADDHRTIIALENVYEKNPVILGKLF
                     RSLASDRICFCFDTGHFNVFSCEPLNVWFDELGRYTGHLHLHDNFGWQDEHLSVGHGT
                     FPFAELMQYIRNMKKKPTVTLEAHNQKNLWASLDHVKRMDFPGV"
     gene            30153..31127
                     /locus_tag="PLG98_04270"
     CDS             30153..31127
                     /locus_tag="PLG98_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE32499.1"
                     /translation="MLRYVLKRTLFMIPLLIGITLICFFVMRLAPGSPTDLQTEMNPK
                     ASQESKERLNALYELDKPIYVQYGSWLKKISRGDLGVSFSSDHRPVADKIMERLPITI
                     LINFLSLVIILAVAVPIGVLSAVHQDSLFDKVTTVLVFIGFAVPTFWLALLLMIFFGI
                     HLGWLPISGLRSLNYEYLNVWNQLIDLAKHLVLPVFVSAFGGLAGLSRYMRANMLEVI
                     RQDYIMTARAKGLSERDVIYRHALHNALLPAITIMGLSIPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPTIMGILLIGAILTLVGNLIADVSYAVADPRIRIS"
     gene            31141..31968
                     /locus_tag="PLG98_04275"
     CDS             31141..31968
                     /locus_tag="PLG98_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE32500.1"
                     /translation="MTAWTAIYKNKLMLTGGGLVLTLLIVSILAPWLSPYDPGQIDLN
                     NVLAAPSMEHLFGTDQLGRDVLSRMIWGARISLKVGFVATGLAILVGTFLGALSGYYG
                     GWIDSVIMRFVDIMLCFPTFFLILAVIALLEPSIWNIMIIIGLTGWMGVTRLVRADFI
                     SLRERDFVQAARAIGAGDARIIFLHILPNAMASILVAATLGIAGAILTESALSFLGIG
                     VQPPTPSWGNILTAGKDNIDIAWWLSLYPGLAILTTVVGYNLLGEGIRDLLDPRLKK"
     gene            32062..32229
                     /locus_tag="PLG98_04280"
     CDS             32062..32229
                     /locus_tag="PLG98_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32501.1"
                     /translation="MPNRVNAESISDDKRILKRKLMQGEISEKDIQALIKKLPDVSEN
                     AEEVRLDDDKK"
     gene            32236..33015
                     /locus_tag="PLG98_04285"
     CDS             32236..33015
                     /locus_tag="PLG98_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE32502.1"
                     /translation="MLIDSHAHLEMKEFDADRDDVISRARSEGVDFIVTVGTNLRLSS
                     RAVALAEQYENIFATVGIHPHDVAKAGEDTLDALKQLVLTHKKIVAYGEIGLDYFRNI
                     SRQEIQVEMFGQQLDLVKELNLPVVIHDREAHRQSLDMIQASGVRRGVFHCFSGDYDM
                     ARKCIDLGFYISIPGVVTFDKSGKIRDVVERVPLSSLLLETDAPYLTPVPNRGKRNEP
                     AFIVHTAKAVAAIKKISWEDVAAQTSVNTINLFGLKMIRNV"
     gene            33036..33731
                     /gene="queC"
                     /locus_tag="PLG98_04290"
     CDS             33036..33731
                     /gene="queC"
                     /locus_tag="PLG98_04290"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOE32503.1"
                     /translation="MKKQFKAVVLLSGGLDSATTLAIARDRGFEIYALSFRYGQRHAV
                     EMKAARRIARKNKVAGHLILNMDLRKIGGSALTADIHVPKNQTGSVLKRKIPVTYVPA
                     RNTIFLAYALAWAEVIGSHDIFIGVNALDYSGYPDCRPRYIAAFRKLANLATKAGVEG
                     KQKLAIHAPLIKMSKAEIIKKGMDLGVDYSLTHSCYDPSPSGEPCGECDSCLLRLKGF
                     REAGLKDPLKYCR"
     gene            33742..34383
                     /gene="queE"
                     /locus_tag="PLG98_04295"
     CDS             33742..34383
                     /gene="queE"
                     /locus_tag="PLG98_04295"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HOE32504.1"
                     /translation="MGYRIKEIFYSIQGEGFYTGRPAVLCRFSGCNLWSGKASDREKA
                     VCRFCDTDFVGCDGPEGGIFQTPEALTEKIITLWPEKPSDTLRPFVVLTGGEPLLQTD
                     RALLDALHHQHVEVAVETNGTIMPPEGIDWICVSPKAGTTMVLRSGQELKLVYPQQGA
                     EPEKYALLDFQHYFLQPLDSPNRQTNTLRALEYVLAHPQWRLSLQIQKILNVK"
     gene            34395..34754
                     /gene="queD"
                     /locus_tag="PLG98_04300"
     CDS             34395..34754
                     /gene="queD"
                     /locus_tag="PLG98_04300"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOE32505.1"
                     /translation="MEIFKVYKFDAAHRLPKVGAEHKCFELHGHSFRVEIHVQGPVDP
                     QLDWVMDFADIDRAFRPLLDQLDHQNLNDIPGLNNPISENIARWIWENLQDALPQLNK
                     IVVQESPESGCIYCGEK"
     gene            34913..35314
                     /locus_tag="PLG98_04305"
     CDS             34913..35314
                     /locus_tag="PLG98_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32506.1"
                     /translation="MDQKTIQKIKGTQQDGLLEELLREKAAVLTRASWAVEDVLEKMK
                     RIEGEIEIKTSLIQSMTHEADRAVLSRQKKLFCEEINCKIDEFNAIRRKAQLQYYYLI
                     VTREALGLRRHEMVQKIYRIPDKKKKIQVFF"
     gene            35314..35967
                     /locus_tag="PLG98_04310"
     CDS             35314..35967
                     /locus_tag="PLG98_04310"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOE32507.1"
                     /translation="MDRFRKPRMRMVETQIKARGISDVRVLKAMQTIPRHLFVDEGLI
                     EQAYTDSPLPIGEHQTISQPYIVALMTQALDLKGRERVLEIGTGSGYQTAVLACLADR
                     VFSVERIALLATQARRTLDALNIYNVAIRVGDGSYGWKEEAPFDAVITTAAVPAIPRY
                     LLDQLVPGGRMIVPSGGRDVQTLYKLTRSKENPEEIIQEDLGGCRFVSLIGESGWRK"
     gene            35955..36842
                     /locus_tag="PLG98_04315"
     CDS             35955..36842
                     /locus_tag="PLG98_04315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOE32508.1"
                     /translation="MEKIATRAKPVFFLLLVCLIFSCWHPRLQKAQAAGVYHLVKKQE
                     TVWSIARTYGVSVKDMVTINHIDDVNQIEEGSVLFIPSAERVIDDTTQIKKEKVDEAP
                     KTDIRDSGRTGDGKNSKKTGGKKIAAEAAPVKTGDLQDSATAKIRDDKPTDPKNQTVE
                     EMVVPDRKIKATRNKFIWPVQGEIKAHFGIQPNKTLHNWVKIVSASGKKIKAAESGTV
                     IFSSHLKNYGETIIIRHKDHFATVYTHLKKRLVKMDTPVKKGEAIAVLGEKDDDGAVY
                     MNFEVRLKGKAVNPLSYLP"
     gene            36907..38052
                     /locus_tag="PLG98_04320"
     CDS             36907..38052
                     /locus_tag="PLG98_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32509.1"
                     /translation="MKCYFEQGPIRPPSEAASLLIRVSRNCPWNKCAFCHTYRGTKFE
                     LRSVEDVKKDIQNMKEIADEIVRFSWKLGEGGRVNRNVLRAIYNSEDFYHDNIYSVAA
                     WLYFGGENVFLQDANSLILKTPDLVSILDFLKEKFPDVQRITSYCRSHTAARKSIAEF
                     EQLKKAGLSRIHIGMESGSDEVLSFIRKGVTSADHIRAGLNIRGAGIELSEYVIPGLG
                     GERFTRQHALETARVINAINPDFIRLRTLHVVPGTDLDELMNKGEFKPLADDDILREI
                     KLFIENLDVRGTYLASDHILNLLEELEGNLPDDRQNMLAAIEKYFKLSAEDRLVFRLG
                     RRSGSLRKLDDLADQATYGRLRSVVDHYAARGENLDEDIIKMMNNFI"
     gene            38429..>38640
                     /locus_tag="PLG98_04325"
     CDS             38429..>38640
                     /locus_tag="PLG98_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32510.1"
                     /translation="MRECVIIDGVRSPNSRAHNEKGWFRALRPDEVLTKVYDGLFARN
                     PKVKPEDIESVFVGCANITGMQNDIGR"
BASE COUNT         9608 a         9323 c         9384 g        10325 t
ORIGIN      
        1 gccatgacgg tcgctgccga tccggctgca tgggacgtca tacatccgtc ggtggtatag
       61 gcaatatcct cgattttgtc atttcttatc ctgagaaaca tttccatcgt atccccgcac
      121 ggtcccgtta ttttggcgta gccgtcaggt tttgtcagcg ttccgcagtg tctcggattg
      181 tttccgtaat cgactacttt atcggaaaga ccgcctgtta ttttcttcat aagataatca
      241 atcatttcct gtttaatttg ctcttcgtcc accgtgttgc tccttgttgc tcattcagac
      301 agaataaata aaaaactcgc ggcttatatc tcgcgatcta aagaatttgt ttgccggcat
      361 cggggccgct cctgaccgca tacacggcag taattttgcc cacaatggcc gattttggct
      421 tcagtgtgtc cttgatgccg cgaacccatt cgacgttttg tctgaagatg tcatcgtcgg
      481 tgtggatggt aatatcggcc ttaatttgaa aaccgccttt ttccgcccac cacgacagtg
      541 caattttagg attgctcttc agattattca gtgttttgct aaaatattga tcggaaatca
      601 gcagtgtttc gccatccata aatttgaatg cgccaatcgg aacgacgttg ggtgtgccgt
      661 cctttgccgc cgttgccaga taagcaattt ttgcagcttt cagactttcc ttaatctctt
      721 ccgtaacgtt taccatcaat tttacctcca aatgaagtga agtgggtgac gaaatcaaac
      781 tgttgtgctg tcttgttctc gcgtgtaaaa aaacccttcc atggtcgttg attatcataa
      841 aataacccgg cagagaaaca aaacttcatc gggtgtccgc cggtgccaaa gaggactttg
      901 tttttgcccg tctggcttga cggagagttg attccatcaa taaacgattt gccatgaaaa
      961 ggaaattgtg taataatatt aaattatgat gcagttcatt catcacgatg gttccgcaat
     1021 aaaaatgcag ttacaattat gactgtcggg aggttatgca tgtccaatgt agaaaaagtc
     1081 aaagggacaa tgctcataga tcaggttcgc atgatcaggg ggaataagga taaagattgg
     1141 cgcaattatc tgacgtctga agactgggca atcgtcaagg ggatggttat gccggcctca
     1201 tggtatcctg tcgaaacata taagcgatgc ggacgggctg tttttcaggt tcttgcgggc
     1261 ggcaatacgg atattgtgcg tttgaggggc aaaatgcgcg ggaaagagtt gtttgagaca
     1321 acctacaagc acctcctgat ggaacgcaat ccgatgaacg ctcttagttc ctttgtgaac
     1381 ctttacggac agctttttat gcccaatccg ctgggcttca aaaaaatgac ggatcagcat
     1441 gcgcacgtat catacgaata cgaagacaaa aatgacccgg gtaatttacc gttttgttat
     1501 caattaatgg gacattttga ttcactgatt gaaatgacgg gaggcgctga cgttaaaata
     1561 gagtttgcaa aaaaaatgtg ggaaggcgcc agcgaaacgg tttttgatat tcaatggcag
     1621 tagcgttttt tagaagactt taaatcagaa aacatatctt ccggaggttt tgaaatgaat
     1681 gtaaaaggca tcatttttaa tacaggaaaa tcaacaatca ttaaagtatt cggtcagggg
     1741 ccctgggatg cgtttatgac caaactggtg gctaaagaca aatttttcgg gaatgtaatc
     1801 atgtccgtca cgcccattcc gctggataaa ttcatttttt tcctggatga actggtcaag
     1861 gaatttttca acaacgatat gatgcagtac gtgaccttcg gcaaagtggc cgcccagttc
     1921 gcgctgtcgc cggacggtct gtacaaatca tatctgctga ccaaagatac caaacaattc
     1981 gtcgaatcgg ttatgcccaa gttctggtcg acctattttg acgaaggcac ggtgaccacg
     2041 aaatatgaga acaatatcac ccacctcaaa atcacaggtc tcaatttcag acataattat
     2101 ttcgaatacc tgatcatggg attttttcag aaatcgctca aaatgttcgg gaagaaaaac
     2161 gttcccaaac ggatcagaag ccgcgcatcg ggcgatgacg atgtttattt ccagtttgaa
     2221 ttaaaagatt cttaacgggt tgttttgaaa cttcgatcaa cgcaaaaagc ggtgcatccc
     2281 gcattgtaga gtatccatca tttgccgttc tgatcggacc gcgtgaaatt acaagcggtc
     2341 tgaccggaaa cccgagggac gttttttgat gctgttgatg tgtcgtataa agcaaacaca
     2401 acaaattgct ctttagatat agttgtgcag gctggttaga aaattcacac ccagataagt
     2461 aaataaaaca gacacgaacc cgacaatcgt catcatggcc atgcgccggc cgcgccagcc
     2521 gcggacaaag cgggcatgca gcataatggc gtagataagc cacgtgatca gggaccatat
     2581 ctctttggga tcccagctcc agtatgagcc ccaggcatag tgcgcccaga cggaaccggt
     2641 gataatcccc atggccagaa aaataaatcc gagtgatgtg ctctgataga tcagaacatc
     2701 cagtgtgttc agcacggaag aattttcctt cggatgagac gcagggttgg tttctcttgt
     2761 ttcttttttt tccgtccaga gataaaggag tccgcagccg aaagcaaccg tgaaggcggc
     2821 ataacccata aagcaggtta aaacatgagt cgtcagccag ttgctttgca gcgcgggaat
     2881 cagcggttcg atgctgtgat cgacccccgg tgcgatggaa gcgtaagcca tgacaaaaaa
     2941 agccaccggg acgacaaacg tgccgagaat tctgcttttg atccgccact caatggccag
     3001 atagagcatt atgatggacc aggcgaagaa aaccagagac tcatacaaat tggtaatcgg
     3061 cacgtgtccg aatcccaggt cgtaggaagc tctccatcgc aacatcatgg ccagcgtgtg
     3121 cgcgaaaagt cccagccacg ccagcaagga tgccgcgtat ccccagaatt cttttttcaa
     3181 aatcattctg aacagataaa aaacaaagga agcaaaataa ataaacgtca cgtaacttaa
     3241 aatcagcgtg tggttcatgc cgccagctcc ttttccatcc atgcgcacaa atcatcaagt
     3301 cgcctctgca acgcttcgtt ataacgatga ctgcgcccgg ccacgcggat tctgacgcct
     3361 gccggatcct gttccagcag aaaccagata cgccgatacg caatgaaaaa gatgaccatc
     3421 atgccggcga tcagaatcaa tccgccggcc agaacaaacg ggacacccgg atcgcggaca
     3481 acctgtaacc cggtgtaata ttttccttca atcccccgga gagaaaaaac caggggtttg
     3541 aaaaggcccg gattaaaaag agggacggcg gaaaataagc cggggtttac atccgcgata
     3601 tcctgaatat gctgaaacat ccagaactgg atatttctct tgtccgtttc aatatttagt
     3661 tttacggccg gaccgaactg catcatattt tcttccacgc gcaaaacggt ggcctttgcc
     3721 ttctgaacgg gaagatcaaa cgtgtctccc tgtcctgctg agatttccgg gctctccgct
     3781 ccggcgttgc tgtatgtcag acgggctctg cttttttcag agacaccgta gcttgcctga
     3841 taaaaacgca ggccgtcaaa cgatagcgga tgattgacca gcacagaacc ctgacgcacg
     3901 acctgtccat ttttgatgaa acttaactcc gatcgatagg tttttgggac tcctgtgtca
     3961 taaaattcca caacaaattt gtcgcatcga acggaaaaat caagcgggtg ttcaccctcg
     4021 ccattccgca gctggataat gtttgtttct tcgccttcgg aaagtctgag atccgcctca
     4081 agaccgaaca ctgagccgat caccatgccc gcaataatca tcaaaacgcc aagatggaca
     4141 acgtaaacgc cgaacaggga taggcgcccc gattcgcgat aaagcatttt ccccttttcc
     4201 agctttgttt ctttaaccga aaatagtttc gatttgagaa gagattcgac cgcgcggctg
     4261 acggtttcca tctttttatc cgtcacgatt atccggtttg gagcaagatt ttcatacagc
     4321 ccggaaggtg gcgggaaaga gggcgtcttg tattgtttta tggcgaaagg aattcgttgg
     4381 atggagcaaa ccatcagatt cagtgagaaa agccccatca acacataaaa cattttggaa
     4441 tgataaaggt cattgagcca gagccattcg gaatccattc tctgcggcgg caccagcgtg
     4501 gccacaataa agatcaccac catgaaaaaa agcaaaacga tggcaagttt gacggaagca
     4561 agaaaagacc agatgaaatt cgcttttttc aatggtttct gcccttaaag aaaagaaaat
     4621 ttttccagaa aacttaacat ctaatgtgct ttctgtcaaa gagtaccttg ctcagacagc
     4681 ccggacgaac ttcttgacaa aacaaccggt ttaccttatc gtttaattgt ctttcagtgt
     4741 tttattgatg tgattgtcgc agcaattcaa caaggaaaat gatatggccg attccggcaa
     4801 aaagcggttc ctcccacata tcaaatctgc ctttgaacgc gtcttatccg gtggatggat
     4861 ttatctcttg tttgtcggcc tgttcggtat ttttcttctc ataggaattg gtgccgggat
     4921 tcatgcgttg tttatcgccg gcagccgtca tgcctacgcg acatcacggg aaattcccgt
     4981 tgccattctg attgccacgt atgttttttt tgttgtcgct tcaacggggc tttgtctggt
     5041 gtcgtcactg ggacatgtct tcggcgtgga aaacttcctg cccctggcga aacgttgtgt
     5101 ttacctggcg atcattacca ttctttccgg attcctggtg atcggcctgg aaattgaaaa
     5161 tcctttccgc atggcgatat acaacgttat ttcgcccaac ttctcttcca atatctggtg
     5221 gatgggaacc ctgtacgggt ttgccgtcgt ttttctgacg attgaattca ttcttctgtt
     5281 taatgaagcg caccaccata aagctgtcat tgccggtttc ctcggcgtca ttacggaagt
     5341 cgccgccaac agcaacctgg gcggtgtttt cgcgatgatg catgggcgcc cttactggta
     5401 cggcccttat ttccccgttt attttatttt atccgccctt attacgggct gcgcttttat
     5461 tctgtttttc ttttttctcg cccacaaagc ggtcaacaaa aaaccattca tggaatcgac
     5521 gcaaaccgct ctgaaagccg tatcgaaact gacgctgctt ttgctggccg tcatgatgat
     5581 cttcaccgcg tggaaggttt tgacgacctc cgttggcagt cctgccgccg ccgccgcgtt
     5641 gagcgtgtta actgaaggac cctattcatt caacttctgg accattgaaa tccttgtcgg
     5701 aatggtcatt ccttttgttt taattctggc atcaagaggc aggaatctga cggtcatgtt
     5761 catcgccgcc gtcatgatga ttttcagcct tttcttcacc cgtctggata tggtggttgt
     5821 cggacagatt gtcccgcaat attttgaagt gggtgttgtg gaatattcca aattgcatac
     5881 ctattctccg agcattcacg aaatccttgt ggtgctggga gggataagtt tctgcctgct
     5941 ggcatttttg ctgggtgaaa aagtattttc cggtttcgca gattcaccgc agaccgacat
     6001 catgaaagaa aaaacaacgg acgttgccgg agtggaagaa ctgacatgac ggataaggcg
     6061 agccataaaa atattccgga atctccgttt gatgctgaac ggcgggcatt tctgaaaatc
     6121 ggtctgaaaa ttaccggcgt gctcgccggc ggctcaatat tgtctttagt ctccaacatc
     6181 aacaaggctt attctcttga tctttttcgg gagaaatacc cttataaacc tcattacgcg
     6241 atggtgttaa gacaggaccg ctgcattgat tgcgaaaaat gcatgaaagc ctgtattgaa
     6301 accaatcatg ttccggatta cggctggcgg acaaagattt taaaacgcga cctttcgtcg
     6361 gcgcttgatc agaaatggga atttattccc atcctctgca atcaatgcaa caatcctccg
     6421 tgcgtgcgca cctgcccgac gacggcgacg tataaggata agaccaacgg catcgtgcag
     6481 atgaacaaca aaaaatgcat cgggtgcaaa tcctgtatta tggcctgccc ctacgatgcc
     6541 cgctattata atgaagaaaa aggtgccatt gataaatgcg atttctgttt tgagtccagg
     6601 ctgtccaaag gcattaatac cccggcctgc gttgaagcct gtccctcgga tgtccgtatt
     6661 ttcggagatt tgtcggatcc gtccagcaga gtgtttcaac ttgttcacca gttgcaaaga
     6721 cctgtgtggg tgctgcggca ggagatcggt gcgaaaccga atatttttta tatgaagggt
     6781 tgatgattca gcaatgaaaa gaaatcttgt tcttataatt cttctcgctc ttttctgcat
     6841 cgccaatgtc ggttctaccg gaaaaagcag tttttccgac ggcggtggca ttctgtatac
     6901 gcagccggtg aaatccgtta ttttccgcca tcagcatcat gtggatgtaa aaaaaataag
     6961 ttgtgagaaa tgccacagcg gcttgtttga aatgcaggct ttgcttgccc aggagaaaaa
     7021 ggattttatt atggattctc tctacaaggg caggtactgc ggcgcgtgtc ataacggcaa
     7081 agacgctttt gcatccgata aacagtgcgc cagatgccat gtccgcatca gcgaaaaaga
     7141 tgtggggcac gtgaaaggta aagccaagcc atacaaagcg cctgtttaca gtacggtggc
     7201 cactttggga aaagaccagc ggaaggtccc gttcaaccac gaaaagcatg cgtcgctgaa
     7261 ttgcatggat tgtcattcga aactttttca aatcagaaaa ggatcaacca agattgccct
     7321 ggaaacgcat cagtcgcaaa aatattgctt taactgtcac aacgggaaaa aagagttttc
     7381 ctggaacaat tgtacaaaat gccatcagaa ctggaaggat attaaaccgt acggtattct
     7441 gacggcaaga gccgacaggg gaagctgtgt tgcctgccac accaatccgg atgaaatgaa
     7501 atcgctcgtc aagccgcccg aaataggcgg cgaggccgag gggtgagctg gcgctcctcc
     7561 cccggtccgg gcggaagaat actacaaagg ttatctggtc gataaaggaa tgatcggcaa
     7621 ggagttccat ctttcgatgg attgcattgc ctgtcatcaa ggcaacaagc tcgcaaaaac
     7681 aaaggagaaa gctcatcaag gacgtatcgc cagaccgtcc gacaatctta aaacctgcgg
     7741 catctgtcat ataacagccg gacaaaatta cgccaaggcc cttcattata cgagcctggg
     7801 ccagcgcgaa ggcgtcaaac accggttttc tgcggcagaa ttgaaaacgt ttgatcagaa
     7861 agtttttgaa aaatcatgcc gatcctgtca cgcttcctgc ggaagctgcc acgtcaaatc
     7921 gccgcctgtc ggcggcatca ccaccggcct tctccagggc cataaatttg tgaagaaaaa
     7981 cgaaggcaaa acctgtgctt catgtcacgg tggaagagta tatcccgaat ttaccggtga
     8041 atacggcggc acccctgatg tccactacca gaagggcatg atgtgcaatg actgccacaa
     8101 caaacaggaa atgcacggcg acggcaaatt acataaatca aaaaatgatg tcaaagaccg
     8161 cccgaaatgc ctgtcctgcc atcaggataa aggcaatcag gtcgcgcaca ccattcataa
     8221 ggataaagtc agctgtcagg gctgccattc ttccggccaa tatcgccagt gttatagctg
     8281 tcatgccgtg catgggtcaa aggccgagcc cggctttatc ctggggctga atccgcgtga
     8341 ccgcaagacc ttgacaaccc tcagggtcat acctactata agaagtacat ttgagcctaa
     8401 cggaataaaa atggagaatt ttgatgctct gcccaattac tggaattcgc cggtgcacaa
     8461 cattaaaaag cgaacaaaca ggacacgctc ctgcgatgca tgccatgtga ataaagaagg
     8521 tttcctgaaa cctgaagggc tgattaaaga cggatctaaa gaaaatgaga aactaattta
     8581 tgacattaaa ccaataagca aataaaaaca ggaggatctt tatgaaaaga atgaggtttt
     8641 tgaaagtgtt gttggtatcc ctgttgattt tactgccggc agccgtgctg gcgcagggac
     8701 tcagccctat tgtttcgaca gatgatttag ccaaaattgt aaaggatccc aacgtcattg
     8761 tcattgatat ccgcaaggtt gaagattata aagcggggca tattcccggc gcgatagggg
     8821 tgttttataa taattgggca ccgggcatgg gcggcttaaa aaatgaaatg ccggcagatg
     8881 atgatttaat ggaccttctt accgataacg gcattcaacc cgactcaacg gtcgtcgttt
     8941 acggcactac aggaactccg cctgatcgtg tggatgtgac ccgcgtggcc tgggctttga
     9001 aatacgcagg cgtccagaag gtatctgtac tgagcggcgg atttgagaaa tgggcggtgc
     9061 gcgagaaaaa agaagtttct acagagatta ccaagcccaa gaccaaagat tacaaaggta
     9121 aaatcaataa agccattctg gttaacaagg attacgtgaa aaatcacgtc ggaaaagcga
     9181 tgatcgttga cgcacgggaa ccggcattct ttaaaggcga acagaaactt cctttcgtgg
     9241 ccaaagccgg tcgagtcaag aattcggtca atctgcccac cattcaggtt ttcgacaaat
     9301 atccccccgg cgagcatatg gagccctgct gctggacata taaagatgtc gccacgctca
     9361 aaaacattgc ttcaggtgtc gttggcagtg acatcgacaa ggaaatcatt gtctattgcg
     9421 acaccgggaa agttgccagt gcctggtggt tcattttaag tgatgtgatg ggtttcaaaa
     9481 atgtgaaaat ttatgacggt tccatggaag aatggatgaa tgatgcaagc gctccgacgg
     9541 aaccgtaaag cataaagaac atcatgaaaa aaagcagccc catcgtttta tggggctgct
     9601 tttctattga acgttaaaca gactttctat ttaggcgtga cgttggcgtc ggcgtttttt
     9661 gtaatcggtg cctgaggatc ctgcatccat tcctgcatgg cgccatcgta tagacggacg
     9721 tttttatagc cgaggacttc caccagaata taagaccaga tcgggcagca ctgaccgatg
     9781 tcgcaatagg ttataatttc tttttcctta tccctgccga tagcggcttc ggaaatcgcc
     9841 gcgagttctt ccgtggtttt aaatgttttg tcaggattaa aggcgcagga agtcggcatg
     9901 ttaaaagctc ccgggattcg tcccatcatg ggaatacaat ccatcttttt ctcgccggta
     9961 aagaactctc gttctctcac atccagcata atagccttgc ccagccgttc tttaacgtaa
    10021 tctttgtcgg caaatctcgc tcttgagtag aggcctttga atggttttgc ttttgcgtgc
    10081 atgatctcct gcgaaagcgg tttcttttca gtaatccatt tattcatccc gccgttaaga
    10141 acggctacat tttttattcc tgcgtattgc agggtgcagg cgacacgggc gctttgataa
    10201 ctcagccgcg gcgtatccat gcaaccggtg acgacaatcc gcgagtcaaa cccgattccg
    10261 aagtagccta tggtatcatc caggtcatcc gtttcgggga tctccgtaaa aagaccgtct
    10321 ttctggtatg cccatgatct gtaaaacgcg tttagcgcgc cgggaatgtg tccctctcgg
    10381 taatcctcaa ctcggcgaac atccaggata atcaatttcg gattcttcaa atttgccaaa
    10441 agccagtcgg tggatacaat cggctcgatg tcacgggttt gaccggctat cggcaataaa
    10501 aggataaaaa gaaggatcgt aaggagcttg gacaaagcat gatttttcat atggcaatct
    10561 cctttgaatg ttgtattaca catcttaaaa cggatgtata catagtatac cactaattct
    10621 ttattgagac agccgaatat ttcagaccgc agaatacaag ccctatttca tactattttt
    10681 atcttaccga atatgacggt ctatttcccc catcagcgtc tgcgtctgat cgggcaacag
    10741 cgcgaatccc atgtcattcc tgtaaatgat gttcattttt ttatcgacaa tcacatacac
    10801 gcgtttggaa aggggcaaaa gccagtggtc ggcgtcgtag agcttggcga ttttgccttt
    10861 ttcatccacc agcagagtca tgtttttcag gctgtgcttg gctataaact tgcggtgcga
    10921 ttcggccgaa tcccgcgaca ggccgagaat tttgattttt ttggctttgt attttttaat
    10981 attttgttga aactcaacaa actggcggat tcaaagcttg gtgtcgtctt tgggatagaa
    11041 agccagtacc acggcgcgat gatttttgac agcattgctg agtttccata tgttgccctt
    11101 ttgatcggac aatgtaaaat caggggctac ctggccggaa accggggagg cgttttttgc
    11161 ccctgttgaa gcacaggctg acaatataaa gaacaccagc acaaaaaaaa cgaattttgt
    11221 tattcgcata tagccctccg ataaaggtat ggacggcggg cggttcaaaa ataaccgccc
    11281 gcccatctta gtaaagatta atcgtcaaac gccattttgg ttttccaaac ttcccagacc
    11341 ttgcctacca gatcggggcc gggtttgagc gtcggcttgc cgggtttcca accggagggc
    11401 gtggcttcgg cgcctttggt tttgcgcacg tgttggaagg cctgaatttg acggatgctt
    11461 tccatgacgt tgcgtccgac cggcggcgtc aacacttcaa agccctgaat gatgccatcg
    11521 gggtcgatga tgaaccggcc gcgggcttcc acgccggcat cctgaaggta aacgccaaag
    11581 acgtctccga ccttgccgcc gccgtcggag agcatgggga aggggatgcc gcctttgacc
    11641 attttggaaa gttcatgatc atcccacatt ttgtggacaa agacgctgtc cacactcatg
    11701 gataaaactt caacgccgag ttttttgaat tcgggatatt tctcgccgac cgacgagatt
    11761 tcggttgctc agacgaaggt gaagtcgccc ggataaaaac acaggacaac ccatttcccc
    11821 agataatcgg agagcttcac attgatgaat ttccccttgt gatacgcggg cgccgtgaag
    11881 tccggcgctt ttttccctac agtgaccatg cttttgacct ccttgacagg tgttggttga
    11941 gacgtttctt tcttcgcatc cggtttttcg ccgaccggtc cgccggtggg tctggcacag
    12001 ccggccttga tttcttcagc catagatgat tccctccttt gttttatttt tttgatttct
    12061 tcattttcgc gatttcttcc tccacttcct cagcgccttc cgcatagaat tttccttcat
    12121 cggggccagt tcatgaagca atcttaaaaa ctctccggcg tgaacgcgtt cctcgtcggc
    12181 aatgtccccg aggacctcga tggccagctt gttgtcggtt gattccgcga gctgcatata
    12241 aagttggatg gcttcatatt ctgccgccac cataaagcga atggctctga tcagttcttc
    12301 tttggtcagt ttcctgtcct tcttcaaccc tgaaaaaggt gttccaaact ccggcatggt
    12361 atcatccccc tttcctgtta ttattatagg ttaatttcct gatgcagtaa cgataattta
    12421 tgacatcatt ttgtgatgtc aatttctgcc cttatttttt tgatatttac ttctccctcg
    12481 ggaacggcca agccataatg ccgccttcca tcactttgac gtttttccag ccgttgcctt
    12541 ccagtgccag cgccgcctca taaccgcgca gtgaaatctt gcagtataag atgatttcgc
    12601 gatttttatc ctccggcagc tccttgaggc gcttacggat cgcgccgagc ggtatcaacg
    12661 tctcaccgat gcctaaacgc atctcttcga attcctccgg tccgcgtgta tcaatcagga
    12721 atggtttctc tccggcccgt attttatcat aaacctcttt acaactgata cctttcattc
    12781 tgcctttcat tttattctgc atgatgtgcg ccgtggcgat aaaatgatca atggccagag
    12841 agaagggcgg cgcgtaggga agatcggcgt taatcaggtc ttccacggtc attttcccct
    12901 gaatggccat ggcccactgg gcaatttgct tggcgacgtt tcccggaccg atgcattgtg
    12961 cgccgaggat acggccggtt ttttgctccg ccaccagttt agtgaccaga agcttgcctt
    13021 ccataaagcc cggtttgtcg ggactggcgt tgatgaccgt tatgatgtcg gaatagccca
    13081 ggcgtttggc cattgtttcc gaaaggccgg tggagcccgc cccgtaatcg aacactttgc
    13141 agatgccggt ttgaatcgtt cccgggaaag tgacgctgtt gccggaagcg gcgttttctc
    13201 ccgctattct tccctggaga ttggcaagat cgccgtaagg cgcgtgaact tttttgccgg
    13261 tgatgcggtt gagggtttcc acgcagtcgc ccacggcgta aatgtccggg tcggaggtct
    13321 gcatgtattc attgacttca acgccgccaa gctcgccgat tttcaacccg gcttctttgg
    13381 ccagtgtgac gttgggacgg acaccgatgg ctataacggc cagctcgcag ggaagctccg
    13441 ttccgttttg cagtttcacc gctgtgagtt ttccgttttc acccaggaaa gcggctatgc
    13501 cgttttcggt gatgatgttg gcgcctttgc tccgcacatg gttttccacc agcttggcca
    13561 gttcccagtc caaaaacgtc agcagttgag gaagcagttc aatgacggtg atttcaatac
    13621 ccgccagttg cagcgcttcg caggtttcga taccgatgag tccgccgccg atgaccacgg
    13681 cctttttgat tttgccttca tcgcggacgt tacgcagaaa atcggcgtcc ttcatggatt
    13741 gtaatgtggt aatgccctgg agatctgtgc ccggaaccgg cggcattcgg ggaacggcgc
    13801 cggtggcgat aacgagtttg tcatacgtca gggtgccggt ttctccggaa accgtatttt
    13861 tgaaggaaac ggaacggttt ttcgtgtcga tgttagtgac ttcggtgttg accttcgctt
    13921 cgatatcttt ggcgttgagg taaaacatcg gattgcgcac gacgcccgcg ggcgtgcaaa
    13981 gtagcgcgtt gcggtcgtta aaaaaacctc cgacgtaata cgggtagccg caggacgcca
    14041 tggaaaggtc ggcttccttc tggataatga tcacttccgc gtcgttgtca atgcgccggg
    14101 ctttcgccgc tgctttggga ccggcagccg aacctccgat aacaatgatt cgttttctct
    14161 gagccatagt tttatcctcc gatttttaaa agtgacctga acataaaagg tcgcttccat
    14221 aaatttgatt ctctcagtga aaaaatagca attatcggca gccgttgcat tgacttaaat
    14281 caatgcggat aattattttc agagtccgta tattttgagc cggccggcat cagaccttat
    14341 ggaacataca tggcaacgac ctcatccgca gcataaatta ccccgccggc gattagctgt
    14401 gatttaataa taacttttac gatgatgcgg gcgatatttc agcatcaacg tgcctcaacg
    14461 gaagcgtggg atgttttatg aaagccctga tgaaaagggg aatccttcgg gataacggtt
    14521 tggtgatcgt atccccgtag gctttcagca actcatccgg catgtagtga agctttattc
    14581 ccgcaattct gttggtgaag gagagcgtga taaaacatcc ggggcagttg acggccatat
    14641 ctttcattcc ggtttccttc acttctttgt attttttcca ctgttccttg aagagacttt
    14701 tcggaagatt aagggtccgg agaacgctca cggccccgca ggaaaggttg cactctccat
    14761 gatgggggag ttccactata tcggcgcccg ctgatttgta aacggcgcga aggtcgcggg
    14821 caaagccgtc acccagttcg ctgacataac atgattcatg gagggtcgtc tttagtttca
    14881 acggttgttt tactgtgatc ttgccggatt ctcttttttc attcagccat tcatacaggg
    14941 aaatcaattt gaatggcaat tccctgccgt atacattttt cagtatattg gatagatagt
    15001 tgtagcagga tccgcagtag caaaccattc tctcgatatt caatttctca aaagctttca
    15061 gggttttttc aatcgtttcg gtgtaggctt tccatgaacc gagacggtat gcaagctcac
    15121 cacaacagag atccggcggc ccgaatttcg gcagttcctg taaaacagcg gagttttcaa
    15181 tatcgaaaca acttattctg ccgatgcatc cgacccacag aatttctttt gaaggcggtg
    15241 gaatttccga ccattttttc aatatggatt gttcctccct ggaaagcatg gcataaatat
    15301 ccttccacat tgacattttc atgccgttga agagataggg gtagacagcg gatatctttc
    15361 ctttcttctt cctgttctcc atgaacttct ccagaataaa ttcatgaagt cttaacccct
    15421 cggggcaata attattgcaa ctgaagcaga acgtgcattc cctgaaaatt ttttccgagt
    15481 aaccggtttt caataatccg gtcatttcag caacggcctc gggcttgtcc atgtgcatca
    15541 cgggacattg gacaagacat tcaccgcacg tcgtacattt attccaatcc gataatttgg
    15601 cgtatgacat aatacatgtt cccccttttt atctaaattg acgagcaacc cattcaagtt
    15661 tctggatacg cgccgcatgc catgcaaaaa atttttgcca gagcccggcg ctttttatct
    15721 catgcgtcga atgcgtgttt cgatcttctt tgtgtgagtg taattctttt aataacggca
    15781 gtaaatgcag attattgctc cgttcatgga taatgtgcca tacgcaattt atttgcggat
    15841 cgagcgcgca tttcaagttt gattctgaat aggatttgca ggggccgtat ttgctttttg
    15901 cgggacattc gctcaacgga cagattccag cggtgcaggt tagcacgcaa tgtccgcagc
    15961 ttctgcagag gctatgtgca ataaccggat cgctgctgga aactgcggcg cttcccacgg
    16021 aatccagcac attgattaca ggcaaatccg gccgacagct attcgctgat tttattcccg
    16081 ccgcgcaact caaaacgatc aatgcatcac acttttcgag ggcctttttg ttggccctca
    16141 acgcctgacg cattatttct tcgggacaag cggcaagaat gactttgtcc agtttgatct
    16201 gtttgtgatt tttcagcaga tgcgctttca gaacttccac gccggtcttg ccgcccgtgt
    16261 aggaaagatt ggcgcatgtt ccacaactca gaatgccgaa tgttttataa tcttttagta
    16321 tgagattaat ttccggctcc ggtttccaaa tggttttatt cattgtttac cgtatcctga
    16381 ctgtgaaaag cgtattgtat atcggggctt gggatctgct attcttttaa atggtccggc
    16441 atcagggctt ccggcacctg aatggcaacg acttcgccgg tggcgcatat ctggccgccg
    16501 gcaatcagcc tcgattcgat aacgactttg cgccttttga tctccttgac tttcccccgt
    16561 atttctatcg gtccgccaag cggtgtgggc aaaagatagt ttacgtgcaa agaggcggtc
    16621 agaaaacgaa tcggcggcag ggtatccatc tcgcggtttt cagagcagta tttggccgcc
    16681 gccgctgtgc cagtggaatg gcaatcgatc agcgacgcaa tcagaccgcc gtaagccatt
    16741 ccgggaatag ccatgtagcg gggatccggt tgataaacac agacggattc ttctccgtcc
    16801 cagtagcttt tgatttgcag accgtgttcg ttgaggcgac cgcagccgta acagtggctt
    16861 aaatcgtcgg gatagtagtc ctgaaacgct ttttgcttca taaaaacccc ttttttatac
    16921 aagaaatttt atatttacaa tcgagaggac agaaaagcat gcattttttg cgcgtctgtt
    16981 gcaatatcaa catgcacgtg ttcattgtca aagatgacat tggttgaact tctgaaatta
    17041 tacggcaatg cctcgacggc atcgattgtg taaattttta catccagaat ggtctcaaat
    17101 tttgttttca tctcttcagc tacacctata gccttcttgc agcttattca gcccggattt
    17161 ccgctatgaa aaaccagtaa tgtcttcatg atagtcccct ttatgttaat taaatgtcaa
    17221 ttttgttccg gccatattca aaataaattg ttcgggcatt tgcttttcca tctccataat
    17281 ggctttgcgt cccgtgcagt gtgtcggaat gatataagcc gggttgattt tcttgaattc
    17341 ctgcgtggtg cgatcaataa tcggttcaaa aaacggcccg gatagatgga atccgcccat
    17401 cactgcgtga attttattaa taccggtaac tttcatcgcg tgatgaaccg tattaatgat
    17461 tccggaatgc gcacaaccgg aaaggataat caaccctttg tttttcagat tcatgacaat
    17521 agaggtgtcg tcttcgatgg catcccaaac ttcctttccg tcttgctggc aatgaggaag
    17581 cggccatccc gtttcgaaat ccgtgctacg gggaatttcg cccagaaaaa gaatcgtatc
    17641 ttccagtaaa agatatggtt tttcagtttc caccacggtg agacccgcgg ctttaaccat
    17701 ttcccgcgtc aattccggaa aattgatttt taattcctct ccgaatttga gataacgcgg
    17761 cgatttaaac gcggacggat gcacggcgaa agggatattc tttttaccgg tcatggtgac
    17821 gagttttgcc atgccacccg tatggtcact atgtccgtgg gaaagagcgg ctgcctcgac
    17881 ctgtgtcata tcaacgttaa gcgtttccgc attttgcgcc gcaccgtttt ccgaaaaacc
    17941 gaagtcaagc agcagcgtat gcgttttgtt tccgagagtt gtttggacga gtgcggaaaa
    18001 accgtgctca gccaacacgg aagcccggat ttctccttct tttaaaggat tggctcgcgt
    18061 gacaacagcg ttgctgtcca tcgcggtaat ttcaatgtag ttatcctgta atgtcaggat
    18121 ttcaacccta tcaactgctt ttaaagctgt tgtcatgata ccaatcctcc tttgatttag
    18181 tgatcgcaga gattgtctcc gacttgcaat gaaccggcaa aaaaatccgt aatcagtttt
    18241 tccggtgttt cgaccggagc accgactacg acctgaatgc cctgctcggc aaacaactgt
    18301 tgggctcgtt gacccatgcc gccggcaata atcatattag caccttgttt ggccagccat
    18361 ggcggcaaaa gtcccggttc atgaggcggc gcggcaatgt cttcacgttt gatgattttt
    18421 ttagtttggt cttcgatttc gatgatagca aaactcgcgc agtgaccgaa atgcgctgta
    18481 agttttcctt cggctaacgg tatggctatt ttcatgattc attctcctta tgagtttaat
    18541 atttttgtct tatccaagtc ttttgtaatt tcatcaatga ttttttgcat gatttcccct
    18601 gtgggcgttt tcgatgaatg ataaataaaa accttacccg agtcgccgga ctgtgcgatt
    18661 gccggatcca tgggaatagc gccaagaaaa ggcacattca tgtccgttga gattttcttg
    18721 ccgccgccgg tgggcagaat ctgtgtaatt tcaccgcatt tcggacacgc aaaaccgctc
    18781 atgttttcga tgacgccgac caccggtacg tcaagacgct tgcaaaagct gatagatttg
    18841 cgcacgtcca ctgcggcgac tttctgcggt gtggtgacaa taattgcgcc gtccagcggc
    18901 tgaatcaact ggcacaacga caggggttca tcacctgtgc ccggaggagc gtcaacaatt
    18961 aagtaatcca gatcgcccca atgcacatcc gtcaggaatt gtttgataac acccattttc
    19021 atcggaccgc gccagatgac agcgtcatcg ggattctgca acaaaaaccc cagagacatt
    19081 attttcaggc caccgaattc aaccggaaac aatccgtcgg ggccgccttt gagagtttca
    19141 ttttccagtc ccagcatggt gggtacgctg ggaccgtgaa tatccacgtc cagcaaaccg
    19201 actcgcagac cgtttaatgc cagcgcggtg gccagattga ccgcaacggt gcttttaccg
    19261 acgccgccct tgccggaaag cacgacgatt tttttctgaa tgcggcagag cgtcgcctgc
    19321 aatttgcggc gatcagcgta ttcttcagga ctttcatcct ttttctgttt tttagccgcg
    19381 cagtcggaat cgccgcacgt atcacatgtt tttttttctt ccactattat atcctccatt
    19441 tattttattg attaagataa tcttcacaag ccgcttttaa cgtattggtc gccagaagcg
    19501 cgcagtgttc gtgatcttcg ggaagtccgt caagaacatt gagaacatcc ttttgactta
    19561 ttgcttgggc ttcctcaatc gattttccct cggccataca ggttgtcatg ctgccgcagg
    19621 cacgcgaagg atcacagcca tcggtgataa aagagacttt cctgattttg ccttcttcaa
    19681 ccagaatcca gaactccatg gtgtcgccgc agggtccggt aattctcgcg tgtccgctaa
    19741 aagaatccat cggtcccata ttatgaggcg aatcggcatg tttttttgcc gtggcataag
    19801 tttcgtccag atttactaac tcactcatta tttaccttct tatacctccc gaagattaag
    19861 atttttccag agttgttgaa tatcatcgcc gcttggggca tcgatttcaa caaccgattt
    19921 tttctgtatt tgcgcctgtg tgacggcatt gtcgtagcga atccgtccgg caattcgcgc
    19981 gcctaatccc gcagcatttt cttcaatacg ctcagtcatg tcgggattga tatcccattt
    20041 gttgacgcac acagctgctg gaatttgaaa atgacgggta aggcccagca ccctttccag
    20101 atcgtgttcg cccgaaactg ttggttccgt gaccaccaga acctgcgaag caccggtcac
    20161 cgaggcaata accggacagc cgatgccggg aggaccatcc acaataatca attcacaatt
    20221 ttctttttcc gcgatggcgc gggcttcccg ccgcacggtg gaaaccaatt tgccggagtt
    20281 ttcagcggcc acccccagac gcgcgtgaac catcggcccc agacgcgtat cggagacaaa
    20341 ccacttgccg cagttgcaat ccgggaaatc aatcgctttt tccgggcaca tttccacgca
    20401 aactttgcag ccctcacagg caacgggatc cacggcaaat aaaagctctc cctgaaaatt
    20461 ctgcttactg tgaatggcat taaagcggca aacgtcttta caaataccgc agctgataca
    20521 gtcggtttga cggataacag cttcatgacc gctgataaat tcgtgttctt ccttgatttg
    20581 cggtgtcagg actaaatgca agtcggccgc atccacatcg caatcacaga tcaccgaacg
    20641 ctttgccagc gctaccagtg atgccgtaac gcttgttttc cccgtacctc cttttccgct
    20701 gattattacc aattccttca tggccatcat tcctcaatat ccggcgatag gtttgttctg
    20761 ccggccacaa gcatgtcctg caagtcgctt tgatgttctc ttacagtgaa tccgcgttcg
    20821 accaggatgg tcttcatctc cgccagggtg ccgccgctga cgtcgtggta tcttccttcg
    20881 cgctcgtgaa tcctgtccaa tatcgcgagt cccttttcgt taaaatcgct caacacgatt
    20941 ttaccatccg gcgatagaag tcgtgtgaat tcatcacaga ccttgcggat agaggaaagg
    21001 tgatgaatca tgttcacggc gaaaatcaca tcaaaaaaac cgtcgttaca ggggatggat
    21061 tcaacatcgg cgatgaggaa agtcacctgc gactgaaatc cataatacat caggttgagc
    21121 agggcgaatc tctgttccgc tacggaaata tccaaagaga aaaatttata tcctgcctgc
    21181 gccagagcaa gcgtgaaata gcccttgccc gtaccggctt ccagaatgtt gcccgctaca
    21241 ggcaacgatt tttcaatgat aaaagaacgc tccagatcga tattgtatcc gaatgtccgg
    21301 taaagcgcga cacgtttctg atattttatg tgattttcga taatgtcctt ttgcatcgtc
    21361 aatccttcat cttattttct ttccgctatc ttgtaaaaca agccttgttc ggcgcgcatt
    21421 gcaatgatga gccgcttatc taaaagactt ttcagctttt tggccacgtc gtgcggatgc
    21481 aatcccatcc cggtactgat tccctcgagt gtgcatggtc ttctggccag aagatccaga
    21541 atgtctttgt ctgaggattc caccccattt attccggaaa gatcagagga tgttgcgttt
    21601 tggagaatgt cgaccggaca taaaaacaaa cgggcaagtt tctgcaattg tctgacgtca
    21661 acgggatggg caaaatcttc ataggctgga cggctcacgg tattaatttg aactttttcg
    21721 atatgaattt tattgaccca tgccgccatt tcttttactt cttccgccat accgttaatt
    21781 cccgaaagca ggagaatttc cagccagatg tgtcctgtaa attgttttga aaaatcaatc
    21841 agcccctgaa tcactcgttc gaaagaaata tctttgtgcg gacgattgac gtattggaaa
    21901 agttgctcat tgccggcatc cagcgaagga atgacgatat ccgccttcat caatgattgg
    21961 cggacctccg gcagataaag cagagacccg ttggtcagaa cggctaccgg aatgttcgtc
    22021 ctttctttta tcttaccgat gatttcaccg atggatgcgt gcagggttgg ttctcccgaa
    22081 ccggcaacgg tgatgtaatt gcattcaaac ttcgttaata acttttcttc gatttccgac
    22141 aacacatcag ccaaaggaac ataatcgagc cttacggtcg ttttatttgt cgtttttccc
    22201 agttgacagt agatacaatc atacgtgcag gttttatacg gcacgatgtc cactcccagc
    22261 gactttccta acctgcggga cgggacagga ccatagacat attgatagac tctttctgac
    22321 attttaatct ccacaagggg cttgaccgca tcgttgcttt gcaccgcaac aggttgttcc
    22381 cgtttcttca aaggaacagc tttttgcagt gtcgcgcata ctgacaccgg ctgacttcat
    22441 aataaaacca ctgccgccgc taacgattct ttgtacgtca gctctacatt cgggacatat
    22501 cctgataggc gcatctttca tgctttgttg cctttcaaaa cgtagaccgc acttgctgca
    22561 ttcatattca taagtcggca tttgttctta tcctttcttt ttcgaattgg caattttctc
    22621 taaaagattt tcaaaacgtt cccggtattg aggcagggca tccaccatca gaattccccg
    22681 tgaataattt tccgcaattt gccggtcatc gggaatttcc atcagaatgg gaacgttttc
    22741 ctttcgacag aattcatgaa cgcggttatc accgctcccc attcgattga caagaacgcc
    22801 aaagggcaga ttcaactctc tgaccatatc caccgccagc gataaatcgt gcaaaccgaa
    22861 aggcgtcggt tccgttacca gtagaacaaa atccgaatcc ctgatcgcgg caataaccgg
    22921 acaggatgtg ccgggcgggg catctaaaat agccggagca tcaaccggca acgtattttt
    22981 tacggcgcgg ataagcggtg gagccaacgc cacgccaaca tttagttttc ccgtaaccag
    23041 cgtgatattc tccgtgcgcg acgtttcaac aacaccgata cgcctgtttt tttcactgat
    23101 ggccttagtg ggacagacca gcgtgcaccc gccgcagccg tggcacattt ccggaaacac
    23161 aagcggcgtt gttccaaaag tgacaatggc ggagaactgg cagattttcg cacactcacc
    23221 gcagccgttg cagagttctt cattgacatc cggcacgggg aggccgatgg ttttttcaga
    23281 atgccccgat gtttttaaaa ataagtgggc attgggttct tccacgtcgc agtccgcaag
    23341 acacacatct ctcttaaaga cacgcgccag attgacgctg accgtggttt ttccggtccc
    23401 tccttttcct gatgcaacgg ccagcttcat aggattttac tctcctttgg gttgatccag
    23461 agcagaggga aaacgatatc gctggtgcag cagggaaccc ataccgccag aaataaaaaa
    23521 aacgcgatca gtatggtggt aactacatca acggacgtac ccaacgccat taaaacgtga
    23581 aataaggaag cccatgtact cagaagcaca tgtcccgcat ggggaattct ggtatgcgat
    23641 atgacaaacc ctaaagcgat gccggccagc gccagagggt tcaccagcca ccatttttca
    23701 atgaagccca gatgaacgcc ccgatgcggg agttccagca gccattcccc gatgtaggga
    23761 ataaacgaat cgctgaatgt ggcaatgccg attgagccga tataaccgat tatgatgatt
    23821 tgcaagatat tgccgcgcga atgcattcta tacatggcag tggtaaccag ggcgctcagc
    23881 aaaacgtgta acggatgcat tgtccagaaa agcccggagg aaaactcccg cggcacctga
    23941 aactggatga atgcagccat aatgacgatc ccggttactg taccgaacat tgtgaacggt
    24001 gcatgcaatt taagctcttg cgcaatcatg cccgctttca tagggcaact cctgaagaga
    24061 aaccagatat gacaaaacag tttgtggccg cgttcaatcc aatgaaaaat cctgcatgac
    24121 ggcttttggc aaaatattcg gtctgacgaa caaaacaggt tatgccttcc gacggcattt
    24181 tcatgaacgg taccctcgtt ttcccctttt tgctgctgtg gattcatcca cctcatcctg
    24241 ccagccgttt ttccggatat catgcagatc gaattttgcc gtatagggct tgatgtccaa
    24301 aagcggagtt ccgtcgagaa tgtcaattcc atccacataa aggatattgt cctctatacg
    24361 gatgagttct acaacgctaa gcccaagagg attgggacga cccggcgcgc gtgtggaaaa
    24421 tattccccgc tccacgtcct gcagaaacgg tttgactttc agttgaacaa gcggcgcctg
    24481 atgaaaatga tagatcaggt aaacgtggga gaagccgtcc agatcgctta atccagatat
    24541 atatttttca aacacttcaa tgcgtccctt gcaaccgcaa gcgtaaaccg gctgaatcgg
    24601 tgttttttcg gcaaccgtgt gttcactgtg aatgattcca atcggatgat atatgatttg
    24661 tcggtttatc atacccagtg tccatccaca tcggccgatt tggcctcggt taacgtgcct
    24721 tcacgatatt gttccagagc ctcgctgacc gtagcggcgg atgtattgta tatcctgatg
    24781 cctgccgcct gaagcgtccg gaaggctttg ggcccgcagt gtcctgtaat cagcgctttt
    24841 acgccaaggc gggcgatgtt ttgtgccgat tgaatgccgg ctccctgcgc cgcgttaaga
    24901 ttctgttcat tatcaacaat ttcgaaggta ttcgcagcct gatcatagat tagaaatttc
    24961 ggcgcccgtc cgaatctgct gtccagagga gcattcaagt ctacgcccga tgttgtcaat
    25021 gcaattttca ttaaaaaaat tctcctttga taactttttc atagagagca gtgacggcgc
    25081 taagcggtga cttgcgcgcc atcactgcaa tgcatagata agctctatgc gggcttcgca
    25141 aaagaggcta attattttgc gtatctgtct ttagctcgtc caaccgtttt ttaatggcgt
    25201 ctaattccct ctgcagaaat tcagcctgcg ttctgagagc ctgttgttcc tcatggggat
    25261 caggagggac aaccgcggcg gagtattcat cggttcgtcg ccatcccggt attgcagcgg
    25321 cataaaaacg gttgccaaag ccgaatccac cgccacggcc acctcggccg ccaaaaccgg
    25381 cgccacgtcc gaaacctgct ccaaaacgtc ttgccggaat atgattcata taaccgggcg
    25441 ctccgaaacc tgcgcaatat ccggctcctc tacctgtcat cggtcccatt ccaatggggc
    25501 ctgttgcatc tcctcttggc ataatgaaga cctccttttt ttagttattc ccgaatcatg
    25561 gttgaaccgc actgcgggca cttgacttgc gtgcagggaa ttcccctatc atgcggttga
    25621 cggttaccgc atttcgggca tagacagttg cccaaagctc cgcccgcctt catccctccc
    25681 atgcgtcctc ggccaccgcc gccgcgtcct cggcccatac cggaaccgga accttggctc
    25741 ataccgccgc ccgttcctct tggatttcta ttatttgtat tcatgatgtt ttctcccttt
    25801 tggcgtctct tttttagaaa acgaaaactg ccgccgccag taccgtggta tagtcttttg
    25861 ttttcaaaac cgtcgattgc gttgtatttc tcgttccgac gattttaccg ctgattttaa
    25921 acagttcctt tttctcgtcc cagctttcat caatgttgaa atcaatcccc agcgtggatg
    25981 ccagcatcgc tgcggccaaa tcttccgcat agtctccggt ctgtttttcg ttataaccga
    26041 acgaatgatg ctcgctgata taaccgtacg ttttcttatc catgggaatc gcacagccga
    26101 cggacgccga aatcaaacgg cgcggctcgt tgctgcagca gcggctcata acgcagaagg
    26161 tgatggcgcc gggtctgagc tttttcagtc cttcggtgcg tgaaataatg cggcagcccg
    26221 gcggaagaat gctcgaaacc tgaacaaggt tgcatttttc aatgcccgcg tcacgcagcg
    26281 ccagctcaaa agaatgaagc tgttcgcgat gcgtgcccac gcctttggta aagaatattt
    26341 ctttggggat gtactggttc actgtcttgt ctccttttct gcaatgatta ttttcgggtc
    26401 tttatctgct gcggtaacgc ataccgcgct gtttgcaaaa tcctgcgccc atcgctcttc
    26461 tccggcagcc gggcaaagag aacacaggca atcccagcgt gttgttccgg taggcggtta
    26521 taatggcatg gacctctccc ctgacaaagg gatgaacgat gattccctgg gccataatca
    26581 acgcctgcat aggacgggaa atggcgccgc aaattagaac atcgacgccc cgatttttta
    26641 gttcagccgc acgcccggca ggatccgtcg cgacgaattt ctgcaatgtt cttttatcag
    26701 gattcgccgt gtctgcttca acaattaaaa gttgctccgc ggaatcgaaa accgtggaaa
    26761 tgcaatcctt aaataccgac agtgccagct tcattaatgc tccttaggat gaaataccta
    26821 tagaacattc atagcaaaat caatgccaga aggtgttttt aaaatttagt gtttgattac
    26881 aggttattgg aaatgacggt tattcgcttc catgaaaaat aagtagcgtt aatgcaacga
    26941 tagttgcaaa aagtcgcagg aatgcgacta cagagggaat tgccccggaa gggtaatgcc
    27001 gagtgctttg atttttctgt aaagggtgct tttatgaatg cccagttgcc ttgccgtttc
    27061 ccggcaattc cagttgtttt tttgaagagc gttgatgagc caggccgctt ccacgtcacg
    27121 aagagaggac gcatcggttc tgattttctg gggcggagac gcaaaaagag attccggtag
    27181 atgctttttt tcaatccggc cttctttgca gaagatgaag gcgcgttcga taatgttggc
    27241 cagttcccgg atattgcccg ggtaagtgta agccatcaga acggacagtg cttcctgact
    27301 gacgccttca atctcttttt tttgcagaag atcaaagcgg ctgataaagt gatcaatcaa
    27361 cagtggaata tcttccatgc gttccctcag cgggggaagg gtgattttga agacattgat
    27421 gcggtaaaac aaatcttccc ggaaacggcc ctgtctgacc agatcctcga gatttttatt
    27481 ggttgccgcg atcacacgga cattgtgctc aacgctttgt gttgcgccca gcggttcata
    27541 ggttttttcc tgcaggacgc gcaggagctt aacttgcatg gcgggggtga tttctccgat
    27601 ttcgtcgagc aataacgttc cgttttctgc cagtttaaaa cggccgggct tgtctttttt
    27661 cgcgtcggta aacgcccccg ctttatagcc gaacagttcc gactccagaa gtgtatccgg
    27721 cagggcgctg caatttaccg cgacaaagga cgttttgcgg cgcggactca ggttgtggat
    27781 ggcccgggcg atcagctctt tgccggttcc gctttcgcct tcgatcagca ccgtactgcc
    27841 gcctgccgca atatcgggca ggatatcgaa caactgcagc attttatgat tcttggaaag
    27901 aatatcggca aagttgtatt tctgtaaaac cgccttgcgt aaatcttctt cgacgctgat
    27961 gtcccggaat gtttccaccg ctcccagcaa ttttcccttt tgatcgcgca ggagcgccgt
    28021 ggaaatactg acgggaagtt tgtgcccttc cgcgttgatg atggctactt ttttattgat
    28081 gatctgctcg cccgttttca ttgtcttgcg caggcagcag tctttttcgc aaatatccgc
    28141 ttttaaaaca tccttgcagc gttggccgat ggcttcctcc cggggaaccc cggttatttt
    28201 ttcagcggca cggttgaaag acgtgatcag ccagtcctgg tctaccgtga aaacgccgtc
    28261 ggcgatcgaa tccagaataa tatcctgttt ggttccgata tccggcgatc cctgttttaa
    28321 aggcatcttt tttttcatgt ctgttttccg atgacgggtg tcggatttag tgacagccgt
    28381 gttcgtgctg ggtgcaggaa attcccgaat ctttcagtgt gccggcaagc cagctttccg
    28441 taactgtctt cacgtttccg gaacagccgc gcagaacatc aatgccatgg ctgttcaaca
    28501 cattgacggc gccctgtccc atattgccgg ccagcatcag cttgacgccc tttgcagcca
    28561 gcacttcggc gatgttggat ttacacccgc agccggcggg tgaagaaacc ttttcctcct
    28621 cgacaatttt cttttcctgg tcaatggtga aaaccgtaaa atactcacag tgtccaaagt
    28681 gttcatcaat ctgattctga taagagggta aagcaatttt catacatatc tccttgcaaa
    28741 gcatttttca tttattcctg cgttttgaat gttcgcgggt tttctattca tcaaaatcga
    28801 agtcgccgcc gattcttaat gcttttccgt tgatcagcac atcggcgatc tttttgtgtg
    28861 cgacgtctat gattctgccg aaggtttgac gtgaaatgtt catcttttcg gcggcttgtt
    28921 tctgatacaa atgttcgtag tcggcaagac gaaccgcctc gaattcgtcc atattcagaa
    28981 caatttcttc gagctccatg gagggtattc ccctgggctt gaagtatttc ttgtcgggcg
    29041 cacccatgat gtgacggtgg catttcggcc ggggcattgt ttgtatgaac tccttttgtt
    29101 agaatataag catatgctca aaacttgtca agggttaaaa tttcctcgta accgtattta
    29161 aagtcgttaa cgcgagtttt agaaaagata atcgttggag gaattcctac tgcccgatac
    29221 catttcgctt cttccggtga ccgtaagagg acatttgatt gttgtgaaaa aaaaacggct
    29281 gtggtaactt cttgcgcaat tcgatttttc aaagcgcaca agggtgtttt ttcatgcaga
    29341 agcatatcag caacgttcac gtgcacatgc cttattctct tttaccgaag tatttgaaga
    29401 tcattcttca gaacaaactg aatctggaaa tatactttca tcaccagatt ctgaatcgtc
    29461 tggataaatc tcgatgcatg gaaacggcaa agattcttga aagcgaggga ttgaaaataa
    29521 cgtttcatgc gccttttgtg gacttacggc cgggggccct ggatgagacg atccgccggg
    29581 ccagcattga ccgcatcagg caggtttttg atctggcacc ctattttcat cccctgaaaa
    29641 ttgtctgtca tgcgtctttt gacgaccgtt actatgttta tggcgacgat ttatggctag
    29701 agagcagcct caaaacctgg aaggagttca tcgggctggc ggacgatcac cggaccatca
    29761 ttgcgctgga aaatgtttac gaaaaaaatc ccgtgatatt gggcaagctt ttccgatctc
    29821 tggcgtccga cagaatctgt ttttgtttcg atacgggcca ttttaatgtg ttttcctgcg
    29881 aaccgttgaa tgtctggttc gatgaactgg gaaggtatac ggggcatttg catctgcatg
    29941 acaatttcgg atggcaggac gagcatcttt ctgtcgggca cggcacgttt ccctttgcgg
    30001 agttgatgca gtacatcaga aatatgaaga aaaaaccgac ggtcacgctg gaggcgcata
    30061 atcagaaaaa tctttgggca tcgctcgatc atgtcaaacg aatggatttc cccggtgttt
    30121 gaagataaga cggactgtgt tgggggagta ttgtgctgag gtatgtctta aaaagaacat
    30181 tgttcatgat tccgctgttg atcgggatta ccctgatctg ttttttcgtc atgcgtctgg
    30241 cgccgggttc tcccacggat ttgcagacgg agatgaatcc gaaggcatcc caggaaagca
    30301 aagagcggtt gaatgcgctg tatgaactgg ataagccgat ttatgtgcaa tacggatcat
    30361 ggctgaagaa gatcagccgc ggcgacctgg gcgtatcctt ctcgtccgat caccgtccgg
    30421 tggccgacaa gatcatggag cggctgccca ttaccatcct gatcaatttt ttatcgctgg
    30481 tgatcatcct tgctgtcgcg gtgccgatcg gcgtgttgtc cgccgtccac caggactcgc
    30541 ttttcgataa ggtgaccacc gtgctggtgt ttatcggttt tgcggtgccc acgttctggc
    30601 tggccctgct gctgatgatt tttttcggaa tccatctggg ctggctgccg atttccggtc
    30661 tgcgctcgct caattacgaa tatctgaatg tctggaatca gttgatcgat ctggccaaac
    30721 atctggtttt gccggttttc gtgtcagcgt tcggcggatt ggccggtctg tcgcgttata
    30781 tgcgggcgaa tatgctggag gtgattcggc aggattatat tatgaccgcg cgcgccaagg
    30841 gattgagtga aagggatgtc atttaccggc acgccctgca caacgcgcta ctgccggcca
    30901 tcactattat ggggctttcc attcccggtc tcattggcgg cagcgttatc tttgaaacaa
    30961 tttttgccat tccgggcatg ggacagcttt tttacatgtc ggtcatggca agagattatc
    31021 cgaccattat gggaattttg ctgatcggcg ctattctgac gctggtgggt aatctgattg
    31081 ccgatgtgtc gtacgcggtg gcggacccgc gtatcagaat atcctaaaag ggctgaaaag
    31141 atgacggcat ggacagccat ctataaaaac aaactgatgt tgaccggcgg cggactggtt
    31201 ctgacgcttt tgatcgtttc cattctggcg ccgtggctgt ctccctatga tccgggacaa
    31261 atcgatttga acaatgtgct ggcagctcct tctatggagc atcttttcgg aacggatcaa
    31321 ctggggcggg atgttctttc gcggatgatc tggggtgcca gaatttcttt aaaagtcggt
    31381 tttgtggcga cggggttggc cattctcgtc ggcacgtttc tgggagcatt gtcgggatat
    31441 tacggcggct ggatcgattc ggtcatcatg cggtttgtgg atatcatgct ctgtttcccc
    31501 acgttctttc tgattctggc ggtcattgct ttgctggagc cgtccatctg gaatattatg
    31561 atcattatcg gactgaccgg ctggatgggc gtaacaaggc tggtccgggc ggattttatt
    31621 tcgttgcggg aaagggattt tgtccaggcc gccagagcga taggcgccgg ggatgcgcgg
    31681 attatttttc ttcatatcct tcccaacgcg atggcgtcga ttctggttgc ggcgacgttg
    31741 gggattgcgg gggcgatcct caccgaatcg gccctgagtt ttctgggtat cggtgtgcag
    31801 ccgcccaccc ccagctgggg caatattctg acggccggca aggataatat cgacatcgcc
    31861 tggtggctgt cgctttatcc gggcctggcc attctgacga ccgttgtcgg ttacaatctt
    31921 ctgggggaag gcatcaggga tctgctcgat ccccgtttaa aaaaataaaa ccggggcctt
    31981 gtcaaccggt tcattcttga taaaaggacg aaaatataat aataacctct caacgaccta
    32041 tacgtaagga gaagaaacca tatgcccaac agagtcaatg cagaatccat cagtgacgat
    32101 aaacgcatcc tgaagcgcaa gctgatgcag ggcgaaatat ccgaaaagga tatacaggct
    32161 ctaataaaga agcttcccga tgtttccgaa aacgccgagg aagttcgcct ggatgatgac
    32221 aaaaaatagt attttatgct gatcgattcc catgctcatc tggaaatgaa ggagtttgat
    32281 gcggaccgtg atgacgtcat tagccgcgcc cgaagcgagg gcgtagattt tattgtcacg
    32341 gtcggaacca atctccggtt gagcagcaga gccgtcgcgc ttgccgaaca atacgaaaac
    32401 atctttgcca ccgtcggcat tcatccccac gatgtcgcca aagccggtga ggacaccctg
    32461 gatgctttaa agcaacttgt tctgactcat aaaaaaatcg tcgcctatgg tgaaatcgga
    32521 cttgattatt tccggaatat ttcacgtcag gaaatacaag tcgagatgtt cgggcagcag
    32581 ctggacttgg tgaaggaact gaatctgccg gttgtgattc atgatcgtga agcgcatcgt
    32641 caatcgctgg atatgattca ggcctccggt gtgcgtcgcg gcgtctttca ctgtttttcc
    32701 ggggattatg acatggccag aaaatgcatc gacctcggat tctacatctc catccccggt
    32761 gtggtcacct ttgacaaatc cgggaaaatt cgcgatgtcg tcgaacgggt tccgctttcc
    32821 tccctgttac tggaaacgga cgcgccgtat ctgacgccgg ttccgaaccg cggcaaaaga
    32881 aacgaacccg cctttatcgt ccatacggcg aaagcggtgg ccgccataaa aaaaatatca
    32941 tgggaagacg tggcggcgca aacgtctgtc aacacgatca atctcttcgg tctgaagatg
    33001 atccgtaacg tttagttttt tacggagccc cgttcatgaa gaaacaattt aaagccgtgg
    33061 ttttgttaag cggcggtctt gattccgcca caaccctggc gatcgcccgc gacaggggat
    33121 ttgaaattta cgcgttgagt ttccgctacg gccagcggca cgcggtggaa atgaaagctg
    33181 cccggcgtat tgcacgaaaa aacaaggtcg ccggacacct gatcctgaat atggatttac
    33241 gcaaaatcgg aggatcggcc ctgacggcgg atattcatgt gcccaagaat caaacaggct
    33301 cggtgctgaa aaggaaaatt ccggtgacct atgttccggc gcgcaacacg atatttcttg
    33361 cctacgcgct ggcctgggcg gaagtgatcg gcagtcatga tatctttatc ggggtcaatg
    33421 ctctggatta cagcggttat ccggattgtc gtccccggta cattgccgca tttcgaaagc
    33481 tggccaatct ggcaacgaag gcgggggttg aaggaaaaca aaaactggcc attcacgctc
    33541 cgctcattaa aatgagcaag gcggagatta ttaaaaaggg catggacctt ggcgtggatt
    33601 acagtctgac gcacagttgt tatgatccgt cgccatcggg agagccgtgc ggtgaatgcg
    33661 attcctgtct gctgcgattg aaagggtttc gtgaagccgg attgaaagat ccgctgaaat
    33721 attgccggta aggggctggt cttgggttat cgaatcaaag agatatttta ttccattcag
    33781 ggcgagggat tttatacggg acggcctgcg gttctctgcc gtttttcggg gtgtaatcta
    33841 tggtcgggga aagcatccga ccgcgaaaaa gcggtttgcc ggttctgcga tacggacttt
    33901 gtcggttgcg acggcccgga gggcgggatt tttcaaaccc ctgaagcatt aaccgagaaa
    33961 attattacat tgtggcctga aaaaccgtca gacacgctta ggccgtttgt cgtgctcacg
    34021 ggaggagaac cgctgctgca aacggataga gccttactgg acgctctgca tcatcagcat
    34081 gttgaagtcg ccgttgaaac caacggcacc attatgccgc cggaaggaat tgactggatt
    34141 tgtgtgagcc ccaaagcagg cacaacgatg gtgttacgtt cgggacagga attaaagctt
    34201 gtttatcctc agcagggagc cgagccggaa aagtatgcgc tgctcgattt tcagcattat
    34261 tttctgcagc cgcttgattc accaaaccgg caaaccaaca ccctcagggc cctggaatat
    34321 gttttggcgc atccgcaatg gcggttgagt ttgcagatac aaaaaatttt aaatgtaaaa
    34381 taaaggaacg caaagtggaa atcttcaagg tgtataaatt tgatgcggcg caccgcctgc
    34441 cgaaggttgg tgcagaacac aaatgttttg aactgcacgg acattccttt cgcgtggaga
    34501 ttcatgtgca aggtccggtc gatccgcagc tggattgggt gatggatttt gccgatatcg
    34561 accgtgcttt ccggcctctg ctggatcagc tggatcatca gaatttgaat gacatccccg
    34621 gactgaataa tcccatctcg gaaaacattg cgcggtggat ctgggaaaat cttcaggacg
    34681 cattgccgca attaaacaaa atcgttgtgc aggagagccc ggaatccggc tgcatttatt
    34741 gcggcgaaaa ataacggttt ggaggcgggg atgctccgca gaaaaaattt ccgtttagcc
    34801 gccgaaacgt cacggatgtc ttccgtttca gagcggttta acagtccctt ttttgttgaa
    34861 aaataatgcg gtcggtggta ggtgctgaaa caaacaaaaa gcagtgacca tcatggatca
    34921 gaagaccata cagaagatca aaggcaccca gcaggacggt ttgctggaag aattattgcg
    34981 ggaaaaggcc gcggtgctga cccgggcgag ctgggcggtt gaggatgtgc tggagaagat
    35041 gaagcgaatc gaaggagaaa tcgaaattaa aacatcgttg atccagtcga tgacgcacga
    35101 ggctgatcgc gcggttctgt cccgtcagaa aaaactgttc tgcgaagaaa taaactgtaa
    35161 gatagacgaa ttcaatgcca ttcgccggaa agcccagctt caatattatt atttgattgt
    35221 cacaagagaa gccctgggct tgaggcgtca tgagatggtt cagaaaattt accgtattcc
    35281 cgacaagaaa aaaaagatac aggtgttttt ttaatggaca ggttccgtaa accacgcatg
    35341 cggatggtag aaacgcagat caaggcacgt ggcatcagcg acgtgcgcgt gttgaaggcc
    35401 atgcaaacga ttccgcgcca tctttttgtc gatgaaggcc tgatcgaaca ggcgtacacc
    35461 gacagccctc tgcccatcgg cgaacatcag accatctccc agccgtatat cgtcgcgctc
    35521 atgacgcagg cgctggatct gaagggaagg gaacgggtgc tggaaatagg aacgggatcc
    35581 gggtatcaga ccgcggtgct ggcctgtctg gcggaccgcg ttttttccgt agaacgcatt
    35641 gccttgctgg ccactcaggc gcgcagaaca ctcgacgcgc tgaatatcta taatgtggcc
    35701 atcagagtgg gtgacggttc ttacggctgg aaggaagaag cgccgtttga cgccgttatt
    35761 acaaccgccg cggtgccggc gattccccgg tatctgctgg atcagttggt tccgggggga
    35821 agaatgattg tcccgtcggg tggccgcgat gtgcaaaccc tctataaact aacccgatcc
    35881 aaggaaaatc ctgaagagat tatacaagaa gaccttggag gctgccgctt cgtcagtttg
    35941 ataggggaga gcggatggag aaaatagcaa cgcgggcaaa accggttttt tttcttctgc
    36001 tggtctgtct gattttttcc tgctggcatc cccggctgca aaaggcccag gcggcgggcg
    36061 tttatcattt ggtgaaaaag caggaaacgg tctggagtat agcccggact tacggtgttt
    36121 ccgtgaagga tatggttacc atcaatcata tcgacgacgt gaatcagata gaggagggct
    36181 cggtgctatt cattccttcg gccgagcggg tcattgatga taccacccag ataaaaaaag
    36241 aaaaggtcga tgaagccccc aaaacggaca ttcgggattc gggcagaacc ggagatggga
    36301 aaaacagcaa aaaaaccggc ggcaaaaaaa tagccgctga ggcagccccc gtaaaaaccg
    36361 gcgatcttca agattccgca acggcaaaaa tccgggacga caaaccaacg gatccaaaaa
    36421 atcaaacggt tgaagaaatg gttgtcccgg accggaaaat aaaagcgaca cgcaacaaat
    36481 ttatctggcc ggtgcagggc gaaatcaagg cgcatttcgg tattcaaccc aataaaacgc
    36541 tccataactg ggttaaaatc gtttctgcgt cgggaaagaa aatcaaagcg gcggaatccg
    36601 gcacggtcat tttttcatcc catctgaaga attacggtga aaccattatt atccgtcaca
    36661 aagatcattt cgccacggtc tacacccatc tgaaaaaaag gctggtcaaa atggatacgc
    36721 ctgtcaaaaa aggcgaggcg atagccgtcc tgggggaaaa agacgatgac ggggcggttt
    36781 atatgaattt tgaagttcgt ctgaaaggga aagcggtcaa tccgctttca tatctgccgt
    36841 aattttttgc tgttgccatg acgcaaatca tccttatatt aaataaaaaa attattcagg
    36901 ttaacgatga aatgctattt tgaacagggc cccattcgtc ctcccagtga agcggcaagc
    36961 cttctgatcc gcgtcagcag aaattgtccg tggaacaaat gcgctttctg tcatacctat
    37021 cgggggacga aattcgagct gcgcagcgtt gaagacgtca aaaaagacat tcagaacatg
    37081 aaagagatag ccgatgaaat tgtccgtttt tcctggaaac tgggggaggg cggccgggtc
    37141 aaccgcaatg ttttgcgcgc catctacaac agtgaagatt tttatcatga caatatttat
    37201 tctgtggcgg cctggcttta tttcggcggt gaaaacgtgt ttctgcagga tgccaattcg
    37261 ctgattttaa aaaccccgga tcttgtttcc attcttgatt ttttaaagga aaaatttccc
    37321 gatgtgcaac ggataacatc ttactgccgt tcccacacgg cggcaaggaa gagcattgcg
    37381 gaattcgaac aattgaaaaa agccggactg tcccgcattc atatcggcat ggaaagcggc
    37441 tccgacgagg tattgtcctt cattcgcaag ggagtgacgt ctgccgacca tatcagggcg
    37501 ggactgaaca tcagaggcgc cggcatagaa ttaagcgaat atgtcatacc cggactgggc
    37561 ggagaacggt ttacccgtca gcatgctctg gaaaccgccc gggtcataaa tgccatcaat
    37621 cctgatttta ttcgtctgcg caccctgcat gtggtgccgg gcaccgatct ggatgagttg
    37681 atgaacaaag gtgaattcaa accgctggcg gatgacgata ttctccggga aataaaattg
    37741 tttattgaga atcttgacgt gcgcgggacg tatctggcca gcgatcatat tttgaatctt
    37801 ctggaggagc ttgaggggaa tttgccggat gaccggcaaa acatgctggc tgcgattgaa
    37861 aaatatttta aactttccgc cgaagacaga cttgttttcc gactgggacg gcgcagcgga
    37921 tcattgagga aactcgatga tctggccgat caggcaacat acgggagact gaggtcggtc
    37981 gtcgatcact atgctgctcg aggcgaaaat cttgatgagg atatcatcaa aatgatgaat
    38041 aatttcatat aaatatcggg gaaatatctt gtcgtccgta cagccttttc ctttatgtca
    38101 tcctgattcg ttttattttg ccatgtggta aatattttat tgacacgaag gttctttctg
    38161 cttatacttc cgccgtcaaa aggcaatatt ttatcgcgta tcaggcaatc ggcgtctcgc
    38221 accgcgtctg ctgatgatct gagaccattt tattcctggt ttcgaaagag cttgactatt
    38281 tgcagctcca taatggtcgg cagaaaagtt attctgcaga acattattca atgctacagg
    38341 aatgtgatcc gcaggattgc ccggtaggtt gataaaagga tgcaacgttt tgataaaaca
    38401 tcaaacaata atttaaggag gctatacaat gcgtgaatgt gtaattattg atggcgtgag
    38461 atcgccaaat tcccgggcac acaatgaaaa aggttggttc agagccctca gacccgatga
    38521 agttctgacc aaagtttatg atggtctgtt tgcccgtaac cccaaagtga aacccgaaga
    38581 tatcgaatcc gtatttgtcg gttgcgccaa cattaccggc atgcagaatg atatcggccg
//
LOCUS       DAOSME010000018        38293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1435_length_38293_cov_23.480491, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000018 DAOSME010000000
VERSION     DAOSME010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 38293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38293
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_38293_cov_23.480491"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLG98_04330"
     CDS             <1..255
                     /locus_tag="PLG98_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32511.1"
                     /translation="KAEAFFDYHAILKFQEQNEQLLNDFLSQATWMPDDFKTAAEKMA
                     DVYKDGCDKFRRYVEKNINGVSDFSSSSGKPGRKAGSRKK"
     gene            complement(307..1743)
                     /locus_tag="PLG98_04335"
     CDS             complement(307..1743)
                     /locus_tag="PLG98_04335"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOE32512.1"
                     /translation="MMKVVHRALILFLTVVFPAVVFAGGFGLDSSTADIGTLLPLWTT
                     LPFTGILLSIALIPLLLPRFWHRHFPKVSAFWAIVFAVPFIFFFREKALHEIAHIILI
                     DYVPFILLLWALFTVSGGIYVRGTLKGTPAVNTTVLLIGTLLASVIGTTGASMLLIRP
                     ILRSNAWRIHKVHTIVFFIFLVSNIGGSLTPLGDPPLFLGFLHGVPFFWTMHIFPEMA
                     VASVTLLLIYFALDSHYYKRENKTAIPEEEFEPLKIEGGRNFIFLAGIIGAVLFSGSV
                     RLGDVNILGIHQTTENLIKDAVLILMGLASLGFTAKEVRKNNDFGWAPMLEVAYLFAG
                     IFITIIPALAILKAGEKGALHWLIKAIDSPAQYFWAVGILSSFLDNAPTYLTFFNSML
                     GKFYPGMPEPAAVALLIVEKIPYLAAISVGAVFMGANTYIGNAPNFMVKSIAEEAGVK
                     MPSFFGYMFKYSIPVLVVLFIVMTFVFF"
     gene            complement(1740..2801)
                     /locus_tag="PLG98_04340"
     CDS             complement(1740..2801)
                     /locus_tag="PLG98_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOE32513.1"
                     /translation="MNLMQESYLGNTLQAWLIALSILIVVFIILKLIQAVAVRKLSRL
                     AVSTSNQIDDLLVGLLKRTKGFILIIAAAYLASLTITLKPSVGQLWQKVVILALILQA
                     GFWASSAIAFAIGNIIKKRSEEDFSSKTTITFLGFVARFVLWIVILLLILDNLGVNIT
                     GLVAGLGIGGVAVALAVQNILGDLLASLSIVLDKPFVIGDFITVDSLSGTIEHIGLKT
                     TRVRSLSGEQLIFSNNDLLKSRIRNYRRMSERRIIFNFGVVYQTPLEKLKTIKMIVRD
                     IIDKTETARFDRVHFKEYGDSSLNFEAAYFVQSSDYSTYMDVQESINLEIFRRFRDEG
                     IQFAYPTRTVFIQDEKQQK"
     gene            complement(2877..3287)
                     /locus_tag="PLG98_04345"
     CDS             complement(2877..3287)
                     /locus_tag="PLG98_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE32514.1"
                     /translation="MLNFKNILYPIDLNSEKISSLETALEFARLLNGALHIFYVNDIL
                     AGYRHPTDHEDAVALRVKETVDASLLENINIIYAVSKGETTEEIVKYAQDNQIDLIII
                     GHKHRGRLYASLFDSTDIDTIDKVFLPVLVIPEK"
     gene            complement(3608..4375)
                     /locus_tag="PLG98_04350"
     CDS             complement(3608..4375)
                     /locus_tag="PLG98_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE32515.1"
                     /translation="MGEAVTKEIRDGVAILAIDKPKMNQLSGEVFEAMRKHLDAVEAD
                     KAIRCVIITGAGEKAFSAGADLASGFGDFSAVDFLKRGQDIWNKVEYFPKPVIAAING
                     HALGGGCELAMACHIRFMKKGARIGLTETNLGIMPGYGGSLRLPRLVGKSKALEMIYL
                     GKQCEADEALSIGLVDHITEGNVVDAALELANKIVKRAPLAVAAVTKLCNISPSISAE
                     IHLKIEREELGKLFNSKDAQEGIGAFFEKREANFKGE"
     gene            4942..5709
                     /locus_tag="PLG98_04355"
     CDS             4942..5709
                     /locus_tag="PLG98_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE32516.1"
                     /translation="MAYDNIIVETKDFVTVVTLNRPPMNSINLGIREELDQIITDVQT
                     SKETRVVVVTGSGEKAFCAGMDVADVANIDKGPNGIDVCNKVERSLKPVIAVINGHAL
                     GGGCELAMACHFRFMTDNPKSQIGCPELNLALTPGWGGIQRMPRIIGKSKALDFILFS
                     KKVSGPDALAIGLVDKVFPAADLMKEAMHFAQALSKRAPIAVNAVLNGMNIGLEKGIV
                     AGLEADKQWIEKLKTSKDATEGVVAFIEKREPNFVGE"
     gene            complement(5954..6862)
                     /locus_tag="PLG98_04360"
     CDS             complement(5954..6862)
                     /locus_tag="PLG98_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE32517.1"
                     /translation="MSNISFEGKVVIVTGAGSGLGREYALEFARRGASVVVNDLGVAR
                     DGSGSTKNAADAVVTEIKNSGGKAVANYDSVATPEGGINIVKTAVDTFGTVDVLVNNA
                     GFLRDKSMMKMTADEWDAIMSVHLRGAFCVTQPALAVMREHNYGRIMVTSSTSGIFGN
                     FGQINYGSAKMGLVGFMNVLKLEASKFNVHVNAIAPTAYSRMTENLIPKELAPKLQAK
                     FNVPMVVYLCSEECQENGSIFTMGAGWYAKTAIVCATGACLGDGSRDIPAEEVRDNFA
                     KISDLTGAKPLYDSMGIFELMGPLLQ"
     gene            complement(6904..7656)
                     /locus_tag="PLG98_04365"
     CDS             complement(6904..7656)
                     /locus_tag="PLG98_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOE32518.1"
                     /translation="MFAWLTSPEAWIALATLTALEIVLGIDNIIFISILVSRLPKNKQ
                     NFARNTGLMLAMLTRLALLFSIFWVIKLTAPWFTVFGQEISGRDMILIGGGLFLLAKA
                     THEIHTSLEGAQEETPRLISSSVAAVLIQIALLDIVFSLDSVITAVGLAKDVSIMAIA
                     IICAVGVMLFAAKPISKFVDAHPTIKILALSFLLLIGVTLVAEGFDVHVPKGYIYFAM
                     AFSVGVEMLNLRMRNKQMKPVKLHKTVYNPEA"
     gene            complement(7690..8304)
                     /gene="cobO"
                     /locus_tag="PLG98_04370"
     CDS             complement(7690..8304)
                     /gene="cobO"
                     /locus_tag="PLG98_04370"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOE32519.1"
                     /translation="MTMVKTEDPAVYNVRSKKSSGLKTSKENAVPKQKGLVIVITGNG
                     KGKTTSAFGQALRAIGQGYKVFVLQFMKGRKYGEFIAAEKYLSNITIKMSGLDSFVMR
                     DHPAAIDIDMARKGLDIARKAIMSGKYDMVILDEINVALDFKLVDLKDVMKLIKNKPA
                     HVDLILTGRYAPKEIIKLADTVSDVQEIKHHYNAGIKDRAGIEY"
     gene            complement(8301..9275)
                     /gene="meaB"
                     /locus_tag="PLG98_04375"
     CDS             complement(8301..9275)
                     /gene="meaB"
                     /locus_tag="PLG98_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOE32520.1"
                     /translation="MDATIKKICAGDVRAASRLIRDIEDKRPDAKVKIKKLYAHTGKS
                     FIIGITGAPGAGKSTLTDALISEFRKLNKTVGVLAVDPTSPFTGGAILGDRIRMQRHA
                     ADDGVFVRSLATRGALGGLSQAAGNAIHVIEAMGKDIIIVETVGIGQQEIDIINHAHS
                     VVVVLVPGMGDEIQTMKAGLMEIADVFVINKANRPGTGQLQTELSSMLNLSPKTADGW
                     KPPIVLVGDAFERATFDENTAELARKIEEHHCFLEQSGQLTERMHRKVMAEIKDALGA
                     CLMEPLMQSLISSGEMGKLSEKIKNRKSDPYTVAEDITEKFFQKQVKK"
     gene            complement(9294..9695)
                     /locus_tag="PLG98_04380"
     CDS             complement(9294..9695)
                     /locus_tag="PLG98_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOE32521.1"
                     /translation="MSERKIRVMVAKPGLDGHDRGARILARCFRDAGFEVVYTGCHQT
                     PEQIAASAIQEDVDLVGLSCLSGAHRALFPRVVELLREKGAGDIAVIGGGIIPEQDVP
                     MLYESGIKAIFTPGTALDSIVEWIKKNIKTH"
     gene            complement(9843..11525)
                     /locus_tag="PLG98_04385"
     CDS             complement(9843..11525)
                     /locus_tag="PLG98_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOE32522.1"
                     /translation="MYFSEEMQEASLKLTQKWEKEVQKTLKNNPEQKKRFSTVSDTEI
                     KRLYTPEDIKDINYTEDIGVPGEFPYLRGNQVTGYRGRYWTFRMFSGMGSAQDTNRRW
                     HMLLREGQTGLSTAFDFPTLMGYDSDSPKALGEVGKCGVAIDTLEDFLTLMEGIPMDK
                     VTTSMTINPPATVLWAMYCAAADIKGIPLSKIGGTIQNDMLKEFIAQKTFMCPPEPSV
                     KLISDTVEFGTKYVPRWNTISISGYHIREAGATAVQELAFTIRDGIEYVDDVIRRKKM
                     NVDDFASRLSFFFNSHIDFFEEICKMRAARRMWAKIMRDRFHAKDPRSLWLRFHTQTA
                     GCSLTAQQPYNNVIRTTVEALAAVLGGTQSLHTNSLDEVLCLPSEHAVEIALRTQQIL
                     AEETGVANTIDPLAGSYFIESLTNEMEEKAWDYIHKIDAMGGMITAIEKGFPQMEISD
                     AAYRFQKQLDAGEKSMVGVNKYATDVTHPLPLVDIDEKVGEEQIKRLKSVRRKRDSKA
                     LKKSLEDVRNACKKGNNVMPCCIDAVKNLATLQEICDVYRDVYGEYRDPGLY"
     gene            complement(11735..11935)
                     /locus_tag="PLG98_04390"
     CDS             complement(11735..11935)
                     /locus_tag="PLG98_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOE32523.1"
                     /translation="MPEGKVKWFNANKGFGFIQQDEGGDVFVHFSAIQVEGFKTLNEG
                     QRVSFDIQQGKKGLEAENVKPI"
     gene            12336..12908
                     /locus_tag="PLG98_04395"
     CDS             12336..12908
                     /locus_tag="PLG98_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOE32524.1"
                     /translation="MKIKASVLIVLLAALWMTVSAGAAEVKPPQVGQGFPDVELSKPV
                     IAGELKYLGLSGSGSFRINQIKADVVIVEIFSMYCPHCQAEAPAVNELYQLIEENAAL
                     KGKIKIIGIGTGNSIYETDIFKKKYSVKFPLVPDGDFKIHKIMGEVRTPYFIVVKLKG
                     KKADVIYSKAGALGDNKTFLKEIIKSAGLK"
     gene            12918..13505
                     /locus_tag="PLG98_04400"
     CDS             12918..13505
                     /locus_tag="PLG98_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOE32525.1"
                     /translation="MKKKIILAALLICFLAPGAFALSDVFKNNVYETGELKPQDSVLK
                     VKVGQPAPDFTLKAVSGKTISLKDYRGKKNVVISFVPAAWTPVCSDQWPGYNIVKDIF
                     DVNDAVLLGISVDNIPTLYSWTNQMGHLWFDVLSDFWPHGAVADQFGVLRSDGISERA
                     ILIIDKKSIIRYIDVHNINKRPPLESIVRELEKLN"
     gene            13673..14308
                     /locus_tag="PLG98_04405"
     CDS             13673..14308
                     /locus_tag="PLG98_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE32526.1"
                     /translation="MEEKKRNRKRKEKENRRSTILKAARKLFLERGFKAVTVDSIAEK
                     AEVSKGSIYLCFESKEEIYTQILIADNISLNERIQNFSLIEASASQLLLEFARIYVDF
                     FLSDKELFRILMTFMMQTGQMHLTQQQSDELIRSTNENIKVISEIIQKGVDSGEFRPL
                     DNILQMQNAIWGMLNGVISLFLFTGNPIKRMERIHSTVQESMNVFIRGLKA"
     gene            complement(14383..14724)
                     /locus_tag="PLG98_04410"
     CDS             complement(14383..14724)
                     /locus_tag="PLG98_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE32527.1"
                     /translation="MEIKYKIWIEENGKVIFGKGRDEILKSIEQQHSLNAAAKELGMS
                     YRAAWGRLKASEERMEKKLVETSEKEKALQLTAQARTIIERFEKLEKDVENILKEAEQ
                     EFKKLFQTMGK"
     gene            14948..15364
                     /gene="nikR"
                     /locus_tag="PLG98_04415"
     CDS             14948..15364
                     /gene="nikR"
                     /locus_tag="PLG98_04415"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOE32528.1"
                     /translation="MPGLVRFGVSLEEDLLVKFDRHIKQKKYTNRSEAIRDMIREELV
                     RKEWTENKEVTGAITLVYDHHTRELVTKVLDVQHDYHTCILSTQHIHLDHHNCFEIVV
                     TRGKSKEIEELFQKLKSIKSVKHAGFMMATKGKDLP"
     gene            complement(15379..16113)
                     /locus_tag="PLG98_04420"
     CDS             complement(15379..16113)
                     /locus_tag="PLG98_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32529.1"
                     /translation="MSHHIVEFKNVSFRYPDGTQALQDISFRITHGESVGIIGANGAG
                     KSTLLMHLNGYLMPTSGTVNIGDVYLTKKTQPEIRRKVGIVFQNPDDQLFMPTVYEDV
                     AFGPLNLGLDETAVRERVEFALHLVNSLDLKNKPPHHLSCGQKSAVAIASVMAMEPDI
                     LAMDEPAANLDPRSRRLLINLLKTFEHSKIIASHDLDLILDVCHRCIVIKEGRVAADG
                     PVSEILTHQELLEENNLELPLSMQKR"
     gene            complement(16110..16919)
                     /gene="cbiQ"
                     /locus_tag="PLG98_04425"
     CDS             complement(16110..16919)
                     /gene="cbiQ"
                     /locus_tag="PLG98_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOE32530.1"
                     /translation="MSFDIQYYNIGFLDQLSYKSTFVHRLDPRVKLCVTLLFLLTVIS
                     FSKYEIFALLPFFLFPVLLMTLGEIPFMFIFKKVLLVSPFAVFIGIFNPLLDSGQISI
                     VNGYTISAGWLSFISILLKFTLTVSTALLLVATTSFPGVCHALRQLGLPSVFVSQLLF
                     LYRYIFVLMEETMRIVRARNMRSFGDRGKGIKIFVRIAGTLFLRTVQRAERVYNAMLS
                     RGFQGDMPALKHFQFKTGDMIFAATVAAFLLIFRFCPATEILGRFAQGVFS"
     gene            complement(17022..18056)
                     /locus_tag="PLG98_04430"
     CDS             complement(17022..18056)
                     /locus_tag="PLG98_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOE32531.1"
                     /translation="MHMADALLSPAVGATMWAGSVAAIGYSSKKLKENIDNKTIPLMG
                     VMGAFVFAAQMINFTIPVTGSSGHIGGGMLLAVLLGPYAAFLAMASILTVQALFFADG
                     GLLALGCNIWNMGIYPCFLVYPLIYKPVAGDGKSARRVSVAAILGAIVALQLGAFSVV
                     LETLFSGISELPFGTFVLLMQPIHLAIGLVEGIITAGIILYVQQARPEILHAHAAARP
                     LDAGLSIKNILAAFVILAVITGGALSWFASTHPDGLEWSIEKVTGKGELEEATQGIAP
                     VLKDIQEKTAFLPDYGFKPPADEQNTEESPSVWPGIEPGTSVAGVVGSAIVLMFIIFI
                     GFGIRLFKKQ"
     gene            complement(18101..18883)
                     /locus_tag="PLG98_04435"
     CDS             complement(18101..18883)
                     /locus_tag="PLG98_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOE32532.1"
                     /translation="MIRKHFINGLVWAVLFFIAGPSWAAHPLITDDTGTQGKGNFQLE
                     LTGQYDQDKENVGGVSIKATGVETAATLSYGMIDNIDLVLSLPYLWGREKENGSLVYK
                     EDGIGDASLEAKIKVFEKEGFSLALKPGISFPTGDDDKGLGTGKFGGQIFFIASQEVE
                     PFAFHVNLGYIRNENDADERENIWHASVAAAWEIVKDLSLVANIGVERNPDGEADDDP
                     AFLIGGIIYSIAENLDIDLGVKYGLTDSEADISALAGLAFRF"
     gene            19096..22164
                     /gene="fdnG"
                     /locus_tag="PLG98_04440"
     CDS             19096..22164
                     /gene="fdnG"
                     /locus_tag="PLG98_04440"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:19681..19683,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOE32533.1"
                     /translation="MHISRRGFLTLSSAVGGGIALSSLGLNLKPTRAYADELDKMNRV
                     KAAKQTTTVCCYCSVGCGLVCSTDKATGKIINIEGDAEHPINEGSLCAKGAGFFDLTE
                     ANKHRLKKVLYRKPYGTEWEEKDWNWALPRIARLIKDTRDKGFVTKNAKGELVNRCET
                     IGHYGSSNVDNEECWLMSVKCRALGLVYIDHQARVUHSPSVAALGESFGRGSMTNHYC
                     DLKNADVILIMGSNAAEHHPIAFKWIMRAKEKGATVIHVDPRYTRTSARCDFHVPLRS
                     GTDIAFLGGMIKYILENNKYFKDYVVNYTNASFLVNKDFSFNDGLFSGYDSQKRKYNK
                     DTWTLDKDANGIPKRDMTLSDPRCVFQMLKKHYSRYTLDKVSSITGVSKDNLLKVYKA
                     YSATGAPDKAGTECYALGWTHHTVGVQNIRSMAIIQLLLGNMGIAGGGINALRGEPNV
                     QGSTDHCILYANLPGYLKMPAASLDTLEHYLKKCTPESKDPQSANYYSNYPKFFVSFL
                     KSLWDDKVTKENDFGYDWLPKIDDGKHYSTMHMFDAMYEGKIKGYFCVGADTAVSTPN
                     SNKVRKAMENLDWLVGENIFNNETYEFWRGPGVDPAKIKTECFLLPASASMEKEGSQS
                     NSGRWVQWKYKAAEAPGDAIPVGEIEIKIMAEVKKLYQKEGGPNASAITNLKWDYLDS
                     KGHMDVLKVSHQVNGLFTKDTVIEDKAKGTSTLFKKGQCVPTFGNLKDDGSTACGNWI
                     MAGSFDEKGFNKMTKRGKDDPTGLGLFPNWSFAWPVNRRIIYNRASCDIYGKPYNPKR
                     NILEWVGDKWVGDVPDGPWPPQADKAKGKYPFIMKQDGVASLFGPAMAEGPFPEHYEP
                     LESPLAKNPMSGQLNNPAIEIFKGEMDKVASASEKFPYVCTTYSCTEHWCTGALTRWQ
                     AWLLEMQPELYVEIGDKLAKEKGIKNGDRVKVSSIRGEAPCVAMVTKRFKPFTVEGKT
                     VHQVGMPFNYGWLFPKDSTSDSTNVLTPTVGDANVFCPEYKAFMVNVEKA"
     gene            22177..22980
                     /locus_tag="PLG98_04445"
     CDS             22177..22980
                     /locus_tag="PLG98_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE32534.1"
                     /translation="MGNELVKLIDTTLCTACRGCQVACKQWNEMPGFRTKQTGTYQNP
                     PDLQWNTWTLIRFQEYEDKDENFQWIFRKDGCMHCTDASCVKVCPSGALYYTDIKTVG
                     IHRDKCIGCKECIAACPFDIPRYDKKTEKIYKCDLCYSRIKENLPPACVKSCPTGALT
                     IGPKDEMIKKAYARVQQLGGDANVYGDKFVGGTHVIYVLQFKPEVYDELPQDPKVPVS
                     TFIWKDVLKPLSLLAAGGVIGGSLLHYFTHGPHKPHPEHYDQDTKQGGA"
     gene            22982..23629
                     /locus_tag="PLG98_04450"
     CDS             22982..23629
                     /locus_tag="PLG98_04450"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HOE32535.1"
                     /translation="MKSKMVMVTSKYERFVHWLLAISCLLLCYTGLGIMYKELNFLGF
                     IFGGLKGLATVHDIMAVVFAISLVLAIAMWWKEAGIFVFPEDWEWIKAAGGYLWHLDH
                     VPETGKYNPGQKAFFLTVALFGLLMICTGFFMWYPHVLPAALMDWVYVLHVLGFVVIF
                     AFFFVHLYLGTIGNPGSVSAMISGKMELPVLRMLHPKWVKEMEHEGKLMIADDKK"
     gene            23829..24707
                     /locus_tag="PLG98_04455"
     CDS             23829..24707
                     /locus_tag="PLG98_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOE32536.1"
                     /translation="MTQEQTSEIKNIIDRALEQNPENKEIIKAFAPIIIRQRQLAEKP
                     SEQKLDCLAIDKQKLKAGVPVSRQINLILPGDPLKEIALSLAEAVKEGMPQLTENIDL
                     IAGLIKDDKIKPADYFKSTPDGESMATDVWAKDLKVSPSNASFLMSLVSRVILEQRAN
                     EITAAIGAFDWEKGYCPICGEFPSVALIEEEGGKRFLHCSACGYYWRFTRISCPYCEK
                     ETRQGMEYFYVENKKQESAFVCDQCKKYLVTLYRAGQVFARDMDVSAISLIHMDLLMQ
                     NKGYEPMTACTWNVLS"
     gene            complement(24895..26061)
                     /locus_tag="PLG98_04460"
     CDS             complement(24895..26061)
                     /locus_tag="PLG98_04460"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOE32537.1"
                     /translation="MAEISVNGFCDPAFDKVKQAFIGNFKKGKERGAAVSIVIEGKTV
                     VDIWAGYADFWKRRPWQKDTLVNIYSTTKGVTAICAHRLIEQGRLDPDKPVSAYWPGF
                     EKNGKEAITVRMLLNHTAGMVAIKKRQPGKALYDWDAMVNALENQSPWWKPGKLGYHA
                     ITFGWLVGQVIRNITGMSVGQFLKKEITGPLDLDIHIGLDEAEHHRSANMVMVRIPTI
                     HPDCLKLTRAIVLNPFGATSMALGNPFSIATGVNSRSWRSAEIPSANGQAAARALAKL
                     YGILANGGKAGNIQVLSPGEIDRCSEETSGGDDAVLKLKTRFSLGFMLNQDNPSGNMG
                     PGKKTFGHPGAGGSLAFADPDAHLGFGYVMNKMDTFILVDPRAKRLIKAVYACL"
     gene            26602..29298
                     /gene="fdhF"
                     /locus_tag="PLG98_04465"
     CDS             26602..29298
                     /gene="fdhF"
                     /locus_tag="PLG98_04465"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:27670..27672,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOE32538.1"
                     /translation="MEKKIPIRIDGKDVEATAGQTILECARDNGIFIPTLCHYTKTTN
                     VGACRVCVVEVEGARSLAASCCMPVTPNMVVRTDTEAVRKAQKMIIELLWSSGDHNCL
                     TCEQNGNCELQNLVYWLQIDKPRFEIQVPGYAKDDSNTMIARDMNKCILCGRCVRACN
                     EIQVNEVLDFANRSYKAKVGPAFDQDYIDSTCVFCGECVQACPTGALTFKQAKFAGRP
                     WELAKVKTTCTYCGVGCQMDLYTKDNKIVKVMGNREYGQPNDGSLCVKGRFGMDFLSH
                     PDRLKTPLIRYKKNENFKEATWDEAFTFIADKLSRIKKEHGADSIAGLASARCTNEEN
                     YLFQKFLRAGIGTNNVDHCARLUHSVTVAGLAATFGSGAMTNSIDEIEFTDLILATGT
                     NTTENHPVIGAKVKRATKRGAKLIVIDPREIDLVKYADIWLRQNPGTDVAVINGMMNV
                     IISEGLYAKEYVKDRTEGFDALKKMVEKYTPEKVEKISGVPAEDLKKAARMYAKADRA
                     SILYAMGITQHITGTDNVKSCANLSMLCGNVGIEGGGVNPLRGQNNVQGACDMGALPN
                     VYPAYQQVANEDVRKKFEGFWNAKLSPKPGLTIMEIMAAAGKGDIKAIYIMGENPMLS
                     DPDLHHVKKELQKLDLMIVQDLFLTETAELADVVLPVASFAEKDGTFSNTERRVQRVR
                     KALDAPGEAKADWEVICGIANKMGYEMNYASAREIFEEVAKVTPSYAGISYERLEKGG
                     IQWPCPTPEHQGTKFLHKDKFSRGLGLFTAIEYIPPDELPDKQYPFLLSTGRVLYHYH
                     TGTMTTRTKGLTERYPESLVEINPADAEKMGIAEGQKVKVTSRRGTVEAKATITKKSA
                     PGSIFMNFHFADASVNLLTNPALDPIGKIPEYKVCAVKVEAA"
     gene            complement(29471..30988)
                     /locus_tag="PLG98_04470"
     CDS             complement(29471..30988)
                     /locus_tag="PLG98_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32539.1"
                     /translation="MRKGLRLFPMMTMLILFVLTVFSVEIFAWPVIPPPNFTNEPPRN
                     PFIADSPWPMSHRNPYGQASSPYPGPKSINLTTRSSFHVGMPGLITLAISGKYPDGKR
                     VIWGSSSTDVFKADESLNYIKTMKKENLTISSIFTPGEMLSGAYTLVDKDNTFYMPRF
                     TKIFAFGDAIAGNRYSDIIIQGVYEIPANLLVDPAEKIVGLSMTWDGMLTLATSHGLI
                     GVVSRFFDEAYYYRFPADEQISNSIACDEHGGIYVVTDKNMYRVQWTGSELTIDPDKG
                     GWSAGYETGEASGVRLGAGSGSTPTLMGTGNQDKFVVITDGQNLMHLVLFWRDEIPAD
                     WQQIPGTKSRRIAAQVPVTFGNPGATETLSEQSVCVRGYGAVVVNNQLKASLDNQVIS
                     VLMSGFAPIAPYGVEKFVWDPWTRTLKTAWVNKSVSIPNGIPTMSQTTNLFYGVGKRF
                     GFWTFEALDWTTGKSVFSYPYGITPANNSAYAATEIGLNGSLLTGTLTGMVLMKP"
     gene            31211..32248
                     /locus_tag="PLG98_04475"
     CDS             31211..32248
                     /locus_tag="PLG98_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOE32540.1"
                     /translation="MRISLKSKPVIAPYRSTHNLRVIIQWAEEHGIDTASILSSEGLE
                     KADFDNPDRLITFEQQFEISQKLFDLISCPALGLRIGERINIGQLGKWIMAINSCATT
                     LDAFKMAFELADLLSFPFQFILDVQDDIAKFSFIDLIDLGRSSRFRYEMHMVGGYNIC
                     CEIMRERVQLIEIRFAYSKPEYAEEYKPIFNCPILFDAEETQIRFDSSYLTRPLPCAN
                     TLTKKIYEKDCRQTLEQLIMLTTTTELAKSALQFKDKELLTRSQLARCLNMSDQTLKR
                     RLAAEGVSYKSLVKDIRQKKACDLLQSTELTMEEIANKVGYRDVANFYRAFKSWTGLT
                     PRSCRGKNEKR"
     gene            32256..32396
                     /locus_tag="PLG98_04480"
     CDS             32256..32396
                     /locus_tag="PLG98_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32541.1"
                     /translation="MDELFIAEAIIRQVLRRMPDAPDRLLIAPKYSGSIEGRRLNQRK
                     NR"
     gene            complement(32489..33346)
                     /locus_tag="PLG98_04485"
     CDS             complement(32489..33346)
                     /locus_tag="PLG98_04485"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOE32542.1"
                     /translation="MNKSKDKLSALIIYLSPHGTTKKAIDTLAVSLNENSVSSDIYNL
                     SEFDNNDIMAKIADYQLIVLGSPTYFHHAPTVFTDFINNMPDASNDQSVVLLSSFGGV
                     SSGVVLYDLAKILHRKKYNLIGGIRVLTEHCLTFQEEKPFYSGHPDKKALNAIKTFGK
                     EISVRLKNKICTRYSPSDFNDKPFILNFMDDHIVKLRNFVWAMPGITVNQSLCNRCAV
                     CVKNCPTHNIKLGKATMHGGNCIYCYCCVRNCPVHATIAFLKPASITVKLMAEIFRKF
                     EDQETQQIV"
     gene            complement(33504..33818)
                     /locus_tag="PLG98_04490"
     CDS             complement(33504..33818)
                     /locus_tag="PLG98_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOE32543.1"
                     /translation="MSIKYDGKRKVKPTHPGEILKEDFMPDYNLNVATLAKALGVSRQ
                     TVNELLRESRALTPIMALRLGRLFGNTAEFWLNAQRAHDLWEAQQTHQAEIKKIHPLK
                     AA"
     gene            34811..35296
                     /locus_tag="PLG98_04495"
     CDS             34811..35296
                     /locus_tag="PLG98_04495"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOE32544.1"
                     /translation="MKLTETQMIEIAQHAFTRMDGAWFLALAKKFGVQTAWEMDVEAW
                     KHFAYVFGKNLRKNYLPNPVWPESIFDALDIFSRVLKIEGRKVCIEDGKIIIRITDCE
                     TQKAIAKAGVADCGIVTVQTYQGLIGGLFDNKIFVDVAHTKNLNRGDDCCEVVVTRKS
                     P"
     gene            35574..36023
                     /locus_tag="PLG98_04500"
     CDS             35574..36023
                     /locus_tag="PLG98_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOE32545.1"
                     /translation="MKIYFAGAIRAGRQDAEIYKAMIEVLMSFGDVLTEHVGNPALSE
                     KGNDGPHDRFIHDRDMAWLESCDCVVAEVTKPSLGVGYELGWATAMKRPVLCLYRRQS
                     KKVLSAMIAGSPGIRTVAYSSMDEARSIVEKFIKKAVQKISTSSGRE"
     gene            36024..37850
                     /locus_tag="PLG98_04505"
     CDS             36024..37850
                     /locus_tag="PLG98_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOE32546.1"
                     /translation="MVTSLELLAPAGDAAIGIAAIDHGADAVYIGAPKFSARAAAGVQ
                     LDDIARLIEHAHFYRAKVYVALNTILTDSELAESLDLIRAIDALGADGLIIQDVGLLE
                     LDLPPIPLIASTQMHNSTPEKVRFLEAVGFQRVILARELSLSEIAGIRRQAPKIELET
                     FVHGALCVSYSGQCYMSHAVAGRSGNRGVCAQPCRSHYTLIDGDGNTIMKNKYLLSLK
                     DLNLMSRVPDLIAAGSTCFKIEGRYKGMDYVKNVTAAYRQVIDRFINEHAGYRRSSSG
                     ASELAFTPDVGRTFNRGYSQHFVGGKREKIASLDTQKSIGQYLGKITAAGKDYFQIDR
                     DDLQNGDGLCFFTRQNNLAGSRVNRVEKGKVYPNSMKGLEAGLSLYRNLDTAFAKALK
                     KTSAKRLIAVEMDFSQADTLIRLAAKDEDGNQVEAVKDVLFEPSRNPAGARGQIEKQL
                     TSAGNTPFRISRLTIEPRAPGYLSASVLNGIRRDVLENLARVRRETYVRGDRPFCPND
                     VAYPEKHLDFHANVFNAAARRFYERHGATVTEPAFETLSDFAGKTLMTTRYCIRYQLD
                     LCPRLQQPESSVREPLYLRDAHHTYRLEFDCKHCRMSVILEN"
     gene            complement(37948..>38293)
                     /locus_tag="PLG98_04510"
     CDS             complement(37948..>38293)
                     /locus_tag="PLG98_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32547.1"
                     /translation="YFGKAGAKAVLSPTGPYRSFMQMTNDMKQFIETVLPKVWSSYFD
                     SGVAVAKFENNVAHIKITGLEIKYTYFEYLVMGYFQQAIKMFGKKSSAKRIRSLAAGD
                     KDMYFQYALKDS"
BASE COUNT         9907 a         9284 c         9412 g         9690 t
ORIGIN      
        1 aaggcggaag cttttttcga ctatcatgcc attctcaagt ttcaggaaca aaacgagcaa
       61 ctgcttaatg attttttgag tcaggcaacc tggatgccgg acgatttcaa aacggcggcg
      121 gaaaaaatgg cagacgtcta taaagacggc tgcgacaaat tcagacgata cgtcgaaaaa
      181 aacatcaatg gcgtcagtga cttttcatcc tcgtccggca aaccgggaag aaaggccgga
      241 tcccgaaaga aataaaaatt ccccggacaa tcaaattaat gcatcagccg ttttgtgtgt
      301 gcaattctag aaaaagacaa aggtcatgac gataaacagg accaccagga cggggatgga
      361 atatttgaac atatatccaa agaaactggg cattttgacg ccggcttctt ccgcaatgga
      421 tttgaccatg aaattcggcg catttccgat ataggtattg gcgcccataa aaaccgcgcc
      481 aacggaaatg gcggccagat aaggaatttt ctcaacaatc agcagcgcga ctgccgcagg
      541 ttcgggcatt cccgggtaga atttgcccag catactgttg aaaaaagtca ggtacgtggg
      601 ggcattatcc agaaaactgg aaaggattcc aaccgcccag aaatactggg caggggaatc
      661 gattgctttg atcagccagt gcagggcgcc tttttcgccg gcttttaaaa tggccagcgc
      721 cgggatgatc gttatgaaaa ttccggcaaa aagatacgcc acttccagca tcggagccca
      781 gccgaaatca ttgtttttcc ggacctcttt ggccgtaaag cccaacgatg ccagccccat
      841 cagaatcagc acggcatcct tgattagatt ttctgtggtc tgatgaatcc ccaggatatt
      901 cacgtctccc aggcggacac tgccgctgaa taacacagcg ccgataatgc ccgccagaaa
      961 aatgaaatta cgtccgccct ctatttttaa aggttcgaat tcctcttcgg ggatcgcggt
     1021 tttattttct cttttgtaat aatgggagtc gagtgcaaaa taaatcagca gcagcgtgac
     1081 gctggccaca gccatttccg ggaatatatg catcgtccag aaaaaaggga ccccgtgcag
     1141 aaatccgagg aacaggggcg gatctcccag gggggtgagc gaaccgccga tattgctgac
     1201 cagaaaaata aaaaaaacga tggtatgcac tttatgaatg cgccaggcat tggaccgcag
     1261 aatggggcgg atcaagagca tggatgctcc cgtggtgccg atgacggatg ccaggagggt
     1321 gccgataagc agcaccgttg tgttgacagc gggagtgcct tttaatgttc ctctgacgta
     1381 aattcctccg gatacggtaa ataaggccca cagcagcagg ataaagggaa cgtagtcgat
     1441 caaaatgatg tgggcgattt catgaagcgc tttttctctg aaaaaaaaga taaaaggtac
     1501 ggcaaaaaca attgcccaaa aggccgacac tttcggaaaa tgacggtgcc agaaccgggg
     1561 aaggagcaga gggatcaacg cgatggaaag cagtatgccg gtaaagggca atgttgtcca
     1621 gagaggcagt agggtcccga tgtctgcggt tgaagagtcc agcccaaaac cgcctgcaaa
     1681 aacgacggcg ggaaaaacca cggtcaagaa taatattaaa gcacgatgga caactttcat
     1741 catttttgtt gtttttcgtc ctggataaaa accgtcctgg tcgggtacgc gaattgaatg
     1801 ccttcgtccc ggaatctgcg gaatatttcc agattgatgg attcctgaac atccatataa
     1861 gtgctgtaat cggagctttg gacaaaatat gccgcttcaa agttaagcga tgaatcgccg
     1921 tattctttaa aatgaacccg gtcaaaacgg gcggtttctg ttttgtcaat aatatccctg
     1981 acgatcattt taatcgtctt caatttttcc aggggggtct gataaacaac gccgaaattg
     2041 aaaataatac gtctttcgga catgcgccgg taattgcgaa tgcggctctt taacagatcg
     2101 ttgttggaaa aaatcagttg ttctccgctg aggctgcgga ctctggtggt tttcagcccg
     2161 atgtgttcga tggttccgga aagcgaatcc acggtgatga aatcgccgat gacaaagggc
     2221 ttgtccagga cgatggagag agaggccagc agatcgccca gaatattttg caccgcaagc
     2281 gccacagcaa cacccccgat gcccagccct gcaaccaggc cggtaatatt gacaccgagg
     2341 ttgtcaagaa tcagaagcag gatgacgatc caaagaacaa aacgggcgac aaatcccaga
     2401 aaggtgatgg tggttttact cgaaaaatct tcttcggaac gttttttgat aatattgccg
     2461 atggcgaagg cgattgcact actggcccag aaacctgcct gtaatatcaa agccagaata
     2521 acgaccttct gccacaattg acccacggac ggtttgagcg tgatagtcag cgaagccaga
     2581 taagcggcag caatgatcag gataaatcct ttggtccttt tcagtaagcc gaccagaaga
     2641 tcgtcgatct gattgcttgt ggataccgcc agacgtgaaa gtttgcggac ggcaaccgcc
     2701 tgaataagtt ttaaaataat gaatacaaca attaatatgg ataaagcaat cagccaggcc
     2761 tgtaaggtat tgcccaggta ggattcctgc attaaattca ttttttctcc ttgtcagcta
     2821 ttgatgacgg ttttgaatac ggcaatctct aacgccgatt atcggatttt ctgaaattac
     2881 ttttccggaa taactaaaac aggcaggaaa actttatcaa tcgtgtcgat atccgtgctg
     2941 tcaaatagac tcgcataaag cctgccgcga tgcttatgac cgatgatgat caggtcaatt
     3001 tggttgtctt gcgcatattt gacaatttct tccgttgttt ctcccttgga gacggcatag
     3061 atgatgttta tattttccag aagcgatgca tcgacggttt ccttgacccg aagagccaca
     3121 gcgtcttcgt gatcggtcgg gtgtcgatac cctgccagaa tatcatttac atagaaaata
     3181 tgaagagcgc cattcagtaa ccgggcaaat tccagcgctg tttccaggga agaaattttt
     3241 tcggagttca gatcaatggg atataaaata tttttaaaat tgagcatatc tttctctctg
     3301 aataactgta caactaatat aatcgaaaat atatgtcaac cttttctggt ttgccgggac
     3361 agggaccatc tctgagaaga gaagacatta gaaaatgatg aatttggtaa tattgttggc
     3421 gagtgttcaa tcccgctccg ctgtgctcaa tgataatttc caatcccact ccgctgcgct
     3481 tcggggataa ttcgaccagc aaacttcagt gcactgcgtt tctgaagttt gggtctcatt
     3541 aaaaagcctc cgtcgcctga tagactggca acggaggctg gagattcgta tcagcagcag
     3601 ctgtttctta ttctcccttg aagtttgctt ctctcttttc aaagaaggcg ccgatgcctt
     3661 cctgcgcgtc cttggagttg aagagttttc cgagctcttc gcgttcgatc ttcaggtgta
     3721 tttcagcgga gatgctcggg ctgatgttgc acagcttggt gactgccgcg acggcaagcg
     3781 gagcccgttt tacgatcttg tttgccagtt cgagtgcggc gtccacaaca tttccttcgg
     3841 tgatgtggtc gacaaggccg atagagagag cttcatccgc ttcacactgc ttgccgagat
     3901 agatcatttc cagggctttc gatttgccga cgagccttgg caggcgcaga cttccgccgt
     3961 agccgggcat gatgcccaga ttcgtctcgg tcaggcctat gcgagcgcct ttcttcatga
     4021 agcgtatatg acaggccata gccagttcgc acccgccgcc cagggcgtgt ccattgattg
     4081 ccgcgatgac cggtttcgga aaatattcaa ctttgttcca gatgtcctga ccgcgtttca
     4141 ggaagtcgac ggcgctgaaa tcaccgaacc cgctcgcaag atcggcgccc gcgctgaagg
     4201 ccttttcgcc cgcgccggtg atgatgacgc atcggatggc tttgtcggct tcaacggcgt
     4261 cgagatgctt ccgcatggct tcgaaaactt ccccgctcaa ctggttcatc ttcggtttgt
     4321 cgatcgctaa tatggctact ccgtctctta tttcttttgt tactgcttca cccatgatga
     4381 tcctccttgt gtgatattgt ttggttgtgc tcggccggtt agccgggcga actggtaatc
     4441 aattatcttc cgatcatttt gaacgagccg ttaatcttgc cgcgcccgga cttcaggaaa
     4501 tcgaaacctt ccctgacctt ttccccgtcg tgatgcagag tcgagccatt ccgcgtctca
     4561 atacgtccgg tcagagaaaa cgattttctt tatgtcattg atgttgatta cagcgtggtc
     4621 gagtataaga gacaggtggt tgtgtgtcaa ggggattttg gtccgttatt gtgaaactca
     4681 aatttcaaaa acgaagggct ttgaaatttg cgggtcgaac aatcccgccg taactggagg
     4741 ggaatgagat ccgagtttca gaaacagccg gccctggaac ttgcagattg aattatcctg
     4801 cataggtatg gggataatac tccgcctttg gggcggaagt atagtccagg acttcccttg
     4861 gagggctacg tgattttgct tgaaggggag gcaaaaaaat gattacatca ttcccacaaa
     4921 caaaaaatca ggagggtcaa gatggcttac gataatatta tcgtcgaaac aaaagatttt
     4981 gtaacggttg tcacactaaa tcgtccgccg atgaattcca tcaatctggg cattcgtgaa
     5041 gaattggatc agatcataac cgacgtacag accagcaagg agacccgggt tgtcgttgtg
     5101 accggttccg gagaaaaggc tttttgcgcg ggaatggatg ttgccgatgt tgccaacatt
     5161 gacaaaggac ccaacggcat agacgtatgc aataaagtag aacgctccct aaaacccgtt
     5221 atcgccgtca tcaacggcca cgctctgggc ggaggatgtg agctggccat ggcctgccat
     5281 tttcgattta tgacagacaa tcccaaatcc cagatcggat gtccggaatt gaatctggcg
     5341 ctgacccccg gctggggagg catacagaga atgcccagaa tcatcggcaa atccaaagct
     5401 ctggatttta ttttattcag caaaaaagtc agcggtcccg acgctctggc gatcggcctt
     5461 gtggataagg ttttccctgc ggccgattta atgaaggagg ccatgcactt cgcgcaggcg
     5521 ctttccaaaa gagcgcctat agccgtaaat gcagtactta acggaatgaa catcggtttg
     5581 gaaaaaggca ttgttgcggg tctggaagcc gacaagcagt ggatcgaaaa actcaaaacc
     5641 agtaaggatg cgacggaagg cgtggtcgct ttcattgaaa aacgcgaacc gaattttgta
     5701 ggcgaataaa atccgattga cgtgcctcgc ggcggcatgt ctccgccgtc cgcgggagat
     5761 cctgccgttt gcactgccga cgtcgcttta cgctttgacc ggtgttattc aaatctgaat
     5821 ctctccagag atttttaagc ctccgttttc tttatacgaa aacggaggct tgttattgat
     5881 gatcctcaat gcgtatgcag gattttgcga tttttgttca gcgcattgtt cgaggcgatc
     5941 tgttatgtcg actttactga agcaaggggc ccatgagctc aaaaattccc atcgaatcat
     6001 acaatggctt ggcgcctgtc agatcggaaa tcttcgcaaa gttgtcgcgc acttcttcag
     6061 cgggaatatc gcgcgatccg tctcccaggc atgcgccggt cgcacataca atagccgttt
     6121 ttgcgtacca gccagccccc atcgtaaaaa tggagccgtt ttcctggcat tcttccgaac
     6181 agagataaac gaccatgggg acattgaatt tcgcctgcag cttcggagcc agctctttcg
     6241 gaatcaggtt ctccgtcatc cgggaatagg cagtgggcgc aatggcattg acgtggacat
     6301 tgaacttcga cgcttccagt ttaaggacat tcataaaacc caccaggccc atcttggcag
     6361 agccgtaatt aatctggccg aagtttccaa agatccctga tgtggaagag gtgaccatga
     6421 ttcgtccgta attgtgctcg cgcattaccg ccaacgccgg ttgagtcaca cagaacgctc
     6481 cccggagatg gacgctcatg attgcgtccc actcgtcggc ggtcattttc atcatcgatt
     6541 tatctcttaa aaaaccggca ttgttcacaa gaacgtcgac cgtgccgaac gtatccactg
     6601 cggtcttgac aatgttaatg cccccttcag gcgttgccac gctgtcgtaa ttggcaaccg
     6661 cttttccgcc ggagtttttt atttcggtaa ccaccgcatc ggcggcgttt tttgttgatc
     6721 ccgatccgtc gcgggcaacg cccaggtcgt taacgacaac cgaagcgccc cttctggcaa
     6781 attccagtgc atattcgcgg ccaagaccgg atccggcgcc ggtcacaata acgactttac
     6841 cttcaaaact tatgttactc attgcattct cctttttatt ttttctaaac tcttttgggt
     6901 aacttatgct tcaggattat atactgtttt gtgtaatttg accggtttca tttgcttatt
     6961 gcgcatgcgc agattgagca tttccacgcc cacggaaaac gccatggcaa agtaaatata
     7021 ccctttgggc acatggacgt caaatccttc cgccaccagg gtcacgccga tcagaagcag
     7081 gaaagataaa gccagaattt tgatggtcgg gtgggcgtcc acaaatttgc tgatcggttt
     7141 ggcggcaaag agcatgacgc cgaccgcaca aatgatggcg atggccatga tggaaacgtc
     7201 ctttgccaga ccgaccgccg tgatgacgga atcgagagaa aagacaatat ccagcagggc
     7261 aatctgaatc agaaccgctg ccacactgga tgaaataagt ctgggagtct cctcctgagc
     7321 gccctccagg cttgtgtgaa tttcatgcgt ggccttggcc agcagaaaga gtcctccgcc
     7381 gatcagaatc atgtcccgtc cggaaatttc ctggccgaat accgtaaacc agggcgcggt
     7441 cagtttgata acccagaaga tggaaaacag aagcgccagt cgtgtcaaca tggccagcat
     7501 cagccctgtg tttcttgcaa aattctgttt gtttttcggc agccggctga ccagaataga
     7561 aataaagatg atattgtcga tgcccagaac aatttccaat gccgtcagcg tcgccagagc
     7621 gatccacgct tccggactgg ttagccatgc aaacatattt gtcctccgtt aattttaaag
     7681 atgaggtgtt taatattcta ttcccgcgcg gtctttgata ccggcattgt agtgatgctt
     7741 gatttcctga acgtcgctga cggtatcggc gagcttgatg atttccttcg gtgcgtagcg
     7801 gcccgttaaa atcagatcca catgagcggg cttgtttttt ataagtttca tcacgtcttt
     7861 taaatcgaca agtttaaaat ccagcgccac attgatttcg tcgagaatga ccatgtcgta
     7921 tttaccgctc ataatggcct tgcgggcgat atccagtcct tttcgggcca tgtcaatatc
     7981 gatggccgcg ggatgatcgc gcatgacaaa actgtccagg ccggacattt tgatcgtgat
     8041 gttcgataaa tatttttccg cggcaataaa ttcgccgtat tttctcccct tcataaattg
     8101 cagaacaaaa accttatagc cctggccgat ggcccgcagg gcctgtccga acgcggaggt
     8161 tgttttcccc ttgccgttgc cggtgatcac gatgaccaat cccttttgtt tcgggacggc
     8221 attttccttc gatgttttta aacccgaaga tttcttgctg cgcacgttgt aaacagcggg
     8281 gtcttccgtt ttaaccatgg tcattttttc acctgttttt gaaaaaattt ttccgtgatg
     8341 tcttccgcca cggtataggg atcggatttc ctgttcttta ttttctcgga cagttttccc
     8401 atctcgccgc tgctgatcag ggattgcatc aggggttcca tgaggcaggc gccgagcgcg
     8461 tctttaattt cggccatgac tttgcgatgc attctttcgg tgagttggcc gctttgttcc
     8521 aggaaacaat gatgttcttc aattttccgc gccagttcgg cggtgttttc gtcaaaggtt
     8581 gcccgttcaa acgcatcgcc caccaggacg atcggcggct tccagccgtc agcggttttc
     8641 ggggataaat tgagcatgga agacagttct gtctgcaact gtccggttcc ggggcggttg
     8701 gccttgttga tgacaaagac atccgctatc tccatcaatc cggccttcat ggtttgaatc
     8761 tcgtcgccca tcccgggaac cagaacgacc accacgctgt gggcgtgatt gataatatca
     8821 atttcctgct ggccgatgcc gaccgtctcg acgataatga tatccttgcc catggcttca
     8881 atcacatgga tggcgttgcc ggcggcctga gagagccctc ccagagcgcc gcgggtggcc
     8941 agcgaccgga caaaaacgcc gtcatctgcc gcatggcgct gcatgcggat ccggtcgccc
     9001 aggatggcgc cgccggtgaa ggggctggtc ggatcgacgg ccagcacgcc gacggttttg
     9061 ttcagttttc tgaattcgga aatcagcgcg tcggtgagcg tgcttttacc cgcgccgggg
     9121 gcgccggtaa ttccgataat gaaagatttg ccggtgtggg cgtagagttt tttgattttt
     9181 accttggcat cgggacgctt gtcttcgatg tctctgatca ggcgcgaagc cgcacgaaca
     9241 tcgcccgcgc agattttttt aatggtggca tccataaagc ttcaagagaa tttctagtgc
     9301 gttttgatat tttttttaat ccattccaca atggaatcga gtgctgttcc gggagtgaaa
     9361 atcgctttga tgcccgattc ataaagcatg ggaacgtcct gttccggaat gatgccgccg
     9421 ccgatgacgg cgatatcccc ggcgcctttt tcacgcagaa gttccacaac ccgggggaaa
     9481 agggcgcgat gtgcgccgga aagacagctc aatccgacca ggtcgacatc ttcctggatg
     9541 gcggatgcgg cgatttgttc cggggtctga tggcagccgg tatagaccac ttcaaaaccg
     9601 gcatcgcgga aacagcgggc aagaattctg gcaccgcggt cgtgtccgtc gaggcccggt
     9661 ttggcaacca ttacgcgaat ttttctttcc gacatattac tatctcctta aattatctct
     9721 aataccaagt tgcattcatc aggataccgc ggcggcgagg aggcaccgga ggcgcattac
     9781 taatacgtcg aggacgccga cgacgatgcc aacaaaggta gccgtatgag ggcggcttgg
     9841 ttttaataca gtcccgggtc cctatattcc ccgtaaacgt cgcgatagac atcgcatatt
     9901 tcctgcaggg ttgccagatt tttcaccgcg tcgatgcagc aaggcatgac gttattcccc
     9961 tttttacagg cgtttctgac gtcttcaaga gattttttca aggctttgct gtcgcgcttg
    10021 cggcgcacac tcttgagcct cttgatttgt tcttcgccca ccttttcatc aatatcaaca
    10081 agcggaagcg ggtgcgtgac gtcggtggca tatttgttca cccctaccat ggatttctcg
    10141 cccgcatcca actgcttttg aaacctgtaa gccgcgtcgg atatctccat ctgggggaaa
    10201 cctttttcaa tggcggtaat cataccgccc attgcatcaa tcttgtgaat atagtcccac
    10261 gccttttctt ccatttcgtt ggtgagagac tcgatgaaat aagagccggc caggggatca
    10321 atggtgttgg ccacgccggt ttcttcggcc agaatctgct gcgtgcgcaa cgcgatttcc
    10381 acggcatgct cggaaggaag acagagcact tcatccagcg aatttgtgtg caacgattgt
    10441 gtgccgccca agaccgctgc cagagcttcc acggtggtcc ggataacatt attgtacggc
    10501 tgttgagccg tcaacgaaca gccggccgtc tgcgtgtgaa agcgcagcca cagggaacgg
    10561 ggatctttgg catgaaaacg gtcccgcata atcttcgccc acatgcggcg ggccgcgcgc
    10621 attttgcata tctcttcaaa aaaatcgata tgcgaattaa aaaagaagga cagccgcgac
    10681 gcaaagtcgt ccacattcat ttttttacgg cgaatcacgt catccacata ttcgatgccg
    10741 tcacggatgg tgaacgccag ttcctggacg gccgtggctc ccgcttcgcg gatgtgatag
    10801 ccgctgatgc tgatggtgtt ccagcgcgga acatattttg tgccgaattc cacggtgtcg
    10861 ctgatgagtt tgacggaagg ttccgggggg cacatgaatg ttttctgtgc gataaattcc
    10921 ttaagcatgt cgttttggat ggtgccgccg attttggaaa gcggtatccc cttgatatct
    10981 gcagccgcgc agtacatggc ccataaaacg gttgccggag ggttgatggt catggaggtg
    11041 gtgactttat ccatggggat gccttccatg agggtcagga aatcttccag ggtgtcgatg
    11101 gcgacgccgc atttgcccac ctcgcccagc gctttgggcg agtcgctgtc atagcccatc
    11161 agtgtcggaa aatcgaaggc cgtactcaat ccggtttgcc cttcgcgcag cagcatatgc
    11221 cagcgtcgat tggtatcctg ggcgctgccc atgccggaaa acatgcggaa tgtccagtaa
    11281 cgtccccgat agccggtaac ctgattcccg cgcaaataag gaaattcacc gggaacgccg
    11341 atgtcttcgg tataattgat gtctttgata tcttccgggg tataaagcct tttaatttcc
    11401 gtgtccgaca cggtggaaaa acgttttttt tgttcaggat tattttttaa cgtcttttgg
    11461 acttcctttt cccatttctg ggtcagtttt aaagacgcct cctgcatttc ttcggagaaa
    11521 tacacgatca agtcctcccg tatggcgcgg aatgattagg aatattcgta aaaaaactta
    11581 aagccactaa ctcaaatttt ttgtttttgt caagacatat tgaagcaatt cacagcaaac
    11641 cacaggaaat ctttcattgt tccgtgaatc aaaaaagcac ccgcatcaat ctctacagcg
    11701 ggtgcttttg ttggcgaatc ctgtgtttat aagattatat gggtttaacg ttttctgctt
    11761 ccaacccttt tttgccttgc tggatatcga atgatacccg ctggccttca ttcagcgttt
    11821 taaagccttc gacctgaatc gcgctgaaat gaacgaaaac atctccgccc tcatcctgtt
    11881 ggataaaacc gaaacccttg ttggcgttga accactttac tttcccttct ggcatagttg
    11941 cacaaactcc tttcgtaaat tttccaagtc gaattaacac tatgacagaa ttgcaacctt
    12001 aaaaccttta aagataaatg agaatctctc cggcaggata aatacacact atgctttaaa
    12061 gaatattaca gattaacctt gtacttcaaa atgtttaaat tcaacttttt tctcagcaac
    12121 atattgtttg ctgcagtggc cctatttaat cttaacatcc atcaaagtca agctatattt
    12181 catgggattc gccggagacg ggccaggcga cgtcggatgc ttaaaccgcc gcactttgag
    12241 ggtttaagca agatcgtgct taatattttt cccggaatgt gctattaaaa acacaattca
    12301 agccgacaat ggattgtcgg ccaaaggagt gaatcatgaa aattaaagcg tccgtcctga
    12361 ttgtactgct ggctgcgttg tggatgacgg tgtcggcagg agcggctgaa gtgaaacccc
    12421 ctcaggtggg gcaggggttt cccgatgttg aattgtccaa accggtgatc gccggtgaac
    12481 tgaaatatct cggactgtcc ggcagcggat cttttagaat caatcagatc aaggcggatg
    12541 tagtgattgt agaaatattc agcatgtatt gcccgcattg tcaggcggaa gctcccgccg
    12601 tcaacgagct ttatcagctc attgaagaaa atgcggcgct taaaggaaaa attaaaatca
    12661 tcggaatcgg cacgggcaac tccatctatg aaaccgatat tttcaagaag aaatacagcg
    12721 tgaagtttcc tctggttccc gatggagatt tcaagatcca taaaatcatg ggagaggtca
    12781 gaacgcccta tttcattgtt gttaaattaa aaggaaagaa ggcggacgtg atttattcca
    12841 aagcgggcgc tttaggagat aacaaaactt tcctgaaaga gattattaaa tcagcgggat
    12901 tgaaatagga ggcggccatg aaaaaaaaga tcattttggc agcgttgttg atttgttttc
    12961 ttgcacccgg ggctttcgcc ttgtccgatg tttttaaaaa taatgtttat gaaacaggcg
    13021 aactgaagcc tcaggacagt gtattgaaag tcaaggttgg ccagcccgcg cccgatttca
    13081 cgctgaaagc cgtcagcggc aaaacgattt ctctgaaaga ctaccgcgga aagaaaaatg
    13141 ttgtgatttc ctttgtgccg gctgcctgga cgccggtgtg ttccgatcaa tggccgggct
    13201 acaacatcgt gaaagacatc tttgacgtca atgacgccgt tttactgggc atttctgttg
    13261 acaacattcc cacgctgtat tcctggacaa accagatggg ccatttgtgg tttgatgtcc
    13321 tgtctgattt ctggccgcac ggtgccgtgg cggatcaatt cggcgtactg cgctccgacg
    13381 gcatttcgga gagagccatt ttgatcatcg ataaaaaaag tatcatccgt tacatcgatg
    13441 tccacaacat caataagcgt ccgccgctgg agagcattgt ccgggaactg gaaaaattaa
    13501 attaatccat agaattatca ttttcaggat aagccccgtt tcaacgcgga tgtttttcct
    13561 tgacacatcc ccgatattaa tttatgcacc ttccaaatgt ttatgaacgc cggtcatttt
    13621 ttgtttggcc ttgattttac gcgaatgatt tgaaaaggtg atcaggttcg acgtggaaga
    13681 aaaaaaaaga aacaggaaac gaaaagaaaa ggaaaataga cgcagtacga tactcaaagc
    13741 cgcccgaaaa ctctttttag aaaggggttt caaagctgtt acggttgaca gtattgctga
    13801 aaaggcggaa gtcagcaagg gatccattta tctttgcttt gaaagcaaag aagaaatata
    13861 cacacagatt ttaattgccg acaacatttc cctcaacgaa cgcattcaga atttttccct
    13921 gattgaagcc tcggcctccc agcttctgct ggagtttgcc cgtatttatg tcgatttctt
    13981 tctcagcgac aaggaacttt tcagaattct gatgacgttc atgatgcaaa cggggcagat
    14041 gcacttgacg cagcagcaaa gcgatgagct gatccggtcg acgaatgaaa acatcaaagt
    14101 tatttcggaa attattcaaa aaggagtcga ttccggtgaa ttcagaccgc tggacaatat
    14161 cttgcagatg cagaacgcca tctggggaat gttgaacggt gttatttcac ttttcctttt
    14221 caccggcaat ccgatcaaaa gaatggagag aattcactct accgttcagg aaagcatgaa
    14281 tgttttcatt agggggttga aggcgtaatt tttccggaat gcaaggtgcg caatccgaat
    14341 gccttgcggt tcagtgcagg gcttgaaccg ggtatttcgg gtttacttcc ccatggtttg
    14401 aaataatttt ttaaattcct gctcggcctc ttttaatatg ttttccacat ctttttccag
    14461 tttctcgaat ctttcgataa tggttctcgc ctgagccgtc agttgcaggg ctttttcttt
    14521 ttcgctggtt tccaccagtt ttttttccat gcgttcttcc gacgctttca gtctgcccca
    14581 ggccgcacgg tatgacattc ccagttcctt ggcggcggca ttgaggctgt gctgctgttc
    14641 gatggatttg agaatttcat cgcggccttt gccgaaaatg accttgccgt tttcttcaat
    14701 ccagatttta tatttgattt ccatcggtgc ctccccccca ggctattgaa tcttgttttg
    14761 ttataattaa catacttaca atatgtcaat aaaattcgca accgccacag cgccgtaaaa
    14821 cgtaaactgt gaaccggttg aagaattgga gaagaagaca gatgctctca ggccggcata
    14881 aatcatcttg actattcaat gacgttgcct gtatttacat aaaaaaatca ctgaaggagt
    14941 tgatcgtatg cccggactgg ttcgatttgg ggtatcgctg gaagaagatt tgctggttaa
    15001 attcgaccgg catatcaagc agaagaaata caccaaccgt tccgaagcca tccgcgacat
    15061 gatccgcgag gaactggtcc ggaaagaatg gacggaaaac aaggaagtga ccggcgcaat
    15121 cacgctggtt tatgatcatc acaccaggga attagtcacc aaagtgcttg atgtgcagca
    15181 tgattatcat acctgcattc tttccacgca gcatattcat ctcgatcatc ataattgttt
    15241 tgaaattgtt gtgacccggg gcaagtccaa agaaattgag gagctctttc agaaactcaa
    15301 atcgatcaaa agcgtcaaac atgccggatt catgatggca accaagggga aggatttacc
    15361 ctgatgcgtt taaaggggtt atcgcttctg catggataag ggcagttcca gattattttc
    15421 ttccagcagt tcctgatggg tgaggatctc cgaaaccggg ccgtcggcgg ccacccttcc
    15481 ttctttaatg acaatacacc gatgacagac atccagaatc agatccagat catgtgaggc
    15541 gatgatcttc gaatgctcga aggttttcag cagattgatc agcaggcgcc gcgaccgggg
    15601 atcgagattg gccgccggtt cgtccatggc cagaatatcc ggctccatgg ccatgacgga
    15661 cgcaatggcg acggcgctct tctggccgca ggagagatga tgaggaggtt tgtttttcag
    15721 gtccaggctg ttgaccagat gcagggcgaa ttccacccgt tcccgcacgg cggtttcatc
    15781 caagcccagg tttagcggtc cgaaagccac gtcttcatac acggtcggca taaagagttg
    15841 atcgtcggga ttttgaaaaa cgataccgac ctttctgcgg atttccggtt gcgtcttctt
    15901 cgtcagataa acatcgccga tattcaccgt gcccgatgtc ggcatcaggt agccgttgag
    15961 gtgcatcagc agcgtagatt tgcccgcacc gttggcgccg atgattccca ccgactcccc
    16021 gtgcgtgatc cggaaagaaa tatcctggag ggcttgggtt ccgtccggat agcggaaaga
    16081 aacattttta aactcgacga tatggtgact catgaaaaaa ctccttgtgc aaaccggccc
    16141 agaatttcgg ttgccggaca gaaccggaag atcaacagaa acgccgcaac cgtcgcggca
    16201 aaaatcatgt cgccggtttt gaactgaaaa tgtttgagcg cgggcatatc tccctgaaag
    16261 ccccgggaca acatggcgtt atagacccgt tccgcgcgct gcaccgtccg caaaaaaagc
    16321 gttcccgcaa tgcggacaaa gattttgatg cccttgcctc ggtctccgaa agagcgcata
    16381 tttctggccc ggacaatccg catggtttcc tccatcagga caaaaatata gcgataaaga
    16441 aaaagaagct gtgaaacaaa aaccgaaggc agccccaact gcctcaacgc gtggcagaca
    16501 ccgggaaaag aggtggtggc gacaagcagc agagccgtgc tgacggtaag cgtgaacttg
    16561 agcaggatgg aaataaaaga cagccatcct gccgaaatcg tatatccgtt gactatcgat
    16621 atctgtcccg aatccagcag cggattgaag atgccgataa aaacggcgaa aggtgaaacg
    16681 agaaggactt ttttaaagat aaacataaac ggaatctcgc ccagcgtcat cagaagcacc
    16741 gggaaaagaa aaaaaggcag cagagcaaaa atttcgtatt tggaaaagga aatgaccgta
    16801 agcagaaaaa gcagggtaac acatagtttg acccgtggat ccagccggtg cacaaaggtg
    16861 ctcttgtagg aaagctgatc gaggaagccg atattatagt attgaatgtc aaatgacatg
    16921 atttggcccg ataatggctt taagaattga ttctagatat catacgccct gacaggaaat
    16981 ccagctattt tcaaggcgta tgccgggtat gatgacaaaa gttattgttt tttaaaaagc
    17041 cgtatgccga aaccgatgaa aatgatgaac atcaacacaa tggccgatcc gacaactccg
    17101 gcgacagatg ttccgggttc aatgcccggc cagaccgagg gcgactcctc cgtgttttgt
    17161 tcgtccgcgg gcggtttgaa accataatct gggagaaatg ccgtcttttc ctgaatatct
    17221 ttgaggacag gcgcaatgcc ctgtgttgcc tcttccagct cgcccttgcc ggtaactttc
    17281 tcaatggacc actccagccc gtcgggatgc gttgaggcaa accaggaaag cgcaccgccg
    17341 gtaatgaccg ccagaatcac aaatgctgca agaatgttct tgatggaaag accggcatcc
    17401 aggggacggg cggcggcatg ggcatgcaga atttccggcc gggcctgttg cacatacagg
    17461 atgatgccgg ccgtgatgat tccctccacc aggccgatgg ccagatgaat cggctgcatc
    17521 agcaggacaa acgtgccgaa ggggagttcg ctgattccgg agaatagcgt ttccagaacc
    17581 accgaaaaag cgcccaactg cagggcaacg attgcgccaa gtattgcggc aacggatacc
    17641 cgtctggcgc tttttccgtc tccggcaaca ggtttgtaaa tcaggggata aaccagaaag
    17701 cagggataga tgcccatgtt ccagatatta cagccaagcg cgagtagccc gccgtcggca
    17761 aagaacagcg cctgaaccgt caatatcgag gccatggcca gaaaagcggc gtagggaccc
    17821 agaagaacag ccaaaagcat accgcctccg atatgtccgc tggaacctgt gacgggaatg
    17881 gtgaagttga tcatctgcgc cgcaaagaca aacgcgccca tgacgcccat cagcgggatg
    17941 gttttgttat cgatattttc tttgagcttt tttgatgaat atccgatggc ggccacggaa
    18001 ccggcccaca tcgttgctcc caccgcgggt gagagtaaag catccgccat atgcatgtta
    18061 aattctcctt ttaaaaaaga tcagttatcc gccttcctct ctagaaccgg aatgcaagtc
    18121 cggcgagcgc agaaatatcg gcttccgagt ctgtcaaacc gtatttcacg ccgaggtcga
    18181 tgtccaaatt ttctgcgatc gaataaataa tcccgccgat aagaaacgca ggatcgtcat
    18241 ccgcttcgcc atcaggattg cgctcaacgc cgatattggc gaccagactt aagtctttga
    18301 caatttccca ggcagcggca acggaggcat gccagatatt ctcgcgctcg tcggcgtcgt
    18361 tttcatttct gatgtatccc agattaacat gaaacgcgaa cggttccact tcctgggatg
    18421 caatgaaaaa aatctgacct ccaaattttc ctgtgcccag tcccttgtca tcatcgcccg
    18481 tggggaaact gattcccggt ttcaacgcca ggctgaagcc ttctttttca aagaccttga
    18541 tttttgcttc taatgaagca tcgccgatgc cgtcttcctt atatacaagg ctgccatttt
    18601 ctttttctct tccccaaaga taaggcaggc ttaataccag atcaatatta tctatcatcc
    18661 cgtaagacaa tgtggctgcc gtctcaactc ccgttgcttt aatcgaaaca ccaccgacat
    18721 tttctttgtc ctgatcatat tgtccggtca attccaattg aaaatttccc ttcccctgcg
    18781 tgccggtgtc gtcggtaatc aggggatgcg ccgcccagga tggaccggcg atgaaaaaca
    18841 gcactgccca taccaatccg tttataaaat gttttctgat cattttctcc ttccatttgt
    18901 gctacgatta taattttagt ggcacgatat ggcggttctg tcaagctttt aatgatttta
    18961 aaaagcaata aaccctatta aaccggcttc attaaaatat gttttatata acatattgac
    19021 ttttgtatga ctttcggtca atctatgcca cgtttgacat ataccaaaac tgacataggt
    19081 agtggggagg attttatgca tatttcaaga agaggttttt taactctttc cagtgccgtc
    19141 ggaggcggga ttgccctgtc cagcttgggg cttaatctga agccgacgcg ggcatatgcg
    19201 gacgagttag acaagatgaa cagagtgaag gccgccaaac agacgacgac ggtttgctgt
    19261 tactgttccg tcggctgcgg tctggtttgc agcacggaca aggcgaccgg aaaaatcatt
    19321 aacattgaag gcgacgccga gcatcccatc aatgaaggat cgctttgcgc caaaggtgcc
    19381 ggtttcttcg atctgaccga agccaacaag cacaggctaa aaaaggttct ctatcgcaag
    19441 ccgtatggaa cggaatggga agagaaagac tggaactggg cgctgcccag gattgcccgc
    19501 ttgattaaag acactcgtga caaaggtttt gtaacgaaga acgccaaggg cgaactggtc
    19561 aatcgctgcg aaacaatcgg ccactacggc agttccaacg tagataatga agaatgttgg
    19621 ctgatgagcg tcaaatgccg tgctctgggt ttggtttata tcgatcacca ggccagggtc
    19681 tgacacagcc catctgtagc ggctctggga gagtcgttcg gacgcggttc catgacgaac
    19741 cactactgtg atttgaaaaa cgcggatgtc attttgatta tgggcagcaa tgccgcggaa
    19801 catcacccca tcgctttcaa gtggattatg agagcaaaag aaaagggagc gacggttatc
    19861 catgtcgatc cccgttatac aagaacatcg gcgcgctgcg atttccatgt gccgcttcgc
    19921 tccggtaccg atatcgcttt tctgggcggt atgatcaaat atattctgga aaacaataaa
    19981 tacttcaaag actacgtagt caattacacc aacgcgtcgt tcctcgtaaa caaagatttt
    20041 tcttttaatg acggtctttt ctccggttac gattcgcaaa aacgcaagta caataaagat
    20101 acatggactc tcgataaaga tgcaaacggt attcccaaac gggacatgac cctgtctgat
    20161 ccccgctgtg ttttccagat gctgaaaaaa cattattcac ggtataccct ggataaggtt
    20221 tccagcatca ccggcgtatc caaggacaac ctcctcaagg tgtacaaagc ctacagcgcg
    20281 accggcgctc cggacaaagc cggtacggaa tgctacgctc tggggtggac acatcatacc
    20341 gtgggcgtac agaacatccg ctccatggcg attattcagt tgcttttggg caatatgggt
    20401 atcgcaggcg gcggcatcaa tgcgctgcgc ggcgaaccca acgttcaggg ctcgacagac
    20461 cattgcattt tatatgcaaa tctgcccggt tatctgaaga tgcccgcggc ttctctggac
    20521 acgctggaac actatttgaa aaaatgcacg ccggaatcca aggatccgca gtcggccaat
    20581 tactattcta attaccccaa attctttgtc agtttcttaa aatccctgtg ggacgacaag
    20641 gtgacaaaag aaaatgattt cggctacgac tggcttccca aaattgatga cgggaaacac
    20701 tattccacga tgcacatgtt cgatgcgatg tatgaaggca agatcaaggg atacttctgt
    20761 gttggcgcgg atacggccgt cagcacgccg aacagcaaca aggttcgcaa ggcgatggaa
    20821 aatctggact ggctcgtggg tgaaaatatt ttcaacaacg aaacctacga attctggaga
    20881 ggccccggtg tcgatcctgc aaagatcaag acggaatgct tcctgcttcc cgcttccgcg
    20941 agtatggaaa aggaaggatc gcagagcaat tccggccgct gggttcagtg gaaatacaaa
    21001 gctgccgaag ctcccggcga tgccattccc gtgggtgaaa tcgaaattaa gattatggct
    21061 gaagtcaaaa agctttacca gaaagaaggc ggtcccaatg cctcggctat taccaatctc
    21121 aagtgggatt atctggacag caaggggcac atggatgtgt tgaaagtttc ccatcaggtc
    21181 aatggcctct ttacaaaaga cacggttatc gaggataagg cgaaaggtac atccactttg
    21241 tttaaaaaag gacagtgtgt cccgaccttt ggaaacctca aggatgacgg atccacagcc
    21301 tgcggtaact ggattatggc cggtagtttt gacgaaaaag gtttcaacaa aatgaccaaa
    21361 cgcggcaaag acgatcccac cggtttgggg cttttcccca actggtcatt cgcctggccg
    21421 gtcaatcgcc gcatcattta caatcgcgct tcctgcgata tttacggcaa accgtacaat
    21481 cccaaacgta atattctgga atgggtggga gataaatggg ttggagacgt gcctgatggt
    21541 ccctggccgc cgcaagcgga caaggcaaaa ggcaaatatc cttttattat gaaacaagat
    21601 ggtgtggcat ctttgttcgg acccgccatg gctgaaggtc ctttcccgga gcattacgaa
    21661 ccgctggaaa gccctctggc taaaaatccg atgtccggac agctcaacaa cccggccatt
    21721 gaaatcttca aaggtgaaat ggataaagtg gccagcgcca gcgagaagtt cccctatgtc
    21781 tgcacgacgt attcctgcac cgagcactgg tgcaccggcg cgctgacccg ttggcaggca
    21841 tggcttctgg aaatgcagcc ggagctctac gttgaaatcg gcgataaact ggctaaggaa
    21901 aaaggtatta aaaatggcga ccgcgttaag gtttcctcca ttcgtggcga agcgccctgc
    21961 gtggccatgg tgaccaaacg cttcaagcct tttacggttg aaggaaaaac ggtccatcag
    22021 gtcggcatgc cctttaatta cggctggctc ttccccaagg acagcaccag tgacagcacg
    22081 aatgtattaa ccccaaccgt tggtgacgcg aatgtcttct gtcccgagta caaggcattc
    22141 atggtcaacg tggaaaaggc ttaaggagga taaaccatgg gaaatgaact tgtaaaattg
    22201 attgatacaa ccctgtgcac cgcctgccgc ggttgccagg tcgcctgcaa gcagtggaat
    22261 gaaatgccgg gcttcaggac aaaacaaacc ggcacgtacc agaatccgcc ggatttgcag
    22321 tggaacacct ggacgctgat ccgctttcag gaatatgaag acaaggatga aaacttccag
    22381 tggatcttcc gcaaagacgg ttgcatgcac tgcacggacg catcctgtgt aaaagtctgc
    22441 cccagcggcg ctctgtatta tacggacatc aagacggtgg gtattcatcg cgacaaatgc
    22501 atcggctgca aggaatgcat tgcggcctgt ccgtttgaca ttccccgcta cgataagaaa
    22561 accgagaaga tttacaaatg cgatctttgc tattcgcgca ttaaggaaaa ccttcccccg
    22621 gcctgtgtca aatcctgtcc gaccggcgct ctgaccatcg ggcccaagga cgagatgatt
    22681 aaaaaggcct atgcccgcgt acagcaactg ggcggcgacg ccaatgtgta cggcgataaa
    22741 ttcgtaggcg gcacacacgt tatttatgtg cttcagttca agccggaagt ttatgacgaa
    22801 ctgccgcaag atccgaaagt accggtttct acctttatct ggaaagatgt gctcaaacct
    22861 ctgagcctgc tggccgcggg cggcgtgatt ggcggatcat tattacatta cttcacccac
    22921 ggtccgcaca agccccatcc ggagcattat gatcaagata cgaagcaggg aggtgcgtaa
    22981 gatgaaaagt aaaatggtaa tggtgacatc aaaatatgag agattcgttc attggctgtt
    23041 ggccatctcc tgccttcttt tatgctatac cggactgggg attatgtaca aagagttgaa
    23101 ctttttgggt ttcattttcg gcggtttgaa aggattggcc accgttcatg acattatggc
    23161 tgtggttttt gccatatcgt tggtattggc catagcgatg tggtggaaag aagcgggcat
    23221 atttgttttc ccggaagact gggaatggat caaggcagcc ggcggttacc tgtggcatct
    23281 ggatcacgtc ccggagaccg gcaaatacaa tcccggccag aaggcgttct ttctgaccgt
    23341 cgccttgttt ggattactga tgatctgcac cggatttttc atgtggtatc cccacgtact
    23401 tccggcggca ttgatggatt gggtttatgt cctgcatgtg ctgggttttg tggtcatctt
    23461 tgcgttcttc tttgttcacc tgtatttggg gaccattggt aatcccggat ctgtttccgc
    23521 gatgatcagc gggaagatgg agctgccggt attgagaatg cttcatccga aatgggtcaa
    23581 agagatggag catgaaggca aactcatgat tgccgacgat aaaaagtaag attgaacaat
    23641 ttcccgcaag gaaacgatgt ggttcatatt taattgaccg ggtggcgcac atctgataga
    23701 aggtgtgcgc cacttttgtt ttgaggctcg cttttgtgag attcgttgac catcgatagt
    23761 agcttttggc tatcggaaac aatcccgcat gaaacactat gggggactaa ttagtaaaag
    23821 gaaaaatcat gacccaagaa caaacgtccg agatcaaaaa tatcattgac cgcgctctgg
    23881 aacagaatcc tgagaataag gaaatcatta aagcgtttgc accgattatc atccggcaga
    23941 gacagcttgc cgaaaagcct tcagagcaga aactggactg cttggcaatc gataaacaga
    24001 aactcaaggc cggcgtgccg gtcagccgtc agatcaattt aatattgccg ggcgatccgt
    24061 tgaaagaaat cgctctgtcg ctggccgagg cggtgaagga aggcatgccg caactgacgg
    24121 aaaatatcga tctgatagca gggctgatca aagacgataa aatcaaaccg gcggattatt
    24181 tcaaaagcac cccggacggg gaaagcatgg ccaccgatgt ctgggcgaaa gatttaaaag
    24241 tctcgccgtc caatgcgtca tttttaatga gcctggtcag ccgcgttatc ctggaacaga
    24301 gagccaacga gataacggcc gccatcggcg cctttgactg ggaaaaaggg tactgcccga
    24361 tttgcggaga atttccgtcc gttgcattaa ttgaggaaga gggcggcaag cgcttcctgc
    24421 attgttccgc ctgcggttat tactggcgct tcacgcggat cagctgtcct tactgtgaaa
    24481 aggaaacccg gcaggggatg gaatatttct atgtggaaaa taaaaaacag gaatccgctt
    24541 tcgtctgtga ccaatgtaaa aaataccttg tcacccttta ccgggcgggc caggtctttg
    24601 cgcgcgatat ggatgtttcc gccatcagcc tgattcacat ggatctgctc atgcagaaca
    24661 aaggctacga acccatgacc gcctgtacct ggaatgtttt gagctgatca attgaccatc
    24721 gcccgaaaca gaaattgacc cggcagcttg catcacgtcc tgtatttgct tctttctgaa
    24781 gaattgtttt ttcgcattcc ggcagttgtc gatacaatat ttctgatgat caccaactga
    24841 tgccgttgca ggggacttgc ggtaatcagc tttctgattg aaacaatgaa accgtcacag
    24901 gcacgcgtac accgctttga taagacgctt tgcccgggga tcgaccagaa taaacgtatc
    24961 cattttgttc atcacatagc cgaatcccag atgcgcatcc ggatcggcaa aggcaaggga
    25021 gccgcccgcg ccgggatggc cgaatgtctt tttgccgggg cccatgttgc ccgaggggtt
    25081 atcctgattc agcatgaagc ccaggctgaa tcgcgttttc aatttcaaaa cggcatcgtc
    25141 gccgccggag gtctcctcgg agcaccggtc tatttcaccg ggtgacagga cctgtatgtt
    25201 gcccgctttg ccgccgttgg caagaatacc gtatagcttt gccagggccc gggccgcggc
    25261 ctggccgttt gcggaaggaa tctcggcgct tcgccaggaa cggctgttga cgcccgtggc
    25321 gatactgaag ggattgccca gggccatgga tgttgcgcca aaaggattca ggacaatcgc
    25381 cctggttaat ttcagacagt ccgggtggat cgtggggatc cgcaccatga ccatgtttgc
    25441 cgaccggtga tgctcggctt catccagacc gatatgaata tcgaggtcca gcggaccggt
    25501 aatttccttt tttaaaaatt gtcccacgga catgccggta atatttcgaa tgacctgccc
    25561 caccagccag ccgaaagtaa tggcgtggta gccgagcttg ccaggtttcc accagggaga
    25621 ctggttctcc agagcgttga ccatggcatc ccagtcgtac agcgcctttc ccggttggcg
    25681 tttttttata gcgaccatgc cggcggtatg gttcagcagc atgcgaaccg taatggcttc
    25741 ttttccgttt ttctcaaatc ccggccagta cgccgacacc ggcttatccg gatccagccg
    25801 tccctgctcg atcagccggt gggcgcaaat ggcggtaacg cccttggtcg tcgaatagat
    25861 gttgaccagc gtatcttttt gccatggccg cctcttccag aaatccgcat aaccggccca
    25921 gatgtcaaca accgttttgc cttcgatgac aattgaaacg gcagctcccc gctccttgcc
    25981 ttttttaaaa ttgccaataa acgcctgctt caccttatca aaagccggat cacaaaaacc
    26041 attgacactt atttcagcca tattatctcc cggtattaca ttcgatcatt cttcgtctga
    26101 ttcgtttttg cagaacaaat caaccgcata agtgtgttgc cgtgcactga cctgatgcac
    26161 gccctttttc ggagctacac gcttttgcaa gctacatcaa tgcctgattg cctgtcaacg
    26221 actatgatgg cctgtaacga tactcctcct gacgataaaa cccttgcatc attttgcaca
    26281 ggcaacgctt ttgctgttgc aacaacatag gcaaaagaaa catcacgtcc catcctcacc
    26341 ggaccatgtc tgacaagtcc agtcaggctt cgggcatttc cacccgttgc gcccaattta
    26401 tttacggcgg acgtcggtag cggcattgca ttatgtattt gatatattta acgtattgtc
    26461 atttttggcc tatgtgaaaa aaggcataag tcaatattga cagaattgac aacccgattg
    26521 cttgtcgtta tgaaatcgat gttaaaaaaa atataactct tggtcagtaa gagttcagat
    26581 aatttaagat tggagaaaga catggagaaa aaaataccta tcagaattga cggaaaagat
    26641 gttgaagcga ctgccggtca gacaatttta gaatgcgcac gggataacgg catttttatt
    26701 ccgacccttt gtcattatac caaaacaacc aatgtcggtg cctgccgcgt atgcgtggtc
    26761 gaagtggaag gcgcccgatc tcttgccgca tcctgctgta tgccggtgac gcccaatatg
    26821 gttgtccgca ccgatacgga agccgttcgc aaggcccaga aaatgatcat cgaactgctc
    26881 tggtcgtccg gagatcataa ctgcctcacc tgcgagcaga acggtaattg cgaattgcag
    26941 aatctggttt actggctgca gattgacaag ccgcgctttg aaattcaagt gccgggttat
    27001 gcgaaggatg acagcaatac catgattgcg cgcgacatga acaaatgcat tctctgcgga
    27061 cgctgcgtgc gcgcctgcaa tgaaatccag gtcaacgaag ttctggattt tgccaaccgc
    27121 agttataagg ccaaggtcgg ccccgcattc gaccaggatt acattgattc aacctgcgtc
    27181 ttctgcggcg agtgcgtgca ggcctgtccc acaggcgctc tcacattcaa acaggcgaaa
    27241 ttcgccggcc gtccgtggga acttgcaaaa gtcaaaacca cctgcaccta ttgcggcgtt
    27301 ggttgtcaga tggatctcta caccaaggat aataaaatcg tcaaggtcat gggcaaccgc
    27361 gaatacggcc agcccaatga cggcagcctg tgcgtcaagg gacgtttcgg catggatttt
    27421 ctttcccatc ccgatcgcct gaaaacaccg ctcatccgtt ataaaaagaa tgaaaacttc
    27481 aaagaagcga cctgggatga agcctttacc ttcatcgcgg acaaactttc caggatcaaa
    27541 aaagaacacg gcgccgacag cattgcggga ctcgcctcgg cgcgctgcac caatgaagaa
    27601 aattacctgt tccagaaatt cctgcgggcg ggaatcggca ccaacaatgt tgatcactgc
    27661 gcccgtctct gacactctgt cacggtggcc ggtctggccg ccacattcgg cagcggtgca
    27721 atgacaaact ctatagacga aattgaattc acagacctga ttctggcaac gggcaccaac
    27781 accaccgaaa atcatccggt gatcggcgcc aaggtcaaac gggcaaccaa gcgcggcgcg
    27841 aaactgattg tgattgatcc ccgcgaaatc gatctggtca aatacgccga tatctggctg
    27901 cgccagaatc ccggcaccga tgttgccgtt atcaacggaa tgatgaacgt catcatctcc
    27961 gaaggtcttt atgccaagga atacgtgaaa gacagaaccg aaggattcga cgcgctcaag
    28021 aagatggtcg aaaaatacac accggaaaag gtcgaaaaga tttccggcgt gcccgcggaa
    28081 gatttgaaga aagccgcccg gatgtatgcc aaggcagacc gtgccagcat cctttacgcc
    28141 atgggcatta cccagcacat taccggaacc gacaacgtca agagctgtgc taatctttcc
    28201 atgttgtgcg gcaatgtcgg tattgaaggc ggcggcgtga atccgctcag aggtcagaac
    28261 aatgttcagg gcgcctgcga catgggagca cttcccaatg tttatcccgc ctaccagcag
    28321 gtggccaatg aagatgtccg taaaaagttt gaaggcttct ggaacgccaa gctttccccc
    28381 aaaccgggcc tgaccatcat ggaaatcatg gcagccgccg gcaaaggcga tatcaaagcg
    28441 atttacatca tgggtgaaaa ccccatgctt tcagaccccg atttgcacca cgtgaaaaaa
    28501 gaacttcaaa aactggattt gatgattgtt caggatctgt tcctgaccga aaccgccgaa
    28561 ctggccgatg tggttttgcc ggtggccagc tttgccgaaa aggatggaac gttttccaat
    28621 accgaacgcc gcgtacagcg cgtgcgcaaa gcgctcgacg cgccgggtga agccaaagcc
    28681 gactgggaag tcatctgcgg cattgccaac aagatgggtt acgagatgaa ttacgcttcc
    28741 gccagggaaa tttttgaaga agtggcgaaa gtcaccccca gctatgcggg catctcttac
    28801 gagcgcctgg aaaagggcgg cattcagtgg ccctgtccca cgccggaaca tcaggggacc
    28861 aaattcctgc acaaggataa gttctcccgc ggtctgggat tgtttaccgc cattgaatac
    28921 attccgcccg atgaattgcc ggacaaacaa tatccgttcc tgctttccac cggacgcgtc
    28981 ctgtatcatt accacaccgg taccatgacc acgcgtacaa aaggtctgac cgagcgttat
    29041 cccgaaagcc tggtggaaat caacccggcc gatgccgaaa aaatgggtat tgccgaaggc
    29101 cagaaagtca aggtgacctc aagacgcggt acggttgaag ccaaggcaac cattaccaaa
    29161 aagagtgctc ccggttcgat cttcatgaac ttccatttcg cggacgcatc cgtcaacctg
    29221 ttgaccaatc cggctctgga cccgatcggc aaaattccgg aatacaaggt ttgcgcggta
    29281 aaagtggaag ccgcgtagct tttaaaatat taatagaaac aaagaaagct atgcctctga
    29341 aaaggggtat agctttcttt ctctaaagtg tcattcccgc gctggcgggc catggtgccc
    29401 gtcagggtaa atccggtttt tttattaatt aagtgttatg tcccttaaat gcccaatgcc
    29461 ggcagacagg ctaaggtttc atcagtacca tgccggttaa agttccggtt aagaggctgc
    29521 cattaagccc gatttccgtg gcggcatagg cgctgttatt ggccggtgta atgccgtatg
    29581 gatagctgaa gacagatttt cctgtcgtcc aatccaaggc ttcaaatgtc caaaacccga
    29641 atctttttcc gacgccataa aacaagttgg tcgtctgact catggtggga attccattgg
    29701 ggatgcttac cgatttgttt acccaggctg tctttaatgt tcgtgtccat ggatcccata
    29761 cgaatttttc aacaccataa ggagcgattg gcgcaaatcc ggacatcaga acgctgatga
    29821 cttgattgtc cagtgacgct ttcaactggt tattgacaac cactgctcca taacccctga
    29881 cgcatacaga ctgttcagac aatgtttctg tcgcgccggg gttaccgaaa gtcaccggca
    29941 cctgtgcggc aatgcgccgg ctttttgttc cggggatctg ctgccagtcg gcgggaatct
    30001 cgtcgcgcca gaaaagaaca aggtgcatga gattctgacc atctgtaatc accacaaatt
    30061 tatcctgatt accggttccc attagcgtcg gtgtcgagcc tgaacctgct ccgagccgca
    30121 cgcccgaagc ttctcccgtt tcataaccgg cgctccatcc tcccttgtca ggatctatcg
    30181 tgagttcact gccagtccac tgcactcggt acatattttt atcggtaacg acataaatgc
    30241 ctccatgctc atcacaggct atggaattgg aaatctgttc gtcagcgggg aagcgatagt
    30301 aatatgcttc gtcaaaaaaa cgggaaacaa cccctatcaa tccatgactg gtggcaagag
    30361 tcagcatgcc gtcccaggtc atgcttagtc ccacgatttt ttccgccgga tcaacaagca
    30421 ggtttgcggg aatttcatac acgccctgaa taatgatatc cgaatagcga ttaccggcaa
    30481 tcgcatcgcc aaacgcaaat atttttgtaa accggggcat atagaatgta ttatccttgt
    30541 ccaccagagt ataagcgccg gaaagcattt caccgggtgt aaatatagat gaaatggtca
    30601 aattttcttt tttcatagtt ttgatgtaat ttagcgattc atccgccttg aacacatccg
    30661 ttgatgaact gccccaaatc acgcgtttac catccggata ttttccagag atggcaagcg
    30721 taatcaaacc gggcatcccg acatgaaaag atgatcttgt tgttaaattg attgatttag
    30781 gtcccggata cggagaggat gcctgaccgt aaggatttcg atgactcatc ggccagggag
    30841 aatcagcgat aaacggattt cggggcggtt cattcgtaaa gtttggcggc ggtatgaccg
    30901 gccaggcaaa aatctcaacg gaaaacactg tcagaacaaa caaaatcaac attgtcatca
    30961 tcggaaacag tcttaatcct tttctcataa atagcctcca gttcagcgat ttcttttaat
    31021 agtgtacggc acgtttgata ttactttaca ttgacaacga cattgagttt catctacgct
    31081 tgtgttattg accacttggt taatttcgtt ttatgctact tgatacggat atcatttaca
    31141 attgccggag aagctcaaaa tattgtcatt ttggatcaac ttaatggcga agaataatac
    31201 tgattgaatc atgagaattt ctctgaaatc aaagcctgta atcgcgccct atcgatctac
    31261 gcataacttg cgcgttatca tccaatgggc agaagagcat ggaatagata cggcgtcgat
    31321 tctctccagt gaaggactgg agaaagcgga ttttgacaac ccggatagat tgataacatt
    31381 tgaacagcaa tttgagatct cgcaaaagtt attcgacctt atttcctgcc cggctttagg
    31441 actgcgcatt ggagaacgca ttaatatcgg ccagttgggg aaatggataa tggctatcaa
    31501 tagctgtgcc acaacccttg acgcctttaa aatggctttt gagcttgccg atctcttatc
    31561 atttcccttc caatttatcc ttgatgttca ggacgatatt gccaaattca gttttattga
    31621 tctcatcgat ctgggtcgca gcagccgctt ccgttacgaa atgcatatgg taggcggcta
    31681 taatatctgc tgtgaaatta tgcgggagcg agtgcaactt atagaaatcc gttttgccta
    31741 ttccaagccg gaatatgcag aggaatataa accaatattt aattgtccga ttctctttga
    31801 tgccgaagaa actcaaattc ggttcgacag cagttacctg accagaccat tgccatgcgc
    31861 caatacgctg actaaaaaga tttatgaaaa agattgccgt caaacgcttg agcagcttat
    31921 catgcttaca acaacaacgg agttggctaa atcggcgctt cagtttaaag acaaagaact
    31981 gctcacgagg tcacagcttg cccggtgttt gaacatgagc gatcaaacgc taaagcgccg
    32041 gttagcggcc gagggtgtga gttataaatc actcgtgaaa gacatccgtc agaaaaaggc
    32101 ttgtgatctt ctgcaatcaa ccgaacttac catggaagaa attgcaaata aggtaggtta
    32161 tcgtgatgtg gccaattttt atcgcgcctt caaatcgtgg acaggcctga caccgcgtag
    32221 ttgccgtggg aaaaatgaaa agcggtaatc acccaatgga tgaacttttc attgccgaag
    32281 caatcatccg gcaggtgctc cggagaatgc cggatgcgcc ggacaggttg ctgattgctc
    32341 cgaaatattc tggttcaatc gaaggacgac gattgaacca gcgcaaaaac cgctaatatg
    32401 agtcaagagc agacttgacc cctttgcccc tgttgctttt taaccagtaa cttggaattt
    32461 taacggcgtt cctcttactc ctaaacgatc aaactatctg ctgtgtttct tgatcttcga
    32521 atttgcggaa tatttctgcc atcagtttga ccgttatact cgcaggcttg agaaatgcga
    32581 ttgtcgcatg aaccgggcag ttcctgacac aacagtaaca ataaatgcaa tttcccccgt
    32641 gcatggtggc tttaccgagc tttatgttat gcgtggggca atttttaacg cacacagcac
    32701 agcggttaca taatgattga tttactgtta tgcccggcat ggcccataca aaatttctca
    32761 gcttaacaat gtggtcatcc ataaagttta gaataaaagg cttatcattg aaatcagaag
    32821 ggctgtatct tgtacagatt ttatttttca atcggacaga tatttctttc ccgaacgtct
    32881 tgatagcgtt caaggctttc ttatccggat gccctgaata aaaaggtttt tcttcctgaa
    32941 aggtaaggca gtgttctgtt aagactctga tgccgccgat caaattatat ttcttcctgt
    33001 gcagaatttt agcaagatca tataaaacaa caccactgga taccccgcca aaagaggaaa
    33061 gcaaaacgac tgactgatca ttcgatgcat cgggcatgtt attgataaaa tcagtaaaca
    33121 cggtaggtgc atgatgaaaa tatgtcggcg agcccaaaac gatcaactgg tagtccgcta
    33181 tttttgccat aatatcgtta ttatcaaatt cactcagatt atatatatca gagctcacgc
    33241 tgttttcgtt tagcgacact gccaatgtat caatagcttt tttcgttgtt ccatgaggag
    33301 aaagataaat gattaaggcg gataatttat cttttgattt attcattttg tgaccattgt
    33361 cattgtgttt ttagttttat gttccccgaa tatgacagta acttacaatt ttaagggcgt
    33421 cccgctagcc atttattttc atttcaataa attagaattg ggaataatcc atatacggcg
    33481 cgttcaggga atgtgcccca caattaagcc gctttcagag gatggatttt ctttatttca
    33541 gcttgatgcg tctgctgcgc ctcccataga tcatgagcgc gctgagcgtt taaccaaaac
    33601 tcagccgtat tgccgaacaa acggccaaga cgtaacgcca tgatcggtgt caaagcgcgg
    33661 ctctctctta ataattcatt aaccgtctga cgcgatacac cgagtgcttt ggccagagtg
    33721 gcaacattaa gattataatc aggcataaaa tcttccttaa gaatttcacc cggatgtgtc
    33781 ggctttactt ttcttttccc atcatatttt atgctcatat tcttacctct tttaatgata
    33841 atcggttatt tcaacatcgt atgcatcacc gttaataaac cggaaacata ttctccgctg
    33901 gtcattcacg gatattgcat attgaccttc tctgttatcc ttcagcatgt gcagatgatt
    33961 gctcggcggc acttttaaat cttcaatgca cgttgccaaa tctatatatt caagacgcct
    34021 gatagctctt ttaagaatat caggctgaaa cttctttgat ttaccggtaa taaacaaatc
    34081 acgcgtatgt ttgtcggcaa atgtttttat catgtgtatt gtgtaacgtg actcgtgaca
    34141 tttgtcaagg gcaaagtttt gattctaatg atgtcctttt ctgccattgt ccaactcgga
    34201 tgcatccgtc aacctactga ctaacccggc tctggacccg accggaaaaa ttccggaata
    34261 caaggtctgc gcggtaaaag tggaagatgc gtaaatttta accaattcaa aaacccaaaa
    34321 agacatgctt cttttcagag gcatgtcttt ttatttaagg tgtcattccc gcaggggtct
    34381 ggtgggaatc cactttttct ttccaacatg agccaagcaa agacttgacc cctttgccct
    34441 ttttacaatt ttaagggcgt cccccttttc cgcttttccc cctctccgtt tcacttcggg
    34501 gatagtttcc aatagccgaa ggcttttgga tagttcctcc aacgttttgc gcgtcgtttt
    34561 tctcgcgctc aacgggtgtc actacgactt gcgcttcaaa tcaccatagg tgataattca
    34621 acctacaagt ttcaggacgc tacgcatcta aaacttgggt ttgattacat cgcaaagctt
    34681 tttttcagca agtctcatta aatagtgaat aggcttctta tcgtcagcgt gttgtagcga
    34741 caaccttctt gactcacaag aaatcgttcc ttatacttca ctcccaatag atgaaaggaa
    34801 gtttttagaa atgaaattaa ctgaaacaca gatgattgaa attgcacagc acgcgtttac
    34861 gcgcatggat ggggcctggt ttttggcgct ggcgaagaag ttcggtgttc aaaccgcttg
    34921 ggaaatggat gtggaagcct ggaaacactt tgcttatgtt ttcggcaaga acctccggaa
    34981 gaactatctc cccaaccccg tctggccgga aagtattttt gatgctttgg atattttcag
    35041 cagggtttta aagatcgagg gacggaaagt ttgcatcgaa gatggtaaaa ttatcattcg
    35101 cattacggat tgcgaaacac agaaagccat tgccaaggcc ggcgttgccg attgcggtat
    35161 tgtcaccgtc cagacctatc aggggctcat cggcggtctg tttgataata aaattttcgt
    35221 ggatgttgct cacaccaaaa atttaaatcg cggtgatgat tgctgcgaag ttgttgtcac
    35281 ccggaaatcg ccgtaaatgt ccatataaat atccaccgat aaacgggtga atagaaaata
    35341 gttcaatgtt caacgttaaa caaattgtga atcgtgaaac gtgaagggtg aagagtgtcc
    35401 cctccacttc gcttcgggga tagtctccaa tagcatagct attggatagt tcaactaacc
    35461 aattccgatg catttcactt tggtgattgg agtctcctta aatgtgttga atgcatccga
    35521 ccatcagtgg tatcaatagc aaaagtgttt catcgcctgg gcgtagggag tttatgaaaa
    35581 tttattttgc cggagcgatt cgcgccggaa ggcaggatgc cgaaatctac aaagcaatga
    35641 ttgaggtttt aatgtcattt ggcgacgtcc tgaccgaaca cgtcggcaat cccgctctgt
    35701 cggaaaaagg caatgacgga ccgcatgacc ggtttattca tgaccgcgac atggcctggc
    35761 tggaatcctg cgattgcgtg gtggccgagg tgaccaaacc ttctctgggt gtgggctatg
    35821 aactgggatg ggccacggca atgaaaaggc ctgttttgtg cctgtatcga cgccaatcga
    35881 agaaagtttt gtccgccatg attgcaggca gtcccggcat ccggacggtt gcctattcct
    35941 ccatggacga agcgagaagt attgtggaga aatttatcaa aaaggccgtt caaaaaatat
    36001 caacttcttc cgggagagaa tagatggtca catcactgga attgctggcg ccggcaggcg
    36061 atgccgctat cggaatagca gccatcgatc atggcgcgga tgccgtgtat atcggcgcgc
    36121 cgaaattcag cgcccgggcc gcagccggtg tgcagctcga cgatatcgcc cgtctgattg
    36181 aacacgccca cttctatcgg gcgaaagttt atgtggcgct caataccatt cttaccgatt
    36241 cggaactggc cgagtcgctg gatctgatca gggccatcga tgcgctgggc gccgacgggc
    36301 tgattattca ggatgtcggt ttgctggagc tggacttgcc gcccattccg ctgattgcca
    36361 gcactcagat gcacaacagt acgccggaga aggtccggtt tctggaggcc gtgggatttc
    36421 agcgggttat tctggcccgg gaactttctc tttccgagat tgccgggatt cgccgtcagg
    36481 ccccgaagat agaactggaa acgtttgtgc atggcgcgtt atgcgtttcc tacagcggac
    36541 aatgttatat gagtcatgcc gttgcggggc gcagcggcaa tcgcggcgtt tgcgcgcagc
    36601 cctgccggtc ccattacacg ctgatcgacg gtgacggcaa caccataatg aaaaataaat
    36661 atctcctttc gctcaaggat ttgaacctga tgagccgggt gccggatttg attgccgcag
    36721 gctcgacctg ttttaaaatc gaggggcgat acaaggggat ggactatgtc aaaaacgtca
    36781 cggccgctta ccggcaggtg atcgatcgat tcatcaacga gcacgccggt taccggcgta
    36841 gcagttccgg cgcaagcgag cttgcattca cgcccgatgt cggcagaacg ttcaaccgcg
    36901 gttacagcca gcactttgtc ggcgggaaaa gagaaaagat cgcttcgctc gatacgcaaa
    36961 aatccatcgg ccagtatctg gggaaaatca ccgcggcggg aaaagattat tttcaaatcg
    37021 accgtgatga tctgcaaaac ggggacggac tttgcttttt caccaggcaa aacaatctgg
    37081 ctggctccag agtgaaccgg gtagagaagg ggaaggttta tcccaacagc atgaaaggcc
    37141 tggaggccgg actgtcgctt tatcgcaatc tcgacaccgc gtttgccaaa gcgttaaaga
    37201 aaacatcggc caaacgattg attgcggtgg agatggattt cagccaggcc gatacgttga
    37261 tccggctggc ggcaaaagat gaagacggca atcaggtgga agccgtaaag gatgttttgt
    37321 ttgaaccgtc gcgcaatccg gccggagcaa ggggccaaat cgaaaaacaa ctgaccagcg
    37381 ccggcaacac gccgtttagg atatcgcgcc tgaccattga accccgcgcg ccgggctatc
    37441 tttccgcaag cgtcttgaac ggcatccggc gcgatgttct ggaaaatctg gcgcgcgtga
    37501 gacgtgaaac ctatgttcgc ggggaccggc cgttttgtcc caatgacgtt gcttatccgg
    37561 aaaaacacct cgattttcac gccaatgttt tcaatgcggc ggccagacgg ttctatgaac
    37621 ggcacggcgc aaccgttacc gaaccggcct ttgaaacgct ttccgatttt gccggtaaaa
    37681 ccctcatgac cacgcgctac tgtatccggt atcagttgga tctgtgcccc cggctgcagc
    37741 aaccggagtc ctccgtcaga gaaccgttgt atcttcggga tgctcatcat acctatcgtc
    37801 ttgaattcga ctgcaagcac tgccggatgt cggtaattct ggaaaactga cggaagcgcg
    37861 gaaaccggat taaggcgcgc gggtatttct atccgttaag cggggatcaa ttacagagta
    37921 ttaaaaatac tgcaccggcg cggatggtca ggaatcttta agcgcgtact gaaaatacat
    37981 atctttatcc cctgcggcaa ggctcctgat cctttttgca gacgatttct tcccgaacat
    38041 ttttatggcc tgctggaagt aacccatcac gaggtattca aaatacgtat atttgatttc
    38101 aagaccggta atcttgatat gagcaacatt gttttcaaac tttgcaacgg cgacgccgct
    38161 gtcaaaataa gaagaccaga ccttgggcag caccgtctcg ataaattgtt tcatgtcatt
    38221 tgtcatctgc ataaacgacc tgtagggtcc cgtcggcgac agaaccgctt tggcgcccgc
    38281 cttgccgaaa tat
//
LOCUS       DAOSME010000019        37314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1488_length_37314_cov_24.642878, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000019 DAOSME010000000
VERSION     DAOSME010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 37314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37314
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1488_length_37314_cov_24.642878"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLG98_04515"
     CDS             complement(<1..197)
                     /locus_tag="PLG98_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32548.1"
                     /translation="MNHYDLFKSWHQLMTQTTDMACNKKIKSEAPVEPLGALASWVDL
                     GTRLSHLTWARFSEDAWEYSI"
     gene            565..915
                     /locus_tag="PLG98_04520"
     CDS             565..915
                     /locus_tag="PLG98_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32549.1"
                     /translation="MNNGSTKMTDIKIDTNNLYKEEAFTDLTFATIRRLNPVNLDGSP
                     DASREPIFTGMTQLMSPNGPIPVQCLIEGAKTLAEAAEKLPVAIEKTVQAMIAEAKEM
                     EREEASKIVIPGQS"
     gene            complement(947..1129)
                     /locus_tag="PLG98_04525"
     CDS             complement(947..1129)
                     /locus_tag="PLG98_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32550.1"
                     /translation="MTENKQPLPENTWKCSNCGNTVVQITPPETCPVCKQKCEFLNVT
                     CYQPDCGFTGMDKRLK"
     gene            1276..1680
                     /gene="queF"
                     /locus_tag="PLG98_04530"
     CDS             1276..1680
                     /gene="queF"
                     /locus_tag="PLG98_04530"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOE32551.1"
                     /translation="MPKEKKKNDFAGLTLLGKAAKPVRKLETFPNHNEKRAYVVTLQT
                     EEFTCVCPKTGQPDFAKIKIQYIPDKKIVESKSLKLYLWSFRNEGVFHEHVTNIILDD
                     LVAALKPRWCKVSAEFAVRGGIGITVDAEYKK"
     gene            1704..4904
                     /gene="carB"
                     /locus_tag="PLG98_04535"
     CDS             1704..4904
                     /gene="carB"
                     /locus_tag="PLG98_04535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOE32552.1"
                     /translation="MGKRTDIKKVLIIGSGPIVIGQACEFDYSGTQACKALRNLGYEI
                     VLVNSNPATIMTDPGMADVTYIEPLNIQALTEIIENEKPDAILPNLGGQTGLNLTSEL
                     YKKGVLEKHNVKVIGVQVDAIERGEDRIAFKETMNKLGIDMPKSEPATSVEEAEKIAT
                     KMGYPVVVRPAYTMGGTGGGIVYNLEELRLIASRGISASLIGQILIEESVIGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGVHTGDSYCTAPMLTITPELQKRLQDYSYKIVEAIQ
                     VIGGTNVQFAHDPKTDRVVVIEINPRTSRSSALASKATGFPIALISSILASGMTLDEI
                     PYWRDGSLEKYTPSGDYVVVKFARWDFEKFPGSEDKLGTQMRAVGEVMSIGKNYKEAL
                     QKAIRSLEKKRYGLGFVKDFNAKSLDELMEMLSEPSSERQFIMYEALRKGASVETLSA
                     KTHIKKYFIEQMKDLVGLEERILQYKKKKMPDELLTQAKKDGFADKYLAQILGKEEEA
                     IRKQRQALGVTEAWEAVPVSGVENAAYYFSTYNAPDTVPVTGNRKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALRDEGIESIMVNCNPETVSTDYDTSNKLYFEPLTVEDVLSIYEK
                     EKPEGVIVQFGGQTPLNIAAELAKAGVKIIGTSPETIDLAEDRDRFRMMMDKLGIPMP
                     KSGMASNLKQALKVAGDIGYPLMVRPSYVLGGRGMEIVHDEEMLKRYVNAAVGVTPER
                     PILIDKFLENAIEAEADAISDGTDAFVPAVMEHIELAGVHSGDSACVIPPVSIPLRHI
                     ETINDYTKKIAVEFGVIGLMNIQYAIANDVVYILEANPRASRTVPLVSKVCNVSMARL
                     ATQIMLGKKLKDLNLKNKTFRHFGVKEAVFPFNMYPEVDPVLGPEMRSTGEVLGLADS
                     FGLAYYKAQEATQQNLPSEGTVLITVEEKDKGGVLEAARAFKKIGFQILASEGTHRFL
                     KENGIEAEKILKMHEGRPNIVDAIKNGEIQLVINTPAGRLSKYDDSYIRKAAIKYKIP
                     YITTIAAAVAAVKGIAALKQGHGRAKSLQSYHAEIQ"
     gene            5156..8668
                     /locus_tag="PLG98_04540"
     CDS             5156..8668
                     /locus_tag="PLG98_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32553.1"
                     /translation="MESKDGKEDKNVNALLSLPDSTKTLGKKSKSREVNPSGLKRKVS
                     QDTRRMPQKDKPAISGASPKTAGHKNGKESFCGPEEKYALAFKYSPIGICISSLQDGR
                     YVEVNEVYSNFLGYRREELIGRTSSEINLWVDMAERDALVRELLEKGTVTDFQLRFRD
                     RNGNVHWGNVAAALVTIGQELYVLTQTQDVTDKKKIKEQFEKSEAKYRLLAENLTDVI
                     FTIDMNFNYTYASPSTYHLLGYTAEELMTMKVENLVDPETLAWFMEMLAEEMEIEKRP
                     DRDLKRSRVLEYQHIRKDGSTIWVENKITFLRDQDNNAIGIIGTVRDIAERKRAEEQV
                     CKEIDFNRSVLDSLTVPFFMFDTASMTFFRWNKTFSAVSGYSDEELGQMRPSALISEH
                     QKDFLLAKFEEFQRTGHIAFEMPVLSKNGSVTPYLLSGNLLHHDGKPFVVGMGIDITE
                     RKKAEESLRRSEEKYRTILENMEEGYFEVDLSGNFTFFNDATCRHFGYSREEMTGMKN
                     RQYMDEKNSKALYIVFNRIYETDQPVTNLEWQIIRKDGAKRDIEGFVFLLKDVAGTPI
                     GFRGIVRDITERKQVQEKLHRERQLFKSVTEQSSDIIIIADTRGRITYENPAVEKILG
                     YRVEERLGASGYDLVHPDDAQIVADAAGKLFHNIQAPVQKGEIRLHHKNGSWRTFEVT
                     ASNLVKDHVVEAAIVNMHDITARKRSEELIRQSEAKYRLLADNMKDYVWLMDLNLKIN
                     YISPSVERLMGYSLEDFKKFSLDKFLTVQSFQAAMDFFAIEMPKALGAPPDYVLERSL
                     ELEFCCKNGQTVWGECNFSLIRDDKGKPLSLLGVSRNINERKQMEDALRKSEENFRHS
                     LDDSPLGVRISTVEGKTLYANRAILDMYGYDSKEELQNTPLQERYTPETFEAFKERKK
                     KRLKGQFGPSEYEISIIRKDGEIRHLHVFRKEIFWNGQKQSQVIYEDITLRRRAENRL
                     NETLESLRQSIKITIQVLGTASEAKDPYMAGHQRRVADLARAIATEMKLPQDKIEAIR
                     MASAIHDIGKISVPAEILCKPAILSDIEFSFVKNHPRYSYDIMKEVESPWPLADIVYQ
                     HHERMDGSGYPLGLKNDEILIESRILAVADVVEAMISYRPYRPALALEMALAEIEDNA
                     GTLYDAEVVKACLRLFRDKNYQMS"
     gene            8780..9478
                     /locus_tag="PLG98_04545"
     CDS             8780..9478
                     /locus_tag="PLG98_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOE32554.1"
                     /translation="MKKLTAIILFVLCLIPVVIFAKNASVEDQFKKSFPKNKFESIVP
                     TSIKGIYEVYSGNQLYYYMPKDEIVLYGTLVSKSGVNITRDSLLKKMAQKMEKLPLEN
                     ALKIGNGKKIIVEFMDPHCYHCRQAYQFFSQRKDVTMHVFFFPLSKESADQIKHIYCS
                     KDVLKTYDDVMKGKLDNNASLNVCADAKIDEKIKTHVQLASQVGLRGTPLFYIKGQVV
                     DGFDVALIEKLLKD"
     gene            complement(9517..11412)
                     /gene="metG"
                     /locus_tag="PLG98_04550"
     CDS             complement(9517..11412)
                     /gene="metG"
                     /locus_tag="PLG98_04550"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOE32555.1"
                     /translation="MPKSFYITTPIYYVNASPHIGHAYTTIVADVLARYHRMIGDKTF
                     FVTGTDEHGDKIAEAAQKAGLTPKQYADNISAQFRALCPQLAISNDYFIRTTDANHIE
                     TVRYILQKVYDAGDIYFGSYGGYYCFGCERFLTEKEIVDGKCPEHGREPQYIEEKNYF
                     FKMSKYQDWLIDHIKKNPEFIRPERYRNEALAFLRDPLEDLCISRPKTRLEWGITLPF
                     DEQYVTYVWFDALINYVTAVGYPEGEHFKTFWPVAEHLIAKDILKPHGIYWPTMLKAA
                     GIEPYQHLNVHGYWNSDQSKMSKSLGNIVRPLDLKDKYGLDAFRYFLLRDMVFGLDSN
                     FSEDAFVGRLNSDLANDLGNLVSRAITMAIKYCGGRVPAVAGDEKNPALREAALKAVL
                     DVEASFRDMSLHKALIAVWDFINITNKYIVENEPWSLAKDPANGKKLTAVMYDLLSAL
                     HAIAVMLSPFMPQTAEKILRQIGCEKTEGLKLDDIKTDNALKTGQVLTRSDALFPRIV
                     QQKEKPEDQKAAMTDLKPEIDYETFSKVDLRAATILAAHAVPKSNKLVKLTVDIGEER
                     TVVAGIGKDYKPEDLIGKQIVIVANLKPAKLMGVESRGMLLATDTPEGLTLLTFDRAA
                     KTGGKIR"
     gene            complement(11430..12188)
                     /gene="ricT"
                     /locus_tag="PLG98_04555"
     CDS             complement(11430..12188)
                     /gene="ricT"
                     /locus_tag="PLG98_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOE32556.1"
                     /translation="MLVNIIGVKFKKEGRIYNFDAADLILHRDEQVLVETDSGLALGT
                     VVTDVKRCEPSAVPANLKKIIRKVTDDDLKIRKENDALEQEAWKFCLERIRKRELSMK
                     LVNVDCLFEKNKIIFYFTADSRVDFRELVKDLVQKFKTRIELKQIGARQETRITKGLG
                     ICGRMVCCAGILQNLERVTVKMAKEQSMSLNPEKISGLCGRLMCCLAFEYDGYAGKKK
                     AAKAAKTSGSATEAQEEKQTLDLEKMDDIDHLQS"
     gene            complement(12206..13186)
                     /gene="holB"
                     /locus_tag="PLG98_04560"
     CDS             complement(12206..13186)
                     /gene="holB"
                     /locus_tag="PLG98_04560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOE32557.1"
                     /translation="MSLGNIYGHEKKIDMLRKTLARRRIAHAYLFSGPSATGKKTLAV
                     EFVKAVNCISSDDRHDACDACPSCRKIQHASHPDIFFVEPEGQFIRIASIRQIQERTT
                     CRPLEAKKRAFIIDDADRMNEESANALLKMLEEPSPANMLILITTRPHAMLQTIISRC
                     QHIHFNPLAVETVARFLVSEKNMEHSRAMLLAALSGGTIGNALELNQEEVVAYRTELF
                     NTLALTDRKEPFSLIHFASFLGQKKKEIRQSLNILNTFFRDVLVFKEVGQKGMLINQD
                     NTPLIAACAARLTGKQILQNIAQIEKAGDIIEQNVNKSLTLETMAFKLNY"
     gene            complement(13220..13870)
                     /gene="tmk"
                     /locus_tag="PLG98_04565"
     CDS             complement(13220..13870)
                     /gene="tmk"
                     /locus_tag="PLG98_04565"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOE32558.1"
                     /translation="MIKAKKFITFEGIEGSGKSTQIKLVAEYLQNQGVELIVTQEPSG
                     TDIGRQIAGILFHRRHNHLCAEAEMFLFCAARAQHVRDVILPALRQGTLVLCDRFSDA
                     TYAYQGFGRGLDQKFIGAVNDYSAMQLKPDMTLLFDLPVETGLERATARNNRLADPAA
                     TDRFERETLDFHRRIREGYLTILNNEPERFRLIDATRKIPVIQEEVRRHISSFIKV"
     gene            complement(13995..14174)
                     /locus_tag="PLG98_04570"
     CDS             complement(13995..14174)
                     /locus_tag="PLG98_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE32559.1"
                     /translation="MAYVITDECIACGSCESECPEGAISEGDDKYVIDPKLCNDCGTC
                     ADQCPVEAIVPGEGK"
     gene            complement(14286..14507)
                     /locus_tag="PLG98_04575"
     CDS             complement(14286..14507)
                     /locus_tag="PLG98_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOE32560.1"
                     /translation="MKEDVQKAIDTIRPSLQADGGDVELVDVSDDGTVKVRLTGACHG
                     CPMAQMTLKMGIEKVIKKLVPGVKEIVAV"
     gene            complement(14555..15151)
                     /locus_tag="PLG98_04580"
     CDS             complement(14555..15151)
                     /locus_tag="PLG98_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32561.1"
                     /translation="MIKKKSYVFVWIALILTIAGCGGLHFSQLAPEAIDFHPQSIAVF
                     PIEVWNHKEPDNRTVVQQIVAGSLIAKKLFTSVTDAEKLQKQMQNDELLKKTAEEYFS
                     KLQLLKFSDADLSRKIGDILQVDGILLLSVDEWKYFVEADKKMAQVSLTMELYDVTNG
                     KLMWKANHTIKKDYVLIKPGLPDMARDVANKMVYYMPH"
     gene            complement(15193..16920)
                     /gene="ispH"
                     /locus_tag="PLG98_04585"
     CDS             complement(15193..16920)
                     /gene="ispH"
                     /locus_tag="PLG98_04585"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOE32562.1"
                     /translation="MEIRLAKTAGFCMGVRRAVDIVLDIAQHEKNRRIYTYGPLIHNP
                     QTIELLKSRGIKPITHIDEIEDKENAILIIRAHGIAPAERKKIKETGIKVVNATCPKV
                     GYVQSIIKKHAAQTYTVVIVGDKEHPEVDGLLGYTDGRGIIISTEEEVNKIPCLEKVC
                     VVAQTTQNLDEYQKIVEKIKQLCPQAVVFNTICLSTEQRQAEVIDMAGEMDAMFIVGG
                     KNSANTKRLADLARKNQPSTFHIETPADMEKIDLGPYNKIGVSAGASTPNWILDRVID
                     KITEGRSRKLKNLGKLLTLWTFLVKTDIFSAIGAGCLCLVCMLLQTMTVRFSFILIAS
                     LFVYGMHVLNRLISRKPAGLVGSFREEYYIRHENIFFVTSLFSIILALVLAFQQSLAV
                     FFSLLIISLGGVLYNMQLLSPRWNFQSLKDVPGSKNIFMSIAWAVAAAFLPSIHETPF
                     PDAGLLVAFFFVFCIVFIRSAMSDILEIQSDKLIGKETFPVLFGKTRALNILKIISLI
                     LFAILLFASPSGWTSSLSFPLMFCLLYLWICFLFCDKRPVLSGAVKEGLLETSYIIAG
                     FCVLLWLIF"
     gene            complement(16910..17833)
                     /locus_tag="PLG98_04590"
     CDS             complement(16910..17833)
                     /locus_tag="PLG98_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOE32563.1"
                     /translation="MKNKHLIYVLFIIVLFSTYSELLWTASGRTSPPYYNMAMGAVFT
                     CFALLHGGTYVGRKCIISLAVLTFVISFIMEYTGVKTGLIYGEYHYGDALGIKIFDTV
                     PLLIPFSWFMFMYVSTVTVDAAFGDRFAGRSAPFLFAFFDAAAMTALNVLIDPLWVTG
                     GTWTGTTVQALPSGSVFYGIPAQNYFGWLLTTIMIFIPYRMIFFRNRNRFAARDRDFF
                     LPCLIYASIITVGSIKSWMVLANTGIIFVTIMTGGVFSLAALAGFLSCRSGRSGPAEK
                     RQRQGHPDSFQEDDQISYNNKIARKTKQYGN"
     gene            complement(17880..18632)
                     /locus_tag="PLG98_04595"
     CDS             complement(17880..18632)
                     /locus_tag="PLG98_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOE32564.1"
                     /translation="MAAEKIRLDKLLVERGIAPTLEKARALILARAVKVDGAYPDKAG
                     TLFVPHTEIHIKGEDNPYVSRGGLKLAGALKEFDMNVEGLVAFDVGASTGGFTDCLLQ
                     AGARKVYALDVAYGQLAWKLREDKRVVVIERTNIRYYDGADLDEKIDLAVIDASFISL
                     KTVIPPVLKFLEEGTRILALIKPQFEASKKDVGEGGVVRDPTIHDRVVQNISDFCISL
                     DLDVEGTCASPISGPAGNKEFFVLTRKKPSQT"
     gene            complement(18675..20597)
                     /gene="dxs"
                     /locus_tag="PLG98_04600"
     CDS             complement(18675..20597)
                     /gene="dxs"
                     /locus_tag="PLG98_04600"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOE32565.1"
                     /translation="MVKQPQTVHYSVLKDVNFPADIRGLTLAQLNTLAQEIRDLITLT
                     VASCGGHLAASLGTVELTLALHYAFNTAQDKIIWDVGHQSYAHKIITGRKEDFKNLRK
                     QNGISGFPRREESPYDVFNVGHSGTSISAAAGMTEAACLKGASYKIIAVIGDGSMTSG
                     MAFEGLNWVGDRKKNMIIVLNDNEMSISENVGAMSSYLNRVMTGHKITTLKADIKRFL
                     KSIPGVGGDMIKFAQKIEEILKTLMVPGALFEEMGFTYVGPLEGHRLDHLIKTFENIR
                     EMTGPVLVHVITKKGKGYSFAEQNPPSYHGVAPFDVQTGQVIASSSGIPTYTNVFGRT
                     LVKLARTDSRIVAITAAMREGTGLNLFSQEFPNRFYDVGIAEQHAVTFAAGLATEGFI
                     PVVAIYSTFLQRAYDQIVHDVCLQKLPIIFAVDRGGLVGEDGATHQGLLDYSYLRSIP
                     HIVVMAPKDENELQHMLKTAVDCGQPVSLRYPRGSGAGRPLDEVLTALDIGKGEVLRG
                     GTDLAVMAIGSTVLPALAAANRLAEEGRDISVINARFVKPLDEELILKTAAAVGTIIT
                     VEENTLQGGFGSAVLELLAEKSITGIRVKRLGIPDEFVRHATQAQQRRRYGIDEEGIM
                     AAINEILTSESFRRPS"
     gene            complement(20616..21518)
                     /locus_tag="PLG98_04605"
     CDS             complement(20616..21518)
                     /locus_tag="PLG98_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE32566.1"
                     /translation="MMQDDDLFLKAYLEDRQNIVEEALQRYLPEDGFPSDIYKAVHYS
                     VFNGGKRIRPILCLAAAEAVGGDLVPAIPVACALELIHSYSLIHDDLPSMDNDDFRRG
                     KPTCHKVFGENMAILAGDALLTEAFVLLSRAEKVMLSAERRLAIIQEIANAAGINGMV
                     GGQALDVISEKTGADETILQDIHRRKTGALIVAAVKSGAVMFNAGKDKIQALTEYGMH
                     VGLAFQIADDILNVEGDSRLMGKGTGSDAARNKMTYPSLMGLQQAKEKLNQHVEAAVT
                     SLADFDERAMPLIVIARYIRDRKS"
     gene            complement(21505..21744)
                     /locus_tag="PLG98_04610"
     CDS             complement(21505..21744)
                     /locus_tag="PLG98_04610"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOE32567.1"
                     /translation="MMAKEKFEDALEKLEDIVKKMEAGDMPLDETLKSFEEGIKLIRF
                     CSAKLEETERRVELLLEKENTLQVKRFQGDDHDAG"
     gene            complement(21867..23198)
                     /gene="xseA"
                     /locus_tag="PLG98_04615"
     CDS             complement(21867..23198)
                     /gene="xseA"
                     /locus_tag="PLG98_04615"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOE32568.1"
                     /translation="MEQILTVSELNNTIKSLLEESFDSLLVEGEVADLRRPQSGHTYF
                     TLKDDKSQIRAVFFRPFGRTGGRVNFELEEGLKILCRARLTLYPPRGEYQLVVESVEP
                     LGVGALQKAFEQLKAKLSAEGLFDKQYKKALPFLPERIGVITSPTGAVIRDILNVSRR
                     RFPATDILIAPVRVQGNDAANDMIQALRRFHAFGQVDVIILARGGGSLEDLASFNDER
                     LAREIFRSDIPVVSAVGHETDFTICDFAADLRAPTPSAAAELVLPDRTELIFKVNMLA
                     RRMTAGCLRHLKAEKEHLEGLQERFKDPRLAVVTQKIRIDDLKESLSAAFGHRRQILL
                     HKLNHLELRLKHQSPEQQINEKQIVLKNLRKELHSSLSCVLTALKERFQKDAAVLESL
                     SPLAVLERGYSLTRTVPEGTLIRQTRDLHEGDEVNVLLAKGNFNAKIQKIF"
     gene            23381..24706
                     /locus_tag="PLG98_04620"
     CDS             23381..24706
                     /locus_tag="PLG98_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE32569.1"
                     /translation="MFWKKIKNAVCPVKHILRVPVGLFIVALVCADAGAAQNNDRLIQ
                     AAEKGLLQDVQTALAQGADTNAKSVKKYYNIFGKEITPLIAASENGHAEIVKLLLQKG
                     ADPNFQRLPERTTALMLAAENGHADVMKILLEGKADINAINNTGITALMLASKNGHPD
                     AVGILMQGKVDVNTRNNDGVTALMLAAQYGHHDIVQRLVQHQADVKIKNNDGVTALML
                     ASKNGHAKVVERLLETDAGVNVQRASDNATALMMAAENNHREIVGLLLNKGADVNLKT
                     KFIGATALILTAAKGHADVARLLIDKGADMNAKNKDGITVLMVASLNGHRAVVQLLLD
                     KGVPVNGKSNENITALLMASERGHKDIIQILLDKGADVNAKDSDGLPPVLLAAQKGHK
                     DVVTLLLDKGADGNVRAKIKDTEYTLLTAAEINKWTDIVERLKKSGVKE"
     gene            complement(24957..26414)
                     /locus_tag="PLG98_04625"
     CDS             complement(24957..26414)
                     /locus_tag="PLG98_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32570.1"
                     /translation="MMVSSRQKKRIVLVHPRGFNWFPGKRDITDIANRMVPQGVLSIA
                     AYLKQKGHEVFVYDCLGPGAPLDLQKQAGAILAYQPQMVGFSATTSSFPDAADLARLI
                     KNHSSAIATVCGGVHVSALEGKLLNAYPAFDFLIAGEGEETIAELADEIPAADIRGLI
                     WRNQDEITVNDVRPKISDLDSLPFPAYEQLKGFPRDYHLPLFSYIHTPGATMITSRGC
                     LYQCSYCDRSVFKKGFRYNSAGYIYDHMKYLRETFGVRHINIYDDLFTANRPRIVKLC
                     EKLSRRPLGLHFNCAVRIGYTDDDLLDMLKDAGCLMVSLGIESADPAMLARHKSGINL
                     DDVRDTVKRIQKAGLRAKGLFMMGLPGETEASVKRTSDFILSLDLDDMNMAKFTPFPG
                     APLWTAIREEGTFHEDWRLMNCLNFVFVPRGIASKERLDQLYNEYVKRFYSDKKWRKK
                     FRNRIWQHRNSLLYLIRHLPSFWSAKNQFEPQKGK"
     gene            complement(26411..27400)
                     /locus_tag="PLG98_04630"
     CDS             complement(26411..27400)
                     /locus_tag="PLG98_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32571.1"
                     /translation="MTETTTKHTAPASPIPVIAASYIGPPLMPNRDALWEENELPLNA
                     DALVNEVMGQPMRRAGRFIKMVSCGGLACLQQVSPDYLKGKKTGIFLATGLGNVDEIL
                     PFITQVFKHGGGFPSPNQFANSVSNAAAFVMARICEVTGTVLTVSQEELSFESALWLA
                     QTYLEHGRIDIALVGGCDIFTPTAEEYRERVNVSCGNARRLPMGESSSWLLLGKNEEQ
                     NIGHILAVDFYDTVGAPDQRFDKQTILSDLCDARGKGRIVLPGFRIRQTDLNGWKDGG
                     WETEDYLPCCGIHSTASAFGIARTLREKTNSVFVHYNCSASGRHAFVSGSTKS"
     gene            complement(27443..28639)
                     /locus_tag="PLG98_04635"
     CDS             complement(27443..28639)
                     /locus_tag="PLG98_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOE32572.1"
                     /translation="MNSGDIYVRGWGAVTTLGWNATDAARNLIEAQAPPAKPGRSSHL
                     INRDFNVFEVSHPGNPLEKAMAMLEGAIDEAFERAGLSPDEIAESSVLIGTTGGLFIR
                     NEHEFIEAVRKNPDGETPPISCRNRGAGEVADFITKKYGIRGMALTFSIACVSSSHAL
                     VVAADLLQSRKIRRAIVIGFEPLLNMTLHGFSSLLLFDYERCRPFCATRAGMQIGEAA
                     SAVILDSGQNLSSRYTFSGGYLYNDIKSMTSAGIDGVTVSEVVRHTLDSARLSADDII
                     AIKAHGTGTKDNDLSEGRGLVRLFGEKLPPVVSLKGAIGHTLGAAGTAETALWLSCLD
                     EGIIPRSFGFSEMDPEIGFTPSQKNIDARDGAYLFTFFGFGGTCTSYLIIRNSTENSS
                     ARAGSS"
     gene            complement(28636..28947)
                     /locus_tag="PLG98_04640"
     CDS             complement(28636..28947)
                     /locus_tag="PLG98_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32573.1"
                     /translation="MDVTQKLKDLNVSSREELIYKLKELVIRACEITDVRPEDVPTDV
                     PFIGGPGPLLLDSLDAMEIAMEIRFQFGVELKNASTAAKAMQNFDTLADFIIEAPRVK
                     K"
     gene            complement(28938..30059)
                     /locus_tag="PLG98_04645"
     CDS             complement(28938..30059)
                     /locus_tag="PLG98_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE32574.1"
                     /translation="MTFRKLTALIRKEMIILLRERQTIPLLFIMPMALIFFLSLALQG
                     VYMDKLTGRQILLIIENESHQPTANLLEQRISANKMIQRVQRPPGFDNDKVFETSRAH
                     AVIFIPKGFDEGLKPVEMEFDPVLDAGYKSAVRVLVTGLTVEVVMGIDDIEGMSADFI
                     VEKTKTNRTLPGPLQNSVPGWSIFAMFFIAIPMSIGFLREKNDGTLQRIFTYPVSPAI
                     VILGKLIPYYLINLMQFALMLIVGIYIMPLIIAFPFTLGEHPWHVIPVTLVVAAASTG
                     FGVMVAALARAPEQSSALAATGAVLMAVFGGIMVPHAVMPLIMKKLAMLSPMYWAHQA
                     YLDIFSRDAGFGSIWPKLAVLTVFALVCFYIAGRKVKWM"
     gene            complement(30075..30821)
                     /locus_tag="PLG98_04650"
     CDS             complement(30075..30821)
                     /locus_tag="PLG98_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32575.1"
                     /translation="MKALDIAQVTFSYNPKEGKIKTAGAEYALRDFSMSVDSGQMHAL
                     LGPNGAGKTTLMRLITGQLRGFQGSISIYGTRNDHAQSLSSVGYAPQPISVYAGLTAM
                     ENMRVFGVMAGLSRELLQERSRAVLEETGLLPYAQKRASTYSGGMLRRLNLAVALLHA
                     PRLLLLDEPTVGVDPQSRNHIYETLKKMNASGIAIVLCTHIMDEAQRMCSKVTMLDRG
                     AVIFSGAMNEIDHLETFFLAQTGHRLRDED"
     gene            complement(30818..31855)
                     /locus_tag="PLG98_04655"
     CDS             complement(30818..31855)
                     /locus_tag="PLG98_04655"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOE32576.1"
                     /translation="MTHTELTSGGKPFVHHHAAHCETGVTAALFRDKGLEISEPMIFG
                     IGSGIFFGHLPFVKFVGLPISTYRAQPGAVFRKAAKRLDGKFVTKKYTNAKKGMDELL
                     RVLRSGQIVGLTTNIYWLSYFPDRFRFQFNGHNIVILHEIENGFRVSDPVLDKPVDCR
                     TEDLIRARFARGPLEPHGFMYYPVSVNLHPDLRGACIKGMLDTCNMMLRIPVPIFGIR
                     GIRFLAKRIIKFEKTQGFEEACRQLGMIVRMQEEVGTGGAGFRYMYAAFLQEAADLFG
                     SAELNDLSQDMTAIGDTWREFAVTAARIIKKRGKQDETFAKAGGLMLICAGREENLFK
                     NLHEIAGKLKS"
     gene            complement(32083..32496)
                     /locus_tag="PLG98_04660"
     CDS             complement(32083..32496)
                     /locus_tag="PLG98_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32577.1"
                     /translation="MKVNEVPQDNTVEYYEGVKRACYAVDEKGRYVIVSSNGWEAEEL
                     VNGLAVKELAVNLEKTRKAVLAGEKSPLAYHMERRQMIPDILGKTAGIAVFRVKRHLR
                     PEIFSRLKNPVLERYAKALAITMEELKTVPEESRE"
     gene            complement(32763..33656)
                     /locus_tag="PLG98_04665"
     CDS             complement(32763..33656)
                     /locus_tag="PLG98_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE32578.1"
                     /translation="MKKLPYKLLLFFTHRLGLWFFRIISWFIATGYFFLFPVRVANSF
                     EFYRTLFPGKGFFYHLWCAWQQYHKFTDVYIHRFIRFDENDIEYIKEGWEHLDAAVQN
                     KTGAILLMSHIGNWELAAQTLNKRGLPIMLYLGAKHKEQIERIQKEAMAKSGIKIVAT
                     SEDEKSPFALIDGINFLREGGIVSMAGDRLWGDQSHIAVNFLGHEVRLPDTPHLFALM
                     TGAPLMTFFVYQEAVGKYHIRISPGRKVTAAARADRKKAVQNSVQAYADDLAEFARQH
                     PFEWHHFEPFLEKSHSASLRE"
     gene            complement(33756..34826)
                     /locus_tag="PLG98_04670"
     CDS             complement(33756..34826)
                     /locus_tag="PLG98_04670"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOE32579.1"
                     /translation="MSVVFSGCSGSSGSDSESSPRIATTSLPSCVIASAYSQQISVTG
                     GNGTKMFSITKGSLPSGITLSSSGRIAGTTWAAPGVFQFTVTVEDAAGSSTQDLSLAV
                     VADNIAGPHPYETIRNNPSYYYVSQEAGLCAATCFYMTMKYYGDHLKNIKTGIDDDNC
                     PAEITDRIAYPTELSATSQIATYIQYMDNSFNNLAGIHLTSLARAAENLLDESGSRAL
                     YSEVMIENYSNDLTGTEDSISEQKRNIFLNRMVPFLNSGSPVLVHLWRSPVLGIPSSG
                     HYVLVIGYDDAEKAVYFMDPNDQNHKGNQCDCVAVDSSNSDICFIQKVAFDHFIEEYW
                     YKSDDPLVLNARWDGNWIGFHH"
     gene            complement(35042..35812)
                     /locus_tag="PLG98_04675"
     CDS             complement(35042..35812)
                     /locus_tag="PLG98_04675"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOE32580.1"
                     /translation="MNIIQTIILAAIEGITEFLPVSSTGHMILASSLMNIHDNAFVKS
                     FEIAIQIGAIGAIIMLYYKRFLKGIKIYIKLGIAFIPTAVIGFLAYKTIKMHLFNPLI
                     VSTALIVGGVILILVDKRVVNSKTQYMELEDISYKHAILIGLAQCVSMIPGVSRAAAT
                     IIGGVFNGLNKKQATEFSFLLAVPTMCAATGYDLIKTDAAFSSYEQTLLVIGFAVAFI
                     FAYLAVKIFLKIVENYGFKYFGYYRILIGLVFIIYAVR"
     gene            complement(35984..>37314)
                     /locus_tag="PLG98_04680"
     CDS             complement(35984..>37314)
                     /locus_tag="PLG98_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE32581.1"
                     /translation="RTTIGSFDIPEKYFDMLGIAKSSAGYTQLSLLASGKNYNAEDFF
                     ERLSRSGLAKIFDADLFNKRLGDFLKDLFLEIAIIISIGLVLITFLYFLDWQLTLAAM
                     APIVFALFATLGTMKIIGHPLDIPGIMLWIIIMGMGDDYSVYYICHYQRNVDEKSLAM
                     HTIKLAIFLAALTTLIGFGVLALADHALLRSIGIVSFLGIIYSLIGVFFILPSLMDKI
                     FAPIAYPADKVVIGSRDHLRRTVLRYRHLPGYPRVFARMKIMMDPMFRELHKYVKNPR
                     RIIDIGCGFGVPATWLLEIYPDARVFGIEPDVERVLIASRVIGSRGRVEAGRAPDLPH
                     VEGEADHVLMLDMLHLISDDEVKIVFQRIYDKLTADGTLLIRTTIPSDKPNPWKRWIE
                     TSRLKMTGMPNRFRSETEIADFMKQAGFAVQVFASDTRSIEEKWFAGKKY"
BASE COUNT         9379 a         9277 c         9425 g         9233 t
ORIGIN      
        1 tcgatggaat actcccaggc atcttcggaa aatcttgccc aggttaaatg cgacagcctg
       61 gtgcccaaat ccacccatga agccagtgcg ccgagcggct ctacgggggc ctctgatttg
      121 atttttttat tgcaggccat gtcggttgtc tgtgtcataa gttgatgcca tgatttgaaa
      181 aggtcgtaat gattcatttt gttctctcag gaatgtttta aggtgatcat gataaaaaat
      241 ctgtctctca ctttcaagca gtataggaaa ctcaccccgg gttgtcaatg aaatattaac
      301 cggataagtt attaaattta tttattttaa gccaatgccg gtttaatttt tttccggatg
      361 aaccgtagcg cgggaattga aggaaaccgg attcgttcgt tcttgattgc gtcaaaatat
      421 tcattgaagt ttttcctatt ttaacataac tgattgtcag cttgcatccg atttgagtca
      481 tgatccgaaa aaaggggagg ccgtagcgta cagtttccgt ttttttccgt gcatagaaag
      541 cttgtaggaa aaggaggtaa aaaaatgaat aacggctcaa ccaaaatgac ggatattaag
      601 atagacacca acaaccttta taaggaagaa gctttcaccg acctgacgtt tgccaccatc
      661 aggcggctca atcctgtgaa tctggacggc tcgcctgacg ccagccgcga accgattttt
      721 accggtatga cccagttgat gtcgcccaac ggtccgattc ctgttcaatg tcttatcgaa
      781 ggagcaaaga cgcttgccga agctgcagaa aaattaccgg tggccattga aaaaacggtt
      841 caggcgatga tcgccgaagc caaggaaatg gaacgtgagg aagcttccaa gatcgtcatc
      901 cccggtcagt catgaaacaa aatctgccgg acaaaaatcc ggcagattat ttgagtcttt
      961 tgtccattcc cgtaaaaccg caatcgggct gatagcaggt tacgttcaga aattcgcatt
     1021 tctgtttgca aaccggacac gtttccggtg gtgtaatttg caccaccgta ttgccgcaat
     1081 tactacactt ccaggtattt tcgggaagag gctgtttgtt ctctgtcatt gccgtatttt
     1141 ctccttatct gggttaaggt gattctatca caaaaaacag atagatagac attttttctt
     1201 tttcccgacg aatatgcttg atattattac ccactttcgg taaacgaata taccattttt
     1261 acccaaggca aaaacatgcc caaagaaaaa aagaaaaatg attttgcagg attaacactg
     1321 ctgggaaagg cggcaaagcc ggtgcgcaaa ctggagacat ttcccaatca caacgaaaaa
     1381 cgtgcctacg ttgtcaccct gcagacggaa gaatttacct gcgtctgtcc caaaacgggc
     1441 cagccggatt ttgccaaaat taaaatccaa tacataccgg ataaaaaaat tgtcgaatcc
     1501 aaatcgctga aactgtattt atggtccttc cgtaatgagg gtgttttcca cgaacatgtc
     1561 accaatatca ttctggatga tctggtagca gcgctgaaac cccgctggtg caaagtcagc
     1621 gcggaatttg ccgtgcgcgg cggcatcgga atcaccgttg acgcggaata taaaaaataa
     1681 ccttatgagg aaaaggaaaa tccatgggaa aaagaacgga tattaaaaaa gttttgatca
     1741 tcggttcggg cccgattgtt atcggacagg cctgtgaatt tgattattcg ggaacgcagg
     1801 cgtgcaaggc gctgcgaaac ctcggttatg aaatcgtgct ggtgaattcc aatccggcga
     1861 ccatcatgac cgatccgggg atggcggatg tgacgtatat agaacctctg aatatccagg
     1921 cattaacgga aattattgaa aacgaaaaac cggacgccat cctgcccaat ctgggcggac
     1981 aaaccggcct taatctgacg tcggaacttt acaaaaaggg cgtgctggaa aaacataacg
     2041 tgaaagttat cggcgtgcag gttgatgcga tcgagcgcgg cgaagacaga atcgccttta
     2101 aggaaacgat gaacaaactt ggcatcgata tgcccaaaag cgaaccggcc acctccgttg
     2161 aagaagcgga aaaaatcgcc acaaaaatgg gttaccctgt cgtggtccgg ccggcttaca
     2221 caatgggcgg caccggaggc ggcattgtct ataatctgga agagctgcgc ctcattgcca
     2281 gccgcggcat ttcggccagc ctcatcggcc agattctgat tgaagaatcg gtcatcggtt
     2341 gggaggaact ggaactggaa gtcgttcgtg acgcgaaaaa ccagatgatc accgtctgct
     2401 tcattgaaaa cgtcgatgcg atgggcgtgc acaccggcga ttcctactgc accgcgccga
     2461 tgctgaccat tactccggaa ttgcagaaac gccttcagga ttattcctac aaaatcgtcg
     2521 aggccatcca ggtgatcggc ggcaccaatg tgcagtttgc gcacgatccc aaaacagacc
     2581 gcgtggtcgt catcgaaatc aatccgcgta cgtcgcgttc ctccgcgctg gcctccaaag
     2641 ccacgggttt tcccattgcg ctgatttcct ccattctggc ttccggaatg acgctggatg
     2701 aaattcccta ctggcgggac gggtcgctgg aaaaatacac accctcgggc gactatgtcg
     2761 tcgtcaaatt cgcccgctgg gattttgaaa aattccccgg ttcggaagac aagctgggca
     2821 cgcaaatgcg cgctgtcggc gaagtcatga gcatcggcaa aaattacaag gaagcgttac
     2881 agaaagccat ccggtcgctg gagaaaaaac gttacggcct gggatttgtt aaagacttca
     2941 acgcgaaatc gttggatgaa ttgatggaaa tgctctccga gccctccagc gaacggcaat
     3001 tcatcatgta tgaggccctg cgcaaaggtg cttcggttga gacgctgtcc gccaaaaccc
     3061 atattaaaaa atatttcatc gagcagatga aggacctggt cggcctggag gaaagaattc
     3121 ttcagtataa aaagaaaaag atgcccgatg agctgctgac gcaggcgaaa aaagacggct
     3181 ttgccgataa atatctggca cagattttgg gcaaagagga agaggcgatc cgcaaacagc
     3241 ggcaggcgtt gggcgtaacg gaagcctggg aagccgtacc ggtcagcggc gtggaaaatg
     3301 cagcgtatta tttttccacc tacaacgcgc cggatactgt gcctgtaacc gggaatcgca
     3361 aggtaatggt tttgggcggc gggcccaatc gaatcggcca gggtattgaa ttcgactact
     3421 gctgcgttca cgcggcattt gccctgcgcg atgaaggtat cgaatccatc atggtcaact
     3481 gcaatccgga aacggtttcc acggattacg acacctccaa taaactgtat tttgaaccgc
     3541 tgacagtgga agatgtttta agcatctacg agaaagaaaa accggaaggg gtgattgtgc
     3601 agttcggcgg acagaccccg cttaacatcg cggcggaact ggccaaagcc ggtgtgaaaa
     3661 ttatcggaac atcgccggag acgatcgatc tggcggaaga ccgcgaccgt ttccgcatga
     3721 tgatggataa actgggcatt cccatgccga aatccggcat ggccagcaat ctcaagcagg
     3781 cgctgaaagt ggccggtgac atcggttatc cgctgatggt gcgtccctcc tacgtgctgg
     3841 gcgggcgcgg catggagatc gtacatgatg aggaaatgct caaacgctat gtcaacgcgg
     3901 cagtcggcgt cacgccggag cggccgattc tgatcgacaa gtttctggaa aacgccatcg
     3961 aagcggaagc cgacgcgatt tcggacggca cggatgcttt tgttccggcg gtcatggaac
     4021 atatcgagct ggccggtgtt cattccggcg attccgcctg tgtcattccg ccggtgagca
     4081 ttccgctgcg tcacattgaa actatcaatg actatacaaa aaaaattgcc gtggaattcg
     4141 gagtaatcgg cctgatgaac atacagtacg ccatcgccaa tgatgtggtt tacattctgg
     4201 aggcgaatcc ccgcgcctcg cgcacggtgc cactggtttc caaagtctgt aatgtgtcca
     4261 tggcgcgtct ggccacgcaa atcatgctgg gcaaaaagct taaggatctg aatctgaaaa
     4321 acaaaacgtt ccgtcacttc ggcgtgaagg aggccgtttt tccctttaac atgtatccgg
     4381 aagtcgaccc ggttctgggc ccggaaatgc ggtccaccgg cgaagtgctg ggactggccg
     4441 attctttcgg cctggcttat tacaaggcac aggaagcgac tcagcaaaac cttccctcgg
     4501 agggaaccgt tctgattacg gtggaagaaa aagacaaggg cggcgtgctg gaagcggcgc
     4561 gggcgtttaa aaaaatcggt tttcaaattc ttgcctcgga ggggacgcac aggtttctga
     4621 aagaaaacgg aatcgaggcc gaaaagattc ttaaaatgca tgagggcagg cccaatattg
     4681 tggatgccat caagaacggc gaaattcaac tggtgattaa cacaccggcc gggcgcctca
     4741 gcaaatatga tgattcctat atccgtaaag cagccattaa atataaaatt ccttatatca
     4801 cgaccatcgc ggcagcggtc gcggccgtca agggaattgc agcgctgaag cagggccacg
     4861 gacgggcaaa atccctgcag agctatcacg cggaaattca gtaggaatca ggctccgatt
     4921 tgagaaaata aagcttttat ttttcgacag ctcattatag gttaataata tataaaagaa
     4981 cattgtttat aaaaaagctg tgggcaaact tttaatgaga cacgggcgaa tatcttccgt
     5041 atatttacat tgacttatta aaattcttcc tttagcttca ttcacattat aacaatccga
     5101 tagaattttg agcggacatt gaggctgtat agacgacttt gcgtgaggag aaggaatgga
     5161 aagcaaggac ggtaaagaag ataaaaacgt taacgcctta ctctctctcc ctgacagtac
     5221 caagacgttg ggaaaaaaat ccaagtccag agaggtcaac ccttccggat taaagaggaa
     5281 ggtttcgcag gatacccgca ggatgccgca gaaagacaaa ccggcgatca gcggcgcgtc
     5341 cccaaagacg gccggacata aaaacgggaa ggagtctttt tgcggccctg aagaaaaata
     5401 cgcgctggcg ttcaaatata gtccgatcgg gatctgcata tccagcctgc aggatggccg
     5461 ctatgttgaa gtcaacgagg tttacagtaa ttttctggga taccgccgcg aagaactcat
     5521 cggacgcacc tcttcggaaa tcaatctctg ggtggatatg gccgaacgcg acgcactggt
     5581 tcgggaactt cttgaaaaag gcaccgtgac ggattttcaa ttgcggtttc gtgacagaaa
     5641 cggcaacgtt cactggggaa atgtggccgc cgcactggtg accatcggac aggagttgta
     5701 tgttctcaca cagacccagg atgtcaccga taaaaagaag atcaaagaac agtttgagaa
     5761 aagtgaggcc aagtaccgtc tgctggcaga aaatctgacc gacgtcatct tcaccatcga
     5821 catgaatttc aattatacgt atgccagtcc ttccacgtat catcttcttg gatacacggc
     5881 agaagaattg atgaccatga aagtggagaa tcttgttgat cctgaaacgc tcgcctggtt
     5941 catggagatg ctggctgagg agatggaaat agaaaagcgg cccgaccggg atttgaaaag
     6001 atcgcgggtt cttgaatatc agcatatccg caaggatgga tcaacgatat gggtggaaaa
     6061 caagataacg ttcttaagag atcaagacaa taatgcgata ggaatcatcg gaaccgtccg
     6121 cgacattgcc gaacgcaaac gggcggaaga gcaggtttgt aaagaaatcg atttcaaccg
     6181 ttccgtactg gacagtctga cggtcccttt cttcatgttt gataccgcca gtatgacttt
     6241 cttcagatgg aacaaaacct tttccgctgt ttccggatat tcggatgagg aactcggcca
     6301 aatgagaccg tcggctttga tatcggaaca ccagaaagat tttttattgg ccaagttcga
     6361 ggaatttcaa agaacaggcc atatcgcctt tgaaatgccg gttctttcca aaaacggttc
     6421 tgtaacaccg tatttgcttt cgggcaatct tctgcatcac gacgggaagc cttttgtggt
     6481 gggtatgggg attgacatta cggagcgcaa aaaagcggaa gaatcgctgc gccggagcga
     6541 ggaaaaatac cggaccattc ttgagaacat ggaggaaggg tattttgaag tcgacctttc
     6601 cggcaatttt acttttttca acgatgccac ctgccggcat ttcggctatt cccgcgagga
     6661 aatgacggga atgaaaaacc gtcagtatat ggacgaaaaa aattcaaaag cgctttatat
     6721 tgtttttaat cgtatttacg aaacggatca gccggtaaca aatctggaat ggcagatcat
     6781 aagaaaagac ggcgccaaaa gagatatcga aggttttgtc tttctgctga aggatgttgc
     6841 aggcaccccg ataggtttca gaggaattgt ccgggatatt accgaacgca aacaggtgca
     6901 ggaaaagctg caccgcgaaa ggcagttatt caaatctgtt acggaacaat cgtccgacat
     6961 cattattatt gccgacacgc gaggaaggat cacttacgaa aatccggctg ttgaaaagat
     7021 actcggatac cgtgttgaag aaaggctggg ggcatcggga tatgacctgg ttcatccgga
     7081 tgatgcgcaa atcgtcgctg atgctgcggg gaaacttttc cacaatatcc aagcgcctgt
     7141 gcagaaaggc gaaatacggt tgcatcataa aaacggaagc tggcggacgt ttgaagtcac
     7201 ggccagcaat ttggtcaagg atcatgtcgt ggaagccgcc attgtcaata tgcacgacat
     7261 caccgcgaga aaacggtcgg aggaactcat caggcaaagt gaagcaaaat atcgtctgct
     7321 ggcggataat atgaaagatt acgtgtggct gatggatctg aacctgaaaa tcaattatat
     7381 cagcccctct gttgaaaggt taatgggtta ttcgcttgaa gacttcaaaa aattctctct
     7441 cgacaaattt ttgaccgtac aatctttcca ggcggcgatg gatttctttg ccattgaaat
     7501 gcctaaagct ctgggagctc ctccggatta tgttttggag cggtctctgg aacttgagtt
     7561 ttgctgtaaa aacggccaaa ccgtctgggg agaatgcaat ttcagtttaa tcagagatga
     7621 caaaggcaaa ccgttgtccc ttctgggggt cagcaggaat attaatgaac gcaagcaaat
     7681 ggaagatgct ctgcggaaaa gcgaagaaaa tttccgtcat tctctcgatg actctccgct
     7741 cggtgtccgt atttcgacgg ttgaaggaaa aacgctttat gccaacaggg ctatcctgga
     7801 catgtacggg tatgacagca aagaggaatt gcagaacacg ccgctgcagg agcgctatac
     7861 gcctgagacg ttcgaagcgt tcaaagaaag aaagaagaaa cggctgaagg gccagttcgg
     7921 tccgtccgaa tatgaaatca gcattatcag aaaggacgga gaaatccgcc atctgcatgt
     7981 tttccgcaag gaaattttct ggaacggcca gaaacagtcc caggtgattt atgaggatat
     8041 aacgcttcgc agacgagcgg aaaacagact gaatgaaacg ctggaaagtc tgcgtcaatc
     8101 catcaaaata accattcagg tcctggggac ggcatcagag gcaaaagatc cttacatggc
     8161 cggccatcaa agacgggttg ccgatttggc ccgtgctatt gccacggaaa tgaaacttcc
     8221 tcaggataaa attgaggcga tacgaatggc cagcgccatt catgatatcg ggaaaatttc
     8281 ggttccggca gaaattctct gtaaacccgc gattctcagt gatatcgaat tttcttttgt
     8341 gaaaaatcat ccccgctaca gttatgacat catgaaagaa gttgaatcgc cctggccttt
     8401 ggccgatatt gtttatcaac accatgaacg catggatggt tcgggatatc ctttgggact
     8461 gaagaatgac gaaattctca tcgagtcccg tattctcgcc gttgccgatg tggtggaggc
     8521 catgatatcg taccgtccct acagaccggc gctggcgctt gaaatggcgc ttgcggaaat
     8581 tgaagataat gcgggcactc tctatgacgc cgaggtggtc aaagcctgcc tgagattatt
     8641 tcgggacaaa aattatcaaa tgtcttaaga tgatatatac gtaccggata caaattgaaa
     8701 aaacggcgat tccacggata cgcttgacgg atcgatggat tgttttataa tatcccatga
     8761 tttatgaagg aggttccctg tgaaaaaact tacggccatt attttgtttg tgctttgtct
     8821 gatcccggtt gtgatatttg ccaaaaacgc atcggtggag gaccagttca aaaaaagttt
     8881 tccgaaaaac aagtttgaaa gtatcgtacc cacatccatc aagggaattt atgaagtgta
     8941 tagcggcaat cagctttact attacatgcc gaaggacgag atcgtgcttt acggcaccct
     9001 ggtgtccaaa agcggcgtca acatcacccg tgatagtctt ttaaagaaga tggcgcagaa
     9061 gatggagaaa ttgcctctgg agaacgctct caagatcggt aacggaaaaa aaatcattgt
     9121 tgaatttatg gatccccatt gttaccattg ccgtcaggcc tatcagttct tttcccagcg
     9181 caaagatgtg accatgcatg ttttcttttt cccgctgtcc aaagaatccg cagatcaaat
     9241 caaacatatt tattgttcca aggacgtttt aaaaacatat gacgatgtca tgaaaggcaa
     9301 gctcgacaat aacgccagtc tgaatgtgtg cgcggatgcc aaaatcgatg aaaaaataaa
     9361 gacgcatgtg cagcttgcgt cgcaggtcgg actgcgggga acgcctttat tctacattaa
     9421 aggccaggtt gtggacggat ttgatgttgc gctgatcgag aaacttctca aagactgatt
     9481 ttcataaggc cggccgctct tgctgcggga gagcggctag cggatttttc ctcctgtttt
     9541 tgccgcgcgg tcgaacgtca gaagggttaa tccttctggc gtgtcggtgg ccagcagcat
     9601 gcctcgggat tctaccccca tcaactttgc cggttttaaa ttcgccacaa tcacgatttg
     9661 ttttccgata aggtcttccg gtttgtaatc cttgccgatg ccggccacga cggtgcgttc
     9721 ttcgccgatg tccaccgtca gttttacaag tttgttggat ttgggtacgg cgtgagccgc
     9781 gagaatcgtt gcggcgcgta aatccacttt tgaaaatgtt tcataatcta tttccggctt
     9841 gaggtcggtc atggctgctt tctgatcctc gggtttttct ttttgctgaa caatacgcgg
     9901 gaacagggca tcgctgcggg tcaggacctg gcctgttttc aatgcgttgt ccgttttgat
     9961 gtcgtccagt ttcagacctt cggttttttc gcatccgatc tgtcggagaa tcttttccgc
    10021 cgtttgcggc atgaaaggcg aaagcatgac ggcgatcgcg tgcagcgccg aaagcagatc
    10081 atacatgacc gccgttaatt ttttaccgtt ggccgggtct ttggccagcg accaaggctc
    10141 gttttcgacg atgtatttgt tggtgatatt aatgaaatcc cagacggcaa tcagggcttt
    10201 gtgcagagac atatccctga aactcgcctc aacgtctaag acggctttca aagcggcctc
    10261 gcgaagggcc ggattttttt catcgcctgc aacggccggc actctgccgc cgcaatattt
    10321 gatggccatg gtaatggcgc gactgaccag gtttcccaga tcgttggcca gatcggaatt
    10381 caggcgaccg acaaaagcat cctcggagaa attggaatcc agaccgaaaa ccatgtccct
    10441 taaaagaaaa tatcgaaacg catccagacc gtatttatcc ttcaaatcca gcgggcggac
    10501 gatattgccg aggcttttgg acattttgct ctgatcggag ttccagtacc cgtgcacgtt
    10561 caggtgctga taaggttcaa tacccgccgc tttcagcatc gtcggccagt agatgccgtg
    10621 cggttttaag atatccttgg cgatgaggtg ttcggcgacc ggccagaagg tcttgaaatg
    10681 ctccccctcg ggatacccca cggcggtgac gtaattaatc agggcatcga accagacata
    10741 ggtaacatac tgctcatcaa acggcagggt aatcccccat tccagtctgg ttttggggcg
    10801 ggaaatgcaa aggtcttcca ggggatcgcg taaaaaagcc agcgcttcat ttctgtatct
    10861 ctccggccgg atgaattcgg ggttcttttt gatatggtcg atgagccagt cctgatattt
    10921 gctcattttg aaaaaataat ttttctcttc aatatactga ggttcccggc cgtgttccgg
    10981 acatttaccg tcgacgattt ccttttcggt gagaaatcgc tcgcagccga aacagtagta
    11041 accgccgtaa ctcccgaaat aaatatcgcc ggcatcgtac accttctgaa gaatatagcg
    11101 cacggtttcg atgtgattgg cgtcggtggt tctgatgaaa taatcattcg agatggcaag
    11161 ttgcggacaa agggcgcgaa actgggcgct gatgttatcc gcgtattgtt tcggggttaa
    11221 tccggctttc tgcgccgcct cggcgatttt atcgccgtgt tcgtcggttc cggtaacgaa
    11281 aaacgtttta tctccgatca tgcggtgata tcgggccagc acatcggcga caatggtcgt
    11341 ataagcatgc ccgatatgcg gtgaagcatt aacatagtaa atcggtgtcg tgatataaaa
    11401 tgatttcggc atatgtgacg tttctccatt catgactgaa gatgatcaat gtcatccatc
    11461 ttttccagat ccagcgtttg cttttcttcc tgtgcttcag tggcgctgcc ggacgttttg
    11521 gccgctttgg cggctttttt ctttccggca tagccgtcgt attcgaaggc caggcagcac
    11581 atcagccttc cgcacaggcc tgaaattttt tcaggattca gcgacatgct ttgttccttg
    11641 gccatcttga ccgtgactcg ttcgagattt tgcaggatgc cggcacagca caccatccgg
    11701 ccgcaaattc ccagtccttt ggtaatccgc gtttcctgac gggcgccgat ctgtttgagc
    11761 tcgattctcg ttttgaactt ctggaccaga tccttgacaa gttcccgaaa atccaccctg
    11821 ctgtccgctg taaagtaaaa aataattttg tttttttcaa aaaggcagtc gacattcacc
    11881 agtttcatgg aaagctcgcg tttgcgaatt ctttccagac agaatttcca ggcctcctgt
    11941 tccagcgcgt cattctcttt tctgatcttt aaatcgtcgt ccgtcacctt gcggattatt
    12001 tttttgagat tggccggaac ggccgacggc tcgcatcgct tcacatccgt gacgacagtg
    12061 cccagcgcca ggccgctgtc ggtttcgaca agcacctgtt catcccggtg caggataaga
    12121 tcggcggcgt caaagttata gatccggcct tctttcttga atttaactcc aatgatattt
    12181 acaagcaatg tccatccacc cttttttaat aattcagctt aaatgccatt gtttccaatg
    12241 tcaatgattt gttgacgttt tgttcgatga tgtcccccgc tttttctatc tgcgcaatgt
    12301 tttgcagaat ctgctttccg gtcaatctgg ccgcgcaggc cgcaatcaga ggcgtattgt
    12361 cctgattgat cagcattcct ttctggccga cttccttgaa aaccagcaca tcgcgaaaga
    12421 acgtgttcag aatattcagg ctctgccgga tttccttttt tttctggccc agaaaagagg
    12481 caaaatggat caggctgaaa ggctctttcc ggtcggtcag ggccagcgtg ttgaacagtt
    12541 cggtccggta cgcgacaact tcctcttggt tgagctccag cgcgttgccg attgttccgc
    12601 cggataacgc ggccagcagc attgcccttg aatgctccat gtttttttcg gatacgagaa
    12661 accgcgccac cgtttcgacg gccagcggat taaagtggat gtgctggcag cgggaaatga
    12721 tggtctgaag catagcgtgc ggcctcgttg tgatcagaat cagcatattg gccggcgacg
    12781 gttcttccag cattttcaaa agggcattgg cggattcctc attcattcta tcggcatcat
    12841 caataataaa agctcttttt ttggcctcca gcggccggca ggtggttcgc tcctggatct
    12901 gacggatgga agcgatgcgg atgaactggc cctcgggttc cacaaaaaaa atatccggat
    12961 gactggcatg ttgaattttg cggcaggacg gacaggcatc gcaggcatca tgccggtcat
    13021 ccgaacttat gcaattgacg gccttgacga attccacagc cagcgttttc ttgccggtcg
    13081 ccgaaggacc gctgaagaga taggcgtgcg cgattcgccg gcgggcgagg gttttgcgga
    13141 gcatgtcaat ttttttttcg tgaccgtaaa tattgccgag cgacataatg cctgaaaaaa
    13201 ttcttttaaa aagatgattt tagaccttga taaaactgct tatgtgacga cgcacctctt
    13261 cctgaatgac cggaatcttc cgggtggcgt caatcaggcg gaaacgttcc ggttcattat
    13321 ttaaaatagt cagataccct tcacggatgc gacggtgaaa atccagagtc tccctttcaa
    13381 accggtcggt ggcggccggg tcggcgagtc gattgttgcg cgccgttgcc ctttccagtc
    13441 cggtttccac cggcaggtca aacaataagg tcatgtccgg tttcaactgc atcgcggaat
    13501 aatcattgac cgcgccgatg aatttctgat cgaggccgcg tccgaaaccc tgataggcgt
    13561 aggtggcgtc ggaaaagcga tcgcataaca cgagtgtccc ctgccggagg gcgggtagaa
    13621 tgacgtcccg gacatgttgg gcccgggcgg cgcagaataa aaacatttct gcttctgcgc
    13681 acagatgatt gtgcctgcgg tggaaaagaa tgccggcgat ctgtcgcccg atgtccgttc
    13741 cggaaggttc ttgcgtgaca atcagttcaa cgccctggtt ttgcagatat tcggcgacga
    13801 gcttgatctg cgtcgatttt cccgaacctt cgataccctc aaaggtgata aatttttttg
    13861 ccttgatcat gtgactccgc tttcacctgc gcaacggcgc aatgaaccgg taaccgtgtt
    13921 ttaaaaaata aaaccgggaa tgaatcaatg cgtaaaagca gatttattcc cggagctgat
    13981 ttttcgtctg tgaattattt cccctctccg ggaacgatcg cttctaccgg acactggtcg
    14041 gcgcaggttc cgcagtcatt gcacagttta ggatcgataa catacttgtc atcgccctct
    14101 gagatggctc cttccggaca ttcgctttca caagaaccgc aagctataca ttcgtcagta
    14161 atcacatatg ccataatttt atgctccttt tatcgttatt ttaaaataca cctttcccgc
    14221 tttgtcttgt caggaaacga ttgttcttaa aaagccactt tcccgttgct gatgtgcgaa
    14281 acggctcaga cggccacgat ctctttaact ccggggacca gtttcttaat gactttttct
    14341 attcccattt ttaacgtcat ctgcgccatg gggcatccgt ggcaggcgcc ggttaacctg
    14401 accttgactg tgccgtcatc gctgacatcg accagttcca catcgccgcc gtccgcctgc
    14461 agactgggcc tgatggtatc aatggctttc tgtacgtctt ctttcatgga ggttctccct
    14521 attccgtttt ttcttatttt tcaagtcctt cttttcaatg cggcatgtaa taaaccattt
    14581 tgtttgcgac atcccgggcc atatcaggca aacccggttt tattaataca taatccttct
    14641 tgatggtgtg attggctttc cacattaatt tgccgttcgt aacatcatac agctccattg
    14701 tcaaactgac ctgcgccatt ttcttatctg cctcaacgaa atatttccat tcgtcaacgg
    14761 ataacagcag aataccgtca acctgcagga tgtcaccgat tttcctactt aaatcagcat
    14821 cggaaaactt cagaagttgc agtttggaaa aatactcttc cgctgttttt ttcaatagtt
    14881 catcattttg catctgtttt tgcagttttt ccgcgtctgt gacagaagta aacaatttct
    14941 tggcaatcag tgagccggcc acaatctgtt ggaccaccgt tctgttatcg ggttctttgt
    15001 gattccagac ttcaatcgga aaaacagcaa tactctgcgg atgaaaatcg atggcctccg
    15061 gcgccagttg agaaaagtgc aaaccgccgc aaccggcaat ggttagaata agggcaatcc
    15121 atacaaagac ataagatttt tttttaatca ttgaacaaaa accgcctcgt catcagatca
    15181 gaatagccgg tgtcagaaaa tcagccacag caaaacacaa aaaccagcta taatgtagct
    15241 ggtttccaga aggccttcct taaccgctcc agaaagcacg ggtcttttat cacaaaacag
    15301 gaaacaaatc cacagatata ataaacaaaa catcagcgga aaactcaggg atgacgtcca
    15361 tcctgaaggc gatgcaaaaa gtaaaatagc gaataatatc aatgagataa tcttcaggat
    15421 attcaaggcg cgtgttttac cgaacagcac gggaaacgtt tccttgccga tcagtttgtc
    15481 gctttgtatc tccagaatgt ccgacatggc tgaacgaata aaaacgatgc agaaaacaaa
    15541 gaaaaatgca accaaaaggc cggcatccgg aaagggggtc tcatggatag agggtaaaaa
    15601 cgcagcggcc accgcccagg ctatggacat aaatatgttt tttgaaccgg gcacatcttt
    15661 aagactttgg aagttccacc ttggggaaag cagttgcata ttataaagca cgccccccag
    15721 tgaaatgatc agcagggaaa agaaaacagc cagcgattgc tgaaaggcca gtaccagcgc
    15781 aagaataatg gaaaataagg aagtgacaaa aaatatattc tcgtgccgta tataatattc
    15841 ttctctgaaa gatccgacga gccccgccgg tttacggctg atcagacggt ttaagacatg
    15901 catgccgtaa acgaaaagag aggcaatcag aataaatgaa aaacggacgg tcatggtctg
    15961 cagcaacatg cagacaaggc acaggcaacc ggcgccgatg gcggagaaaa tatccgtttt
    16021 aacgaggaat gtccaaagag tgagcagttt tcccagattt ttcaacttac ggcttcgtcc
    16081 ttccgtgatt ttgtctatga ctctgtccag aatccagttg ggcgtggacg ccccggccga
    16141 aacaccgatt ttattatacg ggccgaggtc gattttttcc atatctgccg gtgtttcaat
    16201 atgaaatgtt gagggttggt ttttgcgggc gagatcagcc aaccgtttgg tattggcgct
    16261 gtttttaccg ccgacgatga acatcgcatc catctcaccg gccatgtcaa tcacttctgc
    16321 ttgtctctgt tcggtggaca aacaaattgt attgaacacc accgcctgcg gacagagttg
    16381 tttgatcttt tcgacgatct tttgatactc gtcaaggttt tgcgttgttt gggcaaccac
    16441 gcagaccttt tccaggcaag gaattttatt cacttcctct tctgtggaaa taataatgcc
    16501 ccggccgtcg gtatacccca gcaggccgtc gacctcggga tgttccttat ccccgacgat
    16561 gacaaccgtg tatgtctgtg cggcatgttt tttgataatg gactggacgt aaccgacctt
    16621 cgggcacgtt gcgttcacca ctttaatgcc ggtttcttta attttttttc tttctgccgg
    16681 ggcgatgccg tgcgcgcgta tgatcaaaat ggcattttct ttatcttcga tttcatcaat
    16741 atgggtaatc ggtttgatgc cgcggctttt cagcagttcg atcgtctggg gattgtgaat
    16801 cagcggcccg taggtataga tgcggcggtt tttttcatgc tgggctatgt ccagtacgat
    16861 atccacagcc cggcgcaccc ccatgcaaaa gccggcagtt ttggccagtc taatttccat
    16921 attgctttgt ctttcgcgct attttgttgt tgtacgatat ttgatcatct tcctgaaacg
    16981 agtccggatg accctgacgt tgccgttttt ctgccggccc ggatcgaccc gagcggcagg
    17041 acaggaatcc ggcaagcgcc gccaacgaaa aaacgcctcc cgtcataatg gtgacgaata
    17101 tgattcccgt gttcgcgaga accatccatg atttgatgct gccgaccgta atgatcgagg
    17161 cgtaaatcag acacggcaga aaaaaatcgc ggtcgcgggc cgcaaagcgg tttctgtttc
    17221 taaaaaatat cattctatac gggataaaga tcataatcgt cgtcagaagc catccgaaat
    17281 agttctgcgc cggaatgccg tagaagacgg aaccggacgg cagtgcttgc accgtcgtcc
    17341 ctgtccatgt tccccccgtc acccacaagg gatcgatgag cacattcagt gcggtcattg
    17401 ccgcggcatc gaaaaacgca aacaaaaacg gggcggaccg gcctgcaaac cggtcaccga
    17461 aagccgcatc aactgtgacc gttgaaacat acatgaacat aaaccaggaa aacggtatca
    17521 gcagcggaac cgtatcgaaa atttttatcc ccagcgcgtc gccgtaatgg tattccccgt
    17581 aaataaggcc ggttttcact cctgtgtatt ccataataaa agatatgaca aatgtaagga
    17641 cggccagaga gatgatacat ttcctgccga cgtatgtacc gccgtgaaga agcgcaaagc
    17701 acgtgaagac cgcccccatg gccatattat aataaggcgg cgacgttctt cccgacgcgg
    17761 tccataaaag ctcagaatag gtggaaaaca atacgatgat gaatagcaca taaatcaaat
    17821 gtttattttt catcagcttc cttttccttc tttcgtgtca tcgtctgacc tgcacattgt
    17881 caggtttgag acggtttttt ccgcgttaag acgaaaaatt ccttgtttcc cgccgggccc
    17941 gaaattggcg aggcgcaggt gccctcaacg tccagatcca acgatatgca aaaatcgctg
    18001 atgttttgca ccacgcgatc atggattgtg ggatcccgga caacgcctcc ctcgccgacg
    18061 tctttttttg atgcttcaaa ctgcggttta atcagcgcca gaatccgtgt gccttcttcc
    18121 agaaatttta aaaccggagg aattacagtt ttcaaagata taaaagatgc gtcgatcaca
    18181 gccagatcaa ttttttcatc cagatccgca ccgtcataat agcggatatt ggttcgttca
    18241 atgacgacaa cgcgcttgtc ttcgcgaagt ttccacgcca gctgaccata agcgacatcc
    18301 agcgcgtaaa ctttccgggc gccggcctgc aacagacaat ccgtgaaccc gccggtggaa
    18361 gcgccgacat caaaggcgac caggccctca acgttcatat caaattcctt gagcgctccg
    18421 gccagtttca atccaccgcg gctcacatag ggattatctt cccccttgat atggatttcg
    18481 gtatggggaa cgaacaatgt cccggccttg tccggatagg ctccatccac tttcacagcg
    18541 cgggctaaaa tcagggcgcg tgccttttcc agggtaggcg cgattccgcg ttcaacaagt
    18601 agtttgtcca aacgaatttt ctcggcagcc atagaggatt gttgatgtcc ttcaagtcaa
    18661 gaagtcgagc aggctcaaga cggtcgtcgg aatgattcag acgtcagaat ttcgttgatg
    18721 gcggccataa tcccttcttc atcgattcca taccgtcggc gttgctgcgc ctgcgttgca
    18781 tgtctgacga attcgtccgg aatgcccaga cgttttaccc ggatgccggt gatgcttttt
    18841 tcagccagca gttccagaac agcgcttcca aacccgccct gcagtgtgtt ttcctcaacc
    18901 gtgatgatgg tcccgacagc ggccgccgtt ttcagaatca gttcttcatc caggggtttc
    18961 acaaagcggg cgttgatgac gctgatatcc cgtccctctt cggcgaggcg gttcgccgcc
    19021 gccagagcag gcagcaccgt tgaaccgatt gccatgacgg ccagatcggt tcctcccctg
    19081 agcacttctc cctttccgat gtcgagagcc gtcagaacct catccagggg tctgcccgct
    19141 ccgctcccgc gcgggtaacg cagagaaacc ggctgtccgc aatcgacggc ggttttgagc
    19201 atatgctgga gttcgttttc atctttgggg gccatgacca cgatgtgggg aatggaacgc
    19261 agataggagt aatccagaag cccctgatgc gtggcgccgt cctcaccgac cagtccgccg
    19321 cgatccacgg caaagataat gggaagtttt tgcaggcaca catcgtggac gatttgatca
    19381 taggcgcgct gcaggaaagt tgagtagatg gccacaacgg gaatgaaccc ttccgtggcg
    19441 aggccggcgg caaaggtgac cgcatgctgt tccgcaatgc ccacgtcata aaaacgattc
    19501 gggaattcct gactgaagag attgaggccc gtgccttcgc gcatggcggc ggtaatcgcc
    19561 acaatccggg aatcggtgcg cgcgagcttc accagcgtcc tgccgaatac gtttgtgtaa
    19621 gtggggatgc cggacgagga agcaatgacc tggcctgttt gcacatcaaa aggggcgacg
    19681 ccgtgataac tcggcggatt ctgctcggca aaactgtagc ccttgccttt tttggtaatg
    19741 acgtggacaa gcacgggacc ggtcatctcc cggatgtttt cgaacgtttt aatcagatga
    19801 tcgaggcgat gtccctccag cggtcccacg taggtaaaac ccatttcttc aaagagcgct
    19861 ccgggcacca tcagggtttt cagaatttct tcgattttct gggcgaattt gatcatatct
    19921 ccgcccacgc cgggaatgct tttgagaaat cttttgatat ccgctttcag ggtggtgatc
    19981 ttatgaccgg tcatcacccg gttcagatag gaggacatgg cgccgacgtt ttccgagatg
    20041 gacatttcat tgtcgttgag cacaatgatc atgtttttct tgcggtctcc cacccagttc
    20101 aggccctcaa aggccatgcc tgaggtcatt gatccgtcgc cgatcacagc aatgatttta
    20161 tagctggcgc ccttcaggca ggcggcttcg gtcatgcccg cggccgcgga aatggaggtt
    20221 ccgctgtggc cgacattaaa cacatcgtag gggctttcct cccgccgggg aaacccgctg
    20281 atgccgtttt gtttgcgcag gtttttaaaa tcctctttcc gtccggtgat gattttatgc
    20341 gcgtacgact gatggccgac atcccagata attttatcct gcgctgtgtt gaaagcgtag
    20401 tgaagagcga gggtcagttc gacggttccc agcgacgcgg ccagatggcc gccgcacgat
    20461 gcaaccgtga gggtaatcaa atccctgatt tcctgagcca gcgtattgag ctgggcaagg
    20521 gtcagtccgc gaatatcggc cgggaaattg acatccttca gaacactgta atgaacggtt
    20581 tgcggttgct tgaccaaaaa aaatcctttc gttttttatg acttcctgtc cctgatgtaa
    20641 cgggcgatga caatcagcgg catggctctt tcatcaaaat cggccagact ggtgacggcc
    20701 gcttccacgt gctggttcag cttttctttt gcctgttgca gtcccatcag cgaaggatag
    20761 gtcatcttgt tgcgggcggc atcgctgccg gttcccttgc ccatcaaccg gctgtctcct
    20821 tcaacattca gaatgtcatc tgctatctga aaagccagtc ccacatgcat gccgtattcg
    20881 gtcagcgctt gaattttatc tttgcctgca ttgaacatca ccgcgccgga ttttacggcg
    20941 gcgacgatca atgcgccggt tttgcgccgg tgaatgtcct gcagaattgt ttcatccgct
    21001 ccggtttttt ccgatatcac gtcaagcgcc tgaccgccca ccatgccgtt gatcccggcg
    21061 gcatttgcaa tttcctgaat aatggccagt cgccgttccg cggagagcat gaccttttcc
    21121 gcacgggaca aaagcacgaa cgcctccgtg agcagtgcat ccccggccag aatcgccatg
    21181 ttttccccga aaactttatg gcaggtgggt ttcccgcgcc ggaaatcgtc gttatccatc
    21241 gacggcaaat cgtcgtgaat cagcgagtag gagtggatta attccagggc gcacgcaacg
    21301 gggatggccg ggaccagatc gccgccgacg gcttccgcgg ccgcaagaca gagaatcgga
    21361 cggatgcgtt tcccgccgtt gaaaacactg taatgcacag ccttgtaaat gtcggaagga
    21421 aatccgtcct cgggaagata acgctgcaga gcttcttcaa caatgttctg tctgtcctcc
    21481 agatacgcct tgaggaacag atcatcatcc tgcatcatgg tcgtctccct gaaaacgttt
    21541 tacctgcaag gtgttttcct tctccagaag cagttcaacg cggcgttccg tttcttcaag
    21601 cttggcggaa cagaagcgaa tcagcttgat gccttcttca aaggacttca aggtctcatc
    21661 cagaggcata tcgcccgcct ccattttctt gacaatatct tccagttttt ccagtgcgtc
    21721 ttcaaatttt tcttttgcca tcattgctcc tgaccccggt aaacgggaga cgttctttca
    21781 tgaattcgtt ccactgcctc aaacccgttc aaactttttg acggattcaa ggatatgctg
    21841 tctaacagaa aacacatctc gactcactaa aatatttttt gtattttagc gttaaaattg
    21901 ccttttgcca acaggacatt gacctcatca ccttcatgca ggtcccgggt ctgccggatg
    21961 agcgtgcctt cggggaccgt gcgcgtgagg ctgtagcctc tttccagaac cgcaagaggg
    22021 ctcaacgatt ccaggaccgc agcatccttt tgaaatcgtt ccttcaatgc cgtcagaaca
    22081 caggacaggc tgctgtgaag ttctttccgg agatttttta atacgatttg tttttcgttg
    22141 atctgctgct ccggactctg gtgtttcaga cgcagctcca ggtggttcag cttatggagc
    22201 aggatttgcc ggcgatgacc gaaagcggcg gaaaggctct ctttgagatc gtcaattctg
    22261 attttctgag tgacgaccgc caggcgcggg tctttaaatc tttcctgcaa cccctccaga
    22321 tgttcttttt cagccttcag atgacgcaag cagccggcag tcattctacg ggccagcata
    22381 ttaaccttaa atatcagttc cgttcggtcc ggcaggacga gctccgcggc agccgagggc
    22441 gtgggtgcgc gcaaatcggc agcgaaatcg caaatggtaa aatcggtttc atgccccacg
    22501 gccgatacaa cgggaatgtc cgaacggaaa atttcgcgag ccagcctttc atcattgaaa
    22561 gaagccagat cttccagcga tccgccgccc cgcgcaagga taatgacgtc cacctgcccg
    22621 aaggcgtgga aacggcgcaa cgcctgaatc atgtcgtttg ccgcgtcatt gccctggacg
    22681 cgcaccgggg caattaaaat gtccgttgcg ggaaaccgcc gccggctgac attcagaata
    22741 tctctgatca ccgcgccggt gggagaggtg atgacgccga ttctttccgg cagaaaaggg
    22801 agcgcttttt tatattgttt gtcaaataag ccctctgcgg aaagtttggc tttgagctgt
    22861 tcgaaagcct tttgcagggc gccgacaccg agtggctcca ccgattccac aacaagctgg
    22921 tattcgccgc gcggcggata aagagtcagg cgcgcccggc ataaaatctt cagcccttcc
    22981 tccagttcaa aattgacacg gcccccggtt cttccgaacg gacgaaaaaa aaccgcccgg
    23041 atctgacttt tgtcgtcttt gagtgtgaag taggtatgac cggattgcgg tcgccgcaaa
    23101 tcggctactt caccttcaac cagaagggag tcaaacgatt cttccagaag agatttaatg
    23161 gtattattta attctgaaac ggtcagaatt tgttccatat ggctaatcct gaagctgaat
    23221 tatcagatat tggaaggtaa aacaaggttg aattaaaagg cctggttctg taataaacaa
    23281 aatttcaaac cagcgaaggc tctgcacaaa attggatgat gtccatgatt ttattgacag
    23341 gcaaaaaact tccttatatc gtttacgcat ggaaagaaaa atgttttgga aaaagataaa
    23401 gaacgcagtt tgtccggtaa agcatattct gcgtgtgccc gtggggcttt ttattgttgc
    23461 attggtttgc gcggatgccg gtgcggccca aaataatgac cggttgattc aggcagcgga
    23521 aaaaggtctt ttgcaggacg ttcagacggc gcttgcccag ggtgccgata cgaacgccaa
    23581 atcggttaaa aagtattata acattttcgg caaggagata acgccgttga ttgccgcgtc
    23641 ggaaaacggc catgcggaaa tcgtcaaact gcttctgcag aaaggcgccg atcccaactt
    23701 ccagaggctt ccggaaagaa ccaccgccct gatgcttgcc gcggaaaacg gtcatgcgga
    23761 tgtcatgaaa atactgctgg aaggcaaagc ggatattaat gcaataaaca acaccgggat
    23821 aacggcactg atgctggcct cgaaaaacgg ccatccggat gcggtcggta ttctgatgca
    23881 ggggaaagtc gatgtcaaca cccgaaacaa tgacggcgta acggcgctga tgctggcagc
    23941 gcagtacggt catcacgaca tcgtccagcg tcttgtgcag caccaagcgg acgtcaagat
    24001 caaaaacaac gacggcgtga cggcgttgat gctggcgtcg aaaaacggtc acgcgaaggt
    24061 tgttgagcgt cttttggaaa cagatgccgg cgtgaatgtt cagagagctt ccgacaatgc
    24121 gacggcgctg atgatggccg ccgaaaacaa tcacagggaa atcgtcggac tccttctgaa
    24181 taaaggggcc gatgtcaatt tgaaaacaaa attcatcggt gcgaccgccc tgattttgac
    24241 ggcggcaaaa ggccatgcgg atgtcgcccg tctgctgatc gataaagggg cggatatgaa
    24301 tgcgaagaac aaagacggca ttactgtgct gatggtggcg tccctgaacg gccaccgggc
    24361 cgttgtgcaa ctgctgctgg ataaaggagt tcccgtgaat ggcaaaagta atgaaaacat
    24421 aacggctctg ctgatggcgt cggaaagagg acacaaggac attattcaga ttcttctgga
    24481 caagggggcg gatgtaaatg caaaagacag cgatggtttg ccccccgtgc ttcttgccgc
    24541 acagaaaggc cataaggacg tggtgactct tctgctggat aaaggagcgg atggcaacgt
    24601 cagagcgaag atcaaagata cggaatatac gttactgacg gctgcggaga tcaataaatg
    24661 gaccgacatt gtggaaaggc ttaagaaatc cggggtgaaa gaataatgcc tgcttccgtc
    24721 tgctgaaaac tccgagcaga ggcaactgca cctgccttgc ggccgttgca aacttttttc
    24781 caaaatcagt caatacggac cggccggggc cgtcggaatc gtttaccaga taaaaagccg
    24841 ccgtgaaaag aagaaggttt tttcgacgac aaccagcggc gccgttgatg caaagcggct
    24901 catcgcatgc agtctgtgca caaactccgt tgtgccgccc gatgtggttt tttacgttat
    24961 tttccttttt gcggttcgaa ctgatttttc gccgaccaga aagacggcag atgacgtatt
    25021 aagtacaaaa ggctattgcg atgctgccag atgcgattgc ggaatttttt acgccatttt
    25081 ttatcagaat aaaaacgctt cacgtattcg ttatagagct gatccagccg ttccttcgac
    25141 gcaatgcccc tgggtacaaa aacaaaattc agacagttca tcagccgcca gtcttcgtga
    25201 aaagtcccct cctctctgat ggccgtccac agcggggcgc cgggaaaggg cgtaaacttg
    25261 gccatgttca tatcgtcgag gtccagcgac agaataaaat ccgatgtgcg tttgacggat
    25321 gcctccgttt cgccgggcag ccccatcata aaaagaccct tggcgcgcag gccggctttt
    25381 tgaattcgtt ttactgtgtc gcgcacgtca tccaggttga tgcccgattt gtgccgggca
    25441 agcatggcgg ggtcggccga ttcgattccc agcgacacca tcaggcatcc ggcgtctttg
    25501 agcatgtcaa gcaaatcatc atccgtgtaa ccgatgcgta cggcgcagtt gaaatgcaga
    25561 cccagaggac ggcggctgag tttttcgcag agcttgacaa tccgcggacg gttggcggtg
    25621 aataaatcgt cgtaaatgtt gatgtgccgt acaccgaatg tctcccgcag atatttcatg
    25681 tggtcgtaaa tgtagccggc ggaattgtag cggaagcctt ttttaaaaac ggagcggtcg
    25741 cagtaggagc actgatacag acaaccccgc gacgtaatca tggtcgcgcc cggcgtatgg
    25801 atgtagctga acaggggcag gtggtaatcg cgggggaaac ctttgagctg ttcataagcg
    25861 ggaaagggca gactgtccag atcggatatc ttcgggcgca catcgttgac cgtgatttcg
    25921 tcctggttgc gccagattaa gccccggatg tcggcggcgg gaatctcgtc cgcgagttcg
    25981 gcaatggtct cctcgccttc tccggcaatg agaaaatcaa atgccgggta agcgttcaga
    26041 agttttcctt ccaaagcgga tacgtgaacg ccgccgcaga cggtggcgat ggctgaagaa
    26101 tgatttttga tgagccgggc cagatcagcg gcatcgggga atgaggatgt tgtggccgaa
    26161 aagccaacca tctgcggctg ataggccaga atggcgccgg cctgtttttg cagatcgagc
    26221 ggcgctcccg gtcccagaca gtcgtagacg aaaacctcat gtcctttttg tttcagatag
    26281 gcggcgatgg acagcacgcc ctgcggcacc atccgattgg cgatatcggt gatatcgcgt
    26341 ttgccgggaa accagttgaa tccccgggga tggacaagaa caatgcgttt tttctgccgg
    26401 gaagaaacca tcatgatttt gttgagcctg aaacaaaagc atgcctgcct gaggcactgc
    26461 agttataatg cacaaaaacg gaattggttt tttcgcgcag cgttcgcgcg atgccgaaag
    26521 cggatgccgt ggaatgtatt ccgcagcagg gcagataatc ttccgtttcc cagccgccgt
    26581 ctttccagcc gttcaggtcc gtttgcctga tgcggaatcc gggaagaaca atccggcctt
    26641 ttcctcttgc atcacaaaga tctgacagaa tcgtttgctt atcgaaacgt tggtccggcg
    26701 cgccgacagt gtcgtaaaaa tccacggcta gaatatgccc tatattttgt tcttcattct
    26761 tgcccagcag gagccaggaa ctactttcgc ccatcggcag tctgcgggca ttaccgcagc
    26821 tgacattcac ccgttcgcgg tattcttcag ccgtgggtgt aaagatatcg cagccgccga
    26881 ccagcgcgat gtctatccgg ccgtgttcca gataggtttg tgccagccag agcgccgact
    26941 cgaatgataa ttcttcctgt gagacggtca gaacggttcc ggtaacctcg caaatccgcg
    27001 ccatcacgaa agcggcggca ttgctgaccg aattggcaaa ctggttggga ctgggaaacc
    27061 cgccgccatg tttgaacacc tgtgtgatga aaggcaggat ttcgtcaaca ttgcccagac
    27121 cggtggccag aaagattccc gttttcttcc ccttcagata atcgggtgag acctgctgga
    27181 ggcaggccag gccgccgcag gaaaccatct tgatgaatct tcccgcgcgg cgcatcggct
    27241 gtcccataac ctcgttgacc agagcatcgg cattcaaggg caattcgttc tcttcccaga
    27301 gcgcatcgcg gttgggcatc agcgggggac cgatataact tgccgcgatc accggtatgg
    27361 gtgaggccgg ggccgtgtgt ttggtcgtcg tttctgtcat atgcattccc ttatccgata
    27421 gggttgtatc tttcaggacc tgttatgatg agccggcacg agcggaggag ttttcggtgg
    27481 agttcctgat aatcaaataa cttgtgcatg ttccgccgaa gccgaaaaac gtaaacaggt
    27541 aagcgccgtc gcgggcgtca atattttttt gcgaaggggt aaagccgatt tccggatcca
    27601 tttcactgaa accgaaactc cgcgggatga ttccctcgtc caggcaggaa agccagagag
    27661 ccgtttccgc cgtgccggcc gcgcccagcg tgtggccgat ggcgccctta agggaaacca
    27721 ccggaggaag tttttccccg aagagtctga ccagtccccg gccttcggaa agatcattgt
    27781 cttttgtacc tgtgccgtgc gctttgatgg cgatgatatc gtcggccgat agcctggccg
    27841 agtccagggt atggcgcacg acctcgctta cggtcacgcc gtcgatgccc gccgatgtca
    27901 tacttttgat atcgttgtag agatacccgc cgctgaatgt atagcgggag gataaatttt
    27961 gaccggaatc cagaatgacc gcggacgcgg cttcgccgat ctgcatgccg gcgcgggtgg
    28021 cgcagaaagg ccggcatcgc tcgtaatcaa agagcagcag gctggaaaaa ccgtggagcg
    28081 tcatattcag aagcggttca aaaccgatga cgatggcccg gcggattttc ctgctctgaa
    28141 gaagatcggc ggctactacc agcgcatgcg aactggaaac gcaggcgatg ctgaacgtca
    28201 gcgccattcc ccggataccg tattttttgg tgatgaaatc ggccacttcc ccggcaccgc
    28261 ggttgcggca ggaaatcggc ggcgtttcgc cgtccggatt tttgcggacg gcttcgataa
    28321 attcatgttc gttgcggata aaaaggccgc cggtcgtgcc gatcaggacg ctggattcgg
    28381 cgatttcatc cggggatagg ccggcgcgct caaacgcctc atcgatcgcg ccttccagca
    28441 tggccatggc tttttccaga gggttgcccg gatgggacac ttcgaaaaca ttgaagtcgc
    28501 ggttaatcag atgggaagac cgccccggtt ttgccggtgg ggcctgagcc tcaatcaggt
    28561 tgcgtgccgc atcggtggca ttccatccca gggtggttac cgcgccccag ccgcggacat
    28621 aaatatcgcc gctgttcatt ttttaaccct tggagcctcg atgataaaat cggccagcgt
    28681 gtcgaaattc tgcattgctt tggcggccgt tgaagcgttt tttaattcga caccgaactg
    28741 aaatctgatt tccatggcga tttccatggc gtcgagactg tccagcagca gcggtccggg
    28801 accgccgatg aagggaacat cggtgggcac gtcttccggc cgcacatccg tgatttcaca
    28861 agcccggatg accagctcct tgagcttgta aatcaactct tctcttgatg acacgtttaa
    28921 gtctttcaat ttctgggtta catccattta actttcctcc ccgctatata aaaacaaacc
    28981 aacgcgaata ccgttaaaac agcaagcttc ggccatatgg aaccgaaccc ggcgtcgcgc
    29041 gaaaaaatat ccaaataagc ctgatgcgcc cagtacatcg gtgagagcat cgccagtttt
    29101 ttcatgatga gcggcatgac cgcgtgcggg accataatac cgccgaagac ggccatcagt
    29161 accgctccgg tggccgccag cgccgaagat tgttccggcg cccgtgccag cgccgcgacc
    29221 atgacgccga acccggtaga tgcggccgcg acgaccagcg tgaccggtat cacatgccag
    29281 gggtgctctc ccagcgtaaa agggaatgca atgatcagcg gcataatata aataccgacg
    29341 atcagcatta aagcaaattg catcagatta atcaaataat aaggaatcag cttgccgaga
    29401 atgacaatag cggggctgac cggataggta aaaatccgct gcagcgtgcc gtcgtttttt
    29461 tcccgcagaa aaccgatcga catgggaatc gctatgaaaa acatcgcaaa aatcgaccag
    29521 ccgggaacgc tgttttgcag cgggccgggc aaggtgcggt tcgtttttgt cttttcaacg
    29581 ataaaatcgg ccgacatccc ttcgatgtcg tcgatgccca tgaccacctc cacggtcagg
    29641 cccgtgacca gaacacgcac ggcggatttg tatccggcgt cgagaacggg atcgaactcc
    29701 atttccaccg gtttcaatcc ttcatcaaat cccttcggta tgaaaatcac cgcatgcgcc
    29761 cggctggttt cgaaaacctt gtcgttgtcg aatcccggcg gtctctggac gcgttgaatc
    29821 attttgttgg cgctgatccg ttgttcaagc aaattcgccg tcggctgatg cgattcgttt
    29881 tcaataatca gcagaatctg ccgtcccgtc aatttatcca tataaacgcc ctgcagcgcc
    29941 agcgatagaa aaaaaatcag cgccatgggc atgatgaata aaagcggaat ggtctggcgt
    30001 tcgcgcagca aaatgatcat ttccttgcgg atcaacgctg tcagtttccg gaacgtcact
    30061 ttcgtttccc ctctttaatc ctcatcgcgc aatctgtgtc cggtttgtgc cagaaaaaaa
    30121 gtttccagat ggtcgatttc gttcatggcg ccggaaaaaa tcaccgctcc ccggtccagc
    30181 atggtcacct ttgaacacat ccgctgcgct tcgtccatga tatgcgtgca cagaacgatc
    30241 gcaattccgg aggcattcat ttttttcaga gtttcataaa tatggttgcg gctctgcggg
    30301 tccacgccaa cggtcggttc atccaggagc agcaggcgcg gtgcgtggag aagggcgacg
    30361 gccagattca gacggcgcag catcccgccg gaataggttg acgcccgttt ctgcgcgtaa
    30421 ggcaaaaggc cggtttcttc cagaaccgcc cgggaccgtt cttgcagcag ctcgcgcgac
    30481 aacccggcca tgacgccgaa aacgcgcatg ttttccatgg cggtcagtcc cgcatatacg
    30541 gagatgggct ggggcgcgta accgacggaa ctcagcgatt gtgcatgatc gttgcgggtg
    30601 ccgtaaatcg atatgctgcc ctgaaatcca cgcagctgcc cggtgatcag gcgcatcaac
    30661 gtggtctttc ccgctccatt aggtcccaaa agtgcgtgca tctggccgct gtccaccgac
    30721 atggaaaaat cgcgcagcgc atattcagcc ccggcggttt tgatttttcc ctctttgggg
    30781 ttataggaaa aagtgacttg cgcaatgtcc agagctttca tgatttcagt tttccggcaa
    30841 tctcatgcag atttttaaaa agattttctt cacggccggc gcagatcagc attaatccgc
    30901 ccgctttggc aaacgtctca tcctgttttc ctcttttttt gatgatgcgc gcggcggtga
    30961 cggcaaactc gcgccacgta tcgccgatgg ccgtcatgtc ctgcgataga tcgttgagtt
    31021 ccgccgagcc gaaaaggtcc gcggcttcct gcagaaaggc tgcgtacata tagcggaatc
    31081 cagcgccgcc cgtgccgact tcctcctgca tgcggacgat catgcccaac tgccggcagg
    31141 cttcttcgaa cccctgcgtc ttctcgaatt tgatgattcg tttcgccagg aagcggatgc
    31201 cgcggatgcc gaaaatcggt accggaattc tgagcatcat gttgcaggta tccagcatgc
    31261 ccttgatgca ggcgccgcgt aagtcgggat gaagattaac cgagaccggg taatacatga
    31321 acccgtgcgg ttccagcggt ccgcgggcaa accgcgcccg gatcaggtct tcggtccggc
    31381 aatccaccgg tttatccaaa acgggatcgc tcacgcggaa gccgttttca atttcgtgaa
    31441 gaataacgat gttgtgcccg ttgaattgaa accggaaacg atcgggaaaa taagaaagcc
    31501 agtaaatatt ggtggtcagt ccgacaatct ggccgctgcg cagcacacgg agcagttcgt
    31561 ccatgccttt ttttgcgttg gtgtattttt ttgtgacaaa ttttccatcg agccttttgg
    31621 cggctttgcg aaacaccgca cccggttgcg cccgatatgt gctgatcggc aggccgacaa
    31681 atttaacaaa cggcagatgg ccgaaaaaga tgccgctgcc gatgccgaaa atcatcggct
    31741 cgctgatttc caatccctta tcccggaata gcgccgccgt cacgccggtt tcacagtggg
    31801 cggcatgatg gtgaacaaac ggtttcccgc cggatgtcag ttctgtatga gtcaataata
    31861 agtcctttaa tgagtcccaa gttttcatga aacgccaatt ccgaaagcga ataacctaaa
    31921 ggacgctata ggcgcaatgg aattggtaat tgtaatgagt ttcaaatttc aaaagcaaag
    31981 cgtattgaaa tttgccagcc gaattatcca atccccatga atcgggattg gaaattatca
    32041 cactaacgac gcttagaggc accgtgccgc atgctacagg atttattccc gcgattcctc
    32101 aggcacggtt tttaattctt ccatcgtgat tgcaagagct ttcgcatagc gttccaatac
    32161 cggatttttc agcctggaaa aaatttccgg ccgcagatgc cgcttgacgc gaaacacggc
    32221 aatgccggcg gtcttgccca ggatatccgg tatcatctgc cgccgttcca tgtgataggc
    32281 gagcgggctt ttttctccgg ccagtacggc ttttctggtt ttctccagat ttacagccag
    32341 ttcctttaca gccagaccat tgacaagctc ctctgcttcc catccgttac tggacacaat
    32401 gacatacctg cctttttcat cgaccgcata gcaggcgcgc ttcacgcctt cataatattc
    32461 aaccgtgtta tcctgcggca cttcgttgac tttcacgaat aaatccttct tttcatttac
    32521 ttatgtagtt tcttatacta aatttaaaaa agcgaaaaga gatttttaat gagacccgtt
    32581 gataatgagc cccaagcttc agatacagag taaactgaag ctggctggac gaattatacc
    32641 ggcgcgtagc gcagtggtag aacgagcttt gtgaagtttg cccctgccac cctaagggtg
    32701 gcgggataat ttgctctaag attttccggg cactacgttt cggaaaatca aggctcatga
    32761 aattattccc gaagcgaagc ggagtgggat ttttccaaaa acggctcgaa atgatgccac
    32821 tcgaagggat gctggcgggc aaattcagcg agatcatcgg cataggcctg caccgaattc
    32881 tgcacggctt ttttccggtc agcgcgtgcg gcggcagtaa cctttcgccc aggcgaaatc
    32941 cggatatgat actttccaac ggcttcctga taaacaaaaa acgtcatcag cggcgctccg
    33001 gtcatcaatg caaacagatg cggcgtatcc ggcaggcgaa cctcgtgccc gagaaaattc
    33061 accgcgatat gactctgatc gccccataaa cggtcgccgg ccatggagac gatgccgcct
    33121 tcccgcaaaa aattgatgcc gtcgatcagg gcgaacggtg atttctcgtc ttcggatgtt
    33181 gccacgattt ttatgccgct tttggccatg gcttctttct gaatacgctc aatctgttct
    33241 ttgtgcttcg cgcccagata cagcatgatc ggcaggcctc ttttgttgag cgtctgcgcg
    33301 gccagctccc agttgccgat atgcgacatt agcaggatcg cacccgtttt gttctgcacg
    33361 gccgcatcaa gatgttccca tccttctttg atatactcga tatcgttttc gtcaaagcgg
    33421 atgaaacggt ggatatagac gtcggtgaat ttatgatact gttgccatgc gcaccagaga
    33481 tggtagaaaa agcccttccc cggaaataag gtgcgataaa actcgaaact gttagccaca
    33541 cgcaccggaa aaagaaaaaa ataacccgtg gcgataaacc aggaaatgat tcggaaaaac
    33601 cacaacccca gcctgtgggt gaaaaagagc aatagcttat aaggtagttt cttcataact
    33661 caatatcttg tcatcttcga cctgatcggg gacccggtat ccagtccttt ttcttccaac
    33721 gtgagccagg taaagatttc gacccttatt attttttaat gatggaagcc gatccagttt
    33781 ccatcccatc ttgcattgag gaccagggga tcgtcggatt tataccagta ctcttcgatg
    33841 aagtggtcga atgcaacctt ttgaatgaaa catatatcgg aattactgct gtccaccgct
    33901 acgcaatcac attgatttcc tttgtgattt tgatcgtttg gatccataaa atacaccgcc
    33961 ttctcggcat catcatatcc gatcaccaga acataatgac cggaagaagg aatgcccagc
    34021 accggcgatc tccaaaggtg caccagcact ggagagccgc tattcaaaaa cggaaccatt
    34081 cgattaagaa aaatattcct tttttgttcg gaaatgctgt cctctgttcc ggtcagatca
    34141 ttgctgtaat tttctatcat cacctcactg tacaacgccc ggctacctga ttcatccaga
    34201 aggttttctg ccgcccgagc taaggaagtc aggtggatgc ctgccaggtt gttgaatgag
    34261 ttgtccatgt attgaatata tgttgctatt tgcgatgttg ctgacagttc cgtcggataa
    34321 gcaattctat ctgtgatttc cgccggacaa ttgtcgtcat caattccggt tttgatattt
    34381 ttcagatgat cgccgtaata tttcatggtc atgtaaaaac atgttgccgc gcataatccc
    34441 gcttcctgcg agacgtagta gtatgacgga ttgttccgga tcgtctcata gggatgcggt
    34501 ccggcaatgt tgtccgcgac aacagccaga gaaagatcct gtgtgctgga gcctgcggcg
    34561 tcttcgaccg tcacggtaaa ttgaaatacc cctggagctg cccatgtggt gccggcgatt
    34621 ctgccggaag aagacaaagt gatgcctgag ggcaatgacc ctttggtgat ggagaacatt
    34681 ttcgtgccgt tgccgccggt tacgctgatc tgctggctgt aagccgaagc aatgacgcag
    34741 gagggtaatg aggttgtggc aattctgggt gaagactcag aatcggagcc ggacgaaccg
    34801 gaacagccgc tgaacacaac actcatcaac aataagacca ccccacaaca tacgctcaaa
    34861 ctttttttca ttttttatcc tgtcttctga tctctttctg taaactttta cggtaatgca
    34921 agaaatgata tatgacattg ttgtttattc atagtctctc ttgctttggc tcggtcatgc
    34981 agagggtttg ctgcgtggct aacccttctt tcccataagc aaagcctcct tgtttcttgt
    35041 ctcatctgac cgcgtaaata atgaagacca gtcctatcaa aatgcggtag tatccaaaat
    35101 atttgaatcc atagttttca acaatcttta aaaatatctt aaccgcaaga taagcaaaaa
    35161 taaaagcaac cgcaaaaccg atgactaaca gcgtctgctc gtaagacgaa aaggcggcat
    35221 cggttttgat caaatcgtac cccgttgctg cacacatcgt gggaaccgcc agcagaaagg
    35281 aaaactcggt ggcctgcttc ttgtttaatc cgttgaaaac gccgccaata atggttgccg
    35341 cggcgcgcga tacaccggga atcatggaaa cacattgtgc caatccaatc aatatggcgt
    35401 gcttgtagga gatgtcctcc aactccatat attgtgtttt actgttcacc acgcgtttgt
    35461 ctacgaggat gagaatgacg ccgccgacaa tgagcgctgt agaaacgatc aatgggttga
    35521 acagatgcat tttaatggtt ttataggcca gaaaaccaat caccgccgtt ggaataaaag
    35581 caattccgag cttgatgtat atttttatgc ctttcagaaa acgcttatag taaagcatga
    35641 tgatcgctcc gatcgctccg atttgaatgg caatttcaaa gctctttacg aaagcattgt
    35701 cgtgaatatt catcaaggat gaggctaaaa tcatgtgccc ggtggaggaa accggcagaa
    35761 actcggtaat cccctctatc gctgccagaa tgattgtctg aataatattc attttccccc
    35821 tcaattttcg ttacgacaca tagatggaat cgtgccgtat tattttttat accggtaaaa
    35881 atctactttt attttcgtat tttcataaag gattttatat ttacgtgttt tcctaaaccg
    35941 tttttctgca tttttcaaac atttgcggga atttcctgcc ggcctaatat tttttacccg
    36001 caaaccattt ttcttcaata ctccgggtat cggaagcgaa gacctggacg gcaaagccgg
    36061 cctgtttcat gaaatcggca atttccgttt ctgatcgaaa ccggttgggc atgccggtca
    36121 tttttaatcg actggtttcg atccagcgtt tccacggatt gggtttatcc gacggaatgg
    36181 ttgtgcggat aagcaatgtc ccgtctgcag tcagtttatc gtaaatccgc tgaaaaacaa
    36241 tttttacttc atcatcggaa atcaggtgca gcatgtcgag catgagaacg tgatccgcct
    36301 cgccttcgac atgcggtaaa tccggcgccc gtcctgcttc caccctgcca cggctgccga
    36361 tgacgcgcga ggcgatcaaa acccgctcaa catcgggttc aataccgaac acccgggcat
    36421 ccggataaat ttccagaagc caggtggcgg gcacgccaaa gccacagccg atatctataa
    36481 tcctacgggg attttttaca tacttatgca gttccctgaa catcggatcc atcattattt
    36541 tcatgcgagc aaagaccctc ggatacccgg gtagatgccg gtagcgcaaa acggtccgcc
    36601 gcagatggtc gcgggaaccg attaccactt tgtccgcggg gtatgcaatc ggcgcaaata
    36661 ttttgtccat cagagacggc aggataaaaa agacgccgat cagggagtaa atgattccga
    36721 gaaaggagac aatgccgata ctgcgcaaaa gcgcgtggtc ggccagtgcc agcacgccga
    36781 agccgattaa ggtggtcagc gccgccagaa atatggccag tttgatggtg tgcatggcca
    36841 gacttttttc atcgacgtta cgctggtaat gacaaatgta atagacggaa taatcgtcgc
    36901 ccatgcccat gatgatgatc cagagcataa ttccgggaat atccagcgga tggccgatga
    36961 ttttcatcgt gccgagcgtg gcaaaaagcg cgaagacaat cggcgccatt gctgcaagcg
    37021 tcagctgcca gtccagaaaa tatagaaaag taatcaacac caggccgatg ctgatgatga
    37081 tggcgatttc caggaaaaga tctttcagaa aatcgcccag ccgcttgttg aacaagtcag
    37141 cgtcgaatat tttagccaga ccgcttcggg aaagccgctc aaagaagtct tccgcgttgt
    37201 aatttttacc ggaggcgagc aaactgagtt gcgtataacc cgcggaactt ttggcaatcc
    37261 ccagcatatc gaaatatttt tcaggtatat cgaacgagcc gatggttgtc cgat
//
LOCUS       DAOSME010000020        37242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1493_length_37242_cov_22.380832, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000020 DAOSME010000000
VERSION     DAOSME010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 37242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37242
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_37242_cov_22.380832"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..875
                     /locus_tag="PLG98_04685"
     CDS             147..875
                     /locus_tag="PLG98_04685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOE32582.1"
                     /translation="MPEASIIIRTKNEEKWIGECLNRLFNQTYRDFDVVVVDSGSTDR
                     TLEIIGKYDVHLFQIPPEQFSYPNALNFGCSRSSAAKYFVFLSGHSLTVSQNWLKDGI
                     ENFKTADNIAGIYGDVWALPDGSFWEKIIFNRCQIILKNYFRRKRVLKKKQMGILAFT
                     NAIIRRDLWERYPFNENYGLGGEDMEWAGYWLQKGFVVIRDRNVCVYHSHGLGLKQLF
                     QQRKNWISLAEPQPFRVTEFRNGR"
     gene            904..2571
                     /locus_tag="PLG98_04690"
     CDS             904..2571
                     /locus_tag="PLG98_04690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE32583.1"
                     /translation="MRRKTNQAFLFFSHLSSEIIIREFNHIRRAVCDMGDAFFLYDTT
                     GKDVPEKIKKLSPYLYSDQCLCDLGYPVIGKKMIPGHAHFPLFKFYLDHPDFDYYWFI
                     EYDVRFTGPWRSFFESFLVHDADFLTSHIRSYAEERSWPWWALKHPRQHIPLDKRLRS
                     FNPLYRISRAALSFLHDAFRNGWCGHHEVALPTLLHQKGFTVREISGRGKYTIPGMEN
                     RFYAMSAPNVKGDLSGTSMKYRPSSWRCGREENKLYHPVKPLKKAVHENILYYGGFLS
                     HALSDVDASGILLRLKIRHRRILDFVKYRLPKKESFRKPRVGIIITSFNQIDYTDRAL
                     ESFYATINKNIDYELWLLDDSSSEDIEGIYHKYKDRGVKFFKNKTNNGLTSLWNKGIE
                     LNKDKDYLILCNNDVVFSNYWADHLIGSLKASPGFAVAVPVTNAPGHVPAQHIANFVD
                     DYVPSDNQDEINLICTKVKNKNFRKTAKGNGFCFAARASLFRQMSTQGGPFNEKFPLY
                     GSEEDFFRRVKPKTMIVPESFVFHYKHVSVESDYFPDQKFRRKTKSL"
     gene            3035..3142
                     /locus_tag="PLG98_04695"
                     /pseudo
     CDS             3035..3142
                     /locus_tag="PLG98_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(3555..5798)
                     /locus_tag="PLG98_04700"
     CDS             complement(3555..5798)
                     /locus_tag="PLG98_04700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOE32584.1"
                     /translation="MRKYILPLLALILLFRIGYTFFDVGRGLSEATAEGPSIFYGRST
                     VIKKGVHLENMRLPDRLRRLSYKKVAGPPYAAGTFSEEQSRWRIFLKNSPDRNPSEDN
                     GPVEIVLRDGRVDAIISSTGKTLTSIQLEPEEIVRIIDEKMPSRPAVGLSAISPFLQN
                     AVIASEDARFYSHMGIDLPSVVKAFFSDPAKRNFPQSSSTITQQLARNFFLSPEKTFA
                     RKLRESELAFVLELKYTKKQVLEMYLNRIYWGQDGSRSIYGAGSAAGFYFSKSAAHLS
                     LEEASLLAGILHSPNRYRNFKAAKERRNAVLSRMKDLKMISDGEFQRACKAPVRVKLS
                     KAPAQTSYFIDFIQRITKDDLGSEKLYHRGYRYYTTMDPVVQAIADEALAEGIREIER
                     RAFPAGEKLQAALVAVDPATGEMIAMVGGRSYRETQFNRAVDANRQSGSAFKPFVLLA
                     ALSLSLEQGKDITLSSTVSGKPLSINTPEGMWRPTNFENKHYDRITIRKMIEDSVNTA
                     TTRLAGEIGFREVLKAARRAGITSPLSTVPSMALGSFEVTPRELAYAYTTIASGGIRF
                     DPFALYSVKMANGRTIISKTVRREKAFDPRIIYLAGYAMQGVLERGTAAEAKALGIYF
                     PAAGKTGTTNGNRDSWFVGYTPDIVCAVWVGYDSGADTGLTGARGALHIWSRFIRSFY
                     GRSAPANIVPPAGIETAIIDPQSGYLATTSCPQKFKEAYLKGTAPKKACPDHPVKPVM
                     KRIYRML"
     gene            complement(5945..14671)
                     /locus_tag="PLG98_04705"
     CDS             complement(5945..14671)
                     /locus_tag="PLG98_04705"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOE32585.1"
                     /translation="MEKIIFFKYFHRLKSRLAGKHQNEKLTEDELLRSELFSAKQMRQ
                     HGKTIAGAHKLNEGLTSNQLLERLTENEKVLFQAREKLTKAVLENRRVTPAEEWFLDN
                     FYLIEEQIRTARRHLPKVYSRELPHLLNGPSANLPRVYDIALEMISHGDGRVDSKTLS
                     LFVSSYQSVTVLKIGELWAIPIMLRLALIENIRRIASRISKDRTNRDLADYWARKMIA
                     TVHKDPKNLFLEIADMVRSEPPIAGPFVAEMARQLQGQGPALALPLTWIEQQLSEYGQ
                     TIKNFVQMESQQQAADQVSMSNSIVSLRFLGTMNWREFVETMSFVDQVLREDPADIYV
                     KMDFATRDRYRHVIENIAKKSSLSETEVARMAILLARKTSNQKGGQDRTAHVGFYLID
                     NGLPELERLTKMRFSLFESVARFSRRFPLLIYVGSIFLLTFTAAFLLLAMAYADGLTG
                     WLLAVIWLVALLSTSHLSIALINWVSTLLITPAPLPRMDFSRGIPPEYRTLVAIPTML
                     LNDKNVGKLIESLEVRFLANRDENLRFALLTDFCDAKTEKMSDDGQLLRLVQKGIESL
                     NEKYADDRGDIFFLFHRSRRWNPKENIWMGYERKRGKLAELNAFLRGITHGPEGGLFS
                     LIVGDTQELRNVKYVITLDTDTKLPRDAAWQFVGTMAHPLNRAQYDEQKRRVVQGYGI
                     LQPRVSVSLPDISRSLYSIMHGSSSGIDPYTRAVSDVYQDVFGEGSFIGKGIYDIDAF
                     EASLNGRFPENYILSHDLIEGCYARSGLISDVQLYEDYPSAYKADVSRRHRWIRGDWQ
                     IARWIMSRVPSADGERLGNPLSVLSQWKIFDNLRRSLVPASLTLMLILGWLLSSSPGL
                     WTLAVLSIIVFPALVMSLMSVFQKQQNVILTHHLSSVFQKTGNNFLQVLFSFICLPYE
                     AYFNTDALLRTLWRMLISHKRLLEWTPSENTNNQRDDLAGYTRTMWFAPSLAAAVFAA
                     LLTAVPTGVPSAAFCLILWLLSPAVAWWFSQPSRLREARLSSAQINFLRKASRKTWAF
                     FEEFAGPEDNWLPPDNYQEHPVEVVAHRTSPTNIGLSLLANLTAYDSGYITVLKFIER
                     SSNTLRTMTSLERYKGHFYNWYDTQSLDPLLPLYVSSVDSGNLVGYLFILKSGLSSLP
                     DKKIIDPQILDGLKDTLGILNDSTGKSVSANLSQISKTLDDATASPPASLTEMKIVLE
                     KIISLAEETASCMRPESDASNAAGHWLKAFLQQATAALDEMLFLTPWLFDPQFSDLIR
                     EVPELDDIMTLRELTSLYEHVESKTEAMKRFESTAIDLSRISDLKNLIAESGNRAVNL
                     MQNIDLLARQCTEFTDIEYDFLFDKSCNLLSIGYNVTNSRRDTSFYDLLAAEARFSNF
                     VGIAQGKLPQESWFALGRLLTMAPGKKPVLVSWSGSMFEYLMPLLIMPTYEGSLLDQT
                     YKAAVAAHIEYGKKRSVPWGISESGYYAFDSHLNYQYRAFGVPGLGLKRGLAEDLVVA
                     PYASALALMVEPEKACLNLEKLASREFQGKFGFYEAIDFTPSRLTRGQSNAVIRSYMA
                     HHQGMTLLALTYLLLDRPMQKRFEAEPLFQSALLLLQEMVPKAVAFYASPSELISAQK
                     EPEISENPVRVFNTPDTTVPEIQLLSNGRYHIMLTNAGGGYSRWKNLDVTRFREDVTC
                     DSSGIFCYLSDVKTGDIWSTTYQPTLKKPRNYEAIFSDGRVEFRRRDYEFDVHTEIVV
                     SPEDDIELRRVTITNQSRLQKTIDITSYAEVVMTHSAADKLHPAFSNLFVQTEIIEQR
                     RAIVCTRRPRSEQEINPWMLHSMAVHGQDIEQISYETDRSRFIGRGNTLADPQAMGPP
                     SDLFGTLSDSQGSVLDPIVAIRSRITLGPEQSAVVDMVFGIGQTRDEAITLVDKYQDR
                     RIADRVFEMAWTQSQVLLRQINATEADAQLYSHIASSLIFPNDSLRAGAGVISENRRG
                     QSGLWGYAISGDLPIVLLRVSDQSHIELVRQLIQAHAYWRLKGLAVDLVIWNEEHAGY
                     RQLLHDQIMGLVSSGFVADLKDQPGGIFVRSTDQMPEEDRILIQTVAHVILSGNRGKL
                     ANQVKQRGGLKTAAPRMIPNRTHREPPVPKAAPPRSDLVFFNGLGGFDPSFREYVITS
                     SKSQITPAPWINVLANPTFGTVVSESGMSYTWADNAHEFRLTPWHNDPVSDRSGEAFY
                     IRDDERGHFWSPMPLPHRGLEPYTTRHGFGYSVFEHTERCIQSEVWIYTALDASVKFI
                     VLKIKNLTERTRRISITGYIEWVLGGLREKNAMHIITEADHQSGAIFARNSYNMEFPN
                     RICFFNSSASIRTITGNRMEFLGRNGTLADPAVMKRQYLSGKVGAGLDPCAAIQVPLE
                     MLEGEEKEVVFTLGMGRNVTEARKLAQFFSDPATAADALQAVQNYWREKLDAIQVQTP
                     DPSVNVLTNGWLLYQTLSCRLWARTGYYQSGGAFGFRDQLQDVMALIYTEPNFAREQL
                     LLAASRQFEEGDVQHWWHPPLGRGVRTQCSDDYLWLPLTTCCYISRTGDQGILDEIIP
                     FINGRPLNREEDSYYDLPVRSPEKANLYEHCLRAIKRGLRFGEHGLPLMGSGDWNDGM
                     NMVGIQGKGESVWLGFFLYDILTQFAKIASRRGDTSFEKCCRKEALQLRLNIEQHGWD
                     GLWYRRAFFDNGSMLGSARSEECRIDSIAQSWSVLSGAGNGHHSRLAMESLSQHLIRR
                     DIGIVQLLHPPFDKSGLNPGYIKGYVPGVRENGGQYTHGAVWAAMAFAALHDNNRAWE
                     ILTMINPVNHANSPESIAIYKAEPYVVAADVYAFSPHTGRGGWTWYTGAAGWMYRLIT
                     ESLLGFKKEDDTLYIEPCLPAEWGGFSMHYRYKKTFYRIVVTVTPERKKEKTVLLDGI
                     RQEEKAVLLTNDGIDHQVDVIL"
     gene            complement(14789..15643)
                     /gene="rpoH"
                     /locus_tag="PLG98_04710"
     CDS             complement(14789..15643)
                     /gene="rpoH"
                     /locus_tag="PLG98_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HOE32586.1"
                     /translation="MTALEFPVVTGTLNLYIAEINRFPLLTAEEEFKLAVRLKKYNDV
                     SAAEKLIVSNLRFVVKIAHEYRNYGFKLSDLIQEGNIGLIHAVKKFDPYKGYRLISYA
                     VWWIRAYIQNYLIRSWSIVKIGTTQAQRKLFFKLNQTKKELENLSRKNPEFSEIATSL
                     GVKDSEVEEMDLRMGARDVSLHEIINDESSNSHIDFLAYEGDDQEISLIKKEEKSLVQ
                     RNIAGALANLNERESYIILNRVMSDNPKTLQEIGEKYNISRERARQIEKQALKKMQLA
                     LPYFKPNK"
     gene            15857..16729
                     /locus_tag="PLG98_04715"
     CDS             15857..16729
                     /locus_tag="PLG98_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOE32587.1"
                     /translation="MKTINKTMIDHAVKIAREIKANALLVCADVVSDVSLIMDEIEKD
                     IAFIMVSREDESLSEEMKKDAKLLYIPNVNLTRVGIIKIAIAKGIILGALKRGDKVVC
                     LSGVPKFGYADSIFVIDVGKEFEILTSDFINDVLENVRPEVFNAVLNIALELAAQGRE
                     SRKVGTIFVLGDDEKVMQLSRQMIINPFLGYPEDQLNILNPDLEETIKELSAIDGAFI
                     IKENGVLVTAGRHLSAALDSKDFPPGLGSRHIAAAGITSLTRAVAIVISESSGNVTIF
                     KNGKLFVTIEKPVE"
     gene            17081..19672
                     /gene="clpB"
                     /locus_tag="PLG98_04720"
     CDS             17081..19672
                     /gene="clpB"
                     /locus_tag="PLG98_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOE32588.1"
                     /translation="MRFDKFTLKVQEALQEAQTLAGSMGHQSIEPEHLLVCFFRQEDG
                     IAGAIINKLNASSQKIEKELDGYLKKQPSVGGAGAGQVYLGGRLNKIFDKAMTEAAQL
                     KDEYVSAEHVLVCIAEEKEGQAGKILRQAGVTKDNIFKVLKDIRGNQRVTDPNPEGKY
                     QALERYARDFNALARKGSFDPVIGRDDEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPENLKNKRVIGLDIGALVAGAKYRGEFEERLKAVLKEVISAEGEII
                     LFIDEIHTMVGAGASEGSMDASNMLKPALARGELRCVGATTLNEYRKYIEKDAALERR
                     FQPVLVKEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSHRYITDRFLPDKA
                     VDLIDESASRLRIELDSMPSEIDAIDRRIIQLKIELQSLKKESDKTAKERRDKIEKEI
                     ADLQEKMDQMKVHWSGEKDIIKKIQNIKSRIESLKTEEANAQRDGNLEKAAEIRYGKL
                     VALDKELQNEQKKLEDIQKNNQMLKEEVDAEDVAEVVSNWTGIPLSRMMESDVQKLLH
                     MEERLKLRVIGQEEGINAVSAALRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFMFDNEQAMIRIDMSEYMEKHAVARLIGAPPGYVGYDEGGYLTEAVRRKPYSVLLFD
                     EIEKAHPDVFNILLQILDDGRLTDGHGRTVDFKNTVVIMTSNVGSQWIQDPSLDDEEK
                     RNRSMDALRATFKPEFLNRIDDIIMFSALKLEDIYKIVDIQMGLIDKRLKERKMSVEL
                     TEKAKNYIAGQGYSPVYGARPLKRSLQKLILDPLAMEILAGKFAEGDKILIDQSKKGE
                     ITLTKKS"
     gene            19706..20104
                     /locus_tag="PLG98_04725"
     CDS             19706..20104
                     /locus_tag="PLG98_04725"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOE32589.1"
                     /translation="MEDKKQGSGFVIKDKRHFGESAEEQKQEETKTAGAEEKQKGPEP
                     TKQGESDYPPVNFTNFILSLSTSAFFHFGDLAESEDAAPQKNLPAAKQTIDILDMLNE
                     KTKGNLDKNESGLLQGIIYELKMRYVKEKG"
     gene            20106..20945
                     /gene="ispE"
                     /locus_tag="PLG98_04730"
     CDS             20106..20945
                     /gene="ispE"
                     /locus_tag="PLG98_04730"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOE32590.1"
                     /translation="MHWIAPAKINLFLKILRRRADGYHDIFSLMQKISIYDELIFAPL
                     HRGIVLHCPGMNLPENDGNLIVRAAKAIFAYANYSGGIEITLIKKIPMTAGLGGGSSD
                     AATTLLALNKICALKLKKNELMAIGAKIGADVPFFVFGNTALATGIGDKLKHLRNLPP
                     LNLVLIKPPFDISTKMVYESLNLRLTMGKNNYSIPRFLKLGDIVQVLQNDLESVSLKI
                     YPELDEFKKMLLGHGALGALMSGSGPTIFGIFRNGKEAKKALECINKEIPGNYTVFFA
                     KSL"
     gene            21004..21078
                     /locus_tag="PLG98_04735"
     tRNA            21004..21078
                     /locus_tag="PLG98_04735"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21036..21038,aa:Gln,seq:ttg)
     gene            21104..22045
                     /locus_tag="PLG98_04740"
     CDS             21104..22045
                     /locus_tag="PLG98_04740"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOE32591.1"
                     /translation="MLERIRIFSGNANKELAEKICNTLGVSLGKANVTTFSDGETRVE
                     IDENVRGMDVFIIQSTCPPVNVTLMELLIMIDAMKRASVDRITAVVPYYGYARQDRKV
                     ASRAPITAKLVADLITAAGANRVLSMDLHAGQIQGFFNIPVDNLFAKPVLLDYMKKTY
                     MDNTVIVSPDAGGVERARSFGKRLGASLAIIDKRREGPNEAEAMNIIGDVKGKRVIIL
                     DDMIDTAGTVVQAASAMKKAGALEISVCCTHPVLSGPALDRIENSDIKEAIVTDTIPL
                     SDRAKNCGKIKVLSVSGILGEAVRRIYYNDSVSSLFI"
     gene            22068..22754
                     /locus_tag="PLG98_04745"
     CDS             22068..22754
                     /locus_tag="PLG98_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOE32592.1"
                     /translation="MEVTDLAAQVRKEQKKGPSRRLRDKGFVPAVFYGRSAENILLAV
                     KSDELIKLHKDKKDHAFIKLIIDDEGSKKIEKLSLIKELQLQPLTGKLYHVDFYEVDE
                     KRKITMDVALNFIGKAIGVENGGELQHLKREVKVSCLPLDLPDHIDVDVTDLEIGDSV
                     KIRDLKVAEGVTLLDRPDTSVASVAMIKVAKVEEAAKEEGATEGAPAEGEAAATAGAA
                     AAPAEKEKGK"
     gene            22790..23422
                     /gene="pth"
                     /locus_tag="PLG98_04750"
     CDS             22790..23422
                     /gene="pth"
                     /locus_tag="PLG98_04750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOE32593.1"
                     /translation="MLLSVLKKIFFRQVDEREQIMYLMIGLGNPGSRYARTRHNIGFM
                     VLEKIAAQWKISLTQKSFDALWNRGKINNVSVLLAMPQTYMNLSGKSVGRLMAYYKVD
                     IDHVIVIHDDLDLPFGTIRLKKGGGDAGHKGLKSVITALGSADFLRIRMGIGKPADKA
                     GVENYVLQKFNQEEQGVLPESIQLAAEAAADIVISGMQQAMAKYHTKNIK"
     gene            23441..25879
                     /locus_tag="PLG98_04755"
     CDS             23441..25879
                     /locus_tag="PLG98_04755"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOE32594.1"
                     /translation="MFKNKKSIWTFLLSTLMFLLMAGAAFAGEADIQLPDLTQVAFMG
                     GSLQGLTILNVGLIICLIGMAFGIMQYVQTKNLPAHKAMLDVSQTIWETCKTYLFQQG
                     KFLIALWILIAICIVYYFGALQGKGVSDIIIILICSILGILGSYTVAWFGIRINTVAN
                     SRAAFSSLSGNPLNIVNICLRSGMSVGLLLVSIELFFMIIILAYIPKELAGPSFIGFA
                     IGESLGASALRICGGIFTKIADIGSDLMKIVFHLPEDDPKNPGVIADCTGDNAGDSVG
                     PTADGFETYGVTGVALIAFLALALASNPEMGGKLIVWIFAMRILMILTSLASYFVNDV
                     ISKAAFGGKKEFNFEHPLTNLVWITSIVSIAVTFVASYYLLGDLTEPYTSLWWALAII
                     ISCGTIAGALIPEFTKVFTSTSSRHCEETVNASKQGGPSLNILSGLVAGNFSAFWEGL
                     TILILMFIAYMVSKNPAVMALMPPEFTFAAPVFAFGLVAFGFLGMGPVTIAVDSFGPV
                     SDNAQSIYELSLIESRPDAKDVVKKEYGIAPDFELAKHFLESADGAGNTFKATAKPVL
                     IGTAVVGATTMVFGIIILLEHLFGNVVAKLSLVQPEIILGLIMGGAVIYWFTGASIQA
                     VTTGAYQAVVFIKKNINLDKAEASIEDSKEVVKICTQYAQKGMINIFIVIFFMCLALA
                     FFNPYFFIGYLIGIAFFGLFQAIFMANAGGCWDNAKKIVEVDLKMKNTPLHEASVVGD
                     TVGDPFKDTSSVSLNPVIKFTTLFGLLATEIAVTMTNPNLKTGLAIFFFLIAIVFVYR
                     SFYSMRIASEKLGE"
     gene            complement(26110..27726)
                     /locus_tag="PLG98_04760"
     CDS             complement(26110..27726)
                     /locus_tag="PLG98_04760"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOE32595.1"
                     /translation="MINKNNSRYPTYRVLLIIAFLAGFVSLAYEIVATKVLYYFFNES
                     TITTSTVISIFLFGIGLGSFIFSRVEKKISDQRKLILSAQLLIALYAVFIFTDYDLVP
                     SVFNFLYPLFGNDVIMLLFNKLFTSFLYLIFPTVLMGMVFPAIIVMSINAIEQLPQKI
                     GTIYSFDLFGAVLGALFSGFVFIPFFGIKALIFFSVSVNLIIGLLVFFQKNKKFLIML
                     AGFALMLLVFYFIIQPFKSDTAIYSSPDNKEKYIPGENLLFHTGKDTPYFQKVKIKEK
                     IFFSHSPFGELSVFDEVADTEQHRYLYIETRVQCSTVGLKQKEVSEINFANVSIDALD
                     NKNLRVLNIGLGCGFTLHEIVKHPNVKAVDVVEINPVMPEAAKCFSAFTDHVLENPKV
                     KIIFDDGYKYLLGTDKTYDLIIMDIEHPSVIYSSNLYTLEFYKLVKKALHKNGIFTQW
                     SYRPIPEAQVINFNTLKAVFPDVFPKISGVFNDLYYIAGKENIALDEREVTFLNKMLG
                     SNDKRLNTLNNPYFGTEMMLRDLFHVHQDI"
     gene            complement(27873..30170)
                     /locus_tag="PLG98_04765"
     CDS             complement(27873..30170)
                     /locus_tag="PLG98_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32596.1"
                     /translation="MISFNIFKSFKKPTTIKTLGVKNYQIYASLNRLSPGVDNIHLDV
                     HISSQVHNALKKTAFLLMVKHSGAEFFFKEYKKEFCESEKDALRRICNDVLLDGINKA
                     KKASEVQIDLLAQTALAKLFLEEIQLQYNILVEQIEPLVKMYQLSTKHDQNDVFMIKD
                     KLSEIKRHHHHITRLAGKELFELLADVNTRKLRDMRETHFPPRDILPGFFLANPMLHT
                     NNPSDDFFLIEEYVLMGQRSEDTDNYRSIKMILYDLFGQIDLTNEDGRNATEANERKF
                     QTVHGLVDAENCLYDALIMEPDNIGKMLDFYETEEQYEKAKNNKEQESILNKLKERKE
                     SQKNLLNLFYHQFKVAGLLPQIVANFEMKSVYEKYCPPLSPRQVREFLVNFWKRTSMR
                     KNLKRLKSLYGSQFNFGPLLQTVKHIKDCSTKEKKLYLILFLKQFSRYYRDLYNFYLI
                     QDAMNSINLVTEEKIILLSRENHSLFEFLLPDEKITEEKPIANHVIIKADIRGSITIN
                     HTMRKMGLNPASYFSLNFFDPISAVLAEYGASKVFIEGDAIILSLFENEDASEGNYSV
                     ARACGLAIRILRIVHSYNEKNKENNLPELELGIGICYSQDPPTFLFDGDSKIMISQAI
                     NQADRLSSCDKLLRKIFKSQNDIFNLFVFENEAEEIIEAAGEDATYRYNVNGIELNEE
                     GFHKLTREINLQTYIFPSETDENIKLYAGKVPLTNGNYQRIAIREAPIHKLQPSALDA
                     AGKITKKYYEVCTHPDIYDFTDKQY"
     gene            complement(30277..30903)
                     /locus_tag="PLG98_04770"
     CDS             complement(30277..30903)
                     /locus_tag="PLG98_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32597.1"
                     /translation="MKKTVFLILLLLSLMACSKSSENAEDMMNEANLLWDGKQYTEPQ
                     KAVEFLSKAIKLQPDDADTYNQRGVAYKNLGNNQLALEDFNAAIRLQPDFVLAYYNRA
                     TVRNNLGQYQEAIADCGEAIRLKPDFAEAYNTRGINHNKLRSYKMAIEDFNKAIKLEP
                     RNANAYNNRGLAYFMLENNTQGCSDVKKACELGFCKALDLIKSRGYCL"
     gene            complement(30984..32426)
                     /locus_tag="PLG98_04775"
     CDS             complement(30984..32426)
                     /locus_tag="PLG98_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE32598.1"
                     /translation="MSEQKSISEQWGHDFLRTLYHMTSTVRMYQDNNQIVRTSVESFH
                     KILHELTNDGDISLRLNRGRFHLGGEKIPYRRDAAVVVYNMVDFFSKRSIGSLTFLTS
                     SRDVPPEKIMTLARLLNEAVKHDRHVEWLEEQLTKNDFSWVQIQQLQDEDIIAEGENL
                     EEKKLEKARKNYFQAIEAVKEVANKVSHGTAGVRRIRRLAQNIVDLTKQDAALMIGLT
                     TLKDYDDYTYIHSVNVALLAACLGRHIELSDIVLEHLTICGLFHDLGKVDVPKEILFK
                     QGELTNGEWDTVKKHPVMGVKKILMLNANPSLRSKIILGPFEHHLNLDMTGYPQTLFI
                     NNVSLMGKIIHIADVYEAMTSARVYRPKSFMPDEVLKEMWKEAGTSFDTILLKRFIHM
                     MGTYPIGSVVEINNGSFGVVIDYPEETERSLPLIMLLEDDGKGTWQSGKMIYLADQNS
                     LEVKDRLSILRTISPEKFHIHPAEFFLRLK"
     gene            complement(32429..34147)
                     /locus_tag="PLG98_04780"
     CDS             complement(32429..34147)
                     /locus_tag="PLG98_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOE32599.1"
                     /translation="MSKESEKALFIQKSEEMKKLFVQLLLTSKNVSLYPAGHSISLNS
                     IGNFHETLEAYIRKHGDIRFEFERERVTCQEMNVQTGPPEEGSLPFTLFRDGIRWLEF
                     AEGITLEETRELLTIIHQYSVLTKEPEGDIVTAFWEAHFNHILYQADDFISEQATDSL
                     ESMTKFQATPEDQGDGSESADVSETAALHEDAQEPESSEQIGLDPDSFVLTAREQIAL
                     QDMIAREEMLSATEHLNMLLDMLLQYKEEKDFSIILEVISEEFDGSIRRHDFEATLII
                     LDGLRRVLDSGRLRSPYAHDLIEKLYGDISSADRCLEPLKDAWSSLNAQQIEIFKNIF
                     QHLQPQAIHTLTEFLLLGQPSQLEQLLQDTIIALINRDTNPLNQLIGKADEKVAEKLV
                     SVLSKVEGNISLKYLMKLARHPSPSARRLAVKTIGQIEGDYIPRIFEFLNDPDESVRR
                     TILKQMSRTRNEAAEDLLLQYLQNHQFDNEEAWYISECLVTLGRCGSERCIPFLRDIL
                     LQRKRIAGAKKSVCRQGAAFALAALKMPEASRIIEAAGRSFSPSLRRIARKAEKEVVQ
                     KNKKGS"
     gene            complement(34502..36649)
                     /locus_tag="PLG98_04785"
     CDS             complement(34502..36649)
                     /locus_tag="PLG98_04785"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOE32600.1"
                     /translation="MGVADVKVDFNIAIPMTKAENPVGYNCNIYASAFYHTSPYNGTG
                     SIKRPTIFLATGYHRDLIGLMFAVIAFVPHDYNVVVLDMRGTGSGEGVWDPLSPIEQY
                     DVAYMIDKWIPAQPWSDGTVGMVGGSYMGIIQYLAAGLVEQVNGTPTHLKAIAPISAY
                     NDVWKDIVWHGGNFDLEFMAIWILMTDFISILPPDLIVGGVAEPNFNKTDFMNALSIW
                     AQHFKQLTVPLEWFMDSTKGTRNSWYDTKSPMIYWPDKPAGGWNFPDMPPAVGGGVIP
                     KNLPILTATGWYDIFTRGSFMNYEYGLKNHAPGNKAIIVGPWYHIDAAFMFPGVKGMG
                     IEGKEGIFTWDVLRRWMDWRLKGKDDPFLQEFPVLLYVLGENKWRAEKSWPLPSSRLS
                     NQTYYLSKAKSSLIFGDWFGVANYDDNYKLVPSVKTTDYNNVILGIKKPKVNPVLYHS
                     PTALNGAFSRSAQRWLGFSPLTIVSQLSRYTLDINLDSTLFWEDERSDETGVLTFSTE
                     PLASDMEISGPLKLTFWAKTKFTEPLKQAALDNFIDQIKSTFNIGEDKDMILQIADRK
                     DVQWVVEVNDVFPNGRAKNITSGWLSAAFRPYNAAKPTQVDPAYRAFDPFYFQPEKNP
                     NNIAEETVYQYVVELWPTTNVFKKGHRLRVSISASDFPHLFPVLRPSKNTIVIDEAHQ
                     AKLDFKVANKNGEGTLWKWIDGDVGDYLLTHKN"
BASE COUNT         9491 a         8608 c         9288 g         9855 t
ORIGIN      
        1 ctctcacctc aattagttta ttctctatat cactcttttc gaggtgtcca ctatggctat
       61 accagttcac ttcaggattt tccatttatt tatccggaaa gatatagtaa gaagtcggaa
      121 aaatgtgcca cggtgcgaac tcagccatgc ccgaagcatc cattattatc agaacgaaaa
      181 atgaagaaaa atggattggt gaatgtctaa acaggttgtt caatcagacg taccgtgatt
      241 ttgacgttgt tgtcgtcgat tcaggatcga cggatcgaac gctggaaatt attggtaaat
      301 atgatgtgca cctttttcaa atcccaccgg aacagttttc ctatcctaat gccctgaatt
      361 ttggttgttc gaggtcgtct gccgcaaaat attttgtctt tttgagcggc cattcgctga
      421 ccgtttccca gaactggctt aaggatggaa ttgagaattt taaaaccgcc gataacattg
      481 cgggtattta cggcgacgta tgggccttgc cggatggttc gttttgggaa aaaattattt
      541 ttaacaggtg ccagattatt ctgaaaaatt atttcaggag aaaacgtgtc ctgaaaaaaa
      601 agcaaatggg gatattggcc tttaccaacg cgatcatccg gcgtgatctg tgggagcgtt
      661 atccttttaa tgaaaattac ggactcggag gcgaagacat ggaatgggcc ggttattggc
      721 ttcaaaaggg atttgttgtc atcagggaca gaaacgtctg tgtttatcat tctcacggcc
      781 tcgggttgaa acaacttttt cagcaaagaa aaaactggat atcgcttgcg gaacctcagc
      841 cgttccgggt taccgagttt cgcaatgggc gttaaatcgg acctctgaca ggatggctat
      901 ttcatgaggc gcaaaaccaa tcaggctttt ttattttttt cccatctttc atcggaaata
      961 atcattcggg aattcaatca tatccggcgg gcggtatgcg atatgggaga cgcctttttt
     1021 ctctatgata ccacgggaaa agatgtcccg gaaaagatta aaaagctgtc tccgtatttg
     1081 tattccgatc agtgcctgtg cgatcttggt tatcccgtta taggcaagaa gatgatcccg
     1141 ggccatgcgc atttcccttt atttaagttt tatctggatc atcctgattt cgattattac
     1201 tggtttattg aatacgatgt cagatttacc ggtccatggc ggtcgttttt tgaatctttt
     1261 ctggttcatg acgccgattt tctgacctca catatccggt catacgcgga agagcggtcc
     1321 tggccgtggt gggcattaaa acatcccagg caacatatcc ccctggacaa aagacttcgt
     1381 tcgtttaatc ctctgtatcg catatcccgt gcggctttgt cttttttaca tgacgcattc
     1441 agaaacggat ggtgtggaca tcatgaagtg gcgcttccga ccttgttgca tcagaagggt
     1501 tttaccgtcc gggaaatcag cggtcgaggc aaatacacga ttccgggtat ggaaaacagg
     1561 ttttatgcca tgtctgctcc caatgttaaa ggtgatttgt cgggaacatc aatgaaatac
     1621 aggccatcat cctggcggtg cggacgggaa gaaaacaaac tttatcatcc tgtaaaacct
     1681 ttgaagaaag cggttcacga aaatattctc tattacggag gctttctgtc tcacgcgtta
     1741 agtgatgttg atgcgtcggg aatcctttta cggttgaaaa taagacatag aagaatcctg
     1801 gattttgtca aataccggtt gcccaaaaaa gagtctttcc gaaaacccag ggtaggcatt
     1861 attattacca gtttcaatca gatcgattat acggatcggg ccctggaatc cttctacgca
     1921 acaatcaaca aaaacattga ttacgaactg tggctgctgg atgacagctc ttccgaagat
     1981 atagaaggca tttatcataa atataaagat cggggcgtga aattttttaa aaacaaaacg
     2041 aataacggcc tgaccagttt atggaataag ggaattgaac tcaataaaga taaagactat
     2101 ctcatcctgt gcaataatga tgttgtgttt tccaattact gggcggatca cttgattggt
     2161 tcactgaaag catctcccgg ctttgccgtt gccgttcccg tgaccaatgc ccccggacat
     2221 gtgcccgcac aacatattgc caattttgtc gatgactacg tgccctccga taatcaagat
     2281 gagatcaatc tgatttgtac aaaagtaaag aataaaaatt ttcgtaaaac agcaaaggga
     2341 aatggttttt gttttgccgc acgcgcctct ctgtttcgtc agatgtcgac acaagggggg
     2401 ccttttaacg aaaaatttcc tctttatggt tcggaagaag attttttccg aagggttaaa
     2461 ccgaagacaa tgattgtccc ggaaagcttt gtctttcatt ataaacacgt cagcgtggaa
     2521 agcgactatt ttcctgatca gaagttccgc agaaaaacga agagccttta gatcaagagg
     2581 ctggttatgg tgatctgaaa aaacgtcaca ctctgatctc ttatcagggc actccaaaag
     2641 agcgtatccc agtgccgtgc cgtccgtggc gttttgcgtc aggggagagc aggacgggat
     2701 gatccggaca cgttttttgg cattttagtg tttttatgga tgaacagttt gggtgaaaac
     2761 cgcccgaagc gctggccgga tattgtggct ttaagagaac caggtcagtt aaattaacgt
     2821 cgttttggtt aaaaaacaca ttatctttgt ttgtgggcct gtttgccttt tttccccgcg
     2881 ggacgttgac cgataattct tactgatttt attaagtaaa attgatatat cataaaaaac
     2941 tacttacatg aacctttggc ttatttattg cttttatcag tgataccgct tcatcagttt
     3001 taagattaaa taaagtcgat tgaacaggta ctgcatgcta aaagttttcc gtaaagaagg
     3061 gcaaaaaggt ttcacgttaa tcgaattgat gattgttatc gcaattatcg gtattttggc
     3121 ggccattgcc attcctcagt ttatcgccta cagaaaaaga agttatgtcg ctacactcaa
     3181 cagtgatgtt aagaatgctt atactttatc tctggcctat cttacagaaa atccggaggg
     3241 gacgttgacg gttgccgctt tggagaaagc cggctatgca tcttcagaag ggacaaatac
     3301 cgatgtgacc tggacagacg gttccaatta taccattgct gtaacggggc acgccaattg
     3361 gggcttggcg aaaaatagcg cggaaatggc tgttgtttcc ggtattgcaa cgcttgttcc
     3421 cgccacgcaa taaaaaattt gctgaaaggt tcacgattcc cccccccctg cggtttttaa
     3481 cctcgaaaaa cgcaacctca tcacatcatg cacaagccga tcttttttgt tgccgtgctt
     3541 ttgccggtgg ttttctatag catcctatag attcttttca taacgggttt taccggatga
     3601 tccggacagg cttttttggg agcggtgcct ttcagatagg cttctttgaa tttttgaggg
     3661 cacgacgttg tggcaaggta ccctgattgc ggatcaatga tggctgtttc tatgcctgcc
     3721 ggtggaacga tatttgccgg tgcggaccgg ccgtagaaag aacggatgaa acgtgaccag
     3781 atgtgcaacg ccccccgggc gccggtcagc ccggtatcgg ctcccgagtc gtatccgacc
     3841 catacggcgc agacgatatc cggtgtgtac cccacaaacc aggaatccct gttgccgttc
     3901 gttgttccgg ttttccccgc cgccggaaag taaatgccca atgcttttgc ttcggcagcc
     3961 gtgccgcgct ccagcacgcc ctgcatggcg taaccggcaa gatagatgat acggggatca
     4021 aacgcttttt cccgccgtac cgttttggaa atgatggtac ggccgttggc catttttacc
     4081 gagtaaagtg caaaaggatc aaagcggatg ccgcccgacg caatggtggt ataagcgtag
     4141 gcaagttcgc gcggcgtgac ttcaaagctt cccagtgcca tggaaggaac tgtgctcaat
     4201 ggacttgtga ttcccgcccg gcgcgctgct ttcagtactt ccctgaaacc gatttcgccg
     4261 gccagccgtg ttgtagccgt gttgacggaa tcttcgatca ttttacgaat ggtgatccgg
     4321 tcgtagtgct tgttttcgaa atttgtgggt ctccacattc cctcgggggt attgatggaa
     4381 agcggttttc cggaaacggt tgaagaaagc gtgatatcct ttccttgctc cagggaaagc
     4441 gaaagcgctg ccaggagaac aaagggtttg aaagcgctgc ccgattgacg gtttgcgtcg
     4501 accgcgcggt tgaattgcgt ttcccggtag cttcttccgc cgaccatggc gatcatttct
     4561 cctgttgcag gatcaacggc caccagtgcg gcctgaagtt tctctccggc agggaaagca
     4621 cgcctttcaa tttcccggat gccctcggcg agggcttcat cggcgatcgc ctgcacaaca
     4681 ggatccatgg tggtataata gcggtagccc cgatggtaaa gtttttcgct gcccagatcg
     4741 tcttttgtga tccgctgtat gaaatcgatg aaataggagg tttgtgcagg cgctttagag
     4801 agtttcacgc gcaccggcgc tttgcaggcc cgctgaaact cgccgtcgct gatcattttc
     4861 agatccttca ttctggacaa aacagcattg cgtctttctt ttgcggcctt gaaattcctg
     4921 tagcggttcg gggagtgcaa aataccggcc agcagggatg cctcttccag ggaaaggtgt
     4981 gccgcagatt tggaaaaata aaagccggct gcgcttcccg caccgtagat gctccgcgac
     5041 ccgtcctgtc cccagtatat cctgttcaga tacatttcca gaacctgttt ttttgtgtat
     5101 ttcagctcca gcacgaaagc gagttccgat tctcgcagct tgcgcgcaaa ggttttttcc
     5161 ggagaaagaa agaagtttct tgccagttgc tgtgttatgg tggaactgct ttgaggaaag
     5221 tttcgttttg cggggtcgga gaagaaggct ttgacgactg acggcagatc gatacccatg
     5281 tgtgaataaa aacgcgcgtc ttcggatgca ataacggcgt tttgcagaaa aggggaaatg
     5341 gcggaaagac cgactgcagg ccgggagggc atcttctcat cgatgatccg gacgatttcc
     5401 tccggctcca gttgaatgga cgtcagtgtt ttaccggtgg acgatataat ggcatcaacc
     5461 cgtccgtcac gcaacacgat ttcgaccgga ccgttatctt ccgaaggatt tctgtccgga
     5521 ctgtttttca gaaagatccg ccatcgcgat tgttcctcgg agaaggtccc ggctgcgtaa
     5581 ggagggccgg ctactttttt ataggaaagc ctgcgcagcc gatcgggtaa gcgcatgttt
     5641 tcgagatgca cgcccttttt gataaccgtg ctacgcccgt aaaaaatcga agggccttcc
     5701 gcggtggctt ccgacaggcc tctgccgaca tcgaaaaagg tatagccgat gcggaaaagc
     5761 aggatcaaag ccagcaatgg aagaatatat ttgcgcatca ctacgatttc tctcccgctc
     5821 ttataggcag atcagcgatg atctgttctg ccggaccgaa tttgtcatcg ccgtgaaata
     5881 aaaaaatttc ccggcatttc ttgcagatcg cggtcttttt ggcaagacaa ttttatggag
     5941 gtcattacag gatgacatca acctgatgat cgatgccgtc attggtcaac aagacggctt
     6001 tttcttcctg ccggataccg tcgagcaata ctgtcttttc tttttttctt tccggcgtca
     6061 ccgtaacaac gatccgatag aatgtctttt tgtatcggta gtgcatgctg aaaccgcccc
     6121 actcggcagg cagacagggc tcaatatata atgtgtcgtc ttcctttttg aagccgagca
     6181 gagattcggt gatcaatctg tacatccagc cggccgctcc ggtgtaccat gtccatccgc
     6241 cccgtccggt atgcggcgaa aacgcgtaaa catcagccgc gacaacgtag ggctccgcct
     6301 tgtagatggc tattgattcc ggagaatttg catggttgac gggattgatc atcgtcagaa
     6361 tttcccaggc acggttgttg tcgtgcaggg cggcaaacgc catggccgcc cagaccgccc
     6421 cgtgggtgta ctgtccgccg ttttcacgga caccgggaac gtatcccttg atataaccgg
     6481 gattcaaccc ggatttatcg aacggcggat gcagcagttg gacgatgccg atatcccggc
     6541 gaataagatg ctggcttaac gattccatgg ccagccgcga atgatggccg ttgccggctc
     6601 cggacaacac cgaccagctc tgggcaatgg aatcgatccg gcattcctcg cttctggcgg
     6661 aaccgagcat ggaaccgtta tcgaagaaag cgcgccggta ccagagtccg tcccagccgt
     6721 gctgttcgat attcaggcgc aattgcaggg cctctttcct gcagcatttt tcaaaagacg
     6781 tatccccgcg ccggctggcg attttcgcga attgtgtcag gatgtcatag aggaaaaatc
     6841 cgagccagac actctcgcct ttcccctgaa tgccgaccat gttcatcccg tcgttccagt
     6901 ctcccgatcc catcagcggc aatccgtgtt cgccgaaacg caatcctctc ttaatggccc
     6961 gcaggcagtg ttcgtagagg tttgcctttt ccggcgatcg aaccggcaaa tcataatagg
     7021 aatcttcttc ccggttgagc ggccggccgt tgataaacgg gatgatttca tccaggatgc
     7081 cctgatcacc cgtccgggaa atgtagcagc aggtggtcag aggaagccag agataatcgt
     7141 cggaacattg cgtgcgcacg ccgcggccca gggggggatg ccaccagtgc tggacgtcgc
     7201 cttcttcaaa ctggcgggaa gcggccagaa gcagttgctc ccgggcaaaa ttcggttccg
     7261 tgtaaatcag cgccattaca tcctgcaact gatcccggaa tccaaaggct ccgcccgact
     7321 gatagtagcc tgtgcgggcc cagagacggc aggacagggt ctgatagagc agccagccgt
     7381 tggtaagaac gttgacggac ggatcgggtg tttgaacctg gatagcatca agcttctctc
     7441 tccagtaatt ctgaaccgcc tgaagagcat cggcggccgt cgccggatcg ctgaaaaact
     7501 gtgccagttt gcgagcctca gtaacattgc gccccatgcc cagcgtaaaa acaacctctt
     7561 tttcttcccc ctcaagcatc tcaagaggaa cctgaatggc ggcgcaggga tccaggccgg
     7621 cgccgacttt tccggaaaga tactgacgtt tcatgacggc gggatcggcc agcgtcccgt
     7681 ttcgtcccag gaattccatg cggttgccgg taatggtccg tatggacgcg ctggagttga
     7741 aaaaacagat gcggttggga aattccatgt tgtaagaatt gcgtgcgaaa attgctccgc
     7801 tctgatgatc cgcttccgtg atgatatgca tggcattttt ctctctgagg cctcccagaa
     7861 cccattcgat gtaacctgtg atggaaatgc ggcgcgtccg ttccgttaaa ttcttgattt
     7921 ttaaaacgat gaattttacc gatgcatcca gggcggtgta gatccatacc tcagactgaa
     7981 tacatcgctc cgtgtgttcg aaaacactgt agccgaagcc gtgccgggtt gtgtaaggtt
     8041 ccagaccgcg atggggcagg ggcatcggtg accagaagtg gccgcgttca tcatcccgaa
     8101 tataaaaggc ttccccgctt ctgtcgctga ccgggtcgtt gtgccagggt gtaagccgga
     8161 attcgtgggc attgtccgcc caggtgtagg acatgccgct ttccgaaaca acggtgccga
     8221 aagtcgggtt cgccagaaca ttgatccatg gcgcgggggt aatctgggat tttgacgagg
     8281 tgatgacata ctccctgaag gacggatcaa aaccgcccag gccgttgaag aaaaccagat
     8341 cagagcgcgg gggcgccgct ttgggaaccg gcggttcccg atgcgtacga ttggggatca
     8401 tccggggcgc ggccgttttc aagccgccgc gctgtttcac ctggttggcc agtttgcccc
     8461 ggttgccgga aagaataaca tgagcgaccg tctgaatcag aatgcggtct tcttccggca
     8521 tctgatccgt ggaccggacg aaaattccgc cgggctgatc tttcagatcg gcgacaaagc
     8581 ccgacgatac aagacccatg atctgatcgt gaagcaactg acggtaaccg gcatgctcct
     8641 cattccagat tacgagatcg acggcaagtc ctttaagtct ccaataggcg tgagcctgaa
     8701 tgagttggcg caccagttcg atatgggact ggtcggaaac ccgcagcagg acgatcggca
     8761 gatcgccgga tatcgcgtaa ccccagaggc ccgactgccc ccggcgattt tccgagatca
     8821 cgccggcgcc ggcacgcagg gaatcgttgg gaaaaataag cgaactggcg atatgggaat
     8881 acaactgggc atcggcttct gttgcgttga tctgtcggag aagcacttgg ctttgcgtcc
     8941 aggccatttc aaaaacacga tcggcaatgc ggcgatcctg atatttgtcc acgagcgtta
     9001 tggcttcgtc ccgggtttgg ccgatgccga aaaccatgtc caccacggcc gattgttccg
     9061 gcccgagggt tatgcgggat ctgatagcaa cgatcggatc aagcaccgag ccttgactgt
     9121 cggaaagagt tccgaaaaga tcgctggggg ggcccatggc ctgcggatcc gccagtgtat
     9181 tgccgcggcc gataaaacgg gaccggtccg tttcatagga gatttgttcg atgtcctgcc
     9241 cgtgcacggc catcgaatga agcatccagg gattgatttc ctgttccgaa cgcggccgtc
     9301 tggtgcacac aatcgcccgg cgctgctcga taatttcggt ctggacaaaa agattggaga
     9361 acgcgggatg cagcttgtcc gcagcggagt gtgtcatgac cacctccgca taactggtga
     9421 tatcaattgt tttttgcaat cgtgactggt tggtaatcgt gacccgccgg agttcgatgt
     9481 cgtcttccgg tgaaacgacg atttccgtgt gcacgtcaaa ttcataatcg cgacgccgga
     9541 attccacccg gccgtccgag aaaattgcct cataattgcg gggtttttta agcgtcggct
     9601 gataggtggt tgaccagatg tcgccggttt taacgtcgct aagatagcag aaaatgccgg
     9661 agctgtcgca ggtgacgtct tcgcggaaac gggtaacatc aagatttttc caccggctgt
     9721 aacctccgcc ggcgttggtc agcatgatgt gatagcggcc gttggaaagc agttgtattt
     9781 cgggaaccgt cgtgtccggc gtgttgaaaa cgcgtacagg gttttcggag atttccggtt
     9841 ctttctgtgc gctgatgagt tcggaaggcg atgcataaaa cgcaacggct ttggggacca
     9901 tctcctgaag gagcaataga gcggactgaa atagcggttc ggcttcgaat cgtttttgca
     9961 tcggccggtc cagcagcaag taagtcaggg ccagcagggt catgccctga tgatgagcca
    10021 tataggatcg aatgacagca ttggattggc cccgtgtcag acgcgacggc gtaaaatcga
    10081 tggcttcata aaatccgaat ttgccctgaa attccctgga ggcaagtttc tccaggttga
    10141 gacaggcttt ctccggttcc accatcaacg ccagagcgga agcgtaaggt gccacaacca
    10201 aatcttctgc cagcccgcgt ttcagaccga ggccgggaac gccgaaagcg cgatactggt
    10261 aattcaaatg actgtcaaaa gcatagtatc ccgattccga tattccccag ggcacggagc
    10321 gttttttgcc gtactcgata tgcgcggcga cagccgcttt gtaggtctga tcgagcagcg
    10381 atccttcgta ggtcggcatg atcaggaggg gcatcagata ctcgaacatt gagccgctcc
    10441 atgaaacgag cacaggtttt ttcccgggag ccatggtcaa gagcctgccc agggcaaacc
    10501 agctttcctg cggcagtttc ccctgcgcga tgcccacaaa attactgaat ctggcttccg
    10561 ccgccagcaa atcgtagaag cttgtatcgc ggcgggaatt cgttacgtta taaccgatag
    10621 agaggagatt gcaggattta tcaaaaagga agtcatattc gatatcggtg aattccgtgc
    10681 attgccgcgc cagcaagtcg atgttttgca tcaaattgac ggcgcggttc ccggattcgg
    10741 caatcagatt tttcaggtcg ctgattcgcg agaggtcaat ggcggtcgat tcgaatcttt
    10801 tcattgcctc tgttttcgat tcaacgtgtt cataaaggct tgttaattct ctcagcgtca
    10861 tgatgtcatc gagttcgggc acttcccgga tgagatccga aaactgcgga tcaaacagcc
    10921 atggtgtgag aaagagcatt tcgtcaagag ccgctgttgc ctgctgaagg aaggccttta
    10981 accagtggcc ggcggcgttt gaagcatcgc tttccggacg catgcatgat gccgtttctt
    11041 cagccagcga tattattttc tccaggacga ttttcatttc cgtcaggctg gccggcggcg
    11101 aagccgtggc gtcatccagg gtcttgctga tttgggaaag atttgcggaa actgattttc
    11161 ccgtagagtc attcaggatt cccagcgtgt ccttgaggcc gtcaagtatt tgcgggtcga
    11221 tgattttttt atcgggcaag gaagacagtc ccgattttaa aataaaaagg tagccgacga
    11281 gattgccgct gtcgaccgat gagacgtaca ggggcaacag cggatccagg gactgcgtgt
    11341 cataccagtt gtaaaaatga cctttatatc gttccaggga ggtcatggtt cgcagggtgt
    11401 tggatgatct ctcaataaat tttaaaacgg tgatgtaacc ggaatcatat gcggtaaggt
    11461 tcgccagaag cgacaggccg atgttggtcg gcgatgtgcg gtgagcgacg acttccacgg
    11521 ggtgttcctg ataattgtcc gggggaagcc agttgtcttc gggaccggcg aattcttcaa
    11581 aaaacgccca tgtttttctg gatgcttttc gtagaaaatt aatttgggct gaagaaagtc
    11641 tggcttcccg gagtcgcgac ggctggctga accaccaggc gacggccggg gaaagcagcc
    11701 agagaataag acaaaacgcc gcggacggta cgccggtggg aactgctgtc aataatgcag
    11761 cgaatactgc ggcggcaaga gacggtgcaa accacatggt cctggtataa ccggcaagat
    11821 cgtcgcgttg attgttcgtg ttttccgacg gcgtccattc cagaagccgt ttgtgcgaaa
    11881 tcagcattct ccagagcgtg cgcagcaagg catccgtgtt gaaataggct tcgtagggca
    11941 ggcaaataaa tgaaaacaaa acctgtaaaa aattattgcc ggttttctgg aagactgagg
    12001 acaggtgatg cgtcagaatc acattctgct gtttttgaaa aacgctcatc aacgacatga
    12061 caagggccgg aaacacaatg atactcaaca cggccagtgt ccacaagccg ggcgaggaag
    12121 acagcagcca ccccagaatc agcatgagcg taagtgatgc cggaacaagg cttctgcgca
    12181 aattatcaaa gattttccac tgggaaagca cggaaagagg attgccgagc ctttccccat
    12241 cagcggaagg aaccctggac atgatccagc gggcgatctg ccagtctccg cgaatccagc
    12301 ggtgccttct gctgacatcg gctttgtatg ctgacggata gtcttcgtag agctgaacat
    12361 cgctgatgag gcccgatcgc gcatagcagc cttcgatcag atcatgactg agaatataat
    12421 tttcgggaaa gcgcccgttc agggatgctt cgaaagcatc gatatcgtaa attcccttgc
    12481 caataaacga accttcgccg aaaacatcct gatacacatc ggaaaccgcc ctggtgtagg
    12541 gatcgattcc ggaactgctt ccgtgcatga tgctgtaaag cgaccggctg atatcgggaa
    12601 gcgaaacaga gaccctcggt tgcaggatgc cgtatccctg gacaacgcgt cttttctgtt
    12661 cgtcatattg cgcccgattg agcgggtgag ccatggttcc cacaaactgc catgccgcat
    12721 cgcgcggcag tttggtgtct gtatcgagag tgatgacgta tttgacattt cttaattcct
    12781 gtgtgtcgcc gacaatcagt gagaacaacc ctccttcggg gccgtgcgtg ataccgcgca
    12841 gaaaggcgtt cagttcagcc agtttccccc ttttccgctc ataacccatc cagatatttt
    12901 ctttcgggtt ccagcggcgt gaacggtgaa acaggaaaaa aatatctccc cggtcatcag
    12961 cgtatttttc gttgaggctc tcaatccctt tctgaacgag gcgcagaagc tggccgtcat
    13021 cggacatttt ttcggttttc gcgtcgcaaa aatcggtcag aagggcaaag cgcagatttt
    13081 cgtcgcggtt cgccagaaaa cgaacttcca gcgattcaat cagtttgccg acgttcttat
    13141 cattcagcag cattgtcgga atggccacca gggtgcggta ttcgggagga atgccgcggg
    13201 aaaagtccat tctcggcaat ggagcgggtg ttatcagaag cgtcgacacc caattgatca
    13261 gcgctatgct taaatggctt gtactcagaa gggccacaag ccagataaca gccagcagcc
    13321 acccggtcag cccgtccgca taagccattg ctaacaaaag aaaagccgct gtaaacgtca
    13381 gcaggaagat ggaaccgaca taaatcaaca gcggaaatcg ccggctgaaa cgggcaacgc
    13441 tctcgaagag ggagaaacgc atcttggtca gacgctcgag ttccggcaat ccgttgtcga
    13501 ttaaatagaa accgacatgt gccgtacggt cctggccgcc cttctggttt gatgtcttac
    13561 gggcgagcaa tatcgccata cgcgccactt cggtttctga tagactgctt ttttttgcga
    13621 tgttttctat gacatgacgg taccggtcgc gggtggcaaa atccattttg acataaatat
    13681 ccgcgggatc ctcgcgcagg acctgatcca cgaaactcat ggtttccacg aattcccgcc
    13741 agttcatcgt tcccagaaac cgcagactga cgatactgtt gctcatggaa acctggtcag
    13801 ccgcctgctg ctgactttcc atctggacga aatttttaat ggtctgaccg tattcggaaa
    13861 gctgctgctc tatccaggtc agcggcaatg ccagagccgg gccctgtccc tgcagctgac
    13921 gggccatttc cgcaacaaag gggccggcaa ttggcggttc cgaacggacc atatcggcga
    13981 tttccagaaa aagatttttt ggatccttgt gaacggtggc gatcattttt ctggcccagt
    14041 aatcggccag gtcccggttg gttctgtctt tggagatacg tgacgcgata cgccggatgt
    14101 tttcgatcag cgccaggcgc aacataatag ggattgccca taattcaccg attttcagta
    14161 cggtgacgga ttgataggaa gaaacaaaca ggctcagggt tttcgaatcc acccgaccat
    14221 cgccgtggga tatcatttcc agcgctatgt catagacgcg gggaagattg gctgacggcc
    14281 cgttgagcag atggggcagc tctctgctgt agactttcgg gaggtgtcgt cttgccgttc
    14341 ggatctgctc ttcaatcaga taaaagttat ccagaaacca ttcttccgcc ggtgttacgc
    14401 ggcggttctc cagcaccgct ttggtcaatt tttcccgggc ctggaaaagc accttttcat
    14461 tttccgttaa tcgttcaagc aactggtttg aggtgagacc ttcgttcagt ttatgagcac
    14521 cggcaatggt tttgccgtgt tgtcgcatct gcttggcgct gaataattcc gaacgcagga
    14581 gttcgtcttc cgtcagtttt tcattctgat gtttgcccgc cagtctgctt tttaaacgat
    14641 gaaagtattt aaaaaaaatt attttttcca ataatgtgtg ccccctgtta tggcgcagga
    14701 tgatttggat tccatctctt atctaaagcg tttttataaa aatattgaaa aatattttcg
    14761 taaaaacctg cctgttgtaa cgattgtgtt atttgttagg tttaaagtat ggcaatgcaa
    14821 gttgcatttt tttcagggcc tgtttttcta tttgacgggc ccgttcccgc gaaatattgt
    14881 atttttcccc gatctcctga agcgttttcg gattgtctga catgacgcgg ttgagaataa
    14941 tgtaactttc tctctcattg agattggcca gcgctccggc aatgttccgt tgcaccaggc
    15001 ttttctcctc tttttttatc agggagatct cctgatcgtc tccttcgtac gccagaaaat
    15061 cgatgtgcga gttgctgctt tcgtcgttga tgatctcatg cagggagaca tctcgcgctc
    15121 ccatgcgcag gtccatttct tccacttcgg aatccttaac tcccagagat gtggcaattt
    15181 cactgaactc cggatttttc cgggaaagat tttccagttc ttttttcgtt tgattcagtt
    15241 tgaaaaaaag cttccgctga gcctgagtgg ttcctatttt gacgatgctc catgagcgga
    15301 taagataatt ctggatatag gcgcgaatcc accagaccgc gtaagaaatc agtctgtatc
    15361 ctttatacgg gtcgaatttc tttacggcat gaataaggcc gatattgcct tcctgaatta
    15421 aatcagacag tttgaaaccg tagtttcggt attcatgagc aattttgacg acaaaacgca
    15481 aattggaaac gatcagcttt tcggcagcgg agacatcgtt gtactttttc aggcgcacag
    15541 ccagtttaaa ttcctcctca gccgtcagca gaggaaaacg attgatttcc gctatatata
    15601 aattaagtgt gccggttact acgggaaatt ccaatgctgt cacgtctcct ttgccggtaa
    15661 ttcataaata ctagccaact ataatttcac ggtcaagttt tcttttttga aaaaagccca
    15721 gaatggatca aggaacaaaa ggattggact ccggcgaatc aatgcatcta aatcaatgat
    15781 tttttgaatg atccaacctt gacagaggcg tattttatgc ttattctaaa aacaatgaaa
    15841 accaaggtga atatcaatga aaacaataaa taaaacaatg attgatcatg ccgtcaaaat
    15901 tgcacgtgaa atcaaagcca acgcgctgct tgtctgtgct gatgtggtca gtgatgtcag
    15961 tctgattatg gatgaaatag aaaaagatat cgctttcatt atggtttccc gcgaggacga
    16021 aagcctatcg gaagaaatga aaaaagacgc gaagttgctt tatatcccga atgtgaattt
    16081 gacccgcgtg ggaataataa aaattgccat tgccaaggga atcattctgg gtgctctcaa
    16141 aagaggcgat aaggtggttt gtttaagcgg ggtgccgaaa ttcggttacg ccgacagcat
    16201 ttttgtgatc gatgtaggga aggaatttga gattctaacg tcggatttca tcaatgatgt
    16261 tctggaaaat gtgcgcccgg aagtatttaa tgctgtgctg aatatagcgc tggagctggc
    16321 cgctcaaggc agagaaagca gaaaggtggg aaccatcttt gttctgggtg atgacgagaa
    16381 ggttatgcag ctttcccgac agatgatcat caacccgttt ttggggtatc ccgaagatca
    16441 gctcaatatt ctgaatccgg atttggaaga gacgatcaag gagctctcgg ccattgacgg
    16501 agcgtttatc atcaaggaaa acggcgtgct ggtcacagcc ggaaggcatc tcagcgccgc
    16561 tctggatagc aaggatttcc cgcccggtct gggcagccgt catattgccg cggccggtat
    16621 caccagtttg accagagctg tagccatcgt catttccgaa tcttcgggaa acgtgacgat
    16681 attcaaaaac ggcaagctct ttgtgaccat tgaaaaaccc gttgagtaac aaggctcgct
    16741 gactatagca agcgtaagtg gcagcgaggc aatctgatca tgagacccaa gcttcagaaa
    16801 cgaagtgtgc tgaagcttgc aggacgaatt ataccaggcg agtggtgccg tggtaagccg
    16861 ggtgaaacga agtcagaagc gtaagccgaa ctatcctagg agcgaagcga tgtagggtaa
    16921 ggatactcgc tgagagcgag ctttgtgatg ctcgtccctg tcacctgaag gtggcgggat
    16981 aattcgctct aagattttcc gattactacg ttttggaaaa tcaaggctca ttaaagcgaa
    17041 tggagatttg aaaattagat tttgataaaa ggtaaacgat atgcgttttg ataaatttac
    17101 attgaaagtt caggaagcgc ttcaggaagc gcaaacatta gccggttcca tggggcatca
    17161 gtcaatcgaa ccggagcatc tgctggtttg tttctttcga caggaggatg gcattgccgg
    17221 ggcgatcatc aacaagctca atgcctcgtc acaaaaaatc gagaaggaac tcgacggtta
    17281 tctgaaaaaa caaccgagtg tcggcggtgc cggcgcggga caagtttacc tggggggcag
    17341 gctcaataaa atattcgaca aggcgatgac cgaagcggcg cagctcaagg atgaatatgt
    17401 aagcgccgaa catgtgctgg tttgtatagc cgaggaaaag gaaggccagg ccggaaaaat
    17461 tctgcgtcag gccggtgtga ccaaagacaa tattttcaag gtgctgaaag atattcgcgg
    17521 caatcagcgc gtgaccgatc cgaatccgga aggaaaatat caggcactcg agcgctatgc
    17581 ccgcgatttc aacgcgctgg ccagaaaagg ttcttttgat ccggtgatcg gacgcgacga
    17641 cgaaatccgc cggatcatgc aggttctctc acggcgcacc aaaaataatc cggtgctgat
    17701 cggcgaaccg ggtgtcggca agacggcgat tgtggaaggt ctggcgcagc gtatcgttaa
    17761 cggtgatgtg ccggagaatc tgaaaaacaa acgcgttatc ggtttggaca tcggcgctct
    17821 ggtggccggc gcgaaatatc gcggcgagtt cgaagaaaga ctcaaggctg tgttgaagga
    17881 agtcatcagc gccgagggcg agattattct ctttatcgat gaaattcata cgatggtcgg
    17941 cgcgggcgct tcggaagggt cgatggacgc ttccaatatg ctcaaaccgg ctctggcgcg
    18001 cggcgaactg cgctgtgtcg gcgcgacaac gttgaatgaa tatcgcaagt acatcgaaaa
    18061 agacgcggcg ctggagcgcc gtttccagcc ggttctggtc aaggagccga ccgtcgagga
    18121 tactattgcc atcctgcgcg gattgaagga acgctacgag gttcatcacg gcgtgcggat
    18181 caaagattcg gcgattgtgg cggcagcgac gctgtctcat cgctacatca ccgaccgttt
    18241 tctgccggat aaggctgtcg atctgattga tgagtcggcg tcgcgtttga gaatagaact
    18301 ggacagcatg ccttcagaga ttgacgccat cgaccgcaga atcattcaac tgaagataga
    18361 gcttcagtcg ttgaagaaag agtcggataa gaccgctaaa gagaggcgtg acaagattga
    18421 aaaggaaatt gccgaccttc aggaaaaaat ggaccagatg aaagtacact ggtctggtga
    18481 aaaagatatc atcaaaaaga ttcaaaatat taaaagccgg atagaatccc tgaagacgga
    18541 ggaggccaac gcccagcggg atggcaatct cgaaaaggct gcggaaatcc gttacggtaa
    18601 attggtggcg ttggataagg aacttcagaa cgagcagaaa aaactcgaag atattcagaa
    18661 aaacaatcag atgctcaagg aggaagtaga tgcggaagac gtggctgaag tcgtttccaa
    18721 ctggacgggg ataccgcttt cccggatgat ggaaagtgat gttcagaaac tgcttcatat
    18781 ggaagagcgt ctcaaattgc gcgtcatcgg tcaggaagag ggtatcaacg ccgtatcggc
    18841 cgctcttcgc cgggcgcgtt ccggtctgca ggatccgaat cgtcccatcg gttctttcat
    18901 ttttctcggg ccgacaggcg tgggcaagac cgaactggcg cgcgcgctgg cggaattcat
    18961 gttcgataat gaacaggcca tgattcgtat tgacatgtcg gaatatatgg aaaagcacgc
    19021 ggtggcccgt ctgatcggcg ctccgcccgg ttatgtcgga tacgatgaag gcggttatct
    19081 gacggaggcc gtgcgacgca aaccctacag cgttttgctt tttgatgaaa ttgaaaaagc
    19141 gcatcccgat gtcttcaata ttttgcttca gattctggat gacggacgtc tgaccgatgg
    19201 acacggacgg acggtggatt tcaagaatac cgtggtcatt atgacctcca atgtgggtag
    19261 tcagtggatt caggatccgt cgctggatga tgaagaaaag agaaaccggt cgatggatgc
    19321 gctgcgcgcc acgttcaaac cggaatttct gaatcgtatc gacgatatta tcatgttttc
    19381 ggcgttgaag ctggaggata tttacaagat tgttgatatt cagatgggtc tcatcgataa
    19441 gcgtctcaag gaacgcaaga tgagcgtcga attgacagaa aaagcgaaaa actatatagc
    19501 cgggcagggg tatagtccgg tttacggtgc gcggccgctg aaacgctcgc tgcaaaagct
    19561 gattctggac ccgttggcca tggagattct ggcggggaaa tttgccgaag gcgataaaat
    19621 tttaatcgat caatccaaaa aaggcgaaat aacgttgacg aaaaagtctt aaatattttt
    19681 cgcagaaaag gggaggaagg aattcatgga agacaagaag caaggctccg gatttgtgat
    19741 taaagacaaa cgtcatttcg gagaatcagc cgaagagcaa aaacaggaag aaaccaaaac
    19801 ggcgggagct gaagaaaaac aaaaaggccc ggagccgact aaacagggtg aatctgatta
    19861 tccgccggtg aactttacca atttcattct ttcattaagt acgtccgcgt tttttcattt
    19921 tggcgatctg gcggaatcgg aagacgccgc gccgcaaaag aatttgccgg cggccaaaca
    19981 gacgattgat attctcgata tgctcaatga aaagacaaag ggaaatctcg acaaaaatga
    20041 aagcggcctg cttcagggga tcatctatga actgaaaatg cgttacgtca aagaaaaggg
    20101 ttgatatgca ctggattgcg ccggcgaaga taaatctttt tctgaagatt ctgcgacgac
    20161 gcgctgacgg ataccatgac attttttcat tgatgcagaa aatcagtatt tatgatgaac
    20221 tgattttcgc tcctcttcat cggggcattg ttctgcattg tccggggatg aatctgcccg
    20281 aaaacgacgg gaatctcatt gtgcgcgctg caaaggctat ttttgcctat gccaattatt
    20341 ccggcggaat cgaaattact ctcatcaaga aaatacccat gaccgccggt ctgggcggag
    20401 gaagctccga tgccgcaaca acactgctgg ctttaaacaa aatatgtgca ttaaaactaa
    20461 aaaaaaatga gctgatggca atcggggcca aaataggagc cgatgtccct ttttttgttt
    20521 ttggaaatac cgctctggct accggaatcg gcgataaact caagcactta cgcaatctgc
    20581 ctcctctgaa tcttgttttg attaagcctc ctttcgatat ttccacaaaa atggtttacg
    20641 aaagcctcaa tttaagattg acaatgggaa aaaataatta tagcattcca cgatttttga
    20701 aactgggcga catagttcag gtattacaaa atgacctgga gtcagtttcg ttgaaaattt
    20761 atccggaact ggatgaattt aaaaaaatgt tgctcggaca tggtgcgctt ggcgcattga
    20821 tgtcgggtag cggccccaca atctttggga tatttaggaa cggaaaagag gcaaaaaaag
    20881 ccctggaatg cataaataag gaaattcccg gtaattatac ggtatttttt gccaagtctt
    20941 tgtaatcatt ccatattcct aatcagattg tgatcgatac ccctcattga gaaatatgtt
    21001 tattggggcg tcgtcaagcg gtaagacaca ggattttggt tcctgcattc ggaggttcga
    21061 atcctcccgc cccagccaga aaaaaatgcg gagtattttt taaatgcttg aaagaattag
    21121 aattttttca ggaaatgcca acaaagaact tgctgagaaa atttgcaata cattaggcgt
    21181 gtctctggga aaggccaatg tcaccacctt cagcgacggg gaaacccgcg tagaaatcga
    21241 tgaaaatgtc agaggaatgg atgtctttat cattcagtct acgtgcccgc cggtcaatgt
    21301 aacattgatg gaattgctga tcatgatcga tgcaatgaaa agggcgtctg ttgatagaat
    21361 cactgcagtc gtcccgtatt atggttacgc caggcaggat cgaaaagtag cctcccgcgc
    21421 tcccattacg gcaaagttgg tggcggattt gattacggcc gccggggcaa accgcgtcct
    21481 gtcgatggat ttgcatgcgg gccagattca aggatttttc aatattccgg tggataatct
    21541 ttttgccaaa ccggttctgc tggactatat gaaaaaaacc tacatggata atacggtgat
    21601 cgtttcgccg gatgccggag gtgtggaaag ggccagatct tttggaaaac gactgggggc
    21661 atctctggcg attatcgata aacgaagaga aggccccaat gaggcggaag ccatgaatat
    21721 tatcggtgat gtcaagggaa aaagggtgat cattctggat gacatgattg atacagcggg
    21781 aacggttgtg caggctgcaa gcgccatgaa gaaagcaggc gcgctggaaa tttcggtgtg
    21841 ttgtacgcac cctgtgttgt cgggaccggc tctggatcgc attgaaaatt ccgatattaa
    21901 agaagcgatt gtcaccgata ccataccgct gagcgaccgg gcgaaaaatt gcgggaaaat
    21961 aaaagtgtta tcggtatccg gaattttagg cgaagccgtg cgacgtattt attacaatga
    22021 ttccgtaagc tcactattta tataggaatt tatatgagga ggaacaaatg gaggtaacag
    22081 atttagcggc tcaagtccgt aaagaacaaa agaaaggacc atcccgccgt ttacgggata
    22141 agggatttgt cccggcggtt ttttacggcc gctctgctga aaatattctg ttggctgtaa
    22201 aaagcgatga actgattaaa ctgcacaaag acaaaaaaga tcacgcattc attaaactga
    22261 tcattgacga tgaaggaagc aaaaaaattg aaaaactgtc tctgattaaa gaactgcagc
    22321 ttcagccgct gaccggaaaa ctctaccatg tcgatttcta tgaagtggat gaaaaacgca
    22381 agatcaccat ggatgtggcg ttgaatttca tcggcaaagc catcggcgtg gaaaacggcg
    22441 gtgaattaca gcacttgaaa agagaggtca aagtttcctg cctcccgctg gatcttcccg
    22501 atcatatcga tgttgatgtg accgatctgg aaatcggcga ttccgttaag atccgggatc
    22561 tgaaagtggc tgaaggggta acgcttctgg acagacccga tacgtcggtt gcctctgttg
    22621 ccatgatcaa ggttgccaag gtagaagagg ctgccaaaga ggaaggcgct actgaaggcg
    22681 ctccggcgga aggtgaagca gcggcaacag ctggcgctgc ggctgctccg gcagaaaaag
    22741 aaaaggggaa gtaactttat agaaaagtgc aataattggg tgatagcggg tgcttctctc
    22801 ggttttaaag aaaatatttt tccggcaagt tgacgagagg gagcaaatca tgtatctgat
    22861 gatcggactg ggcaatcccg gaagccgcta tgcacggaca agacacaaca tcggtttcat
    22921 ggtgttggaa aaaattgccg cccaatggaa aattagttta acgcaaaaaa gttttgatgc
    22981 tctttggaat cgaggtaaga tcaacaacgt tagcgtactg ctggcgatgc ctcagaccta
    23041 catgaatctt agcgggaaat cggtcggtcg attaatggcc tattacaagg tggacattga
    23101 ccatgtcatc gtcattcatg acgatcttga tctgccgttc ggaacgattc gtctcaaaaa
    23161 aggcggagga gatgccggac ataaagggct gaaatcggtt atcaccgctc tgggatccgc
    23221 ggatttcctg cgaatcagaa tgggtatcgg caaaccggcg gacaaagccg gcgttgaaaa
    23281 ttacgttttg cagaagttta atcaggaaga acagggtgtg cttcctgaaa gcattcaact
    23341 ggcagcggaa gctgctgcgg acattgtcat atcagggatg caacaagcta tggctaaata
    23401 ccatacaaaa aatattaagt aattttaaag gaggaatgga atgtttaaaa acaaaaaaag
    23461 tatctggacg tttttactga gcacattgat gtttttgctc atggcgggcg cagcgtttgc
    23521 cggtgaagca gacatccaat tgccggatct gacccaagtt gcatttatgg gcgggtctct
    23581 tcagggattg acaatcctca atgtaggatt gatcatctgt ttaataggaa tggctttcgg
    23641 catcatgcag tatgtgcaaa ccaaaaacct gcccgcgcac aaggcgatgc tggatgtttc
    23701 ccagaccatt tgggagacct gcaaaaccta tttgttccag cagggcaaat ttctcatcgc
    23761 gttgtggatt ctgattgcga tctgtatcgt ttactatttt ggcgctctgc agggaaaggg
    23821 agtttcggac atcatcatta ttctgatatg ctcgatcttg ggtattctgg ggtcctacac
    23881 ggtggcctgg tttggtatcc gtatcaatac cgtcgccaac tcccgcgccg ctttttcttc
    23941 attgagcggc aaccctctca acatcgttaa catttgtctg cgttccggta tgagcgttgg
    24001 ccttcttctg gtcagcattg agctcttctt tatgattatc attctggcct acattcccaa
    24061 agaactggcc ggccccagct ttatcggttt tgccatcggc gaatcgctgg gcgcttccgc
    24121 tctgcgtatc tgcggcggta tcttcacgaa gattgcggac atcggttccg acctgatgaa
    24181 gatcgttttc catcttcccg aagacgatcc caaaaacccc ggtgttattg ccgactgtac
    24241 cggtgacaac gcgggcgaca gcgtcggccc cacggcagac ggttttgaaa cctacggtgt
    24301 gacaggtgtt gcgttgattg cttttctggc tctggctttg gccagcaatc cagaaatggg
    24361 cggtaaactg atcgtctgga tttttgccat gagaattctc atgatcttga cctctcttgc
    24421 ttcttatttt gtcaatgacg ttatcagcaa ggcggctttc ggtggcaaga aagaatttaa
    24481 ctttgaacat ccgctgacca acctggtctg gattacctct attgtttcca tagcggttac
    24541 gtttgtcgcc agttattacc ttctgggcga tctgactgaa ccttacacaa gtctctggtg
    24601 ggcgctggcc atcattatca gttgcggaac catcgccggc gcattgattc cggaattcac
    24661 caaagtattc accagtacgt cctcccgtca ttgcgaagaa accgttaatg cttccaagca
    24721 gggcggtcct tctttgaaca ttctgtcggg tctggttgcc ggtaacttct ctgctttctg
    24781 ggaaggcctg accattttga ttctaatgtt cattgcttac atggtatcca agaatccggc
    24841 cgttatggca ttgatgcctc cggaattcac ctttgctgct cccgtgtttg ctttcggtct
    24901 ggtggccttc ggtttcctgg gcatgggccc tgtgaccatt gctgtggaca gcttcggacc
    24961 ggtttcggac aacgctcagt ccatttacga actttcattg attgagtcac gtcccgacgc
    25021 aaaagacgtt gtgaagaagg aatacggcat tgcgcccgat tttgaactgg ccaagcactt
    25081 cctggaatct gctgacggcg ccggcaatac cttcaaagcc acagccaaac ccgtgttgat
    25141 cggaaccgcc gttgtcggcg caaccaccat ggtgttcggt attatcattc tgctcgaaca
    25201 tctcttcgga aatgttgttg ccaaattgtc tttggttcag ccggaaatta tcctcggtct
    25261 catcatgggt ggcgcggtta tttactggtt caccggcgcc tccattcagg cggttaccac
    25321 cggtgcctat caggccgttg tcttcattaa gaaaaacatc aatctcgata aagcggaagc
    25381 ttcgattgaa gacagtaaag aagttgtcaa gatctgcacc cagtatgctc agaagggtat
    25441 gatcaacatc ttcatcgtga tcttcttcat gtgtctggcg ctggccttct ttaaccccta
    25501 cttcttcatc ggttatctga tcggtatcgc tttcttcggt ctcttccagg cgatcttcat
    25561 ggccaatgcc ggcggttgct gggacaatgc caagaagatt gttgaagtcg acctcaagat
    25621 gaaaaacacc ccgctgcatg aagccagcgt tgtcggcgac accgttggcg atcccttcaa
    25681 agatacctct tcggtttctt tgaacccggt catcaagttc acgaccctgt tcggattgct
    25741 ggcaacggaa atcgcggtga ccatgaccaa cccgaatctg aagacgggtc tggctatttt
    25801 cttcttcctg atcgcgattg ttttcgtgta tcgttcattc tatagcatga gaattgccag
    25861 tgaaaaactt ggtgaataag aagtactcaa ctctttaaac caaaaggcgc cctttgaaaa
    25921 aggggcgcct tttttacatc gattttggct gcttcataat attattgact gttttttgaa
    25981 gtgcttttgc aatccgatca tatccatgtg ctttggtatc cggtctccat ccggccaggc
    26041 gttgattaaa gctgcataac caaaaggatt gcgatggcag cttttccctc ctgcgtatct
    26101 gattgttttt cagatatcct gatgaacgtg aaacagatcc ctcaacatca tttctgtccc
    26161 aaagtatgga ttgttcaggg tgttgagtcg tttgtcattg gagccgagca ttttgtttag
    26221 aaacgtcact tcccgttcgt ccagagcaat attttccttt ccggcaatat aataaagatc
    26281 gttaaagacg ccggatattt tcggaaaaac atccgggaat acagccttaa gagtattaaa
    26341 attaatgacc tgcgcttcgg gtatgggcct gtaggaccat tgcgtaaaga tcccgttctt
    26401 atggagcgct tttttgacca gtttgtaaaa ttccagcgtg tagagattgc ttgaataaat
    26461 aacggacgga tgctcgatat ccatgataat caggtcatat gttttgtcgg tgcccagaag
    26521 atacttgtaa ccgtcgtcaa aaatgatttt tactttgggg ttttccagaa catgatccgt
    26581 gaatgcgctg aagcattttg cggcttcggg cattaccggg ttaatttcta ccacatcaac
    26641 agctttcacg ttcggatgct tgacgatttc gtgaagcgta aaaccgcatc ccagaccgat
    26701 atttaaaacc cgaagatttt tgttgtccag ggcatcgatg gagacattgg caaaattaat
    26761 ttcgctcact tctttttgtt ttaatcccac cgtcgagcac tgaacgcgtg tttcgatgta
    26821 taaatatcta tgctgttccg tgtccgcaac ttcatcaaac acgcttagct caccgaacgg
    26881 cgaatgagaa aaaaaaattt tttctttgat ttttactttc tgaaaatagg gggtgtcttt
    26941 cccggtatgg aaaagaagat tctcaccggg gatgtatttt tctttgttgt cgggactgga
    27001 ataaatggcc gtgtcgcttt taaacggctg aataatgaaa tagaaaacaa gcaacatcaa
    27061 agcgaaaccg gcgagcataa tgaggaactt tttgtttttc tgaaagaaaa caagcagacc
    27121 gatgatcagg ttgaccgaga cggaaaagaa aatcagggct ttgatgccga agaaggggat
    27181 gaaaacaaaa ccgctgaaca atgctcccag taccgctcca aaaagatcga aactgtatat
    27241 cgtgcctatt ttctgcggca attgctctat cgcattgata gacatgacga taatggccgg
    27301 aaagaccatg cccatcagaa ccgtgggaaa aataagatag agaaaacttg tgaacagttt
    27361 gttgaacagc agcatgatga catcgtttcc gaacaacggg taaaggaaat tgaaaaccga
    27421 tggaaccaga tcataatccg tgaaaataaa aacggcgtaa agagcgatca gcagctgagc
    27481 cgaaagaatt aatttgcgtt gatcggagat ttttttctca acccggctga aaataaaact
    27541 tcccagcccg ataccgaaaa gaaagatgga gatcacagtg gaggtcgtaa tggtgctttc
    27601 gttgaaaaaa taatacagta cttttgttgc aactatttca taagccagac tgacaaaacc
    27661 ggccagaaac gcgataatga gcaggacgcg atacgtcggg taacggctgt tgtttttatt
    27721 tatcattgct tttttacgaa aactccgatc aataatctca gtatcttccg gaggccatgg
    27781 aatcggacag aaaaggatat cttaaattcc cgggaacgat aaaccagttt tatctgatac
    27841 gctattgaaa catccggatc aaggaaattc aattaatatt gtttgtcggt aaaatcgtaa
    27901 atatccggat gtgtgcaaac ctcatagtat ttttttgtta tctttccggc tgcatccaga
    27961 gcgctaggct gtaatttgtg aatgggcgct tcccggatgg caatccgctg ataattgccg
    28021 ttggtcaacg gtaccttccc cgcatacaat ttgatatttt catcggtttc ggaaggaaag
    28081 atataggttt gaaggttaat ttcccgggtg agtttatgga agccctcttc attcagttca
    28141 atcccgttga cgttgtagcg atacgtggcg tcttctcccg ccgcttcaat gatttcttcc
    28201 gcttcatttt caaacacaaa caaattgaaa atatcattct ggcttttaaa tatttttcgc
    28261 agtaatttgt cacagctgga caggcggtcc gcctgattga tggcctgtga gatcatgatt
    28321 ttggaatcgc catcaaacag gaacgtcggc ggatcctgag agtaacaaat gccgatgccc
    28381 agttcaagtt ccggaagatt gttctccttg tttttttcat tataactatg aacaatgcgc
    28441 aggattctga tggccagacc gcacgctctg gccacactgt aatttccttc cgatgcgtcc
    28501 tcattttcga aaagggacag gataatggcg tccccttcaa taaagacctt ggaggccccg
    28561 tattcggcaa gaacagccga tatgggatca aaaaaattca ggctgaaata ggaggccgga
    28621 ttcagcccca tttttctcat ggtatgattg atcgtaatag atccgcggat gtccgcttta
    28681 ataatgacat ggttggcgat gggtttttct tccgtaattt tttcgtcggg cagcagaaac
    28741 tcaaaaagcg agtggttttc ccgtgataac agaataattt tctcctccgt aacgaggtta
    28801 atggaattca tcgcatcctg gataagataa aaattataca ggtcgcggta gtatcgggaa
    28861 aattgtttaa gaaacagaat caaatacaat tttttttctt ttgtggagca gtctttgatg
    28921 tgtttgactg tttgtaggag agggccgaaa ttgaactgac taccgtaaag ggatttcaat
    28981 cgtttcaggt tctttctcat ggatgttctt ttccagaaat taaccagaaa ttctcttacc
    29041 tgccggggag acaacggagg acaatatttt tcgtagacgg atttcatctc gaaattggcg
    29101 acaatttgag gcagcaggcc ggcaactttg aattgatgat aaaaaagatt caacaggttt
    29161 ttctggctct cttttcgttc ttttaactta ttgagaatgc tttcctgttc tttgttgttt
    29221 tttgcctttt catattgttc ctctgtctca tagaagtcga gcatcttgcc gatattatcc
    29281 ggttccatga tcaacgcatc atataaacaa ttctccgcat cgaccaggcc gtgaaccgtc
    29341 tgaaattttc gctcattggc ttctgtcgca tttcggccgt cttcgtttgt taaatcgatt
    29401 tgtccgaaca ggtcgtacag gatcattttg atgctcctgt aattgtcagt atcttcggac
    29461 cgctgcccca tgagtacata ttcttcgatc agaaaaaaat catcgctcgg attattcgtg
    29521 tgcagcattg gatttgccag aaaaaagccg ggcaaaatgt cccgcggagg aaaatgcgtt
    29581 tcccgcatat ctctgagctt ccgcgtgttg acatcggcta agagctcaaa gagctctttt
    29641 cccgcgaggc gggtgatgtg atgatgatgc cgttttattt ccgacagctt atctttgatc
    29701 atgaacacat cattctggtc atgtttggtc gacaactggt acatcttgac cagaggttcg
    29761 atttgttcca ccagaatgtt gtactgtaac tgaatttctt ccagaaaaag ttttgccaga
    29821 gccgtctgcg ccagcagatc gatctgcact tccgacgctt ttttcgcttt attgattcca
    29881 tccagcaaaa catcgttgca gattcgtctc agggcgtctt tttccgattc acaaaactct
    29941 tttttatatt ctttgaaaaa gaattcagcc ccgctgtgtt ttaccatcaa gagaaaggct
    30001 gtctttttaa gggcgttatg cacctgggag ctgatatgga catccagatg aatgttgtcc
    30061 actccgggag aaagtctatt cagagacgca tagatctggt agtttttaac acccagcgtc
    30121 ttaatcgtcg tcggtttttt gaatgatttg aaaatattaa aacttatcat gacacccttt
    30181 atgcttattg gataatcgaa tcactattcg gtccgccaat aattcatgaa acggatacga
    30241 tgcatgaata attttcacga tcagatcaat gccggcctag agacagtatc cgcgactttt
    30301 aatcaaatcc aacgccttac aaaatccaag ttcacaggcc ttcttcacgt cgctgcatcc
    30361 ctgcgtgtta ttttccagca taaaataagc caggccgcga ttgttgtatg cattggcatt
    30421 acggggttcc agtttaatgg ccttgttgaa atcctcaatc gccattttgt agcttctgag
    30481 cttgttatga ttgatgcccc gtgtattgta tgcttctgca aaatccggtt tgagacggat
    30541 ggcttcgccg cagtcggcga ttgcttcttg atactggccc agattgtttc ggacggttgc
    30601 gcggttataa taagccagaa caaaatccgg ctgcaagcgg atggccgcat taaaatcttc
    30661 cagggcaagc tgattgttcc ccagattctt ataagcgact ccccgctggt tgtaggtatc
    30721 ggcgtcatca ggctgcagct taatggcttt gcttaggaat tcgactgcct tttgcggttc
    30781 cgtatattgt ttgccgtccc acaagaggtt tgcttcattc atcatatctt cagcattttc
    30841 agaactcttt gagcaggcca tcaggctcaa aagaagaagg ataaggaaaa ccgttttttt
    30901 cataatatct gcctccataa atagtgttta gtatgcaact tcactagcat aattatcgga
    30961 aaaatataat aaaaatattt ctgttacttt agacgcagaa aaaactccgc cggatgaatg
    31021 tgaaattttt ccggcgaaat tgttcggagg atactcaatc ggtctttaac ctccagagaa
    31081 ttttgatccg ccagataaat catctttccg ctttgccagg ttcctttccc gtcatcttca
    31141 agaagcataa taagcggaag actgcgttca gtttcttcag ggtaatcaat caccacgccg
    31201 aaggatccgt tgttgatctc tacaacggat cctatagggt atgttcccat catgtgaata
    31261 aagcgcttca acagaatcgt atcgaagctt gtccccgctt ctttccacat ctctttaagg
    31321 acttcatccg gcatgaaaga ttttggccgg taaaccctcg cactggtcat ggcttcataa
    31381 acatcggcga tatgaataat cttgcccata aggcttacat tattgataaa aagcgtttga
    31441 ggataaccgg tcatatccag attcaggtga tgctcgaaag gcccgagaat gatttttgat
    31501 cttaaggacg gattggcatt caacatgaga atttttttga cccccatgac agggtgtttc
    31561 ttgaccgtgt cccattcccc gttggtcaac tcaccttgct taaagagaat ttccttggga
    31621 acgtccactt tccccaaatc atgaaacaac ccgcaaatcg tcaaatgttc cagcacgata
    31681 tccgataatt ctatatgtct tcccagacag gcggccagaa gcgcgacgtt tacggaatga
    31741 atgtaggtat aatcatcata atccttgagc gttgtcaggc cgatcatcaa cgcggcatcc
    31801 tgctttgtca gatccactat gttttgtgcc aaacgcctga ttctgcggac tcccgccgtt
    31861 ccatgggata ctttattcgc cacttctttg accgcctcga tcgcctgaaa ataatttttt
    31921 cgcgcttttt caagtttctt ttcttccagg ttttcccctt cggcgatgat gtcttcatcc
    31981 tgaagttgct gaatctgcac ccatgaaaaa tcatttttcg tcaattgttc ttcaagccat
    32041 tccacatgac gatcgtgctt gacggcctca ttgagcagac gtgccagggt catgattttt
    32101 tcgggcggca catcgcgtga ggatgttaaa aaggtgaggc ttccgatgct ccgctttgag
    32161 aagaaatcca ccatattgta aacaacaacc gctgcatctc tgcgataggg aattttttct
    32221 ccgcccagat gaaaccggcc tcggttaaga cggaggctga tatctccgtc gtttgtcaat
    32281 tcatggagga tcttatgaaa agactcgacg cttgtacgga caatctgatt gttgtcctga
    32341 tacatgcgaa ctgtgctggt catatgatac agggttctca gaaaatcatg tccccattgt
    32401 tccgaaatcg atttttgttc cgacatgatt atgatccttt tttgtttttc tgaacaactt
    32461 ctttttctgc tttccgggcg attctgcgca ggctggggct gaaactgcgt cccgctgcct
    32521 caatgatccg actggcttcc ggcattttta gagccgccag cgcaaacgcc gccccctgac
    32581 ggcataccga tttctttgcg ccggcgatcc gctttcgttg taacaggata tctcgtaaaa
    32641 agggaatgca acgctccgat ccgcacctgc ccagagtaac caggcactcc gaaatatacc
    32701 aggcctcttc gttatcgaat tgatggttct gaagatattg cagtaagaga tcttcagccg
    32761 cctcgttcct tgttctgctc atttgcttca aaattgtccg gcggaccgat tcatccggat
    32821 cgttgaggaa ttcgaagatc ctggggatgt aatccccctc gatttgcccg atggttttga
    32881 cggccagccg ccgggcggac ggcgaaggat gtcgcgcaag tttcatcaga tacttcaggg
    32941 aaatattccc ctccactttc gacagcacgg aaacaagctt ttcggcgact ttttcatcgg
    33001 cctttccgat caactggttc agcggattgg tatcgcgatt gatgagggcg ataatagtat
    33061 cctgcagaag ttgctccagc tgtgacggct gccctaaaag cagaaattct gtcagggtat
    33121 ggattgcctg cggttgaaga tgctgaaaaa tgtttttaaa gatttctatc tgctgtgcat
    33181 tcaaggaact ccaggcatcc ttcaacggtt ccaggcagcg gtcagccgat gaaatatctc
    33241 cgtaaagttt ttcgattaag tcgtgcgcgt aaggggatct caaacggccg ctatccaaaa
    33301 ctctgcggag tccgtcaaga ataatcaggg ttgcttcaaa atcatgacgg cgaatcgaac
    33361 cgtcaaattc ttccgatatg acttcgagga tgatactgaa atctttctcc tccttatact
    33421 gcagaagcat gtccagcaac atattcaaat gttctgtcgc ggacaacatt tcttcgcggg
    33481 caatcatgtc ctgcagggca atttgctctc tggctgtcag cacaaaagaa tcgggatcca
    33541 gcccgatttg ctcggaagat tcgggctctt gtgcatcttc gtgtaaagcc gcggtttctg
    33601 atacgtcggc ggattcagaa ccgtctcctt gatcttccgg ggttgcctga aatttcgtca
    33661 tgctttccaa agaatccgtc gcttgttctg aaataaaatc gtcagcctga tacaggatat
    33721 gattgaaatg tgcttcccag aaagcggtga cgatatcccc ttccggttcc ttggtcagta
    33781 cggaatattg gtgaataatc gttaataatt cgcgggtttc ctccagcgtt atcccttcgg
    33841 cgaattccag ccatcgtatc ccgtcgcgga aaagcgtaaa aggcagggat ccttcttcag
    33901 gcggtcctgt ttgaacgttc atctcctggc aggtgacgcg ctccctttca aattcaaatc
    33961 ggatatcgcc gtgcttgcga atgtaggctt ccagcgtttc atgaaaattt ccgatagaat
    34021 tcagactgat actgtgacct gccggatata aagaaacgtt cttgctcgtc agcagaagct
    34081 gaacgaataa ttttttcatt tcttccgatt tttgaatgaa aagggctttt tcggattctt
    34141 tgctcatgtt atcttggaca ttcatttcgg taccgctggc gatgaatcat tttttattga
    34201 tgatgcttgt accatcctga taaagttttc aaaataacgg acaaaagcaa agccggtact
    34261 tgatctcggt cctgaagcgt tctttcaggc actttaatct gtggaatcac aaaaaaactg
    34321 cgatttttga gtgtaacggc aattgctttg tatgtaaaaa gtatagaaaa attagttttt
    34381 tctgtcaaga caatcttcat agcctaaagt cttatcttga atatctttaa tacggcttga
    34441 tgagcaagaa aaaagggtgg tgctttcgcg ccaccctttt ccttttgatt attggatact
    34501 gttaattctt atgggtcaaa agatagtcgc cgacgtctcc gtcaatccat ttccacaacg
    34561 ttccttcccc attcttgttg gcaactttga aatcaagttt ggcctgatga gcctcatcaa
    34621 taacgatggt gtttttcgac ggccgaagca caggaaacaa gtgggggaaa tcggaagcgg
    34681 aaatactcac gcggaggcgg tgccccttct tgaaaacatt ggttgtcggc cacaattcga
    34741 ccacatactg atagaccgtt tcttcagcaa tattgtttgg attcttttcc ggttgaaaat
    34801 agaaaggatc aaatgctctg tatgccggat ccacctgcgt aggtttagcc gcattataag
    34861 ggcggaacgc cgcgctgagc catccggatg taatattttt tgcccgacca ttggggaaca
    34921 catcattgac ctcgaccacc cactggacat cttttctatc ggcaatctgt aagatcatat
    34981 ccttgtcctc tccaatgttg aacgtggatt tgatctggtc aatgaagtta tcgagagccg
    35041 cctgtttcag cggctcggta aactttgttt ttgcccagaa ggtcagcttc aacggaccgg
    35101 aaatttccat atcactggcc agcggttcag tcgaaaaggt cagaacaccg gtttcatcac
    35161 tgcgctcatc ttcccagaat agtgtgctgt caagattgat atccagtgtg tacctggaca
    35221 actggctcac aatcgtcaga ggcgagaatc ccagccagcg ttgagccgag cgggagaacg
    35281 ccccgttgag cgctgtcggg ctatgatata gaacagggtt cactttcggc tttttaatcc
    35341 ccaaaatcac gttattataa tctgtggtct tcacgctggg gaccagctta taattgtcgt
    35401 cataattggc aactccaaac caatcgccga aaatcagaga ggatttagct ttggaaagat
    35461 aatacgtttg gttgctgagt ctggatgaag gcaaaggcca tgatttttca gcacgccatt
    35521 tgttttctcc cagtacgtag agcagcaccg ggaactcctg caggaacgga tcgtcttttc
    35581 ccttgagtct ccagtccatc catcggcgga ggacatccca cgtgaaaata ccttccttcc
    35641 cttcgatgcc catccccttg acgccgggaa acataaacgc tgcatcaata tgataccagg
    35701 gccccacaat gatggcctta ttgccgggag catgattttt caatccatat tcatagttca
    35761 tgaaagaacc tctggtgaag atatcgtacc agccggttgc ggttaggatg ggaagatttt
    35821 tcgggatgac gccgccgccc acggccgggg gcatatccgg aaaattccag ccgccggccg
    35881 gtttgtccgg ccagtaaatc atcggcgatt ttgtgtcata ccagctgttt ctcgttcctt
    35941 tggtcgaatc cataaaccat tccagcggca cggtcagttg tttgaaatgc tgtgcccaga
    36001 tgctcaatgc gttcatgaaa tctgttttgt tgaagttcgg ctccgccaca ccgccgacaa
    36061 tcaggtccgg cggaagaata ctgataaaat ccgtcataag gatccagatg gccatgaatt
    36121 ccagatcaaa attcccgccg tgccagacaa tgtctttcca gacgtcgtta taggccgaaa
    36181 tcggcgcaat agctttaaga tgcgttggag tgccgttgac ctgttcgaca agaccggcgg
    36241 caagatattg gatgatgccc atataggagc ctccgaccat gccgaccgta ccgtccgacc
    36301 acggctgcgc gggaatccac ttgtcaatca tataggccac atcatattgt tcaatagggc
    36361 tgagtggatc ccagacgcct tcaccggaac cggttccgcg catatccaac acaacgacat
    36421 tgtagtcatg gggcacaaag gcgatcacgg cgaacatcag accgatgagg tcccggtggt
    36481 aaccggttgc gagaaaaatc gtcggccgtt tgatgcttcc cgtgccattg taaggagacg
    36541 tatgataaaa agccgaggcg tagatattgc agttgtatcc gaccggattt tcagctttgg
    36601 tcatgggaat tgcgatgttg aaatcaacct tcacatccgc aacccccatt tttgccagcg
    36661 cttcctgtat ctgcgtttga gcagcagcat ccgaagtatt agcttgaaca tgagcggagg
    36721 ttataagaat ccccactgtc acagcgagga atatggacca gatcttgaaa cgatgccgcg
    36781 attggaacca tctgtttttc gtttctgcaa aaatcaaacc ctttaaaaaa caacctatta
    36841 caatggtacc acaacatttg gctagacgca gcaaatcaac ctgtttcatg atccctccct
    36901 ttcatggcaa tgttgtttgg cagtggattt tacaaactcc tccccccgtg ctttttttca
    36961 attggtaaga tattgatgag tgattggaac actaaacgat ttatcccgcg atgtcaatat
    37021 acttatggaa acgagatgtg atatttgggg actattcctg aaagttgtgt cggaagaaat
    37081 gagcgtgcaa aacgaaaaca ttgaaagaag gcgcacaaag aagatatttc atgggggaaa
    37141 tgtattattg cagaaggtag ctcaaagccc ctccaaaatg caaaaaaaga gctaactgca
    37201 tcagctaact ccttgtaatt acttggtagc ggggagagga tt
//
LOCUS       DAOSME010000021        36596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1530_length_36596_cov_24.828549, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000021 DAOSME010000000
VERSION     DAOSME010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 36596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36596
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_36596_cov_24.828549"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLG98_04790"
     CDS             <1..825
                     /locus_tag="PLG98_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOE32601.1"
                     /translation="RAAEDPLQEDLHMPVPKLVHRYPDRCLAIVTETCAVYCRHCNRK
                     RFWSRPASLNLTDRLQKITRYIAESPRIREVIISGGDPLTLDDRVLEKILASLKAIPH
                     VDVLRIGSRVPVVLPMRITRTLCRMLKRYRPLWFNTQFNHPGEITPEAARACNMLQEA
                     GIPVSNQSVLLKGVNDSPDIMGDLLYGLQKMSVRPYYLFHCDPAQGCLHFRTEVPAGL
                     DMMEKIWRHCSGLCVPQYVLDVPGKVGKIPLNILSETMKNDLKKHQHFFDKRQEID"
     gene            881..1444
                     /gene="efp"
                     /locus_tag="PLG98_04795"
     CDS             881..1444
                     /gene="efp"
                     /locus_tag="PLG98_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOE32602.1"
                     /translation="MYTASDLRKGLRLKIDGEPYVIVEFNFVKPGKGQALYRTKLKNM
                     INGNQFERTFRSVDTFEQADLREKTMQYLYKEGDKFCFMDNETFEQVYLTEEQVDDAV
                     NFMTDNIEVEILLFEDRPIGISLPNFVELVVTEAEPWAKGDTAAGSTKPVKVQTGYTI
                     QVPTFVTEGEKIRIDTRTGEYLTRVKG"
     gene            1575..2510
                     /gene="epmA"
                     /locus_tag="PLG98_04800"
     CDS             1575..2510
                     /gene="epmA"
                     /locus_tag="PLG98_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOE32603.1"
                     /translation="MNDFFQDNIFHPARKSQALEMRAKLIQGIRHFFINSSFLEVDTP
                     VRIPSPAPEEHIEALRSEDWFLQTSPELCMKRLLAAGYPRIFQICKCFRAGERGDLHL
                     PEFTMLEWYVAGFDYRQLMAQCRNLIVAVAQNQGLTEIKRDNRTIALTPPWESITVED
                     AFLRYAPLTLDEALGQGTFDEILVDCIEPRLGIGKPTFLYDYPASLAALAKLKDNHRT
                     VSERFELYIGGLEIANGFSELTDAAEQRKRFEEALKKRAEKKWSEYLMPEKFLQSLGH
                     LPPCAGIALGIDRLVMIITGANCIDDVVAFIPENL"
     gene            complement(2594..4099)
                     /locus_tag="PLG98_04805"
     CDS             complement(2594..4099)
                     /locus_tag="PLG98_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOE32604.1"
                     /translation="MKRKRILTQFLLYFLMLMFLIVAGCSSGGSDDDDDDQNQQQLFV
                     TVSGTVTDATGAGVQGVNISAMYSLTILNVKVWEGSISSDLFSYIITDANGNYTLRLP
                     AALVEQYNVILTPGKKGYTFTPARRTFDVGLTDITGVNFTAAEASVYGQADLAGTWRT
                     NMLRTGTENGWVRARLTIGATGVATCESYETSTSAGDTTCPADFDLTFTVNPDTGAII
                     QSGAYAAGDGGHMTMNSDMDFAAGTATAGTNYQIMIAQKEGTTNYSNANLASHNFVVH
                     GMVVGQTNEWWYGTGATDTALVTTMSEEVGPTGAPSATINGVTLAVAVDGVVTLVDAL
                     GARASDFEGFLSVDKKTMVGTYTDINGDYQLMIVQITDGQAVANLTGSSYNHLLGVGD
                     DVFWAHHDINVSRFETSFLPDILALTFNLDVMLSYNWNLSLAYTPIQKLGLVSVKKLT
                     IAPNGQAIVTNPETDAIVFHGQLAFDGTFMVGTETMELTEGTFYTLNVITH"
     gene            4271..4996
                     /gene="tolQ"
                     /locus_tag="PLG98_04810"
     CDS             4271..4996
                     /gene="tolQ"
                     /locus_tag="PLG98_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOE32605.1"
                     /translation="MKDFGALDLSGSVNGSLLSMILDAGLMVKFVLLVLFIFSVVSWA
                     IIFIKYRYYRKIQKENEAFNDDYLKSSKLSDVLPAAKKYSYSTTAEVFRVGYKELTQT
                     NRPAAGESAQGRDDISLSSLDNLERSMNKASATEMTKLESSLGFLATTGSASPFIGLF
                     GTVWGIMDTFKGIGARGSATLAVVAPGISEALIATAAGLAAAIPAVIFYNYFLNQSKN
                     MVQEMENFSAEFLNIVERYLVRK"
     gene            5014..5439
                     /gene="tolR"
                     /locus_tag="PLG98_04815"
     CDS             5014..5439
                     /gene="tolR"
                     /locus_tag="PLG98_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOE32606.1"
                     /translation="MVRGSRRKNNASKPLAEINVTPLVDVMLVLLIIFMVTAPMLSMG
                     IDVNLPRVKAKSVDVAEEKLVLTVNETREIFLNKTKMTLGELKPKLEAIFSNRIDREI
                     FLRADKNVPYGFVVEVMSEVRKAGVDKLGMITEPPEETK"
     gene            5436..6155
                     /locus_tag="PLG98_04820"
     CDS             5436..6155
                     /locus_tag="PLG98_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOE32607.1"
                     /translation="MTPRMLGKGYRSGNGNFQNMIFLSLTLHVVVVALILVSVPTTSR
                     HLTFGAAYSVQLVGSEAVMPSQETSSLSDILESKKTSDAIIIKRKISSIYSSPLKTPA
                     TQKLDVEKAVGALRQKERAPGKTKENAPASGRTAMTDAEVNAQANEYIETVWSRVKQN
                     WSIPQSLLPDNNITTIVDVRIARNGALLYAGFEKRSGNRYFDDSALRAVKKSGPFPPL
                     PHWIRDNHIEIGIRFHSSELR"
     gene            6207..7529
                     /gene="tolB"
                     /locus_tag="PLG98_04825"
     CDS             6207..7529
                     /gene="tolB"
                     /locus_tag="PLG98_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOE32608.1"
                     /translation="MTKRVLILTLIFFFCAWPICSNRADSKVYVDIDSPAFRLIPIAI
                     CDFHPASGPVGHVASETTVSERVKKDLALTGLFNILNKQSFLESPDAVSTTAVGKIRF
                     ADWLAVGADYLVKGRIFQKDREMTVEAYLYDVARGELIFGKKYQAPAEKTKAVARAIA
                     SDIMLALINDAGDFNTEIAFVSKTGLRSDIYAVSYDGADVRKVTHHQSILMAPRWSPD
                     GNGMAFTSFKRGRPEIYFLNLKNRSEKRLASFGGLNLCGSFSPDGRKLLMTLSKDGNE
                     EIYALDVQTLQLQRLTRHSAIDVSPSWSPDGKQIVFVSNRGGSPQIYTMDADGNNVKR
                     ITYKGSYNSSPSWSPRGDRIIYEGLTADRYQIFSVDTEGNNPAQLTSDNANNESPFWS
                     PSGRQIVYVSRKHSGSQILIMNANGTNPRLLYEQSNRLAMPAWSGRLR"
     gene            7729..8298
                     /gene="pal"
                     /locus_tag="PLG98_04830"
     CDS             7729..8298
                     /gene="pal"
                     /locus_tag="PLG98_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOE32609.1"
                     /translation="MNMQIHLRLVLVVFIAGLMMIAGCAEKKSVITSDAAQEQQTTPA
                     QTTPSQEDMSSKDITGSQIASEEKSVDLEKMARAQSPVNDIHFDFDSSSIRPDARETL
                     RENADYFMNNRISSIIIEGHCDEKGTNEYNMALGERRAQETKKYLVNLGVKESLMQTV
                     SFGEENPLDPASNEDAWAKNRRAHFAVNP"
     gene            8320..9132
                     /gene="ybgF"
                     /locus_tag="PLG98_04835"
     CDS             8320..9132
                     /gene="ybgF"
                     /locus_tag="PLG98_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOE32610.1"
                     /translation="MERFRYFILFLVVLFLAGCASSGDLRALRSEMSQRTDEKISSLE
                     AEVNALKENATALESMRKGQANTMADLAELRENLQQLRGQVETLKKDASQSSRKDEQL
                     LNNMLLRINFIENFLEIGSKNTSDASDKTGKVNGGSVAKDTGSKQDKDKAYSAAYQIF
                     QEGNYAKARNEFQTFLTTYPNSEYSDNAQFWIGECYFFEKKYESAILEYEKVTKNYPK
                     GNKVPYALLKQGLSFLKLGDKTSAKLLLQEVIKNYPNTSQARIARSNLQDIK"
     gene            complement(9157..9894)
                     /locus_tag="PLG98_04840"
     CDS             complement(9157..9894)
                     /locus_tag="PLG98_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOE32611.1"
                     /translation="MLCLCSCALQQASGVARDVYPEKRILSTSGGLAEEDVFQALAKI
                     DFKTVDASYHVRAALVLRRPGYVRLEVLPLIGNPDLLLTATPDTMTVFVPSKKELYRG
                     RPTRSNLEKFLPWSMDIEDMVLVLTGTYPFCTTERLAEDVSSEGRLWRMTCKKSSSES
                     QIIWVQEGSQKLMRLLCTDETGSEKYNVQYFYGGQSALPEKLVIRLANGSTALTVTYS
                     DVRVEKTKDLSVFNLTAPSDVEQNILE"
     gene            complement(9970..11658)
                     /locus_tag="PLG98_04845"
     CDS             complement(9970..11658)
                     /locus_tag="PLG98_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32612.1"
                     /translation="MKRNFICFVVIMFVSLSGCVTLPNVQHAPSGDKFQQQASYHYLL
                     SVFLRNDGNLDEAIRELEQAYAADPDSAYLVIELMSLYAQKGDVKSAVAHGEEALARD
                     PDNVDVHLIMGGLYLNMREYSKAVREHKKVIELDPKNLVAHLYLGIIYAEEKRFDRAE
                     ETFKKMLKIDPDNIMGVYYYGKVLEKMKRLDEAEKMFKKAMSLNPYFEMALFDLAALY
                     EKQEKLDKAIEIYRNYLAIDPTRLNLRVRTGELLIKKKKYEEAEKEFQEVLKIDPDNR
                     DIKVALGLLYFDMKKFDLAAAEFSSILQKNVLDDKVRYLLATTYDQQKNNVRAVEEFT
                     GIASSSELYVSAQLRAAMILKEEGKVTEAVAVIKKSIKKKNDQVPFYLLLSSFYDETG
                     DNASSEAILQDGLKVLPRSTDLHYALGVLYDKTNRFEESIMSMKKILDIDPKNAEALN
                     FIGYSYAVRDMHLDEAEAMILRALKIKPGNGYLLDSLGWVYFKKNDLKNAEKYLKEAW
                     TLLPDEIEIIEHLGDLYVRQNRLKDAADMYERGMKIDPGNDVLKKKREDIRGTR"
     gene            complement(11708..12574)
                     /locus_tag="PLG98_04850"
     CDS             complement(11708..12574)
                     /locus_tag="PLG98_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOE32613.1"
                     /translation="MKKIFTATLVVLILLSVAGIIYVVRFQEAVPEKAKEKEVAVKPA
                     APKIDSVKKRTSPPARLREVAIIIDDIGYDLNVAKALLKIDADLTFAILPFQRHSREA
                     AEMIYQANREVLVHLPMEPVSYPRERPGEGALFTDMSQEELVLQLRKNIDAVPHAVGV
                     NNHMGSKFMMDDEKLSVIFKELQRKKLFFIDSRTSPDTRAYAIADDMGFRIAERKIFI
                     DHHRDYHAIYSQLMHVAQSNDPAPKIIIGHPYPETVRALQAATKVMREKGVSVVPASR
                     IIKKTNKSPEKS"
     gene            complement(12611..>13271)
                     /locus_tag="PLG98_04855"
     CDS             complement(12611..>13271)
                     /locus_tag="PLG98_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOE32614.1"
                     /translation="ELDDKNKPMKGLILDLRNDPGGLLTQAIDVSDIFLKSGTIVSTR
                     GRVKAMETKSVAKNDNNEISCPIVVLVNEGTASAAEIVAGALQDNGRALIVGTQTFGK
                     ASVQTVIPLEDGSALKLTTARYYTPNGRSIQAEGITPDIIVPFIKGAEEAESVTDKEE
                     RIREKDLKGHIKPDKDDGTKVDGGQSKDTKDPLDLEKDNQLQTAVNILKSWNIMKSIK
                     K"
     assembly_gap    13272..13371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13372..13745)
                     /locus_tag="PLG98_04860"
     CDS             complement(<13372..13745)
                     /locus_tag="PLG98_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32615.1"
                     /translation="MMKKLSIKFYLLAVIGVGALILLGLSSDNSAAAKDKNFYKDIKT
                     FSEVLDMVRKNYVDDVDTTNLMQGAINGMIRSLDPHSSFMTPDLYKELEVETQGQFGG
                     VGIEIMLIKDILTVVSPIEDTPA"
     gene            complement(13867..15348)
                     /gene="cysS"
                     /locus_tag="PLG98_04865"
     CDS             complement(13867..15348)
                     /gene="cysS"
                     /locus_tag="PLG98_04865"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOE32616.1"
                     /translation="MPQKIFNTATRKKEVFQPIQEGAVGIYVCGITAYDVCHIGHARA
                     AVVFDVVVRYLRAQGYNVTYVKNFTDIDDKIIDRANREGVSIADIAERYIKAHDEDMA
                     ALGVRTPTITPRATENMQEMIALIRTLEDKGYAYCVEGDVYFSISDYKQYGGLSGRNL
                     EEMMAGARVDVSDKKKNPLDFALWKNSKEGEPFWESPWGKGRPGWHIECSAMSRRYLG
                     ETFDIHGGGEDLIFPHHENEIAQSAAATGKPLARYWMHNGFVKINSEKMSKSLGNIFP
                     VKEILKHYHPEVLRLFMLQSHYRSPVDYSDDSLSEARAALFRCYRTLQQMKELQGQLA
                     AGSKPVATQPSGRVDDYVNAFQMLKDKFNEAMDDDFNTAQALGYVFDMVRQMNNFMMK
                     EKSMPVADKTAVLAAAIRVFGYFGSVLGVINSDADQFFVLYRETELRRRELNADEIEE
                     LIRQRQSAREAKDWARADDIRKKLAGMNVVLKDSADGTSWTIE"
     gene            complement(15367..16404)
                     /gene="argC"
                     /locus_tag="PLG98_04870"
     CDS             complement(15367..16404)
                     /gene="argC"
                     /locus_tag="PLG98_04870"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOE32617.1"
                     /translation="MIKAAIFGASGYTGQELIRILSGHPDVKLVAATSRRFAGQEVST
                     VFPALQGLTALKYQNAKPGEICRLCDVVFLALPHGVSMDMAPEFINAGKTVIDLSADY
                     RLRDLKIYEVWYGKHASAKFIKNAVYGIPELYRQKIKKTKFVANPGCYPTGIILGLAP
                     ALKKQLLDVSTIIADSASGVSGAGREPFAGSLFCEVDGGFKAYKVGKHRHLPEIEQEL
                     NALAGKKFAVSFTPHLLPVKRGILSTIYAKLKKDIALGDLHALYSSFYAGETFVRMCP
                     EGVYPNISSVCGSNYCDIGLAVDPRTKRVIIMAAIDNLVKGAAGQAVQNMNIMCGFKE
                     DTGLQIAPLFP"
     gene            complement(16452..16844)
                     /gene="rpsI"
                     /locus_tag="PLG98_04875"
     CDS             complement(16452..16844)
                     /gene="rpsI"
                     /locus_tag="PLG98_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOE32618.1"
                     /translation="MAEQRFYATGKRKSAIARVYMKAGNGNIVVNKRSYEEYFPRPSL
                     KMLIKQPLDITGKKDQFDLYINVTGGGVAGQAGAVKHGISKALLEYDAELRSVLKKAG
                     FLTRDARVKERKKYGQPGARKRFQFSKR"
     gene            complement(16886..17317)
                     /gene="rplM"
                     /locus_tag="PLG98_04880"
     CDS             complement(16886..17317)
                     /gene="rplM"
                     /locus_tag="PLG98_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE32619.1"
                     /translation="MMKTYMAKAQTADRKWYIADASGKVLGRFASEIAHRLRGKHKAT
                     YTPHVDTGDFIVVVNAEKIVLTGKKMTDKIYYSYSGYPGGLRETTAGKMLAEKPEDLI
                     RIAVQGMLPKTNLGRRMLKKLKVYSGNAHIHEAQCPKTLSI"
     gene            complement(17842..18591)
                     /locus_tag="PLG98_04885"
     CDS             complement(17842..18591)
                     /locus_tag="PLG98_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE32620.1"
                     /translation="MANQQVPEKVVRILMRDIFNNKLKPGTKLPSAKEISRQMKVDLS
                     SLRIGLKQLESMNVLDIKQGDGIYVKDYVQHAGIDFLSLLFSQKDENEQKMIVDDYFV
                     DEIWEFWTAVFPEILKMAANRFSPRDVKAIAGLFNEELASLDDREKVIELQEKQQDLV
                     VRVANNTIFLLLANSTRPMRRKIIELFVNSIDRKTLETFAKTKVRLLKEYTSGTSTNA
                     LGASEKYREMLFLTRQAVKTALAQQAKTPSP"
     gene            18786..19577
                     /locus_tag="PLG98_04890"
     CDS             18786..19577
                     /locus_tag="PLG98_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOE32621.1"
                     /translation="MIVAGCDVGSLTAKALIMNEKGRLAGEITRVRLSTLDSATEVME
                     KALASAKLRFSDLDYCCSTGYGRYEIPFAQMNMSEISCHGLGAFRADRSVRTIIDIGG
                     QDCKIILIDAEGNVRDFIMNDKCAAGTGRSLEMLAKTIGVDLETLGSLAVKSKNPISI
                     TNKCSIFMELEVLNHLYARKKLKHIACGIADAVAKRVAALAKAIDVEKNICITGGVSK
                     NPGVVRQLAYHMNVSFTSLPVDPQIIGAMGAAVYAMETLKAKVGM"
     gene            19574..20389
                     /locus_tag="PLG98_04895"
     CDS             19574..20389
                     /locus_tag="PLG98_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOE32622.1"
                     /translation="MITAGVDVGTRFIKVCVTDGEHLRGSACAEIGPRFDALIRSLLK
                     EARVQARAGRWEIKKIVATGFGGHLVKKAAFTLGEAACAARSAFFFDRDIRTVIDLGG
                     LFITVSTIDKNGFLETTHVNERCAAGSGKFLEMVAEAAEVPFTEISSCAAASRQPYSS
                     SSSCAVFAESEIISQVNTGMKPSDILAGVIRAIASKTASLIDGAGAKDKIVLIGGLSK
                     VSAVRDMLTGLTGRSMTTLPIDSQLVAAFGAALIAQGKIIRKRQGLSVTGEAA"
     gene            20389..21135
                     /locus_tag="PLG98_04900"
     CDS             20389..21135
                     /locus_tag="PLG98_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32623.1"
                     /translation="MELTDCTPLMKQVTEMVRDQRRGFSRHLSDHGEEARRIGFLSRD
                     KKPDRAVIVTAEDTALELGSPRHPSVSTFLWTKQKDLLSRAIWTAGRDFPQIKESPVS
                     FAQVIMVELEGDFDPTGPNIQTLSNLTNRIPGYMTRSIPGKIWIRIHRDLLARKFSLQ
                     SLGQCLIQAYHESIPGLKNIEVALIADNADFVGHLIPIHNRARIIYGENKKLVLEADG
                     TISCEDLDCENCTEKPTCDTLRDVIVKRRT"
     gene            21138..21932
                     /locus_tag="PLG98_04905"
     CDS             21138..21932
                     /locus_tag="PLG98_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE32624.1"
                     /translation="MNEPIILAFLGKGGVGKTVLSALTGTILSACGKKVLFVDADPAM
                     GLTSALEVSDIKTIGAAREEIIRQARISSSSEEKEKLADIVDYLLMEALYEGQGFGMV
                     AMGQTNTLGCYCPVNNLLRSTIKSIASQYEAVIIDAEAGIEQVNRQVVESVHYPVIVT
                     DNSLRGARTAVMISSLLKKIPQLSPVKVGTVFNRVDQADENLVKMILEAGMDYYGSLR
                     PDNAISALDRQGLSIAKIAGTSPAAAGMRSVLEQIGAFGKILNVKK"
     gene            21992..23395
                     /locus_tag="PLG98_04910"
     CDS             21992..23395
                     /locus_tag="PLG98_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOE32625.1"
                     /translation="MRDVYVVAAHTIKFGKYLEKTISELSAMTVSPCLKEAGLDKKDI
                     QALWFSNSAWGEYKRQVCIRGQVALRPLGIDAIPITNLENACAGGSTAFHHAWMGVAS
                     GMYDITMALGAEKIYDQNQALVFSGFLTGLDVGNFGKTLERLGDVALSENEKKAMATH
                     IEKYKGLAKQSSGKGRKKSFKDKFMAQRDALVVAIRIGETMGYDMLKKLRKLGSGDHS
                     PFMDVYGYEARQHMKKFGTTIEHLAAIASKNHFHSSLNPNAQYQFLVSTEQVLADRLV
                     TWPLTRSMCAPIGDGAASAILCDEATVRRLGLSGQAVKVRASILGSGMARDRDGIDIG
                     ERLSRLAYEKAGVGPAEIAFAEVHDATAYGELHQCESLGFCRLGEGGRYAESGATKLG
                     GKQPVNPSGGLESRGHPIGASGLAQIHELVTQLRGAAGPRQVSGAKLAIAENGGGALG
                     VEEAAMCIHILEAPSLK"
     gene            23418..25118
                     /locus_tag="PLG98_04915"
     CDS             23418..25118
                     /locus_tag="PLG98_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE32626.1"
                     /translation="MNRKLRKLDLGKNILKSICLFSINEIRRGSFLKNIRGVSRTEIR
                     QEMSWGELLEEKAARYKDKIFLIFEDKTFTYRQMNANANRIANYFLSSGAGQGKGVAI
                     LMDNSPSFIDIFIGIQKIGMYAVPVNTSLKGDSLLYILNHCDAEYLVIDETHLETLNK
                     IADRLDKIKTIIINPSPSSSRQYPAGYHVLDEAYKGSPANPGLGYRKDDICFITYTSG
                     TTGLPKGVVYRYRKSSVKLLSLVAVMLFKKSDVLYTGMPLFHGNALFVTVTCALHAGA
                     RVVLARKFSASRFWDDIRKYNVTTFNTIGAMIPILMKQPEKPSDRQHNVRFIISAACP
                     ADLWEKFEKRFGLTIYETYGAVDGGGKIILNLGTAPVGSIGKPPMKVKYRLVDANMKD
                     VPDGTPGELVFPVSNKKSTSSVEYYKNEKASAEKMNGDYIFTGDLIRRDTEGFLYFIG
                     RNTESMRIKGENVSAFEVEQAIQKHSNVLEAAVYAVPSELAEDDIMASITLVEGMTLK
                     ETELIEYLKENLPKFAVPRYVKIVPELPKTETHRVKKKELEALKVVTGTWDENKKAYV
                     "
     gene            25183..25632
                     /locus_tag="PLG98_04920"
     CDS             25183..25632
                     /locus_tag="PLG98_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32627.1"
                     /translation="MSKINTGFWQKMFFVGSLWNLGIGITSLLFTDFMLMMMFGKGPI
                     EDNLLAFINGTVPVTDNLQTLIFFRFFMIAVILFGIGYYWVSRDLLANRAVIWLGLVA
                     KLIIFFTFVYYYALEQAAWFPVFVLSGDFVFSIFFVAFLWKTKDGIY"
     gene            25667..26416
                     /locus_tag="PLG98_04925"
     CDS             25667..26416
                     /locus_tag="PLG98_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOE32628.1"
                     /translation="MAKQTTDNLSPLYTYQEGMEWNPVEKVIMERRSIRNFKKEPVPD
                     NLIRRVLEAARFAPTAGNAQPWKFIVVKDQALIAEMERATIQLSKLLMWFVDYNRNAF
                     RRIFLAPYTKFMTRLMPNKLHPIPFNLLQQIAAEKTAVYYNAPVMILLLIDKRGIGNP
                     VLDAGICGQNIVLAAHSLGLGTCWIGMMTVLMMNPLWRRKLGVKSPYELTDCIVLGWP
                     KGDYDGEVTREVQLVEWLEAGGEKRIERQGV"
     gene            26418..26768
                     /locus_tag="PLG98_04930"
     CDS             26418..26768
                     /locus_tag="PLG98_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE32629.1"
                     /translation="MLDLSLGKKGKIPNYFKETQMTYGVVVFDPTLCTGCGICVSICP
                     ARGLMLTKRQDSAKKKIPLVIETAPGISLCMACGDCTAACPEAAIRIKRGFNAGFFFR
                     KLSQQPELTLPKKY"
     gene            26791..28182
                     /locus_tag="PLG98_04935"
     CDS             26791..28182
                     /locus_tag="PLG98_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOE32630.1"
                     /translation="MKNNPKPFQYLKWSSSFLNFGYYLTTFPNLSYWALRLTRPLYVK
                     RETFFNHKFRYAFLILGGGILPPEEIKAYHYGAKAFVDNMKNALKANENGKPVVWVEW
                     ILSSEIVNAFDAFPFNPETLNVFGNLSGQDVPSLLIEEAESAGFASEYCSAIKLTAGA
                     YMLGQTPKPAAIIAGSHPCDTNVAAYQGIRHLTGAPVFFFDSPYWRDEDAFDYYEKNM
                     WRLIEFLEKTLHQKINWERLKQIVDHENKFNFYMQEICEMMRAIPSPATMITPVFAWV
                     VRELAIGDPNGTEMARRMYEVVKDRFKKGVGVVKKENIRVLWWNPPIAFFAYVFKWME
                     DEFGAVVVKDFIGDVQMPQIDTSSEKTMIRDMAKNHLYLAMARQCHGPVEFITQELEK
                     AIDEYSPDCLIFAGHAGCKHGWAVIGIIKDLLKKRGLPSLFMNMDIMDQRHATEDDIK
                     RWITEFFRSNGFV"
     gene            28179..29369
                     /locus_tag="PLG98_04940"
     CDS             28179..29369
                     /locus_tag="PLG98_04940"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOE32631.1"
                     /translation="MKTIIDETRDLALSTHNHYLEEARKQGKKIIGYFCSYMPEEIIH
                     AAGFVPYRMRAVESKGTTRADAFYSSINCTFVKHCFEKALKGDFAFLDGVVFVNGCDH
                     SRRMYDNWRFAKIDPPFLHMFFAPHMINEAALDYFSLECSKLKSSIEKHFHVSINDES
                     LRRSINLYNQKRRLLAQIYALRKMKDVPIKASELLGVMLAVTAVPVERAIDILSNVKN
                     YITGRVVSKPQDLRLFFSGGCIEEMDHLELIENCGAVIVADNICLGSRHFLDEVKNDG
                     IPLQDIADRYLSHLSCPRMMGDFERRFNYLLDVRDEYGIDGIIIEKLKFCDIWGGEIY
                     LHRTESKKRGLALLALERELYGGGIGQIKTRVQAFLEKLRNKQATDENLVQAAGADYK
                     PKIQ"
     gene            29388..30758
                     /locus_tag="PLG98_04945"
     CDS             29388..30758
                     /locus_tag="PLG98_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOE32632.1"
                     /translation="MKKKGSGYEYIIVGTGPGGAPAARELAKAGKKVLMVEKGDYHKK
                     FLGLPFGIRLLERYMLFARSREGVIIERGITVGGSSMVYQSNVMDPPVKLINNMGVDF
                     RPEAKQLKKEIGIEVLPEKFYRNNKGLYRMLATADKMGIPFREQEKFVHADKCKLGCD
                     WCMLGCPENARWTTREHVEEAKSYGAELIFNAPVNKILFNAGKTKATGIQLKNGTVIH
                     GDNIILSAGGIGSPAVLLRSGVKRLGTQDVGTRFFMDPMNIMLGYSKDPDGGLWGIQT
                     FSHAIESMAQSEGFMIGNCSAFGTWTVLSFFRMQTARENLPKFPIMKRGMGLFLKLSD
                     EAHGQIFPNEKTSKPFTANDEKRLKKGTDIMREIIIKSGGDPKTISILKWAGGHPGGT
                     LAMGQAVNRDFSTEFENLYVCDGSTMPVSPGAPPSLSICSMSLLLGKMLLGKVKAQDR
                     LIKAAG"
     gene            complement(30925..>31761)
                     /locus_tag="PLG98_04950"
     CDS             complement(30925..>31761)
                     /locus_tag="PLG98_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE32633.1"
                     /translation="PYTKAVIPVHMCGSAANIDKIVDVARKNKLLVLEDNAQGCGASY
                     KGKKLGSFGDMGTFSFDYVKTVTTGEGGMVITNNKEFYLRSEWYHDHGHDHNPKVSRA
                     LEGRTILGFNFRMNELQGALGLAQLRKLDYLIGEQKKNKKFIMDVLANVSGVKFRVKP
                     DPEGDSATFLAFNLPSEDQAQKFQNLLAAEGVDTVCYKRNLWHYVPNWEHFLAKSTAN
                     SKKYPLTNPAYKGKVEYRKENIPQAEDILGRTLVMGISVKMSQEKLDGIRKGIEQAAK
                     NM"
     assembly_gap    31762..31861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(31932..33089)
                     /locus_tag="PLG98_04955"
     CDS             complement(31932..33089)
                     /locus_tag="PLG98_04955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE32634.1"
                     /translation="MRKTFSFTGAKKIVFGSGSLLTLASHVKEHHARNPLVVIDKNLA
                     KTDLLEKVANVLIPSGIKFTVYEKVEAEPPIELADEGAKAAMKNKCDIVIGIGGGSAM
                     DVAKAIAVIATNKGAAADYLGLNKVPKPGLPKIMIPTTAGTGSEVTFTAVFLRKNLKK
                     KEGMNSPYLYPELALLDPELTLSLPPVPTAHTGLDALCHAIESYTSINSSPVSEMFSL
                     EAIALIAENLRTCVHDGKNIVARERMLLGSLYAGIGLANAGVTAVHSLSYPLGGKYGV
                     GHGLANTMLLPAVMAFNMPSALEKFTDIAEAMGENVEGLPEREAAYLALEAVEALIED
                     CGIHDTISTFGVKEKDFPALADVAVTVARPLENNPRKLTKDDMIAIYTQAF"
     gene            complement(33225..33965)
                     /locus_tag="PLG98_04960"
     CDS             complement(33225..33965)
                     /locus_tag="PLG98_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE32635.1"
                     /translation="MLKTQLKNISVVLYKPKYAGNIGAVARAAKNMGIRKMIVVGTDD
                     FDREEMRQRSTHLAADVLDDIQYIENAAEAVSPFNYIVGTTARTGKARGPFVTPRQAA
                     QTIADISQKNKVALLFGPEDTGLANEQLRLCHMVVTIPTSREFTSLNLSHAVMILCYE
                     IFTASCIAVTASEATPKLALSSELEGMYGQIKTLLADIGFLNPENPDYWMMHLRRFFT
                     RAGILSREVKIVRGICRQLQWYAHHKKT"
     gene            34237..35154
                     /locus_tag="PLG98_04965"
     CDS             34237..35154
                     /locus_tag="PLG98_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family murein peptide amidase A"
                     /protein_id="HOE32636.1"
                     /translation="MVKVKISLISVGLVLLFAFFLLLPLQSFAYDCSTREGKQYLRLL
                     ERKFADYRWTDLHPRDISWEYHRTTRNKHPLMFVHFGNSSKNCVLFFGGVHGDELPTA
                     YLMFKLAHYVKEHPALFKDQCIVIAPLLNPDGFLSAQPTRTNASGIDINRNFPTRDWK
                     AKAIRQWIAKGKQVRYYPGTRPASEQETIFQMALIKRFKPQKILSAHSPLNFFDYDGP
                     SSDLDSFEKWMEQICKESNHPLKKFGYYPGSLGNYAGHERNIFTLTLELPSSDPGQSS
                     DYFEKFQPSIVKFIQLPIVGSPPNLKVLR"
     gene            complement(35255..35620)
                     /locus_tag="PLG98_04970"
     CDS             complement(35255..35620)
                     /locus_tag="PLG98_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32637.1"
                     /translation="MIKKNLGQYLAQKKFIRKILDDPVDLSEFKQRPTPRLIGGLVLM
                     ALSYVIGWPIIFVLSILAVSFHEPLIFVIGGPLAYAVSCVVFLIGAWMARAPHYMNTL
                     TRYALQSLLKKLFDPVRTD"
     gene            35791..36507
                     /locus_tag="PLG98_04975"
     CDS             35791..36507
                     /locus_tag="PLG98_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32638.1"
                     /translation="MNSSTNGWDFFDRFYCISLRKREDRRQSARKEFEKVGLANRVEF
                     VIGERLSDDFEREVYEAHMLCLRKGLEAGAKNIVVFEDDVEFDRFDPERLQKCTDFLR
                     RHPGWKVLLLGALIRSSRNTDDPCVQKVRYQSLTHAYALNAPYAKTLAYDPWSGIVND
                     TLFRPLTEDVYAVCPMCAFQKNFTSDNYKYPRLALIRRLFGGLKRIQKANEFYQRYKV
                     GVIAAHLAVVILILFLLWRG"
BASE COUNT         9311 a         9097 c         8764 g         9224 t
ORIGIN      
        1 cgggcggcgg aagatcccct tcaggaagat ttgcatatgc cggttcccaa actggtgcac
       61 cgttatccgg accgttgtct ggcgattgtc acggaaacct gcgcggtgta ctgccggcac
      121 tgtaaccgaa agcgtttctg gtcccgcccc gcttccctga acctgactga ccgactgcaa
      181 aagataaccc gttacattgc cgaatcgccc cgcatccggg aagtgattat ttccggcggc
      241 gatcccctga ccctggatga ccgtgttctc gaaaaaatcc ttgcttctct gaaagccatc
      301 ccgcatgttg acgttctgcg tatcggcagc cgcgtgcctg ttgttctgcc gatgcgcatc
      361 acccggacgc tctgccggat gctgaaacga taccggcccc tgtggttcaa tacgcagttc
      421 aatcaccccg gggaaattac accggaggcg gcgcgggcct gtaatatgct acaggaggcg
      481 ggcattcccg tctccaacca gtcggttctg ctcaaaggcg tcaacgattc cccggacatc
      541 atgggtgatc ttctttacgg tctgcaaaaa atgtccgtcc gcccctatta tcttttccat
      601 tgcgacccgg cacaagggtg tctgcatttt cgcaccgagg taccggccgg ccttgatatg
      661 atggagaaaa tatggcggca ctgttccgga ctttgcgtgc cgcaatatgt tctggatgtc
      721 cccggcaagg tcggaaaaat ccccttaaac atcctgtccg aaacgatgaa aaatgattta
      781 aagaaacatc aacatttttt tgacaagcgt caagaaatag attagagaag cgcctgaggc
      841 aaaacaggcg attttgccac actaagcaag gagaaaattt atgtacactg ccagtgattt
      901 aagaaaagga ctgcggctga aaatcgatgg cgaaccttat gtcatcgttg aattcaattt
      961 cgtcaagccc ggtaaaggac aggccctgta tcgcaccaaa ctcaaaaaca tgatcaacgg
     1021 caatcagttt gaacgaacat ttcgttctgt agatacattc gaacaggcgg atttacggga
     1081 aaagaccatg cagtatctat ataaggaagg agataaattc tgcttcatgg acaatgaaac
     1141 cttcgagcag gtttatctga cggaagaaca ggtcgatgat gccgtaaatt ttatgacgga
     1201 taacatcgaa gtggaaattc ttctttttga agaccggccc atcggcatca gcctgcccaa
     1261 ctttgtggaa ctggtcgtca ccgaagccga accctgggcc aaaggtgaca ctgccgccgg
     1321 ttcaaccaaa ccggtcaagg tacagacagg ctatacgatt caggttccga ccttcgtaac
     1381 cgaaggtgag aaaatcagaa ttgacacaag aacgggtgag tacctgacta gagtcaaagg
     1441 ttaacgcagc ttttcaaaat gcttatctgc tgcgttgcgc ttcagcctgc gtccttgcgt
     1501 cgtatcataa tacgcttcac tccgcatcct tcgtgcgcct tgcctctgag acaatttgaa
     1561 aaagcttaat caatatgaac gacttttttc aagataatat ctttcatccg gcgcgcaagt
     1621 ctcaggcctt ggagatgcga gccaaactca ttcaaggcat ccgtcatttt ttcatcaaca
     1681 gcagctttct ggaagtggac acgccggtca ggatcccctc gcccgcgccg gaagaacata
     1741 tcgaggccct ccgttcggag gattggtttt tgcagacatc gccggagctc tgcatgaaga
     1801 ggttgctggc cgcaggctac ccgcgtattt ttcaaatctg caaatgtttc cgcgccggcg
     1861 agagaggcga tctgcatctg ccggagttta cgatgctgga gtggtatgtc gccggattcg
     1921 attaccggca actcatggcc caatgccgga acctgatcgt tgcggttgca caaaatcagg
     1981 gccttactga aatcaaaaga gacaaccgga ccattgcctt aacaccgccg tgggagagca
     2041 tcaccgtgga agacgccttt ttaagatacg cgccgcttac cctggatgag gctctggggc
     2101 agggcacgtt cgatgaaatc cttgttgact gtatcgagcc ccggctggga atcggcaaac
     2161 cgacgtttct ttatgactat ccggcctcac tggcggcgct ggcgaaactt aaagacaatc
     2221 atcgcaccgt cagcgaaagg ttcgagctat acatcggcgg gctggaaatt gccaacggct
     2281 tttcagaact gaccgatgcg gcggaacaaa gaaaacgttt tgaagaggcg ctgaaaaaac
     2341 gggctgaaaa aaaatggtcg gagtatctga tgccggagaa atttcttcag tccctgggac
     2401 acctgccgcc ctgtgccggc attgcgctgg gcattgaccg gctggtgatg atcataaccg
     2461 gtgcaaactg cattgatgat gtggttgcgt ttataccgga aaatctttga tccatcgttg
     2521 ttaagaaatt aatgcataaa aaaacccgcc ccccgaataa ggggcgggtt tttgtttatc
     2581 tggtttttag tgattaatgg gtgatgacat tcagtgtata gaaagtgcct tctgtcaact
     2641 ccattgtttc cgtaccaacc atgaacgtac cgtcaaatgc cagctgtcca tggaaaacaa
     2701 ttgcatcggt ttcaggattc gtgacgattg cctgaccgtt gggagcaatg gtcagtttct
     2761 tgacactgac cagacccaac ttctgtatcg gtgtataagc cagagacaaa ttccagttgt
     2821 aggacagcat gacatccaaa ttgaaggtaa gcgccaatat atccggcagg aaggacgtct
     2881 caaaccggct tacattaata tcatgatgcg cccagaagac gtcatcgccg acacccagca
     2941 gatggttata ggacgatccg gtcaaattgg ccacagcctg tccatcggta atctgaacga
     3001 tcattaactg ataatcgcca ttaatatcgg tataggttcc aaccattgtc tttttatcga
     3061 cggagagaaa tccttcgaaa tcactggctc ttgcccctaa agcatccacc agggtcacaa
     3121 caccgtcaac cgctacggct aacgttacgc cattgatggt tgcagaagga gcacctgtgg
     3181 gacctacttc ttcgctcatc gttgttacca gagcggtatc ggttgctcct gttccatacc
     3241 accactcatt ggtttgaccg acaaccatgc catgaacgac gaagttatga cttgccagat
     3301 tggcattgct gtaattggtg gtcccttctt tctgcgcgat catgatctgg tagtttgttc
     3361 ctgcggttgc cgtgccggca gcaaaatcca tatcggagtt catggtcatg tgaccgccgt
     3421 ctcccgcagc ataggcaccg ctttgaatga tcgcacccgt atctggattg acggtaaatg
     3481 ttaaatcaaa atcggccgga caggttgtat cgcccgctga cgtgctggtt tcatacgact
     3541 cacacgtggc aacaccggtg gcgccgatgg tcaagcgggc acgcacccac ccattttcgg
     3601 tgcccgttcg aagcatatta gtacgccacg ttccggccag gtcggcctga ccgtatacgg
     3661 atgcttccgc agccgtgaag ttcacgcctg ttatatcggt caaacctaca tcgaaagttc
     3721 ttctggcggg ggtgaaggtg taaccctttt taccgggtgt aagaataacg ttatactgtt
     3781 cgacgagcgc tgcgggcagc ctcaatgtgt aattgccgtt ggcatccgtg ataatatagc
     3841 tgaacaaatc actgctgatc gagccctccc agaccttgac attcaggatc gtcaatgagt
     3901 acatggcgga aatattgaca ccctgaaccc ctgcgccggt cgcgtcagtg accgtaccgg
     3961 aaacagtcac aaataactgc tgctgatttt gatcatcatc gtcgtcatcg ctgccgccgc
     4021 tgctgcaacc ggcgactatc aaaaacatca acatcagaaa atataacaaa aattgagtta
     4081 aaattctttt tcttttcata ggtccccctt attattatta cgttttaatc aaatagtttt
     4141 taagttatcg gtacatctac tatcatttct aaaaaaaaac aagaagtttt ttacgttaaa
     4201 agatatattg acaaggactt ttttttcctt tagcttacgc aacaaattca tttcccattg
     4261 gagattaaaa gtgaaagact ttggagcttt agacttaagc ggcagtgtga acggcagcct
     4321 tttgagcatg attctcgatg ccgggctgat ggttaagttt gttttgctgg tgcttttcat
     4381 tttttctgtg gtttcctggg cgatcatttt catcaagtat cgctattatc gcaaaattca
     4441 aaaagaaaat gaagcgttta atgacgacta tctgaagagt tcgaaacttt cggatgtctt
     4501 gccggcagcg aaaaaatatt catactccac cacggcggaa gtttttcgcg tggggtacaa
     4561 ggaactgacc cagaccaaca gaccggccgc aggtgaatcg gcccagggac gcgacgacat
     4621 cagtctttcg tcactggata atctcgaacg ctcgatgaat aaagccagtg ccacagaaat
     4681 gaccaagctg gaaagttcgc tgggatttct ggctacaacc gggtcggcca gcccttttat
     4741 cggtctgttc ggcaccgtct ggggaattat ggatacgttt aaaggcatcg gcgcgcgcgg
     4801 gtcggccacg ctggctgttg tcgctcccgg aatttccgaa gcattaatcg ccaccgcagc
     4861 cggactggct gcagcgatac cagcggttat cttctataat tattttttaa atcagtcgaa
     4921 aaacatggtt caggaaatgg aaaacttttc cgctgaattc ttaaacattg tggaacgata
     4981 tcttgtccgt aaataaatcc cggagacaaa acaatggtgc gcgggtcacg cagaaaaaat
     5041 aatgccagta aaccgctggc ggaaatcaac gttacgcctc tggtcgatgt catgctggtg
     5101 ctgctgatta tcttcatggt gacggcgccc atgctgtcga tgggcatcga tgtcaatctg
     5161 ccgcgggtta aagccaaaag cgtcgatgtg gccgaagaaa aactggttct gaccgtcaat
     5221 gaaacccggg aaatctttct gaataaaacc aaaatgacgc tgggggaatt aaaacccaaa
     5281 ctggaagcca tcttttccaa cagaatcgac cgggaaattt ttctgcgcgc cgacaaaaat
     5341 gtgccttatg gctttgtggt tgaagtcatg tcggaagttc gtaaggcagg ggttgataaa
     5401 ctgggaatga tcacggaacc tcccgaggaa acaaaatgac accccggatg cttggcaagg
     5461 gctaccgcag cggtaacggc aactttcaaa acatgatctt cctttccctg acccttcatg
     5521 tggttgttgt tgccctcatt ctcgtgtcgg tcccgacaac atcgcggcac ctgaccttcg
     5581 gagcagccta ttccgttcaa cttgtgggct ctgaagcggt tatgccttct caggaaacat
     5641 cttcactcag tgacatcctg gaatcgaaaa aaacctccga cgccatcatc attaaacgga
     5701 agatcagcag catttattcg tccccgctga aaacgccggc cacacaaaaa ctcgatgtcg
     5761 aaaaagccgt tggtgcgctc cgtcagaaag aacgcgcccc gggaaaaaca aaggagaatg
     5821 ccccggcatc cggccgaacg gcgatgacgg acgccgaagt caatgcgcag gccaatgaat
     5881 atatcgaaac cgtgtggtcg agggtaaagc agaactggtc cataccgcaa tccctgctgc
     5941 cggataacaa tatcacgacg attgtggatg tcaggattgc ccgcaacggc gccctgttgt
     6001 atgccggttt tgaaaaacgc tccggcaacc gttatttcga tgactccgcg ctcagagcgg
     6061 tcaaaaaatc cggtccgttt cctccgttgc cccattggat cagggataac catattgaaa
     6121 tcggcatccg gtttcattcc tcggaactgc gctgatctcg tgtgtgcagg agaacgtttt
     6181 ttaaaagaaa aaggtagggg tatcacgtga caaaaagagt tttgatttta acgttgatct
     6241 tttttttctg cgcctggccg atctgcagca accgtgcaga ttccaaagtg tatgtggaca
     6301 tcgattctcc cgctttcaga ctgattccca tcgccatctg cgattttcat cctgcgtcgg
     6361 gtccggtcgg ccatgtcgct tccgagacaa cggtttccga acgcgttaaa aaagatctcg
     6421 ccctgacagg actgttcaat attttgaaca aacaaagttt tctggaaagc cctgatgccg
     6481 tatcgacaac agccgtggga aaaattcgtt ttgccgactg gctggccgta ggcgccgact
     6541 atctggtcaa aggacgcatc tttcaaaagg atcgggaaat gaccgtcgag gcctatcttt
     6601 atgatgtcgc ccgcggtgag ttgattttcg gcaaaaaata ccaggcgccg gccgaaaaaa
     6661 ccaaggcggt cgcccgcgcg atagcatccg acatcatgct tgccctgatc aatgacgcgg
     6721 gagacttcaa caccgaaata gcctttgtat ccaagacagg cctccggtct gatatttatg
     6781 ccgtcagtta tgacggcgcc gatgtcagga aagtcaccca tcatcagtcc atcttaatgg
     6841 cgccgcgctg gtcgcctgac ggaaacggaa tggcctttac ctcattcaaa agaggacgtc
     6901 ctgaaattta tttcctcaat ttaaaaaaca ggtcggaaaa aagactggca tcttttggag
     6961 gattgaattt atgcggttcc ttttcccccg atggtcgaaa actattaatg accctgagca
     7021 aggacggcaa cgaagaaata tatgccctgg atgttcagac gttacagctt cagcgcctga
     7081 cccgccattc cgccatcgat gtttcgccgt catggtcgcc tgacggtaag cagattgttt
     7141 ttgtttccaa tagaggaggg tcgccgcaaa tttacactat ggatgccgat gggaacaacg
     7201 tgaaaagaat tacttacaaa ggcagttata attcctcgcc ctcctggtca ccgcgcggcg
     7261 accgtatcat ctacgaagga ttaaccgctg accgatatca aatcttttcc gttgacacgg
     7321 aaggcaacaa tccggcgcaa ctgacctctg acaatgccaa caatgagtcg ccgttctggt
     7381 cgccgtcggg gcgtcagatt gtgtatgtgt cccgcaaaca ttccggcagt caaatcctca
     7441 tcatgaatgc caacgggacc aatccgaggc tgctgtatga gcaaagcaac agactcgcca
     7501 tgccggcatg gtcgggacga ctccgatagg caaatttaaa tctcaaaggg gttgttattt
     7561 tcttttcttt gatgtataaa acacgcatat ttagaatgta tttcggtgaa acgggagtca
     7621 aaaacagaca aacagcgttt tgttgcatgg ttttaacgtg aacgattctt ggtcgtcatt
     7681 ttctgatcaa tataattttt gaacaccttt aaaaaaggag atgaagcaat gaacatgcaa
     7741 atccatttaa gacttgtttt agtcgtattt atagccggtt tgatgatgat cgcgggttgc
     7801 gccgaaaaaa aatccgtcat caccagcgat gccgcacagg aacagcaaac caccccggca
     7861 cagacgaccc cctcgcagga ggatatgtca tcgaaggata tcaccgggtc ccagattgca
     7921 tcggaggaaa agtcggtgga cctggaaaaa atggcgcggg cacaatcccc tgtcaacgat
     7981 atccattttg atttcgacag ttccagtatt cgcccggatg ctcgtgagac tctccgggaa
     8041 aatgccgact atttcatgaa caatagaatt tcgtccatca tcattgaagg gcattgtgat
     8101 gaaaaaggca ccaacgaata caatatggcg ctgggagaaa gacgcgctca ggaaacaaaa
     8161 aaatatctgg tgaatctggg ggtgaaagaa tccctgatgc aaaccgtcag ttttggcgaa
     8221 gaaaatcctc tggatccggc ctccaatgaa gacgcctggg ccaaaaacag acgggcacat
     8281 tttgcggtca acccctaacg ataaagcgag gtgaagcctt tggaaagatt tcgttatttc
     8341 attttatttc tggtggtcct ttttctggcc ggatgcgcct cctccggaga tttaagggcg
     8401 ctgcgttcgg agatgagcca gcgaacggat gaaaaaatat cctctctgga agcagaggtg
     8461 aacgcgttga aggaaaacgc cacggcactg gaatccatgc gcaaagggca ggccaatacc
     8521 atggcggatc ttgccgaatt aagggaaaat ctccagcaac tgcggggtca ggtggaaacg
     8581 ctcaaaaaag acgcgtcgca gtcgtccagg aaagacgaac agcttctgaa taacatgttg
     8641 ctgcgaatca atttcatcga aaattttctg gaaatcggca gcaaaaacac gtcggacgcc
     8701 tcggataaaa cgggcaaagt caatggcggc tcggtcgcaa aggataccgg aagcaagcag
     8761 gataaggata aagcgtactc agccgcctat cagatttttc aggaaggaaa ctatgcgaaa
     8821 gcgcgaaacg aatttcagac ttttttgact acctatccga acagcgaata ttcggacaac
     8881 gcacagttct ggattggtga atgttatttt tttgaaaaaa aatatgaatc ggccattctg
     8941 gaatatgaaa aagtcaccaa aaattatccg aagggaaaca aggtccccta tgctcttttg
     9001 aaacagggtc tttcctttct gaaactgggg gataaaacca gcgccaaatt actcctgcag
     9061 gaagtcataa aaaattatcc gaacaccagc caggcgcgta tcgcgcgctc caatttgcag
     9121 gacatcaaat agtcgttttt caccctctgc aagtatctat tctaaaatat tttgttccac
     9181 atcggatgga gcggtcagat taaaaaccga taaatctttc gtcttttcaa cccggacatc
     9241 ggagtaggtg actgtcagcg cggttgagcc gttcgccaat cggatcacaa gtttttcagg
     9301 caaagcgctt tgaccgccat aaaaatattg cacattatat ttttccgaac cggtttcatc
     9361 ggtgcaaagc agccgcatca gtttttgact gccttcctgt acccagatga tttgcgactc
     9421 actcgaggat ttcttgcatg tcatccgcca aagacggcct tcggatgaga catcctcggc
     9481 aagacgttct gtcgtgcaaa acggataggt ccccgtcaac accaggacca tatcttcgat
     9541 gtccatcgac cagggtagaa atttttccag attggaacgc gtcggtcgtc cccggtaaag
     9601 ctcttttttc gacggaacga aaacagtcat ggtatcgggt gtcgccgtca gcaacaagtc
     9661 gggattgccg atgaggggca gcacttccag gcgcacgtat cccggacgtc tcagaaccag
     9721 ggcggcccgg acatgatacg atgcgtcgac cgttttaaaa tctattttcg ccagggcttg
     9781 gaaaacatcc tcctcggcaa gcccgccgga tgtcgacaag atcctttttt ccggataaac
     9841 atcgcgcgca acacctgatg cctgctgtaa cgcacaggaa cagaggcaaa gcatcagaaa
     9901 catccatatc aagcggaaat atttttggat catgagttga ttcttatccg gaaagacctt
     9961 gtggtttgat tatcgtgtcc cgcggatatc ctcgcgcttc tttttcagga catcgtttcc
    10021 gggatcgatt ttcatccccc gctcatacat atcggccgcg tccttgagcc tgttctgccg
    10081 gacatacaaa tccccgaggt gttcaatgat ttcaatctca tcaggcagca gtgtccaggc
    10141 ttctttcaaa tatttttccg cgtttttcaa atcatttttt ttgaaatata cccatcccag
    10201 gctgtccagt aaataaccat tgccgggttt gattttgagc gcccgcaaga tcatggcctc
    10261 cgcttcatca agatgcatgt cccttaccgc ataactgtat ccgatgaaat tcagcgcttc
    10321 ggcatttttc ggatcaatgt ccaggatttt tttcatcgac ataatgctct cttcaaaacg
    10381 gttggtctta tcgtaaagca cgcccagggc ataatgcaaa tccgtgcttc gaggcagcac
    10441 tttcaaaccg tcctgcagaa tggcttccga cgaggcgtta tcaccggttt catcataaaa
    10501 ggacgataac aacagataaa acgggacctg atcgttcttt ttcttaatgg atttttttat
    10561 gaccgccacg gcctccgtga ctttcccctc ctctttcaag atcatcgccg cgcggagttg
    10621 cgcactgaca tacagctcgg acgatgatgc aatgcccgta aattcctcaa cggcccggac
    10681 gttatttttt tgctgatcgt acgttgtcgc cagcaaataa cgcactttgt cgtccaggac
    10741 atttttctgt aaaatcgaag aaaactcggc ggcggccaga tcaaattttt tcatgtcaaa
    10801 atacagcaga cccagcgcca ctttgatatc ccggttatcc ggatcgattt tcaaaacttc
    10861 ctgaaattcc ttttccgctt cttcatattt tttcttcttg attaaaagct cgccggtccg
    10921 cacgcgtaaa ttaagccgtg tcggatcaat cgccagataa ttgcggtaaa tttcaatggc
    10981 cttatccagt ttttcctgtt tctcgtacaa agccgccaga tcgaacaggg ccatttcaaa
    11041 atacggattc agggacatgg cttttttaaa catcttctcc gcttcgtcca gccgtttcat
    11101 cttctccaga actttcccgt aataatagac gcccatgatg ttatccggat cgattttcag
    11161 cattttttta aatgtctctt ccgctctgtc gaatcttttt tcttccgcat aaataatccc
    11221 cagatacaga tgcgcaacga ggttcttcgg atccagctcg atgacttttt tatgctctct
    11281 gactgctttc gaatactccc gcatattcag gtacaagccg cccataatca aatgcacgtc
    11341 tacattgtcc ggatcccggg ccagcgcctc ttcgccatgg gccacggcgc ttttgacgtc
    11401 gcccttctgg gcgtaaagag acatcagttc aatgacaaga taggcggagt cgggatccgc
    11461 cgcataggcc tgctccagct cgcggatggc ctcatccaga tttccatcat tgcgcagaaa
    11521 aacactcagc agataatgat aagaggcctg ctgttgaaat ttatctcccg acggagcatg
    11581 ctggacgttc ggcaacgtca cgcagccgga aagactgaca aacataatga caacgaaaca
    11641 aatgaaattt cttttcataa ggtcttcgaa tccgttttaa cggcgctttc gcgatgatgc
    11701 gccggcatta tgatttctcc ggggacttgt ttgttttttt aatgattcgg gatgccggaa
    11761 caaccgacac ccccttttcc cgcatgactt tggttgctgc ttgaagggcc cggaccgttt
    11821 caggatacgg atgcccgatg ataattttag gagcaggatc attgctctgg gcaacatgca
    11881 tcaattgact gtaaatagcg tgataatcac ggtgatgatc gataaaaatt tttcgttcag
    11941 cgatacggaa acccatgtcg tccgcaatgg cataggcccg ggtatccggc gatgtgcgcg
    12001 aatcgataaa aaaaagtttc ttgcgctgga gttccttaaa aatgacggac agtttttcgt
    12061 cgtccatcat gaacttggaa cccatatgat tattcacgcc gacggcatgg ggaaccgcat
    12121 caatgttctt ccgtaattgc aacacaagct cttcctgact catgtccgtg aacaaggcgc
    12181 cttctcccgg tcgctccctg ggataagaaa ccggttccat aggcaagtgc acaaggacct
    12241 ctctgttcgc ctgatagatc atttccgccg cttcgcggga atgtcgttga aaaggtaaaa
    12301 tggcaaacgt cagatcggca tcgattttca gcaacgcttt ggccacgttc aggtcataac
    12361 cgatgtcatc gataatgatg gccacttcac gtaaccgtgc cggaggagat gtccgtttct
    12421 taaccgaatc gattttgggc gcggcgggtt tgacggcaac ctctttctcc tttgcttttt
    12481 caggaaccgc ttcctgaaac cgcacgacat aaatgatgcc ggctactgac aataggatga
    12541 gtacgaccag agttgccgta aaaatttttt tcattaaatt catgaatgct caagtagtgc
    12601 agaacggctg ctatttcttt atggatttca tgatattcca gctcttaaga atattcacgg
    12661 ccgtctgcag ctgattgtct ttttccaaat ccaatgggtc ttttgtgtct ttgctctggc
    12721 ccccatcgac ctttgtacca tcgtccttat ccggtttaat atgacccttg agatcttttt
    12781 cccggatccg ttcttcctta tccgtaacgc tttccgcctc ttccgccccc ttgataaaag
    12841 gcacgataat gtcgggggta atgccttccg cctgaataga acggccattg ggtgtgtaat
    12901 aacgggccgt cgtcagtttg agggccgaac catcttccag gggaatcacc gtctgaacgg
    12961 acgctttgcc aaatgtttgt gttccgacaa ttaaagctct tccattatcc tgtagcgcgc
    13021 cggcaacgat ttctgcagca ctggccgttc cctcgttgac taaaacaacg atcggacaac
    13081 taatttcatt attatcgttt ttagcgactg attttgtttc cattgccttc actcttcccc
    13141 gggtggaaac aatggtcccc gatttcagaa aaatatccga tacatctatc gcctgagtca
    13201 gcaagccgcc cggatcgttt ctcaaatcaa gaatcaatcc cttcatgggc ttgtttttat
    13261 cgtccagttc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgggcgta
    13381 tcttcgatgg gcgagacaac cgtcagaatg tccttgataa gcataatctc tatgccgaca
    13441 ccgccgaact gtccctgtgt ttccacctcc agttccttgt ataaatccgg tgtcataaag
    13501 gaactgtgcg gatccagcga ccggatcatg ccgttgatgg ctccctgcat taaattagtc
    13561 gtatcaacat catcaacata gttttttctg accatgtcca gaacttcgct gaaagttttt
    13621 atgtccttat aaaaattttt atccttcgcg gcggcactat tgtcgctact cagtcccaag
    13681 aggatcaggg ctcccacacc gatgacggcc agcagataaa actttatcga tagcttcttc
    13741 atcatattcc tcacaaaatt gaatatatac cgcattactt atatcacttt cttcaatatt
    13801 tatcatgtat ttgttggaaa atatctgaaa gcgttgagtg ctctcgaaga cggaaacgtt
    13861 ttgatattac tcgatcgtcc agctggtgcc gtcagcagaa tccttcagaa caacattcat
    13921 cccggccagt tttttccgga tgtcatcggc ccgggcccaa tcctttgctt cgcgggcgga
    13981 ttgtctttgt ctgatcaact cctcaatctc atcggcgttc aactcgcgtc ggcgcaactc
    14041 ggtttcccgg tacaacacaa agaattgatc tgcatcgctg ttgatgacgc ccagcaccga
    14101 accgaaataa ccaaaaaccc ggatggctgc ggccaggacg gcggttttgt cggccaccgg
    14161 catgcttttt tctttcatca taaaattatt catctgccgg accatatcaa aaacatagcc
    14221 caatgcctgg gccgtgttga aatcatcatc catcgcttca ttaaacttat ctttcagcat
    14281 ctggaaggca ttgacatagt catcgacgcg cccggacggc tgcgttgcca ccggtttgga
    14341 tccggcggct aattgtccct gaagttcttt catctgctgg agcgttctgt aacatctgaa
    14401 cagagcggct ctggcttcgg acagcgaatc atccgaataa tcgaccggac tgcggtaatg
    14461 gctttgcagc atgaacaaac gcaacacttc cggatgataa tgtttcagga tttccttgac
    14521 cggaaatata ttgcccagcg atttggacat cttttccgaa ttgattttta caaaaccgtt
    14581 gtgcatccag taacgcgcca gcggcttgcc ggtcgccgcc gccgattgag cgatttcatt
    14641 ttcatgatgc ggaaaaatca agtcctcacc gccgccgtga atatcaaacg tctctcccag
    14701 ataacgccgg ctcatggccg aacattcaat atgccagccg gggcggcctt ttccccacgg
    14761 gctctcccaa aagggttcgc cttccttgct gtttttccag agtgcaaaat ccaggggatt
    14821 ttttttcttg tcgctgacat caacacgggc gccggccatc atttcttcca gattgcgacc
    14881 ggataaaccg ccgtactgtt tataatcact gatagaaaaa tacacgtctc cctcaacgca
    14941 atacgcataa cctttgtcct ctagggttct aatcagagca atcatttcct gcatgttttc
    15001 ggttgctctg ggagtaatgg tgggtgtccg gacacccagc gccgccatgt cttcatcgtg
    15061 tgctttgatg tatcgttcgg ctatatccgc aatactcacg ccttccctgt tggcccggtc
    15121 gataatttta tcgtcaatat ccgtgaagtt tttcacatag gtgacgttat agccctgggc
    15181 ccgcagatat ctgacgacca cgtcgaaaac aaccgccgca cgggcatgcc cgatgtgaca
    15241 gacgtcatag gcggtaatgc cgcacacata aatgccgacg gcgccctcct gaatgggctg
    15301 aaaaacttcc ttttttctgg tcgctgtatt gaatatcttc tgaggcattt ttttcacctt
    15361 tacgcatcag ggaaagagcg gcgcgatttg caggccggta tcttccttaa atccacacat
    15421 gatgttcata ttctgcacgg cctgtccggc cgcccctttc accagattgt ctatggccgc
    15481 catgataatg acccgttttg tccgcggatc aacagccaac ccaatgtcgc agtaatttga
    15541 gccgcatacg gacgaaatat tcgggtaaac gccttcggga cacatccgga caaacgtctc
    15601 cccggcataa aagctgctgt aaagagcatg cagatcgccc aaagcaatat cttttttcag
    15661 tttggcgtaa atcgtgctga gaatgccccg ttttaccggc agtaaatgcg gcgtaaagga
    15721 aacggcaaat tttttgccgg ccagcgcgtt gagttcctgc tcaatttccg gcagatggcg
    15781 atgctttccg actttataag ctttgaagcc gccgtcaact tcacaaaaca gcgaaccggc
    15841 aaacggttcc cgtcccgcgc cgctgacgcc ggaggcggaa tccgcgataa tcgtggaaac
    15901 atccaataat tgctttttca gcgccggagc cagccccaga ataatgccgg tcggataaca
    15961 gccgggatta gccacaaatt tcgttttctt tattttctgc ctgtaaagct ccggaatgcc
    16021 atacaccgca tttttaataa atttggcgct ggcgtgcttg ccgtaccaga cttcataaat
    16081 tttcaaatct cttaaacggt aatcagcgct caaatcaatc accgtctttc cggcgttgat
    16141 aaattccgga gccatgtcca tggacacacc gtgaggcaga gccagaaaca ccacatcgca
    16201 aagcctgcat atttcacccg gttttgcgtt ctgatatttt aacgcggtca aaccctgtaa
    16261 agccggaaac acggtcgata cttcctgacc ggcaaaccgc cttgacgtgg ctgccactaa
    16321 tttaacgtca ggatgtcctg acagaattct gatcagttcc tgacccgtat aaccgctggc
    16381 gccaaatatt gctgccttaa tcataaaacg ctcccaaaaa aaagggaggc tggaagcccc
    16441 cctgtcggaa tttaacgttt ggagaattgg aatctcttcc gggctccggg ctgcccgtat
    16501 ttcttgcgtt ctttaacccg ggcatctctg gttaaaaaac cggccttttt caggaccgat
    16561 cgcaactcgg catcgtactc cagaagcgcc ttggaaatgc cgtgcttcac ggctcccgcc
    16621 tgcccggcaa cgcctccgcc ggtgacgttg atatataaat caaactgatc cttcttcccc
    16681 gtgatatcga gaggctgctt gataagcatt ttcaggctgg gacgggggaa atattcctcg
    16741 tagcttcttt tattaaccac aatattgccg ttgcctgctt tcatataaac gcgggcgatg
    16801 gcactttttc tctttcctgt tgcataaaac ctttgttccg ccatgatttc tccaatatta
    16861 aatctagttt atagttgatt gtccgttaaa tcgataatgt tttcggacac tgtgcttcgt
    16921 gaatatgagc attaccggaa taaactttca attttttaag catcctgcgt ccgagattcg
    16981 ttttgggaag cattccctgc acggcgatac gaatcaaatc ttccggtttt tcggcaagca
    17041 ttttccctgc ggtcgtttct ctcaaccccc cgggatagcc ggaataagaa taataaattt
    17101 tatccgtcat tttcttgccg gtcagaacga ttttttcggc attgacgaca acaataaaat
    17161 caccggtatc aacatgcggt gtgtatgttg ccttatgctt gcctcttaaa cggtgcgcta
    17221 tttcactggc aaatcttccc agcactttac cggaagcatc tgcaatgtac cattttctgt
    17281 cggctgtctg cgccttagcc atgtacgtct tcataataaa atcaccactt taatgaaaaa
    17341 tttaaagatg caccttatgc aatttcaaat tgcttgtcaa gaaaaatatt tgaacagttt
    17401 gcaataccgg aaatacatcg gatttcttct tttttgacaa atttttgata cctttaaacc
    17461 cgaatttaaa gaggcacatt ttttagaaac aaattaaccc caaagcggaa cgtcgaacta
    17521 atgaaccttg aatatgacac attcatcccg ttaagatgtg cgcgttccat tatcaccaca
    17581 taagagggga tattaaaaat atcagagata tgccgattcg gattgtgtcg cgattggtcg
    17641 ttattatcat aaccaccatc atcgccagtt cattaatgtt cgattgtatt ggcttttacc
    17701 ctgagcataa acagaagctg tttccaccgg gcccggtgac accactatag cgatcaacga
    17761 agcaatcggc aaaggataaa cgcttggcat tgttctacgg gcaattttca agacgtgcaa
    17821 tcgctcaatg cgatttgatt ttcaaggcga gggagttttc gcctgttgcg ccaacgccgt
    17881 ctttacggcc tgacgcgtca ggaaaagcat ttcccggtat ttttccgaag cacccagagc
    17941 gttcgtcgag gtgcctgacg tatattcctt gagaagccgc accttcgttt tggcgaatgt
    18001 ttccagagtt tttcggtcaa tggaattaac gaatagctct ataatttttc ggcgcatggg
    18061 gcgtgttgaa ttggccagca acaggaatat cgtattattg gccaccctga ccacaagatc
    18121 ctgctgtttt tcctgaagct caataacctt ttctcggtca tcaagactgg caagttcttc
    18181 attgaaaagc cctgcgatag ccttcacgtc gcgcggggaa aatctgttag ccgccatttt
    18241 gagtatttcc ggaaagacag ccgtccagaa ttcccatatt tcatcaacga aataatcatc
    18301 aacaatcatt ttctgctcat tttcatcttt ctgagaaaag aggagactga gaaaatcgat
    18361 gccggcatgt tgcacatagt ctttaacata gatgccatct ccctgtttta tatcgaggac
    18421 attcatggat tccaactgtt ttaagccgat ccgcagagaa ctcaaatcga ctttcatctg
    18481 tctggatatt tcttttgccg aaggaagctt tgttccgggt ttgagcttat tattaaaaat
    18541 atccctcatg agaatgcgaa caactttctc cggaacttgt tggttggcca tatacccttc
    18601 ctcctttgtt tataatttta tacaattatc aatatcttgt caaacaataa ttcaattata
    18661 ataattgata tataaaattc ttgacattta atagtaattt gttatgctga ttttactgat
    18721 ggtggatacc ccctcgttaa ttcttttatg atttgtaaag aaaggcatga gtttttaagg
    18781 gaacaatgat tgtagcagga tgtgatgtcg gatctcttac cgccaaggca ttgatcatga
    18841 atgagaaggg ccggcttgcg ggagagatca cccgggtgag attgtctacc ctggattcgg
    18901 ccacggaagt aatggaaaaa gccttggcat cggcaaagct ccgattttcc gatctggact
    18961 actgctgcag cacaggctac ggccgttacg aaattccttt cgcccagatg aatatgagcg
    19021 agatatcctg tcacggattg ggtgccttcc gtgcggaccg cagcgtcagg accattattg
    19081 atatcggagg acaggactgc aagattatcc tgatcgatgc ggaaggcaat gtccgtgatt
    19141 tcatcatgaa cgacaaatgc gccgcgggca cgggaagatc attggaaatg ctggccaaaa
    19201 cgatcggcgt cgaccttgag acgttaggaa gcctggccgt taaatcgaag aaccccatca
    19261 gtattaccaa caaatgcagt atcttcatgg aactggaagt cctcaatcat ctctacgcaa
    19321 gaaaaaaact taaacacatc gcctgcggca ttgccgatgc cgttgccaaa cgggttgcag
    19381 ctctggccaa agccattgac gtcgagaaaa acatttgtat caccggcgga gtatccaaga
    19441 atcccggcgt ggtgagacag ttggcttatc atatgaacgt ttcatttaca tcgcttcctg
    19501 ttgatcccca aataatcggc gcaatgggcg ccgccgtcta tgccatggaa acgttgaaag
    19561 cgaaggtggg catgtgatta ctgcgggagt ggacgtggga actcgattta tcaaagtttg
    19621 cgtaaccgat ggcgagcatc tgcgcgggtc agcctgcgcg gagataggtc cccggttcga
    19681 tgcccttatc cgttctttgt taaaggaagc gcgggttcaa gcccgggcgg gaagatggga
    19741 aataaaaaaa attgtcgcca ccggtttcgg cggccatttg gtgaaaaagg ccgcctttac
    19801 attgggagaa gctgcctgcg ctgcccgatc agccttcttt tttgacaggg atattagaac
    19861 cgttattgat ctgggggggc tttttatcac ggtttctacc attgataaaa atggttttct
    19921 ggagaccact catgtgaacg agagatgcgc cgccggaagc ggcaagtttc tggaaatggt
    19981 cgctgaggcg gcagaggtac cttttactga aatttcttcc tgcgccgccg cttcccgcca
    20041 accctactcg tcctccagca gttgcgccgt cttcgccgag agcgagatca tttcgcaggt
    20101 caataccgga atgaaaccaa gcgatattct ggccggcgtt atccgggcca tagcctcaaa
    20161 gaccgcttcc ctgatcgatg gcgccggcgc aaaggacaaa atcgttttga tcggcggtct
    20221 ttccaaggtt tcggcagtta gagacatgtt aacgggactt accggcagaa gcatgacgac
    20281 gttgccaata gacagccagt tggttgccgc ttttggcgcc gcactcattg ctcagggcaa
    20341 gatcatccgg aaaaggcagg gtttatctgt cacaggagaa gccgcctgat ggaattgacg
    20401 gattgcacgc ctttgatgaa acaggtcaca gagatggtta gagatcaacg caggggtttt
    20461 tcgcgtcatc tttccgatca tggtgaagag gcgcgccgga tcggcttttt atccagagat
    20521 aaaaagcctg acagggccgt gattgttacc gccgaggaca ctgcgcttga attgggatca
    20581 ccgcgacatc cctctgtcag cacgtttctt tggacaaaac aaaaagatct gctgagcaga
    20641 gcgatttgga cggccggcag agatttcccc cagatcaaag aaagccccgt cagttttgcg
    20701 caggtgatca tggtggaatt agaaggtgac tttgatccca cgggtcctaa tatacaaacc
    20761 ctctcaaatc tgacgaatcg gattccggga tacatgaccc ggagcattcc cggcaagata
    20821 tggatcagga tccatcggga tctcctggcc aggaaatttt ccctccagtc cctcggacag
    20881 tgtcttatac aggcctatca tgagtctatc cccgggctga aaaacatcga agtcgccctt
    20941 atcgccgata atgccgactt cgtggggcat cttattccga ttcataaccg cgcccgcatc
    21001 atttatggcg aaaataaaaa gctggttctg gaagcggacg gaaccatcag ttgcgaggat
    21061 cttgactgcg aaaattgcac ggagaaaccc acctgcgata ccttgcggga tgttattgta
    21121 aaaaggagaa cgtgatcatg aacgaaccga ttatcctcgc tttcctcggg aagggaggcg
    21181 ttggcaagac ggtgttgtcc gctcttacgg gaacgatcct ttccgcttgc ggcaaaaagg
    21241 tcctttttgt ggatgccgat ccggccatgg gattgaccag cgccctggag gtttcggaca
    21301 tcaagaccat cggcgccgcg cgggaggaaa tcatccgtca ggcaagaatt tccagttctt
    21361 ctgaagaaaa agagaagctg gccgatatcg tcgactatct gctcatggaa gcgctttacg
    21421 aaggtcaggg tttcgggatg gtggcgatgg gacagaccaa caccctggga tgttattgcc
    21481 ccgttaacaa tcttctgcgc agtacgatca agtcgatagc atcacagtac gaagcggtga
    21541 tcatcgacgc ggaagcaggc atcgaacagg tgaatcgcca ggttgtggaa tcggttcatt
    21601 atcccgtcat tgttacggat aactctctca gaggagccag gacagcggtg atgatttcca
    21661 gtttactgaa aaaaattccc cagctgtcgc cggtaaaggt gggaactgtt tttaaccgtg
    21721 tcgatcaggc ggatgaaaat ctggtgaaaa tgattctgga ggcgggaatg gactattacg
    21781 gcagcttgag accggacaac gccatcagcg cactggacag gcagggcctt tctatcgcga
    21841 aaattgccgg cacatccccc gctgccgccg ggatgagatc cgttctggag caaattggag
    21901 cctttgggaa aatattaaac gttaaaaaat aagacaaaga aagaggacct atccctcaat
    21961 cctgttttaa atatattttt tggagtagat tatgcgagat gtttatgtcg ttgcggccca
    22021 cacaatcaag ttcggcaaat acctggagaa aacgatatcc gaattatcgg caatgacggt
    22081 ttccccctgt ctgaaagaag ccggattgga caagaaggat atccaggcgc tctggttttc
    22141 caattccgcc tggggtgaat acaaaaggca ggtctgcatc cgcggtcagg tggctcttcg
    22201 tcctttgggg atcgatgcca ttccgatcac caacctggaa aacgcctgtg ccggcggttc
    22261 cacggcgttt catcatgcct ggatgggcgt ggccagcggg atgtatgaca tcacgatggc
    22321 cctgggtgcg gaaaaaattt acgatcagaa ccaggcgctc gttttttccg gttttctgac
    22381 cggcctggat gtcggcaact ttggaaagac gctggaaaga ttgggcgacg tcgctttaag
    22441 cgaaaacgaa aagaaggcga tggcgaccca tatcgaaaag tacaagggcc tggcaaagca
    22501 aagctccggc aagggcagaa aaaaatcctt caaggacaag ttcatggccc agcgggatgc
    22561 cctggttgtc gctattcgga tcggtgagac catgggttac gacatgctca agaaactgcg
    22621 taaattaggc agcggcgacc attctccctt tatggatgtt tacggctatg aagcgcgtca
    22681 gcacatgaaa aagttcggca ccaccatcga acatctggcc gccatcgctt ccaaaaatca
    22741 ttttcattct tccctgaacc ccaatgcaca ataccaattt ttagtatcga cggagcaggt
    22801 tttggcggac cgcctggtca cctggccgct gacccgttcc atgtgtgccc ccatcggcga
    22861 cggcgccgct tcggcgattc tctgcgacga agcaacggta agacgcctgg gattgtcggg
    22921 ccaggcggta aaagtcaggg cgtcgattct gggttccggc atggccagag atcgcgacgg
    22981 tatcgacatc ggcgaacggc tgagtcgcct cgcctatgaa aaggccggcg tgggacctgc
    23041 cgagatagct tttgccgaag tgcacgatgc caccgcctac ggagaacttc atcaatgcga
    23101 gtcgctgggg ttctgccgtc tgggcgaagg aggacgatat gccgaaagcg gggcgacgaa
    23161 attgggcggc aagcaacccg tgaatcccag cggcggtctg gaatcgcgcg gccatcccat
    23221 cggggcatcc ggccttgcac agatccatga actggtgacc cagctcaggg gcgcggccgg
    23281 gccaagacag gtgtccgggg caaaacttgc aatcgctgaa aatggcggcg gagccctggg
    23341 agtggaagag gccgcgatgt gcatccatat tctggaagcc ccgtcattga aataatctgt
    23401 atgtaaggag caaagcagtg aacagaaaac taagaaagct ggatctggga aaaaacattt
    23461 tgaaatcgat ctgtttgttt tccatcaatg aaatcaggag aggttccttt ctgaaaaaca
    23521 ttcgcggcgt ttccaggacg gaaattcgac aggaaatgtc gtggggagaa ttactggaag
    23581 aaaaggcggc ccgttacaag gataaaattt ttcttatctt tgaagacaaa acgttcacct
    23641 atcgtcagat gaacgcaaat gccaaccgga tagccaatta ctttttatct tcaggcgcag
    23701 ggcagggcaa gggcgtggcg attcttatgg acaattcacc gagcttcatc gatattttca
    23761 tcgggattca gaagatcggc atgtacgccg tccccgtaaa cacatcgttg aagggagaca
    23821 gtctcctgta tatccttaac cattgtgacg ccgaatatct ggtcatcgat gaaacgcatc
    23881 tggagacctt gaacaaaata gccgaccggt tggataagat aaaaaccatc atcattaatc
    23941 catccccttc ttcatcgaga caatatccgg cgggatatca tgtccttgat gaagcctata
    24001 aaggatcgcc cgcgaatccg gggcttggct accgcaaaga cgatatctgt tttattacct
    24061 atacctccgg gacgaccggg cttcccaagg gcgttgttta ccgctaccgt aaatcatcgg
    24121 tgaaactgtt gtcgctggtc gccgttatgc ttttcaaaaa aagcgacgtt ctctatacgg
    24181 gcatgcctct tttccacggc aatgcccttt tcgtaacggt gacctgcgcc ctgcatgcgg
    24241 gagcccgggt tgttctggca aggaaattct cggccagccg tttctgggat gatatccgaa
    24301 agtacaacgt tacgaccttt aataccatcg gagccatgat ccctattctg atgaagcagc
    24361 cggaaaaacc ttcagaccgc cagcacaatg ttcgttttat tatttcggcg gcctgtcccg
    24421 ctgatctgtg ggaaaaattt gagaaacggt tcggactgac catctatgag acctatggcg
    24481 ccgttgacgg aggcggcaag atcatcctca acctgggcac tgcgccggtg ggatcgatcg
    24541 gcaagccgcc catgaaagtc aagtaccgtc ttgtggatgc caacatgaaa gatgtgcccg
    24601 atggtacgcc gggcgagctg gtctttcccg tttccaataa gaaaagcaca tcctccgtcg
    24661 agtactataa aaacgaaaag gcttcggcgg aaaaaatgaa cggtgactat atcttcaccg
    24721 gggatttgat cagacgcgat acagagggct tcctgtactt tatcggcaga aacacagagt
    24781 cgatgcgcat taaaggtgaa aatgtatcgg ctttcgaggt ggagcaggcg atccagaaac
    24841 attccaatgt tctggaggcg gctgtttacg ccgttccgtc agaactggcg gaagatgata
    24901 tcatggcctc catcactctg gtggaaggca tgacgctgaa agaaaccgag ttgatcgagt
    24961 atctgaagga aaacctgccc aagttcgccg tgccccgata tgtgaagatc gttccggaat
    25021 tgccgaaaac tgaaacgcac cgggtcaaga aaaaggaatt agaggcgctg aaggtcgtca
    25081 ccggcacctg ggacgaaaac aaaaaagcct atgtgtgacg gtttctcgat gcgtctgatt
    25141 ataaaaatta ccattatcta gtaacaaagg aggtcaaaaa ctatgagcaa gatcaatacg
    25201 ggtttctggc agaagatgtt ttttgtcggt tcgctctgga atctgggtat cggcataacc
    25261 agtctgttgt ttacggattt tatgctcatg atgatgttcg gaaaggggcc gatagaagac
    25321 aatctcctgg cctttatcaa tggtacggtg ccggtaacgg acaacctcca gaccttgata
    25381 tttttcaggt tctttatgat cgctgtcatc ctcttcggca ttggttacta ctgggtctcg
    25441 cgcgatctcc tggccaaccg ggccgtgatc tggctggggc ttgtcgccaa gctcatcatc
    25501 tttttcacct tcgtctacta ctacgccctg gagcaagcgg cctggtttcc cgtcttcgtc
    25561 ttatcgggcg atttcgtatt ctcgatcttt tttgtagcct tcctctggaa gaccaaggac
    25621 ggcatttatt gaccctttaa ccccacaagg agagaagagg tataccatgg caaaacaaac
    25681 aaccgacaat ctttccccgc tctacaccta ccaggagggc atggaatgga atcccgtgga
    25741 aaaggtgatc atggaacggc gaagcatcag gaacttcaag aaggaaccgg tgcccgacaa
    25801 cctgatccga cgggtgctgg aggcggcaag attcgccccc acggcgggaa acgcccaacc
    25861 ctggaagttt atcgtcgtca aggaccaggc gctgattgca gaaatggagc gggcaacgat
    25921 acaacttagt aaattactca tgtggttcgt ggattataat agaaacgcct tccggcgcat
    25981 atttctggcg ccctatacga agtttatgac ccggctgatg cccaacaaac tccatcccat
    26041 ccccttcaac ctgctccagc agatcgccgc cgaaaagaca gccgtgtact ataacgcccc
    26101 cgttatgatt ctccttttga tcgataagcg ggggatcggc aatccggtgc tcgatgccgg
    26161 catctgcggt caaaacatcg ttctggccgc tcacagcctg gggttgggaa catgctggat
    26221 cggcatgatg acggttttga tgatgaatcc cctgtggcgg agaaaattgg gagttaaatc
    26281 cccctatgag ctgaccgact gtattgtttt gggctggccg aaaggcgact atgacggcga
    26341 ggtgaccagg gaagtgcagc tcgtcgaatg gcttgaggcc ggcggggaga aacgaatcga
    26401 aaggcagggg gtgtgatatg ttagatctgt cattaggaaa gaagggaaag attcccaatt
    26461 actttaaaga gactcagatg acctacgggg ttgtcgtctt cgatcctacg ctatgtaccg
    26521 gctgcggcat ctgcgtttcc atctgtcccg cccggggcct gatgcttacc aaaaggcagg
    26581 acagtgccaa aaagaagata ccgctggtga tcgaaaccgc accggggata tcgctttgta
    26641 tggcctgcgg cgactgcaca gcggcctgtc cggaggcggc catccgcatc aaacgcggat
    26701 tcaacgccgg gttcttcttc cgcaagttat cacagcagcc ggagttaact ttgccgaaaa
    26761 aatattaata aaatcagaag gagagtttca ttgaaaaata accccaagcc gtttcaatat
    26821 cttaaatgga gctccagttt tctgaatttc ggatattacc ttacaacttt tccgaatttg
    26881 agttactggg ctctcagact tacccggcct ctctatgtca aacgcgaaac attcttcaat
    26941 cacaaattca gatatgcttt cctgattctg ggaggaggaa ttcttccgcc cgaggaaatc
    27001 aaagcctacc actatggggc aaaagccttt gttgataata tgaaaaatgc tctaaaagct
    27061 aacgaaaacg gtaagcccgt cgtctgggtg gaatggattc tttcctccga aatcgtaaat
    27121 gcttttgacg cgtttccgtt caatccggaa acgcttaatg tgttcggaaa cttgtccgga
    27181 caggacgttc cgtcgctgct catagaggaa gcggaaagcg cgggttttgc ctcagaatac
    27241 tgctcggcca ttaagctgac ggcgggagcc tacatgttgg ggcaaactcc caaacccgcg
    27301 gcaatcattg ccgggtcgca tccctgcgac accaacgttg ccgcctatca gggaatacgc
    27361 catttgacgg gtgccccggt gttctttttc gactcccctt attggcgcga tgaagacgcc
    27421 ttcgattatt atgaaaaaaa tatgtggcgc ctcatagagt tcctggaaaa aactcttcac
    27481 cagaaaatca actgggagag gctcaaacag attgtagatc atgaaaataa attcaacttt
    27541 tatatgcagg aaatctgcga aatgatgcgg gcaatcccct ctcccgcaac gatgatcacg
    27601 ccggtttttg cctgggtcgt gcgggaactc gccatcggcg atcctaacgg caccgaaatg
    27661 gccagacgca tgtacgaggt cgtaaaggat cgctttaaaa aaggtgtcgg tgtcgttaaa
    27721 aaggaaaata tccgcgtttt gtggtggaac ccgccgattg ctttttttgc ctacgtcttc
    27781 aagtggatgg aagatgaatt cggcgccgtc gtcgtcaagg attttatcgg cgatgtccag
    27841 atgccgcaga tcgatacgtc ctcggaaaaa acaatgattc gtgacatggc taaaaatcat
    27901 ctttacctgg cgatggccag acaatgtcac ggaccggtgg aatttattac gcaagagctt
    27961 gagaaggcca tcgacgagta ctcgcccgat tgcctgatct tcgcgggaca cgccggctgt
    28021 aaacacggct gggcagtcat tggtattatc aaagacctgc tgaaaaaaag gggcttgccg
    28081 tcgctgttca tgaatatgga tatcatggat cagagacatg caacggagga tgacatcaaa
    28141 cgctggataa cggaattttt caggagcaac ggattcgtat gaaaacgatc atcgacgaaa
    28201 caagagatct ggcattatcg acacataacc attatctcga agaagccaga aaacagggca
    28261 aaaaaatcat cggatatttc tgttcctata tgcctgagga aattatccac gcggccggct
    28321 ttgttcccta ccgcatgcgg gccgtggaaa gcaaagggac aacccgtgcg gatgcttttt
    28381 attcttcgat caactgcact ttcgtcaagc actgtttcga aaaggcgctc aaaggagatt
    28441 ttgcgttttt ggacggcgtt gtgttcgtaa acggctgtga tcattcgcgg cgcatgtatg
    28501 acaactggcg tttcgcaaag atcgaccctc cttttcttca catgtttttc gcaccgcata
    28561 tgatcaatga agcagctctt gactattttt ccctggagtg cagcaagcta aaatcatcca
    28621 tcgagaaaca ttttcatgtt tccataaacg acgaatcgct caggcgctcc atcaaccttt
    28681 ataatcagaa gcggcgtctt ctggcccaga tatatgccct gagaaaaatg aaggatgtgc
    28741 ccataaaagc cagtgagtta ttgggcgtta tgctggccgt cacagcggtt cccgtagaac
    28801 gggccataga catcctgtcg aatgtgaaaa attatatcac cggtcgcgtt gtgtccaaac
    28861 cccaggattt acggctgttt ttctccggag gctgcattga ggaaatggat catctggaac
    28921 tcatagaaaa ctgcggggcc gtcatcgtgg ccgataacat ctgtctggga tcgcgtcatt
    28981 tccttgatga ggtcaaaaat gacggcattc ctttgcagga cattgccgac agatacttaa
    29041 gccatttatc ctgtccccgg atgatgggtg attttgaacg gaggtttaat tatttgttgg
    29101 acgtcaggga tgaatacggc attgacggca ttattatcga aaaattaaag ttttgcgata
    29161 tttggggcgg ggaaatatat cttcaccgca cagaatcaaa aaaaagaggc ttggcgctct
    29221 tggccttgga gagggaactc tacggcgggg gcatcggaca aataaaaaca agggttcagg
    29281 cttttctgga gaagttgaga aacaaacagg caaccgatga aaatctggtt caggcggcag
    29341 gggctgatta taaacctaaa atacaataaa ccgggagggt aaaacagatg aaaaaaaagg
    29401 ggagcggtta tgaatatata atcgtcggta cgggaccggg gggcgctccg gcagccaggg
    29461 aactggccaa agccggtaag aaagtgctca tggtggaaaa aggcgattat cacaaaaagt
    29521 ttctggggct tcctttcggc atcaggctgc ttgagcgata catgctcttc gcccgctcca
    29581 gggaaggcgt cattatcgaa cgcggcatta cggtcggcgg ttcttccatg gtttatcagt
    29641 ccaacgtcat ggatcctccc gtaaaactga ttaataatat gggggtcgat ttcagaccgg
    29701 aagccaagca gcttaaaaaa gaaatcggca tcgaggttct gccggaaaaa ttttaccgca
    29761 acaacaaggg actctaccgg atgctggcga cagccgataa aatgggcatt cccttccggg
    29821 agcaggagaa gttcgtccat gccgataagt gcaaactggg ttgtgactgg tgcatgctgg
    29881 gttgtccgga aaacgcgcgc tggacaacca gggaacacgt ggaagaggct aaatcatacg
    29941 gcgccgagct catcttcaat gctccggtca acaaaatcct cttcaatgcc ggaaaaacaa
    30001 aggcaaccgg cattcaactg aagaacggca ccgtcattca tggcgacaat atcatccttt
    30061 ccgccggcgg tatcggcagt cccgctgtat tgttaagatc gggggtcaaa cgattaggga
    30121 cgcaggatgt gggaacaaga ttctttatgg atcccatgaa catcatgctg ggttacagca
    30181 aggatcccga cggcggcttg tggggaatac agacgttcag ccacgccatt gaatccatgg
    30241 ctcagagcga aggatttatg atcggcaact gttccgcttt tggaacatgg acggttttgt
    30301 cttttttccg catgcagaca gcccgagaaa atctgccgaa atttcccatc atgaaaagag
    30361 gcatggggtt atttctcaaa ttgtccgatg aagcccacgg acaaattttc cccaatgaga
    30421 aaaccagcaa gcccttcacc gcaaatgacg aaaagagatt gaaaaaagga acggacatta
    30481 tgcgggaaat catcattaag tccggcggcg atccgaagac catttccatc ctgaaatggg
    30541 cgggaggaca tcccggagga acccttgcca tgggacaggc cgtgaaccgt gacttttcga
    30601 cggagtttga aaacctttat gtctgcgacg gcagcaccat gcccgtatcg ccgggggctc
    30661 cgccctcgct gtccatttgc tccatgtcgt tgctgttggg caagatgctg ttgggcaagg
    30721 tgaaggctca agaccggctt atcaaggcag cgggctaaag aacggactca taacgaaaat
    30781 aatgagatgc tctgatagat actgtcacaa tgggattgct ggaaacacgg ataggcgata
    30841 gtcgctgaaa agaatatctc atgatggaga aactaaacaa ttaacaggca cagggtggct
    30901 tttcgccacc ctgtaatcgg cacattacat gtttttcgcg gcctgttcga tgccttttcg
    30961 aatcccgtcg agtttttcct ggctcatctt cacggaaata cccataacca gcgtgcgccc
    31021 cagaatatct tcggcctgcg gaatattttc cttcctgtac tccacctttc ctttataagc
    31081 cggattggtt aacggatatt ttttcgagtt ggccgtcgat ttcgccagga aatgttccca
    31141 gttcggcaca tagtgccata gatttctctt gtaacagacg gtatccacgc cttcggcagc
    31201 cagcagattc tggaatttct gtgcctgatc ttcactggga agattaaacg ccaggaacgt
    31261 ggcggaatcg ccttcgggat ccggtttgac gcggaatttc acacccgaaa cattggccag
    31321 aacatccatg atgaatttct tatttttctt ctgttcgccg atgagataat ccagcttgcg
    31381 cagctgagcc aagcccaaag cgccctgcag ctcattcatc cggaagttga aaccgagaat
    31441 ggttcgtcct tccagagcac ggctgacttt gggattatgg tcatggccgt ggtcatgata
    31501 ccattccgac cgcagataaa attccttgtt attggtaatc accatgccgc cttcgccggt
    31561 ggtaactgtt ttgacgtaat caaagctgaa cgttcccata tcgccgaagc tgcccagctt
    31621 tttgccttta tagctcgcgc cgcaaccctg cgcgttgtct tccagaacca gcagcttgtt
    31681 tttgcgggcg acgtctacga tcttgtcaat gtttgccgcc gaaccgcaca tgtgcacggg
    31741 gatgaccgcc ttggtgtaag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31861 nacatccata acttctttga tttcttcttt tcctattaat tctgcacctg ccatatcgtt
    31921 aaccttcctt tctaaaatgc ctgcgtgtaa attgcgatca tatcgtcctt ggtcagttta
    31981 cggggattat tctccagcgg tctggctacc gttactgcca cgtcggccag agccggaaaa
    32041 tccttttctt tcacaccgaa tgttgaaatg gtgtcgtgga tgccgcaatc ttcaattaat
    32101 gcttccacag cctccagcgc cagataggcg gcctcgcgct caggcaatcc ttcaacattt
    32161 tcccccatgg cctccgcaat atccgtaaac ttttccagag ccgagggcat gttaaacgcc
    32221 atgaccgccg gcagcagcat ggtattggcc agtccgtggc cgacaccgta ttttcctccc
    32281 agcggatagg aaagcgaatg caccgccgta acgcccgcat tggccaaacc gataccggca
    32341 tataaactgc ccaaaagcat tctttcgcgc gcgacgatat ttttgccgtc gtgcacacac
    32401 gtgcgaagat tttcggcaat caacgcgatc gcttccaggg aaaacatctc gctcaccggc
    32461 gaagaattaa tggaggtata ggattcaata gcgtggcaca gggcatccag gccggtatgc
    32521 gccgtcggaa caggcggaag actgagcgtc agttccggat ccaaaagtgc cagctccgga
    32581 tacagatagg ggctgttcat gccttccttc tttttcaaat tcttgcgaag aaacaccgcg
    32641 gtaaaggtca cttcggatcc ggttccggca gttgtcggaa tcatgatttt gggaagtccc
    32701 ggtttgggaa ctttattgag accgagataa tcggcggctg cgcccttgtt tgtcgcgatg
    32761 acggcaatgg ctttggcgac atccatcgca ctgccgccgc cgatgccgat gacgatatcg
    32821 catttgtttt tcatggcggc ttttgcaccc tcatccgcca gttcaatggg cggctccgcc
    32881 tccacctttt catagactgt aaatttaatg ccggacggaa tcagaacgtt ggcgactttt
    32941 tccagcaaat ccgttttggc caggttctta tcaatcacca ccagcgggtt ccgggcatga
    33001 tgctccttga catggctcgc cagggttaat aatgatccgc tgccgaaaac aatttttttg
    33061 gcgccggtaa atgaaaatgt ttttctcata atgtcaaacc tccatcggta tccattgtat
    33121 tgtgacgttg tttttcatca gatatgcatc tttgatacat gcaatcaaac gattagaaaa
    33181 gcaatgttta tggccgcatt cactatggga actcccctgc aaagtcaagt ttttttgtgg
    33241 tgagcatacc actgcagttg tcggcagata ccgcggacaa tcttgacctc ccgcgacaaa
    33301 attccggcac gggtaaagaa gcggcgcaaa tgcatcatcc agtagtccgg gttttccgga
    33361 ttcaaaaatc cgatatcggc cagcaatgtt ttgatctgac cgtacatgcc ttccagctcc
    33421 gaggaaagcg ccagcttcgg cgttgcttca gacgccgtca ccgcaataca ggacgcagta
    33481 aatatttcat aacagagaat catcaccgcg tgggaaagat tgagagaggt aaattcccgc
    33541 gatgtcggaa tcgtgaccac catgtgacag agacgcaatt gctcattggc caggccggta
    33601 tcttccggac cgaaaagcag ggctaccttg ttcttctgcg aaatatccgc tatcgtctgt
    33661 gcagcctggc gcggcgtcac aaacggaccg cgggctttgc ccgtacgtgc ggtcgtcccc
    33721 acaatataat tgaacggact caccgcttcg gcggcatttt caatgtattg aatatcatct
    33781 aaaacatcgg cggccagatg cgtggaccgc tgtcgcattt cttccctgtc aaaatcatcg
    33841 gtccccacta caatcatctt acgaatgccc atgttttttg cggcacgggc tactgcgccg
    33901 atattccccg catatttcgg tttgtataaa acaacactga tattttttaa ttgcgttttc
    33961 aacatggttt tgtttcctgt ttcgaaagta ttctaaaaaa tcgttattcc gttttgtctg
    34021 tataaatcag ccctcaacga tttgcacgga gaattttcgc ctgtcccggc cgacacccgg
    34081 acgggtgtat agatattgac aaacgatttc atttatccca tgatgcgact cgactctctg
    34141 gaatcgttca cctccatcat ccgccctagg acggtgtggt tatgagctta aatttttgtt
    34201 ttacgcttgc ctgaaaagat tggaacgctg acccgtatgg taaaagtgaa aatatcatta
    34261 atctctgttg gtctggtctt gctgtttgct tttttcttat tgctgccgct gcaaagtttt
    34321 gcctatgatt gttccaccag agagggcaaa caatatctgc ggttgctgga acgtaaattc
    34381 gccgattacc ggtggacgga tcttcatccg cgggacattt cctgggaata tcacagaaca
    34441 acccgaaaca aacatccgct catgtttgtc cacttcggca atagttccaa aaactgcgtt
    34501 ttgtttttcg gcggcgtgca cggagacgaa ttaccaacgg cttacctgat gtttaaactc
    34561 gcccattatg taaaagagca tcccgccctg tttaaggatc aatgtattgt gattgcaccg
    34621 ctattgaatc ccgacggatt cttatccgct caaccaaccc gtaccaacgc cagcggcatt
    34681 gacattaacc ggaatttccc aaccagagac tggaaagcca aagccatccg ccaatggata
    34741 gccaagggaa aacaggttcg ctactacccc ggaaccagac cggcttcgga acaggaaacg
    34801 atttttcaga tggctttaat caaacgattc aaaccgcaga aaatactgtc tgcgcattcc
    34861 cctcttaatt ttttcgatta cgacggtccc tcatcggact tggattcctt tgaaaaatgg
    34921 atggagcaga tctgcaaaga aagtaatcac ccgttgaaaa aattcggtta ttatcctggg
    34981 tcgctcggca attacgcagg acatgaacgt aatattttta cgcttacgct ggaactgccc
    35041 tcctccgatc cgggacaaag cagcgactac ttcgaaaaat ttcaaccgtc tattgtaaaa
    35101 tttatccagc tgcccattgt cggatcgccg cctaatctta aggtgctccg ttaatatccg
    35161 cccgccttgc ctgacccgtt ctgcagcgtt tccacttggc tgtccgccgg ggcttctgaa
    35221 acgcgcccgc tcacacaagc ctggcttgca gtcctcaatc ggttctgacg ggatcaaata
    35281 attttttgag cagggactgc agggcataac gggtcagtgt attcatgtaa tgaggcgccc
    35341 gggccatcca tgcgccgatc agaaagacaa cacaggagac ggcataagcc agcggcccgc
    35401 cgatcacaaa aatcagcggt tcgtgaaacg aaacggcaag aatacttaag acaaatatga
    35461 tgggccagcc gattacgtaa ctcaacgcca tgaggactaa accgccgatc aaacggggtg
    35521 tcggacgttg cttaaactcg cttaaatcga cggggtcatc caggattttc cggatgaatt
    35581 ttttttgggc aagatattgc cccaggttct ttttgatcat aacacatcat ctccggtcac
    35641 aggcgatccg atgatcccgc acggtgtgaa ccagacatac atgagacatt ttttttctgc
    35701 aacggctttt ctgagacgaa cccctccttg acatccctct gccggataag ataaaaatcg
    35761 taaagaaaat taacatgaca cgatgataaa atgaactctt caaccaacgg ctgggatttt
    35821 ttcgatcgct tctattgtat ctcgcttcgc aaacgggaag atcgccgcca gtcggcccgg
    35881 aaggaatttg aaaaagtcgg cctggccaat cgcgttgaat tcgtcattgg agaacgtctt
    35941 tccgatgatt tcgaacgcga agtatacgag gcgcacatgc tgtgtctacg caagggcctt
    36001 gaggcgggag caaaaaatat tgtcgttttt gaagacgatg tcgaatttga tcgctttgac
    36061 ccggaacgcc tgcaaaaatg cactgatttt ttacgacgac atcccggttg gaaagttctg
    36121 ctgttgggag cattaatccg ttccagccgc aacacagacg acccttgtgt tcaaaaagtc
    36181 cgctaccaga gtctgaccca tgcctacgct ctaaacgctc cctatgcaaa aaccctggcc
    36241 tacgacccgt ggagcggcat tgtcaacgat acgcttttcc ggccgttgac ggaagatgtc
    36301 tacgctgtct gtcccatgtg tgcttttcaa aaaaatttta cctctgacaa ttacaaatat
    36361 ccccgactcg ccctgatccg tcgtttattc ggcgggctga aacgtattca aaaagcaaat
    36421 gaattttatc agcggtataa agtcggcgta atcgccgcac atcttgccgt tgtgatattg
    36481 attcttttcc tcctgtggcg gggctgattt tccatcatga ccataccgtc tgcggcaacg
    36541 cgtcaagccc gtggccgtaa aggatcatga tgttgcatct tgacgaaaaa gcacct
//
LOCUS       DAOSME010000022        36310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1549_length_36310_cov_24.695408, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000022 DAOSME010000000
VERSION     DAOSME010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 36310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36310
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1549_length_36310_cov_24.695408"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLG98_04980"
     CDS             <1..670
                     /locus_tag="PLG98_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32639.1"
                     /translation="TVKINDDYELGQQMYIWEMATAVAAHFMGVNPFDQPDVEATKKH
                     TRAVIADLDKRGELADEKPAITFEQAEIFGNINGATPADVIRNFMKQAKAGDYICLQV
                     FLIPSQETNEAVNSLRKVLACQYTLPVACGYGPRYLHSTGQLHKGDAGNGLFIQLTQD
                     FSLDVDVPDSIGEEKSFLTFSALKAAQAKGDRQALTEAGRRIIRIHFKKNPVDGLKTI
                     VNGL"
     gene            complement(806..1375)
                     /locus_tag="PLG98_04985"
     CDS             complement(806..1375)
                     /locus_tag="PLG98_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE32640.1"
                     /translation="MMTFTDLLKNRRSIRDFQDKNVPMNLIEEILQESTLAPSASNNQ
                     PCRFSIVHCRKTIKALSDESKANLLEDYAGNKKSLLPQYIDMLKDENFNVFYNAPCVI
                     FIIGSKSVKSLDVDCALAASYIMFSAVERGLGTCWVNLGSNIRDPQSRALLAIPDGCR
                     IVAPIILGYPKTIPPVSERHAPEILRVIS"
     gene            complement(1412..2404)
                     /locus_tag="PLG98_04990"
     CDS             complement(1412..2404)
                     /locus_tag="PLG98_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOE32641.1"
                     /translation="MNTKNMLRYISALTVIAAAVYFFYLQFKKNADAIGAWKFSINPL
                     EMSLAVIFGSCALLVGPLVWRLFVNSYLKKKLNYTESFVLYCTSAMFKYIPGKIWTYA
                     AQIALLSSKGISKVALLYINLVSFICLLFVAVPFFLYYYFIYVQEMSLGATLLILTLF
                     MLMDVVFIIANHTIVNYLIRPINRLFKLNIKPIRTDKIIFVYVQMLYSLACVLLGIAL
                     YFLARGVNMPLPFSQIIAMMATICVSLILGLLAFFTVGGLGVREGAMFFMLKQFSNIE
                     AALILPVISRFLITLVELLMAVTAIIIGLKYGYFHDIEKVKPAADSALPRESAG"
     gene            2618..3253
                     /locus_tag="PLG98_04995"
     CDS             2618..3253
                     /locus_tag="PLG98_04995"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOE32642.1"
                     /translation="MVLASGKTNSKTRTAIIEAAGKIFAEEGYLKATVRDICSSAGAN
                     LAAVNYYFGDKKGLYLSVLKYYQEMAFQAYPPDLGIRASQKPEEKLKIYIRMFLKRIM
                     DDGRPVWFGRLLAREFSEPTCALDVLVNETIRPSFQLLTEIVADVLGKTPREKMVRLC
                     SMSIVGQCLYFRHSQPIMTRIYPEATFGAEQIDEITEHIASFSIGGLRKAQ"
     gene            3271..4548
                     /locus_tag="PLG98_05000"
     CDS             3271..4548
                     /locus_tag="PLG98_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE32643.1"
                     /translation="MQKKIMLLFLIVLIPFAAAASEPAGGTASYDLDQCIAVALDKHP
                     SLKASAGDIRARESRVGQARSKYYPWLDVSAGYQKIGPTTPPGDSIDSYDQYSTGFNL
                     GVTLFDFGKTSANVAIQDLNVNASRADYDDVKAQIILNVKKAYYTLLQNQRNRDVAVD
                     TMQQFQQHLDQANAFFRVGTKPKFDVTKAEVDLGNARLNVLKAENAVRAARITLKDVM
                     GIPENADFTIVDNLSFQKQAWILDDILNKAYAVRPDLLSIIARREAAERSIDLARKDY
                     LPTLSGSAGYGFSGRDFPLGRGWNAGASLSFPLFSGFSTNYQIDEAEANLAILTANEE
                     TVRQAIHLDVQQAYLNVQDTAEQIAMAEMTVVQAQENYDLASGRYRSGVGSPIEVADA
                     TIMLNNARAGLNTALCNYRIAQAVLEKSIGAKP"
     gene            4545..5729
                     /locus_tag="PLG98_05005"
     CDS             4545..5729
                     /locus_tag="PLG98_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE32644.1"
                     /translation="MKKKIIVISVLLIAAFAAGILFYVNRDGKKAVYKTEKISRGEVK
                     SVVTATGTVNAVTTVSVGTQVSGTIRTLFADFNSPVKKGQLLAQIDPATFRAQVDQAR
                     ANLLSAQANLEKAGVAVVDARRTYERNKELFSQNFIARSDLDTAETALHAAQAQQKVS
                     EAQVAQARASLRMAETNLQYTNIISPVNGTVISRSVDVGQTVAASFQTPTLFSIAQDL
                     TQMQINASVDEADIGQIRAGQDVSFTVDAYPDTDFSGKVSEVRNAPTTVSNVVTYDVI
                     IKVDNPELKLKPGMTANVSITIETRPDVLRVPNAALRFKPAEVGEKGVSAKELQDIKG
                     TKVWVLENNQPKPVGVTLGLSDGNFSEIVKGDLRTGQEIVTDSLNKKGSSTTQRPPPR
                     FM"
     gene            5729..6421
                     /locus_tag="PLG98_05010"
     CDS             5729..6421
                     /locus_tag="PLG98_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32645.1"
                     /translation="MTLIETRQLVKTYDVGDEGVHALENVSVKIDKSEFVAIMGPSGS
                     GKSTFMNIIGCLDQPTAGDYFLDGQTISGLSRDDLAAIRNQKIGFVFQGFNLLSRTSA
                     LENVELPLLYNHVPARQRKEESLKALKMLGLEERAYHHPNQLSGGQQQRVAIARALVN
                     QAPILMADEPTGNLDTKTSIEIMELLVQLNRDSGTTIILVTHEPDIADFSKRIIRFVD
                     GHVVSDEEVQKS"
     gene            6418..7647
                     /locus_tag="PLG98_05015"
     CDS             6418..7647
                     /locus_tag="PLG98_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE32646.1"
                     /translation="MISIFSTLRIALRALWVNKMRSALTMLGIIIGVSAVIIMLAVGT
                     GVSRKISSQISSIGSNLIIIMPGSTTQGGVRMGGGSQSTLTTGDAEAILRECSAVAAV
                     APMQFGTAQVVFGNQNWSTGIQGTTPGILEVKDCGLSAGRNMSDQDIRSATKVCLLGQ
                     TVVDNLFGSMDPLGQVIRIRKIPFTVIGVLESKGQSLGGQDQDDTIIVPLSTAQKKLF
                     GRTIPGMVRSIMVKARSSEELSVAERQITDLLRQRHRIGPNREDDFTVMNLTQVLEMA
                     EQTANTFALLLGAIASVSLVVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRIQF
                     IVEALTLSMIGGITGIIIGGTASFILSNVFEIPSVVSLPSVLLAFCFSGLVGIFFGFY
                     PAYKASLLNPIDALRFE"
     gene            7828..8331
                     /locus_tag="PLG98_05020"
     CDS             7828..8331
                     /locus_tag="PLG98_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32647.1"
                     /translation="MRKIFAIFISLFFIASLTLAVTGCSKIEEDSANLQKAAAELKAQ
                     AEAKKAEAEMKAQADAQQAEADLKAQAEAMKAEAELKAKEMAKKATVKVKQVKKDVEK
                     VANKVANKVATDVKSAGQEVKEAAKETVVDVKSAGTEAKEAVKEKAVEVKSAVNDAAT
                     DVKNAGQ"
     gene            complement(8409..10151)
                     /locus_tag="PLG98_05025"
     CDS             complement(8409..10151)
                     /locus_tag="PLG98_05025"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOE32648.1"
                     /translation="MDVRRTINALKDVNIAKMFTTIARLLHFRLTWTRRDLDYLPAGL
                     GPKFVSARRAAAMIPDGATLTIGGFAATGRASIFYWALRDAFDRSGHPRNLTVIGACP
                     QGGRGKTPGMIEELDAPGIITRYIVGHGETAKALLQLADDGQLELHTMSQGALAFLIE
                     AQARGLASIQTSVGLDTFLDPAVGCGSAVSPGAPNNFITAKNGKLEYRIPPIDRALFL
                     APYADKDGNIYFKDAALILDYKDAAAAARLNGGKVIVSVSKIIPRDNSQIAIPADNVN
                     AIVVNPRNEQVGGILQTQFMPHFTAGSSELDHEAVKLMRLINTFLDITPYRGKVVDIL
                     ARLCADTFVKTTGPGAIVNIGIGIGEEVARMLYESGLYRDITFTSEGGAYGGLPASGV
                     YFGAAISPQRILSTAEIFHIYEERLDTCVLGFLQVDSAGNVNVSHRGPKIIDFVGPGG
                     FTDIVEYARTIIFVGNWMDEARYGLKNGLVRVKKPGVPKFVDKVDQITFNARKALKAG
                     RKVLYVTTVGVFKLTEEGLELVQIMPGINIEKDILQNSTARFIIPGSPIPEVAPEIVS
                     GKRFELKFDKKDLA"
     gene            complement(10218..10505)
                     /locus_tag="PLG98_05030"
     CDS             complement(10218..10505)
                     /locus_tag="PLG98_05030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOE32649.1"
                     /translation="MITLIATLKVKDGNMDEAIQVLKEIVPQVKASEPGCLDYIPHTV
                     RGDDKTIIFYERYSDKDALKLHSANLMKNMEKLFPLLEPGMDVKTCYEIIR"
     gene            complement(10655..11125)
                     /locus_tag="PLG98_05035"
     CDS             complement(10655..11125)
                     /locus_tag="PLG98_05035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOE32650.1"
                     /translation="MAEKTIIILGKSVKNFTLADQFGQDFMLSRMTGRRVLLSFHPLA
                     WTPVCTRQMQSLEKNKKVFDKLNTIAVGLSVDSVPSKAAWAKAIKVKHTKLLADFWPH
                     GGVAKSLGLFRDQNGFSQRANVILDEKGKVCFVTVYPVADLPDINEIIAFLKNA"
     gene            complement(11187..12572)
                     /locus_tag="PLG98_05040"
     CDS             complement(11187..12572)
                     /locus_tag="PLG98_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOE32651.1"
                     /translation="MSPESISQTLTGLRAFFDAGKTQDVDARIARLKKLKEGIRRYEP
                     EIMASLYDDLRKPLFESYAGEIGILYTEIDHVIRHLPSWSRSRRAATPLIHFLSRSRI
                     DQKPYGVVLIIGPWNYPFQLVMAPLIGAIAAGNCALIKPSEFSPATSATIEKMISELF
                     DPGDVTVFQGDAQATQKLLEEKFDYIFFTGSTAVGRSIMTAAARHLTPVTLELGGKSP
                     CIVHEDAALQYGIKRIAWGKFFNAGQTCIAPDYVLIHRKIKADFTEKMIRTVFSFYGD
                     DPMRSPDYARIINERHFNRLLELLKSGRILFGGRYARKDLYISPTIIDRVSPEEPLMQ
                     EEIFGPLLPLIEYGDIDEAIAFINQRPQPLALYCFSEDKEIQEEVLQKTSSGGGCIND
                     TISHIGSQELPFGGIGASGMGAYHGKAGFDTFTHRRGILFRSNLVDIPLRYPPYRDRV
                     SILQLLFRLIR"
     gene            12828..13352
                     /locus_tag="PLG98_05045"
     CDS             12828..13352
                     /locus_tag="PLG98_05045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE32652.1"
                     /translation="MRRIFHDPVTILGSYVRRGDTAVDIGPGMGFFTIPLAKLVGPAG
                     KVIAVDIQPRMLSALAARAQKHGVAAIIRTHLAEPDSLALREKADFILAFWMAHEVPD
                     LKKFFSEIRKLMKPQGWFFLVEPLAHVSRKDFLQTIDLAKQSGLLMKECPKIRMSQSA
                     LFTPDKIERHEQPL"
     gene            complement(13594..16848)
                     /locus_tag="PLG98_05050"
     CDS             complement(13594..16848)
                     /locus_tag="PLG98_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32653.1"
                     /translation="MIEIDMRIIFAEYGLSVLLCLIVIVSLWWQNRKRAPEIILWLID
                     YILQFAGIMLIALRGILPDFMTIVVANLFIVGGTVILYEGLCRYVGQESRQKHNYVML
                     AIFTSVHIFFTYIAPHMGLRTINFSVALFYICIQGAWLMLYRVASPLRQATKITGIVF
                     AAFCIVNIIQIIINLIKTPSDNAFVSGAAGVIAVLIYETLFFALTFSLFLLISRSLAI
                     ELENELTRRMRTAEELRQNQEKFAKAFQTSPYAISITQMKDGKFLDINDAFVNITGYS
                     REEIMKESSMGLNLWVDKEDREEMVSILRQGLPVVRKEYRFRKKNGEILTGLYSSQII
                     NITEEPCLLSSINDITEQKQAEWTLKERIKELNCLYGISAVLDEPGISLNDIMNKAVK
                     LLPAAMRFPDITGARIILEETVFQTGLFKETPWQLTEKITVNGKPEGQVNVCYPAERF
                     QTDEKPFLDSERQLLKAVAEQLGHFIEQKRAEEQIQRNATRLTGLLNIMQHRTQTTQE
                     FLDYALDEVIRLTESKVGYIYLYNENSRQFVLNSWSKDVMKECTIPNPQTRYELAKTG
                     IWGEAVRQRKPIIVNDFPADHPLKKGYPEGHAPLFKFLTVPVFDQDQIVAVVGIANKA
                     RDYDETDVLQLTLFMDAVWKSVDLKMGEAKLRKSEERFKQLAEVFPETIFEADAKGNL
                     TYANEHGLKHFGLTEQDIAQGVNIFDFVSPNDRKAAWEKIQNRIQGTQKGYLEYEALK
                     KDGSPFHALALSVPIAVDGVPVGIRGFILDITKRKQAEEALRKSEENYHFIADHTADH
                     IWTMDMFMRFTYSSPSVIKTLGYTADEFMARKIDEFFTPESLVDAQKLLAEELEREND
                     PTADPNRSRTLPSHLRHKNGHWVWLESSLTFIRDASMKPIGVLGVTRDITERKKSEEQ
                     IKHLATHDLLTDLPSLRLARDRMVSAMNMARRYKKSAAIMFLDLDGFKNVNDTLGHDA
                     GDYVLKQVAQRLLSCVRETDTVARVGGDEFLIIATEIHHPDNAAHIAEKVIQTVSQPI
                     MINGEQAVVGTSIGIALYPEHSEEMDRLIKLADEAMYKVKNAGKNNFRFVNDVN"
     gene            complement(17184..18728)
                     /locus_tag="PLG98_05055"
     CDS             complement(17184..18728)
                     /locus_tag="PLG98_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HOE32654.1"
                     /translation="MTAPLIIAMSQGKEITLLPQMANRHGLITGATGTGKTVTLQKMA
                     ESFASIGVPVFMADVKGDLSGIGAAGVASEKLLKRLEGIGVKDYQPRANTTVFWDVYG
                     KSGHPVRATISDMGPLLLARLLNLNDTQSGVLQIVFKIADDNGLLLIDLKDLRAMVQF
                     VGENAKKFKTEYGNVSAASIGAIQRGLLTIEEQGGDKFFGEPMLDISDLIQTDSEGRG
                     VMNILAADQLYNNPKLYSTFLLWMLAELFEQMPEIGDPEKPKLVFFFDEAHLLFSDAP
                     KALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPDTVLAQLGNRVQHALRAFTPRDQKAV
                     QTAASTMRQNPAFDAEQAITELGVGEALISFLDEKGRPNIVERCNVIVPFSRMGAMTP
                     DERLAAIKASTIYGHYEKTVDRESAYEKLREAQQEEETPEEKPAIKVERSAAPAKSEE
                     EKDSGGMGDALSKMIFGSKGPRGGQREGMVQAAAKSVARSIGSSLGRQIIRGVLGSIL
                     GGSRGR"
     gene            19083..20306
                     /locus_tag="PLG98_05060"
     CDS             19083..20306
                     /locus_tag="PLG98_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOE32655.1"
                     /translation="MDLQISAGDTGWILVCCSLVLLMTPALAFFYGGMVRRKNVLSTL
                     TLSYIFMSLIGVQWVLFGYSLSFGPDTGGLIGGLDYIGFQGVGAEPNAAYSRTIPHEL
                     FAAFQMMFAVITPALITGAFVERVKFKSFLMFSLLWATLVYNPLCHWVWGQGGWLKQM
                     GVLDFAGGTVVHIAAGFSALAFALVIGPRKGHGSVPMEPNNIPLTVLGTGLLWVGWFG
                     FNAGSALAANGVAVNALVTTNTSAAAAGLVWMLLSWLDGRPSTLGIATGMVVGLAAVT
                     PASGFVTPLAAMVIGAVSAPLAYYAIRFRERRKLDESLDVWACHGMGSTWGTVATGLF
                     ATVAVNADGANGMFFGNAGQLAIQITAVLVTILFSFGMTYILAKVLHVSIGLRVSPME
                     EEVGLDISSHGERSY"
     gene            20367..20744
                     /locus_tag="PLG98_05065"
     CDS             20367..20744
                     /locus_tag="PLG98_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOE32656.1"
                     /translation="MLKKIEAIIREDKVNDVMCALKEIGIVGMSMFEVRGHGRQGGVQ
                     LVGRAGTYQVNLLPKIQLNIVLSERNLEAALETILKAAYTGETGDGIIFVTPVEDVIR
                     VRTRERGEGAMIYPGDIDERRKK"
     gene            complement(20986..21546)
                     /locus_tag="PLG98_05070"
     CDS             complement(20986..21546)
                     /locus_tag="PLG98_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32657.1"
                     /translation="MNIKGSSFVTTKAIITEAFGEERWNAFHAGLTQKDGFFNNMIMS
                     VTLIPVEKMIVFFNELCREFFNNDKTQYAMFGKSGAKFVLSPTGPYKSYMLAKDARQF
                     VELNMPKIWSTYFDGGTFTSNFDNNTAHITITGIPVKNNYFEELVMGFNQQAFKIFGE
                     KTTAVKVSSIAAGDDDFRFQLKLKDS"
     gene            21833..22252
                     /locus_tag="PLG98_05075"
     CDS             21833..22252
                     /locus_tag="PLG98_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOE32658.1"
                     /translation="MKISTRTRYGVRLMTALARNYGKDPVFLKNIAKEENISEKYLSL
                     IIIPLRGGGLVNSVRGAYGGYNLNKDPSEITLKEVVDVLEGDRFLVDCVNNPAACPRV
                     TTCASHDVWAVVGEKISETLSSITLDRLAKMNQKKEK"
     gene            22325..23386
                     /gene="mnmA"
                     /locus_tag="PLG98_05080"
     CDS             22325..23386
                     /gene="mnmA"
                     /locus_tag="PLG98_05080"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOE32659.1"
                     /translation="MGDKVLVAMSGGIDSSVAAWMLLNEGYNVAGVTMHFGALTEGDG
                     KRSCTSAAIDDAKKVCACLNIPHYVFDYASRLGNEVIADFISEYQKGRTPNPCVRCNR
                     HLKFGSLLDKAQELGFNYLATGHYAAIEKNEGGYCLTRPKDKKKDQTYFLYAIPYERL
                     KNILFPLAQLTKDEVRNLAMKNSLPVTAKQESQDICFVTQKNSQTFLLERVKDIAPGP
                     IVDVRGNVLGRHRGIIFHTIGQRGGLGISHPTPLYVLSIHPGNNRIVVGDKKDLMGKA
                     LIASGINRLAKSWPQQMHAKIRYRKKEALCEVREEKEKLKVIFAEEQEAITPGQSVVF
                     YDHDCVLGGGIIEKVIPFG"
     gene            23414..23848
                     /locus_tag="PLG98_05085"
     CDS             23414..23848
                     /locus_tag="PLG98_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOE32660.1"
                     /translation="MKILAVNISQKKGGKKNNIGCGLFLENLGLESDAHAQADIIRQV
                     SLLAKESIEKIRAKGLDVQYGDFAENLTTEGIDLPSLPVGTRLQAGANVLLEVSQIGK
                     VCHNRCNIFYTVSDCVMPREGIFAKVLIGGEIKINDEIQISK"
     gene            24089..24907
                     /locus_tag="PLG98_05090"
     CDS             24089..24907
                     /locus_tag="PLG98_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32661.1"
                     /translation="MKENAVLNKILKAVRIGERSGAKVVGLGALMGVAGKGGQRIAEQ
                     TPAAITTGSSLASAAILETLDRAAAMRNVDLSKAKVAIVGATNAIGQNCALGFLNRVD
                     TVFLLARNADRLAELKEFLNAQKSKTNIIDQGPALEDVITEADLVIFTTSAIEVPSAA
                     TAKNLKKGAIICDIPSPRNIAREICDQRKDILVIDGAVIEPPPTAQLGLKLPIQDGYI
                     YACMAETMILAFEGQTQDDFSTGFRPDLHKVARIKALAAKHGFNIKFTSFGVPV"
     gene            25115..25423
                     /locus_tag="PLG98_05095"
     CDS             25115..25423
                     /locus_tag="PLG98_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32662.1"
                     /translation="MDDVITRIVDIEKQCSADVEATQLEYGKNIEAHKRFLEAKKTRE
                     RAEILSAENKRQSQAVEDAKRNIEAVSADFITDMENLFRDQGLNEAIKKDIVSILLGG
                     "
     gene            25429..26379
                     /locus_tag="PLG98_05100"
     CDS             25429..26379
                     /locus_tag="PLG98_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOE32663.1"
                     /translation="MRNYADQANLHARIYAMKGRLLSLQDYAVMAREQRTFAPKSSSV
                     QDLIDAKENLFREQIAPVIALVEAYDKYTPVFLAYLRQFETHNAKVLLAKAAGKDILE
                     QWYDISPFASLDKSLLNKNLSMDDVTSLLTNTYLGADFKPSSSYRRMQMNLDISSARN
                     IYNASSLISDQARKEFQEMILRRIAVQMVIRSYRLRVYYHWSEKDTRLYLEKINNRLN
                     IHAESQIHSIDEDLNQHIETLRKSSGQEPSVIDIEQHLEQIYYLWLSSIFYRDFHSIY
                     GVIAYLGLLLIQIKNLFRIIDGRRFGFSADAILSKIICKE"
     gene            26388..28274
                     /locus_tag="PLG98_05105"
     CDS             26388..28274
                     /locus_tag="PLG98_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOE32664.1"
                     /translation="MFLKSDIRHITIALEKAYYSDVYLALGQAGIIHLARFSEKDSGT
                     DSGLQKEEALTREIISNTEYALNALMIAPEESEIFMPAVDKSGDLIFVSRIKKTIEQA
                     VRLRAKIREKENIIIRQMGYAEALSKMAIDPVAIKKARLVKILFGTVENMTDDVPPNS
                     RFMLNKINNYVLGICLPDDYPALFRFLKNYGFIDLSDDVTPVSLESLKKRREGLKRRR
                     DMMDAYLDRLKTEQGRWLQNLNKSYHHYEEILKAMRMSLFSAKALFITGWIDIRNKQR
                     LLKILEKICGNRFIFSERKDPDAPVHLRNMKLFKPFELIVKTMGIPSNREIDPTPLTA
                     ITFVLMFGLMFGDLGQGLVLLLCGIMLKKYGQKKIKENLKQAGGILVACGFSAAICGL
                     IYGSVFSSEHLLPALWFHPTANIMTLFAATILIGILFIMTGLLVNIINNILNADYTEA
                     LLDKRGLVILVIYAATVLFAFNYQRSGQVPAIWEISAFIMLPLTLFALRGVIGPAFFH
                     QESPHSASEYIIETVMEIVETGLSLFANTISFIRVGAFALSHAGLSIVTYTLAGIADP
                     GLQSAGAILIIITGNIFIIGFEGLICGIQSMRLEYYEFFSKFFQGDGVVFSPFTLKAQ
                     ASEV"
     gene            28276..28611
                     /locus_tag="PLG98_05110"
     CDS             28276..28611
                     /locus_tag="PLG98_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOE32665.1"
                     /translation="MIKNKLFKHFVFAVFTGFIWFALLPTVINAAENVQVNSGANWMG
                     LAYIGAGLAVGLACIASGFAVARIGSAAIGAVSEKPELMGRTLVFLGLAEGIAIYGLI
                     IAIMILNKL"
     gene            28824..29129
                     /locus_tag="PLG98_05115"
     CDS             28824..29129
                     /locus_tag="PLG98_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOE32666.1"
                     /translation="MEKIYIIGDMHTVSAFRLAGVEGMVSTADSASVRLEEIIKKRDA
                     GIVLMTNDLAAALQERIREINLNRQNPVVIEIPGMDDIGGLRMSAMNYISEALGIRL"
     gene            29139..29744
                     /locus_tag="PLG98_05120"
     CDS             29139..29744
                     /locus_tag="PLG98_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32667.1"
                     /translation="MDNTTLENSIREEASRAITAIREKEALEMRQRDEVFSMEMEDFR
                     RQAEAETESHIRQEISKLENKALLERKKMKLQNAEQFINRIVDEVMKNIRNDRLYKQF
                     LLEAIRDGVKISGADVDIKLSNEDIFLKKDIVAAIAAMDGRKKVSITEDSKVQWGGCL
                     IRDKETGRIFNHTLERIYFRKSLLIRQQVMSLLMRKAEGNQ"
     gene            29770..31533
                     /locus_tag="PLG98_05125"
     CDS             29770..31533
                     /locus_tag="PLG98_05125"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOE32668.1"
                     /translation="MDEILKGNVISVNGPIVKVVGLTGIKMFDIAEVGQERLIGEVIR
                     LAGEITIVQVYEDNTGLKPGDDVISYGRPLSVLLGPGLIANIYDGIQRPLVGISEVCG
                     SYIKKGIKLPPLDVNKKWDFQPRVKTGEDIKEGTMLGEITESPLVVHRVLVPPGIAGR
                     LTAIVNAGSYTIEDDIFEIDTGSGKYNGKLAAYWPVRKPRPSRNRKKPFMPLITGQRI
                     IDTFFPIAKGGTAAIPGGFGTGKTMTQHALAKWCNADIIVYIGCGERGNEMTDVLTDF
                     PKLIDERNGRPLIERTVMIANTSNMPVPAREVSIYTGVTIAEYYRDMGYSVAVMADST
                     SRWAEALRELSSRLGDMPAEEGFPPYLGTRLAEFYERAGLVETLSGSNGDITIIGAVS
                     PPGGDFSEPVTQHTSRFVRCFWALDKILADARHYPSISWIDSYTEYLVDIEDWWKNLD
                     SEWLTTRNEVMNILLEDHRLEQVVKLVGSDALPPSEQYILFCAEMIKNAFLQQNSFDS
                     QDKFCSPEKQLKLLKCLLVLYQKGMTLIQEGVPVKDISQSDVVSEMVRLKSAVPNKEL
                     VEIDDYLKRLEQELMSIKSSS"
     assembly_gap    31713..31812
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31813..33090
                     /locus_tag="PLG98_05130"
     CDS             <31813..33090
                     /locus_tag="PLG98_05130"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOE32669.1"
                     /translation="GDLRIGQTLEVDEQRAVVQVFEGTSGLSVKETRVTFVGRALEIP
                     VDREMLGRVFDGLGRPVDGAPQVITDRMMNVNGLPINPWSREYPRDFIQTGISAIDGM
                     NTLIRGQKLPIFSGSGMPHNRIAAQIARQARILTAEEEFTVIFAAMGVKFDVARFFID
                     SLEESGVLTHSAIFLSLADSPSIERLVTPRSALTLAEYLAFEEGMHVLVILTDMTNYC
                     EALRELSSSRGEIPSRKGYPGYLYSDLASVYERAGRLKGGKGSITQMPILTMPSDDIS
                     HPVPDLTGYITEGQIVLERELFNKGIYPPIAGLPSLSRLMKDGIGEGRTRKDHGQVAA
                     QLFASYAKVKRIRALAAIVGEEELSDLDKIYLKFGTAFEEAFLRQAYHENRSIEQTLE
                     IGWKMLSLLPRDELYRIAREDIQRYYMSETEKT"
     gene            33090..33710
                     /locus_tag="PLG98_05135"
     CDS             33090..33710
                     /locus_tag="PLG98_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOE32670.1"
                     /translation="MPKIELPPTKSSLRKLKEDLAFAYEGYDLLNQKREILAIEIVRK
                     IDVIRKIEAEFQDIVNRLYLAYRQAAVDMGSEAVTLQSCSEMRSYYLHMNLTKLMGLK
                     LLNFTLDLKNNLPAFTLPPTTSAYDNAKEQSRKALHIVVQYATIMKSVIMLSRELKKV
                     QRRVNALEKIFIPQHEEAKKYITDRIEEMEREEIFVKKLIRQRQQR"
     gene            33749..34273
                     /locus_tag="PLG98_05140"
     CDS             33749..34273
                     /locus_tag="PLG98_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HOE32671.1"
                     /translation="MKSKSDVLKYLELAERQARDKKKKSNMKTYAYKYLELAEKHAEK
                     IAARWVKDVRNNTRTQTYKKLNEKQLVDQCTEFYQQFSKMFIYEKITDDVTKYFQKYA
                     LECYALDIPAKEAIYALILMRRNIWLYAEFQMIFNSAIAKQESLDTLNRTILLFDYAV
                     YEVTREYLELMKTT"
     assembly_gap    34591..34690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(34766..>36310)
                     /locus_tag="PLG98_05145"
     CDS             complement(34766..>36310)
                     /locus_tag="PLG98_05145"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HOE32672.1"
                     /translation="REEDLIAREPDAEEISQYPDRIKIGDAAFNCRYNFEPGKNDDGV
                     TVKVPLGFISKAATANIDRHLPSLLKEKAVHLLKSLPKSIRLKLPPPAKIVELLVEDK
                     SNLDKPLPQALSQLLLDKYKIKVPKDDWATEKLPDYLNVRYSVIDEKGNEIKESRDIH
                     LLQKELTGTVSTSAMDKIRGEWEKEGITGWDFGDLPQQIPLRGTHGLIGYAYPALTIS
                     DDSIDLRLFADEKQSAFHHLHGIAALYDIHFADKMKQLKKNVALTGAMKTSATGIGPP
                     KQLEQSIINRVRKDLFYKPWRRQEEFIAHAGCLNSQILPYGQQVLIAIEPVLKACADT
                     CTVIGKLMTKNRNNNPVLNFIKDIQTEISRLVPVDFPELYTFERMKELPRYCKALALR
                     AERGNLNLSAAQRKMQDVLIYSQKLQQMVNGRVQPSLFDLAVVKKQSSPKDPTEPKGT
                     GFKEIILLKENITIDCPDEKKTMVEEFFWMIEEYKVSLFAQELKTPYPVSPKKLNQLM
                     EDIEKY"
BASE COUNT         9500 a         8273 c         9131 g         9206 t
ORIGIN      
        1 taccgttaaa attaacgacg attatgagct gggtcaacaa atgtacatct gggaaatggc
       61 gaccgcggtt gcggctcatt ttatgggtgt taatcctttt gatcagccgg atgtggaagc
      121 gactaaaaaa catacccgcg ccgtgattgc cgatttagac aagcgtggag aattggcgga
      181 tgaaaaaccg gcaataacct ttgaacaggc tgaaatcttc ggcaatatta acggtgcgac
      241 tccggcagac gttatccgga attttatgaa acaggccaag gcgggtgatt atatctgtct
      301 gcaagttttc ttgattccgt ctcaggaaac gaatgaggca gtcaatagtt tacgtaaagt
      361 tcttgcatgc caatatactt tgccggtggc ctgcggatac ggtccgcgct atctgcattc
      421 caccgggcag ttgcacaagg gcgatgccgg aaacggcttg ttcattcagt tgacgcagga
      481 tttttcttta gacgttgatg ttcccgacag catcggcgaa gaaaaatcct ttctcacttt
      541 cagcgcgctc aaagccgcgc aggcgaaggg cgaccggcag gcgctgacgg aagcagggcg
      601 aagaatcatc cgtatacatt tcaaaaaaaa tccagtcgat gggttaaaaa caatagtcaa
      661 tggtctctaa gcaaatctca atattggcga gttattcagt tgtcaggctg gagcgggcaa
      721 tccagttgta taaagacctg gccgcccgct tacgcgggaa cggcgcctgg attaccgcct
      781 gtgcgggaat gatacattat ttcatttaac tgatcaccct tagaatttcc ggcgcgtggc
      841 gttcggatac cggcggaatt gtctttggat aaccgagaat aatcggcgcg acaatccggc
      901 agccgtcggg aatggccagc agggccctgg actgaggatc ccggatgttg gatcccagat
      961 tgacccagca ggttcccagt ccgcgctcga cggcagaaaa cataatgtag cttgccgcca
     1021 gcgcgcaatc cacgtcaagc gacttgacgc tctttgaacc gatgatgaaa atcacgcagg
     1081 gggcgttgta gaagacattg aaattttcat ctttcagcat atcgatgtac tgcggcagga
     1141 gggatttttt attgccggcg taatcctcca ggagatttgc tttgctttca tcggaaagcg
     1201 ccttgatggt ttttctgcaa tggacgatgg aaaaccggca gggctgattg ttgctggccg
     1261 aaggcgccaa cgtcgattcc tgaaggattt cctcgataag gttcatggga acgttcttgt
     1321 cctgaaaatc gcgaatggac cttctgtttt ttaataaatc cgtaaaggtc atcatggtta
     1381 ttacctccgt tgttaaaaaa ctgtccggtg atcatccagc cgactcgcgc ggtaatgcgg
     1441 agtcggctgc tggcttcact ttttctatat catgaaaata gccgtatttt aaacctatta
     1501 ttattgcggt aacagccatt aaaagttcaa caagggttat gagaaatcgg gagatcaccg
     1561 ggaggatcag ggccgcttca atattggaaa actgcttcag catgaaaaac atggctcctt
     1621 cccgcacgcc gagtccgccc acggtaaaaa aagccagcaa tccaagaatc agcgaaacgc
     1681 agattgtcgc catcatggca atgatctgcg aaaacggtaa tggcatgttt actccccggg
     1741 caagaaaata cagggcgatc ccgagcaaaa cacacgccag cgagtaaagc atctgtacat
     1801 agacaaaaat gattttgtcg gttcgaatcg gtttgatgtt caacttgaac aatcggttga
     1861 tgggccgaat cagataattg acgatggtat ggttcgcgat aatgaaaaca acatccatca
     1921 gcataaacag cgttaagatc agcagagttg ccccgaggga catttcctga acgtaaatga
     1981 aatagtagta caagaagaac ggcacggcga cgaaaagcag acagataaag ctgacgagat
     2041 tgatataaag caatgcgacc ttagaaattc ctttggatga aagcagggct atctgagccg
     2101 cataggtcca tatttttccc gggatgtatt tgaacatggc ggatgtgcag taaagcacaa
     2161 agctttcagt atagtttaat tttttcttca aatagctgtt gacaaaaagt cgccagacga
     2221 gaggtccgac aagcagggcg caactgccga aaattaccgc caggctcatc tccagggggt
     2281 taatgctgaa cttccaggcg ccgatggcgt cggcgttttt tttgaattgc aggtaaaaaa
     2341 agtaaactgc agcggcgata accgttaaag cggaaatata tcgcagcatg ttttttgtat
     2401 tcactccgtt gtctccgtct aaggccgggc aggtgattga aatccgctta actgccggac
     2461 gttcaagaaa attttttaag cggtttaact gtatatgaca aataggcgct gttgtcaaaa
     2521 aatgccccga aaaacgggac gttgatcgtt tttgtgaaaa aaaccttgac aaagcggttc
     2581 aatattcata cgcttgttta aaacaggtgt ttgaaaaatg gttttagcct ccgggaaaac
     2641 aaattcaaaa acacgcaccg cgatcattga ggcggcaggg aaaatctttg ccgaagaagg
     2701 ttacctcaag gcgaccgtcc gggatatttg cagcagcgcc ggggccaatc tggcggcggt
     2761 caattattat ttcggggaca agaagggatt gtatctgtcc gtgttgaagt attatcagga
     2821 gatggctttt caggcgtatc cgcccgatct gggtatccgg gcctctcaga aaccggagga
     2881 aaagcttaaa atttatatcc ggatgtttct gaaacggatt atggatgacg gacgtcccgt
     2941 ctggttcggc aggctcctgg cgcgggaatt cagcgaaccg acctgcgcgc tggatgttct
     3001 ggtcaacgag accatccgtc cttctttcca actccttacg gaaatcgtcg ccgacgtgct
     3061 gggcaaaacg ccccgggaaa aaatggtgcg gctgtgttca atgagtattg tcggtcaatg
     3121 tctttatttc cgccattcgc agccgatcat gaccagaatt tatccggaag caaccttcgg
     3181 ggcggaacag atcgatgaaa taaccgaaca catcgcctcc ttttccatag gaggcctgag
     3241 gaaagcgcaa taatcagcca gggtgaaaaa atgcaaaaaa agataatgct ccttttttta
     3301 atcgtcttga ttccgtttgc cgccgccgct tcggagccgg caggtggaac ggcctcgtat
     3361 gatcttgatc agtgcattgc cgttgctctg gacaagcatc ccagcctcaa ggcgtccgcc
     3421 ggcgacatcc gggccaggga aagccgggtc gggcaggcca ggtccaaata ctatccgtgg
     3481 ctggatgtgt ccgccggcta tcagaagatc ggtccgacca cgccgcccgg cgattccatt
     3541 gattcgtacg atcagtattc gacggggttc aatctgggcg tgaccctttt cgatttcggc
     3601 aagacgtcag caaatgtcgc cattcaggat ttaaacgtaa acgcgtcgcg cgccgattat
     3661 gacgatgtca aagcgcaaat cattctgaat gtgaaaaaag cttattacac gcttttgcag
     3721 aaccagcgca atcgggatgt ggccgtcgat acgatgcagc agttccagca gcatctggat
     3781 caggcaaacg cttttttccg cgttggcacc aaaccgaaat tcgatgtgac caaagcggaa
     3841 gtcgatttag gcaatgcgcg gctgaatgtt ttgaaggcgg aaaatgccgt tcgtgctgca
     3901 cggatcacgt tgaaggacgt catgggcatt ccggaaaacg ccgattttac aattgtcgat
     3961 aacctgtctt ttcagaaaca ggcatggatc ctggacgata ttttgaacaa ggcgtatgcc
     4021 gtccgtcctg atcttctttc gattatagcc agacgcgagg cagccgagcg gtcgatcgat
     4081 ctggcgagaa aagattatct tccgaccctg tcggggagcg ccggatacgg cttttccggc
     4141 cgtgattttc cgctgggccg cggctggaac gcgggcgcgt ctctcagttt tccgcttttt
     4201 tcggggtttt cgaccaacta tcagattgac gaagccgaag ccaatctggc aatactgaca
     4261 gccaacgaag agaccgtccg ccaggccatt cacttggatg ttcagcaggc ctatctgaat
     4321 gttcaggata ccgctgaaca aattgccatg gctgaaatga cggtcgttca ggctcaggaa
     4381 aattacgatc tggcgtcggg gcgttatcgg agcggcgtcg gcagtcccat tgaagttgcc
     4441 gacgcgacga ttatgctcaa taacgcgaga gccggtttaa atacggcgct ttgtaattac
     4501 aggatcgcac aggccgttct ggaaaaatcc ataggagcaa aaccatgaag aagaaaatca
     4561 ttgtcatctc cgtattgctg attgccgcct ttgcggccgg gatattattt tatgtcaaca
     4621 gagacgggaa aaaagccgtc tataaaacgg aaaaaatttc gcgcggtgaa gtcaaatccg
     4681 ttgtgaccgc taccggcacc gtgaatgccg tcacgactgt ttctgtcggc acgcaggtat
     4741 cgggaaccat cagaactctt tttgcggatt tcaattcgcc ggtgaaaaag gggcaacttc
     4801 tcgcacagat tgatccggcc acgttccggg cgcaggtgga tcaggccaga gcaaacctgc
     4861 tttcggccca ggccaatctg gagaaagcgg gggttgccgt cgttgacgcc cggcgaacct
     4921 acgaacgaaa caaggaactt ttttcccaga actttatcgc acgcagcgat ctcgatacgg
     4981 cggaaacggc tttgcatgcc gcacaagccc agcagaaagt cagcgaggcg caagttgcgc
     5041 aggcgcgggc ctctctgaga atggcggaaa ccaatttaca gtacaccaat attatttctc
     5101 cggtgaacgg cacggtgatt tcccgcagcg tagatgtcgg ccagacggtg gccgccagtt
     5161 tccagacgcc cacgcttttc agcattgccc aggatctcac gcaaatgcag attaacgcca
     5221 gtgtggatga agcggatatc ggtcagatcc gggcgggaca ggacgtctcg tttacggtgg
     5281 atgcctatcc ggacacggac ttttccggca aggtttccga ggtgcgcaat gcgccgacca
     5341 ccgtctccaa cgtggtgacg tacgacgtca ttatcaaggt ggataatccc gagttaaaat
     5401 taaagccggg gatgacggcc aatgtttcca tcaccataga aacgcgtccg gatgtcctgc
     5461 gggttccgaa tgcggctctt cgttttaaac ccgcggaggt cggggaaaaa ggcgtatcag
     5521 cgaaagagct gcaggacatc aaagggacga aagtctgggt tctggaaaac aatcagccca
     5581 aacccgtcgg cgttacgctc ggattgagcg acgggaattt ttctgaaatt gtgaaaggcg
     5641 acctgcgcac gggccaggag attgtcacgg acagcctgaa taaaaaaggc agcagcacca
     5701 cacagcgccc gcctccgagg tttatgtgat gactctgatc gaaacaaggc agcttgtcaa
     5761 aacgtatgat gtgggcgatg aaggcgttca cgcgctggaa aacgtctccg tcaaaatcga
     5821 caaaagtgaa tttgtcgcca tcatggggcc gtccggttcg ggcaaatcca cctttatgaa
     5881 tatcatcggg tgtctggatc agccgactgc gggggattat tttctggacg gacaaacgat
     5941 aagcggtctg tcacgggacg atctggccgc gatccgcaat cagaaaatcg gttttgtctt
     6001 tcagggattt aatttgctgt ccagaacgtc ggcgctggaa aacgtcgagt tgcctttgct
     6061 ttataatcac gtcccggcaa ggcaacgcaa agaggaatcc ttaaaagcgt taaaaatgct
     6121 gggtctggag gaacgggcgt atcaccatcc caatcaactg tcaggaggac agcaacagcg
     6181 cgttgccatt gcccgcgcgc ttgtgaatca ggcgcctata ttaatggccg atgaaccgac
     6241 gggcaatctg gatacgaaaa ccagcattga aatcatggaa cttctggttc agttgaaccg
     6301 ggattcaggc acaaccatta ttctggttac acatgagccg gatatcgccg acttcagtaa
     6361 acgcatcatc cggtttgttg acggacatgt ggtcagcgat gaggaggtgc aaaaatcatg
     6421 atcagtattt tttctacact ccgcattgct ttaagggcgt tgtgggtcaa caaaatgcgg
     6481 tcggcgctca ccatgctggg catcatcatc ggcgtcagcg ctgtgattat catgctggcg
     6541 gtgggcacgg gggtcagccg gaaaatatcc tctcaaatct ccagtatcgg cagcaatctg
     6601 atcatcatca tgccgggcag caccacgcag ggaggcgtca gaatgggtgg cggaagtcaa
     6661 tccacattaa ccaccggcga tgcggaggcc atattgcggg aatgttcagc ggttgccgcc
     6721 gtggctccca tgcagttcgg caccgcccag gtggttttcg ggaatcagaa ctggtctacc
     6781 ggcattcagg gaacgacgcc ggggattctg gaagtcaagg actgcggttt gtcggcggga
     6841 agaaacatgt ccgatcagga tatccgcagc gccacaaaag tatgccttct gggacagacg
     6901 gttgtggata atcttttcgg cagtatggat ccgctcggtc aggtcatccg catcaggaaa
     6961 attcccttta cggtcatcgg ggtgctcgaa tccaaaggac agtctctggg cggtcaggat
     7021 caggatgata caatcattgt tccgctaagc accgcacaga aaaaactctt cgggagaacc
     7081 attccgggaa tggtgcggtc gatcatggtc aaggcaagaa gcagcgaaga actatccgtg
     7141 gcggaaaggc agatcacgga tctgctgcgt cagcggcatc gcatcgggcc gaaccgggaa
     7201 gatgatttta cggtcatgaa tttgacgcag gtactggaaa tggcggagca gacagccaac
     7261 acctttgccc tgctgttggg tgcgatcgcc tctgtctcgc ttgtggtcgg cggcatcggc
     7321 atcatgaaca tcatgctggt ttcggtcacg gaaagaacgc gggaaatcgg cattcgcatg
     7381 gctgtaggcg ccaagacctg ggatatccgc attcagttta ttgtggaggc gctgaccctt
     7441 tccatgatcg gcggtattac gggcatcatc atcggcggca ccgcttcctt catcctgtcc
     7501 aatgtttttg aaatcccgtc tgtcgtatcg cttccatcgg ttctgctggc attttgtttt
     7561 tccggtctgg tgggcatatt cttcggtttc tacccggctt acaaagcctc tttgctcaat
     7621 ccgatcgatg ctttgagatt tgaataagca gcatatcggg aggccgaggt tttcatccag
     7681 atgtataaaa acggacaggt ccgttcctga tgaaaattct tatttttgaa tcttgacaaa
     7741 gctccctgcc gcgtatagcg ttccatcgat acaaaaactt ggaacatgaa gccaggttct
     7801 aaattcttga aaggaggttc ccagttgatg cgaaaaatat ttgctatttt catttctctg
     7861 ttctttatcg cttctttaac tttagccgtg acgggttgca gtaaaattga agaagactct
     7921 gcgaacttgc agaaagccgc tgctgaattg aaagcgcagg cggaggcgaa aaaggctgaa
     7981 gccgaaatga aagcacaagc tgatgcgcag caggcggagg cggatttaaa agcccaggcc
     8041 gaggcgatga aagccgaagc ggaattaaaa gccaaagaga tggcgaaaaa ggctacagtg
     8101 aaagtaaagc aggttaaaaa agacgtcgaa aaagtggcca acaaggtggc caacaaagtt
     8161 gccacggatg tcaaaagcgc cggccaagaa gtgaaggaag ctgcaaaaga gacggtggtt
     8221 gacgtcaaga gcgccggtac ggaagcgaag gaagccgtaa aagagaaagc ggttgaagtc
     8281 aaatccgccg taaacgatgc ggcaaccgat gtcaaaaatg ccggccagta agcgctctaa
     8341 gcgagatgat caaacagacg aatcggacat ttttaaagaa aatgtccgat ttttttgcgt
     8401 ccgcaggttc aggcgaggtc ctttttatcg aacttaagtt caaatctttt gccgctgacg
     8461 atttccggcg ccacctcggg gatgggggaa cccggaataa taaaacgagc ggtgctgttt
     8521 tgcagaatat ctttttcgat attaattcca ggcataattt ggacgagctc aagaccttcc
     8581 tccgtcagtt taaagacgcc aaccgttgtt acatacagta ctttccggcc tgctttaaga
     8641 gctttccggg cattgaaggt aatctgatcc actttgtcga caaatttcgg aacgcccggt
     8701 tttttaacac ggacaagtcc attctttaat ccgtaccggg cttcatccat ccagttcccc
     8761 acaaagatga tggtccgggc atattcgacg atatccgtga aaccgcccgg tcccacgaaa
     8821 tcaataattt tcggtccacg atgggagaca ttgacattcc cggctgaatc cacctgtaga
     8881 aaaccgagta cgcaggtatc aagtctttct tcatagatat gaaaaatttc cgcagtgctc
     8941 aggatgcgct gcgggctgat ggctgcgccg aagtacacgc ctgatgccgg aaggccgcca
     9001 taggcgccgc cttccgaggt gaaggtgatg tccctgtaca gaccggattc ataaagcatt
     9061 cgggcaactt cctctccgat gccgatcccg atgttcacga tggccccggg accggtcgtc
     9121 ttcacgaacg tgtcggcgca caggcgggcg aggatatcga cgaccttgcc gcgatacggt
     9181 gtaatatcga gaaaggtatt aattaaccgc atgagtttga cggcttcgtg atcgagttcg
     9241 gacgagccgg ctgtaaaatg cggcatgaac tgagtttgca gaattccgcc gacctgttca
     9301 ttgcgcgggt tgacaacgat tgcattgacg ttgtccgccg ggatggcgat ctgtgaattg
     9361 tcccggggaa taattttgga tacggagaca atcacttttc cgccgttgag gcgtgcggcg
     9421 gcggccgcat ctttgtaatc cagaatcaga gcagcatctt taaaatagat gttgccgtcc
     9481 ttgtcggcgt aaggcgccag aaaaagcgcc cggtcgatgg gtggaatacg atattccagt
     9541 tttccgtttt ttgccgtgat gaaattgttt ggagctccgg gagacaccgc ggaaccgcac
     9601 ccgacagcgg gatcgagaaa ggtgtccagc ccgaccgagg tctgaatgga ggcaagtccc
     9661 cgggcctgcg cctcgatcaa gaacgccagg gctccctgtg acatggtgtg cagttcgagt
     9721 tggccgtcat cggccagttg cagaagcgcc ttggcggttt ctccatgccc gacgatatag
     9781 cgggtgatga ttcccggcgc atcaagttcc tcgatcattc ccggtgtttt gccgcggccg
     9841 ccctgcgggc aggcgccaat cacggtcaga ttgcggggat gtccgctgcg atcaaatgcg
     9901 tcgcgcaaag cccagtaaaa gatggaggcg cgtcctgttg cggcaaaacc gcctatggtc
     9961 agcgttgctc cgtcagggat catggccgcc gcccggcggg cgctgacaaa cttcggcccc
    10021 agaccggcgg gtagataatc cagatcgcgt ctggtccagg tcaggcggaa atgcagaagg
    10081 cgggcgatgg ttgtaaacat tttggcaatg tttacgtcct tgagtgcgtt gatcgttcgt
    10141 cttacatcca ttaatgctcc aaaatgcctg ctctatggtt acggggatac aaaaactgtc
    10201 gtgacagaaa aacaggatca gcggatgatc tcataacagg ttttgacgtc catacccggt
    10261 tccagcaggg ggaaaagttt ctccatattt ttcatcaaat tggccgaatg cagtttcaac
    10321 gcatctttat cgctgtatct ttcgtaaaaa ataatagtct tgtcatcgcc gcggaccgta
    10381 tgcggaatat agtccaggca tccgggctcg ctcgccttca cctgcgggac gatctctttc
    10441 aggacttgaa tagcctcgtc catgttgccg tctttgactt tcaacgtcgc aatgagtgta
    10501 atcattcatt ttctcctttt tttatatttc acaatctgtg gcggcagtat atgagtaagg
    10561 ccggtgtgtg tcaaagaaat atttattgaa aaatatcaaa tgaatagata acctccccat
    10621 tctgtcttgc cggaaaacgg ggaggttgag ttgattatgc gtttttcaga aaagcaatga
    10681 tttcgttgat gtccggtaag tcggcgacag gatagactgt gacaaaacac accttgcctt
    10741 tttcatcgag aatgacattg gcgcgctgcg aaaaaccatt ttggtcgcga aacaatccca
    10801 gacttttggc cacgccgccg tggggccaga aatcagccag aagtttggta tgttttacct
    10861 tgatggcttt tgcccaggcc gctttggacg ggacgctgtc aacgctcaaa ccgacggcaa
    10921 tggtattgag cttatcgaat acttttttgt ttttttcgag cgactgcatc tgccgggtgc
    10981 agaccggcgt ccacgccagc ggatgaaaag ataaaagcac gcgtcgccct gtcattcggg
    11041 aaagcataaa atcttgtccg aattgatccg ccagggtaaa atttttaacg gacttgccca
    11101 gtataatgat tgttttttca gccatgagtc acctcccgga aaaagctgcc ccataaagcg
    11161 cagcggtttt tcagacaaag aacattctat cggatcagcc tgaacaataa ttgcagtatt
    11221 gatactctgt cacgatacgg cgggtaacgc aaggggatat ccaccagatt cgagcgaaac
    11281 agaatgccgc gccggtgcgt gaaggtatcg aaaccggcct tgccgtgata ggcgcccata
    11341 ccgctggctc cgataccgcc gaacggaagt tcctggcttc cgatatgcga aatggtatcg
    11401 ttgatgcagc cgcctccgga cgatgttttc tgcagaacct cttcctgaat ttctttgtcc
    11461 tcggaaaaac aataaagcgc cagcggctgc ggtctctgat tgataaaggc aatcgcctcg
    11521 tcgatatcgc catattcaat caagggaagc agcggtccga aaatttcctc ctgcatcaga
    11581 ggctcttccg gagaaacccg gtcaatgatg gtgggcgata tatacaaatc tttacgggca
    11641 tatcttccgc cgaacagaat ccgcccgctt ttcaaaagct cgagcagacg gttgaagtga
    11701 cgttcgttga taatccgggc gtaatccgga ctcctcatcg gatcgtcgcc gtaaaatgaa
    11761 aaaaccgtcc tgatcatttt ctcggtaaag tcggctttaa ttttccgatg aattaaaaca
    11821 taatccggag cgatacaggt ttgaccggca ttgaaaaatt tcccccaggc tattcgttta
    11881 attccgtact gaagagcggc atcttcgtga acaatgcacg ggctcttccc tcccaattcc
    11941 agggttaccg gagtgagatg gcgggcggcc gcggtcataa tgctccggcc gacggctgtg
    12001 cttcccgtaa aaaagatata atcgaatttc tcctccagaa gtttttgcgt ggcctgagcg
    12061 tctccctgaa aaaccgtgac atcccccgga tcaaacaact cgctgatcat tttttcaatc
    12121 gttgccgatg ttgccggtga aaattccgaa ggcttaatca gtgcacaatt gcccgcggca
    12181 atggcaccga ttaacggagc catgaccaac tgaaatggat aattccacgg accgataatc
    12241 aggacaaccc cgtaaggttt ctgatcaata cggcttcgcg acaagaaatg aatgaggggt
    12301 gtcgccgcac gccgggagcg tgaccatgac ggaaggtgac ggatgacatg atcgatttcc
    12361 gtataaagaa tgccgatttc gccggcatag gattcaaaaa gaggtttgcg cagatcgtcg
    12421 tacaacgagg ccatgatctc cggttcgtac cggcggatgc cttccttgag ctttttgagc
    12481 cgggcgatgc gagcatcaac gtcctgtgtt ttccccgcgt caaaaaatgc ccgcagtccg
    12541 gtcagcgttt gcgatatgga ttccggcgac atcaacatcc tccattcacc atatgtcctt
    12601 cctgtgataa aagtataagg agaagagcgc agagtgtcaa aggaatattt ccagacgaac
    12661 cattgttttc atggatcgca gcaatccctg ttgaatttca ctaaaaataa gcgtaaagaa
    12721 taaaaaaatc ttctctgaaa attgtctttt gaaaaaccat gactgtaaaa ccgttcaaca
    12781 aaaagcatgt ttgcccctgg tggttgtgtt ttacgtttga taatcctatg cgaagaattt
    12841 ttcatgaccc cgttacgatt ctcggttctt acgtccgccg gggcgatacg gcagtggata
    12901 tcggtccggg tatgggcttt tttacgattc ccctggccaa actcgtcggc cctgcgggta
    12961 aagttatcgc cgttgatatt caacccagaa tgctttccgc gcttgccgcg cgcgctcaaa
    13021 agcacggcgt tgccgcaatc atcagaacgc atctggccga gccggattct cttgcccttc
    13081 gtgaaaaagc cgattttatc ctggcattct ggatggcgca cgaggttccc gacctgaaaa
    13141 aatttttttc agaaatccgc aagctgatga aaccgcaggg atggtttttt ctggtcgagc
    13201 ctttggcgca tgtgtcaagg aaagattttt tacagacgat cgatctggca aaacagtcgg
    13261 gcttgctgat gaaggaatgt ccgaaaatcc gcatgagtca gagtgctctt tttacgcctg
    13321 ataaaataga aaggcacgaa cagcctcttt aaaattgatt tttggaaatc accggcttca
    13381 ggcacgttgc cttgcttccc tgccggcggg aattttgtcg acaatccatc tcggcggcat
    13441 gcatgccggc acattcggga gaatcaacat ccggcatcgc cgatgattca ggcggcgctg
    13501 atccatcatc tctatatccg aattcactag gcagccgtcg agctgccggc taggaaacgc
    13561 ctttacgtca cggtgcggct catggaagga aaactaattt acgtcattta caaaacggaa
    13621 gttgtttttg ccggcgtttt tgactttata catggcttcg tcagccagtt taatcagccg
    13681 gtccatttct tcactgtgtt cgggataaag cgcaatgccg atactggtgc cgaccacggc
    13741 ttgttcaccg ttgatcataa tcggctggga aacggtttga atcactttct cagcaatgtg
    13801 cgctgcgtta tcgggatgat gaatttccgt tgcaatgatt aaaaattcat cgcctccgac
    13861 gcgggcgact gtatcggttt cgcgcacgca ggaaagcaga cgctgagcaa cctgcttgag
    13921 cacgtaatct ccggcatcat ggccgagcgt gtcattgacg ttcttaaaac catccaggtc
    13981 gaggaacatg atcgccgctg actttttgta ccggcgcgcc atgttcattg ccgagaccat
    14041 gcgatctctg gccaatcgta aactgggcag atccgtcaat agatcgtgcg ttgccagatg
    14101 tttgatctgc tcttcggatt ttttgcgttc ggtgatatcc cgggtgacac ctaaaacacc
    14161 gatgggcttc atggacgcat cgcgaataaa cgtcagagaa ctctccagcc agacccaatg
    14221 tccgttctta tggcggagat gggatggaag tgttcgactg cggttcggat cggcggtcgg
    14281 atcgttttct cgttcgagtt cctctgcgag caatttctga gcgtcaacca gcgattcggg
    14341 cgtgaaaaat tcgtcgatct ttcgcgccat gaattcgtca gccgtatagc ccagtgtttt
    14401 gattaccgag gggctggagt aagtaaagcg catgaacatg tccatcgtcc agatgtgatc
    14461 ggcggtgtga tcggcaataa aatgataatt ttcctcgctt ttacgcagag cctcttccgc
    14521 ctgcttgcgt ttggtaatgt ccagaataaa gccgcgaata ccgaccggta cgccgtccac
    14581 cgcgatcggt accgataacg ccagagcatg aaaaggactg ccgtcttttt tcagagcttc
    14641 atattccagg tatccctttt gggtgccctg tattctgttc tgtattttct cccaagctgc
    14701 tttacgatca ttcggcgata caaaatcaaa aatgtttaca ccttgggcga tgtcctgttc
    14761 ggtaagaccg aaatgtttga ggccgtgttc attggcgtac gtcagattgc cttttgcgtc
    14821 cgcctcgaaa atcgtttcgg gaaacacttc ggcaagttgc ttgaaccttt cttcgctttt
    14881 gcgcaatttc gcctcgccca ttttaaggtc caccgatttc cagacggcat ccataaacag
    14941 ggtgagttgc agcacatcgg tttcgtcgta gtcgcgggcc ttgtttgcga tgccgaccac
    15001 agcgacgatt tgatcctgat cgaaaaccgg cacggttaaa aacttgaaaa ggggcgcatg
    15061 gccttcagga tatcctttct tgagaggatg atcggccgga aagtcattca caatgatcgg
    15121 tttgcgctgg cgtaccgctt ctccccagat tcccgttttg gccagttcat aacgcgtctg
    15181 cggattcggg atggtgcatt ctttcatgac gtcctttgac caggagttca ggacaaattg
    15241 ccggctgttt tcattgtata aataaatata accgaccttg ctctccgtga gtctgatgac
    15301 ttcatcgagc gcgtaatcaa gaaactcctg tgttgtctga gtacgatgct gcatgatatt
    15361 taagaggccg gtcaagcgcg ttgcgttgcg ttggatttgt tcttcggcgc gtttttgctc
    15421 gatgaaatgg ccaagctgtt cggctacggc tttaagcaat tgccgctcgg aatctaaaaa
    15481 aggtttttca tccgtctgaa accgttctgc cggataacag acattaacct gaccttccgg
    15541 tttgccgttt acggtaattt tttcagtgag ctgccaaggc gtttctttga aaaggcctgt
    15601 ttgaaaaacg gtttcttcca gaataatcct ggcccctgtt atatcgggga accgcatagc
    15661 cgcaggcaga agcttgaccg ccttattcat gatgtcgttt aatgatatgc caggctcatc
    15721 cagcacagcc gaaataccat agagacagtt taattcttta attcgttctt taagcgtcca
    15781 ctccgcctgc ttttgctcgg tgatatcgtt gatgctggat aaaagacagg gttcctcggt
    15841 aatgttgatg atctgcgacg aatacaaacc ggtcaatatc tccccgttct tcttgcggaa
    15901 cctgtactcc tttcgtacga ccggcaatcc ttgccggaga atggaaacca tttcttcacg
    15961 atcttcttta tccacccaga gattcaatcc catggatgat tccttcatga tttcttcacg
    16021 cgagtaacct gtaatattaa caaacgcgtc gtttatatcc aggaatttgc cgtctttcat
    16081 ttgcgtaatg gagatggcat aaggcgatgt ctgaaacgct ttggcgaatt tttcctgatt
    16141 ttgacgcaat tcctctgcgg tccgcatccg ccgggtaagt tcgttctcaa gttctatggc
    16201 aagactccgg ctgatcagca gaaaaagaga aaatgtcaag gcgaaaaata atgtttcgta
    16261 aatcaggacc gcaatgacgc ccgcagcgcc ggatacaaag gcgttgtcgg acggtgtttt
    16321 aatcaaattt atgatgattt ggatgatatt gacaatgcaa aacgccgcaa aaacaattcc
    16381 cgttattttt gtggcctgac gcaacgggga ggccaccctg taaagcatca gccaggcgcc
    16441 ctggatacag atataaaaaa gcgcgactga aaaatttatc gtgcgcaaac ccatatgagg
    16501 ggcaatatat gtgaaaaaaa tgtgaaccga ggtgaaaatg gccagcatga cataattgtg
    16561 tttctggcga ctttcctgcc ccacgtaacg gcaaagacct tcgtaaagga tcaccgttcc
    16621 gccgacaata aagagattgg cgacgacgat tgtcataaag tccggcagga tgcctcgcaa
    16681 tgcgatgagc ataatgcccg caaattgaag aatataatcg ataagccaaa gaatgatttc
    16741 cggagcacgt ttgcggtttt gccaccagag agaaacaata acgatcaggc aaagcaatac
    16801 agacaagcca tattcggcaa agatgatcct catatcaatt tcaatcatgg taacattcct
    16861 ttgatggcaa catcacgtct gttcttgaaa aatattttat gcgtcgtcat gttttaaggt
    16921 ggtggaaaat aaggggtcct atccttcaat tttcccggat aattcgttgg taaagaatca
    16981 agaagtgaca aaattacata acatgtgtga aatctaatga ttacagcatt gcctgattgc
    17041 aaatgcaaag gatgaaaaac aattgctttt gcggtttttc aaacattgct attgacttat
    17101 attatgaatt ctaaatcaat atcttgtgaa aagcaattgt ttcttcacca aagcggtgtg
    17161 ccgttaaaat cgggaaattt tcattagcga ccgcgggaac cgcccagaat tgatcccagc
    17221 actccccgga taatctggcg tcccaaagaa gaaccgatgc tgcgcgccac gcttttggcc
    17281 gcggcctgca ccatgccttc tctctgtcct ccgcgggggc ctttgctgcc gaaaatcatt
    17341 ttgctgagag catcccccat gcctccgctg tccttttctt cttcactttt tgccggagca
    17401 gcggaacgct ccactttaat ggcgggtttt tcttcaggcg tttcctcttc ctgttgtgct
    17461 tcccgcagtt tttcataggc ggattcgcga tcgacggttt tttcataatg cccgtaaatc
    17521 gtggaagcct tgatagcggc taggcgctca tcgggcgtca tggcgcccat gcgtgagaaa
    17581 ggaacaatta cattgcatcg ttcgacgatg ttcggtcgcc ctttttcatc aaggaaggaa
    17641 atcagcgctt ctccgacacc cagttcggta atggcctgtt cggcatcaaa ggccggattc
    17701 tggcgcatcg tggaagccgc tgtctgaact gctttctgat cacgcggcgt gaaggctctc
    17761 agagcgtgct gtacacggtt gcccaattgt gccagaaccg tatccggaat atccagcgga
    17821 ttctgggtga caaaataaac gcccacaccc ttggaacgaa tcagacggac aacctgctcg
    17881 attttttcca gaagcgcctt gggcgcgtcg ctgaacaaaa gatgtgcttc atcaaaaaag
    17941 aagaccagct tgggcttttc gggatcgccg atttccggca tctgctcaaa caactcagcc
    18001 agcatccaga gcagaaacgt cgaataaagt ttgggattat tgtagagctg atcagcggcc
    18061 agaatgttca tgaccccgcg tccctcgctg tccgtctgga tcagatccga aatgtccagc
    18121 atcggttcgc cgaagaattt gtcgccgccc tgctcttcaa tggtgagcag tccgcgttgt
    18181 atcgcgccga tggaggcggc ggagacattt ccgtattccg tcttgaattt tttggcattt
    18241 tctcccacaa actgaaccat ggcccgcaga tctttcaaat cgatcagcag caatccgttg
    18301 tcgtcggcga ttttgaagac aatctgcaaa acgccggatt gcgtgtcgtt gagattcagc
    18361 agacgcgcca gaagcaatgg tcccatatcg gatatggttg cgcgcaccgg atgaccggat
    18421 tttccgtata catcccagaa aaccgtcgtg ttggcccgag gctgataatc tttcacgccg
    18481 atgccttcca gacgtttgag cagtttttcc gaggcaacgc cggccgcgcc gatgccggaa
    18541 agatcgccct tgacgtcggc catgaaaact ggaacaccga tggacgcaaa ggattcagcc
    18601 attttctgca acgttacagt cttaccggtt ccggttgctc cggttatcaa accgtggcgg
    18661 ttggccattt gcggcagtaa tgtaatttcc ttcccctgcg acatggcaat aatgagcggt
    18721 gcggtcatgg ttttctcctt ggggcatatt tatttaagac ttcgaaagta tagggataat
    18781 gatatagcat gtcaatgatg aaatcgtgat tgtccaggcg ggaatttatt tcaattattc
    18841 tgatggatgt ttcataaaga cgttaaactg ttttacagaa ttgttacatt ttgtataaaa
    18901 attctgatca gcgaaaggtt gtcggcttta aactgttaca attgtgttct gtctttaggc
    18961 gaagagcaca aagatgtaga aatttcctcc cgtgccaaat tcataacctt acgtatttta
    19021 tagggaatgt gtgatggcag gattattgct aatcattaat tattttcatc gggaggaaca
    19081 ttatggatct acagataagc gcgggcgata caggatggat actggtttgc tgttctcttg
    19141 tgctcttgat gacccccgcc ctggcgtttt tttacggcgg catggtcaga agaaaaaatg
    19201 ttctatccac cttaacgtta agttatatct ttatgtcgct gatcggcgtg cagtgggtgc
    19261 ttttcggata ttctctttct ttcggcccgg ataccggcgg actgatcggc ggattggatt
    19321 acatcggctt tcagggcgtc ggcgccgaac cgaacgccgc ttattctaga accattcctc
    19381 atgagctttt cgccgctttc cagatgatgt ttgccgtcat tacgccggcg ctgattacag
    19441 gggcttttgt ggagcgagta aaattcaaga gcttcctgat gttcagcctg ctctgggcaa
    19501 cattggttta caatcctctt tgtcactggg tctgggggca gggcggatgg ctgaaacaga
    19561 tgggcgttct ggattttgcc ggaggaacag tcgttcatat tgccgccggt ttttccgccc
    19621 tggcgtttgc attggtcatc ggtccgcgca aaggtcacgg aagcgttccg atggaaccga
    19681 acaatattcc gcttacggtt ttaggaaccg gtttgctttg ggtcggctgg ttcgggttta
    19741 atgcgggcag cgctctcgcg gccaacggcg tggcggtgaa cgctctggtg acgaccaata
    19801 cgtcggcggc ggccgcgggg ctggtctgga tgctgctttc ctggctggac ggaagaccga
    19861 gcacgctggg catcgccacc ggcatggttg taggactcgc agccgtgacg ccggccagcg
    19921 gttttgtaac gcctctggcc gcgatggtga tcggagccgt gtctgctcct cttgcttatt
    19981 atgccatccg tttccgggaa cggcgcaaac tggacgagtc tctggatgtc tgggcctgtc
    20041 acggcatggg cagcacctgg ggcaccgtcg ctacaggatt atttgctacc gtggccgtta
    20101 atgccgacgg cgccaacggc atgtttttcg ggaatgccgg ccaactcgcc attcagatta
    20161 ccgccgtttt ggtcaccata ctcttttcgt tcggcatgac ctatattctg gccaaggttc
    20221 tgcacgtgag catcggattg cgcgtcagtc cgatggaaga agaagtggga ctggatatca
    20281 gttcacacgg agaacggtcc tactaacaga acatgcttga atggaaacat tgatcagttt
    20341 tttattttta aaccggagag gttaccatgc tgaaaaaaat cgaagccatt attcgcgaag
    20401 ataaagtgaa cgatgtcatg tgcgccctga aagagattgg cattgtgggt atgagtatgt
    20461 ttgaagtgag gggacacggc cgccagggcg gcgtccagct ggtcggacgc gccggtacct
    20521 atcaggtcaa tctgcttccc aagatacaac tcaatatcgt tttaagcgaa cgcaacctgg
    20581 aagcggctct ggagacgatt cttaaagcgg cctacaccgg cgagacgggc gacggaatca
    20641 tttttgtcac accggtagag gatgtcattc gcgtccgcac gcgtgagcgc ggagagggtg
    20701 cgatgatata tccgggtgat attgacgaaa gaagaaaaaa ataaacagag agatttgatt
    20761 ttctgctgat taggaaacct gagaaaaaaa taaagtgccg attcattgaa gtatcggtgc
    20821 agataaaaca tccttatttt ttcattatcc ttttcatcga taacaaaaaa atccggacca
    20881 cccgatcccg gaagactaat gaatggttta gccacaaatt catgcctggt ttaatataga
    20941 aagcttttct cgcaatcccg cgaaagcaac catttatgtt tgtggtcatg aatccttcag
    21001 ttttagctga aaccgaaaat catcatcacc tgctgcgatg ctactgactt tgaccgcggt
    21061 tgttttttca ccgaatattt tgaacgcctg ctggttgaat cccataacga gctcctcgaa
    21121 atagttattc ttcactggaa ttccggtgat agtgatatga gcggtgttgt tatcgaaatt
    21181 tgacgtaaac gtaccgccat caaaataagt tgaccagatt tttggcatgt tcagttcgac
    21241 aaactgcctg gcatccttgg caagcatata agatttgtag ggacctgtcg gagagagaac
    21301 aaatttcgcg cccgattttc caaacatggc gtattgagtt ttatcattgt tgaaaaattc
    21361 cctgcatagt tcattaaaaa aaacaatcat cttctctacc ggaataaggg taacggacat
    21421 aatcatattg ttgaaaaaac catctttttg ggtaagaccg gcgtgaaatg cattccatcg
    21481 ctcttcgccg aacgcttctg ttatgatagc cttggtcgtg acaaaggaac ttcccttaat
    21541 attcatatta agcgccctcc ataaattttt ttgtaaattt aatactcaag gtatgaggtt
    21601 gcaagtaaaa atattaattc gttgatcagg tacgaaatgt ttttgcgtat attgaatgca
    21661 tgggaggtgc gggtataatc ttcatgaatt aagtttttag aaagataaat cgaagaggcc
    21721 gacgatattg ccgtttcata aaacaatcat tttctcttga cacgacatat ctgttacttt
    21781 aattactagt ataatgatag tatcagtaag tatgatgtaa aaggacagcg taatgaaaat
    21841 ttcaacacga acgcgctacg gcgtccgact gatgacggcc ctggcccgca attacggcaa
    21901 agaccccgtt ttcctcaaaa atattgccaa agaagaaaac atatccgaaa aatacctgag
    21961 tctgattatt atccctttgc gagggggtgg tctggtcaat tcggtccgag gagcttacgg
    22021 cggatataac ctgaacaaag atccctcgga gataacttta aaggaggttg tcgatgtttt
    22081 ggaaggagac cgctttctgg ttgattgcgt taataatccg gccgcctgtc cccgcgtgac
    22141 gacatgtgcc agccatgatg tctgggcggt tgttggagaa aaaatttcag aaaccttgag
    22201 ctccataacc cttgatcggc ttgcgaagat gaatcagaaa aaagaaaaat aattatgcag
    22261 aatatttgaa agaaaagata actgctgcaa aacatgaatt tgatatttga tcctgaggat
    22321 aagaatgggt gataaagttt tggtggccat gagcggcggg attgattcat cggtggccgc
    22381 atggatgctg cttaatgaag gttacaatgt tgccggcgtg accatgcatt ttggcgcctt
    22441 gaccgaggga gacgggaaaa gatcctgcac gtccgccgcg attgacgacg caaaaaaagt
    22501 ttgcgcctgt cttaacattc cccattatgt ttttgattac gcctcccggc tgggaaatga
    22561 ggtcattgcc gattttatca gcgaatatca gaaagggaga acgcctaatc cctgtgttcg
    22621 ctgcaaccgc catctgaaat tcggttcact gctggacaag gcgcaggagc tgggtttcaa
    22681 ttatctggcg accggccatt acgcggccat tgaaaaaaac gaaggcggtt attgtcttac
    22741 aaggccgaaa gataaaaaaa aagatcagac gtattttctc tatgccattc cctatgagag
    22801 attaaaaaat atattattcc cccttgctca actcacgaag gatgaagtga gaaatttggc
    22861 gatgaaaaat tctctgcccg tgacggcaaa gcaagagagt caggatattt gttttgtgac
    22921 tcagaaaaat tctcagacat ttcttttgga aagagtaaaa gacatcgcgc cgggtccgat
    22981 tgttgatgtg cggggcaatg tgctgggcag gcatcgcggg attatctttc atactatcgg
    23041 ccagcggggc ggtctgggta tcagccatcc cacgccgttg tatgtattgt ccattcatcc
    23101 cgggaacaac cgcatcgttg tcggtgacaa gaaagacttg atgggcaaag cgcttatcgc
    23161 gtccggcatc aataggctgg cgaaatcctg gccgcagcag atgcatgcga agatcagata
    23221 ccggaaaaaa gaagcgcttt gcgaagtccg ggaagaaaaa gaaaaattga aagttatctt
    23281 tgccgaagaa caggaggcaa ttacaccggg tcaatcggta gtcttttacg atcacgattg
    23341 cgttctgggc ggcgggatca ttgagaaagt catccctttc ggataaggca aaatacaggt
    23401 aaaagaaaca aaaatgaaaa ttctggcggt gaacatctct cagaaaaaag ggggaaaaaa
    23461 gaataatatc ggctgcggtc tttttttaga aaatctgggg ctggaaagcg atgcccatgc
    23521 ccaggccgat atcatccggc aggtcagtct tctggccaaa gaaagcattg aaaaaatcag
    23581 agccaaaggc cttgacgttc aatacggcga ttttgccgaa aatctgacga ccgaaggaat
    23641 tgatctgccg tcgcttcctg tcggtacaag gttgcaagcc ggcgccaatg ttctgctgga
    23701 ggtttcgcaa atcggcaagg tctgccataa tcgatgcaat attttttata ctgtgagcga
    23761 ttgcgtcatg ccccgggaag gtatattcgc taaagtgctc atcggcggag aaattaaaat
    23821 caatgatgaa atccaaatca gtaaataaaa tatcgctgaa tcggtttata tgatcaaatt
    23881 tgcatttgta ctcaatccct tcaatattca aaatgtttac gattattgtt tcctttcaag
    23941 acttgcacct caggcgttga taaaaataat tgtaaggttg ttgccgcctt ttaaaattca
    24001 tcgtgcaaaa aatttgcaat ctgccgctgg tgcggaaatt gatggcagct tcattgtctg
    24061 tccgctgctt tctgaacaga ttcttgctat gaaggaaaat gccgttttaa ataagatcct
    24121 gaaagcggtg cggatcgggg aacgaagcgg agcaaaggtt gtcgggctgg gagccttgat
    24181 gggtgttgcc ggaaaaggag ggcagaggat tgcggagcaa acgcccgcgg cgatcacgac
    24241 gggttcgagc ctggcctcgg cagccattct ggagacgctg gacagagcgg cggcgatgcg
    24301 taatgtggat ctctccaagg caaaagtagc catcgtcggc gcaaccaacg ccatcggcca
    24361 gaattgcgct ctgggatttc tcaacagggt ggatacggtt tttctgctgg ccaggaacgc
    24421 cgaccggctc gcggagctga aggaatttct caacgctcaa aaatcaaaaa caaatattat
    24481 tgatcaaggt cctgcgttgg aggatgtcat cacggaagcc gatcttgtca tcttcaccac
    24541 gtccgccatc gaggtgcctt ccgcagcaac agctaagaat cttaaaaaag gagcgattat
    24601 ctgtgacatt ccctccccgc gtaacattgc ccgggaaatc tgcgatcagc gtaaagacat
    24661 ccttgttatt gacggcgcag tgatcgaacc cccgccgacc gcgcagctcg gattaaaact
    24721 gccgatccaa gacggatata tttacgcctg catggcggaa accatgatcc tcgcttttga
    24781 aggacagacg caggatgatt tttccacagg cttcagaccg gatttgcata aggttgcccg
    24841 gatcaaagcg cttgctgcaa agcacggctt caacatcaaa ttcaccagct tcggcgtgcc
    24901 ggtttaaaat attgataaaa gtttattttc acgttcatat gcgatatttc attgacatac
    24961 catattgcaa tacctataat ataaataagt aaaaatcatt tattatcaag caagcataag
    25021 ggcatgctcc atcacgggag ttttgtcttc aaatgttttc aacctgtacg taaaaacttt
    25081 taacaaccaa aatatcaaga catgggtggc cgatatggat gatgtaatca cccggattgt
    25141 cgatattgaa aagcaatgct ctgctgatgt agaagcaaca cagcttgagt acgggaaaaa
    25201 cattgaagcc cacaaacggt ttcttgaagc aaagaagacc cgagaacgcg ccgagatttt
    25261 atccgcagaa aacaaaagac agtctcaagc cgtagaagac gcaaaacgaa acatagaggc
    25321 cgtctctgca gattttatta cggatatgga aaatcttttc cgagatcagg ggctgaacga
    25381 ggcgataaaa aaagacatag tttccatttt acttggaggc tagtggtcat gagaaattac
    25441 gccgatcagg ctaatcttca cgcccgtata tatgccatga aagggcggct cctctctctg
    25501 caggactatg ccgtcatggc cagggaacaa cggacgtttg caccaaagtc atcaagcgtt
    25561 caggatctga ttgatgcaaa agagaatctt tttagggaac agatagcacc tgttatcgca
    25621 ctggttgaag catacgacaa atacactcca gtatttcttg cttatctccg tcaattcgag
    25681 acacacaacg ccaaggttct tctggcaaag gcagcaggaa aggacattct ggaacaatgg
    25741 tatgatatca gtccttttgc ttccctggat aaaagtctgt tgaacaagaa tctatccatg
    25801 gatgatgtca cgtcacttct taccaatact tatcttggag ccgatttcaa gccctcttcc
    25861 agttaccggc gaatgcagat gaatttagat atcagttccg cgagaaatat ctataacgct
    25921 tcatctttaa tttccgacca ggccagaaaa gaatttcagg aaatgatcct gagaagaatt
    25981 gcggtacaaa tggtgatccg gtcatacagg cttcgcgttt actatcactg gagcgagaag
    26041 gacacccggc tttatttgga aaaaataaac aatcgcttaa acatccatgc ggaatctcaa
    26101 atccattcaa tagacgagga tttaaatcag catattgaaa cgttgcgcaa aagcagcggg
    26161 caggaaccat ctgtcattga tatcgaacag catcttgagc agatttatta cctatggctt
    26221 tcatcaattt tttatcgaga ctttcactcg atttacggtg ttattgctta tctagggtta
    26281 ctcctcattc agataaaaaa cctgtttcgt attatcgacg gccggcgttt cggtttttca
    26341 gccgatgcga tactcagtaa aataatatgt aaagaataat taaaaaaatg tttttaaaat
    26401 ctgacatacg tcacattacg atcgctttgg aaaaagccta ttattcggac gtataccttg
    26461 cattgggtca ggcaggtatc attcatctgg cgcgtttttc agagaaggat tccggaacgg
    26521 acagcggact tcagaaagaa gaggcattaa ccagagaaat tatttcgaac acagaatacg
    26581 cgttgaatgc actgatgatt gccccggaag aatccgagat atttatgccg gctgtcgata
    26641 agagcggcga tttgattttt gtttcccgga taaaaaaaac cattgaacaa gccgtgcgac
    26701 tcagggcaaa gatcagggaa aaggaaaaca ttatcattcg acagatgggg tacgctgaag
    26761 cattgagtaa gatggctatc gatcctgtcg cgataaaaaa ggcgaggctt gttaaaatcc
    26821 ttttcggcac ggtggaaaat atgacggatg atgttccacc gaacagtcgt ttcatgttaa
    26881 acaaaattaa caattacgtt ctcggtattt gcttgcctga tgattatccg gctctctttc
    26941 gttttttaaa aaactatgga tttatagatt tatcagatga tgtaactcct gtttctttgg
    27001 aaagtttaaa aaagcggcgg gaaggtctga aacggcgccg cgacatgatg gatgcatatc
    27061 ttgatcgact gaagacagaa caaggccgct ggcttcagaa tctgaataaa tcctaccatc
    27121 actacgagga aatactcaag gcgatgcgga tgtcgctatt ttcagcgaag gcattgttta
    27181 ttacgggttg gatagacatc cgaaacaagc agcggctttt gaaaattctg gaaaagattt
    27241 gcggcaatag atttattttc tctgagcgta aagatccgga tgcccctgtt catctgcgga
    27301 acatgaaact ttttaagccg ttcgagctga tcgtgaaaac aatgggtatt ccgtctaata
    27361 gagaaattga tccgacaccg cttaccgcaa tcacttttgt gctcatgttt ggtctgatgt
    27421 ttggtgatct gggtcaggga ttggtgctcc tgctttgcgg gataatgctg aaaaagtatg
    27481 gccaaaaaaa aataaaggaa aatctgaaac aagccggcgg cattctggtc gcttgtggat
    27541 tttccgccgc catttgtggt ttaatttatg gcagcgtttt ttccagcgaa cacctgcttc
    27601 cagccctttg gtttcacccg actgcaaaca ttatgacact gtttgccgca acgattctca
    27661 taggcatttt gtttatcatg accggtcttc ttgtgaacat catcaataat atcctgaacg
    27721 cggattacac ggaagccctt ctggataagc ggggactggt gattctggta atttatgccg
    27781 ctactgtcct ttttgcgttc aattatcaaa ggtccggtca ggttcctgca atatgggaga
    27841 tcagcgcttt tataatgctg cccctgacat tgtttgcact cagaggcgtc attggcccgg
    27901 cattttttca tcaagagagc ccgcacagtg cttcagaata tatcatagaa accgtcatgg
    27961 aaattgtgga aaccgggctg agtctgtttg ccaacacgat atcatttatc agagtcggtg
    28021 cttttgctct ttcacatgcg gggttgagta tcgtgaccta cacgcttgcc ggaattgccg
    28081 accccggttt gcaatcagcg ggtgcgatct taatcatcat taccggtaat atttttatca
    28141 tcggttttga aggtttaatt tgcggcattc aatccatgcg tcttgaatat tatgaattct
    28201 tcagcaaatt tttccaggga gatggagtcg tcttttctcc atttacctta aaggcacaag
    28261 catcggaggt gtaagatgat caagaataaa ttgttcaagc attttgtgtt tgcagtcttc
    28321 accggtttca tttggttcgc attgttgccc accgtcatta atgcagccga gaatgttcag
    28381 gttaactccg gagcaaactg gatgggtttg gcttacatcg gtgcggggct ggctgtgggg
    28441 ttggcctgta tcgccagcgg ctttgccgta gcacggatag ggtctgccgc cattggcgca
    28501 gtgagtgaaa agccggaact catgggaaga acgctcgttt ttctcgggct ggcggaaggc
    28561 attgcgattt acggtttgat tattgcgatc atgattttaa ataaactgta attacatttt
    28621 caaatcagga tgataccgcg gcggcagtga aacttaaatt acggaaacga agtgaaccga
    28681 aattaaatgg ttgaactgtc ccgcttcagc ggggcaagga ggaggctccg caggcgtatt
    28741 gctaacccag tgacctttag gttatacgtc gaggaagccg acgacgccaa caaaggtagc
    28801 gcttgacttg gaaagggaat gtcatggaaa agatatacat catcggagac atgcatacgg
    28861 tcagcgcgtt tcgccttgcc ggggtggaag ggatggtctc aacggcagac agtgcatcgg
    28921 tgcgactcga agaaattata aaaaaaagag atgccggtat cgtcttgatg acgaatgacc
    28981 ttgctgcagc attacaggag cgtatcagag agattaacct caataggcag aaccctgtgg
    29041 tgatagaaat tccgggcatg gatgatatcg gggggctgcg tatgtcggca atgaattata
    29101 tttccgaggc tctcggtatt cgtttgtaag gatagagcat ggacaataca acattggaaa
    29161 attctatccg tgaagaagcc tctcgcgcaa taaccgccat cagggaaaaa gaggctctgg
    29221 agatgaggca gagggatgag gtcttttcga tggagatgga ggacttccgg aggcaggctg
    29281 aagcggaaac agaaagtcac atccggcagg aaatttctaa actggaaaat aaagcgcttc
    29341 tcgagcgaaa aaagatgaag cttcagaatg cagagcaatt tatcaatcgc attgtcgatg
    29401 aggtcatgaa aaacattcgt aacgatcgcc tttacaaaca atttcttctg gaagcaatcc
    29461 gcgatggcgt aaaaatatcg ggtgctgatg tagatataaa actcagcaac gaggatattt
    29521 tcctgaagaa agatatcgtc gcagccatcg cggctatgga cggaagaaag aaagtttcca
    29581 tcacggaaga ttcgaaagtc caatggggcg gatgtctcat ccgggataag gaaacaggtc
    29641 gtatttttaa tcatacgctg gaaagaatct atttcagaaa atctctgctg atcaggcagc
    29701 aagtcatgag tttactgatg cggaaagcgg aaggaaatca atgacgcagt taacttttaa
    29761 ggagcgtatg tggacgaaat attaaaagga aatgtgattt cggtgaatgg gcctattgtc
    29821 aaggttgtcg ggctgaccgg aataaaaatg ttcgacattg ccgaagtggg acaggaacga
    29881 ctgatcggag aagtcatcag gcttgccgga gaaatcacga tagtccaggt gtatgaggat
    29941 aacacaggct tgaagccggg tgacgacgtc atttcatacg gacgtcctct ttctgtgctc
    30001 ctggggccgg gactgatagc caatatttac gatggtattc aacggcctct cgtcggtatt
    30061 tccgaggtat gcggaagcta tattaaaaaa ggcattaaac ttccgcccct cgatgtcaat
    30121 aaaaaatggg acttccagcc acgggtcaaa accggtgagg atataaaaga ggggacgatg
    30181 cttggagaga tcacagaaag cccgctagtt gtacatcggg tgcttgttcc gccgggcatt
    30241 gcagggagat tgaccgctat cgtgaatgcc gggtcttaca cgatagaaga cgatatcttt
    30301 gaaattgata caggttccgg caagtacaac gggaaacttg ccgcttattg gccggtgcgc
    30361 aagccccgtc ccagccgaaa cagaaaaaaa ccttttatgc cgttaattac aggccagaga
    30421 attatcgata cgtttttccc cattgccaaa ggaggaacgg cagccattcc cggcggcttt
    30481 ggcacgggaa aaaccatgac acagcatgct ctggcaaaat ggtgtaacgc cgatattatc
    30541 gtttatatcg gctgtggcga acgcggcaat gagatgacag atgttctcac agacttcccg
    30601 aaacttattg atgaacgcaa cggccggcct ctgatcgaac gaaccgtgat gattgccaac
    30661 acatccaata tgccggttcc cgcccgcgaa gtaagcatct ataccggcgt gacgattgcg
    30721 gagtactacc gcgatatggg ctacagcgtg gcagtcatgg ccgattccac atcacgctgg
    30781 gcggaagcgc tgcgggaact ttccagcagg ttgggcgata tgccggccga agaaggattc
    30841 ccgccttatc tggggacacg gttggccgaa ttctacgaaa gggccggtct tgtggaaacg
    30901 ctgagcggca gtaacggcga cattaccatc atcggagcgg tttctcctcc gggcggagat
    30961 ttttcggaac cggtcacgca acacacatca agatttgtga gatgcttctg ggcgcttgat
    31021 aaaattttag ccgacgccag gcattacccg tcgattagct ggatcgacag ttatacggaa
    31081 taccttgtcg atattgaaga ttggtggaaa aatctcgaca gcgaatggct gacaaccaga
    31141 aatgaggtca tgaatattct tttggaagac catcgtttgg agcaggtcgt gaagcttgtc
    31201 ggcagtgacg cattgccgcc ttctgaacag tatattctct tctgcgccga aatgatcaag
    31261 aatgccttcc tgcagcagaa ttctttcgat tcgcaggaca agttctgcag tccggaaaag
    31321 caactcaaac ttttaaagtg ccttttggtc ctctaccaaa aaggcatgac gctgattcaa
    31381 gaaggtgtgc ccgtcaaaga tatcagtcaa tcggacgttg tttcggaaat ggtacggctt
    31441 aagtcggcag ttcccaataa agaacttgtt gagattgacg attacttgaa aagactggaa
    31501 caggaattga tgtcgataaa atcgtcatcg tgaggattgt aaaatgacca tggaaattga
    31561 atatacaaac ctttccggaa tttccgggcc cctgattttc gtcgacggaa tcaaaggcgt
    31621 cggatacggc gacattgtag aaatccgcga tgcttcagga gatttacgta tcggtcagac
    31681 gctggaggtg gatgaacaga gagccgttgt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnggagattt acgtatcggt cagacgctgg aggtggatga acagagagcc
    31861 gttgttcagg tcttcgaggg gacaagcggc ctttcggtga aagaaacaag agtcaccttt
    31921 gtgggaaggg cgttggaaat acctgttgat cgtgaaatgc tgggcagagt ttttgacggc
    31981 ctgggcagac ctgttgatgg cgctccgcag gtcatcacag acaggatgat gaatgtcaac
    32041 ggtctgccga ttaatccctg gagtagagaa tatcccagag atttcataca aaccgggata
    32101 tcggcgattg acggcatgaa cacgctgatc cgcggccaga aactcccgat tttctcggga
    32161 agcggtatgc cgcacaatcg tattgccgcc cagatagcca ggcaggcaag aattctgact
    32221 gcagaagagg agttcaccgt tatctttgct gcgatgggag tcaaatttga cgtagccaga
    32281 ttcttcatcg attccctcga agagtcagga gttctcacgc attccgcaat attcctgagt
    32341 cttgccgact caccatccat cgaacggctc gtaacgcctc gttcagcgct aacgcttgct
    32401 gagtatttgg ccttcgaaga agggatgcat gtactggtga ttttaacgga tatgacaaac
    32461 tactgcgaag ccttgaggga actctccagt tcgcgtggtg agattccgtc acgaaaaggt
    32521 tatcccggct atctttacag cgatttggct tcagtctatg aacgtgccgg tcgactcaaa
    32581 ggcggtaaag gctccattac ccagatgccg attctgacca tgcccagcga cgacatttcc
    32641 catcctgttc cggatctgac agggtatatc accgaaggtc agattgtgct ggaaagagag
    32701 ctgtttaata aaggaatcta tccgccgatt gccggcctcc cttccctttc ccggctgatg
    32761 aaagacggta tcggtgaagg tcgaacacgc aaggaccatg gccaggttgc cgcgcagctc
    32821 tttgcctcgt atgctaaagt caaacgcata agagcgttgg cggccatcgt gggagaggag
    32881 gaactttccg atctggacaa aatttacttg aaattcggaa ccgcttttga ggaggctttt
    32941 ttgcgtcagg cttatcatga gaatcgttcc attgaacaga ccctggaaat tggttggaag
    33001 atgctttctc tgcttccccg tgatgaattg tatcgcattg ctagagagga tatccagcga
    33061 tactatatgt ctgagacgga gaaaacgtaa tgccgaaaat agaattaccc ccgacaaaat
    33121 catcgctgcg aaaactcaag gaagatctgg ctttcgctta cgaaggctat gatctgctta
    33181 atcagaaacg ggaaattctg gctattgaaa tcgttcggaa gattgatgtc atccggaaaa
    33241 ttgaagccga atttcaggat attgtaaatc gtttatattt ggcttatcga caggctgccg
    33301 tcgatatggg ttccgaagct gttacattac aaagttgttc cgaaatgaga agctattatc
    33361 ttcatatgaa tttaacaaaa ctgatgggac tgaaactgtt aaatttcact cttgatctca
    33421 agaacaatct tcccgctttt actttgccgc caacaacatc tgcttatgat aacgcaaaag
    33481 aacaatctcg gaaagctctt cacatcgtgg tgcaatacgc gacaataatg aaatccgtca
    33541 tcatgctttc aagagaactg aaaaaagtgc aacggcgggt gaacgctctg gagaagatct
    33601 ttattccaca acatgaggag gctaaaaaat acatcaccga caggattgag gaaatggaac
    33661 gcgaggaaat attcgtgaaa aaacttattc gacagcgcca acaacgctaa atcaattcct
    33721 tcagtccgct aataaaagga gatgaactat gaaatcgaaa tcagatgttt tgaaatattt
    33781 ggagctggca gaaagacaag cccgggacaa gaaaaagaaa tcaaacatga aaacatacgc
    33841 ctataaatat ctggaactgg ctgaaaaaca cgcggagaag attgccgcaa gatgggtaaa
    33901 ggacgttaga aacaacaccc gaacgcagac ttataaaaag ctgaatgaaa agcaattagt
    33961 cgatcagtgt acagagtttt atcagcaatt cagtaagatg ttcatttatg aaaaaattac
    34021 ggatgacgtg acgaagtatt ttcagaaata cgcactggaa tgttatgctc ttgacattcc
    34081 cgcgaaagaa gctatttacg cgctgatatt gatgcgacgg aatatctggc tatacgcgga
    34141 atttcaaatg atcttcaact cagctattgc caagcaggaa tccctggata ccctcaaccg
    34201 aactatcctg ctgtttgatt acgcggtgta tgaggtcacc agggaatacc tggaattgat
    34261 gaaaactact tagcggtcga aattcattat tgctgacggt gaatcatgcc atcgattagg
    34321 cacgggtgca tgaggtgctg gtttatgcct ggtgacttcc gcaaccctag aaacgttttt
    34381 ttggcagtgt ctcgaatcgt cattgctttg ccattagaga atcggtggct aagattatga
    34441 caatatgttc tgctaggaga aggcattctt accgcagttt atctggcagt aaaaaattaa
    34501 tgataaaatc gaactaaggc gcataagctc agtcgggtct tttggactcg actgtttttt
    34561 aacgacagcc aagtcaaaaa gacttggctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34681 nnnnnnnnnn ggtcttttgg actcgactgt tttttaacga cagccaagtc aaaaagactt
    34741 ggctgaacaa ggattaaata aatcactaat atttttcaat gtcttccatc aactgattca
    34801 atttcttcgg cgagacgggg tagggggttt taagctcctg agcgaacaga gagaccttgt
    34861 attcttctat catccagaag aattcttcaa ccattgtttt cttttcatcc ggacaatcta
    34921 tggtaatgtt ttcttttaaa agaataattt ctttaaatcc ggttccttta ggctcagtcg
    34981 ggtcttttgg actcgactgt tttttaacga cagccaagtc aaaaagactt ggctggacac
    35041 ggccatttac catctgctgc agtttttgcg aataaattaa aacgtcctgc attttccttt
    35101 gcgccgcgga aagatttaaa tttccccgtt cggctcgcag agcaagcgcc ttgcagtagc
    35161 gcggcagttc cttcatccgt tcaaaggtat aaagctcggg gaaatcgacg ggcacaagac
    35221 gggagatctc tgtttggatg tcttttataa aattcaaaac aggattgttg tttctatttt
    35281 tcgtcatgag tttgccgata acggtacagg tatcggcaca ggctttcaat accggttcga
    35341 tcgctatcaa tacctgctgg ccataaggca gaatttggga attcagacaa ccggcgtgcg
    35401 cgataaattc ctcctgtctt cgccacggct tgtaaaataa atctttcctg acccggttaa
    35461 tgatggattg ttccagttgt ttgggagggc cgatgccggt agcgctggtc ttcattgcgc
    35521 cagtcaaagc gacattcttt ttaagttgtt tcatcttgtc ggcgaagtga atgtcgtaga
    35581 gcgccgcaat accgtgcaaa tgatggaagg cgctttgttt ttcgtcggcg aaaagccgca
    35641 gatcaatgga atcatcgcta atcgtcagcg ccggataggc gtaaccgatc aagccgtgag
    35701 ttccccgaag gggaatctgc tggggcaagt ccccgaaatc ccagccagtg attccctctt
    35761 tttcccactc acctctgatt ttatccatgg ccgatgtact gaccgtaccg gtcagttctt
    35821 tttgcagcag atgaatgtcc cggctttctt taatttcgtt tcctttttca tcaatgacgc
    35881 tgtagcggac attcagataa tccggcagtt tttccgttgc ccaatcatcc ttaggaacct
    35941 ttatcttgta tttgtcgaga agcaactggc tgagcgcctg aggcaaaggc ttgtccaaat
    36001 tcgatttatc ttccaccaaa agctcaacaa ttttagcggg cggcggcaat ttgaggcgaa
    36061 tggatttagg aagcgacttc aataagtgaa cggccttttc ctttaataac gaaggcagat
    36121 ggcgatcgat gtttgctgtc gcggctttgg aaataaaacc cagaggcact ttaacggtga
    36181 cgccgtcatc attttttccc ggttcaaaat tatagcggca gttgaaagcg gcatcgccga
    36241 tttttatccg atcgggatat tgcgatattt cttcagcatc cggttcacgg gcaatcaggt
    36301 cctcttcccg
//
LOCUS       DAOSME010000023        33559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1715_length_33559_cov_19.649624, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000023 DAOSME010000000
VERSION     DAOSME010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 33559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33559
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_33559_cov_19.649624"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1584
                     /locus_tag="PLG98_05150"
     CDS             373..1584
                     /locus_tag="PLG98_05150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOE32673.1"
                     /translation="MALRDAYFVDGVRTWFGKSRQDGHYWTTRADDLVTKVMKELIRR
                     NPKVPWGEVDDNIWGATTQVGDQGTTMGRVTVLTAGLPVEVPGFSVDRMCAGGMTCQA
                     IGSSFVKTGCADIIIAGGVEHMAHHPMGAGADPNPRVVTEKMVEVKYFNMGNTAEKLH
                     DWLPENGEQPVTKEEADEYAYHCLEKYFKALDAGYYHKHCVDMTVFTPNGWKVANYDE
                     QAKRGTTLESIKNFRKFPFKAEGHVTAANASGLNDGACVSLIMSGKRCKELGIQPKMK
                     FVGAGFVGVDPSIMGWGPVPSTEKVLKNFNMKFDDLDIIELNEAFAVQAVGFMKHFGM
                     KLPQDPRLNPYGGTIAMGHPLASSGVRLSMQLAQDFELNPKAKYGLTTMCVGLGMGGS
                     IIWENVVGKKL"
     gene            1597..3678
                     /locus_tag="PLG98_05155"
     CDS             1597..3678
                     /locus_tag="PLG98_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOE32674.1"
                     /translation="MAKYITECKLNFLQSAKAGKIAIVTMDNGQFYNIPNTWGFEAFE
                     FLNKIIDTVEKDKDVKGWILTGKPFIFNVGADIMSVDPTATKEDLLKIGEMGHGTFKR
                     IMNLKIPTLAAINGAAMGGGVEVGLYHNYRTISKSPGGCDHYALPECFLGLIPGWGGT
                     QLVTKLCGPEAAIELIINNPLNMNKMINSIKAVEMGLADRLIAPAEFMDESIALLERI
                     ITGEEKIERKKVDPKMSDELYNKTKEFITGKVHSGAIAPYKALELIKGAAEWSYDEGF
                     QKETEVLADMIMSDQFKCGVYSFDLTQRRAKKLPGRPPKEVKGNKIAKIGVIGAGLMA
                     SQMALLFAQRYQCPVIMKDIKQEFIDKGLAYCREQLAKTAKKGKLSAADAKWIGEDLI
                     SGTLTYEPFKDCDFVIEAVFEEMGIKQKIFGELEAACKPECLFLTNTSSLDVAEMGKN
                     LKDPSRVVGFHFFNPIAVLPLVEIIKTDKTSDVVLATAFDIAKKIKKTPVLVKNAPAF
                     LVNRVLLAWMDGIFQCIDEGADFMQVDNAVFDLGFPMSPFTLAALVGPAIALHVQETL
                     NKSFPDRFPVNAGLKNLVAKGKKQMVTFTDKGIDVDPEIKAGWPQGNKRFTDDEIKAR
                     VLERVAKEVDLILAEGVVAEPKDVDTGVIMGAGWPFFMGGITPYLDQKGISKKVAGKD
                     FHPKGFLTVEK"
     gene            4005..4268
                     /locus_tag="PLG98_05160"
     CDS             4005..4268
                     /locus_tag="PLG98_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32675.1"
                     /translation="MPPAEMRHERVVVETDRYRVEGDITLPQEGYRSRLSDYLNRSDQ
                     EFIILVNVELLSLDGSGRDWRAPVLMLARQHIRLVVPVGGPNA"
     gene            4478..5263
                     /locus_tag="PLG98_05165"
     CDS             4478..5263
                     /locus_tag="PLG98_05165"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOE32676.1"
                     /translation="MNVKDLFDLKGKISVVTGGGRGIGKFIATGLAEAGSDVVIASRK
                     MENLEKEVKELENLGIRALAVKCDLAKKEDIGNLVKAAMDKFGTIDILVNNAGLTWGA
                     PTLDYPLDKWEKIFDVNVKGVWILSQQVARIMKEKGGGKMINVSSIYGSRGSNEEEHP
                     AVAYNPSKFAVEGLTKTLALKLARYKIYVNCIAPGFFKTDMMGHLFKPEMKPILDLTV
                     GAIPLKMYGEEDDVKGLAVFLASKASNYITGGIFPVDGGFSAQ"
     gene            5340..6440
                     /locus_tag="PLG98_05170"
     CDS             5340..6440
                     /locus_tag="PLG98_05170"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOE32677.1"
                     /translation="MKKIKHSFFLLLSLIVVACAPAYTLNHSSPVKPLSLPADAAAHY
                     WAQNEWWYYTGHLKAEDGREFGYELTFFKRITSEDKVPHFFIPIPAHWLKDLGMMGHF
                     VITDITHQKFMTQEVNNFFGLPSKADDTKYDLMIGTWTAREEEGKHLLYAEMDDGRIE
                     LELQPVKVAALHGRQGIVDKGGGNSNYYYSLTNMKTSGTLTIGGESLDVHGKSWMDHE
                     YGTMKLTYPQSGWDWFSIQLENHYELMIYVIRSEGDLKNSLVGGTFVLPNGKTIPVKA
                     RDIEIRNLDYWHSEKTDSDYPSSWEIIVRPFKLKLKCQPVMAEQEVNLQPMPYWEGAI
                     SVSGTFHGKSVKGQGYIELVGYSRKYPIQYIN"
     gene            6578..7327
                     /locus_tag="PLG98_05175"
     CDS             6578..7327
                     /locus_tag="PLG98_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE32678.1"
                     /translation="MSGHSKWSTIKRKKGAIDAKRGKIFTKIIKEITLAARLGGGDIE
                     GNSRLRQAVMLAKEENMPKDNIERAIKKGTGGGEGAAAYEEVTYEGYGPGGAAVLVEI
                     MTDNKNRTVAEIRHIFSKHGGNLGENGCVAWMFAKKGSIVIDKKVIDEDALMELVLEA
                     GAEDVKTEDSEYEVITDPGSFEAVKKILDDKGIKHLEARIGMIPSNTVKLEANKAEQM
                     LKMMEKLEDNDDVQNVYSNFDIDEEVMKKLS"
     gene            7363..7848
                     /gene="ruvC"
                     /locus_tag="PLG98_05180"
     CDS             7363..7848
                     /gene="ruvC"
                     /locus_tag="PLG98_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOE32679.1"
                     /translation="MTLRILGIDPGSHVTGYGVIEKKGNYLKHVVHGEIKPKRNSSLS
                     EILVYIYQQLSSLIAQTVPQAVALENIFYGKNVRSLIKQAQVRGVVIFAGADKDLNIF
                     EYSPLEVKKAVVGYGRAEKRQVQVMVKAILNLPETPPPDAADALATAICHANFLKDKP
                     I"
     gene            7848..8456
                     /gene="ruvA"
                     /locus_tag="PLG98_05185"
     CDS             7848..8456
                     /gene="ruvA"
                     /locus_tag="PLG98_05185"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOE32680.1"
                     /translation="MIALISGTIAYKGISQIIVDVRGVGYRVFIPLTTFYELPETGQT
                     ISLHIHTNVKEDAIHLYGFHTLQERDLFQLMISVSGIGPKVAMNILSGIAAPELLRAI
                     SGGNVSKLVNVPGVGKKMAERLVLELKEKVLKKMAIEEAPEDQTQHLADDLMQEDVLS
                     ALMNLGYRNNVARDALNKVLRTSEAKLSMDKILKETLKILAG"
     gene            8479..9504
                     /gene="ruvB"
                     /locus_tag="PLG98_05190"
     CDS             8479..9504
                     /gene="ruvB"
                     /locus_tag="PLG98_05190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOE32681.1"
                     /translation="MEKSKKNSLISPSCAEEENDFEVTLRPTMLKDYVGQEKVKSNLA
                     IFIEASRKRREALDHVLLYGPPGLGKTTLAYIIAREMNVDIKVTSGPVIERPGDLAAI
                     LTNMHENEVLFIDEIHRLSHVVEEILYPAMEDFHIDILIGQGPSARSMKLDIPRFTLV
                     GATTRAGSLTSPLRDRFGMHFRLEFYSPKELAVIIKRSASILGVKLTASGAEELAGRA
                     RGTPRIANRLLKRVRDYAEVKADGTIDGKIAQEALTAFEVDQKGFDQMDRRLLLTLIE
                     KFNGGPVGIESLAAAIGEERVTIEDVYEPFLIQEGYLQRTARGRIACSRAYEHFGLKA
                     SGQTEIF"
     gene            9539..10081
                     /locus_tag="PLG98_05195"
     CDS             9539..10081
                     /locus_tag="PLG98_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOE32682.1"
                     /translation="MKILMHICCGPCTIYPLKELRTQGHEVTGVFYNPNIHPYQEYKK
                     RYQTLSQYADQIYLKIIWPEDYLMEEFLKGVAFKGKERCSYCLQDRLTFTAQMALQEK
                     YDAFTTTLLYSKYQKHDFIHQAGDNLSQKMGVSFYYQDFRVGWEEGVKISKELEMYRQ
                     PYCGCIYSEKERYYKQNKHS"
     gene            10274..11203
                     /gene="lpxC"
                     /locus_tag="PLG98_05200"
     CDS             10274..11203
                     /gene="lpxC"
                     /locus_tag="PLG98_05200"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOE32683.1"
                     /translation="MNLQRTIKKEINCFSIGLHTGRKVNMKIKPAPADTGIVFIRKDL
                     PEAEPIYARYDNVCDTTLATTLGSNGITVSTVEHLLSAFSGMGVDNAVIELDSFEVPV
                     MDGSALPFVNMLKEVGTHVQKKHKKMLVVKKSVCVNAGDAKAQLLPSEQFKITYEIDF
                     KHPVIGVQSYSMTFSDEEYEKNICAAQTFGFLKDLEFLQARGLALGGSLKNAIVLDDQ
                     KIINKEGQRIPDVFVKHKILDAIGDLFLLGMPIIGHFVAYKSGHRLNNLLLRELISRR
                     DCWEIVTDVNKAENLKSHPFKIPSFRTLDAVQA"
     gene            11363..11932
                     /locus_tag="PLG98_05205"
     CDS             11363..11932
                     /locus_tag="PLG98_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOE32684.1"
                     /translation="MKKIKNKIGLVLIFLFMGAMFFPSQTCALEPKIKDLLITNDAEN
                     VLLYARLLNGFKPQMELAILAGIPTTFTMWIEVYQERSPMWDKKIIRKEIKRSIKYDN
                     LKKTFSVVSEKKDPDIFSDMESAQKAMSDYNGIVAVPMSSLKKGQSYYTLVKIKMDKV
                     RLPLHMEYVFFFVSLWDFETPWYRQNFTY"
     gene            11986..14205
                     /locus_tag="PLG98_05210"
     CDS             11986..14205
                     /locus_tag="PLG98_05210"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32685.1"
                     /translation="MENKPGRSIIDEKELSKRRRERIIMVVVGVLAIIVTLMASQLFN
                     KENLPLSTNIFVYGLTSINFILIVLLMFLIIRNIVKLFYEHRRGVIGSRLRTKLVAAF
                     VGLSLVPTILLFLFAINFLSYSIDFWFNIKIGDALNRSLEVAQVYYQQMEEHAKFNAR
                     QISADITKNRLYEQDRVEYLKSFVTQRQKNYKAGMVETFFDFQQEKLTFADAEDPDIL
                     PVDPTPKMMENIYLGKEESIIHPAEKGDVIVGIVPVFSYAVPTEVIGRVSVSYMIPKG
                     FVDKLRSIADASEQYRKIELLKKPIKLNYMVTLSIVTLVIIFLAIWFGLTLSKGITGP
                     VQDLVQATNRITQGDLDTRIDIDADDEIGTLVKSFNQMTKDLKKSKTSLEEANLNLEQ
                     RRKYMETVLRNVSAGVISVDKTGIITTINRAAEHMFDIRSEKILFRNYRELMIPEHIS
                     LVDEFLQEMKEKNESVLEKQLEIMLQEKALTVLLTITTIQDEEGNDSGIVVVFEDLTL
                     LQKAERAAAWREVARRMAHEIKNPLTPVQLSAQRLQRKYEDKLGEDGSVFKECTQTII
                     DQVEVLKNLVNEFSRYARMPVTSPSPNDLNAVVSEAVSLFQDAHKDIVFDLKTADGLP
                     KFDLDAEQINRVMINLLDNAVAAMNKKNGHIEIAVSYDEQHRRASVSVADDGPGVPSS
                     YKRKVFEPYFSTKKSGTGLGLAIVSSIISDHHGQVNVSDNSPTGTIVSFQLPVSKAS"
     gene            14346..15716
                     /locus_tag="PLG98_05215"
     CDS             14346..15716
                     /locus_tag="PLG98_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE32686.1"
                     /translation="MKETILVVDDEESICQSLKAILSDEGYQVLVAGSGEEAVKVVEE
                     EMPRLVLLDIWLPGMDGLDTLKAIKKIDAQTMVIIMSGHGTIETAVKATKLGAFDFIE
                     KPLSLDKIIILVNNALSLIRLKEENILLKQKVTHQYELTGNSPVIVDLKEMISIVAPT
                     NAWILIMGENGTGKELVARSIHRLSLRADKDIVEVNCAAIPEELIESELFGHEKGAFT
                     GATEKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEKKFERVGGNRLIDMDVRV
                     LAATNKDLEEEMKAGRFRQDLYYRLHVIPLVVPPLRDRKEDIPALAERFILDFTRKEG
                     MEPKILSDDAIELLMKHDWPGNVRELKNIIERMIILTPSNDITAKDIPPLNTKEAKSV
                     FPGTPVAAADSFRDAKMDFERQFILKKLEENDGNISRTAEAIGMERSNLHKKLRSLNV
                     VIKGDA"
     gene            complement(15713..16699)
                     /locus_tag="PLG98_05220"
     CDS             complement(15713..16699)
                     /locus_tag="PLG98_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOE32687.1"
                     /translation="MNKKVGFGILISALLVYLSVRGISLQDVLNDLNKIELTYVILFL
                     FLIILMQYLRSYRWGVILQPLEKIDQVSLFSVTSVGFLAIAAIPARMGELARPYLISQ
                     KSSVKMSSALGTIIIERVLDSFTVLGIAILLLFFIDLPSWMIHSSIIFFLLALAIFCF
                     ILFMILRRKETLQLVNKVLAMLPGRLADKIDELLYNFTNGLQIVTNINLLLYLIFLSV
                     TVWLLDVLAIYVLFLAFGFHLPVIASFVLMIILIVGIAIPTAPGFIGNWHFACVLGLS
                     LFNVAKVEALSFAVVYHFLSMIVVVVLGVIFIPFNKFSISEMKNQINNEKQV"
     gene            complement(16731..17135)
                     /locus_tag="PLG98_05225"
     CDS             complement(16731..17135)
                     /locus_tag="PLG98_05225"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOE32688.1"
                     /translation="MKRNVFLSFISCILIPFLFSGCVPLMVGGAAAGAGAGTYLYING
                     ELKTDYNKSFDQVWKACEKTIAGMHGIDVAPEKEIARGTIQTIINDEKVKFDIAYKAK
                     NLTTVAIRVGVIGNKLSSQLIHDRIADNLVKE"
     gene            complement(17232..18296)
                     /gene="dnaJ"
                     /locus_tag="PLG98_05230"
     CDS             complement(17232..18296)
                     /gene="dnaJ"
                     /locus_tag="PLG98_05230"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOE32689.1"
                     /translation="MTKKDYYDILNVPRTASEDEIKKSYRAIAMQCHPDRNPGNKKAE
                     ERFKEAAEAYEVLSDREKREIYDHYGHEGLNSTGFRGFSGFDDIFSHFSNIFDDVFGY
                     GNARGRSHSYATAGADLRYDLTISFLDAAFGTTTAIDLEKLTTCHHCQGTGAAPGSSP
                     EICRTCRGRGQVIQSSGFFTISSTCPHCNGHGKVISTPCENCRGTGKDRIIKTVQLKI
                     PAGVETGSRLRLRGEGETGSQGGPDGDLYVFINVEEHEFFNRSGDDVICRAPISFVQA
                     ALGGTIEVPTLQGKEKMKIPRGTQNGKTFRLRGKGIPHIRGFGRGDQIVEVCVEIPTT
                     LSKKQEELLIEFEKLNQPTR"
     gene            complement(18572..19453)
                     /locus_tag="PLG98_05235"
     CDS             complement(18572..19453)
                     /locus_tag="PLG98_05235"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOE32690.1"
                     /translation="MIKSIQKTDFHELNLISRGKVRDIYSVGNYMLIVTTDRISAFDV
                     IMPDPVPGKGVILNQMSAFWFEKMKDIIPHHIVSTNPAEFPAETAPYRDSLEGRSMLV
                     KKATPLPVECIVRGYISGSGWKDYQRDRTISGITLPAGLKESSRLPEAIFTPTTKAPE
                     GEHDSPISRKEMADIIGAELTASVINISLAIYQRAASMALKAGIIIADTKMEFGLLDG
                     NLMLIDELLTPDSSRFWPADEYKEGHAQKSYDKQFLRDYLLSINWNQKPPAPQLPSEI
                     IENTRKKYEEALKRLVG"
     gene            complement(19469..20098)
                     /locus_tag="PLG98_05240"
     CDS             complement(19469..20098)
                     /locus_tag="PLG98_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE32691.1"
                     /translation="MFVKQMQVSSMAVFAYIVGDPITGDALVIDPADNIKGIVAEAQK
                     NNLRINYIVNTHGHVDHISGNTEMQKATGAKIIIHQDDAIMLTHTPAMILRMFGAKAS
                     PPADRLVKEGDILSAGNVELRVIHTPGHSPGGICLYTPGFVFTGDTLFVEAVGRTDLP
                     GGSWQELSRAVREKIFTLPDDTKVMPGHNYGSTPTSTVGHEKRYNPFFR"
     gene            complement(20186..21529)
                     /gene="rsmB"
                     /locus_tag="PLG98_05245"
     CDS             complement(20186..21529)
                     /gene="rsmB"
                     /locus_tag="PLG98_05245"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOE32692.1"
                     /translation="MKKTIRHQAVEILNQVHAAHDFAGPLINDCLDKYNLSGTPDGRL
                     LTHLVYGVLRFRGHLDWLLKKLYRGDFAKMQEDIKNVLRVSLYQLRFSERLPDFAVVN
                     EGVEIAKIINPDKSSLVNAILRNFLRRGQNLSFPSEKKNPVEYLAAFHSHPEWMIQTW
                     IKTFGLQETKALCSADNTLPPLTLRVNTLKISRNDLKEKLAAAGFESQTTPYSPNGII
                     LSTAVKPIQKTEFFDKGLLRIQDEGAQLIACLVNPERNESILDACAGAGGKSSHLAAL
                     LENKGFILAFDRNQEKITELKKEIIRLGITIIETQAGNVSAGLPDAFRERFDCVLVDA
                     PCSGLGTLRRNPEIKWRIMQNDILSFAAQQKKILQQASAAVKRGGRLIYCTCSVLPEE
                     NENIVEHFLRVNNQFSLGKPSSTIIRPLTDSSGFFRTFPHRHNMDGFFGALLIRQ"
     gene            complement(21526..22128)
                     /locus_tag="PLG98_05250"
     CDS             complement(21526..22128)
                     /locus_tag="PLG98_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE32693.1"
                     /translation="MNHTMFNTPFVKSLLRGISLLLLKMLGWKTSGKIPREPKFVLIV
                     APHTSNWDLFYGILLAFALKIDARYIAKKELFRGPFSFLMSWTGAIPVERAARHHIVD
                     QMAALLKEKEKCILALAPEGTRHKTDAWKSGFYYIALKAQVPIQLAFIDYARKTGGTG
                     PLIYPTGHPEHDMSMIRDFYQSVRGKHPENTSTVGIHPES"
     gene            complement(22164..23102)
                     /gene="fmt"
                     /locus_tag="PLG98_05255"
     CDS             complement(22164..23102)
                     /gene="fmt"
                     /locus_tag="PLG98_05255"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOE32694.1"
                     /translation="MTNPRILFMGTPAFALPALRLLHGQRYPIAGVVTQPDRPKGRGL
                     KEIASPVKILAQEFGLNVYQPEKVKDPSFMELLQQLHPDMITVAAFGQILPKSVIEYP
                     RLGCLNIHPSLLPRYRGAAPINWQIINGEKKTGVTIMLMDEGMDSGDILLQEESQLDC
                     EENFGELHDRLAKLGAALLIQTIDQVISGTASRQKQETSGVTFAPRLTRETGKILWTS
                     NASDIVNLIRGLSPLPAAYTFMDGLPLKIFSAVARHENVQQPPGTICAIEADSLSVAT
                     ADGYVIVKDVQLAGKKRMPIADFLRGYHLKIGNSLS"
     gene            23333..24481
                     /locus_tag="PLG98_05260"
     CDS             23333..24481
                     /locus_tag="PLG98_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE32695.1"
                     /translation="MFLAATFLFGFSQSIVDSTFNNFLNETYLIDNLQRGILEIPREL
                     PGLLVVAVSAIVFFLCSRRQAALANLLCAFGILLIGFYSPNFSVMLIWLFIFSIGQHL
                     FLPLNQSIGMELAVKGQTGRRLGQFSAVANVAMIIGSFSIFVGFQYFDLDFKTSYFAA
                     FLGFSSVAVLIFMMKKDEPVPVRMKFNLRKEYTLYYWLTILYGTRKQLFLTFAPWVLV
                     TVFHQKTQVVATLLTVGCVIGIFFKPMLGKAIDKLGERLILASEAVILVFVCIGYGFS
                     KSIFTETTALYAVFVCYIVDQMLAAVSMARATYLQKIAVRPEDVSQTLTMGVSIDHIF
                     SIGIAVLGGFIWLKFGYQYLFLTGAVIAVLNFFSALQIKTKTEKLTTP"
     gene            24520..25026
                     /locus_tag="PLG98_05265"
     CDS             24520..25026
                     /locus_tag="PLG98_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32696.1"
                     /translation="MKKIKVTVNDLMQQNYVYYLQEPVGKNFHPQFTPDLTPGKMLEM
                     GVFGGKYMTDCKDEFPKRWFTRAKLCHERHDPDLNYFGVTASQPLSVWREKGWIYHED
                     PRGWFQWYCRYYMGRRCSDDERQIKRWKAMKRHISQIRKHCQKGDINCRPRQRQALLN
                     WAYDSRNI"
     gene            25093..25551
                     /locus_tag="PLG98_05270"
     CDS             25093..25551
                     /locus_tag="PLG98_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HOE32697.1"
                     /translation="MTETWLRITNPDNPPNDAMLSAWIGNKAYKYLQQVTKLIVALYP
                     DVFVPEWIYGGKKHGWSLRYKKSKSFCTLIPEKSRFALLIVFGAQERANLETVKDRLS
                     QHTRRDYDRAATYHDGKWLLLPVDNDQVVEDLKILLAVKRKPLTAECIIK"
     gene            25684..26466
                     /locus_tag="PLG98_05275"
     CDS             25684..26466
                     /locus_tag="PLG98_05275"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOE32698.1"
                     /translation="MTINIKTEEHIMLIEIDRPQKYNAMTREMYTQLARAYYRLDRDD
                     DLWVGVVYANGPHFTSGLELTDWSDVFGSGAGFPLSGENEIDPFYMMNEERCRKPLIY
                     AVQGYCFTWGPEVMLNADIRVAASDTKFAMLEVRRGFFPCGGATIRLARDIGWSNAMR
                     YLLTGDQWSAEEAYRMGLVQYVTETGKQLEKAMELARKVASAAPLGVRNILQNARLAE
                     REGEQAAKKVIYQEVSKVMKSEDMKEGLQSFIERRAAVFKGK"
     gene            26523..27095
                     /locus_tag="PLG98_05280"
     CDS             26523..27095
                     /locus_tag="PLG98_05280"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOE32699.1"
                     /translation="MDPTFFNAGVFKFVILPLLIFSTRICDVTLDTLRIIYVSRGLKL
                     LAASIGFIEVLIWLFAITQIFKNLTNPIYYVAYAGGFAMGNYVGILIEEKMAVGTVVI
                     RTITQKDAGVLIKWLKAEGYGVTYIDAQGAFGPVKVLFTIVKRKDIDHVLGMIRTCNP
                     LAFYTIEDIRSSSKGVFPIVKSRKNRYKEP"
     gene            27171..27374
                     /locus_tag="PLG98_05285"
     CDS             27171..27374
                     /locus_tag="PLG98_05285"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOE32700.1"
                     /translation="MECKKEKNLDNCNCTYEPCSRKGACCDCLSYHLKQRQLPACCFP
                     KDAEKTYNRSFEYFTELVSQRRI"
     gene            complement(27502..28497)
                     /locus_tag="PLG98_05290"
     CDS             complement(27502..28497)
                     /locus_tag="PLG98_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOE32701.1"
                     /translation="MDHSIKIEDLNDPVMKHASTDFVSIYSDNTVGEALDCIRNCQTQ
                     STILYFYVLDGKARLVGTIQTRRLLTSPLDVKIEEIMSKNLVVIPASATLVEALEFFI
                     FHRYLAFPITDDHEKMLGVIDINVFTEEMIDIEEQGQVHSVFDTLGIKISEVRHKSPW
                     SVFRYRFPWLLATITSGTVCALLVGFFEATLAQSLVLAFFLTLVLGLGESVSMQTMAI
                     TVHSMHHSKAESGWYFQTMQRELTRTFLMALACAFIVGFIVVVWKNDFTAGLIISSGI
                     LTSLLFACFLGVSLPYSLHKLKLDVHVASGPLTLALADICTVIVYFSLAAFILSS"
     gene            complement(28854..30581)
                     /locus_tag="PLG98_05295"
     CDS             complement(28854..30581)
                     /locus_tag="PLG98_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE32702.1"
                     /translation="MDELSVLITFAVGLAAALFFGYIAVRLKLSPIIGYLLAGIIVGP
                     FTPGVVVNRTIAEQFAEIGVILLLFGIGLRFHLRELIAVWRLAVPGALIQSTLSTSAL
                     AVLLHFMGWDWASGFILGMAISVASTVVMAMVLTEWHDLHAKIGHIAIGWTVVEDILT
                     VALLLLLPIIFSPDRSMDQNIGAVLGLAGIKIVGLLAIVVVLGKWIIPWALERIAKTR
                     SRELFTLAVLVLAVGIAVGSAKLFGVSMALGAFLAGLAVGRSEFAARAGIDALPMRDA
                     FAVLFFVSVGMLFDPLSLIQVPFVIATVLLVVVIIKPLAAALTVRMLGQPLAAAIPIG
                     AAFSQVGEFSFILGTVALQLKIINDAGWNALVAASIISIALNPYVYRWARRLSSKTNK
                     ALPVVKSQPQTVNPSHCILIGYGPIGQIVHNLLTERGNEITVIDLNLKSVRQLNAQGY
                     KAIYGDVLRPGTLEDAGIATAGSLIISTDIEDASEIIKNARLLNPDLQIMVRCAHLRD
                     AAALRQAGATVVAAGEAEVGVALSEVVTAADQMACVMAAEHRETVRRILYDKAFQQNE
                     TVSKKTRKK"
     gene            31019..31951
                     /locus_tag="PLG98_05300"
     CDS             31019..31951
                     /locus_tag="PLG98_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE32703.1"
                     /translation="MKQFKSILYVTGENGDQQSAIARAVSLAENNQADLTVIDVIPTV
                     MGEYSEEIMSSRMQFLESFTEPYNKRLDIRISVETGTVFIEIIREVLRNKHNLVIKPA
                     ENPDFLKRLFGSTDIHLLRKCPCPVWIMKLPEKSNYSCIMAAVGFKPLETPETEENLN
                     REILDLASSLALSDFASLHVVHAWEAFAAHYIRSRGDKEHEAMAVYVEKERSLHQNAL
                     NVLMEKLSKRVGDDVYQELSPSLHLLQGDAQKMIAALAAKLQADLVVMGTIARTGISG
                     LIIGNTAEAILDQLECSVLAVKPPGFVTPVKLEE"
     gene            31988..32512
                     /locus_tag="PLG98_05305"
     CDS             31988..32512
                     /locus_tag="PLG98_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32704.1"
                     /translation="MKRLSSKSTFFIKRIFPPLWFGCIIFFALMALSAESKGIHVDDI
                     FIVLLAAIFMVLIGYFIMRHLVFDLIDEAYDEGASLLFKNNGQSVRVDLTAIKNVSYT
                     VLINPPRVTLSLRHETQFGDELTFSPSGSFIPFKKNEEILGLIDRIDRARGGSDNTLP
                     IQEGQICKENSTNC"
     gene            32485..32898
                     /locus_tag="PLG98_05310"
     CDS             32485..32898
                     /locus_tag="PLG98_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32705.1"
                     /translation="MQRKFNELLIIGLGGTIFFGSFFAGEYLGASESNKDSWWTPMTM
                     ALSLDQTRPEFELYLKKELLQKHIEKGTLLVANDGENLSKLVLGDIKIRLNNWNKVKA
                     EKLKYAVITAFFLGASIALLIIGLMRFLADKEDAQ"
     gene            33057..33365
                     /locus_tag="PLG98_05315"
     CDS             33057..33365
                     /locus_tag="PLG98_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32706.1"
                     /translation="MKTVDGVLEIHGGFVTWFLKHGLPVRGYIGALTLGHVVLGYNEN
                     LLSAYRRHEHAHVKQYEMLGPLFLPVYLAASIWAWLRGRGAYKGNYLEKKAKEHSREF
                     "
BASE COUNT         9113 a         7429 c         8446 g         8571 t
ORIGIN      
        1 gtgctacctt cacacggtag aagtcactgg ttcaatccca gtaccgccta ccaatgaaat
       61 caaggggttg tggtttaggc tgcgccccct tttcttttct gcatcatcat taaatgccgg
      121 ttttggaaaa gatcatctga tcttttgaga atcctcgttt gcggcaatcc gggatggttg
      181 caaaaaaagt tgttattagt cagaagttga ttgacaaaac ttccgctgtg atgctataaa
      241 acaacagctt aaggatcaag aggtcttacc gtagttggtg atcatttatc cctatctgaa
      301 taactattgg tggtcattta ttcctatctc aataattaaa agaacaccga tataaagcaa
      361 ggaggatgtt ttatggcttt aagagatgcg tattttgttg atggagtgcg tacatggttc
      421 ggaaagtcca gacaggacgg ccattactgg accacccgcg cagatgattt ggtgacgaaa
      481 gtcatgaaag aacttattag aagaaacccg aaggttccat ggggcgaagt tgacgacaac
      541 atctggggtg cgacaacgca ggttggggat cagggtacta cgatgggaag ggtaacggtt
      601 ctcaccgcag gtcttccggt agaggttccc ggattttcgg tggacagaat gtgcgcgggc
      661 ggtatgacct gccaggccat cggttcatct ttcgtcaaga cgggctgcgc cgacattatt
      721 attgcaggcg gtgtggagca catggctcac catcccatgg gagcgggcgc agacccgaat
      781 ccgcgggttg tcaccgagaa gatggtcgag gtcaagtatt tcaatatggg caacacggcg
      841 gaaaaactgc acgactggct tccggaaaac ggcgaacagc cggtgacgaa agaagaagcc
      901 gatgaatatg cctaccattg tctggaaaaa tatttcaagg ctttggatgc aggctattac
      961 cataagcact gtgttgatat gacggttttt acccccaatg gatggaaagt tgccaattat
     1021 gatgaacaag ccaagcgcgg aacgacgctt gagagcatca aaaatttccg gaaattcccc
     1081 ttcaaggcgg aaggccatgt caccgcggca aacgcttcgg ggttgaatga cggcgcttgc
     1141 gtttcactca tcatgtcagg caagcgatgt aaagaactgg gcattcagcc caagatgaaa
     1201 tttgtcggtg ccggtttcgt tggcgtggat ccgtccatta tgggatgggg tcctgttcct
     1261 tccacggaaa aggttctcaa gaacttcaat atgaaatttg atgatctcga tatcatcgaa
     1321 ttgaacgaag cattcgctgt ccaggctgtc ggtttcatga agcactttgg catgaagcta
     1381 cctcaagacc cacgccttaa cccatacggc ggaaccattg ccatgggcca tccgctggcc
     1441 agctccggtg ttcgcctgag catgcaattg gcgcaggact tcgagcttaa tccgaaggcg
     1501 aagtacggac tgaccaccat gtgtgtcggt ctcggtatgg gtggttctat catttgggaa
     1561 aacgtcgtag ggaaaaaatt ataggaggtt aaggctatgg caaagtatat aacagaatgt
     1621 aaactcaatt tcttgcagtc agcaaaggct gggaaaatag cgattgttac aatggacaac
     1681 ggccagttct acaatatccc caatacgtgg ggttttgagg cctttgaatt tctaaacaaa
     1741 attattgata ccgttgaaaa agacaaggat gttaaaggct ggatcttgac tggcaagcct
     1801 ttcatcttca atgtcggcgc cgacattatg agtgtggatc cgactgcaac aaaagaggat
     1861 cttctcaaaa tcggtgagat gggacatggt acgttcaaaa gaatcatgaa tctcaagatc
     1921 ccgacactgg ccgccattaa tggcgcggcg atgggcggcg gcgtcgaggt cgggctgtat
     1981 cacaattacc gcacgatctc caagagcccg ggcggatgcg atcattatgc gctgcccgag
     2041 tgcttcctcg gtttgattcc cggttggggc ggaactcagc tggtaaccaa actttgcggt
     2101 ccggaagcag ccattgagct catcatcaac aacccgctca atatgaacaa aatgatcaat
     2161 tctataaaag ctgtcgagat gggtctggcg gatagactca ttgctccggc cgagttcatg
     2221 gatgaatcca ttgctttact ggagagaatc attaccggcg aggagaaaat tgagcgcaag
     2281 aaagtggatc cgaagatgag tgatgagctc tacaataaga caaaagaatt cattaccggt
     2341 aaggttcatt caggcgccat tgcaccttac aaagctctcg agttgatcaa gggtgcggcg
     2401 gaatggtctt atgatgaagg gttccagaaa gagactgaag ttctggctga tatgattatg
     2461 tccgatcagt tcaagtgcgg tgtttactct ttcgatctga cccagcgccg cgccaagaaa
     2521 cttcccggca gaccgccgaa agaagtcaaa ggcaacaaga tcgccaagat cggcgttatc
     2581 ggcgccggcc tgatggcttc ccagatggcc ctgttattcg cacagcgtta tcagtgcccc
     2641 gttatcatga aagacatcaa acaggaattt attgataagg gcttggctta ctgcagggaa
     2701 cagcttgcca agaccgcgaa aaaaggcaaa ctgtccgcag cggatgccaa gtggataggc
     2761 gaagatctta tttccggcac cttgacctat gagccgttca aggattgtga tttcgttatc
     2821 gaagcggttt tcgaagaaat gggcatcaaa cagaaaattt tcggcgaact ggaagcagcc
     2881 tgcaagccgg aatgcctgtt cctaaccaac acatcctctc tggatgttgc tgaaatgggc
     2941 aaaaatctga aagatccttc ccgcgtggtc gggttccatt tctttaatcc catcgccgtg
     3001 ctgccgcttg tcgagatcat caaaaccgac aagaccagcg atgtcgttct ggcaaccgca
     3061 tttgacattg ccaagaagat caagaagaca ccggttctgg tgaagaatgc ccctgcattc
     3121 ctggtcaaca gagttttact ggcctggatg gacggtatct tccagtgcat cgacgaaggc
     3181 gccgatttca tgcaggtgga caacgcagta ttcgatctgg gcttccccat gtcgccgttc
     3241 acactggctg cgctggtggg ccctgctatc gcactgcatg ttcaggaaac cctcaacaag
     3301 tctttccccg accgattccc ggtcaatgcc ggactcaaga acctggtggc gaagggcaag
     3361 aaacaaatgg ttacctttac ggacaaaggc atcgatgtcg atcccgagat caaagccggt
     3421 tggccgcagg ggaacaagag attcaccgac gacgagatca aggcgcgtgt gttggagcgt
     3481 gtagccaaag aagtcgactt gattcttgcg gaaggtgtcg tggcggaacc gaaagatgtg
     3541 gataccggcg tcatcatggg agcgggctgg ccgttcttca tgggtggcat taccccatac
     3601 ctggatcaga aaggaatttc gaaaaaggtt gcgggaaaag atttccatcc gaaaggtttc
     3661 ttaaccgtag agaaatagta gcactctaat caagaaatga aattgccggg cgacccttat
     3721 ggttgcccgg ctttttttat gttttgccat ccgatgaaaa aagcaaagac aaaacattaa
     3781 acatgttttt ctcaggcttg ttcatgaaca gtcaacttat tttccggaac ccctttttta
     3841 atcaatttta tatttttgac aatgtgctgt tgaatatttc attgacttac acattcaaat
     3901 tttctataat tttattagaa atggtggaaa ttttaaaaag tttgcgacga tcattggctg
     3961 attgataaaa acatatctga tattgcgttg cggaggtcat agtaatgccg cctgcggaaa
     4021 tgaggcatga aagagttgta gtggaaacgg accgctatcg cgtggaagga gatataacac
     4081 tgcctcagga agggtatcgc agccgccttt ccgattattt aaaccgaagt gatcaggaat
     4141 ttatcatcct tgtaaacgtt gaattgttat cgcttgacgg gtccggccgc gattggcgtg
     4201 ccccggtgct tatgcttgcc agacagcata tcaggctggt tgtccccgtg ggcggaccta
     4261 atgcttaagg tgagagaaat cgtgaatggc cgctcttttg cagaacgttg aaaatcattc
     4321 cttttaaatt catccggcat tggaacagtt tatctgaaac ccgtacaatg acatttatct
     4381 tccataaaat aataaccgca gatgttgtaa aaacgatcat ggcaggatcg taaagaatat
     4441 tttcaatgaa taaaaaacat gtgaaggagt ttgtaaaatg aacgtcaaag atttgtttga
     4501 tttgaagggc aagatttccg tcgtgacagg cggcggcaga ggcatcggta aattcatagc
     4561 gacggggctt gcggaagccg gttcagatgt cgtcattgcg tcgcgcaaga tggaaaacct
     4621 cgaaaaagaa gtaaaggaac tggaaaatct gggtatccgg gctctggcgg tgaaatgcga
     4681 tttggccaaa aaagaagata tcggcaatct ggtcaaagcc gccatggata aattcggcac
     4741 gatcgacatc ctggtcaata atgccggact gacatgggga gcgccgacgc tggattatcc
     4801 gctggataag tgggaaaaaa tatttgacgt taatgtcaag ggcgtgtgga ttctttccca
     4861 gcaggttgcc cggattatga aggaaaaagg cggcggcaag atgatcaatg tgtcttccat
     4921 ctacggttcg cgcggctcca atgaagaaga gcatccggcc gttgcttata atccgtccaa
     4981 atttgccgtg gaaggcttga ccaaaacgct ggcccttaaa ctggcccgtt acaagattta
     5041 tgtgaattgc attgctccgg gttttttcaa aacggatatg atgggccatt tattcaagcc
     5101 ggagatgaaa cccattctcg atttgacggt cggcgctatt ccgctcaaga tgtacgggga
     5161 agaagacgat gtaaagggat tggccgtatt tctggcatcc aaggcgtcca attacattac
     5221 cggcggtatt ttccccgtgg acggcggatt ttccgcgcag taaaaagtcc ggatgggttt
     5281 gcgaaagagg catgacgccg aagatttcac gggctttgaa aagtcatgag gtaaaaaaca
     5341 tgaaaaaaat aaaacattcg tttttccttt tgctgtcttt gattgtggta gcctgtgctc
     5401 ccgcttatac gctcaatcat tcttcaccag tcaaacccct cagtctgccc gctgacgccg
     5461 ccgctcatta ctgggctcag aatgaatggt ggtattacac aggacatttg aaagcggagg
     5521 atggaaggga attcggatat gaattgacct tcttcaagcg aattaccagt gaggataagg
     5581 ttccgcattt ttttattccc atcccggcgc actggctcaa agatcttgga atgatggggc
     5641 attttgttat taccgacatt acccatcaga aatttatgac gcaagaggtg aataatttct
     5701 tcggcttgcc gtcaaaagcg gacgatacaa aatacgatct catgatcggc acctggacgg
     5761 cccgtgaaga agaagggaaa catcttctgt acgctgaaat ggatgacggc cggatcgaac
     5821 tggaactgca gccggtaaag gtcgccgccc tgcatggacg acagggcatc gtcgataaag
     5881 gagggggcaa ttccaattat tattattcat tgacgaacat gaaaacgtcc ggcactttaa
     5941 ccatcggggg cgaatcgctt gatgtgcatg gaaaatcatg gatggatcat gaatacggaa
     6001 ccatgaagct cacttacccg cagagcggat gggattggtt cagtattcag ctggagaacc
     6061 attacgaatt aatgatctat gttatcagaa gtgaaggcga tctgaaaaac agcctggtcg
     6121 gtggaacctt tgttctgccg aatggaaaaa ccattcccgt caaagcgcgg gatatcgaaa
     6181 tcaggaatct ggactactgg cacagtgaaa aaaccgactc cgattatccg tcttcatggg
     6241 aaatcattgt caggcctttc aagctaaaac tgaaatgtca gccggttatg gctgagcagg
     6301 aagtcaattt acaacccatg ccctactggg aaggggccat atccgtcagc ggaacgtttc
     6361 atggcaaatc cgtcaaaggt caagggtata ttgaactggt gggctattcc agaaaatatc
     6421 caatccaata cattaattaa aatgattccg ccatggaacg ataaacgaac ctttattttt
     6481 accatgaaat tattcttcca cataaggcaa taggctttac aagcctattg ccttatgtta
     6541 ttagaaaaac gatgaaaaaa ccattaagga gaactgtatg tccggccatt ccaaatggag
     6601 cacgattaaa agaaaaaagg gtgcgataga tgccaaacgc ggcaaaatat ttactaaaat
     6661 tatcaaggaa ataactctgg cggcaaggct gggcggaggg gatattgaag gcaactcgag
     6721 gttaaggcag gctgtcatgc tggccaagga agaaaatatg cccaaggata atattgagcg
     6781 ggcgatcaaa aaaggtaccg gcggcggcga aggcgcggcg gcctatgaag aagtcaccta
     6841 tgaaggttac ggaccgggcg gtgcggcggt tcttgtggaa atcatgacgg ataataaaaa
     6901 cagaactgtt gcagaaatca gacatatctt ttcgaagcat ggcggcaatc tcggtgaaaa
     6961 cggctgtgtt gcgtggatgt tcgccaagaa ggggagtatt gttatcgaca agaaagtgat
     7021 tgatgaagat gctctgatgg aactggtttt agaagccggg gcggaagatg tgaaaacgga
     7081 ggacagcgag tacgaagtga ttaccgatcc cgggtccttt gaagcggtca agaagattct
     7141 ggatgacaag gggattaaac atctggaagc caggatcggc atgattccgt ctaataccgt
     7201 aaaactggaa gccaacaagg cggagcagat gctgaagatg atggagaaac tggaagataa
     7261 cgacgatgtc cagaacgtat attccaattt cgatattgat gaagaggtca tgaagaagtt
     7321 gagttgagat ccataaaaac catttttaaa aatatggaat gaatgacatt gcgaattctg
     7381 ggtatagatc cgggcagtca cgtcaccggt tacggcgtca ttgagaaaaa aggaaattat
     7441 cttaagcacg ttgttcatgg tgaaataaaa ccgaaaagaa actcgtcttt gtcggaaatc
     7501 ttggtatata tttatcagca gctttcctct ctgattgcgc agacagttcc ccaggctgtt
     7561 gcgctggaaa atatttttta cgggaaaaat gtacgcagtt tgattaaaca ggcgcaggtg
     7621 aggggcgtgg tgatttttgc cggcgcggat aaggatttga atatttttga gtattcgccg
     7681 ctggaagtca aaaaagcggt tgtgggctac ggcagagcgg agaagcgaca ggtacaggtg
     7741 atggttaagg ctattttaaa tctgccggaa acgcctcccc cggatgcggc ggacgccctg
     7801 gcaacggcca tatgccatgc caatttttta aaggacaaac ccatctgatg attgctttaa
     7861 tcagcggaac cattgcctac aagggcatct ctcagattat tgtagacgtg cggggcgtcg
     7921 gttatcgggt ttttattcct ctgacgactt tttatgaact gccggaaacc ggccagacca
     7981 tttcgctgca tattcatacg aacgtcaagg aggatgccat tcatttgtac ggttttcaca
     8041 cgcttcagga aagagacctt tttcaactga tgatttccgt cagcggcatc gggccgaagg
     8101 ttgccatgaa tattctttcc ggcatcgcgg cgccggaact gctcagggcc atctccggag
     8161 gcaatgtcag taaactggtg aatgttccgg gcgtcggtaa aaaaatggcc gagcgtcttg
     8221 tcctggagtt gaaggaaaag gttctgaaaa aaatggcaat cgaagaggct cccgaagatc
     8281 aaacgcagca tcttgcggat gatctcatgc aggaagatgt cctttccgct ttaatgaatc
     8341 tggggtatcg aaacaatgtt gcccgggatg cgctcaacaa ggttctgcgc acatccgaag
     8401 caaaattgag tatggataaa attctgaaag agacattgaa aattctagcc gggtaaaaag
     8461 acgtataagg aatcagcgat ggaaaaatcg aaaaaaaatt ccctcatcag tccttcatgc
     8521 gcggaagaag aaaatgattt tgaagtcacc ttaagaccga ccatgttaaa agattatgtc
     8581 ggtcaggaaa aggtgaaaag caatctggcc atattcatcg aagcctccag aaaacgccgg
     8641 gaggcccttg accatgtcct tctttacggt cctccgggac tcggaaaaac gacgctggcc
     8701 tatattatcg ccagagaaat gaacgttgat atcaaggtca catcgggacc ggttatcgaa
     8761 agaccgggag atctggcggc gattctgacg aatatgcatg aaaatgaagt gctttttatt
     8821 gatgagattc accgtctttc ccacgtcgtg gaggaaattc tgtatccagc catggaagat
     8881 tttcacatcg atatcttgat cggacaggga ccgtctgccc gatccatgaa actggatatt
     8941 ccccgattca ctctggtggg ggcaacgacg agagcgggat cgctgacctc gcccctgcgg
     9001 gatcgtttcg gcatgcattt tcgtctggaa ttctattcgc cgaaggaact ggccgtaatc
     9061 attaaacggt cggcctccat tctgggtgtg aaactgacgg cttccggcgc cgaggaattg
     9121 gccggacgtg cgcgtggcac accccgtata gccaaccgct tgctcaagcg cgttcgcgat
     9181 tatgccgaag tgaaggcgga cggaaccata gacgggaaaa tagcgcagga agcgctgact
     9241 gcttttgaag tcgatcagaa agggtttgat cagatggaca gaaggctgct gttgacgctg
     9301 attgagaaat tcaacggcgg tccggtcggt atcgagagtc ttgccgccgc cattggagaa
     9361 gaaagggtca ccattgaaga tgtctatgaa ccattcctga ttcaggaagg ttatctgcaa
     9421 aggacggcac gcggacggat cgcctgttcc agggcttatg aacatttcgg cctgaaggct
     9481 tccggacaga ctgaaatttt ttaagcggac aacgtatcgc aacggatgtt gttgtaagat
     9541 gaaaatatta atgcatattt gctgcggacc ctgcacgatt tatcctctga aagaattaag
     9601 aacacaggga cacgaagtca ccggtgtttt ttacaatccc aacattcacc cgtatcagga
     9661 atacaaaaag cgttatcaga ccttaagcca atacgcggat caaatatatt taaaaatcat
     9721 ctggcccgaa gattacttga tggaagaatt tttaaagggc gtcgctttca aaggaaaaga
     9781 aaggtgctct tactgtctgc aggatcgtct gacgtttaca gctcagatgg ccttgcagga
     9841 aaagtacgat gcgttcacaa caacattgtt atacagcaag tatcagaaac atgatttcat
     9901 ccatcaagcc ggagataatc tcagtcaaaa gatgggagta tctttttact atcaggactt
     9961 ccgcgtgggg tgggaggaag gcgttaagat atccaaagaa ctggaaatgt accgtcagcc
    10021 gtattgcggt tgcatttaca gtgagaaaga acggtattac aaacagaata aacattcctg
    10081 agatcaattg ttttttcata atcgctgtgt caaatttgcc acactttaat cgaaataagt
    10141 tggtcttgct gcgtattgtc tttaaatagt tcaataatta caatgatttc ttttttaata
    10201 aatatcatca aatatggcat gttctttgcc tttgataaat ttgaagaaag attagatatc
    10261 ctggcataaa attatgaact tacaacgtac aatcaaaaaa gagataaatt gtttcagtat
    10321 cgggctgcat acagggcgaa aggttaatat gaagattaag ccggcacctg cagatacggg
    10381 gattgttttt atccgcaagg atcttcccga ggctgaaccg atttatgcgc ggtatgacaa
    10441 tgtttgcgac acgaccctcg ctacaacgct ggggtccaac ggtattaccg tttcgacagt
    10501 ggagcatctt ttgtcggcat tcagcggcat gggtgttgat aacgcggtca ttgaactgga
    10561 ttcgtttgaa gtcccggtga tggacggaag tgcgcttccg tttgtgaata tgctcaaaga
    10621 agtgggaacg catgtacaaa agaaacataa aaaaatgctg gtggtaaaga aatccgtttg
    10681 tgtgaatgcg ggagatgcaa aagcgcagtt actgccctcg gaacaattta agatcaccta
    10741 tgaaatcgat ttcaaacatc ccgtcatcgg ggttcaatcc tacagcatga ctttttctga
    10801 cgaggagtat gaaaaaaaca tttgtgcggc tcagaccttc ggatttttaa aggacctgga
    10861 gtttttgcag gccagaggtc tggcattggg cggatctctg aaaaatgcca ttgtgcttga
    10921 tgatcaaaag atcattaata aagaaggaca aagaatccct gatgtttttg tgaaacataa
    10981 aatccttgat gccatcggcg atttgttttt gctggggatg ccgattatcg gtcattttgt
    11041 cgcgtataaa tcaggccacc gtttgaacaa cctcttactt cgggaactga tcagcagaag
    11101 agattgctgg gaaatcgtca ccgatgtaaa caaggcagaa aatcttaaaa gccatccatt
    11161 caaaattcct tcatttagaa ctctggatgc cgttcaggcg taatccaaat tcatcttgct
    11221 ttttatggtc atgttgccta gtattgcacg ttgatccgcc aaatgagctg aaccgttatt
    11281 tatcggttac ttggatcgga tcatcgatgc tgtttaattt tgcagccgca acgggtcctg
    11341 ctgcgtgggc tgatgaattg gtatgaaaaa aataaaaaat aaaatcggtc tggtattgat
    11401 ttttcttttc atgggcgcga tgtttttccc gtcacagacg tgcgctctgg aaccgaaaat
    11461 taaggatctt ctgattacca atgatgcaga aaatgttctt ttatatgccc gtctgctgaa
    11521 cggtttcaaa ccgcaaatgg aactggcgat tttagcaggc attccgacga cgtttaccat
    11581 gtggattgaa gtgtaccaag agcgatcccc catgtgggac aaaaaaatca tccggaagga
    11641 aattaaacga tcgatcaaat atgataacct gaaaaaaacc ttcagcgtcg tcagtgaaaa
    11701 gaaggatccg gacatttttt cggatatgga aagtgcgcaa aaagcgatgt ctgactacaa
    11761 cggtatcgtt gccgttccca tgtcgtcttt aaaaaagggg caaagctatt atacgctggt
    11821 taaaataaaa atggacaagg ttcgtttgcc gctgcacatg gaatatgtct ttttctttgt
    11881 ttcgctgtgg gactttgaaa cgccctggta caggcaaaac tttacttatt aacacggatc
    11941 agttttgtca tgatatgaat gatttcaaaa ccgttactgt gatctatgga aaataaacct
    12001 ggcagaagca ttattgacga aaaagaatta agtaaacgac gccgcgaacg aattattatg
    12061 gtggtcgtcg gcgttctggc cattattgtt accctgatgg ccagtcagct gttcaacaag
    12121 gaaaacctgc ctctttcaac gaacattttt gtttacggcc tgaccagtat caatttcatt
    12181 ctcattgttc tgttgatgtt tcttattatc agaaacattg tcaaactgtt ttacgaacac
    12241 cgccgcgggg tcatcgggtc ccgcttaaga acaaaactgg tagccgcatt cgtcggcctg
    12301 tcgcttgttc caaccattct gctttttttg tttgcgatca attttctttc ttacagtatt
    12361 gatttctggt tcaacattaa aatcggcgat gcattaaacc gttcgctgga agttgctcag
    12421 gtttattacc agcagatgga agagcatgcc aagttcaacg cccgtcaaat cagtgcggat
    12481 ataacaaaaa accgccttta cgaacaagat cgtgttgaat atctcaaaag cttcgtcacg
    12541 caacgtcaaa aaaactacaa agcggggatg gtggaaacat ttttcgattt ccagcaggaa
    12601 aaactgacgt ttgccgatgc ggaggatccg gatatactgc cggtggatcc cacgccgaaa
    12661 atgatggaaa acatttatct gggcaaggaa gaatcaatca tccatccggc ggaaaagggc
    12721 gatgtgattg tcggcatcgt gccggtattt tcctatgcag tgccgacgga agtcatcggc
    12781 cgggtgtcgg tgagctacat gatccccaag gggtttgtcg ataaattaag aagcattgcc
    12841 gatgcgtcgg agcaatatcg caaaatcgaa ctgctgaaaa aacccattaa actcaattat
    12901 atggtcacgc tgtcgatcgt gacgctggtg atcatatttt tggccatctg gttcggtttg
    12961 acattgtcaa aaggcattac cggccctgtg caggatctgg tgcaggcaac caacagaatc
    13021 acgcaaggag acctggacac ccgcattgat attgacgcgg atgatgaaat agggacgctg
    13081 gtgaaatcat tcaatcaaat gacgaaagat ttgaaaaaga gtaaaaccag ccttgaagaa
    13141 gccaatctga atctggaaca acggcgcaaa tacatggaaa ccgtgttgcg caatgtttcc
    13201 gccggggtga tttccgtgga taaaacgggt atcattacga cgatcaaccg tgcggcggaa
    13261 cacatgtttg acatccggtc ggagaaaatt ctgtttcgca attaccggga actgatgatt
    13321 cccgaacaca tatcgctggt ggatgaattt cttcaggaaa tgaaggaaaa aaatgaaagc
    13381 gtgctggaaa aacagctgga aatcatgctg caagagaaag cgctgaccgt tttgctcacg
    13441 atcacaacca ttcaggatga ggaagggaat gattccggta tcgtcgttgt tttcgaggat
    13501 ttgacattgc tgcaaaaagc ggagcgcgct gcggcatggc gtgaagtggc ccggcgcatg
    13561 gcgcatgaaa ttaaaaatcc tctgacgccg gttcaactct ccgcacagcg gcttcagcgg
    13621 aaatatgaag ataaactcgg ggaagacggt tccgtgttca aagaatgcac gcagaccatt
    13681 atcgatcagg tggaggtatt gaaaaatctg gtgaatgaat tctcgcgtta cgcgaggatg
    13741 ccggttacct cgccgtctcc caatgatttg aatgctgtgg tctccgaagc cgtctcgctg
    13801 tttcaggatg cgcataaaga catcgtcttt gatttgaaaa cagccgatgg tttgccgaaa
    13861 ttcgatctgg atgcagagca gatcaaccga gtcatgatca atcttctgga taacgctgtc
    13921 gcggcgatga ataagaaaaa cggacacatt gaaatcgccg tcagttatga tgaacaacat
    13981 cgccgtgctt ctgtttccgt cgccgatgac ggtccgggag taccttccag ctataaacgg
    14041 aaggtctttg agccttattt ttccaccaag aaatcgggaa ccggactggg gttggctatt
    14101 gtcagttcga tcatttccga tcatcatggt caggtgaatg tcagcgataa cagccccacg
    14161 ggaacgattg tctctttcca actgccggtt tcaaaagcgt cgtgagactc acgtctgata
    14221 aggcccgggc agcggaaaca gtcaccggaa gattgcggaa cgtcgcagcg catgtacccg
    14281 aaggcttgcg ttacggttca ttcccgtgat atataaaaaa aagtttacgc gtagaaagag
    14341 tgagcatgaa ggaaaccatt cttgttgttg atgatgaaga aagcatctgt cagtccctga
    14401 aagcaattct cagcgatgag gggtatcagg ttctggttgc cggcagcggt gaagaagccg
    14461 taaaagtggt cgaagaggag atgccgaggc tcgttcttct cgatatctgg cttccgggga
    14521 tggacggtct ggacacgctg aaagccatca agaaaatcga tgcccagacc atggtcatta
    14581 tcatgtccgg tcacgggaca attgagacag ccgtgaaagc gacaaaactc ggcgctttcg
    14641 attttattga aaagcctctt tcgctcgata aaatcatcat cctggtcaat aatgccttaa
    14701 gtctgatccg tcttaaagaa gaaaacattt tattaaaaca aaaagtaacc catcagtatg
    14761 aacttaccgg aaacagccct gtgatcgtgg atctcaagga aatgataagc attgttgcgc
    14821 cgacgaatgc ctggatcctg atcatgggtg aaaacggaac aggtaaagaa ctggtggcgc
    14881 gttcgattca tcgtttgagc ctgagagcgg acaaggatat tgttgaggtg aactgcgcgg
    14941 cgattccgga agaactcatc gaaagcgaat tattcgggca cgaaaaaggc gccttcaccg
    15001 gggcgacgga aaagaaacgc gggaaatttg atctggcgca cgaaggcacg atctttctgg
    15061 atgaagttgc cgacatgagt ttgaaggcgc aggccaaaat tttacgcatt ctgcaggaga
    15121 aaaaattcga gcgggtcggc ggcaacagat tgatcgacat ggatgtgcgc gttctggccg
    15181 caaccaataa ggatctggaa gaggaaatga aagcaggccg tttccgtcag gatctttatt
    15241 accgcctgca cgtcattccg ctggttgttc cgccgttacg cgacagaaaa gaagatattc
    15301 cggcgctggc tgaaaggttt atcctggatt ttacccggaa ggaaggtatg gaaccgaaaa
    15361 tactcagtga tgacgccatc gaactgctga tgaaacatga ctggccggga aatgtccgcg
    15421 aattgaaaaa tattattgaa cgaatgatta ttctgacccc ctccaacgac ataaccgcca
    15481 aggatattcc gccgctcaat acgaaagagg caaagagcgt atttcccggt acaccggttg
    15541 ccgccgctga ttctttcagg gacgccaaaa tggattttga acgacagttt attctgaaaa
    15601 aactggaaga aaacgatgga aacatttcca gaaccgcgga agccatcggt atggaacgca
    15661 gcaacctcca taaaaagctc aggagcctca atgtggtcat caagggggac gcttaaacct
    15721 gtttttcgtt gttgatctgg tttttcattt cggaaattga aaacttgttg aagggtataa
    15781 agataacgcc cagcaccacc acaacgatca tggaaagaaa atgataaacg acagcaaacg
    15841 aaagcgcttc cacttttgcc acgttgaaga ggcttaaccc caagacacag gcaaagtgcc
    15901 agttgccgat gaaaccgggc gccgtcggaa tggctatccc cacaatcaga ataatcatta
    15961 aaacaaacga agcgattacc ggaagatgaa atccgaaagc cagaaaaagt acataaatgg
    16021 ccagcacgtc cagcagccag accgtgaccg aaagaaagat cagatataaa agcaggttga
    16081 tattcgtgac aatttgcaac ccgttggtga agttgtacag caactcgtcg attttatcgg
    16141 ccagccggcc cggaagcatc gccagtactt tgttgaccag ttgaagggtt tctttcctgc
    16201 gcaggatcat aaaaagaata aaacagaata tcgccagcgc cagcaaaaag aaaataatgc
    16261 tggaatggat catccatgaa ggcaaatcga tgaaaaagag aagaaggatg gcaatgccca
    16321 ggacggtaaa gctgtccaga actctttcaa tgataatggt acccagcgcg gacgacattt
    16381 ttacagaact tttttgggaa attaaatatg gtcttgccag ttcccccatc cgtgccggga
    16441 tggccgcaat cgccagaaag ccgaccgatg tgaccgagaa aagggagacc tggtcgattt
    16501 tctccagggg ttgcagaata acgccccagc gataggaccg cagatactgc attaaaatga
    16561 tcagaaaaag aaagaggata acgtaggtga gttcaatttt attcagatca ttcaaaacat
    16621 cctgcagact gattcctctg acggacaagt agaccagaag cgcactgatc agaatgccaa
    16681 agccgacttt tttgttcatg aatttacccg gccattatgc tcacaagcca tcattctttg
    16741 accaggttgt ccgcaattct gtcgtgtatc agttgagaag aaagcttgtt tccaataacg
    16801 ccgacgcgaa tcgccaccgt ggttaggttc tttgccttgt acgcaatatc gaatttgact
    16861 ttttcatcgt tgatgatcgt ctgaatggtt ccccgggcga tttctttttc cggtgccacg
    16921 tcaatgccgt gcattccggc gattgttttt tcacaggcct tccatacctg atcgaacgat
    16981 ttattgtaat ccgttttgag ttctccgttg atgtataaat aggtgccggc tcccgcaccg
    17041 gcggccgctc ctccgaccat cagcggtacg catccggaaa aaaggaaagg gatcaatatg
    17101 catgagataa aggatagaaa aacatttctt ttcatgtgtc ttcctcccgt gattcgttgg
    17161 cggtatttaa agcacatgtc gtcggtgatt gcaacggcaa ttatggggaa acagggggtg
    17221 ccgcagaaaa gtcagcgagt gggttgattc agtttttcaa actcgattaa aagttcttcc
    17281 tgctttttac tcagcgttgt gggaatctcc acacacactt cgacgatctg atcgccgcgt
    17341 ccgaacccgc ggatgtgcgg gatgcctttg cccctgagcc tgaaggtttt gccgttttgt
    17401 gttcctctgg gaattttcat tttttccttg ccctgcagcg tgggcacttc gatggtgccc
    17461 ccgagcgccg cctgcacaaa ggagatcggc gcccggcaaa tgacgtcatc tcccgacctg
    17521 ttgaaaaact cgtgctcttc cacgttgatg aaaacataaa gatcgccgtc gggaccgcct
    17581 tgactgcctg tttccccttc tccgcgcaga cgcagacgcg aaccggtttc aacaccggcg
    17641 ggaattttca attgaacggt tttgatgatc cgatcttttc ccgtaccccg gcagttttcg
    17701 cagggggtcg atataacctt gccatgcccg ttgcagtgcg gacaggtgga gctgatggta
    17761 aaaaagccgc tggactgtat cacctggcct cgtcccctgc aggtgcggca gatttccggg
    17821 gaggacccgg gcgccgctcc tgtgccctga caatgatggc atgtggtcag tttttccaga
    17881 tcaattgctg tggtggttcc gaaagcggca tccaaaaaag agatggtcag atcgtaacgc
    17941 aaatcggcgc cggcagtggc gtacgaatga gaccgccccc gagcattgcc gtaaccgaaa
    18001 acgtcatcaa agatattgct gaaatgagaa aaaatatcgt cgaacccgct gaagcccctg
    18061 aatccggtgc tgtttaatcc ttcgtgaccg tagtgatcgt aaatttctct tttttctctg
    18121 tcgcttaata cttcgtaggc ttcggcggct tctttaaatc tttcctccgc ctttttattg
    18181 ccggggtttc tgtccggatg gcactgcatc gctattgcac ggtagctttt ttttatttcg
    18241 tcctcggaag ccgttctcgg gacattcagt atgtcgtaat aatccttctt cgtcatgcgc
    18301 tttttaagtc aacctcgttt tgtattattt ttttaagagc ctcaatttct gtcctgttgt
    18361 ctgttttctg catggcctga agagcacggg agaaatttta ttgttcgatt ggtttttcgc
    18421 cgattctttc ccggtctgag ggttttacgt ccatgtgagg gcttggccat ctgctgaaag
    18481 agcaggacat ctccgttgcc aaacgcgaca tctctgattt tatgcttgcg tgtaatttaa
    18541 caatcaattg gcgttcgtca atattcgaat atcacccgac caatcgtttc agggcctcct
    18601 cgtatttttt tcttgtgttt tctatgattt ccgaagggag ttgcggcgcg ggcggcttct
    18661 ggttccagtt aatggaaagc aggtaatccc ttaagaattg tttatcgtaa cttttttgcg
    18721 catgaccttc tttgtattcg tcggccggcc agaaacgcga agaatcggga gtcagcagct
    18781 cgtcaatcaa cattaagttc ccgtccagga ggccgaattc cattttcgtg tcggcaataa
    18841 tgattcctgc cttcagcgcc atggaggctg ctctctgata gatcgccagg gaaatattta
    18901 ttacgctggc ggtcagttcg gctccgataa tatctgccat ttcttttctg ctgatcggcg
    18961 aatcatgttc tccctccggc gctttggttg tcggcgtaaa aatcgcttcc ggtaatcggg
    19021 aagattcttt caacccggcc ggtaatgtga tgccggagat agtcctgtct ctctgataat
    19081 ccttccagcc cgagccgctg atatagccgc ggacaataca ttccaccgga aggggtgtgg
    19141 ctttttttac cagcatgctt cttccttcca gactgtcgcg gtagggagcc gtctctgccg
    19201 gaaactcggc ggggttggtg gagacaatgt gatgggggat gatatctttc attttttcaa
    19261 accagaaagc ggacatctga ttcagaatca cgcccttgcc ggggacggga tccggcataa
    19321 taacgtcaaa agcggaaatg cgatcggtgg ttacaatcag catataatta ccgacggaat
    19381 aaatatcgcg caccttgccg cgactgatca gattcagttc gtggaagtct gttttctgta
    19441 tgctttttat cataaaaata aattcctttt agcggaaaaa tggattgtat cttttttcat
    19501 gtccgacggt ggaggtcggt gtgctgccgt aattatgtcc gggcatgact ttggtatcat
    19561 cgggtaaggt aaatattttt tccctgacgg ctctggataa ttcctgccac gagccgcccg
    19621 gtaaatccgt tctgccgacg gcttccacaa aaagcgtatc tccggtaaag acaaagccgg
    19681 gcgtgtaaag acagattcct ccgggagaat gccccggtgt atgaatcacc ctgagttcga
    19741 cgttgcccgc ggaaaggatg tctccttcct taaccagcct atcggccggg ggagatgctt
    19801 ttgcgccgaa cattctcaag atcatggccg gtgtatgcgt cagcattatg gcgtcgtcct
    19861 gatggatgat aattttcgcg cccgttgctt tctgcatttc cgtattgccg ctgatgtgat
    19921 ccacatggcc gtgcgtattg acgatataat taatccgcaa attatttttt tgagcttccg
    19981 ccacgatgcc tttaatatta tccgccggat cgatgaccag tgcgtctccc gttatcgggt
    20041 cgcctacaat ataggcaaat accgccatgg aactgacctg catttgttta acgaacatgg
    20101 cttgtctccc tggaaaaaag tgactggcta ataacattat ttgacgtaaa acgtcaatcg
    20161 aattttattc tataaattgt tgaatttatt ggcgtatgag taaggctccg aagaatccat
    20221 ccatgttgtg cctgtgcgga aatgtccgga aaaatccgct gctgtccgtc aacggacgga
    20281 tgatggtcga agaaggctta cctaaagaaa attgattatt tacccttaaa aaatgctcca
    20341 cgatattttc attttcttcc ggaaggaccg agcaggtgca atatatcagc cggcctcctc
    20401 gtttaacggc agcggaggcc tgttgcagaa tctttttttg ttgtgccgcg aaggagagga
    20461 tatcgttttg cattatgcgc catttgattt ccggattgcg gcgcagcgtt cccagtccgg
    20521 agcagggtgc atccaccaga acacaatcga atcgttccct gaaggcatcc ggcagaccgg
    20581 cgcttacatt gccggcctgc gtttcgatga tggtgattcc caatcggatg atttcctttt
    20641 tcagctcagt gattttttcc tggttgcgat caaacgcaag aatgaaacct ttgttttcaa
    20701 gaagggccgc cagatgagag gatttgcctc ccgcgcctgc gcatgcatcg agaatggatt
    20761 catttctttc gggattgacc aggcaggcaa tcaactgcgc cccttcatcc tggatgcgca
    20821 gtaatccttt gtcgaaaaat tctgttttct ggatcggctt cacggcggtg cttaaaatga
    20881 ttccattcgg ggaatagggg gtggtttggg attcgaagcc ggccgccgca agtttttcct
    20941 ttaaatcgtt gcgcgatatt ttcaacgtgt tgacgcgcag cgtcagcggc ggcagtgtgt
    21001 tgtcggcaga gcacagcgcc tttgtctctt gcagaccgaa tgttttgatc catgtctgaa
    21061 tcatccactc cggatgcgaa tgaaaggcgg ccaggtattc gaccggattt ttcttctcgg
    21121 aaggaaagga tagattctga ccgcggcgca gaaagtttct taaaatagcg ttcaccagcg
    21181 aacttttgtc gggattgatt attttggcaa tttcaacccc ttcattgacc acggcaaaat
    21241 ccggcaaccg ctcgctgaat ctgagctgat acagacttac gcgcaaaaca tttttgatgt
    21301 cttcttgcat ttttgcaaag tcgccgcgat acagtttttt caataaccag tccagatgac
    21361 cgcggaaacg cagaacgccg taaacaagat gggtgagaag acgcccgtcg ggggttccgg
    21421 ataaattgta tttgtcaaga caatcgttga tcaatggacc ggcaaaatca tgggcggcat
    21481 ggacctgatt taaaatctcg acggcctgat ggcgaatagt tttcttcatg attcgggatg
    21541 gatacccacc gtgcttgtat tttcgggatg tttcccccgg acgctctgat aaaaatcgcg
    21601 gatcatgctc atatcgtgct ccggatgccc ggtcgggtat atcagcggtc ccgtcccgcc
    21661 ggttttgcgc gcataatcaa taaatgccag ctggatggga acctgcgctt tgagcgcgat
    21721 ataataaaac cctgatttcc aggcatccgt tttgtggcgg gtgccttccg gcgctaaagc
    21781 aaggatgcat ttttcttttt ctttcagcag ggcggccatt tgatcgacga tatggtgacg
    21841 cgctgctctc tcaaccggaa tggctcccgt ccagctcatc aaaaaggaaa aaggaccgcg
    21901 gaataattct ttcttcgcaa tataccgggc gtcgattttc agggcaaatg ccagaagaat
    21961 tccataaaac aaatcccaat tggaggtatg cggcgcgacg atcaggacga actttggctc
    22021 ccgggggatt ttcccggatg ttttccagcc cagcattttc agcagcagca gggagattcc
    22081 ccgcagcaaa gattttacga aaggcgtgtt aaacatggta tggttcataa aagccccagg
    22141 ccttcttcat aaaaaatttt atttcagctc agcgagtttc caatcttcag gtgatagccg
    22201 cgcaggaaat cagcaatggg cattcttttc tttcccgcca gttgaacgtc ttttacgatc
    22261 acatagccgt cggcggttgc gacagacaaa ctgtcggctt cgatcgcgca gatggttccg
    22321 gggggctgct gtacgttttc atgacgggca accgcactga aaattttcag ggggagtccg
    22381 tccatgaatg tgtaggcggc aggcaaaggg ctcagccccc gaatgagatt gacaatgtcc
    22441 gatgcgttgc tggtccagag aatttttccc gtttctcttg tcaaccgcgg tgcgaacgtt
    22501 acgccggagg tttcctgttt ttgccggctg gcggttccgc tgatcacctg gtcaattgtt
    22561 tgaatcagca gcgcagcgcc cagtttcgcg agacgatcgt gaagctcgcc gaaattttct
    22621 tcgcagtcca gttgtgattc ttcctgcaga agaatatcgc cggaatccat tccttcgtcc
    22681 atcagcataa tcgtgacccc ggtttttttt tcgccgttga tgatctgcca gttgatcggc
    22741 gccgcgccgc gataccgggg cagcagggaa gggtggatat tgaggcaacc cagccgcgga
    22801 tattctataa ccgatttggg cagaatctgt ccgaacgccg caaccgtgat catgtccgga
    22861 tggagttgct gcagcaactc cataaaagac ggatctttta ctttctccgg ttgataaacg
    22921 ttcagaccga attcctgagc cagaatcttg accggcgagg caatttcttt cagccctctt
    22981 cctttcgggc ggtcgggctg ggtgacaaca ccggcaatcg gatagcgctg tccatgcagg
    23041 aggcgcaaag ccggaagagc gaatgcgggc gtgcccataa aaagaatgcg agggttggtc
    23101 atcgggaaaa tatcctttat ttttttaatg taaacacata tacaaaatca tcgtgattgc
    23161 tcttcctgtc cgcacatggc gggatggctc aattgcaaat ttcgatatac gttgttttcg
    23221 aaattttagt ctcataatcc ctgcaaggtg tcaaggatta cgaattatta ggggtaagat
    23281 tatcattact cgcactaaac ataccctcgc gaaattaccg cgggatttcc gtttgtttct
    23341 ggcggcgacc tttctgtttg gcttttcgca gagtattgtt gattccacat tcaataattt
    23401 tttgaatgaa acgtatctga tcgataatct gcagcggggc attttggaaa ttccccgcga
    23461 attgccggga cttctggtcg ttgcggtttc cgcgattgta ttttttctgt gttcacggag
    23521 acaggcagcg ctggccaatc tcctgtgtgc cttcggcatt ctactcatcg gattttattc
    23581 gccgaacttt tccgttatgc tgatctggct tttcatcttc agtatcggac agcatttgtt
    23641 tctgccgttg aaccagagca taggcatgga actggcagtc aagggacaaa ccggcaggag
    23701 gctgggacag ttcagtgccg ttgccaatgt ggcaatgatt atcggcagct tctccatctt
    23761 tgtcgggttt caatattttg atctggactt caagacatca tactttgcgg cttttctcgg
    23821 attcagttcc gttgcggttt tgatcttcat gatgaagaaa gacgaaccgg ttcccgtgag
    23881 gatgaaattc aacctgcgca aggaatatac gctttactac tggctgacca ttttgtacgg
    23941 aacacgcaaa caattgtttc tgacctttgc gccctgggtg ctggtaaccg tctttcatca
    24001 gaaaacgcag gtggtggcaa cgctgctgac cgtcggctgt gtgatcggca tatttttcaa
    24061 gcccatgctc ggcaaggcga tcgacaaact gggcgagcga ctgattctcg cgtcagaggc
    24121 ggtcatactc gtttttgtct gcatcggtta cggtttttcg aaaagtattt ttacggagac
    24181 cacggcgctc tacgcagtgt ttgtctgtta cattgtcgat cagatgctgg cggcggtatc
    24241 gatggcgcgg gcgacatatc tgcagaaaat agccgtcaga ccggaagatg tttcgcaaac
    24301 gctgaccatg ggcgtcagta ttgatcatat cttttccatt ggcatagcgg tgctcggcgg
    24361 ttttatatgg ctgaagtttg gctatcagta tttatttttg accggtgcgg tgattgccgt
    24421 gctgaatttt ttttccgcct tgcagatcaa aacaaaaacg gaaaaactga cgacccctta
    24481 atatcaaagt ggtcaagaac attctgagga gatcaacaca tgaagaaaat aaaagtaact
    24541 gtcaatgatt taatgcagca gaattatgtt tattatcttc aggagccggt cgggaaaaac
    24601 tttcatccgc aatttacccc tgatttaacg ccaggaaaaa tgctggaaat gggcgtattc
    24661 ggcggaaaat atatgaccga ttgcaaggat gagtttccga aacgctggtt tactcgcgcg
    24721 aagctttgtc atgaacgaca tgaccccgat ctgaattatt tcggtgtaac cgcttcacag
    24781 cctttatccg tctggcgtga aaagggctgg atctatcatg aagatccccg tggctggttt
    24841 caatggtatt gccggtatta tatgggccgg cgatgttccg atgatgaacg gcagattaaa
    24901 agatggaaag cgatgaagag acacatttct caaatcagga aacattgtca aaaaggcgat
    24961 atcaactgcc gtcccagaca aaggcaggcg ctgctgaact gggcttacga tagcagaaac
    25021 atttagggat cgagttgtaa tcattatttt cacagactaa aacgcacaga ccatttcttt
    25081 tatgagggat atatgacgga aacatggtta cgaataacca atccggacaa tccgccgaat
    25141 gatgctatgc tgagtgcatg gataggcaat aaagcgtaca agtacttgca gcaggttaca
    25201 aaactcattg tggcgctata tcccgatgtc tttgtgccgg aatggatcta tggcgggaaa
    25261 aagcacggct ggtcgctccg ctataagaaa agcaaatcgt tttgcacctt gattccggag
    25321 aagagtcgtt ttgcgctgct cattgttttc ggtgcacaag aacgggcaaa ccttgagacc
    25381 gtcaaagaca gactttcgca acacacccga agggactacg atcgggcggc aacgtatcac
    25441 gacgggaaat ggctgcttct ccccgtcgat aatgatcagg tcgtagaaga tctgaaaatt
    25501 cttctggctg taaaacgcaa gcctctgacg gcggaatgca taatcaaata acctgataaa
    25561 ttatcttgag acacgtggta tacccatgca tggctccttt gcaatcgccc gaatttcttc
    25621 cgttgacaca agtagtccct ttccctatac tcctaacatt actatcttaa aggagtacct
    25681 tccatgacga tcaatattaa aacagaagag catattatgc tcattgaaat cgaccggcct
    25741 caaaaataca atgccatgac gcgcgaaatg tacacgcagc tcgctcgggc ctattacaga
    25801 ctggatcgcg atgatgattt atgggtcggg gtggtttatg ccaacggacc gcattttacc
    25861 tccggtctgg aactgaccga ctggtcggat gtctttggca gcggagcggg tttcccgttg
    25921 agcggagaaa atgaaattga tcctttttat atgatgaatg aagaacgttg ccgaaaaccg
    25981 cttatttatg cggtgcaggg gtattgcttc acatgggggc cggaagtgat gctcaacgcg
    26041 gatattcgcg tggcggcatc cgataccaag tttgccatgc tggaagtgcg ccgcggattt
    26101 ttcccgtgcg gtggcgcaac aatccggtta gccagagata tcggctggtc caacgccatg
    26161 cgctatcttt taaccggcga ccagtggagt gccgaagaag cctatcggat ggggctggtt
    26221 cagtatgtca ccgaaaccgg caagcaactg gaaaaggcga tggagcttgc gcgaaaagta
    26281 gccagtgccg cacccttggg tgtgcgtaac attttgcaaa acgcgcgcct ggcggaaaga
    26341 gaaggagaac aggcggcgaa aaaggttatt tatcaggaag tatccaaagt gatgaagagc
    26401 gaagatatga aggaaggcct gcaatccttc atcgaacgcc gcgcagcggt tttcaaaggg
    26461 aaatgatcca tccgttcaat gatgtgatgc gataacttct gaagaaaatg cgaggaagat
    26521 aaatggatcc gacttttttt aatgccggag tattcaaatt tgttattctg cccctgctga
    26581 ttttttccac gcgtatctgc gatgtgacgc tcgatacgtt acgcattatt tatgtgtcgc
    26641 gtggactaaa attattggca gcgtccatcg gatttattga agttctgatc tggttgtttg
    26701 ctattactca aattttcaag aatctgacaa atcctattta ctatgtcgcg tatgcgggcg
    26761 gttttgcgat gggcaattat gtcgggattt taattgaaga aaaaatggca gttggcactg
    26821 tcgtgatccg aaccattaca cagaaggatg ctggggtatt aattaaatgg ctaaaggcgg
    26881 agggatatgg cgttacatac atagacgccc agggcgcttt tggtcctgta aaagttcttt
    26941 ttacaattgt aaaaagaaaa gatattgatc atgttctggg gatgatccgc acgtgtaatc
    27001 cgttggcatt ttatactatt gaagacattc gttcctccag caagggagta ttcccgattg
    27061 ttaaatccag gaagaacaga tacaaagaac cgtaacagaa gcgctgagaa taaaagtgtt
    27121 taatgtgaag aaatattttc agaaaaagtt agtcgtgaag gatagagtca atggaatgta
    27181 aaaaagaaaa aaatctcgat aattgcaatt gcacctatga gccctgcagc agaaaggggg
    27241 cctgttgtga ctgcctgtct tatcacttga agcagaggca actgcccgcc tgctgctttc
    27301 cgaaggacgc ggaaaaaacc tacaacaggt cttttgaata ttttaccgaa ctggtatcgc
    27361 agcgccggat ataaaaagca tgatgttcct gcaagggaag cacgtgaagt cttatcggga
    27421 ggcaggtatt tatgatgccg ctgacgatgt ttgttgaaat cgccgcaatt ttgagtttca
    27481 caatcgtgat cttcactgga attatgacga taaaataaac gcagccaggg aaaagtaaac
    27541 aatcactgtg cagatatcgg ccagcgccag cgtcaggggt ccggaagcga cgtgaacgtc
    27601 aagttttagt ttgtgcaagg aataagggag gctcacaccc aaaaaacatg caaagagcaa
    27661 cgacgttaaa attcctgatg aaattatcag gccggccgta aaatcgtttt tccatacaac
    27721 aacgataaat ccgacaataa acgcgcaggc caaagccatt aaaaacgtgc gcgtcagttc
    27781 cctttgcatt gtttggaaat accagccgga ttctgcttta ctatgatgca tggaatgaac
    27841 cgttatggcc atggtctgca tggagacgct ttcgcccagg cctaaaacca gcgtcagaaa
    27901 gaaagccaaa accaggcttt gcgccagcgt ggcctcgaaa aaacccacca gcagcgcgca
    27961 aacagtgccg ctggtaatgg tagccaaaag ccaggggaag cgataacgga aaacgctcca
    28021 gggcgacttg tgtcgcactt cggaaatttt aataccaaga gtatcaaaca cgctgtgcac
    28081 ctgtccctgc tcctcgatat caatcatttc ctcggtaaac acgttgatat cgataacccc
    28141 cagcattttt tcatggtcat cagtgattgg aaacgccaga taacggtgaa aaataaaaaa
    28201 ttccagcgct tccactaagg ttgcgctggc ggggattaca acaagatttt ttgacatgat
    28261 ttcttcaatt tttacatcca acggagacgt caggagtctt cttgtctgga ttgttccgac
    28321 caaacgggct ttgccatcaa gaacataaaa ataaagaatg gtggattgtg tctggcagtt
    28381 gcggatacaa tccaatgcct cacccacggt attgtcgcta tagatagaca caaaatctgt
    28441 gctggcgtgt ttcatcacgg ggtcattgag atcttctatt tttatggaat gatccatgcg
    28501 atgcctttcc tgtatagaat cagaataacc tcaatgttga atatatacgg cttttttaac
    28561 taacacaaac catttcattt atcagatatt aatcatgatt aatacggctc atcagaacgc
    28621 tgaagaatga tatagctttt aagtataaaa ttaagaagcg gaaaagtccc tgtgacaatc
    28681 ttctgcgcct tatggaattg catgacaaca gtgacgctat cgtctgagat ggcgaaacgg
    28741 ggttgacgat gctctttccc gatggatgcg gaaaaaactt acaacaggtc ttttgaatat
    28801 tttagccaac cggtatcgca gcaccggatg tagaaaaaag ccgttttcaa atgttatttc
    28861 tttcttgttt ttttcgaaac cgtttcgttc tgttgaaatg ctttgtcata tagaatacgt
    28921 cggacggttt ctctgtgttc agcagccata acgcaggcca tctgatcggc tgccgtgacc
    28981 acttctgaca gggcaacgcc gacttcggct tcaccggccg caaccacggt agcgcctgct
    29041 tgacgcagag cggctgcgtc acggagatga gcgcaacgca ccatgatttg caaatcagga
    29101 ttgagaagac gcgcgttctt gatgatctcg gaagcgtctt caatgtcggt gctgataatc
    29161 aggctgccgg ctgttgcgat gcccgcatct tcgagcgttc ccggacgcag aacatcaccg
    29221 tatatggcct tgtatccctg ggcgttcagt tggcgaaccg atttcaggtt gaggtcgata
    29281 accgtgatct cgttgccgcg ttccgtcaga agattgtgaa cgatttgtcc gatgggaccg
    29341 tagccgatga ggatgcagtg actgggatta actgtttgcg gttgactttt gaccaccgga
    29401 agggctttat ttgtctttga agacaaccgg cgtgcccagc ggtagacata aggattcaac
    29461 gcgatcgaga tgatggaggc ggcgaccagc gcattccatc cggcatcgtt gatgattttg
    29521 agttgaagag cgactgtgcc gagaataaaa ctgaattctc caacctgcga aaaggcggct
    29581 ccgatgggga tagccgcagc cagaggctgg ccaagcatcc gaaccgtcaa agcggcggca
    29641 agcggcttga taataacgac cacaaggagc acagttgcga taacgaaagg cacctgtata
    29701 agactcagcg gatcgaacag catcccgacg gaaacgaaga agagcactgc aaaagcgtcg
    29761 cgcatcggca gggcgtcgat gcccgcccgc gcggcaaatt cggaacgccc cacggccaga
    29821 ccggcaagaa aagcgccaag ggccatcgaa acgccaaaca gttttgcgga accgacggca
    29881 atcccaacgg ccagaaccag cacggcaagc gtaaagagtt cccgggaccg tgttttggcg
    29941 atgcgttcca acgcccatgg aataatccat ttcccgagaa ccacgacaat agccaacagt
    30001 ccgacaatct ttattccggc caatccgaga acggctccaa tgttctgatc catgcttctg
    30061 tccggggaga aaataatggg cagaagaagc aggagtgcaa cggtgagaat gtcttctaca
    30121 acggtccagc cgatggcgat gtgcccgatc ttggcgtgca gatcatgcca ttccgtcagt
    30181 accatggcca tgactaccgt gctggccacc gaaatagcca tgcccaggat gaatccggat
    30241 gcccagtccc agcccatgaa atgcagcaag acagccaggg cggatgtgga caaggtgctt
    30301 tgaatcaacg cgcccgggac ggctaaacgc cataccgcga tcagttcacg cagatgaaaa
    30361 cgtaatccga ttccgaagag cagcaggatc acgccgattt ccgcaaactg ctccgcaatg
    30421 gtccggttga cgacaacacc gggagtgaaa ggaccgacga taatgcctgc cagcagataa
    30481 ccgatgatgg gagataattt cagacggacg gcgatatatc cgaagaaaag agcggcagca
    30541 agtccaacag caaaggttat cagaacgctc aattcatcca tcgcttatcc ctttaagaaa
    30601 aaatgtttga aaaagacatg atgttgttat ttcccgcatt agaggcgtca atgctgtgac
    30661 tgagaaaaga taaaaaaaca ctacaatcta actataataa tttataagaa aaatcaattt
    30721 ttttaagtcg ttatcaaacg gttttcttgt acatacggcg cgcatctccg gaaatgttca
    30781 ttgacatgca tggttatgtt aactatattt gtacaaaaga tggaatttgt tcttctcatg
    30841 cacacgctgc tgcgacgcaa tacacattga tcatgattca ttagatggta tagatcattt
    30901 ctcgattacg ataaaacttt cggaccgcgc agatatccag cgatcccggt cattctttta
    30961 ataaagctct gttgaccgct gaaaaatctg gaaagggcaa taatgaaagg agtgatatat
    31021 gaaacaattc aaaagcattc tctatgtcac gggagaaaac ggcgatcagc aatccgcgat
    31081 tgcgcgcgcc gtgtcactgg ccgaaaacaa tcaggctgat ttaaccgtca tcgatgttat
    31141 tcccacggtg atgggtgaat attccgaaga aatcatgagt agccggatgc agtttttgga
    31201 atccttcacc gaaccttata ataaacggtt ggatattcga atcagtgtgg aaacgggtac
    31261 ggtttttatc gagatcatcc gggaagtgct gcgcaacaaa cataatctgg ttattaagcc
    31321 agcggagaat cccgatttcc tgaaaaggtt attcggaagc acggatattc atttgctgcg
    31381 caaatgtcca tgcccggtct ggattatgaa gttgccggaa aaatcgaatt acagctgtat
    31441 tatggcggct gttggtttta agccgctgga aactccggag acggaagaaa atcttaaccg
    31501 ggaaattctc gatttagcca gttctctggc tctgtccgat tttgcgtctc tgcatgtggt
    31561 tcacgcctgg gaagcatttg ccgcccacta cataagaagc cgcggcgaca aggagcacga
    31621 agccatggcg gtgtatgttg aaaaagagcg ctctctccat cagaacgcac tgaatgttct
    31681 tatggagaaa ctgagcaaac gtgtgggtga tgatgtttac caggagcttt cgccgagtct
    31741 gcaccttctc cagggagatg cacagaaaat gatcgcggcg ttggcagcca agctccaggc
    31801 ggatctcgtc gtgatgggca caattgcccg caccggtatt tccggactca tcatcggcaa
    31861 cactgcggaa gctattcttg accaactgga atgttctgtg cttgcggtta aaccgcccgg
    31921 atttgtgacg ccggtcaaac ttgaagaata atgccggtcc cgtcagcgga tccagaggga
    31981 gggcagattg aaaagacttt catcgaaaag cacatttttt attaaaagaa tattcccccc
    32041 gctgtggttc ggatgcatca tcttttttgc gctgatggca ttgtctgccg agtcgaaagg
    32101 cattcatgta gacgatatct tcatagtatt gcttgccgct attttcatgg tactgatcgg
    32161 ctacttcatc atgaggcatc tggtgtttga tctgatagac gaggcatacg atgaaggggc
    32221 gtcgcttctt tttaaaaata acgggcaatc ggttcgtgtg gatttaaccg ccattaagaa
    32281 cgtcagctat acggtgctca tcaatcctcc aagggtgacg ttgagcctgc gccatgagac
    32341 gcagttcggt gatgaattga ctttctctcc gtccggcagt ttcattccgt tcaagaaaaa
    32401 tgaagagatt ctgggactga ttgaccgcat tgacagggcc agaggcggat cagacaacac
    32461 tttaccgatt caggaggggc aaatatgcaa agaaaattca acgaactgtt gattatcggt
    32521 ttgggcggaa caatattttt cggttcattt tttgccggag aatatctggg cgcctccgaa
    32581 agcaacaaag atagctggtg gacgccgatg accatggctc tttcgctgga tcaaacacgg
    32641 ccggaattcg aactttatct taaaaaagaa ctgctgcaaa agcacatcga aaaaggcact
    32701 ctcctggttg ccaatgacgg ggaaaacctg tcaaaattgg ttttgggtga tataaaaatc
    32761 cggctcaata actggaacaa ggtgaaggcg gaaaaattaa aatatgctgt tataaccgcc
    32821 ttttttttag gagcgagcat tgcgttgctg atcatcggtt tgatgcgatt tcttgctgat
    32881 aaagaagatg cacagtagcg gttgaatcct tatatggaga aacccaaatt tacgataatc
    32941 atttttcaca acaaggcacc tctcaaccaa tgaagctttt gcgctatctg tgggcatcgc
    33001 cgtatactct cctgggactt ttttttgttc cgcttgcgtt attaacgaag ggcaggatga
    33061 aaacggttga cggcgtactc gaaatccatg gaggatttgt tacatggttt ctcaaacacg
    33121 gcttgcctgt ccgaggatat atcggagcgt tgacactggg acatgttgtg ctgggataca
    33181 acgaaaatct tctatctgcg tatcgccgtc acgaacacgc gcatgtcaaa caatacgaaa
    33241 tgcttgggcc gttattcctg ccggtttatc tggcggcatc gatctgggcg tggctcagag
    33301 gcagaggggc atataaagga aattatctgg aaaaaaaggc gaaagagcac agccgtgaat
    33361 tttaaaaaac tcttttaaga tgatgtcgga aagtgtattt cctttcagga atatctgtgc
    33421 ggagaaaata attaaacaat gaaaaaaacg gttaacaagc aaaatctgaa aaccgagttt
    33481 tatatgtcgg aaaaatgtta catcacggaa ttatccaatt ctgccgacga tcctgatgta
    33541 tccattgccc gggcaaggg
//
LOCUS       DAOSME010000024        32926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1758_length_32926_cov_25.756107, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000024 DAOSME010000000
VERSION     DAOSME010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 32926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32926
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_32926_cov_25.756107"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLG98_05320"
     CDS             <1..1157
                     /locus_tag="PLG98_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32707.1"
                     /translation="GTRIVKAFGMETHEIKRFAGENERFFKLNMKAVSVKALSHPLME
                     FLGGLGIAAVIFYGGYNVLAGNSTPGTFFSFLTAVLMLYEPVKRLTNVNNTINQGIAG
                     AERVFSIIDLVPDIQDKPHAAELPPIDRGIEIDNVTFRYEKTPVLKNITLSIRAGEVV
                     AFVGMSGGGKTSLVNLIPRFYDVSEGRVLIDGLDIRDVSLPSLRRQIAIVTQQTILFN
                     DTVRNNIAYGSFEKSDDEIICAAKAANAHDFIMRMPKGYDTNIGELGTKISGGEKQRL
                     SIARAILKNAPILILDEATSSLDTEAEMEVQEALDNLMKGKTTLIIAHRLSTIRNADR
                     IIVLVNGEIVEEGNHETLMQKQGEYFRLYNLQFKDEGSHENNNRLAKDLG"
     gene            1120..2232
                     /gene="lpxK"
                     /locus_tag="PLG98_05325"
     CDS             1120..2232
                     /gene="lpxK"
                     /locus_tag="PLG98_05325"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOE32708.1"
                     /translation="MKTITDWPRIWDNDGSASGYGTIKFIAHLLSFFYLSIVNLRNWL
                     YDHNLLKATRLGCPVISVGNITVGGTGKTPCVVMLARMLQHRGFKPAVISRGYGGRGK
                     GAVNIVSDGQRILLDSEIAGDEPLLMAHALTGIPVITGPRRTATGKTAIDHFGADVLI
                     CDDAMQHRQIFRDVNLVLIDGQDLKDNIHVLPRGRLREPVKALRRASAVVFTRVDGQI
                     AVHQTIENLITKENLPVFQSLHRINDMISADKKICKPFTELEGKRVHAFCGIANPESF
                     KKTLLSTKAVLVAFNIFPDHHRFQEHELEKIKNDFKNSAADYLITTEKDAMRLKNHPE
                     MSKMLFVLRITMEIKDNPQSFENFILHKIRAGTKKG"
     gene            2243..3310
                     /gene="waaF"
                     /locus_tag="PLG98_05330"
     CDS             2243..3310
                     /gene="waaF"
                     /locus_tag="PLG98_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOE32709.1"
                     /translation="MKDEKIKKLPQKGIHKILIRGTNWIGDAILTLPAVASIRATYPQ
                     ARIAMLVKPWVADVYDIFSNLDEVIVYENKYDTATGVFGLARMLREKQFDAAILLQNA
                     IEAAIIALFARIPVRAGYNSDARGLLLTHSVPLTRDIKKVHQVNYYLEMVKALGCVSA
                     NRDMNLETKISREEAQNVLRRYVAPSQKMLIGIAPGATYGAAKKWFPDRFAETLNRLD
                     EQFSVQGIILGGKADRITAEEVRTSARADMINLAGETSLREAIYLISQCRLFISNDSG
                     LMHVAGALNVPTIAIFGSTNPTTTSPVGKQSLIVRKEVSCSPCLKKTCPTDFRCMNLI
                     TVEDVLSVAQTLLKDNRDKDQ"
     gene            3307..3894
                     /locus_tag="PLG98_05335"
     CDS             3307..3894
                     /locus_tag="PLG98_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE32710.1"
                     /translation="MKKNTAVFLDRDGVINEEVGYLDGLEKLKVIPNACKAIQMINQS
                     GMKAIVISNQSGVARGLFTEEFVQTANSHLQKILKTHGASIDDFYYCPHHPSEGKAPY
                     RQICDCRKPAPGMLLRAARDLNINLTLSYLVGDRYIDMIAARRAGVKGILVKTGYGND
                     LLQDDGPDRPTPESEPDFIAADILEAVQWILKDKT"
     gene            3870..4934
                     /gene="waaF"
                     /locus_tag="PLG98_05340"
     CDS             3870..4934
                     /gene="waaF"
                     /locus_tag="PLG98_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOE32711.1"
                     /translation="MDIEGQNVNILIVKLSAIGDVIHTLPSLAELRRLYPDAHITWVV
                     EEAAADLVKHHPCLDEVLISRRKTWIQNVRAGKIRQSLNEGISFIKGLRKRHYDLVID
                     FHGLFKSAIIVFLSRGRRKLGYDSWQELSGLFLNEKIPEDMDKHAVDRYLDFLRHLGA
                     KTPEARFVLPSNDEEEKKAQLLMQKHRLEDKKFIAVNPVALWDTKLWSNEKFARLADL
                     IQNRLKLNVVFTGSDKKPLEAITALMTTAGLHLGGETTLICLAGIYKKARAVVTTDSG
                     PMHLAAAVGTPVIAVFGPTDPARTGPYGSRHTVIRSGEDCSPCFLKTCPEKKCMDHIT
                     PEQVFAAVEKTVMEEKNGVK"
     gene            4921..5091
                     /locus_tag="PLG98_05345"
     CDS             4921..5091
                     /locus_tag="PLG98_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOE32712.1"
                     /translation="MALNKKLLEILICPKCRGKVRFDEKANGIICDKCKLLYEIKDDI
                     PVMLVEEAKNID"
     gene            5383..6630
                     /gene="rho"
                     /locus_tag="PLG98_05350"
     CDS             5383..6630
                     /gene="rho"
                     /locus_tag="PLG98_05350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOE32713.1"
                     /translation="MNIEDIKRLPISELNELGKKLDVPGASGMRRQELIFAILQTQAE
                     QNGVISGSGVLEILPDGFGFLRAIDYNYLPSPDDIYISPSQIRRFNLKTGDTVSGEVR
                     PPKEGEKYFALLKVDTVNFESPEQARDKILFDNLTPLYPQEKLNLEYDPKNYSTRTLD
                     LFAPIGKGQRGLIVSPPRTGKTVLLQDIAHSIAANHPEVVLMVLLIDERPEEVTDMQR
                     SVSGEVISSTFDEPATCHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKG
                     TGNMELHLDRRISDRRIFPAFDLIRSGTRKEELLIPKNNLNRVWILRRLLQEMNPVDA
                     MEFIVGKIKKTENNQEFLDSMNS"
     gene            6766..6999
                     /gene="rpmE"
                     /locus_tag="PLG98_05355"
     CDS             6766..6999
                     /gene="rpmE"
                     /locus_tag="PLG98_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOE32714.1"
                     /translation="MKEGIHPDYQETTITCVCGNVIHTRSTKKDIKTEICSNCHPFMT
                     GKQKIVDTAGRVERFKKKYETKVDATTSKKKKS"
     gene            7278..8357
                     /gene="prfA"
                     /locus_tag="PLG98_05360"
     CDS             7278..8357
                     /gene="prfA"
                     /locus_tag="PLG98_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOE32715.1"
                     /translation="MDIIRDKLHDIELRYRELEGQLSDPKIVSKPGMYQKYAKEYADL
                     KELVETIREYNTLRKQIDEYQDVMTEGDEELKAIVKEELPQLKQQQTDLEDRLTILLL
                     PKDPNDNKNVFLEIRAGTGGDEAGLFAADLFRMYARYAEAQGWRVEVVSSSPAGGMGG
                     YKEIIAQIEGDGAYSRLKYESGVHRVQRVPVTEAQGRIHTSAVTVAIMPEAEEVEINI
                     DPNDLRIDVYRSTGHGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKNKAMKVLR
                     ARLLDAEVQRQNAEQAQTRKSQVGSGDRSERIRTYNFPQGRITDHRINLTRYDLDNFI
                     NGNIGAMIDSLAAHYQAEALNKTKN"
     gene            complement(8392..8886)
                     /locus_tag="PLG98_05365"
     CDS             complement(8392..8886)
                     /locus_tag="PLG98_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32716.1"
                     /translation="MKPGASLTERFDGWFVKPIEKLKELPEGDGGFLALSAALFLCER
                     YYRALTDTLYGKRDDETFKVAAAKDLGLSPEDFNSFWIVYRNGVQHQGTPRHYIDKKN
                     QIKYFFHISDEFGGIPEIFKINAYKREIRLNVWKFADLIVSKFKTNPQVFEKAVSRTF
                     PAVK"
     gene            9057..9917
                     /gene="prmC"
                     /locus_tag="PLG98_05370"
     CDS             9057..9917
                     /gene="prmC"
                     /locus_tag="PLG98_05370"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOE32717.1"
                     /translation="MTFRDILNEATRQLEAADISSARLDAEVLLAFCLQCDRLEFLKN
                     PDGMVGEKSLAAFQNCLSRRLRREPVAYITGRKAFWTFFLEVNQDVLIPRPDTEIIVE
                     ETLDICRKTETSILHILDVGTGSGAIALALAKELPQAKITATDFSAAALAVARKNALA
                     LKLADRIDFHPGNLFEPASGVFDIIVCNPPYISEDEYKELPAGVKNYEPAGALLAGKK
                     GTEFYEKLIRQAPDYLQKNGWLLLEIGAKQEEAVRRLLDKSGFYQDVAMRRDYAGLPR
                     VMRARRKTNG"
     gene            9910..11160
                     /gene="murA"
                     /locus_tag="PLG98_05375"
     CDS             9910..11160
                     /gene="murA"
                     /locus_tag="PLG98_05375"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOE32718.1"
                     /translation="MDKIVINGGIPLHGNVQISGAKNAALPVLASSLLTAGTNTFHNI
                     PDLMDIKTIKILLTSMGAEMEGGKTVRIRVDKINNPVASYDLVKTMRASILVLGPLVA
                     RAGVARVSLPGGCAIGARPVNLHLKALEDMGAHVELKNGYIEAKAKKLKGAEIYFDLP
                     TVTGTENIMMAATLAQGTTVLNNAAREPEVVNLADVLKGMGAKITGAGSDVITIEGVS
                     SLKPTEASIIPDRIEAGTFMIAAGMTRGEINVLGCNPHHLEALINKLRDTGMKISPIK
                     NGLTVSCGKKINSVDVTTLPYPGFPTDLQAQIMAYMAVGSGLSVITETVFENRFMHVS
                     ELLRMGADIVIQGANAVVRGVPKLRGAQTMATDLRASASLVLAALVAEGTTEISRVYH
                     IDRGYETIEKKFSALGADIKRVKS"
     gene            11157..12443
                     /gene="hisD"
                     /locus_tag="PLG98_05380"
     CDS             11157..12443
                     /gene="hisD"
                     /locus_tag="PLG98_05380"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOE32719.1"
                     /translation="MKMIKSSAAGFEKLFQKLRFREGAHSPELLAAVAKIVDDVAASG
                     DRALLKYMKKFDGYAPAAAELEATPAEKKKALAEVKPQEISIIKSAARRIERYHRCQV
                     AHGYKIRPETGVELEQRILPLHRVGIYAPGGKASYPSTILMAAIPARIAGAEEIVLVS
                     PAPAGKLNPYIAAAAEISGVHRIFKIGGAQAVAALAYGTETIPQVDKIVGPGNAYVAA
                     AKKMVFGQVDIDMIAGPSEVVVIADAKANASFAAADMLAQIEHDEMAAALLLTPSETF
                     AITVYREIRRQIKTSARKKIIEKSLDRYCAIFITGSLDEAVNIANRFAPEHLELMTQS
                     PDKILKKVRNAGSVFLGSYTPEALGDYLAGTNHILPTGGTARFSSPLGVYDFYKRMSV
                     LSFSRKAFEKLSRPTADFARMEGLDAHAHSVLIRGK"
     gene            12835..13614
                     /gene="ubiE"
                     /locus_tag="PLG98_05385"
     CDS             12835..13614
                     /gene="ubiE"
                     /locus_tag="PLG98_05385"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOE32720.1"
                     /translation="MISKNNKYPSVKKVTDEERIGIVRDIFSTITGSYDFLNHFLSLG
                     RDIAWRKFAAKKMVFPRSGKFLDIACGTGDLSIIACAAHQDMSVTGADFVHPMVRLAN
                     AKVRKKRLSGRIKFIQANALNLPFADNTFNVTAMAFGIRNIPQKDRAIKEMLRVTVPG
                     GQIMILEMTFIQNRLFKAAYYLYLNFLLPLLAKLFSKNAAAYHYLADSIMNFPKPEAF
                     AAFMEKTGVTDVQIFPRTFGITCLYLGEKPAQNNRATQNHH"
     gene            complement(13625..16282)
                     /gene="polA"
                     /locus_tag="PLG98_05390"
     CDS             complement(13625..16282)
                     /gene="polA"
                     /locus_tag="PLG98_05390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOE32721.1"
                     /translation="MVESNRKPLLALIDGSNYIYRAFYGLPLLTNSKGFPTNAIYGFT
                     NMLLKLKRELKPDYIVVTFDLKGPTARHEEFEDYKATRKPMPDDLVPQVPLIKDVVRG
                     FSICVLEKQGTEADDLIGTLTIRAGDKGWRTVIISGDKDLLQLVNENVTMIDTMKDKT
                     YDADAVKEKFGVGPDKVVEMLGLMGDTSDNIPGVPGIGPKTAQRLIEEYGTVENVIKN
                     AENLKNVKLRESFRKHAEQARLSRQLANIRKDIPIDFDFQDAVVREPDREALSKLFTE
                     FEFSSLLQEIRTDQIKPVLECKMVVNEKALAEFAACLKESHEFSMEFIYEKSQPAHLI
                     GMAVSTGRHVFYVPFGHTNVSGQLTVKDVLSAMAPAFNHTGIKKYFYDLKTALVCLDT
                     LAVCSGDDLQLAAYLLNPAKSSYELDDIAWEYLHEKIPSLNEIAGGKGKMYPLSLSPL
                     DKMTAAAGQRAEAILSLTGVLAGQLEEIDVMDLYRKVEMPLLHVLAEMEKKGVLLDLA
                     LLKQMSAELTQLLALSESKIHHLAGEKFNINSPKQLQHILFEKLNLPAGKKTKEGYST
                     DVDVLTELARSHELPAEVLAYRSLAKLKSTYVDALPALVNPQTGRIHTSYNQTVTATG
                     RLSSSNPNLQNIPIRTLEGKRIRQAFIADPDCFLISADYSQIELRVLAHLSEDDALID
                     AFTSDEDIHSRTASDVFGVFPQMVNPDMRRQAKVINFGILYGMSAFGLAKELGVSMKT
                     AQEYIDGYFNRYKKVRLYLDGILEGARQNGFVSTLLNRRRYLPELKSKIPSVRQFAER
                     MAINAPIQGTAADLIKVAMVNIAKLLLKKKLSARLIMQVHDELVIEAPAGEKADVMAL
                     VKKEMEEVIRLKVPLKVEIASGKNWDEAH"
     gene            complement(16308..16754)
                     /locus_tag="PLG98_05395"
     CDS             complement(16308..16754)
                     /locus_tag="PLG98_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOE32722.1"
                     /translation="MDAIDKKILNILQKEFPLEEQPFLIVGKRCGISEEETIRRVQNM
                     KNEGIIRRIGAVFDGAQLGRVSTLCAARVPEEKIDTFVKVVNASKNVTHNYLRNHEYN
                     IWFTVSAANAEELNIFLNEIKGETGITDLLDMRSVRTFKINASFDL"
     gene            complement(16777..17835)
                     /gene="ahbD"
                     /locus_tag="PLG98_05400"
     CDS             complement(16777..17835)
                     /gene="ahbD"
                     /locus_tag="PLG98_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HOE32723.1"
                     /translation="MSIVLDNKVRMIAWEVTRSCNLNCVHCRAASSLGPYSGELSTEK
                     CLQLVDQIAAVSTPVIILTGGEPLLRPDIFEIAAYGTNKGLRMVMATNGMLVTPAVAQ
                     KMTESGIQRVSISIDGKDAHSHDAFRQQKGAFNGAMQGIEALKNAKMEFQINTTITTN
                     NLDQIKDIFKLAKDIGAAAHHIFLLVPTGRGKDLAAQAITAADYEETLMWFHQESLSC
                     GIQLKATCAPHYFRIMHQNKPKEEKPVKKSGGHFHESTRGCLGGITFCFISHTGQVQP
                     CGYLELDCGNVKKKSFASIWENSEVFRNLRDYSKYEGKCGRCEFIKVCGGCRARAYEA
                     TGNYLAEEPLCLYQPKLK"
     gene            complement(17847..18821)
                     /gene="hemB"
                     /locus_tag="PLG98_05405"
     CDS             complement(17847..18821)
                     /gene="hemB"
                     /locus_tag="PLG98_05405"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOE32724.1"
                     /translation="MQFPEYRARRLRKNENVRRLIRETKLSVDDLVYPLFAVPGKNFK
                     KPISSMPGQFQLSVDHIVKEAKKVQELGIPAILLFGIPAKKDTMATGAFAKDGIIQQA
                     VKRIKNDVPDILVITDVCLCEYTSHGHCGMLEKDSVQNDETLEVLAETALSHARAGAD
                     MVAPSAMMDGQVAVIREALDENAFEDVPIMAYSAKYASSFYGPFREAAESTPQFGDRK
                     AYQMDPANADEAIREISLDVNEGADIIMVKPALAYLDIICRAKQEFDLPLAAYNVSGE
                     YAMIKAAAQLGWLDEERAMLESLTAIKRAGADIIITYFAPDVAKLLSK"
     gene            complement(18827..20014)
                     /gene="ahbC"
                     /locus_tag="PLG98_05410"
     CDS             complement(18827..20014)
                     /gene="ahbC"
                     /locus_tag="PLG98_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HOE32725.1"
                     /translation="MIGISKLYCGAVEPSDVLRYNRKSGDLPSHLLQFSADKKPVVVW
                     NMTRRCNLKCIHCYSNSADIDYPDELTTEEGKKLIDDLAAFGSPVILFSGGEPLIRKD
                     LLELAQYATKKGMRAVISTNGTLITKDIAAKLQKVGLSYVGVSLDGLEKTHDRFRGKK
                     GAFAAAMDGIRNCREAGIKVGIRFTVNKHNLADVKDMFDLLRKEKIERLCFYHLVYTG
                     RGSKLRDEDLNHEQTRKLIDLIAAETKKMFDDGLSPEILTVDNHADGPYLYLKMKKED
                     PERAGEILELLEMNEGNSSGLGIGCVSWDGEVYPDQFWRNQSLGNVRNRPFSQIWTDE
                     KNELLMKLKEKKKHVHGRCAGCLWLNVCAGNFRARAESEGDIWGPDPACYLTDEEIHK
                     KED"
     gene            complement(20011..21564)
                     /gene="cobA"
                     /locus_tag="PLG98_05415"
     CDS             complement(20011..21564)
                     /gene="cobA"
                     /locus_tag="PLG98_05415"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOE32726.1"
                     /translation="MKKNKKGKVYIIGAGPGDAGLMTLKGIDCLREADVVIYDYLVGK
                     DLLKYARSDARFIYAGKQGGAHTLSQWQINDLLVKEANAGNIVARLKGGDPFIFGRGG
                     EEAEKLAANKIPFEIVPGVTSAIAVPAYAGIPLTHRGLTSTVAFVTGHEDPTKGKSDI
                     DWQALARIGTLVFLMGVKNIEKIVKELEDNGRSPKAPAALIRWGTTARQEILTGTLSS
                     IVKLAREQKFAPPAIFVVGEVVALRDTLQWFDSKPIFGKGVVITRPEKQADDLAQLLI
                     KAGANPIHFPTIKIVPPASWREMDNAIKKLEDYDWLIFTSANGVAYFFERLSAKGKEI
                     RDLKGIKICCIGPATARQVQDKGIRVDLVPKEFIAEGILKSFSRTNLKGKKILIARAA
                     KARDVLPEGLKKLGAQVDVVTAYETVGSGKKKKELEELFKENQVDVIAFTSSSTVHNF
                     IKIMGGGFKLPKGVKIACIGPVTAAAARKAGFPVDIHQEEYTIEGLVDALANYFKKKP
                     AAGKRGKGK"
     gene            complement(21664..22581)
                     /gene="hemC"
                     /locus_tag="PLG98_05420"
     CDS             complement(21664..22581)
                     /gene="hemC"
                     /locus_tag="PLG98_05420"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOE32727.1"
                     /translation="MTNIKIGTRGSKLALTQTNFVADKLKKAIPDAEVEICVIKTSGD
                     IMQDVSLLTIGGQGVFVKEIEEALLSEKIDLAVHSMKDVPGENPEGLIFSAILPREDV
                     RDVLVSRNNIKLEFMPKGAKIGTGSQRRGAQIKALLPDVSIVPLRGNIETRIKKIETE
                     NINGVILAAAGMKRNGLTEKITQFLPVETMLPAVGQGALGLQIRKNDDELAKACAVLN
                     DAATSAEVAAERAFLRTLGGGCRLPIAALGKLDGQMLTLEGMLAAPNGTTVIREKISG
                     TIKDAEEMGKKLAEIVLDKGGRRLMDLMC"
     gene            complement(22617..23876)
                     /gene="hemA"
                     /locus_tag="PLG98_05425"
     CDS             complement(22617..23876)
                     /gene="hemA"
                     /locus_tag="PLG98_05425"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOE32728.1"
                     /translation="MIVLTGLNHKTAPLAVREKIFAGCQEKKDLLPALRSIGGVEEVL
                     YLSTCNRVEILASVAEDGNALKELSGFLARNGGLNQAEAANSFYEYRGEDAVRHIFRV
                     ASSLDSMVMGEAQILGQVKDAYREALAEYATGVVINRLMHCSFRAAKRVRSETGIAVN
                     PVSVSHAAVELAKKIFGTLTGKIILLIGAGEMAELTGTQLIEKGAQSIIVANRSLAQA
                     ELLAEKFHGEAVALDALEKKLIEADIVISSTGAPDFIVTADMLKKIHHRRKNRLLFLI
                     DIAVPRDIDPDASELENVYLYNIDNLQDIVDENINARRKEAIKAEKIIDEELERFINW
                     QKELESVPTIVSLRNKADDIVKAEIEKASGWMNDLKKEDQEKIEILINSIVNKVLHSP
                     VSVMKEESSEFNSRDIVAAVRRLFRLD"
     gene            complement(23879..24709)
                     /gene="ccsA"
                     /locus_tag="PLG98_05430"
     CDS             complement(23879..24709)
                     /gene="ccsA"
                     /locus_tag="PLG98_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOE32729.1"
                     /translation="MSFVNLEMLSFNTALVLCGASTIGYLSSLLVKKVKLAKMSTWVL
                     AAAFAFLTVKFFFVMVNSSWFVNPDSKDFLSLCAWAVCAVYLVLQFKTKTRVLGAFIS
                     PLILLFLIAAAGEEAGKSIRPQNLQSGLTAAHLFLTIFGEVLFVIASCAGLMFIMQNR
                     LLKSKKLSGMSRLLPSLNDLDRINHLCLLWGFPILSVGIIAGVIFAEMSWKTGWLTDP
                     KIIWTFAAWMIYGFLLHQRLAIGWKGYRMALISGITFILLIVSYVGVKICCPTLHNFI
                     "
     gene            complement(24696..25379)
                     /locus_tag="PLG98_05435"
     CDS             complement(24696..25379)
                     /locus_tag="PLG98_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOE32730.1"
                     /translation="MLLAAARRAKGRKLLKYYPVFLDIKDKICVIVGGGEVAFRKAER
                     LLECGAKVSIISPKLVRELAALKEKNLISHIAAEYAGDLIDRAVLIIGATDDEKINAL
                     IAQDARARGIPVNIVDDPQKCDFILPAIAQRGDLVMAIGTGGKSPALARHLREELEEQ
                     YGREYAILLNILGTLRTKMEKNAGIGRDWFDALMAAGILELIKAKDSQKVQDIVKKIT
                     GEEVECEFR"
     gene            complement(25495..27171)
                     /locus_tag="PLG98_05440"
     CDS             complement(25495..27171)
                     /locus_tag="PLG98_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32731.1"
                     /translation="MKKIVVFLSVCVLVFFMAVSGMTAVWPPAGFTESVADQNIAFPQ
                     TKATAVTVFAPLPASCPSHPAACDKLNFLRIRHIHGPDNPSNADRILIAQPGILEGAS
                     AFYNVAANLVTRAYDEKGKFVEFWAIDRRSNCLEDMNGLRLARETGNLRDMVDYYYRN
                     KLYHGQYFQGYFKPLSDDVKWLVEMGMEQTLKDWNEIITRGIPDPTVRQQRVYIGGHS
                     LGGFLTGAYACADFDGDPTTLADAGYNQAAGYFALDSLITSDPLLSVQGGDSATLRYL
                     MSKIPDSAVYLMRKGLFERFIFLSGVITPEIMNLLTGVGYAAMVAPNAETDLISYLPA
                     STSVKNCYRFYHSKNIWDYALVTPSIYKFRYTNQALMALFMDDNAMPINIIQASMGFF
                     AGGTVAEKHFPFGSGGTLAIANNSGTALKKGPLYRWINYDEIEGSAIPNNNDGVPFTS
                     AAKEVTDAKDLARSLGALPMDFVEKYFPMRLAVESMMGSDVIVHPDGISQRPVINIVA
                     GDGAKLGLDQLSPDSPIIPGYNHLDVLTAAAVQNDGEPEKVTTHLLDFIF"
     gene            complement(27454..28620)
                     /locus_tag="PLG98_05445"
     CDS             complement(27454..28620)
                     /locus_tag="PLG98_05445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE32732.1"
                     /translation="MSLNINGISRKFSKLAYSLSELAENFSLIPNNDPKQALRIKRFF
                     ISIFVYLLNMPLSYLAYTGGLMEFKELVWGWSITTILMIILYIVFRSGLNKRMKDPSL
                     TFAQISVASLVVMYGLFFVPEAKGILFAVYVLILLFGTFRLDTRQFLKASAFILGTYA
                     VDICLLQIYRPEAIHLKLEVLYWFALGVVLASVSFIGGNISALRRDLAVSHKQLQASV
                     EKIQAMAIHDDLTGFYNRRHLMELLESEHNRSVRTGAVFSLAMIDLDKFKNINDKFGH
                     QAGDDVLMTFSNVIRNVLRKTDFCGRYGGEEFLIVLTQTDLEEARIFAERIRACIEDC
                     LVFDLEPDRQLKVTVSIGLAEHLKNEGIDRTIFRADEAMYKAKKNGRNRVEYSE"
     assembly_gap    29045..29144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29146..30249)
                     /locus_tag="PLG98_05450"
     CDS             complement(29146..30249)
                     /locus_tag="PLG98_05450"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOE32733.1"
                     /translation="MKMKIAVLTGGGDCPGLNGAIKWVTKSALDPRLAANRSVSFDVI
                     GIVEGWKGLIDVDPEDEKSRARHLKHLDEEIVRTWDRYGGTNLGTSRTNPFNPKNDRS
                     ARVLENIKKLGIDAIVAIGGEDTLGVAYKLHKIGIKTVGIPKTIDMDLAGTEYSLGFD
                     TAINIITEEIDRLRTTAGSHGRIFVVETMGRHAGWLALRGGECSGAYIILIPEHPFDM
                     DKVCLLLRERKGREINYSIIVVAEGAKLAGKQEFLKDSHIDDFGHVSVGGAGSFVAAE
                     IEKRTGLEARHISLSHLQRGGTPSAHDRMMARWFGIAAVDMIVKEDFGRMVTLERGDI
                     GTAPLAGVVNKLKLVDVEKYYDTERYNGRRTIF"
     gene            30467..31408
                     /gene="prmA"
                     /locus_tag="PLG98_05455"
     CDS             30467..31408
                     /gene="prmA"
                     /locus_tag="PLG98_05455"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOE32734.1"
                     /translation="MTEKETGKWLKIDISAPPELMEALGNFLTESGVQGVFQESQSSQ
                     AVGSDFPEPVAEDILKAYFPLDSRSDKKLAALQKYIDSLAEIFPDLGKPSFTTETICD
                     PDWGEQWKKYFKPIRVSNNIVVKPTWERYTPSSRDIVIEIDPGMAFGTGQHASTRMCI
                     EAVEDIIMNDRSIKEWKVLDVGCGTGILGITAAKMGAQDVLCADIDRKAVEIAGENAA
                     INDVRHAVRTINKNLAAIDKPRNLIIANLTSNLLLTHRQHLTQLLMPDGYMILSGIIE
                     QDAGKVAQEYAATPLELQRTITEKEWVCYVLKKTSAC"
     gene            31464..32231
                     /locus_tag="PLG98_05460"
     CDS             31464..32231
                     /locus_tag="PLG98_05460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOE32735.1"
                     /translation="MTVPRIYSPESLENKNSCALGEDNVKYLKKVMRLKEGDRIHIFD
                     GFGREYEATIKNFSAKSVLVDLGKAIPSVNRDISITLAQAITKGKKMDTIVKSAAELG
                     ADMIIPFHAVRSVSHIADEKNPRKQTRWQKIAHEAARASHSAYITEVTKILSFPEMLS
                     RCSPKALKLIFWEEESRKTIKDMLTDDTLEACGDFFIVVGPEGGFSKDEIEKTKEAGF
                     FSVSLGRQILKVETAATAIISIIQYEKGIFSSTSQEA"
     gene            32233..32790
                     /locus_tag="PLG98_05465"
     CDS             32233..32790
                     /locus_tag="PLG98_05465"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOE32736.1"
                     /translation="MAFHPYAGFWRRLVAFTIDGTIICFIFIFLFFIASMAFFSGAVS
                     ANGEKFLASLGNAESLSPAMISLWLFYILLNIGYFTYFHGATGRTPGKSLLGMQVVST
                     EGTPISFGTAFLRSVGYLVSNILYLGFIWVAFDRRKQGWHDKIAGTVVIRRESYEQSA
                     GVSIAEPTWSPPKAQQTKPPETNEN"
BASE COUNT         8510 a         8420 c         7822 g         8074 t
ORIGIN      
        1 ccggaacgcg catcgtcaag gccttcggca tggaaacgca cgaaatcaaa agattcgccg
       61 gcgagaacga gcgctttttt aaattgaaca tgaaggccgt ttccgtgaaa gccctctccc
      121 atcccctgat ggagtttctg ggcggactgg gtattgcggc cgtcatcttc tacggcggct
      181 acaacgttct ggccggcaac tccacacccg gcacgttttt ttcctttttg accgctgtgc
      241 tgatgctgta cgaaccggtg aaaagactta ccaacgtcaa caacacgatc aatcagggca
      301 ttgccggtgc ggaaagagta ttcagcatca ttgacctggt tccggatatc caggacaaac
      361 cccacgccgc cgaattgccg ccgattgacc gcggcatcga gatcgataac gtgacattcc
      421 gttatgaaaa aacgccggtt ttgaaaaaca taacgttgtc tatacgggca ggcgaagttg
      481 tggcgttcgt cggcatgagc gggggaggaa aaacgtcgct ggtcaatttg atcccgcgat
      541 tttatgatgt cagcgaaggc cgcgtgctta tcgacggtct ggacattcgc gacgtttccc
      601 tgccgtcgct gcgccggcag attgccatcg tcacgcagca gaccattctt ttcaatgata
      661 cggtgagaaa caatatcgcc tacggcagtt tcgaaaaaag cgacgacgag attatctgcg
      721 ccgcgaaagc cgccaatgcc catgatttca tcatgagaat gcccaaaggg tacgacacca
      781 acattggaga actcggcacg aaaatttccg gcggagagaa acaacgtctt tccatcgccc
      841 gtgccatttt gaaaaacgct cccattctga ttctggacga ggccacctcc tcgctggaca
      901 cggaagcgga aatggaagtt caggaagccc tggataacct catgaaaggc aaaaccacgc
      961 tgattatcgc gcacaggctt tccaccatcc gcaacgccga cagaattatt gtgctggtga
     1021 acggtgaaat tgttgaagag ggcaatcacg aaacgctgat gcagaaacag ggtgaatatt
     1081 tccgactgta caatttacag ttcaaggatg aaggaagtca tgaaaacaat aaccgactgg
     1141 ccaaggattt gggataacga cggaagcgca agcggttacg gcacgataaa atttattgcg
     1201 catcttttat ctttcttcta tctttccatc gtcaatttac gcaactggct ctacgatcac
     1261 aacctgttaa aagcgacccg gctgggctgt ccggtcatca gtgtgggcaa tatcacggtc
     1321 ggcggcacgg ggaaaacacc ctgcgtggtc atgctggcgc gcatgcttca gcaccggggc
     1381 tttaaaccgg ccgtgatcag ccgaggctac ggcggacgcg gaaaaggcgc cgtgaacatc
     1441 gtatcggatg gacagcgaat ccttctggac agcgaaatag ccggagatga accgttgctg
     1501 atggctcatg ctctgacggg cattccggtc attaccggac cccgaagaac agcaaccggc
     1561 aaaaccgcca tcgaccactt cggcgcggat gtgctgatct gcgacgatgc catgcagcac
     1621 cggcagattt ttcgcgatgt caatctggta ttaatagacg gtcaggatct gaaagacaac
     1681 atccatgttc tgccgcgggg cagattgcgg gaaccggtaa aagcactccg gcgcgccagt
     1741 gccgttgttt ttacacgcgt tgacgggcag atcgcggtcc atcaaacgat agaaaacctg
     1801 atcacaaagg aaaaccttcc tgtttttcaa agtcttcacc ggataaacga tatgatcagc
     1861 gcggataaaa aaatttgtaa accgtttact gagctggaag gaaaaagggt ccacgcgttt
     1921 tgcggaatag ccaatccgga gtcgtttaaa aagacgctgc tgtcaaccaa agccgttttg
     1981 gttgcgttta atatttttcc ggatcatcac cgttttcagg aacacgaact ggaaaaaatc
     2041 aaaaacgatt ttaaaaacag cgcggcggac tatctgatca cgacagaaaa ggatgcgatg
     2101 cgtttaaaaa atcatccgga aatgtcaaaa atgcttttcg tcctgcgcat aacaatggag
     2161 atcaaagaca atccgcagtc gtttgaaaat tttatcttac acaagatccg ggccgggacg
     2221 aaaaagggat gaagggggtt ctttgaaaga cgaaaaaatc aaaaaactgc cccaaaaagg
     2281 catccataaa attctgattc gcggcaccaa ctggatcgga gatgcaatac tgaccttgcc
     2341 tgccgtagcc tccatacgcg ccacttatcc gcaggcccgg attgccatgc tcgtcaagcc
     2401 gtgggtggcg gatgtttatg acattttttc caacctcgat gaagtcatcg tctatgaaaa
     2461 caaatatgac acggccaccg gcgtattcgg gctcgcccgg atgctccggg aaaagcaatt
     2521 tgacgcagcc atcctcctgc agaatgccat agaggctgcc atcatcgcgc tttttgcaag
     2581 gattcccgtc cgggcaggtt acaattcgga tgcccgcggc cttttgctga cgcacagcgt
     2641 gccccttacc cgcgacataa aaaaggtcca ccaggtgaac tattatctgg aaatggttaa
     2701 ggcattgggc tgcgtttccg cgaaccggga catgaatctg gaaacaaaaa tcagccgcga
     2761 ggaggcacaa aacgttttgc gccgctatgt cgcaccgtcg cagaaaatgc tgatcggaat
     2821 agcaccgggc gccacctatg gcgctgccaa aaaatggttc ccggaccgtt ttgcggaaac
     2881 gctcaaccgg ctggatgaac aattttctgt gcagggaatt attctgggag ggaaagctga
     2941 ccgaataacg gccgaggagg tccggacatc ggcccgcgcc gatatgatca atctggccgg
     3001 cgaaacctct ttgcgggagg cgatttatct gatttctcaa tgccgtcttt ttatcagcaa
     3061 tgattcggga ttaatgcatg tggcaggcgc gctcaatgtt ccaacgatcg cgatattcgg
     3121 gtcaaccaat ccgacaacca cgtcgcctgt gggaaagcaa tccttgatcg tccgcaagga
     3181 agtttcctgc agcccctgcc tgaaaaaaac ctgtccgacg gatttccgct gcatgaactt
     3241 gattaccgtt gaggatgtcc tgtcggtcgc tcaaacattg ctgaaggata accgggataa
     3301 agatcaatga aaaagaatac ggcggttttt ctggacagag acggggttat caatgaagag
     3361 gtcggttacc tggacggcct tgaaaaactc aaagtgattc cgaacgcctg taaggcaatt
     3421 cagatgatta atcaaagcgg catgaaagcc atcgttattt caaatcaatc cggcgtcgcc
     3481 cgaggattgt tcacggaaga attcgtgcaa accgccaaca gccatttaca gaaaattctc
     3541 aaaacacacg gagcctctat tgatgatttt tattactgtc cccatcatcc ctccgaaggc
     3601 aaagcgcctt accgccaaat ttgcgattgc cgcaaaccgg ctccgggaat gctgctgcgc
     3661 gcggcgcggg atttaaatat caatcttacc ctttcttatc tggtgggcga ccgatacatc
     3721 gatatgatcg ccgccagacg agccggggtg aaaggtattc tggtgaaaac aggctacgga
     3781 aacgacttgc tccaggacga cggaccggac aggccaaccc cggagagcga acctgatttt
     3841 attgccgccg acattctcga ggcggtacag tggatattga aggacaaaac gtgaacatcc
     3901 tgattgtaaa attaagcgca ataggcgatg tgattcacac gcttccgtct ctggccgagc
     3961 tgcgccggct ctatcccgac gcccatatca cctgggtggt ggaagaagcg gccgccgatc
     4021 tggtcaaaca tcacccctgt ctcgacgagg tgttgatttc acgccgcaag acttggatac
     4081 aaaatgtccg ggccgggaaa atcagacagt ccctcaacga aggcatctcc tttatcaaag
     4141 gcctgcgaaa gcgccattat gatctggtca ttgattttca cgggcttttt aaaagcgcaa
     4201 tcattgtctt tctcagcaga ggcagaagga aactgggata cgattcctgg caggaactca
     4261 gcggtttgtt tcttaatgaa aagattcccg aagacatgga taaacacgct gttgaccgct
     4321 atctggattt tttgcggcac cttggcgcaa aaacgccgga ggccaggttc gttcttccgt
     4381 caaacgacga ggaagaaaaa aaagcacaac tgctcatgca gaaacaccgc ctggaagaca
     4441 aaaaattcat cgcggtcaac ccggttgccc tatgggatac aaaactctgg agcaatgaaa
     4501 aattcgcccg gttggccgac ctgattcaaa accggctgaa attgaacgtg gtgtttaccg
     4561 gcagcgacaa aaagccgctg gaagcaataa ccgctttaat gaccaccgca ggcctgcacc
     4621 tgggtgggga aacaaccctg atttgtcttg ccggtattta taagaaagcg cgagcggtcg
     4681 tcaccacaga ttccggccct atgcacctgg ccgccgcagt cggaacaccg gtaattgctg
     4741 ttttcggacc gacggaccct gcccggacgg ggccctatgg atcaagacac acggtcatcc
     4801 gatccggaga agattgcagc ccgtgttttt tgaagacatg tccggaaaaa aaatgtatgg
     4861 accatataac acctgaacag gtttttgcag ccgtagaaaa aacggtcatg gaggaaaaaa
     4921 atggcgttaa ataaaaaact gctggaaatt cttatctgcc ccaaatgccg cggcaaggtt
     4981 cgctttgacg aaaaagccaa tggcattatc tgcgataagt gcaaattgct gtatgaaatc
     5041 aaggacgata ttccggtcat gcttgtggaa gaagcaaaaa acattgactg aggcggatgt
     5101 tttttccttg acggttgtga tattgaaggc ctaaaataaa ttttcttgac agcgaaatta
     5161 taatcatata agtttattgg caacgagaaa aggctattta gccctaccct ttggagcgtt
     5221 tcaagtattc aaatacacaa gccgaaacta aatccgtaat tactaaaaat cttagctatc
     5281 tttattttgt ttctttaaac acaatgcagt aaagccgaga caaatatcca atatcagggt
     5341 ttctttattt aacccgattc caaattaagg caaggaacta ttatgaacat cgaagacatt
     5401 aagcgattgc ccatcagcga gttgaatgaa ctgggcaaaa aactggacgt gccgggagcg
     5461 agcgggatgc gtcgtcagga gctgattttt gccatcctgc agacgcaggc ggaacaaaac
     5521 ggcgttattt caggctccgg cgttctggaa attttgccgg atggattcgg ctttttacga
     5581 gccatcgatt acaactacct gcccagtcct gacgatatct atatttctcc gtcacaaatc
     5641 aggagattta atttaaaaac cggagatacg gtttcgggcg aagtccgtcc tccgaaagaa
     5701 ggcgagaaat atttcgccct tttaaaagtc gatacggtaa attttgaaag tccggaacag
     5761 gcccgtgata aaatcctttt tgataacctt acccctctgt atccgcagga aaaattaaat
     5821 ctggaatatg atccgaagaa ttactcaacc agaacactgg atctgttcgc gcccattggc
     5881 aaaggacagc gaggattgat tgtttctcca ccgcgaaccg gtaaaaccgt tctgcttcag
     5941 gatattgccc acagtattgc cgccaatcat ccggaagtcg ttctaatggt tctcctgatc
     6001 gatgaacgtc cggaagaagt tacggatatg cagcgcagcg tttccggcga agtcatctct
     6061 tccacctttg atgagccggc gacctgtcac gtgcaggtag cggaaatggt tatcgaaaaa
     6121 gccaaacgtc tggtcgaaca taaaaaagac gtggtgatat tgctcgacag tattacccgt
     6181 ctggcgcgcg cctataacac ggttgttccg cccagcggca aggttctttc cggtggcgtg
     6241 gattccaatg ccttgcataa acccaaacgc ttcttcggcg cagcccggaa tattgaaaac
     6301 ggcggcagcc tgaccattat ttccacggca ttgattgata ccggaagccg gatggatgaa
     6361 gtcatttttg aagaattcaa gggcacgggc aacatggaac ttcatttgga tcgccgtatt
     6421 tccgaccgca ggatcttccc cgccttcgac ctgatccgtt ccggaaccag aaaagaagaa
     6481 ttgctgatcc ccaaaaacaa tctcaatcgc gtatggattc tgcgccggct gttgcaggaa
     6541 atgaatcccg tcgacgccat ggaattcatt gtcggcaaaa tcaagaaaac ggaaaacaat
     6601 caggaatttc tggattcaat gaattcgtag ccgcataaaa atgttttcta tttttacttg
     6661 aatttttgtt ccatatattg taaggcaggg caacgtttgg ccaagaagac aaaaaacact
     6721 aatgattaat tatagataaa aaatcaggga ggaaaaataa gtatcatgaa agagggcatt
     6781 catcctgact atcaggaaac aacaattacc tgtgtttgcg gtaatgttat ccatacaaga
     6841 tccaccaaaa aggacatcaa aacggaaata tgttccaatt gccatccctt tatgaccggc
     6901 aagcagaaga ttgtcgacac agccggccgt gtggaaagat tcaagaaaaa atacgaaacc
     6961 aaagttgacg caacgacgtc caagaagaaa aaatcttgat aaaataacat cttatcagag
     7021 aagaaccttc atgtcgcaac atccacgaat gaagaaaaag caatttccct gcacgctgct
     7081 cgatccggaa ttttgccttg atcatgccaa gaaacatgtt tgcctgattt tgaacaactt
     7141 tcgttatctt tcagaaacgt tgttaaacgc ctccctgcat cgccatttat aggtttcctt
     7201 caaaactcag gaaggaagcc tgtcaaacgt aacccaaaac gccttaatca ttacatttat
     7261 tattgaagac ataaaacatg gatattatac gggacaaatt gcacgatatc gaactgcgct
     7321 accgggaact ggagggacaa ctcagcgatc ccaaaatcgt ttccaagccg ggaatgtatc
     7381 agaaatatgc caaagagtat gccgatctga aagagctggt ggaaacgatc cgcgaatata
     7441 ataccctcag aaaacagata gacgagtacc aggatgttat gacggaaggt gacgaagaac
     7501 tcaaagcgat cgtcaaggaa gaattaccgc aactcaagca acaacaaacc gaccttgaag
     7561 acagattaac catcctgctt ttacccaaag atcccaacga caataaaaat gtctttctgg
     7621 aaatcagggc cggaaccgga ggtgacgaag cgggtctttt tgccgcagac ctgttccgga
     7681 tgtacgcccg ctatgccgaa gcccagggct ggcgcgtcga agtggtaagc agctctcctg
     7741 cgggcggcat gggcggatac aaggaaatta tcgcacagat cgaaggcgac ggcgcttaca
     7801 gccgactgaa atacgaaagt ggcgttcatc gcgtccagcg cgttccggta acggaagccc
     7861 agggaagaat tcacacctcc gccgtcaccg tggccatcat gcccgaggcg gaagaggtcg
     7921 agatcaatat cgatcccaac gatctgcgaa ttgacgttta ccgctccacc ggtcatggcg
     7981 gccagagcgt caacacgacg gattccgccg tccgcatcac ccatctgcca accggactgg
     8041 tcgtgacctg tcaggatgaa aaatcccagc tcaagaacaa gaacaaggct atgaaggttt
     8101 tacgggcgag acttttggat gccgaggtgc agagacagaa tgccgaacag gcgcagacac
     8161 gcaaaagcca ggtcggcagc ggcgaccgct ccgaacgcat ccgcacctat aatttccctc
     8221 agggaaggat taccgatcac cgcatcaatc tgacccgcta cgatctggat aatttcatca
     8281 acggcaatat cggcgccatg atcgactcgc tggcggcgca ttatcaggcc gaagctctga
     8341 ataaaacgaa aaactaaaaa agcatcacct tgatttttga gtggttccga tttatttcac
     8401 tgcaggaaac gtacgggaga ccgctttttc aaagacttgc ggattcgttt taaacttgct
     8461 gacgatcagg tcggcaaact tccagacgtt cagccggatt tcccgcttat aagcgttgat
     8521 tttaaaaatt tccggaatcc cgccgaattc gtcggagatg tgaaagaaat atttgatttg
     8581 atttttcttg tcgatgtaat gtctgggcgt tccctgatgc tgcacgccgt ttctgtatac
     8641 aatccagaag ctgttgaaat cttccggact cagccccaga tccttggcag cggcaacttt
     8701 aaatgtttca tcatctcttt tgccgtaaag cgtgtctgtt aaagcgcggt agtatctttc
     8761 gcagaggaac aacgcggcgg acagggccag aaagcctccg tcaccctccg gcagctcttt
     8821 caatttttct atcggcttca caaaccagcc gtcgaatctt tcggtcagac ttgcacccgg
     8881 tttcataaac atcctcgcca aacagcatga tgcttctgtg gctgtttgag gtcaattctt
     8941 ttaaaatatt aatggcttgg atttataaac acttgacatt tattttgcaa cactttattt
     9001 ttttcaaaaa tcatggggtt gattccatta tcgcaccgta ttctgcagga gacatcatga
     9061 ctttccgcga cattctgaat gaagcaaccc ggcaactgga agcggcggac atttcttccg
     9121 cacgtcttga tgcggaggtc ctgctggctt tctgcctgca gtgcgatcgc ctggaatttt
     9181 taaaaaaccc ggatgggatg gtcggcgaga aatcgctggc agcgtttcaa aattgtctat
     9241 ccagaagatt gcgccgggaa ccggtggcct acattacagg ccggaaagcc ttctggacat
     9301 tttttctgga agtcaatcag gatgttctga ttccgcgtcc ggacacggaa atcatcgttg
     9361 aagaaacact ggacatctgc cgcaaaacgg agacgtctat attacatatt ctggacgtcg
     9421 gcacaggcag cggcgccatc gcccttgcgc tggcaaaaga actcccgcag gccaaaatta
     9481 ccgcgacgga tttttccgcc gccgcgttgg ccgtggcccg gaaaaacgcc ctggctctga
     9541 agctagcaga tagaattgat tttcatccgg gaaatttatt tgaaccggca agcggcgttt
     9601 tcgatatcat tgtctgtaat cctccgtata tttccgaaga cgaatataaa gaacttcctg
     9661 cgggcgtgaa aaattatgaa ccggccggtg cccttctggc cggcaaaaaa ggaactgaat
     9721 tttacgaaaa actgattcgt caggcacccg attaccttca aaaaaatggt tggcttttgt
     9781 tggaaattgg cgctaaacag gaggaagcgg tgcgccggct gctagacaaa tccggatttt
     9841 atcaagacgt cgccatgcgt cgggattatg ccggattgcc gcgcgttatg agagcaagga
     9901 gaaagaccaa tggataaaat cgtgattaac gggggcatac cgctccatgg taatgttcag
     9961 atcagcggcg cgaaaaacgc cgccctgccg gttctggctt cgtcattact gacggcggga
    10021 accaacacat ttcataatat tcctgatttg atggatatta aaacgataaa aattcttttg
    10081 accagcatgg gcgccgaaat ggaaggcgga aaaaccgtcc gcatccgcgt tgataaaata
    10141 aacaatcccg tcgcgtccta tgatcttgtg aaaaccatgc gggcatcgat tctggttttg
    10201 ggaccgctgg tcgcccgcgc cggggtggcg cgagtttcgc ttccgggagg ctgcgctatc
    10261 ggtgcgcgtc cggtcaatct gcatctcaag gcgctggaag acatgggcgc gcacgtcgaa
    10321 ctcaaaaacg gctatatcga ggccaaagcc aaaaaattga aaggggcaga aatttatttc
    10381 gacttgccca cggtaaccgg caccgaaaat atcatgatgg cggcaaccct cgcccaggga
    10441 accaccgtgt tgaacaatgc cgcgcgcgaa ccggaagtcg tcaatctcgc tgacgtcctc
    10501 aaaggcatgg gcgcgaaaat caccggagcg gggtcggatg tcatcaccat cgaaggcgtt
    10561 tcttccctca agccgacgga agcgtcgatc atccccgacc ggatcgaagc cggaacgttt
    10621 atgattgcag cgggtatgac acgcggagaa atcaatgttc tgggatgcaa tcctcatcac
    10681 ctggaagcgt tgatcaacaa actgcgcgat accggcatga aaatttcgcc gattaaaaac
    10741 ggcctgacag tatcgtgcgg caaaaaaatc aacagcgtcg atgtaacaac gctgccttat
    10801 cccggcttcc ccacggattt gcaggcccag atcatggctt acatggcggt cggttccggc
    10861 ctgagcgtga tcacagaaac cgttttcgaa aaccggttta tgcatgtcag cgaacttttg
    10921 cgcatgggcg ccgatattgt aattcaggga gccaacgccg tcgtccgggg cgttcccaaa
    10981 ctgaggggtg cgcaaaccat ggccacggat ttacgggcat cggcctcgct ggttctggcg
    11041 gcgctggtgg ccgaaggcac aacggaaatt tcccgcgtct atcatatcga ccgcggttat
    11101 gaaaccatcg aaaagaaatt ttccgccctg ggagcggata tcaaaagggt aaaatcatga
    11161 agatgataaa atccagcgct gccggatttg aaaaactttt tcaaaaattg cgcttcagag
    11221 aaggggctca ctcacctgaa ctcctggcag ctgtggccaa aattgtggac gatgtcgccg
    11281 cctcgggcga tcgggcgctt ctgaaataca tgaaaaaatt cgacggctac gcaccggcag
    11341 cagctgagtt ggaagccaca cctgcagaaa agaagaaagc gctggctgaa gtaaaaccac
    11401 aggaaatcag cattataaaa tcggcggcac ggcgcataga acgttatcac cgctgtcagg
    11461 ttgcccacgg atacaaaatc cgtccggaaa ccggcgttga acttgaacag cgcatattgc
    11521 ctctgcaccg ggtgggaatt tacgctcccg gcggcaaggc gtcctatcca tccaccattc
    11581 tgatggcggc cattccggcg cgcattgcgg gcgccgaaga aattgttctg gtcagccccg
    11641 caccggctgg aaagctcaat ccttacattg ccgccgccgc tgagatcagc ggcgttcatc
    11701 gcattttcaa aatcggcggc gcgcaggcgg tcgcggcgct ggcgtacggt accgaaacaa
    11761 ttcctcaggt ggacaaaatc gtcggtcccg gaaacgccta tgtcgccgcg gcgaaaaaga
    11821 tggtcttcgg tcaggtggat attgatatga ttgcgggacc cagcgaagtg gtggttattg
    11881 ccgacgccaa ggccaatgcg tcttttgcgg cggcggatat gctggcacaa atcgagcacg
    11941 atgaaatggc ggcggcattg cttttgacgc catccgaaac attcgccata acggtgtacc
    12001 gggaaatcag gcggcagatt aaaacctcgg cacgaaaaaa gattatcgaa aaatcgctgg
    12061 acaggtactg cgccattttc atcaccggaa gccttgatga agccgtgaat attgcgaatc
    12121 gctttgcgcc cgaacatctg gagttgatga cccaatcgcc tgataaaatt ttaaaaaagg
    12181 tcagaaatgc cggatctgtt tttctgggct cctatacgcc ggaagccctg ggcgattatc
    12241 tggcgggaac caatcacatt ctgcccacgg gagggacagc ccgtttttca tcgccgctgg
    12301 gcgtatatga tttttacaaa cggatgagcg ttctttcttt ctccagaaaa gcgtttgaaa
    12361 aactgagcag gccgacggcc gactttgccc ggatggaggg tctcgacgct catgcccatt
    12421 ctgttctgat tcgcggcaaa taacagttat acaaaagatt tgattgatat catgatttat
    12481 tccgatgctc tggtggacat taccgataaa aacattcttt ttaaaaatta cagtttcttc
    12541 gaccgcaaca gatcggtttt tttcacgaca tcgaaaaatt gtcatcaaaa aaccttccgt
    12601 ccggacgggc aaattccgct ttcacggaac aggggatttc cacgcctggt ttgcgaaaga
    12661 ttttgaaaga cacaaacacg acgggatgtt catcgctttt atccgccgcc aatggtggcg
    12721 gatcggattt acagttgaac gttcagatgc cgttatgaaa atctttcagg aaagagggtt
    12781 gatcagaccg gcgggtaagc atcctttgtt gacgaatcga aagaaagttt gcagatgatt
    12841 tcaaaaaaca acaagtatcc ttccgtaaaa aaagtaaccg atgaggaacg catcggcatc
    12901 gtcagagaca tattttcgac cattaccggc agttatgatt ttttaaatca ttttttgagc
    12961 ctgggtcgcg acatcgcctg gcggaaattt gcggcaaaga aaatggtttt cccccgttcc
    13021 gggaagtttc tagacatcgc ctgcggaacg ggagacctgt ctatcattgc ctgcgccgca
    13081 catcaggata tgtcggttac cggagccgat tttgtccatc cgatggtccg cctggcaaac
    13141 gccaaggtcc ggaaaaaaag actcagcggc aggatcaaat tcattcaggc caacgcactg
    13201 aatcttccgt tcgccgacaa tacctttaac gttacggcaa tggctttcgg aatcaggaat
    13261 attcctcaaa aagaccgggc gattaaagag atgctgcgcg tcacggtgcc gggcgggcaa
    13321 atcatgattc tggaaatgac ctttattcaa aaccgccttt tcaaggccgc ctattatctc
    13381 tatctgaatt ttctgcttcc gctgcttgcc aaactcttct ctaaaaacgc ggcggcctat
    13441 cactaccttg ccgattccat catgaacttt cccaaacccg aagcgtttgc ggcattcatg
    13501 gaaaaaaccg gcgttaccga cgttcaaatt tttccgcgga cgttcggcat tacctgttta
    13561 tatctcgggg aaaaacctgc tcaaaacaac cgcgctactc aaaatcatca ttgaaaatct
    13621 ctgatcagtg cgcttcgtcc cagtttttgc cggaggcaat ttccaccttc agcggtactt
    13681 tcagcctgat gacctcttcc atttcttttt tgaccagcgc catgacgtcc gctttctcac
    13741 ctgccggcgc ttcgataacc agttcatcat gcacctgcat gatcaggcgg gcagacagct
    13801 tctttttcag aagaagcttt gcgatattca ccatggccac cttaattaaa tcggcggccg
    13861 ttccctgaat gggcgcattg atcgccatgc gttcggcgaa ctggcgcacg gaaggaatct
    13921 tgctttttaa ttccggcaga tagcggcgcc ggttgagcag cgtggaaaca aagccgttct
    13981 ggcgcgcccc ctccagaata ccgtccagat aaaggcgcac ttttttatag cggttaaaat
    14041 atccgtcaat gtattcctgg gcggttttca tggacacgcc cagttctttt gccagaccga
    14101 aagcgctcat tccataaaga atgccgaagt tgatcacttt ggcctgccgg cgcatgtccg
    14161 ggttcaccat ctggggaaaa acaccaaaga catcggaggc cgtccggctg tgaatatctt
    14221 catcggaggt aaaggcatcg atgagcgcgt catcttcaga cagatgcgcg agcacccgta
    14281 attcgatctg cgaataatcg gcggagatca gaaagcaatc cggatccgca ataaaggcct
    14341 ggcggattct ttttccctca agcgttctga tcgggatatt ctgcaggttg ggattggagc
    14401 tcgacaaccg gcccgttgcc gtcaccgtct ggttatagga tgtgtggatt ctgcctgttt
    14461 gcggattgac cagggccggt aaagcatcca catacgtgga tttgagcttg gccagcgagc
    14521 ggtaagccag gacttccgcc ggcagctcgt ggctgcgggc caactcggtc agtacgtcca
    14581 catcggtgga ataaccttcc tttgtttttt tcccggcagg caaattcaat ttttcaaaca
    14641 aaatatgctg cagctgcttg ggggaattaa tgttgaattt ttcgccggcc agatgatgaa
    14701 ttttcgattc cgacaacgcc agcagttgcg taagctccgc ggacatctgc tttaacagcg
    14761 ccagatcgag aagcacgcct tttttctcca tctccgccag aacgtgcagc agcggcattt
    14821 caaccttgcg gtaaagatcc atgacgtcta tttcttccaa ctgaccggca agtacaccgg
    14881 taagactcag gatggcttcg gcgcgctgtc ccgcagcggc ggtcatttta tcgagaggcg
    14941 acagcgacag cggatacatt ttgcccttgc ctccggcgat ttcattcagg gacggaattt
    15001 tttcatgcag atattcccag gcgatgtcat ccagttcata agacgatttt gcgggattga
    15061 gaagatatgc cgccagctgc aaatcgtcgc cggagcaaac tgcaagagtg tccagacaaa
    15121 caagcgcggt ttttaagtca taaaaatatt tctttattcc ggtatggttg aaggcgggag
    15181 ccattgcgct caaaacgtct ttgaccgtca gttgccctga gacattggtg tgcccgaagg
    15241 gaacgtaaaa aacatgccta ccggtactga cagccatacc gatcagatgt gccggctgac
    15301 ttttctcata gatgaattcc atcgaaaatt cgtggctttc tttcagacat gcagcaaatt
    15361 cagccagcgc tttctcattg acaaccattt tgcattccag aaccggtttg atctgatccg
    15421 ttctgatttc ctgcaaaagc gaggagaatt caaattcagt gaacagtttt gacagagcct
    15481 ccctgtccgg ctctctgacg acggcatcct gaaaatcgaa atcgatcggg atatcttttc
    15541 tgatattggc aagctgccgg ctgagcctgg cctgctcggc atgcttgcga aaactttcgc
    15601 gcagtttgac attttttaaa ttttcagcat tcttgatcac gttttcgacc gtgccgtatt
    15661 cttcgatcag cctctgcgcc gttttgggac cgatgccggg cacgcccgga atgttgtcgg
    15721 aggtatcgcc catcaggccc agcatttcaa ccaccttgtc cggaccgaca ccgaattttt
    15781 ctttcaccgc atctgcgtca tatgttttat ctttcatggt atcgatcatc gtgacattct
    15841 cattaaccag ctgcaacaaa tccttgtcgc cggaaatgat gaccgtgcgc caacctttgt
    15901 cgccggcccg tatcgtcagc gttccgataa gatcgtcagc ctcggtcccc tgtttttcca
    15961 aaacacaaat agaaaacccc cgaacaacat ccttaatcag cggaacctga gggaccagat
    16021 catccggcat cggcttgcgc gtcgccttgt aatcctcgaa ttcctcatgg cgggcggtgg
    16081 gccctttgag gtcaaaggtg accacaatat agtccggctt cagttcgcgt ttcagtttta
    16141 acagcatgtt ggtaaagccg tatatggcat tggttggaaa tcccttggag tttgtcaaaa
    16201 gaggaaggcc gtaaaaggcc cggtaaatat aattgctgcc gtcaatcaac gctaaaagcg
    16261 gttttctatt gctttcgacc atatcaaatc actttatttt ttatttttta caaatcaaaa
    16321 gatgcattaa ttttaaacgt tctcactgag cgcatatcta gaagatcggt gatgccggtt
    16381 tctcccttga tttcgtttaa aaaaatattg agttcttcgg cgttcgcggc gctgacggta
    16441 aaccagatat tgtattcatg attgcgcaga taattatgcg tcacattctt actggcattg
    16501 acaaccttaa cgaaggtgtc gattttttct tccggaacac gggcagcgca aagcgtgctg
    16561 acacgaccca gttgtgctcc gtcgaagacg gcgccgatac ggcggatgat tccttcgttc
    16621 ttcatattct ggacgcgtct gatagtctct tcttcgctga tgccgcagcg ttttcccaca
    16681 atgagaaaag gctgctcctc cagcggaaat tccttctgta aaatgtttaa tattttctta
    16741 tctattgcat ccataatcta ttttaatttc ggtaatctat tttaatttcg gttggtataa
    16801 gcacaacggc tcttccgcta aatagttgcc tgtagcttca taagcacgcg cgcggcaacc
    16861 gccgcacacc tttataaact cgcagcggcc gcacttgcct tcatatttac tgtaatcccg
    16921 caggttacga aacacttccg aattttccca tatgctcgcg aagcttttct tcttcacatt
    16981 gccgcaatcc agttccagat agccgcaggg ttgcacctgg ccggtgtgcg aaataaagca
    17041 aaatgtgatt ccccccagac agcctctggt cgattcatga aaatgaccgc ccgatttctt
    17101 caccggcttt tcttctttgg gtttgttctg atgcataatg cggaaataat gcggcgcgca
    17161 ggtggctttg agctggattc cacaactcag gctttcctga tgaaaccaca tcagcgtctc
    17221 ttcgtaatca gcggcagtaa tagcctgcgc ggcaagatct ttcccccggc ctgtcggcac
    17281 cagcaagaat atatgatgag cggccgcacc gatatctttt gctaatttaa aaatatcctt
    17341 aatctgatca aggttattgg ttgtgatggt cgtgttgatt tgaaattcca ttttagcatt
    17401 tttgagtgct tcgatgcctt gcatcgcgcc gttaaacgca cctttctgct gacgaaatgc
    17461 atcgtggctg tgcgcgtctt tcccgtcaat gctgatgctg actctctgaa tgccgctctc
    17521 cgtcattttc tgagcaacag ccggtgtgac aagcatgccg tttgtcgcca tcaccatgcg
    17581 cagacccttg ttggttccgt aggcggcaat ttcaaaaatg tccggacgca ggagcggctc
    17641 accgccggtc aggataatca ctggcgttga tacggctgca atttgatcga ccaactgcaa
    17701 gcacttttca gtggagagct cccccgaata agggccgagg ctcgaagccg cgcggcaatg
    17761 gacacagttg agattgcagc tgcgcgtcac ttcccacgca atcatacgga ctttattatc
    17821 aaggacaatc gacataatat tacatttcat ttgctcagca gtttcgccac atccggagcg
    17881 aagtaggtga tgataatatc ggcgccggcg cgtttgatgg ctgtcagcga ttccagcatc
    17941 gctctttctt catccagcca gccgagttgc gcggccgctt tgatcatcgc atattcaccg
    18001 ctgacgttat acgccgccaa aggtaaatca aattcctgct tcgcacggca gataatgtcc
    18061 agataagcca gagcgggttt caccatgatg atgtccgcgc cctcattcac atccagtgaa
    18121 atttctctga tcgcttcgtc tgcattggca ggatccatct gatacgcttt ccggtcgccg
    18181 aactgcggcg tactctcggc agcctcccgg aaaggcccgt aaaacgacga agcgtatttt
    18241 gccgaataag ccataatggg cacatcctcg aacgcgtttt catccagcgc ttcgcggatc
    18301 accgccacct gtccgtccat cattgccgag ggcgccacca tatcggcccc ggcacgtgcg
    18361 tgcgaaagag cggtttccgc gagcacctcc agcgtttcgt cattttgaac gctatctttt
    18421 tccagcatac cgcagtggcc gtggctggta tattcgcaca ggcagacatc cgtaatgacc
    18481 agaatgtcgg gcacgtcatt tttaatacgc ttcaccgctt gctggatgat gccgtccttg
    18541 gcaaacgcgc cggtggccat cgtatctttc ttggcgggaa tgccgaagag cagaatcgct
    18601 ggaattccga gctcctgcac cttttttgct tccttgacga tatgatcaac ggacagctga
    18661 aactggccgg gcatggacga aataggtttt ttgaaatttt taccgggcac agcgaataac
    18721 ggataaacca gatcgtcaac gctgagtttt gtttcacgga tcagacgccg gacgttttca
    18781 ttttttctca atctgcgcgc acgatattcc ggaaattgca ttttctttag tcctcctttt
    18841 tatgaatctc ttcatcggtc agatagcagg cgggatcagg tccccagata tctccctccg
    18901 actctgcccg ggcacggaaa ttgccggcgc agacattgag ccacaaacat cccgcgcagc
    18961 ggccatgcac gtgcttcttt ttctccttga gtttcattaa cagttcattc ttttcatccg
    19021 tccagatttg gctgaaaggc cgattgcgga cattacccaa agactgattg cgccagaact
    19081 gatcgggata aacctcgcca tcccagctca cgcaaccgat acccagtccc gaagaattgc
    19141 cttcgttcat ctcaaggagt tccagaattt ctccggcgcg ttcgggatct tcttttttca
    19201 tcttcagata cagatacggg ccgtcggcgt gattatccac cgtcagaatt tcgggagaca
    19261 acccgtcgtc aaacattttt ttggtttcgg cggcaatcaa atcgatcagc tttcgggttt
    19321 gctcgtggtt taagtcttca tcacgcagtt tggaaccgcg accggtgtat accagatgat
    19381 agaaacacag ccgttcaatc ttttcttttc ttaaaaggtc gaacatatcc tttacgtcgg
    19441 ccagattatg tttgttgacc gtaaaacgga taccgacctt gattcccgcc tcgcggcaat
    19501 tgcggatgcc gtccatggct gccgcaaacg cacctttctt cccgcggaac cggtcatgcg
    19561 ttttttccag accgtcaagg ctcacgccga cataggaaag gccgactttc tgaagtttgg
    19621 ccgcaatgtc tttggtaatc agcgtaccgt tggtagaaat aaccgcacgc attcccttct
    19681 tcgtcgcgta ttgcgccagt tccagcaaat ccttgcggat cagcggctcg ccgccggaaa
    19741 aaagaatgac cggagagccg aaagccgcca gatcgtcaat cagtttcttg ccttcttccg
    19801 tggtcagttc gtcgggataa tcgatgtcgg cggaattgga atagcagtgg atgcatttta
    19861 aattgcagcg gcgggtcatg ttccagacaa ctacaggctt tttgtctgcc gaaaactgca
    19921 acaggtgcga tgggagatca cccgatttgc gattatagcg caaaacatcg gacggttcca
    19981 cagccccgca atataatttg gagattccta tcatttacct ttgcctcttt ttcctgccgc
    20041 cggttttttc ttaaaataat tggctagggc atccactaac ccctcgattg tatattcttc
    20101 ctgatgaata tcaacaggga aacctgcctt ccgggctgcc gctgccgtca ccggtccaat
    20161 acaggcgatt tttactccct tgggcagctt aaaaccgcct cccattattt taataaagtt
    20221 atgcacagtt gatgaacttg taaaggcaat cacatccacc tgattttctt taaagagttc
    20281 ctccagttct tttttctttt tccccgaacc gaccgtttca taggcggtga caacatctac
    20341 ctgggcgccc agttttttca acccttcggg gagaacatca cgcgctttgg ctgcccgtgc
    20401 aataaggatt ttctttcctt tcagatttgt gcgggaaaac gatttcagga ttccttcggc
    20461 aatgaattcc ttgggaacaa gatcaacccg gattcccttg tcctgaacct gcctggccgt
    20521 tgccggaccg atgcagcaaa ttttaatacc cttcaaatcg cgaatttctt ttcctttagc
    20581 tgaaagtctc tcaaaaaaat aagcgacgcc gttggcactg gtgaagatca gccagtcgta
    20641 atcctccagc tttttgattg cgttatccat ttcgcgccag cttgccggtg gaacaatctt
    20701 gatggtcgga aaatgaatgg ggtttgctcc tgctttgatc agcagttgcg ccagatcatc
    20761 ggcctgcttc tcgggccttg taatcacaac acctttgccg aaaatcggtt tggaatcaaa
    20821 ccactgcaaa gtgtcacgga gagcaaccac ctcaccgaca acaaaaatag cgggtggcgc
    20881 gaatttttgc tctctggcca gtttgacaat gctggacaaa gttccggtga gaatttcctg
    20941 tcttgccgtc gtgccccagc gaatcagcgc ggcgggtgct ttaggagacc tgccgttatc
    21001 ctctaattcc ttaacaatct tttcgatatt ctttacgccc atcagaaata ctaaagttcc
    21061 gattcgggcc agtgcctgcc agtctatatc gctttttccc ttggtcgggt cttcatggcc
    21121 ggtgacaaaa gccaccgtgg aggtcaaccc gcggtgcgtc agcggaatac cggcataagc
    21181 gggaacggca atcgcggatg tgacaccggg aacaatttca aaaggaatct tgttagcggc
    21241 cagtttttcc gcctcttctc caccacggcc gaagataaat ggatcgccgc ccttaagacg
    21301 ggcaacgatg tttccggcat tcgcttcttt caccagaaga tcgttgattt gccattgcga
    21361 cagagtatgc gcgccgccct gtttgcctgc gtaaataaaa cgggcatcgc tccgagcata
    21421 ttttaataaa tctttgccga ccagataatc gtaaatgacg acatccgctt cccgtaggca
    21481 atcaataccc ttgagcgtca tcagccctgc atcgcccggc cccgcgccaa taatataaac
    21541 ttttcctttt ttattctttt tcataattgt ccatttgtca ttccgaacgt atgtgaggaa
    21601 tcttaaaaaa ccaattttat aaaaacaaga tttcccgctt cgcttcgaaa tgacatatta
    21661 atatcagcac atcaaatcca tcagtcttcg gccgcctttg tccagaacga tttcagccag
    21721 ttttttgccc atctcttcgg catcttttat ggtgccgctt attttttcgc gaataacagt
    21781 ggtgccgtta ggcgcggcaa gcatgccctc aagtgtcagc atctgaccgt cgagtttgcc
    21841 cagcgccgcg atcggcaggc gacatccgcc gcccagcgtg cgcagaaatg cgcgttcggc
    21901 ggcaacttcc gccgaggtcg ccgcatcatt caatacggca caggcttttg ccagttcatc
    21961 atcatttttg cgtatctgca accccagtgc gccctggcct accgccggga gcatcgtttc
    22021 caccggcaga aactgcgtta tcttttcagt cagtccgttc cttttcatcc ccgctgccgc
    22081 taaaattacg ccgttgatat tttccgtttc aattttttta attctggttt caatattgcc
    22141 cctgagcggc acaatactca catcaggcag caacgccttg atctgcgcgc cacgccgctg
    22201 cgatccggtg ccgatttttg ctcctttcgg catgaattcc aatttgatgt tgtttcgcga
    22261 gaccagaaca tcgcgcacat cttcgcgcgg cagaatcgcc gagaatatca atccttccgg
    22321 attttctccc ggtacatctt tcatgctgtg taccgccaga tcgatttttt ccgaaagcag
    22381 agcctcttca atttccttga caaaaacgcc ctggccgccg atggtcagaa gcgacacatc
    22441 ctgcataatg tcgccgctgg tcttgatgac gcagatttcg acttccgcat ccggtatggc
    22501 cttcttcaat ttatcggcaa caaagttcgt ctgcgtcaat gccagcttgc tgccgcgtgt
    22561 acctatttta atatttgtca ttcaccattc atcctttact ttttactgct tttacatcaa
    22621 tccaatcgaa acagtctccg cacggccgcg acgatgtcgc gggagttgaa ttcggagctc
    22681 tcttccttca tcaccgatac cggagaatgc agcaccttgt tcacgatgga gttgatcaga
    22741 atctcgattt tctcctgatc ttcttttttt aaatcattca tccagccgga agctttttct
    22801 atttcggcct tgacgatatc atcagccttg ttgcgcaggg agacgattgt cggcacggat
    22861 tcgagttcct tctgccagtt gatgaatctc tccaattctt cgtctataat tttttccgct
    22921 ttgatcgcct ctttccggcg ggcgttgatg ttttcgtcaa cgatgtcctg aaggttgtcg
    22981 atgttgtaca gataaacatt ctccagctca ctggcgtccg gatcaatgtc gcgcggaacg
    23041 gcaatatcaa tcagaaacag caggcggttt ttacgccggt gatgaatttt tttaagcata
    23101 tcagcggtaa caataaaatc aggagcgccc gtggaactga tgacgatatc agcctcaatg
    23161 agcttcttct ccaaggcatc gagcgccacc gcttcaccgt ggaatttttc cgccaacagt
    23221 tcggcctgtg ccagcgaccg gttggccacg ataatcgact gagcgccttt ttcaatgagt
    23281 tgtgtgccgg tgagctccgc catctctccg gcgccgatca gcaggattat tttgccggta
    23341 agcgttccaa agattttctt ggccagctcc accgcggcgt ggctcaccga aacgggattg
    23401 acggcaatgc cggtttcgga gcggacgcgt ttggccgcgc ggaaagagca gtgcatcaga
    23461 cggtttataa cgacgccggt agcgtattcg gccagcgctt cgcgataggc atccttcacc
    23521 tgcccgagaa tctgcgcttc gcccataacc atcgaatcaa ggctggacgc cactcggaaa
    23581 atgtggcgca ccgcgtcttc gcctcgatat tcataaaagc tattggcagc ttcggcctgg
    23641 ttcaaaccgc cgtttcgtgc caggaatccg ctgagttctt tgagagcgtt gccgtcttcc
    23701 gcaacactgg cgagaatctc cacacgattg caggtggaaa gatacagcac ctcctccacg
    23761 ccgccgatgg aacgcagcgc cgggagcaaa tcttttttct cctgacagcc ggcgaaaatt
    23821 ttttcccgga cagctagggg ggctgtttta tgattcaggc cagtcagtac aatcatattt
    23881 aaataaagtt atgcaacgtc ggacaacaaa ttttcacgcc aacgtaagac acgataagaa
    23941 gaataaaggt gatcccggaa atgagagcca tccggtaacc cttccagccg atagccagac
    24001 gctgatgcag cagaaaaccg taaatcatcc aggcggcaaa cgtccagatg atttttggat
    24061 cggtcagcca gccggttttc caactcatct ccgcgaaaat cactccggca ataatgccca
    24121 cactcaaaat cgggaatccc cataaaagac acaaatggtt gattctatcg agatcgttta
    24181 aagaaggcag aagtctgctc ataccgctta acttcttgct cttcagcaga cgattttgca
    24241 tgatgaacat caatccggcg caggaagcga tgacaaaaag cacttccccg aaaatggtca
    24301 aaaataaatg ggcggcggtc agcccgcttt gcagattctg cggacgaata gatttgcccg
    24361 cttcctctcc ggcggccgca atcaaaaaaa gcaggatcaa cggcgaaata aaggccccga
    24421 gcactctggt tttcgtttta aattgcaata ccagataaac tgcgcacacc gcccaggcac
    24481 aaagggaaag aaaatccttg gagtcgggat ttacaaacca agaagaatta accatgacaa
    24541 aaaagaattt aacggtcaga aatgcaaaag cagcggcaag aacccaagtg gacattttag
    24601 ccagttttac ttttttgaca agaagcgaag acagataacc gatggtgctt gcgccgcaga
    24661 ggacaagggc agtgttaaac gataacattt caagattaac gaaactcaca ttccacctcc
    24721 tcaccggtta ttttcttgac gatatcctgc actttctggc tgtctttggc tttgatcaat
    24781 tccaggatgc ctgccgccat caaggcatca aaccagtctc tgccgattcc ggcgtttttt
    24841 tccatcttgg tgcgcagggt tcctaaaata ttcagcaata ttgcgtattc cctgccgtat
    24901 tgttcttcaa gctcttcccg taaatgacgc gccagtgccg ggcttttgcc gccggtgccg
    24961 atagccatga ccagatcgcc tctctgggcg atggcgggca gaataaaatc gcatttttgc
    25021 ggatcgtcga caatatttac aggtatgccc ctggcccgtg catcctgcgc tatgagggcg
    25081 ttgatctttt cgtcatcggt cgccccgata atcaggacag cccggtcaat caagtcgccg
    25141 gcatattcgg cggcaatgtg ggaaatcaga ttcttctctt tgagcgcggc aagctcccgg
    25201 acaagtttcg gactgataat ggagactttc gcgccacact ccagaagtct ttcagctttt
    25261 ctgaaggcga cttcgccgcc gccgacgatg acgcaaattt tgtccttgat gtccaaaaaa
    25321 acagggtaat atttcaatag ttttcgtcct ttcgcccgac gggccgcggc caaaagcatt
    25381 ttgggaatag ctatcacgaa ggggggtaaa attcaagaac gtataagatg aaagcatcgc
    25441 ggcgtccatt tatggacgcc gtgatttgtt caggttttct ctacgaagaa actgttaaaa
    25501 gatgaaatcg agtagatgtg tggtcacctt ttccggttcg ccgtcatttt gtacagctgc
    25561 cgccgtcagg acgtccagat gattatatcc ggggataatg ggcgaatccg gcgacaactg
    25621 gtccaaaccg agtttcgcgc cgtcacccgc aacaatatta ataacgggcc gttgggaaat
    25681 accgtccgga tgcacgatca catcgctgcc catcatagac tcgaccgcca gccgcatcgg
    25741 aaaatatttt tccacaaagt ccatcggcag cgcgcctaaa gaccgcgcca gatcctttgc
    25801 atcggttact tccttggcgg cagaagtaaa gggcacgccg tcattgttgt tcggaatcgc
    25861 agacccttcg atttcatcgt aattgatcca tcgatagagc ggtccttttt tcagggcagt
    25921 accgctgttg tttgcaatgg cgagcgttcc cccgcttccg aagggaaagt gtttttccgc
    25981 aaccgttccc cccgcaaaaa aacccatgct tgcctgaatg atattaatcg gcattgcgtt
    26041 gtcatccata aacagggcca tcaaagcctg attggtgtac cggaacttgt agatggaggg
    26101 agtgaccaat gcgtaatccc atatattttt tgaatggtaa aaccggtagc agtttttgac
    26161 actggttgag gccggcaaat acgatattaa atctgtttct gcattcggtg cgaccatggc
    26221 agcatagccg acaccggtca gtagattcat gatttcagga gtaatgacgc cggaaagaaa
    26281 gataaatcgt tcaaacaaac ccttccgcat taaatagaca gcgctgtcgg gtatcttgct
    26341 catcagatag cgcagagtgg ccgagtcccc tccctgcacg gaaagcagcg gatcggatgt
    26401 aatcagggaa tccagagcga aatagccggc agcctgattg tatcccgcgt cggcaagtgt
    26461 tgtcggatcg ccgtcaaaat cggcacaggc ataagcgcct gtcagaaatc cgccaagaga
    26521 gtgaccgccg atgtagactc tctgctgacg aaccgtcggg tcgggaatgc cgcgcgtgat
    26581 gatctcgttc cagtccttca gggtctgctc catgcccatt tcaacgagcc atttcacgtc
    26641 gtcgctcaag ggtttaaaat acccctggaa atactgcccg tggtacagtt tgttccggta
    26701 ataataatcc accatgtcgc gcagattgcc ggtttccctt gccagcctga ggccgttcat
    26761 gtcttcgaga caatttgatc gtctgtcaat cgcccaaaat tccacaaatt tgcctttctc
    26821 atcgtatgct cgcgtcacca gattggccgc gacattgtag aatgccgatg cgccctccag
    26881 aatacccggt tgcgcgatca gaattctatc cgcgttggag ggattgtccg gtccgtgaat
    26941 atgcctgatt ctgagaaaat tgagtttgtc gcaggctgcc gggtgggatg gacacgacgc
    27001 cggcaggggt gcaaaaacag tcacggccgt tgccttcgtt tgcggaaacg cgatgttttg
    27061 gtccgccacg ctttccgtga agcctgccgg aggccagacg gcagtcatgc cgcttacagc
    27121 cataaaaaaa accaatacac aaacagataa aaatacaacg atttttttca taaacccctt
    27181 cctcccctga tttagaatta agaacgaaga aaatcctgca cagctaacta tctttatttt
    27241 aatgtaaaaa atttatatta tatggatttg tccggaggga gaaacgtaca gtgtgagatg
    27301 aagaacgtac aatttgttct cttcttaaaa taaattgaaa atgtttgtca agcgggaaaa
    27361 acaaacgaaa aaattaattg gacgggcaga gattttttct gaaaataatt taaggatgat
    27421 cccgaatcag agtcgaaggc gacgggtttt ctgctattcc gaatactcca cgcggttgcg
    27481 tccgttcttt ttagccttgt acatggcttc atcggcgcgg aaaatggtcc ggtcaatccc
    27541 ttcatttttt aaatgttcag ccaaaccgat ggaaaccgtt accttcaatt gacggtcggg
    27601 ctccagatca aaaacgagac aatcttcaat gcaggcacgg atacgttcgg caaaaattct
    27661 ggcctcttcc agatctgttt gcgtcagaac aatcagaaat tcctctcccc cgtagcggcc
    27721 gcagaaatcg gtttttcgca aaacattgcg gatcacattg gaaaacgtca tcaaaacatc
    27781 atctccggcc tggtgtccga acttgtcatt aatatttttg aatttatcaa gatcaatcat
    27841 cgccagagaa aaaaccgcgc cggtacggac cgagcggtta tgctcggatt caagaagctc
    27901 cataagatga cgacgattat aaaagcccgt caggtcatca tgtatcgcca tggcctggat
    27961 tttttccaca gaggcctgaa gctgcttgtg gctcacagcc agatcacggc gcaaagcgct
    28021 gatgttgccg ccgatgaaag aaacggaagc aagaacaaca cccagagcga accagtaaag
    28081 aacctccaat ttgagatgaa tagcctccgg ccggtaaatc tgaagcagac agatatccac
    28141 cgcatatgtc cccagaataa acgcgctggc ttttaaaaac tgacgtgtgt ccaggcgaaa
    28201 ggttccgaac aacagaataa gaacatacac cgcaaaaaga attcccttgg cttccggaac
    28261 aaagaacagg ccatacatga caaccagact ggcaacggat atttgggcaa aggtcaggct
    28321 gggatccttc attcttttat taaggccgct gcgaaaaacg atatacaaaa taatcatcaa
    28381 aatcgtcgtg atactccaac cccatacgag ctctttaaac tccatcaggc cgccggtgta
    28441 cgccagataa gaaagcggca tgttcagaag atagacaaat attgaaatga aaaaacgttt
    28501 aatgcgcaaa gcctgcttgg gatcgttatt aggaatgagg gaaaagtttt ccgctaattc
    28561 ggaaagagaa taggccagtt tggaaaactt tctcgaaata ccgtttatat tcaaggacat
    28621 tcttttataa gccatatatg caaaaagctg tttttcaaaa agattagaag aataggtgac
    28681 tatgcgtttc cttaagcaca aaacaaacga gaacgccaca taaacttaat ataattttta
    28741 aaaggaagaa accgtaaatc agcaatgatt ttgttgcgga tgcaatgccg cgatttcatt
    28801 ttatctggtt acgatccgga aaatggtttc caatttcgac acgtcaggat tggatagttc
    28861 gaccagcaag cttcagtgca cttttcctag aatcccacgg cgctacgcgc ctggggatag
    28921 ttcgccttac gcttcaaact tcgctgcgct cgttttcagc ttggctcaca tcccgcgcca
    28981 cctcgcttcg gggatagttc gccttacgct tcaaacttcg ctgcgctcgt tttcagcttg
    29041 gctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnactaaa atatagttcg
    29161 tcttccgttg tatctttcgg tgtcgtagta tttttccacg tcaacaagtt tcagtttatt
    29221 gactactccg gccaaaggcg ctgtgccgat atcaccgcgt tccagggtaa ccatgcgtcc
    29281 gaaatcttcc ttgacaatca tgtccaccgc cgcaatgccg aaccaccggg ccatcatacg
    29341 gtcatgcgct gaaggcgttc ctccgcgctg aagatgactc aggctgatat gacgggcttc
    29401 caatccggtc ctcttttcaa tttctgcggc aacaaaactg cccgctccgc cgacggagac
    29461 atgaccgaaa tcatcaatat gagaatcttt taaaaattcc tgtttccccg caagtttggc
    29521 gccttcggca accacaatga tggaataatt tatttcccgg ccttttcttt cgcgcaacag
    29581 caagcagact ttatccatat cgaagggatg ttccggaatc agaatgatat aggcgccgct
    29641 gcattcgccg ccgcgcagcg cgagccaacc ggcatgccgt cccatggttt ccaccacgaa
    29701 gatgcggccg tgggaccccg ccgttgtccg gagacggtca atttcctcag tgataatgtt
    29761 aatcgccgtg tcaaaaccaa gcgaatattc cgtccccgcc agatccatat cgatggtttt
    29821 gggaatgccc accgttttga tgcctatttt atgcagtttg tacgcaacac ccagcgtgtc
    29881 ttccccgccg atggcgacga tggcgtcgat gccgagcttt tttatattct caaggacccg
    29941 ggcggaccgg tcatttttag gattaaaggg gttggttcgc gatgtcccca gattcgtgcc
    30001 gccgtatcga tcccatgtcc ggacgatttc ttcgtccaaa tgtttcaagt ggcgggcgcg
    30061 gcttttttca tcctccggat caacgtcgat aagccctttc cagccttcca caataccgat
    30121 aacgtcgaag gaaaccgacc ggttggccgc cagccgcgga tcgagcgcgc tttttgtaac
    30181 ccatttaatt gccccgttca aaccgggaca atctccgccg ccggtcaata ctgctatttt
    30241 catcttcata ttttctctcc gcaatattga aagttaataa aacactcgct aataattgac
    30301 ggatcctgac ggaaaaatcg cgatgtccat tttataagaa aaaccggctc ttctgtcaac
    30361 cctatattcc cccgggacgg aggatattct tttcatgttt tcgtcaggac gcttgaaata
    30421 ttacttaaaa aaatatatat tttataaaaa aacaggacgg gctttcatga cagaaaaaga
    30481 aaccggcaaa tggctgaaaa tcgatatatc ggctcccccg gaactgatgg aggcgctggg
    30541 aaattttctc acggaatccg gtgttcaggg cgtgtttcag gaatcgcagt cctctcaggc
    30601 cgtcggttca gactttcccg aaccggttgc ggaagacatc ctgaaagcct atttcccgct
    30661 cgacagccgc agcgataaaa aactggcggc actgcaaaaa tacatcgaca gcctggccga
    30721 aatattcccc gatctgggaa aaccgtcatt tacaaccgaa accatctgcg atcccgactg
    30781 gggagaacag tggaaaaaat attttaaacc catccgggtc agcaacaaca tcgttgtcaa
    30841 accgacatgg gaacgataca cgccttccag tcgagacatc gttattgaaa tcgatcccgg
    30901 catggcattc ggcaccggcc agcatgcgtc caccagaatg tgcattgaag cggttgaaga
    30961 tatcatcatg aacgaccgtt ccattaaaga atggaaagtg ctggatgtcg gttgcggcac
    31021 cggcatcctc ggcattaccg ccgccaaaat gggagcacag gatgttctct gtgcggatat
    31081 cgaccggaaa gccgtcgaaa ttgctggtga aaacgcggcc attaatgatg tgcggcacgc
    31141 cgtccgcacc atcaataaaa atctggctgc cattgacaaa ccccgtaatc tgattatcgc
    31201 caacctcacg tcgaatctac tgctgaccca ccggcagcat ctgacgcaat tactgatgcc
    31261 ggacggctac atgatccttt ccggcatcat tgaacaagat gccggaaaag tcgcgcagga
    31321 atatgccgca acacctcttg aactgcaacg gacaatcacg gaaaaggaat gggtctgtta
    31381 cgtgctcaaa aagacatccg cctgctgaca gcaccctgca aacggcaatc ttcagataaa
    31441 ccatctataa aggaaaacag gccttgaccg tcccccgtat atacagtccg gaatctctgg
    31501 aaaataaaaa cagctgcgcg ctgggagaag acaatgtaaa atacctgaaa aaggtcatgc
    31561 gcctcaagga gggcgaccgg atacatattt ttgacggctt cgggcgggaa tacgaagcga
    31621 ccatcaaaaa tttttcggca aaatcggtgc tcgtcgactt ggggaaagcg attccctccg
    31681 ttaacaggga catcagcatc accctggcgc aggccatcac caaagggaaa aaaatggaca
    31741 cgatcgttaa aagcgccgcc gaactgggag cggacatgat tattcctttt catgccgtgc
    31801 ggtcggtcag ccatattgcc gatgaaaaaa atccccggaa acagacccgc tggcagaaaa
    31861 tagcgcacga ggccgcccgg gcgagccaca gcgcttacat cacggaggtt acaaaaattt
    31921 tatcttttcc ggaaatgctt tcccggtgtt ctcccaaggc tttgaaactg attttctggg
    31981 aagaagaatc ccggaagacc atcaaggata tgctgacgga tgacactctg gaggcctgcg
    32041 gggatttttt tattgtcgtc ggaccggaag gcggtttttc aaaagacgaa atcgaaaaaa
    32101 caaaagaggc cgggttcttt tccgtcagcc tgggcagaca aatcttaaaa gtagaaacag
    32161 cggcaacggc aatcatatcc atcattcaat acgaaaaagg aatcttcagt tcaacatcac
    32221 aggaggcatg acatggcttt tcatccatac gcaggtttct ggcgacgtct ggtcgccttt
    32281 accatcgacg gtaccattat ctgtttcatc tttatctttc ttttcttcat tgcctcaatg
    32341 gcattcttca gcggcgccgt atcagccaac ggtgaaaaat ttctagccag cctcggcaat
    32401 gccgaatctc tttctcccgc gatgatttct ctgtggttat tttatattct gctcaatatc
    32461 ggttatttta catattttca tggcgcgacg ggccgcacac cggggaaaag ccttctgggc
    32521 atgcaggttg tttccacgga aggaacaccc atttctttcg gaacagcgtt tctaagatcg
    32581 gtgggctatc tggtatcaaa tatcttatat ctcggtttta tctgggttgc atttgatcga
    32641 cgaaaacagg gatggcatga caaaattgcc ggaacggttg tcatcaggag agaaagttac
    32701 gaacaaagcg ctggcgtatc catcgccgag ccgacatggt cgccaccgaa ggcacaacag
    32761 acaaaaccgc ctgaaactaa tgaaaattag acggcaaaca gccgggaatt tcacaaaaag
    32821 gccttgacaa aaggacgctt attctataga cggagtcagt atttttaacc aatcgggtcg
    32881 gtagctcagt cggtagagca tcggactgaa aatccgagtg tcggca
//
LOCUS       DAOSME010000025        32376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1807_length_32376_cov_20.134928, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000025 DAOSME010000000
VERSION     DAOSME010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 32376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32376
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1807_length_32376_cov_20.134928"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1415)
                     /locus_tag="PLG98_05470"
     CDS             complement(<1..>1415)
                     /locus_tag="PLG98_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32737.1"
                     /translation="LGFYQHGYRFGVAALKTNEKIADTKNRCRIEFAFPMFIQHWKKH
                     ARKDLDYFRNAYKNGIENGDLIFSGHNVNLIGMTRIMLGDNLDDILEEYGKYRDFQLG
                     GKDPFIARNYMENTRMCLCLKGRTESRGSLNGDGFDAKEQAGYYKSENNLLGTFYFSL
                     VRLRINYLFGNYSACRDLLPEMRRIVQKKTALGNLHIPEFYLYSSLTMTASYPEADPM
                     TKARYKMSLRANQMKMLIWAKACPENFQHKYDLVAAEMMKNKGRFREAQKLYHKAIEG
                     ARENGYRQEEAIACERLALLYLDHRCRDEAGFFMQKAHKGYLGWGASEKARDLEEKYP
                     SLVPLEQKQWGTGTVTVSGPSGSMTISGATETTGSTKMLDLSTAMRVSQIIASEIMLD
                     RLLQKIMHESITNAGAQRGYLLLESDGDLTIEASQDVDKGESQVMQSLPLKDCPDICH
                     SIVNYVYHSGEDIVLGNAVKEG"
     assembly_gap    1416..1515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1516..>3033)
                     /locus_tag="PLG98_05475"
     CDS             complement(<1516..>3033)
                     /locus_tag="PLG98_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32738.1"
                     /translation="FLIGAYRDNETNDAHPLTLALDHIRKKGKKINSIVLGPLNLTGI
                     NMIIMNVLLCDAEKSMPLAELINRKTAGNPFFVIQFLKNIYDNRLLQLNPSTGWEWDI
                     DKIRQMQVTENVIEFMAEKISHFPKNTREILKICACIGNRFDLELLSVVSGKSIDETL
                     FDLTSAIQEDMISLHGNMYKFMHDRIQEAAYSMIPEEEKSAMHYRIGGNVLKKTDEKN
                     LHEKIFYIVDQLNRGIRCVSGTDEEIMLARLNLQAAQKSKKSTAYGSASFYLKTAMGL
                     LPEYAWKSHYELTYAIYKEAIECEYLNLNFSEAEKIFHAVVNNARSNIDKANVYTLMI
                     ILYTTQGNYDAALHVGMEGMKMLGIHLPDNVSDVRVGWELLKLRLKFGRRKIEDLIDL
                     QYIPMPNTLDEAKQMAAEYLRVRPGKSLASPEINLWEMLSYAYLAIHTGTVAFYANPN
                     LFAFIAINGVYRLLDYEINFEYSPFAYIALGSIVGSSLGFYQHGYRFGVAALKTN"
     assembly_gap    3034..3133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3134..4297)
                     /locus_tag="PLG98_05480"
     CDS             complement(<3134..4297)
                     /locus_tag="PLG98_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOE32739.1"
                     /translation="MEKFANYILSEKIHETRNSVIFRGYKETEGLPLIIKLLKTINPT
                     SSEIARFKQEYNLVKSLDVDNIVKIFDLVDYDNQYAIIEEDFGGISLKDSLKDKELDL
                     QSLLRISSKVSGTLGLIHKNNIIHLDIKPDNILINPERDEVKVADFGISAVLTHANDE
                     LYNPDVIEGTLSYMSPEQTGRMNRGVDYRTDIYSLGVTFYEMLTGEVPFKSKDPMELI
                     HSHIARQPLPPDHLNPSIPPVLSSIILRMLSKNPEERYQNCLGIMADLDECLRQLNQK
                     GQVDDFPIATKDISIRFNIPRTLVDRDNERIELMRCFERTCKGLSEFMLVTGQPGIGK
                     SALVNEIYKPIIARKGYFIFGKYDQFRKDVPYSAIIQSFQGLIRQIISESEEKI"
     gene            complement(4410..5507)
                     /locus_tag="PLG98_05485"
     CDS             complement(4410..5507)
                     /locus_tag="PLG98_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE32740.1"
                     /translation="MKKCSACGEVQEANAIFCTNCGQRLAEDSNVKKCPSCGTSNKPE
                     DAFCSDCGSRLEGPKAQASESSPVATQLLALNNEFLSVRESSAGQFEFSSETGARSAV
                     QHVKIKYTALAILDPLTKQITFWEKMVESSAGMTAGVFAEKTVQKGIDVGKKIHGQLL
                     FGGKYGFEYGKLKDVVKDIAAKHGWNFKLNIFKPKSKTETTGLINKIPFIKVLGPIFA
                     ILLIMAIGLIAYFTLSGDSSKTTSQAGLESHDSDEVGKAGSSIFGKSKTEADSSKQII
                     QTDRDTYRSGEKIQVHYYNAPGHSRDWICIVPAGSRNTDAGDYQYIPRRGQGVLTFRS
                     PGPGRYEARAFYRYSPGRYRITARYTFTVEG"
     gene            complement(5603..6364)
                     /locus_tag="PLG98_05490"
     CDS             complement(5603..6364)
                     /locus_tag="PLG98_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOE32741.1"
                     /translation="MTHNENRIVIIAALIVGICFLMGIAIGGYFIGKGGTRFKSDTRT
                     VTVKGLVEKVVKADQSIWVLRFRRASEDIKDAQTKISADRDAGIAFLKKQGFKDEEIS
                     RQPTRTIDKLAREYDQSQATERYRYVVTSSLVVTTSNIDLVIKALGETEELLKSGVVL
                     DGQDEGTANPRYVITKFNELRPPLLAEATKNARMTAQQFASDSGARVGKIRSANQGNI
                     QIFGSDGNDESAPYSPTSTPVKKIRVVSTFEFELI"
     gene            complement(6562..7077)
                     /locus_tag="PLG98_05495"
     CDS             complement(6562..7077)
                     /locus_tag="PLG98_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOE32742.1"
                     /translation="MSELFEKVKLGISKSVAKASVKSKEFLDVTKLKNQISSLQNKKQ
                     DALEELGNIAYAMFLKNNIDTDIIKNKGNEISKIDEQIKNLQNEIVEVQKRAQDSLRE
                     DVPSANTCACGAVIPEGAKFCGVCGSKVETASMSGEHPKDPILESRCKQCQTELEPGA
                     KFCAMCGAKVS"
     gene            7330..8058
                     /locus_tag="PLG98_05500"
     CDS             7330..8058
                     /locus_tag="PLG98_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOE32743.1"
                     /translation="MPNIQFFALAESVCKKLNLPPIPFPVRKGRDEIIFNPQGVALDL
                     LLDELEQYLIEKPEEKIFYKDAFSRLAAIEGIRLGEEGFHDHAAHYFELGLALDPANL
                     ALRSNYALSLQSAGRKEEAMRQYRFLLQQPVISGQCLVLIPAARLFLDCGDPVTAHQI
                     VKHCASFMPVDNEFWDLFAEARQRCGIKRWMAPRQKQQEIMRQATAPDYKSAPTGKKK
                     NFCPACGNRIKPEARFCGGCGRAL"
     gene            8048..11428
                     /locus_tag="PLG98_05505"
     CDS             8048..11428
                     /locus_tag="PLG98_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32744.1"
                     /translation="MRCKNDFSVECMKAKAFAVQSVLYAFRVFTPVLILLIILLLFVS
                     HAIADASDYRDKGKENWVLFTFILKDAKTGEPIKGRVYYQSDLAADWFHPDNTKPLLP
                     PGIYRVVGWERHYWQSEQKLIVNPLKGLQQQIVIDLEPVSSPRDGKSGSPLKVPPILS
                     NLIITGNAAVKMPHMNASWFGQSSMATFHVYTNAWSEYPGGIGGGSRNSTGHIDYYFF
                     LHTEDAQKHYERLRPGFKVIPNSSQHVSGPTPVSLGDEALQISIDTERPDGKGLLYES
                     RKYLIRDGHFVMNMYINREIGLDFLTSPEEILKRVRALDVRQVKQMLEPEPYPWPEIT
                     PTPGSGKDVDSLGSKNEVADYASVSPEKALIAVAGVSMVLTLGVLVQLWGMGGLQSPG
                     DIRGALEGLFSSSGGEVTETESSVPETGAVQEVEVIPKEPEALVPETVIQESRVVETV
                     IPDPVPSGFEYQGKVWYKPPWDKGGPYWMDKSDYDAMRSMMRRGMVWSDRWGWVTPED
                     GKTMEAQRVAAWDEYKSNTDKDIKVLTDRIAESQEKLAEIREKQAELERIEQLRERLT
                     ELERQRELDNSFATQLSDTWDNYVEGVNRDLDALPGELGDMALDAARGIRDTTGALIT
                     AAGDTVSDLTNGDNWKAVGKAAVDTAKDLILHPLESSVKVGDVVKDAGSIAIKAADVA
                     GNVAVAIVSDPFAFAESVVGIDNWKKVLDPNVPVGERVSRALYGAVDTALNFASGGTK
                     TAAAGVEAGMDALKTADTVSDAAKAANAADMAADAARITPKLDDPARVQVWEVGRQAG
                     QQKADKLAEAIKSGKPSEIKQALVECQKDKHAIGALNRADDTVKHAYNQQMKQLITDD
                     VDKVVKEKIAQRYGLDPKDVEIVKPTNPTSKPKVGSDLDFTVKVKAKPGEIVPDPNKP
                     GSFIHVKPGDNLTKDVPLKDAQEIYNRELYSKVTGEPIPTDPEKFKAFQAEADKLAHE
                     MDHVAGHRVGAESYGASQQDLEVILQKSGGRFTDPEQAGMVASYKSTHLYQEAESITK
                     LDPGKAEQLVADGMRQTTKQYSNLIETRVAALQNQGIEVAVDPRLTKAVDIFKQVETK
                     GLPPAKAEALLKSQLNMSKDDVANMLGAQLAKLHRVKA"
     gene            11455..11919
                     /locus_tag="PLG98_05510"
     CDS             11455..11919
                     /locus_tag="PLG98_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32745.1"
                     /translation="MWAVFLKIVSYFFLLLFVLGVVLMLLTWRKEKFVKPKWILFGQI
                     VAFMALVVFTMLSRNPLGFWSWLLIFLAGLCGGYFYGRTVKVKKSARGIMMNYTLPYV
                     ITWGVLLFLTQFLTISTGRIPVIVLGLCVLNTGLNLAMNGQVVWNYNRMKKT"
     gene            complement(11983..13170)
                     /locus_tag="PLG98_05515"
     CDS             complement(11983..13170)
                     /locus_tag="PLG98_05515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE32746.1"
                     /translation="MSIAKKIKSSISNSSMIRKMFEEGAVMKRKYGADKVFDFSLGNP
                     NVEPPAVFKRELIRLAAKPLPLKHGYAPNAGFADTRQAIAKQVSKTCDLNLSADHIVM
                     SCGAAGALNVIFKSLLDPNDEVIVPMPYFVEYPFYIDNYGGVTKFVRTRKDFSLDIEA
                     ISKAINKKTKAVLINSPNNPTGKVYSSKNIQALAALLKKKGKTFHKTIYLVSDEPYNE
                     IVYDRVIVPSVLKSYTNSIVACSYSKTLSLPGERIGYIAVNPEIDDCRDLISALILCT
                     RILGFVHAPALMQRIVVKLQNAAVDVNVYQNKRDLLCKGLSKAGYKFAKPQGAFYLFV
                     KSPLPDDVAFTKILLQKNILVVPGSGFGAAGYFRIAYCVDDKTIVNSMRGFTEAIRET
                     SGK"
     gene            complement(13179..13490)
                     /locus_tag="PLG98_05520"
     CDS             complement(13179..13490)
                     /locus_tag="PLG98_05520"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOE32747.1"
                     /translation="MSDKKQNVITGGTVLITLGILIYLSKSGSFPFGQTWPVLLIVIG
                     ICTLIQKFRDLGGWIITLAGTGFLFYEFYGFDVSQYSKYVLPGIIILLGIFVLLRRKK
                     G"
     gene            complement(13549..14304)
                     /locus_tag="PLG98_05525"
     CDS             complement(13549..14304)
                     /locus_tag="PLG98_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOE32748.1"
                     /translation="MLGTLSMPTLVVIVLVVMFLVSAIKILSEYERAVIFRLGRIRDA
                     KGPGLIIIIPGIDKMVRVDMRTITMDVPPQDVITKDNVSIKVSAVVYFRVMDANSAVT
                     NVENYLYATSQLAQTTLRSVCGQMELDEILSEREKINMNLQEILDRSTEPWGIKVSLV
                     EVKHIDLPEEMKRSMAKQAEAERERRAKVINAEGEFQAAQKLIEAAALMETQPMSLQL
                     RYLQTLNQIASENNSTTVFPIPIDLFKPFLKDK"
     gene            complement(14334..15641)
                     /locus_tag="PLG98_05530"
     CDS             complement(14334..15641)
                     /locus_tag="PLG98_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOE32749.1"
                     /translation="MKQIRYALMAVFIFAGLLAWAEEKNPIFNVITVDGVITSPTAKY
                     IASGIEEAVRDNAGGLIILLDTPGGMDTAMRDIAKSILNSPVPVIVYVSPSGARAASA
                     GVIITQAAHIAAMAPGTNIGAAHPVAIGLGNSGGEKDKTMTEKIENDAAAYARSIAKT
                     RGRSEEWVEKAVRKSESITAEEALKLNVIDYVAADVNMLLAAIHQKEIQLIGGKKIIS
                     TKNAVIRDKKMGTRQGILSAISDPNISYLLLLIGLAGLYFELSTPGAILPGVIGGISL
                     LLAFFGLSTLPVSYTGILLIIFGVILFIAEIKVMSHGMLTVGGIISLVMGSLLLFDTP
                     EPALRLSLQVLIPAVVVVSIFFIVIIMLAVKAQMRKHFTGAEAMIDLEAEVTSDIEKE
                     GDVFVKGEYWKAVSDASIKKGAKVRIVKVDGMKLVVEEIKNQK"
     gene            complement(15663..16382)
                     /locus_tag="PLG98_05535"
     CDS             complement(15663..16382)
                     /locus_tag="PLG98_05535"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOE32750.1"
                     /translation="MSNITRNELQAELLHAVRGLKGQVIHNESLAVGVDWSRSSRTVV
                     AKAKTGGQSRGEMLEAIHQEMADCQLCPLGRTRKNLVFGTGNPGARIVFVGEAPGADE
                     DEQGIPFVGRAGQLLTEIIVKGMKLQRKDVYICNILKCRPPGNRNPQPDEISRCEPFL
                     KKQLQLIAPEVICALGTFAAKTLLKTDVPISVLRGRFHSYEGIKLMPTYHPAYLLRNP
                     AAKKQVWEDVKIIMKELNLPY"
     gene            complement(16393..17604)
                     /gene="coaBC"
                     /locus_tag="PLG98_05540"
     CDS             complement(16393..17604)
                     /gene="coaBC"
                     /locus_tag="PLG98_05540"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOE32751.1"
                     /translation="MLENKKIVLGITGGIAAYKAAELTRRLVKAGAHVKIIMTKSALE
                     FITPLTMQTLSQNRVYTDMFASDDEYDMAHIALAEFADAFVVAPATANIIGKIASGIG
                     DDLLSTTIMAENKPTLICPAMNDKMLANPIVQDNIRRLKKNGYAVMDSAVGELACKTE
                     GKGRLPEIDAILAEIENIMTPQDLAGVRILITAGPTEEPLDPVRFITNLSSGKMGYAL
                     ASAAQRRGARVTLVSGPTSLPLPPLEKIVTVRTAREMHKAVLQHYKSARVIIKAAAVA
                     DYRPKIVANEKIKKEGKTLFIELERNPDIIAEIGKKKGNRILVGFAMETQNLLTNARG
                     KLVKKNMDMIVANNLRQEGAGFRTDTNIITVIDRAGKTESLEKMTKIDAAGAILNRIK
                     KLISEKSKGKK"
     gene            17934..19247
                     /locus_tag="PLG98_05545"
     CDS             17934..19247
                     /locus_tag="PLG98_05545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOE32752.1"
                     /translation="MKWFRFDIRILIALVVVAALGWFIFGNYIEIGNPVIKLDSDVKA
                     IGRQKTVGITFSDSKSGLSRITAEIIQDNKGHVLADKKIPQKGVAHESLSLNIASTQL
                     ALHDGPAVLKISATDHSLLKNQTVLSQSVTIDTIPPMINLLKNINYINQGGTGFVAYQ
                     PSKPLSSTGVYINDYLSPAHAVLIDNKPSYITYFALPMTADKAATSIMIFARDEAGNE
                     TRVSVPCVIKEKKFRSDKMHLSEAFLQRKMPEFQAMVPSLQGKTLLDVFTYVNSQMRK
                     ENFSAIQKICMKSAPKELWEGEFLRMSNAAPMALFGDKRSYAYEGKVVGESVHEGVDL
                     AANARSPIEASNKGIVVFTGALGIYGNTVIIDHGLGLFSLYAHLSSIDTTVGKTVAKG
                     EKIGLSGMSGLAGGDHLHFSIMVGGKFVDPKEWWDPHWIKDNITR"
     gene            complement(19275..20141)
                     /gene="rapZ"
                     /locus_tag="PLG98_05550"
     CDS             complement(19275..20141)
                     /gene="rapZ"
                     /locus_tag="PLG98_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOE32753.1"
                     /translation="MKNLRVVIITGISGSGKSTALRALEDVGFFCVDNLPVILLPKFL
                     AITLASSPEIKRVALVMDLRQRSFLDKYSRIFASLKKKGYKIEILFLESSDESLVHRF
                     SETRRVHPLLERGIIMEGISLEREKLSSLKKMADKVIDTTSFNVHQLKDVVQRSFSVF
                     SKNKRMVINVQSFGYRYGLPVDADLVFDVRFLPNPYFIEKLKNFDGNNRNVQNYVLRN
                     RESKQFLEKIKDLMNLLTPLYEKEGKVLLNIAIGCTGGKHRSVVMANKMSAFFTSLKY
                     HVNVNHRDVNKH"
     gene            complement(20266..20709)
                     /locus_tag="PLG98_05555"
     CDS             complement(20266..20709)
                     /locus_tag="PLG98_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE32754.1"
                     /translation="MEFINAGLEAKNKDEVLAELVNVIIEGGLKLNSSSIIDILKQRE
                     NLGSTGIGEGVAIPHGKISNLNDIVVAFGRSEKGIAYDSLDGKPVHLFFLLLAPENSA
                     GQHLKILAKISKMLKDVYFRNKLIAAKSRDELYQIIIDQERTCIV"
     gene            complement(20801..21334)
                     /gene="raiA"
                     /locus_tag="PLG98_05560"
     CDS             complement(20801..21334)
                     /gene="raiA"
                     /locus_tag="PLG98_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOE32755.1"
                     /translation="MMKISVTFRNGEGENWQKNYAEEKMQKLKKYLDAPAEAHVVVSM
                     EKFRNVTEINLNSNGWNINAKEEAKDMRVALDRCVEKIEKQLKKQREKIRQHKPKTIR
                     RAQEKPLNEEEIEEPTASKIMETRKVILKPMSFDEAIMEIEETNDRFVLYRDTSSENV
                     SMIYRGDDGRYTLIETK"
     gene            complement(21595..23079)
                     /gene="rpoN"
                     /locus_tag="PLG98_05565"
     CDS             complement(21595..23079)
                     /gene="rpoN"
                     /locus_tag="PLG98_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOE32756.1"
                     /translation="MAFELKQHLKLSQQLIMTPQLQQAIKLLQLSRQELVEAINQEME
                     ENPLLEEISTDENSDEALIGEFENDGLPVERDTIKAVEHTEELNVEKGAGTDEFDWAS
                     YLEDYGPVGMTYGGKDGEETSWDNVLTEGQTLSKHLTWQMKLSSFSEDEERVGSQIIG
                     NLDPNGYLCATAQEIAQLENVSEELVERVLQKVQEFDPPGIAARNLQECLLIQARMLG
                     VKNRIIEVIIRDFLKELELKNYAQIAHKLKVPLKEVEMAVLLISEMNPKPGSIYSEDK
                     AQPIIPDVYVVKTGDEYKIILNDDGLPRLRISNFYREVMAGISSHSHEEAENGKKYIK
                     DKVQSATWLIKSIQQRQNTIYKVAESIVKFQKEFFDKGIDYLRPLILRDVANDIEMHE
                     STISRVVNNKYMHSPQGIFELKYFFGSSIRTTTQGTIASKSVQEEIRQLISSEPPRKP
                     YSDCEMVQLLKAKGIHIARRTVAKYREMMGILPSSKRKKYFKAI"
     gene            complement(23103..23825)
                     /gene="lptB"
                     /locus_tag="PLG98_05570"
     CDS             complement(23103..23825)
                     /gene="lptB"
                     /locus_tag="PLG98_05570"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOE32757.1"
                     /translation="MSDLSAKGLTKIYNKRKVVNQIDLLIAPGEVVGLLGPNGAGKTT
                     TFYMIVGLVKPDAGEIYLDGENITGNPMYRRARKGLNYLPQEPSIFRKLTVEENILAI
                     LETLDLSEAERQERLRELLKELDLTALAKNYAYSLSGGERRRVEITRALVTSPKYILL
                     DEPFAGIDPLAVADIQKIIEKLKAKGIGIIISDHNVRETLSCCDRAYIVNNGEILVEG
                     PPDTIAQSELARKFYFGDSFNL"
     gene            complement(23835..24398)
                     /gene="lptA"
                     /locus_tag="PLG98_05575"
     CDS             complement(23835..24398)
                     /gene="lptA"
                     /locus_tag="PLG98_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOE32758.1"
                     /translation="MIRKHTYIFLMLFFFFCYVEICPGKNTLTARTDKPIQITAARME
                     AFKNRSQIVFSGNATVVQGDSVLKAEKLILHYRQQPKTEKAQKARDNQAGDLEKIEAR
                     GNVSVVQGTRKATADEAVYVQAENKIIMTGNAELKEGKSSIRGERVIILLDEDKGIVE
                     GGERMRVKAVIYPQERRQTENQSTMKN"
     gene            complement(24406..24963)
                     /gene="lptC"
                     /locus_tag="PLG98_05580"
     CDS             complement(24406..24963)
                     /gene="lptC"
                     /locus_tag="PLG98_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOE32759.1"
                     /translation="MIKNRKKTIVFFSVVTVFLAVFLAAFLIFRDSINKPKTLLKALQ
                     DNVDLQIKGFVYTEVGENKGKWEVKAASASYDKKQNLAVLEQVRVTVITADGKKYEMK
                     ADKGRIFTDKKNMNISGNVVITSDDGDNFFTEYVQYSDEERKFFTDAPVRMESRRIRI
                     TGQGLAIFLNSGELSIPSMVKANIH"
     gene            complement(25095..25622)
                     /locus_tag="PLG98_05585"
     CDS             complement(25095..25622)
                     /locus_tag="PLG98_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOE32760.1"
                     /translation="MKKNLKEKLKKIKLLMLDVDGIMTDGRIIMNDEGCETKHFNVRD
                     GHGLKILQRFNIKVAIITGRQSKVVSHRARDLDITDVYQKVYNKKEVFEKILKKYKLS
                     PDETAFMGDDIVDIPVLNRVGFSATVADASDLVKKQVDYITGQKGGKGAVREVCEMIL
                     QAQGKWGEIAARYEF"
     gene            complement(25645..26484)
                     /gene="kdsA"
                     /locus_tag="PLG98_05590"
     CDS             complement(25645..26484)
                     /gene="kdsA"
                     /locus_tag="PLG98_05590"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOE32761.1"
                     /translation="MKKIKMGNVTIGDNKKFILIAGPCVLEDEKKAMAIALYLKKLTE
                     RLKMPFIFKASYDKANRTSIKSFRGPGLKKGLAILQRIREKLGVPVLSDVHRFEEIDP
                     ASKVLDVMQVPAFLCRQTDFVTEMAKKARVINVKKGQFLAPWDVANIIEKARAAGNDN
                     IMITERGATFGYNNLVFDVRSLPIMRKMGYPVIFDATHSVQLPGGRGASSGGQRDMVT
                     YLARAAVAAGVDGVFFEVYPDPEKALCDGANSLYLDSLEDLLVTLMKIDALVKTPSNG
                     KNQ"
     gene            complement(26487..28505)
                     /locus_tag="PLG98_05595"
     CDS             complement(26487..28505)
                     /locus_tag="PLG98_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32762.1"
                     /translation="MIHQDRQDRRPKLNGRLPVKTLSIAAAVVVCIAVVALLFYQGFF
                     LSLAFQMSRIKNTFSYYVLKEKPQFYYLDVEKNGQDMRIYRNDVLEITYRDEFVVKAV
                     ASDDLSGKYTYVRFEGLSSRSSDLGVLMKGIDFVNKLIRTGGMSGRSGTVNNYQILVH
                     YLNDTIASVPLKIVITPQDWFRFAKESSNVEQQIDYLKKAIELNQNDVRVRKILAGIY
                     AHHGRTDDAKKLYQQVLTIQSDDTTAMLELVKIHIKQKEFGAAAKLAGDLIKINPKET
                     EGHLALGLALEEKKAWSKAAQHYREAVRLEPHNDAARFRLADAYKNGNMMSNAIDEYQ
                     YIVKHSREPEEALFALGDIYLKLKKTDEAIKCYREILRRQPKNPVAHANLAVAYAGSG
                     KAQEELQSLKKAAALNPNEPTIRFNLGAVYERKKMYGDAAREYRHVLKINPSDTDALE
                     RLADLSLKNKQYSQAANYYERLKTKSSRKASVYANLGFAYGELKKYQAAASNYEKAIQ
                     LGAKEPVLRANLADTYEKMGMKKKAIAVYEKMPASSKDASTALADLYLKEKNYQKAIA
                     IYQKLAKQEPKKAGSYANLGFAYAAAGNYDQAIKHYNTALKFDSDDADIYDNLGEAYE
                     KKGLYQKALETYKKAHALNPESPKAAQRIPRLNIQLLQERKRQNTGSE"
     gene            complement(28561..29451)
                     /gene="xerD"
                     /locus_tag="PLG98_05600"
     CDS             complement(28561..29451)
                     /gene="xerD"
                     /locus_tag="PLG98_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOE32763.1"
                     /translation="MRALLMKYLDYLFIERGMAQNTLESYSRDLHRYLAFVQTNRKIS
                     DIDNVTPEIVVDYLTKLREEGLAANSMNRALAALRGFYKYLLREKVIAQNPLAHIELA
                     KVWMKLPETISKEEMKTILAQPGNRNNAAIRNTAMLELLYATGIRVSELINLTMNSIN
                     WQVGFLIVMGKGSKERIVPIGRIAYDCVRRYVDEIRPKFMQKKSTDVLFLSRFGKKFT
                     RQGFWKIVVACARQAGLQKKVYPHTFRHSFASHLLEGGADMRTVQVMLGHSDIATTQI
                     YTHITRERLKEVHRKFHPRA"
     gene            29568..30008
                     /locus_tag="PLG98_05605"
     CDS             29568..30008
                     /locus_tag="PLG98_05605"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOE32764.1"
                     /translation="MKKIVIASDHGAVALKSEIIAFLKTKQCELKDLGVNSDDPVDYP
                     DIAVLACTEFKRGGYDFGILLCGTGIGVSMTANKIKGIRCALIHDLFTAEMAKAHNDA
                     NFIAFGGRVKYNVPVTKMIEKFMETEFAGERHCRRVGKIMDAEK"
     gene            30041..30901
                     /locus_tag="PLG98_05610"
     CDS             30041..30901
                     /locus_tag="PLG98_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE32765.1"
                     /translation="MSDILDKKKIRKVFRNVQKHLSIAQLIRRFSTNKEDIRKAALNG
                     VNLSRCRSVLELGCAFGAFTEALRNKLHDGATITGVDIISEYKPFFLDACRRAGYEGT
                     FLSSGIEKIKKIPSGFFDLVICSYALYFFPEIIPEIARIMKPSGFFITITHSSNDMRE
                     IVDIVKRILGRHHASGTNRSLPIEIIFDQFSAENGESLLQPFFKTIEAIDFKNTLVFQ
                     SQEIRYFMDYFQFKKSFFLSEQDANNQTIIRQLFKELQETLSKNQIVSMCKDDRIFIC
                     SQPSTGKDSP"
     gene            30898..31896
                     /locus_tag="PLG98_05615"
     CDS             30898..31896
                     /locus_tag="PLG98_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOE32766.1"
                     /translation="MKKQRKHCLYCGGKTVKKYEDNVRRDFCPVCNSYFYENPLPVVS
                     SILEDSRQILLVKRGKRPSKGLWCLPTGFAEAGESIEEAALRELEEESGIKGKIIKLL
                     DVDSYKSRFYGDLLFLTFVVQQTGGKLCAGDDCSQARFWPVHKLPPLAFRSNKRALDA
                     YIKSRKDYWAIFDSIEHADKKSLPPAVAKNSLTRRLVNVIIKNKEKIIEQWLEDVIVN
                     PSTTEYHKVERTVLYNICDKILSQLTLWLGGLHDVDKIRNFYTKLGKERRISGFKISG
                     ALSALSLIRKHIWTFALAQDALQKNLELLMTFELQRRMTVFFDLATFYLTKGHEEK"
     gene            32061..>32376
                     /locus_tag="PLG98_05620"
     CDS             32061..>32376
                     /locus_tag="PLG98_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE32767.1"
                     /translation="MEFKDVIKQRRAVNFFDPTKNVTDAQLKQIIETAALAPSGFNLQ
                     PWRVIVLRDKEKKEMLRKVAMNQPKITEAPVVLIVLADREGWKKGNPGFEKDFVESVK
                     EGI"
BASE COUNT         8005 a         7995 c         7079 g         9097 t
ORIGIN      
        1 ccttccttta cggcattgcc cagaacaata tcttcgccgc tgtgatagac ataattgaca
       61 atggagtgac agatatcggg gcaatctttc aggggcaggg attgcatcac ctggctctca
      121 cctttgtcta catcctgact ggcttcaatg gtcagatcgc catccgattc caaaagcaaa
      181 taaccgcgtt gcgctccggc attcgtaatg gactcatgca tgattttctg cagcagacgg
      241 tcgagcatga tttcactggc aatgatctgg gagactctca tggctgttga cagatcaagc
      301 atttttgtgc tgccggtggt ttcggttgca ccgctgatgg tcatcgatcc tgaaggaccg
      361 ctcaccgtga cggttcccgt cccccattgt ttctgttcca gtgggacaag cgacggatat
      421 ttttcctcca gatctctggc tttctccgaa gcgccccagc ccaggtagcc tttatgggct
      481 ttctgcatga aaaatcccgc ttcatccctg catctgtgat caagatacaa aagcgccaga
      541 cgttcgcagg caatggcctc ttcctgcctg tatccatttt cccgggcgcc ttcaatcgct
      601 ttatgataga gtttctgcgc ctcccggaag cgccctttgt ttttcatcat ttccgccgcc
      661 acaagatcat atttatgctg aaaattttcc ggacaagctt tggcccatat gagcattttc
      721 atctgattag ccctcaacga cattttatac cgggcttttg tcatcggatc cgcctcggga
      781 taagatgccg tcatagtcaa tgacgaataa agatagaatt ccgggatgtg aagattcccc
      841 agcgccgtct ttttttgaac aatccttcgc atctcgggaa gaagatccct gcacgccgaa
      901 taattgccga acagataatt gattctcaac cggaccaacg agaagtaaaa agtgcccagc
      961 aaattatttt cagacttgta ataaccggcc tgctcctttg catcaaagcc gtccccgttc
     1021 aaacttcctc tgctttcggt ccggcctttc agacacaggc acatgcgcgt attttccatg
     1081 tagttgcgcg cgataaaagg atctttgccg ccgagctgaa aatccctgta ttttccgtat
     1141 tcttccagaa tatcgtcgag gttgtccccc agcatgattc gggtcatgcc gatcagatta
     1201 acattatgac cgctgaaaat aagatcgccg ttttcaatgc cgtttttata agcgtttctg
     1261 aaataatcaa ggtctttgcg ggcgtgtttc ttccagtgct ggataaacat cggaaatgcg
     1321 aattctatcc tgcatcgatt ctttgtatcg gcgatttttt cgttggtttt taatgccgct
     1381 accccgaacc ggtatccgtg ctgatagaag cccagnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnncgttg gtttttaatg ccgctacccc gaaccggtat ccgtgctgat
     1561 agaagcccag agaggatccc acaatagacc ccagggcgat ataggcaaat ggcgaatatt
     1621 caaaattgat ctcgtagtcc agcagcctgt aaacaccgtt gatggcgata aaagcaaaca
     1681 gattgggatt agcgtaaaag gccacagttc cggtatgaat ggccagatac gcgtacgaaa
     1741 gcatttccca gaggttgatt tccggagatg ccagtgattt tccgggacgc accctcagat
     1801 actccgcggc catctgtttt gcttcatcca acgtattggg catgggaatg tattgcaaat
     1861 caatcaaatc ttctattttc cgacgaccga actttaagcg gagtttcagc agttcccagc
     1921 ccacccggac atcgctgacg ttatccggca gatggatgcc gagcattttc attccttcca
     1981 tgccgacatg cagtgccgca tcgtagtttc cctgtgttgt atatagaatg atcatcagcg
     2041 tgtaaacatt ggccttgtca atattcgacc gggcattatt cacaaccgca tgaaatattt
     2101 tttcggcttc gctgaaattg aggttgagat attcacattc aatggcttcc ttgtatatgg
     2161 cataggtcag ttcgtaatgg cttttccagg cgtattcggg caaaagcccc atggccgttt
     2221 tcaaataaaa tgaagcagaa ccgtacgctg tggatttttt cgatttctgc gccgcctgca
     2281 gattcaatct ggccagcatg atttcttcat ctgttccgct gacgcacctg atgcctctgt
     2341 tgagctgatc gacaatatag aatatttttt catgaagatt cttttcatcg gtttttttga
     2401 gaacattgcc gccgatgcga tagtgcatcg cgcttttttc ctcctcggga atcatggaat
     2461 aggcggcttc ctgaatccgg tcatgcatga atttatacat atttccgtga agactgatca
     2521 tatcttcctg gattgccgaa gtcagatcaa acagtgtttc gtctatggat tttccggaaa
     2581 caaccgacaa caactccaga tcaaatcggt tgccgataca ggcgcagatt tttaatattt
     2641 cccgggtgtt cttcggaaaa tgcgatattt tttccgccat gaattcaatc acgttttcgg
     2701 taacctgcat ctggcgtatt ttgtcgatat cccattccca tccggtggaa ggattcagtt
     2761 ggagcaggcg attatcgtaa atatttttta gaaactgaat gacgaaaaag ggattgccgg
     2821 cagttttcct gttgatgagt tcggcaagcg gcattgattt ttccgcatcg cacaacagaa
     2881 cattcataat gatcatgttg atgccggtca gattcagagg tcccaggaca atgctgttta
     2941 ttttcttgcc ttttttgcgg atatgatcca gcgcgagcgt cagcggatgc gcatcgttcg
     3001 tttcattatc gcgatacgcc ccgattagaa acannnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnaattttt tcttcgcttt cagaaatgat ttgacggatg agtccctgga
     3181 atgactggat gatggcgctg tacggaacat ccttgcgaaa ctggtcatat ttgccgaaaa
     3241 taaaataccc ttttctggca ataatcggtt tgtaaatctc attgaccagc gccgattttc
     3301 cgatgcccgg ctggccggtc accagcatga actcgcttaa tcccttgcag gttctttcaa
     3361 agcacctcat cagttcaatc cgttcattgt cacgatccac aagggtgcgc ggaatattaa
     3421 accggatgga aatatctttg gtggcaatcg gaaaatcgtc aacctgtcct ttctggttga
     3481 gttgcctgag gcattcatcc agatccgcca tgattcccag acaattctga tatctctcct
     3541 cgggattctt cgacaacatt cttaatataa tggaagacag caccggcggt atggacgggt
     3601 ttagatggtc cggcggcaac ggctgtctcg cgatatgcga atgaatcaat tccataggat
     3661 ctttggattt aaacggaacc tcgccggtca gcatttcata aaaggtgaca cccagcgaat
     3721 agatgtccgt tcgataatcc acgccccggt tcatcctgcc cgtctgctcc ggcgacatgt
     3781 aggacagggt tccttcgatc acgtccggat tgtacagttc gtcattggca tgagtcagaa
     3841 cagcggaaat accgaagtcc gcaactttga cctcatccct ttccggatta attaaaatat
     3901 tatcgggctt gatatccaga tgaataatat tgtttttatg aatgagcccc agggtcccgg
     3961 agaccttgga ggaaatccgc agtaatgatt gcaggtcgag ttctttatct tttaaagaat
     4021 ccttcaggga aatgcccccg aaatcttctt caataatggc atactggtta tcatagtcca
     4081 ccagatcaaa tattttaaca atgttatcga catcaagaga tttgacgaga ttatattcct
     4141 gtttgaaacg tgcaatttct gatgacgtcg gattgatggt ttttagaagc tttatgatta
     4201 agggcaaacc ttctgtctct ttatagcctc gaaaaatgac agaatttctg gtttcgtgta
     4261 tcttttcact caggatataa ttggcaaatt tctccataaa gttcagacga atttaataaa
     4321 atggtttgta ttcaggaaat aattgtttaa tttataacac ttttctgtct tttgatcaag
     4381 cattacctat cattatatta gaaggatctt cagccctcca cggtaaatgt atagcgcgca
     4441 gtaatcctgt aacgaccggg actgtacctg taaaacgccc gcgcttcgta tcgtcccggt
     4501 cccggagaac gaaacgtcaa gacaccctgt ccacgccggg gaatgtattg gtaatctccc
     4561 gcatcggtgt ttcttgagcc ggcaggcacg atgcatatcc aatccctgga atgtccgggc
     4621 gcgttataat aatgtacctg aattttctcg ccgcttcgat acgtgtccct gtcagtctgg
     4681 ataatttgct tagatgagtc tgcctctgtt ttgctttttc caaaaatact actgccagcc
     4741 tttcccactt cgtctgaatc atgactttcc agaccggctt gtgatgttgt ctttgaagaa
     4801 tcgccggata aagtgaaata agcgatcagt ccaatagcca tgatcagcaa aatagcaaag
     4861 attggcccca ggactttgat gaaaggaatt ttatttatca aacccgtcgt ttctgttttt
     4921 gatttgggct taaaaatatt caatttaaaa ttccagccgt gtttggcggc aatatctttc
     4981 acaacatcct tgagctttcc gtattcaaaa ccatacttac cgccgaacaa taattgtcca
     5041 tgaattttct ttccgacatc gattcccttt tgaacggtct tttctgcgaa aacgcctgcc
     5101 gtcattccgg cggaactttc taccatcttt tcccagaacg ttatttgttt cgttagcggg
     5161 tcaagtatcg ctaaagcggt gtattttatc ttcacgtgtt gaaccgcaga tcgagctccc
     5221 gtttcggaag aaaattcaaa ctgccccgca gaggattccc ggacagacaa aaattcgttg
     5281 tttaacgcca aaagctgtgt tgccacgggt gaggattccg acgcttgggc tttcggccct
     5341 tccagacggc ttccacagtc gctgcagaag gcgtcttcag gtttattgga tgttccacag
     5401 gaagggcatt tttttacgtt ggaatcttct gcaagacgtt gtccgcaatt cgtgcaaaaa
     5461 atggcatttg cttcctgaac ctctccgcag gcggaacatt ttttcatttt ctcaactcct
     5521 catcatctta ataatattgc tctcaatggt attttccttt atgaaactgt ttcataatac
     5581 atttttttaa tactacatcc ggctatatca actcaaattc aaaagtgctg acgacacgta
     5641 tctttttgac aggcgtgctt gtcggactgt atggagcgga ctcatcattg ccgtcactgc
     5701 cgaaaatctg gatattcccc tgattggccg aacggatttt accgacacgc gctcccgaat
     5761 cggaggcaaa ttgctgcgct gtcatacgcg cattttttgt cgcctcggca agaagcggcg
     5821 gcctcaattc attgaatttc gtgattacgt agcggggatt agccgttcct tcgtcctgac
     5881 catccagaac aacgccggat ttcagcaatt cctcggtttc acccaaagct ttaatgacga
     5941 gatcgatatt cgaggttgtt acaaccagag agctggttac gacgtaacga tatcgttcgg
     6001 ttgcctgact ttgatcatac tcccgcgcca atttatcgat ggtgcgggtc ggttgacggc
     6061 taatttcctc atccttaaat ccctgttttt tgagaaaggc aattccggca tccctgtcgg
     6121 cgctgatttt ggtttgcgca tctttgatat cctcgctcgc ccttcggaaa cgcaataccc
     6181 agatcgactg atcggccttt accacttttt ccacaagtcc tttcaccgtg acggtccgcg
     6241 tatccgattt aaaacgcgtg ccgcctttgc cgatgaagta tcctccgatc gcaatgccca
     6301 ttaaaaaaca tatcccgaca atcagcgccg ctatgattac aattcgattt tcattgtgcg
     6361 tcattttacg ataccctccc cgatattgta ttattgtaaa aaaaattcac gatgattttt
     6421 aaaatataag taacgatagc attatacgtt acttaagaaa aagagcggga aacaatactt
     6481 cataggttgt aaggttttct tatggcgcca tttttcatga acattttgcc ttccttaacg
     6541 ccttttaaaa aaggatttat tttatgatac ttttgcgcca cacatcgcgc agaacttagc
     6601 ccccggttcc agttctgtct gacattgttt acatctgctt tctaaaattg gatctttggg
     6661 atgttcgcca gacattgatg ccgtttccac tttgctgccg cagacgccac aaaactttgc
     6721 accctcgggg ataacggctc cacatgcgca tgtattcgcg gacgggacat cttcccttaa
     6781 agagtcctga gccctcttct gcacctcaac aatctcattt tgcagattct ttatctgctc
     6841 atcgatcttc gaaatctcat tgcccttatt tttaatgata tctgtatcaa tgttgttctt
     6901 caaaaacatt gcataagcaa tattgcccaa ttcctccagc gcatcctgct tcttattttg
     6961 aagagaagat atttgatttt tcagttttgt tacatcaaga aactctttgg acttgacgct
     7021 tgcctttgcg acacttttac ttatgcccag cttgaccttt tcaaataact ctgacatccg
     7081 aatctccttt ttttacagac attaatcatg gatgataaaa ttataaattg aatgatttca
     7141 cgtgtcaatg agtttaaaag taacaaatta cctcaaaaat attcaaatat tcatggtgta
     7201 atacgatcgt cattccccga aaaactcagg ccgaaccgac attttttatg tcttgaatct
     7261 tcttgacaca caaaaacatt tttttctata attacgcaca gaaaaaacat tttctaaaag
     7321 aataaacgta tgccgaatat acagtttttt gcgttagccg aaagtgtctg caaaaagctc
     7381 aatctcccgc cgattccttt tcctgtccgc aaaggccgtg atgaaataat atttaatcca
     7441 cagggtgtcg ccctcgattt gcttctggat gagctggagc agtatttaat cgaaaagccc
     7501 gaagaaaaga ttttctacaa agatgcattc tcgcggcttg ctgccatcga aggaatccgt
     7561 ctgggcgagg aaggattcca tgatcacgcc gcacactatt ttgaattggg actggccctt
     7621 gatccggcca atctagccct tcgatccaat tacgcacttt cgctccaaag tgccggccgc
     7681 aaggaagagg ccatgcgtca gtaccgcttt cttctgcagc agccggtaat ttccggtcaa
     7741 tgtcttgttt tgattccggc cgcccgtctc tttctggatt gcggtgatcc ggtcacggcg
     7801 catcagattg tcaagcactg cgcgtctttc atgccggtgg ataacgaatt ctgggatcta
     7861 tttgccgaag cccgacagcg ctgcggaatc aaacggtgga tggcaccccg ccaaaagcaa
     7921 caagaaatta tgcggcaagc aaccgcgcct gattataaaa gtgcgcccac gggcaagaag
     7981 aaaaatttct gcccggcctg cgggaatcgc atcaagcccg aagccagatt ctgtggaggt
     8041 tgcggtcgtg cgctgtaaaa atgatttttc ggttgaatgc atgaaggcga aggcatttgc
     8101 agtgcagagc gtgctatacg catttcgcgt tttcactccg gttctcattt tgttaatcat
     8161 cctattgctt tttgtttccc atgctatcgc ggacgcctct gattatcgcg ataaaggaaa
     8221 ggaaaactgg gtcctgttta catttatact caaggacgcc aaaaccggcg aacccataaa
     8281 aggccgcgtt tattatcagt ccgacctggc cgcggactgg tttcatccgg ataacaccaa
     8341 accacttttg cctccgggca tctatcgagt agtgggatgg gagagacatt actggcaatc
     8401 cgagcaaaaa ctgattgtta atccacttaa gggattgcag cagcaaattg ttattgatct
     8461 ggagccggtg tcatctccaa gagacggcaa atcgggttcg cccttgaagg ttccgccgat
     8521 tttgtcgaac ctgatcatca ccggaaacgc agcggtaaaa atgccgcata tgaatgcgtc
     8581 atggttcggg cagagcagta tggccacttt tcatgtgtat acgaatgcgt ggtcggaata
     8641 tcccggcggc atcggtggag gctcaagaaa cagcacaggc catattgatt actacttttt
     8701 ccttcatacg gaagacgctc aaaaacatta tgaacgactt cgtccgggtt tcaaggtaat
     8761 tcccaacagt tcgcaacatg tttctggccc gactccggtt tcgctcggtg atgaggcact
     8821 tcagatcagc atcgacacgg agcgtccgga tggaaagggc cttttgtatg aatcacgaaa
     8881 gtatctcatc cgcgatggtc atttcgtgat gaatatgtac atcaacagag aaatcggctt
     8941 ggatttcctg acctcgcctg aggaaattct caaacgtgtc cgtgcgctcg atgtacggca
     9001 ggtcaagcaa atgctggaac ccgagcctta tccctggcct gaaattaccc caacacccgg
     9061 ttcaggaaaa gatgttgatt ctttaggatc taaaaatgag gttgccgatt atgcctctgt
     9121 ttccccggag aaggcgctta tagctgtggc cggcgtgtcc atggtgttga ccttgggggt
     9181 attggtgcag ctttggggaa tgggtggcct tcagagtccg ggagatattc gcggagcgct
     9241 ggagggtctt ttcagttcct ccgggggtga ggtgacagag actgaatctt ctgtacctga
     9301 gaccggggct gtgcaggaag tggaggtcat cccgaaagag ccggaagcac tcgtacccga
     9361 gaccgttatt caggaatccc gtgttgtgga aacagttatc cccgatcccg ttccgtcggg
     9421 cttcgagtat cagggcaaag tctggtataa gccgccgtgg gacaagggcg gtccatactg
     9481 gatggataaa tctgattacg atgccatgcg ctccatgatg cgccggggta tggtctggtc
     9541 ggatcgctgg ggctgggtga ctccggaaga cggaaaaacc atggaagccc agcgcgtcgc
     9601 ggcctgggat gaatataaat caaacacgga taaagacatc aaagtcctta ccgacaggat
     9661 tgccgaatcg caggaaaagc ttgccgagat ccgggaaaag caggccgaac ttgaaagaat
     9721 cgaacagtta agagagcgcc tgactgagct ggaaagacag cgggaactgg acaactcatt
     9781 tgccacgcag cttagtgaca cctgggacaa ttatgtcgaa ggagtgaaca gggatttaga
     9841 cgcccttccc ggtgagttgg gcgatatggc actagatgcc gcccgcggga taagagacac
     9901 aaccggcgca ttgattacag ccgccgggga tactgtttct gatttaacca atggagacaa
     9961 ctggaaagcc gtgggcaagg cggcggtgga tacggccaaa gatctgattc tccatccgtt
    10021 ggaatcttct gttaaggttg gtgatgtcgt caaagacgcc gggagcatag ccatcaaggc
    10081 cgcggacgtt gccggaaatg tggcggttgc cattgtttcc gatccgtttg cctttgcgga
    10141 atcggtcgtg ggcattgaca actggaaaaa agttcttgat cctaatgtgc cagtgggaga
    10201 gcgtgtcagc cgcgcgttat acggagcagt agacactgct ttgaactttg cctcgggtgg
    10261 aaccaaaacg gcggctgccg gcgttgaagc cggtatggat gcgctcaaaa cagccgatac
    10321 ggtatcggat gcagccaaag ccgcaaatgc agcagacatg gcggcagacg cggcacgcat
    10381 taccccgaag ctcgatgacc cggcccgtgt gcaggtctgg gaagtgggcc gccaggcagg
    10441 ccagcagaaa gcggacaaac tggccgaagc gatcaaaagc ggcaagccct ctgaaatcaa
    10501 gcaagctctt gtggaatgcc agaaagacaa gcacgccatc ggagcgctca atagggcgga
    10561 tgataccgta aaacatgcgt ataatcagca aatgaaacag cttatcacgg atgatgtgga
    10621 taaggtggta aaggaaaaaa tcgctcagcg ctatgggctg gatcccaaag atgtggagat
    10681 tgtcaagccc acgaatccca cgtccaaacc gaaggttggc agcgatcttg attttaccgt
    10741 caaagtaaaa gcgaaacccg gtgagatcgt gcccgatcct aataagccgg gaagcttcat
    10801 tcatgtgaag ccgggggata atctgaccaa ggatgttccc ctcaaagacg cccaggaaat
    10861 ttataatcgg gaactctaca gcaaggtaac gggagagccg attccgacgg atcctgaaaa
    10921 gtttaaagcc tttcaggccg aagccgacaa actcgcgcat gagatggacc atgtagcggg
    10981 acatcgcgtg ggagcggaat cctacggcgc ctcgcagcag gatttggaag tcattctgca
    11041 gaaatccggc ggccggttta cggatccgga acaggcggga atggtagctt cctacaagtc
    11101 cacgcatctt taccaggaag cggaaagcat cacaaaactg gatcccggta aagccgaaca
    11161 gcttgtcgcc gacggcatgc ggcagaccac taaacaatac agcaatctga tcgaaacacg
    11221 ggtggccgcg ctgcagaacc agggcattga ggttgccgtg gatccgcgcc tgacgaaggc
    11281 ggtggacatt tttaaacagg tggaaaccaa aggcctgcct ccggcaaaag ccgaagcgtt
    11341 attgaaaagc cagttgaata tgtccaagga cgatgtagcc aatatgctcg gcgcgcagct
    11401 ggctaaactg cacagggtga aagcttaatc atgaagatga gggaatttta aattatgtgg
    11461 gctgttttcc tgaaaattgt ttcgtacttt ttcctgctcc tgtttgtgct gggcgtggtg
    11521 ttgatgcttt tgacctggcg caaggaaaaa ttcgtcaagc cgaagtggat tcttttcggg
    11581 cagattgtgg cgtttatggc acttgtggtc tttacaatgt tgtcccggaa tccactggga
    11641 ttctggagct ggttgctcat ctttctggcc gggctgtgtg gcggatattt ttacggccga
    11701 acggtgaaag tgaaaaagtc tgcacgcgga atcatgatga actacaccct gccctatgtc
    11761 atcacgtggg gcgtgctttt attcctcacg cagtttttga cgatcagcac gggtcgtatt
    11821 cctgtgattg tgctgggcct gtgtgtgctc aatactggat taaatctggc gatgaatggc
    11881 caagtcgtct ggaattataa ccggatgaaa aaaacctgac ccctaattac gcatcaaaag
    11941 aaggcttttg tttgactttg tcattgatca gaattgcata tgctatttgc cggaagtttc
    12001 cctgatcgct tctgtaaatc cccgcatgga attgacaatt gttttgtcat caacgcagta
    12061 ggcgatcctg aaatatccgg cagcgccgaa accgcttccc ggaacaacca ggatattttt
    12121 ctgcaaaaga attttagtga atgcaacatc atccggtagc gggcttttga caaacagata
    12181 aaaggcccct tgcggtttgg caaatttata accggctttt gacaaccctt tgcacagcag
    12241 atcgcgcttg ttctgataca cgtttacatc caccgctgca ttttgcagtt tcacgacaat
    12301 tctctgcatc aatgccggtg cgtgaacaaa tcccagaatc cgggtgcata aaatcagagc
    12361 actgattaaa tcccggcaat cgtcaatttc cggattgacg gcaatatagc cgattctttc
    12421 ccccggcaga gaaagcgtct tggaatagga acaggcaacg atactgttcg tgtaggattt
    12481 cagcacgctg gggactatta cccggtcata aacgatttca ttatagggtt cgtcggaaac
    12541 caggtaaatg gttttatgaa acgttttgcc ctttttcttc aaaagggcgg caagcgcctg
    12601 aatgtttttt gaactgtaaa cctttcccgt cgggttattc ggtgaattga ttaacaccgc
    12661 ttttgtcttt ttgttgatgg cctttgagat cgcctcgatg tccagagaaa aatcttttct
    12721 ggttctgacg aattttgtta cgcctccgta gttgtcgatg taaaatggat attcgacgaa
    12781 ataaggcatg gggacgatga cttcatcatt gggatccagt aatgatttga aaataacatt
    12841 caacgcaccg gctgcgccgc aggacatgac aatatggtcg gcgcttaaat tcagatcgca
    12901 ggttttactg acctgcttgg caatagcctg ccgtgtatcc gcaaaaccgg cgttcggcgc
    12961 gtatccgtgc ttgaggggca atggctttgc ggccagacga atcaattccc tcttgaagac
    13021 tgccggaggc tcgacgttgg gattccccag gctgaaatca aagactttat cggcgccgta
    13081 ttttcttttc atcacggcgc cttcttcaaa catctttcta atcatcgaag aattcgaaat
    13141 cgatgattta attttttttg caattgacaa cgaatgtatt atcctttctt tcttctgagg
    13201 agcacaaaaa tgcccagcaa aatgataata ccgggcaaca catatttcga atactgggaa
    13261 acatcgaatc cgtagaactc ataaaagaga aaccccgtcc cggcaagcgt tataatccat
    13321 ccgccgagat ctctgaactt ttgtattagc gtgcagatac cgatgacaat caacaaaacc
    13381 ggccaggtct ggccgaacgg gaaacttccg gacttgctca gataaatcaa aattcccaac
    13441 gtaattaaaa ccgttccgcc cgtaatcaca ttctgtttct tatcgctcat gcaagaatct
    13501 ccttttcgtg tattggttat ttaaagcgtc tctgcttttt tccggaaatt atttatcttt
    13561 caaaaaaggt ttaaacaaat caatcggtat cggaaatacc gttgtggaat tattttccga
    13621 agctatctga ttcaatgtct gcaaatagcg aagctgtaag gacattggct gggtttccat
    13681 caaagcagcc gcttcgatca gcttctgcgc tgcttgaaat tcgccttcgg cattgatgac
    13741 cttggctctt cgttctcttt ccgcttccgc ctgctttgcc atagaccgtt tcatctcttc
    13801 cgggaggtca atatgtttga cttctaccag cgacacctta atgccccagg gttcagtgct
    13861 gcggtcgaga atttcctgaa gattcatatt aattttttcc cgttcggaaa gtatttcatc
    13921 gagttccatc tggccgcaga cacttctcaa ggtggtttga gcaagctgcg atgtggcgta
    13981 aagataattt tccacattcg tcacggcgga attggcatcc atcacccgga aataaacaac
    14041 cgcactgact ttaatggaaa cgttgtcctt cgtaataacg tcctgcggag gaacatccat
    14101 ggtaatggtc cgcatatcca cccggaccat tttgtcaatg ccgggaataa tgataatcaa
    14161 gcccggcccc ttggcatccc ggattcgacc gagtcggaaa ataaccgccc tttcgtattc
    14221 gctcaatatt ttgattgcgg agaccaaaaa cattacgacc agaacgatca cgaccagcgt
    14281 cggcatagat aaagttccca acatatgtat tcctcctctt tttattctaa tccttatttt
    14341 tgatttttta tttcttcaac gaccagtttc atgccgtcaa ccttgacgat tctcaccttg
    14401 gcgccttttt tgatggacgc gtcactgacg gctttccagt attctccttt gacaaaaaca
    14461 tcgccctctt tttcgatgtc gctggttact tcggcttcca gatcaatcat ggcttccgcg
    14521 ccggtaaaat gttttctcat ctgcgccttc actgccagca taatgatgac gataaagaaa
    14581 atagagacaa ccacaacggc cggtatcaat acctgcaagg aaagacgcaa ggccggctcc
    14641 ggcgtatcga acagcaataa cgatcccata accaatgaaa taatccctcc taccgtcagc
    14701 atgccgtgac tcatgacttt aatttccgcg ataaataaaa tgacgccgaa tattatcagc
    14761 aaaattcccg tataacttac cggcagcgtg gacaagccga aaaacgcaag caaaagcgaa
    14821 atgccgccga tcacgcccgg cagaatcgcg ccgggagtgg acagttcgaa gtaaagcccc
    14881 gccagtccga tcagtaatag aagatatgat atattgggat ccgaaatggc ggaaagaata
    14941 ccctgacgcg tccccatttt tttatcgcga atcaccgcgt tttttgttga tattattttt
    15001 ttgccgccga tcaactgaat ctctttctgg tggatggcag ccagtaacat gttaacatcc
    15061 gccgcaacat aatcgatgac attcagcttc aaggcttcct ccgccgtgat cgattcgctt
    15121 ttacgcaccg ctttttcgac ccattcttcc gaccggcctc ttgttttggc aatgcttctg
    15181 gcataggcgg cggcatcatt ttcaattttt tccgtcatgg ttttatcttt ttctccgccc
    15241 gaattcccga gaccgattgc cacaggatgc gcggcgccga tattggttcc cggcgccatc
    15301 gctgcaatgt gagccgcctg ggtgatgatg acgcccgcag aagcggcccg cgctccggac
    15361 ggcgatacat aaacgatcac gggtaccggt gaattcagaa tgctcttggc gatatcgcgc
    15421 atcgccgtat ccatgccccc cggcgtatcc agcagaatga tgagcccccc ggcattatcc
    15481 cgaacggctt cctcgatgcc tgatgcaatg tatttcgcgg tcggtgacgt aatgacacca
    15541 tccaccgtga taacattgaa aataggattt ttttcctctg cccacgccag aagtcctgcg
    15601 aaaatgaaaa cagccattaa tgcatatcgg atttgcttca ttgccttacc tcgtttgatg
    15661 gctcaatacg gaaggtttaa ttccttcatg atgattttta catcttccca tacctgcttc
    15721 ttcgccgccg gattgcgcag aagataggcc ggatgatagg tcggcattaa ttttattcct
    15781 tcataggaat gaaaccttcc acgcagcact gaaatgggaa catctgtttt tagcagcgtc
    15841 ttggcggcaa acgtccccaa tgcacaaatc acttccggtg ctatcaactg gagttgtttc
    15901 ttgagaaaag gttcgcacct gctgatctca tccggctgcg ggttgcggtt tcccggagga
    15961 cggcatttga ggatattgca gatataaaca tcctttctct gaagcttcat gcctttaaca
    16021 ataatttcgg tcaaaagctg gcctgctctg cccacaaaag ggatgccctg ctcatcttca
    16081 tcagcgccgg gagcttcacc gacaaaaacg atccgcgctc cgggattgcc tgttccaaaa
    16141 acaagatttt tccgggttct gcccagcgga cagagctgac aatcggccat ctcttggtgt
    16201 atggcttcca gcatttcccc tctggattgc cctccggtct tggctttagc aacaacggtc
    16261 cgtgatgatc tgctccaatc cacgccaacc gccagactct cgttatggat gacctgtcct
    16321 ttcagccccc tgaccgcatg gagtaattcg gcttgtaatt cgtttctggt tatgttcgac
    16381 atcgttcgcc tgttatttct tgcctttgct tttttcagaa atcaattttt taatacgatt
    16441 cagaatcgca ccggccgcat cgatttttgt cattttctcc agcgactccg tcttaccggc
    16501 gcggtcgatg acggtaataa tgttggtgtc ggtgcggaaa ccggcaccct cctgacgcag
    16561 attattggca acgatcatgt ccatgttttt cttgaccagt tttccccggg cattggttaa
    16621 aagattctgc gtttccatgg caaaaccgac cagaatccgg tttccctttt ttttgccgat
    16681 ttccgcgatg atatctggat ttctttccag ttctataaat agcgttttcc cttccttttt
    16741 tattttttca ttggccacga ttttcggacg ataatcggct acggcggcgg ccttgataat
    16801 cacccttgca cttttataat gctgcagaac agctttgtgc atttctctgg ccgtacgcac
    16861 cgtgacgatt ttttccaacg gcggcagcgg cagtgacgtg gggccgctga ccagcgtcac
    16921 gcgggctccc cgtctttgcg cagcggaggc cagagcgtaa cccatttttc ccgacgacaa
    16981 attcgtgatg aaacgcaccg ggtcgagcgg ctcttctgta ggaccggcag tgatcagaat
    17041 tctgacgccc gccaagtcct gcggcgtcat aatgttttcg atttcagcaa ggatcgcgtc
    17101 aatctccggc aagcgtccct tgccctctgt tttacaggcc agttcgccta cggcgctgtc
    17161 catgactgcg taaccgtttt ttttcagtct ccggatattg tcctgaacaa tcggattggc
    17221 cagcattttg tcgttcatcg ccggacaaat cagcgtcggt ttgttctcgg ccatgatcgt
    17281 ggtggaaagc aaatcgtcgc cgatgccgga agcaattttt ccgataatgt ttgccgttgc
    17341 cggagcaacc acaaacgcgt cggcaaattc ggccagcgcg atatgtgcca tgtcatattc
    17401 gtcatcagag gcaaacatat ccgtgtaaac tctgttttgc gaaagcgtct gcatggtaag
    17461 aggcgtaatg aattccagag cgctttttgt catgatgatt tttacatggg cacctgcttt
    17521 cacaagccgg cgggtcaatt ccgccgcttt gtaagccgca atgcctccgg tgattccaag
    17581 aacaattttc ttattttcca gcatatggtt cgacttccag aaatttttga ttaatttttt
    17641 tgaaaacagc tcgtggcgct tggccgttgt ctatgaatat aacaatgctt tttcacaata
    17701 tcaaggtata attattattg atatcttgaa aattttataa ggaaatggtc ccatgtagct
    17761 taattcttca ctgtgcttgc gccgctccgc ttcgctgcgg ggataattcc acttacgctt
    17821 cgagcttcat tacattcgct cttagcacgt ggcattaatt tcccttgcaa atcctccgga
    17881 atgctattat aaaccgagca attctattta catcacaacg ttaggagatt attatgaaat
    17941 ggttccgttt tgatatcaga attctcatcg cgctcgtggt cgtcgccgcc ctgggctggt
    18001 ttatcttcgg taattatatc gaaataggaa atcctgtgat taaattagac agtgatgtga
    18061 aagccatcgg cagacagaaa actgtcggta ttacgttttc cgattccaaa agcggtcttt
    18121 cccggatcac ggcggagatc attcaggaca acaaaggtca cgttctggcc gataaaaaaa
    18181 tcccgcaaaa gggcgtggcc catgaatccc tgagtctgaa tatcgccagc actcaactgg
    18241 cgctccatga cggccccgcg gttttgaaaa tatcggcgac ggatcattcg cttttgaaga
    18301 atcagacggt gctgtcgcag tccgtcacaa tcgatacgat tccaccgatg atcaaccttc
    18361 taaagaatat caattacatc aatcagggag gcacgggctt tgtcgcgtat cagccttcaa
    18421 aacccctctc atcgacgggc gtttatatca atgattactt gtcgccggcg catgccgtct
    18481 tgatcgacaa taaaccttcg tacatcacgt atttcgcgct gccgatgact gccgataaag
    18541 cagctacaag cattatgatc tttgcccggg acgaagccgg caatgaaacc cgtgtgtccg
    18601 ttccctgcgt gattaaggaa aagaaattcc gttccgacaa aatgcatctt tcggaagcgt
    18661 tcctccagcg taaaatgccg gaattccagg ccatggttcc ttcgcttcag ggcaaaacgc
    18721 ttctggatgt attcacctat gtcaattccc agatgcgaaa agaaaacttt tccgccatac
    18781 agaaaatctg catgaaatcg gctcccaaag aactctggga aggcgaattc ctgcgcatga
    18841 gcaatgccgc tcctatggcc ctgttcggcg ataagcgcag ttatgcgtat gaaggaaaag
    18901 tggtgggtga atccgttcat gaaggcgtgg atcttgctgc caacgcgcgc agccccattg
    18961 aggcatccaa taaagggatt gttgtcttca ccggagctct gggcatttac ggcaatacgg
    19021 ttattatcga ccacggtctg ggattattca gtctttacgc ccatctctca tccatcgaca
    19081 cgaccgtggg caaaaccgtg gcaaaaggcg aaaaaattgg tctttccggc atgtccggac
    19141 tggcgggggg agatcatctg cacttcagta ttatggtcgg cggaaaattt gtcgacccca
    19201 aagaatggtg ggatccccac tggatcaaag acaacatcac ccggtaaagc agcgtttcaa
    19261 ccggccatcg gcttttaatg tttattgaca tcccggtgat tgacattgac atggtacttc
    19321 agcgaggtaa aaaaagcgct cattttattg gccatcacaa ctgatcgatg cttcccgccc
    19381 gtacaaccga tggcaatatt gaggagcacc ttcccttctt tttcataaag cggggtcaag
    19441 agattcatta aatctttgat tttttccaga aactgtttgc tttccctgtt gcgcaagacg
    19501 tagttttgca cgttcctgtt attaccgtca aaatttttca acttttctat aaaataaggg
    19561 ttgggtaaaa atcggacatc gaaaacaagg tcggcatcca ccggcaatcc ataacggtaa
    19621 ccgaacgact ggacattgat taccattctc ttgttttttg aaaataccga aaaagacctc
    19681 tgaacaacgt cctttaattg atgcacatta aaggacgttg tatcgatcac cttatccgcc
    19741 attttcttca gggaagataa cttttcgcgt tccagcgaaa ttccttccat aataatgccc
    19801 cgttccagga gagggtggac acgtctggtc tcgctgaagc ggtggacaag agactcgtcg
    19861 ctggactcga gaaaaagaat ctcgattttg tatccttttt tcttcaggct cgcaaaaatc
    19921 cggctgtatt tatccagaaa gcttcgttgc cttaaatcca taaccagcgc cacgcgcttg
    19981 atttccggtg aagaagccag cgtgatggca agaaatttcg gcagtaaaat aacgggaagg
    20041 ttgtcgacgc agaagaatcc gacatcctcc agcgcgcgca atgccgtact tttacccgac
    20101 ccggaaatgc ccgttataat aacaacccgc aaatttttca ttttatttcc cctggatgtg
    20161 ccgaattttt agcacacatt ttataaattt tacaacaata aaaagccgca ggcccccaac
    20221 ggattcctgc ggcaaaagaa aaaacgtttt gatgtcttgt cccgttcaga caatacaggt
    20281 gcgttcctga tcgatgataa tctgatacaa ttcatcccgg gattttgccg cgatgagttt
    20341 gttacggaag taaacgtctt tgagcatttt ggatattttg gccagaattt tcaggtgctg
    20401 gccggcagag ttttccggcg caagcaaaag aaaaaagaga tgcaccggtt tgccgtcgag
    20461 cgaatcataa gcaatgccct tttcgctgcg tccgaaagca acaacaatgt cattcagatt
    20521 ggatatcttt ccgtgaggaa tggcaacccc ctctcctatg cccgtggaac ccagattttc
    20581 acgctgcttt aaaatatcaa tgattgaaga actgttcagt tttaaaccgc cttcaatgat
    20641 aacattcacg agttccgcaa ggacttcatc cttgtttttc gcctctaaac cggcattaat
    20701 aaattccatt ttaaaaatct gattcagctg cattgttcaa ccatatcttc catgtctatt
    20761 gacttgcttg atgatcacct ctagaaaagc tccgacgctt tcatttcgtt tcgataagag
    20821 tgtaacggcc gtcatcccct ctgtagatca tactgacatt ttcagacgac gtatcgcgat
    20881 acagcacaaa acggtcattt gtttcctcga tttccatgat ggcttcatca aacgacatgg
    20941 gctttaagat aacttttcgg gtttccatga ttttacttgc cgtcggttcc tcgatttctt
    21001 cctcgttgag aggtttttcc tgagcacgtc tgatcgtctt cggtttgtgt tgtcttattt
    21061 tctctctctg tttcttgagc tgtttttcga ttttttcaac acaccggtcc agagcaacgc
    21121 gcatatcctt ggcttcttct ttcgcgttga tgttccatcc gttggagttc aaattaattt
    21181 ccgtcacatt tctgaacttt tccatagaga caactacatg agcttcggcg ggcgcatcca
    21241 gatatttttt cagtttctgc attttttctt cggcgtaatt tttttgccag ttctcccctt
    21301 ccccgttcct gaacgttacc gaaatcttca tcattgctcc tccttacata attaaattca
    21361 tcgtcctgat aaccttcatc gaccgattga ctttctttgc gccggcaatc aaaggtgcga
    21421 acctctttgc caaaccgctg catagaattg aagcctcggc tgcgccgtgt ttttcaacct
    21481 gccaatcccg atgcttcgct tcggcaccag agtttcacca taaagggacc cgtcatatat
    21541 tgggctcaaa actaaaagtc aaccaaaatc ttacaccggt tgtgtctttg tccgtcaaat
    21601 cgctttaaaa tatttctttc gcttcgatga aggaagaatc cccatcattt cccggtactt
    21661 ggccaccgtt cggcgggcga tatgaattcc ctttgccttc agtaattgaa ccatttcgca
    21721 gtcgctgtag ggtttacgcg gcggttccga gctgatcagt tgccgaattt cttcctgcac
    21781 gcttttcgat gcaatcgttc cctgtgttgt cgttctgatc gaacttccga agaaatactt
    21841 caattcgaaa attccctgcg gcgaatgcat gtatttatta ttaaccacgc ggctgatggt
    21901 ggattcatgc atctcgatat cattggcaac atcccgtaaa atcaaaggtc tgagataatc
    21961 gatgccttta tcgaaaaatt ctttttgaaa ttttacaata ctttccgcca ccttatatat
    22021 ggtgttttga cgctgttgaa tgctcttgat caaccaggtt gccgattgta ccttgtcctt
    22081 gatgtatttt ttcccgtttt cggcctcttc atgactatga ctcgaaatgc cggccattac
    22141 ttcccggtaa aaattactga tgcgcagtcg cggcaagcca tcatcgttca aaataatttt
    22201 atattcgtct cctgttttga caacatagac atcgggaatg atcggctgcg ctttgtcttc
    22261 cgaataaatg ctccccggct tcggattcat ttcgctgatc agcaataccg ccatttccac
    22321 ttccttgagc ggaactttta atttatgggc gatttgagca tagtttttca gttccagctc
    22381 tttgagaaaa tctctgatta tcacttcaat aatccggttt tttacgccca gcatcctggc
    22441 ctgtatcaac aggcattcct gaagattacg cgcggcaatt cccggaggat caaattcctg
    22501 aactttttga agcactcttt ccaccagctc ttcactgaca ttttccagtt gtgcaatttc
    22561 ctgagccgtt gcgcataaat atccattggg atccagattg ccgataatct ggctgccgac
    22621 ccgttcttcg tcttccgaaa aggacgacag tttcatctgc caggtcagat gctttgataa
    22681 cgtctgacct tctgtcagca cattatccca ggatgtttcc tccccatcct tgccgccgta
    22741 cgtcatgccc accgggccgt aatcttccag gtagctcgcc cagtcgaatt cgtccgttcc
    22801 ggctcctttt tcaacattca attcttccgt atgttcgacc gcttttatcg tatcgcgttc
    22861 gacgggcagg ccatcatttt caaattcccc gatcaatgcc tcgtccgaat tttcatcagt
    22921 ggaaatttcc tccagcaggg gattttcttc catctcctga ttgatcgctt ccacaagttc
    22981 ctgacgggac agctgcagca gcttgatggc ctgctggagc tgaggggtca tgatcagctg
    23041 ctgggaaagt tttagatgct gttttaactc aaatgccata atcggaattc agtacaagcc
    23101 tgttataaat taaatgaatc tccaaaataa aattttcggg ccagttcgct ctgcgcaatg
    23161 gtgtccggcg gtccttccac caggatctct ccattgttga cgatatacgc ccggtcacag
    23221 caggaaagcg tttcccgcac attatgatcg gagataatga tgccgatgcc cttggctttc
    23281 agtttttcaa taattttctg aatatcggca acggccagcg gatcaatgcc cgcaaacggc
    23341 tcatccagaa gaatatattt gggagaggtc accagagccc gtgtaatttc gacccgccgt
    23401 ctttctccac cggaaagaga ataggcataa ttttttgcca gtgcggtcag gtccagctcc
    23461 tttaaaagct cgcgaagccg ctcctgtctt tccgcttcac tcaaatcaag agtttccaga
    23521 atcgccagga tgttttcctc cacagtcagc ttccggaaaa tcgacggttc ttgaggcaga
    23581 taattcaggc ctttgcgtgc tcgccggtac atgggatttc ccgtaatgtt ttccccgtcg
    23641 agataaatct ctcccgcatc cggcttgacc aatcccacaa tcatgtaaaa cgtcgtcgtt
    23701 tttccggcgc cattgggccc cagcagaccg accacttctc ccggtgcaat caataagtcg
    23761 atctgattga ccacttttct cttgttataa attttcgtta aacctttagc cgataaatcg
    23821 ctcattttaa atcatcagtt tttcatggtt gactggtttt ctgtctgtct gcgctcctgc
    23881 ggatagataa ccgccttgac gcgcatgcgc tctccgcctt ccacaatacc tttatcttca
    23941 tcaagcagaa taatcacccg ctcgcctctg atcgaacttt ttccttcctt cagttccgca
    24001 ttccccgtca tgataatttt attttccgcc tgaacatata cggcctcatc ggccgttgcc
    24061 ttccgcgttc cctgcaccac ggaaacattt ccccgggcct cgattttttc cagatccccg
    24121 gcttgattgt cgcgcgcctt ttgcgccttc tctgttttcg gctgttgccg ataatgcaaa
    24181 ataagttttt ccgcctttaa tacactgtcg ccctgaacga ccgtggcatt tcccgaaaaa
    24241 acgatctggc tccgattctt aaacgcctcc attcttgcgg ctgtaatctg aatcggcttg
    24301 tccgtgcggg cggtcaatgt atttttaccc gggcagattt caacgtagca aaagaaaaaa
    24361 aacagcatta aaaaaatgta tgtgtgttta cggatcattc ttttctcaat gaatgtttgc
    24421 cttgaccatc gacggaatac tcaattcccc gctgttcaaa aaaatggcca atccctgccc
    24481 ggttattctg atgcgtctgc tttccattct gaccggcgcg tccgtgaaaa attttctttc
    24541 ctcatcgctg tattggacat actccgtgaa aaaattatcg ccgtcatccg acgtgatcac
    24601 gacattgccg ctgatgttca tgttcttctt atccgtaaaa atccggccct tatccgcctt
    24661 catttcatac tttttaccat ccgccgttat caccgttacc cggacctgct ccaaaacagc
    24721 aagattttgt tttttatcgt atgacgccga tgccgctttt acctcccatt ttcccttatt
    24781 ctctccaact tccgtataga caaacccttt aatttgcaga tcaacgttat cctgcagcgc
    24841 cttcaataac gttttcggct tattgatgct atccctgaag attaaaaaag ctgccagaaa
    24901 aaccgccaga aaaaccgtta caacggagaa gaaaactatt gttttttttc tgttttttat
    24961 cacaatccga aacatcccgc agaaactttt tttatttgtt tttattatat cactcagaat
    25021 gcagggtgta aagctataag gggtgtactg cgttataaaa accgtttaaa aaaagccatg
    25081 acgaaatgtt atttttaaaa ttcatatcgc gcggcaatct ccccccactt tccctgagcc
    25141 tgcagaatca tctcacagac ttcccttacg gcgccctttc cacctttctg cccggttata
    25201 taatcaactt gctttttgac caggtcggat gcgtcggcaa cggtcgctga aaaaccgacc
    25261 ctgttcaaca ccgggatatc cacaatgtcg tcccccataa aagccgtttc gtctggcgag
    25321 agcttgtatt tttttagaat tttctcaaag acttctttct tgttgtatac tttctgatag
    25381 acatctgtaa tgtccagatc tcttgcccga tgcgagacaa ctttggattg acgaccggta
    25441 ataatagcca ctttgatatt aaagcgctgc aggattttca atccatggcc atcgcggaca
    25501 ttgaaatgtt tcgtttcaca cccttcatca ttcataataa tccgcccatc cgtcatgatg
    25561 ccatccacat ccagcatcag cagctttatt tttttgagtt tttccttgag attctttttc
    25621 attttttatt cagccttttt tcttttattg atttttcccg tttgacggcg ttttgactaa
    25681 agcgtcgatt ttcatcagag tgacaagaag gtcttccagc gaatccagat agagtgaatt
    25741 tgcaccgtcg cacagcgctt tctcgggatc gggatagact tcaaaaaaga caccatcaac
    25801 accggcagcc acggcagcgc gcgcaagata cgtcaccatg tctctctgtc ctcccgaaga
    25861 cgccccccgg cctccgggca actgcaccga atgcgtagca tcaaaaatga ccggataccc
    25921 catcttacgc atgatcggca gggaacgcac atcaaaaaca agattattat aaccgaacgt
    25981 tgcgcccctt tccgtaatca tgatgttgtc attgcctgcc gcacgagcct tttcgatgat
    26041 attggccaca tcccacggtg ccagaaactg tccttttttg acattgatca cacgcgcctt
    26101 ttttgccatt tcggtgacaa aatccgtttg acggcataaa aatgccggca cctgcatgac
    26161 gtccagaact ttcgaggccg gatcgatttc ttcgaaacga tgaacgtcgg acagtaccgg
    26221 cacacccagt ttttccctga tgcgctgcaa tatcgccaga ccttttttca atccggggcc
    26281 ccggaaggat ttaatcgacg tgcggttggc cttgtcgtaa gacgctttga agataaaagg
    26341 cattttcagc ctctccgtga gttttttcaa ataaagcgca atcgccatcg cctttttctc
    26401 atcttccaga acgcagggtc cggcaatcaa tataaatttt ttattatctc cgattgtgac
    26461 attacccatt tttatttttt tcatttttat tcactccctg tattttgccg ctttctctcc
    26521 tgtaataatt gaatattcaa tcgggggatc cgttgagcgg cttttggcga ttccggattg
    26581 agtgcatgag cttttttata ggtttccagg gctttctgat atagcccttt tttctcatac
    26641 gcttctccga gattatcata aatatcggca tcgtcgctat cgaatttcaa ggccgtatta
    26701 taatgtttga tggcctgatc gtaattaccg gcagccgcat aggcgaaccc gagattggcg
    26761 taagatcccg cctttttagg ttcctgtttg gccagcttct gatagattgc gatcgccttc
    26821 tgataatttt tttccttcag gtaaagatct gccagcgcgg tcgacgcgtc ttttgacgat
    26881 gcaggcatct tttcgtacac ggcaatcgcc ttctttttca tgcccatctt ttcgtaggta
    26941 tccgcgagat tggcacgcaa aaccggttcc ttcgcgccca gttgaatggc cttttcgtaa
    27001 ttcgaagccg cagcctggta tttttttaat tctccatagg caaaaccgag attcgcataa
    27061 accgaggcct tgcgcgatga ttttgttttc agcctctcat aatagttggc cgcctggctg
    27121 tactgtttgt tttttagtga cagatccgcc agcctctcca gggcatcggt atcgctggga
    27181 ttgatcttca gaacatggcg atattctctc gccgcatcgc catacatttt ttttctttcg
    27241 taaacggcac cgagattgaa ccggatggta ggctcgttgg gattaagcgc ggccgccttt
    27301 ttcagacttt gcagttcctc ctgcgccttg ccgcttccgg catatgccac cgccagattc
    27361 gcatgggcta ccggattttt aggctgtcgt ctcaaaattt cccgataaca tttaatcgcc
    27421 tcatcggttt ttttgagttt cagataaata tcaccgagcg caaacagcgc ctcctccggc
    27481 tcccgggaat gtttgacgat gtattggtat tcgtcaatgg cgttgctcat catattgccg
    27541 tttttgtatg cgtccgccag tctgaatctc gccgcgtcgt tatgcggttc cagccggacg
    27601 gcttcccggt aatgttgcgc cgctttggac catgcttttt tttcctcgag agcaagtccc
    27661 agcgccagat ggccctccgt ttctttggga ttgattttaa tgaggtcgcc ggcgagcttc
    27721 gctgcagcgc cgaattcctt ttgtttgata tgaattttca ccagctccag catggcggtt
    27781 gtgtcatcgg attggatcgt caggacctgc tgatacagtt ttttcgcgtc atccgttctt
    27841 ccgtgatgcg cataaatacc ggccagaatc ttgcgcacac ggacatcgtt ttgattgagt
    27901 tcgatggctt tctttaaata gtcaatctgc tgttcgacgt tggaagattc tttcgcaaac
    27961 ctaaaccagt cttgaggtgt aatgacaatt ttcagcggca cggacgcgat cgtgtcattc
    28021 agataatgca caaggatttg ataattgttc accgtaccgg aacgaccgct cattcctccc
    28081 gtcctgatca gcttattgac aaaatcaata cccttcatca aaacaccgag gtcgctgctt
    28141 ctgctgctga gcccttcaaa ccggacgtag gtatacttgc cggataaatc atcactggcc
    28201 accgctttga cgacaaactc atcgcggtat gtaatttcca gcacgtcatt ccggtatatg
    28261 cgcatgtcct gaccgttttt ttccacgtcc aaataataga attgcggctt ctccttcaga
    28321 acataatacg aaaaggtatt tttgatcctg gacatctgaa aagccagcga caaaaaaaat
    28381 ccctgataaa aaagaagcgc cacaacggcg atacaaacaa caacggcggc tgcaatggat
    28441 agcgttttta ccggcaacct tccatttaat tttggtcttc tatcctgtct atcctgatgg
    28501 atcatcgcat ttctcgtttc tgcatgacga tgcttttgat aacggcgttc ctgttgttct
    28561 tcaagcccgc ggatgaaatt tgcggtgaac ttccttcaac cgttccctcg taatgtgggt
    28621 atagatttgc gtggttgcga tatccgaatg tcccagcata acctgcacgg tacgcatatc
    28681 ggcgccgcct tccagaagat gcgacgcaaa ggaatgccgg aacgtatgcg gataaacctt
    28741 cttctgcaat cccgcctgac gcgcacaggc aaccacgatc ttccaaaacc cctgcctggt
    28801 gaattttttt ccgaaccggc tcaaaaacag aacatccgtg ctttttttct gcatgaattt
    28861 cggccggatt tcatccacat aacgcctgac acaatcataa gctattctgc cgatcgggac
    28921 aattctttct ttactgcctt tccccatgac aatcagaaat cccacctgcc agttgatgct
    28981 gttcatcgtc aaattaatga gttccgacac acggatgccc gtcgcgtata aaagttccag
    29041 catggcggta ttgcgaatgg cagcattatt ccggtttccg ggctgagcca gaatggtttt
    29101 catttcttcc ttgctgatcg tttccggaag cttcatccag actttcgcca gctcgatatg
    29161 ggccagcgga ttttgggcaa taaccttctc cctcagcaga tatttgtaaa atcccctcaa
    29221 agcggccagc gctctgttca tggaattcgc cgccaaacct tcttctctaa gctttgtcag
    29281 ataatccacg acgatttccg gcgttacgtt gtctatatcg cttattttcc ggttggtctg
    29341 cacaaaggcc agataccggt gcagatcccg gctgtaggat tccagcgtgt tctgagccat
    29401 tccgcgttct atgaacagat aatccagata tttcattaat aatgcccgca tatgactttt
    29461 taacttaatt atctttctga agcaaagatt ttttattgac ttatttctga atatcataaa
    29521 aataggaaca gaaacctctc taaaacgtta ctgacaagga accgctcatg aaaaaaattg
    29581 ttatcgcttc cgatcacggc gccgtagctt taaaatccga aatcatcgct tttttgaaaa
    29641 caaaacagtg cgagttgaag gatctcggag tcaacagcga tgatccggtc gattatcccg
    29701 atatcgccgt gctggcctgt acggaattca aacgaggcgg atatgatttc ggcattcttt
    29761 tgtgcgggac gggaatcggc gtatccatga ccgcgaataa aatcaaagga atccgttgcg
    29821 ctctgattca cgacctcttt accgcagaaa tggccaaagc ccataacgac gccaacttta
    29881 tcgctttcgg cggccgggta aaatataatg tgcccgtcac gaaaatgatt gaaaaattca
    29941 tggaaaccga atttgccggg gaaaggcact gccggagagt cggtaaaatc atggacgctg
    30001 aaaaataata ttcagacagc tttttcattc aggaatccaa atgtcggata ttcttgataa
    30061 aaaaaagatt cgcaaggtgt ttcgcaacgt tcagaaacac ctgagcattg cccagcttat
    30121 ccgccgtttt tccaccaaca aagaagatat ccgaaaagca gccctgaacg gggtcaacct
    30181 gtcccgatgc aggagtgttt tggaactggg ctgcgcgttc ggtgctttta ccgaggcgct
    30241 gagaaacaaa ctgcatgacg gcgcaaccat tacaggcgtt gatatcatct ccgaatacaa
    30301 gccttttttt ctcgatgcct gccgacgggc cggttacgaa ggaacctttc tatcttccgg
    30361 aatcgaaaaa ataaaaaaaa tcccatccgg attctttgat ctggtgatct gcagttacgc
    30421 tttatacttt tttcctgaga tcattccgga aattgcccga atcatgaaac caagcggttt
    30481 ttttattacg attacgcatt ccagcaacga catgcgggaa atcgttgaca tcgtcaaaag
    30541 aattctcggg cgacatcatg cttccggaac caaccggtct ctgccgatag aaattatttt
    30601 cgatcagttt tcggctgaaa acggcgagtc cttgcttcag cctttcttca agacgataga
    30661 ggccatcgac tttaaaaata cgctggtatt tcagtctcag gaaatcagat atttcatgga
    30721 ctattttcaa ttcaagaaat cttttttcct ctcggagcag gacgccaaca atcaaacgat
    30781 catccgtcag ctcttcaagg aattgcagga aacactatcg aagaatcaaa ttgtcagtat
    30841 gtgcaaagac gacagaattt tcatctgttc gcaaccttca accggcaagg actccccatg
    30901 aaaaaacagc gcaagcattg cctttattgc ggcggcaaaa ccgtcaaaaa atatgaagat
    30961 aacgtccggc gcgatttctg tcccgtttgt aattcttatt tttatgaaaa tccgctgccg
    31021 gttgtttcct caattctgga agattcccgt cagattcttc tggttaaaag aggcaagcgc
    31081 ccgtcaaaag gattgtggtg cctgccgacg ggatttgccg aagccggaga aagtatcgag
    31141 gaagcggccc tgcgcgaatt ggaagaagaa agcggcataa aaggaaaaat tataaaatta
    31201 ctcgacgtcg attcctataa aagtcgcttt tacggtgatt tgctgtttct gacatttgtc
    31261 gttcaacaaa cgggcggaaa gctttgcgcc ggcgatgatt gctcacaggc ccgattctgg
    31321 cctgtccata aattacctcc cttagccttc cgctccaaca aacgcgctct ggatgcctac
    31381 attaaaagca gaaaagacta ctgggctatt tttgattcca tagagcacgc ggacaaaaaa
    31441 tccctgccac ccgccgtggc aaaaaattct ctgacgcgac gcctggtgaa tgtcatcatc
    31501 aaaaacaaag aaaaaattat tgaacaatgg ctggaagacg tcatagtcaa tccttccact
    31561 accgagtatc acaaggtgga acggacggtt ctgtataaca tctgcgacaa aattctctct
    31621 cagttaaccc tctggctcgg agggttgcac gatgttgaca aaatcaggaa tttctatacc
    31681 aaactgggta aagaacgaag gatttcaggc tttaaaatca gcggcgcctt gagcgcactc
    31741 agtctcattc gcaaacacat ctggaccttc gccttggcgc aggacgccct gcagaaaaat
    31801 ctggagctgc tgatgacctt cgaacttcaa cgaaggatga cggttttttt cgacctggca
    31861 actttttatt taactaaggg acacgaagag aaatagtaag atcttttttt gagaagataa
    31921 cctattagta atgtcatacc ggtgcaggcc ggtatccagc taacgtaagc gagtcaataa
    31981 tttcccagat tccggtttac accggaatga cggataatgg atattaatta aatgtcaacg
    32041 tcaagattgg aggtgttact atggaattca aagatgttat aaaacaaaga cgggctgtaa
    32101 atttttttga ccccaccaaa aacgtaaccg atgcgcagct taaacagatc atagaaaccg
    32161 cggcgttggc gccttccgga ttcaatctcc aaccatggag ggttatcgtt ctaagagata
    32221 aagaaaaaaa agaaatgttg agaaaagtag ccatgaatca gccgaaaatt accgaagccc
    32281 ccgtcgtcct gattgttctg gcggatcgtg aaggatggaa gaaaggcaat cccggttttg
    32341 aaaaagattt tgtcgaatcg gtcaaagagg gcatca
//
LOCUS       DAOSME010000026        30158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_1973_length_30158_cov_21.226389, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000026 DAOSME010000000
VERSION     DAOSME010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 30158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30158
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1973_length_30158_cov_21.226389"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..2546
                     /locus_tag="PLG98_05625"
     CDS             273..2546
                     /locus_tag="PLG98_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32768.1"
                     /translation="MSILSQIIQRDTKNAKVAKEGAYDKLKNTHGQKFIDDITGFYTA
                     ANKQRWLHYNAEQKSLIAICENPFHAMAEINTFHENGRTNHTIWYANEHTSITQVIDD
                     DIIIVPWTHPGFQIAITEDLNQKHDVNDPRYRLSGIIPLARARFQKVFPVIIGIYDPG
                     GHVGQIEKVATTTGLKAVKLNMTKDQVDAFISKMRGVLFVTGAPGSGKTTVAFQRMRF
                     LFDEGETRIGVEHRPENSKVFLANENLIKHSKDLLEKQLDIPPSIVSLIKNFIEEYLD
                     DAWRFKHEALFLSRDIPNALERRAREAFFSTCSVDDMKKCWKTYELQIAEKISTASHS
                     EWAKLQYRRLEQRESQSNLITRLDEFAKRQQKRPFSSTPTSSEVSMDRLYWHCSRQYD
                     DLRKELTRPEVEEFDGLFSKWLYFVYDPIECLRKYFGSKTHEGEIRIRQGTGARLNEE
                     LIVRDILEDWEKRRYRREEIGWIAWLLRFVLPTTEDAKDRFREMPNAYSPVSGKHGPW
                     QHIVIDEAQDLSVVEASLLSSFILRDGALTISADFRQVVSPVHGMKNLDAFKIGCNLI
                     SKADDFTQFPFTKNLRQSGQIGNFLRGFYEKVFGELPQFMANDEMKGPKPQLHLMPYV
                     NFAQTIRKMLNVFKEKKVTGSMAFLQINEDEDEMIRYREMLEKENISLADIWVSDSAD
                     GAMVTTSIERIKGLEYDICFVVGLEKAENTMLNFNKNRAYVALSRPAQRLYMFCEHIP
                     GLLHGMNNDLYDIFDAR"
     gene            2558..3412
                     /locus_tag="PLG98_05630"
     CDS             2558..3412
                     /locus_tag="PLG98_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32769.1"
                     /translation="MFPEFEKFAKEENLLEIKLAGNLAAQIPRILGQGKTKAIGLDLP
                     TSPIERKQEFVTLDLSPSFNMPAAVREEQLRVKAELDREMKQLCSAEGARSLASNVIR
                     NLNIDPPGNMKNNYFYAGRFLLMPDAQRTSWSWSITPYYPIIEKIPPDFDYLVKAFPV
                     AEEKLNQIVKPVEVFEKQMELSWWLARHFAEASDKVLLSDVARMYVIASQDKRFWTKP
                     QRRNFTDVPDASFIANLINYKSNSPEPVFEFMPATLNDSYRKNVYQIPLDREGTQTRP
                     YVYIVKKR"
     gene            3434..4729
                     /locus_tag="PLG98_05635"
     CDS             3434..4729
                     /locus_tag="PLG98_05635"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOE32770.1"
                     /translation="MAVLDKSEAIRIKAQLISNPVAPGVYSHFINVGTNNIIEPLEDN
                     YFRTEMPLGISCFKYLQGYYGSGKTQFINSLAARAWQNNVVTAIVNVGIDCPFNSPLA
                     IYQSVVSNMIPPPAPGTDPEDERGIEIIFRNYINQKLKQYGIEGGKAVPAEVRQLIVQ
                     PFTEMYIGAPDMQAAIAIQQLGKLMLNVACGGTATPADMEIISWMRGDNITSRHLRDM
                     GLHKPVKDDSAFNRLKTIIMFMTKRMGMKGILIAFDEGTRTVSFRRGSVKQKQAIENL
                     LTMINDGADGKFPGVMFLYAATPDFRNEVIQKYPALNDRIGNIAFKPGSPMVPFIDLD
                     SLDTRSIVREIGARLLEVFSIASGVEWKQQIQKNNIERLMMAEEKSYGMGSEIRRSFV
                     YHFCIFLGHQIKEQFELSDKDAEAFILDHQLPRTEDEEV"
     gene            4729..6198
                     /locus_tag="PLG98_05640"
     CDS             4729..6198
                     /locus_tag="PLG98_05640"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOE32771.1"
                     /translation="MQLTKGDKVTHPDYGPGIVNQVLGRTVGVNFAGEDMDVDISTLT
                     PIGKIAPPVPDNGNEDRSQAKTEFRAAFEAINLGVAPPDTGQLIDLTIRSDELKKRVS
                     GWLTNAPKSGLCKVVFGYYGTGKSHMLKFVRCMALKAGWAVAYLEFDPKAADPAKPHL
                     VYQNLMTALEFPKREDGRQTEDFIGFVKEVRDHWAQKNIRNNDIFRSNLWFSRAFEVL
                     MKYPHMPDVMEDYRDACLWLAGNHNSFQTVNTLARDKGLNIRVPRMPITKETADIYVH
                     HLVVVNALCKLLGYKGLAIIFDEAEHVRGFNVRRRERANNFFELLARSAHLPNPDDDD
                     PTMNDHGISVPPYWNYGPHFALFVGLTEGETFLDLSLNLRDACLFLRSEEDRIMLANP
                     SREDYVKWCHLFLKKFCSFYALETTQLKTDDSKEKIVDCLAQNYQAAQNGIPLRNFIK
                     LASLVPCMLLSHPGMTFEELMAHLKTTMAEFLGNAMPWE"
     gene            6179..8827
                     /locus_tag="PLG98_05645"
     CDS             6179..8827
                     /locus_tag="PLG98_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE32772.1"
                     /translation="MQCLGNSESNLMMRWMPDAWPVFFHKRGPRPIQQNAIPLILKGE
                     SVFLTAPTASGKTEAVMAPLYQRHISFKHDHLSVLYVAPTKALVNDIYYRLSDYLGCG
                     KDFSGICRYTGDHHDFKEPMGAFALIATPEALDSLQLRHPQKLEHIRAMVVDEVHFIH
                     GKARGEQLRYVIDRVRSKSRSPLNPKDVFQVVAMSATLNDMESVGRLWVGNQVKYVTA
                     EDPREIEMHHLPVPMGKIQQRATEIAAAIRNYIEIQQMEKVLIFANTRNDAHHLGFVL
                     HDVFKGTRWPVYLHFGILETKVRDEIESDLKKKRYGICVCTSTLELGIDIGDIEAIML
                     LSPPPSVSSFLQRIGRGSRRSEICKVVTLVRNDNERVIYEALLDLARHGKLEPIHEYS
                     RPSVAFQQILSHAWNGLRKEKPLTLKNLAARTGGRDFGEIVNSMLEEGHLKLNHGALI
                     PSDRLIELGDRSVIHTVIAGDGAKPVYDGVTGDLIASIGEGAGDGLYFLGGTLRRIAS
                     GEKNSYVLEQVEPKGPRQIGKIPAARGGRGISRTLVWKIAEMTGHDPRIWSWTNDRLV
                     TWGGWVNNLLLKFILEQHGYGTISSFDAFALEGIEINKKDEINPDAISAFVRNAEIHL
                     SLREAEKFREPSRFYQYLSTNLKTKESESAIPVPEFTQWLNECLMETEQTNEIEQEKT
                     ALAEECAASPHYENDDMGSLTVLKESVRIQTFSGIAFDLLNPKPEMILVEDIIHSLAL
                     VNRFNGAAKFPYSVAQHSLYVASLLPPELKLQGLLHDAAEAYVGDMVSPLKKIMTEYK
                     EVETGIARVVAEVFGLARPAPPAVKKADLAVLSAEREQVLLPSYGPWYKNFPSPADIR
                     IEPMSWVQVKEIFSSELHSLLESPLE"
     gene            9026..10849
                     /locus_tag="PLG98_05650"
     CDS             9026..10849
                     /locus_tag="PLG98_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE32773.1"
                     /translation="MAVMIPDDVQDFCTEGERHFYGFLKTAAKPDSKYLAWYLPDING
                     REPDFILFCKDTGLIIFEVKDWALHQIQAANPQNFTLTINGRNEERESPLKQARTYCH
                     ILMDKIRDDRRLLSKDPQHATNPKIPIHEGVVFPNINKYEYIQKAFDKVIHSEQIFFW
                     DDLHPESDICRDLSGQCFSTVLQKKYAAPFHFNLTPDEMQHLRQLLFPMIRIELPDRT
                     SKAQRQREKDRIRLLDHHQESIARKFDGGHRIITGPSGSGKTLVLVHRAALLQQYNPA
                     VKRILFVCYNITLVNFIKRLLSEKQVPLGENGIEVVHFFELCAKILGESVTWEKEEPA
                     YYDLVIEETLKKVKDYPEKYDAVLVDEGQDFSDDMYSIVVSLLNPATNHLAIALDDNQ
                     NIYRRKQTWKDLGIQVRGRVHRLSWVYRNTSAIVDFARKFLNEKEEKAEHPPHQMELF
                     QNFLSPAGTEPEIRQYPDVDELINHVAKQINHLHNDDGYPLSEIAIIYTQKSPDSLPG
                     VHFPHLMIEALEKYGIYCNWIAEDYRAKRSYDVTTQSVTISTIHSVKGFDYACVFVVG
                     LDWLQGDRWTEEQVKNLTYVAITRARERLFVPHLHDLILPK"
     gene            11039..16324
                     /locus_tag="PLG98_05655"
     CDS             11039..16324
                     /locus_tag="PLG98_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE32774.1"
                     /translation="MDVFALRNRLVADYRSYTRSFIKIADQRISELVDTNLTAGAFWP
                     EPLLQLNPIFKSGGTIDELVRKNVLNPECAKIFRIDKTDTDFTGKQLNLHTHQREAIL
                     KAKEGKSYVLTSGTGSGKSLTYIVPIVDHVLKNGTGRGIQAIVVYPMNALANSQDEEL
                     GKFLKKGYPEGRSPVTFARYTGQEKGEEREAIRQNPPDILLTNYVMLELLLTRTEDRE
                     LVRAAQGLKFLVFDELHTYRGRQGADVALLIRRCRLAFGGQNIICVGTSATMASEGTV
                     EDQKKAVASVAQTLFGVPFETSQVIGETLSRATNELDHNDPQIVQLLRKTIEADEAPP
                     ADYESFIKHPLASWIESTFGIKTAEGSDRLLRQLPRRLEGNDSTAEELAGLTSTDEQR
                     CAEILRRYLLQGSYLKASTSSRFPVFAFRLHQFFTRGDTVWATIEPEADRHLEMSKKV
                     SKPGEPEKPLYPLVFCRQCGTAYYRVKIISNNGDKYLLPREETRQADDDESTFGYLYL
                     SETSPWPRGSGPELLGRVPDALKETNPRGEERIRPDAKNDIPETVYIDATGKVCAEGQ
                     GVEAALIHRNFLFCLEPSCGVAYTRSQKAERGKLATLGIDNRSTATTILAVRSLIELQ
                     GDRDLEAKARKLLSFTDNRQDASLQAGHFNDFAQVALLRSALYKAAQARGEEGLKHHD
                     LSRSVFDAMQLSFDEYAVDLEVRGPARNATNDALRRVIEYLVYRDLQRGWRVTAPNLE
                     DCGLVRFAYEGLNGEESLLSEQRLWDEGFSIRTDNGEEFLSIPEALRLCTPEKREEII
                     RTLLDVLRRSLAIKVDVLDPQKQFDLVEQTKPRLREDTVWYLEDGRDLIKAYVAYPRA
                     RIRNNRYEEYNFLPVSSYSGYGRYLKRTLKNCVPPGRPFGRQEVDEVIRFLFLALKRY
                     GIVEQVRSRPDGSDPGYQINPAAIIWLPADGEIRPIDRTRLLDAGELPPEVNRYFVEC
                     YRHFVDLKCLLEAREHTAQVKAEDREKREIRFRKGAEPGGLPLLFCSPTMELGVDIAQ
                     LNLVNLRNVPPTPANYAQRSGRAGRGGQPALVYTYCAGRSPHDQYFYREPNQMVSGSV
                     VPPRIDIRNRDLVKSHIHAVWMEAAKPDLGKTLTQVIDLTPADGRLPLPIKDEILREL
                     RSPVHRATAQTRANQLIESIQDELNNAPWFHDNWTKEILDQIERTFDVACERWRSLYR
                     AAVRQREIHHRIIGDHARPEMERNQSRRLRAQAESQIKLLTEAEGIYEGDFYSYRYFA
                     TEGLLPGYNFPRLPISAYVPGHRQRNSRDEFVSRARFLAISEFGPRALIYHEGARYRV
                     YKVNLDFGTDDIEETHNLVTATMKRCPVCGYAHIEQDRNLNEICDNCGGALDGSSQID
                     NLIHLQNVSLKLAQRITCDEEERQRFGYRIVSAYRFPEIGGRLDRRDAEVYCEGTLTL
                     RLKYGDSTELFRVNLGWANQIIGQPIGFNLDLERGYWWTNRADAEDLQDANTQGRSQR
                     VVPYVRDTKNALVMSFVPVRPLPETASLQAAFKEAIQKYFQLEPRELECVPMPSPQGR
                     RELLFFESSEGGAGVLRQVAEDPSVLPKLARIALEICHFDPDTLEDKGALTCGKACYS
                     CLLDYGNQPDHKDLNRYLIRDLLAQFSKSECKPSGGTGTRAERMNALRKRCDSQLEKR
                     WLDMVDRLMLNPPSDAQYLIESCSTKPDFYYRDFNTAIYIDGPPHDEPEQIRKDEEIT
                     RKLIANGYIVIRFHHRVDWNAIFNQHPDIFGRPQT"
     gene            16321..19158
                     /locus_tag="PLG98_05660"
     CDS             16321..19158
                     /locus_tag="PLG98_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE32775.1"
                     /translation="MSTPNLVRPGTLINARGREWVVLPESSEELLLVRPVGGLDEEFA
                     GILPAIEPVESSSFPLPTLEDLGDFTSGRMLRDAARLSTRAAAGPFRSFGRIAAEPRP
                     YQLVPLMMALRLDPVRLLIADDVGIGKTIEAALIARELLDRGEINSFTVLCPPHLAEQ
                     WQRELSQKFHIDAELVLAETIHRLENDLPVGVSIFDRHRFTVVSTDFIKSSRRADDFI
                     IKCPKLVIVDEAHGCTLAEGVGRGRQQRFDLLKRITEDDDRHLILVTATPHSGKEEAF
                     RSLLSLLRDEFANLPTDLDREERADIRRKLARHLVQRRRADLRHYLDADTSFPERYDK
                     NPEPTYTFSKEYKQLFDDILSFAREYVSEDGTSRRNQRVRYWSALALLRCVSSSPAAA
                     AATLRSRAAVDEAADIEIDEVGRRTVLDQDDTDDVAVIDFSPGSDDETSATNTRRKLL
                     EFARRAESIPTEADRKLQGAIKEIKELLKEKFHPIVFCRFVDTADYVANHLREALGEK
                     IRVQSVTGLLPPAEREARIESLVSESEKYVLVCTDCLAEGVNLQNDFNAVLHYDLAWN
                     PTRHEQREGRVDRFGQKSLQVRVTTYYGTDNPIDGVILDVLIRKHKRIKSDLGVTVAV
                     PGSSEQIAEALFEGALFREKTHAHPDQLRLDFIDEMETKKKDLHVQWDNARDREKASR
                     SRFAQHAINPEEVAQELASVRAAIGRSEDVSRFFSDVLQLTGVPFDQKANAVRVHVSS
                     ETPRALRQAIGFDDPFAGRFELPLGEKEIYLARTSPVIEGLANWVLDQALDPVARDGR
                     PIAARLGVISTSAVHERTTLFVIRFRYHLEKAGTDEAILCEEVVPLACTGPQDNPTWL
                     PQDESEKLLSARPERNLIATAINQQTGLLQELLPAFRQSLEIVAKERADIQLAAHERV
                     RAALKTKTRMAIKPVLPADILGAYVVLPRLI"
     gene            19161..23186
                     /locus_tag="PLG98_05665"
     CDS             19161..23186
                     /locus_tag="PLG98_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE32776.1"
                     /translation="MAKRDQTFQSIRSEGGLLPPDLLRRILDPKGKIPGTLPEDYGLP
                     KGERLNEVITQSWNRLRRHWAEFTEAIEKLSSSDTGTGITNEKWSLPLLRELDYGTLP
                     ASAGPEINGRTYAINRFAGPAPVHLIGCKLSLDRRAAGVRGAAAANPHGLVQEYLNRK
                     EESLWGIVCNGYKLRILRDNLALSRQSFLEFDLETMFAGELYSDFVLLWLVAHATRFI
                     PKVEGRVETCILEEWTKLAEEQGTRALGELRTGVEKALQILGEGFTSHPKNTDLRDAL
                     RTGKLSPAGLHGQLLRIVYRLIFLFVAEDRILEGMPILHPRDDAEAAGRARERYAAHY
                     STSRLRDLAGRIKGSRHGDLWRQFQIIVHALSGEKAYSATREKLALPALGSFLWNPAS
                     TADLNGAELINYDFLECLRHLAFTRQDKILRPVDYKNLGAEELGGVYESLLALSPQIS
                     ADGARFTFAEFAGSERKMSGSYYTPDSLVQCLLDSALDPVVEEAIKNKKGKDAEDSIL
                     SLKVCDPAVGSGHFLVGAAHRLARHLARVRALAEGESEPSPLLHQQALRDVIGHCLYG
                     VDINPMAAELCRVSLWLEALEPGKPLTFLDHHIRIGNSLLGTTPELIAKGLPEETFNP
                     IEGDDKKICTALKKRNKQEREGRQTDMMHAMVAESAEQYNTLEARSRSIDDAEDESIE
                     DILYKTEQFHKLMASPEYRRARLLADAWCSAFVWHKQKGVPEPITTDTIRRLNDNSES
                     LSQSQVKEIMRLSRQYRFFHWHLAFPEVFANGGFDCVLGNPPWEKVTTLEREFFSTIA
                     EIAQEKRSDKRKAKILLLRDTFPERFNQWIEQLRYDAAQMQFLRNSKYFPLSAVGELN
                     LYPLFVELAEHLIRETGTVGMIIKTGMMLSPTWADFSSYLIDNDRIDSAFDFRNWNGW
                     FPDIGYHERFSLLTMKKGRRKRDIVLGYYLDDTMEIKDSPKKFTISRDEAIRLNPITK
                     TVPAFESRQHKTCVMAVYDRFPVLGDKASEWGVHYTTGLHMAAEADKLRDFEELSAEG
                     FAPVEFQWMKKEVVTFVPLYEGKFIHQFDHRFASFEGILREKRFGIKAGTHTPSTDQK
                     SNPAYHIMPRYWISEDDAASDREARDLRSSWAIAFRDTTNVISNFRTSVACICSGVAF
                     NYKAPNIVLMGPSSQERARASLLFVTLMNSFSFDYVVRQKFFGANFIKSILLQLAAPS
                     MTEIQRHQQFILPRALELSYTSWQLEGFSTECGHKGPPFIWDDMRRQQIRAELDALFF
                     HVYGFSVDEAAFILDSYRILRDKDEEAFGEYRTKRLILEIYDEMAEAIRTGKPYQTRL
                     DPPPADPRCCHPPRERKETEE"
     gene            23183..24883
                     /locus_tag="PLG98_05670"
     CDS             23183..24883
                     /locus_tag="PLG98_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOE32777.1"
                     /translation="MKNKTKQDKIITKRRRISVVLNDMLAGKYAIPRLQREFVWDGQR
                     AAKLMDSIYKEMPIGTSLIWDTTKSKKLYLRQQYNVLPHFNDKNKRTWFLIDGQQRMS
                     VLHGIHQGKPIKNSKGKEINFRNIVFSLEKKKKSPDGKEIEQRILYRKPQQGRFVSLS
                     DILSPQFNHHFNGLKKSSLSALNECRKRIMNYKIVFISTRMSDIDEVKECFLRINTLG
                     MKVSSADAIFTRAQTLDLRDFVHEVRNCIDNFKDIDEMPILWAMTAIRGECYPGKNRI
                     DAAVNKIEEKAQKKNHVKKELNKEWSLLKECFRKSAVYLENEFRVLSANYLYSPYMLS
                     MLALFHYFNNKKSPNQKQKKQIRRWFWVTTTARNYSGQLFYKSIQYDSGFFRRLAKND
                     NARFPLSTDADFRDILSTPYDKTKSGMGASFYSLLFKKKPVSLLDDALNEIQLERYLT
                     PVNKDRHHIFPRSLLKKIGIRQTEYNSICNICLLTAEENQSIGNKHPSKYLKEAKRNK
                     NLFGKKMSHHLIPSFDNNQGLWAKNTKKGFHKFIKERQILIKESLEKEAGIRLFRVDK
                     "
     gene            24964..26691
                     /locus_tag="PLG98_05675"
     CDS             24964..26691
                     /locus_tag="PLG98_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOE32778.1"
                     /translation="MEYIPIRVSDIIRQVNHDIFLPAIQREFVWKTDRIERLFDSIMA
                     DFPIGSFLYWRLEQKNKDMWPIYEFIRDFDDDAPHNAPANMAGINKDITLVLDGQQRI
                     TSLFMGLKGSYKFFYYRTHATKLYLNLLKNPLPNEDNPEELTYGFAFRENAEPDDNDN
                     PQLWYLVGKILNYEDAEDAKADMKTELAGLTEEQRENANKLIGRLHNRIHTTLVGNYY
                     QENSQDYDKVLQIFVRANSAGQPLEYSDLLLATATAKWENLDARSEIHDFTDSINEIG
                     TGYNFGKDFVLKACLYLSEIKKDDEILGLPIQYKVKNFTKSNLRIIENNWEDIKISLL
                     TTVRLISRFGFNSKNVVAPLALLPIAFYLKKRGNSSFDTSSSVNDADAQVAIRKWFIF
                     ATLKNAFGGSSDTTLTKLRDLLINYDSKMTFPADVLYKALGIEPHLQDSEIEKILGYG
                     YQGRYTNLILSLLYPDRDWKDAVFHEDHIYPKSEFQVRLLKKRGYDEPRIQSYIAKYN
                     TIANLQLLTESENLSKNATLFDEWIDTRDETFHKRHLIPHLPNYGFDLFEKFFADRTN
                     LITSLLKEI"
     gene            26940..28580
                     /locus_tag="PLG98_05680"
     CDS             26940..28580
                     /locus_tag="PLG98_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOE32779.1"
                     /translation="MIKTSISHPLRIDEVKAPTGTGIIGMTLCPGKKINSALSGVWNR
                     DLDIDLQAIQAWGARALVNLMEEHEYDLLRVPDYVAKVRSCPMEYFHLPIVDVHPPDK
                     RFFDLWESAGPKLRQILLDGGKILIHCRGGIGRTGTVAAQLLVELSMPPKEAIKAVRS
                     ARHGTIETSEQEEYVYTCRPVNERLNIEHFAGCLLGGAVGDALGAAVEFDSIGTIRKN
                     YGENGIENYAPCYGRKGAITDDTQMTLFTAEGLLRAYCREIIRGIPAAYDSTIYRAYL
                     RWLYTQGMKSANEDFTCAQDGWLLTVGDLHHRRAPGDTCLSALQSARMGTPEKPINSS
                     KGCGGVMRAAPVGLFASKALQSAEHQGNEWKKVFDLGCQVAAITHGHPSGYLPAGCLA
                     TIIYGIISGLTIEEAIKKTLTVLKKAKGYEETLDAIKLAMALWKKKMAKPNPETLEKM
                     GGGWVGEEALAISLYCALSAEGDFAGGIRLAVNHTGDSDSTGSITGNILGAMLGKAAI
                     PAHWIEELELKEVIEEMAGDLFVLFGNGDRWRQKYPGF"
     gene            28594..28836
                     /locus_tag="PLG98_05685"
     CDS             28594..28836
                     /locus_tag="PLG98_05685"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HOE32780.1"
                     /translation="MSIHIIPVNKLSAKALKWVIEEFISRNGTDYGAVEASPATSFQQ
                     VKSKLKDGSAVLVFDDETETTNIFMADDPILKKLDT"
     gene            28865..29422
                     /locus_tag="PLG98_05690"
     CDS             28865..29422
                     /locus_tag="PLG98_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32781.1"
                     /translation="MESLPSAQKEPVCIHTFSGIAFDLLDPKPEMILVEDIVHSLSLI
                     NRFNGAAKFPYSVAQHSLYVAGLLPPELKLQGLLHDAPEAYVGDMVSPLKKIMTEYKE
                     VEAGIARVVAEVFGLACPAPPEVKKADLAVLSAEREQVLLPSYGPWYKNFPLPAAIRI
                     EPMSWMQVKELFSSELNALLANRPK"
     gene            29461..>30158
                     /locus_tag="PLG98_05695"
     CDS             29461..>30158
                     /locus_tag="PLG98_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32782.1"
                     /translation="MNTDKFPVVLNALADRNQEIIQKYLREISRSSRTYYIDNKVYNC
                     IYEIAEKEGRKDLAPQSDETLSEWRERAGIPHEYLELEVYLNHLFSDKRRDLLKQPQQ
                     KVCEEDKAQFAELPITDVPAVQSKTDGQGPLGQSKTEPERWGNVYLVVFLLFIFVIGP
                     VVFAFCLESGMGGAGFFIFLVPLGIFIWCHTQGIWRSPTEEEKEAMREEQMRKWGTVK
                     SAHIIRLERGLYTGL"
BASE COUNT         8524 a         6785 c         7320 g         7529 t
ORIGIN      
        1 gcggacaacc catccaggaa aacgacatgg aaaatgcggc ggagatttta ggcgagctcc
       61 aggagacgtc aatcggcgac gttgacgatc agaatctttg ccaggcctgc aaggaagagg
      121 cgggcatgct aagcctgatg gggtttggtg cgtgaagcct gttttgcagg agaagacgga
      181 ttaacgtgag aactgttctt caattaataa tacactttgt tacacaaatc caacacattc
      241 gttttcgtcc atgacgaaag attggagtcc ccatgtccat cctatctcaa ataatacaac
      301 gggacacaaa gaatgcaaag gttgccaagg aaggtgccta tgacaaatta aaaaataccc
      361 atgggcaaaa atttatagat gacattacag gattctatac ggcagccaac aagcaacgtt
      421 ggttacacta taatgcagag cagaaatctt taatagcaat ctgtgaaaat ccgtttcatg
      481 ccatggcaga aattaatact tttcacgaaa atggcagaac aaatcatact atctggtatg
      541 ccaatgagca tacatccata acacaagtca ttgacgatga tatcattatt gttccctgga
      601 cacacccagg ttttcagatt gccatcacag aagatctaaa ccaaaaacat gatgttaacg
      661 acccacgata cagactatct gggataatac ctctcgcacg tgcaagattc cagaaagtat
      721 ttccagtgat cattgggata tacgatccag gtggacatgt tggtcaaata gaaaaagttg
      781 cgacaacaac cggacttaaa gcagtaaagc taaatatgac gaaagaccag gtcgatgcat
      841 tcataagtaa aatgagagga gttctatttg ttacaggtgc acctggtagt ggcaagacaa
      901 ccgtcgcatt tcaacggatg cgttttttat tcgatgaagg agaaacgaga ataggtgttg
      961 agcatcgtcc ggaaaacagc aaggtgtttc tggctaacga aaatttaatc aaacattcaa
     1021 aagatttgct tgaaaagcaa ctagacattc ccccttcaat tgtcagtttg atcaaaaact
     1081 tcatagaaga atatcttgat gatgcatggc ggttcaagca cgaggctttg ttcttgtccc
     1141 gtgatattcc caacgctctc gagcgtcgag cgagggaagc ttttttttcg acatgttcag
     1201 ttgatgatat gaaaaaatgc tggaaaacgt atgaacttca aattgcagag aagatctcca
     1261 ctgcttctca ctctgaatgg gctaaacttc aatatagaag actagaacaa agagaatcgc
     1321 aaagcaacct gataactaga ttagatgaat ttgcgaaaag gcaacaaaaa aggccttttt
     1381 cttctacgcc gacatcttca gaagtcagca tggatcgact ctattggcat tgttcacgcc
     1441 aatatgatga tttgcgaaaa gaattgactc gtccagaggt agaagaattt gatggactgt
     1501 tttcaaagtg gttgtatttc gtttatgatc caattgaatg tcttagaaaa tattttggta
     1561 gtaagaccca tgagggtgaa attcggataa ggcagggcac tggtgctcga ttaaacgaag
     1621 aacttatcgt acgtgacatc cttgaagact gggagaaaag acgataccgt cgggaagaga
     1681 ttgggtggat tgcttggctg ctcagatttg tcttacccac aacggaagat gcaaaagatc
     1741 gttttcgaga gatgcctaat gcctattcac cggtaagtgg caagcatggg ccatggcaac
     1801 atattgtaat tgatgaagcg caggatctgt cagttgttga ggcttccttg ctttcatcct
     1861 ttatactacg cgacggcgca ttaaccattt ctgccgattt cagacaggtt gtgtcacccg
     1921 ttcatggcat gaagaatctt gatgcattca aaattggttg taatctcatt agtaaagctg
     1981 atgatttcac acaatttccg tttactaaaa atttgcgtca atccggacaa attggaaatt
     2041 ttttgagggg gttttacgaa aaagtttttg gtgagttgcc acaatttatg gccaatgatg
     2101 aaatgaaggg accaaaacca caattgcatc tcatgccata tgtaaacttt gcacagacca
     2161 tcagaaaaat gctaaatgta ttcaaagaaa aaaaagttac gggttccatg gcatttctgc
     2221 agatcaatga ggatgaagat gaaatgattc gatatcgcga gatgctggaa aaagaaaata
     2281 ttagcttggc agacatctgg gtgtcggata gtgcggacgg ggcaatggtg acgacaagta
     2341 ttgagaggat taagggacta gaatatgata tatgtttcgt tgtaggctta gagaaagcag
     2401 aaaacacgat gctcaacttc aacaagaatc gtgcctacgt ggctctttca aggcctgcac
     2461 aaaggctcta tatgttctgt gagcatattc ccggtttgct gcacggaatg aacaatgatt
     2521 tatatgatat atttgatgca cgataaggag aaagacattg tttccagaat ttgagaaatt
     2581 cgccaaggaa gagaatcttt tggagataaa gttagccgga aatcttgctg cccaaatccc
     2641 ccggattctt ggccaaggaa aaacgaaagc cataggtttg gaccttccga catcaccaat
     2701 tgagcgaaag caggaatttg tgacccttga cttgtcacca tcattcaata tgccggccgc
     2761 cgttcgtgaa gagcaattgc gcgttaaagc agaactagac agagagatga agcagctctg
     2821 ctctgcagaa ggcgccagaa gcctggcgtc caatgtcatt cgtaacctta acatagaccc
     2881 accggggaat atgaagaaca attactttta tgcgggaaga tttttactga tgcctgacgc
     2941 ccaaagaaca tcttggtcat ggtcaattac accctattac cccattatag aaaaaatacc
     3001 accggatttt gattatcttg taaaagcatt tcccgttgcg gaggaaaagc tcaatcaaat
     3061 cgtaaaacct gtggaggtat ttgaaaagca gatggagctc agttggtggt tggcccggca
     3121 ttttgcggaa gcaagcgaca aggtattact cagcgatgtt gcaagaatgt acgtaatagc
     3181 atcgcaagat aaaagatttt ggaccaaacc tcaaagaagg aactttacgg atgtgccgga
     3241 tgcatctttc atcgccaatt tgatcaacta taaaagcaat tctcctgaac cggtttttga
     3301 gtttatgccg gcaaccctta acgactccta tcggaagaat gtctatcaga taccccttga
     3361 tcgagagggc acgcaaacca ggccctacgt ctatattgtt aaaaaacgct gattatgaag
     3421 acaggaggca aaaatggcag tacttgataa atcagaggct atcagaataa aggcacaact
     3481 catatcaaat ccggttgctc ccggtgtgta ttcgcatttt ataaatgtgg gcacaaataa
     3541 tattattgaa cctttggaag ataactactt ccgaacagaa atgccccttg gtatttcatg
     3601 cttcaaatat ttgcagggct attatggcag cggcaaaacc caatttatca atagccttgc
     3661 ggccagagct tggcagaata acgtggtcac agccatcgtc aacgttggaa ttgactgccc
     3721 attcaactct cctctagcca tctatcagtc tgtcgtcagc aatatgatcc caccgccagc
     3781 tccgggtacc gatccagaag atgagcgtgg tatcgaaatt atttttcgca attatatcaa
     3841 tcaaaaactc aaacaatatg gcatcgaagg aggaaaagca gttcctgccg aggtcagaca
     3901 gctcattgtt cagcccttta cggaaatgta cattggtgct cctgatatgc aggcagccat
     3961 cgccattcag caacttggga aactcatgct taatgtagcc tgcggaggta cagcaacgcc
     4021 ggctgacatg gaaatcattt catggatgcg tggagataat attaccagcc ggcatctcag
     4081 ggatatgggg ctgcataagc cagtgaaaga tgacagtgct tttaatcgct taaaaacaat
     4141 cattatgttc atgacaaaac gtatggggat gaaaggtatt ctcattgcct ttgatgaagg
     4201 cacccggaca gtttcttttc gcaggggcag tgtcaaacaa aagcaggcca tcgaaaatct
     4261 tttgactatg atcaacgatg gggctgatgg caaatttcca ggtgtcatgt ttttgtatgc
     4321 ggcaacgcca gacttccgaa atgaagttat tcaaaaatat cccgcactta atgaccggat
     4381 cgggaatata gctttcaaac ctgggagtcc catggttccc tttatagatc tcgacagttt
     4441 ggatacaagg tcaattgtcc gcgaaatcgg cgctcgtctg ttagaggtat tctccattgc
     4501 ttctggcgtg gagtggaagc agcaaatcca aaaaaacaat attgagcgcc tgatgatggc
     4561 agaggaaaaa agctacggaa tgggttcgga aatacgacga tcatttgtct atcatttctg
     4621 catattcctg ggacaccaga tcaaagaaca atttgaattg tcggataaag acgcggaagc
     4681 attcatttta gatcatcaat tgccacgaac agaagatgag gaggtgtaat gcagctcact
     4741 aagggcgata aagtaacaca ccctgactac ggtccgggta ttgttaatca agtccttggt
     4801 cgcacagtgg gtgtgaattt cgctggtgag gatatggatg tcgatatcag caccctaacg
     4861 cctattggga aaattgcgcc cccagtacca gacaacggaa acgaagatcg gagtcaagcc
     4921 aaaactgaat ttcgcgcggc ttttgaagca atcaatctcg gcgttgcacc acccgatacc
     4981 ggacagctca ttgatttaac cattcggtcg gatgaactca aaaagcgggt ttccggctgg
     5041 cttaccaatg cgccgaaaag cggtttgtgt aaggttgtct ttggctatta tggcaccggt
     5101 aaatcccata tgctcaaatt cgtccgctgt atggcgctga aggcgggatg ggctgttgcg
     5161 tatcttgagt ttgaccccaa ggcggctgat cctgcaaaac cacaccttgt gtatcaaaat
     5221 ttgatgacgg cattggagtt cccaaaaagg gaagatggac gacaaacaga ggactttatc
     5281 ggatttgtta aggaagttcg tgatcattgg gcccagaaaa atattcgtaa taatgatata
     5341 tttaggagta atctctggtt ttcccgcgct tttgaggtat tgatgaaata tccccatatg
     5401 ccggatgtca tggaagatta tcgtgatgcc tgtctatggc ttgctggcaa tcataattcg
     5461 tttcagacag tcaacacact agcacgtgat aagggattga acatcagagt gccccgaatg
     5521 ccgatcacca aagaaacagc cgatatttac gtccatcatc tggttgtcgt gaacgcactt
     5581 tgcaaattac ttggatataa aggactggcc atcatttttg acgaagcaga acatgtgcga
     5641 ggttttaatg tccgaagacg agagcgtgct aataactttt ttgaactatt ggcccgttca
     5701 gcccatcttc ctaatcctga cgatgatgat cccacgatga atgatcacgg tatatccgtt
     5761 ccaccctatt ggaattatgg gccgcacttc gcattgtttg tcggtttgac cgaaggagaa
     5821 acctttttgg atctctcact caacctgaga gatgcttgct tgtttcttcg atcagaagag
     5881 gatcgaatca tgctggccaa tcccagccgg gaagattatg taaaatggtg ccatcttttt
     5941 ctgaaaaagt tctgtagttt ctacgccctt gaaacaacac agcttaaaac ggatgactca
     6001 aaagaaaaga ttgtggattg tctggctcaa aattaccaag cagcacagaa tggtattccg
     6061 cttcgtaatt ttattaaact tgcaagtctt gttccctgca tgcttttgtc tcatcctgga
     6121 atgacctttg aggaattaat ggctcatcta aagacaacaa tggcggagtt tctcggtaat
     6181 gcaatgcctt gggaatagcg aatcaaacct gatgatgcgc tggatgccgg atgcctggcc
     6241 cgtatttttt cacaaacggg gcccgcgtcc tattcaacaa aatgccatcc cactgattct
     6301 caagggagaa tccgtctttc ttaccgcgcc aactgcttca ggcaagacgg aagcggtcat
     6361 ggccccatta taccaacgtc atatttcctt caagcatgat catctttccg tcctctacgt
     6421 tgcccccaca aaagcattgg ttaatgatat ttattatcgc ctgagcgatt atcttggctg
     6481 cggaaaagat ttttcaggaa tctgccgtta cacaggcgat catcatgact tcaaggaacc
     6541 gatgggtgct tttgcgttga ttgcaacacc ggaggcgctt gattctctcc aattacgaca
     6601 tccgcagaaa ctggaacata tcagagcaat ggttgtcgat gaagtgcatt ttatccatgg
     6661 gaaggcccgc ggggaacaac ttagatatgt tatcgatagg gtccgcagta agtccagaag
     6721 cccgctgaat cccaaggatg tattccaggt ggttgcgatg agcgccactt tgaatgatat
     6781 ggaaagcgtt ggtcgtcttt gggtaggcaa ccaggtaaag tatgtcacgg ctgaagatcc
     6841 ccgtgagatc gaaatgcacc atcttcctgt tccgatgggg aaaatacaac aacgggcaac
     6901 ggaaattgcg gcggcaatta gaaattatat tgaaatacaa caaatggaga aggtccttat
     6961 ttttgctaat acgcgtaatg atgcccatca tctcggtttt gttctgcacg atgtcttcaa
     7021 gggaacgcga tggccagtct atctccactt cggcattctg gaaacaaaag tccgcgatga
     7081 aatagaatcc gatctgaaga agaaacgcta tggcatttgt gtctgcacct caactttgga
     7141 gcttggcatc gacataggcg acattgaagc aattatgttg ctgtcaccgc caccttcggt
     7201 cagcagtttc ctgcaaagaa ttggccgtgg tagtcgaagg tcagaaattt gcaaggtcgt
     7261 taccctcgtg agaaatgata acgaacgcgt catatacgaa gcgctgctgg atctggcacg
     7321 gcatggcaag ctggagccca ttcatgaata ttcaaggccg tccgtagcct ttcagcagat
     7381 cctcagtcat gcctggaatg gattaagaaa agaaaaacct ttaacgctga agaatctcgc
     7441 agcaaggacg ggtggacgag actttggtga aattgtcaat agtatgctgg aagaaggaca
     7501 tctgaagctg aaccatgggg cattgatccc gtctgatcgg ctcattgaac ttggtgaccg
     7561 aagtgtcatt catactgtta tcgccggcga cggtgcaaag ccggtctatg atggggtgac
     7621 aggtgatctc atcgcgtcaa taggtgaggg tgccggcgac ggattatact tcctgggggg
     7681 aacgttacgc cgaattgcaa gtggtgaaaa aaacagctat gttctagaac aagttgaacc
     7741 caagggtccc agacagatcg gtaaaatacc ggccgcaaga ggcggacgtg gtatcagcag
     7801 gacgcttgtc tggaaaattg ctgaaatgac gggtcatgat ccgcgtattt ggtcatggac
     7861 caacgataga ttggtaacct ggggaggttg ggtcaacaat ctccttttga aatttatcct
     7921 cgaacagcat ggatatggaa cgatatccag ttttgatgcc tttgccctag aaggaataga
     7981 aattaataaa aaagatgaaa tcaatcctga tgctatttct gcgtttgttc ggaatgcgga
     8041 gattcatctt tctttacggg aagcggaaaa attcagggaa ccgtcaagat tttatcaata
     8101 cctcagtaca aacttaaaga ctaaagaatc tgaaagcgcc atccctgtcc ctgaatttac
     8161 tcagtggctc aacgaatgcc tcatggaaac tgagcaaacc aatgaaatag aacaggagaa
     8221 gactgctttg gcggaagaat gtgccgcgtc ccctcattat gagaatgacg atatggggtc
     8281 attaactgta ttgaaagaat cagtacgcat tcaaacattt tccggcattg cttttgattt
     8341 gctgaacccg aaacccgaaa tgattctggt tgaggacata attcattccc ttgccctggt
     8401 gaatcgtttc aacggcgccg cgaaattccc ttacagcgtt gcacagcatt cgctttatgt
     8461 cgcaagcctg ctgcctcctg aactcaagct gcaaggtctt ttgcatgacg cagcagaagc
     8521 gtatgtcggc gatatggtca gccctctgaa gaaaatcatg accgaataca aggaagtcga
     8581 aacggggatc gccagagtgg tggcggaggt tttcggcctg gcccggcctg cgccgccggc
     8641 ggtaaaaaag gccgaccttg ccgttttgtc cgccgagcgg gaacaggtgc tactgcccag
     8701 ctatggtcca tggtacaaaa attttccgag tccggctgac attcgcatag agccgatgtc
     8761 atgggttcaa gttaaagaaa tattttcatc tgagcttcac tccctactgg agagtcctct
     8821 tgaatagtca taaagataag tgtatgaaaa catgcagtaa ttgtggacaa ccgattcagg
     8881 aagacagcag caagacccca gcaaaaatcg ttgccattat tgttatggtg agcttttcga
     8941 taaactcagg acgggcacta ttgaaccatg atctttcagc cggtaaaggg tgacattgct
     9001 tgtcgaaaca ttaggagaag acgtaatggc tgttatgatc ccggatgatg ttcaagactt
     9061 ctgcacggaa ggtgaacgtc acttctacgg atttctgaaa actgccgcca aacccgatag
     9121 caaatatctg gcctggtacc tgccggacat caatggccgt gaacccgatt tcatcctgtt
     9181 ctgcaaagac accggtctca tcatctttga agtcaaagac tgggcgctcc atcaaatcca
     9241 ggcagcaaat ccccagaatt tcactttaac gatcaatggc cgtaacgagg aaagagaaag
     9301 ccccttaaag caggcgagaa cctattgtca tatccttatg gacaagatcc gcgatgaccg
     9361 ccgcctgctt tccaaagatc cccagcatgc aacaaacccc aagatcccca tccatgaagg
     9421 cgtcgtgttt cccaatatca acaagtacga atacatccag aaagcctttg ataaagtcat
     9481 ccattcggaa caaatctttt tctgggatga tctgcacccg gaatcggata tttgccgcga
     9541 cctgtccggc cagtgctttt ccacagtcct gcagaagaaa tatgccgcac cttttcattt
     9601 caacctgaca ccggatgaaa tgcagcatct ccgccagctc ctgtttccca tgatccgtat
     9661 tgaactgccc gatcgtacct ctaaagcaca acggcagagg gagaaggaca ggatcaggct
     9721 gctggatcat catcaggaat ccattgcccg taagttcgac ggcggccacc ggattattac
     9781 cggcccgtcc ggttcgggga agacacttgt tctggttcac agggcagccc tccttcaaca
     9841 atacaatccc gccgtcaagc gcatcctttt tgtctgctac aacatcaccc tggtgaattt
     9901 cattaaaagg ctcctttccg agaaacaggt tcccctgggc gaaaacggca tagaagttgt
     9961 tcattttttt gagttgtgtg caaagatttt gggcgaatcc gtaacctggg agaaggaaga
    10021 accggcttat tacgacctgg tcattgaaga gacgctgaag aaagtaaagg attatcccga
    10081 aaagtatgat gccgttctgg tggacgaagg ccaggatttt tccgacgaca tgtactccat
    10141 cgtcgtttcc cttctgaatc cggcaaccaa tcatctggcc atcgcccttg acgacaatca
    10201 gaacatctat cgcagaaagc agacatggaa agaccttggc attcaggtcc gtgggcgtgt
    10261 ccaccgatta tcctgggttt accggaacac cagcgccata gtcgactttg cccggaaatt
    10321 cctcaatgaa aaagaagaaa aggccgagca cccgccacat cagatggaac ttttccaaaa
    10381 cttcctttcc ccggcaggga cggagccgga aatccggcaa tatccagacg tggatgaatt
    10441 gataaaccac gttgccaagc aaattaatca tctgcataat gacgatggct atccactttc
    10501 cgagattgcc attatctata cacagaaatc acccgattcc ttacccggtg ttcattttcc
    10561 tcatctgatg atcgaggccc tggaaaagta tggcatttac tgcaactgga ttgccgaaga
    10621 ttaccgtgcc aagcgctctt acgatgtaac gacacagagc gtaaccatct ctaccatcca
    10681 cagtgtcaag ggctttgatt atgcctgcgt ctttgtcgtt ggtctggact ggcttcaagg
    10741 agatcgttgg acggaagaac aggtgaagaa tctgacttat gtggcgatta caagggcaag
    10801 ggagaggttg tttgttccgc accttcatga tttgatactg ccgaaatgag aatggttttg
    10861 tagccgctca gaacaaggtc cactggaaca acaaactaaa tgagatattt tattggattt
    10921 ctaaaatgct tttaccgaga ttaacaacct atcatacgct tgaaaacgga agggaactca
    10981 tgattcatgg ggtttatgtt ctattggcaa tattttacat aaaatatgga gatgattgat
    11041 ggacgttttt gcgttaagga atagattggt tgctgattat cgcagctata cccgcagctt
    11101 tataaaaatt gccgaccagc gcatttctga attagttgat acgaacctta ctgcaggtgc
    11161 gttttggcct gaacctttgc tgcagctcaa tcctattttt aaatcaggcg gcacaatcga
    11221 tgaactggtt agaaagaatg tgctcaatcc cgaatgtgcg aaaattttca gaattgataa
    11281 aacggataca gattttactg gcaagcagct caacctccat acgcatcagc gtgaagctat
    11341 tctgaaggca aaggaaggca aatcctatgt gctgacaagc ggaaccggct cagggaaaag
    11401 tcttacctat atcgtcccga tagtggacca tgttttaaaa aatggaaccg gccgcgggat
    11461 acaggccatt gttgtctatc ccatgaatgc ccttgcgaat agccaggatg aggaactggg
    11521 gaagtttctt aaaaaaggat atccggaagg aagatcaccc gtcacattcg cgcgctacac
    11581 agggcaggaa aaaggcgaag aacgcgaagc aattcgtcag aatccccccg atattctgct
    11641 gacaaattat gtcatgctgg aacttcttct gacaagaacc gaagatcgtg aactggtgag
    11701 ggccgctcaa ggattaaaat ttcttgtgtt tgatgaattg cacacatata gaggaagaca
    11761 gggcgcggat gtcgcgcttc ttatccgacg ttgtcgcctc gcgtttggcg gacagaatat
    11821 tatttgtgtc gggacgtctg ccacaatggc cagtgaaggc acggtggaag atcagaaaaa
    11881 ggccgttgcc agtgtagccc agactctctt tggtgtaccc tttgaaacat cgcaggttat
    11941 aggggaaaca ttatctcgtg cgacaaatga attggaccat aatgatccgc aaatcgttca
    12001 attattgcga aaaacaatcg aagctgacga ggcaccaccc gctgattatg agagttttat
    12061 caaacatccg cttgcttcct ggatagaatc cactttcgga ataaaaactg cagagggttc
    12121 tgatagactt ctgcgccagt tgccacgacg gttagaaggc aatgatagca cagcggaaga
    12181 gttggcaggt cttacttcaa ccgatgaaca aagatgcgcg gaaatattgc gtcgttattt
    12241 gcttcagggg tcctatttaa aagcaagtac atccagtcgg tttcctgttt ttgcgtttcg
    12301 tctccatcaa ttttttacac gcggtgatac agtctgggcg acgatagaac ctgaagccga
    12361 ccgccatttg gaaatgtcca aaaaggtttc caaacccggc gaacctgaaa aacccctcta
    12421 cccgctggtt ttttgcaggc agtgtggtac cgcttactat cgagtcaaaa taatctccaa
    12481 taacggggat aaatatcttc tcccgcggga ggaaacaagg caggcggatg atgacgaaag
    12541 cacattcggc tatctttatt tatctgaaac ttcgccatgg ccacgcggct cgggcccgga
    12601 acttctcgga cgtgtgccag atgctcttaa ggaaactaat ccgcgaggag aagaacgaat
    12661 aagaccggac gcaaaaaacg atatccccga aacggtttat attgatgcaa caggaaaagt
    12721 atgcgcagag gggcaaggcg ttgaagcagc cctgattcat cgcaattttc ttttctgtct
    12781 tgaaccatcc tgcggtgtcg cctacacgag aagccaaaag gcagaacggg gaaaactggc
    12841 cacacttgga attgataacc gcagcacggc aacgaccatt cttgccgttc gttcattaat
    12901 tgaactgcag ggcgacaggg atcttgaggc caaagcgcgc aagcttttga gtttcacgga
    12961 taatcggcaa gatgcctcat tacaagcggg tcactttaat gattttgcac aggtagctct
    13021 attgcgttcg gcactttaca aggctgcaca ggcaagagga gaagaaggcc taaaacatca
    13081 cgatctttca cgcagcgtat ttgatgccat gcaattaagt ttcgacgaat atgcggttga
    13141 tttggaagtg cgcggtcccg caagaaatgc aacaaacgac gccctccgac gagtcataga
    13201 atacctcgtc tatcgtgatc tacagcgcgg ttggcgtgtg acggctccga atcttgaaga
    13261 ttgtggcctt gtacgctttg cttatgaagg tttaaatggc gaggaaagcc tgctgtccga
    13321 acaaaggctt tgggatgaag gtttttccat tcgaacagat aatggtgaag agtttctttc
    13381 cataccagaa gcattgcgct tgtgcacacc ggagaagcgt gaagaaataa tcaggacttt
    13441 acttgatgtt cttcgccgtt cactggcgat aaaagttgat gtgctggatc ctcaaaaaca
    13501 gtttgatctc gtagaacaga caaagcctcg tcttcgtgaa gatacagtct ggtacctgga
    13561 agatgggcgt gatctaatca aggcgtacgt cgcttatccg cgtgcgagaa taagaaacaa
    13621 ccgttacgaa gaatacaatt tcctgcccgt ttcctcatat agtggttatg gtagatattt
    13681 aaaaagaact ctgaagaatt gtgtcccccc gggcaggcct ttcggacgac aagaagtgga
    13741 tgaagtaatt cgatttttgt ttttggcatt aaaacgttat ggaatcgtcg aacaagtacg
    13801 aagccgtccg gatggctctg atcccggtta tcagataaat cctgcggcaa ttatatggct
    13861 gcctgccgat ggtgagatac gcccgataga ccgcacacga cttttggatg ccggagaact
    13921 gccaccggaa gtgaaccggt actttgtaga atgttatcgg cattttgttg acctgaaatg
    13981 cctactggaa gcgcgagaac acacggcgca ggtcaaggcg gaagaccgtg aaaaacgtga
    14041 aattagattt cgtaaaggtg ccgagccggg tggcctgccg cttcttttct gctctccaac
    14101 catggaactc ggcgtggata tcgcgcagtt gaacctggtc aatttgcgga atgtgccgcc
    14161 gacgccagct aattatgccc agcggagtgg acgcgccggt cgtggcgggc agccggcact
    14221 cgtttatacc tactgtgcgg ggcgcagccc tcacgatcaa tatttttaca gggaacccaa
    14281 ccagatggtt tccggttctg ttgtcccgcc gcgcattgat attagaaaca gagacctggt
    14341 aaaatcgcat atccacgcag tgtggatgga agcggcaaag ccggaccttg gaaaaacgtt
    14401 aacgcaggtt atcgacctga cacctgcaga tggcagactc ccattaccga tcaaagatga
    14461 aattttaaga gaactaagaa gtcccgttca tcgggcaacc gcgcaaacaa gagcaaatca
    14521 gcttatagaa agcatccaag atgaactaaa taacgcgccc tggttccatg ataactggac
    14581 gaaggaaata cttgatcaaa tcgaacgcac tttcgatgtg gcctgcgagc gatggagaag
    14641 cctttaccgg gcggcggttc gccagagaga aattcaccac cgaattatcg gcgatcacgc
    14701 gcgaccggag atggaacgga accaatcacg ccgcttgcgc gcccaagcgg aaagtcagat
    14761 taaacttctg acggaagcgg aaggtattta cgagggtgac ttttattctt accgttactt
    14821 tgccacggaa ggtctactac cgggttataa ttttccccgc ctgccgattt ccgcctatgt
    14881 gccggggcac agacaacgca acagtcggga cgagtttgta tcccgcgctc gttttctggc
    14941 tatttccgaa tttggcccgc gcgctctcat ttatcatgaa ggtgcccgtt accgtgtata
    15001 taaggtcaat ctcgattttg gtactgatga tatcgaagag acgcacaatc tggtaacagc
    15061 gacaatgaaa cgttgtccag tctgcggtta cgctcatatt gaacaagata gaaatttaaa
    15121 tgagatctgt gacaattgtg gaggtgctct ggacggttct tcgcaaatag acaatctgat
    15181 tcatcttcag aatgtcagcc ttaaactggc ccagcgcatt acgtgtgatg aagaagaacg
    15241 acagcgtttc ggctaccgga tcgtgagcgc ttaccgtttc cccgaaatcg gcggcagatt
    15301 ggatcgtcgg gatgccgagg tatattgtga aggaacgctg acgctcagac taaaatacgg
    15361 tgattccacg gaattattcc gcgtcaatct aggttgggca aatcaaataa tcggacagcc
    15421 gatcggattt aaccttgatc tcgaaagagg ctattggtgg acaaacaggg cggatgcgga
    15481 agacttgcag gatgcgaaca cgcaggggcg atctcaaaga gtcgttcctt acgtgagaga
    15541 tacaaagaat gccctggtca tgtcttttgt accggttcga ccactgccgg aaaccgccag
    15601 cttgcaagcg gcttttaaag aagccatcca gaaatatttt caactagaac cgagagagct
    15661 tgaatgcgtg cccatgccgt cgccgcaagg acgtcgcgaa ttgctattct ttgaatcatc
    15721 agaaggcgga gccggcgtgc tgcggcaggt cgccgaagat ccgtccgtgc tgccgaaact
    15781 tgcccgcatc gcattggaaa tctgtcattt tgatccggat actctagagg ataaaggcgc
    15841 gttgacctgt ggcaaggcct gttattcctg cctgctcgat tatggcaatc agccggatca
    15901 taaagatctg aatcgctatc tgatcaggga tttgctcgcc cagttttcga agtccgaatg
    15961 caaaccatca ggtggaacag gcaccagggc cgaaagaatg aacgcgctgc gaaaacgatg
    16021 tgacagtcaa ctcgaaaagc gttggctcga tatggtggac aggcttatgc tcaatcctcc
    16081 gagtgatgcc caatatctga ttgaatcctg ttcaacaaaa cccgactttt actatcgcga
    16141 ttttaatact gccatttaca ttgacggccc gccgcatgat gagccggagc aaattcgtaa
    16201 agatgaagaa ataaccagaa agcttatagc aaacggttat attgtgatcc gcttccatca
    16261 tcgggtggac tggaacgcaa tatttaatca gcatcccgat atttttggga ggcctcaaac
    16321 atgagcactc ccaatcttgt ccggccggga acactaatta atgccagagg aagggagtgg
    16381 gttgtccttc cagagtcttc tgaagaactg ctgctggtac gtccggtggg cggcctggat
    16441 gaagaattcg ccggtatttt accggcgatc gaacccgtag aatccagctc attcccattg
    16501 ccaactcttg aagatttggg tgactttaca tcaggcagaa tgctgcggga tgccgcaagg
    16561 ctttctactc gtgctgccgc agggcccttt cgctctttcg gtcgcattgc tgccgagccc
    16621 aggccctacc agcttgtccc cctgatgatg gccctcaggc ttgatcccgt gcgcctgctc
    16681 attgccgatg acgtgggcat tggcaagacg atcgaagcgg cgttgattgc ccgtgagctt
    16741 ttagatcgtg gagagattaa cagctttacc gtgctctgcc cgccgcatct ggctgaacaa
    16801 tggcagaggg aactttcaca gaagtttcac atcgatgccg aactggtgct tgccgaaacc
    16861 attcacaggt tggaaaatga tcttccggtt ggcgtttcca tatttgacag gcatcgtttc
    16921 acagttgttt cgacggattt catcaaaagt tcacgtagag cggatgactt tattattaaa
    16981 tgcccaaagc ttgtcattgt agatgaagcg cacggatgca cgctggctga aggagtcgga
    17041 cgcggacgac agcagcgctt tgacctgctg aagcgaataa ccgaagacga tgatcgtcac
    17101 ctgattctgg tcacggcaac accgcatagt ggaaaggaag aagcctttcg ttcactttta
    17161 agtctcttaa gagatgaatt tgccaatctt cccacggatc tcgatcgcga agagcgtgcc
    17221 gacattcgcc gcaaacttgc acggcatctc gttcagcggc gaagggccga tctccgtcac
    17281 tatctggatg ctgatacaag ttttccggaa cgttatgata aaaatcccga accgacatac
    17341 accttcagca aagaatataa acagcttttc gacgatattc tatccttcgc ccgcgaatac
    17401 gtttcagagg atggtacatc cagaaggaat cagcgtgtgc gctattggtc ggcgttggca
    17461 ctccttcgct gtgtatcttc aagcccggcg gcggctgccg caacgttgcg cagcagggca
    17521 gccgttgatg aagccgcgga tatcgaaata gacgaggtgg gaaggcggac cgttctcgac
    17581 caggatgata cggacgacgt ggctgtcatc gacttcagcc ccggttccga cgatgaaaca
    17641 agtgccacaa acacaagacg caaacttcta gaatttgccc gtcgcgccga atccattccg
    17701 acggaagcag acagaaagct tcagggagcc atcaaagaga taaaagaatt gctcaaggaa
    17761 aaattccacc ccattgtttt ctgccggttc gtggatactg cggattatgt tgccaatcat
    17821 ttgcgcgagg ctttgggcga aaagattcgt gtccaatccg taacaggtct tctgcctcct
    17881 gccgagcgtg aagcccgcat agaatcgctt gtctccgaat ctgaaaaata cgtgttggtc
    17941 tgtacggact gcctggcgga aggtgttaat cttcagaatg atttcaatgc cgttttgcac
    18001 tatgacctgg cctggaatcc tacccgccat gagcagcgag aaggacgagt tgaccgcttt
    18061 ggacaaaaaa gtctgcaggt gagagtgaca acttactacg gcaccgacaa tcccattgac
    18121 ggcgttattc ttgatgtgct tatccgcaag cacaaacgga ttaaaagtga tctgggtgta
    18181 acagtagctg tgcccggctc gagtgagcag atcgccgagg ctctttttga aggggcttta
    18241 ttccgggaaa agacccacgc gcatcccgac cagttgcgtc tagatttcat cgatgaaatg
    18301 gagacaaaaa agaaagacct tcatgtccag tgggataatg cccgcgaccg cgaaaaagca
    18361 agccgttccc gtttcgcgca gcatgctatc aatcccgaag aagtggcgca ggaacttgcc
    18421 agtgtccgtg ccgcgattgg cagaagtgaa gatgtttccc gctttttcag tgacgtgctt
    18481 cagcttacag gtgttccatt tgaccaaaaa gccaatgcgg tgcgcgtcca tgtcagcagt
    18541 gaaacgcccc gtgccttgag gcaggccatc ggcttcgatg atccctttgc cggccgcttt
    18601 gagttgcctt tgggggaaaa ggaaatttat ctggcccgta ccagtcctgt catcgagggt
    18661 ctggccaatt gggtgcttga ccaggctttg gatcctgtcg cgcgcgatgg ccgccctatc
    18721 gccgcgcgtc tgggtgttat ttccacgtca gccgttcatg aacgaaccac cctttttgtt
    18781 attcgtttcc gctatcatct ggaaaaagcg gggactgatg aggccattct ttgcgaagaa
    18841 gttgtcccgc tggcatgtac aggaccgcag gataatccaa cttggctgcc gcaggacgaa
    18901 agtgagaaat tgctttcggc aagaccggaa cgcaatctga ttgcaacggc aatcaatcag
    18961 caaacggggc ttttacaaga attgctgccc gcattccggc aatctctgga aattgtagcg
    19021 aaggaacgtg cagacattca gcttgccgct catgagcgtg tccgtgccgc actcaaaaca
    19081 aaaacccgca tggcgataaa accggttctg ccggccgaca ttttgggcgc ttatgttgtt
    19141 cttcctaggt tgatctgatc atggcaaaaa gagaccagac ctttcaatcc atccgttccg
    19201 aaggcgggct tcttccaccg gatttactgc ggcgcatact tgacccgaaa ggaaaaatac
    19261 ccggaacgct tcccgaagat tacgggcttc cgaaaggcga acgtcttaac gaagtcatta
    19321 cccagtcctg gaaccgtttg cgcagacatt gggctgagtt cactgaagcg atagaaaagc
    19381 tttcgtcgtc cgataccggt acgggaatta ccaacgaaaa gtggtcgctt cctctgctgc
    19441 gtgaactgga ttatggcacc ctgccggcaa gcgccggtcc cgaaatcaat ggccgtactt
    19501 acgccatcaa ccgctttgcc ggtccagcgc cggtgcatct tatcgggtgc aagctctcac
    19561 tggatcgccg tgccgccggt gtccgcggag ccgctgcagc caatccgcat ggcctcgttc
    19621 aggaatattt aaaccgaaaa gaagaaagtc tctggggcat cgtctgcaat ggttacaagc
    19681 tcaggatttt gcgcgacaat ctggcactgt cccgccagtc atttctggag ttcgacctgg
    19741 agaccatgtt tgccggagaa ctctattcgg actttgtctt gttgtggctg gttgcgcacg
    19801 cgacacgctt tatcccgaag gtggaaggaa gagtcgagac ctgcattctg gaggaatgga
    19861 caaagcttgc cgaggagcag ggtacacgcg ctctgggcga actgcggacc ggggtggaaa
    19921 aggcgcttca gatactgggt gaaggattta ccagccaccc taaaaacacc gacctgcgcg
    19981 atgccctgcg cacgggaaaa ctttcgcccg ccggtctcca cggccagctc ttaaggattg
    20041 tctatcgtct gattttcctt ttcgtagctg aagaccggat actcgaaggc atgcccattc
    20101 ttcatccccg tgatgatgca gaggcggccg gtagggctcg tgaacgatat gccgcgcatt
    20161 acagcacctc acgactgcgt gaccttgccg gcagaataaa gggcagccga cacggcgatc
    20221 tctggcgtca gtttcagatc atcgtccatg ccctttccgg tgaaaaggct tattccgcaa
    20281 cccgcgaaaa gctcgccctg ccagcgctgg gaagctttct ctggaaccct gcatccacag
    20341 ccgatctcaa tggggcggaa ctcatcaatt atgattttct ggagtgttta aggcatctgg
    20401 cctttacccg acaggataag attctccggc cggtggacta caagaacctc ggcgccgaag
    20461 aactgggcgg cgtttacgaa agcctgctgg cgctcagtcc gcaaatcagc gccgacggcg
    20521 cccgctttac ctttgccgaa tttgccggca gcgagcgcaa gatgtccggt tcttattata
    20581 cgccggattc gctggtgcag tgcctgctgg attccgctct cgatccggtg gttgaagaag
    20641 ccatcaaaaa taaaaaaggt aaagatgctg aagactcgat attatcactg aaagtctgcg
    20701 atccggccgt cggctccgga cattttctgg taggtgcggc gcatagattg gcgcgccacc
    20761 tggcccgtgt ccgggcgctg gctgaagggg agagcgaacc atcgcccctt ctccaccagc
    20821 aggccctgcg ggatgtgata ggacattgcc tttacggcgt ggacatcaat ccgatggcgg
    20881 cggaactttg ccgcgtcagt ttatggctgg aggcgctgga gcccggaaaa ccgctgacct
    20941 ttctcgacca ccacatacga ataggcaaca gccttctcgg tacaacgccg gaactcattg
    21001 ccaaagggct tcctgaggag acattcaatc ccatcgaagg ggatgacaaa aaaatctgca
    21061 cggccctgaa gaaacgaaac aaacaggaac gagaaggccg ccagacggac atgatgcacg
    21121 ctatggttgc ggaaagcgct gagcaatata acaccttgga agcacgcagc cgctcgattg
    21181 acgatgcgga ggacgaatcc attgaagata ttctttataa gacagaacaa ttccacaaat
    21241 tgatggcttc acctgaatat cgtcgcgcaa gacttctggc cgatgcctgg tgctctgcat
    21301 ttgtctggca caagcaaaag ggagtccctg aacccataac aaccgatacc atcagaagac
    21361 tcaatgacaa ctccgaatcc ctttcgcaat cccaggtcaa agagatcatg cgcctgtcgc
    21421 gccaataccg cttcttccac tggcacctcg cttttccgga agtatttgcc aatggcggct
    21481 ttgactgtgt gctgggcaat ccgccctggg aaaaagtcac gacgctggaa agagagtttt
    21541 tttctacaat agcagaaatt gcgcaagaaa aaagaagcga taagcgaaaa gctaaaatat
    21601 tattacttcg agatactttc cctgaacgct tcaatcaatg gattgaacaa ttgagatatg
    21661 atgcagcaca aatgcaattt ctaagaaata gtaaatattt tccactttct gcagtaggtg
    21721 aattaaattt atatcccctg ttcgttgaat tggctgagca tcttattaga gaaactggta
    21781 cggttggtat gattattaag acaggtatga tgttatcgcc aacatgggct gatttttctt
    21841 cttatttaat tgataatgat cgcattgact ctgcctttga cttcagaaac tggaatggct
    21901 ggttcccgga tattggatat catgaacgtt tcagcttgct gaccatgaaa aaagggcgtc
    21961 gaaagcgaga tattgtgctt ggctattacc tcgatgacac aatggagata aaagattcac
    22021 caaagaagtt taccatctcg cgggatgagg ccatccggct caatccaatt accaaaacgg
    22081 ttcctgcttt tgaaagcaga caacacaaaa cctgcgtcat ggcggtctat gatcgctttc
    22141 ccgtcctcgg agacaaagct tcagaatggg gggtgcatta tactacaggg cttcatatgg
    22201 ctgccgaagc ggacaagctg cgtgattttg aagagttgtc tgcggaagga tttgcgcccg
    22261 tagaatttca gtggatgaaa aaggaagtgg tcacctttgt ccccttgtat gaaggtaaat
    22321 tcatccacca gttcgatcat cgtttcgcct cgttcgaggg gattctgcgg gaaaaacgtt
    22381 tcggcattaa agccgggacc catacgccct cgacggatca gaaaagcaat cctgcctatc
    22441 acatcatgcc caggtactgg atatccgaag atgacgcggc gtccgatagg gaggcgcgag
    22501 atttgcggag cagctgggcc attgcattta gagacactac caatgtgatc tcaaactttc
    22561 gcaccagcgt ggcctgcatt tgttcaggcg tggctttcaa ttacaaggca cccaatattg
    22621 tgttgatggg accatcatca caagaaaggg caagggcctc cctgctattt gtgaccctga
    22681 tgaattcttt ctctttcgat tacgtggtgc gacaaaaatt tttcggagca aactttataa
    22741 agtccattct cttgcagctt gccgcgcctt ctatgacaga aatccaacgt catcagcaat
    22801 ttattctgcc tagggcgttg gagttgtctt atacctcttg gcagttggaa ggtttttcca
    22861 cggaatgcgg ccacaagggg ccgccgttca tctgggatga catgcgcaga cagcaaatca
    22921 gggcagaact ggatgctcta ttctttcatg tctatggttt ttctgtcgat gaagctgcat
    22981 ttattctgga tagttatcgt attttacgag ataaagacga agaagccttc ggggagtatc
    23041 gcacaaaacg actgatcctc gaaatctacg acgagatggc cgaagccatc cgcaccggca
    23101 aaccctatca gacccgtctc gacccgcccc cggccgaccc ccgctgctgc catccaccac
    23161 gtgaaagaaa ggagacagaa gagtgaaaaa taaaacaaag caagataaaa tcattacgaa
    23221 gcgccgccgt atatctgtcg ttttaaatga catgctggcc gggaaatacg cgatacctcg
    23281 attgcagaga gaattcgtat gggacggaca aagagctgca aaactcatgg atagcattta
    23341 taaggagatg cccatcggaa cttctctcat atgggacact actaaatcta aaaagcttta
    23401 cctcagacag cagtataacg ttctgcctca tttcaacgac aagaacaagc gcacctggtt
    23461 tctaattgat ggacaacaga gaatgtctgt cctgcatggg attcatcaag gaaagccgat
    23521 aaaaaattca aagggtaaag agataaattt caggaacatc gttttttctc tcgaaaaaaa
    23581 gaaaaaatct cctgatggaa aggaaataga gcaaagaata ttgtaccgta aaccacaaca
    23641 gggccgattt gtttctctaa gcgatattct ttctccgcaa ttcaatcatc acttcaatgg
    23701 attgaaaaag agtagtctga gtgccttgaa tgaatgccgt aaacgcataa tgaattacaa
    23761 aatagtgttt ataagtacgc gcatgtccga tatcgatgag gttaaggaat gttttctccg
    23821 cattaatacg cttgggatga aagtatcgtc ggcagacgca atatttacaa gagcccaaac
    23881 gttggattta agggatttcg tacatgaggt tagaaattgt atcgacaatt tcaaggatat
    23941 agatgagatg cctatccttt gggccatgac tgcaatacga ggggaatgtt atccgggcaa
    24001 gaatagaatt gacgctgcgg tcaacaagat tgaagaaaaa gctcaaaaga aaaatcatgt
    24061 aaaaaaagaa ctaaacaaag aatggagttt actgaaagag tgcttcagaa agtccgcagt
    24121 ttatctggaa aatgagttcc gtgtcttgag tgccaattat ctctattcgc catatatgct
    24181 gtccatgctt gcattatttc attatttcaa taacaaaaaa agtccaaatc aaaagcaaaa
    24241 gaaacagata cgtcgatggt tctgggtaac gactacagca aggaattatt caggtcagtt
    24301 attctacaaa tctatccaat atgattcagg tttctttagg agattagcga aaaatgataa
    24361 cgcacgcttt cctttgtcta cagatgccga ttttcgtgac atcctaagca cgccgtatga
    24421 taaaacaaaa tctggaatgg gcgcctcttt ttatagtctg cttttcaaga aaaagccggt
    24481 tagccttctg gatgacgcac ttaatgaaat acaactggaa agatacctta cccctgtaaa
    24541 caaggatcgt catcacatat tccctcgatc attacttaaa aaaataggca ttagacagac
    24601 tgaatacaac agtatttgta atatttgtct tctcaccgca gaggaaaacc agagcattgg
    24661 taataagcat ccatcgaagt atttgaagga agcaaaaaga aacaaaaatc tttttggtaa
    24721 aaagatgtcg catcatctga ttccatcttt tgacaataat caaggtttgt gggcaaaaaa
    24781 tacaaagaag ggctttcata aattcatcaa agaaagacaa atactgatca aggaaagtct
    24841 tgagaaagag gctggaatta gactgtttag agttgacaaa taatgtaaaa caactggcaa
    24901 ggttctaata tattacaggc agaattattt tattgataaa taattaaaga agaaaggagt
    24961 aatatggaat atattccaat tcgcgttagt gacattatcc gacaggtaaa tcatgatata
    25021 tttctaccag cgattcaacg agaatttgtc tggaaaaccg atcgtattga aagattattt
    25081 gattctataa tggctgattt tccaattggt tcctttttgt attggcgttt agaacaaaaa
    25141 aataaagata tgtggccaat atatgaattt attcgcgact ttgatgatga tgcaccacat
    25201 aatgctccag caaacatggc tggcattaat aaggatataa cccttgtttt ggatgggcag
    25261 caaagaatta catctctttt tatgggactc aaagggtcat ataaattctt ttattaccgt
    25321 actcatgcca cgaaactgta tttaaacctt ttaaaaaatc cactacccaa tgaagacaat
    25381 ccggaagagc ttacctatgg ttttgctttt agagaaaatg cagagcctga tgataatgat
    25441 aatcctcaat tatggtatct tgtcggcaag atattgaatt atgaagatgc agaagatgct
    25501 aaggctgata tgaagacaga acttgcagga ttgacggagg aacaaagaga aaatgccaac
    25561 aaattaatag gccgccttca taacagaata catacaactc ttgttggtaa ttactaccaa
    25621 gaaaattctc aggattatga taaagtatta caaatattcg tacgggcaaa ttctgctggt
    25681 cagccgcttg aatattcaga tttactcctg gcgactgcaa ctgcaaaatg ggagaatctt
    25741 gacgcacgca gtgaaattca tgactttacc gattcaataa atgaaatagg tacaggatat
    25801 aattttggga aagacttcgt tttgaaagca tgtttatatc tcagtgagat aaagaaggat
    25861 gacgaaatat taggattacc aattcaatat aaagtgaaaa acttcacaaa gagtaatctg
    25921 cgaataattg aaaataactg ggaagatatt aaaatttcac ttttaacaac tgtgagactc
    25981 atctctcgtt ttggtttcaa ttctaaaaat gttgttgctc cactggcgtt attgccgatt
    26041 gctttctatt tgaaaaagcg tggcaattca tcgttcgaca catcttccag tgttaatgat
    26101 gcagatgcgc aggttgccat acgaaaatgg tttatatttg ctacgctaaa aaatgcattc
    26161 ggcggttctt cggacacaac actaacaaaa ctaagagatt tgctaataaa ttatgactca
    26221 aagatgacat ttcctgcgga cgtattatat aaagcacttg gtattgagcc gcatcttcaa
    26281 gactctgaaa ttgaaaaaat tcttggttat ggttatcaag gacgatacac aaaccttata
    26341 ctctcacttt tatatcctga tagagattgg aaagatgctg ttttccatga agatcacatt
    26401 tatccaaagt cagagttcca agtcaggctc ttaaagaagc gtggctatga tgaaccaagg
    26461 atccaatcct atattgcaaa gtacaacact atcgcaaatc ttcagttgct tacagaatcg
    26521 gaaaatctgt cgaaaaacgc cacactcttt gacgaatgga ttgatacgag agacgaaacc
    26581 tttcacaaac gtcacctgat tccacatcta cctaactacg gatttgattt gtttgaaaaa
    26641 ttttttgctg accgcacaaa tctgattaca tcattgctca aggagattta gcgatctata
    26701 agcttcgcat tatataacat aacttaacta aacaagataa aatataagca ttccacgttt
    26761 caacatgtta ttcggttcca ttttagaatt aaaatgtggt tcaagaacaa gcttaatgat
    26821 gtgattaacc acttaaaaac cctttgccaa tgctcgagag ttttgaggga taagtattag
    26881 agtgaaaacc agtttacgat tcagtgccat gtaatggcaa gcaaaagaaa ataataacca
    26941 tgattaaaac aagcatttcc catcccctgc gaattgatga agtaaaggct cccacaggca
    27001 ctggcatcat cggaatgaca ctgtgtccag gcaagaaaat caacagtgcc ttgtccggcg
    27061 tatggaatcg tgatctggat attgatctgc aagcgattca ggcgtggggt gcacgggcgc
    27121 tggtgaatct gatggaagag cacgagtatg atcttttgcg ggttccggat tatgtggcaa
    27181 aagttcgttc ctgtccgatg gaatattttc atctgccgat tgttgatgtt cacccacccg
    27241 acaagagatt ttttgatctc tgggaaagcg ccggtccaaa actgcggcaa attctgttgg
    27301 acggtggaaa aatcctgatc cactgccgcg gtggcattgg ccggacaggc acagtagcag
    27361 cccaactgct ggtggaactg agcatgcccc ctaaggaggc catcaaggcg gtgcgatccg
    27421 cccgccatgg aaccatcgaa acaagcgagc aggaagaata tgtttatacc tgccggccgg
    27481 tgaacgaacg gttaaacatc gagcattttg cgggttgcct cctgggcggt gcggtaggtg
    27541 atgcccttgg cgctgccgtc gagtttgatt ccattgggac catccgcaag aactacggtg
    27601 aaaacggtat tgaaaattac gcgccttgct atggccgcaa aggtgccatt acggacgata
    27661 cacaaatgac acttttcacg gcggaagggc ttttacgtgc ctattgtcgg gaaataatca
    27721 gaggcatacc ggcggcatac gattcaacga tttaccgtgc ttatctgagg tggctatata
    27781 ctcaaggaat gaaatcggca aatgaagatt ttacctgtgc gcaggatggc tggctgctga
    27841 cggttggcga tctgcaccat cgacgcgcgc cgggcgatac ctgcctttcc gccctgcaaa
    27901 gcgccaggat gggaacaccg gaaaaaccga ttaacagcag caaaggctgc ggcggtgtga
    27961 tgcgggccgc gccggttggt ctgtttgcgt ctaaagcttt gcaatctgcg gagcatcaag
    28021 gcaacgaatg gaagaaggta tttgatctgg gctgccaggt cgccgcaatt acgcatggtc
    28081 atccatctgg ttatctgcct gcgggatgcc tggcaacgat catttacggc attatttccg
    28141 gtcttacaat tgaggaagct atcaaaaaaa cgctgacggt cttaaagaag gccaaagggt
    28201 atgaagaaac attggatgcc atcaagctgg caatggcgct ttggaagaag aaaatggcaa
    28261 agccaaatcc ggaaactctc gagaaaatgg gcggtggctg ggttggtgaa gaagcgctgg
    28321 cgatttcgct ctactgcgcc ctgtcggcgg aaggcgattt tgccgggggc atccgcctgg
    28381 ccgtcaatca caccggcgat tcggacagca cgggatcgat caccggcaat atcctggggg
    28441 ccatgctggg gaaggcggca attcccgctc actggattga agagctggaa ttgaaagagg
    28501 tgatcgaaga aatggccggt gatctgtttg tcctttttgg caatggcgat cgctggcggc
    28561 agaagtatcc cggattttga agggtaactt tacatgtcga tccatatcat tcccgtcaat
    28621 aaactcagcg cgaaagcctt aaaatgggtt atcgaagaat ttatttccag aaatgggacc
    28681 gattacggcg cagtcgaggc ttcgccggcc accagtttcc agcaggtaaa gtccaaactg
    28741 aaagacggat cggcagttct tgtttttgat gacgagacgg aaaccaccaa tatattcatg
    28801 gctgatgacc ctattttgaa aaagctggat acatagtaac cgggcgatga ggatataaga
    28861 aattatggaa tcattaccat cggcacagaa agagcccgtt tgcattcaca cgttttccgg
    28921 aatcgctttt gatctgctgg acccgaaacc cgagatgatt ctggtcgagg acatcgttca
    28981 ttctctttcc ctcattaacc gtttcaacgg tgctgccaag ttcccttaca gcgttgcgca
    29041 acattcgctc tacgtcgccg gactgctgcc gcctgagctc aaactccaag gtcttttaca
    29101 tgacgcgccg gaggcgtatg tcggcgatat ggtcagccct ctgaagaaaa tcatgaccga
    29161 atacaaggaa gtcgaggcgg ggatcgccag agtggtggcg gaggttttcg gcctggcctg
    29221 tcctgcgcca ccggaggtaa aaaaggccga ccttgccgtt ttgtccgccg agcgggagca
    29281 ggtgctgctg cccagctatg gtccatggta taaaaatttc ccgcttcccg ccgctattcg
    29341 catcgagccg atgtcctgga tgcaggtcaa agagttattt tcatctgaac tcaatgccct
    29401 gctggcaaac cgaccaaagt gatggatcct cacatcgtgt cctcaaaaac ggggggtgac
    29461 atgaacactg ataaatttcc tgtcgtgttg aatgcccttg ctgacagaaa tcaagagatt
    29521 atacagaagt atttgaggga aatatcaaga tctagccgga cctattatat tgataataaa
    29581 gtctataatt gcatttacga aatagcggaa aaagaaggtc ggaaggattt ggcccctcaa
    29641 agcgatgaaa cattatccga gtggcgtgaa agagccggca tcccacacga atatttggag
    29701 ttggaagttt atttgaatca tttgttttca gataagcgga gagacctctt gaagcagccg
    29761 cagcagaagg tatgtgagga agataaggct caatttgcag aactgccgat aactgatgtt
    29821 cctgcggtac aatcaaaaac agatgggcaa ggtcctttag ggcaatctaa aacagaaccg
    29881 gagagatggg gaaatgtcta tctggttgtg tttttattat tcatatttgt aatcggaccc
    29941 gttgtattcg ccttttgcct cgagtccgga atggggggag ccggcttttt catttttctt
    30001 gttccgctgg gcatttttat atggtgccat acacagggga tctggagatc tcccacagaa
    30061 gaggaaaaag aagcaatgag agaagagcag atgagaaaat ggggtacggt taagtcagcg
    30121 cacatcatca gattggaaag gggtctttat acggggct
//
LOCUS       DAOSME010000027        29169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2064_length_29169_cov_25.300027, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000027 DAOSME010000000
VERSION     DAOSME010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 29169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29169
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2064_length_29169_cov_25.300027"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLG98_05700"
     CDS             complement(<1..273)
                     /locus_tag="PLG98_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32783.1"
                     /translation="MNAKGIAYIVTKSNMIAAFGEERWSAFMEKLAGKDSYFSTVILT
                     ITPMPVEKLIICFDEMCAEFFNNDKMQYTYFGKAGAKAVLSPTGPYR"
     gene            complement(666..1496)
                     /locus_tag="PLG98_05705"
     CDS             complement(666..1496)
                     /locus_tag="PLG98_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOE32784.1"
                     /translation="MALFRERDGRRLHVKSRLMGESLVSKKFMEDLKVAPQERLFRDV
                     NIVKIGGQSICDRGVKALPAIVKEVVANKKKHKILLTTGGGTRSRHIYSIGLELGMPT
                     GIIAKFGSSISEQNALLVSTLLAPWGGIKIGHDEITKLYNYFGQDCIPVMHGMPPYDY
                     FALPVAKSRIPIHRTDVGTLVIADLIGARSCIFVKDERGLYTDDPKKNSKAKFIPEIS
                     VQDLLKKNLDDLIIERPCLDILLNSEVIDRIQIINGLEKGNLTKALNGKHVGTIIYKS
                     "
     gene            complement(1518..1745)
                     /locus_tag="PLG98_05710"
     CDS             complement(1518..1745)
                     /locus_tag="PLG98_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32785.1"
                     /translation="MMAGKIFYRERIKVGEKEKKPRFKLVAVADVNITFYADHLKKRE
                     LEEIAASVGAELVLIKAPKDGTVKEEEIEVK"
     gene            complement(1755..2498)
                     /locus_tag="PLG98_05715"
     CDS             complement(1755..2498)
                     /locus_tag="PLG98_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32786.1"
                     /translation="MMAKMRVYIGFDDTDIVEADYGTGKVARRFEKVLPAQCKIWGVV
                     RQQLLVDPAIPYTSHNSSACVVVDCPDSSYVDALKSAAIKHIEAMSLPGSDPGLSVVV
                     EGDLALPALEQFGRQCTARVVTLKDAVKAAGSAHLSGHGGTNGGMIGAAAAVGLTASG
                     WSGRFIEYDGLRDFPDEVIVAALMQKNIRVISLDRDACCPRPEDMVNTKGWLRPRLWG
                     HDVVLPVKPAGAGLWESLGEKRNPDKKKK"
     gene            2694..3146
                     /locus_tag="PLG98_05720"
     CDS             2694..3146
                     /locus_tag="PLG98_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOE32787.1"
                     /translation="MKTDEYKKIIMFAVDNEIEAYEFYKAVADKTKDGGLKKIFTELA
                     QEEAGHRKLLEGLASGKGQMHFDESKDYKVAESINKPKLSMSMKPADAIALAVKNEQE
                     AMEMYKEMAGCSTDAEQKKMFQSLANMEQGHKVKLEDTFTNMAFPEAW"
     gene            3550..3867
                     /locus_tag="PLG98_05725"
     CDS             3550..3867
                     /locus_tag="PLG98_05725"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE32788.1"
                     /translation="MTKSLKFIGSSLDDLRDFPDEARRAAGFELRALQNGLEPRDWKP
                     IVSVGPGVKEIRIHVLGEWRVMYVANIRDAIYVLHAFQKKTQKTGLRDIELARKRYKQ
                     IGE"
     gene            3871..4155
                     /locus_tag="PLG98_05730"
     CDS             3871..4155
                     /locus_tag="PLG98_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE32789.1"
                     /translation="MKKSIVTSSDNVFIDLGYSKDEAAIMQMRADLMADLRRFIKNKK
                     LTQTEAAKIFGVSQSRVSDLITGKWEKFSLEMLIILVTKAGMNVKLKTAA"
     gene            4222..6972
                     /locus_tag="PLG98_05735"
     CDS             4222..6972
                     /locus_tag="PLG98_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32790.1"
                     /translation="MPLSWNEIKTRALAFSKEWQEETSEDAEAKSFWDGFFNIFGISR
                     KRTASFEEPVKKLGEKYGYIDLFWKGVLIVEHKSRGKSLDKAYSQALDYFSGIAERDL
                     PKYVIVSDFARIRLYDLEKHEQHEFDLKDLHKNVRLFGFVAGYQTHKIQEQDPVNIKA
                     AEQMGKLHDRLKESGYTGHSLELYMVRLLFCLFAEDTGIFEKQQFKEYVEQRTSEDGS
                     DLGHHISTLFQVLNTPPDKRQKTLDEQLAAFAYINGKLFEEMLPNAAFDSAMRQELLN
                     CCALDWSRISPAIFGSLFQSIMDKAARRNLGAHYTSEENILKLIKPLFLDELREEFEK
                     IKHNKNRLLEFHKKLRQLNFLDPACGCGNFLVIAYRELRLLELEILRASKIYQSELSI
                     HRLINLNVDQFSGIEIEEFPAQIAQVALWLMDHQMNLLVSEEFGLYYARIPLETSAKI
                     VCGNALTIDWEEVSPARNLSYIMGNPPFVGAKFMDDEQRKDVAAVFGNIKNAGLLDYV
                     AAWYVKAAKMIKGTTICCAFVSTNSITQGEQVGALWNWMLQEGMKIFFAHRTFQWNNE
                     ARGKAAVHCVIIGFADFDITPKWLFEYEHIKGEPQKTKAQNINPYLVDAPDIVLSNRS
                     LPICPVPSIGIGNKPIDGGNYLFTEEEKIEFLKKEPQAEQYFRRWLGADEFLYGYHRY
                     CLWLGDCSPEQLKKMPEAMKRIEAVKNFRLASKSVPTRKIADTPTRFHVENMPSDEYI
                     LISRVSSERRLYLPIGYIEAQNLTSDTALILIGAKLYHFGILTSIMHNAWMRTVCGRL
                     ESRYRYSAGIVYNNFPWPENPTEKQKQAIETAAQGVLDARAQFPNSTLADLYDPLTMP
                     PVLLKAHQTLDRAVDTAYGKTNFTTEAQRVAFLFELYQKYTSLFAADKPKRRAKVVKI
                     PL"
     gene            7026..7484
                     /locus_tag="PLG98_05740"
     CDS             7026..7484
                     /locus_tag="PLG98_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOE32791.1"
                     /translation="MRIVMLALCCFICLLTSAHALEMYKCLDNNGNAVITSTPQDGMT
                     DCVLMDSSEDSVPEDNANDNGSETKESVAGSGNANQQADEKLRRQCANLKVYREEERT
                     YCEGVPQSYKSGNEEMKKKAQDRMASSNASASQLCDYYRGMVRELEAKCP"
     gene            complement(7534..8322)
                     /locus_tag="PLG98_05745"
     CDS             complement(7534..8322)
                     /locus_tag="PLG98_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOE32792.1"
                     /translation="MTDKKNDDIRIAVLENYRKVAVSDQSGCGCSSASCCARPDNAAS
                     LTLGYSANDLSVVPEGADMGLGCGNPQAIASLKAGETVLDLGSGGGFDCFLAARSVGD
                     QGLVIGVDMTPEMIAKARRNAEQGGYANVDFRLGELEHLPVANGIVDVIISNCVINLS
                     PEKEKVFREAFRVLKPGGRLAISDIVATAPIPDEIKKDLALYACCIAGASSVRDIENI
                     MTETGFEQIKIILKEESRIFIRTWLPDSHAEDYVVSATIEAIKP"
     gene            complement(8612..9217)
                     /locus_tag="PLG98_05750"
     CDS             complement(8612..9217)
                     /locus_tag="PLG98_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32793.1"
                     /translation="MEALNCGRNIILGGKFGIGRTRLIREIAKLVNERKFIFVDFSQT
                     PGKMSEKIMKALGIPRRIKNQTQQMGYKSMRYRIAHANTIKQHRPVIVFDNMAKITAQ
                     KKILLRHLILEQHFQFIAVVENFLPQKNLFDLKAQLMPATTLNLRHLKKQEVENLLRT
                     YAKKHHVAWTENDIRNPACLSDGYPLSLKEMLKKKIIPEDR"
     gene            complement(9291..10946)
                     /locus_tag="PLG98_05755"
     CDS             complement(9291..10946)
                     /locus_tag="PLG98_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOE32794.1"
                     /translation="MGNVLPQKSVSDMRRHLKIIADGCSRFRMLSLNRQLEAAQLLLD
                     ENPPIDIAVLGQFKAGKSSFLNSLLGQNVLSVGAIPVTTAVTRLQYGTKAKALVRHFD
                     GNITEIPLADIVKFTSEAENPGNAKNVSIVDIELPSLKKYSGLRLVDTPGLGSVYKYH
                     QSASEEWLPSVGTALLAVSADRPLSEHDLELIRELIAHTPNIILLLTKVDLLSSDQQK
                     EVVAFFQATLNRELHRTLPVYLYSTRDNTDEYKQVIEENIFKTICADRDTEFLRILNH
                     KVRSLSQGCLSYLNIALNSSLQADMNRENLRNRILNEKVNEDIVREELGIISREHQRQ
                     TRALIEAYLEKFRVPLTKKVMRQLVDELPSWKGNLWKLSRKYEVWVSETLSEEMRIIS
                     KNEHRNFLGTMKKAHAAISRSLDAFCNFLGDNIENVLGVKMTEVQWKIDAAEPDHPDI
                     SFTKTFDIHLDLIWFLIPMMFFRKIFERHFIDGIPKEVEINLSRLAYQWEKSVNHAID
                     EMRLQAFNYIHEELATIEALISGTKGQTEDIRGLIDQIEKITI"
     regulatory      complement(10971..11035)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(11638..12345)
                     /locus_tag="PLG98_05760"
     CDS             complement(11638..12345)
                     /locus_tag="PLG98_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE32795.1"
                     /translation="MMVQKDKLSKILNKQNQLPYQIAEYLAEKIIHMQIKPGERILEA
                     KIAQELGVSRSPVREALHILNEKFLVELLPRRGAVVAGFSIKFIEDLYDILKVLYLLL
                     SQKGVSKISDENFNQIAPFFQTMEECANREDFEGYYNAIFDFMEAAIKIVDNPILERL
                     IMNLWPTKKRMMYATLFKRKHDLKSNLDFFLKSVAPYLEKRIDLVENNIVDYLEQEKE
                     NAILLAKEFELSNTTKK"
     gene            12782..13312
                     /locus_tag="PLG98_05765"
     CDS             12782..13312
                     /locus_tag="PLG98_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32796.1"
                     /translation="MRSKRMCKIVPPEKWLKTQITRLCLYFLGMGIETAYKNVPQAKR
                     EMDTLPDQFSFCFNVVNGPSMMILKNGHTVRYVGEKEAYHADLELVLKNIEFAYLMMT
                     GRISTPDAVYHNRQFVRGNLAYMMTMIRVLNIAQTLLFPNFLLRFYIKTVPRLTMKTM
                     VNRAKAYANATIGLFA"
     gene            13345..14550
                     /locus_tag="PLG98_05770"
     CDS             13345..14550
                     /locus_tag="PLG98_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE32797.1"
                     /translation="MQKKEECPKYYEFFNSVKIISGYKALDNIASELDMLGARKPIII
                     TDKGVSQAGLIDHVVNSFGGTQVAIGALYDDTPVDSSIVAVNEIAGIYRQQECDAIIA
                     VGGGSVIDTAKGVNIVISEEADDLMKFTGNNRLTKPMKPFIVVPTTAGTGSEVTMAAV
                     IANPARNVKMQFTSPLILPKVAILDPVMTMSMPPKITAATAMDALTHAVEAYIGPQKN
                     PVSDAFAFSAIELIKANIIDAAKNGKDRDVRLAMANASLLAGISFSNSMVGIVHALAH
                     ATGGLCHVPHGVANAILLPHGLEFYIKKSPDIAARIGKLLRFFMEPEDYAKIPVNERE
                     KNTVAAIRDMNDSLNKICSLPLTLSQAGVTKDKLEAIANAAINDGAIAFCPVEASYED
                     ALEILKAAF"
     gene            14604..16238
                     /locus_tag="PLG98_05775"
     CDS             14604..16238
                     /locus_tag="PLG98_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOE32798.1"
                     /translation="MNAQVSGTAEHQASAAKDQGVKNDVTVCTNPASCEVIAIYPIHT
                     LEDVKNAVTNARRAQPAWQALPLKKKIAYVKKFAEYIQKNSAKLAEIISQDNGKTIFD
                     AMFAEVGPATLATSYYCKNAAEFLADRKLKAGNIMLMNKRSRIVRVPYGVVGIISPWN
                     YPFAIPFSEVVMGLLAGNTVILKAASETQMVGHALKECIDYAGLPEHVFTYINMPGNK
                     AGDALLDAGVDKLFFTGSVPIGKYLMKKASENLTPLVLELGGNDAMIVCEDADLYRAA
                     CGAVWAGMQNAGQSCGGVERIYVDRKIYHEFLAVLKERVEGLRVGDGNCLMTDIGAMT
                     TRKQMRTVQEHIDDALAKGAKIFAQSKCPQNCKGNFIPCTVLTDVDHTMLVMKDETFG
                     PVVGVMPVENMEQAIALANDSNLGLTCSVWSKNRKKAVQIGRRVMAGAVMINDHLMSH
                     GLAETPWGGFKESGIGRTHGDIGFAEMTQPQVIVDDILSAMPFVRRNFWWQPFEKLQY
                     DGMKGLIDLFHGKGFWERFVGLNKLGHAFYRSFVKK"
     gene            16481..19183
                     /gene="fdhF"
                     /locus_tag="PLG98_05780"
     CDS             16481..19183
                     /gene="fdhF"
                     /locus_tag="PLG98_05780"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:17543..17545,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOE32799.1"
                     /translation="MSETFLLNGKETPFEQGQTIVEAAKNGGVEIPTMCYLKGSTPTG
                     ACRICCVEVKGARSLVAACSTPVTPNMEVFTETELVHKSRKMVVEFMLTSGKHNCMTC
                     EANGNCRLQDLAYFYKITTPRFEESEQLYPTEDENPFIVRDFSRCILCGRCVQACNEV
                     VVNRAISQGYRGRKSKIVTGGDLPYNHDDSECVFCGQCVQVCPVGALTEKKAKGLGRP
                     WEVQKIRTTCPYCGVGCQQLLHVKDGKIVKVTAVEDAQPNQGRLCVKGRFAYDFIYSG
                     ERLKTPLIREKNGEFREASWDEALDLVANKFKEIIKKHGPDAVAGVSCARSINEDSYQ
                     MQKLFRAVFGTNNIDHCARTUHAPTVAGLATSFGSGAMTNSFADYAKAKMIILVGTNI
                     SEAHPVASTFVKDAVLAGAKLIVIDPRRTDMVDFADVHIPLKVGSDVALINALMNVLI
                     TENLYDKKYVESCTEGFEELKKKVMEYTPEKAAKISGIPADTIRELARTMASIKPAML
                     MYTLGITEHICGVNNVMSCANLQMLLGNVGVELGGVNPQRGQNNVQGACDMGALPNVF
                     PGYQQVANAEARAKFEKAWNVKSLPDKPGMMMPTMMKNLTTGKVKGFYVFGENLANTE
                     PDIKHVEQELQSAEFLVCQDIFPTETTRFAHVILPAAAWSENDGTFASSERRVNRVRT
                     ASVPPGEAKPNWWIFKEIAKRFGHEWASNSAQEIWDNEVSVLAPSMAGIKYARLEGDG
                     LQWPCPTADHPGTPTLHKGGKFTRGLGMLKAIDYIPPAEVPDKEYPMVLSTGRRLYHY
                     HTRTQTGRSEGINDLLGEETADISPADAEAKGIKNGEKILVKSRRGKVEVKARVTEEV
                     PQGLVWMAFHFREACANWLTNPVYDPVTQTAEYKACAVKIEKLK"
     gene            19381..20154
                     /gene="modA"
                     /locus_tag="PLG98_05785"
     CDS             19381..20154
                     /gene="modA"
                     /locus_tag="PLG98_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE32800.1"
                     /translation="MKKSNFKQVITKILLTVIGVAFFAGLSGQAFAEEKISVAVAANF
                     ISAFKDMGADFEAKTKIKVEGTFSSSGNLYSQITNGAPYDLLLSADEERPAKLNKDGL
                     ADNPFIYARGQAILWSANKEFCKAKTWQDALKNEQIKKLAIANTQTAPYGTAAQKALE
                     KAGMWTDVQSKLVVAQSIAQSFQYASTESVDAGFCALSATASPEGKKGCFYVLNEAPE
                     IIQSACILKRTTNRAAAEKFAEYLRSPAAQEIKVKYGYR"
     gene            20155..20814
                     /gene="modB"
                     /locus_tag="PLG98_05790"
     CDS             20155..20814
                     /gene="modB"
                     /locus_tag="PLG98_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOE32801.1"
                     /translation="MDYLAIYVTLKLALVTTVVLMVIAAPVAYALAYNRFSGKSFLEA
                     LIYLPMALPPTVIGFYLIILMGPKGFVGKMWQMLTGGSLLFTFIGITMASIIYSIPFA
                     VQPMKAAFSKIDRRLLESAYVLGLSKKATFFRVIIPNSVSGIAAAAILVFLHSIGAFG
                     VLLMVGGSIPGETKVASIAIYEAVEMMNYKAAGLIALSFIPISYAFLLLVNKLNEGAR
                     S"
     gene            20811..21524
                     /locus_tag="PLG98_05795"
     CDS             20811..21524
                     /locus_tag="PLG98_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE32802.1"
                     /translation="MSIEVSFKKKLKYFDVDISFSCAKKNMVVMIGPSGGGKTTIIRM
                     LAGLEKPKEGKVVFGDEVWFDSLRRIDVPPQKRRLGYVFQDYNLFPHLNLFDNAAFAA
                     VDKKEVEDLFGIFRIGHLKKRKPHMVSGGERQRCAICQALARHPRILLLDEPFSALDA
                     ITRRGLREELKNLKGKMSFPIIYVTHDITEALYLADELLPVVDGKIDDQWLQRMMTRE
                     PSSEVALRAAREPRLTLVY"
     gene            21731..22312
                     /locus_tag="PLG98_05800"
     CDS             21731..22312
                     /locus_tag="PLG98_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOE32803.1"
                     /translation="MNICTYSYEEYIHLVKSFHGTLAPGLLIGGFIVDLAMKNLPEGE
                     FFDAVCETPVCLPDAVQILTPCTIGNGWLSIVNFGRFAVTLYEKYSGRGVRVYLDTEK
                     LNNWPEVRDWYLKKKKKDQQDAEVLFAQIKEAGHQLLSVQKVQVEPEKVRRKKMGPVG
                     ICPACGEAYPLKDGGKCMNCQGATPYSSVVPVK"
     gene            22335..23108
                     /locus_tag="PLG98_05805"
     CDS             22335..23108
                     /locus_tag="PLG98_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOE32804.1"
                     /translation="MLKTGITLSRLFEKTEPAARNRQIADLRIGLSYVGIKLDNGVAG
                     LAAVLPGSTARGCTVLKEAGTYIGSPAVETLQYLVEAKDALRRAIGVATANALVLPES
                     GKTEDREATTYLNLQPGEKVAMVGLFAPLVERIRSTGATLTVVEKNPERLEVLSAEEK
                     QKALKDCDVAIITATTLLNGTFEETIGLLGRPRSVVLMGPSTPLVPEIFHDTPVTQLG
                     GSIVADAAKALQVISEGGGTPALRHYLRFVNLLLPDKSR"
     gene            complement(23113..23904)
                     /locus_tag="PLG98_05810"
     CDS             complement(23113..23904)
                     /locus_tag="PLG98_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE32805.1"
                     /translation="MNLFDKDIQISESDHSRFAGTISENWSVNGNPNGGYLMAMIVNA
                     MLRKSDKKATPVVTANYLSRCVPGKIEIHIAEITRSRQFTRFEARLIQEGDEKIRALG
                     TFVSDNNDCAITRYESKAPELAPIEECVPMSVPPSYTLFQNLDLRLDPACAGWMTGGE
                     LTDVSLNKGYFRFRDGRPLDLFSLFLIIDSMPPAAMASQGATAWVPTIELSVNVRNLP
                     KTDRLKCSLRTRFITCGILEADGEVWDEKGNLAAISRQIAQFKKI"
     gene            complement(23985..24995)
                     /locus_tag="PLG98_05815"
     CDS             complement(23985..24995)
                     /locus_tag="PLG98_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE32806.1"
                     /translation="MPDTLKQYAEKLGFPGSETMAKIFAILYDNADSVAVAEALPGSV
                     ADIAARTGLNEERVRQIAQKLLRKGAISRVLAKGDYYRLYPAMIELRDAVVIMPDCPE
                     ELFELWEIIIKEEMPRIVPVFNKLNLTPMLRVIPIEETVENSSRVLDADSARSIIRSA
                     RIITAIPCPCRTQAKHLGKTKDCIAPDGVNLCMQINGFAEAAIDRGVGEVLSTDEALR
                     RLAVAEEAGLVHTVRNNVKEDMFMCNCCPCCCTAMFLHNQAGFDASFAPSRFLAQVDA
                     DTCTGCGACEEKCAFNAITVGDTAVIDCNKCMGCGNCVIVCPVEAIALQEARPPEFIR
                     RT"
     gene            complement(25101..27335)
                     /locus_tag="PLG98_05820"
     CDS             complement(25101..27335)
                     /locus_tag="PLG98_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE32807.1"
                     /translation="MGALHKTGCVLCAQNCGLEVDVDNNRIVKVKPDKSNPKSEGYFC
                     RKGMNVAFHQHNADRLKYPLKKVGNHFERITWDQAIDEIAAKLKDIVGQYGPRSFAYM
                     GGGNQSCHLEVAFGVSLMRTLGSQYQYSALAQEFSGAFWVCGRTHGKQYLHDGPDVKN
                     TDVLLIFGWNGMESHQIPQAPRQLQRLSKDPDKLLIVVDPRISETAKIADIHLRIRPG
                     TDALLLKSMIAIILKEGWINKDYMENHTAGFEQVKSLFENFDVRAALKVCELDFDQVY
                     KVTQAYAKRKSSLRYDLGIFMGRHSGLNSYLIVMLQSICGRLCVPGGNIIPGHIVPFG
                     PNSDERNQKTWRTVATNAFPVCGCFPPNVMPEEILSDHPERLRAVLVSGLNPLRSYAD
                     TTAYEKAFQRLDLLVTVELAMTETAALSHYVLPSRSGYESWDGTFFPFTYPEIFFQMR
                     RPIIAPEGEPLEQGEIDLRLADKLGLIPPIPEKLYKTAEESHAAFRQALMEYAMTEPK
                     AMKTMPFILGKTLGKAMGSVHLAALWGMLQVAPQSFQASAARAGFTPGPTLGEEIFRK
                     ILDHPEGLWVGKTDEGNNFAEIKTPDGKINLFIPELLDELKSIDAQREEAALVMPAEF
                     PLVLMAGRHTSMNANTLMRNPAWNEGKRPCTLAMHPDDAAVLNLKDGQPVRVTTEAGS
                     EEIELEITETVRPGHVTMPHGFGLIYNGVKYGANVNRLTKTTNRDQFGTPMHRYVRCR
                     VEAA"
     gene            complement(27386..27808)
                     /locus_tag="PLG98_05825"
     CDS             complement(27386..27808)
                     /locus_tag="PLG98_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOE32808.1"
                     /translation="MQEKDCIFFQLAKANQLASRFLSQKVSELNLTPVQALVLGFLHQ
                     EDQITSAELGKKTGLDSATLTGILDRLEAAEFIERKNNPEDRRSIHIHLTLKGNALSK
                     EAIRVIAGANLEFLQSLTKEEKRHLFDIIKKLRLGAAR"
     gene            28332..>29169
                     /locus_tag="PLG98_05830"
     CDS             28332..>29169
                     /locus_tag="PLG98_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:28887..28889,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE32809.1"
                     /translation="MNVSRRGFLKISGAVAAVSGLGISLKSIAAHARELKIMHAKETT
                     TVCPYCSVGCSIIVSVRDGKVINTEGDPDSPINRGALCSKGGSIYQMAVNENRLGKPL
                     YRAPYAREWKEVEWEWAIDKIAQNVKKSRDASFKKVNAKGEVVNRTEGIASVGSAAMD
                     LEECFTYQKFLRGLGLVYIEHQARIUHSPTVPALAESFGRGAMTNHWVDFKNADVILI
                     MGSNPASNHPVSWKWIQEAVDKRGAKIICVDPRFTQSAAKAHLYAPLRSGTDIAFLGG
                     MIK"
BASE COUNT         7506 a         6907 c         7308 g         7448 t
ORIGIN      
        1 cctgtagggt cccgtcggcg acagaaccgc tttggcgccc gccttgccga aatatgtata
       61 ctgcattttg tcgttgttga aaaattcggc acacatttca tcaaaacaaa tgattaattt
      121 ttccaccggc atgggagtaa tggtgaggat gaccgtgctg aagtaactgt ccttcccggc
      181 gagtttttcc ataaaggccg accatctttc ctcgccaaat gctgcaatca tatttgattt
      241 tgttacaatg taagcaatgc ctttagcgtt catgtctgaa atcctccccc ggattgttct
      301 gtcatcaagg tgtttccaac gtctgttatg tgcggcatgg tgcaatctaa cataaaatgg
      361 ccggatagaa aattatgaat ctttaaaatg agcgatcgga agcgattcgc atcactgccg
      421 gatgtccggc aaaacgtatc acggcgatca atctacattg ctccggaaaa ttaatcaatc
      481 cctctaaaga gggatatgtg tcatttttac ataattgctg ttccagtaat ggccgacttt
      541 actgtttgtc tcatcggcag caacaaaaag attttatggc ggataatcag ggggaaatct
      601 gattgcaaaa aaagaaggct tcgtaatacc gaagccttct tttttgtgat gatgcggcct
      661 gacttctatg acttgtaaat gattgtgccg acgtgtttgc cgttaagggc cttggtcagg
      721 ttgccctttt ccagaccatt gataatctga atcctgtcga tgacttcact gttgagcagg
      781 atgtccaggc acggtctttc gataatcaga tcatcaagat tttttttcag aagatcctgg
      841 acgctgattt cgggaataaa tttcgccttg ctgtttttct tcggatcgtc cgtgtagagt
      901 ccccgctcgt ccttgacaaa aatgcaactg cgggcgccga ttaaatcggc aatcaccagc
      961 gtgcccacat cggtgcggtg aatgggaatg cgggattttg ccacgggcag ggcaaagtaa
     1021 tcgtaaggcg gcatgccgtg catgacggga atgcagtcct ggccgaaata attgtatagt
     1081 tttgtaattt catcatggcc gatcttgatg ccgccccagg gagccagcag tgtggaaacc
     1141 agcagggcgt tctgttcgga aatggaactg ccgaacttgg cgatgatgcc ggtgggcatc
     1201 cccaactcca aaccgatgga ataaatgtgg cggctgcgcg tgccgccgcc tgtcgtcagc
     1261 agaatcttgt gtttcttctt gttggcgaca acttccttga cgatggccgg caaggccttc
     1321 accccgcgat cgcaaatgga ttgcccgccg atcttgacga tattcacatc ccggaaaagc
     1381 ctttcctgag gagccacctt caggtcttcc ataaattttt tgctcaccag gctctcgccc
     1441 ataagcctgc ttttcacatg cagcctgcgt ccgtctcttt ctctgaacag tgccataaat
     1501 tcaatcctca agaatgttta cttgacttca atttcttctt ccttgactgt tccatctttc
     1561 ggcgccttga ttaaaacaag ttccgcgccc accgaagcgg caatttcctc gagctctctt
     1621 ttcttcaggt ggtcggcgta aaaagtgata ttcacatcgg caaccgccac cagtttgaaa
     1681 cgcggcttct tctctttttc gccaaccttg attctttcgc gataaaatat tttccctgcc
     1741 atcattctac ctccctattt tttctttttg tcagggtttc gtttctctcc caggctttcc
     1801 cacaggcccg cgcctgccgg tttaaccgga agcacaacgt catgccccca gagcctgggt
     1861 cttaaccagc cttttgtatt caccatgtct tccggacggg ggcaacacgc atcccggtcc
     1921 agagaaatca cccgaatatt tttttgcatc agcgccgcaa caattacctc gtcgggaaaa
     1981 tctctcaacc catcatactc gataaaacgt ccgctccatc ccgaagcggt gagaccgaca
     2041 gccgccgcgg ctccgatcat gccgccgttg gttccgccgt gaccggaaag atgagcgctg
     2101 cccgccgcct ttacggcgtc cttcagggta acgactcttg ctgtgcactg ccttccaaat
     2161 tgctccagcg caggcagcgc cagatcgcct tcgacaacca cggaaaggcc gggatcgctg
     2221 ccgggaagcg acatcgcttc gatgtgttta atggccgcgc tttttaaagc atccacatac
     2281 gatgaatccg ggcagtccac aaccacacag gctgaactgt tgtgcgacgt ataaggaatg
     2341 gccggatcta caagcaactg ctggcggaca acaccccaga ttttgcattg agcaggcagg
     2401 actttttcaa aacggcgggc gacctttccc gttccgtaat ccgcctccac aatgtccgta
     2461 tcgtcaaaac caatataaac gcgcatcttt gccatcatgt taaaaaaaac atccttaacc
     2521 gattatagag tcaccgtttt agcctgtcaa tgaattaaaa atgtaaacat cgaaattctc
     2581 aaatgatttg tgataatata tactttgacg caatccatta tttcctgtat attactaaaa
     2641 cgattcgaaa tgaattaaat gaagatcata gatgattgcc aaggaggatt gaaatgaaaa
     2701 cggatgaata caaaaaaata atcatgtttg cagttgacaa tgaaattgaa gcctacgagt
     2761 tttacaaggc ggttgccgat aaaacaaaag atggcggttt gaagaaaatc tttacggaac
     2821 tggcgcagga agaagccggt catcgcaaac ttctggaagg cttggcatcc ggaaagggcc
     2881 agatgcattt tgacgaatcc aaagattaca aagtggccga atcaatcaat aagcccaaac
     2941 tgagtatgag catgaaaccg gcggatgcca tcgcgctggc ggtcaagaat gaacaggagg
     3001 ccatggaaat gtacaaggaa atggccggat gcagcaccga tgccgagcag aaaaagatgt
     3061 tccagtcgct ggcgaatatg gaacaggggc acaaagtcaa actggaagac acctttacca
     3121 acatggcgtt tccggaagcc tggtaaattt gattgaataa atatttaagg cgggggaaaa
     3181 cgtttcatta tcccccgcct ttttctttat ggtcattcct tggattgtaa attaaaaacc
     3241 cacctgatgt taattgttga aaattgctta ttaataaaaa tattatagtt tacagaatct
     3301 tgcaacatta cctgttaaat gttggcggct actttcaagt actattttat aaatgaaaca
     3361 tttattttga tcattctaga acgttttgtg aaaggcggaa ggttaaacaa gcaacaagca
     3421 taaaaatatt cttgaaatgt aatcaataag ttgtattatc tccagcaatt aatcaaataa
     3481 atatttctta agcagacggg caattattta tattgctaaa tatataaaaa cttatataat
     3541 gggattgcga tgacgaaatc gttaaaattt atcggttcaa gtctggatga tcttcgtgat
     3601 tttccggatg aagcgcgtcg agcagcaggt tttgaacttc gcgcacttca aaatggactg
     3661 gaaccacgcg attggaaacc cattgtgtct gttggaccgg gcgtaaaaga gatccgcatt
     3721 catgttcttg gtgaatggcg ggtgatgtac gttgccaata tccgggatgc catttacgtg
     3781 ttgcatgcgt ttcaaaagaa aacgcaaaaa accggtctgc gtgatattga attagcgcgt
     3841 aaaagataca agcaaattgg agagtgattt atgaaaaaat ccattgtaac ttcatcggac
     3901 aacgtattca ttgatttggg atattccaaa gatgaagctg cgattatgca aatgcgggcc
     3961 gatttgatgg ccgatttgcg cagatttatc aagaacaaaa aacttactca gactgaagct
     4021 gccaaaattt tcggcgtcag ccagtcgcgg gtttccgatt tgattaccgg caaatgggaa
     4081 aagttcagtc ttgaaatgct gatcatcctt gtaaccaagg ccggaatgaa tgttaaactg
     4141 aaaacggccg cgtagtcgcg cagtcatgat tcaagattcg caaatacttc tcacgctgat
     4201 gttaaaaaaa gaaaggattc gatgccgcta agctggaacg aaattaagac acgcgcgctc
     4261 gctttttcca aagaatggca ggaagaaacc tccgaggacg ccgaggccaa atccttctgg
     4321 gacggctttt tcaacatctt cggcatttcc cgcaaacgaa cagccagttt tgaagaaccg
     4381 gtcaaaaagc tgggcgaaaa gtacggctac atcgatttat tctggaaggg cgtgttaatc
     4441 gtcgaacaca aatcgcgcgg caagagtctc gacaaggcct actcacaggc gctggattat
     4501 ttctccggca ttgccgagcg cgatttgccc aagtatgtca tcgtttccga cttcgcccgc
     4561 atccgccttt acgatctgga aaaacacgaa cagcacgaat tcgacctgaa agacctgcac
     4621 aagaacgtgc gcctcttcgg tttcgttgcc ggataccaga cccacaaaat tcaggagcag
     4681 gaccccgtca acatcaaagc cgccgagcag atgggcaaac tgcacgaccg gctgaaggaa
     4741 tccggctaca caggccattc gctcgaactc tacatggtgc ggctgctgtt ctgcctgttt
     4801 gccgaagaca cgggtatttt tgaaaagcag caattcaagg aatatgttga acagagaacc
     4861 tctgaagacg gctccgacct tggccaccat ataagcacct tgtttcaggt gctgaacacg
     4921 ccaccggaca agcggcagaa aacgcttgac gagcagcttg ccgcttttgc gtatatcaat
     4981 ggcaaactct ttgaagaaat gctgcccaat gccgccttcg attccgccat gcggcaggaa
     5041 ctgctcaact gctgcgcgct ggactggagc cggatttcac ccgccatttt cggttcactc
     5101 ttccagtcca ttatggacaa ggccgcgcgg cgcaacctcg gcgcgcatta caccagcgag
     5161 gaaaacattc tcaaactcat caaaccgctt tttctggatg aactccggga agagtttgaa
     5221 aaaatcaaac acaacaaaaa ccggctgctg gaatttcaca agaaattaag gcaactgaat
     5281 tttctggacc ccgcctgcgg ctgcggcaac tttctggtga ttgcctaccg cgaactccgc
     5341 ttactggaac tggaaattct gcgcgcatca aaaatttatc aaagcgagct gagcattcac
     5401 cggctgatta atctcaatgt tgaccagttt tccggcatcg agattgagga atttcccgcg
     5461 cagattgcgc aggtggcgct gtggctgatg gatcaccaga tgaacctgct ggtttccgaa
     5521 gagttcggcc tctactatgc gcgcattccg ctggagacca gcgccaagat tgtctgcggc
     5581 aacgcgctga ccatcgactg ggaagaagtt tctcccgccc ggaatctcag ttacatcatg
     5641 ggcaaccctc cgtttgtggg agcgaagttc atggacgatg agcagcgcaa ggatgttgcc
     5701 gctgttttcg gcaatataaa aaatgccggt cttctggatt atgtggccgc ctggtatgtg
     5761 aaggccgcga agatgatcaa aggaacaacg atttgctgtg cctttgtttc caccaattcc
     5821 attacgcagg gcgaacaggt cggcgctctt tggaactgga tgctgcaaga aggcatgaaa
     5881 atattttttg cgcaccggac gtttcagtgg aacaacgaag cgcgcggcaa ggcggcggtg
     5941 cattgcgtga ttatcggctt tgccgatttc gatattaccc ctaaatggtt atttgaatat
     6001 gaacatatca aaggcgagcc gcagaaaact aaagcgcaaa acataaatcc atatctggtt
     6061 gatgcgccgg atatcgtttt aagcaatcga agtctgccga tttgtccggt tccatctatc
     6121 ggcataggca ataaaccgat tgacggcggc aactatctgt ttacagaaga agagaaaata
     6181 gaatttctaa aaaaagaacc acaggcagag cagtatttcc gccgctggct gggagcggat
     6241 gaatttttgt atggttatca tcgttattgt ctttggctgg gtgattgttc gccggagcaa
     6301 ttaaagaaaa tgccggaagc gatgaaaaga atagaagctg tgaagaattt tcgattagcc
     6361 agtaaaagcg ttccgaccag aaagattgct gatacaccaa cccggttcca tgtcgagaat
     6421 atgccaagtg atgaatacat tttaatttcc agggtttctt ctgaacgtag actttaccta
     6481 ccgattggtt atattgaagc gcaaaatctg acaagtgata ccgcgcttat tcttatcggt
     6541 gcaaagcttt accattttgg tatattaaca tctatcatgc acaatgcatg gatgcgcaca
     6601 gtatgcggcc gtttggaaag ccgctatcgt tattccgcag gaattgttta caacaatttt
     6661 ccctggccgg aaaatccgac ggagaaacaa aagcaggcca ttgaaaccgc cgcgcagggc
     6721 gtgctggatg cccgcgcgca atttccgaac tcgacgttgg ccgacctcta cgatccgctg
     6781 accatgccgc cggtgctgct caaagcccat cagacgttag accgcgccgt ggacaccgcc
     6841 tacggcaaaa ctaattttac aaccgaagcc cagcgtgtgg cgtttctctt tgaactttat
     6901 caaaaataca ccagcctctt tgcagcggac aaacccaaac gccgagcgaa ggttgtgaaa
     6961 ataccattgt gatttatacg gcgtgttcag ggaagcctga tgtttagagt gaaggagaaa
     7021 ataaaatgag aatcgttatg ttagcgttgt gttgttttat ttgtttgttg acgtccgccc
     7081 atgcgctgga gatgtacaag tgcctggata ataacggcaa cgcagttatt accagcacgc
     7141 ctcaggatgg gatgacggat tgtgtgttga tggattcttc agaagattca gtgcctgaag
     7201 ataacgcaaa cgataatgga agtgaaacaa aagaatcggt cgcgggctca ggcaacgcca
     7261 atcagcaggc ggacgaaaaa ttgcgtcggc aatgcgccaa tttgaaagtt tatcgcgagg
     7321 aagaaaggac ttattgcgaa ggtgtgccgc agagttataa aagcggcaat gaggagatga
     7381 agaaaaaggc acaagaccgg atggcgtctt ccaacgcgag cgccagccaa ctctgcgact
     7441 attacagagg tatggtcaga gaattggaag caaaatgccc ctgataatat gacctgcgat
     7501 caatccgcaa acatttgaac ggattttgtc ccgttacggt ttgatggcct cgatggttgc
     7561 ggaaacaaca tagtcttcgg cgtggctgtc aggcagccag gtgcgaataa atattctgct
     7621 ttcttccttg agaattattt ttatttgttc aaaacccgtt tcggtcatga tgttttcaat
     7681 gtcccgcacg gaagaggctc ccgctataca gcaggcataa agggccagat cttttttgat
     7741 ttcatcgggt atcggggccg tggcgacaat atcggaaatc gctaaccggc cgcccggctt
     7801 taatacgcga aacgcttccc ggaatacttt ctctttttcc ggcgacagat tgatgacgca
     7861 atttgaaata ataacatcca cgatcccgtt cgctaccggc agatgctcca gttctcccag
     7921 ccggaaatca acattggcgt aaccgccttg ttcagcattg cggcgcgctt ttgcaatcat
     7981 ttccggcgtc atatccacac cgatcacgag accttgatcg ccgacagacc gtgccgccag
     8041 aaaacagtca aagccgccgc cgcttcccag atccagcacc gtttctccgg ctttcaacga
     8101 ggcaatggcc tgaggatttc cgcagcccag ccccatgtcc gccccttcgg gaacaacgga
     8161 taaatcgttc gccgaataac ccagcgtaag agacgcggcg ttatccggtc ttgcgcaaca
     8221 ggaggcgctg gaacagccgc aaccggattg atcggacacg gcgacttttc gataattttc
     8281 caaaaccgca atgcggatgt cgtcgttttt tttatcggtc atttttagcc tctgctttca
     8341 tcgaagcaat atattgaaga ttacagatta ttttctatca caaaattgcc gtttcaggaa
     8401 tgaagaaatc tggaggagag gatttcctgg acaaacccaa acgctgccgg tttacttttt
     8461 taaatcttaa gcagtctgac ggcttcttcc atttcggcgg cttctttcat ttttgcccgc
     8521 atctgccggc gattcctgaa ggttttccaa atgaaccgtt gcataacata attttaagca
     8581 tgttctcccg gtaatgtttt ttttaaatct ttcatctgtc ttctgggatt atcttttttt
     8641 tcagcatctc ttttaaactc agtggatagc catccgataa gcaagctgga ttgcgtatat
     8701 cattttccgt ccacgcaacg tgatgttttt ttgcatacgt gcgcaacaga ttttcaacct
     8761 cctgtttttt aagatggcgc aggttcaaag ttgttgcagg catgagttgc gccttgagat
     8821 caaacaaatt tttttgcggc agaaagtttt ctacaacggc aatgaattga aaatgctgct
     8881 ccagaatcag atgacgaagc aatatttttt tctgagccgt tattttagcc atgttgtcga
     8941 aaacaataac cggtctgtgc tgtttgattg tatttgcatg agcaatgcga taacgcatgg
     9001 atttatagcc catttgttgt gtctgatttt taattcttcg ggggatgccc agcgctttca
     9061 taattttctc gctcattttt cccggcgttt gactgaaatc gacaaagata aattttcttt
     9121 cattgaccag cttcgctatt tctctgatta aacgggttcg tcctatcccg aactttccgc
     9181 ccagaataat atttctgccg caattcagcg cttccataat tattttcttc tcgctttggc
     9241 gtccgacaaa taacataatg actctgtaaa acttcgggtg agtatggcga ttatatggtt
     9301 attttttcga tctgatcaat aaggcctctg atgtcttcag tttgtccctt tgtcccggaa
     9361 atcaacgctt cgatcgtggc gagttcttcg tgaatatagt tgaaggcctg caggcgcatt
     9421 tcatcgatgg catgattgac gctcttttcc cattgatagg caagccgcga cagattgatt
     9481 tccacttctt tcggtatgcc gtcgataaag tgccgttcga aaatcttgcg gaaaaacatc
     9541 atgggaataa gaaaccatat caggtccaaa tgaatatcaa aggttttggt aaaagagata
     9601 tccggatggt cgggttcagc ggcatcgatt ttccattgaa cttccgtcat cttcacaccc
     9661 agcacgtttt caatgttatc tcccagaaaa ttgcagaaag cgtccagcga acgggatatg
     9721 gcggcgtgcg cttttttcat ggttcccaga aaattgcgat gttcgttttt ggaaattatc
     9781 cgcatttctt cggagagcgt ctccgacacc cagacctcgt attttcggga tagtttccag
     9841 agattgcctt tccatgaagg caattcatcg accagttgac gcataacttt ttttgtaagc
     9901 ggcactctaa atttttccag ataggcttca atcagagcgc gcgtttggcg ctggtgttcg
     9961 cgggaaataa tacccagttc ctcccgcacg atatcttcat tgactttttc attcagaatc
    10021 cggttgcgca gattttcccg gttcatatcc gcctgcaacg acgaattcag cgcaatattg
    10081 agatagctca ggcagccttg agagagcgac cggactttat gattcaggat tcttaaaaac
    10141 tccgtgtcgc ggtcggcgca gattgtttta aaaatgtttt cttcaatcac ctgtttgtat
    10201 tcgtccgtgt tgtcccgcgt cgaataaaga taaaccggca gtgtccggtg aagttcccga
    10261 ttaagtgttg cctggaaaaa ggctaccact tctttttgct gatccgatga aagcagatcg
    10321 actttggtca gcaataaaat aatattcggc gtgtgcgcga ttaattcacg aatgagttcc
    10381 aaatcgtgtt ccgataaggg tcggtcggca cttaccgcca gaagcgctgt gccgacggag
    10441 ggcagccatt cttccgaagc ggactgatga tatttataga cactgcccag tcccggcgtg
    10501 tccaccaggc gcagaccgga atatttcttc agggacggca gttcaatgtc gacaatggat
    10561 acatttttcg cgttgcccgg attttccgct tcggaggtga attttacaat gtccgctaac
    10621 ggaatttcgg tgatgtttcc gtcaaagtgc cgtacgaggg cttttgcttt cgtcccatat
    10681 tggagacgtg taacggccgt ggtcaccgga atggcgccga cggacaaaac gttttgtccc
    10741 agaagactat ttaagaaaga acttttcccg gccttgaatt gtcccagcac cgcaatgtcg
    10801 atgggcggat tttcatcaag cagtaattgc gcggcttcca actggcggtt cagggaaagc
    10861 atccggaaac gcgagcaccc atccgcaatg attttcagat gacggcgcat gtcggacacg
    10921 gacttttgag gtaagacatt tcccataata aaaaaactcc cgcagaggta ttttctgcag
    10981 gagttatcag caataattgc ggttatctca ggtgaactcc atcacccgtt tgcctgtttg
    11041 cgtggaccat attcaaaaaa gagtatttag tcaatatgtt tgtcgagcaa tttattaacg
    11101 gtctcacaat catactgtta ttaagcgatt aacaaattct cggatgtctt gccggcgaag
    11161 gccgggaacc agtaaaaaat aatgccggat gccggccgtc gccggcatga cgaatgtaaa
    11221 tttgaatatt cgacatttaa taatttgatt tgatcatgtg tgagtccgct agaagtcatt
    11281 cggaatgttt ttccagtgat cgcgtatcac acgacaaaca tcgggcggca gaattttatt
    11341 ttgtgtttgt tgttgcaagc cgcgcaggct atctccagat gaatttgcgt acacgtcact
    11401 ccctgctcga tccatgtttt atttccatga gggcgccgca tgaatgcggc tgcataagtc
    11461 attgtgaatg gatgttaatt gaggacggaa aacttgagct acgcgatttt gaaatatccg
    11521 cttcttgatc catcagtaga ttcatcctat atgaaaaata atttacgaag tttgtgcacg
    11581 gattataata tgagattata taaaatgatt ttgaataaga aatgaaaacc ggaatgttta
    11641 tttttttgtt gtgttactga gttcaaactc ttttgccaac aggatggcat tttccttttc
    11701 ctgttcaaga taatccacga tgttgttttc aaccagatca atccgctttt ccagataggg
    11761 cgcaacgctt ttgagaaaga aatccagatt tgatttcaaa tcgtgctttc gtttgaacaa
    11821 agtcgcatac atcatccgct ttttggtcgg ccagagattc atgatcaagc gttccagaat
    11881 aggattgtcg acaattttaa tcgccgcttc catgaaatca aaaatggcat tgtagtaacc
    11941 ttcgaagtct tctcgattgg cacattcttc catggtttgg aaaaatggcg ctatctgatt
    12001 aaaattttca tccgaaattt tcgacacgcc cttctgagaa agaagcaaat aaagcacttt
    12061 gagaatgtcg tataaatctt caatgaattt tatggaaaag cccgctacga cagcgccgcg
    12121 gcgcggcagt aattccacca gaaatttttc attgagaatg tgtagtgctt cccggacggg
    12181 gctgcggctg acaccaagtt cctgggcgat ctttgcctcc aaaatgcgtt cccccggttt
    12241 gatttgcatg tgaatgatct tttcggcaag atattctgcg atttgatagg gtaattgatt
    12301 ttgtttgttt aatatttttg ataacttatc tttttgcacc atcatggata gccttgagtc
    12361 gtttccagaa taagctgtta gtttgtatat attattatca ttatcacaaa aaatttattt
    12421 ccgatatgaa taaatcacga atatcattat gttaataatg atataagaga acccatcttc
    12481 aaataaaata tcataaaata aacaataaat tcttaatgat atgaaaacaa aagggttgac
    12541 aagaccatga ttctgctata tgtcgattgt cgacaatcga caatgtgcgt gtaagtttcc
    12601 attccctata aaagatatta aacattaaaa tcttcaattt cgtaaggaaa tgaggggccc
    12661 tgaatgtttt tgatatgttt ttcatatcgc ttctctaatt ttcatttccc gagattgctc
    12721 aatcattttt tccattcatt aaactcacaa aacattaaag aaataaaaaa ggggggacgt
    12781 tatgaggagt aaaagaatgt gtaagattgt gccgcctgaa aaatggttaa agacacagat
    12841 taccaggttg tgtctttatt ttttaggaat gggaattgag acggcatata aaaatgtgcc
    12901 gcaagcaaaa agggaaatgg atacgttgcc ggatcaattt tcattttgct ttaacgtcgt
    12961 caacggtccg tcgatgatga ttttgaaaaa cgggcacacg gtcaggtatg ttggcgaaaa
    13021 agaagcttat catgccgatc tggaactggt gctcaagaat atcgaatttg cctatctgat
    13081 gatgaccggc agaatcagca cgcccgacgc ggtttatcat aaccgtcagt ttgtcagagg
    13141 aaatctggct tatatgatga cgatgatccg ggttctgaac atagcccaga cgctgttgtt
    13201 tccgaatttt ctcctgcggt tttacattaa aacagtcccg cggttaacta tgaagacgat
    13261 ggtcaaccgc gccaaagcat atgccaatgc aacaatagga ttattcgctt aaaagacaat
    13321 cacttgagga gggatattgt gaatatgcaa aagaaagaag aatgcccgaa atactacgaa
    13381 ttttttaatt ccgtaaaaat aatttcaggg tacaaagccc tggacaacat cgcctcggag
    13441 ctcgatatgc tcggggccag aaagcccatc atcattaccg ataaaggcgt aagccaggcg
    13501 ggattgatcg atcacgtggt gaacagtttc ggcggaacgc aggttgcgat tggcgcgttg
    13561 tatgatgaca cgcccgttga ttcttccatt gtggcggtaa acgagattgc cggaatatac
    13621 cggcagcagg aatgcgatgc gatcatcgcc gtcggcggcg gttccgtgat cgacaccgcc
    13681 aagggagtga atatcgtgat ttcagaagag gcggacgatc tgatgaaatt taccggaaac
    13741 aaccgtctga ccaagccgat gaaacctttt atcgtcgtac ccaccacggc gggaaccggt
    13801 tccgaggtga cgatggcggc ggtcatcgcc aatcccgccc ggaatgtcaa aatgcaattt
    13861 acatctcccc tgattttgcc gaaggtggct attctcgatc ccgtgatgac catgagcatg
    13921 ccgccgaaaa ttactgccgc aacggcaatg gacgccctga ctcacgcggt ggaggcctac
    13981 atcggtccgc agaaaaaccc ggtcagcgat gctttcgctt tttccgccat tgaactcatc
    14041 aaggccaata tcatcgacgc ggcgaaaaac ggcaaagaca gggatgtccg gctggcgatg
    14101 gccaacgcat cgttactcgc cggtatatcg ttctccaatt cgatggtggg catcgttcac
    14161 gcgctcgcgc atgcaaccgg cgggctgtgt catgtgcctc acggcgtcgc caacgcgatt
    14221 cttcttcctc acggtctgga gttttatatt aaaaaatcgc cggatatcgc cgcgcgtatc
    14281 ggcaagctgc tgcggttttt catggaaccg gaagattatg caaagattcc ggtaaatgaa
    14341 agggagaaaa acaccgtggc cgcgatacgc gacatgaacg acagtctgaa caagatctgc
    14401 agcctgccgt tgactctgag ccaggctggg gtaacaaaag ataaactgga agcgatagca
    14461 aatgcggcca tcaacgacgg cgctatagcg ttttgtcctg tcgaggcaag ttatgaggat
    14521 gccctggaaa tattaaaggc ggctttttaa cggatcatat aaccggcatg tatttcgatg
    14581 atcaataaga agggaggaag attatgaatg cacaggtttc aggaacggca gaacatcaag
    14641 catcggctgc aaaagaccag ggtgtcaaaa acgatgtaac ggtttgcacc aatcccgcat
    14701 cgtgcgaagt gatcgccatc taccccatcc ataccctgga agatgtaaaa aatgctgtaa
    14761 caaacgcccg gcgtgcgcaa cccgcatggc aggctctgcc tctgaaaaag aagatcgcct
    14821 atgtaaaaaa atttgcagag tatattcaaa aaaattcggc aaagctggca gaaatcattt
    14881 cgcaggataa cggcaaaacc atctttgatg ccatgtttgc cgaggtgggc cccgcaaccc
    14941 tggcgacatc atattactgc aaaaatgccg ctgaattttt agcggaccgg aaactcaagg
    15001 cgggcaatat catgctgatg aacaagcgca gccgcattgt gcgggtgccg tacggtgtcg
    15061 tcggtattat ttcgccctgg aattacccgt tcgccattcc tttttccgaa gtggtcatgg
    15121 gattgctcgc cggaaacacg gtgattttaa aggctgcatc ggaaacgcag atggtcggcc
    15181 atgcgcttaa ggagtgcatc gactatgccg gtcttcctga acatgttttc acttacatta
    15241 acatgcccgg caacaaggca ggagacgcgt tacttgatgc cggcgtggat aaactgtttt
    15301 ttaccggttc ggttcccatc ggaaaatatt taatgaagaa agcctccgaa aacctgacgc
    15361 ccctggtgct ggagctgggt gggaacgatg cgatgatcgt ttgcgaggat gcggatctgt
    15421 accgcgctgc gtgcggcgcc gtctgggcgg gcatgcaaaa cgcgggacag tcgtgcggcg
    15481 gtgtggaacg aatctacgtg gatagaaaaa tctaccatga atttctggcg gtattgaagg
    15541 aaagggtgga ggggcttcgt gtcggcgacg gcaattgtct gatgacggat atcggcgcca
    15601 tgacgacccg gaaacagatg cggaccgttc aggaacatat cgacgacgct ctggccaaag
    15661 gagcaaaaat attcgcgcaa tccaaatgcc ctcaaaactg caaggggaat tttatcccct
    15721 gcactgtgct gaccgatgtg gaccatacca tgctggtcat gaaggatgaa acattcggtc
    15781 cggttgtcgg cgttatgccc gttgaaaaca tggagcaagc gatagcctta gccaatgatt
    15841 ccaatctcgg cctgacctgc tcggtatggt cgaaaaacag gaaaaaagct gttcagatcg
    15901 gccgccgggt gatggcgggc gccgtcatga tcaacgatca tctgatgagc cacggtcttg
    15961 ccgaaacacc gtggggagga tttaaagaaa gcggcatcgg ccggacgcac ggggatatcg
    16021 gttttgctga aatgactcag cctcaggtta tcgtagacga tatcttgagc gcgatgcctt
    16081 ttgtgcgcag aaacttctgg tggcagccct tcgaaaaatt gcagtacgat ggtatgaaag
    16141 ggctcatcga cttgtttcat ggaaaaggat tctgggaacg ttttgtcgga ttaaataaat
    16201 taggccatgc tttttacaga tcatttgtaa aaaaataagg taataaaaag cgccctgatt
    16261 tcgaaaaaaa atcggggcgt ttttttgtga aaaatgacgc caaaaatgct aaaaaaaaca
    16321 taacccatct tattgatttt atatgtgaat atattaattt aaaatcggtg ttgacagacc
    16381 ggcggtcact gttgtatgaa ccattcctga catatgccat cattggcata tgtcagaggt
    16441 gacattttta ttatttatta ttgaaaaagg tggatgaata atgagtgaaa catttttatt
    16501 gaacggaaaa gagacgccct ttgaacaggg gcagacgatt gtagaagcag cgaaaaacgg
    16561 cggggtcgaa attcccacga tgtgctactt gaaaggctcc actccgaccg gcgcatgccg
    16621 tatttgttgc gtggaagtca agggcgcgcg gtcgctggtg gcggcctgtt ccacgccggt
    16681 aacgccgaat atggaggtgt tcactgaaac cgagctggtt cataaatcaa gaaaaatggt
    16741 cgtggagttc atgctgacca gcggcaaaca taactgcatg acctgtgaag caaacggtaa
    16801 ttgccgtctg caggatttgg catatttcta taaaattacc acaccgcgct ttgaagaaag
    16861 cgaacagctg tatcccacgg aagatgagaa tccttttatc gtccgtgatt tcagccgctg
    16921 cattctctgc ggacgctgcg tacaggcctg taacgaggtt gtcgtaaacc gcgccatttc
    16981 gcagggctat cgcggtagaa aaagcaagat tgttaccggc ggcgatctgc cttacaatca
    17041 tgacgacagc gaatgcgtat tctgcgggca gtgcgtccag gtatgtccgg tcggcgcgct
    17101 gacggagaaa aaggccaaag gtctgggacg cccctgggaa gttcagaaaa tccgcaccac
    17161 ctgtccgtac tgcggcgtcg gttgtcagca gttgctgcat gtcaaagacg gaaagatcgt
    17221 caaggtcacg gccgtcgaag atgcgcaacc caatcaggga cggctttgtg tgaagggccg
    17281 ctttgcttac gatttcattt attccgggga aagattaaaa acgccgctga ttcgagaaaa
    17341 gaacggcgaa ttccgcgagg cgtcgtggga tgaggcgctt gatttggtgg caaataaatt
    17401 caaagagatc atcaagaaac acgggccgga tgccgttgcc ggcgtcagtt gcgcccgcag
    17461 tatcaacgaa gattcttatc agatgcagaa acttttccgc gcggttttcg gtacgaataa
    17521 tatcgatcac tgcgcacgta cctgacacgc tcccaccgtc gccggtctgg cgacttcttt
    17581 cggttcgggc gcgatgacaa attcttttgc cgattacgca aaagccaaaa tgattattct
    17641 ggtcggcacc aatatatccg aggcgcatcc ggtcgcttcg acttttgtga aagacgcggt
    17701 gctggccggc gccaagttga ttgtgattga tccgcggcgg acggatatgg ttgattttgc
    17761 cgacgtacac attccgctga aagtcggttc cgacgtggcg ctgatcaatg ctttgatgaa
    17821 tgttctcatc acggaaaatc tgtacgataa aaaatatgtg gagtcctgta cggagggatt
    17881 tgaggaattg aagaaaaagg tgatggagta cacgccggaa aaagccgcca aaatcagcgg
    17941 tattcccgcg gacacgattc gcgaactggc ccgcacgatg gcttccatca aaccggcgat
    18001 gctgatgtac acgctgggca ttaccgaaca catctgcggc gtcaacaacg ttatgtcctg
    18061 cgccaatctc cagatgcttt taggcaatgt cggcgtggaa ttgggcggtg tcaacccgca
    18121 gcgcggtcaa aacaacgttc agggcgcctg cgatatgggc gcgcttccca atgttttccc
    18181 cggctatcag caggtagcca atgcggaggc ccgggcgaaa ttcgaaaaag cctggaatgt
    18241 caaatcgctt ccggataaac ccggcatgat gatgccgacg atgatgaaaa atttgaccac
    18301 cggaaaagtc aaaggcttct atgtctttgg tgagaatctg gccaatacgg aaccggatat
    18361 caaacatgtt gaacaagagt tgcaatccgc ggaatttctg gtttgccagg atattttccc
    18421 gacggaaacc actcgctttg ctcacgtgat tctgccggct gccgcctgga gcgaaaatga
    18481 cggaaccttc gccagttcag agcggcgcgt caaccgcgtg cgtacggcca gcgtgccccc
    18541 gggagaggcc aaacccaact ggtggatatt caaagaaatt gcaaaacgtt tcggtcacga
    18601 gtgggcatcc aacagcgctc aggaaatatg ggataacgaa gtatccgtgc tggcgccctc
    18661 tatggcggga atcaagtatg cccgtctgga aggtgacggt ctccagtggc cgtgtcctac
    18721 cgccgatcat ccgggtaccc cgactctgca caaaggcggc aagttcaccc gcggtctggg
    18781 tatgctcaaa gccatcgact atatcccgcc ggcggaagtt cccgataagg aatacccgat
    18841 ggtgctttcc accggccgtc gcctgtatca ttatcatacc cggacacaga ccggacgatc
    18901 cgaggggatc aacgatctgc tgggcgagga aacagccgat atttctccgg ccgatgccga
    18961 ggccaagggg attaaaaacg gcgagaagat tctggtgaag tcccgccgcg gcaaggttga
    19021 ggtcaaagcc agggtaacag aagaagttcc gcaagggctt gtctggatgg ctttccattt
    19081 ccgcgaagct tgcgcgaact ggctgaccaa tcccgtttat gatcccgtca cgcagacggc
    19141 ggaatacaaa gcctgtgcgg tgaaaattga gaaactgaaa taaataggga atcaaagtgt
    19201 cccgctttca aagcgggaca ctttttttgt gcagtgatta ttgtccgaga aacaacatcc
    19261 gattgatctg ttaaaagcca tcccgaaaaa caaatttttt attatgttat tattgacata
    19321 atgttatttt taatatagag tcccctcgta tttacatcag aaagagagga tcttttattc
    19381 atgaaaaaat caaatttcaa acaggtcatc acaaaaattt tattgacagt gatcggtgtg
    19441 gctttttttg cagggctgtc cggtcaagcg tttgcggaag aaaaaatttc cgttgcggtt
    19501 gcggcaaact ttatttcagc ctttaaagat atgggggccg atttcgaagc caagaccaaa
    19561 attaaagttg aaggcacttt ttcctcatcc ggaaatcttt acagccagat caccaacggc
    19621 gcgccctacg atctgttgtt atcggccgat gaagaaagac cggccaaact caacaaagac
    19681 ggcttggcgg ataatccttt tatttacgcc agagggcagg cgattttatg gtctgccaat
    19741 aaggaattct gtaaggcaaa aacctggcag gatgccctga aaaatgaaca gatcaaaaaa
    19801 ctggccatcg ccaatacgca gaccgcgcct tacggaaccg cagcgcagaa agcattggaa
    19861 aaggccggga tgtggaccga cgtgcagagc aaactggttg ttgcgcaatc gatcgcacaa
    19921 tcgtttcagt atgcatcaac ggagtcggtt gacgccggtt tctgtgccct gtcggctacg
    19981 gccagtcctg aagggaagaa gggctgtttt tatgtcctga atgaggcgcc ggaaattatt
    20041 cagtcggcct gcattctgaa aagaacaaca aaccgcgctg cggcggagaa atttgcggag
    20101 tatctgcgat ctcccgcggc acaggaaata aaagttaagt acggttatcg gtaagtggat
    20161 tatcttgcaa tttatgtgac attaaaactg gcgctggtga cgactgttgt tttgatggtt
    20221 atcgcggcgc cggttgctta cgctctggcg tacaatcgtt tttccgggaa atcctttctg
    20281 gaagcactga tttatctgcc gatggcgctg cctcccacgg tgatcgggtt ttatctgatc
    20341 atcctcatgg gaccgaaagg ttttgtcggc aaaatgtggc agatgcttac aggcggatcg
    20401 ctgcttttta cgtttatcgg cattacgatg gcgtccatca tttacagtat cccgtttgcc
    20461 gtgcaaccga tgaaagccgc cttttccaag atagaccgcc gtttgctgga atcggcctat
    20521 gttctcggtt tatccaaaaa ggcgacgttt ttccgggtga ttattcccaa ttcggtcagc
    20581 ggcattgccg ccgccgccat tctggttttt ctgcattcca tcggagcatt cggcgttttg
    20641 ctgatggtgg gcggcagtat ccccggcgaa accaaggttg cctcgattgc gatttacgag
    20701 gccgtggaaa tgatgaatta caaggccgcg ggattgatcg cgctgagttt cattcccatc
    20761 agttacgcct ttttgctgtt agtgaataag ctcaatgaag gagcgcgttc atgagtattg
    20821 aagtttcttt taaaaagaaa ttaaaatatt ttgatgtgga catttcattt tcctgcgcga
    20881 aaaaaaacat ggtcgtgatg atcggtccgt ccggcggcgg caaaacaacc atcattcgca
    20941 tgctggcggg tctggaaaaa ccgaaagaag gaaaggttgt attcggcgat gaagtctggt
    21001 ttgattctct gcgccgcatc gatgtcccgc cgcagaagcg gcgcctgggt tatgttttcc
    21061 aggattataa tctttttccg catctgaatc tctttgacaa tgcggcgttt gccgcggtgg
    21121 ataaaaaaga agttgaagat cttttcggga ttttcagaat cggccatttg aaaaaacgca
    21181 aaccgcacat ggtgtcgggc ggcgaacggc agcgctgtgc aatctgccag gcactggcga
    21241 ggcatccgcg tattctgctt ctggatgaac cgttttccgc gctggatgcc attacgcgcc
    21301 gcggtctgcg cgaagagctg aaaaatctga agggcaaaat gtccttcccg attatttatg
    21361 tgacgcatga cattacagaa gccttgtatc tggccgatga acttctgccg gtggtggacg
    21421 gcaaaatcga cgatcaatgg ctgcagcgga tgatgacccg cgagccgtcg tcggaagtcg
    21481 ccctgcgcgc cgcgcgcgaa ccgaggctga cattagtata ctagattagc tttttatgaa
    21541 tatgtctact gtgtcatttc gaagcgaagc gagaaatctt aatgatcaat attcaagata
    21601 tctccccgga agagttggac tatagcctct ggttgatatg acaaatatat cgaagaagcc
    21661 gctcccgatg aatcgggacc gacaaagaca gcgctttgat ttggaaataa tttaaggagc
    21721 aaaatatttt atgaacatct gtacctattc ctacgaagaa tacatccatc tggttaaatc
    21781 attccatgga acactggcgc ccggcctgct gatcggcggt tttatcgttg atctggccat
    21841 gaagaatctg ccggaaggag aattctttga tgccgtttgc gaaacaccgg tctgtctgcc
    21901 cgatgccgtg cagattctca cgccctgcac catcggcaat ggctggctta gtatcgtcaa
    21961 ctttggccgg tttgccgtga cgctttatga aaaatattca ggtaggggtg tgcgggttta
    22021 tctggatacg gaaaaattga ataactggcc ggaagttcgc gactggtatt tgaaaaagaa
    22081 gaaaaaggat cagcaagacg ccgaggtgct ttttgcgcaa atcaaggaag ccgggcatca
    22141 attgttgagc gttcagaagg ttcaggtgga acccgaaaaa gttcgtcgca aaaaaatggg
    22201 acccgtcgga atctgtccgg cctgcggaga ggcctatcct ttaaaagacg gcggaaagtg
    22261 catgaattgt cagggagcga ccccgtattc cagcgttgtt ccggtaaaat aatttaacaa
    22321 tggggaacgt tgctatgcta aaaacaggta tcacgcttag tcgtcttttt gaaaaaactg
    22381 aaccggctgc caggaatcga cagattgccg atttacgcat cggcttaagt tatgtcggaa
    22441 taaaactgga taacggggtg gcgggacttg ccgccgtcct gccggggagt accgcacgcg
    22501 gctgcacggt tttaaaggaa gcggggacat acatcggatc acccgcggtt gaaacattac
    22561 aatatcttgt ggaggcaaaa gacgctctgc gcagggcgat cggtgtggcg acggccaacg
    22621 cgcttgtttt gcctgaaagc ggaaaaaccg aagaccgcga agcgaccacg tacctgaatc
    22681 tccaacccgg tgaaaaggtg gccatggtcg ggctgtttgc tcctctggtc gaacgcatcc
    22741 gatctaccgg cgcgacactg accgtggttg aaaaaaatcc cgagcgtctg gaagtgctct
    22801 cggcagagga aaaacaaaag gcgctcaaag actgcgatgt tgcgattatt accgcaacga
    22861 ctcttctgaa tggtacgttt gaagaaacaa tcggcttgct gggccggccg agatcggttg
    22921 ttctgatggg accctccacg ccgctcgtgc ctgaaatctt ccacgacacg cctgtcaccc
    22981 agttgggcgg ttcaatcgtt gccgatgccg caaaagcgct gcaggttatt tccgaaggcg
    23041 gcggcacgcc ggcgctcaga cactatcttc gttttgtgaa tctgctcctg cctgacaaga
    23101 gccgctaaga ttttatatct ttttgaactg ggctatctgg cgagaaatcg cggcgaggtt
    23161 gcctttttca tcccagactt ccccgtcggc ttccagaatt ccacaggtaa tgaaacgcgt
    23221 ccggagactg catttgagcc ggtccgtttt cggcagattt ctgacgttca cggaaagctc
    23281 gatcgtcgga acccaggcgg tggcgccctg ggaagccatg gccgccgggg gcatcgaatc
    23341 aataatcaga aaaagcgaga agagatcgag aggccggccg tcacgaaatc tgaaatagcc
    23401 cttgttcaga gaaacgtccg tcagttctcc tccggtcatc catccggcgc atgcgggatc
    23461 gagacggagg tcgaggtttt ggaaaagcgt gtatgacggc ggcacagaca tcggcacaca
    23521 ttcctcgatc ggagcaagct caggggcttt tgactcatag cgggttatcg cacaatcatt
    23581 gttgtctgaa acgaatgtgc cgagcgccct gattttctca tcgccttcct gtataagacg
    23641 agcctcaaaa cgggtaaact gccgcgaacg ggtaatttcc gcaatatgaa tctcgatctt
    23701 gccgggaacg catcgggaca gataattcgc ggtgacaacc ggcgtggcct tcttgtcgct
    23761 cttccggagc atcgcgttga cgatcatggc catgaggtag cccccgttgg ggttgccgtt
    23821 gaccgaccag ttttccgaaa tcgttccggc aaagcgtgaa tgatccgatt cgctaatttg
    23881 tatgtcttta tcaaaaagat tcatgaacat ccttttcctt tatcaggagc attccccccg
    23941 gtatcacaac tccttggccc tttaccgcac aaagcgcctc gggatcaggt cctcctgatg
    24001 aattcaggcg gcctggcttc ctgaagggcg atcgcttcta ccgggcagac gatgacgcaa
    24061 ttgccgcagc ccatgcattt gttgcagtca atgacggccg tgtctccgac ggtgatggcg
    24121 ttaaatgcgc acttctcttc gcacgcgccg cagccggtgc aggtgtcggc gtcgacctgc
    24181 gccagaaaac gggagggcgc aaacgatgca tcgaaacccg cctgattgtg gagaaacatg
    24241 gccgtgcaac aacaggggca gcagttgcac atgaacatat cttccttgac gttgttgcgc
    24301 accgtatgaa ccaggcctgc ctcctccgcc acagccagcc gccttaaggc ttcatccgta
    24361 ctcagaacct cgcccacgcc gcggtcgatg gcggcctcgg caaagccgtt gatctgcatg
    24421 cagaggttga cgccgtcagg cgcgatacag tctttcgtct tgcccaggtg ttttgcctgg
    24481 gtgcggcacg ggcagggaat ggccgtgatg atccgggcgg aacggatgat gcttctggcc
    24541 gagtcggcgt cgaggacacg cgaagaattt tccacggtct cctcaatcgg aatgacccgg
    24601 agcatgggtg tcaggttcag cttgttaaag acgggcacga tcctgggcat ttcttctttg
    24661 ataatgattt cccaaagctc gaagagctct tcggggcagt ccggcatgat gaccaccgca
    24721 tcccgcaact cgatcatggc cgggtaaaga cggtaatagt ctcccttggc cagcacccgg
    24781 ctgatggctc ctttgcgcag cagtttctgc gcgatctgac gcacccgttc ttcgttcagg
    24841 cccgtcctgg cggcaatgtc cgccacagag cccggcaagg cctccgccac ggcgacgctg
    24901 tccgcattgt cgtaaaggat ggcgaagatc ttcgccatcg tctcagatcc ggggaatccg
    24961 agtttttcag catattgttt gagcgtgtcc ggcatattcg taaaaatctc cttcctgttc
    25021 aagcgaccgt ccggtcgttt ttatcggttc ggcgctgtgc gttaatgatg atcaacagcg
    25081 cttcattcgc cgtctgcgta tcacgcagcc tcgacccggc atcgcacata acggtgcatg
    25141 ggggttccaa actgatcgcg gttggtagtt ttggtcaaac gattcacgtt ggcgccgtat
    25201 ttgacgccgt tgtaaatcag accgaaacca tgaggcatcg tgacatgtcc ggggcgcacc
    25261 gtctccgtga tttccagttc aatctcttcg ctgcccgctt ccgtggtgac ccggaccggc
    25321 tgtccatcct ttaaatttaa gactgctgcg tcgtcgggat gcatggccag cgtgcagggg
    25381 cgtttgcctt cattccaggc gggattgcgc atcagcgtgt tggcgttcat ggaggtgtgc
    25441 cgtcccgcca tcagaacgag cgggaattca gcgggcatga ccagtgccgc ctcttccctt
    25501 tgtgcatcaa tgcttttcag ttcatccagc aattccggaa tgaagagatt gattttgccg
    25561 tcgggtgttt tgatttccgc gaagttattc ccttcatcgg ttttccccac ccagaggcct
    25621 tccggatgat ctaaaatttt ccggaagatt tcttcgccca gcgttggtcc cggcgtgaat
    25681 ccggctctgg cggcgcttgc ctggaaggat tgcggagcaa cctgtaacat tccccatagg
    25741 gcggcgaggt gaacggaccc catcgccttg cccagtgttt tgcccagtat aaacggcatg
    25801 gttttcatcg ccttcggttc cgtcatcgca tattccatca gcgcctgtct gaaggcggca
    25861 tgactttctt cggctgtttt ataaagtttt tccggaatcg gcggaatcag tcccagcttg
    25921 tccgccagtc gcagatcgat ttctccctgt tccagcggtt cgccctcggg tgcgatgatc
    25981 ggccggcgca tctgaaagaa tatctccgga taggtaaagg ggaagaaagt gccgtcccag
    26041 gattcataac cggatcggga gggtagtacg taatgggaaa gagcggccgt ttcggtcatg
    26101 gccagttcca ccgtcaccag caggtccaga cgctgaaacg ctttttcata cgccgttgtg
    26161 tccgcatacg atcgcagcgg attcaaaccg gacacgagaa ccgcgcgcag tctctcggga
    26221 tgatcggaga gaatttcttc cggcatgacg ttgggcggaa aacatccgca gaccgggaag
    26281 gcgttcgtcg cgacggttct ccaggttttc tgatttcttt catcgctgtt cggaccgaag
    26341 ggcacgatgt gaccgggaat gatattgccg ccgggaacgc acagcctgcc gcagatggat
    26401 tgaagcatga cgatcaggta ggaattcagg ccgctgtgac gtcccatgaa aataccgaga
    26461 tcgtaacgca aagaggattt gcgtttggcg tacgcttggg tgaccttata aacctgatca
    26521 aaatcaagtt cgcagacttt gagcgcggcg cggacatcaa aattttcaaa cagggatttg
    26581 acctgttcaa agcccgctgt gtgattttcc atgtaatctt tattgatcca gccttctttc
    26641 agaatgatgg cgatcatgga tttgagcaac agggcgtccg tgccggggcg aatccgcaga
    26701 tggatatccg cgatcttcgc ggtttctgat atacggggat caacgacaat cagcagtttg
    26761 tccgggtctt tggagagacg ctgaagctgg cgcggcgcct gcgggatctg gtggctttcc
    26821 atgccgttcc agccgaagat cagcagaacg tccgtattct tgacatccgg accgtcgtgc
    26881 aggtattgtt tcccgtgcgt gcgtccgcac acccagaacg cgccggaaaa ttcctgtgcc
    26941 agagcgctgt actggtactg cgagcccagt gtgcgcatca gggagacgcc gaacgcgacc
    27001 tccaggtggc agctctggtt tcctccgccc atataggcaa aggaccgggg gccgtactgg
    27061 ccgacaatat ctttaagctt cgcggcaatt tcatcaatgg cctgatccca cgtaatccgc
    27121 tcgaagtgat tgccgacttt tttcaaaggg tatttcaagc ggtcggcgtt atgctggtga
    27181 aacgcgacat tcatgccctt gcgacaaaag tatccttcgc ttttaggatt ggatttgtcc
    27241 ggtttgacct tgacgatgcg gttgttgtcc acgtcgactt ccagaccgca gttctgcgcg
    27301 cacagaacac agccggtttt atgcagggca cccatatctg ctcctttcgt ccggagtgta
    27361 accggatgct ttgaatgcgt tttggtcagc gggcagcgcc cagacgcagt tttttgataa
    27421 tatcgaacaa atgacgtttt tcttctttcg tcaggctttg caaaaattca agattggccc
    27481 cggcgatcac gcggatcgct tctttgctta atgcatttcc cttaagcgtt aaatgaatat
    27541 gaatggaacg ccggtcttca ggattgtttt tgcgttcaat aaattcagcc gcttccagac
    27601 ggtccagaat gccggtgagc gttgcgctgt ccagcccggt tttctttccg agttctgcgg
    27661 atgtgatttg atcttcctga tgcagaaaac ccaaaaccag cgcctgcacc ggggtgagat
    27721 tgagttcgga tactttttga gaaagaaagc ggctggccag ttgattagct ttggccagtt
    27781 gaaaaaaaat acaatccttt tcctgcatag ggcttattat cctcgcatac taaatacttg
    27841 tatgcgagga taataaggaa aaagagtttt gtcaagtgca aagaatcgaa tttccatcgt
    27901 tatgaaaatt tgcggtttga acggttgggg ttttttaatc ggcacagaac atttcctttt
    27961 catttcagcg gctgacttta agtcaattac ctctgaaatt atcgataaaa atttaatttg
    28021 acccaaagtc atttatgatg aggagtcccc atgtcgatct ggataggcgt tcaatcttac
    28081 aatacttttt tattatgtca aaaatgacat aaggcattga aattattagt tttttacgtt
    28141 gacaacatct gctgcttgat gtataaatgg cccgtcataa atgcgttatt ctttggaaaa
    28201 aacgttgtgc tcaatccaag gcctgcttct tccaaaagct tttcctgaaa atgcaaattc
    28261 aaggaacaaa tcacacagga aagccatagc gcaaaaagaa tatttcaata ttgatgaaag
    28321 gagaaaaatt catgaacgtc agccgaagag gtttcttgaa aatttcaggt gccgtggcag
    28381 cggtcagcgg attgggcatc agtctcaaat ccatagccgc gcatgccagg gaactgaaaa
    28441 tcatgcatgc caaagaaacc accaccgttt gtccgtattg ttccgtcgga tgcagcatca
    28501 tcgtgtccgt gcgtgacggc aaggttatca acacagaagg tgatcccgac agccccatta
    28561 accgtggagc gctgtgcagt aaaggcggtt cgatctatca gatggctgtc aacgagaacc
    28621 ggctgggcaa accgctttac agagcgccct acgccagaga atggaaagag gtggaatggg
    28681 aatgggcgat tgacaaaatt gcccagaacg tcaagaaaag ccgtgacgcc agttttaaaa
    28741 aagtcaatgc caagggcgaa gttgttaatc gcacggaggg cattgcctct gtgggaagcg
    28801 cggcgatgga tcttgaagaa tgtttcacct atcaaaaatt cttaaggggg ttgggtctgg
    28861 tctatattga acatcaggcc cgtatctgac acagcccgac tgtaccggct ctggcagagt
    28921 cgttcggacg cggcgcaatg accaaccact gggttgattt caaaaatgct gatgtgattt
    28981 taatcatggg aagcaatccc gcttccaatc atccggtgtc atggaaatgg attcaggaag
    29041 ccgtcgataa acgcggagcg aaaattatct gcgttgatcc ccgcttcacc cagtcggctg
    29101 cgaaagcgca cctttacgca ccgcttcgtt caggaacgga tatcgctttc ctgggcggca
    29161 tgatcaaat
//
LOCUS       DAOSME010000028        27247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2256_length_27247_cov_21.891770, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000028 DAOSME010000000
VERSION     DAOSME010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 27247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27247
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2256_length_27247_cov_21.891770"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..300
                     /gene="secE"
                     /locus_tag="PLG98_05835"
     CDS             85..300
                     /gene="secE"
                     /locus_tag="PLG98_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOE32810.1"
                     /translation="MEDFKEKVKVTIQKITQFLKDAKMELKKVTWPTPKQALASTAVV
                     IILVFIVSLILGIIDFALAKAVKLILG"
     gene            305..835
                     /gene="nusG"
                     /locus_tag="PLG98_05840"
     CDS             305..835
                     /gene="nusG"
                     /locus_tag="PLG98_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOE32811.1"
                     /translation="MAFKWYVVHTYSGYENKVKISLEERIKTTGTQAYFSDILIPEED
                     VVELISGEKKSSKRKFFPGYILVRMDMNDEAWHVVKDTPKVTGFIGSKDKPSPIPDKD
                     VEHLKIRIDEGTLKPKPKFKFDVGDHVKIIDGPFTNFDGIIDELKPEKSKLRVIVSIF
                     GRPTPVELDFIQVTQG"
     gene            891..1316
                     /gene="rplK"
                     /locus_tag="PLG98_05845"
     CDS             891..1316
                     /gene="rplK"
                     /locus_tag="PLG98_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOE32812.1"
                     /translation="MAKKIIANIKLQIKAGKATPSPPIGPALGQHGVNIMEFCKAYNA
                     MTQSQEGMIIPVVITVYADRSFTFITKTPPVSVLLKQAAKIAKGSSDPKRDKVGSVTA
                     SQVKDIAELKYKDLNAVNIEGAINIIAGTARSMGIEITG"
     gene            1336..2034
                     /gene="rplA"
                     /locus_tag="PLG98_05850"
     CDS             1336..2034
                     /gene="rplA"
                     /locus_tag="PLG98_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOE32813.1"
                     /translation="MLRRGKRILAAKAKVEPAKKYTLKEAAEIVVSTAGTKFDETVDA
                     AIRLGVNPAHADQMVRGSVVLPHGLGKTVRVLVFAKGEKEKEALEAGADLVGNDEVIE
                     KIKGGWMEFDRVIATPDMMGSVGKIGKILGPRGLMPNPKVGTVTFDVANAVKELKAGK
                     VEFRVEKAGIVHSPVGKVSFGAEKLRENVSALLETILKLKPASSKGTYIKSISISSTM
                     GVGVRIDPLDIKAA"
     gene            2356..2880
                     /gene="rplJ"
                     /locus_tag="PLG98_05855"
     CDS             2356..2880
                     /gene="rplJ"
                     /locus_tag="PLG98_05855"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOE32814.1"
                     /translation="MDRKTKEQIVSELQKKLKEANLGILTSFSGMNVEKMEALRNELR
                     KSNAEWKVVKNTLLRIASEGTDFSILADHFKWPVAIVLSYKDPVAPAKVLIDFAKKNA
                     ELEIKVGVLDKKLLTRNDLTVLAELPGKDVLLGKLVSVMAAVPTSFVRVLNGVPGSFV
                     QVLNAYCDKKKSLN"
     gene            2906..3295
                     /gene="rplL"
                     /locus_tag="PLG98_05860"
     CDS             2906..3295
                     /gene="rplL"
                     /locus_tag="PLG98_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOE32815.1"
                     /translation="MSDKITKEDVVKFIEEMTVLELSALIKELEDKFGVSAAAPVMAA
                     VAAPGAAAAAGPVEEKTEFDVILTGFGAEKIQVIKVVRAITNLGLKEAKDLVEGVPKP
                     IKEGASKDEAADIKKKIEEVGGTVEIK"
     gene            3335..7465
                     /gene="rpoB"
                     /locus_tag="PLG98_05865"
     CDS             3335..7465
                     /gene="rpoB"
                     /locus_tag="PLG98_05865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOE32816.1"
                     /translation="MGDFRKNFGQVRKILDIPNLIDIQTESYEKFLQKDIAPVKRGAF
                     GLQGAFKSVFPISDFSGKCSLEFVSYKLGDIRYDVKECVQKGMTYAAPLKIVVRLVVF
                     EADRPAEGKGAGETTEAKQIRDIKEQEVYFGEIPLMTENGTFIINGTERVIVNQLHRS
                     PGIFFDHDKGKIVASGKLIYSARVIPIRGSWLDLEFDSKDILYVRIDRRRKMPVTILL
                     KALGNSTEFILNYFYNVNTVINDNKKIYFAVDESLVGKKSPEDIKDSKSSELIVKKGR
                     KLTKPLIKRIMEAGVKRTAINENELIGKIPSRDVHDPETGEIVFRCNEELPANCLDII
                     REKNIRELKLIHIDEDMDNASIRDTLLLDKIDTTEDAIMEIYRRLRPSNPPTPETADK
                     FFKSLFFEPETYDLSNVGRAKMNYKLHMNVPTDNTVLSNEDILSAVKYLIDLKNGVGD
                     CSVDDIDHLGNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLINAKP
                     VSAVVNEFFNSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHY
                     GRICPIETPEGPNIGLIVSLSTYARVNEFGFIETPYRKVENGRVSDDVHFVTAIEEEN
                     QIIASADRPLSKNGKFAEELISVRKGGSYISVEPEEIKMMDVSPTQLVSVAAALIPFL
                     EHDDANRALMGSNMQRQAVPLMNPEVPVVGTGVESVVARDSGAVVLAKRSGVVESVDA
                     SRIIVRADRMDEDGIDSGVDIYNLAKYQRSNQDTCFNQKPIISVGQRVHKKDILADGP
                     AIDNGELALGRNVMVAFMSWGGYNYEDSILVSERVVKEDIFTSIHIEEFETMSRDTKL
                     GKEEITRDIPNVGEEVLKNLDESGIVCIGVSVKPGDILVGKITPKGETQLSAEEKLLR
                     AIFGEKASDVRDTSLRVPPGVEGTVIDAKIFTRKGAESDSRSQAIEADAIEQIAKDRD
                     DEIRILTETIKKDITKLVVGKTAATRIGDAKKKTVLKKGDKITKEIWNDLPFSSWKDI
                     TIAENDGSEEKIAGLIASLERKIDFVSAHFEEKMEKIKASDELPPGVIKMVKVYIAIK
                     RRLSVGDKMAGRHGNKGVLSRILPEEDMPYFADGSTVDIVLNPLGVPSRMNVGQILET
                     HLGWAAKSIGEGLNELLEQQRAGLSHIKDELKKVYSSPDFDKFIDQASTEDIKRFVGR
                     LKKGMLVASPVFDGCPEDQIREMLKRTDLPETGQSILFDGRTGEPFDQEVTVGIIYMM
                     KLHHLVDNKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGAAYALQEFL
                     TVKSDDVAGRTRIYESIVKGEHNFESGLPESFNVLVKELQGLGLDVELVEESDSLS"
     gene            7546..11640
                     /gene="rpoC"
                     /locus_tag="PLG98_05870"
     CDS             7546..11640
                     /gene="rpoC"
                     /locus_tag="PLG98_05870"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOE32817.1"
                     /translation="MEDVFSYFEKPKNLVKFNAMQISIASPDKILSWSHGEVKKPETI
                     NYRTFKPEKDGLFCAKIFGPVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERM
                     GHITLATPVAHIWFLKSLPSRIGNAVDLSLKELEKVLYFESWIVLDPKNTPLKKKDLL
                     SDEEYEEAREQYGNDGFVAGIGAEAIRQLLEEIDLEKLYEELRTEIVTSVSDTKKKKL
                     VKRLKVVEALRKSGNKPEWMILTVLPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLQELNAPEIIIRNEKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVVTVGPDLRLHQCGLPKKMAIELFKPFIYNRLLEKGYV
                     TTVKSAKKMVEKESAEVWDALDEVVREYPIILNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCTAFNADFDGDQMAVHIPLSVEAQTEARVLMMSTNNILSPANGSPIIIPSQDIV
                     LGIYYLTRAKTGVKGDGMIFAGPDEVRAAIDNGVIDLHARIKVRIDSELKETTAGRII
                     LSEIVPEQISFDDINKLMNKKELTSLIDHCYRLCGDKTTIILADKLKDLGFKYATVSG
                     LSFAVGNMIIPEQKKKIVLQADKDVLKIQRQYMDGLITDGERYNKVVDIWAQATEKIA
                     SEMLKGISTEDVPAAEGKKRKIESFNPIYMMADSGARGSGQQLRQLAGMRGLMAKPSG
                     EIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDCIIT
                     EHDCETVDGVYVSALMEGGEIIETAGERVLGRVALQDVKDPFSGEVIVPANQMIDETL
                     AEKIDNAGIEKVHIRSVLTCRSKHGVCAMCYGRDLAHGHLVNIGEAVGIVAAQSIGEP
                     GTQLTMRTFHIGGTAKFDEHSTLEARHDGIVKFVNLSTVKTKEGDLVVMKRNGEVHVL
                     DEQKHVRGKYVLSYGAHLKVTDNSEVKKGQLIAEWDPFSIPILAEVDGTIKFDDITEG
                     KTMQEQVDEVTGLSRKIIVEFKGGDLRPRVSIKDEKGKTAKLADSNAVARYLLSVGVN
                     LVVSEGDQVRAGDIIAKIPRETTKTKDITGGLPRVAELFEARKPKDFAVISEISGVVS
                     YGKDAKGKRKIVVTPEVGEEKEYLIPKGKHISVQEGDVVSPGDALMSGVNNPHDLLSI
                     KGEKELARYLVDEVQEVYRLQGVKINDKHMEVIVRQMLRRVKVMDPGDTAFIADEKIE
                     RYRFQEENEKIIAQGGRPAIGEPMLLGITKASLSTQSFISAASFQETTKVLTEAALSG
                     KTDYLRGLKENVIMGRLITAGTGLVMYRKLGIALENENAETAAE"
     gene            11752..12123
                     /gene="rpsL"
                     /locus_tag="PLG98_05875"
     CDS             11752..12123
                     /gene="rpsL"
                     /locus_tag="PLG98_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOE32818.1"
                     /translation="MPTISQLVRKGRAKIKRKTNSPALAGSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHVIRGTL
                     DTVGVQDRKQGRSKYGAKKPS"
     gene            12138..12608
                     /gene="rpsG"
                     /locus_tag="PLG98_05880"
     CDS             12138..12608
                     /gene="rpsG"
                     /locus_tag="PLG98_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOE32819.1"
                     /translation="MPRRREVEKRDILPDPKFNNKLVAKFINSLLKRGKKSVAESILY
                     GAFDIIEKRVKEQPVDFFEKAVNNVKPVIEVKSRRVGGSTYQVPTEVVPARRTALAIR
                     WLISNAQERTEKTMREKLASELIDAANNRGGAIKKKEAVHKMAEANKAFAHYKF"
     gene            12665..13858
                     /gene="tuf"
                     /locus_tag="PLG98_05885"
     CDS             12665..13858
                     /gene="tuf"
                     /locus_tag="PLG98_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOE32820.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKYLAKKGFAEF
                     RAFDSIDNAPEEKERGVTINISHVEYETEKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPYIVVFLNKVDLVDDPELLDLVELELRELLTQ
                     YEFPGDNIPIIRGSALKALEADDINSPDVKCIAELMDAVDNYVPEPKRDTDKPFLMPV
                     GDVFTISGRGTVVTGRVDRGIVKVGEEVEIIGIRETIKTVVTGVEMFRKTLDEGRAGD
                     DVGLLLRGIKREEVERGQVVAKPASITPHTKFEAAVYVLTKEEGGRHTPFFSGYRPQF
                     YFRTTDVTGVATLPEGVEMVMPGDNVKMSIELITPIAMEEQLRFAIREGGRTVGAGVV
                     SKVIA"
     gene            13878..14186
                     /gene="rpsJ"
                     /locus_tag="PLG98_05890"
     CDS             13878..14186
                     /gene="rpsJ"
                     /locus_tag="PLG98_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOE32821.1"
                     /translation="MKEQKIRIRLKAYDYKLLDRSVEEIVETAKKTGARVAGPIPIPT
                     EINKYCVNRSPHVDKKSREQFEIRTHKRLVDIVGPTQATVDALMKLDLSAGVDVEIKA
                     "
     gene            14275..14901
                     /gene="rplC"
                     /locus_tag="PLG98_05895"
     CDS             14275..14901
                     /gene="rplC"
                     /locus_tag="PLG98_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOE32822.1"
                     /translation="MSKGIIGKKLGMTQVFADDGSAVGVTAIEVEPSVVLQVKTKDKD
                     GYDAVQLGYGRVKQKNVTRPLQGHFNKANKGFFRFVREFPAHSETYEVGQEISADIFA
                     MGDYVDVVGTSKGKGFQGVVKRHGFGGGRATHGSMFHRAPGSIGASADPSRVFKGTKM
                     GGHMGNVRKTVQNLKVWQVRSERNLLLLEGSVPGSRNSFLLITKAKKK"
     gene            14930..15553
                     /gene="rplD"
                     /locus_tag="PLG98_05900"
     CDS             14930..15553
                     /gene="rplD"
                     /locus_tag="PLG98_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOE32823.1"
                     /translation="MAVADVLNIEKKKVAEVDLNDAVFGADVNEAVIYDVVKMQLASR
                     RSGTASTKTRSDVSGGGKKPFRQKGTGRARAGSSRSPVWRTGGTVFGPHPRDYSFSVP
                     KKIRKKALISALSLKFKENKMIILKDFPMEKISARGFQKVVDLFDLKKALFVLDDNNE
                     ILMKSSRNIKNVKMIRSEGINVYDVLNHEHLILLEPSVKKIEGALLV"
     gene            15553..15840
                     /gene="rplW"
                     /locus_tag="PLG98_05905"
     CDS             15553..15840
                     /gene="rplW"
                     /locus_tag="PLG98_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOE32824.1"
                     /translation="MDRSKVVKRILVTEKSTMARDASNKYFFEVDRKANKVEIADAVE
                     KLFKVKVMDVRVMHVLGKKKRMGRTVGQKSSWKKAVVTLAAGNSIEFAEGV"
     gene            15868..16692
                     /gene="rplB"
                     /locus_tag="PLG98_05910"
     CDS             15868..16692
                     /gene="rplB"
                     /locus_tag="PLG98_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOE32825.1"
                     /translation="MAIIKYKPTSPGRRFQSVSDFAEITSTEPVKRLLKPLKKKGGRN
                     SYGRVTARHIGGGHKRKYRVIDFCRNKADIPAKVASIEYDPNRTARIALLNYRDGEKR
                     YIVAPAQVNVGDVLYSGEKADIKPGNAVPLKYIPLGSLIHNVELKVGRGGQLIRSAGA
                     YGQLMAKEGSYAQVRLPSGEVRKIFIECMATIGQVGNNEHENISIGKAGRTRWLGKKP
                     HVRGVVMNPVDHPMGGGEGKSSGGRHPCTPWGIPTKGHKTRKNKRTDKYIIKRRGK"
     gene            16695..16973
                     /gene="rpsS"
                     /locus_tag="PLG98_05915"
     CDS             16695..16973
                     /gene="rpsS"
                     /locus_tag="PLG98_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOE32826.1"
                     /translation="MARSIKKGPYIEEKLLAKIRKMEAEGTKNVIKTWSRRSTITPEL
                     IGYTFAVHNGKKFIPVYVTENMVGHKLGEFAPTRVFFSHSGDRKTKVS"
     gene            17016..17348
                     /gene="rplV"
                     /locus_tag="PLG98_05920"
     CDS             17016..17348
                     /gene="rplV"
                     /locus_tag="PLG98_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOE32827.1"
                     /translation="MEAKAVAKYMRMSPQKVRLVADLIRGKNVQEARNILLYTRKYAA
                     GVVANVLKSAVANAAQNPNIDENILYVKEIYVDQGPALKRWRARAQGRAAGIKKRSSH
                     ITVILDEI"
     gene            17360..18001
                     /gene="rpsC"
                     /locus_tag="PLG98_05925"
     CDS             17360..18001
                     /gene="rpsC"
                     /locus_tag="PLG98_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOE32828.1"
                     /translation="MGQKVNPVGLRLGYIKKWQSVWYTDKKYSDVLHEDLQIRKYLKK
                     KLYHAGLSKIEIERAANKAKVNIYSARPGVIIGKKGSEIEKLKSEIERFSQSEIIINI
                     LEVRKPEVDAQLVAENVAMQLEKRVAFRRAMKKCVGYALKFGAKGIKIACSGRLGGAE
                     MSRSEWYREGRVPLHTLRADIDYGFTEAKTAYGLIGVKVFIFHGEVLPTKNEK"
     gene            18080..18502
                     /gene="rplP"
                     /locus_tag="PLG98_05930"
     CDS             18080..18502
                     /gene="rplP"
                     /locus_tag="PLG98_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOE32829.1"
                     /translation="MLMPKRVKYRKLQKGRMGGKATRGSSIAFGEYGLQAAECGWITS
                     RQIEAARVAMTRHVKRGGRIWIRVFPHKSVTKKPAETRMGKGKGAPEGWVAVVKPGSV
                     LYEMEGVSKDVAREAFRLAAHKLSIATKFLSRDIVDEN"
     gene            18492..18686
                     /gene="rpmC"
                     /locus_tag="PLG98_05935"
     CDS             18492..18686
                     /gene="rpmC"
                     /locus_tag="PLG98_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOE32830.1"
                     /translation="MKIKEIRALDSNGLKQKIAELSEELFRLKIRHASGQLESTVMLS
                     KIRKDIARVNTVLKEKEAAS"
     gene            18688..18945
                     /gene="rpsQ"
                     /locus_tag="PLG98_05940"
     CDS             18688..18945
                     /gene="rpsQ"
                     /locus_tag="PLG98_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOE32831.1"
                     /translation="MDQRGFNRTIKGVVVSNKMDKTIVVRAERFVKHPVFHKYVRKHV
                     KYKAHDERNQSKIGDTVLIIESRPLSREKRWRMLEILEKAK"
     gene            18991..19359
                     /gene="rplN"
                     /locus_tag="PLG98_05945"
     CDS             18991..19359
                     /gene="rplN"
                     /locus_tag="PLG98_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOE32832.1"
                     /translation="MIQMQTILNVADNSGAKKVACIKVLGGSKRRYASLGDVIVVSVK
                     EAIPNSKVKKGDVIKAVIVRTVKEVRRPDGSYLKFDDNSAVLISNQMEPVGTRIFGPV
                     ARELRARQFMKIISLAPEVL"
     gene            19382..19696
                     /gene="rplX"
                     /locus_tag="PLG98_05950"
     CDS             19382..19696
                     /gene="rplX"
                     /locus_tag="PLG98_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOE32833.1"
                     /translation="MSLNKFKKGDTVKVLAGKDKGKTGKVLAVIPEKDRIVVEKVNVI
                     KKHKRPDQRSKGGIVEKEGSIHVSNVGLFCNKCNKPVRVQSKKLEDGKKVRICSKCKD
                     VI"
     gene            19712..20251
                     /gene="rplE"
                     /locus_tag="PLG98_05955"
     CDS             19712..20251
                     /gene="rplE"
                     /locus_tag="PLG98_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOE32834.1"
                     /translation="MARLKEYYSKTVAPALVKEFKYKNPMQVPKMEKIVINMGLGEAI
                     QNVKIIDGAVQELAAITGQKPIVNKAKKSIATFKLRQGMPVGCSVTLRKKIMYEFFDR
                     LVNAALPKVRDFRGISPHAFDGRGNFSIGLQEQIIFPEIDYDKIEKNKGMNITIVTTA
                     KTDEEARFLLKMMGVPFKQ"
     gene            20278..20463
                     /locus_tag="PLG98_05960"
     CDS             20278..20463
                     /locus_tag="PLG98_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOE32835.1"
                     /translation="MAKKSLIAKAKREKKFSVREYNRCPLCGRPRAYYRKFDMCRICL
                     RKLSLQGELPGVIKSSW"
     gene            20488..20889
                     /gene="rpsH"
                     /locus_tag="PLG98_05965"
     CDS             20488..20889
                     /gene="rpsH"
                     /locus_tag="PLG98_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOE32836.1"
                     /translation="MGMTDPIADMLTRIRNANKARFKTVRVSMSQVNVNVAGVLKKTG
                     YITNYEKVKDEKGRQMLEIILKYPDAKRTVITNIKRVSKPGRRIYVPADNIPKVLNGL
                     GISILSTSRGVITDAEARELNVGGEILCNVW"
     gene            20925..21464
                     /gene="rplF"
                     /locus_tag="PLG98_05970"
     CDS             20925..21464
                     /gene="rplF"
                     /locus_tag="PLG98_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOE32837.1"
                     /translation="MSRIGKKPVSIPKNVKISQSADTVKVEGPKGALSSRMPEGIAIK
                     ISDGTLSVERKSEALTTRSYHGLTRTLIDNMIKGVTTGFEKRLEISGVGYRAEANDKS
                     LKFILGFSSPVQYDIPKGIDIKIDKQVNIVVSGIDKELVGRVAAEIRALRKPEPYKGK
                     GIKYANEYIKRKAGKSAGA"
     gene            21503..21868
                     /gene="rplR"
                     /locus_tag="PLG98_05975"
     CDS             21503..21868
                     /gene="rplR"
                     /locus_tag="PLG98_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOE32838.1"
                     /translation="MASKKTLKIREKREKRTRSKIKGTQERPRLSVFRSDRHIYVQAV
                     NDVTADTLATASTLCKELESRLKGKKKVDVAKEVGKLIAERLKAKGIEKVVFDRGRFL
                     YHGRVKALAEAARESGLKF"
     gene            21926..22390
                     /gene="rpsE"
                     /locus_tag="PLG98_05980"
     CDS             21926..22390
                     /gene="rpsE"
                     /locus_tag="PLG98_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOE32839.1"
                     /translation="MKDMELLDRVVAINRTAKVVKGGRRFRFSAIVVVGDGKGMIGTG
                     LGKAHEVPEAIRKGMDMAKKNMVRVAVVDRTIPFEVIGRYGAGKVLLKPASEGTGLIA
                     GGAVRAVLEVAGVHNVLTKCLGSHNPHNLVKATFEGLCQLKNPEEIAAQRGK"
     gene            22431..22610
                     /gene="rpmD"
                     /locus_tag="PLG98_05985"
     CDS             22431..22610
                     /gene="rpmD"
                     /locus_tag="PLG98_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOE32840.1"
                     /translation="MLKITKVKSVIGRPEKQRKILKGMGLTKLNKTVTLEDTPQIRGM
                     INKVTHLVSVEESKE"
     gene            22614..23060
                     /gene="rplO"
                     /locus_tag="PLG98_05990"
     CDS             22614..23060
                     /gene="rplO"
                     /locus_tag="PLG98_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOE32841.1"
                     /translation="MDLGSLKAPAGATKKRKRVGRGNASGHGGTSGKGHKGQKARSGG
                     KVRVGFEGGQMPMARRLPKRGFRNPFRKEYIAVNILDLCRKFSKGAVIDEAALLQSGL
                     IKTEGAHIKILAKGDIDFPVTLKIARISPAAKDKIIAAGGSVEEVI"
     gene            23062..24369
                     /gene="secY"
                     /locus_tag="PLG98_05995"
     CDS             23062..24369
                     /gene="secY"
                     /locus_tag="PLG98_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOE32842.1"
                     /translation="MLEGLGSVSKIPELQRRILFTILLLGVYRIGVYIPTPGIDNVAL
                     LAYFEHAKGSLFGLMDMFAGGAFSNFSIFALGIMPYISSSIILQLLTIAVPHLERLSK
                     EGESGRRKITQYTRYGTVLLSMIQGFGIAFGLQQMAGPSGAPIVLNPGIGFVLMTMIT
                     LTSGTAFIMWLGEVITEKGIGNGISLIIFAGIVCRIPAAIGNTFRLATAGELGVLVIL
                     LITVLMIAVVGAIVFVEGGQRRIPVQYAKRVVGRKMYGGQTTHLPLKVNTSGVIPPIF
                     ASSVIMFPATIANFAPQTWMKDIAAYLVPGRLAYEVLYVAFIFFFCFFYTAVTFKPDD
                     VAENMKKYGGYVPGIRPGKKTAEYIENVLEKLTFSGAIYVSIICVLPSVLIAQLNVPF
                     YFGGTALLIVVGVAMDTASQIESHLLTRQYEGFMKKGHIARRR"
     gene            24382..25134
                     /gene="map"
                     /locus_tag="PLG98_06000"
     CDS             24382..25134
                     /gene="map"
                     /locus_tag="PLG98_06000"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOE32843.1"
                     /translation="MVILKLPEEIERARASNRIVAEVLSRLREKVKPGVKTKDLDKFA
                     EEIAEKRGARPAFKGYHGYPHSLCVSINEVVVHGMPSERVLEEGDIVGLDFGVFYQGF
                     FGDATVTLPVGKVTAKALRLMQVTEQSLYAGIAQAREGNRLGDISAAVQSVVEEAGYS
                     VVRDFVGHGIGKDLHEEPQIPNFGQKGRGIELKKGMILAIEPMVNEGKYKVQVLPDGW
                     TVVTKDGSLSAHFEHSIAITEHGPDILSRLNY"
     gene            25156..25374
                     /gene="infA"
                     /locus_tag="PLG98_06005"
     CDS             25156..25374
                     /gene="infA"
                     /locus_tag="PLG98_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOE32844.1"
                     /translation="MAKEEAIEVEGRVIEPLPNAMFRVELDNGHKVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRITYRTK"
     gene            25393..25506
                     /gene="rpmJ"
                     /locus_tag="PLG98_06010"
     CDS             25393..25506
                     /gene="rpmJ"
                     /locus_tag="PLG98_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOE32845.1"
                     /translation="MKVRSSVKKICEKCKIVKRKGVLRVICENPKHKQRQG"
     gene            25532..25900
                     /gene="rpsM"
                     /locus_tag="PLG98_06015"
     CDS             25532..25900
                     /gene="rpsM"
                     /locus_tag="PLG98_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOE32846.1"
                     /translation="MARISGVDLPKNKRMEIALTYIYGIGKTRAKQILKDAGVSPDTR
                     TDDLADSEISAIRGIIDKEGKVEGDLRRDISMSIKRLMDVGTYRGLRHRKGLPVRGQR
                     THTNGRTRKGPRRAIAGKKK"
     gene            25927..26319
                     /gene="rpsK"
                     /locus_tag="PLG98_06020"
     CDS             25927..26319
                     /gene="rpsK"
                     /locus_tag="PLG98_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOE32847.1"
                     /translation="MAKAVRKTGKKKEKKNITDGIAHIQSTFNNTIVTITDMSGNVIA
                     WSSSGLQGFKGSRKSTPFAAQMAAEDVVRKAKEQGMRKVQVYIKGPGAGRESALRSLQ
                     IAGLTITLIRDVTPVPHNGCRPPKRRRV"
     gene            26367..26996
                     /gene="rpsD"
                     /locus_tag="PLG98_06025"
     CDS             26367..26996
                     /gene="rpsD"
                     /locus_tag="PLG98_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOE32848.1"
                     /translation="MARYTDSSCRLCRREGLKLFSKGDRCYSEKCSFERRDYVPGDHG
                     QMHKKQRSDYGVQLREKQKLKRMYGLLEKQFRSYFEKADKQKGITGTNLLILLERRMD
                     NMVFRMGFANSRIESRQLISHGHFLVNGKPVNIPSYLLKAGDEVSVKENSRKVTRIMG
                     ALETVVRRGVPQWLELDKDNFNAKIKTLPVREDLTMPIQEQLIVELYSK"
     gene            27042..>27247
                     /locus_tag="PLG98_06030"
     CDS             27042..>27247
                     /locus_tag="PLG98_06030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOE32849.1"
                     /translation="MQRNWSSLIRPKRIDVDEATHTNFYGEFTIQPLERGFGITLGNA
                     LRRVLLSSIQGSAIVSAKIEGVLH"
BASE COUNT         7833 a         5569 c         7478 g         6367 t
ORIGIN      
        1 agcgccggac tccaaatccg gatgctgggg gttcaagtcc ctcctggcct gccatttttc
       61 cccgttgtgt gaaggatgag tcagatggaa gattttaaag aaaaagtaaa agtaacgatt
      121 caaaagatta cacagtttct caaagatgcc aagatggagt tgaaaaaagt tacctggccg
      181 actccaaaac aggccctggc gtctaccgcg gtggtgatca ttctggtttt tattgtttct
      241 ttaattctgg gcattattga ttttgccctc gctaaagcag tgaaattaat attgggttag
      301 gaaaatggct tttaaatggt acgttgtaca tacttattcc ggatacgaga ataaagtcaa
      361 aataagtctt gaagaaagaa ttaagacgac cggaactcag gcgtattttt ccgacattct
      421 gattcctgaa gaagatgttg tggaattgat ttcgggagag aaaaaaagtt cgaaacgcaa
      481 gtttttcccg gggtatatcc ttgtgcggat ggatatgaat gatgaagcct ggcacgtggt
      541 caaggatact cccaaagtga caggattcat cggcagcaag gataaacctt ctcccatacc
      601 tgataaagac gttgaacatc ttaaaatcag aattgatgaa ggaacgttaa aacccaaacc
      661 caaattcaaa ttcgatgtcg gggatcatgt caaaattatc gacggtcctt ttacgaattt
      721 cgacggcatt atcgatgaat taaaaccgga aaaaagtaag cttagggtta tcgtaagtat
      781 ttttggccgg ccgacgccag tggagctgga tttcatccag gtgacgcagg ggtaatgctt
      841 tgcataggcc gaataaataa tatttaaaat tcagatcagg gtgattcgat atggcaaaaa
      901 aaattattgc gaacattaaa ttacagatca aggcgggcaa ggcaacccct tcgcctccga
      961 tcggtccggc attaggtcag cacggcgtga atatcatgga attctgcaag gcttataacg
     1021 cgatgacgca gagccaggaa ggaatgatta ttcctgttgt gattacggtt tatgccgatc
     1081 gctcttttac gtttattaca aaaacacctc ctgtgtcggt gttactgaag caggcagcaa
     1141 aaattgccaa gggatccagc gatcccaaac gggataaagt gggttcggtg accgcatctc
     1201 aggtaaagga tattgccgaa ctgaagtata aggatttaaa cgccgttaat attgaaggcg
     1261 cgataaatat cattgccggc acggcaaggt caatgggaat agaaattaca ggttaataaa
     1321 taaagaggtt tgatcatgtt aagaagaggc aaacgtatat tggcggcaaa ggctaaagtc
     1381 gagccggcca agaaatacac gttgaaagag gctgcggaaa ttgttgtatc gacggcggga
     1441 acgaaatttg atgagaccgt ggatgcggcg attcgtttgg gtgttaatcc cgctcacgcc
     1501 gatcaaatgg tgcggggcag cgtggttctg ccgcacggat tgggtaaaac agtcagggtc
     1561 ctggtatttg ccaaaggtga aaaggaaaaa gaagctttgg aggccggtgc cgatctggtg
     1621 ggcaatgacg aagttatcga gaaaatcaaa ggcggatgga tggaatttga tcgggttatc
     1681 gccacgccgg atatgatggg atcagtcggt aaaatcggta aaattctcgg acctcgcggt
     1741 ttgatgccca accccaaggt ggggacggta acgtttgacg tggccaatgc ggtgaaagaa
     1801 ctcaaagcgg gtaaagtcga gttccgggtc gaaaaagccg gcattgttca ttctccggtg
     1861 ggaaaggtat ccttcggtgc tgaaaaattg agagaaaatg ttagtgcctt gctggagacc
     1921 attcttaaat taaaaccggc atcaagcaag ggtacgtata tcaaaagcat ttcgatctcc
     1981 agcacgatgg gtgtcggggt gagaattgat ccgctcgata ttaaagcagc ttaggatatc
     2041 gggcattttt tagttggtta caaggtcgaa gacagcaggt acagacgttt aaacctgaaa
     2101 aggcctgccg agactcgtaa aataaaaatt gttacttgat ttgtaagggt agactttttt
     2161 tgtaaaacgg gaatcgggat gtaggctggg ttaccgtttt tttgttgtga gactcaaatt
     2221 tcaaaaacga atgcgctaca gggcttcata ggtgtattga aatttgtaag tcgaacaatc
     2281 ccggtatcgg ggttctgtat gtggcttaat tctccggcct cagcaatata ataattttga
     2341 aaggaggctg atagattgga tcggaaaacg aaagaacaaa ttgtatccga actccaaaag
     2401 aaactcaaag aagctaattt aggtattctc accagcttta gcgggatgaa tgtcgaaaaa
     2461 atggaagctt tgagaaatga attgcgcaag tccaatgccg aatggaaggt cgtaaagaat
     2521 acacttttac gcattgcttc cgaaggcacc gatttcagca tacttgccga tcatttcaaa
     2581 tggcccgttg ccattgtcct gagctacaaa gatccggtgg cgccggcgaa ggttctgatt
     2641 gattttgcca agaaaaacgc ggaactggaa atcaaggttg gtgtgctgga taaaaagctt
     2701 ttaaccagga acgacctgac ggttctggca gaattgcccg gcaaagacgt ccttctgggc
     2761 aaactggttt cggtcatggc ggctgttccg acatccttcg tgagagtttt aaatggagta
     2821 ccgggaagtt tcgtgcaagt gcttaacgct tattgcgata agaaaaaatc tttaaactaa
     2881 tacaacatac agaaaggaat aaaacatgtc agataaaatt acgaaagaag atgtggtcaa
     2941 atttattgaa gaaatgacgg tattggaact ttccgctctg atcaaagagc tggaggataa
     3001 atttggtgtt tccgctgccg ctccggttat ggccgccgtg gctgccccgg gtgccgccgc
     3061 tgctgcaggt ccggttgaag aaaaaacaga attcgatgtc attctgaccg gctttggcgc
     3121 ggagaagatt caggtcatca aggttgtccg tgccattacc aacctgggac tcaaagaagc
     3181 caaggatctg gtggaaggcg ttcccaagcc catcaaggaa ggtgcgtcca aggatgaagc
     3241 tgcggacatc aagaagaaaa tcgaagaagt gggcggtacc gtcgagatta aataacgcaa
     3301 tctcaaacat atattattca tttttaagga tgccatggga gactttagaa aaaattttgg
     3361 tcaggtaaga aagatactgg atatccccaa tttaatcgat attcagacag agtcttacga
     3421 gaaatttctc cagaaggata ttgcaccggt gaagcggggc gctttcggtt tgcagggtgc
     3481 tttcaagagc gtttttccta tttcggattt cagcgggaaa tgttctctgg aatttgtgag
     3541 ctataaactg ggtgatatcc ggtatgatgt gaaagaatgt gttcaaaaag gaatgaccta
     3601 cgcggctccc ttgaagatcg tggtccggtt ggttgtgttt gaggccgatc gccccgccga
     3661 agggaaaggt gccggtgaaa cgaccgaagc caagcagatc cgcgatatca aggaacagga
     3721 agtttacttc ggcgaaattc cgttgatgac ggaaaacggt accttcatca ttaacgggac
     3781 ggaacgggtg atcgtcaatc agctgcaccg ttcgccgggt atcttttttg atcacgacaa
     3841 aggtaaaatt gttgccagcg gtaaactgat ttattccgca agagtcatcc ccatcagagg
     3901 atcgtggctg gatctggagt ttgattccaa agatattctt tatgtccgga tcgaccgtcg
     3961 cagaaaaatg ccggtaacca tcctgctgaa agcgctgggg aattcgacgg aattcatcct
     4021 gaattatttt tataacgtca acacagtgat caatgacaat aaaaagattt attttgccgt
     4081 tgatgaatcg ctggtcggca agaaatcacc agaagatatc aaagactcca agagttccga
     4141 actgattgtc aagaagggaa gaaaactgac caagccgctg atcaagagaa tcatggaggc
     4201 cggtgttaaa cggacggcaa tcaatgaaaa cgaactgatc ggaaaaattc cttctcggga
     4261 tgttcatgat ccggaaacgg gtgagattgt ttttcgttgc aacgaggaac tgccggcgaa
     4321 ctgtctggac attatccgcg aaaagaatat ccgggaattg aaacttattc atattgatga
     4381 agacatggat aacgcttcca tccgggatac gctgctgctg gataagattg ataccaccga
     4441 ggatgcgatc atggagatct atcgccgttt gcgtccgagc aatcctccta cgccggaaac
     4501 agcggacaag ttcttcaaga gcctgttctt tgagccggag acttatgatc tgtccaatgt
     4561 cggacgcgcg aaaatgaatt acaaattgca catgaacgtg ccgacggaca acaccgtgtt
     4621 gagcaacgaa gacatcctgt ccgcagtgaa atatctgatc gacctgaaaa acggcgtcgg
     4681 cgattgcagt gtggacgata ttgatcacct gggaaaccgc cgggtgcgtt cggtaggcga
     4741 attgattgaa aaccagtacc gcatcggtct ggtgcgtatg gaaagagcca tcaaggaaaa
     4801 aatgagtctt caggacatcg agaccatgat gccgcacgac ctgatcaatg ccaaaccggt
     4861 ttctgcggtg gtcaatgaat tcttcaattc cagccagttg tctcagttca tggatcagac
     4921 caatccgctt tcggagatta cccataagcg acgtctgtcg gctctgggtc caggaggctt
     4981 gacccgtgaa cgcgcgggat ttgaagtccg cgatgttcat ccgacacatt atggacgcat
     5041 ttgtccgatt gaaacaccgg aaggtccgaa catcggattg atcgtgtcgt tgagcaccta
     5101 tgccagagtg aatgaattcg gttttattga gacaccgtac cggaaagtgg agaacggccg
     5161 ggtgtccgat gatgttcact ttgtaacggc gattgaggaa gaaaatcaga ttattgcttc
     5221 ggcggaccgt cctctgagta aaaacggaaa atttgccgaa gaattgattt cggtcagaaa
     5281 aggcggcagc tatatttcgg ttgagccgga agagattaag atgatggacg tttcgcccac
     5341 ccagctggtc agcgtagctg cagcgttgat tccttttctg gaacatgatg acgccaaccg
     5401 tgcgttgatg ggatcgaata tgcaacggca ggcggttccc ctgatgaatc cggaagttcc
     5461 ggttgtgggg acaggcgtgg aatccgtggt agcccgcgat tccggcgctg tggtcctggc
     5521 caaacggtcc ggtgttgtcg aaagcgttga tgcttcacgc attattgtgc gggccgaccg
     5581 gatggatgaa gacggcatcg acagcggtgt ggatatttat aatctggcga aataccagcg
     5641 atcgaatcag gatacctgtt tcaatcaaaa accgatcatc agcgtcgggc agcgtgtcca
     5701 taaaaaggat atcctcgcgg acggaccggc cattgataac ggcgaactgg cactgggtag
     5761 aaatgtaatg gtcgctttca tgtcctgggg cgggtacaac tatgaagact ccattctggt
     5821 cagcgagagg gtcgtcaagg aagatatttt cacgtcgatt catatcgagg aattcgagac
     5881 catgtcccgc gacacgaaac tgggcaaaga ggaaattacg cgcgatatcc ccaacgtcgg
     5941 cgaagaggtt ctcaaaaacc tggatgaaag cggtattgtt tgcattggcg tttcggtcaa
     6001 acctggggat attctggtcg gcaaaataac gcccaagggc gaaacacagc tgtccgctga
     6061 ggaaaaactt ctgcgggcga ttttcggaga gaaagccagc gatgtccggg atacatcgtt
     6121 gcgcgttccg ccgggcgtgg aaggaacggt tattgacgcc aaaattttta ccagaaaagg
     6181 cgcggaaagc gattcccgtt cacaggccat tgaggcggat gccattgagc agatcgcgaa
     6241 ggacagagat gatgaaatcc gcattctcac cgaaaccatc aaaaaagata ttaccaaact
     6301 tgttgtcggc aaaacggcgg caaccagaat tggggatgcc aaaaagaaaa ccgttctgaa
     6361 gaagggcgac aaaattacga aagagatctg gaacgatctg cctttctcca gctggaaaga
     6421 tattaccatc gccgagaacg acggttcgga agaaaagatc gccggtctga tcgcttcact
     6481 ggaacgaaag atcgattttg tcagtgctca ttttgaggag aagatggaaa agatcaaggc
     6541 cagtgatgaa cttccgcccg gtgtaatcaa gatggtcaag gtgtatattg ctattaaaag
     6601 aaggctgtcc gtgggcgaca agatggcagg gcgccatggg aacaaaggcg ttttatcacg
     6661 gatcctgccg gaagaagaca tgccgtattt tgccgacggt tccacagtgg acatcgtctt
     6721 aaatccgctg ggtgttcctt cgcgaatgaa cgtggggcag attctggaaa cgcatctggg
     6781 ctgggcggca aaatcgatcg gtgagggttt gaatgaactg ctggaacagc agcgcgccgg
     6841 tttgagtcat atcaaagacg agttgaaaaa ggtttactct tcgcccgatt ttgacaaatt
     6901 tatcgatcag gcgtccacgg aagatatcaa acgatttgtc ggccgtttga agaaaggcat
     6961 gttggttgcc agtccggttt ttgacggatg tccggaagat caaatcagag agatgttgaa
     7021 aagaaccgat ttgccggaga ccggccagtc cattcttttt gacggcagaa ccggcgagcc
     7081 ctttgatcag gaggtgacgg taggcattat ttacatgatg aagctgcacc acctggtgga
     7141 caacaagatc cacgcgcgct ccatcgggcc ctattcgctg gtgacccagc aaccgctggg
     7201 cggcaaggcg cagttcggcg gccagcgact gggcgaaatg gaagtctggg ccatggaggc
     7261 ttatggcgcc gcatatgctt tacaggaatt tctgaccgtc aaatcggatg atgtggcggg
     7321 cagaacaaga atatacgaat ccattgtcaa gggtgaacat aattttgagt cgggcctgcc
     7381 ggagtccttc aacgttttgg tgaaggagct tcaggggctt ggattggatg tggaattagt
     7441 cgaagagtcg gatagcctaa gttagcctag gggtatcgcg gcaaaaaaag caacaacagg
     7501 cggcttaata atttcacacc gttatttacg tacaggagaa aatttgtgga agacgttttc
     7561 agctattttg agaaaccgaa gaatttggtt aaattcaacg caatgcagat ttccattgct
     7621 tcaccggata aaattctgtc ctggtcccat ggtgaggtga aaaaaccgga aaccatcaat
     7681 taccgaacat ttaagccgga aaaagacggt ctcttctgcg cgaagatttt cggtccggtc
     7741 aaggattacg aatgtctttg cggacgttat aagagaatga agcaccgcgg cgtggtctgt
     7801 gaaaagtgcg gggttgaggt cattcagtcc aaagttcgcc gcgagcgcat gggccacata
     7861 acgctcgcga cgcccgtcgc tcatatctgg tttttgaaaa gtcttcccag ccgcatcggc
     7921 aacgcggtgg acctttctct taaagaattg gagaaagttc tttactttga atcatggatt
     7981 gttcttgacc ccaagaatac gccattgaag aaaaaagatc tgctttcgga tgaggaatac
     8041 gaagaggcca gggaacagta cggtaatgac ggttttgtcg ccggtatcgg cgccgaggcc
     8101 atcagacaac tgctggaaga aattgatctg gaaaaactct acgaagaact gcgtacggaa
     8161 atcgtgacct ccgtttccga taccaagaaa aagaagctgg tgaagcgctt aaaggttgtt
     8221 gaagcgctgc gcaaatccgg caataagccc gaatggatga ttctcacggt gctgccggtg
     8281 attccgcccg atctgcggcc gctggttcca ctggacggcg gacgttttgc cacatcggat
     8341 ctcaacgatt tatatcgccg cgtcattaac cggaataatc gtctgaagag attgcaggaa
     8401 ctcaacgctc ccgaaatcat cattcgcaat gaaaagagaa tgctgcagga agcggtggac
     8461 gttctttttg acaacggccg acgcggccgt gcgattaccg gaaccaacaa aaggccgttg
     8521 cgctccctgt ccgacatgct caaaggcaag cagggccgtt ttcgtcagaa cctgctgggc
     8581 aagcgcgtcg actactccgg acgttccgtg gttacggtcg gcccggatct gagactgcat
     8641 cagtgcggtt tgccgaagaa aatggccatc gagcttttca agccttttat ctacaatcgg
     8701 ttactggaaa aaggatatgt aaccacggtg aaaagtgcca aaaaaatggt ggaaaaggaa
     8761 tcggcggaag tctgggatgc gctggatgaa gtcgtgcgcg agtatccgat catcttaaac
     8821 cgcgcgccga cgctgcatcg tctgggcatc caggcctttg aaccggtcct gatcgaaggc
     8881 aaggccatcc agcttcaccc gttggtttgt acggcgttca acgcggattt tgacggcgac
     8941 cagatggctg tgcatattcc gctgtccgtt gaagcacaga cggaagcacg cgttctgatg
     9001 atgtccacca ataatattct ttctccggcc aacggcagcc cgatcatcat tcccagccag
     9061 gacatcgttc tgggtattta ttacctgacc cgggccaaga ccggcgtcaa gggcgacggg
     9121 atgatttttg cgggtcccga cgaagtgcgc gccgccattg ataacggcgt gattgacctg
     9181 catgcgcgga tcaaggtgcg cattgattcg gaactgaaag aaaccacggc tggacgaatc
     9241 attctttccg aaatcgtgcc cgagcagatc agcttcgacg atatcaacaa actgatgaac
     9301 aagaaagaac tgaccagcct gatagatcat tgttatcgtc tgtgcggcga caagacaacc
     9361 atcattctgg ccgataaact caaggatctg gggttcaagt atgcgaccgt ttcggggctc
     9421 tcctttgccg tgggcaatat gatcattccg gagcaaaaga aaaaaattgt cctgcaggcc
     9481 gataaagatg ttttgaagat tcaaagacag tacatggacg gcttgattac cgacggagaa
     9541 cgttacaaca aagttgtgga catttgggca caggccactg aaaaaatagc atccgaaatg
     9601 ctcaaaggca tcagtaccga agatgtcccg gccgccgagg gaaagaaacg caaaatcgag
     9661 agtttcaatc ccatttacat gatggctgat tcgggtgccc gcggctccgg acagcagtta
     9721 agacagctgg ccggtatgcg cggtctgatg gcgaagccct ccggtgaaat tatcgaaacg
     9781 cctattacag cgaattttcg cgagggattg acggttcttc agtactttat ttcaacgcac
     9841 ggcgcccgta agggattggc cgatacggcg ctgaagacgg ccaactctgg ttacctgaca
     9901 cgaagactcg tagatgtggc gcaggactgc atcatcacgg agcatgactg cgaaacagtg
     9961 gatggtgtgt atgtatcagc tctgatggaa ggcggcgaaa tcattgaaac cgccggagaa
    10021 cgtgttctgg gaagagttgc gctgcaggat gttaaagatc cgtttagcgg agaagttatc
    10081 gtgccggcca atcagatgat tgatgaaacg ctggctgaaa agatcgacaa tgccggtatc
    10141 gaaaaagtcc atattcggtc ggttctgacc tgtcgttcca agcacggggt ttgtgcgatg
    10201 tgttatggac gtgacctggc gcacggccac ctcgttaata tcggtgaagc ggtcggtatt
    10261 gttgccgctc agtccatcgg cgaaccgggg acccagttga ccatgagaac gtttcatatc
    10321 ggcggtacgg caaaatttga cgaacattcg actctggaag cccgtcacga cggaattgtg
    10381 aaatttgtaa atctcagcac ggtaaaaaca aaggaagggg acctggtcgt gatgaaacgc
    10441 aacggcgaag tccacgtgct ggatgaacag aaacacgttc gcggcaagta cgtcctgtcg
    10501 tacggcgccc acctgaaggt tacggataac agcgaagtga aaaaaggaca gttgattgcc
    10561 gaatgggatc ccttctccat accgattctg gcggaagtgg acggaacgat caagtttgat
    10621 gatatcaccg aaggaaagac catgcaggaa caggttgatg aagtaaccgg tctttcacga
    10681 aagatcatcg ttgagtttaa gggcggggat ttgagacccc gtgtttccat taaggacgaa
    10741 aaaggaaaaa cagccaaact ggcggattcc aatgccgttg cacggtatct gctctcagta
    10801 ggggtcaacc ttgtggtttc tgaaggagat caggtgagag ccggtgatat tatagcgaaa
    10861 attccccggg aaacgacaaa aacgaaagat atcacgggtg gtttgccccg cgttgccgaa
    10921 ctgttcgagg cgcgtaaacc caaagatttc gccgtgatca gtgaaatcag cggtgtggtt
    10981 tcctacggga aagatgccaa agggaaacgt aagatcgttg ttaccccgga agtgggcgag
    11041 gaaaaagagt atcttattcc caaagggaaa catatcagcg ttcaggaggg cgatgttgtt
    11101 tctcccggtg atgccctgat gtccggtgtc aacaatcctc acgatctgct gtccattaag
    11161 ggcgaaaaag aactggcccg ttatctggtg gatgaagttc aggaagtcta ccgtttgcag
    11221 ggcgtgaaaa tcaatgataa acacatggaa gtcattgttc gccagatgct cagacgcgtt
    11281 aaggtaatgg atccgggaga taccgcattt atcgccgacg aaaaaatcga acgataccgc
    11341 ttccaggaag aaaacgaaaa gattattgcc cagggcggcc gacccgctat cggcgagccg
    11401 atgcttctgg ggattaccaa agcatcgctt tccacccaga gctttatttc ggcagcgtcc
    11461 ttccaggaga ccacaaaggt cctgacggaa gccgcccttt ccggtaaaac ggattattta
    11521 agggggctta aggaaaacgt cattatgggc cggttgatta ccgccggaac cggtttggtc
    11581 atgtaccgga agttgggaat tgcgctggaa aatgagaatg ccgagacggc ggcagaataa
    11641 attaaaatat cggcagaaaa tgcttgacat tagtaatttg aactggtacg taccaccggt
    11701 tcgctgccgg ttataataca ttgaagagca gcgatttgtt ttaggaggaa aatgccgaca
    11761 attagtcaac tggttcgcaa gggcagagca aagattaaga gaaaaacaaa ttcacccgcg
    11821 ctggcgggtt cgccgcaacg tcgcggtgtt tgtgttagag tgtatacaac gacaccgaag
    11881 aagccgaatt cggctttaag gaaagtggcc agagtgcgat tgaccagcgg ttatgaagtt
    11941 acgtcataca ttccgggtat cggtcataat ctgcaggagc attcagtggt tctggtccgc
    12001 gggggaaggg tcaaggatct gcccggtgtc cggtatcatg tcatcagagg aactctggac
    12061 acggtcggcg ttcaggatcg caaacagggt cgatcgaagt acggtgctaa aaagccaagt
    12121 taaacggaga ggattcaatg ccgagaagac gggaagttga aaagagagat attttgcccg
    12181 atcccaagtt taataataaa ctggtcgcaa aatttattaa ttcgctgttg aaaagaggta
    12241 agaagagcgt tgcagaaagc attctctacg gcgcttttga tattattgaa aagcgggtca
    12301 aggaacaacc ggtggatttt tttgaaaagg ctgtcaacaa tgtgaaaccg gtgatcgaag
    12361 tcaaatccag gcgtgtcggc ggttctacct atcaggtgcc gacggaagtt gttccggcaa
    12421 gacgcaccgc gctggcgatt cgctggttga tatccaatgc ccaggaacgt acggaaaaaa
    12481 ccatgcggga aaaactggct tcggaattga tcgatgccgc caacaaccgc gggggagcga
    12541 ttaagaagaa ggaagctgtg cataaaatgg ccgaggcgaa caaagccttt gctcattata
    12601 aattttaaat cataatttta acgataagga aaccaaccat tcaaagttaa ggaggaaagg
    12661 tgaaatggca aagaaaaaat ttgaaaggac taagccgcat ttaaatatag gaacgatcgg
    12721 tcacgtggat catggtaaaa ccacattgac ggccgcgatt accaaatatc tggcaaaaaa
    12781 gggttttgca gaattcagag cttttgattc catcgataat gctccggaag aaaaggaacg
    12841 cggcgttacc atcaatattt cccacgtgga atatgagacg gaaaaaagac attacgctca
    12901 tgtggattgc cccggccatg ccgactatat caaaaatatg atttccggcg ccgcccatat
    12961 ggatgggacc attctggtag tggcggcttc cgatggtccg atgcctcaga cacgcgagca
    13021 tatcctcctt gctcgccagg tgcaggttcc ttacattgtc gtttttctga ataaagtgga
    13081 tctggttgat gatcccgagc tgttggacct tgtcgaactt gaattgcgtg aacttttgac
    13141 ccagtatgaa tttcccggtg acaatattcc tatcattcgc ggttcggcac tgaaagcctt
    13201 ggaggccgac gatatcaatt cccctgatgt gaaatgcatt gcggaactga tggatgccgt
    13261 ggataattat gtaccggaac cgaagcgcga tacggacaaa ccgtttctga tgccggtcgg
    13321 tgacgttttc accatttccg gccgcggtac ggttgtgacc ggaagagtag accgcggtat
    13381 tgtcaaggtc ggtgaagaag tggaaatcat cggaatcaga gagaccatta aaacggttgt
    13441 gaccggtgtg gaaatgtttc gcaaaacgct ggatgaaggt cgtgccggtg acgacgtcgg
    13501 cttgttgctc cgcggaatca agcgggaaga agttgaacga ggacaggttg tcgccaagcc
    13561 cgcctccatt acaccgcata ccaaatttga agcggctgtc tatgttctga ccaaggaaga
    13621 aggcggtcgt cataccccgt tcttctccgg ttatcgtccc cagttctact tccggacgac
    13681 ggacgtgacg ggtgttgcaa cgcttcccga gggcgtggaa atggtgatgc ccggcgacaa
    13741 cgtcaaaatg tcaattgaat taattacccc cattgcaatg gaagaacagc tgagattcgc
    13801 tattcgtgaa ggcggcagaa ccgtaggcgc cggagtcgta agcaaggtta ttgcatagaa
    13861 aataggttag ctaacccatg aaagaacaaa agattagaat tcggcttaaa gcttacgatt
    13921 ataaactgtt ggatcgttcc gtcgaggaaa ttgtggaaac ggcgaaaaag accggcgcac
    13981 gtgtcgccgg tcctattcct attcctacgg aaatcaataa gtactgcgtg aatcgctcac
    14041 ctcatgtgga caaaaaatct cgcgagcagt ttgaaatcag aacccacaaa agattggttg
    14101 atatcgtcgg gcctactcaa gccaccgttg acgctttaat gaaactggat ctttcagccg
    14161 gggtggatgt ggaaataaaa gcgtaggcgt gcttttttaa aatgatgaaa aatggcatag
    14221 ggtaatgccg tctttcaaat ggaatatatt attacttaaa aattggaatg tcagatgagc
    14281 aagggaatta ttggtaaaaa actgggaatg acccaagtat ttgcggatga cggatctgcc
    14341 gtcggtgtaa ccgccatcga ggtggaaccc tctgtggttt tacaggtgaa gaccaaagac
    14401 aaggatggct atgatgccgt ccaattgggt tacggccgtg tgaaacagaa aaatgtgacc
    14461 aggccgctgc aaggtcattt taataaggcc aacaagggat ttttccgttt tgtcagagag
    14521 ttcccggcgc attccgaaac gtacgaagtg gggcaggaaa tttctgcgga catatttgcc
    14581 atgggggatt atgtcgacgt tgtcggaaca tcaaagggga aaggttttca gggcgttgtg
    14641 aaaagacacg gtttcggtgg cggcagggca acgcatggat ccatgtttca tcgtgcacca
    14701 ggctcgattg gtgccagtgc tgatccctcc cgtgttttca aaggaaccaa gatgggggga
    14761 cacatgggga atgtccgtaa aaccgttcag aatctcaagg tatggcaggt tcgttccgaa
    14821 aggaatctgc ttttgctgga ggggtccgtt ccgggaagta gaaacagttt tttactgatt
    14881 acaaaggcaa agaaaaaata gcgattacgc taaggctgga gttacgcaca tggcagttgc
    14941 agatgtttta aatatcgaaa aaaagaaagt tgccgaggtg gatttgaatg atgccgtctt
    15001 cggggccgac gtcaatgagg cggtaatata cgacgtcgtc aaaatgcagt tggcctcacg
    15061 acgctccggt acggcctcga cgaaaaccag gagtgacgtg agcggcggcg gtaaaaagcc
    15121 gtttcgccag aaaggaaccg gccgtgcaag ggcaggatcg tcccggtcgc ccgtctggag
    15181 aacaggcgga accgttttcg ggccgcatcc gcgggattat tccttcagcg ttcccaaaaa
    15241 aattcgcaag aaagcactga tctcggcgtt gagcctgaaa ttcaaagaaa ataaaatgat
    15301 catcctgaaa gatttcccga tggagaagat cagcgcgcgc ggatttcaga aagtcgtcga
    15361 tcttttcgat ctcaaaaaag cgttatttgt gctggatgat aacaatgaga ttttgatgaa
    15421 atcttcccga aatattaaaa atgtcaagat gatcagatca gagggtatta atgtttatga
    15481 tgtgttgaat catgaacact tgattctgct tgaaccctcc gtaaaaaaga ttgagggggc
    15541 attgctggta taatggacag aagcaaagtc gtgaagagaa tattggttac cgaaaaaagc
    15601 accatggcgc gcgatgcatc caataaatac ttttttgaag tagatcgcaa agccaacaaa
    15661 gtggaaattg ccgatgcggt tgaaaagctt ttcaaggtca aagtaatgga tgtgcgcgtg
    15721 atgcatgttc tgggaaaaaa gaagagaatg ggaaggacag tcggtcaaaa gagctcctgg
    15781 aaaaaggccg tagtgacatt agccgccggc aacagcattg aatttgctga aggcgtataa
    15841 gaaagaatgc attgaaggat tagacggatg gcaattataa agtataaacc gacatcaccg
    15901 ggtagacgat ttcagagcgt ttccgatttt gccgaaatta cctctacgga gccggtgaaa
    15961 aggcttttaa aacctttgaa gaagaaaggc ggccgtaaca gttacggaag agtcacagcc
    16021 cggcatatcg gcggcggtca caaacggaaa taccgggtga ttgatttttg ccgcaataaa
    16081 gccgacattc cggccaaggt ggcttcgatc gaatatgatc caaacagaac ggctcgaatc
    16141 gccctgctca attatcgtga cggtgaaaaa agatacattg tcgcacccgc gcaggtcaac
    16201 gtcggcgatg tcctgtacag cggtgaaaaa gcggatatca aaccgggcaa cgccgtgccc
    16261 ttgaagtata ttccgctggg gtccttgatt cataacgtgg aattgaaagt cggacggggc
    16321 ggccaactga tcagatctgc aggggcctat ggacagctga tggccaaaga gggaagttac
    16381 gctcaggtac gactgccgtc cggtgaagtc cggaaaatct ttattgaatg tatggcgacc
    16441 atcggacagg tcgggaataa cgaacatgaa aacatcagca tcggcaaagc cggaaggaca
    16501 cgctggctgg gtaaaaaacc tcatgtgcgc ggcgtggtca tgaatccggt ggatcatccg
    16561 atgggcggcg gcgaaggcaa atcctccggc gggcgtcatc cgtgcacgcc ctggggtatt
    16621 cccacaaaag gccataagac cagaaagaac aagagaaccg ataagtatat tattaagaga
    16681 cgaggtaaat aagcgtggcg cgttcgatca aaaaaggtcc gtatattgaa gaaaagttac
    16741 tggctaaaat cagaaaaatg gaggccgaag gaaccaagaa tgtcatcaag acttggtcgc
    16801 ggcgttccac tattacaccg gaactgatcg gttatacttt tgccgtacat aatggaaaaa
    16861 aattcatccc ggtttatgtc acggagaata tggtggggca taaactcgga gagtttgcac
    16921 cgaccagggt tttcttttct cattccggtg atcggaaaac caaagtcagc tagtattttc
    16981 gggagataaa gcggtaaagc ttggagttga taaatatgga agcaaaggct gttgcaaaat
    17041 atatgagaat gtcgccgcag aaagttcggc ttgtggcgga tttgatccgg gggaaaaacg
    17101 ttcaggaagc gcgtaacatt cttctctaca ccaggaaata tgcggcaggt gtcgtggcca
    17161 acgtgctgaa atcagctgtg gccaatgctg cccagaatcc caatattgat gaaaatattc
    17221 tttacgtcaa agaaatttat gtcgatcaag ggcccgcctt gaaacgctgg cgcgccagag
    17281 cgcagggaag agcggcaggc atcaagaaac gaagttcaca tattaccgtt attttggacg
    17341 aaatttaagg aggttcagct tggggcagaa ggttaatccg gtaggattac ggttgggcta
    17401 catcaaaaaa tggcaatcgg tctggtatac cgataaaaaa tacagcgacg tgctgcatga
    17461 agatctgcag atcagaaaat atctgaaaaa gaaactgtat catgcgggtc tatccaaaat
    17521 tgaaattgag agagcagcca acaaagcgaa agtcaatatt tactcggcgc gacccggagt
    17581 gatcattggg aaaaaaggat cggagatcga gaagctcaaa tcagaaattg aaagattttc
    17641 gcagtcggaa attatcatta atattctcga agtccgcaag ccggaagtcg atgcgcaact
    17701 ggtggcggaa aacgtggcca tgcaactgga gaaaagggtt gctttccgcc gggcaatgaa
    17761 gaagtgcgtc gggtatgccc tgaagtttgg cgccaagggc atcaaaatcg cctgttcggg
    17821 aagactgggc ggcgcggaaa tgtcgcgatc ggaatggtac cgcgaaggcc gggtgccgct
    17881 gcatacgctg cgggcggata ttgattacgg atttacggaa gccaaaacgg cttacggatt
    17941 gatcggcgta aaagttttta ttttccacgg agaagtttta ccgacgaaga atgaaaaata
    18001 aattgatgtc catcctgggc gctgaacggt ttgtgcggac agggttggat gttgtcagaa
    18061 acaacgggag tttgttttta tgttaatgcc gaaaagggta aaatacagaa agttgcagaa
    18121 aggccggatg ggcggcaagg cgacaagggg cagttccatc gcttttggcg aatacggatt
    18181 gcaggcggcg gagtgcggtt ggattacgtc ccgtcaaatt gaggctgctc gtgttgccat
    18241 gactcgtcac gtcaaacgtg gcggcagaat ctggatcaga gtatttcctc ataagtccgt
    18301 aacaaaaaag cccgctgaaa cccgtatggg caaggggaaa ggcgccccgg aaggatgggt
    18361 ggccgtcgtg aaacccggtt ccgttctcta tgaaatggaa ggcgtttcga aagatgtggc
    18421 ccgcgaggct tttcggctgg cagcccataa attatccatc gcaacaaaat ttttatcgag
    18481 ggatatagtt gatgaaaatt aaggaaataa gagctcttga ctcaaacggg ctcaaacaaa
    18541 aaatcgctga attgagtgaa gaactttttc ggttgaagat ccggcatgca tcggggcaat
    18601 tggaatcaac cgtcatgctg agcaaaatcc ggaaggatat tgcccgtgtc aacacggtgt
    18661 tgaaagaaaa ggaggcagcg agttaacgtg gatcaaagag gatttaatcg caccattaag
    18721 ggcgtggtcg tcagcaataa aatggataaa acaattgtgg tgagagcgga acgttttgta
    18781 aagcatcctg tttttcacaa gtatgtacgt aaacatgtca agtacaaggc gcatgacgag
    18841 cgcaaccaga gcaaaatcgg agatacggtt ttaattattg agtccaggcc gttgagcaga
    18901 gaaaaacgct ggcgaatgct ggaaattttg gagaaagcca aatagattgt ttgacctgat
    18961 ggaataagtc agtttgatga ggatttttat atgattcaga tgcagaccat attaaatgtt
    19021 gcggataatt ccggcgccaa gaaagttgcc tgtatcaagg tgctgggagg atcgaaacgg
    19081 cggtatgcga gtttgggaga cgtgattgtg gtttccgtca aggaagcgat tcccaattcc
    19141 aaagtgaaaa aaggtgatgt gatcaaggcc gttattgtcc gcacggttaa ggaagtgaga
    19201 agaccggacg gctcttattt gaaattcgat gataattcgg cggttttaat ctccaatcag
    19261 atggaacccg tcgggacgcg catttttgga cctgtcgccc gagaattgcg agccagacaa
    19321 tttatgaaaa ttatatctct agctcctgaa gttttgtaaa aatgttttgc agaggggaaa
    19381 aatgagttta aacaaattta aaaaagggga tacggttaag gtccttgccg gtaaagataa
    19441 agggaagacg ggaaaagttc tggccgtcat tcccgagaaa gacaggatcg tggtcgagaa
    19501 agtgaacgtc atcaagaaac ataaaagacc ggaccagcgc tccaaaggcg gtatcgtgga
    19561 aaaagaagga tccattcacg tatccaatgt cggattgttt tgcaataaat gcaataagcc
    19621 cgtcagagtc caaagtaaaa aacttgaaga tggaaagaaa gttcgtatct gcagtaaatg
    19681 caaagacgta atttaatgtg ggttaaataa aatggcaaga ttaaaagaat attattcaaa
    19741 gaccgtagcg cctgcgctgg tgaaggaatt caagtacaag aatcctatgc aggtgcccaa
    19801 gatggagaaa atcgtcatta acatggggtt gggcgaagct attcagaacg tcaagattat
    19861 tgacggtgcc gtacaggaac tggcggccat aaccggccag aaaccgattg tcaacaaagc
    19921 gaagaaatcc attgcgacct tcaagctcag gcaaggcatg ccggtcggct gttccgttac
    19981 gcttcgaaaa aagatcatgt atgaattctt cgatcgtctg gtgaacgctg ctttgccaaa
    20041 ggttcgggat ttccgcggca tatcacccca tgcgtttgac ggcaggggaa atttttccat
    20101 tggcctgcag gaacagatta ttttccccga aattgactat gataaaatcg agaaaaacaa
    20161 aggaatgaat atcaccatcg tgacgacggc gaaaaccgac gaggaagcca ggtttttgct
    20221 gaaaatgatg ggcgttcctt ttaaacagta aattgtgaag ctccggagga attttgcgtg
    20281 gctaaaaagt cattgatcgc gaaagcgaag cgagagaaga aattttcagt cagagaatac
    20341 aatcgatgcc ctctttgcgg aaggccgcgg gcgtattaca gaaaatttga tatgtgcaga
    20401 atttgtttgc gtaaactttc cctgcaggga gaactccccg gggttattaa atccagttgg
    20461 taactttagc ttaaggagaa aaaaagcatg gggatgacgg atcctattgc cgatatgctg
    20521 accagaataa gaaacgccaa taaggcgcgg ttcaagaccg tgcgtgtcag catgtctcag
    20581 gtgaatgtga atgtggccgg tgtattgaaa aaaacaggtt acatcacgaa ttacgaaaag
    20641 gtcaaagatg aaaaaggccg gcagatgctg gaaataattc ttaaatatcc cgatgccaag
    20701 cgtacagtga ttacgaatat taaaagagtc agcaaaccgg gacgcagaat ttatgtgcct
    20761 gcggacaata ttcccaaggt tctcaacggg ttgggaattt ccattctttc gacgtcccgg
    20821 ggggttatca ccgacgcgga agccagagaa ttgaatgtcg gcggggaaat cctctgcaac
    20881 gtttggtaat ttgaacatca agatattagc aggagctgtg gttcatgtcg agaataggta
    20941 aaaaaccagt aagtattccc aagaatgtta aaatcagcca gagtgctgat acggtaaagg
    21001 tcgaggggcc caaaggggcg ctttcttcca gaatgccgga aggaattgcc atcaaaatca
    21061 gcgacggcac actgtcggtt gaaagaaaat cggaagctct gacgacccgt tcctaccacg
    21121 gtttgaccag aacgttgatt gataacatga tcaagggggt taccaccggg tttgaaaagc
    21181 ggctggaaat atctggagtc ggttatcgcg ctgaagcaaa cgataaaagt ttgaaattta
    21241 ttttaggttt ctcgtctccg gttcagtacg atattcccaa gggaatagat atcaaaattg
    21301 ataaacaggt caatatcgtc gtttccggta ttgataaaga gctggtcggc agggttgcgg
    21361 cggaaatcag ggctttgaga aaaccggaac cgtacaaagg caagggcatt aaatatgcga
    21421 atgagtatat caaacgcaaa gccggtaagt ctgccggtgc ttaattggta aaaaaattat
    21481 attgccgtaa agaggtagga aattggcttc gaaaaagaca ttaaagatca gagaaaaaag
    21541 agaaaaaaga acaagatcaa agattaaagg aactcaggag agaccccgtc tttccgtatt
    21601 ccggagtgac aggcatattt atgtgcaggc cgttaatgat gtaacggcag acacgttggc
    21661 aacggcctcg acgctctgca aggaactgga aagcaggctg aaaggcaaaa agaaggtgga
    21721 tgtggccaaa gaagtgggca aactcattgc cgaacgttta aaagctaaag gcattgagaa
    21781 ggtggttttt gatcggggaa gatttctcta tcatgggcgc gtcaaggcat tggccgaagc
    21841 cgccagggaa agcggtctga aattttaatt tattaatcaa tcaagcgaat aaaggaaaga
    21901 atctttggac aagaaagaaa aagaaatgaa agacatggag ctccttgacc gggttgtcgc
    21961 cattaacaga acggcaaaag tggttaaggg cggaaggcgt ttccgtttca gcgccattgt
    22021 tgttgtcggg gacggcaaag gaatgattgg gaccggattg ggaaaagctc atgaagtgcc
    22081 tgaagcgatt cgcaagggca tggatatggc caagaaaaat atggtcaggg tggccgttgt
    22141 ggacagaacc atcccttttg aagtcatcgg ccgctatggt gcggggaagg ttttgctgaa
    22201 gccggcatct gaaggaaccg gattgattgc cggcggcgct gtgcgggccg tgctggaagt
    22261 tgccggtgtg cacaatgttt tgaccaaatg cctgggatct cataatccgc ataacttggt
    22321 aaaagccact ttcgaaggcc tttgtcagtt gaaaaaccct gaagaaattg ccgcacagag
    22381 aggcaaatag ctctgtcaat tatacggtga ggtagcgtat cgtgggtaaa atgttaaaaa
    22441 taacaaaagt gaaaagtgta atcggccgac ccgaaaagca gagaaaaatt ttaaagggca
    22501 tggggctgac caaattaaat aaaaccgtaa ccttggagga tacaccgcag attcgcggga
    22561 tgatcaataa ggttacgcat ttagtttccg tggaagagtc aaaggagtag aatatggatt
    22621 tgggttcgtt gaaggctcct gccggcgcga caaaaaaacg caaacgcgtg gggcggggaa
    22681 atgcatccgg tcacggtgga acatccggca aaggccataa gggacaaaag gctcgttccg
    22741 gcggaaaggt aagggttggt tttgaaggcg gccagatgcc gatggcgcgt cgtcttccca
    22801 aacgagggtt ccgcaatcct ttcagaaaag aatatattgc cgtcaatatt ttggatttgt
    22861 gcagaaaatt ctccaaaggt gcggtgattg acgaggcggc tcttctgcaa agcggcctga
    22921 ttaaaacaga aggcgcccat atcaaaattc tggccaaggg agacattgat tttcctgtga
    22981 cattaaaaat tgcccgaatc agcccggccg caaaagataa aatcatcgct gcgggcggtt
    23041 cggtggaaga ggtaatttga tttgttagaa gggctgggca gtgtatccaa gattccggag
    23101 ttgcaaagaa ggattttatt cacgattctt cttttgggtg tttatcgaat cggcgtttat
    23161 attcccactc cgggaattga taatgtagcg ttactggctt attttgaaca tgccaaagga
    23221 tcgttgttcg gtctgatgga tatgtttgca ggcggagcgt tcagcaattt ttctattttt
    23281 gctctgggca ttatgcccta catcagttct tcgattattc tgcagttgct gacgattgcg
    23341 gttccgcatt tggagcgact gtccaaagaa ggtgaatcgg gccgccgcaa gataacgcaa
    23401 tatacccgtt acgggacggt tcttttaagt atgattcagg ggttcgggat tgcttttggt
    23461 ctgcagcaga tggccgggcc atcgggagct ccgattgttt tgaatccggg tatcggtttt
    23521 gtgctgatga caatgattac gctgacctcc ggtacggcgt ttattatgtg gctgggtgaa
    23581 gtcatcactg aaaagggcat tggcaacggt atatcgctga tcatttttgc cggtatcgtt
    23641 tgccggattc cggcggcaat cggcaatacc ttcaggctgg ccactgccgg tgaactgggc
    23701 gtactggtta ttctcttaat caccgttttg atgatcgcgg tggtaggggc gattgttttt
    23761 gtggaaggcg gtcagcgccg aattccggtt caatatgcaa aaagagtcgt tggccggaaa
    23821 atgtatggcg ggcagacaac ccatctgcct ctgaaagtga atacgtccgg agtaatcccg
    23881 ccgatttttg cttcgtcggt gatcatgttt ccggcaacga tagccaattt tgctccgcag
    23941 acctggatga aggatatcgc cgcctatttg gtgccgggaa gactggcgta cgaagtgctt
    24001 tatgtggcgt ttatcttctt tttctgcttt ttttatacgg cggttacgtt caaaccggac
    24061 gacgttgcgg agaatatgaa aaaatacggc ggttacgtac cgggcatcag accgggaaag
    24121 aaaaccgcgg aatatattga aaacgtactg gaaaaactga ctttcagcgg ggcgatttac
    24181 gtttccatca tctgcgtgct gcccagcgtt ctgattgccc agttaaacgt tcctttttat
    24241 tttgggggaa cggccttgtt gattgttgtc ggggtggcga tggatacggc cagccagatc
    24301 gagtcgcatc ttctgacacg gcaatacgag ggattcatga agaagggcca tattgcccga
    24361 aggagataac ggaagaatat aatggtcatt ctgaagcttc cggaagaaat tgaaagggca
    24421 agagccagta accgaattgt tgccgaggtt ttaagcagac tgagagaaaa ggtgaagccg
    24481 ggtgttaaga ccaaagacct ggataaattt gccgaggaga tcgcggaaaa aagaggagcc
    24541 cggccggcat ttaaaggcta tcatggttat cctcactctt tatgcgtatc gatcaatgaa
    24601 gtggtcgttc acggaatgcc ttccgagcgg gttttggagg aaggagatat tgtcgggctt
    24661 gattttggcg ttttttatca gggatttttc ggtgacgcaa ccgtaaccct gccggtggga
    24721 aaggttacgg caaaagcctt aaggctcatg caggttaccg agcagagctt atacgccggc
    24781 atcgcacagg ccagagaagg caacaggctg ggagatatat ccgctgctgt gcaatctgtg
    24841 gtggaggaag ccggatattc ggtggtccgc gactttgtcg gtcacggcat cggcaaggat
    24901 cttcatgagg agccgcagat tcccaatttt ggccagaagg ggcgcgggat cgaactgaag
    24961 aagggaatga tcctggccat tgagccgatg gtgaatgagg gaaaatacaa ggttcaggtg
    25021 ttgccggacg gctggacggt ggtcacaaaa gacggaagtt tgtcggctca ctttgagcac
    25081 tctattgcga tcacagagca tggtcccgac atattgagcc ggctgaatta ttagctttgg
    25141 aatacaaaag gaattatggc caaagaggaa gcaattgaag tcgaaggaag agtgattgaa
    25201 cccctgccca atgccatgtt ccgggtggaa ctggacaacg gacacaaggt tcttgcgcac
    25261 atttccggaa agatgcgcat gcattttatc aagatcctgc cgggcgacaa ggtgacggtg
    25321 gaactttcac cgtatgattt gacccggggc cggattactt acaggacgaa gtaaaaatga
    25381 ggagtttaaa tcgtgaaggt tagatcatcg gttaaaaaaa tatgcgaaaa atgcaaaatt
    25441 gtcaaacgca aaggtgtttt gcgggtgatt tgcgaaaatc ccaagcataa acaacgtcag
    25501 ggttagtttt tttatattca ggaggtttta ggtggcacga atttcaggtg ttgatttacc
    25561 gaaaaataaa agaatggaaa ttgctttaac gtatatttat ggcattggca agaccagagc
    25621 aaagcaaatc ctgaaagatg ccggggtgag cccggacaca cggacagatg atcttgccga
    25681 ttccgaaata tcggcgatca gaggcatcat cgataaagaa ggcaaagtgg aaggcgatct
    25741 gcgccgggat atatccatgt caatcaaacg tctgatggat gtcggaactt atcgtgggtt
    25801 gagacaccgc aaggggcttc ccgtccgggg ccagagaacg cataccaacg gacgtacccg
    25861 aaaaggtccg cggcgcgcca ttgcgggcaa gaaaaagtaa atttatgatt tccaagtgga
    25921 ggtagaatgg ctaaggcagt cagaaaaacc ggcaagaaaa aagaaaaaaa gaacatcacc
    25981 gacgggattg cccatattca gtcgacgttt aacaatacca ttgtcaccat aaccgatatg
    26041 agtggtaacg tgatcgcatg gtcgtcatcc ggattgcagg gattcaaagg atcgcgcaag
    26101 agcacgcctt ttgccgctca gatggctgcg gaagatgtgg tgcgcaaagc caaagaacag
    26161 ggcatgcgaa aagttcaggt ttatatcaaa ggtcccggcg ccggcagaga atcggcactg
    26221 cgctcgctgc agatcgccgg tctgacgata acgttgattc gcgatgtgac gcccgtgccg
    26281 cataacggat gccggccgcc gaaacgtcgc cgggtgtaac gattaacgaa caatattaat
    26341 ttcaaggaaa tatagggagg caatttttgg caagatatac ggattcctca tgcaggttgt
    26401 gtcgccgcga aggattaaaa cttttttcaa agggtgaccg gtgttactcg gaaaaatgtt
    26461 cctttgaacg aagagattat gttccgggag atcatggtca gatgcataaa aaacagcgtt
    26521 ctgattatgg ggtccagttg cgggaaaaac aaaaactcaa gagaatgtac gggcttttgg
    26581 aaaaacaatt cagaagttat ttcgaaaagg ccgataagca aaagggtatt acaggcacca
    26641 atttgctgat actgctggaa agaagaatgg acaacatggt tttccgcatg ggatttgcca
    26701 attccagaat tgaatcccgg cagttgatca gccacggcca ttttctggtc aacggcaaac
    26761 cggttaacat cccgtcttat ctgctcaagg ccggggatga agtttctgta aaggaaaaca
    26821 gtcgcaaggt gacccgcatc atgggagccc ttgaaacggt tgtcaggcga ggtgttccgc
    26881 aatggctgga attggataaa gacaatttca atgccaagat taaaacactg ccggtgcggg
    26941 aagatttaac catgccgata caggaacagc ttattgtcga gctttattcg aagtaaaaaa
    27001 actattttca taaaccagct actataagga tgggtttaat tatgcaaagg aactggtcaa
    27061 gtttgattcg tcccaagcgt attgatgttg atgaagcgac acatacgaat ttctatggag
    27121 agtttaccat tcagcctctg gaaagaggat ttggtatcac gttgggcaat gccctgagaa
    27181 gggtactgct ttcttccata cagggctctg ccattgtatc agcgaaaatt gaaggggttt
    27241 tgcacga
//
LOCUS       DAOSME010000029        25829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2416_length_25829_cov_18.565880, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000029 DAOSME010000000
VERSION     DAOSME010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 25829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25829
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2416_length_25829_cov_18.565880"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4627
                     /locus_tag="PLG98_06035"
     CDS             <1..4627
                     /locus_tag="PLG98_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32850.1"
                     /translation="FNVTAYPVFDEKNTAIIGFVHIINDVTELKQAWEIVNASEMRLK
                     AQYQGSPLAIFIWQKQDKDFVLIDFNKMAFALSQGLVKKFINKTARQLYKKRPDILKD
                     IQDCFAKKTTIKKELFSERFIPGTYIKASYSFVPPDLVMVHIEDISERKQAEAALRES
                     ESLYRMLTEKMSDIVWTMDMSMRTTYVSPSVKKVLGFTPKERLRQTVEEQLTPESLAA
                     AVNIMDRELSDEKQGKSNPEKNVTLTLEYYHKNGSTRWLETTVSGLRNEQGKLTGLHG
                     LSRDITQRKRMEDELIESEKKYHSIVDNSTDAILLTIPDGDVLSANAAACKMFDRTEE
                     DIRRAGRSGLMDLSDPRLPAALEERKQTGRFRGELTFVRKDGSKFEGEISSVIFTDRK
                     GTPLTSMIIRDISERKQAEEALKQRDLLFKKLSAHVPGMIYQFVKKPDGTYCLPFSTD
                     AIRTIFGCFPEDVAHDYSPILHAVHPEDVDDFLNSIEYSTQHMTDWKHEYRVQLPDGT
                     VKWMLGQSTPEKLPDGTIVWHGFNADITDQKNIQESLKATQDNYRRLFEDHSAVKLIM
                     DPETGAILDANFAAANYYGWTREEMKQMKISQINILSPDEIKHEMDKVMQEHKVHFEF
                     RHRRADGSIRDVEVYSSRVRRNGSYVLHSIIHDVTERKQAERELKQAQAYNEAILKSI
                     PGILYLIDENGRLVHWNKQFEILTGYDAHEIQDSFVLDWFKDRQNDLLNIKAGIDTIM
                     TRGSASNVEANLQTKDGRLIPMLYTGVRFDIGGKHHILGVGTDITERKVAEEKLLREE
                     KRFRSFAEQSSDIIALVDKNGTMLYENPAVEKLLGYKQEERIGQDTFLNVHPDDLEDL
                     MRCFQILFTDKNVPVQKTETRIRHKDGTWLTFEISGSNVVNNDVIEFITVSLHDITER
                     KRIQQELLESRNYAEKLLRTANALIVVLDTEGNVVSLNEAGEKITGYRAGDIAGLNWF
                     DLIVPKNRYPYVWEAFKKWKFSNDLPESMENPIVTKSGEERLISWRNSTIIEKGGFAG
                     ILAFGMDVTERKKMEESLQSWMRRYELIVAASGQVAYEYTLSTGRIIWGSSIANVLGY
                     DMGEISGGFGQWEILLHPDDRDATIQSLEAAEKACGFWESQYRMKHRKGHYVWIRDRG
                     FFVPDAEGKAYCQLGMLEDITSLKQAEEELKRTLLMNKAILESVPGILYLYNDTGHLV
                     QWNRQFEVLTGYSGDELQGKYVMDWFGGLEPDTSNIKKGISDVMTKGYSSAEARMITK
                     NGDHLFMFFTGVKLTVSGQDYLLGIGLDISERKKAEMEIVRLNESLEEKVQERTRELE
                     AFSYSVSHDLRAPLRTIEGFSLALVEDYSDKLDNQAKDYISRIRRATEIMSELIDDML
                     KLSRITRTEIDKADINLSNIALTVVDELRKAHPDRPVKVTMPDSLTDYADSRLIKIVL
                     ENLLGNAWKFTGKKTDAEIEFGSTQQNGKKVYFVRDNGAGFDMEYVDKIFAPFQRLHN
                     IEEFPGTGIGLATVRRIISRHGGTVWAESVPHQGATFYFTLGYPPPA"
     gene            4705..6462
                     /locus_tag="PLG98_06040"
     CDS             4705..6462
                     /locus_tag="PLG98_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE32851.1"
                     /translation="MNEVLRVLIVDDSDDDTNLVLRKLRSGGYQPEWERVDTHDAMKN
                     ALEKKTWDVILCDYKMPGFSAPAALKLAQEINPNIPFIVVSGAIGEETAVEVMKAGAH
                     DYVMKDKLAKLSVAVKREIAEAKIRRDKKSMEDMLKRSENNFRNSLDESPLGVRIVTS
                     DGELLYANREILNMFGYDSIDELKSISHLQRYTPETFVEYEKRIEMRKHGEYVPPNYE
                     VSIVRKDGTIRHLEVFRKQVLWNGQMQFQALYNDITERKQAEKNLRESEKRYRVVVEN
                     ANEAILITQNMKILFANTVAINNTGYPMEELTSMNFSDLIHPDDIGMVRGYHDRRIKG
                     DAIPSVYSFRIVCRDKSVKWVELNTTVIEWEKKRAALNFLKDITVSKQLEEERSQSFI
                     KIKKTLDATVSAIASMVETRDPYTTGHQKRVSILARAIAEEMGLCMDVVEFIETAAII
                     HDIGKLSVPSEILSKPTKLTNLEFELIKTHSQSGYNILKDIEFPWPVADAILQHHERM
                     NGTGYPQKLQGYDILLESRILAVADVVEAISSHRPYRPGLGIDFALEEITKNKGVLYD
                     ENVVDACIKLFREKHFVLS"
     gene            6640..7902
                     /gene="hisS"
                     /locus_tag="PLG98_06045"
     CDS             6640..7902
                     /gene="hisS"
                     /locus_tag="PLG98_06045"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOE32852.1"
                     /translation="MEKITAIRGFKDILPDDALAFCRIESLARTIFDSFGFREIRVPI
                     LEKTELFKRSIGETTDIVEKEMYTFTDRDNDQITLRPEATASVIRAYIEHNMSANEPV
                     TKLFTFGPMFRRERPQKGRFRQFNQIDVEVFGDDKPQTDAEVIFMLMHFLNSAGLRNL
                     QLEINSLGCSACRPEFSKAVIDFLKDRQSRLCPDCQRRIHTNPLRVFDCKVPSCAETL
                     AGAPQIIDFLCSDCTQHFAQVQSFLADLQTPFVINARMVRGLDYYTRTAFEVTTGILG
                     AQNAVAGGGRYNGLVSFLGGPDVTGIGFAVGFERLVACLPENRKDTYTTDLFIAALGA
                     NAQHIAFGLTNRLRVCGLSAEMDYSDKSLKSQMKRADKLNSAYALIFGDKEIEEKQVV
                     LRNMQTKQQQDIPMNGLHESIIKIIKER"
     gene            7909..9681
                     /gene="aspS"
                     /locus_tag="PLG98_06050"
     CDS             7909..9681
                     /gene="aspS"
                     /locus_tag="PLG98_06050"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOE32853.1"
                     /translation="MAEFLTTDKRTHYCGNLDASLIGQDVVLMGWTHRRRDHGGVIFV
                     DLRDREGMVQVVFNPDNAKAHKEAHRIRSEFVLAVKGKVRRRPDGMENPSLKSGEVEV
                     LVSDLDIMNESKTPPFSFDDEEISENIRLKYRYLDLRRPAIQKNLFLRSRMAAAARRY
                     FEENGFIEVETPFLGKSTPEGARDYLVPSRIYKGMFYALPQSPQIFKQLLMISGFDRY
                     YQIVKCFRDEDLRADRQPEFTQIDVEMSFITEDDIMQMMEGLMKAIFKSCLDIDLALP
                     LPRLTYADAVSRYGKDNPDVRFGMEIVDLTSIVKDSGFKLFAEVASSGGGVVKAIKAQ
                     QASVLSRKDLDGLKDVVAVYGANGLAWAKINAADWTSPVFKYLKPDEVQAIGNALDAR
                     EGDIIFFIADKPKVASDALGNLRLHLGKKLGLIDPKALAFTWITEFPLMEYSETEKRF
                     VSTHHPFTSPFLEDLPLLTADPGKVRARAYDLVLNGSEIGGGSIRIHHKDVQSQVFSA
                     LKLSDEDARLKFGFLLDALEYGAPPHGGLAFGLDRLTMIMTGAESIRDVIAFPKTQKA
                     ACLMTDAPSKVSIEQLMELSIKIV"
     gene            9782..11674
                     /locus_tag="PLG98_06055"
     CDS             9782..11674
                     /locus_tag="PLG98_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="HOE32854.1"
                     /translation="MAKIKKENKPTAAQKSKIKKAALVDVDQPNLYKEIFPYSEVSKI
                     VFDHQTVPMEPAQEIFITDTTFRDGQQSRPPYTAEQIASIFQLLSRLGGPNGVIRQSE
                     FFLYSKKDKDAVEKCMALNLRYPEITSWIRASKDDVPLVKQFGLKETGILTSVSDYHI
                     FLKLNKKRSQAMNDYLGIVKTILDLGIIPRCHFEDVTRADIYGFCIPFAQQLMKLREE
                     SGIDIKIRLCDTMGYGITYPGAALPRSVDKLVHAFIHDAGVPGHLLEWHGHNDFHKAM
                     INATTAWLFGCSAANSTLLGLGERTGNPPIEGLIIEYIALKGRNGIDTTVITDIADYF
                     EKEIGVKIPHNYPFVGSGFNVTSAGVHADGLIKCEEIYNIFDTRKILKRPITTTITDK
                     SGNAGIAFWINQRFNLEGNKTVDKRHPGVSKIHKWVMEQYESGRITSISPEEMEKKVR
                     LYLPERFMSELEKIKYIAEKAAVAAVTHIIEQPLMKTMDTAKQEPLMQKFVDEYPSVQ
                     FAYVVDMNGRKTTRNITDIADRAKYENYGVGTDQSDRQWFINPLRTGEVYVSDFFISK
                     MTGVLCFTVSAPIFNTDDEMVGIFGVDIKFEDWVKRAEDMEDMDHIALHEEYKEMKSK
                     AKHGHH"
     gene            complement(11794..13242)
                     /locus_tag="PLG98_06060"
     CDS             complement(11794..13242)
                     /locus_tag="PLG98_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOE32855.1"
                     /translation="MKTGLIVYMIALLVFYLGVSMFLPLFVALVYQDGSAFAFFVSIL
                     IACCLGGVVYLFTRGQKDFHLTHRDGLAIVTFGWIAAGFIGTLPYLLSGSIPDFTMAY
                     FESVSGFTTTGASILKDVQHLPPAVLFWRAQTQWMGGMGIIVLSIAILPFLGIGGMEL
                     YKAETPSPVVDKLTPRISSTAKLLWKIYIFLTLFLIILLLLGGMPLYDSICHSFAAMP
                     TGGFSTKNVSVGYYQSSYIDYVILLFMFIAGVNFSLHYKLVSGHPKQFFRDPEFRVYL
                     YITVIAILVVSANIYGTVYPRIQDALRYASFQVVSIMTTTGFVTADFEQWPVFSQLIL
                     FVFMFLGGMAGSTAGGIKIMRIILLVKHCYLEIFRIIHPHATTMVKLGRTPVSQTIMR
                     SIWGFFILYLVIFMIGAIALAALGLDIISALTAMATCLGNVGPGLGSVGPMDNYSVIP
                     VTGQWILIVSMLLGRLEIYTIIVLLMPAFWRK"
     gene            complement(13242..14600)
                     /gene="trkA"
                     /locus_tag="PLG98_06065"
     CDS             complement(13242..14600)
                     /gene="trkA"
                     /locus_tag="PLG98_06065"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOE32856.1"
                     /translation="MRVIIVGAGEVGYHIAKKLSDEKRDVVLIDKDPGKIRRINENLD
                     VQAFLGSGTSPKMLRQAGIQDAQLLVAATDMDEVNLIACLLARNLNRYAVKIARVRNQ
                     EYLQEKSLFSKDLLGVDLIINTQSMMVETILNLMDIPGASEVIDFLGGAVKLVSFLIF
                     EDSPLAGKKLSELSNQEGKLLVGAIVRGHKILIPNGNDTIQPMDLVYFVIKKSELDQT
                     LSRLHMPAKPMRNVIIVGAGGTGMAVAKALDQRRLNVKLIDQDGAKCKEANRLLEKVV
                     VIKGDGGDRDLLIEENARAMDMLIAVTDDEESNVLISLLAKELGTARTITRVSKLSYM
                     HVVSNVGLDTVINPRMAAVRAILQYIRRGKIISVAPLREEQAEAIEAEALETSDIVNV
                     PLSKVDFPPGAIIGAILRGDAIVIPTGSTVVLPHDHLIIFAVQKVINKVEKLLTVKLE
                     YF"
     gene            complement(14785..16755)
                     /locus_tag="PLG98_06070"
     CDS             complement(14785..16755)
                     /locus_tag="PLG98_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE32857.1"
                     /translation="MIDYAKELNPEQYRVVMEEGGPLLVLAGAGSGKTRTLTYRVARL
                     LESGVAQENILLATFTNKASHAMLNRVESLIHAETRNIMGGTFHHVAHLILRRHAHYL
                     GYKTNFSIVDSEDARQIISTCMAELKIDPKLSKFPKSNIVGDIISLSVNTETTVENVL
                     MSRYPFFLHLMDEIIQVASLYEQKKRELAVMDFDDLLSNCRMLLQENADVLKNLSGRF
                     QYVLVDEYQDTNLIQADLIDLLASTHKNLMVVGDDSQSIYSFRGADFNNIINFPHRYP
                     DCKIFKLETNYRSTPEILHLANLSITNNEKQFPKELKAVRKNGMRPVIVSAQNVVKQA
                     DFVAQRVTELMRGGVPLREIAVLYRAHYHSMEMQMEFVRRDIPFDMRSGIRFFEQAHI
                     KDVTSYMRVLVNPRDELAWRRLLIMYPKVGKATFNKIWHYLKKEDNPLNAVLSEDFIK
                     AMSKSAKPGLEECRKTIRQLLDLPENERIPERIIDVLLNRGGYRVYLQDNYSDASSRE
                     EDLIQLGNFSAQFLQMEDFLNELALLTNMAKEENIGEEDDEDKVALSTIHQAKGLEWS
                     YVFLIWCADGMIPLQKALKEQDGEEEERRLFYVAVTRAKDQLYLCYPALDYTRSSGVL
                     NMAPSRFIREIAPLSEYDADRPFEQWMLCDDY"
     gene            17239..17691
                     /locus_tag="PLG98_06075"
     CDS             17239..17691
                     /locus_tag="PLG98_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOE32858.1"
                     /translation="MKNSKGVPPQPSSRQHKDNIQELKSEDALEALQGLNPVTFTYNE
                     EALSKGVPPPDACSKHFKDNINYLNKEDAVKAVTALNPVTFTYKECPQEQRVGFIAED
                     VPHLVATNDRKGLYAMDIVAVLTKVVQAQQKTITDMRDELNELKAKVM"
     gene            18163..20247
                     /gene="fusA"
                     /locus_tag="PLG98_06080"
     CDS             18163..20247
                     /gene="fusA"
                     /locus_tag="PLG98_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE32859.1"
                     /translation="MAKYETKSIRNLAVVGHGGTGKTSLCESILYVSGKSERLGRVDD
                     ATSCMDYEPEEQKRRGSISSAANFAEWQKHKINFIDTPGDSNFAYDIKCSMSVVENAV
                     VVVDAVGGVEFQTQKVWELADEFKLPRIIFINRLDRERANFNAALDSIKNKLKRKATP
                     VCLPIGSEDQFNGIVDLIGMKAYLFSDNKGIGKAVDVPADMKDDVKLLHDSMVEDIAE
                     SDDELMNKYLESGELSDEELKAGLKKGVASGSFIPVICGSAVKGIGISMLLDLCVDAF
                     ASPVDRGAIKGTKPGTDNVEERQPNEDTPFSAIVFKTIADPYAGRLTLFRVYSGKLHS
                     DSPVYNSTRKMTEKFGHIFFLEGKNQKQTEELIPGDIAGVAKLKETTTGDTLCNEKSP
                     IIFEKVPVPPPIMSFAIEPKSRGDEDKIASSIHRLTEEDPTIQFTRNVETKEMILSGM
                     GQVHIEVTIEKMKRKFGVEVNLNTPKIPYKETIKGKTNVQGKYKKQSGGRGQFGDCWI
                     DIEPLPRGGGFEFHDKIVGGVIPGQYRPAVEKGIVEAAAKGVIAGYPVVDFKINLTFG
                     SYHTVDSSEMAFKIAGSMAFQKGVMDCQPILLEPIVNIEIEVPDEYMGDVIGDLNSRR
                     GRVQGMDAKGSNQIIKGQVPLAEILKYAPDLRSMTSGRGNFTYTHSHYEEVPAFIAEK
                     IIAAYKKDKEEE"
     gene            20264..20755
                     /gene="mog"
                     /locus_tag="PLG98_06085"
     CDS             20264..20755
                     /gene="mog"
                     /locus_tag="PLG98_06085"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HOE32860.1"
                     /translation="MISAGIITVSDKGSQGQRDDLSGPAIAEMLAGAAIEIRHTIIIP
                     DEKDQIRKTIIDCSDVKQLDLILTTGGTGVSPRDVTPDATLDVIDKQIPGMAEAMRHE
                     SMRVTPHAMISRAVAGIRGKSLILNLPGSPKSVRENLAVVLPALKHAIEKIKGDDSEC
                     AVP"
     gene            complement(20721..21236)
                     /locus_tag="PLG98_06090"
     CDS             complement(20721..21236)
                     /locus_tag="PLG98_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOE32861.1"
                     /translation="MMSKHPSHNKGPAETEVRFITDASLAGLAKWLRVLGYDTIVFHQ
                     TAGRSMMRCADKENRILLTRRADMVERQFSGSLFLIKDLEVGKQLKAVIERFALKIDR
                     DKMFGICLICNEKLLPVSREKVRDLVPPYVFENCSRYNECPRCKKIYWMGTHSRNAFR
                     FMEQHIPNHLP"
     gene            complement(21211..21519)
                     /locus_tag="PLG98_06095"
     CDS             complement(21211..21519)
                     /locus_tag="PLG98_06095"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOE32862.1"
                     /translation="MRVVYLILVILFSFLIVTFSLQNSARVQLQYYHYLNIDVPTYMI
                     IFASLLIGVIITAFWGIVERFRLNRTINRLNKLVRDLRREIQATEKPLMINDVKTPQP
                     "
     gene            complement(21521..22006)
                     /locus_tag="PLG98_06100"
     CDS             complement(21521..22006)
                     /locus_tag="PLG98_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOE32863.1"
                     /translation="METIFAPWRMEYIRGEKVNGCVFCKCSVRCDEYILFEGKTAYVM
                     MNKYPYVNGHLMIIPQRHVGQIEDLTQEEKKEIFDLLDASVKVLKEAMNPNGFNIGIN
                     IGKAAGAGVEEHFHVHVIPRWEGDTNFMSVVTNVRVIPEALDETVASLTPLFKKYCRE
                     A"
     assembly_gap    22140..22239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22588..22869
                     /locus_tag="PLG98_06105"
     CDS             22588..22869
                     /locus_tag="PLG98_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32864.1"
                     /translation="MNNLEKTLNKIAENILHFDEASLTSLWAQYKNKVENFSPSAEWE
                     KAVIIFSIINAVRAKNAIFNDNLLHKKSSAKAGTPQKKIKTKSYLTLVK"
     gene            22869..24878
                     /gene="ligA"
                     /locus_tag="PLG98_06110"
     CDS             22869..24878
                     /gene="ligA"
                     /locus_tag="PLG98_06110"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOE32865.1"
                     /translation="MDKSSDLKRIIDLRKTIEYHNRRYYQQDSPEISDAEYDRLMREL
                     QDLENRYPEAFSPSSPSQRVGAAPLSKFAPFHHPSPMFSLANAFSEGEIIEFDQRLKR
                     LAGVNDIGYIAEPKLDGLAVNLIYENGVFKQGATRGDGTTGEDVSQNLKTISSLPLFM
                     KKTSEYQLPSFIEIRGEVYIEKDPFEKLNQRRTQDGEEPFANPRNAAAGSLRQLDSRI
                     TARRPLNIFLYGIGEAKGIDFSTHWEALQILDGWGFPVNRVIEKASNIEACIHYFERI
                     AAQREKLPYEIDGVVLKVDDLSLQKILGNVSRNPRWALACKFPAAQAMTVVKDIIVGV
                     GRMGTLTPVAIMEPVNVGGVMVSRATMHNEDEVLKKDVRVGDTVVIQRAGDVIPEVVR
                     IVPEKRSSDTKQFKMPAHCPECGSEVVRYEGEVAHRCVNISCPAQLKEHIRHFASRGA
                     MDIEGLGEKVSAQLFDAKLIADPADLYFLTKEQLVDLDRQAEKSAQNLIEAIAGSKNP
                     SLDKFIYALGIRHVGERTAKLLANHYGSIKNLISTTQDELTAIHEIGPEIAESIVDFF
                     HEQKNINVLNKFFKAGINPQKKEVQTSAPLQGKSFVFTGTMENMGRNEAQTIVENLGG
                     TIHSSVTKKTTYVVAGSDPGSKLDKARSSGIQIISENEFLKLIGR"
     gene            24881..25153
                     /locus_tag="PLG98_06115"
     CDS             24881..25153
                     /locus_tag="PLG98_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOE32866.1"
                     /translation="MKQIHVHVRGRVQGVFFRAATQETARGLNLTGWVRNRSDGSVEA
                     VFEGEDDHVDIMLAWCRQGPPNARVEEVLIQEEPCSGLFLDFDIKY"
     gene            complement(25257..>25829)
                     /locus_tag="PLG98_06120"
     CDS             complement(25257..>25829)
                     /locus_tag="PLG98_06120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE32867.1"
                     /translation="RSFSVQAAISIENARLFELATTDGMTKLYVHRYFQLLLDQEIKR
                     SRRQDRKFSLVMMDIDNFKSFNDTYGHQLGDRVLKDVAAAARKASRSEDVAARYGGEE
                     FVMILPDTDSKQAVILAERIRAGVAAIEIPHDHQKLHVTISLGVSTYPDHSEIKEELI
                     RAADAALYESKHRGKNCVSVFEKKNEINLQ"
BASE COUNT         7235 a         6262 c         6088 g         6144 t
ORIGIN      
        1 ctttaacgtc accgcttatc ctgttttcga tgaaaaaaat accgccatca tcggctttgt
       61 ccatattatt aacgacgtta ccgaactcaa acaggcctgg gaaattgtca atgccagtga
      121 aatgcggctt aaagcgcaat atcagggaag ccccctggca atattcatct ggcagaaaca
      181 ggataaagac ttcgtcctga tcgatttcaa taaaatggcc ttcgccctct cccagggtct
      241 ggttaaaaaa tttatcaaca agacagcgcg ccagttatac aaaaaacgac cggatatttt
      301 aaaggacata caggactgct ttgcgaaaaa aacaaccatc aaaaaagaat tattctcgga
      361 acgctttatt cccggaacct atatcaaggc ctcttacagt tttgtgcccc ccgatctggt
      421 aatggtccat attgaagaca tctccgagcg aaaacaggca gaagctgcat tgcgcgaaag
      481 cgaaagtctg tacagaatgc tcacggaaaa aatgagcgac attgtctgga cgatggatat
      541 gagcatgaga acaacgtatg tcagcccgtc cgtcaagaaa gtgctcggct tcaccccgaa
      601 agaaagattg cgccagacgg ttgaagagca actgacgcct gaatccttag cggcagccgt
      661 aaacatcatg gatcgtgaac tttctgatga aaaacagggg aaaagtaatc cggaaaaaaa
      721 cgtcacgctg actcttgaat attatcacaa aaacggctcg acccgctggc tggaaacaac
      781 cgtcagcggg cttcgaaatg agcaggggaa actgaccggt cttcatggtt tgtcgagaga
      841 catcacgcaa cgcaaacgca tggaggatga gttaatagaa agcgaaaaga aataccattc
      901 cattgtcgac aacagcacgg atgccatttt gctgaccatc cccgacggag atgtcctttc
      961 ggccaatgcc gctgcctgca aaatgttcga ccgcaccgaa gaagatatcc gccgagccgg
     1021 cagaagcggc ctgatggatc tgagtgatcc gcgtttgccg gccgctcttg aagaacgcaa
     1081 gcaaaccggc cggtttaggg gtgaattgac ctttgtgcgc aaagacggat cgaaattcga
     1141 gggagaaatt tcctccgtga ttttcacgga tcgcaaaggc acccccttga cgagtatgat
     1201 catccgcgat atttccgagc gcaagcaggc ggaagaggcc ctgaaacagc gcgatctttt
     1261 attcaaaaaa ttatccgccc atgtgcccgg aatgatctac cagtttgtaa aaaaaccgga
     1321 cgggacgtat tgcctgccgt tttccaccga tgcaattaga actattttcg gctgttttcc
     1381 ggaagatgtc gctcatgatt attctccgat cttacatgcc gtacatccgg aagacgttga
     1441 cgatttttta aactccatcg aatattccac tcaacatatg accgactgga agcacgaata
     1501 ccgggtgcag ttgcccgacg gcactgttaa atggatgctg ggccaatcaa cacctgaaaa
     1561 gctgcccgac ggcaccattg tctggcacgg gttcaatgcg gatatcaccg atcaaaaaaa
     1621 cattcaggaa agtctcaagg caacgcagga taattatcgc agactctttg aagaccattc
     1681 ggcggtcaaa ctcattatgg atcccgaaac aggagcgatt ctggatgcaa atttcgctgc
     1741 agccaactat tacggctgga cgcgggaaga aatgaagcag atgaaaatct cgcaaatcaa
     1801 tattctttcc cctgacgaaa tcaagcacga gatggataag gttatgcagg aacataaggt
     1861 ccattttgaa ttccgtcatc gtcgcgccga cgggtccatc agagacgtcg aggtatattc
     1921 cagcagggtc agaaggaatg gctcttacgt tttgcattct attattcacg acgtaaccga
     1981 acgcaaacag gcggaaagag aattaaaaca ggctcaggcc tataacgaag ccattctcaa
     2041 aagcattccg gggattcttt atctgatcga tgaaaacgga cgtctggtac actggaacaa
     2101 acaattcgaa atcctgaccg gctatgacgc ccatgaaatc caagacagtt ttgttctgga
     2161 ctggtttaag gaccggcaaa acgatttatt aaacatcaag gcgggcatcg ataccatcat
     2221 gacccgtgga tcggcttcca acgtggaggc caaccttcaa acgaaagacg gacgattgat
     2281 tcccatgctt tacacagggg tcagattcga tatcggcggt aaacatcata tcctgggtgt
     2341 gggtaccgac atcaccgaaa gaaaagttgc agaagaaaaa ctgctccggg aagaaaagcg
     2401 tttccgttca tttgccgagc aatcctcgga cattattgcg ctggtggata aaaacggcac
     2461 aatgctttat gaaaatcccg ccgtggaaaa actcctgggg tacaaacagg aggagcgaat
     2521 cggccaggat acgtttctta acgtacaccc tgatgatctg gaggatttaa tgagatgctt
     2581 ccagattctg tttacggata aaaatgtgcc cgttcaaaaa acggaaactc gcatccgcca
     2641 taaagacgga acctggctca cctttgaaat atcgggaagt aatgtcgtca acaatgatgt
     2701 tatcgaattt attaccgtca gccttcatga catcaccgag cgaaaaagaa ttcaacagga
     2761 gttactggag tcgagaaatt acgctgaaaa actgctcaga acggccaatg cattgattgt
     2821 ggttctcgat accgaaggca acgtcgtttc attgaacgaa gccggagaaa agattaccgg
     2881 ttaccgggcc ggagacattg ccggtttaaa ctggtttgat ctgatcgttc cgaaaaaccg
     2941 ttatccctat gtctgggagg cattcaaaaa atggaaattc agcaacgatt tgccggaatc
     3001 catggaaaat cccattgtga caaaatcggg cgaagaacgc ctgatttcat ggagaaacag
     3061 cacgattatt gaaaaaggcg ggtttgccgg catcttagcg ttcgggatgg atgtaacgga
     3121 acgcaagaag atggaggaat ccctgcaatc atggatgcgg cggtatgaac tgattgtggc
     3181 ggcctccgga caagtggctt atgaatatac tttatccacc ggccggatta tctgggggtc
     3241 cagtatcgca aacgtcctcg gatacgatat gggagaaatc agcggcggct tcggacaatg
     3301 ggaaatcctc ctgcatcctg acgacaggga tgcaacgatt caatctctgg aggcagccga
     3361 aaaagcctgc ggcttctggg agtcgcaata ccgcatgaaa cacagaaaag ggcattatgt
     3421 atggatcagg gatcgcggtt tctttgttcc tgatgccgag ggcaaagctt attgccaact
     3481 cggcatgctg gaagatatca cctccctcaa acaggccgaa gaagaattaa aacggaccct
     3541 gttaatgaac aaagccattc tcgaaagcgt tcccggcatt ctttatctct ataacgacac
     3601 cggacatctg gtgcagtgga acaggcagtt tgaagtgtta acaggctatt ccggagatga
     3661 actacaggga aaatatgtta tggattggtt cggcggcctg gaacccgata catcaaacat
     3721 taaaaaaggc atatccgacg ttatgaccaa gggatattct tccgccgagg cacgtatgat
     3781 cactaaaaac ggggatcatc tttttatgtt ttttaccggc gtcaaactta ccgtctcggg
     3841 ccaggattat cttctcggca ttggcttaga tatcagcgaa cgcaaaaaag cagagatgga
     3901 aattgtcagg ctgaacgaat ccctggaaga aaaggttcag gagcgcacca gagagctgga
     3961 ggcgttttct tattccgtat cgcatgacct gcgggcgccg ttaagaacaa tcgagggatt
     4021 cagccttgcg ctggttgagg actacagcga taagctggat aatcaggcca aagattatat
     4081 cagccgaatc aggagagcga ccgaaatcat gagtgagctg atcgacgaca tgctgaaact
     4141 ctccagaata acgcgcacgg aaattgataa agccgatatc aacctctcca atattgcgtt
     4201 gacggtcgtt gatgaacttc gcaaagccca tcccgaccgt cccgtaaagg tgaccatgcc
     4261 ggattccctc accgattacg ccgattcgag attaataaaa attgtacttg aaaatctcct
     4321 gggcaacgcc tggaaattta cgggcaagaa gacggacgct gaaattgaat tcggctcaac
     4381 gcagcagaac gggaaaaaag tatatttcgt tcgggataac ggcgccggtt tcgacatgga
     4441 gtatgtggat aaaattttcg ctccgttcca acgcttgcac aacatcgaag aatttcccgg
     4501 aacgggaatc gggctggcca cggtcaggcg aatcatcagc cgtcacggag gaaccgtctg
     4561 ggcggaaagc gttccccatc aaggcgccac tttttatttt acgctgggct atcccccgcc
     4621 tgcataaaac acatttggaa gaaggccgtt ggatgacgga agacaaaaga tgattgcatt
     4681 gcataaatta agggagaaac gaatatgaat gaagtgcttc gcgtcctgat tgtggatgat
     4741 tccgatgacg ataccaatct ggttttacgg aaactgcgca gcggaggtta tcagcctgaa
     4801 tgggaacgag tggatacgca tgatgctatg aaaaacgctc tggagaaaaa aacatgggac
     4861 gtgatcctgt gcgattacaa gatgcccggc ttcagtgcgc cggccgccct gaaactggct
     4921 caggaaatca atccgaatat tccctttatt gtcgtttccg gcgccatcgg cgaggaaacg
     4981 gccgtcgagg tcatgaaggc cggcgctcac gactatgtca tgaaagacaa gctcgccaag
     5041 ctttctgtcg ccgtgaaacg ggaaatcgcc gaagcaaaaa tcaggcggga taaaaaaagc
     5101 atggaagaca tgctcaaaag aagcgaaaat aatttccgca attcgctcga tgaatctccg
     5161 ctgggcgtac gtattgtcac gtccgacggt gagttattgt atgccaaccg ggaaatttta
     5221 aacatgttcg gctacgatag catcgatgag ttgaaatcca tttcccatct gcagagatat
     5281 acgccggaaa cctttgtcga atacgaaaaa cggattgaaa tgagaaaaca cggcgaatat
     5341 gttccgccga attatgaagt cagcattgtc cgcaaggacg ggaccattcg ccacctggag
     5401 gttttccgca agcaggtttt gtggaacggc caaatgcaat ttcaagccct ttacaacgat
     5461 atcaccgaac gcaagcaggc cgaaaaaaac ctgcgggaaa gcgagaagcg ctatcgcgtc
     5521 gttgtggaaa acgccaatga agccattttg atcacgcaga acatgaaaat cctttttgcc
     5581 aataccgtgg ccataaacaa tacaggctat cccatggaag aactgacctc catgaatttc
     5641 agcgatctta tccatccgga cgatatcggc atggtcaggg gttatcacga cagaagaatc
     5701 aaaggcgatg ccataccgtc tgtttactct ttcaggatcg tttgccgcga caaatccgtg
     5761 aaatgggtgg agctaaacac caccgtaatc gaatgggaga aaaagcgtgc cgcgctgaat
     5821 tttttaaagg acataacggt cagcaaacaa ctcgaagagg aacgctccca aagttttata
     5881 aaaatcaaga aaacgctcga tgcaaccgta agcgctattg cctccatggt ggaaacccgg
     5941 gatccgtata caacgggtca tcaaaaacgg gtgtcgattc tggcccgggc cattgctgag
     6001 gaaatgggat tatgcatgga cgttgtggaa ttcatcgaga cggccgcaat tattcacgac
     6061 atcgggaaat tatccgtgcc ttcggagatc ctcagtaaac cgaccaagct caccaatctt
     6121 gagtttgaac tgataaaaac acattcccaa tccggttaca acattctcaa ggatattgaa
     6181 ttcccctggc ctgttgccga tgccattttg cagcaccatg aaagaatgaa tggaaccggt
     6241 tatccccaaa agctgcaagg ctacgatatt cttctggaat cgcgaatact ggccgttgcc
     6301 gacgtcgtcg aagccatttc gtcccacagg ccttaccggc ccggcctggg cattgatttt
     6361 gctctggaag aaatcaccaa aaacaaaggc gtgctttatg acgaaaacgt tgtggatgcc
     6421 tgcataaagc tcttccgcga aaagcacttt gttttgagtt aagaagcgcc tcccgtcaca
     6481 caaatgtatg cgtccaggat gcaatcgttt gtgcggtgcg aaagcgttga tttctttctc
     6541 attgcatcca ttgccccttc ccctgcttca acctattttc tcgttgccaa gaagacatga
     6601 atgtgttagt aatgcaccct taaaaatttg gttgggatta tggaaaaaat tacagcaatt
     6661 cgaggtttta aagatatcct gccggacgat gcgcttgctt tctgcagaat cgaatccctg
     6721 gcccgaacaa tttttgattc tttcggtttc cgggaaatac gcgtccccat tctggaaaaa
     6781 acagaacttt tcaagcgaag catcggcgag accaccgata ttgtcgaaaa ggaaatgtac
     6841 accttcaccg accgggacaa tgaccagatc accctgcgac cggaagccac cgcgtcggtc
     6901 atccgggctt acatcgaaca caacatgtcc gcaaacgaac ccgttacaaa gttatttaca
     6961 ttcggtccga tgttccgccg cgaaaggccg caaaaaggtc gattccgcca gtttaatcaa
     7021 atcgatgtgg aagttttcgg cgatgacaaa ccccaaaccg acgcggaagt gattttcatg
     7081 ttgatgcatt tcctgaacag cgcgggtttg cgcaatctgc aactggaaat caattccctg
     7141 ggctgcagcg cctgccgtcc cgaattttcc aaggcggtga tcgatttcct gaaggacagg
     7201 caaagccgtc tgtgcccgga ttgtcagcgg cgcattcaca ccaatcccct gcgggtcttc
     7261 gactgcaagg ttccctcctg cgccgaaacc cttgccggtg cgcctcagat cattgatttc
     7321 ttgtgttcgg actgcacgca gcatttcgca caggttcaat cgtttcttgc cgatttgcag
     7381 acaccgtttg tcatcaacgc cagaatggtc cgcgggctgg attactacac ccggacggcc
     7441 tttgaagtca ccaccggcat cctgggcgcc cagaacgccg ttgccggcgg agggcgttac
     7501 aacggcctgg tcagctttct gggggggccg gacgtaaccg gaataggctt tgccgtcggt
     7561 tttgagcgtc tggtcgcctg cctgccggaa aaccggaagg atacctatac aaccgatctt
     7621 ttcattgcgg cgctgggcgc gaatgcacag catatcgcct tcggtttgac caaccggctc
     7681 agagtttgcg gcttatccgc ggagatggat tactcggaca agagtctcaa gtcccagatg
     7741 aaacgagcgg ataagctcaa cagcgcctat gctctcattt tcggcgacaa ggaaattgaa
     7801 gaaaaacagg ttgtcttgcg caacatgcag acaaaacaac agcaggatat tcccatgaat
     7861 ggcctgcatg aatccataat aaaaatcatc aaggagaggt aaaaagtttt ggctgaattt
     7921 ttaacgactg ataaacggac acactactgc ggaaatcttg atgcttcact gatcggccag
     7981 gacgtcgttt tgatgggctg gacgcatcgc cggcgcgatc acggcggcgt tattttcgtc
     8041 gatttacggg atcgcgaagg catggtgcag gtggttttta atcccgataa cgcaaaagcc
     8101 cataaggaag cccatcgaat ccgttctgaa tttgttctgg ccgtcaaagg taaggtgcgc
     8161 aggcgaccgg acggcatgga aaatccgtcc ctgaaaagcg gggaagtcga agtcctcgtc
     8221 tccgatttgg acatcatgaa cgagtccaaa acgccgccct tctccttcga tgatgaagaa
     8281 atttctgaaa atatccgttt gaaataccgt tatctggatt tgcgccgtcc ggcgatacaa
     8341 aaaaatcttt ttctgcgcag cagaatggcg gcggcggcgc ggcgctattt cgaagaaaac
     8401 ggttttattg aagtggagac tccgtttctg ggaaaaagca ctccggaagg agcgcgcgat
     8461 tatctggtgc cgagccgaat ttacaagggc atgttttacg cgctgccgca gtcgccgcaa
     8521 atcttcaagc agttgctgat gatttccggt ttcgaccgtt attaccagat cgtcaaatgt
     8581 ttccgggatg aggatttgcg cgccgaccgg cagccggaat tcacgcagat tgacgtggaa
     8641 atgtctttca ttaccgaaga cgacatcatg cagatgatgg aagggctgat gaaagctatt
     8701 ttcaagagct gcctggatat cgacctcgcc cttcccctgc cccgtctgac gtatgccgat
     8761 gccgtcagcc gctacggaaa agacaatccc gacgtgcggt tcggcatgga aattgtggat
     8821 ttgacgtcca tcgtcaaaga ttccggcttt aagctttttg cggaagtcgc ttcttccggc
     8881 ggcggcgtcg tcaaagccat caaagcgcaa caggcctccg tactttcccg gaaagacctt
     8941 gacggcttga aagatgttgt ggccgtttac ggcgccaacg gtttggcctg ggccaagatc
     9001 aacgccgccg actggacatc gcctgttttt aaatatctta agcccgacga agtgcaggcc
     9061 atcggtaatg ccctggacgc cagggagggc gatattatct tcttcatcgc agacaaaccc
     9121 aaagttgctt ccgatgccct ggggaatttg cggcttcatc tgggcaaaaa actgggactg
     9181 attgatccca aagcgctggc cttcacctgg attacggaat ttcccctgat ggaatattcg
     9241 gaaacggaaa aacgttttgt ctccacacat catccgttca cgtcgccctt tctggaggat
     9301 ctgccgcttt taaccgccga tccggggaag gttcgcgcca gggcttacga ccttgtgctc
     9361 aacggttcgg aaatcggcgg gggcagcatt cgtatccacc acaaagatgt tcaatctcag
     9421 gtcttcagcg cactgaaact cagcgacgag gacgcccggc tgaaattcgg tttcctgctg
     9481 gatgccctcg aatacggagc accgcctcac ggcggtctgg cgttcggtct ggacaggctg
     9541 accatgatca tgaccggggc ggaatcgatc cgcgatgtca ttgccttccc gaaaacccag
     9601 aaagccgcct gtttgatgac cgatgcgccg tcaaaagtca gcattgaaca attaatggag
     9661 ttatcgataa aaatcgttta agcaaaatgc ccatacgcag aatcagcgcg tctgtaccgt
     9721 tcgtatgagc cgcgctgact tacaaaacca ggaatgaaca cattcaatac cggagatata
     9781 catggctaaa ataaagaagg agaacaaacc gacggctgcg caaaaaagta aaatcaaaaa
     9841 agcggcattg gtcgatgtgg accaacccaa tttgtacaaa gagatattcc cttattcgga
     9901 agtcagtaaa attgttttcg accatcaaac cgttcccatg gaaccggcgc aggaaatttt
     9961 cattacggat acgaccttcc gcgacgggca gcagtcccgc cctccctata ccgccgaaca
    10021 aattgcctcc atattccagt tattgagcag gctgggcggg cccaacgggg tcatccgtca
    10081 atcggaattt tttctctaca gcaagaagga caaagatgcc gtggaaaaat gcatggccct
    10141 caatctgcgt tatccggaaa tcaccagctg gatcagggca tccaaggacg atgttcccct
    10201 ggtcaagcag ttcggcctga aggaaacggg cattctgact tccgtctcgg attaccacat
    10261 ctttctgaaa ctcaacaaaa aacgaagcca ggccatgaat gattatctgg gaattgtcaa
    10321 aaccattctg gaccttggca ttattccccg ttgccacttt gaagatgtca cccgcgccga
    10381 catttacggt ttttgcattc cttttgccca gcagttgatg aagcttcggg aggaaagcgg
    10441 catcgacatc aaaatcaggc tctgcgacac gatgggttac ggtatcacct atcccggcgc
    10501 cgcccttccc cgcagcgtcg acaagcttgt ccatgcattt attcatgacg ccggcgttcc
    10561 cggccatctg ctggaatggc acggacacaa cgactttcac aaggccatga tcaatgcaac
    10621 caccgcctgg cttttcggtt gcagcgccgc caattccacg ctgctgggtc ttggcgaaag
    10681 aaccggtaat cccccgattg aagggttaat tatagaatat atcgccctga aaggaagaaa
    10741 cggtatcgac accaccgtca tcaccgatat tgcggactat ttcgaaaagg aaatcggcgt
    10801 gaagataccc cataattatc cgtttgtcgg gtcgggcttc aatgtcacca gcgccggtgt
    10861 ccatgccgac ggtctcatca aatgcgaaga aatttacaat atctttgata cgcgaaaaat
    10921 tttaaaacga ccgatcacca cgaccatcac ggacaaatcc ggcaacgccg gtattgcgtt
    10981 ctggatcaac cagcgtttta atctggaagg caataaaacg gtggacaaga ggcatcccgg
    11041 tgtttccaaa attcacaagt gggttatgga acagtatgaa tccggacgca ttacttccat
    11101 atcgcctgaa gaaatggaaa agaaagtccg tctttacctg ccggaacgct tcatgtccga
    11161 gctggaaaag atcaaataca ttgcagaaaa agccgctgtt gccgccgtta cgcacatcat
    11221 tgaacaaccc ttgatgaaaa ccatggatac ggcaaaacag gaacctctga tgcagaaatt
    11281 cgtcgacgaa tatccttccg ttcaatttgc ctacgttgtg gatatgaacg gccggaaaac
    11341 gacacgaaat attaccgata tcgccgatcg cgccaaatat gaaaattacg gcgtgggaac
    11401 cgaccagtcc gacaggcagt ggtttatcaa tcccctgcgc accggcgagg tctacgtctc
    11461 cgatttcttc atttccaaaa tgaccggtgt tttatgcttc acggtttccg cccctatttt
    11521 taatactgac gatgagatgg tgggtatttt cggtgtggat attaaattcg aagactgggt
    11581 caagagagcg gaagatatgg aggacatgga tcacatcgcg ctgcatgaag aatacaaaga
    11641 aatgaaatcc aaagccaagc atggccacca ttgatcataa ccgcgtcttg taaagacgtg
    11701 aaacattgct ccggcgcggc tccgtcagat agagatgttt atctttcaaa caaaacgcaa
    11761 cggcctcgcg aaccgaacgc tttcagccgg gcattatttg cgccagaagg caggcatcag
    11821 cagaacaata atggtgtaaa tttccagacg tcccagcagc atcgacacaa tcagaatcca
    11881 ctggcctgtc acgggaataa ccgaataatt gtccatgggg ccgacactgc ccaaccccgg
    11941 cccgacgtta cctaaacagg ttgccatagc ggtcagcgct gaaatgatat ccaggccgag
    12001 agccgccagt gcaatagctc cgatcatgaa aatgaccaga tagagaataa agaagcccca
    12061 aatgcttctc atgattgtct gggaaaccgg cgtcctgccg agtttgacca tcgtcgtggc
    12121 atgaggatga ataattctga atatttccag ataacaatgc ttgacaagca ggatgatcct
    12181 catgatcttg ataccgcccg cggttgaccc ggccatccct cccagaaaca taaagacaaa
    12241 gagtatcaat tgcgaaaaga caggccattg ttcaaagtct gcggttacaa atccggtggt
    12301 tgtcatgatg gaaacaacct ggaatgatgc atagcgtaaa gcatcctgta tccggggata
    12361 aaccgtcccg taaatattgg cagacacgac gagaatggca atcacggtga tgtaaaggta
    12421 aacgcgaaat tccggatccc ggaaaaattg tttcggatgc ccgctgacca gtttataatg
    12481 cagcgagaag ttcacgccgg caatgaacat gaacaggaga atgacataat cgatataact
    12541 gctctggtaa tatccgacac tgacgttttt cgtcgaaaat ccacccgtcg gcatcgcagc
    12601 aaaactgtgg cagatggaat cataaagcgg cataccgccc agcaacagta aaataatcag
    12661 aaaaagcgtg agaaaaatgt aaattttcca gagcaatttg gctgtgctgg aaatccgcgg
    12721 cgttaatttg tccacaacgg gactcggcgt ctcagcctta tagagttcca tgccgccgat
    12781 ccccaggaac ggaagaatgg cgatggataa cacgataatc cccatgccgc ccatccactg
    12841 cgtttgagcg cgccagaaaa gtactgcggg cggcaaatgt tgcacgtcct tgagaatgct
    12901 cgccccggtc gttgtaaacc cggagacgga ttcgaagtaa gccattgtaa agtccggaat
    12961 cgatcccgaa aggagatacg gcagcgtccc gataaacccc gcggcgatcc atccaaaggt
    13021 gacaatggcc aagccgtccc gatgcgtcaa atgaaaatct ttttgacccc ttgtaaaaag
    13081 atagacaacg cctcccaggc aacatgcaat caggatggaa acaaaaaaag cgaaggcgct
    13141 gccgtcttga tacacaagcg ctacaaacag cggcagaaac atggaaacgc cgagatagaa
    13201 taccagcagt gcaatcatgt agacgattaa accggttttc atcaaaagta ctccagcttg
    13261 acggtaagaa gtttctccac tttgttgatg actttttgca cggcaaagat aatcaggtgg
    13321 tcatgaggaa gcaccacggt actgccggtg gggataacaa ttgcatcccc gcgtaaaata
    13381 gcgccgatga ttgcgccggg aggaaagtca accttcgata agggaacatt gacaatatcg
    13441 gatgtttcta aagcttccgc ttcaatggcc tcagcctgct cttcccttaa aggagccacg
    13501 gaaatgattt tgccccgccg gatgtactgc agaatggcgc ggacggcagc catgcgggga
    13561 ttgatgaccg tatccaatcc gacgttggag acgacatgca tgtaactgag tttgctgacc
    13621 ctggtgatgg tgcgtgctgt tcccaattcc tttgccaaaa gcgagatcag gacattgctt
    13681 tcttcatcgt cggttacggc aatcagcatg tccattgcgc gggcattctc ttcgataagc
    13741 aagtcccggt caccgccgtc tcccttgatc acaacgactt tttcaagaag cctgttggct
    13801 tccttgcact tggccccgtc ctgatcaatc aatttgacat tcaaacgtct ttgatcgagc
    13861 gctttggcca cggccatgcc cgttcctccc gctccgacaa tgatcacatt tctcatcggc
    13921 ttggcaggca tgtgaagacg ggagagtgtc tggtctaatt ccgatttttt gatgacaaaa
    13981 taaaccaggt ccatgggttg aatggtatca ttgccattgg gaataaggat tttatgtccc
    14041 ctgacgattg cgccaaccag caattttcct tcctgattgc tcagctcgct gagtttttta
    14101 cccgccagcg gggaatcctc gaaaatcaga aagctgacca acttgacggc gccccccaga
    14161 aaatcaatca cctccgatgc gcccggaata tccataaggt tgagaatggt ctcgaccatc
    14221 atggactggg tattgatgat cagatccacg cccagaagat ctttgctgaa taatgatttt
    14281 tcctgaaggt attcctgatt tctgacacgc gctattttta ccgcatatct gttcaggttg
    14341 cgcgccagca ggcacgcaat gagattgact tcgtccatat cggttgcggc cacaagaagt
    14401 tgcgcatcct gaatgcctgc ctgccgcaac attttgggac ttgtgcccga tccaagaaaa
    14461 gcctggacat caaggttttc gttgatccgt ctgatcttgc ccggatcctt gtcaattaag
    14521 acaacgtctc ttttttcatc ggaaagtttt ttggcaatgt ggtatccgac ctctccggcg
    14581 cccactataa taacgcgcat atttcccccc tccacgttac aacggtatca acttatataa
    14641 tggacacccc gggaattaca acataaaaag cttgataacg ccgccccaga tcattcactt
    14701 tacggacggc tttttcggat tccagagtga ccaggatgat aggattttta aaaacatatt
    14761 gctctttttt gaatgtgcaa tttcttaata atcatcacat aacatccact gttcaaaagg
    14821 ccggtccgca tcgtattctg aaagaggcgc gatttcccgg ataaaccggg agggagccat
    14881 attgagaact cctgaggaac gcgtataatc cagggcagga tagcataaat agagttgatc
    14941 tttggcccgg gtcaccgcca catagaagag ccttctttcc tcttcctcgc cgtcctgctc
    15001 tttcaacgcc ttctgcaacg gaatcatgcc atccgcgcac cagatcagaa aaacatacga
    15061 ccactccagc cccttggcct gatgaatggt gcttaaggca accttgtctt catcgtcctc
    15121 ttccccgatg ttctcttctt tggccatatt ggtcagaagc gccagttcgt tgaggaagtc
    15181 ctccatctgc agaaactggg cggaaaaatt tccgagctga attaaatctt cctctctgga
    15241 tgatgcatcg gaatagttat cctgaagata aacccggtag ccgcctctgt ttaacaacac
    15301 atcgataatc ctttccggaa tccgttcatt ttcaggaaga tcgagaagct gccggatcgt
    15361 tttccggcat tcttccagac cgggttttgc ggatttggac atcgccttga tgaagtcctc
    15421 actcaagaca gcattcagcg gattgtcttc cttcttcaga taatgccata ttttattgaa
    15481 ggtggccttg cccaccttgg gatacatgat cagcaaccta cgccaggcca gttcgtcgcg
    15541 gggattgacc agaaccctca tgtaggacgt tacgtcttta atatgcgcct gttcaaaaaa
    15601 acgaatgccc gaacgcatat cgaaaggaat gtcgcgccgg acaaattcca tctgcatttc
    15661 catcgaatga taatgcgccc ggtaaagcac ggcgatttcc cggagcggca cgccgccccg
    15721 catcagttcc gtaacccgct gggccacaaa atccgcctgt ttaacaacat tctgcgcgga
    15781 aacaatcacc ggcctcatgc cgtttttgcg cacagccttg agttccttgg gaaattgttt
    15841 ttcgttattg gtgatgctta aatttgccag atgcagtatt tccggcgtac tgcgatagtt
    15901 tgtttccagc ttgaatattt tgcagtccgg ataacgatgg gggaaattaa tgatgttgtt
    15961 gaaatcggca ccacgaaagg agtaaatact ctgggaatcg tctccgacca ccatcaggtt
    16021 tttgtgcgta gaggccagca gatcgatcag atccgcctga ataagattgg tatcctgata
    16081 ttcatccacc aggacatact gaaaccggcc ggaaagattt ttcagaacat cagcgttttc
    16141 ctgaaggagc atgcgacaat tgcttaataa atcgtcaaaa tccatgacgg ccagctccct
    16201 cttcttttgt tcataaagag aagcaacttg aataatttca tccattagat ggagaaaaaa
    16261 cgggtatcgt gacatcagaa cattttcgac ggtagtttcc gtgttgacag acaggctgat
    16321 gatgtcccct acgatattac tcttggggaa cttgcttaat ttcgggtcta tttttaattc
    16381 ggccatacag gtggaaatga tctgtcgggc atcctcactg tccacaatcg aaaaatttgt
    16441 tttatagcca aggtaatgcg catgccttct caaaatcaga tgggcaacat ggtgaaatgt
    16501 gccgcccatg atatttctcg tctcggcgtg aatcaggctc tccacccggt tgagcatggc
    16561 atgagaggcc ttattggtga aagtggccag taaaatgttt tcctgcgcaa cgccgctttc
    16621 cagcaggcgg gcgactcgat aagtaagcgt tcgggttttt ccgcttccgg ccccggccag
    16681 aaccagcagc ggaccgccct cttccatgac cacacgatac tgttccggat tcagttcttt
    16741 tgcataatca atcatcaacg cgtctttata gtaaaaggtt tagatattca agcgaataac
    16801 ggcaaaaggt ccacataata tgaacttcct catatttctc tcgccctgtt cccgcatttc
    16861 tctgcacacc gcatccgaca tacaatagat tcggcgctaa acccgatgca aaagaaggat
    16921 tctgagttaa aaaaagaatt gacaaaaaat atgaaattgt ttattaaaat agcatattaa
    16981 aaatccaaaa gacatcacag gttttcaaaa aatagctttt aatgattagg cgctgctgtc
    17041 actctctacc cgtattgtgg aaagcgtgat cacaaaaaca aagcagaata taatcaggaa
    17101 cagaaatatg cttctaaagg cccaagaatt cgaaactgtt ggcatgagcc aagagccaaa
    17161 caaatactcg gccccctttt tttgataaat attaaagttt aacatattaa aaataataag
    17221 acatgaaagg agataacttt gaaaaattca aaaggagttc ctccacaacc gtccagcaga
    17281 cagcataaag ataatattca ggagttgaaa tccgaagatg ccctagaagc gcttcaaggg
    17341 ctcaatcccg ttacgttcac gtataatgag gaagcacttt caaaaggcgt tcctcctcca
    17401 gatgcttgca gcaaacactt caaggataat attaactacc taaacaagga agatgctgtg
    17461 aaagcagtca cggcgcttaa tcctgttacg tttacataca aggaatgtcc tcaggaacaa
    17521 cgtgtaggct tcattgccga agatgttccg catcttgttg ccacaaacga ccgtaaagga
    17581 ctatacgcta tggatatcgt ggcagtacta acaaaagtag tgcaagcgca gcagaaaaca
    17641 attaccgaca tgagagatga gttgaatgaa cttaaagcta aagtaatgta aaatacggtt
    17701 catcttcaag ggaaatatga gaagtatttc aaaggcagga tcattaatga ttctgccttt
    17761 ttaaatttca atcttttatc aacaacgtct ccggattgaa cggcaaatca ttttttgcgg
    17821 aataagctat tttatccata atagattgga tatatatttc attgccttat gtcggaaaaa
    17881 tatggggtta aatttttaat ctcgaccttt aaagccgaca aatcgtgaaa gcaagaaagg
    17941 agacaagtct gtatttagct catgcaacct aacattgagc tcacgaccac atgaattcca
    18001 tcaccccttg attattttca tcatattcaa gcacttccct gaacggttca tcaagtctct
    18061 ggaatagcaa atatcaaatt tgtcttgatt ttacttccct tatacgttaa acagcccaaa
    18121 ttcaatttga attgtctttt taaagcattg aaaggagttt tcatggcaaa atacgaaaca
    18181 aaatcaatca gaaatttagc tgtggtcggt cacggcggaa ccggaaagac atctctttgt
    18241 gaatccatat tgtatgtttc agggaaaagt gaacgattag ggcgcgttga tgatgcaacc
    18301 tcctgcatgg actatgagcc tgaagaacaa aaacggcggg gctccatcag ttccgcggca
    18361 aattttgccg aatggcaaaa gcacaaaatc aattttattg atacgccggg tgactccaat
    18421 ttcgcctatg atataaaatg ctccatgagc gttgtggaaa atgctgtcgt cgtggttgac
    18481 gctgtcggcg gtgttgagtt ccagacacaa aaagtttggg aacttgccga tgagttcaaa
    18541 ctaccccgta ttattttcat taacagactg gatcgggaac gcgctaattt caacgccgcg
    18601 ctcgacagca tcaagaataa attaaagaga aaagcaaccc ccgtctgtct tcccatcggt
    18661 tccgaagatc aatttaacgg cattgttgat ttaatcggca tgaaagctta cctgttcagc
    18721 gacaataagg gcatcggcaa agccgtcgat gttcccgcgg acatgaaaga cgacgtaaag
    18781 ctcctgcacg attccatggt ggaagacatt gccgaatccg atgatgaact gatgaataaa
    18841 tatctggaaa gcggtgaact ttccgatgaa gagttaaaag ccggattgaa aaaaggcgtt
    18901 gcgtcgggaa gtttcattcc ggtcatttgc ggctccgcgg ttaaaggcat cggcatctcc
    18961 atgcttctgg acctgtgcgt ggatgctttc gcatcgcctg tcgatcgcgg tgcaatcaag
    19021 ggaacgaaac cgggaacgga caacgttgaa gaacgccagc ccaatgaaga tacgcccttc
    19081 tcggctatcg ttttcaagac gattgccgat ccttacgccg ggaggctgac tctcttccgt
    19141 gtttattccg gaaagcttca ttccgattcg ccggtttata attcaacccg gaaaatgacg
    19201 gaaaagttcg ggcatatctt tttcctcgaa gggaaaaacc agaaacaaac ggaagaattg
    19261 attcccggcg atatcgccgg cgtggccaaa ttaaaggaaa caacaacagg cgataccctc
    19321 tgcaatgaaa aatctcccat cattttcgag aaagtccctg ttcctccgcc gatcatgtcg
    19381 tttgcgatcg aaccgaaatc gcgcggcgat gaagacaaga ttgcttcctc cattcaccgg
    19441 ctgacggaag aagacccgac cattcagttc acccggaatg ttgaaaccaa ggaaatgatt
    19501 ctctccggca tgggacaggt gcatattgaa gtcaccatcg aaaaaatgaa aagaaaattc
    19561 ggcgttgaag tcaatctgaa cacgcctaag atcccctata aagaaacgat taagggaaaa
    19621 accaacgtac agggaaaata caagaagcaa tccggcggac gcggacagtt cggcgattgc
    19681 tggatcgata tcgaacccct gccccgcgga ggcggttttg agtttcatga caaaatagtc
    19741 ggtggcgtta ttccgggaca atatcgtccg gccgttgaaa aaggcattgt ggaagcggcg
    19801 gccaaaggcg tgatcgccgg ttatccggta gtggatttta aaataaatct tacattcggt
    19861 tcttatcaca cagtagactc gtcggaaatg gcctttaaaa tcgccggttc catggctttt
    19921 caaaaggggg tcatggattg tcaaccgatt cttctggaac ccatcgtcaa tatcgaaatc
    19981 gaagttcccg acgaatacat gggggatgtg atcggagacc tcaacagccg acgcggccgc
    20041 gttcagggca tggatgccaa aggttccaac cagattatca aaggccaggt tccgctcgct
    20101 gaaattttaa agtacgcacc ggatctcaga tccatgacca gcggcagagg caatttcacg
    20161 tacacgcatt ctcattacga ggaagttccc gctttcatcg ctgaaaaaat cattgccgcg
    20221 tataagaaag ataaggaaga agaataatca caggaatcag ctcatgatca gtgccggaat
    20281 tataacagtc agtgacaaag gctctcaagg ccaacgtgac gatttaagcg gccctgccat
    20341 agcagaaatg ctggcaggcg ccgccattga aatcaggcac accatcatca ttccggatga
    20401 aaaagaccag atcagaaaaa caatcatcga ttgttcagat gtcaaacagt tggatttgat
    20461 tctcaccacc ggaggcaccg gtgtcagccc ccgcgatgtg acgcccgatg cgacactgga
    20521 cgtcattgac aaacaaatcc ccggcatggc ggaagccatg cgccatgaga gcatgcgcgt
    20581 tacgccccat gccatgattt cccgcgccgt ggcgggaatt cgcggaaaaa gcctgatatt
    20641 aaatcttccc ggaagtccca aaagcgtccg tgagaatctt gcggttgtcc tgccggcgct
    20701 gaaacacgcc atcgaaaaaa tcaagggaga tgattcggaa tgtgctgttc catgaagcgg
    20761 aaggcgtttc gggaatgcgt tcccatccag tagatttttt tgcaacgggg acattcattg
    20821 taccgggaac agttttcaaa gacataaggc ggaaccagat cccgtacctt ttcccgcgaa
    20881 accggaagca gtttttcatt gcagatcaga caaatcccga acattttatc cctgtcgatt
    20941 ttcaaggcga atcgctcgat gaccgccttg agttgcttgc cgacctcaag atctttaatt
    21001 aaaaaaagac tgccggagaa ctgccgctca accatgtccg ctcttctggt caacagaatt
    21061 ctgttttcct tgtccgcaca gcgcatcatg gaacgtccgg cggtctggtg aaagacaatc
    21121 gtgtcatacc ccagaaccct tagccatttg gccagaccag ccagactggc atccgtgata
    21181 aaccggacct ccgtttcagc tggacctttg ttatggctgg ggtgttttga catcattgat
    21241 catcagcggt ttttcggtgg cctgaatctc cctgcgcaaa tcgcggacca gtttatttag
    21301 gcggttgatt gtgcggttga ggcgaaaacg ttccacgatg ccccaaaacg ccgtgataat
    21361 aacgccgatc agaagagatg cgaagatgat catataggta ggtacgtcga tgttgagata
    21421 gtgatagtac tgcaattgaa ctctggccga attctgcaga gaaaacgtaa caattagaaa
    21481 agaaaaaaga atgactagga tcagataaac gactctcatt ctatgcctcc ctgcaatatt
    21541 ttttaaaaag aggcgtaagg ctggcaacgg tttcatcgag tgcttccgga atgacccgga
    21601 cattggtaac gacgctcatg aaatttgtat cgccctccca tcgcggaatg acatgcacgt
    21661 gaaaatgttc ttcgacgcct gccccggcgg ctttgccgat attgatgccg atattgaatc
    21721 cgttggggtt cattgcctct ttgagcactt taacggaagc gtccagaaga tcaaagatct
    21781 cttttttctc ctcctgcgtc aaatcttcaa tttgcccgac atgccgctgg gggatgatca
    21841 tcagatgtcc gttcacataa gggtatttgt tcatcatcac gtaagcggtt ttgccttcaa
    21901 acaaaatata ctcgtcacat cggacggaac acttgcagaa gacgcaaccg ttgacttttt
    21961 cccctctgat atattccata cgccaaggcg caaaaattgt ttccatattt gaaactcgcc
    22021 gtttttttaa atttatattt ttattatcct acccgttatc tgtttgtcaa tttctaatat
    22081 gccgatggtt tttgaagaat tgaaaaagct gtccgccgcc gaaccgggat taaagaaaan
    22141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tccaccatat cgccggcatg
    22261 caaaatcatg tccgcatcgt caaaatagcc ggcaatacgt ttttgcatct tcggatcata
    22321 atcatcaaga tgcgtatcgg atatgactcc tatttttatt tttttgcctt ccataacgta
    22381 aaaaccatgg cgtcatttaa ctgcttgcgg caaaaaaaac aagctttaaa attagtgaaa
    22441 cacgccaaga atgatattca atattcagga atgaagcgaa ctgaaacact ttggttgaat
    22501 tatcctgtgt gctatccaaa tatgatttca ttaaaggaat aaagctttaa tttgacatta
    22561 ccggcaagta aagttaatat ttcgatcatg aacaatctgg aaaaaactct gaacaaaata
    22621 gctgaaaata tcctgcattt tgacgaggct tcgctgacct cgctctgggc tcaatataaa
    22681 aataaggtcg aaaacttttc gccttccgcc gaatgggaaa aagcggttat catcttctcg
    22741 attatcaatg ccgtgagagc taaaaacgca atttttaacg ataatttatt gcataaaaaa
    22801 tcttctgcaa aagcgggaac accgcaaaaa aagataaaaa caaaatctta tctgacactt
    22861 gttaaataat ggataaatca tcggatttaa aaagaattat cgatctgaga aaaacgatcg
    22921 aataccataa caggcgttat tatcaacagg attcgcctga aatttccgat gccgaatacg
    22981 atcggttgat gcgtgagctt caggacctgg aaaatcgtta ccctgaggca ttttcaccgt
    23041 cttctccctc tcaaagagtc ggcgcagccc ctttgagtaa atttgccccc ttccatcatc
    23101 catcgccgat gttcagtctg gccaacgctt tttccgaagg cgaaattatc gaatttgacc
    23161 agcgccttaa aagactggcc ggagttaacg atataggcta tatcgccgaa ccgaagctgg
    23221 atggcctcgc ggttaatttg atctacgaaa acggcgtttt caagcaggga gcgacacgcg
    23281 gtgacggtac gaccggcgaa gatgtttcgc aaaatctcaa aacgatctct tccctgccct
    23341 tgtttatgaa aaaaacatcg gaatatcagc ttccttcttt cattgaaatt cgcggcgaag
    23401 tttatattga aaaagacccc tttgaaaaac tcaatcagcg ccgtacccaa gacggtgagg
    23461 aaccctttgc caatccccgc aatgcggccg ccggatcgct gcgtcagctt gattcaagaa
    23521 ttacggcacg ccgccccctc aatatcttcc tgtatggcat cggcgaggca aagggcattg
    23581 atttttcaac gcactgggaa gcactgcaaa tccttgacgg ctgggggttc cctgtgaatc
    23641 gcgtgattga aaaagcatcg aatatcgaag cctgcattca ttattttgag cgcattgccg
    23701 ctcaacggga aaaactgccc tatgagatcg acggcgttgt tctcaaggtt gatgacctat
    23761 ctttacaaaa gatactcggc aatgtctcgc gcaatccgcg ctgggcgctc gcctgcaaat
    23821 ttccggcggc acaggctatg acggtggtca aagacatcat cgtcggtgtc ggacgcatgg
    23881 gaactctgac acccgtcgcc attatggagc cggtcaatgt cggcggtgtc atggtcagcc
    23941 gcgccactat gcataatgaa gatgaggtcc ttaaaaaaga cgtgcgcgtc ggagataccg
    24001 tggttattca acgcgccgga gatgtcattc ccgaagttgt ccggattgtt ccggagaaaa
    24061 gatcctccga tacgaaacaa tttaagatgc ccgcccactg ccccgaatgc ggctccgaag
    24121 tggtccggta tgaaggagaa gtggcccacc gttgcgtgaa catatcctgc ccggcccagt
    24181 taaaagaaca tatccgccat ttcgcgtctc gcggcgcgat ggatattgaa gggctcgggg
    24241 aaaaagtttc tgcgcagctt tttgacgcca agctgattgc cgatcctgcg gatctttatt
    24301 ttctgactaa agaacagctc gtcgatcttg accggcaggc ggaaaaatca gctcaaaatt
    24361 taatagaagc catcgccgga tcgaaaaatc cttctctgga taaattcatc tatgcattgg
    24421 gtatccgcca cgtcggagaa cgcaccgcca agcttctggc aaatcattac ggcagcataa
    24481 agaatcttat ttcgacaacc caggacgaat taacggccat ccatgaaatc ggcccggaaa
    24541 tagccgaaag catcgttgat ttttttcatg aacaaaaaaa tatcaatgtc ctgaataaat
    24601 ttttcaaggc cggcataaat ccgcagaaaa aagaagttca gacgagcgca cctttgcagg
    24661 ggaaatcgtt tgtttttacc ggtaccatgg agaacatggg cagaaacgaa gcccaaacga
    24721 ttgttgaaaa tctgggaggc acaattcatt ccagtgtaac gaagaaaacg acctatgtcg
    24781 tcgccgggag cgatcccggt tcaaaactgg acaaggcaag atcttccgga attcaaatca
    24841 tcagcgaaaa tgaattttta aaactgatag gcaggtaaca ttgaaacaaa ttcatgttca
    24901 tgtcagaggc agagttcagg gagttttttt ccgggcggcc acccaggaaa cggccagggg
    24961 gttgaatctc accggctggg tgcgcaaccg gtcagacgga agcgtggaag ccgtctttga
    25021 aggtgaagac gatcacgtgg acataatgct ggcgtggtgc cgccaggggc cgccgaatgc
    25081 gcgcgtggaa gaagttctta ttcaggaaga accctgctcc ggcctgtttt tggatttcga
    25141 cattaaatac tgaacgcatc cttggacaaa cttgctcccg gcgtatcaat cacaattcga
    25201 tccggtcgtg cagaaccgcg gattgatgac acaagatttt ccttattgcg caggagctat
    25261 tgaagattga tttcgttttt cttctcgaac acactgacac agttttttcc cctgtgcttt
    25321 gactcataaa gggcggcatc cgccgcgcga atcagttctt ccttgatttc agaatgatcg
    25381 ggatacgtcg acacgcccag actgatcgtg acatgcaatt tctgatggtc atgagggatt
    25441 tctattgccg caacgccggc ccggattctc tcggccagaa tcacggcctg ttttgaatcg
    25501 gtatccggca gaatcatgac aaactcttcc ccgccgtaac gggccgccac atcttcggaa
    25561 cgcgatgctt tccttgccgc cgcagcaaca tctttcaaaa ccctgtcacc cagctgatgc
    25621 ccgtaggtgt cgttgaaaga tttgaaattg tcaatatcca tcatcacgag tgaaaacttc
    25681 ctgtcctgac ggcgcgaccg ttttatttcc tgatcgagca gaagctgaaa atagcgatgg
    25741 acatacaact tggtcatacc gtcggtcgtg gccagttcga aaagcctcgc gttttcgatg
    25801 gatatggcgg cctgaacgga aaaacttcg
//
LOCUS       DAOSME010000030        25094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2510_length_25094_cov_22.560006, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000030 DAOSME010000000
VERSION     DAOSME010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 25094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25094
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2510_length_25094_cov_22.560006"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLG98_06125"
     CDS             <1..920
                     /locus_tag="PLG98_06125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE32868.1"
                     /translation="SFPPNTYRLNPYNGWHPDMRELEQKVRSHKAIVGILVINPDNPT
                     GFVYPEETLRNIVRIAKENDLFVVFDETYINIVYNGKNTVPLSDIVGSVPAISMKGIS
                     KELPWPGSRCGWMEVYNAGKDPIFDRFINTILHQKMSEVCSTTLPQLAIPKIMTHPEY
                     DKYLQERRRHYEKLSDIAYNILKDVPCMMVNKTDGAFYMTIVFNESGLNSNQKLHIKN
                     GEIREFVENLVKEPIEHDKRFVYYLLAATGICVVPLTSFFTPLLGFRMTLLDKDVDEF
                     EHTVKTIAEKFTEYIDSSSKGMQTAIKLY"
     gene            991..1959
                     /locus_tag="PLG98_06130"
     CDS             991..1959
                     /locus_tag="PLG98_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32869.1"
                     /translation="MIAVQGYPMTLSVLLFIAGIVLLWAGTEFVLQRVPILAGWLRVS
                     PLVVTVLLLAVMTSLPEFCVSLFASMRGQPAAAVGNVVGSNFVTLTFVAGICALWRPI
                     IVGPTLRERESSWMILSAALLLVIAMDGVISRIDGLILLMAYGPYLKGTIDEARQQRK
                     EAPVVSMAKPWLHGIIFVAGIAMIVLGSSWIVENGTMLARRLGMNDLLIGVTFYAFGT
                     SLPELAIALGAVLRRQADVTLGEIYASNIFTGLAVIGCLCLIKPLPVEPIIVQRDLPM
                     LIVAGVLLQMFVTTGRKFVRLEALVMIAIYVLFLAAQFMGFSISLS"
     gene            complement(2085..3674)
                     /locus_tag="PLG98_06135"
     CDS             complement(2085..3674)
                     /locus_tag="PLG98_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE32870.1"
                     /translation="MSLSDRNNPYTFDDFLKWRQGVDYYRDDPFIQKVVHHFTGDIRQ
                     QVDAEARACSQKASFRWRDFADSIARPEKRPFMLHYDGHHNRIDRIVRPHETEIMEKE
                     IFSEAFFADTTSPWVKLIKMYVIYQNGEACIGCPVTCTEGLVMLLEKYADSPETKEIL
                     KHCKDGINGDFAIGAQYLSEIQGGSDVPANVLEAVNENGTWKLYGTKFFCSATHADYA
                     VVTAKPVGSEKVALFVVPSWLPGNKEKEMRNGYTVDRIKWKMGTSELTTAELTFNGAV
                     AYPVGPLDKGVANVVGIVLTYSRLTVGLSAAAYMTRAVREAKKYTEFRNAFGLTIGQF
                     PLVAVQVNRIDRMAKRTTAGAFKLYRDFLTLEGGLKGGLATDEPEAVRKNRFNIRELI
                     MLQKMAAAWDCTDVIRESMSIFGGHGVMEDFSSLPRLYRDSAINELWEGPRNVLLTQI
                     HRDLQKVSGWYKPAEFVRHILSGLNESTIKELADEITELVAYPNLFQPDEKTMDICRR
                     WDAFCHRFFHAYQDLALKEVE"
     gene            3803..4414
                     /locus_tag="PLG98_06140"
     CDS             3803..4414
                     /locus_tag="PLG98_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE32871.1"
                     /translation="MKKKNTFAELKNNEKRARQGVIVEAAQRVFAVKPFNKVSIRDIA
                     REAGISHASIYRYFPDQQSLFVEAFLHGAEEITAILDRMIKNSRKNPIEKVADAYLSY
                     LIDNDQYFRMMTRFMLDGSLSADMVEKLNASERRIFDQFDVLFRKMNLPEPVRPLSHA
                     FFAALNGVLITFRNYPGRSAKAVHQHMHNIVRVIVSKFCVPSA"
     gene            complement(4571..4906)
                     /locus_tag="PLG98_06145"
     CDS             complement(4571..4906)
                     /locus_tag="PLG98_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOE32872.1"
                     /translation="MNKNLYVGNLSYKITGDDLKSNFSEAGEVVSATIIKDKFSGQSK
                     GFGFVEMKTEEGAAEAIKKFHGGTLDGKEITVNEARPKKEFSGGHRGGGNRGGGGGFN
                     RGGRRDGRY"
     gene            complement(5179..5958)
                     /locus_tag="PLG98_06150"
     CDS             complement(5179..5958)
                     /locus_tag="PLG98_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE32873.1"
                     /translation="MTFRISTRFIRVWQRNLTVYRESWKINFMPPLLEPLFYLLAFGI
                     GFSGLIGSVTYAGTEISYVMFIAPALLAINIMNNSFFENTFGSFVRMYYQKTFDAMMA
                     TPLTVDEIVTGEIVWGATKSVIGTIIMLIVIGFFGLISYPAGLLIVLVAFIGGFAFGS
                     VAMIFTGLVGKIELFNLPVFLFITPMFLFSGTFFPISGLPLWAQYFALTLPLTHLVNL
                     VRALGLGAFNAEALAGFCYLIIFCLITFPLALSVMRKKLIK"
     gene            complement(5955..6884)
                     /locus_tag="PLG98_06155"
     CDS             complement(5955..6884)
                     /locus_tag="PLG98_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE32874.1"
                     /translation="MIETRPIEPVIVARNLEKVFGAFKAVDDISFHVVPGVCFGILGP
                     NGAGKTSTIRMLYGFSPLTAGDIFVFGLNIKTHDRKIKARIGVCQQENTLDPDLTVMQ
                     NFEVFARYFDIGKKTALARATTLLKFFALEQRQNARATELSGGMVRRLVIARALINQP
                     DLIILDEPTTGLDPQSRHQVWERLEQLKNQGMTILLTTHYMDEAARLCDNLVIMDHGK
                     ILAQGSPRSLIREYTGGHIIEVAEPDEALRAFSQEKHDRVEDLGHRLIIYAANGHELY
                     QELCDRFSQKSCILRSATLEDVFLKLTGRELRE"
     gene            7332..8951
                     /locus_tag="PLG98_06160"
     CDS             7332..8951
                     /locus_tag="PLG98_06160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE32875.1"
                     /translation="MKVKFLGAAREVTGSCFIIETDQARFAVDCGMHQGGADVETRNW
                     DTEAYDPSHIDFFLITHAHIDHSGLLPRMVQNGFKGPVYATEPTGELIKILLLDSAHI
                     QETDAKSKTRRMQRNGRTEKSEPLYSTPDAEAVSPLIKKITYNQNFTPAAGIKINFQD
                     AGHILGAAILELFIEENGTSTKLVFSGDIGRRHQLLMEDPVTISDADYLFMESTYGDR
                     DHKGEEDSLNEMAEAIQYSYSRGEKIIIPAFAVERTQEMLYSLYLLDIKGKLPKDMPV
                     FLDSPLAIKATEIFRRHHTYLDADTQNILKNGEDPLDLPQLKFSSSTEQSMRINEMEG
                     SAIVISASGMCNAGRIRHHLRHNLWRPGASIVFVGFQAQGTPGRKIVEGAKKIRIFNE
                     DIAVKAKIWTIGGFSAHAGQRQLLDWLGHFQNKKMPVYLVHGEAGAQDTLASLIRERL
                     GFEVAIPEYLEEINIKAGTRPQEIKQPRPSRPPLELAPLLADLKAKIDYINARVDKIQ
                     SLPESEQQEVIQLLRQVNQNMDDIKPDLQTG"
     gene            complement(8992..9558)
                     /locus_tag="PLG98_06165"
     CDS             complement(8992..9558)
                     /locus_tag="PLG98_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOE32876.1"
                     /translation="MDKKAILWPVIISVVIHMTLLAVAGMIDLNDKIKPVNVLSVSIQ
                     EPAPAQPSPEKENPAQGNPSSSVTKQQNDPTPDDAWREDTINLGSLDTKYIHYLTRVK
                     NKILRIWKYPQTSFERNEEGNVVVRMSIDADGRLAAVTLVSSSGSADLDNGALGVVQE
                     AAPYEPLPGSYNLSRLHIVASFNYKIMD"
     gene            complement(9780..10106)
                     /locus_tag="PLG98_06170"
     CDS             complement(9780..10106)
                     /locus_tag="PLG98_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HOE32877.1"
                     /translation="MAEKKGLSKPVKLKGDLAELLGAKALPRTEITKKLWDYIKANKL
                     QTTKVNGKPENAGKNIVIDAKLIKIINNTKVKTSSGKVVDFTKLKEGQTIDMMQIASV
                     VSANVE"
     gene            10351..12006
                     /locus_tag="PLG98_06175"
     CDS             10351..12006
                     /locus_tag="PLG98_06175"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOE32878.1"
                     /translation="MANRSTQKRAVKIPRGKALLCDPGLNKGTMFSDTERDVLGLRGL
                     LPPRVFSPSDRTVHIINNVRRKTTDLEKYLYLSGLQNRNERLFYRVLIEHIQELMPVI
                     YTPTVGQACQEYSSIFSEPRGLFISKNDRGSIKKILKRWPQKKVRVIVVTDGERILGL
                     GDLGANGMGIPVGKLALYAACAGIDPEACLPVTLDVGTNNPELLAAPDYIGLPQPRVR
                     GREYDQLVDEFMAAAANVFPGVMIQMEDFGNANAFRLLEKYRSKYCMFNDDIQGTASV
                     ALAGLYAALRITGGSISRQRLLFLGAGEAAIGIGDLVSSAMMEEGLSLPEAHSNCWFV
                     DSKGLVVKGRTDLVEHKKPYAHDYPFHRDLLSAVEALKPTAVIGVSGQPGTFTPEIIK
                     AMGRINRRPIIFALSNPTSKAECTAEAAYRHTEGRAIFASGSPFGEVSMEGKTFVPGQ
                     ANNAYIFPGVGLGVLTSEAVRVTDEMFTAAAKSLNEQVSADDLKIGRIYPSLTTIREV
                     SAKIALAVAEVVFKRSLTRMQAPADLRGNIRATMYEPDFGTYI"
     gene            12043..12579
                     /locus_tag="PLG98_06180"
     CDS             12043..12579
                     /locus_tag="PLG98_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOE32879.1"
                     /translation="MKKKDKRFVGSLAFVFALLFVISSAGAALADAVSAKIGFVDIAK
                     IMRDSRAAQNARAVYQKDFESKKATLKAKGDRVSSLEKDLSNTKQDSPALKEKREKLA
                     QEFKEFKRLESDLNEQLKKKDAELTRKILMDVQEILKQFSKNEKYSMIFEKKAVLVVD
                     DGFDVTDKIIKIYDAQKK"
     gene            12945..14474
                     /locus_tag="PLG98_06185"
     CDS             12945..14474
                     /locus_tag="PLG98_06185"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOE32880.1"
                     /translation="MIQQPGFDNEKYIQEQTTEILKRVNRFNNKLYLEFGGKLLYDYH
                     AARVLPGYDPNVKMRLIRELKDKADIVLCIYAGDIERKKIRADFGITYDADALKLIDN
                     LRTWGINVLGVVITRFEEQPAAIIFKNKLERRGISVYTHRFTKGYPTDVETIVSDEGY
                     GANTYIETDKPLVIVTGPGPGSGKMATCLSQLYHDYRRGIKSGYAKFETFPIWNIPLK
                     HPVNVAYEAATADIRDFNLIDPFHLDAYSEKSVNYNRDVEVFPVLKRILEKITGEPSF
                     YKSPTDMGVNRAGFAIIDDAITQKAAKMEIIRRYFRYRCEYAMGFADRETVQRVELFM
                     KDFNLEPEERPVVQPAREAAVTAPVETRDYERGNEGIFCGAALELKNGKIITGSNSSL
                     MHAATSMVLHAIKHLAEIPDKIKLLPPQITDSVKNLKTEILDEKSVSLDLEEALIAIS
                     ISATNNPAAQLALEKLRELRDCEAHMTHIPTPGDEAGLRRLGINLTTDPNFSTNDLFI
                     T"
     gene            14629..15627
                     /gene="gap"
                     /locus_tag="PLG98_06190"
     CDS             14629..15627
                     /gene="gap"
                     /locus_tag="PLG98_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOE32881.1"
                     /translation="MSVKIAINGFGRIGRLVFRAGYQNKDVDFVAVNDLADAKTLAHL
                     LKYDSTHGILDADISVANNAIIVDGKELKTFSVREPETLPWKDLGVDVVLESTGKFTD
                     KTGAGKHMEAGAKKVVVSAPAKNPDVTFVLGVNQEVYDKNKHHIISMGSCTTNCLAPI
                     VKILHQEFGIEYGLMTTIHSFTNDQVVVDEPHRDLRRARAAALSMIPTTTGAARAIAE
                     VIPEMKGKLDGLAIRVPTPNVSLVDFVATLSKSTTRNEINDKFREYAQSAMKGILACS
                     EEELVSKDFNGNKHSSIVDMPNTTVIGGNMVKILSWYDNEWGFSNRMLELLSFIMK"
     gene            15642..16820
                     /locus_tag="PLG98_06195"
     CDS             15642..16820
                     /locus_tag="PLG98_06195"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOE32882.1"
                     /translation="MKYIDQIDMKGKRVFLRVDFNVPMDKEGNVTSDKRVRASIPTIT
                     YALEKGAKLIVASHLGRPKGKRVAEMSLKPVVKVLSDLLKKEVLFLEDCVGPVVEQGV
                     ARLQDGQIILLENLRFQPGEDKNDAQFAKQLAALCDVYIDDAFAVSHRAAASNSAITE
                     FVPVCAAGFLLKNEVEYFKKAMVNPAKPLAAIIGGAKVSDKIGLLENLIEKVDKIIIG
                     GGMAFTFLRAAGYETGKSLCEEDMLDTARKIVDKAKQKNVQFLLPVDAVIAQAAEAQA
                     EARICGVQDIPKEWIGLDIGPATIALFSEALKGVKTVIWNGPMGMFELAPFSKGTFQL
                     AQAVADSGALSILGGGDTDTAIKKAGLTSKMSYISTGGGAFLEWLEGKTLPGIAALEK
                     "
     gene            16860..17615
                     /gene="tpiA"
                     /locus_tag="PLG98_06200"
     CDS             16860..17615
                     /gene="tpiA"
                     /locus_tag="PLG98_06200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOE32883.1"
                     /translation="MRKWIVAGNWKMHNTIAESIMLAKAIQDGSSDIQGGAVILAPPF
                     TALSAVGEAIKGSCLELAAQNMHYEDKGAFTGEVSPLMLKDIGCRYVIIGHSERRKYF
                     HEQNADVNLKVNKALSDGLIPIMCVGETDEEREKGITRDVVGRQVNHGLKGVEKIDPI
                     VIAYEPVWAIGTGKVATPAQAQDVHSFIRGILKESYGDVAGKISILYGGSVTRDNIGD
                     LIAMEDIDGALVGGASLKAEGFLGIIKTVAGGR"
     gene            complement(17806..17991)
                     /gene="tatA"
                     /locus_tag="PLG98_06205"
     CDS             complement(17806..17991)
                     /gene="tatA"
                     /locus_tag="PLG98_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOE32884.1"
                     /translation="MFGMGWQELVIVLIIVVIIFGVGKLPEIGSALGRGIKNFKKSIN
                     EQNEITDNSSKKEEDKK"
     gene            complement(18009..18806)
                     /locus_tag="PLG98_06210"
     CDS             complement(18009..18806)
                     /locus_tag="PLG98_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOE32885.1"
                     /translation="MFIEQTPSDNSQKLKMKRESLGLSPDDVFKKIRIRTRYIEAIEN
                     SEFDQLPDPVYTKNFIKTYARFLNIDENPVLQDYDAYLNAHREEQTPPPEPPPETKSF
                     FASFAARKNYWGILLVLIIFISIWLILKQNQSVPEDINTPAAATRAPEATLPAAPALT
                     DSQTPATVPAPQTGVTDNPPATTDAKKSVLLITAGQETWLRIKPDNSPPVQLLLKPGE
                     KFESSEEFFNLDIGNAGGIKIQYKGRSIENLGKPGEVIHIRLPQVKQ"
     gene            18956..19741
                     /locus_tag="PLG98_06215"
     CDS             18956..19741
                     /locus_tag="PLG98_06215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOE32886.1"
                     /translation="MRELKSFAVEMARNAGALLKKKFNKTHKIQYKGDINIVTEADKM
                     SEHLIIKSIRRNFPEHGILSEESPAIAGAGKKRWIVDPLDGTTNYAHGYPVFCVSIAL
                     ESDGNVVLGVIYDPMRDDLFSAVRGKGAYLNNKKLSVSSIRDISRSLLATGFPYDIRE
                     SRDNNLDYFNRMAVKVQAIRRAGAAALDLAYLAAGRFDGFWELKLKPWDTAAGCLMVE
                     EAGGRISDLSGKKWDIASPGVLASNGLVHKKMLDVLRMKPLGG"
     gene            complement(19961..21391)
                     /gene="pyk"
                     /locus_tag="PLG98_06220"
     CDS             complement(19961..21391)
                     /gene="pyk"
                     /locus_tag="PLG98_06220"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOE32887.1"
                     /translation="MIKKRKTKLVITMGPALQDRYLLREALKLADAVRLNASHSRPEE
                     RTPILKLIRNISDDLGRIIPVFLDLQGPKWRIGLLEAPVNLEKDSIGVLYLAGTPAPE
                     GYPWVAPLPHQELFTGAKVGQIWVLDDGALKLDVMETHHQQIMLKVLVGGMLKARKGV
                     HPIGLDVAFDPLTPQDLEDVRWGVQEGVDLFAQSFVRRASDVEALNLHIKELGGPQTI
                     IAKIEHPQALDNLEEILHVSWGVMVARGDLGVEFGVEKVPALQKQIIRKARQALKPVI
                     TATQMLESMIENPQPTRAEASDVANAIWDGTDAVMLSAESAVGKHPLEAVRYLHSIAA
                     DADAHYKPRIGLLADTLEENLSSRTDVSVAFAACRTAEEIGAKLIVVFTEGGGSARLV
                     SRLAADIPVIGATTDIGNSRRMGLLRGVESLLIPRAKHLSEMLASIQPLLKIRKGLES
                     GDRVVMTLGHPLWTTGTTNMMRVETY"
     gene            complement(21519..21812)
                     /locus_tag="PLG98_06225"
     CDS             complement(21519..21812)
                     /locus_tag="PLG98_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE32888.1"
                     /translation="MTKIREAAEKGIAYSQYVLGEMYYKGKGIPQDSDEAVKWFRKAA
                     KQGEVYAQYNLGWIYGTGTGVPQDYAEARKWYRRAAEQGNASAQLRLNEMKDK"
     gene            complement(22417..22602)
                     /locus_tag="PLG98_06230"
     CDS             complement(22417..22602)
                     /locus_tag="PLG98_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32889.1"
                     /translation="MGKGLKGFRDYMDYLTYAEIYLFWIDGNEHKKTYNMLMKRQGDI
                     EPIRYMRKMSLAAHSQN"
     gene            complement(23077..23472)
                     /locus_tag="PLG98_06235"
     CDS             complement(23077..23472)
                     /locus_tag="PLG98_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE32890.1"
                     /translation="MKEISLKTLSRVEMIDITASVQEAVRDEGVQSGVCLVYTPHTTA
                     AITINENADPDVQRDMIAALDRAVPLSANYRHAEGNSAAHVKSSLVGASELVIIENGH
                     LVLGTWQSVFFCEFDGPRTRKVFISMIAG"
     gene            23784..24059
                     /locus_tag="PLG98_06240"
     CDS             23784..24059
                     /locus_tag="PLG98_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32891.1"
                     /translation="MKKTLGTMIVAAAVILFTATFSFAEYAAAGAANFPFFQLGCLIV
                     GGLIMVSLKRKYDKMYVGEVVGIFALYTILMALFTNPVIETVKTIVS"
     gene            24314..24655
                     /locus_tag="PLG98_06245"
     CDS             24314..24655
                     /locus_tag="PLG98_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32892.1"
                     /translation="MSDGLRKIAFIVMLLAALGWIAGCAAGSQALLAEDYKAMDNENL
                     LRYYYRLNDEIERQEKRSGPQFSVGIGGFGGSIGGGAEVGAGGTRYTAHDLRARRIDA
                     LMELKKRGVTP"
     gene            complement(24722..24798)
                     /locus_tag="PLG98_06250"
     tRNA            complement(24722..24798)
                     /locus_tag="PLG98_06250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24762..24764),aa:Arg,seq:acg)
BASE COUNT         6242 a         6163 c         6311 g         6378 t
ORIGIN      
        1 cgtcgtttcc gcccaatacc taccggctga atccatacaa cggatggcat ccggatatgc
       61 gcgaactgga acagaaggtg agaagccaca aagccattgt cggcattctg gtcatcaacc
      121 cggacaatcc caccggcttt gtctatcctg aagaaacgtt gcgaaatatt gtacggatcg
      181 ccaaagaaaa cgatcttttc gttgtctttg atgaaaccta catcaatatt gtttacaacg
      241 gcaagaacac cgtaccgctt tcggatatcg taggctccgt tccggccatc agcatgaaag
      301 gaatttccaa ggaacttccc tggcccggct cacggtgcgg ctggatggaa gtttacaatg
      361 ccggtaaaga tccgatcttc gaccgcttta tcaataccat cctccatcag aaaatgtcgg
      421 aggtttgttc gacaacattg ccgcaactgg cgattcctaa aatcatgacg catccggagt
      481 atgataaata cctccaggaa cgcaggaggc attacgaaaa gctttcggat atcgcctaca
      541 acattttaaa agatgttccc tgcatgatgg tcaataaaac agacggcgct ttttatatga
      601 caatcgtgtt caacgaatcc ggtctcaaca gcaaccaaaa gcttcatata aaaaatggag
      661 agataaggga attcgtcgaa aatctcgtca aagaacccat cgaacacgac aagagatttg
      721 tttattatct gctggcggcg acggggatct gtgttgttcc gctgacatcg ttctttacac
      781 cgcttttagg cttccgcatg acgcttctgg acaaagatgt ggatgaattc gaacacacgg
      841 tcaaaaccat cgccgaaaaa ttcaccgaat atattgattc ttcaagcaaa gggatgcaga
      901 ccgccatcaa attatattaa ggagtgaccg cgagacaagc cttttaattt gatccggcgg
      961 aatctgcaat gacgggccgg gcagttttgc gtgattgcag ttcaggggta tccgatgacg
     1021 ctttcggttt tgttgttcat tgcgggcatt gtgcttttat gggcgggcac ggaattcgtc
     1081 ctgcaacgcg ttcccattct ggcggggtgg ctgcgggtat cgccgctggt ggtgaccgtt
     1141 ctgttgctgg cggtgatgac gtctctgccc gagttctgcg tttcactgtt tgcctccatg
     1201 cgcggacagc ctgccgctgc ggtcggcaat gttgtcggct ccaatttcgt gaccctgacg
     1261 tttgtggccg gcatttgcgc cctgtggaga ccgatcatcg tcggtccgac ccttcgtgaa
     1321 agagaatcca gctggatgat tctttccgcc gctctgttgc tggtcattgc catggacgga
     1381 gtcatcagcc gcatcgacgg cctgattctg ctgatggcat acggtccgta tttgaagggc
     1441 accatcgatg aggcgcgtca gcagagaaag gaagctcccg ttgtcagcat ggcaaagccc
     1501 tggctgcacg gcattatatt tgtggcgggc attgcgatga tcgtgctggg gtcgagctgg
     1561 attgtggaaa atggaacaat gcttgcgcgg cggctgggca tgaacgatct tctcatcggg
     1621 gtgacctttt atgcgtttgg cacatcgctg ccggaactcg ccatcgcctt gggcgcggta
     1681 ttgaggcgtc aggccgatgt gacgctcggc gagatctacg catccaacat ctttaccgga
     1741 ttggccgtca tcggttgtct gtgcctgatc aagcctcttc cggtcgaacc gatcatcgtg
     1801 cagcgcgatc ttcccatgct gatcgtggcg ggcgttcttc tgcagatgtt tgtcaccacc
     1861 ggaagaaaat ttgttcgtct ggaggcgctg gtgatgattg ccatctacgt gctttttctc
     1921 gccgcgcagt tcatgggttt cagcatatct ctgtcctgat caagtttcag gctgacgtcc
     1981 gattcggata atgtcgggac atctgatcat ccgcgatgct taagctgcaa tccattaaga
     2041 attgtgagcg ttgtgcaaat ccgcttaaga agcaatccgg tgaattattc cacctctttc
     2101 agagccagat cctgataggc gtggaaaaac ctgtggcaaa acgcatccca tcgccggcag
     2161 atatccatgg ttttttcatc cggctggaaa agattggggt acgccaccag ttcggtgatc
     2221 tcgtcggcca attctttaat cgttgactca ttcaagccgg aaagaatatg acgcacaaac
     2281 tccgcaggct tataccagcc gctgactttt tgcaggtcgc gatgaatctg tgtcaaaaga
     2341 acatttcgcg gaccttccca cagttcgttg atggcggagt cgcggtacag acgaggcagg
     2401 gatgagaaat cttccatcac tccgtggccg ccgaaaatgg acatggactc gcgaatgaca
     2461 tccgtgcagt cccaggcggc ggccatcttc tgcagcatga tgagttcccg gatattgaaa
     2521 cgattcttcc ggaccgcttc cggctcatcc gtcgccagtc cgcctttgag tcctccttcc
     2581 agcgtcagaa aatcccgata aagtttaaac gctccagctg ttgtcctttt ggccatgcga
     2641 tcgattctgt tcacctgaac ggcgaccagc gggaattggc cgatggtcag gccgaaggca
     2701 ttgcggaatt cggtgtattt cttcgcttcc cggacggccc gtgtcatgta ggcggcggcg
     2761 gaaagcccca cggtcaggcg cgagtaggtc aggacaatgc cgaccacatt ggcaacgcct
     2821 ttgtccagag gacccactgg ataagcgact gcgccgttga acgtcagttc cgccgtggtc
     2881 agttcgctgg tgcccatttt ccatttgatc cggtcgacgg tgtagccgtt tcgcatttct
     2941 ttttccttat tgcccggaag ccaggaaggc acgacaaaga gggcgacttt ttccgatccg
     3001 acgggttttg ccgtcaccac ggcatagtcc gcatgtgtgg ccgagcagaa aaattttgtc
     3061 ccatagagtt tccacgtgcc gttttcattg acagcttcca gcacatttgc cggcacatcc
     3121 gaaccgccct ggatttccga aagatactgg gcgccgatgg caaaatcgcc gttgatgccg
     3181 tctttacaat gtttgagaat ttctttcgtc tccggcgagt cggcatattt ttcaagaagc
     3241 attaccagcc cctctgtgca ggtgacaggg cagccgatac aggcttcgcc gttctggtag
     3301 atgacgtaca ttttaattaa tttgacccag ggcgacgttg tgtcggcaaa gaatgcttcg
     3361 gaaaaaattt ccttttccat gatttccgtt tcatgcgggc ggacgattct gtcgatgcgg
     3421 ttatgatgcc cgtcgtagtg cagcatgaag ggcctctttt cgggccgggc aatgctgtcg
     3481 gcaaaatcgc gccaccggaa agaggctttt tgagagcagg cgcgcgcttc ggcgtcaacc
     3541 tgctgccgga tgtctccggt aaaatggtgt accaccttct ggataaacgg atcatcccgg
     3601 tagtagtcaa cgccttgccg ccactttaaa aaatcatcaa acgtataggg attgttgcga
     3661 tcagatagag acatataacc tcccggataa tcttatttgt gattgacttt ctcaaccaac
     3721 ggttatatta caaaccactg gttacataag tcaagctttt ttactgcaat gtttttgcgg
     3781 gatatggtat aaaaaaatcg atatgaaaaa gaaaaatact tttgccgaac tgaaaaataa
     3841 cgaaaaacgg gcgcggcaag gcgttatcgt cgaagctgcc cagcgcgtct ttgccgtaaa
     3901 accgttcaac aaagtcagca ttcgcgacat tgcccgggag gccggcattt cccacgcctc
     3961 catctaccgg tatttccccg atcagcaatc cctttttgtg gaagcctttc ttcacggcgc
     4021 ggaagaaatt acggccattc tggacaggat gatcaaaaac agcagaaaaa acccgatcga
     4081 aaaggtggcc gatgcctatt tgagttatct catcgataac gatcagtatt tccgcatgat
     4141 gacgcgtttc atgctggacg gttcgctctc cgcggacatg gtggaaaagc tcaacgcctc
     4201 tgaacgccgt atctttgatc agttcgacgt tttgttcaga aaaatgaacc tgccggaacc
     4261 ggtcaggcct ctgtcccatg cattttttgc tgcgctcaac ggtgtgctga ttactttcag
     4321 aaactatccc ggccgaagcg caaaagccgt gcatcagcat atgcataata ttgtccgggt
     4381 cattgtatcc aagttttgcg taccttctgc atagctccct tcatgggcac accaaaaaaa
     4441 gagcagaagg tttcaaaatg ccttctgctc ctctggattt ttattttgcg cctctggccg
     4501 tcccgatgtg tctagaatgc gggatcaggt ccaaagctgc ttgttttatg cgttttcaac
     4561 gcgagaatga ttaatatctt ccatccctgc ggccgccccg gttgaatcct cctcctccgc
     4621 cgcggttgcc gcctccccga tggccgccgc tgaactcttt cttcggtctg gcttcgttga
     4681 ccgttatttc ttttccgtca agcgtgccgc cgtggaattt tttaattgct tcggctgcgc
     4741 cttcttccgt tttcatctcg acaaaaccga atcctttgga ctggccgctg aatttatcct
     4801 tgatgatcgt cgcggagacc acttctcccg cttcggaaaa attggatttc aggtcatcgc
     4861 ctgtgatctt ataagacaga ttgccgacgt ataaattctt gttcatctct ttatcccctt
     4921 tctgatttta tttctttttt tcattccccg atggggtgtt acaaagcatc catgtcaccc
     4981 ccatccgtct cctggttgag aaggaaggaa aaagtgaggg gcttgtctgc cttacatctt
     5041 taaagaaaaa accatcagga catgaataat ccttgatggt tgtaatccgt ttgtaaaaaa
     5101 tatacggaat cgccgccatt aagttgcgca tactcagaat ttttagtcaa gcagtatttc
     5161 gttttttcct ggcaaccatt atttgatcag ttttttgcgc atgacggaaa gggcgagcgg
     5221 aaaggtgatg agacagaaaa taatcaggta gcaaaaaccg gccagcgctt cggcgttgaa
     5281 cgctcccagg cctaaggccc gcaccaggtt gaccagatgc gtcaacggca gcgtcaaggc
     5341 gaaatattgc gcccagaggg gcaatccgct tatcgggaaa aacgtgccgc tgaaaaggaa
     5401 catcggggta atgaacagaa agaccggcaa gttgaataat tcgatcttgc cgaccaggcc
     5461 ggtgaagatc atggccacgg agccgaaggc aaagccgcca atgaaggcga caagaacgat
     5521 cagcaatcct gccggatagc tgatcaatcc gaagaaaccg atgacaatca gcatgatgat
     5581 cgttccgatg accgatttgg tcgcccccca gacgatttct ccggtcacga tttcgtcaac
     5641 cgtgagcggc gtggccatca tcgcatcaaa cgttttctga tagtacatgc gcacaaagga
     5701 tccaaaagtg ttttcaaaga aagagttgtt catgatgttg attgccagca gcgcgggagc
     5761 gataaacatc acgtaggaaa tttccgttcc ggcgtacgtc accgatccga tcagtccgct
     5821 gaaaccgatg ccgaacgcca aaaggtagaa aagcggttcc agcagcggcg gcatgaagtt
     5881 gatcttccag ctttctcggt agaccgtcag gttgcgctgc cagacacgga tgaatcgggt
     5941 ggaaattctg aaggtcattc ccgcagctcc cttccggtca gtttcaaaaa aacatcttcc
     6001 aacgtggcgg aacgcaggat gcagcttttc tggctgaaac ggtcgcagag ctcctgatag
     6061 agttcatggc cgttggcggc gtaaataatt aaacgatgtc ccaggtcctc cacccggtcg
     6121 tgcttttctt gggaaaacgc gcgcaatgct tcatccggtt cggcaacctc aatgatatgg
     6181 ccgcccgtgt attcccggat gagcgaccgg ggagagccct gcgcgagaat ttttccgtga
     6241 tccataatga ccaggttatc acacagacgc gccgcctcat ccatgtagtg cgtggtcagc
     6301 aggatggtca tgccctgatt cttgagttgt tccagtcttt cccagacctg atgacgcgac
     6361 tgaggatcaa ggcccgtcgt cggttcatcc aggatgatga gatccggctg attgatcagg
     6421 gcgcgggcga tgaccaggcg gcgcaccatg ccgccggaca attccgtggc gcgggcattt
     6481 tggcgttgtt ccagcgcgaa aaatttcagt agcgttgtcg cccgggcgag ggcagttttt
     6541 ttcccgatat caaagtagcg ggcgaacact tcgaaattct gcatgaccgt aagatcgggg
     6601 tcgagcgtat tttcctgctg gcagacgccg atgcgggctt tgattttccg gtcatgtgtt
     6661 ttgatgttca gtccgaaaac aaaaatgtcg cctgcggtca gcggcgagaa accgtaaagc
     6721 atccggatgg tggaggtctt gccggcgccg ttcggtccca aaatgccgaa acagactccc
     6781 ggaaccacat ggaaggaaat atcatcaacg gccttgaacg cgccgaaaac tttttccaga
     6841 ttgcgggcga cgatgaccgg ttcgatcgga cgggtttcaa tcataggctg ttcaccataa
     6901 agcacacaaa aacttatgtt aatcatccaa gtgttgtcaa caaaaagaaa tccctcattt
     6961 ttgtgtttga cgaaacaggg cccttctatt atagttgtaa aggtttatat tcagcaactg
     7021 acgatgcaac aatgaaattt tatcttttaa cgggggttca aaccatgagt aaatcaatgg
     7081 acaaagggaa aaaagacgcc aagaaaaagc cggcgaagtc cgccaaagaa aaaaaagaag
     7141 ccaaaaagtt gaaaaaagcg gcgaaagccg gaatgtaaga taaacacaaa cgacaaatct
     7201 ttacgggtgt cgactttctt tacaaagcgc actttatccc gcctccatgc gggataaagg
     7261 catttttatt ttccgggaag gtcacccgct acggatttta cctcgaaaga actgacgaaa
     7321 aggatttccc catgaaagtt aaatttctcg gagcggccag agaagtcacc ggctcctgtt
     7381 ttattatcga aaccgatcag gcgcgattcg ccgttgactg cgggatgcat cagggcggtg
     7441 cggatgttga aacacgcaac tgggacactg aagcctacga cccgtctcat atcgatttct
     7501 ttctgatcac gcatgctcac attgatcatt cgggattatt gccccgcatg gtgcagaacg
     7561 gattcaaagg gcctgtttac gcaacggagc cgacggggga actgatcaag atactgcttc
     7621 tggacagcgc ccatatccag gaaaccgatg caaaatcgaa gacgagaaga atgcagcgta
     7681 acggcaggac ggagaaaagc gagccgcttt attccacgcc ggacgccgaa gccgtgtccc
     7741 ctttgatcaa gaaaataacc tacaatcaga attttacgcc tgcggccggc atcaaaatca
     7801 attttcagga cgccggccat attttggggg cggccatcct ggaattgttt attgaggaaa
     7861 acggcacctc cacaaaactt gttttctccg gcgatatcgg ccgccgtcat cagttgctca
     7921 tggaggatcc cgttacgatc agcgacgcgg actacctttt tatggaatcc acttacggtg
     7981 atcgcgatca taaaggcgaa gaggacagtc tcaatgaaat ggccgaggcc attcagtaca
     8041 gttattccag aggtgaaaag atcatcattc ccgcttttgc cgttgagaga acgcaggaga
     8101 tgctgtattc cctttatctg ctggacatca aggggaagct gcccaaggat atgccggtgt
     8161 ttctcgacag cccgctggcc atcaaggcca cagagatatt tcggcggcac cacacatacc
     8221 tggatgcgga tacgcaaaac atactgaaaa atggtgaaga tcctctggat ctgccgcagt
     8281 taaagttttc gtcctcgacc gagcaatcaa tgagaatcaa cgaaatggaa gggtcggcca
     8341 ttgtgatttc cgcatcgggc atgtgcaatg ccggacggat caggcatcat ctacggcata
     8401 atttgtggcg gcccggcgca agcattgtgt tcgtcggatt ccaggcgcag ggaacgcccg
     8461 gccgtaagat tgttgagggc gcgaaaaaaa tccgtatttt taacgaggat atcgccgtca
     8521 aagccaaaat atggaccatc ggcggttttt ccgcgcacgc cgggcaacgt cagcttctgg
     8581 actggctggg acattttcag aataaaaaaa tgccggtgta tttagtccac ggtgaagccg
     8641 gtgcgcagga tacgcttgcc tcgttgatcc gcgaacggct gggatttgag gttgccattc
     8701 ccgagtatct ggaagaaatc aatattaaag cagggacccg gccgcaggaa attaaacaac
     8761 cgcgtccgtc cagaccgccg ctggagcttg ctccgctgct ggccgatctg aaagcgaaga
     8821 tcgattacat caacgcccgg gtggacaaaa ttcaatccct tcccgaatcc gagcagcagg
     8881 aagtgattca actgctcaga caggtcaatc aaaacatgga tgacataaag cctgatttgc
     8941 agacgggatg aaagcggaat ttttcccgtt gtccggggtt ttggaacgcg gctaatccat
     9001 gatcttgtag ttaaaagaag caacgatatg cagccgtgac aaattgtatg atcccggcaa
     9061 aggttcgtag ggcgcggctt cctgaacgac gcccagcgcc ccgttatcga gatcggctga
     9121 tcccgacgat gacacgagcg ttacggcggc cagtctgccg tccgcatcaa tggacattct
     9181 gacgacaaca ttcccctctt catttcgttc gaaggacgtc tgcgggtatt tccatatccg
     9241 gaggatcttg ttttttaccc gggtcaggta atgaatgtat ttcgtatcga gactgcccag
     9301 gttaattgta tcttcgcgcc aggcatcatc gggcgtaggg tcgttctgtt gcttcgtcac
     9361 gctggacgac gggtttcctt gggcagggtt ttctttttca ggagaaggtt gcgccggcgc
     9421 cggttcttgt atgctgaccg acagcacgtt taccggtttg attttatcgt ttaaatcgat
     9481 catgcctgcc acggccagta gcgtcatgtg gatgacgacg gatatgatca ccggccacaa
     9541 aatggctttt ttatccatgt caacgtttcc cgaatctgaa agtcttttgg agcctgtgtg
     9601 tcccaggcgc cctgcactat acagtatttt tttcaaaaac cataatatga atttaaaatg
     9661 aaaagacaat tccgtgcgcg tcgctaccgg cggtacggcg aaaggttgcg gcagaagaaa
     9721 aaggaggggc ataaaaaaga caggcaggat agaacctgcc tgtctttcaa tgtttttgtc
     9781 tactcaacat tggcactgac aacagaggca atctgcatca tgtcaatcgt ttgtccttct
     9841 tttaattttg tgaaatccac gaccttgccg ctcgatgttt tcaccttggt gttgttgatg
     9901 atcttgatca gtttggcgtc gatcacaatg ttctttccgg cattttccgg tttgccgttg
     9961 acttttgtgg tctgcagttt attggcttta atgtaatccc aaagtttttt ggtaatctcc
    10021 gtgcggggaa gtgctttggc gcccaaaagc tccgcgagat cccccttcag cttgaccggt
    10081 ttgctcaatc ctttcttttc tgccatactc attgcctccg tttttgaatt ttgttggtat
    10141 tgttgataag ttactgtgtg gcggagtata agaactttct gttttgtatg tcaagaaata
    10201 ttccgagaag atatttgatc cccggacgtg catgtcccgc gtcaacgcaa aggggaaacc
    10261 tgttgaatat tatattgaca cgcaatcatt cttcttatag gtcaaccccg taaaccaaaa
    10321 cgattcggtc atcgattcag gagaaaaaca atggcaaacc ggtcaacgca aaaacgcgct
    10381 gtaaaaattc cccggggcaa ggcgctgctt tgcgatcccg gtttaaacaa agggacgatg
    10441 ttcagtgaca cggagcgcga cgtcctgggg ttgcgtggtt tgcttccgcc ccgtgttttt
    10501 tcgccgtccg accggacggt ccatatcatc aacaatgtcc ggcgcaaaac aaccgatctg
    10561 gaaaaatatc tttacctttc cggtctgcaa aaccgcaacg agcgtctttt ctaccgggtg
    10621 ttgattgagc acatacagga attgatgccc gtgatttata cgccgaccgt cgggcaggcc
    10681 tgccaggagt acagcagcat tttcagcgaa cctcgcggac tgttcatttc caaaaatgac
    10741 cgcggcagca taaaaaagat cctgaaacgc tggccgcaga aaaaggtccg ggtcattgtt
    10801 gttaccgacg gtgaacgcat cctgggcttg ggcgatctgg gcgccaacgg gatgggaatt
    10861 cccgtcggca aactggcgct ttacgccgcc tgcgcgggta ttgatccgga agcctgcctg
    10921 ccggttaccc ttgatgtcgg taccaacaat ccggagttgc tggctgcgcc ggactacatc
    10981 ggtctgccgc agccccgggt gcgcggacgg gaatacgatc agttggtcga cgaattcatg
    11041 gccgccgcgg cgaacgtttt tcccggcgtg atgattcaga tggaagattt cggcaatgcc
    11101 aacgctttcc gtctgctcga aaaatatcgc agcaaatact gtatgttcaa cgacgatatc
    11161 cagggcacgg cgtccgttgc gctggcgggt ctgtatgccg ccctgcgtat aaccggcggc
    11221 agtatttccc ggcagcgact gctctttttg ggcgccgggg aagccgccat cggtatcgga
    11281 gatctggttt cttcggccat gatggaagaa ggcctgtcgc tgccggaagc gcattcaaac
    11341 tgctggttcg tggattccaa aggactggtt gtcaaaggcc gcaccgatct ggtggaacac
    11401 aaaaaaccgt acgcgcatga ttaccccttc catcgcgatc ttttgtccgc ggtggaagcc
    11461 ctgaagccga ccgcggtcat cggggtatcg ggtcagcccg gaacctttac accggaaatt
    11521 atcaaggcga tgggccggat aaacaggcgg ccgatcattt ttgccctgtc gaatccgacc
    11581 tccaaggccg agtgtaccgc cgaagcggct tatcgtcaca ccgaagggag ggcgattttt
    11641 gccagcggca gtccttttgg cgaagttagc atggagggga aaacgtttgt ccccggccag
    11701 gccaacaacg cgtacatttt tccgggagtc ggactgggtg tgctgacctc cgaagccgtg
    11761 cgcgtgacgg atgagatgtt tacagccgcc gccaaatccc tgaacgaaca ggtgtctgct
    11821 gacgatctga aaatcggccg catctatccc tcgttgacaa caattcgcga ggtatccgcc
    11881 aaaattgcac tggccgtggc cgaagtcgtt tttaaacgtt cgctgacccg gatgcaggcg
    11941 cctgctgatc tgcgcggaaa tatccgggca acgatgtatg aaccggactt cggaacatat
    12001 atttaggtcg ataacacttt atcgtgtaaa ggagcatagg gtatgaaaaa aaaggataag
    12061 cgttttgtag gatcgttggc gtttgttttt gcgctgttgt ttgtcataag ctcagccggt
    12121 gccgccctgg cggatgccgt atcggccaaa atagggtttg tggatatcgc gaaaatcatg
    12181 agagactcca gagccgccca gaacgcccgg gctgtctatc agaaggattt tgagtccaaa
    12241 aaagcgacac tcaaagcgaa gggcgacaga gtttcctctc tggaaaaaga tttgagcaac
    12301 acgaaacagg attctccggc tctcaaggaa aaacgggaga agctggcgca ggaattcaag
    12361 gagttcaaac ggctggaaag cgatctcaat gaacaattga agaagaaaga tgccgaactg
    12421 acccgaaaaa ttctcatgga cgttcaggag attctgaaac aattttccaa aaacgaaaaa
    12481 tactccatga ttttcgaaaa gaaagcggtt ctggttgttg acgacggctt tgatgtgacg
    12541 gacaaaatca tcaaaattta tgacgcgcag aaaaaataat ttgtcgcacc gattgccata
    12601 acgaatttgt catttatgca ggcgcaccca taacagtgcg cctgcatttc tttagcaata
    12661 tcctctctat tttgtgcatg aaccgtgcgt aagcgatgtc ctcttccgca aagcagatac
    12721 ccccggcttg cgggtgcgga attgaggtta aaacatcaaa tcataagtgc gtcgaagcct
    12781 tattgatcgg cgcggggcgt gatcgcgcaa agcggatcgc aaccgatccc cacggcgcac
    12841 gttggacatg atattagtta ttgacttaca aaattgttct acttatagtt caaaaatgaa
    12901 acgttttcgg gatagatcaa ttttatgtca ggagaaaaac tcagatgatt caacagcccg
    12961 gtttcgataa cgaaaaatac atccaggaac agaccaccga aatcctcaaa cgcgtcaacc
    13021 gcttcaacaa caaactgtat ctggaattcg gcggcaaact tttatacgac tatcatgccg
    13081 cccgcgtcct gccgggttat gatcccaacg tcaagatgcg cctcatccgg gaattaaaag
    13141 acaaggccga cattgtgctt tgcatttatg ccggggatat cgaaagaaaa aaaatccgcg
    13201 ccgatttcgg cattacctat gacgcagacg ctttaaagct tattgacaac ctgcgaacct
    13261 ggggaatcaa tgtgctgggc gtggtcatta cccgttttga agaacaaccc gccgcgatca
    13321 tctttaaaaa caaactggaa agacgcggta tttccgttta tacgcaccgc tttaccaaag
    13381 gctatccgac ggatgtggag accattgtca gcgatgaagg atacggcgca aatacctata
    13441 tcgaaacaga caaaccgctg gtgattgtga ccggtccggg gccgggcagc ggaaaaatgg
    13501 caacctgtct ttcgcagctt tatcacgatt accggcgcgg cattaaatcc ggttacgcca
    13561 aatttgaaac atttcccatc tggaacattc cattaaagca tcctgtgaat gtggcctatg
    13621 aggcggccac cgccgatatc cgggatttca atctgatcga tccgtttcat cttgacgcgt
    13681 acagcgaaaa atccgtcaat tataaccgcg acgtggaagt ttttcccgtc ctgaaaagaa
    13741 ttctggaaaa aattacgggc gaaccgtctt tttacaaatc gccgacggat atgggggtca
    13801 accgcgccgg gtttgccatt atcgatgacg cgatcacgca aaaggcggcc aagatggaaa
    13861 ttatccgtcg ttatttccgt taccgctgtg aatacgccat gggattcgcc gatcgcgaaa
    13921 ccgtgcagcg cgtggaactt ttcatgaagg attttaatct ggagccggag gagcgtccgg
    13981 ttgttcaacc ggcgcgcgag gctgccgtaa ccgctcccgt ggaaacgcgg gattacgaac
    14041 gcggaaacga gggcatcttc tgcggcgcag cgctggaatt gaagaacggc aagatcatca
    14101 ccggcagcaa ctcatcactg atgcacgctg ccaccagcat ggtgttgcat gccatcaagc
    14161 atctggcgga aattccggat aagattaaat tgctgccgcc ccagatcacc gattcggtca
    14221 aaaatctgaa aacggagatt ctcgatgaaa aatcggtgag ccttgatctg gaggaagctc
    14281 tgatcgctat cagcatcagc gcgacaaata atcccgccgc gcaactggcg ctggaaaaac
    14341 tcagggagct gagggactgc gaagcgcaca tgacgcacat tcccacaccc ggcgatgaag
    14401 cagggttgcg ccgcctcggc atcaacctga caacggatcc gaatttttcc actaacgatt
    14461 tattcattac ataaaataat accgaccggc tgcgaatcgt aactatcctt tttctttaga
    14521 gaatcatctc gaatgccatg ctcttttcgc ttgcggcttc ggcaaaacag gttgacaaag
    14581 cccccccttt taattaataa tttttgagac atgaagggag gtcatactat gtcagtgaag
    14641 attgccatta acggttttgg aagaatcggg cggcttgttt tcagggccgg atatcaaaac
    14701 aaagatgttg actttgttgc ggtaaacgat cttgccgacg ccaaaacgct ggcgcacctt
    14761 ttgaaatatg attccacaca cgggatactc gatgccgaca ttagcgtcgc aaacaacgcg
    14821 attatcgtag acggaaaaga actgaaaact ttttccgtcc gagaaccgga gacgctgccc
    14881 tggaaggatc tcggcgttga tgttgttctg gaaagcacgg ggaaattcac cgataaaacc
    14941 ggcgccggaa aacacatgga ggcaggggca aaaaaagttg tcgtgtcggc gcctgctaaa
    15001 aatcctgacg tgacgtttgt tctgggggtc aatcaggaag tatacgataa aaacaagcat
    15061 catatcattt cgatggggtc ctgtaccacg aactgccttg cgccgatcgt caaaatactg
    15121 catcaggaat tcggcatcga atacggtctg atgacgacca ttcattcttt cacaaatgat
    15181 caggttgtgg tggatgaacc tcacagagat ttacgaagag cacgcgcggc ggctctttcc
    15241 atgataccca ccaccaccgg tgcggcccgg gccatagcgg aagtcattcc tgagatgaag
    15301 ggaaaactcg acggtctggc cattcgcgtg ccgacgccga atgtttcgct tgtggatttt
    15361 gtggcaacac tttccaaaag taccacaaga aatgaaatca atgataaatt ccgtgaatat
    15421 gcccaaagcg ccatgaaggg tatccttgcg tgttcggaag aagagctggt ttccaaagat
    15481 ttcaacggca acaagcattc atccattgtg gatatgccca acacgacggt tatcggcggc
    15541 aacatggtca agattctctc ctggtatgat aatgaatggg gcttttccaa cagaatgctg
    15601 gaactgctct cgtttataat gaaataggga aggtgttcaa gatgaagtac attgatcaga
    15661 tcgacatgaa aggcaaaaga gtttttttac gggtggattt caatgtcccc atggataaag
    15721 aaggaaacgt gaccagcgac aaacgcgtgc gagccagtat ccccaccatc acctacgcgt
    15781 tggaaaaggg tgcgaaattg atcgtcgcct cccatctcgg caggccgaaa ggcaaaagag
    15841 tcgccgagat gtcactcaag cctgtcgtca aggttctttc ggatcttctg aagaaagagg
    15901 tcctcttcct ggaggattgc gtgggaccgg ttgtggagca gggtgtggcc aggcttcagg
    15961 atggccagat tatccttttg gagaatctcc ggtttcagcc gggcgaagat aaaaatgatg
    16021 ctcaattcgc caaacaactg gcggcgcttt gcgacgtcta tatcgacgac gccttcgccg
    16081 tatcgcatcg ggcggcggct tccaattcgg caattaccga atttgttccc gtatgtgccg
    16141 ccggttttct gttgaagaac gaagttgaat atttcaaaaa agccatggtg aatccggcca
    16201 aaccgctggc ggcgatcatc ggcggggcaa aggtttccga caaaatcggt ctgttggaaa
    16261 acctgatcga aaaagtggac aaaatcatca tcggcggcgg aatggccttt acctttttga
    16321 gagcggcggg atatgaaacc ggcaagtcgc tctgcgagga agatatgctg gataccgcgc
    16381 gaaagatcgt ggacaaggcg aaacagaaga acgtacagtt tctgctgccg gtggatgccg
    16441 tcattgcaca ggccgccgag gctcaagccg aagccaggat ctgcggtgtg caggatattc
    16501 ccaaagaatg gatcggcctg gatatcggtc cggcaaccat cgcattgttc agcgaggcct
    16561 tgaaaggcgt aaagacggtc atctggaatg gtcccatggg gatgtttgaa cttgcccctt
    16621 tcagcaaagg aacgtttcag ctggctcagg cggtggcgga ttccggagcg ctatccatcc
    16681 tcggcggcgg tgacacggat accgccatta agaaagccgg tctgacgtcg aaaatgtctt
    16741 atatctccac cggcggcggc gcgtttttgg aatggctgga aggcaaaacg ctgcccggca
    16801 ttgctgcatt ggaaaaataa cggttatcag gacccaaaca agatcgtgag gacataacga
    16861 tgagaaaatg gattgttgcc ggcaactgga aaatgcataa cacgattgcc gaatccatca
    16921 tgcttgcgaa agccattcaa gacggatcat cagacattca gggcggagct gtcatcctgg
    16981 ctccgccttt taccgcgctt tccgctgtgg gcgaggcgat caaaggctcc tgtctggaac
    17041 tggcggcgca aaacatgcat tatgaagaca agggtgcatt taccggggaa gtgtccccgt
    17101 tgatgctcaa agatatcggc tgccggtatg tcatcatcgg tcattccgaa cgccggaaat
    17161 attttcatga gcagaatgcc gatgtcaatt taaaagtgaa caaggccttg tccgacgggc
    17221 tcatcccgat catgtgcgtg ggagagaccg atgaagaaag agaaaagggc attacacggg
    17281 atgtggtcgg caggcaggtt aatcacggcc tgaaaggtgt tgaaaaaatc gaccctattg
    17341 tgatcgcgta cgaaccggtc tgggccatcg gaaccggaaa agtggccacg cccgcgcagg
    17401 cccaggacgt ccattccttt atccgcggta ttttaaagga gtcctacggc gacgtggccg
    17461 gaaaaatcag catcctctac ggcggcagcg ttacccggga caatatcggc gatttaatcg
    17521 ccatggagga tatcgacggt gcgctggtcg gcggagcgtc attaaaggcc gagggttttc
    17581 tgggcatcat taagaccgtt gccggtggaa gataattccg ggataaacgt cgcaagcttg
    17641 ccgcgtgccg gaaacgcatt gtttgacgcc gttcgtccgc atcttgcgcg ataaaaacac
    17701 catggtttgc gcactggaaa aaatgaatgc aaaagccggg aaggatatct gcgcgagcat
    17761 cataaccgga tgaaaaacgg gttatgatgc ttatcgcgtc agcagttatt ttttatcttc
    17821 ttcctttttt gatgaattgt cggtgatttc gttttgctca ttgatggatt ttttgaagtt
    17881 cttgatgcct ctgcccagag ccgaaccgat ttccggcaat ttcccgacac caaaaataat
    17941 cacaacaatg attaaaacaa taacgagttc ctgccatccc attccgaaca taccctacct
    18001 cttgattttt attgtttgac ttgcggcaat cttatatgga tcacctctcc cggtttcccc
    18061 aggttctcta tagacctgcc tttgtattgt atcttaattc ctccggcatt gccaatatcc
    18121 agattaaaaa attcctcact gctttcaaat ttttctcccg gttttaacag aagttgaaca
    18181 ggcggactgt tgtccggttt tatgcgaagc cacgtttcct gaccggccgt gatcaataaa
    18241 acactttttt ttgcatcggt tgtcgccggc ggattgtccg tcacgccggt ttgcggcgcg
    18301 gggacagtcg cgggcgtctg gctgtcggtt aatgccgggg cggcaggaag cgttgcttcg
    18361 ggagcccgtg tggctgccgc cggcgtgttg atatcttcag ggacagactg attctgtttg
    18421 agaatcagcc agatgctgat aaaaatgatc aggacgagca agatccccca gtaatttttc
    18481 ctggcggcaa atgaggcaaa aaaagatttt gtctccggcg ggggctccgg cgggggcgtc
    18541 tgctcttcgc ggtgtgcatt caggtaggcg tcgtaatcct gcaaaacggg attttcgtcg
    18601 atgttgagaa accgcgcata ggttttaata aaattttttg tataaaccgg atccggcagc
    18661 tggtcaaact cgctattttc tatggcttca atataacggg ttctgatgcg tattttttta
    18721 aatacatcat caggcgaaag ccccagagac tctctcttca tcttcagttt ttgcgagttg
    18781 tcgctcggtg tctgttcgat aaacatggag attcacagat gaagccattt ttttgtttgt
    18841 gtattagcat taatttgatt gtaatacaac tatttaaaat aaaaacaatt attactttaa
    18901 tattttctga ttttattgtt agtatgccga agaaaacggg acaggagtat tttttatgcg
    18961 ggaattaaaa tcgtttgccg tcgagatggc gcgaaacgcc ggagcgctac tgaaaaagaa
    19021 attcaacaaa acccacaaga ttcaatacaa gggcgatatc aatattgtga cggaagcgga
    19081 caagatgtcc gagcatttga ttataaaatc catcagacgg aattttccgg aacatggaat
    19141 cttgtcggag gaatctccgg ccattgccgg cgccggaaaa aaacgctgga ttgttgatcc
    19201 tctggacgga accacaaatt acgcacacgg atatcctgtc ttttgcgttt ccattgcgct
    19261 ggaaagcgac ggcaacgtcg ttcttggcgt tatttatgac cccatgagag acgatttatt
    19321 ttctgctgtg cgcggcaagg gggcttatct gaacaacaag aaactgtcgg tttcctctat
    19381 ccgggatatt tcccgcagtc ttctggctac ggggttcccc tatgatatcc gggaaagccg
    19441 ggacaataat ctcgattatt tcaaccggat ggccgtcaag gtccaggcga ttcgccgcgc
    19501 cggggcggcg gcactggatc ttgcctatct ggccgccgga cgattcgacg gattctggga
    19561 actcaaactc aaaccctggg atacggcggc aggctgtctg atggtggagg aggcaggcgg
    19621 caggatttcc gatctgtccg gtaaaaaatg ggacatcgcg tcgccaggcg tgcttgcgtc
    19681 caacggcctg gttcataaaa agatgcttga tgttttaagg atgaagccgc tcggcggtta
    19741 agtttttaaa agcggttcgc gccgggcaac ccgtatatgg tcttgaatct caacggctgg
    19801 catttaatca cgcaaaacct ttatgatgac gttccgcgtc tgcggcccgt caaatttgca
    19861 gaaagaaata gattgccatg tgtccaaaac aagtccatgg cttgaccaat tagccttccc
    19921 tttgttttct tttgtgaaac acaagcaata agcactgata tcagtatgtt tccaccctca
    19981 tcatatttgt tgttcctgta gtccacagcg ggtggccgag cgtcatgacg actcgatcac
    20041 cggattcaag cccctttcta atcttaagga gcggttggat ggaggccaac atttccgaga
    20101 gatgctttgc ccgaggaatg agcaggctct ccacgcctcg aagaagaccc atccgccggg
    20161 aatttccgat gtccgtcgtg gcaccgatga cgggaatatc cgctgccaat cgggagacca
    20221 ggcgcgcgct gcctccacct tcggtgaaaa cgacaatgag cttcgcgccg atttcttctg
    20281 ccgtgcggca ggctgcgaac gctacggata cgtccgtgcg gctggagaga ttttcctcaa
    20341 gtgtgtcagc cagtagaccg atgcggggtt tgtaatgggc gtccgcgtct gcggcaatgc
    20401 tgtgcagata gcgcacggcc tcgaggggat gtttaccgac ggcgctttcg gcggagagca
    20461 tcacggcgtc ggtgccgtcc catatggcgt tggcgacgtc actcgcctca gcccgtgtgg
    20521 gttgtggatt ctcgatcatg ctctccagca tctgggtcgc ggtaataact ggtttcaaag
    20581 cctggcgtgc cttcctgatg atttgcttct ggagcgcggg caccttctcc acgccaaact
    20641 caacccccag atcgcctcgc gctaccatga cgccccagct cacgtgcaga atctcctcca
    20701 aattgtccag cgcctgagga tgctcgattt tggcgataat ggtttgcgga ccgcccagct
    20761 ccttgatgtg aagattcagc gcctccacgt cggaagcacg gcgcacgaaa ctctgcgcaa
    20821 acaaatccac gccctcctga acgccccagc ggacatcttc cagatcctga ggagtcaggg
    20881 gatcgaaggc tacgtccaat ccgatgggat ggacgccctt gcgggctttc agcataccgc
    20941 cgaccagcac cttcagcatg atttgctgat gatgtgtttc catgacgtca agtttcagtg
    21001 ccccgtcgtc cagcacccag atctgaccga ccttggcacc tgtgaacaat tcctgatgcg
    21061 gcagcggcgc cacccatgga taaccttcgg gcgctggtgt gccggctaag taaaggacgc
    21121 cgatgctgtc tttctccagg tttaccggag cttccagcag accgatgcgc catttgggtc
    21181 cctggagatc cagaaagact ggtattattc ttcccaggtc gtccgaaata tttctgatga
    21241 gttttaggat tggtgtgcgt tcctcgggcc tactgtgact ggcgttgagc cgaacggcgt
    21301 cggcgagttt cagggcttcc cgcaggagat atctgtcttg caaagctggt cccatagtga
    21361 tgaccagctt ggtcttgcgt tttttgatca taaattctct cctcctacta ttcttgtcag
    21421 gtctgaatgg tatttattag attgaaatgt tgtacgaatc ggaaaacgag aaaaacaatc
    21481 ataacgtata aagtatcggt tgggttccgc tacttcaatt atttatcttt catctcattc
    21541 agtctcaact gcgcacttgc gttaccttgt tcagccgccc ggcgatacca tttcctggcc
    21601 tcggcataat cctgtggaac gcctgtgccg gttccgtata tccagcccag attatactgg
    21661 gcatatactt caccttgttt tgccgccttg cgaaaccatt tcacggcctc atcagaatct
    21721 tgtggaatac ctttaccctt atagtacatt tcgcccaaaa catattgtga ataggcaatt
    21781 cctttttcag ctgcttcacg aatttttgtc aacgcgttac ctcacgggct aaatattact
    21841 tttggcatta caagatcaag gcttcctttt gggttgctta tcaaacttgc ttttttcctt
    21901 ctgtttctgt ttttcagtgt tctgcttatg acttttttct ttattttttt tgccgccttt
    21961 gtctcccatg atatgtcctc tatctttaat atttgtcttt aacttcacaa taggaagcct
    22021 tacccataat gtttataata gataatccga aaataacagc aataacatac ccgaaatttc
    22081 gacaagtttc catacccgcg tttacgccta aaaaatggaa catactattg aaaagcggac
    22141 ctgtttcaaa agcaatatta tacattttta attttccctt tctttaaaga aactctattt
    22201 aagggttaaa tgattccttc atatacatct tccatttgtg atttgggtca ctcacaaaag
    22261 tcctattctc cgggctgtca tattcatctg atttaaatgt cctgacaatc cataccccgg
    22321 cgactttata tcggtcactt ttgatatcac tgccgatagc tcttatggct tcccacattt
    22381 taactcatct ctcccgttgt ttatttgctc ccgaatttaa ttctgactgt gagccgccag
    22441 actcatttta cgcatataac gaataggttc tatatctccc tgccttttca tgagcatatt
    22501 gtaagttttt ttatgttcat tcccgtctat ccagaataaa tatatttcag cataagtcaa
    22561 ataatccatg taatctctga atccctttaa tcctttcccc atttttaatt cctcctggca
    22621 aaaaaaaccg agacccgcat atgccgtctt gtcattttac tggagtccat cattctaatt
    22681 ctatgtcttt tacgggtgat aatcaccaca aagactcttt tacttctttt ttgttaactt
    22741 ccagtagaat agactcaata cgggcatcgc agtatctcgt atctataata taagcatgac
    22801 tcatcatgaa tacaagagct gcgcctgtat tagtctgatg tgaactgtat ttgttacgga
    22861 tttaattctt gcctgtttgg acagttaaga ttggttgatt tcagtcaacc atcatgaagg
    22921 tagtttcgga caacaagttg ttcgctgtgt gaacatggtt taagtaatac ggatcaactt
    22981 atgcgcggtt gctaagatag ccgtcttgta aggcaaccct tcgttttttg ataggaagtt
    23041 ttaaaaatat cggtttattg aatcacaccg ttaaggttaa ccggcaatca ttgagataaa
    23101 aacttttctt gttctcgggc cgtcgaattc gcagaaaaaa acggactgcc aggttcccag
    23161 caccagatgt ccgttttcaa tgatgaccag ttcggaagct ccgacaaggg aagattttac
    23221 gtgcgctgcg gaattgcctt ccgcgtgacg gtaattcgcg gaaagcggca ctgccctgtc
    23281 cagcgctgca atcatgtccc gttgcacatc cggatcggcg ttttcattga tggtgattgc
    23341 ggccgtggta tgcggggtgt agaccagaca aacaccggac tgtacgcctt catcgcggac
    23401 ggcctcttga acagaggcgg tgatgtcgat catctccacc cgcgacaacg tcttcagaga
    23461 tatttctttc atgggacttt caccatcgtt tgccttttca attccattgt tttttagaca
    23521 gcaaatccgt tttttgtcaa atatgaatca aaaaagcggt ttttttataa atttatacat
    23581 ttcgttcaat agtggttaat gttttattta aagaacagga gcaaatgggc tcccttcccc
    23641 gttgatttgc cggccgccgc gtataatatt aatcaattaa ttcaataata taaacgcata
    23701 atacaataaa aaattatttt ttggcacggt cttttcatta gattaagaca aagaaaaaaa
    23761 tttcctaaag gaggcaacag atcatgaaaa aaacattagg aacaatgata gtggcggcag
    23821 cggttattct tttcacagca accttcagct ttgcggaata tgcggcagcc ggagcagcca
    23881 atttcccctt cttccagctg ggatgcctga tcgtcggcgg actgatcatg gtttctttga
    23941 aacgcaaata tgacaagatg tatgtcggtg aagtggtcgg catcttcgcc ctgtacacca
    24001 ttctgatggc gctgttcacc aacccggtca tcgaaacggt caaaaccatc gtcagctaaa
    24061 aaattaacag catctgaaaa acatgatctg ttaacgatag tccaatcccc tcaaggccct
    24121 cgggccttga gggttttttt cgtaaatact agatcaaaga aacgtcggta tagcggcacc
    24181 gtgtgacccg atagcaaggc ccgtgttgaa tggtgccata gccgatgata ataatttctt
    24241 caaaaaatcc cgatggacaa atcatgcgat cggtttataa tttatgtaaa ttatcaaaaa
    24301 atggagtgtt gatatgtcgg acgggttgag gaaaatcgca tttatagtca tgctgctggc
    24361 ggcactaggc tggattgcgg ggtgcgccgc aggttctcag gcgttgctgg ccgaagatta
    24421 caaagccatg gataacgaaa atcttctccg ttattactac aggctcaatg atgaaatcga
    24481 gcgtcaggag aaaagatccg gtccgcaatt cagtgtcggc atcggcggtt tcggcggatc
    24541 catcggcggc ggtgcggaag tcggcgccgg aggaacgcgc tacacggctc atgatttgag
    24601 ggcgcgaaga attgatgcgc tgatggaatt gaagaaaaga ggagtgaccc cctaatttac
    24661 agagagcatt tttgaagaaa aataaaagag ggctcctgat ggcaggggcc ctctttttat
    24721 ctggcgcgcc caggaggatt cgaacctccg acatccagat tcgtagtctg gcgctctatc
    24781 cagctgagcc atgggcgctt ttataatttt taaaagacgg tcgtcaagag gccgttcatc
    24841 aacgacggcc ctgtcacaac cccatttcgc ttacgcgtca ggggtaattc tcatcaatct
    24901 tcagttcgct tcgtttctga agattgggcc tcatttccct ccacgatgtg ggattgttca
    24961 acctacaggt ttcagagcac tttgtttctg aaacctgggt ctcattaact taccaattct
    25021 gctgcgctgc gctttcagca ttggagtttc acaaaaaatg gcggagagag cgggatttga
    25081 acccgcggta cacc
//
LOCUS       DAOSME010000031        24443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2586_length_24443_cov_21.270912, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000031 DAOSME010000000
VERSION     DAOSME010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 24443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24443
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2586_length_24443_cov_21.270912"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="rmuC"
                     /locus_tag="PLG98_06255"
     CDS             <1..522
                     /gene="rmuC"
                     /locus_tag="PLG98_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOE32893.1"
                     /translation="GDVAAADESVRQLETGIKKAARDIFQKYIAPPKTTDFGIMFLPS
                     EGLYAEVIRRTALVGVLQREYHIVVTGPTTFAALLNSLQMGFRTLAIQKRSGEVWKVL
                     GEVKTAFGRFGDTLDNVRKKLEQAANSVDDAQKKTKTLSNKLKAVEASTDTRPTDDGI
                     TDDMPSHRASEHE"
     gene            555..1955
                     /locus_tag="PLG98_06260"
     CDS             555..1955
                     /locus_tag="PLG98_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32894.1"
                     /translation="MNDITMERRHEDRRHDDRRRGERRKYERREDVRKMNQELIREQM
                     EKYRVLLKDIKDAYFEVDLLGNFTVVNSAMCSALGYGPEELIGSKYRKLVDKDGAKKL
                     QQAVERLHKNDEPVNLLDMEAAKKDGTKIIYEAMVSVIRETDGVVVGFRGVGRDVTRR
                     RQMEAELKRNQHDLIRKNQEIDQSRKDIQQALVSLEKAHEELKASQLKILQQEKMASI
                     GQLAAGVAHELNNPMSFISSNLSTLEKYINRLIQFIRVQSEAVASLQDAAVVRRVDEK
                     RAEVKLDYMVEDVRGLLKESLEGAQRVKKIVYELNSFSRMDEEEYKKADIHECIESAI
                     TIVWNELKYKSKLEKHYGDLPATMCYPRQMNQVFVNLLINAVNAIVDKGTITIKTWYE
                     DHAIRIDVSDTGAGIPRENLIRIFEPFFTTKAVGKGTGLGLSITYEIIQRHRGEITVA
                     SEEGRGTTFSIKIPVV"
     gene            2196..3338
                     /locus_tag="PLG98_06265"
     CDS             2196..3338
                     /locus_tag="PLG98_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE32895.1"
                     /translation="MSKNIFRIIFVLIFSFLTAGVVLAAPPVKIGALFAVTGPASPLG
                     EPERNTAVMMVDEINKAGGIKGRKLELIVYDTQGDATKAVQAVNKLIKDDKVVAIIGP
                     STTGDSMAVIPVIEKSQVPLISCAAGIKITDPVKKWVFKTAQNDVLAVMRIYKNLQKQ
                     KISKVAILTVSDGFGSSGREQLRLQAKEFDISIISDETYGPKDTDMTVQLTKIRNSAA
                     QAVICWGTNPGPAIVARNARQLGIKIPLYMSHGVSSKKFIELAGEAAEGIMLPSGKVI
                     VADQLPDSDPQKKILLNYVSKYKERYKVEGDHFGGHAYDAVMLLKRAMEQGGFTPAAI
                     RDQLEKTKKFAGIGGTFSFSSRDHAGLTSDAFVLVRVEKGDWKLIN"
     gene            3548..4432
                     /locus_tag="PLG98_06270"
     CDS             3548..4432
                     /locus_tag="PLG98_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE32896.1"
                     /translation="MDNFQQLFQYVLTGLSNGAIYALIGFGFAVIYNSTGIINFAQGE
                     FVMLGGMLTVFLLTVLHVSLVPAIALAIVVSTLVGILFERFAIRPLKDAQPLSLIIIT
                     IGASIFIRGVAMLVWGKDTHALPAFSSHDPLSIAGATILPQHLWILGVTILIIAASRL
                     FFNHSIIGKAMRACSYNPRAAGLVGINVKSMVLLSFSLSAAIGSLAGIIIAPLTMTSF
                     DVGVMLGLKGFCAVIIGGMSSGMGTVLGGLLLGLLEALGAGFISASYKDAIAFIILLL
                     ILFIRPEGLFRKKETERF"
     gene            4482..5453
                     /locus_tag="PLG98_06275"
     CDS             4482..5453
                     /locus_tag="PLG98_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE32897.1"
                     /translation="MLKEKKHQIFIFVIFALAVLSVPFFLQGNYLLNVLVFVGINTML
                     AVGLNLLLGYAGQISLGHAAFFGMGAYISGIVTSRYPVEPFLIIFLAAVCVGLIAFVI
                     GFPILKLKGHYLAMATLGFGIIMYIIFNETVDWTGGPSGLSGIPSLKIGPVVFDNDQS
                     NYYLVWVFTLVVMMLSINLSNSRIGRALRAIHDSEVAARVMGVNARFLKVQIFTVSAV
                     ISAVAGSLYAHVMTFIAPASFGFNFSVELLTMIVIGGLGSVYGSFLGAVILTMMPEFL
                     RVFQDLDIVIYGLMLILMTMYMPGGLISGIEMAAEFFSSRLRKGKGE"
     gene            5450..6205
                     /locus_tag="PLG98_06280"
     CDS             5450..6205
                     /locus_tag="PLG98_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32898.1"
                     /translation="MMLRLSNITQIFGGLNALEDVSFSVREGSITGIIGPNGAGKTTL
                     FNIVTGLYTQTGGDVFLGGENISFLETEELAGRGLVRTFQNVELFRKMTVLENVMVGL
                     HTKSSSGIFSCVFKLPRQTREEKAIREKSMVWLKYTGIAELAEMTAGNLPFGKGRLLE
                     ISRALAAGPKIILMDEPAAGLNSRETAELALLIRKIRQSGVTVVLVEHDMDLVMDVCD
                     AIVVLNLGKKLAEGTPREIQENPAVISAYLGEG"
     gene            6319..7062
                     /locus_tag="PLG98_06285"
     CDS             6319..7062
                     /locus_tag="PLG98_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE32899.1"
                     /translation="MLKIKNINTYYGQAQALKNVSLHLAEGEIVTLIGANGAGKTTLL
                     NSLSGVVPTRSGQIIFNDAPINNLPVHQIVRLGISQVPEGRQVFKPVSVEDNLELGSY
                     LHHRILGGARDVKKNKEMVYDLFPILRERRKQLAGTLSGGEQQMLAIGRAFMTRPKLM
                     LLDEPSMGLAPMVVQEIFRVIASLSREQKTTVLLVEQNARAALKMAGRGYVLETGHMI
                     LEGTAAELLNNKDVQRAYLGRDKKEIWER"
     gene            7142..8443
                     /locus_tag="PLG98_06290"
     CDS             7142..8443
                     /locus_tag="PLG98_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE32900.1"
                     /translation="MNIWDQTHECMARDELEQLQLERLQAVVNRVYKNVTHYRKIFNE
                     TGIIAEDIRSLADLSRLPFTTRDDLQVNYPYGMFAVPLREVVRIHSSSGTTSKPIVMG
                     YTKNDIKIWSNLAARFMTAAGVTPDDVVQITFRYGLFTGAFGVHYGAEAIGASVIPMG
                     TGNTDKQIMIMQDYKSTVLVSTSSYAIALADRLDQLGIDPKSLSLKTGLFGGEPWSEK
                     MREEIENRLLLKATDNYVISELIGPGVAGECLCKKGMHIYEDAFIPEIIDPETGAVLP
                     AGAEGELVLTTLTREAFPLIRYRTGDVTSLDYTPCECGRTLVRMKKIMRRSDDMIIVR
                     GVNVFPAQIEDALFSVAQGETPYQIVVEREGAMDNLEVVVEVTDKIFSLELQKQRSFL
                     ETIKKRLASVTGINVVVRLVESKSIPRQQGRIQRVLDKRQI"
     gene            complement(8456..9520)
                     /gene="bioB"
                     /locus_tag="PLG98_06295"
     CDS             complement(8456..9520)
                     /gene="bioB"
                     /locus_tag="PLG98_06295"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOE32901.1"
                     /translation="MNFVDHLKNKIIRQSRFITPAEAMELFIEASGFCWSVLAAASEI
                     REHFKGKKIIFCGITNAKSGRCPEDCMFCAQSSHYKTTAHSYPLKKSAQIIADAEKAV
                     RNGAECFGIVTSGKQLKSKKERLEIIRAIQGVRETGLFPCASLGMIDKATAMELKAAG
                     LFRYHHNLETSRSFFKTVCTTHDYEESVKTLCAAKEAGLSVCSGALIGMGENISHRIE
                     LAQTLRKLDVDSVPLNILHPVKGTPLSHMRALPPMEILMTLAVFRFMLPDRDIRLCGG
                     KEKNLRQLLPLGIVAGANALMTGDYLTTAGRNSSLDHEMIADLGLTATRERITCTCNI
                     EKHSRPVARQKRRPVQKHPS"
     gene            complement(9517..10221)
                     /locus_tag="PLG98_06300"
     CDS             complement(9517..10221)
                     /locus_tag="PLG98_06300"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOE32902.1"
                     /translation="MDKRLAYRIFFLFLFIALSAIIFIHYDLHHLFRDRDRLVNFILS
                     YPYDQFIFILVQIIQVVAAPIPGELTGIIGGYLYGPLWGTLYSTIGLTIGSWIAFMLA
                     RFFGEPLLENVVKKEVFDKFDHFMEHKGLLVSFLLFLIPGFPKDYLCYIMGVSRIPVG
                     TFIIISTAGRIFGTIMLSITGNVARNGQYILLTVILILGVTVFIAVYYYHDQLINILK
                     KEGAKLGHVKKERKSK"
     gene            10464..11822
                     /locus_tag="PLG98_06305"
     CDS             10464..11822
                     /locus_tag="PLG98_06305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOE32903.1"
                     /translation="MAKIPQKLERKKSDIYKDAPIAKFGERKPDFSTMGRKMKNPHAR
                     FREVVCVEACRTPYGRSGGALKNFSAMELGAMAIKEVLRRTGGKVAPSDVDYIFMGQV
                     VPAGCGQIPGRQATILAGVPEFVPSITVNKVCSSGIKTVDLAFQMILLGRAEICIAGG
                     QESMSNCPFVLPDMRWGAKMALPNGRVVDSMVYDGLWDAFYNRHMAIHGSEVADEFGF
                     SRQEQDEWALNSQMNAVRAMQEGRLDDEVFPVEVRQGKAILIMDKDEGPRPETTLEGL
                     AKLPPVFNHTSTVTGRPGSVTAGNAPGVNDGGDVCLLMSRAKADELGLKPIFTLVDYA
                     EVSQPTKDIATVPGLAIKKILDQNDMTLDQMEVIEINEAFAAVALVSCRSILGMSRET
                     MFKKVNVNGGAIAYGHPIGATGARILMTLGYELRRRGGGWGVCGICSGHAQGDAMLIR
                     VD"
     gene            12044..12814
                     /locus_tag="PLG98_06310"
     CDS             12044..12814
                     /locus_tag="PLG98_06310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE32904.1"
                     /translation="MAGNPDMSIVVPVYGSDITLMPLYCRTVAAVSPISENFEFILID
                     DCGPGKPWEIIGAMAKKDSRVIGLKLAKNVGQHNAIKAGVDVAHGQWVVVMDCDLQDR
                     PEEIPRFWGKAQEGYNVVIGRRVERRDETIKRIFSYIFHGLLKWVTRQNSDAAQSNFG
                     IYSRQVIDKIKKLPEQSFYLPLLVRRAGFDVIPLDVEHDKRPYGKSGYTFSRRLSLAV
                     DILASSGDHPLKMFSLFRFFNASRGKPLYVVTDQIRMT"
     gene            12936..14087
                     /gene="rffA"
                     /locus_tag="PLG98_06315"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             12936..14087
                     /gene="rffA"
                     /locus_tag="PLG98_06315"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOE32905.1"
                     /translation="MKNKIEIPFNKPYIADKELIYIARAVMENNHTAGDGPFTKKCQI
                     WLEEKLHCRKALLTHSCTAALEISAMLADIQPGDEIIMPSYTFASTANAFVLRGGIPV
                     FIDIRPDTLNLDEALIEAAVTPRTRVILPVHYAGVPCEMDVIADIARRHQLFVIEDAA
                     HGILCSYKGRPLGSIGHFGACSFHETKSMISGEGGALLINDEKYVERAEIIREKGTNR
                     SQFVRKGLSQYTWVDVGSSYLPSDIIAAFLYAQMENVDQMISRRNAIYDVYWKQLEPL
                     EEKGLIRLPTQRDDCFRNGHIFYVITGSLRERIDLASFLKDRGISAFFHYVPLHSSPA
                     GKVYARTCGALEVTTAISNCLLRLPLYYAMTEEDVQAVVRAIMDFYRVR"
     gene            14153..16054
                     /locus_tag="PLG98_06320"
     CDS             14153..16054
                     /locus_tag="PLG98_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32906.1"
                     /translation="MDEREENGSNPVAAGNDAPQHPLRASSFFAFSEKLSASVNLRWL
                     MISLLPVLILLKYPVDRIDYDVWWQMAHGQYYLTHHTLKMDLSMFSWTPTDPTWIYNT
                     CLGSIAVYLFYHWAEGFGLWLFQWLIFSGVFLSFYLFLRSIHQRLDINSATVIAAVAM
                     ACSISCRYYKPELLSALLFCWTAHIFFHIKIRGNRYLFYLFPPIFAFWVNLHGAFIVG
                     LAFLGLAFAGEFLNRIFFPKKSLKTEDLMHFGLALILSGAATLLNPYGIDYLKSLVPT
                     IMNAVGVGDYSGPYDKFVLAYASLWPYLRKMGPELFGVGMTAWILTLMMMSIAFLVAY
                     DLMKHKSCDFALCIVSLALYWKGMETSRATYFFPIVFFFIFYYLLVSRLQMTKFGSKT
                     TVFSIMVFIFFFGMVSFFNIKYGADDKWFGRDLDGYVPVEEVEFLKKYKLPGPIFNDY
                     VIGGYLLWKLYPDYKVFIDPRGGLYRNQVFPDYMEFTIRHVSRDDIRRFREKYPFNIA
                     ILHYRQMALIFDFLKAGDDWRLLYFEKNAAILIHQSLLPLIEPIAGNISLSPIRFKHV
                     RNPEILTNVFNFYVRLDPRAGRYIYYVFKRNVSDGFFMKKEILTAMDSNIRLKEKAFQ
                     ERPDLFFHE"
     gene            16161..16901
                     /locus_tag="PLG98_06325"
     CDS             16161..16901
                     /locus_tag="PLG98_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE32907.1"
                     /translation="MSSDAFKMFISEIDQERFGIKTARVVDMTADRLPSVLDFCVSHA
                     VKLLIARCSISDLGAAQSMEKQGFLLMDTLVYYTFDLLRRPVSSSDDDVHFRPIRRGE
                     ENVVERVAIESFRGYFGHYHADPRLDRDKCDDVYVDWARKACVAKGSDENFMVAEIQG
                     RIVAFGVFRMNNPDEGELFLGGIHPDFRGQGIYQSFLHRAKAWCLSKDARRMVISTQL
                     NNIAVQKILVKFGFEITRGCYTFHKWFD"
     gene            17110..>17456
                     /locus_tag="PLG98_06330"
     CDS             17110..>17456
                     /locus_tag="PLG98_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32908.1"
                     /translation="MTAILVPLLIFLAYPLPRGDYDLWWQMALGKYYLTHHTLVIDHS
                     IFSWTPADSGWIYNTFLGSIILYLMYHCMGGFGLWLFQWLIFLGIFLSFYLFLRSTGQ
                     RLDVTALTVIAAIG"
     assembly_gap    17457..17556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17557..18168
                     /locus_tag="PLG98_06335"
     CDS             <17557..18168
                     /locus_tag="PLG98_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32909.1"
                     /translation="GLDESVPVAEVAFLKKYKLEGPIFNDYLLGGYLIWALYPDYKVF
                     IDPRLVPYHKQVAPDYWQLERMLPTPEDMRRFNQRYPFKTAIIHYNRHFLIEDFLKAG
                     WKLVYFEKNAAVLVHESMLAAVPPEVQWVDLGPSRFRNVKDPVVLLNVFNIYVNVYPE
                     ASRVIYDIYRSNVSTWYEPREQHLRAMEEDMRKMQSLQRKAKS"
     gene            complement(18209..19951)
                     /locus_tag="PLG98_06340"
     CDS             complement(18209..19951)
                     /locus_tag="PLG98_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOE32910.1"
                     /translation="MFITLAAVLFFGLHQAEGAFNESMAIDTKAISLANNVTADPPGT
                     ASIHYNPAGLSFMGDGSYISAGLIPAVLKMSGTFESDPNHQAFHDYQGNVIVDPLAGR
                     SSSTSTGRMFIPILNDTAPAVMGPIFGLSYRKPGSKWTFGYTTYAQYGGGWEYGDDDP
                     AMYGAKSVYLQHLIYAAPAISYRINNNLAVGASFGIGQTAMGLELKMRGPNEIVNITK
                     ILGDATQGMANPLFDVTVPLPLFAGGVGVYDNIGDLSLSLRDDFSPSYNLGVLWEPFD
                     WVALGLCYQSAVKSNVSGKFSLKYSEDWQRMVAWSGSTAVMQISSIIFDLPYTATDEQ
                     KGTVSTTLEFPQIVNVGVKLKPTKRLSLLGEVHWAEWSAVKENDLKFDQKVQFLQMAK
                     FMGYSGGSYNMKVAREFKDTLNWGVGVEYQLLDWLQLRAGYENRTSSTIDEYYDLMYA
                     LPSMRYFGAGMGIQWKDIDIDLALGYMISKSFKIRPGQSKNMNSYTLGDGLNTSYRGL
                     SYEQEVSVYLASLKATMPLHIVTDMIFGLLPFKFGSSKGSKVADLKEPVDSSANFMNN
                     MRYKNKYYFIEDSE"
     gene            complement(20163..21398)
                     /locus_tag="PLG98_06345"
     CDS             complement(20163..21398)
                     /locus_tag="PLG98_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOE32911.1"
                     /translation="MKRKFVIVLSLLFFLTFTLLAALPVMANEPLEGIQLALSGVSAD
                     DANAAADQEAVKAPKETTDVQETKAVEAPPVAEPQKPVTTQEGSAKSIEQELKELKEE
                     IQKLRGENEARKKLEIPEEEKSQTIDDILSAAGRQYSLLKKGTVGLSYTFNYAYYSGD
                     AIDESTTVLRRVNHNLTNTLALEYAFFNNLTVSSNFPFVYKYNKVGTDESLDVTDLGD
                     ISIGFSWQPFKAGGRIPTTILSLSLSLPTGSSPFEINYEDELATGSGYYGIGAGVSFS
                     KVLDPLVAFGNLNYTYGIPESGLSQILYEDPSDPASKIYLEKVEPGSSIGVSMGFGYA
                     LSYQASLNMSAQFSYSFGSKYKSNTGTTETGSSLSSSFNVGTGWRITPSRSIYATVSM
                     GLTNNDPDVSLSLRLPYEF"
     gene            complement(21876..22991)
                     /locus_tag="PLG98_06350"
     CDS             complement(21876..22991)
                     /locus_tag="PLG98_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE32912.1"
                     /translation="MILFLLIFLSLYGGINFYAFLKAKTLFHFSGKIEIIIIISMIFL
                     ILAPIIIKLAEKIHWETLARSIAYCGYLWMAFVFLFFVIHVSFDLIRPLLKIIFQTPG
                     GDSLKNILFFTALFLSLSFVVYGFFDAKNIQVKKLEIHTEKLPPGHETLRIVQISDVH
                     IGLIVRGKRLQNILEKVREAKPDILVSTGDLLDGELDNIASEAKRMMEIKPKFGKYAI
                     MGNHEYYAGIEKSLAFTKSAGFEILRDESKKIAGIHMIGLDDPTGRGYGVSEKNPSLS
                     NLFPVKKSKEFILLLKHQPTIREDKNFDLQLSGHTHGGQIFPFMFLTRIFFPKNSGYH
                     QLSDNKSVYISKGAGTWGPPVRFLAPPEITIIDVIKK"
     gene            23091..23166
                     /locus_tag="PLG98_06355"
     tRNA            23091..23166
                     /locus_tag="PLG98_06355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23124..23126,aa:Thr,seq:cgt)
     gene            complement(23358..>24443)
                     /locus_tag="PLG98_06360"
     CDS             complement(23358..>24443)
                     /locus_tag="PLG98_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE32913.1"
                     /translation="TFPEQTLQYFEEADFAIRGEAEKGLTDLITHLSQPDQIAKDQIP
                     GLIWRESGAIRCNPPLFIENLDALGSPDWDLINPCHYPFQTSYFARSKIVAPLIMTRG
                     CPYHCTFCSCRAITGHKIRAHSVSYMIHQIKLLQSRFGIREVCFIDDNFLARKDLVME
                     LCEKILAENLDIRWSCFGIRLDLVDEQVLDLMEKSGCYLLTVGIESGSQRILDHMKKK
                     LTIELIEEKIKLINSKTRINIIGNFIIGYPAENEDDIRKTIKFAGKLPLYGANFFPFH
                     PTPGTEIFNQLQTQKDFEFHHWDLMGQDMKPYVPEGMSRKKFTWLFALAFINFYLRPK
                     VIRNVLRATKSLDKLKYIAYRIFKIIR"
BASE COUNT         6214 a         5424 c         6215 g         6490 t
ORIGIN      
        1 ggggatgttg ccgcggcgga cgaatctgtc cgccagctcg aaacaggcat taaaaaagcc
       61 gccagagata tttttcaaaa atacattgct ccgccgaaaa ccacggattt cggcattatg
      121 tttcttccgt cggaaggact ttacgccgaa gtcatacgcc ggacggctct ggtgggcgtt
      181 ttgcagcgcg aatatcatat cgtggtgacc ggtcccacaa cgtttgccgc gcttttgaac
      241 agtctgcaga tgggtttccg cacgctcgcc attcagaaac ggtccggcga agtctggaag
      301 gttctgggag aggtcaaaac ggctttcgga agattcggcg atacgctgga taatgtgcgc
      361 aagaaactgg agcaggccgc caattcggtc gatgatgcgc agaagaaaac aaaaacactt
      421 tccaataaac tgaaagccgt ggaagcgtcg acggacacac ggccgacgga cgacgggata
      481 acggatgata tgccgtcgca ccgggcaagc gaacatgaat agtcaacgca atacctctgg
      541 aggggatcga aaccatgaat gatataacga tggaacgccg tcatgaagat cgccggcatg
      601 acgaccgtcg acgcggtgag cgccggaaat atgagcgccg cgaagacgtg cgcaaaatga
      661 atcaggaact tattcgcgag caaatggaaa aataccgcgt gctgctcaag gatatcaagg
      721 atgcgtattt tgaggtcgat cttctcggta atttcaccgt cgtcaacagc gcgatgtgca
      781 gtgctctggg atacggaccg gaggaattga tcggctcaaa ataccgtaaa ctggtggaca
      841 aggacggtgc aaaaaaactt cagcaggccg tggaacggct tcacaaaaac gacgaaccgg
      901 tcaatctgct ggacatggag gcggccaaaa aagacggaac gaaaattatt tacgaggcga
      961 tggtttccgt gatcagagaa acggacggcg tggtcgtggg attcagaggg gtcggccggg
     1021 atgtgacccg ccgccggcag atggaagctg aattgaaaag aaatcagcac gatctgatca
     1081 gaaagaatca ggaaatcgat cagagccgga aagacattca acaggcgttg gtcagccttg
     1141 aaaaggctca tgaggaattg aaggcgtcgc agttaaaaat ccttcagcag gagaagatgg
     1201 catcgatcgg gcagcttgcc gccggcgtag cgcatgaact gaacaatccc atgtccttta
     1261 tttccagcaa tctcagtacg ctggagaaat acatcaaccg tctgattcag tttatccgcg
     1321 tacagtccga ggccgtcgca tcgcttcagg atgccgccgt tgtgcgccgt gtcgatgaga
     1381 aacgcgcaga ggttaaactc gattacatgg tcgaagatgt ccggggactg ctgaaagaat
     1441 ctctggaggg ggcgcaacgg gtcaaaaaaa ttgtctatga actcaactcc ttttcccgca
     1501 tggatgaaga ggagtataag aaggcggata ttcatgagtg catcgagagc gccatcacca
     1561 ttgtctggaa tgaattgaaa tacaaatcaa aactcgaaaa gcattacggc gacctgccgg
     1621 ccaccatgtg ttatccccgg cagatgaatc aggtgttcgt aaatctcctg atcaatgccg
     1681 tcaacgcgat tgtcgataaa ggaaccatta cgattaagac gtggtacgaa gaccatgcta
     1741 tccgtattga tgtttcggat acgggtgccg gcataccgcg tgaaaatctg atccggatct
     1801 tcgaaccttt ttttaccacc aaagcagtgg ggaaggggac cggtctggga ctgagtatta
     1861 cctatgaaat tattcagcgg cataggggag agataacggt tgccagtgag gaaggcaggg
     1921 gcacaacgtt ttcgataaaa attcctgttg tgtgaagacc ggaagatttt ttaaaaatat
     1981 tccggtctgc cggcggcggg gatgaatttc cgtttgacac agaaagtgtt ctgtggttta
     2041 caactcgtca aaaatattaa gctattggaa agacagaccg ggcgaatttt taatcaaagg
     2101 ggttagtcgt ttcaccggcg tattgccggc attggagctt ttaagttttt ttattcgttt
     2161 gaatggttgt ggaataaact taagtggagg tgtgtatgtc gaagaatatt ttcagaatta
     2221 tttttgtatt gattttcagc tttttaacgg cgggggtggt gttggcggct cctcccgtaa
     2281 aaatcggcgc gctgtttgcc gtaaccggtc cggcatcgcc gctgggcgag ccggaacgca
     2341 atacggcggt gatgatggtt gatgaaatca acaaagccgg cggaatcaag ggccgcaagc
     2401 tggaactgat tgtctatgat acgcagggag atgccaccaa agccgtccag gcggttaata
     2461 aactgatcaa ggatgacaaa gtcgtggcaa tcatcggtcc gagcacgaca ggcgacagca
     2521 tggccgttat tccggttatt gaaaaatctc aggttccgct gatctcctgc gcggcgggca
     2581 ttaaaatcac cgatccggta aaaaaatggg ttttcaaaac agcgcagaac gatgttctgg
     2641 cggtcatgcg gatttataaa aatttgcaaa agcagaaaat ttccaaagtg gctattctca
     2701 cggtctccga cggattcggg tcttccggac gcgaacagct gagattgcag gcgaaagaat
     2761 tcgatatcag cattatttcc gatgaaacgt acggtccgaa agacacggat atgaccgtgc
     2821 aattgacaaa aatacgcaac agcgcggcgc aggccgtcat ctgctggggg acgaaccccg
     2881 ggccggccat tgttgcccga aacgccagac agctggggat caagattccc ctgtacatga
     2941 gtcacggtgt ttcttccaag aaattcattg aactggccgg agaagcagcg gaaggcatta
     3001 tgctgccttc aggcaaggtg attgttgctg atcagttgcc ggattcggat ccgcagaaaa
     3061 aaatcctgtt gaactacgtg agcaaataca aggagcgcta taaggtggaa ggcgatcatt
     3121 tcggcggcca cgcatatgac gcagtcatgc tgctgaagag agccatggaa cagggcggat
     3181 tcactcccgc ggccattcgc gaccagttgg agaaaaccaa aaaatttgcc ggtatcggcg
     3241 gaacgttcag tttctcttcc cgggatcatg cgggtctgac cagcgatgcc tttgttctgg
     3301 tcagggtcga aaagggcgac tggaaattaa tcaattaacc gatccggcat tcggattcat
     3361 ttttttataa acggcgcgga accaaaaccg cgccgttttc tgttcgtcca gtgactcaaa
     3421 cataaattga taccatttct aaattaaaaa tgatattgaa tccacgattg cctgcgtttt
     3481 acagacggcg gaatcggcgg ccacccggcc tgtgtgacgc gtattcgatg cagagacgga
     3541 atgggatatg gacaattttc aacagctatt tcaatatgtt ctgaccggtt tatccaacgg
     3601 tgccatttat gccctgatcg ggttcggatt tgccgtgatt tataattcca ccggaatcat
     3661 caatttcgcc caaggggaat tcgtgatgct cggcggcatg ctgactgttt tcctgctgac
     3721 cgttttgcat gtgtcgctcg tgccggccat cgcactggcc attgtcgtgt cgacccttgt
     3781 cggaatttta ttcgaacgtt tcgccatccg gcctttaaaa gacgctcaac cgctgagtct
     3841 gatcattatc acgatcggcg ccagtatttt tatccgcgga gtggcgatgc tggtctgggg
     3901 aaaagataca cacgccctgc ccgctttttc cagtcacgat cctctctcaa tcgccggcgc
     3961 cacgattctg cctcaacatc tctggatcct tggcgtgacg attttgatca tcgcggccag
     4021 caggctattt tttaatcaca gcatcatcgg caaagccatg cgcgcttgtt cctataatcc
     4081 gcgcgcggcc gggcttgtcg gcattaatgt gaagagcatg gtgctgcttt ctttttccct
     4141 gagcgccgcc atcggttctc tggccggtat tattatcgcg cccctgacga tgacgtcctt
     4201 cgatgtcggg gtcatgctcg gcctgaaggg attttgcgcg gtcattatcg gcggcatgag
     4261 cagcggcatg ggaacggtgc tgggcggtct tttgctgggg ctgctggagg ctctgggcgc
     4321 cggttttatt tccgccagtt acaaagatgc catcgcgttt atcattctgc tgttgattct
     4381 atttattcgt ccggagggat tgttcagaaa aaaggaaacg gagcggtttt aggcgccggc
     4441 cgcggataaa ccgtttacgg attggtgcat tatatttgat catgctaaaa gaaaaaaaac
     4501 atcagatatt catcttcgtc attttcgcac tggccgtgtt gtcggtcccg tttttcctgc
     4561 aggggaatta tcttttaaac gtgctcgtgt ttgtcggcat caacaccatg ctggcggtgg
     4621 gactgaatct gctcttggga tatgccgggc agatttctct ggggcacgcc gcctttttcg
     4681 gcatgggcgc ttacatctcc ggcatcgtga cgtcccggta ccctgtcgag ccgtttctga
     4741 tcatttttct ggcggcggtc tgtgtcggcc tgattgcttt tgtgatcgga tttcccattt
     4801 tgaaactcaa ggggcattat ctggccatgg ccaccctggg cttcggcatt attatgtaca
     4861 ttattttcaa tgaaaccgtg gactggacag gaggaccttc gggcttgtcc ggcattccca
     4921 gtttgaaaat cggtccggtt gttttcgata atgatcagag caattattac ctggtgtggg
     4981 tttttacgct ggttgtgatg atgctctcga ttaatttgtc caactcgagg atcggccgcg
     5041 cacttcgcgc gatccatgat tcggaagtgg ccgcacgcgt gatgggcgtc aatgccaggt
     5101 tcctgaaggt gcagattttt acggtttctg ccgtgatttc ggccgttgcc ggcagtcttt
     5161 acgctcacgt catgacgttt atagcgcccg cgtcgttcgg ttttaatttt tccgtggagc
     5221 tgctgacgat gattgttatc ggtggtctgg gcagcgttta cgggtcgttt ctgggggcgg
     5281 tcatcttgac catgatgccg gaattcctgc gcgttttcca ggatctggac atcgttattt
     5341 acggattgat gctgattctg atgacgatgt atatgcccgg cggtctgatc agcggaattg
     5401 agatggcggc agaatttttt tcgtcccgcc tgagaaaagg caaaggtgaa tgatgcttcg
     5461 actcagcaac atcacgcaga ttttcggcgg actgaacgcc ttggaggatg tgtcgttttc
     5521 ggtcagggaa ggatccatta ccggcattat cggtcccaac ggggccggga aaaccacgct
     5581 tttcaatatc gtgaccggcc tttacaccca gaccggaggg gatgttttcc tcggcggaga
     5641 aaatatttct tttctggaaa ccgaagaact tgccggccgg gggctggtgc gcacatttca
     5701 aaatgtggaa ctgttccgga aaatgactgt tctggaaaac gtgatggtcg gtttacacac
     5761 gaagagttca agcggcattt tctcgtgtgt ttttaaactg ccgcgtcaga ccagggaaga
     5821 aaaagctatc cgggaaaaaa gcatggtatg gcttaaatat accggcattg ccgagctggc
     5881 ggaaatgacg gccggaaatc tgcctttcgg caagggacgg ctgctggaaa tctcccgggc
     5941 gctggctgcc gggccgaaga tcattctgat ggatgaaccg gcggcaggat tgaacagcag
     6001 ggagaccgcc gaactggcgt tgcttattcg caaaataaga caatccggcg taacggtcgt
     6061 tctggtagag cacgatatgg acctggtgat ggatgtctgt gatgctattg tggtcttgaa
     6121 cctgggtaaa aaactggctg aggggacacc gcgggaaatt caggaaaacc ccgcagtcat
     6181 ctccgcgtat ctcggtgaag gataatgagg tttttaaaaa attgtccgat gctgcagcgt
     6241 gcaacaaaaa tgcgtatcaa cccttgtctc gaccgcgtct aatcgccggt atttttaggg
     6301 agccgttggg taaacaccat gttaaagata aagaatatca atacatatta cggacaggcg
     6361 caggcgttga agaacgtttc gctgcatctt gccgagggcg aaattgtcac actgatcggc
     6421 gccaacgggg ccggcaaaac caccctcctg aattctttat caggggttgt gcccacgaga
     6481 agcggtcaga ttatcttcaa cgacgcgccg atcaataatc ttccggttca tcagattgtg
     6541 cggcttggta tttctcaggt tccggaaggc cgtcaggtgt ttaagcctgt ctccgtggaa
     6601 gataatctgg agctggggtc ctatctgcat cacaggatac tcggaggcgc ccgtgatgtt
     6661 aaaaaaaata aagagatggt ttatgatctg ttccccatcc tccgggagag acgaaaacag
     6721 ctggccggaa ccttgtccgg cggagagcag cagatgctgg ctatcggccg cgctttcatg
     6781 acccggccga agctgatgct gctggatgaa ccttccatgg ggctggcgcc gatggtggtg
     6841 caggagattt tccgggtgat cgcctcgttg tcgagagaac agaagaccac cgttcttctg
     6901 gtggaacaaa atgcgcgcgc ggcgctgaaa atggccggcc gcggttatgt tttggaaaca
     6961 ggacacatga tcctggaagg gacagctgct gaactgctca acaacaaaga tgttcagcga
     7021 gcgtatctcg gcagggataa aaaagaaata tgggaaaggt aagcagccga agtttggaga
     7081 agaacggtcc gtcaacgtca tcaatatttt tcaacaactt tccttttacg gaggttattt
     7141 tatgaatata tgggatcaga ctcacgagtg tatggcgcgc gatgagctcg agcagctgca
     7201 gctggaaaga cttcaggccg tggtcaaccg ggtttacaaa aatgtcacgc actatagaaa
     7261 aatatttaac gagacaggca ttatcgcgga agatatccgt tccctggccg atttgagccg
     7321 gctgccgttt accaccagag acgatttaca ggtcaattat ccctatggga tgtttgccgt
     7381 tccgctcaga gaggtggtgc gcattcattc ttcctccggc accaccagca agccgatcgt
     7441 gatggggtac acgaaaaacg atatcaagat atggtcgaat ctggcggcgc gttttatgac
     7501 ggctgccggt gtgactcccg atgatgtggt gcagattact ttccggtacg gacttttcac
     7561 cggcgcgttc ggcgtgcatt acggggccga ggcgatcggt gcctcggtca tcccgatggg
     7621 aaccggaaat acggacaagc agattatgat catgcaggat tataaatcaa cggttctggt
     7681 cagcacatcc agttatgcca ttgccctggc ggatcgtctt gatcaactgg gtatcgatcc
     7741 caaatccctt tccctgaaga cgggactctt cggtggcgag ccctggtcgg agaagatgcg
     7801 cgaagaaatt gaaaacaggc ttttgttgaa agcgaccgac aactacgtta tttccgagct
     7861 gatcggtccc ggcgttgccg gtgaatgttt gtgcaaaaaa ggcatgcata tttatgaaga
     7921 tgcctttatt ccggagatta ttgatcctga aacgggggcg gtattgcctg cgggcgccga
     7981 gggcgaactg gtgctcacga cactgacccg tgaggctttt ccattgatcc gctaccgcac
     8041 gggagatgtg acctcgttgg attatacgcc gtgcgaatgc ggccggacgc tggtccgcat
     8101 gaaaaaaatc atgcgccgtt cagacgatat gatcattgtc cgcggcgtca atgtctttcc
     8161 ggcgcagatc gaagatgccc tgttcagcgt ggctcagggg gaaacaccct atcagattgt
     8221 tgtggaaagg gaaggggcca tggacaatct ggaagttgtg gtggaggtga cggataaaat
     8281 attttctctg gagttgcaga aacaaagatc gtttctggaa acgattaaaa aacgtctggc
     8341 ttcggtcacg gggattaatg tcgttgtccg cctggtggaa tcaaaaagca ttccgagaca
     8401 gcaaggccgg atccaacggg tgctggataa acgacagata taaacgacac cgggattatg
     8461 aagggtgctt ttgaaccggt cgtctttttt gacgggccac cggccggctg tgcttttcga
     8521 tgttgcatgt gcaggtaatc cgttcccgcg tcgcagtaag ccccagatca gcaatcatct
     8581 cgtgatccag agaactgttt cgccccgccg ttgtcagata atcaccggtc atcagagcat
     8641 tggcgcccgc cacaataccc aggggaagca attgacgcag atttttttcc tttccgccgc
     8701 agagccggat gtccctgtcc ggaagcatga aacggaaaac ggcgagggtc atcaggattt
     8761 ccatgggcgg taacgctcgc atatgactca aaggtgtgcc cttgaccgga tgaaggatat
     8821 tcagaggaac ggagtcgaca tcaagttttc tcaaggtttg agccagttca atgcgatgag
     8881 aaatgttttc ccccatgccg atcagagcgc ccgaacaaac cgacaaaccg gcttctttag
     8941 ccgcacagag tgttttgacg ctttcctcgt aatcatgcgt ggtgcagacg gttttaaaaa
     9001 aactccgcga tgtctccagg ttatgatgat aacggaacag cccggccgct ttgagctcca
     9061 tagccgttgc tttgtcgatc attcccagtg aggcacaggg gaaaagacct gtttcgcgga
     9121 cgccctgaat ggctctgatg atttccagcc tttctttttt cgactttaac tgtttgccgc
     9181 tggttacgat gccgaaacat tccgctccgt ttctgaccgc cttttcagca tcggcaatga
     9241 tttgtgcgga ttttttcaga ggatacgagt gtgccgtcgt tttgtaatgc gaggattgcg
     9301 cgcagaacat gcagtcttcc gggcatcgtc cggatttggc gttggtgatt ccgcagaaaa
     9361 taattttctt tcccttgaaa tgttcccgga tttcggaagc agcggccaga accgaccaac
     9421 agaagccgga cgcttcaatg aacagctcca tagcctcggc cggggtgatg aaacgcgatt
     9481 gccggataat tttgtttttt aagtgatcga cgaaattcat tttgactttc tctctttttt
     9541 tacatgaccg agtttggccc cttctttttt cagtatgtta atgagctggt catgataata
     9601 ataaaccgca ataaaaaccg ttacacccag aatcagaata accgtcagaa ggatgtactg
     9661 cccgttgcgg gcgacgttgc cggtaatcga aagcatgatg gtgccgaaaa tgcggccggc
     9721 tgtggagatg ataatgaacg ttccgaccgg tatgcggctg actcccatga tgtagcatag
     9781 ataatccttg ggaaagccgg gaatgagaaa aagcaggaag gagaccagca gacccttgtg
     9841 ttccataaag tgatcaaatt tgtcaaaaac ctctttctta accacatttt ccagaagcgg
     9901 ttccccaaaa aaccgggcca gcatgaaggc aatccatgac ccaattgtta gaccgatggt
     9961 tgaataaaga gttccccaca gcggtccgta aagataaccg ccgatgatgc cggtcagttc
    10021 gccaggaatc ggggcggcaa cgacctgaat gatctgcaca agaataaaaa taaactggtc
    10081 gtaaggatag gaaagaataa aattgaccag cctgtcccgg tctcggaata gatggtgtaa
    10141 atcataatga ataaagatga tggccgacaa ggcaatgaac aaaaataaaa agaaaattct
    10201 gtaagccagg cgtttgtcca tggatggttt tgatccttac aattgcttga tttgaaccgg
    10261 ttggcgattt tttatagcat tattctgctt tcgtcaatcc cattattgga tccatccggc
    10321 ggtttttttg ttcatcttcc ggaaaaatcc gttaaacccg ttgattttaa aaatgccctg
    10381 tgcaaaaaca agtgaaaaat ttttcatcct ggtttatagg gtgttttatg aaaagcgaaa
    10441 cacaaaaagg ggaggaaaat tgtatggcta aaatcccgca gaaactggaa agaaaaaaat
    10501 cggatatcta taaagacgcg ccgatagcga aattcggaga gagaaaaccg gacttttcaa
    10561 caatgggccg gaaaatgaag aatcctcatg cccggtttcg agaggtggtc tgtgtcgagg
    10621 cctgtcgaac gccttacggc cgttcgggcg gtgcgttaaa gaatttttcg gctatggagc
    10681 tcggcgccat ggccattaaa gaagtcttgc ggcgcaccgg gggcaaagtg gccccgtcgg
    10741 atgttgacta tatttttatg ggacaggtag ttcccgccgg ctgcggccag ataccgggtc
    10801 gacaagctac gattcttgcg ggtgtgccgg aatttgttcc gtctataacg gtcaacaagg
    10861 tctgttcgtc cggcatcaaa acagttgatc tggcttttca gatgattctt ttggggcgcg
    10921 cggagatctg tattgcaggc gggcaggaaa gcatgagcaa ctgtcctttt gttctgccgg
    10981 acatgcgctg gggagcgaaa atggctctgc ccaacggacg ggttgtcgat tcaatggttt
    11041 acgacggcct ctgggatgcg ttctataacc ggcatatggc cattcatggc tcggaagttg
    11101 cggatgaatt cggattttcc agacaggagc aggatgaatg ggcgcttaat tcccagatga
    11161 acgcggtacg tgccatgcag gaaggcagac tggatgacga agtttttccg gttgaagtcc
    11221 ggcagggcaa agcaatattg atcatggaca aagacgaggg gccgcgtccg gaaacgacgc
    11281 ttgaggggct ggcgaaactt cctcccgtat ttaatcatac gagtaccgtt accggccggc
    11341 ccggcagtgt aaccgccggg aatgctcccg gcgtcaatga cggcggagat gtctgtctgt
    11401 taatgagcag ggcgaaagct gatgagctgg gattgaagcc tatttttacc ctcgtggatt
    11461 acgctgaagt ttcccagccg accaaggaca tcgccactgt tccggggttg gccatcaaaa
    11521 agattctgga tcagaatgac atgacgcttg atcagatgga ggtcattgaa attaatgaag
    11581 cctttgccgc agtcgcactg gtaagctgcc gttcgatact gggcatgtcc agggaaacaa
    11641 tgtttaaaaa agtcaacgtc aacggcgggg cgattgctta cggtcatccc atcggagcga
    11701 ccggtgctcg gattttaatg acgctgggtt acgaattgag aagacgaggc ggcggctggg
    11761 gcgtctgcgg gatttgttcc gggcacgcac agggcgacgc catgctgatc agggtggact
    11821 gaaatctgtt ccgaaaggct cttgagggaa atctccggga ttatgaaaaa ccaaatcatt
    11881 tttgattgct ttttgctaaa gcggcttttt cttacagtcc ggttttgaaa aaaaatcaac
    11941 atacaaaaga gttgaaaatg atggtaagtt atgtaaaggt tcatgtccaa aaccttaaaa
    12001 tatcagtaaa ttttgttggt caatcatttg caaggggttc ttgatggctg gaaatccgga
    12061 tatgtccatt gttgtgccgg tctatggaag cgatatcacc ctgatgcctc tttattgtcg
    12121 gactgttgct gcggtcagcc cgatttcgga aaatttcgaa tttattctga tcgatgactg
    12181 cggaccggga aaaccatggg agatcatcgg cgccatggcg aagaaggatt cccgggtaat
    12241 cggtctgaaa cttgcgaaga acgttggcca gcacaatgcg attaaagccg gggtggatgt
    12301 ggcgcacgga caatgggtag tcgtcatgga ttgtgatctg caggaccgtc cggaagagat
    12361 tccccgcttc tggggaaaag cgcaggaagg atataatgtt gtgatcggcc gtcgggtgga
    12421 aaggcgcgat gaaaccatca agcgcatatt ttcatatatc ttccacggtc ttttgaaatg
    12481 ggtaacccgc cagaatagtg atgcggcgca gtccaatttc ggcatttatt cccgacaggt
    12541 tattgataaa ataaaaaaat tgcctgaaca atctttctat ctgccgttgc tggtcagacg
    12601 ggcgggcttc gacgtgatac cccttgacgt ggaacacgat aaacggcctt atggaaaatc
    12661 gggctataca ttcagccgaa gattatctct ggctgtggat attcttgctt cctccggcga
    12721 tcatccgttg aaaatgtttt cgttgtttcg atttttcaat gcctccagag ggaaacctct
    12781 ctacgtggtg acagatcaga tccggatgac gtaagaaagc aataccctta ttgagtttcg
    12841 accctgcccg gtcggggtta tgccttcgga caggagcctg atcgtcttta gaaaaatcac
    12901 ggctgaaaat ggacacggtg tggagaaaat gaactgtgaa gaataaaatc gagattcctt
    12961 ttaataagcc gtatatcgct gacaaggaac ttatttatat tgcccgggcg gtgatggaaa
    13021 acaaccatac tgccggagac ggtcccttta caaaaaaatg tcagatctgg ctggaagaaa
    13081 aacttcattg ccgcaaagcg ctcctgacgc attcgtgtac agcggctctg gaaatttcgg
    13141 caatgctggc ggatattcag cctggcgatg aaattatcat gccgtcctat acttttgcat
    13201 cgacagccaa tgcttttgtt ttgcggggcg gaatccctgt atttatcgat atccggcccg
    13261 acacgctcaa tcttgatgag gcgctgattg aggccgctgt gacgccgcgc accagagtca
    13321 ttctgccggt gcattacgcc ggcgttccct gtgagatgga tgttattgcg gatatcgccc
    13381 gtcggcatca gctgttcgtg attgaagacg cggcgcacgg catcctgtgt tcctataaag
    13441 gacggccatt gggaagcatc ggacatttcg gcgcctgctc atttcacgaa acgaaaagca
    13501 tgatttcggg ggaaggcggc gcattgctga tcaatgatga aaagtatgtc gaacgggcgg
    13561 aaatcatccg ggaaaagggc accaaccgca gtcagtttgt tcggaaaggc ttaagtcaat
    13621 acacctgggt tgatgtcggg tcttcctatc ttcccagcga tatcattgcc gcatttcttt
    13681 atgcccagat ggaaaacgtc gatcaaatga tctcccgacg caatgcgatt tatgatgtat
    13741 attggaagca attggagccg ctggaggaaa aagggctgat ccggcttccg actcaacgcg
    13801 acgattgttt ccgcaacggt cacatttttt atgtgatcac cggatcttta agagaaagaa
    13861 tcgacctggc ctcttttttg aaagaccggg ggatctcggc cttttttcat tatgtgccgc
    13921 tgcacagttc tccggccggg aaagtatacg caaggacgtg cggcgccttg gaggtaacga
    13981 ccgcgatcag caactgtctt ttgcgtttgc ccctttacta cgcgatgacg gaagaagatg
    14041 tccaggctgt tgtcagggcc atcatggatt tttatcgggt ccgttgatgc cggatatgct
    14101 gcagggtaaa tgaatttcta ttgtgaaaaa cacgaggggg aaaatccatt ctatggacga
    14161 aagggaagaa aacggaagca accccgttgc tgcgggcaat gacgcccctc aacatccttt
    14221 acgggcgtcg tctttttttg ctttttctga aaaactttca gcatcagtca atctgaggtg
    14281 gctgatgatc tcccttcttc cggttctgat tttactcaag tatccggtgg accggattga
    14341 ttatgatgtg tggtggcaga tggcgcacgg gcaatattat ctcacgcacc atacactgaa
    14401 aatggatctc tcaatgtttt cctggacgcc gacggatcca acctggattt ataatacctg
    14461 tctgggaagc atcgccgttt atctgtttta ccattgggcg gagggattcg gcttgtggct
    14521 ttttcaatgg cttatattca gcggcgtttt tctttctttt tacctttttc tgcgatcaat
    14581 ccatcagcgt ttggatatca acagcgcgac ggtgatcgcc gccgttgcca tggcctgttc
    14641 catctcctgt cgttattaca agcccgaatt gttgtccgca ctcttattct gttggacggc
    14701 acatattttt tttcatatca agatcagggg caaccggtat cttttttatc ttttcccgcc
    14761 gattttcgct ttttgggtca atcttcacgg agcctttatt gtcggtctgg cgtttctcgg
    14821 cctggcgttt gccggcgagt ttctcaatag aatttttttc ccgaaaaaat cgttgaagac
    14881 ggaagatctg atgcatttcg gcctggccct tattctttcc ggagcggcca cgttgctcaa
    14941 tccttatggg attgactatt tgaagagcct tgtcccgaca atcatgaacg ctgtcggcgt
    15001 gggagattat tccggcccgt atgacaaatt tgttctggcc tatgccagcc tctggcctta
    15061 tttaaggaaa atggggcctg agttattcgg tgtcggaatg acggcctgga tattgacgct
    15121 gatgatgatg agcattgcgt ttcttgttgc ctatgatctg atgaaacata aatcgtgtga
    15181 ctttgcttta tgcatcgtca gtctggcttt gtattggaaa ggcatggaaa caagtcgggc
    15241 aacctatttt ttcccgattg tctttttctt tatcttttat tatctgctgg tttcccgttt
    15301 gcagatgacc aaattcggca gcaagacgac ggtcttttcg atcatggttt tcattttctt
    15361 tttcggcatg gtttcttttt ttaacataaa atacggggcg gacgataaat ggttcggaag
    15421 ggatcttgac ggttacgtgc cggttgagga agttgaattt ctgaaaaaat ataaactgcc
    15481 gggaccgata ttcaacgatt acgttatcgg cggatatttg ttgtggaagc tttatcctga
    15541 ttacaaggtg tttattgatc cgcgcggcgg cctttacaga aatcaggtat ttcccgatta
    15601 tatggaattc accatcagac atgtcagccg ggatgatatc cgtcgattca gagagaaata
    15661 tccttttaac attgccatcc ttcattacag acagatggcg cttatttttg attttttaaa
    15721 agccggagac gactggcgtt tattatattt tgaaaaaaat gcggccatcc tgatacatca
    15781 atcgctgttg cccctcattg agccgatagc gggcaacatc agtctcagcc cgatccgttt
    15841 caaacatgtc cggaatccgg aaattctgac aaatgttttt aacttttatg tccggcttga
    15901 tccaagggcg ggtagataca tttattatgt cttcaagaga aatgtaagtg acggtttctt
    15961 catgaagaag gaaattttga ccgccatgga ttccaatatc cgtttaaagg aaaaggcgtt
    16021 tcaggaaagg cccgatttgt ttttccatga atgaaggaaa aataatttga gcaatgaaat
    16081 atttctagac ttatgtcata aacgggtcaa tagtgataaa gaaccgattg agtcatggaa
    16141 aggatccctg cgaggtcatc atgagttcgg atgcattcaa gatgtttatt tcggaaattg
    16201 atcaggaacg ttttggtatt aagacagcca gagttgttga tatgacggcg gatcgccttc
    16261 cctctgtcct ggatttctgc gtcagccatg cggtaaaatt attaattgca cgttgcagta
    16321 tatctgatct tggcgcggcg caatccatgg aaaagcaagg cttccttctg atggataccc
    16381 tcgtttatta tacatttgat cttctgaggc ggccggtttc ttcttcagat gatgatgttc
    16441 atttcaggcc gatccggcgg ggagaagaaa acgttgttga gcgggtagcg atagagtcgt
    16501 ttcgcggcta tttcggacat tatcatgccg acccaagact ggaccgtgat aaatgcgatg
    16561 acgtttatgt ggactgggca agaaaagcct gcgttgcaaa aggttccgat gaaaatttta
    16621 tggtagccga gattcaaggc agaatcgtag cttttggcgt tttccgcatg aataatccgg
    16681 atgagggcga attgtttctg ggagggattc atcccgattt ccgggggcag ggcatctatc
    16741 agtcattttt gcacagagcc aaggcgtggt gcttgtcaaa agacgccagg agaatggtta
    16801 tttccaccca gctcaataat attgccgtgc aaaaaattct ggtcaaattc ggattcgaaa
    16861 tcaccagagg gtgctataca ttccataagt ggtttgatta gcgttttgca gtctggtgtt
    16921 gaaagaactt ataaagagaa agaacaataa ttttttctca ttacctggag tcgcatgggc
    16981 gaaaaggaaa taatttccga taacgattta ttgaagactc aaagtcggaa aagcaaccgc
    17041 cgaccatcga taacttcttg tctggttgcc gtttctgaaa gactggctgc ttcccccggt
    17101 ctgaaatgga tgacagctat cttagtgccc ctgctgattt ttctggcgta tccgctgccg
    17161 cgcggcgatt acgatttatg gtggcagatg gctctgggaa aatattattt aacccatcat
    17221 accctggtga tcgatcattc cattttttcc tggacgccgg cggattccgg ctggatttat
    17281 aatacgtttc ttggaagcat tattctttac ctcatgtatc attgcatggg cggcttcggt
    17341 ctatggttgt ttcagtggct gatctttctc ggaatttttc tttcttttta ccttttcctg
    17401 cgatcgacag gccaacgact ggatgttaca gcgttaaccg ttattgccgc catcggnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggc ttgacgagag tgtccctgtt
    17581 gcagaagtgg cgtttttgaa gaagtataaa ctcgaagggc cgatatttaa cgattacctg
    17641 ctcggcggtt atctgatctg ggcgctttat cccgattata aggtttttat tgacccgcgt
    17701 cttgtgccct atcataaaca ggttgcacct gattattggc aactggaacg catgctgcca
    17761 acccctgagg acatgaggcg ttttaatcaa cgctatcctt ttaagacagc catcattcat
    17821 tacaacagac attttttaat tgaagacttt ttaaaggcgg gatggaaact tgtctatttc
    17881 gagaaaaatg ccgctgtgct ggttcatgaa tcaatgctgg ccgccgttcc gcccgaagtg
    17941 caatgggttg acctggggcc ctcgcgtttc aggaatgtga aggatccggt ggttttgctg
    18001 aatgtcttca atatttacgt gaacgtatat cccgaggcaa gccgggttat ttatgatatt
    18061 tacagaagca atgtcagcac atggtacgaa ccgagagagc agcatctccg cgcgatggaa
    18121 gaggatatgc ggaaaatgca atcactccag cgtaaagcca aatcgtaagc actcctttga
    18181 cccgaccaac cggagtccga ttccaatctt attcgctgtc ttcaataaaa taatacttgt
    18241 ttttataacg catattattc atgaaattag cggatgaatc caccggttct ttaagatctg
    18301 caaccttgct gcccttggat gaaccgaatt taaaaggcag taaaccaaaa atcatatcgg
    18361 tcacgatatg aaggggcatt gtggctttca acgaggccag gtaaacagag acttcctgtt
    18421 cataactcaa cccccggtaa gatgtattca gaccgtctcc cagcgtatag gagttcatgt
    18481 ttttactttg cccgggtctg attttaaatg atttactgat catataaccc agcgcgaggt
    18541 cgatatcgat atctttccat tgaattccca taccggcgcc gaaatagcgc atggagggaa
    18601 gggcgtacat aagatcgtaa tattcatcaa tagttgaact cgtacggttt tcgtaaccgg
    18661 cccgcaattg gagccaatcc agcaactggt attcaacacc cacgccccag ttcagcgtat
    18721 ccttgaattc tctggccact ttcatattgt atgatcctcc gctgtaaccc atgaatttgg
    18781 ccatctgaag aaattgaacc ttttgatcaa atttgagatc gttttccttg accgcggacc
    18841 actccgccca gtgcacttcg cctaaaagag acagtcgctt tgtcggtttg agtttgacgc
    18901 cgacattgac tatttgagga aattccagag tcgtcgagac ggttcccttt tgttcatctg
    18961 tggccgtgta aggcaggtca aaaatgatcg acgaaatctg catgacagcg gtactgcccg
    19021 accaggccac cattctttgc cagtcttcac tgtatttcag actgaatttg ccggatacat
    19081 ttgatttaac ggcactctga taacaaagtc caagcgctac ccagtcaaag ggttcccaca
    19141 atactcccag attataagag ggagaaaaat catctcttaa gctcaatgat aaatcgccta
    19201 tattatcata aaccccgacg ccgccggcga aaagcggcag gggaaccgtg acatcgaaaa
    19261 gcggattggc catgccctgt gttgcgtccc ccagaatttt cgttatgttg acgatttcat
    19321 tgggacctct cattttcagt tccagtccca tggctgtctg cccgatgccg aacgaggcgc
    19381 ccacggccag attgttgttg atccggtagc tgatggccgg agcggcataa atcagatgct
    19441 gcagatacac ggattttgct ccgtacatcg ccggatcatc gtcgccatat tcccatccac
    19501 cgccgtactg ggcataggtc gtgtatccaa acgtccattt ggagccgggc tttctgtagg
    19561 ataagccgaa tatcggaccc ataacggccg gcgccgtgtc gttcagaatg ggaatgaaca
    19621 ttctgcccgt gctggtgctg ctggatcttc cggcaagcgg atcaacaatg acatttccct
    19681 gataatcatg aaaggcctga tggttggggt cggattcaaa tgtccctgac attttgagaa
    19741 ccgcaggaat gagacccgca ctgatgtaac tgccgtcccc cataaaagag agtccggcag
    19801 ggttataatg aatagatgcg gtccccgggg gatcagccgt cacgttgttg gccagtgaaa
    19861 tggctttggt gtcaatggcc atggattcat tgaaagcgcc ttctgcctgg tgcaggccga
    19921 aaaataaaac cgccgccaat gtaatgaaca aagaatagat cggcacatac aaaagcaatt
    19981 tattcgaatt taaaattttc cttcggaaaa aagataatac cggtaaagat aatgataaat
    20041 aggtaatcaa ccttttgatc tttgaaagta ttgtcatcat ccctccccca ttactatgtt
    20101 gtaagataaa acgtaaaaaa acattttttt ctgatcgtta aaaagttctt atagagattc
    20161 tcctaaaatt catacggtaa ccgcaaagat aaagacacgt cgggatcgtt attggtcagc
    20221 cccatgctga cagttgcata tattgatctt gaaggagtta ttctccaacc ggttcccacg
    20281 ttaaatgatg aagagaggga actgcctgtt tcagtggtgc cggtgttgga tttgtatttg
    20341 ctgccgaagg aataactgaa ttgcgcgctc atattcaaag aagcctgata ggacagggca
    20401 taaccgaatc ccatggacac tccgatgctg cttcccggct cgactttttc cagatatatt
    20461 ttcgatgccg ggtcagaagg atcctcatac agaatttgag acaggccgct ttcgggtatg
    20521 ccgtaggtgt agtttaaatt tccgaaggcg accagcgggt ccaacacctt gctgaacgaa
    20581 acccccgcgc caattccgta atagccggaa ccggttgcca gttcgtcttc ataattaatt
    20641 tcgaaggggc tgcttcctgt ggggagggac aaactcaatg ataatatggt tgtggggatg
    20701 cgacctccgg ctttaaacgg ctgccacgag aatccgatag agatatctcc cagatccgtt
    20761 acgtcaagag attcatcggt gcccacttta ttgtatttgt aaacgaaagg aaaattagaa
    20821 ctgacggtga gattattaaa aaaagcgtat tcgagagcaa gggtattggt cagattatga
    20881 ttgactctgc gcaaaaccgt tgttgattca tcaatggcat cgccggagta atacgcatag
    20941 ttgaacgtat aggacagccc gacagtgcct ttcttcaaaa gagaatactg ccggcctgcg
    21001 gcagaaagta tgtcgtcaat ggtctggctt ttttcttctt cggggatttc cagctttttg
    21061 cgcgcttcgt tttctccccg taatttttga atttcctctt taagttcttt taactcctgc
    21121 tcgatgctct ttgccgatcc ctcctgagtc gtgactggct tttgcggttc ggcaacggga
    21181 ggagcttcga ccgcttttgt ttcttgaacg tccgttgttt ctttcggagc tttgactgcc
    21241 tcctgatcag cggccgcatt tgcatcgtcc gcgctgacac cgcttaacgc aagctgaatc
    21301 ccctcaagag gttcattggc cataaccggc agggccgcaa gaagggtaaa ggttaaaaaa
    21361 aataataatg acaaaacaat gacaaatttt cttttcatca atatcctttg ggttaagtag
    21421 tttggttacg tgataatata cttttaaaat aaaatatgcc gagaaacgcc atcactactt
    21481 ttcttcatgt catttatcga atgcataaaa atattcacct ttttcttaaa aaacatattg
    21541 aaaagcgaaa aaagctcgtc aaaatttttt aacaactcct ccccgtttct taaatcacac
    21601 agttaccttt ttgttaaaag ccggtgttta aagaaaaaaa gggcttttga ttgaagcaat
    21661 aaacttattt ttacaaagtg gcgaaataat atacaagcat gtctcggttg tcaaggagaa
    21721 acagcatttt atgaatgaaa atataaacgt ttttttaagc acttaagccc tatagaacaa
    21781 tttttttaca gggatgcccg tactttgaaa tcgctaatga aaacaacagc gctttggagc
    21841 gcttcgtttc tttaaatatt ggatattaaa gagcattatt tttttattac gtcaataatt
    21901 gtgatttccg gaggtgcaag gaaacgaacg ggcggccccc atgtgcccgc tcccttgctt
    21961 atataaacgg atttgttatc ggataattga tgatatccgc tgttttttgg gaaaaaaatc
    22021 ctcgtcagga acataaatgg gaaaatttgt cccccatggg tatggccgga aagctgcaag
    22081 tcgaaatttt tatcttctct gatggtcggc tggtgtttta acaataaaat aaactcttta
    22141 ctttttttaa ccgggaacag attggataag ctggggtttt tttctgaaac accgtaaccg
    22201 cgtccggtcg gatcatccag cccgatcata tgtattccgg ctattttttt gctctcatct
    22261 cttagtattt caaaaccggc actttttgta aatgccagag atttttcaat tcccgcgtaa
    22321 tattcatgat tgcccatgat ggcgtatttt ccaaactttg gttttatttc catcattctt
    22381 tttgcttcag acgcgatatt gtccagttct ccgtccagca aatctcccgt ggaaaccaga
    22441 atgtccggtt ttgcctcccg taccttttca aggatgtttt gcaggcgttt acccctgaca
    22501 atcagcccga tgtgaacgtc ggatatctgc acaatacgta atgtttcatg cccgggcggt
    22561 aatttttcag tatggatttc cagctttttg acttgtatgt ttttcgcgtc gaagaacccg
    22621 tacaccacaa aagacaaaga aaggaaaaga gccgtgaaaa aaagaatgtt ttttaaagag
    22681 tcgcctcccg gggtctgaaa tattattttt aataacggcc ggatcagatc aaaggaaaca
    22741 tgaataacga aaaataaaaa aacaaaagcc atccacaggt acccgcaata agctattgat
    22801 cgggccagcg tttcccaatg aattttttcc gccagtttta tgataatcgg agccagaata
    22861 agaaaaatca ttgaaataat tatgatgatt tcaattttgc cggaaaagtg gaagagagtt
    22921 tttgccttca gaaaagcgta aaaattaatg ccgccgtaaa gacttaaaaa aatcagtagg
    22981 aaaagaatca tggtctcgac cttgcttgca aaaagatgta aaaacttgac aatcaataac
    23041 ccttaagata agagtttgac ctctttataa gaggctgatt cattccggat gccgatgtag
    23101 ctcagccggt agagcaactg attcgtaatc agtaggtcac cagttcgatt ctggtcatcg
    23161 gctccagaga atcaatgagc cccgtcatcg ggaatcatct tttttaacta cacagaattt
    23221 taaaatccgt caacataaca aaagacttga tcctctgtgc atggactcat aaaccggagg
    23281 gagataaacc gctgtaaagg gttaactggt atgacgttga taccgataat tttcatgcga
    23341 ttaagataga gaaaaaatca tctgatgatt ttaaaaattc tgtatgcgat atatttcaat
    23401 ttatccagag atttggtggc ccgaagaaca ttccgaatga ctttcggccg caaataaaaa
    23461 ttaataaatg ccagcgcgaa cagccaggtg aattttttac gagacatgcc ttcaggcaca
    23521 tagggtttca tgtcctgtcc cattaagtcc caatgatgaa actcaaaatc tttttgggtc
    23581 tgaagttgat tgaaaatttc cgtaccgggc gtgggatgaa acgggaagaa gttggcgcca
    23641 tatagaggaa gctttccggc gaattttatg gtttttcgga tgtcgtcttc attctcagca
    23701 ggataaccga tgatgaaatt gccgataatg tttatgcgtg ttttgctgtt gatgagtttg
    23761 attttttctt caatgagttc aatcgtcagc ttttttttca tatgatcgag aatccgctgg
    23821 gatccggatt cgatacccac ggttaataaa tagcagccgg atttttccat caggtccagc
    23881 acctgttcgt caaccaggtc cagacgaatg ccgaaacaag accaccggat gtccaggttt
    23941 tcagcaagga tcttttcaca caactccatg accagatctt tccttgccag aaaattatcg
    24001 tcaatgaaac atacctctct gatgccgaag cgcgattgca gtagcttgat ttgatggatc
    24061 atgtaggaaa cgctgtgggc cctgatctta tggccggtga ttgcgcggca ggaacagaag
    24121 gtgcagtggt aagggcatcc gcgggtcata ataagcggcg cgacgatttt tgaccgggca
    24181 aagtaagatg tctggaaagg ataatggcag ggattgatta aatcccaatc cggcgaaccg
    24241 agggcgtcaa gattttcgat gaacaaggga ggattgcacc tgatggctcc gctttctctc
    24301 cagatcaatc cgggaatctg atcttttgca atttgatccg gctgagacag gtgcgtgatt
    24361 aaatcggtca gtcctttttc cgcttcacct ctgatggcaa aatcggcttc ttcgaaatat
    24421 tgcagcgtct gttcgggaaa cgt
//
LOCUS       DAOSME010000032        23565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2712_length_23565_cov_22.856827, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000032 DAOSME010000000
VERSION     DAOSME010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23565
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2712_length_23565_cov_22.856827"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PLG98_06365"
     CDS             <1..1094
                     /locus_tag="PLG98_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOE32914.1"
                     /translation="EPEIPNPRWLKVKNLACGLCGTDIHFMFMEMDPKCFPAATPGIA
                     RKYLGHELLGEVMETGNEVEGLKKGDRVCLRIDWPSCFQMEMDPLCPQCAQGEYMLCQ
                     NLGARELPLTDVGGGFSPRMVMHRTQPFKVPDNLTNDEALLLEPMACAVHAVLKHSPT
                     AGNRVLVVGCGTIGLLTIATVKALAKDAEVTALARYPFQADMARALGADEVITGREDP
                     YRRIAKQTGAKYFEGYFGNQILLGGYDVIYDSIGNDGSLNNALRWAKSRGTIVIVGVN
                     FKPGKIDYSPMWHQELHVTGINCHILQEGSLPSSFDIAAQLIADKRLPVSRLITHRFP
                     MDRWRDAVKTFLSKGDRGAIKIVLEHPSA"
     gene            1247..3394
                     /locus_tag="PLG98_06370"
     CDS             1247..3394
                     /locus_tag="PLG98_06370"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOE32915.1"
                     /translation="MEDKESNQIPTDRSWQERFFIYTGILASFVALYLVKNHNYVLFH
                     SLVEVFSIIIAAAIFALAWNARRYFDNGYILFIGISFLFVGFIDLIHTLAYKGMGVYP
                     GYNSDLPTQLWIAARWLQALAFFAATFFLDRKLNRPLVVLAGGAVLSALLLLSIFYWN
                     IFPSCFVEGTGLTPFKIASEYGISLILLISIVPLTKKKDKLHPRVRQLIIASILSTVA
                     SEMSFTLYTDAYGFFNALGHFFKVIAFIFIYKAVVSIGLKQPYDLLFRDLKIRQDELR
                     RSEERYHSLFKNMLEGMAYCKMEFDADGRPVDFIYLAVNDAFEKLTGLKNVEGKRITE
                     IMPSISKENPDFLELYGRVATTGQPEKRELLLKPLDLWLYLSVYSIEKGSFVSVFENI
                     TERKQVEKDYRSIFENVQEGVFRSTPDGKIILANEAMAKIFGYDSPAEIMADITDVAA
                     QLYVHPEERASVKKIIAEQNAIQNHEVQFHRKDGGAFWGSATMKAIRNGKDQILYYEG
                     IIEDITERKLSIERLRKTLGGAVQAIASLVETRDPYTAGHQRRVADLARAIATEMGLS
                     GDQIDGIRIAGTIHDLGKISVPSEILTIPRKLTALEYSLIKNHPQSGYDILKDIEFPW
                     PIARMIVEHHERINGSGYPNGLRGEDLLLESKILAVADVVEAMATHRPYRPALGIDAA
                     LAEITANRGILYDPEVVDACLRLFRDKGYQISH"
     gene            3540..4706
                     /locus_tag="PLG98_06375"
     CDS             3540..4706
                     /locus_tag="PLG98_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE32916.1"
                     /translation="MDITGEKMTSGEFRKLVETARFGPFIFQIGVDYHEAGMLLQRVS
                     DAQSRFLSAPLSQVANRLEQEVLVSSIFSTNTIEGGTLTEEETKDALDINPTQAQAEE
                     QRRAVNIKAAYDLAQNAAQDLRWRLSVDFIKHLHAAITDGISHAYSRPGLIRNNPKNV
                     VTRVGDTAHGGTYKPPQYEGDIELLLHHLAAWHNEMADAEIPALIRAPLTHYYFELIH
                     PFWDGNGRVGRVLEATVLHGAGFRYAPFAIARYYLERIDRYFTLFNLCRKQADQKKAY
                     TNTPFALFHLEGMLIGLNRLHDRVNAIVKQLLFETRIRHLRDTKEINLRQYVILTQMM
                     ERDRPLKFDELRRAPWYLVLYAKLGEKTKQRDLSGLRDQALLVVDEKGLVWPGL"
     gene            complement(4867..6489)
                     /locus_tag="PLG98_06380"
     CDS             complement(4867..6489)
                     /locus_tag="PLG98_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOE32917.1"
                     /translation="MVKTSISHPLRIDEVKVPNGAGIIGMTLCPGKKINSAQSGLWNR
                     DLDIDLTAIQAWGARALVNLMEEHEYELLRVPDYVAQVRSCPMEYFHLPIVDGHRPDG
                     RFMVLWKSAGPKLRQILLDGGKILIHCRGGLGRTGTVAAQLLVELGMPHEKAIQAVRD
                     ARHGTIETQDQEKYVYSCQPVNRRLNVNHFAGCLLGGAVGDALGAAVEFDSIGAIRRQ
                     YGENGIENYAPCYGRKGAITDDTQMTLFTAEGLLRAWCRKKQGAVPAEYPEMVHRSYL
                     RWLSTQGARSAHEDFDLAQNGWLLTVADLHHRRAPGNTCLSALQSAKMGTPEKPINNS
                     KGCGGVMRAAPVGLFAAKAMQSEGNHGEVWKEIFDLGCRAAAITHGHPSGYLPAGCLA
                     TIIYGIISGLTIEESIHETLPILAMAEGYEETLNAIKLALNLWKKKTAKPAPETIEKM
                     GGGWVGEEALAISLYCALAAEGDFAKGVRLAVNHTGDSDSTGSITGNILGAMQGKAAI
                     PAPWLEELELKHVIEEIAGDLFVQNEALRKYK"
     gene            complement(6654..10019)
                     /locus_tag="PLG98_06385"
     CDS             complement(6654..10019)
                     /locus_tag="PLG98_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HOE32918.1"
                     /translation="MAITNHERVGKAMELLKDGLVPFVERELKAQYAQQWIEEARTSV
                     SDIQAKLFVTKDKPQWDIAAILSVIWSKWDTVFRKTLGRNERSIVSELRDVRNDWAHQ
                     KPFSGDDADRALDSAERLLSAVSAPQADEVRKIKMELRRTIYDEQVRTEKRRSAGAAI
                     ESAVSTSLKPWREVVTPHKDVASGRYSQAEFAADLWQVHMNEGTDEYRDPSEFFRRTY
                     LTESLKAMLVNSVRRLSGKGGDPVIQLQTNFGGGKTHSMMALYHLFSGIAPTDLAGID
                     AVMQTADASKLPSVRRVVLVGNKISPGNPVKKSDGTVIKTLWGELAWQLGHAAGGIKE
                     AKKAFARVAADDEKATSPGDVLRELFKEYGPCLILIDEWVAYARQLHEQSDLPAGSFE
                     TQFTFAQVLTESAKLSKNCLLLISLPASDGGPSPHTQADDVEVGGQRGRDALERLRNV
                     VGRIESSWRPASAEEGFEIVRRRLFQPMTNSAQFTDRDVVARAFADFYKAQQSEFPPE
                     CREADYEKRMKAAYPIHPEVFDRLYTDWSTLVKFQRTRGVLRLMAAVIHSLWEKGDRN
                     PLILPANIAMDDPRVQSELTRYLTDNWVPIIEKDVDGASSLPLRLDGELSATFGKYSA
                     CRRVARTIYLGSAPLTKAAHRGIEDRRVKLGCVMPGESPSVFGDALRRLAGSATYLYQ
                     DGPRYWYSMQPTVLKLAEDRSEQIKRDPDKVAQEIESRLRKDLEKKGEFSRVHPLPKT
                     GQDVPDDLDARLVVLGIDYPYTKGDVSEAQKAAQSILELRGTSPRHYRNALVFLAADK
                     TRLQDLEEAVCKYLAWESIVAEKVQLNLDPQQVKQAENQKNAADTVVAARLPETYQWL
                     LTPEQKTPQDEINWQAIRVTGQDALAVRAGKKLKNDMLLNPAYAASLLRMDLDKIPLW
                     RGNHVSVKQLADDFASYTYLPRLKDSSVLISAIRDGVALLTWMQDSFAFADSFDEAAG
                     RYRGLRCGQQISLTDSDTSGLLVKPDIAVLQIDAEKAKAAGEKPREGGQEATGTKKEE
                     GKKEEIDTTPKAVQPKRFHGTVTLDATRVGRDAGRIAEEVISHLAGLVNAKVTVTLEI
                     EAEIPTGAPDQVVRTVTENSRTLKFTSQGFEKE"
     gene            complement(10031..13708)
                     /locus_tag="PLG98_06390"
     CDS             complement(10031..13708)
                     /locus_tag="PLG98_06390"
                     /inference="COORDINATES: protein motif:HMM:NF020302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
                     /protein_id="HOE32919.1"
                     /translation="MRKDLEKRTYMRSDCVVFRKTKELFGGLSNMAAGFSLKVNKTYI
                     PTSEALYQACRFPHRPEIQQLIVQQASPMTAKMKSKPHRNDSRPDWDVVRIKIMRWCL
                     RVKLAQNWDEFGKLLRATGDRAIVEESRKDDFWGAKTVDDKTLVGGNVLGRLLMELRE
                     ELKGVNSESLKQVLPLDIPDFRFFGEPVGIVADKYAKMKPKAFAEPKVCVESVDLFGT
                     SHTASEVHDKLSGPSVEQNIKNETSEKIQGGKPSDIRKKTNNIMTYKKKLIEVALPLE
                     TINVASALEKQPFTRRHPRSIHIWWARRPLAAARAVIFAQMVDDPSAHPDLFPTEKAQ
                     DKERKRLFKIIEALVKWENTTNEKVMQQARDEIWTSWRRTCAENADHPKAKELFDRYK
                     LPAFHDPFAGGGALPLEAQRLGLEAYASDLNPVAVLICKAMIEIPPMFAGKPPVNPEW
                     QSKTPEEKAMRTWSGAQGLAEDVRYYGKWMSDNAEKRISHLYPKIEVTKEMLQERPDL
                     KKYLGQKLKVIAWLWARTVKSPNPAFAHVDVPLVSTFMLSTKAGKEAYIEPVIENDGY
                     RFTVKVGKPKDAQATKNGTKVGRGGHFICLMSGSPIPVTYIREQGIAGTFGKRMMAIV
                     AEVPNGRVYLAPDPVSEKAAQDAMPSWIPEQEINHNPRDIRTQLYGLMHYGDIFTPRQ
                     LVALTTFSDMVQESRELLIHDALAAGLPGDGKTLDAGNGVIRYADAVMVYLGLSISRW
                     SDLSNSLCSWNSTNQNVRALFARQAIPMTWDFVELSPFSKVGSWFSTIKSSVEATVSL
                     APKQFGSAIQADAATQTISKDKCISTDPPYYDNIGYADLSDFFYVWLRRSLKPICPDL
                     FATLAVPKAEELVATPYRHGSKEKAEVFFLKGMTQAMNRIAEMAHPVLPVTIYYAFKQ
                     SESNGAYGTASTAWEIFLEAVIRAGFAISGTWPMRTEREARSVGLGTNALASSIILVC
                     RKCNDNVQTITRRDLISALKAELPEALVHLQRGNIAPVDLAQAAIGPGMAVFTRYSKV
                     LDAEGKSLTVRDALSLINQILDETLTEQEGDFDADSRWALAWFEQFGFNEGEYGVAEI
                     LSKAKNTSVEGLVDAGFLNSKSGKVRIMKPCELPADWDPEKDKRLTTWEMVHHLIRVL
                     ESGGEAEAAKIVAKLGSKAETARELCYRLYTLCERKKRAAEALSYNALVQSWPEISRL
                     ASDSHEIEESQKSQSELFEND"
     gene            complement(13712..14755)
                     /locus_tag="PLG98_06395"
     CDS             complement(13712..14755)
                     /locus_tag="PLG98_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32920.1"
                     /translation="MSERSDLLKSISTTIADYREGEMPRPTPEHVERWISQFARDVQL
                     PLLKEVDHVFGQLYFAKDLVIDIFNKRIKNENIAGKNPRTFWKRVNFLRIQKDGHSQE
                     DLLALFDVCLRKQYGIDINSCGAEDGAFIYLDDILFSGNRIGSDLSLWIQQAAPAKAI
                     VHIFVFIVHSLGEWQMMEKLKDETIRAGKKIDFHLWRSMTLENRKSYRNSSEVLWPAT
                     ITADANLIAYIDQEKKFPFEFRKTGGSLKNNCFSSEEGRQLLEQEFLLAGMKIRALCK
                     NPSNAMRPLGFSAFGLGFGSLIVTYRNCPNNCPLALWWGDPESPRASPLSKWYPLVPR
                     KTYGRAIDFDVVW"
     gene            complement(14858..16189)
                     /locus_tag="PLG98_06400"
     CDS             complement(14858..16189)
                     /locus_tag="PLG98_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32921.1"
                     /translation="MAQLFIPALPIVVTPPQGVSSLPMIEINKNSVMTKHKQIRLHAG
                     SYKWQTANCSFGNFTQHLGFTAWEFFHAAKKTELASSFTNAFADRRAFLFAAEHEDTF
                     IWNTGNLRKTDNSVFALEDFAMAGLAGRVGEAIAYLTMIKWGYVFWDRCATVWERAAR
                     SANITHSEQLRTVQYLSAKIASGRPQNEPDFIFEKRNGDVALMEAKGSFVNPTKDNPS
                     AKADLRQALTQLAAWSSVIVPTPKKSYGIATYFREEHDNNRDPSLIAYVDPPGRKDEN
                     IRPVELPRDLVRRCNYGTWLVGMGLVSAGRALCEGKKKTIQMIELPTVEVNGQRFVYA
                     VMGFKSLPGHDNQLSYPLSWINGVFSRWHQSSGIFVIGIEQGVMYLITEMLHRSKTET
                     MKRNGGHDVTGFSHHNNEEQSWSVMPDGSFLGILDYDTLDAGFKGMEKFNL"
     gene            complement(16238..17932)
                     /locus_tag="PLG98_06405"
     CDS             complement(16238..17932)
                     /locus_tag="PLG98_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOE32922.1"
                     /translation="MSLFEDTNPKPLKELLAEIHSLTTVLPDFQRDFVWEPYATQELI
                     ISIANNYPAGSLLRVRDKKRVFAAREFEGAPTLNGKKHTFLVLDGQQRLTSLYQAFYG
                     VGEHCYYLDLRKLLDGNDFEEAIFHARATTKWVKEHEYFDIQAQQLILPLSVLKNGSG
                     GFLKWLLKATNPMPPEERTKMLDALTKINDQWIMKIDDYHFPVVTLSDETEPDALCTI
                     FETLNRTGVKLSVFELLTARFWPQNINLRNLWEKACGENAIIDDFEVDPYYVLQAISL
                     ACRKAPSCKRSDVLNLKATDITEWWDKVIVGLKTGLEILRDNCKVIQPKWLPYQTMIA
                     PLAAVLAKVGIPKTAIAGAQREKLKRWFWCAVFGQAYESAPNSKSAKDVLELVDWLGA
                     GALPESVDSLKFDPKSLRDITPRQRAIYRGSICLMLTNGTKDFHTQGIITSKLMNDEG
                     IDDHHIFPADYLEKVKGIKTAKNRDCILNRTLIDRTTNQIINNRAPSLYLEDIRKTPG
                     FPFDDVLKSHWLPTGADSPLFKDDYEAFLRWREQRLWQEIKRVTDIKEASDLESET"
     gene            complement(18030..18482)
                     /locus_tag="PLG98_06410"
     CDS             complement(18030..18482)
                     /locus_tag="PLG98_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOE32923.1"
                     /translation="MELKFRIDVIRLIEAHFALNRQYKWVKDEMVEFQQSIEIEHKSV
                     DNILGVVVRFYSDSEKQPFRFSVAWEGAFVFEKLPAKELLERIAHVNCASIIFPYVRE
                     SIADLTRRASIVPLNLQPMNFVALYEEKQKAATLAAPKKPVKKSKAKK"
     gene            complement(18472..18999)
                     /locus_tag="PLG98_06415"
     CDS             complement(18472..18999)
                     /locus_tag="PLG98_06415"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOE32924.1"
                     /translation="MKSQKCPICGAGALKKTIKTQVFNYKDETKEIPDYVTYECPECG
                     EAIVDSETLKTSGKILKDFQREVDGLLTGAQIKSIRLKLGLKQEQLANIIGGGAKSVA
                     RYETGQISQSKGMDNLLRILDAYPQTLNVIRKNDAHCISKSKITYIKDLQEKIGYQVR
                     EARYTSDEEEMAYGT"
     gene            complement(19001..19345)
                     /locus_tag="PLG98_06420"
     CDS             complement(19001..19345)
                     /locus_tag="PLG98_06420"
                     /inference="COORDINATES: protein motif:HMM:NF027059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsR family
                     toxin"
                     /protein_id="HOE32925.1"
                     /translation="MIPKVREKRKPHYVLSDFKVLFRNESTRVITQIAHKGAVAEGYM
                     TIEDIEGVIDRLCSEHFYKSMTSYANHKVWQDVYKITDNEKKLYIKLQFSLDVPKKAV
                     LIQMKKDEGSDD"
     gene            complement(19390..22962)
                     /locus_tag="PLG98_06425"
     CDS             complement(19390..22962)
                     /locus_tag="PLG98_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE32926.1"
                     /translation="MIRLEDIQPNAAISGIIPNGQITVVSVQWYGSEAMEITYKTPDG
                     KLANELLYRHDEPRLELVEKGRPWSFDGDGAMFRLASEARRIRLAYLFDPLLAIHTSI
                     VEPLPHQITAVYESMLPRQPLRYLLADDPGAGKTIMAGLLIKELIARGDLQRCLIVCP
                     GSLAEQWQDELWSRFHIPFEILTNDKLEAARTGNWFMETDLVISRLDKLARNEDVQLK
                     LQAPDCRWDLVVCDEAHKMSATVFGGDTKYTKRYRLGQLLSTVTRHFLLMTATPHNGK
                     ETDFQLFMALLDGDRFEGRFRDGVHVQDVSDLMRRMVKENLLKFDGTPLFPQRIAYTV
                     PYKLSDAEAQLYKAVTDYVREEFNRAEALENDKRSGTVGFALTILQRRLASSPEAIYQ
                     SLRRRRERLESRLRELELLHRGGQAAPLIDPALRTLDAEDVEDLEDAPDNEVQEAEEE
                     ILDQATAARSIVELKAEIETLKNLESLALIIRRSGADAKWCELSSLLGAIFMTPGCVA
                     ESKPPYGAGQIPPPTPSPHQKLVIFTEHRDTLNYLESRISTLLGEKDAIAIIHGGIGR
                     EARAKEQEAFKHDPRVKILLATDAAGEGINLQRAHLMINYDLPWNPNRIEQRFGRIHR
                     IGQTEVCHLWNLVAEETREGDVYRKLLDKLEQARESLGGQVFDVLGKLEFEGKTLREL
                     LIAAIRYGERDEVKAYLITIVDKALDRDHIRELLDDHALVHDTMDVSRVFSIREAIER
                     AEARRLQPHYIESFFLEAFHHLGGKAKQRESKRYEITHVPAPVRNRDRLIGIREPVMP
                     RYERIVFEKLLTAPQGQPLAAFVCPGHPLLDSVIDLTLERHRDLLKRGAILVDDKDEG
                     TQPRVLFYLEHAIQDASLTRTGERRVVSKRMIYVELDAEGKSRHMQYAPYLDYRPLKE
                     DEPGPEEILSRPECAWITHELEQTAQKHAVANVVPEHLSEVKANKLSLIAKTEAAVKD
                     RLTKEINYWDHRAEELKLQEQAGKPNARLNSGEARKRADSLQGRLHKRLGELKLEAQI
                     SPLPPVVLGGLLIIPKGLLAVMAGKPVLATTQTTDTQAAAAKARAIIMDIERSLGFDP
                     TDREFDKLGYDIESRVPGTGRLRFIEVKGRISGAPTITVTRNEILYSLNKPEDFILAI
                     VEFMGENGHEVHYVRQPFQREPDFGVTSVNYDFKELLGKATQPY"
     gene            complement(23099..23290)
                     /locus_tag="PLG98_06430"
     CDS             complement(23099..23290)
                     /locus_tag="PLG98_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32927.1"
                     /translation="MKTCSNCGQPIQENDMKNAAEILGELEESSSDDFDDQNLCQACK
                     EELGMLSLMGFGARSCKLS"
BASE COUNT         5575 a         6235 c         5538 g         6217 t
ORIGIN      
        1 cggaaccgga aattcccaac ccccgctggc tcaaggtgaa gaacctggcc tgcggactgt
       61 gcggcacgga catccacttc atgttcatgg aaatggaccc caagtgcttt cccgcggcga
      121 ccccgggcat tgcccggaag tacctggggc acgaactgct cggggaagtg atggaaacgg
      181 ggaatgaagt tgaaggattg aaaaaaggcg accgggtttg ccttcgaatc gactggccct
      241 cctgcttcca gatggaaatg gacccgctgt gcccgcagtg cgcccaggga gaatacatgc
      301 tctgccagaa cctgggagca agggaactgc ccctgacgga tgtgggaggc gggttttccc
      361 cccgaatggt gatgcaccgg acgcagccct tcaaggttcc ggacaacctg accaatgacg
      421 aggcccttct gctggaaccc atggcctgcg ccgtgcacgc ggtcctgaag cattcaccga
      481 cggcgggaaa ccgcgtgctg gtcgtcggat gcggcaccat cgggctcctg accatcgcta
      541 cggtgaaggc cctggccaaa gacgccgaag tgaccgcgct ggcgcgttat ccctttcagg
      601 ccgacatggc ccgcgcgctg ggcgcggacg aagtcatcac ggggcgggag gacccctacc
      661 gccgcatcgc caaacaaacc ggagcgaaat atttcgaggg gtatttcggc aaccaaatcc
      721 tcctgggagg gtatgacgtc atctacgatt ccatcggcaa tgacggcagt ctgaacaacg
      781 cgctccgttg ggcgaaaagc cggggcacca tcgtgattgt gggcgtcaac ttcaaaccgg
      841 ggaaaatcga ctattcgccg atgtggcatc aggaactgca tgtcacgggc atcaactgcc
      901 atattcttca ggaagggtct cttccctcct ctttcgatat cgccgcccaa ctgattgcgg
      961 acaagcgtct gcccgtaagc cgactcatca ctcaccgctt tccgatggac cgctggcgcg
     1021 acgcggtgaa aacatttctt tccaaagggg acaggggagc gatcaagatc gtcctcgaac
     1081 atccttccgc ctaggcaccc ggctccagat tcggctcttc cggagcacga gccgaatgta
     1141 gggtattcca ctcattattt ttcttgcgag cttttcattg acatgcacca gaaactcctt
     1201 tatatccaca tgccattgcg caaattcaac tctcgcggcg gtatgcgtgg aagacaaaga
     1261 aagcaaccag ataccgactg accgttcctg gcaagaacgt tttttcatat acaccggcat
     1321 tctcgcatct tttgtcgcgc tgtatctggt caaaaaccac aactacgtcc tttttcacag
     1381 cctggtcgaa gtcttcagca tcatcatcgc cgccgccatc ttcgccctgg cgtggaacgc
     1441 ccgccgatac ttcgacaacg gctacattct ttttatcggc atttcctttc tgttcgtcgg
     1501 cttcatcgat ctcatccata ccctggccta caagggaatg ggcgtttatc ccggctacaa
     1561 tagcgatctg ccgacgcagc tctggatcgc cgcgcgctgg cttcaggcgc tggctttttt
     1621 tgccgcgaca ttttttctgg atcgaaaact gaaccggccc ctggtggttc tggccggagg
     1681 ggcggttttg tcggcccttc tcttgctttc catcttttac tggaacatct ttccctcctg
     1741 ttttgtggaa ggaaccggac tcaccccgtt caagattgcc agcgaatacg gcatttccct
     1801 tattttgctc atttccatcg tcccgctgac gaaaaagaaa gacaagctcc atccccgggt
     1861 gcggcagctc atcatcgcct cgattctctc tacggttgcc tcggaaatgt ccttcacgct
     1921 ttatacggat gcctatggct tcttcaacgc cctgggccac tttttcaagg tgatcgcttt
     1981 catattcatt tacaaggccg tcgtctcgat tggcctgaaa cagccctatg atttgttgtt
     2041 ccgggacctg aagattcggc aggacgaatt gcgccgaagc gaggagcgct atcactccct
     2101 tttcaagaac atgctcgaag gcatggccta ctgcaaaatg gagtttgacg cggacggccg
     2161 cccggtggat ttcatctatc ttgcggtcaa cgacgcgttt gaaaaactaa ccgggctgaa
     2221 aaacgtcgaa ggaaagcgga tcaccgaaat catgccctcc atcagtaagg agaatcccga
     2281 tttcctcgaa ttgtacggtc gcgttgcgac gaccggccaa cctgaaaagc gcgagcttct
     2341 actcaagccg ctggatttgt ggttgtacct ttcggtttac agcatagaaa agggatcgtt
     2401 tgtctctgtg tttgaaaaca tcaccgaacg caaacaggtg gagaaagact accggagcat
     2461 tttcgaaaat gtccaggaag gcgttttccg ttccacgccg gacggaaaaa ttatcctggc
     2521 caacgaagcc atggcaaaga tcttcggcta cgattccccc gcggaaatca tggcagacat
     2581 caccgatgtg gcagctcagc tttacgtcca cccggaagag cgagcgagcg ttaagaaaat
     2641 catcgccgag caaaacgcca ttcaaaacca cgaggtacag tttcaccgga aggacggcgg
     2701 cgccttttgg ggctctgcaa ccatgaaggc cattcgcaac ggcaaggacc aaatccttta
     2761 ttacgaaggg atcatcgagg acatcaccga acggaaactg agcatcgagc gcctgcgaaa
     2821 aaccctgggg ggcgcggttc aggccatcgc gtcgctggtc gaaacaagag acccttacac
     2881 ggccggccat cagagacgcg tggcggatct ggcgcgggcc atcgcaacgg aaatgggcct
     2941 ctccggcgac cagatcgacg gcattcgcat cgccggaacc attcacgatc tgggcaaaat
     3001 ctccgttcct tcggaaatcc tcaccatccc gcggaaactg accgctctgg aatacagcct
     3061 gatcaaaaat catccgcagt ccggatacga cattctcaag gacatcgaat tcccctggcc
     3121 gatcgccaga atgattgtgg agcaccatga acggatcaac gggtccggct atccgaacgg
     3181 actgcggggc gaggatctgc tgctcgaatc caaaatcctg gccgtggccg acgtggtgga
     3241 agccatggcc acccatcgtc cgtatcgccc cgcgctgggc attgacgcgg cgctggcgga
     3301 gattaccgca aaccggggaa ttctctacga cccggaagtg gtggacgcgt gcctgcggct
     3361 tttccgcgac aaggggtatc agatcagcca ttgatatctg ccggcctgcg cgccgtggcg
     3421 aaaacgcggc attcccagga cggattctat tggacatatt tttagacaaa ttctacttga
     3481 taaatcacaa taatgatgta tttataatgc gttagaaagt aaaaaagctg gacattttta
     3541 tggacattac gggagagaaa atgacatctg gtgagttcag gaagcttgtg gaaaccgccc
     3601 ggttcggtcc gtttatcttt caaatcggcg tggattacca tgaggcggga atgctcctgc
     3661 agcgggtttc cgacgcgcag tcgcggttct tgagtgctcc gctttcccaa gtggccaacc
     3721 gtctggagca ggaagttttg gtcagcagca tcttcagcac gaacaccatc gagggaggca
     3781 cgcttaccga agaagaaacg aaagatgctc tagatatcaa cccaactcag gcacaggccg
     3841 aagaacagcg acgcgccgtg aacatcaagg cggcttatga ccttgcccaa aatgcagctc
     3901 aagaccttcg ctggcgcttg tcggtcgatt tcataaaaca ccttcatgcc gccataacgg
     3961 atggcatttc ccatgcgtac agtcggcccg gtttgatacg gaacaatccc aaaaatgtcg
     4021 tcacccgcgt cggcgacaca gcacatggcg gcacctataa accaccgcaa tatgaaggcg
     4081 atattgaact tttgctccat cacctggccg cctggcacaa tgaaatggcg gacgccgaga
     4141 tcccggcgct gattcgcgca ccgttgacgc attactactt tgaattgatt catccgttct
     4201 gggacggcaa tgggcgcgtc ggccgcgtgc ttgaagcgac agtgcttcat ggagccggat
     4261 tccggtatgc acccttcgcg atcgcccgtt actacctgga acggatcgac cgctacttta
     4321 cgctcttcaa tctctgccgc aaacaggcgg atcagaaaaa ggcatacacc aacacgcctt
     4381 tcgccctgtt tcatctcgaa ggcatgctga tcggcctcaa tcgccttcac gaccgggtga
     4441 atgccatcgt aaaacagctt ttatttgaaa cacgcattcg acatctgcgg gacacaaaag
     4501 agatcaatct gcgtcaatac gtcatcctta cgcagatgat ggaacgtgac aggcccttaa
     4561 agttcgatga actgcgccgc gcgccctggt atctggttct ctatgccaag ctgggcgaaa
     4621 agaccaaaca gcgggattta agcgggcttc gcgatcaggc gcttctggtt gtggatgaga
     4681 agggactcgt ttggccgggg ttgtgagtgc aaaataagaa tcgtcaatcc tcgacaataa
     4741 gcaccgctcc acgtatgcta cgtgtcgttg atggcaagag aggaaagaaa cgggcgggaa
     4801 taagtgccag ggtaactcct catgctctcc gttgattaaa gttgtatctg cagattaaat
     4861 gttattttat ttatattttc gtagagcttc attctgaaca aacagatcac cggcaatttc
     4921 ttcaatcaca tgcttcagct ccagttcttc aagccagggc gcaggaattg ccgccttccc
     4981 ctgcatggcc cccagaatat tgccggtgat cgaaccggtg ctgtccgaat cgccggtatg
     5041 attgacggcc aggcgaaccc ctttggcaaa atcgccttcc gcggccaggg cacagtaaag
     5101 tgaaatcgcc agcgcctctt cgccaaccca accaccgccc attttttcga tggtttccgg
     5161 agcgggcttt gccgtttttt tcttccacag attcaacgcc agcttaatcg cattcagtgt
     5221 ctcctcgtat ccctcggcca tcgcaaggat cgggagcgtt tcatgaatag attcctcaat
     5281 cgtaagtccg gaaataatgc cgtagatgat tgtcgccaga catcccgcag gcaggtaacc
     5341 ggacgggtgc ccatgcgtaa tcgcggcagc ccggcaaccc agatcgaata tttctttcca
     5401 tacttcgccg tgattcccct cggattgcat agctttagcc gcaaacagac cgaccggcgc
     5461 ggcgcgcatc acaccgccgc atcccttgct gttattgatc ggtttttccg gtgtccccat
     5521 cttcgcgctt tgcagagcgg aaaggcaggt gttgcccggc gcccggcgat ggtgcagatc
     5581 ggcaaccgtc agcagccatc cattctgagc gagatcgaaa tcctcgtggg ctgatcttgc
     5641 gccctgcgtt gaaagccatc gcagataaga gcgatgaacc atctcaggat attccgccgg
     5701 aacggctccc tgctttttac gacaccaggc ccgcaaaagc ccttccgccg tgaaaagcgt
     5761 catttgcgtg tcatcggtga tcgcgccttt ccggccgtag cagggcgcgt aattctcaat
     5821 gccgttttcc ccgtattgcc tgcggatggc gccgatggaa tcaaactcga ccgccgcacc
     5881 cagcgcatcg cccacagcgc cgcccagcag acaacccgcg aaatgattaa cattcagccg
     5941 cctgttcacc ggctggcagg agtaaacata tttttcctga tcttgtgttt cgattgttcc
     6001 atgacgggca tcgcgcaccg cctgaattgc cttttcatgc ggcatgccca gttccaccag
     6061 caattgagcc gccaccgtac cggttcgtcc cagaccgccc cggcagtgga tcagaatctt
     6121 tccgccgtcc agcagaattt gtcgcagttt cggcccggca cttttccaaa gtaccataaa
     6181 ccttccatcg ggcctgtgac catcaacaat cggcagatga aaatattcca tcggacagga
     6241 acgaacttgc gccacataat ccggaacgcg aagaagttca tattcatgct cttccatcag
     6301 gttcaccaga gcccgcgcgc cccaggcctg aatcgctgtc aaatcaatat ccagatcacg
     6361 attccacagg ccggactggg cactgttgat tttcttgccg gggcataatg tcataccgat
     6421 gatgccggcg ccgttgggca ccttcacttc atcaatccgc aggggatggg aaatgcttgt
     6481 tttaaccatg ttcagatttc ttgtgtgcat taattaccaa taatcatgtg cttcatataa
     6541 tcttggattg ctctataggt ttgtcatttc gggttgcggg ttcagcaaat ccaaagcaat
     6601 gccggaaaat gtgtgaatac aaattgactc tttattggaa aaaaacacga gttttattcc
     6661 ttctcaaacc cttgactggt gaatttcagg gtgcggctgt tttctgtgac ggtgcggaca
     6721 acctgatccg gagctcctgt cggtatttcg gcctcgattt ccagcgtgac agtgactttg
     6781 gcattgacga gtccggcaag atgagaaatg acctcctcgg cgattcttcc ggcatcacgt
     6841 ccgacacgcg tggcatccag cgtaactgtt ccatggaacc gttttggctg aactgccttg
     6901 ggggttgtat cgatttcctc cttcttcccc tcttcctttt ttgttcctgt cgcttcctgg
     6961 ccgccttccc ttggcttttc gccggctgct tttgcctttt ccgcatctat ttgcaaaact
     7021 gcgatgtctg gctttacaag aagcccggat gtatcgctgt ccgtcagcga tatttgctgg
     7081 ccgcagcgca atccgcgata acgtccggct gcttcgtcaa agctgtcggc aaaagcaaag
     7141 gaatcctgca tccaggtcaa taaagccacg ccgtcgcgaa tggcgctgat caacacggat
     7201 gaatctttca gtcgtggaag atacgtatag ctggcaaagt catcggccaa ctgcttgacg
     7261 gaaacatgat tgccgcgcca gagggggatt ttgtctaaat ccatgcgcaa taatgatgcg
     7321 gcataagcgg gattcaataa catatcattt ttcagctttt tgccggcgcg aacagctaac
     7381 gcatcctgac cggtcacacg aatagcctgc cagtttattt catcctgggg cgttttctgc
     7441 tccggcgtca gcagccactg ataggtttcc ggaagccgcg ctgccaccac tgtatcggcg
     7501 gcatttttct gattttccgc ctgtttgacc tgctgaggat caaggttcaa ttgcaccttc
     7561 tccgcaacga tggattccca cgccaggtat ttgcaaacgg cctcttcaag atcctgcaag
     7621 cgtgttttgt cggctgctaa aaataccagc gcattcctgt aatgacgcgg cgatgtacct
     7681 cgcaactcca gaatggattg cgccgctttc tgagcctcgc ttacgtcccc tttggtgtag
     7741 ggataatcga tccccaaaac aaccagacgc gcatccagat catccgggac gtcctggcct
     7801 gttttcggca atggatggac acggctaaac tcgcctttct tttccaaatc tttgcgcaat
     7861 ctgctttcta tttcctgagc aactttatcc gggtctcttt taatctgttc ggaacgatct
     7921 tcagccagtt ttaaaaccgt cggctgcatc gaataccagt agcgcggacc gtcttggtaa
     7981 agatatgtag ccgaacccgc cagacggcgc agcgcatcgc cgaaaacaga aggagattct
     8041 ccgggcatga cacaaccaag tttcacgcgc cggtcttcaa tgccccgatg agctgctttt
     8101 gttaagggcg ctgatcccag gtaaatcgtt cgcgctacgc ggcgacatgc ggaatattta
     8161 ccgaatgttg cgctgagttc gccatccaac cttagcggca gagaacttgc gccatccacg
     8221 tccttttcaa tgatcggcac ccagttatcc gtcagatagc gggtcaattc cgattgaacg
     8281 cgcggatcgt ccatagcgat attggccggc agaatcagcg gattgcggtc tcccttttcc
     8341 cagaggctgt gaatcacggc ggccataagc cgcaaaacgc cccgcgtccg ctggaacttc
     8401 acaagcgttg accaatcggt atagagacgg tcgaatactt ccgggtgaat cggataagcg
     8461 gctttcattc ttttttcata gtctgcctca cggcattccg gcggaaattc agactgctgc
     8521 gccttgtaga agtctgcaaa tgcgcgggcc acaacatcgc gatctgtaaa ctgggctgaa
     8581 ttggtcatgg gctggaaaag acgccggcga acgatttcaa acccctcttc agcgctggcc
     8641 ggacgccagg aggattcgat gcgccccacc acattgcgta gccgctccag cgcatcgcgg
     8701 cctcgttgcc cgcccacttc cacgtcatcc gcctgtgtgt gcggcgaagg accgccatcc
     8761 gatgccggaa ggctgatcaa cagaagacag ttttttgaga gcttggctga ttccgtgagc
     8821 acctgagcaa aggtaaactg tgtttcaaaa cttcccgcgg gcagatcgct ttgttcgtga
     8881 agctgccggg cgtaggccac ccattcatcg attaaaatca ggcagggtcc gtattcctta
     8941 aacagttcgc gcaagacatc gccggggctg gtcgcttttt catcatcggc cgccacacgg
     9001 gcgaaggctt tctttgcttc tttgatgcca cctgccgcgt gccctaactg ccaggccaat
     9061 tctccccaaa gcgttttaat caccgtgccg tcggatttct tcaccgggtt gccgggtgat
     9121 attttgttgc ccaccagcac aacacggcgg acggagggaa gtttcgaggc atcggctgtc
     9181 tgcataaccg catcgatgcc cgcaaggtct gtgggggcaa ttccggaaaa tagatggtac
     9241 agcgccatca tggaatgtgt tttgccgccg ccgaaattgg tctgaagctg aataacgggg
     9301 tcgccgccct tgccggacag tcgccgcacg gagttcacca gcatggcctt gaggctttcc
     9361 gtcagatagg tgcgacgaaa gaactctgac ggatcgcgat actcatccgt gccttcgttc
     9421 atgtgcacct gccacaaatc cgcggcaaat tcggcctgtg aataccggcc gctggccacg
     9481 tctttgtgcg gcgtcaccac ttcccgccaa ggcttgaggc tggtcgaaac cgcgctttca
     9541 atggccgctc ccgcactcct tcgtttctcc gtgcgcacct gctcgtcata aatcgtgcgc
     9601 cgcagttcca ttttgatttt ccggacttca tcggcctgcg gcgcggatac ggcggaaagc
     9661 aatcgctcgg cggaatccag cgcccggtct gcatcatctc cggaaaaagg tttttggtga
     9721 gcccaatcat tacgaacatc ccgcaattca ctgacaatac ttcgttcatt acgtcccagg
     9781 gtcttgcgaa agacagtatc ccacttgctc cagatgacgg aaaggatggc ggcgatatcc
     9841 cactgcggct tgtctttggt gacgaaaagc tttgcctgga tatcactcac ggacgttctg
     9901 gcttcctcaa tccattgctg ggcatactga gctttcagtt ctcgctccac aaatgggacc
     9961 agaccatctt tgagaagctc cattgctttg ccgacacgtt catgattggt gatggccatg
    10021 atctattcct ttagtcattc tcaaataatt cagattgcga tttttgtgat tcttctatct
    10081 cgtggctgtc tgatgccagt cggctgattt ccggccagct ctggaccaga gcgttatagg
    10141 ataacgcctc ggctgcccgc tttttgcgtt cacacagggt ataaagccgg taacaaagtt
    10201 cgcgggcggt ttcagccttt gatcccaact tggcgactat ttttgccgcc tcggcttcac
    10261 cgccggattc aagaactcgg ataaggtgat gcaccatttc ccatgtggtg agccgcttgt
    10321 ctttttccgg gtcccagtcg gcgggcagtt cgcagggttt cattattcta accttaccag
    10381 attttgaatt caaaaagcct gcatcaacaa gcccttccac actggtgttt tttgccttgg
    10441 agagtatttc tgccacgcca tattcgccct cattaaatcc gaactgctcg aaccacgcca
    10501 gtgcccaacg gctgtcggcg tcgaaatcac cttcctgttc cgtcaacgtc tcatcgagaa
    10561 tttggttgat aagagaaagg gcgtctcgca cggtcagcga cttgccttcc gcatccagaa
    10621 cttttgaata acgggtgaaa accgccatgc ccggaccgat ggcagcctgc gccaggtcca
    10681 ccggcgcgat gttgccccgc tgcagatgaa ctagtgcctc gggtaattcg gctttgagcg
    10741 ccgaaataag atcacggcgt gtgatggtct gaacattatc attgcatttt cgacaaacaa
    10801 gaataatgct agatgcaaga gcgtttgttc ccaatccaac agaacgtgct tcacgctctg
    10861 tgcgcatagg ccatgtacca ctaatagcaa aacctgctct gattaccgct tcgagaaata
    10921 tttcccaggc agtgctggcg gtaccataag caccattact ctcggactgc ttaaaagcat
    10981 agtagattgt caccggcaaa actggatgcg ccatctcagc aattcggttc attgcctgag
    11041 tcatgccttt gaggaaaaac acctctgctt tctctttgct tccatggcga tagggcgtag
    11101 caaccaactc ctcggccttg ggcactgcca gagtggcaaa gagatcaggg caaatcggct
    11161 ttagcgagcg gcgaagccag acataaaaga aatctgatag atcagcatag ccaatgttgt
    11221 catagtaggg cggatcggta gagatgcact tatctttact tattgtctgt gtagctgcat
    11281 ctgcttgaat agcagagcca aattgttttg gtgcgagact cactgttgct tctactgaag
    11341 atttgattgt agaaaaccat gatcccacct ttgagaatgg cgatagctcg acaaaatccc
    11401 aagtcatcgg aatagcctga cgagcaaaga gcgctctaac gttctgatta gtgctgttcc
    11461 agctacacaa tgaattggag agatctgacc accgtgaaat agataaacca agatatacca
    11521 ttaccgcgtc ggcgtaccta attacaccat ttccagcatc aagagtcttt ccgtcaccgg
    11581 gaagaccagc ggcaagtgca tcgtgtatta gcaattctcg cgattcctgc accatatcgg
    11641 agaaggtcgt cagcgccaca agctgccgtg gagtaaaaat gtcgccataa tgcattagac
    11701 catacaactg cgtgcgaata tctcttgggt tatgattgat ctcttgctct ggtatccatg
    11761 aaggcattgc atcttgtgca gctttttccg atacgggatc gggtgcaaga taaacacggc
    11821 cattcggtac ttctgcaact atggccatca tgcgcttgcc aaaagtgcca gctatcccct
    11881 gctcacgaat atacgtaacc ggtattggtg atccagacat caaacagatg aagtgaccac
    11941 cacgacctac cttagtccca ttttttgtag cctgcgcatc tttcggctta cccactttca
    12001 cagtaaaccg ataaccatca ttttcaatca ccggttctat atacgcttct ttccctgctt
    12061 tggttgatag catgaaggtg gacacaagcg ggacatccac atgagcaaaa gctggatttg
    12121 ggcttttcac tgttcttgcc caaagccaag cgatcacttt cagcttctga cccaaatatt
    12181 tttttaagtc cggtctttcc tgaagcattt ctttggtgac ttcaatttta ggatataaat
    12241 ggctaatcct cttttcggcg ttatcactca tccattttcc atagtagcgc acatcctcgg
    12301 ctaggccctg agcgccggac catgtgcgca tggctttctc ttccggcgtt ttgctttgcc
    12361 attccggatt caccggcggt ttcccagcaa acattggtgg aatctctatc atagctttgc
    12421 atattaacac cgccacagga ttaaggtcgc ttgcatacgc ttccaatccg aggcgctggg
    12481 cctccagcgg caacgccccg ccgccggcaa aaggatcatg aaaagcgggc agtttgtagc
    12541 ggtcaaaaag ttctttggcc ttcggatggt cagcattttc agcgcaggta cgccgccagc
    12601 tcgtccagat ttcatctctg gcctgctgca ttaccttttc attggtggtg ttttcccact
    12661 tgaccagtgc ttcgataatt ttgaagagcc gctttcgctc tttatcctgc gctttttcag
    12721 tggggaaaag atccggatgc gccgacggat catcaaccat ctgggcaaaa atcacggccc
    12781 gtgccgccgc cagtggccgc ctagcccacc atatatgaat agatctcgga tgtctgcgtg
    12841 tgaatggttg tttttctaag gccgaagcca cgttgattgt ctccagcggc agcgccactt
    12901 caataagttt tttcttatag gtcataatat tattggtctt ttttcggata tccgagggtt
    12961 ttccaccttg tattttttcc gaagtttcgt tcttaatgtt ctgctcaacg ctaggtccgg
    13021 ataatttatc atgcacttca gacgccgtat gagatgtacc aaataaatcc actgattcga
    13081 cacaaacctt aggttctgca aatgcttttg gcttcatttt cgcatatttg tcagcgacaa
    13141 ttccgaccgg ttcaccaaaa aaccggaagt cgggaatatc aagcggcaac acttgtttta
    13201 gtgattcgct attcacacct ttcaattctt cgcgaagctc catcaacagc cgtcctaaaa
    13261 catttccgcc aacaagtgtt ttatcatcaa ccgttttggc accccaaaaa tcgtcctttc
    13321 gggattcctc aacaatggca cgatcacctg tagcccgcag gagtttgcca aattcatccc
    13381 agttctgggc aagctttaca cgcaagcacc agcgcatgat cttaattcta acgacatccc
    13441 aatccggtcg cgaatcattc cgatggggct tactcttcat ctttgccgtc atcggacttg
    13501 cctgctgaac aatcagttgc tgtatttccg gacggtgagg aaaccggcag gcctgataca
    13561 atgcttcaga tgtaggaata tacgttttat tcacctttag agaaaatcct gctgccatgt
    13621 tcgaaagacc accgaataat tctttagtct ttcgaaagac aacacagtct gaacgcatat
    13681 atgttctttt ttctaagtct tttctcattt atcaccaaac cacatcaaaa tcgatagcgc
    13741 gtccataagt tttgcgcggc acaagcggat accacttact taaagggctt gcgcgtggtg
    13801 attccggatc gccccaccag agcgctagcg ggcaattatt gggacagttt ctataagtta
    13861 caatcagcga tccaaagcct aaaccaaatg cactgaatcc aagaggccgc atggcattcg
    13921 atgggttctt gcatagcgct ctgatcttca tgccggcaag aagaaattct tgctccagaa
    13981 gttgacggcc ttcctcagat gaaaaacaat tattcttaag gcttccgcca gtcttgcgaa
    14041 attcaaacgg aaactttttc tcttgatcga tatatgcaat taggttggca tcagccgtta
    14101 ttgtcgccgg ccatagtact tcggatgaat tacgataact tttacggttc tctaacgtca
    14161 ttgaccgcca tagatgaaaa tctatcttct tccctgctct gattgtttcg tctttgagct
    14221 tctccatcat ttgccactca ccaagtgaat gaacgataaa tacaaaaata tgaacgatgg
    14281 ctttcgctgg tgccgcttgt tgaatccata aacttaaatc acttccaata cggtttccac
    14341 taaacagaat atcatccaag tatataaagg caccatcttc ggcaccgcag ctattgatat
    14401 caatgccata ctgttttctc aaacatacat caaatagcgc gagaagatcc tcctgactat
    14461 gaccgtcctt ttggattctc aaaaaattta ctcttttcca aaatgttcga ggatttttac
    14521 cagcaatgtt ttcatttttt atgcgtttat taaaaatatc aatgactaaa tctttcgcaa
    14581 agtacaattg cccgaaaacg tggtcaactt cctttagtag tggcaattgc acatcccgtg
    14641 caaattgcga aatccaccgt tccacatgct cgggagttgg tcttggcatt tcaccctcgc
    14701 ggtaatccgc aatagtggtc gaaattgatt tcagcaggtc acttcgttca gacatgcttg
    14761 ttacatcctt aatctagaat ctatgtgaat ttctgagcca attacttgtt gaatgcatcc
    14821 ctgtggcagc gccacttcga taaggttttg ggatatttca taggttaaat ttttccattc
    14881 ctttgaatcc agcgtctagc gtatcataat ccaatattcc taagaacgat ccgtcaggca
    14941 taacgctcca agattgttct tcattattgt gatgcgagaa tccggttacg tcatgcccac
    15001 cattccgctt cattgtttcg gtctttgacc ggtgaagcat ttcagttatc aaatacatga
    15061 ctccttgctc aataccaatg acaaaaattc cactagattg gtgccatcgg ctaaatacac
    15121 cgttgatcca gctcaaggga tagcttagtt gattgtcgtg gccaggtagt gatttaaatc
    15181 ccataacagc atatacaaat ctttgtccat tgacttcaac agttggcaat tcaatcatct
    15241 gaatagtctt tttcttgcct tcacataacg cgcgacctgc agataccaag cccatcccca
    15301 ctaaccacgt accataattg caccgacgga cgagatccct tggtaactcc acaggacgaa
    15361 tgttttcatc cttccgtcct ggaggatcaa catatgcgat caacgatgga tcacgattgt
    15421 tatcatgttc ctcacggaaa taagttgcaa taccataact tttcttcggt gtaggaacaa
    15481 tgacggagga ccaagccgca agttgtgtca atgcctgccg taaatctgct ttcgccgaag
    15541 gattgtcttt ggtgggatta acgaagctcc ctttcgcttc catcaaagca acatcaccgt
    15601 ttctcttctc aaaaataaaa tccggttcgt tttgcggacg acccgacgcg atttttgcgg
    15661 acagatactg aacagtgcgt aactgttcag agtgcgtaat gttcgcgctt cgcgcagccc
    15721 tttcccatac tgttgcacac ctgtcccaga agacgtatcc ccatttgatc atagtcagat
    15781 aagcaatggc ctctccgaca cgaccagcaa ggcccgccat agcgaagtct tcaagtgcga
    15841 agacactgtt gtcggtctta cgaaggtttc ctgtgttcca aataaatgta tcttcatgct
    15901 ccgcggcaaa taaaaatgca cgacgatcgg caaaagcatt tgtgaatgaa ctggcaagtt
    15961 cggttttctt tgctgcatga aagaattccc atgcggtaaa gccgaggtgt tgggtaaaat
    16021 taccgaatga gcagttagca gtttgccatt tataggaacc tgcgtgtagg cgaatttgct
    16081 tatgtttcgt catcacacta tttttattaa tctctatcat gggaagagaa ctcacacctt
    16141 gcggtggagt tacaacgatt gggagtgctg gaataaaaag ctgtgccacg aattatgtcc
    16201 ttttcaataa gtttctattt taatttcact ctgatctcta tgtctcagat tccagatccg
    16261 atgcctcttt aatatcggtt actcgtttga tctcctgcca taaacgctgc tcacgccatc
    16321 tgagaaatgc ttcatagtca tctttaaaaa gaggtgaatc agcaccagtt ggcaaccagt
    16381 gcgatttcag cacatcatca aacgggaagc ccggtgtttt gcgaatgtct tcaagatata
    16441 atgacggagc gcgattatta ataatctggt ttgtcgttcg gtctatcaat gtacgattca
    16501 aaatgcagtc acgatttttc gcggttttta ttcctttcac tttttccaga taatccgccg
    16561 ggaaaatatg atggtcatcg atgccttcgt cattcattaa tttgctggta atgatgccct
    16621 gtgtgtggaa atcctttgtt ccattggtta acatgaggca aatcgatcca cgatagatcg
    16681 cgcgctgtct gggcgtaata tctctcaacg atttcggatc aaattttaaa gaatcaaccg
    16741 attccggcaa agcacctgct cctaaccagt caacaagttc cagaacatcc ttggctgatt
    16801 tactgttcgg cgcgctttca taagcctggc caaacaccgc acaccaaaac cagcgtttta
    16861 atttctcacg ttgagcgccg gcaatagccg ttttgggtat gccgactttc gccagaacag
    16921 ccgcgagggg tgcgatcatg gtttgataag gcagccattt tggttggata actttgcaat
    16981 tatctctaag aatttcgagg cctgtcttga gtcctacaat gactttatcc caccattcgg
    17041 taatgtcggt tgctttaaga tttaaaacgt cgctgcgctt acaagatggc gccttgcggc
    17101 acgccagtga tattgcctgc aggacgtaat aaggatcaac ttcaaaatca tctataattg
    17161 cattttcgcc acaggctttt tcccagagat tacgtaaatt gatattctga ggccagaaac
    17221 gagccgtcag taattcaaat acactgagct taacacccgt tcggttaagt gtttcaaata
    17281 ttgtacaaag cgcatcaggt tctgtttcat cagacagggt gactaccgga aaatgatagt
    17341 cgtcaatctt catgatccat tgatcattta ttttagttag agcatccagc atttttgtgc
    17401 gttcttcagg cggcatgggg ttggtggcct tcagcaacca tttaaggaaa ccgcctgacc
    17461 cgtttttaag cacactgagg ggcaagataa gttgttgcgc ctgaatatca aaatattcat
    17521 gctcttttac ccactttgtt gtggcacgtg cgtggaaaat ggcttcctca aaatcattcc
    17581 catcgagtag ttttctcaaa tccaaataat aacagtgctc gccgacgcca tagaaagcct
    17641 gataaagtga tgtgaggcgt tgttgtccat caagaaccag gaaggtatgc ttcttgccgt
    17701 ttagagttgg cgccccttca aattcccgtg ctgcaaaaac ccttttctta tctcttacgc
    17761 ggaggagact gcccgcgggg taattattgg caatcgaaat gatgagttct tgcgtggcat
    17821 agggttccca aacaaaatcc ctctgaaaat cgggcaatac tgtcgttagt gaatgaattt
    17881 ctgccagtag ttctttaagt ggtttgggat tggtgtcttc gaaaagactc atgataactt
    17941 ccttctttct taactgttaa tgatgaggct tctgtttgcc gttgttttgt tgatggcagt
    18001 ccacagtaaa gcatattcaa taatcttggt tatttttttg ctttgctttt tttaacgggc
    18061 tttttaggtg ccgccagagt tgctgctttt tgtttttctt catacagagc aacaaagttc
    18121 attggctgta aattcaacgg gacaatgctt gcgcgccggg tcaagtcggc gatggattcc
    18181 cgcacgtagg ggaaaataat ggatgcgcaa ttgacatgag caatgcgctc caatagctct
    18241 tttgcgggta atttttcaaa cacaaatgct ccctcccacg ccacagaaaa tcgaaaaggc
    18301 tgcttttccg aatccgaata gaaccgcacg acaacgccaa gaatattgtc aactgactta
    18361 tgttcgatct caatgctctg ctgaaactct accatctcat ctttaaccca tttatattgc
    18421 cggttaaggg caaagtgcgc ttcaataagc ctgataacat caattctgaa tttaagttcc
    18481 ataagccatt tcctcttcgt cggacgtata tctcgcttct ctgacctgat agccgatctt
    18541 ttcctgcaaa tccttgatat aggtaatttt tgattttgat atgcaatgag cgtcattttt
    18601 ccggataacg ttcaaggtct gcggataggc atccagaatg cgcagcagat tgtccattcc
    18661 tttactctgc gatatctgtc cagtttcata ccgtgcaaca cttttcgcac cgccaccgat
    18721 aatgttagcc agttgttcct gtttaaggcc cagcttcaat cggatcgatt tgatttgcgc
    18781 gccggtgaga agaccatcca cctcccgctg aaaatccttc aagattttcc cggatgtttt
    18841 caatgtttca ctatctacaa tagcttcacc gcattcagga cattcgtagg taacgtaatc
    18901 aggtatttct tttgtttcgt ccttgtaatt gaatacttgt gttttaatgg tctttttcag
    18961 cgcaccagcg ccgcaaatgg gacatttctg cgatttcatt ttaatcatcg ctcccttcat
    19021 cttttttcat ctgaatcaaa actgcttttt taggcacatc cagtgaaaat tgcaatttga
    19081 tgtaaagttt tttctcattg tctgtaatct tgtatacgtc ttgccaaacc ttgtgattag
    19141 cataagaggt catggactta taaaaatgct cggaacaaag ccggtcaatg acgccctcaa
    19201 tgtcttcgat agtcatatac ccttcggcta ctgccccttt gtgggcaatc tgggtaataa
    19261 cccgggtact ttcattgcgg aaaagaacct taaaatcact caatacgtaa tggggtttac
    19321 gcttttctct tacttttggt atcattatga tactattaaa ccgcctgtca acatattttt
    19381 tgaccgaaat taatacggtt gggttgcttt acccaatagt tctttaaaat catagttcac
    19441 gctggtcact ccaaaatccg gttcccgttg aaaaggttgg cgaacataat ggacttcatg
    19501 gccattttcg cccataaatt caacgattgc cagaataaaa tcttcaggtt tattgaggga
    19561 atagaggatt tcattacggg ttacggtaat ggttggtgcc cctgatattc gtcctttgac
    19621 ttcgataaat cgtaacctgc cggttcccgg cacccggctt tcgatgtcgt aaccgagctt
    19681 atcgaattcg cggtcggtcg gatcaaaacc caaactgcgt tcaatgtcca tgatgatggc
    19741 gcgggccttt gcagcggcag cctgggtatc cgttgtctgt gtagttgcca aaacgggctt
    19801 accagccatt acggcaagaa ggcctttggg gatgatcaac aatccgccaa gcaccaccgg
    19861 cggcaagggg gaaatttgcg cttccagctt gagttctccc aatcgtttgt gcaagcgccc
    19921 ctgtaaacta tcggctcgtt ttctcgcctc accggaattg agccgggcgt ttggttttcc
    19981 tgcctgctct tgaagcttaa gttcttctgc acggtggtcc cagtaattga tttccttggt
    20041 cagccgatct tttaccgcag cttccgtttt ggcaatcaat gaaagcttgt ttgctttcac
    20101 ttccgacaaa tgctccggga cgacattcgc cacggcatgt ttttgggctg tctgttccaa
    20161 ttcgtgggtg atccatgcgc attccggacg actgagaatt tcctcaggac cgggttcatc
    20221 ttccttgagc ggccgataat caagataggg ggcatattgc atgtgacgtg acttgccctc
    20281 agcgtcaagc tccacataga tcatgcgttt agaaacaaca cgccgttccc cagttcgagt
    20341 cagactggcg tcctgaatgg catgttcaag ataaaaaaga actcgtggtt gtgtgccttc
    20401 atctttatca tcaaccagta tggcgcccct tttcagcaga tctcgatgtc gctcaagtgt
    20461 cagatcaatg accgaatcca gcaatggatg gcccgggcag acaaacgcag caagaggctg
    20521 cccctgaggg gcggtcagca gtttctcaaa cacaatccgt tcatagcgcg gcatcactgg
    20581 ttcacgaatc ccgatcagcc ggtcgcgatt tctgacgggt gctggaacgt gggtgatttc
    20641 atatcgcttg gattcgcgct gtttggcttt gccccccagg tgatggaagg cttccaggaa
    20701 aaacgattca atataatgcg gttgcagacg ccgggcttcc gctcgttcta tggcttcccg
    20761 aatgctaaat acgcgactaa catccatcgt atcatgaacc agcgcatggt cgtccaataa
    20821 ttcgcgaatg tgatcacgat ctagcgcctt atcgactatc gttatcaggt aggctttaac
    20881 ttcatcccgc tcaccatacc taatggccgc aatgaggagc tcgcgcagtg tcttcccttc
    20941 aaactccagc ttgcccaata catcaaacac ctgcccaccc aatgactcgc gtgcctgttc
    21001 gagcttatcc agtaatttgc gataaacatc accttcacgg gtttcttcag cgacaaggtt
    21061 ccagagatga caaacttccg tttgcccaat acggtgtatg cgtccaaaac gctgttcaat
    21121 gcggttggga ttccacggca ggtcataatt aatcatcaag tgggctcgtt gcaaattgat
    21181 accttcaccg gcggcatcgg ttgccaataa aattttcact ctgggatcat gtttaaaggc
    21241 ttcctgttct ttcgcgcgag cttcccgccc tatgccaccg tgaatgatgg caattgcgtc
    21301 tttttccccc agcaatgtgc tgatgcgact ttccagataa ttcagcgtgt cccgatgttc
    21361 ggtgaagata accagtttct gatgcggaga aggggtggga ggtggtatct ggccggcccc
    21421 gtaaggcggt tttgattcgg caacacatcc gggcgtcatg aatattgcgc ctagcaggct
    21481 ggacaattcg caccattttg cgtcagcacc gctgcggcgg atgatgagcg ctaaggattc
    21541 cagattttta agtgtctcta tttccgcttt taattcgaca atagaccgcg cggcagtcgc
    21601 ctggtcgaga atttcttctt ctgcctcctg gacttcattg tccggcgcat cttccaaatc
    21661 ctccacgtct tccgcatcca atgtccgaag ggccgggtca atgagaggcg ctgcctgacc
    21721 accgcgatgc agtagttcca actctcgcag acggctttcc agcctttccc gacggcgacg
    21781 tagtgattga tatatagcct caggggatga agccaagcgt cgctgcagga tggtcagggc
    21841 aaaccccacg gttccggagc gtttgtcgtt ttccagcgct tccgcacggt tgaattcctc
    21901 gcgaacgtaa tccgtaacgg ctttatacag ttgagcttct gcatcggaga gtttgtaagg
    21961 tacggtatag gcgatccgtt gtggaaataa tggcgtcccg tcaaatttaa gaagattttc
    22021 tttgaccatc cggcgcatca aatcggaaac atcctggacg tgaacaccgt cgcgaaaacg
    22081 tccctcaaaa cgatctccat ccagcagtgc catgaacaac tgaaagtccg tttctttgcc
    22141 gttgtgcggg gttgccgtca tgagcaggaa gtgccgggta accgtcgaaa gaagctgtcc
    22201 gagccggtag cgtttggtgt attttgtgtc cccgccaaaa acggtggctg acatcttgtg
    22261 cgcttcgtcg caaacaacaa ggtcccagcg gcaatcgggc gcttggagct ttaactggac
    22321 gtcttcgtta cgcgccagct tgtcgagcct cgaaatcacc aggtcggttt ccataaacca
    22381 gttgccagtg cgtgcagcct ccagtttatc attggtcagt atttcaaagg gaatgtgaaa
    22441 gcgactccag agttcgtcct gccattgttc ggccagactt ccggggcaga cgatcaggca
    22501 ccgctgcaaa tcgccgcggg cgatcagttc tttgatcagc aatcctgcca tgatggtttt
    22561 gccggcgcca ggatcatcgg ccagcagata gcgcaagggt tgccggggca gcatggattc
    22621 gtaaactgcc gttatctggt gcggcagcgg ttcaacaatg gatgtatgaa tggcaagcaa
    22681 tggatcaaat aaataggcca ggcgtatgcg tcgcgcctcg gatgccagac ggaacattgc
    22741 gccatcgcca tcgaaactcc aaggccgtcc cttctccaca agttccaggc gcggctcgtc
    22801 atgccgataa aggagttcat tggccaattt tccgtcaggt gtcttatagg tgatttccat
    22861 ggcctctgag ccataccact ggacgctgac aactgttatt tggccgtttg gaataatgcc
    22921 ggatattgct gcgttaggtt gaatgtcttc gagtcgtatc attgcggatc cgtcaaaata
    22981 ggtatcgttt ttcttcgaca cagaagtctt gaatatcatc aggattcttt acagacattc
    23041 agcattatcc tgatgcttcg acaagctcaa tatgacaata atggtaaatt gctaattttt
    23101 acgaaagctt acaacttctc gcgccaaacc ccatcaggct gagcatgccc agttcttctt
    23161 tgcaggcttg gcagagattc tgatcgtcaa agtcgtcgga tgacgattcc tcaagctcgc
    23221 ccaaaatctc cgccgcattc ttcatgtcgt tttcctggat gggttgtccg cagttgctgc
    23281 acgttttcat acacctatcc ttttgataat tcaagggttc atcacaaaat ctttattttg
    23341 tgtgaagatt atggccatcc ctctgtaaaa tcaatgtcaa aacgacaacc gtaagtcgca
    23401 ttttttctca atgaggagcg aaattagaca aattagattt cgattgaatg ctttttttca
    23461 cactcgggtt acaagctgta ttattacaat taggctcaat aaagcgcagt ctttattgag
    23521 cctaattgtc aatgagcttt cttggcttcc ctgtagatcc agtga
//
LOCUS       DAOSME010000033        23314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2746_length_23314_cov_24.348424, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000033 DAOSME010000000
VERSION     DAOSME010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23314
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2746_length_23314_cov_24.348424"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLG98_06435"
     CDS             complement(<1..916)
                     /locus_tag="PLG98_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOE32928.1"
                     /translation="MPLIRKYQVNWSIIFVVALIVTGLSVWESRNLKIDTDILESMPH
                     KDPVLADARLIIKHLPVQDKVFINLEQTSPDRNKLIDTAAAVTDQLNKSGLFIRVGIG
                     DDAENFPELIAHVNDNLPALLSADDLEQKIAPLLTPDNIREAMAQNRQALGQLEGIGR
                     GDMMTKDPLGFSSVILKQMSALLPANKALFYQGQLISEDGTNALIVARIKGSGTDTAV
                     AEKIEKLLEDCRRDLAAKGHPYKLTATGAYRVALDNETTAKSDMQMAVTFTTLGIAFL
                     LILAFPRPLIGLLALLPSSVGAIAALFVC"
     gene            complement(1185..1820)
                     /locus_tag="PLG98_06440"
     CDS             complement(1185..1820)
                     /locus_tag="PLG98_06440"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32929.1"
                     /translation="MKKEDYSCCEKMKNPDLDTGIEDMHICRPQISKVEYQDVNRIFE
                     KETLQHEIGFVKLKNGGYLVAMETGRPDVKRKMVRWWFWRHVQEKEHQIVYPGEHLSI
                     RYSSRNKDYFNEPYGDFEKNTVYPVGRAGKKVIRLFIQFVHPRTFGISESNIKRTENG
                     FLLCGFVGVMKGMRKHTKMLHYFKSNGDGIRPISRFGLGNGLPRVLKSIAQ"
     gene            complement(1957..2676)
                     /locus_tag="PLG98_06445"
     CDS             complement(1957..2676)
                     /locus_tag="PLG98_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE32930.1"
                     /translation="MTRSKDYNKNRFAFVIPVYNHAGTVAQVAKDAQKLGYPVVVVDD
                     GSTDDTYNQIKEIKDIQLLQHEQNQGKGAAILTGFTAAAKMADWAIIVDADGQHYPED
                     AKKLIKAIPKNTRPIVVGAREGMAGKHVPWTSSFGKKFSNFWVRASGGPAISDSQSGF
                     RIYPLPEALNLKTKARRFQFEVEILVHAERNGIPVIEAPIRVRYNPEGGRISHFRPFV
                     DFCRNSSTFTRLIFTRIFPPR"
     gene            complement(2692..4413)
                     /locus_tag="PLG98_06450"
     CDS             complement(2692..4413)
                     /locus_tag="PLG98_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOE32931.1"
                     /translation="MNEVLKQSFTAGVERLMARSDLLDSINVFPVADGDTGRNLSLSL
                     MPLRNTDLPQATMIHRLLLSARGNSGNIASQFFSAFCAVENIADLAGRAREGKIKAWQ
                     ALVNPRRGTMLNVFDALADVLSEQFTPDKENIENIIRSMENAVHQTYEQLPKLQEAGV
                     VDAGALGIYIFFEGFFYTLTGLSEDGRPLTEIFHDRLHISSSFQENIESGYCVDFVVK
                     APRYSPDEIKMITSVQEEVIVIPEGEFYKVHLHTQDKEQVRSQITGLGSLVTWEDDDL
                     SCQTADFRHLNAEKTLHIITDAAGSLTTQDAKIHGFTLLNSYLTIGDQCLPETFFHPE
                     DLYRAMKKDIRVSTSQASVYERHQFYQSALERFDKVLYLCVGSVFTGNYQAAVQWKKD
                     HDKEDRFIILDTGAASGRLGTIALSTARYLAESRDVTNTIIFARKAIRLCEEYLFLDK
                     LHYLAAGGRLSKTSAFFGDKFNVKPVISPLPDGAKKVGTAKNQEDQITFALAKLDATI
                     KDDSRALIMLEYSNNRRFVDGTVRKNIKELYPDAEIILQPLSLTSGAHMGPGTWGVAF
                     LPQINIR"
     gene            complement(4415..4831)
                     /locus_tag="PLG98_06455"
     CDS             complement(4415..4831)
                     /locus_tag="PLG98_06455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOE32932.1"
                     /translation="MKEKQNSIDVKIRVPFFDLDPMQIVWHANYLNYFEIARAALFES
                     RGIDLYSFYEKHQLIFPIIKTSTKHVLPLFHRDEIICKATLVEARIKLIVDFEIRKAE
                     DGKIVARGRTEQVTVKVPEREMLFRIPEEISRAFGF"
     gene            complement(4828..6381)
                     /locus_tag="PLG98_06460"
     CDS             complement(4828..6381)
                     /locus_tag="PLG98_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOE32933.1"
                     /translation="MKKNVNRSAVIIDGGKIRFNDIIAVARYGVAVKISKDATFVRKM
                     KKTRDMLMQSMRTGIPIYGVTTGYGKSCGKRMPFDIALKNGVNILRFHGCGTGEPIGV
                     EETRAAMVCRMICLARGYSGVSIALLQQIADFLNHGITPIVPCEGSVGASGDLTPMSY
                     IAATLMGERDVIYRGTVMSAARAMKKAGLKPYTYLPKEPLAMLNGTTTMTGIAALTIE
                     RAYRILSAAIYATALSIHALKGNARHYHPVISEAKPFPGQAFVAAQITRLLKAKISTR
                     HLEDDSLDALQDPYSLRCTPQVAGVLYDGLEWIRTWVEIEANSSNDNPIFDPATGEIL
                     MGGNFYGGHIAFAMDGLKAALASVADMCDRQIMLLVSPNLNRGLPGDLVRKPPHEKGL
                     RHGFKAMSISSSALTAEALKLTMPAASFSRSTESHNQDKVSMGTIAARDAERICTLTE
                     RVVAIHLLAAAQACDIRDNTNARPLLSKTMGKIRSVSAPVIEDRAMDKDIENICTAIR
                     YSDFFKIKP"
     gene            complement(6385..7644)
                     /locus_tag="PLG98_06465"
     CDS             complement(6385..7644)
                     /locus_tag="PLG98_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE32934.1"
                     /translation="MKFKLIYPKWEKLDRQTRFTLPPHGPVVFAATLPDDVDVEFIDE
                     NIKDIDFDEPADFVGISVMLTAQIKRAWDIADRFREKGMKVIFGGIATMLHAEETQQH
                     ADSVFLGEAEGRMEQVFDDFKNGNLQKVYNYMQNFPPIESVGTARRSILDRDMYNYRG
                     VQMVDLVHASRGCRFNCYPCAVRFLGGRQFRPRPFDKVVEEMASIENNRLFIVDNSLA
                     QDKNWETQLFKEMIPLKKKWCCHPIEKDDAVLDLAAQAGAWFVYQTVFDTSDFIRDRI
                     KRYHDFGIGVEGSILLGLDYHTEDYIKKLVDFLLDIELDMAEFTILTPFPHTRTFDDL
                     HNAGRIFSYDWSDYTCGKVVFQPKVLTPGKLQELYYYAWDKFYEEEPANYKMFKLLKK
                     VMDKEKADGTYKPRVRPKVIRDKKGVS"
     gene            complement(7753..8994)
                     /locus_tag="PLG98_06470"
     CDS             complement(7753..8994)
                     /locus_tag="PLG98_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOE32935.1"
                     /translation="MNADRPQDDARRVVVSGIGMVTPLGIGKEEFGRKLFAGHTAIKD
                     IRSFDTGAFSSHLGAEVTEFKPRDFIAVKNMRRMDKISLMTVASARMALEDAGLQITA
                     ENRDRVGIILGTAFGATDITVQFLHTLLQEGPASVNPILVPNTVMNAPAGHTSIELGF
                     RGINTTITHFAVSAENAIAYAAAEIRRGTADFIFAGGSDILSEIAYRSLIGFGVLSPQ
                     DKGNEICRPFDQERNGCIAGEGCGMILLESLQSAKQRGCNYYCEISGAGMGASPTRPN
                     EWPRDTEGIKRTFARAVKNAGITISDLQAIFAAANGDKILDAAEAEAYAEIFDETKRP
                     CITSIKGATGESFSSGGIKACAAALSMANQSLPPTAGLAKPVRALEFVIQEKKEADIH
                     HTALAGISFGGTYCYLIFSKL"
     gene            complement(8984..10231)
                     /locus_tag="PLG98_06475"
     CDS             complement(8984..10231)
                     /locus_tag="PLG98_06475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOE32936.1"
                     /translation="MQKRIVITGMGTLNAIAHNIPDFSPALKNGHCGIREIDIFDTSG
                     FSARTGGQIKNFAARNYIPQNFSMKRMSRADLLSFAATLEALHDAGLYPFPEELQKDT
                     GVSIGGGAGGLLEAESFYANFLKSNGRRARFSRLASLFCASSADRIASAFGLAGPKAT
                     FMTACSGGATAIGYARDLIAGGQASVVIAGGVEPMCRLTYAAFNALKSVAAATCRPFD
                     KNRDGLSLGEAAAIVILEELDSALARGARIHGEILGYGITCDSYHMTAPDEQASGAVR
                     SMDAALRDSGLSPDDIDYINAHGTATPVNDAMETKAIKNVFGKRAYTIPVSSTKSMHA
                     HTLGAAGALEAIVSILALRDGFVPPTINHQERDPQCDLDYITSGARSANLKTALSNSF
                     AFGGNNTTLIFGKYTERSARHER"
     gene            10499..11026
                     /locus_tag="PLG98_06480"
     CDS             10499..11026
                     /locus_tag="PLG98_06480"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOE32937.1"
                     /translation="MAKKNPETKYEEKVFVKERIIAKRLKNSKKEAADQEIKAARKAE
                     RLDEENEVLITILSEIENLPKEDILYNYSEDISMTGTRIQGNCLLPVDTFLKIDLVLK
                     NLKQTVTVFGKVKWSKPAEDVKSYEAGVEFVDTPEESIKKLGDYILSINQYKNLNPVG
                     VPYWIFAKFNKPSSK"
     gene            11299..11574
                     /locus_tag="PLG98_06485"
     CDS             11299..11574
                     /locus_tag="PLG98_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32938.1"
                     /translation="MKKTLRTIAVAAAVVIFTATFGFAEYAATGAADFPYFQLGCLIV
                     GGLILMTLKRKYEKMYVAEMVGAFALYTLLMALFTNPVIDAVKTMVM"
     gene            11805..12299
                     /locus_tag="PLG98_06490"
     CDS             11805..12299
                     /locus_tag="PLG98_06490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE32939.1"
                     /translation="MNFLALDFETANYYQDSACALGLVRVEDNKIIHRTSFLIRPPYR
                     YFVFTHIHGITWGDVEDQPTFDQCWKSIKPFFEGIDFAVAHNAGFDKGVLRACCRRYA
                     ITPPDLDFQCTMKLSRKVFNLYPTNLPAVCRNFNIDLQHHDALSDTLACAKIMIEIIR
                     KTNT"
     gene            12594..13835
                     /locus_tag="PLG98_06495"
     CDS             12594..13835
                     /locus_tag="PLG98_06495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOE32940.1"
                     /translation="MTSKQNIRVVITGVGLTAPNANHLNDFRRALLNQKSGISEMEVR
                     YMGKVAAGNCNFDEYLYQKKKMRRRGTRAGSISIYCAHEALKDSGLSLESLVKDRTGI
                     YLGITEHGNVETENEIYELHQNNLDAGLWSHHHNPRTVANTPAGEVSLNTGITGPAYT
                     IGAACAAGNIGVIHGMQMLQLGEIDLALAGGVSESTGTFGIFAGFKSQGALGSHEDPT
                     KASRPLDINRNGIVVSEGGAVFVLERLEDALQRGAKIYGEIAGYHVNSDAVDFVLPDT
                     QRQIQCINTAMAKANISPRDIDIVNLHATGTVSGDENECEAVRAVFGQSDNTYVNCTK
                     GFIGHAMGAAGALELAGNLPSFTDHKIHSCKYIENVDPKCNVKNLVNGDPVKKDVNFI
                     LSNSFGMLGINSVLIVKKYKQ"
     gene            13856..14113
                     /locus_tag="PLG98_06500"
     CDS             13856..14113
                     /locus_tag="PLG98_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOE32941.1"
                     /translation="MDYQTIRKKIIDIIAEIAIDEDLSNLDDNIRLREQLDMDSMDFL
                     DVVMELKKRYRIEVPTEDYMKLATLKSCIEYLGPKFAVSPA"
     gene            14113..15498
                     /locus_tag="PLG98_06505"
     CDS             14113..15498
                     /locus_tag="PLG98_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE32942.1"
                     /translation="MTQYDTVIIGAGMSGLAAGIRLAMFDQKVCILERHKIPGGLNSY
                     YDRQGRKLDVGLHAMTNFARQGERGRPLTKLLKQLRIPYEQLELSQQNFSRIVFPAHD
                     LAFANDPQLLIQDIGKHFPHQKDPFARLLDVVINFDDVNLSNKPQSAKEKVREIISDE
                     YLLEMLFCPLLIYGSAWENDMDFSQFVIMFKSIYLEGFSRPRNGVRTIISLLIEKYKQ
                     AGGELRLGTGVKKIDVENESVTGVTLDSGESIRTDKIMSSIGLPETMNIVSEKSDRPA
                     VGNMSFAENLLFFDKKPVEAGIKETIIFYNDNDTYDYKKSRDLCDYRSAVICLPNNFG
                     TDDYDEGLIRITFIANFDQWNALDRKTYLQKKKEVCNAALSLVAKVIPQFQAKLLYHD
                     VFTPKTINRFTGHFKGAVYGTTEKVRDGRTGIKNLFICGTDQGFLGIVGAMLSGISMA
                     NLHCLMNGEKA"
     gene            15495..16526
                     /locus_tag="PLG98_06510"
     CDS             15495..16526
                     /locus_tag="PLG98_06510"
                     /inference="COORDINATES: protein motif:HMM:NF006720.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HOE32943.1"
                     /translation="MRFKNAVISSFAYHLPDQVMTSEEIEERLAPLYERLKLPAGRVE
                     LMTGIKERRFWPPGTMPSDLSTQAARNLFEKNIVQPDQIDLLIHASVCRDFLEPSTSS
                     IVHHNLELQTDIMFFDLSNACLGVMSSFVVAANMIDNGFIKHALIVSGENGGPLLFQT
                     IDRLLNDKNITRKSIKKYIANLTIGSAAVAYTVSHKDHVHEGHCLLGGASLVDSSANV
                     LCRGGGDTQSLMMETDSEELMKSGVALAEKTWAETKRELGWTHDDVNCFVGHQVGVAH
                     ETLLKQILKLEHCPTFTTYQFLGNTGASALPVTLAIYDEEKKIPAGSQVGLLGIGSGL
                     NSIMLGVKW"
     gene            16520..17380
                     /locus_tag="PLG98_06515"
     CDS             16520..17380
                     /locus_tag="PLG98_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE32944.1"
                     /translation="MVRPDWLNKLYPFTSHFYQLKDNISLHYLDEGQGDPMLMLHGNP
                     TWSFYYRKLVAAFSISNRVIVPDHIGCGLSDKPQQYAYTLHQHIRNLDKLVSYLELKN
                     VVLVVHDWGGPIGFGLLKRRPELVKKIVILNSAAYTSKVIAFQINMCRIPILAEQVIR
                     RLNGFALAATYMAVAKKMSPEIRKGYLYPYDNYENRIAIAQFVADIPMTPGHRSYQTL
                     KSVELSLAQHRCPVLILWGKRDFCFHDYFLNRWQEIYPHATVKTFEDAGHYVLEDAGD
                     DIIAELGKFV"
     gene            17413..19038
                     /locus_tag="PLG98_06520"
     CDS             17413..19038
                     /locus_tag="PLG98_06520"
                     /inference="COORDINATES: protein motif:HMM:NF006754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HOE32945.1"
                     /translation="MNIADRFEEIAKIHHDKVAVKFPVRTGKKYRYKAITFGELETLA
                     GKYANGLSEIGFRRGCKALLFVRPSLKFPALVFALFKIGVIPVFIDPGMGRKNLLAAI
                     EEVAPEGVIAEPEVHLLRILFNNSFKSVRFKVTTKGPKIGNCFPLSMLKRARAGFQSA
                     QLEPDETAAILFTSGGTGCPKGVVYTHKIYYEQTRLLQELFSLDENEIDFPCFPLFSL
                     FTLCMGMTSCIPDMNPSQPAKADPKQLVQNIKDNKVTFMAGSPAIWEALGSYCIKNNI
                     TLPSVKYLVMFGAPVRNELHKRFSRILPNGTTYTPYGATESLPVANISGQMVLEQTAA
                     LTDEGKGTCVGEPAPGVKVAIAAINDNIIPHIRQARILGLHQIGEIIVKGDMVTAGYY
                     QSPQETALAKIADGASFWHRMGDLGYIDDAGKLWFCGRKSHRVETPSGLLCSVMCEAI
                     FNTHNGVRRSALVGIGEKGRQSAAIVIERNSAGRKLDRKRLEKELLGLGGKNPLTAAI
                     TSVHFCDSFPVDVRHNIKIDRLKLADDVARGRI"
     gene            19035..20030
                     /locus_tag="PLG98_06525"
     CDS             19035..20030
                     /locus_tag="PLG98_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE32946.1"
                     /translation="MNIIVTGAGGFLGRNLANGLLSKGHKVRNFSRRNHLPLQAMGVE
                     TICGDLQNASEVMAAFQGIDAVFHVASRVGIWGDYDEYYRTNVVGTGNVIKACRANGI
                     TKLIYTSTPSVVFDGNDILRADETKPYALKFYNDYSRTKTLAEQKVLQANDETLSTVA
                     LRPHQIIGPDDPHLVPRLVNAAKRGRLKIVGTGKNLVDVTYIQNAVEAHLLAFEKLSP
                     QSSVAGQAYFIGQERPVVLWDFINKILTRYNLSPITKRVPEKVALNMGALFEAWYRFF
                     KINGEPPMTRFVALQFSRSHYFSHEKAKNDFGYVPKISIEQALDLTTERGMNAFK"
     gene            20017..>20264
                     /locus_tag="PLG98_06530"
     CDS             20017..>20264
                     /locus_tag="PLG98_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE32947.1"
                     /translation="MLLNKNDKITQHYDVIIIGGGLGGLTAANKLAKNGRKVLILESH
                     DKLGGFATWFYRKKHIFDVSLHGFPVGMIKTCRKYWNKA"
     assembly_gap    20265..20364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20365..21225
                     /locus_tag="PLG98_06535"
     CDS             <20365..21225
                     /locus_tag="PLG98_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32948.1"
                     /translation="VFSNFMSKGVYTFQGGTDLVIGMMQEELMKNNVDIRLKSNVGKI
                     VVENNVCRGVMLGGEFVSSTSVLSNGNLIGTIFNLTGEDNFKPEFIDKVKKVRLNTSS
                     CQVYMGIRQGEAIEDIGDLIFYSEAKEFETDELLSMNTTSRTFSFYYPRLRPQLENAG
                     YTIVASTNARYEDWAELSKEDYKQKKRELIEATLVSLEKIIPGIRQKIDYVEAATPLT
                     IKRYTHHEGGSSFGTKHEGLDISMKLHHEISGLFHTGSVGIIMSGWLGAANYGVIQSY
                     EVEGYLDKLN"
     gene            21234..>21844
                     /locus_tag="PLG98_06540"
     CDS             21234..>21844
                     /locus_tag="PLG98_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE32949.1"
                     /translation="MFDFSNQRVIVTGGTRGIGAGIAEAFLQAGATVIAVYSANDAAA
                     NEFKNKNEQFSDRLDIQKQDISSSQDVNNLFQYLDEKYPSLEVLVNNSGIRQDAIAAL
                     MTDEQWNKVLEVNLTGTFYMSREIIPRFMSGRFGRIINISSLGGDIGFQGQANYAASK
                     AGQLAIAKSLSKEVARKGITVNCVSPGFIDTELIGDLPEEQRK"
     assembly_gap    21845..21944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22108..22899
                     /locus_tag="PLG98_06545"
     CDS             22108..22899
                     /locus_tag="PLG98_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE32950.1"
                     /translation="MDFLQVSGKTFLVTGVANKKSVAFFVAKGLIENGAQVIFSVQNE
                     ENKDRAQKLFPDSTVLICDVEKTGSINRLGEYFKNHNIKLAGFVHSIAFANYSEGLKP
                     FHETKPEDFLQAANISCFSLVAVANALKNSFENDASIVTISISNTRATAYGYMGPIKA
                     ALDATVAFLAKSLSEMSKARVNAVCAGPLKTSASAGIPGYVNNYLFAEQLTLRKAALK
                     TEEVANTVLFLLSPASGGINATGVVVDAGMSCNYFDQDVVNKAMR"
     gene            22920..>23314
                     /gene="mobB"
                     /locus_tag="PLG98_06550"
     CDS             22920..>23314
                     /gene="mobB"
                     /locus_tag="PLG98_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOE32951.1"
                     /translation="MTVPLISIVGKSNSGKTTLVEKLIPELIKRGYRVATIKHNKHGF
                     DIDHEGKDSWRHKKAGACATVIACPTRVALIEDLERDQTLGEIRDKYIKSADVILAEG
                     YKGNPFPKIEVFRSELKRELLCGKEDNLLA"
BASE COUNT         5965 a         5498 c         5782 g         5869 t
ORIGIN      
        1 aacaaacgaa gagagcggca atcgcgccga cggacgaagg cagcagcgcc agcaggccga
       61 tgagcggacg cggaaacgca aggatgagca aaaaagcgat gcccagggtt gtgaaggtta
      121 cggccatctg catatcgctt ttggctgtgg tttcgttatc cagcgccaca cggtaagcgc
      181 ccgtggcagt cagtttatat ggatggcctt ttgcggctag atctctccgg caatcttcca
      241 gaagtttttc gattttttca gccactgccg tgtctgttcc tgaaccttta attctggcaa
      301 cgatcaacgc gtttgtgccg tcttcggaaa tcaattgccc ctgataaaac agcgccttgt
      361 tggcaggcag cagggcggac atctgtttga gaatgacact ggaaaatcca agcgggtctt
      421 tggtcatcat gtcgccgcgg ccaattcctt caagctgccc aagggcttgc cggttttgcg
      481 ccatggcctc tctgatgtta tccggcgtca gcagcggcgc aattttctgt tccagatcat
      541 cagcacttaa aagtgcgggc aggttatcgt tgacatgagc gataagttcg ggaaagtttt
      601 cagcgtcgtc cccgatgccc acccgaataa acaggccgct cttgttgagc tggtcggtga
      661 ccgcggccgc agtgtcaatc agcttgttgc ggtcgggaga tgtctgttca agattaatga
      721 acaccttatc ctgaacgggc aggtgtttga taatcagacg cgcgtctgcc aatactgggt
      781 ctttatgcgg catggattcc agaatatccg tatcgatttt aagatttctg gattcccaga
      841 cagaaagccc tgtgacaatg agcgcaacga caaaaataat ggaccagttt acctgatatt
      901 tgcggatgag cggcatgaac acctttattt ttcatttagt tatagatact taaggctgat
      961 tgtgtaaatc atattttgcc ggtcgtggca agataaaaag gcggcgtcgt aatcttgcag
     1021 cgccgtacgg gtcacgtttc cacgcctgat aaggttatgt caagggagca tcgtttattt
     1081 aagaaaaaaa ttttgaaaaa tcagatgcgt ggagattgaa aaatgtacgg ctatttgtaa
     1141 atgaatatat aattcaggca gaataaagcc caacctatta tattctattg agcaatgctt
     1201 ttcagcaccc ggggcaatcc attgccaagc ccaaaacgac ttattggtct gatcccatct
     1261 ccattggatt taaaataatg cagcattttt gtatgttttc tcataccttt catgacgccg
     1321 acaaagccgc aaagcagaaa accgttttcc gttcttttaa tattggattc gcttatccca
     1381 aacgtccgag gatgcacaaa ttgaatgaaa agcctgatga ctttctttcc cgcccttccc
     1441 accgggtaaa cggtattttt ctcaaaatcg ccatacggct cattaaaata atccttattt
     1501 cgggaagaat aacgtattga caggtgctcg cccggataca ctatctgatg ttctttttcc
     1561 tgaacgtgcc tccaaaacca ccatctgacc attttccttt ttacatcggg ccttccggtt
     1621 tccatcgcca cgagatatcc tccattcttc agtttgacaa aaccaatctc atgctgcagc
     1681 gtttctttct caaatattcg attgacgtct tgatattcca ctttgctgat ttgcggccgg
     1741 catatgtgca tgtcctctat gcccgtgtca agatcagggt ttttcatttt ctcgcagcaa
     1801 gaataatctt cttttttcat gatgcttgct tttgccgtat tgagtttgtg ttgacaatca
     1861 aaaagtttag gtcacgctct gacacaaatc aagaacgatg agcaatgaga cttttgaaaa
     1921 gaaattattc aggatgacga tggacgtttt tggatgttat cgtggcggaa atatccgggt
     1981 gaaaatcaga cgggtgaacg ttgaggaatt tcggcagaaa tccacgaacg ggcggaaatg
     2041 agaaattctt cccccttccg gattatagcg gacacggatg ggcgcttcaa taactgggat
     2101 gccgtttctt tcggcgtgca ccagaatttc cacttcaaat tggaagcgcc gtgcctttgt
     2161 tttcagattg agcgcttcgg gcaaaggata aatgcgaaat ccgctctggg aatcggaaat
     2221 ggccggtcca ccggaggccc gcacccagaa attggaaaac ttttttccaa aactgcttgt
     2281 ccagggcaca tgtttgccgg ccatgccttc tctggctccg acaacgatcg gccgtgtatt
     2341 tttgggaata gccttgataa gcttttttgc atcttcagga tagtgctgcc catcggcatc
     2401 gactatgatc gcccaatcgg ccattttcgc agctgcagtg aaacctgtaa ggatggccgc
     2461 gcccttccct tgattttgtt catgttgcag gagttgaatg tctttgattt cttttatctg
     2521 attatacgtg tcgtcagttg agccgtcatc aactacaaca acaggatagc caagtttttg
     2581 agcatcctta gccacctgcg ctaccgttcc ggcatgatta taaacgggaa tgacaaaagc
     2641 aaaacggttt ttgttataat cttttgacct ggtcaatgtt tgtttctcat tttaacggat
     2701 gttgatttga gggagaaacg ccacccccca cgttcccggc cccatgtgcg cgccggacgt
     2761 cagcgacagc ggctgaagta taatttcagc atcaggatac agttccttta tattttttcg
     2821 aaccgtgccg tcgacaaatc gtctgttgtt ggaatattcc agcatgatca gcgcacggga
     2881 atcgtctttg atcgttgcgt caagtttagc caaggcaaac gtgatctgat cctcctgatt
     2941 tttggccgtg ccgacttttt ttgcgccgtc cggcagcgga gatatcaccg gctttacgtt
     3001 gaatttgtcg ccgaaaaagg cgctggtttt ggaaaggcgt ccccccgccg ccagataatg
     3061 caacttgtcc agaaacagat attcttcgca caggcgtatg gccttacggg caaaaataat
     3121 ggtgtttgtc acgtcacggc tttcggccag ataccgggcg gtggacaaag cgatggttcc
     3181 cagtcggccc gatgccgctc ccgtatccaa gatgatgaac cggtcttctt tgtcgtgatc
     3241 ttttttccac tggacggccg cctgataatt gccggtaaat acggagccga cgcaaaggta
     3301 caacacttta tcaaaacgct ccagcgcgct ttgataaaac tgatgccgtt cataaacaga
     3361 ggcctgggac gtggataccc ggatgtcctt cttcatggcg cggtagaggt cttcgggatg
     3421 aaaaaaggtt tccggcaggc attgatcgcc gatagtcaga taactgttta acagggtaaa
     3481 accatggatc ttggcgtcct gagtcgtcag ggaaccggcc gcatcggtta taatatgcaa
     3541 tgtcttttct gcatttaaat gcctgaaatc cgctgtttga caactcaggt cgtcatcttc
     3601 ccaggtcacc aaagagccga gccctgttat ttgagagcgc acctgctcct tgtcctgcgt
     3661 gtgaagatga accttgtaaa actccccttc gggaatgacg atcacttctt cctgcacgct
     3721 ggtgatcatt tttatttcat cgggagaata gcggggcgcc ttgaccacaa aatcaacgca
     3781 gtaaccggat tctatgtttt cctgaaaaga cgatgagatg tgcagccgat catggaatat
     3841 ttccgtcaga ggacgaccat cttcggaaag gcctgtgagt gtgtaaaaga atccttcaaa
     3901 aaaaatatag attcccagcg cgcccgcatc gacaacaccg gcctcctgca atttaggcag
     3961 ttgctcatag gtttgatgga ccgcgttttc catgcttcgg atgatgtttt caatgttttc
     4021 tttatccggt gtgaattgtt ccgacagtac gtcggccagg gcgtcaaaaa cattcagcat
     4081 ggtcccccga cgcggattga ccagggcctg ccaggccttg atctttcctt cacgggcacg
     4141 tccggccagg tcggcgatgt tttccaccgc gcagaaggcg gagaaaaact gtgaggcgat
     4201 gttaccggaa ttgccgcgcg ccgacagaag cagccgatga atcatcgttg cttgcggcag
     4261 atcggtgttg cgaaggggca tcagactaag ggaaagattt ctgccggtgt cgccgtcggc
     4321 aaccggaaaa acattgatgg aatccagcag atcggagcgg gccataagac gttccacacc
     4381 ggccgtgaag gattgtttga gcacctcatt catttcagaa accaaatgcc cggctgattt
     4441 cttcgggaat gcgaaacagc atttctcttt ccggaacttt tacggtgacc tgttccgtgc
     4501 gtccgcgggc aacaatcttc ccgtcctccg cttttcggat ttcaaaatcg acgatcagtt
     4561 taatgcgggc ttcaaccagc gtcgctttgc agataatttc atcgcggtga aaaagcggca
     4621 aaacgtgttt ggtcgaggtt ttgatgatcg gaaaaatgag ctgatgcttt tcataaaacg
     4681 aataaagatc gatgccgcgg ctttcgaaaa gcgccgcacg ggcgatttca aaatagttca
     4741 gatagttggc atgccagacg atctgcatcg gatcgagatc aaaaaaagga actctgattt
     4801 ttacgtcgat actgttttgt ttttctttca tggttttatt ttaaaaaagt ccgaatatcg
     4861 aatggcggtg cagatatttt caatatcttt atccattgcc ctgtcttcaa tgaccggcgc
     4921 gctgacggat ctgatcttcc ccatggtttt cgacagcagc ggtcttgcgt tggtgttgtc
     4981 tcggatgtcg caggcctggg ccgccgcgag aagatgaatg gcaacaacgc gttccgtcaa
     5041 cgtgcagatt ctttcggcat cgcgcgccgc gatggtgccc atgctgacct tgtcctgatt
     5101 atgcgattcg gtggaccggg aaaatgacgc tgccggcatg gttaatttca gtgcttccgc
     5161 cgtcagcgcg gatgatgaaa tggacatggc cttgaacccg tgccgcaacc ccttttcgtg
     5221 tggcggcttt ctcaccaggt cgccgggcag ccctctgttc agattcggac tgaccagaag
     5281 catgatctgg cggtcgcaca tatcggcgac agaagccagc gccgctttta gtccgtccat
     5341 ggcgaaggcg atgtgcccgc cgtaaaaatt gccgcccatc aggatttcac cggtcgcggg
     5401 atcgaaaatg gggttatcgt tggagctgtt ggcttcgatt tccacccatg tcctgatcca
     5461 ttcgaggccg tcatacagga caccggccac ctgcggcgtg cagcgtagag agtaaggatc
     5521 ctgcaaggca tcgagggaat cgtcttccag gtgccgcgtc gaaatttttg ccttcaacag
     5581 gcgggtgatc tgagcggcca caaatgcctg accgggaaaa ggctttgctt ccgaaatcac
     5641 gggatgataa tgccgggcat tgcctttgag cgcatgaatc gaaagagccg tggcgtaaat
     5701 cgcagccgat aaaatcctgt aggctctttc gatcgtcaaa gcggcgatgc ccgtcatggt
     5761 cgttgtgccg ttgagcatgg ccagaggttc tttgggaaga tacgtgtagg gctttagccc
     5821 cgcctttttc atggctctgg ctgcggacat gacggtgccc cggtaaatga cgtcgcgttc
     5881 gcccatcagc gtggcggcga tataggacat cggcgtcaga tcgccggatg cgccgaccga
     5941 accttcacag gggacgatcg gcgtgattcc gtgatttaga aaatcggcga tctgctgaag
     6001 cagtgcgatc gacacgccgg aatagccgcg cgccagacag atcatacggc agaccatggc
     6061 cgcccgcgtt tcttcgacgc cgatgggttc tcccgtgccg caaccatgaa aacgaagaat
     6121 gtttacgccg ttcttcaagg caatgtcaaa gggcatgcgc ttaccgcagg acttgccgta
     6181 accggttgtg acgccgtaaa tgggaatgcc tgtgcgcatg gattgcatga gcatgtcccg
     6241 ggtttttttc atcttacgga caaaggtcgc atccttgctg attttaactg caacgccgta
     6301 gcgcgctacg gcgatgatgt cgttgaaacg gattttgccg ccgtcaatga tcacggcgct
     6361 cctgttgacg ttttttttca tgaactatga aacccctttc ttgtcccgaa taacctttgg
     6421 acgaacgcgc ggtttgtatg tgccgtccgc cttttctttg tccatcactt ttttcaacag
     6481 tttgaacatc ttgtaattgg ccggctcctc ctcatagaat ttatcccagg cgtaataata
     6541 aagctcctga agttttcccg gcgttaagac tttgggctga aaaacaactt tcccgcaggt
     6601 atagtcgctc cagtcgtagg agaaaatccg cccggcattg tgcagatcat cgaaagtccg
     6661 tgtatgggga aagggcgtca ggatggtaaa ttccgccatg tccagttcaa tgtccaggag
     6721 aaaatccacc agctttttga tgtagtcttc cgtatgataa tccagtccca gcagaatgct
     6781 gccttccacg ccgatgccga aatcgtgata acgcttgatg cggtcgcgga taaaatcgga
     6841 cgtatcgaaa accgtctgat aaacaaacca ggctccggcc tgcgcggcca gatccagaac
     6901 ggcgtcgtct ttttcaatcg ggtgacagca ccacttcttt ttcagcggga tcatttcctt
     6961 gaataactgt gtttcccagt ttttatcctg cgccagcgaa ttgtctacaa tgaacagccg
     7021 gttgttttca atgctggcca tttcttcgac gactttatcg aacgggcggg gacggaattg
     7081 cctgccgccc agaaatcgca ctgcacaggg atagcagtta aaccggcagc cccgggacgc
     7141 atgaaccaga tccaccatct gcacaccgcg gtaattgtac atgtcgcgat ccagaatgct
     7201 gcggcgggcg gttccgacgc tttcaatcgg cggaaagttc tgcatgtagt tgtatacctt
     7261 ctgcaggttg ccgtttttaa aatcatcgaa gacctgctcc atccggcctt cggcctctcc
     7321 gagaaaaacg ctgtcggcgt gttgctgggt ttcttcggcg tgcagcatgg tggcgatgcc
     7381 gccgaaaatc accttcattc ctttttcacg gaatcggtcg gcaatgtccc aggcgcgttt
     7441 gatctgggca gtcagcatga cggaaatgcc gacaaaatcc gcaggctcat cgaaatcaat
     7501 atctttgatg ttttcgtcga tgaactcaac gtccacatcg tcgggcaggg tggcggcaaa
     7561 caccacgggt ccgtgcggcg gaagggtaaa tcgcgtctgt ctgtccagct tctcccattt
     7621 cggataaatc aatttaaact tcatcagttc actttctgct cggttatttt caatgttctc
     7681 accagatggt gtttacaata ttttatagca tacgccaata gaaaattgga ccgtgaaaaa
     7741 atacggtctt ggtcataatt tgctgaagat taaatagcaa tatgtgccgc cgaaggaaat
     7801 tccggcaagc gcggtatgat gaatgtccgc ttcttttttc tcctgtatga caaattcaag
     7861 cgcacgcacg ggtttggcaa gacccgccgt cggcggcaag ctttgatttg ccatcgacag
     7921 agccgccgcg caggctttga tgccgccgct ggaaaagctc tctcccgttg ctcctttaat
     7981 cgacgtgatg caaggacgtt ttgtttcgtc gaatatttcg gcatacgctt cagcttccgc
     8041 cgcatcgaga attttatcgc cgttagccgc cgcaaaaatt gcctgcagat cgcttatcgt
     8101 gataccggcg tttttcacgg cgcgggcgaa ggttctcttg atgccttccg tatcgcgcgg
     8161 ccattcattg ggcctggtgg gtgacgcgcc catgccggcc cctgaaattt cgcaatagta
     8221 gttgcatccc ctttgttttg cggattgcag actttccagc agaatcatgc cgcagccttc
     8281 tccggcaata cagccgtttc tttcctgatc aaaaggacgg cagatttcat ttcctttatc
     8341 ttgaggcgaa agaacgccga atccgatcag cgagcgatag gcaatctcgg ataaaatatc
     8401 cgatccgccc gcaaagataa aatcggcggt gccccgtctg atttcagcgg cggcgtatgc
     8461 aattgcattt tcggcagaca cggcaaaatg cgtgatggtg gtattgatgc cgcggaaacc
     8521 cagctcaata gatgtatgac cggccggggc gttcatcacc gtgttgggta ccaggatcgg
     8581 attgacggac gcaggaccct cctgtaacag cgtgtgcaga aactggacgg ttatatccgt
     8641 ggcgccgaac gccgttccca gaataatacc gactctgtcg cggttttctg ccgtgatctg
     8701 caatccggcg tcttccagcg ccatccgcgc ggaggcaacc gtcatcaggg agattttgtc
     8761 catccgccgc atatttttca cggcaataaa atcccttggc ttaaactctg tgacttcggc
     8821 ccccagatgc gaagaaaaag caccggtgtc gaatgatcgg atgtctttga tagcggtgtg
     8881 accggcaaac agttttcgac cgaattcttc tttgccgatg cccaaaggtg tcaccatgcc
     8941 gataccgctt acaaccacgc gccgggcatc atcttgcggt cggtcagcgt tcatgacgtg
     9001 cgctcctttc cgtgtatttg ccgaagatca gcgtggtgtt gttgcctccg aaagcgaagg
     9061 aattggaaag cgcggtcttt aaatttgcgg atcttgcgcc gctggtaatg taatcgaggt
     9121 cacactgcgg atcgcgttcc tgatgattga ttgtcggcgg aacgaagcca tcacgcagcg
     9181 cgaggatgga aacaatggct tccagagcgc ctgcagcgcc cagagtgtgc gcgtgcatgg
     9241 atttggtgga actgaccgga atcgtataag cccgcttgcc gaaaacattt ttgatggctt
     9301 tggtttccat ggcatcgttg accggtgtgg ctgtgccgtg tgcgttgatg taatcgatat
     9361 catcgggaga cagtccgctg tcccgaagcg ccgcgtccat ggaccgaacg gcgccggagg
     9421 cctgttcgtc gggggcagtc atatgataag aatcgcaggt gatgccgtag cccagaatct
     9481 ctccgtgaat tcgagctcct cttgccagcg ccgaatccag ctcttccagg atcacgatcg
     9541 ccgcggcctc acccagagac aggccgtccc ggtttttatc aaatgggcgg caggtagcag
     9601 cggcaaccga tttgagtgcg ttgaaggctg catatgtcag ccgacacatc ggctccacgc
     9661 cgccggcgat gacaacggac gcctggccgc cggcaattaa atcacgcgcg tagccaatgg
     9721 cggtggcgcc tccggaacag gctgtcataa acgttgcttt cggtcccgcc aaaccgaaag
     9781 ccgaagcaat acggtcggcg gaagaagcgc agaataaaga agcaagccgg gagaaccggg
     9841 cacgccgtcc gttactcttc aaaaaattcg cgtaaaagga ttctgcttcg agcagtccgc
     9901 ccgcgccgcc gccgatcgat acgcccgtgt ccttctgaag ttcctccggg aaaggatata
     9961 atccggcatc atgcaaagct tccagtgttg cggcgaatga aagcagatct gcgcgcgaca
    10021 ttctcttcat ggagaaattc tggggaatgt agttgcgggc ggcaaaattt ttaatctggc
    10081 cgcctgtccg ggcagaaaat cccgatgtat cgaaaatatc gatttccctg atgccgcaat
    10141 gaccgttttt cagcgctggc gagaaatcgg ggatattatg ggcgatggcg ttgagcgttc
    10201 ccatgcccgt gataacaatt cgtttttgca aattcaaccc tcatctgatt tttacggcga
    10261 ttctaaaaat agatgtaaaa cttaaattga cgtcggtaaa aagtcaaatg aatattcccc
    10321 agtgacgatg attttccctc tttttgcccc gcttccgtct tgcgtttccc caatccggat
    10381 acggttgtcc ggcagggcct tcgggaaacg gtccaaaaaa ccgcttgttt ttttggtatc
    10441 ctgttgcgat aatattagga agactatcat ttgatatttt aattattagg agatcgtgat
    10501 ggccaaaaaa aatcctgaaa caaaatatga agaaaaagtc ttcgtcaaag aaaggatcat
    10561 cgccaaacga ctaaaaaaca gtaaaaagga ggcggctgat caagaaatta aagccgcaag
    10621 aaaagcggaa cgtctcgatg aagaaaacga ggtgctgatt accattcttt ccgaaataga
    10681 aaatcttccc aaagaagata tcctctataa ttacagcgaa gacatttcca tgaccggaac
    10741 ccggattcag ggaaattgtc ttttgcccgt cgatacattt ttgaaaatcg atttagtatt
    10801 gaaaaatttg aaacaaacgg taacggtatt cggcaaggtg aaatggagca agcccgcaga
    10861 agacgtcaag tcttacgagg cgggcgtgga gtttgtggat acgcccgagg agtccatcaa
    10921 aaaactggga gattatatct tgtcgatcaa tcagtataaa aatcttaatc cggtcggggt
    10981 gccctactgg atttttgcaa aattcaacaa accttcatca aaataaattt ttatcggtcc
    11041 gtttcaattt tcaaatccgc tttacatcgc ttttgccaaa aagcatttcg ttcttatctt
    11101 tttgtatgtt tcattaaacg tcttgaacaa tttattaaaa aaatttcgga taacggtctc
    11161 agagaaaaaa tccggccgat gtcaaaagac aaatatttta ttatttcaat gacatagaaa
    11221 ataacgatcc tttcacgcaa actggcacga taatttcaat aaatataggc aacaacgcag
    11281 gaaaggaggt aacagatcat gaaaaagaca ttaagaacaa tagcggtggc agcggcggtt
    11341 gttattttta ctgcaacgtt cggctttgcg gagtacgcgg caacaggcgc ggctgatttt
    11401 ccctatttcc aactggggtg cctgattgtc ggcgggttga tcttgatgac tctgaaaagg
    11461 aaatatgaaa aaatgtatgt tgccgaaatg gttggtgcgt ttgctcttta cacgcttttg
    11521 atggcattgt tcaccaatcc ggttatcgat gcagttaaaa ctatggttat gtagcacgtg
    11581 ttaaaaagat ttgaaggaac gaaaacagaa tgtaaataac cgctatcaac aaaaatcatg
    11641 atctgttttt atagtggttg cccccttgaa tcggaagatt caagggggtt tttaatgatg
    11701 ttgattgagg attgtccttt atcggaaaaa aagttatagg gataaaaagt tcgtctgaaa
    11761 aacgaacaat tattgacaga tctcatggat gaatcgggtt gatcatgaat tttctggccc
    11821 tggattttga aaccgccaat tattatcagg acagcgcctg tgctctcggc ttggtgcgtg
    11881 tcgaagataa caaaatcatc caccggacat cctttctgat caggccgcca taccggtatt
    11941 ttgtcttcac acacattcac ggtattacct ggggcgatgt tgaagatcag ccgactttcg
    12001 atcagtgctg gaaatcgatt aagccgtttt ttgaaggcat tgattttgcc gtggctcaca
    12061 atgccggctt cgataaaggc gttctgcgcg cctgttgcag aagatatgct atcacgcccc
    12121 cggaccttga ttttcaatgt acgatgaagc tttcccggaa ggtattcaac ctctatccca
    12181 ccaacctgcc cgccgtttgc agaaatttca atattgactt acagcatcac gacgccttgt
    12241 ccgatacgct ggcctgtgca aaaattatga tagaaattat aagaaaaaca aatacttaaa
    12301 aaatactgag aaaatcatca atctaagttg agtaattcct caaatttttg ttgacatgtc
    12361 ccttcaaggt tgctatgagg taaaaaaatc aaaattttat ttttgtgagg gactaagaga
    12421 gtcttcttaa gctattgtta ttttaatgat aaattaaaaa atagctctca ttttaataaa
    12481 tttacataac aagccctctt ttaaaattct gatttgcgca gccggaaccg tcacacgtgg
    12541 tctcggtctt tggtatctat ttgaaaaaac aaacatgaaa tggtgaagcg tccatgacca
    12601 gcaagcagaa tatcagggtg gtgatcaccg gagtcgggtt gacggctccc aacgccaatc
    12661 atctgaacga ttttcgccgc gcccttttaa accagaaatc cggaatttcg gaaatggaag
    12721 tccgatatat gggaaaggtt gccgcgggta actgtaattt tgacgaatat ctttatcaga
    12781 agaaaaaaat gcgtcgccgg ggaacccggg ccggttctat ttcgatttac tgcgcgcacg
    12841 aggcattgaa agatagcggt ctgtccctgg aatcactggt caaggacaga accggcattt
    12901 acctgggcat taccgagcac ggcaatgtgg aaaccgaaaa tgaaatttat gaactccacc
    12961 agaataatct cgatgccggt ttatggtctc atcatcacaa tccgagaacc gtggccaaca
    13021 cgcccgccgg cgaagtcagt ctgaatacgg gaattaccgg tcccgcctac acgatcggcg
    13081 cagcctgtgc agcgggcaat atcggcgtta ttcacggcat gcagatgctg cagctcggtg
    13141 aaatcgatct ggcgttggcc ggaggcgttt cggaaagcac gggaacattc ggcatctttg
    13201 cgggattcaa aagccagggt gccttgggtt ctcatgaaga tcccacaaaa gcgagccgcc
    13261 cgctggacat caaccgaaac ggcattgtgg tttcggaagg cggagccgtt tttgtcctgg
    13321 aaagactgga ggatgccttg caacgcggag cgaagattta cggggaaata gcgggatatc
    13381 atgtcaattc cgatgccgtc gattttgtgc tgccggatac ccaacggcaa attcagtgca
    13441 taaacacggc aatggccaag gcaaacatct ccccccgcga tattgatatc gttaatctgc
    13501 acgccaccgg aacagtgtcc ggagatgaaa acgagtgtga agcggtcagg gcggttttcg
    13561 gacaatccga taacacctac gtcaattgca caaaaggctt tatcgggcat gccatgggcg
    13621 ccgccggcgc tctggaactg gcaggaaatc tgccgagctt caccgatcac aaaattcatt
    13681 cgtgtaaata tattgagaat gtcgatccga agtgtaacgt gaaaaatctg gttaacggcg
    13741 atcccgtcaa aaaagacgtg aactttattt tgagcaattc gttcgggatg ctgggcataa
    13801 attccgtttt aatcgtaaaa aaatacaaac aataaaatta taaggaggca ggggcatgga
    13861 ctatcaaacc ataagaaaaa aaatcatcga tatcatagct gaaattgcga ttgatgaaga
    13921 cctttcaaat ctcgacgaca acatcagatt aagggaacaa ctggatatgg attccatgga
    13981 ctttctggat gtggtcatgg aactgaagaa gagatataga attgaagtgc ctaccgaaga
    14041 ttacatgaag ctggcaacat tgaagagctg tattgaatat ctcggcccca agtttgctgt
    14101 aagtccggca taatgacaca atatgatacg gtaatcatag gcgcgggcat gtccggtctg
    14161 gcggccggca tccggctcgc gatgtttgat caaaaagtct gcatcctgga acggcataaa
    14221 attcccggcg gactgaattc ctactatgac cgccagggcc ggaaactgga tgtcgggctt
    14281 catgcaatga ccaattttgc ccgccagggt gaacggggcc ggccgctgac caaactgctg
    14341 aaacaattac gaatacctta cgaacagctg gaactctcgc aacagaattt ttcgcgcatt
    14401 gtgtttcctg cacacgatct cgcattcgct aatgatccgc agttgctgat acaggacatc
    14461 ggaaaacact ttccccatca gaaagatcct tttgccagac tgcttgacgt agtcatcaat
    14521 tttgatgacg ttaatctgag caacaaaccg caaagcgcca aagaaaaagt tcgggaaatt
    14581 atctccgatg agtatttgct ggaaatgctt ttttgtcctc tgttgattta cggcagtgcg
    14641 tgggaaaacg atatggattt ttcccagttc gtcattatgt tcaaaagtat ttatctggaa
    14701 ggcttcagcc gcccccgaaa cggcgtgcgc accatcatca gtctgctgat tgagaaatac
    14761 aaacaagccg gcggcgaatt gcgtctggga acgggcgtca aaaaaataga tgtggaaaat
    14821 gaaagcgtaa ccggcgtaac gcttgattcc ggagaaagca tccggaccga caaaataatg
    14881 tcctctatcg gcttgcccga aaccatgaac attgtttccg aaaaaagcga caggcctgca
    14941 gtcggcaaca tgagttttgc ggaaaacctt ttattctttg acaaaaagcc cgttgaggcg
    15001 ggcatcaaag aaaccattat tttttacaat gacaatgata cgtacgacta caaaaagtcc
    15061 cgggatcttt gtgattatcg cagtgccgtc atttgcctgc cgaacaattt cggaaccgat
    15121 gattacgatg aaggattaat tcgaatcacc tttattgcca attttgatca atggaacgcg
    15181 ttggacagaa aaacatatct tcaaaagaaa aaggaagtat gtaatgcggc tttgtcgctt
    15241 gttgcaaaag tgattccgca atttcaggcc aagctgcttt atcatgatgt tttcacaccc
    15301 aagaccatta accgcttcac cggtcatttc aagggcgcgg tttacggcac aaccgagaaa
    15361 gtcagagacg ggcgcaccgg cataaaaaac ctgtttatct gcggcacgga tcagggcttt
    15421 ctggggatcg tcggcgccat gctcagcggt atttccatgg cgaatcttca ttgtctgatg
    15481 aacggagaaa aagcatgagg tttaaaaacg cggtcatctc ttcttttgct tatcatcttc
    15541 ccgaccaggt catgacgtcg gaagaaattg aggagcggct tgcgcctctt tatgaacggc
    15601 tcaaactgcc cgcgggacgg gtcgaactga tgaccggcat taaggaaagg cggttctggc
    15661 cgcccggaac catgcccagc gatttatcca cgcaggccgc ccgtaatctg tttgagaaaa
    15721 acatcgtgca gccggaccag attgatttat tgattcatgc ctccgtctgc cgcgattttc
    15781 tggagccatc gacgtcttcg atcgttcatc acaatctgga actgcaaacg gatatcatgt
    15841 tcttcgatct gtccaacgcg tgtctgggcg tcatgagctc gtttgtcgtt gccgctaata
    15901 tgatcgacaa cgggttcatt aaacatgccc tgatcgtgtc cggcgaaaac ggcggaccgc
    15961 tgctttttca gaccattgac cggttattaa atgataaaaa tataaccaga aaaagcatca
    16021 aaaaatatat tgccaatctg accatcggct cagccgctgt tgcctatacg gtttctcaca
    16081 aggatcatgt ccatgaaggt cattgcctgc tgggaggagc ctcgcttgtc gattcttcag
    16141 ccaacgtcct atgtcgcggc ggcggagaca cgcaatcctt gatgatggaa accgattccg
    16201 aggaactgat gaaatccggc gttgccctgg ctgaaaaaac ctgggcagaa accaaacggg
    16261 aactggggtg gacccacgat gacgtgaact gctttgtcgg tcatcaggtg ggtgtggctc
    16321 atgaaactct gttaaagcag attctgaaac tggagcattg tcccacgttt accacgtatc
    16381 aatttttggg caataccgga gcttctgcgc tgcccgtaac actggctatt tatgatgaag
    16441 agaaaaaaat tcccgcaggc agccaggtgg gtttgttggg catcgggtcg ggcctgaatt
    16501 ccattatgct gggggtaaaa tggtaagacc ggactggttg aacaaattat atcctttcac
    16561 atcccatttc tatcaactca aggataatat ttccctgcat tacctcgatg aaggacaagg
    16621 tgatccgatg ctgatgcttc acggcaatcc cacctggtcg ttttactaca gaaaactggt
    16681 ggcggcgttt tccatatcca acagggtgat tgtcccggat cacatcggtt gcggtctgtc
    16741 ggataaaccg cagcaatatg cctatacctt gcatcaacac atcaggaatc tggacaaact
    16801 tgtttcctat ctggagttga aaaacgttgt gctggttgtt cacgactggg gaggccccat
    16861 cggtttcgga cttctaaagc gacgtccgga actggttaaa aaaatagtga tcctgaacag
    16921 cgccgcttac acgtccaagg tgatcgcctt tcagattaat atgtgccgga ttcccatttt
    16981 ggccgaacag gtgattcgcc gtcttaacgg atttgccctg gctgcaacgt acatggctgt
    17041 tgctaaaaaa atgtctcctg aaatcagaaa gggatatttg tatccgtacg acaattacga
    17101 aaaccggatt gccatagcgc agtttgttgc cgatattccc atgacgcccg gacaccggag
    17161 ctatcagacg ttaaaatctg ttgaattatc gcttgcgcaa catcgatgtc ccgtgcttat
    17221 tctctgggga aaaagggatt tttgctttca cgattatttt ctcaatcgct ggcaggaaat
    17281 ttatcctcac gcgaccgtaa aaacatttga ggacgccggc cattatgttc tggaagacgc
    17341 cggagacgac atcattgcag aactcggaaa gtttgtttga ataaaattgc atgacggtga
    17401 aaagctgtaa ccatgaacat cgctgatcgt ttcgaagaga tagcaaaaat tcatcacgac
    17461 aaagttgcgg tcaagtttcc cgttcgcacg ggcaaaaagt accgctacaa agccatcacc
    17521 ttcggggaac tggagacgct tgccggcaaa tacgccaacg gtctttctga aatcggattt
    17581 cgtcgcggct gcaaagccct tctttttgtc cggccgtcat taaaatttcc cgcgctggtt
    17641 tttgccctct ttaaaatcgg cgtcatcccg gtgttcattg atcccggcat gggcagaaaa
    17701 aatctcctgg cggcgattga agaagtagct ccggaagggg tgattgccga accggaagtg
    17761 catctcctga ggattctttt caataactcg tttaaatcgg tcaggttcaa agttaccacc
    17821 aaaggaccga agataggcaa ttgcttcccg ctgtccatgt tgaaacgggc cagagccggt
    17881 tttcaaagcg cgcagctgga accggacgaa acggcagcca ttctgtttac ctcgggcgga
    17941 accggctgcc cgaaaggcgt tgtctatacc cacaaaattt attacgagca gactcgtctt
    18001 ttacaggagc ttttttctct cgatgaaaat gaaatagatt tcccctgctt cccgctgttt
    18061 tctctcttta cgctctgtat gggcatgacg tcctgcattc cggatatgaa tccctcgcaa
    18121 ccggcaaagg cggacccgaa gcaattggtg caaaatatca aggacaataa agttaccttt
    18181 atggccggat cgccggctat ctgggaagcg ctgggaagtt actgcattaa aaacaacatt
    18241 acccttcctt cggtgaaata tctggtgatg ttcggtgcac cggtcagaaa cgaactgcat
    18301 aaaagattca gcaggattct gcccaacgga accacctata cgccttatgg cgcaacggag
    18361 agtcttcccg tagccaatat ttccggacaa atggttttgg agcaaacggc cgcgctcacc
    18421 gatgaaggca agggaacctg tgtgggagag cctgcccccg gcgtgaaagt tgccattgcg
    18481 gcgataaacg acaacataat tcctcatatc cggcaggcgc gcatattggg cctgcatcag
    18541 atcggagaaa tcattgtgaa aggcgacatg gttaccgccg ggtattatca atctccgcag
    18601 gagactgccc tggcaaagat tgccgacggc gcatcgtttt ggcacagaat gggcgatctc
    18661 ggttatattg atgatgcagg caagctctgg ttctgcggga gaaaatcgca ccgcgtcgaa
    18721 acaccgtcgg gattattatg ctcagtcatg tgcgaagcga ttttcaacac gcataacggc
    18781 gtcagacggt cggcgctggt cggaatcgga gaaaaaggca ggcaatcggc tgcgatcgtc
    18841 attgaaagaa attcggcagg gcgaaagctt gacaggaaac gattggaaaa agagttattg
    18901 ggtctgggcg gaaagaatcc tttgacagcg gcaataacgt cagtccattt ctgcgacagc
    18961 tttcccgtcg atgtccggca caatatcaag atcgaccgtt tgaaactggc tgatgacgtt
    19021 gcccggggga ggatatgaac attatcgtta ccggagcggg aggttttctg ggaagaaatc
    19081 tggcaaacgg cttgctgtcc aaaggacaca aggtccggaa cttcagccgc aggaatcatc
    19141 tgccattgca ggccatgggc gttgaaacta tttgcggcga tttgcaaaac gcttccgagg
    19201 ttatggcggc atttcaggga atagatgcgg tctttcatgt tgcatcgcgc gtcggtatct
    19261 ggggcgacta tgatgaatat taccggacga atgttgtcgg cactggaaat gtcatcaagg
    19321 cgtgccgcgc caacggcata acaaaactga tttatacaag cacgcccagc gttgttttcg
    19381 acggcaatga tattttgaga gccgacgaga ccaaacccta tgccctgaaa ttttataatg
    19441 actattcccg gaccaaaacg ctggccgaac agaaggtttt gcaggctaat gatgaaacat
    19501 tatctactgt cgccctgagg ccccaccaga tcatcggtcc ggacgatccg catctcgtcc
    19561 ctcgactggt caatgcggca aaacggggca gactgaaaat agtcggaacc ggcaagaatc
    19621 ttgtggatgt cacctatatt caaaatgccg ttgaggcgca cctcctggcg ttcgaaaagt
    19681 tgtctcctca atcatctgtt gccggacagg cttatttcat cggccaggaa cggccggtcg
    19741 tgctctggga ttttatcaac aagattctga cgcgatataa tctttctccc atcacaaaaa
    19801 gagttcccga aaaagtggcg ttgaacatgg gggccttgtt cgaagcctgg tacagatttt
    19861 ttaaaattaa cggagagccg ccgatgacgc gatttgtggc gctgcagttt tcccgctccc
    19921 attatttttc ccatgaaaaa gcgaaaaacg atttcggtta tgtcccgaaa atatccatag
    19981 agcaggcgct ggatcttaca acagagaggg gaatgaatgc ttttaaataa aaacgataaa
    20041 attacacaac actacgatgt catcatcatc gggggcggac tggggggatt gaccgccgcc
    20101 aacaaactgg ccaaaaacgg aagaaaagta ttaatcctgg agtcgcacga taaacttggt
    20161 ggctttgcta cctggtttta tcgcaagaaa catatttttg atgtgtcgtt gcatggattt
    20221 ccggtgggga tgatcaaaac ctgccgcaag tattggaaca aggcnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnngttttc tccaatttca tgagcaaagg cgtttatacg
    20401 tttcagggcg gaaccgacct cgtcatcgga atgatgcagg aggagcttat gaaaaataat
    20461 gtcgatattc gtctgaaatc aaacgtggga aaaattgtgg ttgaaaacaa tgtttgtcgg
    20521 ggagtcatgc tcggcggcga gtttgtttct tccacgtcgg ttctttccaa cggcaatctc
    20581 atcgggacca tttttaatct gacgggagaa gataatttta aaccggaatt tatcgacaag
    20641 gttaaaaaag tccggctcaa caccagttcc tgtcaggtgt atatgggaat cagacaggga
    20701 gaagcgattg aggatatcgg cgatctgatc ttctactcgg aagcaaagga atttgaaacc
    20761 gatgagttgc tgtccatgaa caccaccagt cggacattct ccttttacta tccgcgactg
    20821 cggccccaac tggaaaatgc cggatatacc atcgtggcat cgaccaatgc gcgctatgaa
    20881 gactgggcag aattaagcaa agaagattat aagcaaaaga agcgggaatt gattgaggca
    20941 acgctggtga gtctggaaaa aattattccc ggcatccgac agaaaattga ctacgtcgag
    21001 gcggcaacgc cgttgacgat caagcgatat acgcatcatg aaggaggctc ctcgttcgga
    21061 accaaacacg agggcttgga tatttccatg aaactgcatc atgaaatcag cggtctgttt
    21121 cataccggat cggtcggcat tatcatgtcc ggctggctgg gcgccgccaa ttacggcgtc
    21181 attcaatctt atgaagtgga aggctatctg gataaactta attgaggtgt tttatgtttg
    21241 atttcagcaa tcaaagagtt attgttaccg gcggaacccg tgggatcgga gcgggtatcg
    21301 ccgaggcttt tctgcaggct ggcgcgacgg tgattgccgt ttattcagcc aacgatgcgg
    21361 ccgcgaatga atttaaaaat aaaaatgaac aattttcgga tagactcgat attcaaaaac
    21421 aggatatctc ctcgtcgcag gatgttaata atttgtttca gtatctggat gaaaaatatc
    21481 cctcattgga agtgctggtg aataactcgg gtatccggca ggacgcgata gcggcgttaa
    21541 tgaccgatga gcaatggaat aaagttttgg aagttaatct gacaggaacg ttttatatgt
    21601 cgcgggaaat tattccgcgc tttatgtccg gtcgtttcgg acgaatcatc aacatcagtt
    21661 cgctgggcgg ggatatcgga tttcaaggac aggccaatta cgcggcatcc aaagccggcc
    21721 aactggccat tgccaaatct ttgtccaaag aggtcgccag gaaagggatt accgtcaact
    21781 gcgtttctcc gggatttatc gatacggaac tcatcgggga tctcccagag gagcagcgca
    21841 aggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttcg cccgtccggg
    21961 cgatacgttg accatgaagg ttacccttga tgatcaggtg gataacgctt attatatgag
    22021 cggtacgatt catatcggag ataaactcat tttaaaaatc atctttcagg cggcgcttct
    22081 tccgccggag caataagaga ggttgatatg gactttctgc aagtatcagg aaaaacattt
    22141 ctggtgaccg gtgtggccaa taaaaagagc gttgcgtttt ttgtggccaa aggcctgatc
    22201 gaaaacgggg cgcaggtgat tttttccgtt caaaatgaag aaaataaaga cagagcgcaa
    22261 aaacttttcc ccgacagcac cgtgttgatc tgcgatgttg aaaaaacggg aagcatcaat
    22321 cgtctgggag aatattttaa aaatcataat atcaaactcg ccggttttgt tcattccatc
    22381 gcctttgcca attacagcga aggcctcaaa ccgtttcatg aaaccaaacc ggaagatttt
    22441 ctgcaggcgg ccaatatctc ctgcttctcg ctggtggccg tcgccaatgc cctgaagaat
    22501 tcttttgaaa acgatgcttc cattgtcacc atatccatca gcaacacgcg ggcaaccgcc
    22561 tatgggtaca tgggaccgat caaggccgcg cttgacgcga cggtggcttt tctggcaaaa
    22621 tccttatccg aaatgtccaa agcccgtgtc aatgcggtat gcgccggtcc tttgaaaacg
    22681 tcggcctccg cgggtattcc gggatatgtc aacaattatc tgtttgccga gcagttgacg
    22741 ctgcgcaagg ctgcgcttaa aaccgaagaa gttgccaaca cggttttgtt cctgctgtcg
    22801 cctgcctccg gcggcattaa tgccactggt gttgtggtgg atgcgggaat gtcttgtaat
    22861 tatttcgacc aggatgtagt aaataaggcg atgcgttgat tgaaaggaaa atagtaaaaa
    22921 tgactgtccc gctcatatcc atcgtcggta aatccaattc gggcaaaacc accctggtgg
    22981 agaaactgat tcccgaactg attaaaagag ggtatcgcgt cgcgaccatc aagcacaaca
    23041 agcacggttt tgatatcgac catgaaggca aagacagctg gcggcacaaa aaagccggcg
    23101 cctgcgcgac cgtgatcgcc tgtcccacgc gtgttgctct gattgaagat cttgaacggg
    23161 atcagacact cggtgaaatc agggataaat atatcaagag cgccgacgtc attcttgccg
    23221 aaggttacaa gggcaaccct ttccctaaaa tcgaagtctt ccgttcggaa ttgaaacgcg
    23281 aattattatg cggcaaggaa gacaacctgc tggc
//
LOCUS       DAOSME010000034        23246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2756_length_23246_cov_21.950498, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000034 DAOSME010000000
VERSION     DAOSME010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23246
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_23246_cov_21.950498"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLG98_06555"
     CDS             complement(<1..455)
                     /locus_tag="PLG98_06555"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE32952.1"
                     /translation="MAHIWPIGGGKGGSGKSFVASSMGRLLAKSGKKTLLIDLDLGAA
                     NLHTMVDVPYPEKCFSDFVSKKIHDLEDTVLQTPFPNLFLISGAHDSLDIANLPYEKK
                     IKTLKAIVKLDYDYIILDLGAGTAFNTLDFFLASRNGIFITTPEPTSIE"
     gene            758..2077
                     /locus_tag="PLG98_06560"
     CDS             758..2077
                     /locus_tag="PLG98_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOE32953.1"
                     /translation="MIHYEPEIRVALLQNYSEAHITLKGDFSSAHGRILEGCYTVTVD
                     DGWVLLADSSGKEIVRQKEILLQAGSRSFFTVSDVKIGIDFHWQRTQEQSFRGDLFLS
                     ASRDASFNLINNVLLENYLESVISSEMSAEAPLEFLRAQAITARSWLVSMLAKKQEAR
                     TSTPQRTGNEIVVWQDVNDHDGFDVCADDHCQRYQGITKIISENVHKAIESTRGHFLV
                     YGGKICDARYYKCCGGQTDVFDTAWEDKHYDYLSNISDDVKPHSPVCTEGDARRWLTG
                     RPKAYCNTTDEKLLKQILPAFDRETSDFFRWQVAYEREDLEEIIREKSGIDFGDLKNI
                     EPLERGPSGRISRLKIDGSKRTVMVGKELEIRRWLSPSHLLSSAFVVTVRRNTYGEVI
                     GFVFIGGGWGHGVGLCQIGAAVMASKGFQAEEILTHYFTGAQLKKLY"
     gene            2208..3524
                     /locus_tag="PLG98_06565"
     CDS             2208..3524
                     /locus_tag="PLG98_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE32954.1"
                     /translation="MSETITETTRNAVRRNPWLWVPSLYLAESIPYTMAMTVSVVLYK
                     DMGLSNTQIAFYTSWLYLPWVIKPLWSPFVDIFRTKRFWILAMQLAIGASFAGVALTL
                     PTTDFVRYSLAVFWIMAFSSATHDIAADGFYMLGLDTPQQAAFVGVRVIFYRIATIAA
                     EGGLVFLAGMLQKYGYPAVGAWSLSFIVVAAVFLSFFIYHIFALPKPAEDISAPWDKS
                     KNFLAEYFRVLTLFFRRKDILIIITFFLLYRFAEAQLTKMVVPFLRDEISKGGLGLST
                     ADIGIIYGTVGVIALMLGGLLGGYVIYRNGLKYWLWPMVFIMHLPDLIFVYLSHYQPQ
                     NLWLVGSAVAVEKFGYGFGFTAYTMYMIMVAQGSYKTVFYAIGTGIMALGMMIPAMGS
                     GWIQDMLGYKNFFIWILITTIPGFIVAALVKIDPEYGKKINKEPRG"
     gene            3531..4247
                     /locus_tag="PLG98_06570"
     CDS             3531..4247
                     /locus_tag="PLG98_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOE32955.1"
                     /translation="MQNKQLNTAFILGAGLGTRLRPLTENTPKPLLEIGGHPMITYAM
                     AHLRAVGIKRFIINTHHCAEKYVEAFPEGNWQGTPITFRHEPVLLDTAGGIKNIEDLI
                     AEDERIIVYNGDIIANMPIELLIRKHFELKTSVTLALRSTGPLLNVSVDKDGFVCDMR
                     HILKNDGVKKCLFAGIYIVEKSFLKRLTTGKIESIVLPLVEMIKENPRSVGGVIIDDG
                     GWYDVGTVAEYEKLSKTDIR"
     gene            4352..5344
                     /locus_tag="PLG98_06575"
     CDS             4352..5344
                     /locus_tag="PLG98_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOE32956.1"
                     /translation="MQKEIQKFLTEHLGKTDFKLESIKKGGSDREFFRVSLPHGDSFI
                     FMRYGDEVAENANWAGINQFMASMDINVPRIILRDIPRHFILIEDLGDVDLWSLRFEP
                     WEERRNHYFQVLAQIHRLHSFDLKSTSPDVQLSESYGPRLYKWEHNYFLENLAWEVCK
                     IKLTSADAAKLNKELDALFESLQKIEPSLIHRDFQSQNIMIKNERPVLIDFQGMRQGC
                     LFYDLGSLLCDPYVTFEEKERDELLDFYYDIIRPAYSRDEFAHNFWAGSVQRLLQALG
                     AYGYLGLKKHKPAFLHHIPNGLENLLTAVDNAGGLKMLNDLATECETIIARRNI"
     gene            complement(5419..7212)
                     /locus_tag="PLG98_06580"
     CDS             complement(5419..7212)
                     /locus_tag="PLG98_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOE32957.1"
                     /translation="MVLHKIKQIVGWLRSFFLENITRWLVFGLLVGIVSGLGASAFYY
                     LLQAGRHLCFHLLAGYAVPAPAGEQVFDVPGILQFHPLIFFLLPALGGLISGLLVYHF
                     APEAEGHGTDAMIDAFHNKKGRIRTRVPVIKGLASIITLSSGGSAGREGPIAQIGAGI
                     GSAVARLLKLSNRERRILLLAGCGGGLSAIFRAPLGGALTAIEILYKEDMETEALVPT
                     VVSSITAYIIFTYFFGNTPIFFFPEYTFSNPRELIFYVLLGLICVPAGIFFIKIFYAV
                     RERIFLPLKIPRYVKPALGGLVVGCIGLVFPQVYGDGWGWIQLAILGKLSIGIMIAIA
                     LAKIVATSFTISSGGSGGVFGPTLFIGGMIGGTVGFSAHYFYPHIVTQPEAFVVVGMA
                     SFFAGVASAPIGTLLMCSEMTRGYGLIAPLMLVSIIAILFTRQHSIYTKQVQSKRESP
                     AHMAEFTVNLFAETQVKDFYKPEKVTPVREITTYGQLKRIFSASNVECFPVYNEDDVL
                     IGAINWDHARSIVFEEGLEDLVIAQDLMAPPQTVTPEDNFYDAFVKFMETNVEELLVV
                     SSDDPNRVLGVLRHDDLIAAYHAELNRRKYE"
     gene            complement(7660..8532)
                     /locus_tag="PLG98_06585"
     CDS             complement(7660..8532)
                     /locus_tag="PLG98_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE32958.1"
                     /translation="MIIDCHYHLEERVFTVERLIGEMQKAGIDKVALMGSIIGPFTEP
                     PRILINLLQRLLENSFSRSIGKAFVSNFTDHGDVRILGKPYPLATDPDNEKVFNAVRK
                     YPDKFLGWIFVNPRGKNNQVEEYEKYKDEKGFIGVKAHPFWHHFAPVELAPVAERLAK
                     TGKPLLIHAGFGEEGNYEALLKKVPDLKLVLAHAGFPCYSDTWKKIKPLKNVYMDLSQ
                     TTYTSGKATRQAVDMFGAERLFFGTDGPFGFHGPDHQYDYGFIKRRIEGMFADVIIRE
                     KLLGGSFAKVVGLS"
     gene            complement(8569..10593)
                     /locus_tag="PLG98_06590"
     CDS             complement(8569..10593)
                     /locus_tag="PLG98_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOE32959.1"
                     /translation="MNYRADTLCGLFHNQALRYGDRFVFLTGRFEADGRASDKFSSRT
                     WKQVREEAIALAKGLMALGIKKKDKVVIYSESRPRWIIADQAVQACRAIGVPLYPTVS
                     EEELDYMLEDSESCLAIVSTPEKARRVLKVTAGKAKMPVIMMSPWDDVGQKPEGVYTF
                     PEVFALGDPKVKLDVIEEGIRSVVPEDVASIIYTSGTTGKQKGVILTQSNWIHSMHQC
                     SGSEIMALTAQKDLHLKALVHLPLCHVYGRMSDYHTAGLKMGGELVFAESYQTIARDL
                     REVRPNIINSIPRLYEKTYEIVQSTLSRAKKPYQAIYNWAMSKGRIYVDCMATGKRMP
                     PHQLMLFGLANSLVFDRLKKEMCMDKLVMACSGGGKLSKDICIFYRALGIQLIEGYGL
                     TETTAINNLNAPEIMMEKPPTGFLKVLYDRIMALTLHLMVVRQSQGKSPYANPVMSLL
                     LSLCYNTLVYRLRVKPGFVGRVVPETEMRIAEDGEILIRGPQVFQCYWKREKDTADAF
                     TPDGFFMTGDIGVADEEGFLQITDRKKELFVTSGGKNVAPHPIEVALIERPYIDQACL
                     IGDGRKYLTAFIIPDFESLKRYAKDHGITCASQEDLANHPDIKALITKEVDHVNATLA
                     RYEQIKYFTILGRAFDVASGELTPTMKIKRRVVNEKYQQQIEEMYGKPNQ"
     gene            complement(10697..12190)
                     /locus_tag="PLG98_06595"
     CDS             complement(10697..12190)
                     /locus_tag="PLG98_06595"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOE32960.1"
                     /translation="MRTKEQYIKGLRKMKRNLYFNGSKIDRDDEEQLPALNVMGFTFD
                     APHIPEFKDLCTAKSHLTGETINRFCHVHQNSDDLHRKQDMTRALCRTGRMCIQRCMG
                     VDAINGVNAASYEADKQNNGSTEYHKNFLKWLENFQRNDLVGCCAQTDMKGERLKRPA
                     EQTDPDMYLRVVGKKKDGIVVRGCKLHISEASIADEIIVVPTRILGPDEQQFAVSFAV
                     PADYDGVKQVVHIHKIRKRTHFKTGNPDIGYTDSYVIFDDVFVPWERVFLCGEYVHGG
                     ALAMLFALFHRHSYSGCKPAIGDMLLGTASLAAKMNGISKAHHVREILSRIIMVTELG
                     YAAGFTASAKSKPEIYVPGIGQMPYGPGSYIPHSIYANVGRCLTGEAVFDEQEMLCNI
                     AGGLPSTFPYEADLANPELKPLLEKYLKRNPKIPIADQIKFWLLFAEATCSSSTGFMC
                     YGSYHGGGSPIMEQIAITLQYDTKLREHLVNAAAGIEKLDYGRVTKI"
     gene            12460..13164
                     /locus_tag="PLG98_06600"
     CDS             12460..13164
                     /locus_tag="PLG98_06600"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE32961.1"
                     /translation="MARHDIKEARKDNLIDAALRVFAEKGYSDTTMTDIARKAGVSTP
                     VLYEYFKTKEDLLFAIPEKFMEEPIRIMEYVAPYLRGAEAKIRGIVQGYLTVYDENPL
                     YASLVMLELKTNRKFLKAKAYGMVRKVARGLLEVIEEGIKDGTFKKDTDAFLVRSMLL
                     GTIEHLCIRKLLLGEPKNLPQYTDAIVNIILNSIKKESRQFSVNLNINDLLSPNIKTE
                     NIPANKGTSDPKRKKR"
     gene            complement(13217..16900)
                     /locus_tag="PLG98_06605"
     CDS             complement(13217..16900)
                     /locus_tag="PLG98_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOE32962.1"
                     /translation="MISENIRRLVKTVVHFAGRCHIYVGRRPDHVARPSLIFFPVLDG
                     YLCCGFAGLMTCGLKSQPPQTRADLTLVALWKKLKSANVKNPSRLPDVTETVNAMADT
                     VAQLKKEEAQEFLFFHPDYSSDLSVFAADLNRFIAEKENRLDEQTAFLTSAELESVNS
                     LIVMLKDIGWALEKDILANLPKILALTGAGNRADIRPAAFRKYRKLNLALNALDRLEV
                     RGRDSAGIDLTFAPESENDLKAALQRITEQGWLADYEKRTQPGDLLNLSVSVTKNTRG
                     TNAGIVVTFTYKTFSTVGKLGRNVAELRNSIRNDRILRCFADLETSCENVLMHTRWAS
                     VGSITEENCHPVNNHSMIRFACPSTGYPESDVQIHVVLNGDVDNYTPLRQFLETQGEM
                     IARPITTDTKIIPLQIEKFLHSGHTLTEAFRRAVNDLAGSHAIAMTSHLEPGKMFLAL
                     RGSGQSLYIGVSDDQYMFSSELYGLVEVMPRFLKMDGEAGGQICILDQNGPAGVRGIT
                     SYSYDGKILALTENMLGTAEITTRDIDRGDYPHFFLKEISESSLSVKRTLRGKYRMTA
                     TEAALQQVRFNLGEDIVPADIIAGLKKGRIKNIVIIGHGTAAVAGQAVADAFSLYLGS
                     SPLHITARVASELSGFGLKDDLSDTLVIPITQSGTTTDTNRAVAMAKERGAHVISIVN
                     RRQSDITAKSHGVFYTSDGRDIEMSVASSKAFYAQIVAGQVLALFFAQSLRTCTDEDI
                     IACLRNLESAPLLMNQIASDCASVASTVKDAIGKKYWAVTGSGPNKAAADEIRIKLSE
                     LCYKTISTDIVENKKHIDLSAEPLILVCTAGSPESVTADIVKDVAIFKAHKANVIVIA
                     DEGEKRFQGVADAVIAVPVAFPPLPVILNAMAGHLWGYYAACAIDEEAQIFRAFRARL
                     HDILMLKAREQLSVFDLTADVSVKGIINTFYQSFNTRRQQGAFSSLEGKTLSDLMLLL
                     KYAAGKLPLKDMALDFVNDQGIFSPFELLDTTLGTVIDELSRPIDAIRHQAKTVTVGT
                     SRKERPPEGIVFDLLKTLTFDIATLTYRDIMMIRRIQPVIASIPGYTIYDISALDQQG
                     NPTDDSLIAVRVKGGLAAGMKSRADKPAVLMGVKRTIVSTGHAYLGRGKTDNAPILII
                     PLRTQEAGIGNLLLLHVRFDEKLTLQEKIDALGYRYNDIRNLIDEYNLKWDDRFIDRF
                     SLEALFSEPIEIIAGRIKSQLNP"
     gene            complement(16908..19517)
                     /locus_tag="PLG98_06610"
     CDS             complement(16908..19517)
                     /locus_tag="PLG98_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOE32963.1"
                     /translation="MTPYARVSTGTSSLDDIINGLQMGDNVVLQVDDIEDYKKFVEPF
                     VKTSLASRHRLVYMRFAAHPPLLEANKKIKVYKLNANNGFESFSTQVHNIIKEEGRDV
                     FYVFDCLSDLLMAWATDLMIGNFFVITCPYLFELNTVAYFAILRSSHSFKTVARIRET
                     TQVLLDIHNSNGQLCVHPLKAWKRYTPTMFLPHIMENDKFVPILNSADAASFLSYLTN
                     KNTTGGVRNLDYWDRLFLKASNLLEDPDAVLDRQEMVDSLSRVMIGREKRMLSLVKEY
                     FSLEDLLEIKKRLIGTGFIGGKSVGMLMARNILRKDPAFDWDSELELHDSFYIGSDIF
                     YSYMVQNGWWKLLMAQKTDEGYFEVARELKSRMLHGIFPDEIKEQFQLMLEYFGQSPI
                     IVRSSSLLEDAFGNAFAGKYESYFCPNQGTPETRYKNFEDAVRKIFASTMNEDALTYR
                     VQRGMAHQDEQMALLVQRVSGSHRNRYFFPDIAGVGLSYNTFVWQKGMDPKAGMLRIV
                     FGLGTRAVNRVENDYPRIVALDAPLIKPHAKQEDIRRFSQNEVDVLNLKDNHFQTLPT
                     SDLLDYAIEPHIGMIAVRDAAVDEYYQSLGREAKETWILTFDELLENTPFARTMSDML
                     KKLAQVYRYPVDIEFTANFNAEGKPKINLLQCRPFQTKGHYSRVELPEKIEKSRILLR
                     QEANFMGGSVYQAISRIIYIDPRGYARLTVSEKHEVARLTGKLNKQIGKREKMPTILF
                     GPGRWGTTTPAMGVPVTFSEINNIMAIGEIAYQDGALIPDLSFGTHFFQDMVEMDIFY
                     MAIYPEKEGVFFNTSWMAKQPNILADLMPEDGRFAKVVKVFDMRNQDLRLLSDIVTQK
                     MICFFGDASAIGK"
     gene            20039..21271
                     /locus_tag="PLG98_06615"
     CDS             20039..21271
                     /locus_tag="PLG98_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOE32964.1"
                     /translation="MDSGNTAWVLTASALVLLMTIPGLAFFYGGLVRRKNVLSIMMQC
                     FVIVCVISLQWMLFGYSLAFGPDFHGIIGKLDWMGLAGVGATPNPDYSGGIPHYAFMI
                     FQAMFAIITPALIIGAYAERVKFSAFLVFTLLWATFVYDPLAHWVWGVGGWMRTLGAL
                     DFAGGIVVHVSSGISALVLALMLGKRIGYNHKPIRPHNLPFTVLGAALLWVGWFGFNA
                     GSALAADGLAANAFVTTHIATAAAGITWALIEWWHNGTPTILGAATGAVAGLVAVTPA
                     CGFVNPMNAMFIGMIVTVVCYIAVAVIKGKLGYDDSLDAFGVHGVGGTVGTIATGLFA
                     EKVVNAAGADGLLFGNAHQLYVQSLSLIVTVAFAAIMTFIIFKLVDALIGMRVEENNE
                     VVGLDLTQQSEAAYTVIE"
     gene            21284..21625
                     /locus_tag="PLG98_06620"
     CDS             21284..21625
                     /locus_tag="PLG98_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOE32965.1"
                     /translation="MKLVIAIIQPHKLEAVKRELEAVDVNLMTVTNVLGQGRQKGVTE
                     IYRGAKEAGGLLNKVRLDIAVNEDFVEPTVKAITKGAHTGSVGDGKIFIVELAECIRI
                     SSGERGGLAIG"
     gene            21636..21839
                     /locus_tag="PLG98_06625"
     CDS             21636..21839
                     /locus_tag="PLG98_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE32966.1"
                     /translation="MSIRIHKAKTNALARCVSECEKAVIMHVLLKTKGDQSEAASLLG
                     IKESFLADKIHQYEIDCARFELK"
     gene            21861..22781
                     /locus_tag="PLG98_06630"
     CDS             21861..22781
                     /locus_tag="PLG98_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32967.1"
                     /translation="MKDNLKTGIIMVLLTLILALLPVMSWADEEIVPADPKQGDNAST
                     EVKPVEQDQPEDKVTGEVAASVLSAYIWRGQELTRHSVVIQPSVTAAYKGFAANVWGN
                     LDTKPYSALDVDYSSNFTETDVTLSYLHKFGILQAGIGYIYYGLAGAVQGGADLPDSQ
                     EVFVMLGLDTILAPTLTVYKEFDYYNQWYATLGISHTFALHEKVGLKFAAQASYLRSD
                     DEATYPEFDSASLATTDKFNNLHDGVVSVSLPIAVTGHLTISPVLTYVFPLSDDAKYE
                     MKARGLKGDIDPSHKEGDFLYGGIVVSYAF"
     gene            22830..22903
                     /locus_tag="PLG98_06635"
     tRNA            22830..22903
                     /locus_tag="PLG98_06635"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22862..22864,aa:Gln,seq:ctg)
     gene            22964..>23246
                     /locus_tag="PLG98_06640"
     CDS             22964..>23246
                     /locus_tag="PLG98_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32968.1"
                     /translation="MRAHYFQHVPFEGLGSIESWLKKSGFKITSTRFYESAKLPDLKT
                     IDLLVVMGGPMSVNDEDRFPWLVAEKQFIWDAIAKSKPVLGICLGAQLIA"
BASE COUNT         5767 a         5601 c         5868 g         6010 t
ORIGIN      
        1 ttttcaatgg acgtgggttc cggcgtggtg ataaagatgc cgtttcttga ggccaggaaa
       61 aaatccagcg tgttaaaagc ggtacctgcc cccaaatcca gaataatgta gtcataatcc
      121 aacttgacaa tggccttgag agtttttatt tttttttcat agggaaggtt ggcgatgtcc
      181 aggctgtcgt gggcgccgct gatcagaaaa agattaggaa agggtgtttg aagaacggta
      241 tcttccagat catggatttt tttggagaca aaatcagaga aacacttttc cgggtaggga
      301 acgtccacca tcgtgtgcag atttgctgct cccagatcaa gatcaatcag cagcgttttt
      361 tttccggatt tggccagaag acgccccatc gaacttgcca caaagctctt gcctgatcct
      421 cctttgcctc ccccgattgg ccaaatatgc gccataaatc atccaatgag taatttatga
      481 aagccataca cgataaaaca atgatattca agcaattagt tccttcatct gacaattttc
      541 cgggaaatta aaaaagtcgt tgcccataac gtccgcatag tccgggagtc gtcaggcgat
      601 cactgtgcgt tgaatgattc cgcacgcggc gtcatggaaa gtttctcgtt cgttgaatgg
      661 cctgatgttg cggagggcac gggaatgtcc cggagagcac gatggggaat acattccttt
      721 cttgacttct ctttctttta ttcataaaaa acaaaccatg atccattacg aacctgaaat
      781 cagagttgcc cttctgcaaa attacagcga agctcatatc accttgaaag gggatttttc
      841 atctgcgcat ggccggatat tggaaggttg ctataccgtt accgttgatg atggttgggt
      901 cctgcttgcc gattcgtcag gaaaagaaat tgtgcggcag aaagaaatcc ttttgcaagc
      961 gggaagccgt tcttttttta ccgtatcgga tgtaaaaatc ggcattgatt tccactggca
     1021 gcggactcag gagcaatcgt ttcgcggcga tttgttttta tccgcttcac gagatgcttc
     1081 tttcaatctt atcaacaatg ttcttctgga aaattatctg gagagtgtca tctcttcgga
     1141 gatgtccgcc gaagcgcctc tggagttttt gagagcacag gcaattactg cgcgaagctg
     1201 gctggtgtcg atgcttgcca aaaagcagga agcccggaca tcaacacctc agagaaccgg
     1261 aaatgaaatc gttgtctggc aggatgtcaa cgatcatgat ggttttgatg tgtgcgccga
     1321 cgaccattgc cagcgctatc agggaatcac aaaaataatt tcggaaaatg tccataaggc
     1381 gatagaaagt acccgcgggc attttctcgt ttatggagga aaaatttgcg acgcgcgtta
     1441 ttacaaatgc tgtggcggtc agacagatgt tttcgatacg gcgtgggaag acaagcatta
     1501 tgattatttg agcaatatca gcgatgatgt gaagccgcat tctcccgtgt gcacggaagg
     1561 cgacgccaga cgatggttga ccggcaggcc gaaggcttat tgcaatacaa ccgacgaaaa
     1621 attattgaaa caaatcctgc cggcttttga tcgggaaacg tccgattttt tccgctggca
     1681 ggttgcctat gagcgggaag acctggaaga aatcatcagg gaaaagtcag ggattgattt
     1741 cggagatctg aaaaatatcg agccgctgga acgcgggccg tccggcagga tctcacgcct
     1801 gaaaatcgac ggctcgaaaa ggacggtgat ggtcggaaag gaactggaaa ttcgccgctg
     1861 gctttcaccg agtcatctat taagcagcgc ttttgttgtg actgttcggc gtaatacgta
     1921 tggagaagtt atcggttttg ttttcatcgg cgggggctgg gggcacggcg tggggctgtg
     1981 ccagatcggt gcggcggtga tggcctccaa gggatttcaa gcggaagaaa ttctgacgca
     2041 ctacttcacc ggcgcgcagt taaaaaaact gtattgataa gccgtaattg aattatcagt
     2101 gcgagtgagc cgtccttcag atggtgttgt agaataatat taagacttca tcgcggcata
     2161 gctggactgt agctttcagt tgaagtgaca taaaaaagag aaaggaaatg tccgaaacaa
     2221 taacagaaac aacaagaaat gcggtccgga ggaatccctg gctttgggtg ccttctctct
     2281 atttagctga aagcattccc tacacgatgg ctatgacagt cagcgttgtc ctatacaaag
     2341 atatggggct ttccaacacg cagatcgctt tttacaccag ttggctttat ctgccctggg
     2401 tgattaaacc gctctggagt ccgtttgtcg atattttccg taccaagcgc ttctggatac
     2461 tggcgatgca attggctatt ggcgcgtcgt ttgccggtgt cgccctgacg ctgccgacaa
     2521 ccgattttgt gcgctactct ctggctgttt tctggattat ggccttcagc tccgccacac
     2581 acgatatcgc cgccgacggt ttttacatgc tgggactcga cacgccgcag caggctgcat
     2641 ttgtcggtgt gcgcgttatt ttttaccgta ttgccaccat tgccgctgaa ggcggtctgg
     2701 tgtttctggc cggtatgctg caaaaatacg gttatccggc agtcggcgct tggtcgctat
     2761 cctttatcgt cgtggcggcg gtttttctct cgttttttat ttatcacata tttgcattgc
     2821 cgaagcccgc cgaagacatc agcgcgccgt gggacaaatc aaaaaatttc cttgcggaat
     2881 atttccgcgt tttgacctta ttcttccggc gcaaggatat tctgatcatc atcacctttt
     2941 ttttgcttta ccgttttgcc gaggcgcaac tgacgaaaat ggtcgtgcct tttctgcgcg
     3001 atgaaatcag caaaggggga ttgggcttga gcacagcgga tatcggcatc atttacggta
     3061 cggtcggcgt cattgcgctg atgctgggcg gtcttttggg cggttatgtg atttacagaa
     3121 acggtttaaa atattggctg tggccgatgg ttttcatcat gcacctgccg gatttgattt
     3181 ttgtgtattt gtcccattac cagccgcaga acttatggct ggtcggttcc gccgtggcgg
     3241 tcgaaaaatt cggttacgga ttcggtttta cggcctacac catgtacatg atcatggttg
     3301 cacagggcag ttacaaaacc gtgttttacg ccatcggtac aggcatcatg gcgctgggca
     3361 tgatgattcc ggcgatgggc agcggctgga ttcaggatat gctcggctac aaaaatttct
     3421 tcatctggat tctgattaca accattccgg gttttattgt tgcagcgctg gtgaagattg
     3481 atcctgagta tgggaagaaa atcaacaaag aaccacgcgg gtgaaactat atgcaaaaca
     3541 aacaactcaa cacagcattt attctagggg cggggctggg aacgaggctc aggccgctga
     3601 cggaaaatac acccaaaccg cttctggaaa tcggcggaca tcccatgatt acttatgcaa
     3661 tggcgcatct gcgcgctgtc ggtatcaaac ggtttattat caacacgcat cactgcgcgg
     3721 aaaaatacgt tgaggctttt ccggaaggca attggcaggg cactccgatt acgtttcgcc
     3781 atgaaccggt tctgctcgac acggcaggcg gcatcaaaaa tatcgaagac ctgattgccg
     3841 aagatgaacg aattatcgtt tacaacggcg acatcattgc caacatgccg atcgaactgc
     3901 tgattagaaa acattttgag ttgaaaacgt ccgttaccct ggcgctgcgc tcaacaggtc
     3961 cgctgctcaa tgtcagtgtt gataaagatg gctttgtctg cgacatgcgg catattttga
     4021 aaaatgatgg cgtcaagaaa tgtttgtttg ccggtattta tattgtggaa aaatctttcc
     4081 tgaaacggtt aacgacggga aaaatcgaat cgattgtttt accgctggtg gagatgataa
     4141 aggaaaatcc acgctctgtc ggcggtgtta tcattgacga tggtggttgg tatgatgttg
     4201 gtactgtcgc tgaatatgaa aaattgagca aaacagatat tcgttaaatc tcattcaaaa
     4261 ttgtcatgcc ggcgaaggcc ggtatccagt atttttcagt tttggattag ccgctcagac
     4321 cgggtcatga caacattatt aagtgaagct catgcagaag gaaattcaaa aatttttaac
     4381 tgaacatctg ggaaaaaccg attttaagct ggagtcgatc aagaagggcg gttcagaccg
     4441 tgaattcttc agggtgtcat tgcctcacgg ggacagtttt attttcatgc gctacggcga
     4501 tgaggttgcg gaaaatgcca actgggccgg catcaatcaa ttcatggcat ctatggatat
     4561 caatgtgccg cggattattc tccgggatat ccctcggcat tttattttga ttgaggatct
     4621 gggtgatgtt gatttgtggt cgctcagatt tgaaccctgg gaagaaagac gcaatcacta
     4681 ttttcaggtg ttggctcaaa ttcatcgtct gcattctttt gatctgaaat ccacttcgcc
     4741 cgatgtgcaa ttatccgaaa gttacggtcc aaggctttac aaatgggagc ataactattt
     4801 cctggaaaat ctcgcatggg aagtctgcaa aataaaacta acatctgcgg atgccgcgaa
     4861 actgaataaa gagctggacg cgcttttcga gagcctgcaa aaaatagaac cgtcgctgat
     4921 tcaccgcgat ttccagtcgc aaaacatcat gattaaaaat gaaaggcctg tattgattga
     4981 ttttcagggc atgcgtcagg gctgtctttt ttacgatctc ggatctcttc tctgcgatcc
     5041 atacgtcacc tttgaagaga aagaacgaga tgagttgctg gatttttatt atgacattat
     5101 tcgtcccgcc tatagccgcg atgagttcgc tcataatttc tgggcgggtt ccgtacagcg
     5161 gcttttacag gcgctggggg cgtacggata tctgggactc aaaaaacaca agcccgcatt
     5221 tcttcaccac attcccaacg ggctggagaa tttattgacg gcagtggaca acgcgggggg
     5281 gcttaaaatg cttaatgact tagcgacgga atgcgaaaca atcattgccc gtagaaatat
     5341 ttagaattaa tctactattt tttagaagaa catgtccgca ggacgacaaa aaagattttt
     5401 ttatcaatcc attcatgcct attcatattt tcgtctgttc agctccgcgt gatatgcggc
     5461 aatcagatcg tcgtgcctta aaactcccag cacccggttg ggatcgtcgg aactcaccac
     5521 cagcaattcc tctacattgg tttccatgaa ttttacaaac gcgtcataaa aattgtcttc
     5581 cggcgttacg gtttgcggtg gtgccatcag gtcctgcgca atgactaaat cttccaggcc
     5641 ttcttcaaag acgatggacc gggcgtggtc ccaattgatc gccccgatca gcacgtcatc
     5701 ttcattgtac acgggaaaac attcgacatt ggatgcagaa aaaattctct tcagctgtcc
     5761 gtaagtcgtg atttcacgga caggggtgac cttctccggc ttgtagaagt cctttacctg
     5821 ggtttcggca aaaaggttca cggtgaattc cgccatatgc gcgggcgatt cacgcttgct
     5881 ttgaacctgc tttgtataga tggagtgctg ccgtgtaaaa agaatggcaa tgatggacac
     5941 cagcatcaac ggtgcaatga ggccgtatcc tcgggtcatt tccgaacaca ttaacagtgt
     6001 tccgatgggc gcactggcca cccccgcgaa gaatgacgcc atgcccacaa ccacaaaggc
     6061 ttccggctgc gtcacaatat gcggataaaa ataatgggcg gaaaatccta cagtgccgcc
     6121 gatcattcct ccgataaaca gtgtcgggcc gaaaacaccg ccggacccac cggacgatat
     6181 tgtaaaactg gtggcgacaa ttttggccaa ggcgatcgct atcatgattc cgatgctgag
     6241 tttccccaag atggccagtt ggatccagcc ccagccgtcg ccgtaaacct gaggaaagac
     6301 aaggccgatg cagcccacaa ccagaccgcc cagagccggc ttgacatagc ggggaatctt
     6361 caggggcagg aaaatacgct cccgcaccgc gtaaaatatc ttgatgaaaa aaatcccggc
     6421 ggggacacag atcagaccca gaagcacata aaagatcagc tcgcgcggat tggaaaacgt
     6481 atactccgga aaaaagaaaa tcggcgtgtt gccaaaaaaa tacgtaaaaa tgatgtaggc
     6541 ggtaatggaa cttaccaccg tgggcacaag ggcttccgtt tccatgtctt ccttgtaaag
     6601 gatttcaatg gccgtgagtg cgccgcctaa aggagcacgg aaaatcgccg atagacctcc
     6661 tccgcagccc gccagaagaa ggatgcggcg ctcgcggttg ctcaatttta aaagacgcgc
     6721 aacggccgaa ccgatgccgg cgccgatctg ggcaatcggt ccttcccttc ctgcactgcc
     6781 gccgctggaa agcgtgataa tagaggccaa tcccttgata acgggcacgc gcgtccggat
     6841 gcgccctttt ttattgtgaa acgcatcaat catggcgtcc gtgccgtgtc cttctgcttc
     6901 cggtgcaaaa tgatacacga gtaagccgct gatcaaacca cccagagcgg gcagaaggaa
     6961 gaaaatcagg ggatgaaatt gtagtatgcc ggggacgtcg aaaacctgtt cacccgctgg
     7021 tgcgggaacg gcataacccg ccagcagatg aaaacaaaga tgacggcctg cctgcagaag
     7081 ataataaaaa gccgaagctc ccagacccga cacaataccg accagcagac cgaacaccag
     7141 ccagcgcgtg atattttcca gaaaaaaact tcgcaaccag ccgacaattt gtttaatttt
     7201 atgcaagacc atcaaatcac ctgatgggag aaagtcatgt tttcccgtag agattttttg
     7261 caacttatcc cgaattattc tccgatgcaa acgaaaagtc tgatcgattt cccgccggac
     7321 cgtttcataa tatgcacaag acctttacac gatgatcatt ttctttcgtc tccggcattc
     7381 atctatctgc ggcggcggtt ctgcgccaaa agcggatatc acacgtcatt cgtgaggatg
     7441 tattcaggac cggataggca aaacggtgtg aatgacatgc ggaagagtca aaagttttgg
     7501 attcctatca agtaacggcg tgtgaggcgg cctggatgtc ttgtaagcag aaaggcggca
     7561 gtcggaatcg gatcatccag agacctggac accgaaggat ggcgcggttg atccaccaga
     7621 tgtttttttg tcattcattt gaaatcatgt caacatttct taactcagac cgacgacttt
     7681 tgcgaagctc ccgcccaaaa gcttttcccg gattatgaca tctgcaaaca tgccctcgat
     7741 tctgcgcttg ataaagccgt aatcgtattg atgatcgggc ccgtgaaagc caaacggacc
     7801 gtccgtgccg aaaaaaagtc tttccgcacc gaacatatcc accgcctggc gggtggcttt
     7861 cccgctggta taagtggttt gagaaagatc catatataca tttttcaggg gcttgatttt
     7921 tttccacgta tccgagtaac aggggaaacc ggcgtgggcc agaaccaact taaggtccgg
     7981 cacttttttg agcagagctt cataatttcc ttcttcgccg aatcccgcgt gaatcagaag
     8041 aggcttgccg gttttggcca gcctctcggc aaccggagcg agttccacgg gtgcgaagtg
     8101 atgccagaac ggatgcgcct tgacgccgat aaatcctttt tcatctttat atttttcata
     8161 ctcctcgacc tgattatttt tgccccgggg atttacaaaa atccagccca gaaatttatc
     8221 cggatacttt ctgacggcat taaatacctt ttcattatcc ggatccgtcg caaggggata
     8281 tggctttccc aaaatcctga catcgccatg gtcggtaaaa ttggaaacaa atgctttccc
     8341 tatacttcgc gagaaagaat tttccagtaa gcgttgtaaa agattgataa gaatgcgagg
     8401 aggctccgta aatggtccga taatgctgcc catgagcgca accttgtcga tgccggcttt
     8461 ctgcatttcg ccgatcagac gctcaactgt aaaaaccctt tcttccagat gataatgaca
     8521 gtcaatgatc atgctttttc tccataataa acaataaact gattttattt attgattcgg
     8581 tttgccgtac atctcctcaa tctgttgctg atatttttca ttgacgaccc ggcgcttgat
     8641 tttcatggtc ggcgtcagtt cgccggaggc aacatcaaag gcgcggccga gaatcgtaaa
     8701 atattttatc tgctcgtaac gcgccagagt ggcattgaca tgatcgactt ccttcgtgat
     8761 aagcgccttg atatccggat gattggccag gtcctcctgt gaagcgcagg tgatgccatg
     8821 atccttggca tagcgtttca ggctttcaaa atccgggatg atgaaagccg tcagatactt
     8881 gcgtccgtcg ccgatcagac aggcctgatc aatgtacggt ctttcaatca gggccacttc
     8941 aatcgggtgc ggagccacat tcttgccgcc ggaggtgaca aacagttcct tcttgcggtc
     9001 ggtgatttgc agaaagccct cctcgtccgc cacaccgata tcgccggtca tgaaaaatcc
     9061 atcgggcgta aaggcatccg cggtatcctt ttcccgtttc cagtagcact ggaacacctg
     9121 cggccccctg atgagaattt cgccgtcttc ggcgatgcgc atttccgtct cgggaacaac
     9181 acgtccgacg aagcccggct tcacccgcaa ccggtaaacg agcgtattgt agcagagcga
     9241 aagcagcagg gacatgaccg gattggcata cggcgatttg ccctgcgact gccgcaccac
     9301 catcagatgc aatgtcaggg ccataatcct gtcatagaga accttgagaa aaccggtggg
     9361 tggtttctcc atcatgattt ccggcgcgtt gagattattg atggcggtgg tttccgtcag
     9421 gccgtaacct tcaatcagtt ggatgccgag cgctctgtaa aaaatacaga tatctttgga
     9481 cagttttccg ccgccggaac aggccatcac cagtttgtcc atgcacattt cttttttgag
     9541 acggtcgaaa accaggctgt tggccaagcc gaaaagcata agctgatgag gcggcatgcg
     9601 ttttcccgtg gccatgcagt ctacataaat tctgcccttg ctcatggccc agttatagat
     9661 cgcctgatag ggtttcttgg ccctggaaag ggtggactga acgatttcat acgtcttttc
     9721 ataaaggcgg ggaatgctgt tgattatatt cggacgaacc tcccgcaggt cacgggcaat
     9781 ggtctgatag ctctcagcaa aaacaagttc accgcccatt ttcagtcccg cggtatgata
     9841 atcgctcatt cgtccgtaga catggcaaag cggcagatga accagcgcct tcaaatgcaa
     9901 atctttttgg gccgtcagcg ccatgatttc ggagccggaa cactggtgca tggaatgaat
     9961 ccagttggat tgcgttagga tcacgccttt ctgcttaccg gtcgttccgg acgtgtaaat
    10021 aatcgacgcg acatcttcgg gaacgacgct tcttatcccc tcttctatca cgtccagttt
    10081 caccttggga tcacccaatg caaatacctc cgggaaggtg taaacgcctt caggtttttg
    10141 accgacatca tcccaggggc tcatcataat cacgggcatt ttggctttgc ctgccgttac
    10201 tttaagcacc cgtctcgctt tttccggagt ggagacaatc gccagacagg actcgctgtc
    10261 ctcgagcatg taatccagtt cttcctccga aacggtcgga taaagaggaa cgccgatggc
    10321 gcggcaggcc tgaacagcct gatcagcgat aatccagcgc ggacggcttt cggagtaaat
    10381 cacaacctta tccttttttt ttattcccag cgccatcagc cctttcgcca gagcaatggc
    10441 ttcttcgcgt acctgtttcc atgtccgcga actgaattta tcgctggcgc gtccgtcggc
    10501 ttcgaaacgg cccgtaagaa aaacgaatcg atccccgtaa cgcagagcct gattgtgaaa
    10561 aagtccgcaa agcgtgtccg cacgatagtt catcacaaac ctcctttaat aaaagaattt
    10621 gtttcccttt cttgtgtgcc gctgaatcaa aaggtgttta gggttgcccg accggctgcc
    10681 cgggcaaaaa atttatctaa atttttgtca cacggccgta atcgagtttc tctatgccgg
    10741 ccgcagcatt caccaggtgt tcgcgcagtt tcgtatcgta ctgcaaggtg atggcaatct
    10801 gctccataat cggagagccg ccgccgtgat aagacccgta acacataaat cccgtactgg
    10861 atgaacaggt tgcttccgcg aacagcagcc agaatttgat ctgatcggct atcgggattt
    10921 tggggttgcg tttgagatat ttttccagaa gcggtttcaa ttccggattg gcgagatccg
    10981 cttcataagg gaatgttgaa ggcaggccgc cagctatgtt gcagagcatt tcctgttcat
    11041 caaaaacagc ttctccggtg agacatcgac ctacgttggc atagatggaa tgcggaatat
    11101 aactaccggg accatagggc atctggccga tgccgggaac ataaatttcc ggtttgctct
    11161 tggcggacgc cgtaaatccg gccgcgtaac ccagctcggt aaccataatg atcctggaaa
    11221 ggatttcccg cacatgatgc gctttcgata tcccattcat ttttgccgcc agcgaagctg
    11281 tgcccagcag catgtcgccg attgccggtt tgcagcctga gtaactgtga cgatggaaaa
    11341 gcgcaaaaag catggctaaa gcaccgccgt gtacatattc gccacagagg aaaacccgct
    11401 cccacggtac aaacacatca tcgaaaatca cgtaggaatc ggtataaccg atatcggggt
    11461 ttccggtttt gaaatgggtc cgcttgcgaa ttttgtgaat atgaacaacc tgtttgacgc
    11521 catcgtaatc tgccggcacc gcaaacgaca ccgcgaattg ctgttcgtca ggaccgagaa
    11581 ttctcgtcgg cacgacgata atttcatcag caatggatgc ctccgaaatg tgtagcttgc
    11641 atccccgaac gacgattccg tctttcttct ttcccacaac acgcagatac atgtcgggat
    11701 cggtctgctc ggcgggacgt ttaagacgtt cccctttcat gtcggtttga gcgcagcagc
    11761 ccaccagatc atttctttga aagttttcca gccatttgag aaagttttta tgatactcgg
    11821 tactgccatt gttctgtttg tccgcttcat aacttgccgc attgaccccg ttgatggcat
    11881 ctacgcccat gcagcgctga atgcacattc tgccggtgcg gcagagcgca cgtgtcatat
    11941 cctgttttct atgcaggtca tcggagtttt gatgaacgtg acaaaaccgg ttgatggttt
    12001 cgccggtaag atgagacttg gcggtgcaca gatccttgaa ttcaggaata tgcggagcat
    12061 cgaacgtgaa tcccatgaca ttcaaggcgg gaagctgttc ctcatcatcc cgatcgattt
    12121 tactgccgtt gaaataaaga tttcttttca ttttccgcag gcctttgatg tattgttctt
    12181 ttgtcctcat cagacgctcc tttcggtcat gtcttgtatt tgcggtatat aattatcaac
    12241 gtttaaaata tatgtataaa tattaattca tgataattaa atgtcaagaa atatttattg
    12301 acattagtta ttaggtgaat taatattatt aacataaata ataattgata tttggttgag
    12361 aggtgttttg ggcgatcccg gcataaaagt ccatgaatgt gcttgtgttt tgacatgatc
    12421 tgttttagat aagcggcact gaacggataa ggagaggcta tggccagaca cgatatcaag
    12481 gaagcaagga aagataatct tattgatgcc gccctgcggg tatttgccga gaaaggttat
    12541 tcagacacaa ccatgaccga cattgccagg aaggcaggcg tgtccacgcc cgtgttgtat
    12601 gaatatttca agaccaaaga agatttgctt tttgccattc ctgaaaagtt tatggaagag
    12661 ccgatacgca tcatggaata cgtggctccc tatcttcgcg gagcggaggc aaaaatcagg
    12721 ggcattgtgc aaggatatct gaccgtttat gatgagaatc ctctctacgc ttccctggtg
    12781 atgctggagc tgaagaccaa tagaaaattt ttaaaggcca aggcatacgg gatggtcaga
    12841 aaagtggcta gagggcttct ggaggttatt gaggaaggaa ttaaagatgg aacattcaag
    12901 aaagataccg acgctttcct tgtccggtcc atgcttttgg gcaccattga acatctgtgc
    12961 atccggaagc tgctgctcgg tgaacccaaa aatctgcctc aatacaccga tgcgattgtg
    13021 aacatcatcc tgaacagcat taagaaggaa tcaaggcaat tctccgttaa tctgaatatc
    13081 aatgatttac tgtctcccaa catcaaaaca gagaatatcc cggcaaataa aggcacgtct
    13141 gatccaaaaa gaaaaaaacg ataaaaatgt ttagaactca ttatcggtta atccgggatt
    13201 taccgtcaat acggtactac ggattcagtt gggatttaat cctgccggcg atgatttcga
    13261 tcggttcgct aaatagcgcc tccaatgaaa accggtcaat aaaccggtca tcccatttga
    13321 gattgtattc gtcgataaga ttgcgaatgt cgttgtatct gtatcccagc gcgtcaattt
    13381 tttcctgaag agtcagtttt tcatcgaatc gaacatgcag cagcaggaga ttgccaattc
    13441 cggcttcctg cgttctcaac gggatgatca gaatgggggc gttgtccgtt tttcccctgc
    13501 ccagataggc atgccccgtg ctgacgattg ttcttttcac ccccatcagc acggcaggct
    13561 tgtcggcgcg cgatttcatt ccggccgcaa ggcctccttt cacgcgtacg gcgatcagcg
    13621 agtcgtctgt cggattgccc tgctggtcca gagcgctgat gtcataaatc gtgtatcccg
    13681 gaatcgatgc aatgacgggc tggatgcgcc tgatcatcat gatatcccga tacgtcagag
    13741 tcgcgatgtc aaaggtgagc gttttcagca gatcaaaaac aatgccttcc ggcggccttt
    13801 cttttcggct cgtgccgacg gtaacggtct ttgcctgatg ccgtatggcg tcgatgggac
    13861 gcgacaattc gtcaatgacc gtgcccaggg ttgtatcgag caactcaaac ggtgaaaaga
    13921 tgccctggtc gttgacgaaa tccagagcca tatccttcag cggcagtttg cccgctgcat
    13981 atttcagcag aagcatcaga tcggagagag ttttgccctc cagagaactg aaggcgccct
    14041 gttggcgacg ggtgttgaac gactggtaga acgtgttgat gattcctttg accgacacat
    14101 ccgccgtcag gtcaaaaacg gacagttgct cacgcgcctt tagcattaaa atatcatgaa
    14161 gacgggctcg gaaggcccgg aagatttgtg cttcctcatc aatggcgcag gccgcgtagt
    14221 agccccataa atgcccggcc atcgcgttaa gaataacggg gagcggcgga aacgcaaccg
    14281 gaacggcgat cacggcatcg gcgacgcctt gaaaacgttt ttcgccttca tcggcgatga
    14341 caatcacgtt ggctttgtga gctttgaaaa tggccacgtc cttgacgatg tcggcggtga
    14401 ccgattcagg agaccccgcg gtgcacacca gaatcagcgg ctcggcggac aggtcgatat
    14461 gttttttgtt ttcgacgatg tccgttgaaa tggttttgta acagagttcg ctgagtttga
    14521 tgcggatttc atccgcggcg gccttgttcg gaccgctgcc tgtaacggcc cagtattttt
    14581 tgccgatggc atccttcacg gtcgaggcca ccgacgcgca gtcggacgcg atttggttca
    14641 tcaggagcgg tgccgattcg agatttctca aacaggcgat gatatcttca tccgtgcagg
    14701 tccgcagact ctgggcaaaa aagagcgcca gcacctgtcc ggcgacgatc tgggcgtaaa
    14761 aagccttgct ggaagcgacg gacatttcaa tatcccgtcc atcgcttgtg tagaacacac
    14821 catgagattt ggccgtgatg tccgattgcc ggcggttgac aatcgaaata acgtgggcgc
    14881 cgcgttcttt ggccatcgcc acggcgcgat tggtatcggt tgttgttccc gattgtgtaa
    14941 tcggaatgac cagcgtatcg gacagatcat ctttcagacc gaagccgctc aactccgagg
    15001 caacgcgcgc tgtgatatgg agcggcgatg atcccagata aagggaaaaa gcgtccgcca
    15061 ccgcctgacc ggcaacggcc gccgttccgt gtccgatgat cacgatattc ttgattctgc
    15121 cttttttcaa tccggcaatg atgtcggcgg gaacaatatc ttcgccgagg ttgaatcgga
    15181 cctgctgaag cgccgcttct gtcgcggtca tgcggtattt cccccgcagg gttcttttga
    15241 cggacagaga cgactcggaa atctccttca ggaagaagtg cggataatct ccgcgatcaa
    15301 tatcgcgggt tgtgatttcg gctgttccga gcatattttc cgttaacgcc agaattttgc
    15361 cgtcgtagga ataggacgtg atgccgcgca cgcctgccgg accgttttga tcaagaatac
    15421 aaatctggcc gcctgcttcg ccgtccattt tgagaaaacg cggcataact tccaccagcc
    15481 cgtagagttc ggaggaaaac atatattgat cgtcactgac gccgatatac agcgattggc
    15541 cgcttcccct gagcgcgaga aacatcttgc ccggctccag atggctcgtc atggcgatgg
    15601 cgtgagagcc ggccaaatca ttgacggcac ggcggaaggc ttcagtcagc gtatgccccg
    15661 agtggagaaa tttttctatc tgcaggggaa tgatttttgt gtctgtcgtg atcggccggg
    15721 caatcatctc gccttgggtt tccagaaact gccgcaacgg cgtgtagttg tccacatcgc
    15781 cgttgagaac gacatggatc tggacatcgc tttccggata cccggtagaa ggacaggcaa
    15841 aacggatcat ggaatgatta ttcaccggat gacagttttc ttccgtgatg gagccgacgg
    15901 aagcccagcg ggtatgcatc agaacatttt cacaggatgt ctcaaggtcg gcaaagcacc
    15961 ttaaaatgcg gtcgttgcgt atcgaatttc tcagctcggc aacattccgt cccagtttcc
    16021 cgaccgtgga gaacgtttta tatgtgaacg tgacgacaat gccggcattc gtcccgcggg
    16081 tgttttttgt gacggagaca gacagattga gaagatctcc cggctgagtt cttttttcat
    16141 agtcggcaag ccatccctgt tccgttatcc gttgaagcgc tgctttcaga tcgttttcgc
    16201 tttccggagc gaaagtcaaa tcaatccccg cggaatcccg gcctctgact tccaggcggt
    16261 ccaaagcgtt taacgccagg ttcagtttcc ggtattttct gaatgcggcc ggccggatat
    16321 cggcacggtt tccggcgcct gtcagcgcca gaattttggg cagattggcc agaatgtcct
    16381 tctccagcgc ccagccaata tccttgagca tcacgatgag actgttgacg gattcgagct
    16441 ccgccgaggt gaggaacgct gtctgctcat ccagccgatt ttctttttct gcgataaaac
    16501 gattcaggtc ggcggcgaaa acggacaaat cagaggagta atcgggatga aaaaaaagaa
    16561 attcctgcgc ctcttctttt ttgagttgcg cgacggtatc tgccatggcg ttgacggttt
    16621 cggtgacatc cggcagcctg gatggatttt tcacgttggc ggatttaagt tttttccaca
    16681 gggcgaccaa cgtgagatcg gcccgggttt gcggcggctg acttttcagg ccgcaggtca
    16741 tcaacccggc aaacccgcaa cataaatacc cgtcaagaac cgggaaaaaa atcagagaag
    16801 gtcttgcgac atggtcggga cggcggccga catatatatg gcagcgaccg gcgaaatgaa
    16861 caacggtttt aacaaggcga cgaatatttt ccgatatcat aagtcaacta ttttccgatt
    16921 gctgatgcat ctccaaaaaa acaaatcatc ttctgtgtaa caatatccga caggagccgt
    16981 aaatcctggt tgcgcatatc aaaaacttta accactttcg caaagcgccc gtcctccggc
    17041 atcaggtcgg ccagaatgtt gggctgtttg gccatccacg acgtgttgaa aaaaacgccc
    17101 tctttttcgg gataaatggc catataaaaa atgtccatct ctaccatatc ctggaaaaaa
    17161 tgtgttccaa aagataaatc cggaatcagc gcgccgtcct ggtacgcaat ttcgccgatg
    17221 gccatgatat tgttgatttc cgaaaaggtt accggaacgc ccatggcggg cgtggtggtg
    17281 ccccatcgtc cgggaccgaa cagaattgtg ggcattttct cccgcttgcc gatctgtttg
    17341 ttcagcttgc ccgtaagtcg cgcgacctcg tgtttttctg aaaccgtcag tctggcatag
    17401 cctctgggat caatataaat gatgcgggat atggcttgat aaacgcttcc gcccatgaaa
    17461 ttggcttcct gccttaagag gattctggac ttttcgattt tttccggcag ttccacgcgg
    17521 ctgtagtgtc ccttggtctg gaaggggcgg cactgcagaa ggttgatttt gggttttccc
    17581 tctgcattga agttggctgt aaattcgatg tctaccggat agcggtaaac ctgcgccagt
    17641 tttttaagca tatcggacat tgtcctggca aaaggcgtat tttccagcaa ttcatcaaag
    17701 gtcaatatcc aggtttcttt cgcttcccgt cccagcgact gataatattc gtcaacggcc
    17761 gcatcgcgta cggcaatcat accgatatgg ggttcgatgg catagtccag caggtcggaa
    17821 gtcggcagtg tctggaaatg attgtctttc aaattcagga catccacttc gttctgggaa
    17881 aaccgcctga tatcctcctg tttagcgtgg ggtttgatca gcggtgcgtc gagcgccaca
    17941 atacggggat agtcgttttc cacgcgattg acggctcttg tgcccaggcc gaagacgatc
    18001 cgcagcattc cggctttcgg atccatgcct ttttgccaga cgaacgtgtt ataggaaagg
    18061 cccacaccgg caatgtcggg gaagaaatat ctgttgcgat gtgatccgga aacgcgctgc
    18121 accagcagcg ccatctgttc gtcctgatga gccatgccgc gttgaacacg ataggtcaga
    18181 gcgtcctcat tcatcgtgga tgcgaaaatt ttccttacgg catcctcaaa gtttttatat
    18241 cgtgtttcag gcgtgccctg attcgggcag aaatagcttt cgtattttcc ggcaaaggcg
    18301 ttgccgaagg catcttccag aagcgaactg gaccggacga taatcggcga ctgcccgaaa
    18361 tattccagca tgagctgaaa ctgctctttg atttcgtcgg ggaaaatgcc gtgaagcatc
    18421 ctgcttttaa gctcccgggc gacttcaaaa tatccctcgt cggttttttg cgccatcagc
    18481 agtttccacc agccgttttg caccatatat gaataaaaaa tgtccgagcc gatataaaag
    18541 gaatcgtgca actccagttc ggaatcccag tcgaaggcgg ggtccttgcg gagaatattg
    18601 cgcgccatca gcataccgac cgatttgccg ccgataaacc ccgtgccgat gaggcgtttc
    18661 ttgatttcca gcaaatcttc cagtgaaaag tattccttga ccagcgaaag cattcttttt
    18721 tcacgcccga tcattactct cgaaagagaa tcgaccattt cctgtctatc aaggacggcg
    18781 tcggggtctt caagaagatt actggccttt aagaacaggc ggtcccaata atccagattg
    18841 cgcacgccgc ccgtggtgtt tttgttcgtg agataggaaa gaaaacttgc cgcatccgcg
    18901 ctgtttaata tcgggacaaa tttatcattt tccatgatgt gcggcaggaa catggtcggc
    18961 gtgtagcgtt tccatgcctt aagcgggtgg acgcacaact ggccgttgct gttgtgaata
    19021 tccagcagca cctgcgttgt ttcgcgaatc cgcgctacgg ttttaaaaga gtggctgctg
    19081 cgcagaatgg caaaatacgc gactgtgttt agttcgaaca gataaggaca ggtgatcaca
    19141 aaaaagtttc cgatcatcag atcggtggcc caggccatca acagatcgga caggcagtcg
    19201 aagacataaa acacatcccg tccttcttcc ttgataatgt tgtgcacctg tgtcgaaaac
    19261 gattcaaacc cgttgttggc gttgagcttg tataccttga tttttttgtt tgcctccagc
    19321 agaggcgggt gagcggcaaa gcgcatgtaa accagccgat gcctggaagc aagagaggtt
    19381 ttgacgaacg gctcgacaaa ttttttgtaa tcctcgatgt cgtcaacctg caggacaaca
    19441 ttatccccca tttgaagacc gttgatgatg tcatcgaggc tgctcgttcc tgtgctgacg
    19501 cgtgcatatg gtgtcataaa tctttccctc atccattatt cggaacaaat ttgtaccata
    19561 aataatcgtt ccatttttat ttcttcttgt cctaaacggc gcctcttttc aaaagaataa
    19621 taatttcaag tattttcatc agcacaatga cgtttatcgg tgcatccggc ggtgtttgat
    19681 gaaaaatcag tttctgcttc ctgatagatg aatcaatttt tgtgccagaa tatttttctt
    19741 cttgcatgga acgaactgca ttcgggaggg ggaggtagaa attaaaaatc tttggacggc
    19801 aattttgcgg aagaaacatt tctgaaacgt tttttgagaa gccggattgt ttttaaacgg
    19861 gagaagtgtc aacatttcag gactcaaaaa ccatgattta tacagtattg tatcaattta
    19921 tcagcctcaa aattttcgta ttaataaata ataacaataa cttatcacaa taaaaacatt
    19981 ttggcattgt ccttgccata atacagacga aggataaatt catcaggagg tattgattat
    20041 ggattctgga aatacagcgt gggttctgac agccagcgcc ctggttttgt taatgaccat
    20101 tccggggctg gcattttttt acggaggact ggttcgccgc aagaacgtcc tctccattat
    20161 gatgcaatgt tttgtcattg tttgtgtaat cagtctgcag tggatgctgt tcggttattc
    20221 gctggcattc ggcccggatt ttcacggcat catcggtaaa ctggactgga tggggctggc
    20281 cggagtgggc gcaacgccga atccggatta ttccggcggc attcctcatt acgcctttat
    20341 gatttttcag gccatgttcg cgatcattac gccggcatta atcatcggcg cttacgcgga
    20401 acgcgtgaaa ttttccgcct tcctggtctt taccttatta tgggcgacct ttgtttatga
    20461 tccgctggcc cactgggtct ggggtgtcgg cggctggatg agaacgctgg gcgccctgga
    20521 ttttgccggg ggaattgtcg tgcatgtcag ttcgggaatt tctgctttag tgctggcttt
    20581 aatgctgggc aaaagaatcg gctacaacca taaacccatt cggccgcata atctgccctt
    20641 taccgttctg ggcgccgctc tattgtgggt tggctggttc ggcttcaacg cgggcagcgc
    20701 cctggccgcc gatggcctgg ccgccaatgc ttttgtgacc acacatatcg ccacggctgc
    20761 cgccggcatc acctgggctt tgattgaatg gtggcacaac ggcacgccga ccattttggg
    20821 cgcggcaaca ggggccgtgg ccggtctcgt tgccgtcacg cccgcctgcg gttttgtcaa
    20881 tccgatgaat gccatgttta tcggcatgat cgtcaccgtc gtctgctata tcgcggttgc
    20941 ggtcatcaag ggcaaactcg gctacgatga ctctctggat gccttcggcg tgcacggcgt
    21001 gggcggaacc gtcggaacca tcgccaccgg attgttcgcg gaaaaagttg tcaacgccgc
    21061 cggagccgac ggtctgcttt tcggcaatgc ccatcagctt tacgttcagt ctctgtcgct
    21121 gattgtcacc gtcgcctttg ccgcgatcat gaccttcatc atcttcaagc tcgtggatgc
    21181 gctcatcggc atgcgtgtgg aagagaataa tgaagtcgtt ggtctggatt taacgcaaca
    21241 aagcgaagcc gcttacaccg tgatcgaata ggaggaatgc attatgaaat tagtcatagc
    21301 cattattcag ccgcacaaac tggaagcggt gaaaagagaa ctggaggccg tggacgtcaa
    21361 tctgatgacc gtcaccaatg ttctgggaca gggccggcaa aaaggcgtca cggaaattta
    21421 ccgcggcgcg aaggaagccg gaggcctgct caataaagtc aggctggata tcgcggtcaa
    21481 tgaagatttt gtcgaaccga ccgttaaagc gatcacgaaa ggggcccata ccggctcggt
    21541 cggcgacgga aagatcttta tcgtcgaact tgccgagtgc ataagaatca gcagcggtga
    21601 acgcggtggc ttagccatcg gttgaggcgg ccgctatgag catacgcatc cataaagcca
    21661 aaacaaacgc cctggcccgt tgcgtttccg aatgcgaaaa agcggtgatc atgcacgttc
    21721 tgctcaaaac gaaaggcgat caatcggaag cggcttcact tttgggaatt aaggaaagtt
    21781 ttctggcgga taaaattcat caatatgaaa ttgattgcgc ccgattcgaa ttaaaataat
    21841 tgtaaaggag aatgaaaaag atgaaagaca atttaaaaac tggaataatc atggttttgc
    21901 tgactctgat cctggcattg ctgcccgtga tgagttgggc ggatgaagaa atagttcctg
    21961 ccgatccgaa acagggagat aacgcatcaa cggaagtcaa acctgtggag caagatcagc
    22021 cggaagataa agtgaccggc gaggttgcgg caagcgtttt gagcgcctat atctggcgcg
    22081 gacaggaatt gacccgtcac agtgtggtca ttcaaccctc cgttaccgcc gcttataaag
    22141 gctttgccgc caatgtctgg ggaaatcttg atacaaaacc gtactctgct ctcgatgtgg
    22201 attattcatc caacttcacc gagacggatg ttaccctgtc ctatttgcat aaattcggga
    22261 tcttgcaggc gggcataggt tacatatatt acggcctggc aggagccgtt cagggcggcg
    22321 ccgatcttcc ggattcacag gaagtgtttg taatgcttgg acttgacacg attcttgctc
    22381 cgacgctgac tgtttataaa gagttcgatt actacaatca gtggtatgct acgctgggaa
    22441 tttctcatac ttttgcgttg catgaaaaag ttggtctgaa atttgctgct caagcaagtt
    22501 atttgagaag cgacgatgag gcaacgtatc cggaatttga cagcgcttca ctggccacga
    22561 ccgataaatt taataatttg cacgacggtg ttgtctccgt ttctctgccg attgccgtaa
    22621 ccggccatct gacaatcagt cccgtgctga cctacgtctt tccgctttcc gatgatgcga
    22681 aatacgagat gaaagcccgc ggactgaaag gagacatcga tccctcccat aaggaaggcg
    22741 attttctgta tggcggcatt gtagtgagtt acgcattcta aatttaaatg ccgatgcgtt
    22801 ttggtagatg atctttgaat ggattttgct gatgggtcgt ctaaaggtag gacaacaggt
    22861 tctggccctg tgaatcgagg ttcgagtcct tgcccatcag ccatttgcag caaatttact
    22921 gagatactaa aaatttcaat gacaatcgga gaaaaaaact ttcatgcgcg cgcactactt
    22981 tcagcatgtt cctttcgaag ggctgggcag catcgagtcc tggctgaaaa aatccggttt
    23041 taaaataacc agcaccagat tctatgaatc ggccaaactc cccgatctta aaacgataga
    23101 tcttcttgta gtgatgggcg gtccgatgag cgtcaacgac gaagatcgat tcccctggct
    23161 ggttgccgaa aaacaattca tctgggatgc catcgctaaa agtaaaccgg tgctgggaat
    23221 ctgcctgggc gcgcagctta ttgcca
//
LOCUS       DAOSME010000035        23241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2759_length_23241_cov_22.864660, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000035 DAOSME010000000
VERSION     DAOSME010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23241
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_23241_cov_22.864660"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLG98_06645"
     CDS             <1..460
                     /locus_tag="PLG98_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32969.1"
                     /translation="VAGIYAVGDVTGGMMLAHRAMQQAKFAVERIFGGDDFAYNEDCI
                     PSVVYSHPQIARVGYTQKRAGEEGLSVEVVKSPYSANIIARAELTGQGFVKAVFHQNK
                     LIGATIVGEDAAELIVPLALIVSNQMTKKQLKGWVLPHPTLSEVFALLTG"
     gene            475..825
                     /locus_tag="PLG98_06650"
     CDS             475..825
                     /locus_tag="PLG98_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE32970.1"
                     /translation="MKSINYKTVAPTHFDKDSARGVAGRVVTGKADGASNFCMRVFEI
                     APGGFTPKHSHPWEHEIFIHAGSGEVYGNGQWNPIDPESVIFMPAGEEHQIRNTGKET
                     IVFVCLVPSVAPEL"
     gene            complement(838..2175)
                     /locus_tag="PLG98_06655"
     CDS             complement(838..2175)
                     /locus_tag="PLG98_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE32971.1"
                     /translation="MQKEKNENDIIVIGAGAAGMMAAGRAGELGAKVLLLEKTDDCGK
                     KILVSGKTRCNLTNAAELNHFIAMYGDNGRFLFSAFHHFFRTELLDFFNHHGLATKVE
                     RGGRIFPVSDDAHDVVRIFKKYLSENNVTLKVNTKVTGITMKDDSVYGVKTKDQDYRC
                     KAVVLATGGATWPSTGSTGDGYKIVAAWGHNIVKPKPALVPLIVREQELAQSMQGVSL
                     RNVRATAFQGEADSIDSRMTPDCIYGRGEKKSPRHPVIESRFGEMLFTHFGLGGPIIL
                     LMSLSVVEALEKGLVSVLIDLKPALTREQLHKRLQRDLDNFSKRKISGILKEYLPAKM
                     IEPFIGLTGINNEKLAHQINAAERGKIVEILKALRFNIKASLPLEKAIVTAGGVSLNE
                     IDPRTMASKLISGLFFCGEVMDIDADTGGYNLQAAFSTGYLAGEKAVEYIKAS"
     gene            2550..2837
                     /locus_tag="PLG98_06660"
     CDS             2550..2837
                     /locus_tag="PLG98_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32972.1"
                     /translation="MKKVVLLVVALIFSFSMTVAAVEKAEVPDTSEPVKVADIEKDDI
                     KKGDVKKDKTKKSKKKNVKKSKDKKSKKSSKKDEKIVEDAESEVPAAPAAK"
     gene            3190..4740
                     /locus_tag="PLG98_06665"
     CDS             3190..4740
                     /locus_tag="PLG98_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32973.1"
                     /translation="MVKTILKKITAFNNRLTVRSKLLVLIVSGLALTMGVWGIIQLAA
                     LERILVEQQAKRLKDVAETVSTFYQDFPTKKGLKALESALHDHLQADGRLARIDIFDI
                     GQKHFDYVAGASRDPYQWADNIVMSVMKKNKLHYMKIMTENGPALGLLYPVAGNTKSV
                     RIVIGIISFSRANEEIVHRAGRLLIISSAGLLIFILVVLGLSYRWIIGKPLKLIIHAI
                     DEFQEGQYDNRIPIVHRDEWGQISRHFNMMADEIQDVMERNQELTKHLEKRVHEETIK
                     VFQLQKQVTDLKQLTTLGQLTANLAHDLGTPLHSIAGLAKLLLEKGGLPENVEHKLNL
                     IVQQTLRLDAVIQNVRKAIRLPDPSFEALPVEQIFHEMLSLMEPFLQKNNVESVVTIT
                     NPVSPLYVDRYRIQTALMNVMQNALEAMPEGGTITMSAHEVPCENFVAITVSDTGYGM
                     TPDVVENVCEPFYSTKTSKGLRGLGMAIVRDVMKIHRGQMEIKSSRGQGTMVILCFPK
                     LKDADHFH"
     gene            complement(4789..6111)
                     /locus_tag="PLG98_06670"
     CDS             complement(4789..6111)
                     /locus_tag="PLG98_06670"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE32974.1"
                     /translation="MKKRILVVDDDAVACEFLEETLKRDGYDVTLCSSAWEALRQNIA
                     SYNLVMSDIRMSGMDGLQLLEELQKQSPDLPVILMTAYGSLETTMAAVNKGAWDYISK
                     PFSPEDCRTLVRKVLELRKLRHKGMKLQPAKVEESKFIGSSAMMVDFYKQIARIADSS
                     ASVLIEGESGTGKELTARSLHNMSSRREKPFIIVHCGAIPDNLLESELFGYNKGAYTG
                     ASHQHTGLLETAEGGTVFLDEITEMSTALQGKFLRFMQNGELRKIGDNAVRNVEVRVV
                     AAANKKIDDEVKKENFRLDLLYRFIVRLRIPPLREHKEDIPLLTEALLKKLGDTNVSV
                     SPDAMEMFMAYDWPGNVRELENVLQQIMLLSPFDEILPENLPERFHSSTDRKDILNGP
                     GLSPLEEAEKEKILQTLKETSWNQSQAAKRLDIDRKTLRTKIRRYSLS"
     gene            complement(6120..6596)
                     /locus_tag="PLG98_06675"
     CDS             complement(6120..6596)
                     /locus_tag="PLG98_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOE32975.1"
                     /translation="MTGQHNTIEIGSEMPEYISLPITRTHLVRYAGASGDFNPLHHDQ
                     TFANTIGLQNVIAHGMFIMAIAGEAITSWIGNKNIKKFHSRFLAKTEPVDWDNFENTK
                     DRATIVVKGKVIRLFVENGEKLFECKIVAENLLGSKKLSGSFIAVVTGEPPRDVNE"
     gene            complement(6601..7065)
                     /locus_tag="PLG98_06680"
     CDS             complement(6601..7065)
                     /locus_tag="PLG98_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOE32976.1"
                     /translation="MADQSKIGMEFPASTFIIEKDKIIEFAIAVSQKEMKNQIHPVYH
                     DEKAAKAAGYEGIPIPPTFLTSSFFWTGDGLTGMVTALGINLKRLLHREEEYEYFCPI
                     HAGDIITRKMKVADIIKKGKGARTLDMTILEVEYINQRNELVAKSRTTLTEI"
     gene            complement(7264..8460)
                     /locus_tag="PLG98_06685"
     CDS             complement(7264..8460)
                     /locus_tag="PLG98_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE32977.1"
                     /translation="MINLEMTQKMMDVKSGMNNMASSMLRPVSRHYDEHEHEYPKELD
                     VFRSIKIMSRPKKKKEGGETKPDKPGAKKLGANLSMVIVIEEMCWGDLGLLLSLPNAG
                     LGNAAIAAVATDEQMERFGEKWASMAITEPGSGSDSGSITARATLEGDEWVLNGEKIF
                     VTCAERAEVAVIWATVDRSAGKAGIKSFVVEKGRPGFKLEKLEHKLGVRASDTGTYVL
                     DDCRIPKDNILGSAEVKQSTEGFKGAMKTFDNTRPLVASMALGVTRASLDFTQEKMEA
                     SGITLDYEKNPNNLSAMEKEFYLMEAHLDAMRLLTWRAAWMMDNGEFNNLEASMSKAK
                     AGRYGTFICQKCCELLGPLGYSCENLAEKWMRDVKITDIFEGTGQIQHLIVARNVLGY
                     SSKQLK"
     gene            complement(8472..9581)
                     /locus_tag="PLG98_06690"
     CDS             complement(8472..9581)
                     /locus_tag="PLG98_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE32978.1"
                     /translation="MEDRDFGFGMSEEQSSMKDTVAKLVKSMVSGNPAHDMDEARKLP
                     REIIDKVWELGATVSTVPEEYGGYGMEYSPIMNAIFVEELAAGDMALAIASTLPATFI
                     YTILQMGTDEQKKKYLPLYCTPTYQACTVAINEPLFRFDAVSLETKAVKNGSKYIING
                     KKCFVPMAKDSGHMLVAADIDGKMDLFIVEGNNPGLKIGEREKNLGMYALESFPVVLE
                     NCEVPDDDRVGGDNGCDYSRFLQKTRIAMSAIGTGVSRASYEYAREYAKERIQFGEPI
                     ASRQAIAFMIAEMAYEVDAMRLMTWKAASALEAGRDAKKEAYLAKLYAGEMTMKLTDF
                     GVQILGGHGYIRENPVERYYRNGRGIAILEGMATV"
     gene            complement(9774..10343)
                     /locus_tag="PLG98_06695"
     CDS             complement(9774..10343)
                     /locus_tag="PLG98_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOE32979.1"
                     /translation="MAKFKAPSGVSVNDFFEKLVPEQFKEVIAGANLSSLTGKEVNLQ
                     FNVNDQQYCLKIKDGTNLDVVKGSVDKPLLTLSLSEQVFLDAVTGKAEGLIDRFTDPV
                     EIADPMRLKNLMDTKGTLNLKLKQDGNEIPVTMIFNGEEAPKVDISLDLQDWIAMQNK
                     QTTGQNLFMNGKLKFAGDMTLLMKLQTLI"
     gene            10612..11301
                     /locus_tag="PLG98_06700"
     CDS             10612..11301
                     /locus_tag="PLG98_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE32980.1"
                     /translation="MNDIEKKLKSSLQKLSKPSGSRSSSNEGNVRERILKAARDVFTV
                     YPFKEAGTRMIAKQAGVEHPMIHYYFGSKENLFYAMAEDLYHETLSVQEAWLDGMENM
                     SLKEGFELFLDRMLDYTMQKPDALRIAALNVVNIGNLEEIPGYRFIIMSMASMRRIIE
                     EKLSLKGSNREIEMFIYCFYSLMISLIGARSCQAQILNMNPLEEKYRTWVKDASMTLF
                     YPWFKKILVGR"
     gene            11460..11597
                     /gene="rpmH"
                     /locus_tag="PLG98_06705"
     CDS             11460..11597
                     /gene="rpmH"
                     /locus_tag="PLG98_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOE32981.1"
                     /translation="MKKTLTNKTNTKRKKTHGFLVRMASKAGREVLNRRRAKGRKRIA
                     V"
     gene            11996..12223
                     /gene="yidD"
                     /locus_tag="PLG98_06710"
     CDS             11996..12223
                     /gene="yidD"
                     /locus_tag="PLG98_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOE32982.1"
                     /translation="MLRKIFINFFVSFIHLYQACLSPFFFAPCCRFYPSCSQYAIEAI
                     KLYGPAKGSWMGIKRILRCHPLNPGGYDPVK"
     gene            12242..13957
                     /gene="yidC"
                     /locus_tag="PLG98_06715"
     CDS             12242..13957
                     /gene="yidC"
                     /locus_tag="PLG98_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOE32983.1"
                     /translation="MDKRTILAVVLSLAVLFAYQIFFAKPPVPPETVPVRESHQTAQK
                     PTPIKENSVVKQQAVSPDASTTKQKVKNVLPAQEESSRDIKVETANYTAVFSTKGAAL
                     KSFQLKGYQKECTKCVGDIWPAIKNFVTGKKQPAGEKNNDLIEVVSLQPDMPYPLTLT
                     FPESSGEIAADSVYKTDTAELNMVKNSKKQRLVFSRVFNDVRVEKIYIFNPDNFSITL
                     EVNISNLTDVPITKIPYLNWYDYVDPKEKVDGYSKEGPVAYVSKSIERWKSADVSKEQ
                     TIGPDVSWGGVERKYFMAVIIPQDPSLTSFNMSRDDHVIAVNLKGAKNIIPPEQSGSF
                     KYNLFIGPKDYAMLKKLGLSLEEAIEFDSVIPGLKWLSIGLLLFIKFIHQFVGNYGVA
                     IIILTILIKVLFWPLGNISYKSMKEMQKIQPQITALREKYKNDQAKLGQETMALYKTH
                     KVNPFGGCLPILIQIPVFIGLYNTLLYAIELRHSPFFFWIQDLSAKDPYYITPIVMGA
                     TQFIQQKMTPTMGDPMQAKIMLAMPIIFTFIFLNFPSGLVIYWLVNNIISIGQQIYIN
                     KKLTD"
     gene            13991..14875
                     /gene="jag"
                     /locus_tag="PLG98_06720"
     CDS             13991..14875
                     /gene="jag"
                     /locus_tag="PLG98_06720"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOE32984.1"
                     /translation="MSSEFIEIEEKTIDEAIEKACQTFGVPREKLNIEIISGESGGFL
                     GLFSKKAKIRASLLALDIDFSFSDKPKEKTDKPREKNEPRSKADHYREKSEAKVLPKR
                     KPEKEVKEISEEKSPVKKEREAFIPNSPLSLKAQELLEGILQRISPEYKANVQETQEK
                     IILGIEGDESGLLIGKRGQNLDAFQYIINKAINKIDSEHKMILIDSGEYRKRREDFLL
                     DLAEKIKKKVKKTKKPVSIANMNAHDRRIIHLVMQEDESLVTQSRGEGKYRKIIILPA
                     KNGSSRRTKEKKKDINQS"
     gene            14894..16273
                     /gene="mnmE"
                     /locus_tag="PLG98_06725"
     CDS             14894..16273
                     /gene="mnmE"
                     /locus_tag="PLG98_06725"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOE32985.1"
                     /translation="MLRSDTIAAAATPFGMSGIAVIRVSGPEALAVARRIFRPSNRPC
                     DWKSHHLYHGDIVCADGETVLDEILIAFMRKPRSFTGEDVVEISCHGNPVIIQSILDQ
                     LQKLGCRLAGPGEFSQRAFLNNRFDLSQAEALASMIGAQSEKAYAVGLAQLKGSLSKE
                     IETIRGLVIEALALMEASIDFAEDTAEAELVQIPPQINQAMERMQRLLTSYRFARAYS
                     EGVHVVITGKPNVGKSSLLNALAGKKKAIVTDIPGTTRDLIADTIHIGGIPVHLTDTA
                     GIREPQNVIEQEGIALVWKKLAEADIVIIMLDVSKPLTEEDKITIDKNTSEKIIVAVN
                     KIDLPRSWETKSIANLFPPHTKFLEISAKSGSGLEELKRTILDLSVDGETESTSGAIL
                     TNLRHKHAVEQSLKNIQNAKESVTAGLSAEFIAFDLREALDHLDEITGKKINDEILDR
                     IFSSFCIGK"
     gene            16426..18096
                     /locus_tag="PLG98_06730"
     CDS             16426..18096
                     /locus_tag="PLG98_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE32986.1"
                     /translation="MHGMLTDIGISVLAATALGFVFQLVRQPVILGYLVAGALIGPAM
                     GFNLVSEAASIEVISEIGLILLLFIIGLELNPSKLLASGRQLIYAGIGQFIFCVLIGL
                     GFFLLLGYELSRNNIDALYLALFCALSSTAIVVKLLYDKFELDTFPGRITLGILIFQD
                     LWAILILALQPHFTNPQFLLVFIALGKSVALLAIGFLLSKYLLSWIFKKISQTPEMVV
                     AMSMAWCALMAGIGSWIGLSMEMGALIAGVAISTFPYSMHVTAKVLPLRDFFLTLFFL
                     SIGMKIPFPDAAILGMALIIVLFVIVSRFLTIYPLLTLAGSGRRTSFIASLNLAQISE
                     FSLVVAALGVHYGHIDQRMMSLIIYAMAMTSVLSSYFIKGNHAAYLVFNRFLEKRGFP
                     ARTKENHDEEEKNVYPIVILGFHRGAREFIGRLETETPDLLKKLLVIDFNLENLKELS
                     NKNIKGIFGDIGSMDTLEHAHITAAQIIFSTIPDMLLRGTSNKALVTSCRALAPSAYI
                     VATADSADQIDVLRKSGADEVLLPYVLIGEKLTRFVEQTCLTAESCKL"
     gene            18132..19052
                     /locus_tag="PLG98_06735"
     CDS             18132..19052
                     /locus_tag="PLG98_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE32987.1"
                     /translation="MGKKVGLVLGGGGVRGFSHIGVLKVLEEEGIDIDLIVGTSAGAL
                     IGGAYASGQAPDEIESKIDAYLQSPEFDDSRMKSIGLSFTQEEKNILKKAQQFIMNRV
                     LFVQAFFKPSILPSHDFLSLMNYFLPDLDIKETRIPLHVVSTDLISGKKIVFSEGSLR
                     KVVLASCAVPGAVEPVRYGDWLLADGGITSLVPVHAAREAGADVVIAVTVDRDLQTNL
                     RIETAKDVVFRAGEITPSVLEAAELADADVIIRPQVGNLHWMDFKRAVDLVKAGESAA
                     RDSLKSIHASLPIYRRITRLAGKLFKQSRL"
     gene            19302..19778
                     /gene="rpsF"
                     /locus_tag="PLG98_06740"
     CDS             19302..19778
                     /gene="rpsF"
                     /locus_tag="PLG98_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOE32988.1"
                     /translation="MQRYEVVAIVLADLIEDEINALIERSQTIITDRKGVIAKVDKWG
                     KRHLAYEIKKQRDGYYFLIDFAGNGSIVNEIERNYKIDDRVIKFMTVKKEGATTAEGI
                     QQEAAAAEAKRVQAKVEAEAAAAEGKQINQIEKAPRESRPRVRKDHNKEETQTKGE"
     gene            19788..20057
                     /gene="rpsR"
                     /locus_tag="PLG98_06745"
     CDS             19788..20057
                     /gene="rpsR"
                     /locus_tag="PLG98_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOE32989.1"
                     /translation="METQNGEKQGRYPQKKFFHRKKFCRFCSDSNIKIDYKNTAILNN
                     FVTERGKIMPRRITGNCASHQRELALAIKRARQIAIMPYVTADGR"
     gene            20228..21205
                     /locus_tag="PLG98_06750"
     CDS             20228..21205
                     /locus_tag="PLG98_06750"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOE32990.1"
                     /translation="MSSFSFFKKSGAPASGNSFSGLLSAVGLFAFAAVFVPLVGPVFL
                     FLLPMIAFVNGATNGIFKTALCLLFSLAVLMLLSVGLNLDVPAVAIFTMGMAGLLMAH
                     MAAENYSIEKIVTGPVLLIFGAIGFYFVYDAVGLGVNPWLLVKNFITVTISETIKFYS
                     QMPLKPDDISMIKDNENNIINSLIHIFPSLMVILSVFIIWANLLLGKTYLRRFGIVYA
                     QMAILARWKVTDWAIWIFIISGLLLFIPQKNFNFIGLNVFLIVCLVYLLQGLAIVSFL
                     FQNKNVPFLIRYIFYFLIAVQQILMIPIAVIGLFDVWFDFRKRFQKNHL"
     gene            21250..21702
                     /gene="rplI"
                     /locus_tag="PLG98_06755"
     CDS             21250..21702
                     /gene="rplI"
                     /locus_tag="PLG98_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOE32991.1"
                     /translation="MKVILKQSVRSLGKAGTVVKVNDGYARNFLIPKGLAIEADEKNI
                     RLFEHEKNNILKKAAKEHKNAQDLADVLSRETITIARKVGDQDKLFGSVSAKDIEAAL
                     KEKGYDINRKMIMHEHGEHIKELGEFAVKIKLAHDVEAQITLKVVGES"
     gene            21702..23066
                     /gene="dnaB"
                     /locus_tag="PLG98_06760"
     CDS             21702..23066
                     /gene="dnaB"
                     /locus_tag="PLG98_06760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOE32992.1"
                     /translation="MKEKDPSQYKLPPQNLEAEQSVLGSILLENSATNRVFEILSERD
                     FYSEAHRKIFIVIKDLSEKNEPVDLITLSNALKDKSLLDSVGGTTYLASLVDNVPSAA
                     NAANYAKIVKEKAILRGLITSATEIIDNCYETGSDVDTILDKAEHSIFEISENKVRQD
                     YFPIRDIIKDSFRSIEDLYSRQQLITGVPTGFGKIDDLTAGLQKSELIIIAGRPSMGK
                     TAFALNIALHAAMEAQIPTAVFSLEMSKEQLAFRLLSSEARVDAQRLRKGFLGETDWP
                     KLTTAAGRLSDAPLYIDDTPAITVLEMKAKSRRLKADKGLGLVVVDYIQLMRSSYSHD
                     SREREISDISRSLKALAKELNVPVVALSQLNRQVESRTNRRPQMADLRESGAIEQDAD
                     VIAFIYRDEVYNKSEDNIDKGIAEIIIAKQRNGPTDTVKLAFVDKYTSFENLSRVELP
                     EGQH"
BASE COUNT         6128 a         5343 c         5527 g         6243 t
ORIGIN      
        1 tgttgccggc atttacgcag tgggcgatgt taccggcggg atgatgctgg cgcaccgggc
       61 gatgcagcag gcaaaattcg ctgtggaacg tattttcgga ggagatgact ttgcctataa
      121 cgaagattgc atcccttcgg tcgtttacag ccatccgcag attgcgcggg tgggttatac
      181 ccaaaaacgc gctggggaag aaggcctgtc cgttgaagtc gtcaaatctc catacagcgc
      241 caatattatt gcccgcgcag aattgacggg gcagggattt gtcaaggcag tttttcatca
      301 gaataaactg attggcgcga ccattgtcgg tgaagacgcg gcggaactga ttgttccgct
      361 tgcactgata gtatccaatc aaatgacgaa aaagcagttg aaaggttggg tgctgcccca
      421 tccgacattg agcgaagtgt ttgcattatt aaccggttga caagggagtc ccttatgaag
      481 tcgatcaatt ataaaacggt cgcaccaact cattttgaca aggattcggc caggggcgtt
      541 gccggacgag tggttacggg caaggctgac ggcgcgagca atttttgtat gcgggttttc
      601 gaaatcgctc cgggaggatt tacgccgaaa cattctcatc catgggaaca tgagattttt
      661 attcatgccg gttccggcga ggtttacggc aacggccagt ggaatccgat cgatccggag
      721 agtgtcattt tcatgccggc cggtgaagaa catcagatca gaaacaccgg gaaagaaacc
      781 attgtgtttg tttgtctggt tccgtccgtt gcgcctgaat tgtaatgccg gagatattta
      841 agatgccttt atgtattcga cggctttttc accggccagg taaccggtag agaaagcggc
      901 ctgcaaattg tacccgccgg tatcggcgtc aatatccatc acctcgccgc aaaagaaaag
      961 tccgctgatc aatttggaag ccatcgtgcg cggatctatt tcgtttaacg aaacaccgcc
     1021 agccgtaacg attgcctttt ccagcggcag agacgcctta atgttgaaac gcagcgcttt
     1081 caaaatctca acaatctttc ctctttccgc ggcgtttatc tgatgcgcca gtttttcatt
     1141 gttgattccg gtaagcccaa taaatggttc aatcatcttg gcgggcagat attctttcag
     1201 gatgccggaa atttttcttt tgctgaaatt gtccagatcc cgttgcagcc gtttgtgaag
     1261 ttgttcgcgg gtcagcgccg gttttaaatc gatcaatacg gaaaccagac ctttctccag
     1321 ggcttcaacg acggacaggc tcattaataa aatgatcggg ccgcccagtc cgaagtgggt
     1381 gaagagcatt tccccgaaac ggctttcaat aacgggatgc cggggcgatt ttttttctcc
     1441 ccgtccgtaa atgcaatcgg gcgtcattcg ggaatcgatg gaatccgctt cgccctgaaa
     1501 agccgtggcg cgtacgttgc gcaggctcac tccctgcatg gattgagcaa gctcctgttc
     1561 ccgtacaatc agcggcacca gcgccggttt cggtttgacg atgttgtgtc cccacgcagc
     1621 gactattttg taaccgtcgc cggtggaacc ggtggaaggc caggtggccc cgccggtggc
     1681 cagaacgaca gctttgcagc gataatcttg gtcctttgtc ttcacgccgt aaacggaatc
     1741 gtccttcatg gtgatgccgg ttactttagt gttcactttt agagttacgt tattttcgga
     1801 aaggtatttt ttgaagatac gcacgacatc atgagcatca tcggacacag gaaatatccg
     1861 tccaccgcgt tccaccttgg tcgccagacc gtgatgattg aaaaaatcaa gcagttccgt
     1921 acggaaaaaa tgatgaaagg cgctgaaaag aaaacggccg ttatcgccat acatggcaat
     1981 aaaatggttc agttcggctg cattggtcaa gttgcagcgc gtcttgccgc tgaccagaat
     2041 ttttttgccg cagtcatcgg ttttttcaag gagtaatact tttgcaccca attcaccggc
     2101 acgaccggcc gccatcattc cggcagctcc tgcgccaatc acaataatat cgttttcgtt
     2161 tttttctttt tgcataaaga tcattcgatt ttttaatggg gtaacatgaa agagtagaaa
     2221 cggtcaataa ttctatgctg aggaataaat cctctccgat ggggaattat tccccgcatt
     2281 tctttaataa aaaacattca tggcgtgact taataaattt aacttttgta ttttcagata
     2341 catgcaacaa ataaagactg tctttgtact ggcatgtaaa ttgctttaat gcgtgaacga
     2401 caaacgtatc cacctaactt ttaaagatag aaacaatatt ccaacttaaa taaatgtgtc
     2461 ctggcgacat gattattgaa cttttgtttg tgtccctcaa tgagggtgat gttgcaataa
     2521 aataaaaatt gatcaaggag gaatttcaaa tgaaaaaagt tgtattgttg gttgttgcat
     2581 taatcttctc attttccatg acagttgctg ccgttgagaa agcagaggta ccggacacat
     2641 cagagccagt taaggttgct gatattgaaa aagatgacat caagaaaggt gatgtaaaaa
     2701 aagacaaaac caagaaatca aagaaaaaaa acgtgaagaa gtcaaaggat aaaaagtcta
     2761 agaaatcatc aaagaaagat gaaaaaatcg ttgaagatgc ggaatccgaa gtacccgctg
     2821 ctcccgccgc aaaataagtt agtaaaatct ttttctggtt ctaaagaagg gataggttgt
     2881 gatctatccc ttctttttag tgaagacttt ttagaaatga gtactgcgaa atttgcagag
     2941 caagtcgaac tgttcaaaag cctttggctt ttggaaacta ttccctgaat gaatagactc
     3001 gggttttgaa tcaaactgat agttggccaa tttgaacttt aggggatctt gaggtgaacc
     3061 atatcaggtt tatagagcta tggtaaaaaa aatcccgacg aaacgggatg aagctctttc
     3121 ctgtcgtttt caagtttctg tgttcaagtt ttgtagataa ttgtttttgt ctttgatata
     3181 ccctcgcata tggttaagac aattctgaaa aagatcaccg ctttcaacaa caggttgacg
     3241 gtaagatcaa agttgcttgt tcttattgta tctggtcttg cattgaccat gggtgtttgg
     3301 ggaattattc agctcgccgc tctggaaaga atattagttg aacagcaggc gaaaagattg
     3361 aaagatgtcg ctgaaacagt cagtactttt taccaggatt ttccgaccaa aaaaggactg
     3421 aaggcccttg aatccgctct tcatgatcat ttacaggctg acggtcgttt ggccaggatt
     3481 gacatcttcg atatcggtca aaaacatttt gactatgtag ccggagcgag tcgtgatccg
     3541 tatcaatggg cggataatat tgtgatgtca gttatgaaga aaaataagct gcattatatg
     3601 aaaataatga cggaaaacgg accggcgttg ggactgcttt atccggttgc tggaaacacg
     3661 aaaagtgtgc gcattgtaat tggtatcatc agtttttcgc gggccaatga agaaattgta
     3721 catcgggctg gccggctttt aattatcagc agcgccggcc tattaatttt cattcttgta
     3781 gttcttggcc tgagttatcg ctggatcatt ggcaaaccat taaagttaat tatccacgct
     3841 atcgatgaat ttcaggaagg tcaatatgat aatcgtatcc ccattgttca tcgagacgaa
     3901 tggggacaga tatccagaca ctttaatatg atggctgatg agatccagga cgtcatggaa
     3961 agaaatcagg aactcaccaa acatctggaa aaacgggttc atgaagaaac catcaaggtg
     4021 ttccaactgc agaaacaggt tacggatttg aagcaattaa ccacgttggg acaactgacg
     4081 gccaatctgg cgcatgatct ggggacgccc cttcattcca tcgctggatt ggcgaaactg
     4141 cttttggaaa agggcggctt gccggaaaac gtcgaacata aactgaattt gattgtccag
     4201 cagacgctca gactggatgc cgtgattcag aacgtgcgca aggccattcg gctgcccgat
     4261 cccagctttg aagcactgcc tgtggaacag atattccatg aaatgctttc cctcatggag
     4321 cctttcttgc aaaaaaacaa cgttgaatcc gttgtcacta tcacgaatcc ggtgtctcct
     4381 ctgtatgtgg accgctatcg cattcagaca gctctcatga atgttatgca gaatgctctg
     4441 gaggccatgc cggagggagg aacgatcacc atgtcggcac atgaggttcc atgcgaaaac
     4501 tttgtggcca ttaccgtcag cgatacaggt tacggaatga cgccggatgt tgtggaaaat
     4561 gtctgtgagc ctttctacag cacgaaaacc agcaaaggtt tgcgcggcct cggcatggct
     4621 atcgtgcgtg acgtcatgaa gatccaccgg ggtcagatgg aaataaaaag ttctcgcggc
     4681 cagggaacaa tggttattct atgtttcccc aaattgaagg atgcagatca ttttcattaa
     4741 aaaaccgtcc tggcgttgaa atgatttttc cccgctgaag tgcttttcct aagacagact
     4801 gtaacgacgt atctttgtcc ggagagtctt tctgtcaatg tccagccgtt ttgccgcctg
     4861 ggactgattc caggacgttt ccttcaacgt ttgcagtatt ttttcttttt ccgcttcttc
     4921 gagaggggat aatccgggac cgtttagtat gtctttcctg tctgtcgaac tatgaaaacg
     4981 ttccggcagg ttctcgggga ggatttcatc aaaaggcgaa agaagcataa tttgctgaag
     5041 gacattttcc aactcccgaa cattgcccgg ccaatcgtaa gccatgaaca tctccattgc
     5101 atccggggaa acgcttacgt ttgtatctcc caactttttc agcaaggctt ccgtcaaaag
     5161 cggaatgtct tctttatgtt ctcttaaagg aggaatgcgc aaccgcacaa taaatcggta
     5221 taataaatcg aggcgaaaat tctctttttt cacctcgtca tctatttttt tattggcagc
     5281 ggcgacaaca cgcacctcga cattgcgaac ggcattgtcg ccgatttttc gaagttctcc
     5341 gttttgcatg aaacgcagaa atttaccctg caacgctgtg gacatttccg taatttcatc
     5401 cagaaatacc gtgccgcctt cggcagtctc cagaagcccg gtgtgctgat ggctggcgcc
     5461 tgtgtatgcg cctttattgt atccgaaaag ttccgattcc agcagattat cgggaatggc
     5521 tccgcaatga acgatgatga agggcttttc gcggcgggag gacatattat gcaacgagcg
     5581 tgcggtcaat tcctttcctg tgccgctttc tccttcgatc aggacgctgg cggaggaatc
     5641 ggcaatgcgg gcgatttgtt tatagaagtc gaccatcatg gccgacgaac cgatgaattt
     5701 tgactcttcg acctttgcgg gctgcagttt catccccttg tgccgaagtt ttcttaactc
     5761 cagcacttta cggacaagcg tgcggcagtc ttcgggagaa aatggcttgc tgatatagtc
     5821 ccatgctccc ttgttgacag cggccattgt ggtttccaga gagccgtaag cggtcatcag
     5881 tatcaccggc agatccggcg attgtttctg gagttcttcc agcaattgca gtccgtccat
     5941 accggacatg cgaatatcgg acatgaccaa attataggag gcaatatttt gccggagagc
     6001 ttcccaggcg gaactgcaga gggtgacgtc gtaaccgtca cgtttcaacg tttcttcgag
     6061 aaattcacac gccacagcat catcatcaac aacgagaata cgtttcttca taaaacctct
     6121 tattcgttca catcgcgagg aggttctccg gtcacgactg cgatgaacga tcccgacagt
     6181 tttttgctgc ccagcagatt ctcggcgaca attttacatt cgaataattt ttcgccgttt
     6241 tcaacaaata gtctgatcac ttttcctttt accacgatgg ttgccctgtc ttttgtattt
     6301 tcaaaattat cccagtcaac gggttctgtc ttagccaaaa aacgggagtg gaattttttt
     6361 atatttttgt tcccgatcca ggacgtgatg gcttcccctg cgatggccat gataaacatg
     6421 ccgtgggcaa tgacattctg taaaccgatg gtgttggcaa atgtctgatc atgatgtagc
     6481 ggattgaaat ccccggatgc tcctgcatag cgcaccagat gggttctggt aatcggaaga
     6541 gaaatgtatt ccggcatctc gctcccgatt tcaattgtat tatgttgccc ggtcatcttt
     6601 ctatatttcc gttaacgttg tgcgggattt tgccaccaac tcattgcgct gattgatata
     6661 ttcaacttcc agaatagtca tatcaagagt tctagcccct ttcccctttt taatgatatc
     6721 ggcaactttc attttccggg tgatgatatc acccgcatgg atagggcaaa aatattcgta
     6781 ctcttcttcc ctgtgcagaa gtctttttaa attgattccc agggcggtca ccatgcctgt
     6841 cagtccgtcg cctgtccaga aaaaagaact cgtcaggaaa gtgggaggta tgggaatgcc
     6901 ttcataaccg gctgctttcg cggccttttc atcatgataa acaggatgaa tctgattctt
     6961 catttctttt tgagaaacag cgatggcaaa ttcaataatt ttgtctttct caatgatgaa
     7021 agtggatgcg ggaaattcca ttcctatttt ggattgatct gccataaata cccctcaata
     7081 tagcatatca tatccgtttc aatatcgtac agtccagtga aaaattataa gaaaatgagt
     7141 gccctggtta aaaagaaggg gtgttttttt aatcttcctc acggggaaga gtggttgaaa
     7201 aattgaaaaa tgcctgcgca agtacaacgc gcaggcattt ttcttaagca aggagaaaaa
     7261 attttatttc agttgtttgc tggaatatcc gagaacattt ctggccacaa ttagatgctg
     7321 aatttgaccg gtgccttcga agatatcggt aattttgaca tccctcatcc atttttcagc
     7381 gaggttttcg caggagtagc ccaaaggtcc aagcagctcg cagcatttct gacagatgaa
     7441 cgtgccgtaa cgccccgctt tagccttgga catggacgct tccagattgt tgaattcacc
     7501 gttatccatc atccaggcgg cacgccaggt gagcagcctc atggcgtcca ggtgggcctc
     7561 catcaggtaa aactcttttt ccatcgcact cagattattc ggattctttt cataatcaag
     7621 cgtgatgccg cttgcttcca ttttttcttg tgtaaaatcc aaagatgccc gggttacgcc
     7681 aagcgccatg gacgcaacca gagggcgggt gttatcgaag gttttcatag cgcctttgaa
     7741 gccttcggtt gactgtttga cttccgcgct tcccagaatg ttgtccttgg ggatgcggca
     7801 atcatcgaga acgtaagtgc cagtgtccga ggcgcgaaca ccgagtttat gctccagttt
     7861 ttccagtttg aatccggggc gtcctttttc caccacgaat gattttatcc cggctttgcc
     7921 cgcgcttctg tccaccgttg cccagatgac cgccacttca gcgcgttcag cgcatgtgac
     7981 aaatattttt tcgccgttga gcacccactc gtcaccctcc aaggtggccc tggccgtgat
     8041 ggatccggaa tcggaaccgg aaccgggttc ggtaatagcc atggatgccc atttttcgcc
     8101 gaacctttcc atctgctcgt cggttgcaac cgccgcaatc gctgcgtttc ccaatcccgc
     8161 gttcggcagt gacaacaaca gtcccaggtc tccccagcac atctcttcga tgacgatgac
     8221 catcgaaaga ttggccccca gttttttggc gcctggtttg tccggttttg tttctccgcc
     8281 ctcttttttc tttttcggac ggctcatgat ttttattgaa cggaatacgt ccagttcttt
     8341 agggtattca tgttcgtgtt catcgtaatg tcttgaaacg ggccgaagca tgcttgacgc
     8401 catgttgttc atgccgcttt ttacatccat catcttttgt gtcatttcga gattaatcat
     8461 cgtcttacct cctataccgt ggccatgcct tcaagaatgg cgatgcctct gccgttgcga
     8521 tagtatcgtt ccaccggatt ttcccgaatg taaccgtgtc cgcccagaat ctgcacaccg
     8581 aaatcggtca gtttcatggt catttcgccg gcgtagagtt ttgccagata cgcctctttc
     8641 tttgcgtcgc ggccggcttc aagagccgat gcggctttcc aggtcatcaa cctcatggcg
     8701 tccacctcat aggccatttc cgcaatcata aaagcgatgg cctgacggct ggcaatcggt
     8761 tcaccgaact ggatacgttc tttggcatat tcccgcgcgt attcgtacga cgcgcgcgaa
     8821 acgccggtcc cgatagccga catggcgata cgggttttct gcaggaatct gctgtaatca
     8881 catccgttat ctccgccgac acggtcatca tcagggactt cacaattttc cagcacgacc
     8941 ggaaaacttt ccagagcata catccccaga tttttttccc gctcgccgat tttgagaccc
     9001 ggattgttcc cttccacaat aaacaaatcc atcttcccgt cgatgtcggc tgccaccagc
     9061 atgtggccgg aatccttggc catcgggaca aaacactttt tgccgttgat gatatatttg
     9121 ctgccgtttt taacggcttt tgtttccagt gaaacggcgt cgaatctgaa cagtggctca
     9181 ttgatcgcta cggtacaggc ttgatatgtc ggagtgcagt acaacggcaa atacttcttt
     9241 ttttgctcat cggtgcccat ctgcaaaatg gtgtagatga aggttgcggg cagggttgac
     9301 gcgatcgcca gcgccatgtc gcctgcggca agttcctcga caaaaatggc attcataatg
     9361 ggagaatatt ccatgccgta tccgccgtat tcttccggta cagtggacac cgttgctccc
     9421 agttcccaaa ccttgtctat gatctcccgg ggaagctttc tggcttcatc catatcatgg
     9481 gcgggattcc ccgaaaccat actcttgacc aacttcgcaa cagtatcttt catactgctt
     9541 tgttcttcag acatgccgaa cccaaaatct ctgtcttcca taatcatctc cttgaatgcg
     9601 gtcatggggc ttgaacgtta caagattatc ctgaaaaaat tgaccgactg gttttcataa
     9661 aaaatagcca ggggtgtgtt atcccaatcc ccccaacggt ttttcgacac acccccggca
     9721 atggtgttta acgcaacagg ttgatccgta atgagtaact tcttctcctc gttttagata
     9781 agggtttgca gtttcattag aagagtcatg tcgccggcga atttcaactt gccgttcatg
     9841 aaaagattct gtcctgtggt ttgcttattc tgcatggcaa tccaatcctg caaatcaaga
     9901 ctgatatcaa cttttggcgc ctcttccccg ttaaaaatca tggtcacggg aatctcattt
     9961 ccatcctgtt tcagtttcag attcaaagtg cctttggtat ccatcagatt tttaagcctc
    10021 atgggatcgg caatttccac aggatccgtg aagcggtcaa tcaaaccttc tgcctttccg
    10081 gttacagcat caagaaacac ctgttctgaa agcgataacg tgagcagagg tttgtctacg
    10141 cttcccttga cgacatccag attcgtgccg tctttaattt tcaggcaata ttgctgatcg
    10201 ttgacattga actgaagatt gacttctttg cccgttaaag atgacagatt cgctccggca
    10261 atcacttctt tgaactgttc ggggacgagt ttttcgaaaa agtcgtttac gctgacaccg
    10321 cttggagctt taaattttgc catagtccat ttacctccat gaattttgct ataatatttt
    10381 atgtggattg ttgtttaccg gttggtaagt actagaaaaa aaataacctg tcaacttgtt
    10441 ttttcatttt tgagtttgac gtcggattct attttgttat aagttaggat aaacaataac
    10501 ttacaaagga atagagcgtg aaattgtttt cggcgaacag gggtagaata aaaaaataaa
    10561 aaatgaatat ttttatttac catttggttt ggtaaatgat atgatcaacg catgaatgat
    10621 atagagaaaa aattgaaatc atctttgcaa aaattaagta aaccttcggg cagccgctct
    10681 tcatccaatg agggcaatgt ccgggagcgg attttaaaag cagcccgcga tgttttcacg
    10741 gtctatccct tcaaggaagc cggcacccgc atgattgcca agcaggcagg tgttgagcat
    10801 cccatgatcc attattattt tggttccaaa gaaaatcttt tttacgccat ggccgaagat
    10861 ttgtatcatg aaactctttc cgtgcaggaa gcgtggcttg acggaatgga aaacatgtct
    10921 ctgaaagagg gctttgagct ttttctcgat cgtatgcttg attacaccat gcaaaaaccc
    10981 gatgccctgc gtatcgccgc gctgaatgtg gtgaatatcg gaaatttaga ggaaataccg
    11041 ggctaccgtt tcatcatcat gagtatggcg tctatgcggc gtatcattga agagaagctt
    11101 tcgttgaaag gatcaaatcg cgaaatcgaa atgtttattt actgctttta cagtctgatg
    11161 atctccctga tcggggcaag atcatgtcag gcccagatat taaacatgaa ccccctggag
    11221 gagaaatacc gcacatgggt taaggatgcg agcatgacgc tcttttatcc gtggtttaaa
    11281 aaaattcttg tcggccgcta acgcatccta cagatattgc agagactttc cggtttaatc
    11341 ttttcctgcc gttttacctt tcctggcagt accggttttc agggtgtgcc aatttagcct
    11401 tgacaaataa attctactgt gatttacgca ttaccctatt acttttagag gattcactaa
    11461 tgaaaaagac tttgaccaat aaaacaaata ccaaaagaaa gaaaacacac ggttttctcg
    11521 tcagaatggc atcaaaagcc ggccgggagg tcttaaatcg tcgccgggcg aagggaagaa
    11581 agcgtatcgc tgtttaaatt cggcatatca gatgccattt ttaaggatgg tcgggtcagt
    11641 atgcatgaag gaacaatctt atcggaaact ggaaaggata ggcagccgaa gcaggtttaa
    11701 aacaatttat aatcaggggg tgtggagaag ttcaagacat tttacaattg taacatgctt
    11761 aaacactcaa ggaatcaaaa gactgggaat tacggtcact aagaaaacgg gaaacgcagt
    11821 aaaacgaaac agaatcaaac gactagtcag agaatttttt cgcctcaata agaacctgtt
    11881 tcccgacaat tacgatgtcg tcatcatggc taaacgaaac ataccgtcct tgacgtataa
    11941 agaagcatgc gatgaattaa cgggactctt cacgcggcaa aacaatatat aatttatgct
    12001 cagaaagata ttcattaatt tttttgtttc atttattcat ttgtatcaag cctgcctgtc
    12061 acctttcttt tttgctccct gctgccggtt ttatccctca tgttcccaat atgcgattga
    12121 ggccatcaaa ctgtacggac cggcaaaagg ttcctggatg ggcataaaaa gaattcttcg
    12181 ctgtcatccc ctgaatcctg gcggttacga tcccgtcaag taaaaaatag agaggttgaa
    12241 aatggataag agaaccattc tggcggttgt cctgtcactg gctgtgcttt ttgcatatca
    12301 gatttttttt gccaaaccgc ctgtgccacc cgaaacggtt ccggttcggg aatcacatca
    12361 gacagcgcaa aaaccgaccc cgataaagga aaattctgtc gtcaagcaac aagcggtttc
    12421 tcccgatgcc tccacgacga aacagaaggt caaaaacgtc cttccggcac aagaagaatc
    12481 atcccgggac atcaaggttg aaacggcaaa ctacaccgct gtgttttcca ccaaaggcgc
    12541 ggctctgaag tcctttcaac ttaaggggta tcagaaagaa tgcacaaagt gtgtcggcga
    12601 tatctggccc gcgatcaaga attttgttac gggtaaaaaa cagccggccg gagaaaaaaa
    12661 caatgatttg atcgaggttg tgtcattaca gccggatatg ccttatccgc ttacgcttac
    12721 ttttcctgag tcatccggcg agattgccgc ggattccgtc tacaaaacgg acacagccga
    12781 gttgaatatg gttaagaaca gcaagaagca gcgtctggtg ttttcccgcg tgttcaatga
    12841 tgtcagggta gaaaagattt atatattcaa tcccgataat ttttcaataa cgctggaggt
    12901 caacatatcc aatctgaccg atgtgccgat aacgaaaatc ccgtatctta actggtacga
    12961 ctatgtggac ccgaaagaaa aagtagacgg ctacagcaaa gaaggtcctg tggcttacgt
    13021 gtcgaaatcc atcgaacgct ggaaatcggc ggatgtgtcg aaagaacaaa ccatcggtcc
    13081 cgatgtgtcc tggggcggcg ttgaaagaaa atatttcatg gcagtgatta ttccgcagga
    13141 tccgtcttta acaagtttca acatgtcccg ggatgatcac gtgattgccg tgaatctgaa
    13201 aggagcaaaa aatatcatcc cgccggagca gtccggttcg ttcaaatata atctctttat
    13261 cggtcccaag gactacgcca tgctcaaaaa actggggttg tccctggaag aggccattga
    13321 attcgattcg gtcattccgg gattgaagtg gctctccatc ggtctgttgc ttttcatcaa
    13381 atttattcat cagtttgtgg gcaactacgg tgtggctatt attattctga ccattctgat
    13441 caaggttctt ttctggccgc tcggaaatat cagctacaaa tccatgaaag agatgcagaa
    13501 gattcagccc caaatcaccg ctttgaggga aaaatataaa aacgatcagg ccaaactggg
    13561 acaggaaaca atggcgcttt acaaaacgca taaggtcaat cctttcggcg gatgcctgcc
    13621 gatcctgatc cagatccccg tgttcatcgg gctttacaac acactgctgt atgccattga
    13681 actgcgccat tctccatttt tcttctggat tcaggatcta tcggctaaag acccgtatta
    13741 catcacaccg attgtcatgg gggcgacaca atttattcag cagaaaatga cacccacgat
    13801 gggcgatcca atgcaggcca agatcatgct ggccatgccc atcattttca cttttatctt
    13861 tttgaatttc ccctcggggc tggtcattta ctggctggtc aataatatca taagcatcgg
    13921 acagcagata tacattaata aaaaattaac ggattgattc aagcgccggt tggacaaaag
    13981 aggatgaaaa atgagttctg aatttattga aatagaagaa aaaaccattg atgaagccat
    14041 tgagaaggcc tgtcagacgt tcggcgtgcc tcgcgaaaag ctcaatatcg aaattatctc
    14101 aggagaatcc ggaggctttc tgggattgtt ttccaaaaaa gcgaaaatca gagcgtccct
    14161 gctggctctg gatatcgatt tttccttctc ggataaaccc aaagaaaaaa cggacaagcc
    14221 cagggaaaaa aatgaacccc ggtccaaagc ggaccattac cgggaaaaaa gcgaagcaaa
    14281 ggttttgccc aaaaggaagc cggaaaaaga agtaaaagag atatcggaag aaaaatcgcc
    14341 ggtaaaaaaa gagcgggagg ctttcattcc caattccccc ctgtcgctca aggcgcagga
    14401 actgctggag ggcattctgc agagaatatc gccggaatac aaagcaaatg ttcaagaaac
    14461 tcaggaaaag atcattctgg gaatcgaggg cgacgaaagc ggcctgctga tcggcaaacg
    14521 cggacagaat cttgatgcgt tccagtacat cattaacaag gccatcaata agatcgacag
    14581 cgaacacaag atgattctga tcgattcggg agaataccgg aagagaagag aagattttct
    14641 gcttgatctg gcggaaaaaa taaagaaaaa agtcaaaaaa accaaaaagc cggtatccat
    14701 cgccaatatg aatgctcacg atcggcgcat tattcatctg gtcatgcagg aggacgaatc
    14761 actcgtgacg caaagccgcg gggaaggcaa gtaccggaaa attatcatcc ttccagcaaa
    14821 aaacggcagc agccggcgca cgaaagagaa aaagaaggat atcaaccaat cataataagg
    14881 ggaagtgcct gccgtgcttc gcagcgatac catagccgcc gcggcaacgc ctttcggcat
    14941 gtccggaata gccgtcatcc gggtcagcgg accggaagcc cttgcagtgg cgcgccgtat
    15001 tttcagaccg tcaaaccgtc catgcgactg gaaaagccat catctttacc atggtgatat
    15061 cgtttgcgcc gatggcgaaa cggtactgga tgaaatcctg atagctttca tgcgcaaacc
    15121 ccgctcattt acgggcgagg atgttgtcga aatcagctgt cacggaaatc ctgttatcat
    15181 ccaatccatt cttgaccaat tgcaaaaact gggatgccgg ctggcaggtc ccggagaatt
    15241 ttcacaacgc gcttttctga ataaccgttt tgatttgtcc caggcggaag cgctggcctc
    15301 gatgattggc gcccaatccg aaaaagccta tgccgtgggg ctcgcacagc tcaaaggatc
    15361 gctttccaaa gaaattgaaa cgatccgcgg gttggtgatc gaagcgctcg ccctcatgga
    15421 agcaagcatc gattttgcag aagataccgc tgaagcagag cttgtgcaaa taccgccgca
    15481 gatcaatcag gcaatggaaa gaatgcagag gcttcttaca tcctaccgct ttgccagagc
    15541 ctattcggaa ggtgttcatg tggtcattac aggaaagccc aacgtcggga aatccagtct
    15601 gctcaatgct cttgcgggaa agaaaaaagc gatcgtcact gatattccgg gaacaacacg
    15661 cgatctgatt gcggatacga ttcatatcgg cggtattccc gtgcacctga cggataccgc
    15721 cggaatccgg gaaccgcaaa acgtaatcga gcaggagggt attgcgctcg tctggaaaaa
    15781 actggccgaa gcggatattg tcataatcat gctggacgtg agtaaaccgt tgacagaaga
    15841 agataaaata acgattgata aaaacacgtc ggagaaaatc attgtcgccg tcaataagat
    15901 cgacttgccg cgttcgtggg aaacgaaaag cattgcgaac ctttttccgc ctcatacaaa
    15961 gtttctggaa atctcggcca agtccggctc cggactggaa gagctgaaaa gaacaatctt
    16021 ggatttatcc gttgacggtg aaacagaaag cacgtccggg gcgatcctga ccaatttacg
    16081 ccacaaacat gccgtcgagc aatctctgaa aaatattcaa aacgcgaaag aaagcgttac
    16141 cgccggtcta tcagcggaat ttatcgcgtt cgatttacgc gaagcgctgg atcatctgga
    16201 tgaaataacc ggcaaaaaaa tcaacgacga gatcctggat aggatttttt ccagtttctg
    16261 tatcggaaaa taatacgctg tctttcgcgt cctagagcgt tttgcaaaat cgttctttat
    16321 gaaaacaagg accgcggacg gcggcgaatt tgtaatcatt ataacattat gatagtaaaa
    16381 taacattcgg ctttatacgc agtgacgcat taaagatacg aacttatgca tggcatgctt
    16441 accgacatcg gcatttctgt tctggcggcg accgctctgg gctttgtatt tcagcttgtc
    16501 cgtcagcccg tcattctcgg ttatctggtc gccggggcgc tgatcgggcc ggcgatggga
    16561 ttcaatctgg tttccgaagc cgccagcatc gaggtcatct ccgagatcgg cctgatcctg
    16621 ctgcttttta tcatcggtct ggaactcaat ccgtccaaac tcctggcttc cggtcgtcag
    16681 ctgatctatg cgggtatcgg ccagtttatt ttctgcgtgc tgatcgggct gggatttttt
    16741 ctgctgcttg gttacgaatt gagccgaaac aatattgacg ccctttatct ggccctgttt
    16801 tgcgctctga gttcgacggc gattgtcgtt aaacttcttt atgacaagtt tgaactggac
    16861 accttccccg gacggatcac gctgggcatt cttatttttc aggacctgtg ggccattctg
    16921 attctggcgc ttcagccgca tttcacgaat ccgcaatttc ttctggtttt tatcgctctc
    16981 ggcaagagcg tggctctgct ggccatcggt tttttgctca gcaagtacct gttgagctgg
    17041 atttttaaaa aaatatcaca aacgccggaa atggtggtgg ccatgtctat ggcgtggtgc
    17101 gctttgatgg cgggtatcgg ttcatggatc ggtctttcga tggaaatggg cgctctgatt
    17161 gccggtgtcg cgatttcgac gtttccctat agcatgcatg tgacggcgaa agttcttccc
    17221 ctgcgcgact tttttctgac ccttttcttt ctgtccattg gtatgaaaat tccttttccc
    17281 gatgccgcga ttctgggcat ggcgttgatc attgtcctat ttgtgattgt ttctcgtttt
    17341 ttgacgattt atccgctgct gacgctggcg ggcagcggca ggcggaccag tttcatcgcc
    17401 agtctgaatc tcgcccagat cagcgaattt tctctggtgg tggccgcgct cggcgtgcat
    17461 tacgggcaca ttgatcagcg gatgatgtcg ttgattatct acgccatggc catgacgtct
    17521 gttctttctt catattttat caaggggaat cacgcggcgt atctggtatt caaccgtttt
    17581 ctggagaaaa ggggatttcc ggccaggacg aaagaaaatc atgatgaaga ggaaaaaaat
    17641 gtctacccca tcgtgattct gggattccac cgcggcgcgc gggaatttat cggccggctg
    17701 gagacggaga ccccggattt attgaaaaag cttctggtga ttgatttcaa tcttgaaaat
    17761 ttaaaagaat tgagcaataa aaacataaaa gggattttcg gcgatatcgg cagcatggat
    17821 acgctggagc acgctcatat aaccgcagca caaataattt tttcaacgat tcccgacatg
    17881 ctcctccggg gaaccagcaa caaggctctg gtgacttcat gccgtgcctt ggcgcccagc
    17941 gcctacatcg ttgctacggc tgattcggcc gatcagattg atgtcttgag gaaaagcggg
    18001 gcggatgagg tgctgctgcc ttatgtcctg attggagaaa aactgacgag gtttgttgag
    18061 cagacctgcc tgacggcgga gtcctgcaaa ctctaaaacg taaccggtgt agatatctgt
    18121 ttatgaaatg gatgggtaaa aaagttggac tggtactggg cggagggggc gtccgcggtt
    18181 tttctcatat cggcgttctc aaggttctgg aagaagaagg catcgacatt gatctgattg
    18241 taggaaccag cgccggtgct ttgatcggag gcgcttatgc ttccggccag gctcccgatg
    18301 agatcgagag caaaatcgac gcttatctgc aaagtcctga gttcgacgac tccaggatga
    18361 aatccattgg actgtctttt acacaagagg agaaaaacat tctcaagaaa gcacaacaat
    18421 ttatcatgaa tcgcgttctt tttgttcagg cctttttcaa accgtccatt ctgccttccc
    18481 atgacttcct ctctctgatg aactattttc tgccggatct cgatattaaa gaaacacgaa
    18541 ttcctcttca cgtggtttcc accgatctga tctccggtaa aaaaatcgtt ttttccgaag
    18601 gatcgttgcg caaggttgtg ctggccagtt gtgcggttcc gggagccgtc gagccggttc
    18661 gttacggaga ctggctgctg gctgacggcg gcattaccag tctggtgccg gttcacgccg
    18721 cacgggaggc cggagccgac gtcgtcatcg ctgtgacggt ggatcgagat ttgcaaacga
    18781 atctcaggat cgaaacggcg aaggacgtcg ttttcagggc gggagaaatc acccccagcg
    18841 ttctggaggc ggcggaattg gcggatgccg acgtgatcat ccgtccccag gtcggcaatc
    18901 ttcactggat ggatttcaaa cgggccgtgg accttgtcaa ggcgggagaa agcgccgccc
    18961 gcgattccct gaaaagcatt cacgcttctc tgccaattta tcggcgcatt acacgcttgg
    19021 ctggaaaatt atttaaacaa tcacggctct aaacccgatt tccggtttac gtttttttag
    19081 attgaaccgg aacggttttt caattatctt ggacaggcaa tgtgcttgat tcagcttaaa
    19141 ggaaggacgg agattatcat ttaattatct gattttgaac atcattttat taaggtcatt
    19201 tagttcttga tttctgtttt ggtttggtat agataacgcg cctactattt ttaggactcc
    19261 ttgcccgtcc aatcgggctt tagagccgaa aggagaaaac attgcaaaga tatgaagttg
    19321 tggctatcgt cctggccgat ttaattgagg acgagatcaa cgcgttaatc gaaagaagtc
    19381 agaccatcat taccgaccgc aaaggtgtca ttgccaaggt tgacaagtgg ggcaaacggc
    19441 atctcgccta tgaaatcaaa aaacagagag acggttatta ttttctcatc gattttgccg
    19501 gcaacggttc cattgtcaac gagattgaaa gaaattacaa gatcgatgat cgggtcatta
    19561 aattcatgac ggtgaagaag gaaggtgcga ccactgccga aggtattcag caggaagccg
    19621 ccgcagccga agcaaagcgc gttcaggcca aggtagaggc ggaagccgct gctgccgaag
    19681 gcaaacagat taatcaaatt gagaaggcgc cgcgggaatc cagaccgcgc gttagaaaag
    19741 atcacaacaa agaagaaact caaacgaagg gggaataagc catggcaatg gaaacacaaa
    19801 acggagaaaa acaaggaaga tatccccaga agaagttttt tcacagaaaa aaattctgca
    19861 ggttctgctc ggattccaat attaagattg attataaaaa tactgcgatt cttaataatt
    19921 tcgtaacgga acggggcaaa atcatgccgc gccgcatcac cggcaactgc gcttcccatc
    19981 agcgggaact ggcactggcc attaaacgcg cccggcagat tgcgattatg ccttatgtga
    20041 cggctgacgg tagataaatc tgttccgcag tttttttgtc gttagaataa tggtgtgatg
    20101 attatttttc aaaactgagt cattcgtttc ggtttaaaat caattcaaca acatttcagt
    20161 caggtaacgc cgcgggcggc gttaccttct ttttcagaaa aagcgacggt taacggggtg
    20221 ttgcatcgtg agcagttttt ctttctttaa aaaatccgga gcacccgcgt ccggcaattc
    20281 tttttccggt ctcctgtctg ctgttggtct gtttgctttt gccgccgttt ttgttccgct
    20341 tgtcggcccc gtttttcttt ttctgcttcc gatgattgcc ttcgtcaatg gagcaaccaa
    20401 cggtatcttt aaaacagcac tgtgtctttt attttcttta gcggttctta tgcttttatc
    20461 cgtcgggctg aatctggatg tgccggctgt cgcgattttt accatgggaa tggccggatt
    20521 gcttatggcg cacatggcgg ctgaaaatta ctccatcgaa aaaatagtta cgggaccggt
    20581 tttattgatt tttggagcga ttggttttta tttcgtttac gatgctgtgg gcctgggggt
    20641 gaatccctgg ctgctggtga agaattttat aaccgtgacc atcagcgaaa caatcaaatt
    20701 ttacagccag atgcctttaa aaccggacga tatcagcatg atcaaagata atgaaaacaa
    20761 tatcatcaac agcctcatcc acattttccc gtccctgatg gtcattctgt ccgtattcat
    20821 catctgggct aatcttttac tgggcaaaac ttatctgaga cgattcggca tcgtttatgc
    20881 gcaaatggca atactggcgc gttggaaagt gaccgactgg gccatatgga tttttatcat
    20941 atccggcctg ctattgttca ttccacaaaa gaactttaat ttcattgggt taaatgtttt
    21001 tctgatcgtc tgcttggtgt atctgctgca agggctggcg attgtcagct ttctcttcca
    21061 gaacaaaaac gtaccttttt taatccgcta tattttttat tttttaattg cagtgcagca
    21121 aattcttatg atacctattg cggtcatcgg gctgtttgat gtatggtttg atttccgcaa
    21181 gcgttttcaa aaaaatcatt tatgaacata atgatcgcaa tactattttt ataaaaacgg
    21241 aggattttca tgaaggtcat tctgaaacaa agtgtgcgtt ccctgggcaa agccggaacg
    21301 gtggtgaaag tcaatgacgg gtacgcccgt aattttttaa ttcccaaggg actggcgatt
    21361 gaagccgatg aaaaaaacat cagactcttc gaacatgaaa aaaacaatat tctgaagaag
    21421 gccgcaaaag aacataagaa cgcgcaggat ctggctgatg ttctgtcccg cgaaaccatt
    21481 accattgccc gcaaggtcgg tgatcaggac aaacttttcg gttcggtaag cgcaaaggat
    21541 atcgaggccg cgttaaaaga aaaaggatac gacattaacc ggaagatgat catgcatgag
    21601 cacggcgagc acattaaaga actcggcgag ttcgcggtca aaataaaact ggctcatgat
    21661 gttgaggcac agatcacgtt gaaggttgtc ggcgaatcat aatgaaagaa aaagatccat
    21721 cgcaatataa actcccgccg cagaatctcg aggccgaaca gtctgtcctg ggcagtattc
    21781 tgctggagaa cagtgccacc aacagggtct tcgaaatttt atccgagagg gatttctact
    21841 ccgaggctca tcggaagatt ttcattgtga tcaaggatct gtccgaaaaa aacgaaccgg
    21901 ttgatctgat taccctgagc aatgccctca aagataaaag ccttctggat tctgtcggcg
    21961 gtacaaccta cctggcttct ctggtggaca acgttccatc ggcggccaat gccgccaatt
    22021 atgcgaaaat tgtcaaagag aaggcgattt tacgcggtct gatcacgtcg gcaacggaaa
    22081 ttatcgacaa ctgttacgaa accggttccg atgtggatac tattctggat aaggccgaac
    22141 acagcatttt cgaaatttcc gaaaacaagg tgcgccagga ttattttccg atccgggata
    22201 ttattaaaga cagtttccgc tctatcgaag atttgtattc ccgccagcag ttgattaccg
    22261 gcgtgccgac cggcttcgga aagattgatg atttgaccgc cggcttgcag aaatccgaac
    22321 tgattattat tgcaggacgt cccagcatgg gcaagacggc gtttgccctg aatatcgcac
    22381 tgcacgccgc aatggaagcg cagattccga ctgccgtctt ttcgctggaa atgtccaagg
    22441 aacagcttgc ttttcgtctt ctgtcctctg aggccagggt ggatgcccag cgcttacgaa
    22501 aaggttttct gggagaaaca gactggccga aactgacgac tgccgccggc cgcctttcgg
    22561 acgcgcctct ttacatcgat gacacgcccg ctattaccgt tctagaaatg aaggccaagt
    22621 cccggaggct caaagcggac aagggcttgg gactggttgt tgtggattac attcagctga
    22681 tgcgctccag ttacagtcac gattcacggg aacgggagat atcggatatc agccggtccc
    22741 tgaaggcgct ggccaaagaa ctcaatgtgc cggttgtggc gctttcacag ctgaaccgtc
    22801 aggtcgaaag ccgcaccaac cgccggccgc agatggcgga tttgcgcgag tcaggcgcca
    22861 ttgaacagga tgccgacgtc atcgccttta tttaccggga tgaagtgtac aacaaatcgg
    22921 aggacaatat cgacaaaggt attgcggaaa tcatcattgc caaacagaga aacggcccca
    22981 ccgacaccgt caagctggcc tttgtggaca aatacacaag tttcgaaaat ctgtcacggg
    23041 tggaactgcc tgaaggacag cattgatccg aatatccggt cgtgcatcac cggccaatga
    23101 atgccctccc attttcccgg gattaatccg gaagggcact tcataaaaac tcctagggtg
    23161 cagtgcttca ataaaaaacg atatctttga tctggtcaag taaaaacgga cacccggtta
    23221 agctgctttt gccaacgtaa g
//
LOCUS       DAOSME010000036        23112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2779_length_23112_cov_18.566466, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000036 DAOSME010000000
VERSION     DAOSME010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23112
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2779_length_23112_cov_18.566466"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..628
                     /locus_tag="PLG98_06765"
     CDS             257..628
                     /locus_tag="PLG98_06765"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOE32993.1"
                     /translation="MKLRDVKEILDAHVVVGEEKLDMDVHTAFGADLMSDVMAFAKAG
                     SLLLTGLTNPQVIKTAASRNIAAIIVVRGKEPTTEAIAMAKDLQIPILTTNYILFETA
                     GILYSKGIVGCLKKVESGFSK"
     gene            868..1242
                     /locus_tag="PLG98_06770"
     CDS             868..1242
                     /locus_tag="PLG98_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOE32994.1"
                     /translation="MKLREVKDILNADVIVGEDKLDIEVQTAFGADLMSDVLAFAKAG
                     SMLLTGLTNTQVVRIANVLDIAAIILVRGKKLSTEAISLATDLHIPILTTKYILFETA
                     GRLYAKGIVGCLEKVESGSDRI"
     gene            1229..1672
                     /locus_tag="PLG98_06775"
     CDS             1229..1672
                     /locus_tag="PLG98_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32995.1"
                     /translation="MIESDKNQHIFTFDVEGGNFNAAGTVSTRIKSLLKQLQMPDDVV
                     RRSAIVSYEAEINIVSYAKKGVIRITVSPDQVMIEADDTGQGIPDINLAMQQGYSTAN
                     HLIREMGFGAGMGLFNIKSYSDKFDITSEVDKGTFLRIIIKEHVT"
     gene            1683..2024
                     /locus_tag="PLG98_06780"
     CDS             1683..2024
                     /locus_tag="PLG98_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE32996.1"
                     /translation="MDIGIIAEKLNLKFMSASDRKANNVTGGYTGDLLSDVMANSHGG
                     DVWITRQIHQNIVAVATLKEHAGIILVNACEPAKDTLEKAVQENVPILVSDLSAFELT
                     GRIYNLLHSDK"
     gene            2090..2827
                     /locus_tag="PLG98_06785"
     CDS             2090..2827
                     /locus_tag="PLG98_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOE32997.1"
                     /translation="MMKVFNCDLHIHTCLSPCAELDMHPAALVEKTIDAGLDVIAVCD
                     HNASENVPYVIKAAQGTHLKILPGMEITTSEEVHFLALFDSLSDLIALQHFVDLHLPG
                     KNDEDRFGVQAIVNEHGEVEGLSDKLLIGATDVALNDLLTVVHHHHGLAIASHIDRES
                     FSVLGQLGFIDDSIHFDALEISYATGIRQARERYADLKKYAFITSSDAHYLKDIGRAT
                     TKIMINEPTLSELKMAFSRENGRYVVE"
     gene            3029..3577
                     /locus_tag="PLG98_06790"
     CDS             3029..3577
                     /locus_tag="PLG98_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE32998.1"
                     /translation="MLELAAHILDIAENSVRAGAKLVNITIIEDDKKNLLSIEINDDG
                     HGMTEEEIVKALDPFYTTKKVRRVGLGLPLLAEAAKRAGGDLRLKSVKGKGTKLKVTF
                     QLDHLDRQPMGNITTTIRNLIAGNSFVDFLYKHRHNDRRFSLDTRTIRKEIDDLPINH
                     PEILKYIRDVIDDGLEDIQSRA"
     gene            3606..4100
                     /locus_tag="PLG98_06795"
     CDS             3606..4100
                     /locus_tag="PLG98_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE32999.1"
                     /translation="MKSEDNELLKEFTPEQVDQLNKIIKKHKGKPGSLIPVLEEAQVC
                     LEYLPVSVQKKIAKELNLPLSRVYGVVTFYSFFTMTPRGKHTVRVCLGTACYVRGGKA
                     LTETLQKEFGIHEGETTADRMFTLESVRCLGACGLGPVVVVDEDVHGRVKPSKLKEIL
                     AQYK"
     gene            4128..4511
                     /locus_tag="PLG98_06800"
     CDS             4128..4511
                     /locus_tag="PLG98_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOE33000.1"
                     /translation="MAKLTINDLKKIKEKVHKEMSLRDGERRVKVTVHMGTCGIASGA
                     KEVMDTLLQEIETAGANDVVVTTSGCMGLCSREPLVTVEILNQEPIKYEYMNSNKMRQ
                     VFKRHIQGGEIQTPFVLARGAEVIK"
     gene            4577..>6747
                     /gene="nuoF"
                     /locus_tag="PLG98_06805"
     CDS             4577..>6747
                     /gene="nuoF"
                     /locus_tag="PLG98_06805"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE33001.1"
                     /translation="MKVLRAHLLICGGTGCQASGSPAVKKALLEELDKKKLTEEIKVV
                     ETGCNGFCALGPIMVVYPEGVIYISLKPEDMAELVEEHLVKGRIVQRLLYREPTTDQI
                     IPTMQDIPFFAHQELRVLKNRGLIDPEKIEEYIARDGYAGMAKALTEMTPEQIVQEIL
                     DSGLRGRGGAGFPTGLKWKFAAQSKGDVKYVLCNADEGDPGAFMDRSVLEADPHAVLE
                     GMVIAAKAIGSKHGYIYCRAEYPLAIHRLNVAIDQAKKAGLLGKDILGTGFDFDLEIY
                     QGAGAFVCGEETALMTSIEGKRGMPRPRPPFPAVAGLWQKPSILNNVETLANIGQIVL
                     RGAKWYASIGTEKSKGTKVFALTGDVANVGLVEVPMGTKLGTIVHDIGGGIPKGKKFK
                     AAQLGGPSGGCIPIQHLNAPVDYEKVAELGAIMGSGGLIVMNEDKCAVDMARFFMDFC
                     QDESCGKCTPCREGTKRMLEILTNITQGKGKEGDIELLEEMAQVIKNAALCGLGQTAP
                     NPVLSTIRYFRNEYEEHIRDHRCRAAVCSAMFKSPCQHTCPIEMDIPAYIALIRAERF
                     DDAYKILLQTNPFPSVCGRVCDHKCQTKCRRGNLDEPVAIKFLKRFITDNAPRPKVDP
                     VLVSRKEKIAVIGAGPAGLTAARDLALRGYKVTVFEELPKPGGMLYWGIPSYRLPRNI
                     LDGEIDDIRALGVDIKLNTRVGKDITFAKLEKEFDYVYLATG"
     assembly_gap    6748..6847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6848..7439
                     /locus_tag="PLG98_06810"
     CDS             <6848..7439
                     /locus_tag="PLG98_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOE33002.1"
                     /translation="LVAPLNVEGAKGKVTGITCQRMKLGDFDSSGRKKPVPVEGSEFT
                     LKVDAVISAIGQVPDMSFIDAKRGVEINKWDCFAVGKVYKSGTTNPRYFAGGDAVTGP
                     DTAIGAIAAGHQAADDIDAAIRKANGEPAYEKPALEKIDIPLIIDEETVEAPQMAMPE
                     LHHETRKTNFVEVELGFKREDAIKEACRCLRCDAAV"
     gene            complement(7550..8470)
                     /locus_tag="PLG98_06815"
     CDS             complement(7550..8470)
                     /locus_tag="PLG98_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33003.1"
                     /translation="MENNRKKKENLGNRQDQNLRHYFETQKELFESWNGNYEKFVTST
                     HNKYKMHYQKEFADFSAFIDYLIKDDEQTVQDTSSSVADDHKKIDSLEAEFDGIFRAW
                     IDQATQERNTDDEDEGKGEWLDYVESQAIATFYSVLINYKEHIENVYHRQLRGKKLGY
                     ARKWMNDDPRVERLIIKYKVCEILNELEKKDSRSAKTKKPFRYLFQLEEPKYQGRYPY
                     ACFITNQDFYNAILQALDIKKITLQKYFKALCQVGAIKKLQGAGRVDIRKNETLYALG
                     YFSGLKGTNYKLNRFLTQKSTNKFLKFKLS"
     gene            8843..9088
                     /locus_tag="PLG98_06820"
     CDS             8843..9088
                     /locus_tag="PLG98_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33004.1"
                     /translation="MPRWTYKTNIIDLDQVIPPDKVQCDLSGDCIVTDLQGGGLPHFK
                     EMLDKEGEGEWELVQCQYHGGKLLCIWKKEIKEAFAS"
     gene            9234..10115
                     /locus_tag="PLG98_06825"
     CDS             9234..10115
                     /locus_tag="PLG98_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOE33005.1"
                     /translation="MAFNAPAKMVGLVGNAGVYKSKLSVFQFLIRGFMAGAYIAIGGA
                     LCTVCSTGVAPILGPGIGKLIGGAVFPVGLIAIVLTGMELFTGDCMLVPMAAMMKRCT
                     WGSVFRNWFWVYIGNVVGSFFYAYMMAVGPFVDGTADGTLVVNAFGMTAINISVAKIL
                     TYKAVGTAGLWSCFMKAIGCNFLVNIAILLAITADDVISKFFGIWFPIMAFVATGFEH
                     CVANMYFLPTGKFLTDWYPATIEKVGGLLAANGGLSWGDIWFWNIIPVTIGNIVGGFL
                     FIGVAYFLMFRNELPKD"
     gene            10127..10594
                     /locus_tag="PLG98_06830"
     CDS             10127..10594
                     /locus_tag="PLG98_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOE33006.1"
                     /translation="MSLPAWITFFICAAAVFIHYMQVRNTRFLLFAIPALLALLIIPM
                     LLAWMSRRTLNKAAQENVSKAKLYKIGKVTPAMTGQVVSIKGHVKKISFRWLNRPHFH
                     VEDSTGRIRVIMFTAPDDNIREGDPVDILGIVIKNIFKRGTPAISAVSVKKNE"
     gene            10621..10770
                     /locus_tag="PLG98_06835"
     CDS             10621..10770
                     /locus_tag="PLG98_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33007.1"
                     /translation="MNSREIIFLIGFLILGITPVFAQQLPGGATELIMLAGMLISTST
                     AFVKS"
     gene            11269..12561
                     /locus_tag="PLG98_06840"
     CDS             11269..12561
                     /locus_tag="PLG98_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOE33008.1"
                     /translation="MANENLESKRWLIAGAAVIIQLCLGTVYAWSVFKNPLMQMHGWE
                     GKSVQYTFMLLMLIIGLAAAFGGTLVDKKGPRFVATIGGILFGIGTLVAGYADQVGNI
                     YLLYLGFGVIAALGNGFGYVTPIATLIRWFPDKRGLVTGLAVMGFGAGAFFMGKIAPV
                     MIKSFQQIDPAGTMIASGVANTWYIWGIIFLILVTGSAQLFKNPPAGWLPKGFTPAAT
                     TVSAADSFTFGEAVKTRQWWMLWAMLCLNVSAGLGLISQHSPLAQDIYKKTYGLTGDL
                     TPEQVAIVAAAGGAVVAYAAIFNGLGRLFWAKISDNIGRKMVFLIMFATQAVLYVLVA
                     KGFVATYWLFVIASCYLLACYGGGFATMPAFAADAFGPAYIGKVYGFMLTAWSTAGII
                     GPYVFEVFKPQALTIAAGLLTLGFVIALIYTPPKGKKA"
     gene            complement(12676..13107)
                     /gene="tnpA"
                     /locus_tag="PLG98_06845"
     CDS             complement(12676..13107)
                     /gene="tnpA"
                     /locus_tag="PLG98_06845"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOE33009.1"
                     /translation="MECQKQAHAVYYTRYHIVVSTKYRRKIFKEGVGEYLQKLILEVS
                     KYHPDIEILEVNTDLDHVHFLASIPPKYSVSEVVGKIKANTGQKMRKRFKFLDKVYWG
                     VEGIWSIGYFVSTVGINESVIRKYIEAQAKEDSGQAKLVME"
     gene            13198..14142
                     /locus_tag="PLG98_06850"
     CDS             13198..14142
                     /locus_tag="PLG98_06850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33010.1"
                     /translation="MINYYINSTEDYHKNELNSLGWELTVCNALDPMDSPCRRLIASQ
                     TSFGENLYRFLEEIIPVDTIRTILEIGGGMGYLMRDFLKFNPRLKATMIDISPYLLGC
                     QKDLLHSFEVDFLLADILTVPPTTLSSFDMAVMNENLGDLPTLVTGSKDDIYHGPAAG
                     VIEKIEAFNIKYNLQLGANQNEPVNIGALEMVEKLCLAGIKYIYLSEHSCEAAVPEKM
                     KNYLFFQTPGIPEMISLKGHREFTIKFSYLQKIAEMLNYTSRRGPFADFLPLDFNDRV
                     KTALRMLTPQSDNQEIIRQFVYDLFKYEYLILIRGDHK"
     gene            14139..14477
                     /locus_tag="PLG98_06855"
     CDS             14139..14477
                     /locus_tag="PLG98_06855"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOE33011.1"
                     /translation="MKKTVMIIFPALFFLLSVSVSPCHAFRCGNGLVTTGDTKNKVQM
                     TCGKPSAKEKRCLNSWTGKKAKCDKKVEVWYYNCGDNDFVYALIFEGNTLTGENSEGR
                     GHGPSECRGQ"
     gene            14781..16559
                     /locus_tag="PLG98_06860"
     CDS             14781..16559
                     /locus_tag="PLG98_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOE33012.1"
                     /translation="MTMDMLIVFGIILLAALLFASEKLRVDLTAIVIMVLLILTGIIS
                     PSEGISGFSNVATITVASMFILSAALQKTGSVNFLGTLSSKIFRFNFWVGLIGTMIFV
                     GAVSAFINNTPVVAVFIPIMLGVAADNQMSPSRLLMPISFASMFGGVCTLIGTSTNII
                     VSSVAVQHGLPALGMFEFTKMGLVFFAAGLVYMAVVGVRLIPLKKTETDLTKKYQMND
                     YLTDIVLLGNAKSIGKRVADSPLIKELDIDILEVIRNGRRLHVPVSDIVLMENDLLRV
                     RCDIKQLQKLKERMGIKMKSDCELNDENFRCEALSLTEVVVAPNSRLEGKTIKSSYFR
                     NVFHATALALRHRGQLFNTGFTDTILRAGDAILVETRKENYEALKNSNDFVIVSDIET
                     QQYRKSKIIPALLVIAGVIVTASLGIIPIVVGAMIGAILLVLSGCITLEEAYDAIDWQ
                     VIFLLGGVLSLGIALEKTGAAMYLSNNLIYVLGDLGPVAIVAALYLLTSILTELMSNN
                     ATAILLAPIAIAAANGIGVSPTPFLMAIAFAASASFMTPVGYQTNTMIYGVGQYRFSD
                     FIKVGTPLNIMFWILATLFIPVFFPF"
     gene            16568..17437
                     /locus_tag="PLG98_06865"
     CDS             16568..17437
                     /locus_tag="PLG98_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE33013.1"
                     /translation="MDGITALINQILNVFRIPLFHMGEAPFTLWTFIYLVAATMILTL
                     AASVLNKVIVTRLLTKSNIERGVRIAIGSIVKYIVLVVGFIIILQTAGINLSSLTILM
                     GALGVGIGFGLQNITNNFVSGMIILLERPIKVGDRIEVANVSGDVVDISMRATTIVTN
                     DNISIIVPNSQFISETVINWSHTDRSVRFNFPIHVSYREDPQAVRKLLLDIALNNPGV
                     LKDPPPDVLFSEFADSAMSFNLRVWTREYINRPGVLKSQLYYQIADRFREKGIEIPFP
                     QQDIHIKETARQS"
     gene            17633..18280
                     /locus_tag="PLG98_06870"
     CDS             17633..18280
                     /locus_tag="PLG98_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33014.1"
                     /translation="MRPVRIILLLMVMLWLMTTGCSEKLPILYSPLKDDYQLSPGFTD
                     REQGEKQLDEFFEEDQRLYRNLSRLTTVIDDAEDDPGDEAEIHSYPEAHNGILEGLTA
                     TEWFTAGRQAASDRRWDDALNAFNKAIALNPENEEIYFHRGNVYDALGNNKKAVSNFN
                     KAIKLNPLYVDAYLSRAFSYNNLAQANQALADIKKAARLGSLHAQKFLKKSNLAW"
     gene            18558..>18757
                     /locus_tag="PLG98_06875"
     CDS             18558..>18757
                     /locus_tag="PLG98_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33015.1"
                     /translation="MRKTIIATVMMMLSAFFITTAQADIRPGSYTVTPFIGGFIFEGN
                     EYYRGFDNQKNGRNTLDDTYAVG"
     assembly_gap    18758..18857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18858..19336
                     /locus_tag="PLG98_06880"
     CDS             <18858..19336
                     /locus_tag="PLG98_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE33016.1"
                     /translation="KYSVTDKIDLRADVRHVLPLNERYNDLLYSFGVTFSFGGAEKGK
                     KLLDIKFDFDKTDIKAAYHKDINAVTKLMQKYPDLNIVTEGHTDNIGTPEYNKNLSNK
                     RADAVKKYMVEKGGVAAGRIDTKGLGEGSPAASNDTEAGRAENRRVEAAVDYTGKK"
     gene            complement(19482..20183)
                     /locus_tag="PLG98_06885"
     CDS             complement(19482..20183)
                     /locus_tag="PLG98_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE33017.1"
                     /translation="MIKKEMIYRNPLVKLGYDNEDILSTGGFGAVLAYAGVGKTALLV
                     QLALNMMLREHSVLHVSLNDAVSKVDLWYQEIFHDIAEKFGIAEVNEYWDKIQPYRFI
                     MTFKVEGFSAPKLEERLTDLMEQNIFKPHTVIIDGFRFDEAGRGQLVQLKELAGKYSM
                     GLWFTVHTHRHEPPQENGLPLSFLHIADLFEVIVQLATKGDEVYITSVKGQPKNSAQN
                     DLLLDPATMLIKESQ"
     gene            complement(20266..22353)
                     /gene="fusA"
                     /locus_tag="PLG98_06890"
     CDS             complement(20266..22353)
                     /gene="fusA"
                     /locus_tag="PLG98_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE33018.1"
                     /translation="MKIDDQKIRNIGISAHIDSGKTTLTERILYYTKRIHAIHDVKGK
                     DGVGATMDSMDLEKERGITIASAATYCEWKGHEVNIIDTPGHVDFTIEVERSLRVLDG
                     AILVLCAVGGVQSQSITVDRQMKRYRVPCIAFINKCDRSGANPFRVISQLKSKLGHNA
                     VAMQIPIGLENDVQGVVDLVSMKALYFDGDNGETVREAEIPSHLLEEAKAKREELIDA
                     ASMFSDELTEAILEEKEVSADLIYSALRKGTLQREITPVYLGSAYKNKGVQPMLDGVT
                     YLLPCPSDIENVAIDMDKNEETVVLNNDTEKPIVALVFKLEDGQYGQLTYIRVYQGTL
                     AKGSTIVNVRTGKKVKVGRLVRMHADQMEDVESLRAGYIGALFGIECSSGDTFTSPDV
                     NLTMISMYVPKPVISLAIVPKDNKSQVAMAKALNRFAKEDPTFKTYVDHETGDTIIEG
                     MGELHLDIYVERMRREYGADVSTGNPRVAYRETITQRADFNYTHKKQTGGAGQFGRVA
                     GYLEPIADTDFVFENKIFGGAIPSQYIAACEKGFKQSMAKGPKLEFPVTGVKVVINDG
                     ASHAVDSSDMAFQAAARGAFKEAYLRAKPVIHEPIMKVVVETPAEFQGSVMGLLNQRR
                     GMIIGSQDEDVMCVIEAQVPLAEMFGFSTVLRSATQGKAQFTMEFALYRQVPQSIAEK
                     IALEAAQNKKTAA"
     gene            22663..>23112
                     /locus_tag="PLG98_06895"
     CDS             22663..>23112
                     /locus_tag="PLG98_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE33019.1"
                     /translation="MYEQILPNLYRIIIPLPNSPLKDLNSYVIKADNRNLIIDTGFNR
                     GVCYEAMENGLQALEIDLSRTDFMLTHMHSDHTGLVSRLFTETSKVYFSRIDSEVFNE
                     NIDWQAMLDYALHNGFELEELIRAAESHPGFKYRPRKIPDFTMLDDGS"
BASE COUNT         5973 a         5500 c         5360 g         6079 t
ORIGIN      
        1 caccggccct ctgaacccca ttcagatacg ctaccagact gcgccacgcc ccgatgaaat
       61 acaaattgac gtgcgtcatt accattacgg ccaacccctg tcaagggtaa ataaattgac
      121 gcttatcgca acgatatttt tatgtcgtga gatggattat tccccttcat tcataaaaaa
      181 atcttaataa ttaattgaca ctttttatgt ttttatgcct ataataaaaa agttaacatg
      241 acacaaagga gtttttttga aactaagaga cgttaaagaa atattggacg cccatgtcgt
      301 tgtcggtgaa gaaaaactgg acatggatgt gcatacggct ttcggcgccg atttaatgag
      361 tgatgttatg gcctttgcca aagcaggcag ccttttgctg accggattga ccaatcccca
      421 ggtcatcaaa accgctgcca gtcgaaatat cgccgccatc atcgttgtcc ggggaaaaga
      481 gcccacaacc gaagccatcg caatggccaa agatctgcaa attccgattt taaccacaaa
      541 ttatatttta tttgagacgg ccggcattct ttacagtaaa ggaattgtgg gctgtctgaa
      601 aaaagttgaa agcggttttt ccaaataaaa cggtcccccg gttttctcag aacattcaaa
      661 atatcgaaga tccatttttt aagttatttt taaaagaatc acttgaaaac acgtcggtgc
      721 gtgtcgaaat ctctcttttt ctgttcgttt tttttgattt taacaaaatg atttcattga
      781 cagtaaagtc tcttctatga taaaagattt ttaatttaat tttagatatt taaaacgaaa
      841 ataacctttt tcagcaagga gataatattg aagcttcgtg aagtcaagga catactcaat
      901 gccgatgtga ttgttggaga ggacaagctg gatatcgagg tgcaaacagc ttttggagcg
      961 gatctgatga gtgacgttct cgctttcgcc aaagcgggaa gtatgctgct tacgggactg
     1021 accaatacac aggttgtccg aatcgccaat gtcctggata ttgcggcaat cattttagta
     1081 cgcggcaaga aactttccac cgaagcgatt tctctggcta cggatcttca tattccgatt
     1141 ttaacgacaa aatatatttt atttgaaacg gccggtcgtc tttacgcgaa aggtatagtc
     1201 ggctgcctgg agaaagttga atccggcagt gatcgaatct gacaaaaacc agcacatatt
     1261 cacgtttgac gttgaaggag gtaatttcaa tgccgcggga actgtttcca cccgcattaa
     1321 atcccttctg aagcaattac aaatgcctga tgatgtggtc aggcggtcag ccattgtttc
     1381 ctatgaagcg gaaatcaaca ttgtctccta cgcaaaaaaa ggggtaatcc ggataacggt
     1441 cagtcccgac caggtaatga tcgaagccga cgatacgggt cagggaatac ccgatatcaa
     1501 cctcgccatg caacagggct attccacggc caatcatctg atcagagaaa tgggtttcgg
     1561 cgccggcatg ggattgttca acatcaagtc gtactcggac aaatttgaca tcacatcgga
     1621 agtggacaag ggaacatttt taagaattat cattaaagaa cacgttacat agaggatgga
     1681 ttatggacat cggaatcatt gcagaaaaat taaacctcaa attcatgtcg gcgtccgaca
     1741 gaaaagcgaa taacgttaca ggagggtaca cgggggatct gttaagcgat gttatggcca
     1801 attcacacgg aggagacgtc tggatcacca gacagattca tcagaatatc gttgccgtgg
     1861 ccacgctgaa agagcatgcc ggcatcattc tagtcaacgc ctgcgagccg gccaaggaca
     1921 cgctggaaaa agccgttcag gagaacgtgc cgatactggt atcagacctt tcagcatttg
     1981 aattaaccgg cagaatctat aacttgctgc attcggataa gtaagagcga attttttatg
     2041 tctccccggc acaaagctgc ctgatgttgt actgtcccgt ccggaaacca tgatgaaagt
     2101 ttttaattgc gaccttcaca ttcacacctg cctgtcgccc tgcgcagaac tggacatgca
     2161 tcccgccgct ttagtggaaa aaaccattga cgccggtctg gatgtgattg ccgtctgcga
     2221 tcacaacgct tcagaaaatg ttccctatgt gatcaaggcg gcacaaggca cgcacttgaa
     2281 aattcttccg ggaatggaaa taacgaccag cgaggaagtg cactttcttg ccctgttcga
     2341 ttccctttcc gatttgatcg ccctgcagca tttcgtcgat ctgcatctcc cgggaaaaaa
     2401 tgatgaggac cggtttggcg ttcaggccat cgtcaacgaa cacggcgagg ttgaaggact
     2461 cagcgataaa ttattgatcg gagctacgga tgttgcgttg aatgatttgc tcaccgtcgt
     2521 tcatcatcat cacggactgg cgattgcctc gcacatcgac agggaaagtt tcagcgtcct
     2581 gggacagctc ggttttatcg acgacagcat tcattttgac gctctggaaa tatcgtatgc
     2641 aaccgggatt cggcaggcgc gggaaagata cgcggatttg aaaaaatacg ctttcatcac
     2701 ctcatccgac gcccattatt tgaaggacat cggcagagcc acaacgaaaa tcatgataaa
     2761 cgaaccgacg ctgtccgaat tgaaaatggc tttttcaagg gaaaacggcc gttatgttgt
     2821 ggaatagtcc atgcgacagc atcctctgta tgcatcgtcg catcgttgac cggacccgac
     2881 ctttttccgc tgatgcggcc gagctgttca tgccgcgctt ttagacttca tgccttacgc
     2941 aatcctgaag gcatttttac agatctcgtc gggttttgcg atttgcttcg gaaacccgga
     3001 gatccatgaa acgggagatt tataactcat gctggaactt gccgcacaca ttctggatat
     3061 tgccgaaaac tctgttcggg ccggcgccaa actggtgaac atcaccatca tcgaagacga
     3121 caagaaaaat ctgctttcca ttgaaatcaa tgatgacgga cacggcatga ccgaagagga
     3181 aattgtcaag gcgcttgacc ctttttacac cacaaaaaaa gtcagacgcg taggactggg
     3241 ccttccgctt cttgcggaag ccgcaaaaag agcgggcggc gaccttcgtc tgaaatcggt
     3301 caaaggcaaa ggaacaaaac tcaaagtcac ctttcagctc gatcacctgg acaggcaacc
     3361 gatggggaat atcactacga caatacgcaa tcttattgcc ggaaattctt ttgttgactt
     3421 tctttataaa cataggcata atgaccgccg gttctcactg gatacgagaa caatccgcaa
     3481 ggaaatcgat gatttaccaa taaatcatcc ggaaatttta aagtatatta gggatgttat
     3541 cgatgacggt ttagaggata ttcaatcccg cgcgtaacag accaataaga ttgaggagga
     3601 aattgatgaa gtcggaagat aatgaactct tgaaagaatt cactccagaa caggtggatc
     3661 agctaaacaa aattattaag aagcacaaag ggaaaccggg gagtttaatt ccggttttgg
     3721 aagaagcgca ggtatgtctg gaatatctgc ccgtttccgt gcagaagaaa attgccaaag
     3781 aactcaacct gcctttgagc cgcgtttacg gtgtcgttac attttattca ttcttcacca
     3841 tgacgccaag gggtaaacat accgtccgag tctgtctggg aacggcgtgc tatgtgcgcg
     3901 gaggaaaggc actgaccgaa accctgcaga aggaattcgg aattcacgaa ggcgaaacga
     3961 cggcagacag aatgtttacg ctggagtccg ttcgctgcct cggcgcctgc ggtctcggac
     4021 cggttgtcgt tgtggatgaa gatgtccatg gacgcgtcaa gccatccaag ttaaaagaaa
     4081 tactagccca atacaaataa aataaaaaaa gccgggagga aacgtcaatg gctaaattga
     4141 ccataaatga cttaaaaaaa ataaaggaaa aagttcataa ggaaatgtct ctccgagacg
     4201 gcgaacgtcg tgtaaaagtt accgtgcata tgggaacctg cggcatcgcc tcgggagcca
     4261 aggaagtgat ggacacgctg cttcaggaaa ttgaaacagc cggagccaat gatgtcgtcg
     4321 tcaccacatc cgggtgcatg ggattatgca gccgcgaacc gctggtaacg gtagaaatcc
     4381 tgaatcagga accgatcaaa tacgaatata tgaattcgaa caaaatgaga caggttttca
     4441 agagacatat tcagggcgga gaaattcaga caccttttgt gctggcccgg ggagctgaag
     4501 taatcaaata atattttcca tcgataatgc ttgttgccac aaagcacgat gatttcttaa
     4561 ggaggatgta ggctaaatga aagttttacg cgcacattta ctgatttgcg gaggcaccgg
     4621 ctgtcaagct tccgggagcc ctgcggtaaa aaaagcttta ttggaagagc ttgataaaaa
     4681 gaaattaacg gaagagatta aagttgttga aacgggatgc aacggctttt gcgccctcgg
     4741 ccccattatg gtcgtttatc ctgaaggcgt catttatata tccctgaaac ccgaggatat
     4801 ggcggaattg gtggaggaac atctggtcaa aggacgaatc gtccagagac ttctttatcg
     4861 tgaaccgaca acggatcaga ttattcccac gatgcaggac atccccttct tcgcccatca
     4921 ggaactcaga gtcctcaaga accgcggtct gattgatccg gaaaaaatcg aggaatacat
     4981 cgcccgcgat ggatacgccg gcatggccaa ggcgttaacc gaaatgaccc ccgaacagat
     5041 cgtgcaggaa attctggatt cgggattgag aggacgcgga ggcgccgggt tcccgacagg
     5101 tctgaaatgg aaatttgcag cacaatccaa gggcgacgtt aaatacgttc tttgcaatgc
     5161 cgacgaggga gaccccggag cattcatgga cagaagcgtc ctggaagccg atccgcatgc
     5221 ggttctggaa ggcatggtga tcgccgccaa agcgatcgga tcaaaacacg gatacatcta
     5281 ctgccgcgcc gagtatccgc tggccattca ccgtttgaat gtggccatcg atcaggctaa
     5341 gaaagccggc cttttgggca aagatattct gggcacggga tttgatttcg atctggaaat
     5401 ttatcagggc gccggcgcat ttgtctgcgg cgaagaaacg gctctgatga cgtctatcga
     5461 aggcaaaaga ggtatgccgc gccccagacc tcccttccct gccgttgccg gtctgtggca
     5521 gaaaccaagt attttgaaca acgtggaaac gctggccaat atcggacaga tcgtcctgcg
     5581 cggtgccaaa tggtacgcct ctatcggaac ggaaaagagc aagggaacaa aagtctttgc
     5641 gctgaccgga gatgtggcga atgtcggtct ggtggaagtt cccatgggaa caaaattggg
     5701 aactatcgtt cacgatatcg gcggcggcat tcccaaaggc aagaagttta aagccgctca
     5761 gttgggcgga ccgtccggcg gctgtattcc cattcagcat ttgaacgcac cggtcgatta
     5821 cgagaaagtt gctgaactag gcgccatcat gggctccggc ggtctgattg tcatgaatga
     5881 agacaagtgc gctgtggaca tggccagatt tttcatggat ttctgtcagg atgaatcctg
     5941 cggcaaatgt acaccctgcc gcgagggaac caaacgcatg ctggaaatcc taaccaatat
     6001 tacccagggc aagggaaagg aaggcgacat cgagcttctg gaagaaatgg cccaggttat
     6061 caaaaacgcc gctttgtgcg gtctgggaca gacggctccc aatcccgtat tgagcaccat
     6121 tcgttatttc cgcaatgaat atgaggagca tatccgcgat catcgctgcc gcgcagccgt
     6181 atgttcggcg atgttcaaat caccctgtca gcatacctgt ccgatcgaaa tggatatccc
     6241 tgcttacatc gctctgatcc gggctgaaag atttgacgat gcctacaaaa ttcttcttca
     6301 gaccaacccc ttcccctccg tttgtggaag ggtctgcgat cataaatgcc agacaaaatg
     6361 ccgtcgcggc aatctggatg aaccggtcgc cattaaattc ctgaagcgat tcattaccga
     6421 caacgccccc agacccaaag tcgaccccgt tttggtcagc aggaaagaaa aaattgccgt
     6481 tatcggcgcc ggtccagccg gtttaacggc cgcgcgcgat ctggctttac gcggctacaa
     6541 ggtgaccgtt tttgaagaac tgcccaaacc gggtggaatg ctttactggg gcattccgtc
     6601 ctatcgtctg ccgcgcaaca ttctggacgg tgaaatcgac gatatccgtg cgctcggcgt
     6661 ggacatcaaa ctcaacaccc gggtcggcaa ggatattacc ttcgccaaac tcgaaaaaga
     6721 attcgattac gtctatctgg ccaccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnntct ggtcgctcct ttgaacgtcg aaggtgcaaa aggtaaagtg acaggcatca
     6901 cctgtcaacg catgaaactg ggcgattttg attccagcgg tcgcaagaaa cccgttccgg
     6961 ttgaaggatc cgaattcacc ctgaaggtgg atgcggtgat ctcggccatc ggccaggtcc
     7021 cggatatgag tttcatcgat gccaagcgag gcgtggaaat caataaatgg gattgctttg
     7081 ctgtcggcaa ggtttacaaa tccggcacaa ccaacccccg ttatttcgcc ggcggtgacg
     7141 ctgtaaccgg tccggatacc gcgatcggag ccattgctgc gggacatcag gctgccgacg
     7201 atatcgatgc cgccattcgc aaagcgaacg gtgaaccggc ttatgaaaaa ccggcacttg
     7261 aaaaaatcga tatcccgctc attatcgatg aagaaaccgt tgaagctccg cagatggcaa
     7321 tgcctgaact gcatcacgaa acccgtaaga cgaatttcgt ggaagtggag ctaggcttca
     7381 aacgcgaaga cgctattaaa gaggcgtgcc gctgcctgcg ctgcgacgcg gctgtttaat
     7441 ttactctaaa ggtaatgcaa aaaaagaggc ttgcccttgt cgggtaggcc tcttttcttt
     7501 ggtcgatttc taaaaacaaa gactgtggaa gaagcatccg aattgttgat taagacaatt
     7561 tgaattttaa gaatttgttt gtgcttttct gcgttaagaa tctgttcaat ttatagtttg
     7621 ttcctttcaa gcctgaaaaa taccccagtg catacaacgt ttcattcttc ctgatatcga
     7681 ctctgccggc accctgcaat ttcttaatgg cccccacctg acacaacgcc ttaaaatatt
     7741 tctggagggt gatttttttt atatctaacg cctgaagaat ggcgttgtag aaatcctggt
     7801 ttgtgataaa acaggcatag ggatatctgc cttgatattt cggttcctcc agttgaaaca
     7861 aatagcggaa aggttttttg gttttggcgc ttcggctatc tttcttttca agctcattaa
     7921 gaatttcaca aaccttgtat tttataatca accgctccac cctcggatcg tcattcatcc
     7981 atttccgggc atatccgagc ttctttcccc gtaattgccg atgatagacg ttctcgatat
     8041 gttctttgta attgattaat acactgtaaa acgtcgcaat cgcctgggac tccacgtaat
     8101 caagccattc gcccttccct tcgtcctcgt catccgtatt tctttcctgc gtcgcctggt
     8161 cgatccatgc ccggaatatg ccgtcaaatt cagcctccag agaatctatt tttttatgat
     8221 cgtccgcaac gcttgatgac gtgtcttgca ccgtttgttc atcatcctta atcaggtaat
     8281 cgataaatgc ggaaaaatcg gcaaactcct tttgatagtg cattttatat ttattgtgtg
     8341 tcgaggtgac aaatttttca taatttccat tccagctctc gaataattct ttttgagttt
     8401 caaaataatg ccggagattt tggtcctgcc ggtttcccag attttctttt tttttgcggt
     8461 tattttccat gcctgcttat aaacctccaa accagtatcc gtataaaata tcgacaacgt
     8521 tttttttcaa ttgtgttgac cggtttaaaa aaaatattcc tcttgcacaa tcatctataa
     8581 ggaccttcca tcgggatgtc aattcttttg aggtaactat aagttcttga acttattcat
     8641 gacttacggt tcttcatata tatcacaaat tattaccttt tttttgacaa atttaacaat
     8701 tgagcgtata aggaatcgtt aaatttctgg caacaactgc cctgagctct gcgtgtataa
     8761 gcaatgattc attcatgcat acatgacaaa gaccggcagt aaaataacat tgtttcaaaa
     8821 tattcagaaa ggagatgaga tcatgccacg atggacgtac aaaacaaaca ttattgatct
     8881 ggatcaggtg atcccgcccg acaaagtcca atgcgacctg tcaggagact gcattgtcac
     8941 ggatcttcag ggaggaggcc ttccgcattt caaggaaatg ctcgacaagg aaggcgaagg
     9001 cgaatgggag cttgtgcagt gccagtatca cggaggcaaa ctgctgtgca tctggaaaaa
     9061 ggaaattaaa gaagcctttg ccagttaatc aatccggatt ggtctgtcat cagcccttcc
     9121 gttgagcgcg cataacgata gaggctgatc agggatgaag gtgaaaaaaa tccagtcatg
     9181 cggctttgat gcgtcgtacg ctggttattt taatgaatgg aggtagtagt gtcatggctt
     9241 ttaatgcacc agccaaaatg gtaggtcttg tcggcaatgc cggcgtttac aaatcaaaac
     9301 tttcagtatt tcaatttctt atccggggct tcatggcagg agcctacatc gcgatcggcg
     9361 gtgcgctgtg caccgtgtgt tcgaccggtg tagcgcccat ccttggcccc ggcatcggca
     9421 aattaatcgg gggtgcggtg ttccccgtcg gattgattgc catcgttctc accgggatgg
     9481 aactttttac cggagactgt atgctcgtcc ccatggccgc aatgatgaaa cggtgtacat
     9541 ggggttcggt tttcagaaac tggttctggg tctatatcgg aaacgttgtc ggttcttttt
     9601 tctatgccta tatgatggcg gttggcccgt ttgtggacgg caccgcggac ggcacgctgg
     9661 tcgtcaacgc cttcggaatg acggcgatca atatttccgt ggccaagatt ctgacgtaca
     9721 aggccgtcgg gaccgccggc ctctggtcct gcttcatgaa ggccatcggt tgtaatttcc
     9781 tggtcaacat cgcaattttg ctggccatta cggcggatga cgtgatcagc aagtttttcg
     9841 gaatctggtt cccgatcatg gccttcgttg ccaccggatt tgaacactgt gtcgccaata
     9901 tgtatttcct tcccacaggc aagttcctga cggactggta tccggccacc atcgaaaaag
     9961 tcggcggcct tttggcagcc aacggcggtt taagctgggg ggacatctgg ttctggaaca
    10021 tcattccggt taccatcggc aatattgtcg gcggatttct tttcatcggt gtggcctatt
    10081 ttctgatgtt cagaaatgaa cttccgaaag actagaggaa tcgcaattgt cactaccggc
    10141 atggataact ttttttatct gcgcggcggc cgtattcatt cactatatgc aggtgaggaa
    10201 tacccggttt ttgctctttg ccattcccgc tttgctcgct cttctcatta ttccgatgct
    10261 tttggcatgg atgagtcgga gaacgttaaa taaagcggct caagaaaacg tgtccaaggc
    10321 aaagttgtac aaaatcggca aagtgactcc ggccatgacg ggacaggttg tcagcatcaa
    10381 aggccatgtc aaaaaaatca gctttcgatg gctcaacagg ccgcattttc atgttgaaga
    10441 ctcaacaggc cggatccggg tgattatgtt taccgcaccc gatgacaata tccgagaggg
    10501 agaccccgta gacatactgg gaattgtaat caaaaatatt ttcaaacgcg gcaccccggc
    10561 catatcggcc gtcagcgtta agaagaacga atagataaca gacactttca ggaggtcgtc
    10621 atgaattcaa gagaaatcat ctttctaata ggatttttaa ttctgggcat aacgccggtt
    10681 ttcgcgcaac agcttcccgg cggagcaacg gaattgatca tgctcgcagg catgctgatt
    10741 tccacatcca cagcttttgt taaatcgtaa catccgggca gagcgaagcg gcggccggtc
    10801 gtcttcgctc tgcccctcca tgatctttcc ataaaacagg aaattgctta ccggattttg
    10861 gctaatttat ttttgttagt gttttcatca atcgacacat ccgtacatgc gccgatctca
    10921 ttgcatcttt ccatcggccg ggtttgaagc cggaatcgtg gcttcttatc tcttctaaaa
    10981 agaacggctt gcttttttgc tcaaaaagaa agtttcgagc cttatgaatc aatataattt
    11041 tctaatatta ttcataacat aaaatccttt ccatccctcc gagaaaacac gactttgtgt
    11101 ttgacaaaat aatgtattgc agctataagc ggcagtcaaa ctgctgactg ataagttttc
    11161 gaagtccaaa gagaaatttg tcccattgga agcccgttca gtcgtattac aggcagctca
    11221 atataagaag aagttataat aaatttacta aagtgaggta aggaatttat ggcaaatgaa
    11281 aatttagaaa gtaagaggtg gttgattgcc ggcgcggctg ttatcattca gttgtgtctc
    11341 ggcaccgtgt acgcatggtc ggttttcaag aatcctttga tgcagatgca tggatgggaa
    11401 ggcaaatctg ttcagtacac gtttatgctg ttgatgctga tcatcggatt agcggctgct
    11461 ttcggcggta ccttggttga taaaaaggga ccgcgttttg tcgccaccat cggcggcatt
    11521 ttattcggta tcggcaccct ggtggccggg tacgcagatc aggttgggaa catttatttg
    11581 ctttacctgg gtttcggcgt tatcgccgct ctgggtaacg gtttcggtta cgttacgccg
    11641 attgccacgc tcattcgctg gttcccggac aaacgcggtc tggtaaccgg tcttgccgtg
    11701 atgggtttcg gcgccggtgc tttcttcatg gggaaaattg ctcccgtgat gatcaaatcc
    11761 ttccagcaga tcgaccccgc aggaaccatg atcgcttccg gcgttgccaa cacctggtat
    11821 atctggggta tcatattcct gattcttgtt acaggttccg ctcagttatt taaaaatcct
    11881 ccggccggtt ggctgcccaa aggattcaca cctgccgcca caaccgtgtc cgcggcggat
    11941 tcctttacct tcggagaagc tgtgaaaacc cgtcagtggt ggatgctctg ggccatgctg
    12001 tgcctcaacg tttccgcagg gcttggcttg atttcccagc actctcctct ggcacaggat
    12061 atttacaaaa aaacttatgg gttaacggga gacctgacgc ctgaacaagt tgcgatcgtt
    12121 gccgccgccg gtggcgctgt tgtcgcttac gccgccatct tcaacgggct gggccgcttg
    12181 ttctgggcaa aaatttccga taatatcggc cgcaaaatgg tatttcttat tatgtttgcc
    12241 acccaggccg ttttgtatgt gctcgttgcc aaaggttttg ttgcaacata ctggcttttt
    12301 gtcatcgctt cctgttatct tttggcctgt tacggcggcg gattcgccac catgcctgct
    12361 tttgccgctg acgctttcgg tcccgcttac atcggtaaag tctacgggtt tatgttgacg
    12421 gcatggagca ctgccggtat catcggccct tatgtttttg aagtattcaa acctcaggcg
    12481 ctcacgattg ccgccggctt attgaccctc ggttttgtca tcgcactgat ttacacccct
    12541 ccgaagggca aaaaagcctg atacgtctta tctcaaaagc cccgtccgta ttcaacggac
    12601 ggggcttttt cacaccttgc cgcacatttg cgtctatcat tattttaatt ctgttgacat
    12661 ttaacagggt attgtttatt ccattacaag cttcgcttgt ccgctgtctt ctttcgcctg
    12721 ggcctcaatg tatttccgaa ttacgctctc atttatcccg accgttgaaa caaagtagcc
    12781 tatcgaccaa ataccttcca ctccccaata gactttgtcg agaaatttaa atcttttcct
    12841 catcttctgt ccggtgttgg ctttgatttt acccacaacc tcactcactg agtatttcgg
    12901 tggtattgac gccaaaaaat gtacgtgatc caaatccgtg ttcacttcca atatttctat
    12961 gtctggatga tatttgctca cttccaatat gagcttttgc aaatattcgc caacaccttc
    13021 cttgaatatt tttcggcggt atttggtcga aaccactata tgatatctcg tataatatac
    13081 cgcatgggct tgtttttgac attccattca tatagtatat acgaagacag cggatacttc
    13141 catccacagg ctcacgcctg tggtattacg aatgtggaat aaaattttaa ttttcgcatg
    13201 ataaactatt atattaattc tacggaagat tatcacaaaa atgaactcaa ctcgcttggg
    13261 tgggaattaa ctgtctgcaa tgcgcttgat cctatggaca gtccctgccg aaggcttatt
    13321 gcctcacaga catcattcgg ggaaaattta tatcgttttc tggaagaaat catacccgtc
    13381 gacacaatcc gtacgattct ggaaataggc ggcggcatgg gttatctcat gcgtgatttc
    13441 ttgaaattca atccgcgcct gaaggcaaca atgattgata tctctcccta cctgctgggt
    13501 tgtcagaaag acctgttaca ctcttttgaa gtcgattttc ttctggccga tatcctcacg
    13561 gtgccaccga cgacattatc ctctttcgac atggccgtga tgaatgaaaa tctgggcgat
    13621 cttccgacac tggtgaccgg atcaaaagac gacatctacc atggccctgc cgccggagtt
    13681 atcgaaaaaa tagaagcctt caatataaaa tataacctgc agctgggagc caatcaaaac
    13741 gaacccgtca acatcggcgc cctggaaatg gtggaaaaac tctgtctggc cgggatcaaa
    13801 tatatctatc tgagcgagca cagctgtgaa gccgccgttc cggagaaaat gaaaaattat
    13861 ctcttttttc aaacacccgg cattccggaa atgatttccc tgaaaggaca ccgcgaattc
    13921 accatcaaat tttcctattt gcagaaaatt gcggaaatgc taaattacac ctcgcggcgc
    13981 ggaccgttcg ccgattttct ccctctggat ttcaatgaca gggtaaaaac ggcattgcga
    14041 atgttgacgc cgcaaagcga caaccaggaa attatcaggc agtttgttta tgatcttttt
    14101 aaatatgagt atttaattct tattcgagga gaccacaaat gaagaagacc gtcatgatta
    14161 tttttccggc attatttttc ttgctgtctg tttccgtcag tccctgccac gcttttcggt
    14221 gcggaaacgg actcgtcacg accggagaca ccaaaaacaa ggttcagatg acctgcggaa
    14281 agccctccgc caaggaaaaa cgatgtctga atagctggac cggcaaaaag gccaaatgcg
    14341 ataaaaaagt cgaggtatgg tactacaact gcggcgacaa cgacttcgtt tacgctctga
    14401 ttttcgaagg caatacatta accggagaaa actctgaagg ccggggccat ggaccttcag
    14461 aatgccgggg acagtgaaca tagcactggt ggctccgatc ctgatagccg gaatttaaaa
    14521 gagctgagcg ttgtttcgct tcaagatgtt tttcgaatgt acgccgctgt taagagaaat
    14581 ccgccgggaa agcgccatga tcgtaacgct tttataatga ataatcattg acttgccttc
    14641 atgatttctt ctatactgtc tttgtggaag caagtccttc tccctgatct ccgaaggata
    14701 acgcatgtac aaatgcagcc ggcgtggcgt caggtcccgc atacttttga tccttaagga
    14761 tatctcaaaa ggataaacct atgacaatgg acatgttgat cgtattcggg atcatcctgc
    14821 tggccgccct gctgtttgcc tcggaaaaac ttcgcgtgga tcttacggca atcgtcatca
    14881 tggttcttct cattctaacc ggcatcattt caccctccga aggcatatcc ggcttcagca
    14941 atgtggccac catcacggtt gcctccatgt tcatcctcag cgcggccctt cagaaaaccg
    15001 gttcggtaaa ttttctcgga acgctttctt ccaaaatatt cagatttaat ttctgggtgg
    15061 gattgatcgg cacgatgatc tttgtaggcg ccgtatcggc gttcatcaat aatacgccgg
    15121 ttgtagcggt ttttattccc attatgctgg gagttgcggc agacaatcaa atgagccctt
    15181 cacggcttct gatgccgatt tctttcgcgt ccatgttcgg cggcgtgtgc accctgatcg
    15241 gcacatcgac aaatatcatt gtgagttccg tcgccgttca gcatggatta ccagccctgg
    15301 gcatgtttga attcaccaag atgggactgg ttttttttgc cgccggtctg gtctatatgg
    15361 cggttgtggg ggtccggctg atcccgttga agaaaacgga aacggacctg acgaaaaaat
    15421 atcagatgaa tgattatctg acggatatcg tgcttctcgg caacgccaaa tccatcggca
    15481 aacgcgtcgc cgattcaccg ctgatcaaag agctggacat cgacatcctt gaagtcattc
    15541 gaaacggacg gcgtcttcac gtccccgtgt ccgatatcgt tctgatggaa aacgatcttt
    15601 tgcgtgtgcg ttgcgatatc aagcagttgc aaaagctcaa agagcggatg ggcattaaaa
    15661 tgaaatcgga ctgtgaactc aacgacgaaa atttccgctg cgaagccctc agcctgactg
    15721 aagttgtggt tgctccgaat tcacgtcttg agggaaaaac catcaaatcc tcgtatttcc
    15781 gtaatgtctt tcacgcaacg gcactggcgc tgaggcacag gggacaactg ttcaacaccg
    15841 gatttaccga tacgattctc agagcgggcg atgccattct ggtggaaacg cgcaaagaaa
    15901 attatgaggc gctgaaaaac agcaacgatt tcgtcattgt ctcggatatt gagacgcagc
    15961 aataccgtaa aagcaaaatc attccagcac tgctcgtcat tgccggcgtg attgtaacgg
    16021 ccagcctggg cattattccc attgtggtcg gggcgatgat tggcgctatc ctgctggttc
    16081 tgtccggctg catcaccctt gaggaagctt acgatgcaat cgactggcag gttatttttc
    16141 tgctcggcgg agttctatcg ctgggtatcg cgctggagaa aacaggagcg gccatgtatc
    16201 tttccaataa tttgatttat gttttgggag atctgggacc tgtcgccatt gtagcagcgc
    16261 tttatctgct cacctcgatt ttgacggaat taatgtcgaa caatgccaca gccatattgc
    16321 ttgcgcccat tgccattgct gcggccaacg ggatcggggt tagccccacg ccctttctga
    16381 tggcgatcgc cttcgcggcc tcggcaagtt tcatgacgcc ggtaggatat cagaccaaca
    16441 cgatgatcta cggcgtcggg cagtatcgtt tttccgactt cataaaggtg ggaacgccgc
    16501 ttaacatcat gttctggatt ctggcgacgc tcttcatccc tgtatttttt cctttttagg
    16561 aggtgacatg gacggcatca cggctttgat aaatcagatt ttgaatgtat tccgtattcc
    16621 tttatttcat atgggtgagg ctccgtttac gttatggacg tttatttatc tcgttgcggc
    16681 tacgatgata ttaacgctgg cggcgtctgt gttaaataaa gtcattgtta cccgtctcct
    16741 gacaaaaagc aacatcgaac ggggcgttcg tatcgccatc ggatcgatcg ttaaatatat
    16801 tgtactggtt gtcggattta tcatcattct gcagaccgcc gggatcaatc tcagcagcct
    16861 caccattctg atgggagctt taggcgtcgg tatcggtttc ggtttacaga acatcaccaa
    16921 caatttcgtc agcggcatga ttattctgct ggaacgtccg atcaaggtcg gcgatcgcat
    16981 tgaagtggcc aatgtttccg gagatgtggt ggatatttcc atgagggcga cgacgatcgt
    17041 caccaatgat aatatttcca tcatcgttcc taattcccag tttatttccg aaaccgtgat
    17101 caactggagt catacggatc ggagtgtccg ctttaatttt ccgatccatg tttcataccg
    17161 ggaagaccct caggcggtca gaaaactcct tctggacatc gcattgaata accccggcgt
    17221 gctcaaagat cccccgccgg atgttctttt ttccgaattc gccgacagcg ccatgagttt
    17281 caatctgcgc gtctggacaa gggagtatat caaccgcccc ggcgtgctca aaagccagct
    17341 ctactatcaa atcgccgacc gcttccggga aaaaggcatc gagattccct tcccgcagca
    17401 ggatatccac ataaaagaaa cagcgcggca atcctgaagg cggcacaatc acccaaaaat
    17461 gatacctgcc tgtcaagtcc tgattgtcac cggatgcacc ggatcgttat tttataattg
    17521 aacatccccc cgattttgaa cgcatccctg tcttgatgaa cggggcaaac atatgcttta
    17581 ctgtttttcc attttgcttt tttgtgttac atgataaaca ggaattgacg atatgagacc
    17641 tgtccggatc attttattat tgatggtaat gttatggctg atgacaaccg gctgttctga
    17701 aaaattgccc attttatact ctccgttgaa agatgattat cagctttccc ccggatttac
    17761 ggatcgtgaa cagggggaaa aacaactgga tgaatttttt gaggaggatc aaagacttta
    17821 cagaaatctt tcccggttga cgaccgtaat agacgatgca gaggacgatc cgggcgatga
    17881 agcagaaatt cactcctatc cggaagcgca taacggaatc ctcgaagggt tgaccgccac
    17941 agaatggttt actgcaggcc gtcaggctgc ttcggacagg agatgggacg acgccttaaa
    18001 cgctttcaac aaagccattg ccttaaatcc ggaaaatgag gaaatctact tccatcgcgg
    18061 taatgtgtat gatgccctcg gcaataacaa aaaagccgtc tccaatttta ataaagccat
    18121 taaactgaac ccgctgtatg tcgatgccta tctaagccgc gccttttcct ataataatct
    18181 tgctcaggcc aatcaggcgc ttgccgacat aaaaaaagcc gccagactgg gcagtctgca
    18241 tgcgcagaaa tttttaaaaa aaagcaacct ggcatggtaa gatttgtcca tgcggacatg
    18301 tgattttacg agttgtccgg attttgcaaa cggcgtttga ataacaacgg cacggctcat
    18361 tcatccccgc acgttattat tgaatttact tgacacacat tttgaatata attatacttt
    18421 cgaccgaaaa agctaaacgc ctgtgaaaaa ttaaacgaag ggaaatgctt ccgataattt
    18481 tttgtgaaat tggaacaggt gatttgtttt tcaagcaata tatttttata aataaattcg
    18541 aatcaaagga gaacattatg agaaaaacca ttatcgcaac ggtgatgatg atgctatcgg
    18601 ctttttttat aaccacagca caggccgata tccgtcccgg ttcttatacc gtcacaccct
    18661 tcatcggggg attcatcttc gaaggcaatg aatactaccg gggatttgac aatcagaaaa
    18721 acgggagaaa tactttagac gacacctatg ccgtcggnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnntaa aatattctgt aacggataaa atagacctgc gcgccgatgt
    18901 ccggcatgtt cttccgctga atgaaagata caacgatctt ctctacagtt ttggcgttac
    18961 cttctcgttc ggcggagccg aaaaaggcaa aaagctgctt gatatcaaat tcgatttcga
    19021 caaaacagat ataaaggcgg cttatcataa agacattaat gccgtgacaa aattgatgca
    19081 gaaatatccc gacctcaata tcgtgaccga gggacatacg gataacatcg gcacgcctga
    19141 atacaacaaa aacctttcca ataaacgtgc cgatgccgtt aaaaaataca tggtcgaaaa
    19201 aggcggtgtt gctgccggac ggattgatac caaggggctg ggagaaggat cccccgccgc
    19261 atccaatgat acagaggcag gacgcgctga aaatcgtcgc gtggaagcgg ccgtggacta
    19321 caccggcaaa aagtagatca atggcttgaa ccattatcaa aaagcccctt caaatctttg
    19381 gaggggcttt tattttaggc tggaggggga aggcagctcc ggaggacatc ctgcgaaaaa
    19441 gggagcgctg tcatcctttt ttccggtctg tcagtctttt tttactgact ctctttgatc
    19501 agcattgttg cgggatcgag cagtaaatcg ttttgggctg aatttttcgg ttggcctttg
    19561 acagacgtga tatacacctc atcccctttt gtcgcaagct gaacaatgac ttcaaagaga
    19621 tccgcgatat gcagaaagga aagcggcagg ccgttttcct gcggcggttc atgacgatgg
    19681 gtatgcacgg taaaccataa gcccatggag tatttaccgg ccagttcctt caactgcacc
    19741 agctgaccgc gccccgcctc gtcgaatctg aatccgtcga taatgacggt gtgcggcttg
    19801 aaaatattct gttccatcag gtcggtcaga cgctcttcca gtttcggcgc gctgaatcct
    19861 tccaccttga acgtcatgat gaaacggtag ggctgaattt tatcccagta ttcattcacc
    19921 tcggcaatgc cgaatttctc ggcaatgtca tgaaaaattt cctgatacca caggtcgacc
    19981 ttgctgacgg cgtcattcag gctgacatgc agaaccgaat gctcccgcag catcatattc
    20041 aaggccaatt gcacgagcaa tgccgttttg ccgacgccgg catatgccag cacggcccca
    20101 aaaccgcccg tggacagaat atcctcattg tcataaccga gtttgacaag aggattccgg
    20161 taaatcattt cttttttgat catcacaatg tccttatacg ttaaatgaat aatatttttt
    20221 tcccttttca gggcaacgct gattgtctgc atgttcggcc gattactatg cggctgtttt
    20281 tttgttctga gccgcttcca gggcaatttt ttccgcaatc gactgcggaa cctgccggta
    20341 aagagcaaat tccatcgtaa actgggcttt cccctgtgtt gccgaccgta aaaccgtaga
    20401 aaatccgaac atttccgcca aaggcacctg tgcttcaatc acgcacatca catcttcatc
    20461 ctgagaaccg atgatcatgc cgcggcgctg attcaaaagc cccatgaccg atccctgaaa
    20521 ttccgccggc gtttccacaa cgactttcat gatcggctca tgaataaccg gtttcgcccg
    20581 gagatatgct tccttgaaag caccgcgcgc cgccgcctgg aacgccatat ccgaagaatc
    20641 caccgcgtgg gaagcgccgt cattgatgac caccttcacg ccggtcaccg gaaattccag
    20701 tttcggcccc ttggccatgc tctgtttaaa ccctttttca caggcagcaa tatattggga
    20761 aggaatcgcg ccgccgaaga ttttattttc aaaaacaaaa tccgtatcgg caatcggttc
    20821 cagatagccg gctacccgtc cgaactggcc ggcgccgccg gtctgttttt tatgcgtata
    20881 attgaaatcg gcgcgctgcg tgatggtttc acggtacgcc acacggggat tgcccgtgga
    20941 aacatcagcg ccgtattcgc gccgcattct ttccacgtaa atatccaggt gcagttcgcc
    21001 catgccctca ataatcgtgt cgccggtttc atgatcgaca tacgttttga atgtcggatc
    21061 ttccttggca aatcggttca gggccttggc catcgccacc tgcgatttgt tgtctttggg
    21121 aacaatcgcc agagaaatga ccggtttggg aacatacatc gaaatcatgg ttaaattgac
    21181 atccggcgat gtaaaggtat cgccggaaga acattcgatg ccgaatagtg cgccgatata
    21241 acccgctctt aaagattcga catcttccat ctgatcagca tgcatgcgca ccagacgtcc
    21301 taccttcacc tttttgccgg tgcggacatt cacgatggtc gacccttttg ccaacgttcc
    21361 ctgataaacc cggatatacg tcaactggcc gtattgaccg tcttccagtt tgaagaccag
    21421 agcgacaatc ggtttttccg tatcattgtt caggacgacc gtttcttcgt ttttatccat
    21481 atctatcgct acgttttcta tgtccgacgg acagggcaga agataggtaa cgccatcgag
    21541 catcggctgc acgcctttgt tcttgtaggc cgatcccagg taaaccggtg tgatctctct
    21601 ttgcagcgtc cccttgcgta aagcggaata gatcaagtcg gcgctgactt ctttttcttc
    21661 aagaatcgct tccgtcaatt cgtcggaaaa catcgaagcc gcatcaatga gttcttctcg
    21721 tttcgctttg gcctcttcca gaagatgaga cggaatttcc gcctcgcgaa ccgtttcccc
    21781 gttatcgccg tcgaaataaa gcgctttcat actgaccaga tcaacgacgc cctggacatc
    21841 attttccagg ccgatgggaa tctgcatcgc cacagcattg tgtcccagct tcgatttcaa
    21901 ctggctgatg acacggaagg gattggcgcc gctgcggtcg catttattaa tgaacgcaat
    21961 gcagggaacc ctgtaccgtt tcatctgacg atcaacagta atggactgcg actgaacgcc
    22021 tcccacagcg cacaaaacca gaatagcgcc gtccagaacg cgcagagacc gctccacctc
    22081 gatggtgaaa tcgacgtgac ccggcgtatc gatgatattg acttcgtggc ctttccattc
    22141 acaatacgtt gctgccgaag caatggtaat gccgcgctcc ttttcaaggt ccatggaatc
    22201 cattgtggcg ccgacgccgt ctttgccttt gacatcgtga atggcatgga ttcttttcgt
    22261 gtaataaagg attctctcgg tcagcgtcgt cttgcccgaa tcgatatggg cgctgatgcc
    22321 gatattacga attttctgat cgtcaatttt cattatcttt tccttcttta taacttctat
    22381 aattccattc gcttcactca tggattgttc gacttatacg tttcagtaca tttcttttct
    22441 gaaacgtgag tctcacaaaa aaaccccaca cttgtaagta tctctgcaag atggggcatc
    22501 cgccataaac aagcatttgt gaacttggct tcatacatat tgcaacgcaa tatgtcaagc
    22561 agttatttaa catatttaga acaataagaa tgcttgtgta ttattgttga acatgataat
    22621 tatctttcat gtttaattaa ttgaattata aaggagtttt tgatgtacga gcagatattg
    22681 ccaaaccttt accggattat catccccctg cccaacagtc cgcttaaaga tctgaattct
    22741 tacgtcatta aagccgataa ccggaatctg attatcgata ccggattcaa ccgcggtgtc
    22801 tgttatgagg ccatggaaaa tggcctgcaa gccctggaga ttgatttatc cagaacggat
    22861 ttcatgctca cccatatgca ctccgaccac acgggactgg tgtcccggct gtttaccgag
    22921 accagcaaag tctatttcag caggatcgat tcagaagtat tcaacgagaa tatcgactgg
    22981 caggccatgc tggattacgc attgcataac ggtttcgaac tggaggaact catccgggcc
    23041 gcagagagtc atccgggatt taaatacaga ccgaggaaaa ttcccgactt caccatgctg
    23101 gacgacggca gc
//
LOCUS       DAOSME010000037        21780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_2998_length_21780_cov_24.100944, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000037 DAOSME010000000
VERSION     DAOSME010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21780
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2998_length_21780_cov_24.100944"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /gene="surE"
                     /locus_tag="PLG98_06900"
     CDS             complement(<1..377)
                     /gene="surE"
                     /locus_tag="PLG98_06900"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOE33020.1"
                     /translation="MRFLLTNDDGIYAKGLSALYKELSKEADCLIVAPETEQSAVGHA
                     ITISRPLMVRRATKNNQFLGYAVCGTPADCVKLGIKELSDHPVDLVVSGINRGGNAGI
                     NVLYSGTVSAATEAAIMGFPSMAV"
     gene            complement(441..1025)
                     /locus_tag="PLG98_06905"
     CDS             complement(441..1025)
                     /locus_tag="PLG98_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE33021.1"
                     /translation="MNVLSFRKKYFYLPVLVFLISGFIPGVFAAGKERQANQCKTVIL
                     ANEEFLPALLNAIDGAQDEIIMSIFSFKAGVHKNSYPDRIAGHLAKAVKRGVKVFVVL
                     ENTGDSSDALNIQNRKAGKFLAGKGIRVYFDSPRKTTHTKLTVIDQRLIFLGSHNLTQ
                     SALKYNNEISVLLEGRDLALNARNYILGIIKEGT"
     gene            complement(1134..3056)
                     /gene="dnaK"
                     /locus_tag="PLG98_06910"
     CDS             complement(1134..3056)
                     /gene="dnaK"
                     /locus_tag="PLG98_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOE33022.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGDPVVIANQEGNRTTPSIVAISE
                     SGERLVGQVAKRQAITNSENTVYAVKRLIGRKYTSKEVQYDLKIIPYKITEASNGDAQ
                     VTVRGKNYSPAEISAMILTKMKQTAEDYLGEKIKDAVITVPAYFNDSQRQATKDAGKI
                     AGLNVMRIINEPTAAALAYGLDKKKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGEDFDLRVMDFLVAEFKKDQGIDLRNDKMALQRLKEAAEKAKMELSTTMETDI
                     NLPFITADASGPKHLNIKLTRAKLESLVEELINQVVGPCQTALKDAGMSPKDIDEVIL
                     VGGMTRMPRVQQKVKEIFNKEPHRGVNPDEVVAIGAAIQGGVLSGDVKDVLLLDVTPL
                     SLGIETLGGVMTRLIEKNTTIPTKKSQIFSTAADNQPAVSIHVLQGERQMAADNRTLG
                     RFELVGIPPAPRGVPQIEVAFDIDANGIVHVNAKDLGTGKEQSIKITASSGLSDADIE
                     KLVKDAEMHAEEDKRRKELIETRNQADSMVYSVEKNIKEFGDKIDSAEKTKIEDAIAR
                     TKKALEGDDVEAIKKAQDDLMNVSHKLAEAMYAKSAGPQGGPGADGGAQQQSGDQQAS
                     GKKNDDVVDADFEEVK"
     gene            complement(3067..3672)
                     /gene="grpE"
                     /locus_tag="PLG98_06915"
     CDS             complement(3067..3672)
                     /gene="grpE"
                     /locus_tag="PLG98_06915"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOE33023.1"
                     /translation="MKSKKEQSEHSAKGFQAGEHKDKDAAEDRNKEVEALKQKLEEKE
                     NEATANYDRYLRAVAEFDNYKKRALKDKADTIKYANEEIIKDILPFMDSLDRALEQEA
                     GDVQTFREGIALIQEQLLCCLKKYGVERIDAAGQDFDPNFHEALMQMESDQHEDNKVV
                     SEMEKGYLLNGRLVRPARVCVCKKTKKQEDDMIEIMEDTGE"
     gene            complement(3701..4285)
                     /locus_tag="PLG98_06920"
     CDS             complement(3701..4285)
                     /locus_tag="PLG98_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOE33024.1"
                     /translation="MEDYIIKIFKESNRVKDNFLNENLGKLANVIEVITSALKAGNKI
                     LLFGNGGSAADAQHLAAEFVNRFIIERPPLPAIALTTDTSVITSIGNDYDFSEIFSKQ
                     VRAIGQPGDIAWGISTSGNSANVLKGLEMAKKMGLVTVAFTGKDGGDIAKIVDFSINV
                     SSNSTARIQETHITAGHAICELVDIKLFQKPDFQ"
     gene            4557..4997
                     /locus_tag="PLG98_06925"
     CDS             4557..4997
                     /locus_tag="PLG98_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE33025.1"
                     /translation="MFGPNLWRMKRFADSVPELIDLQREINRLFSSVGQAAGADYPAI
                     NVWEKEDSLVVTTELPGMDPDDINISVTGSMLTISGKSKGDPLKAGETYLRQERELGN
                     FQRNVQLPFQVDSPAVEAKYEKGVLNITLPRQKEDLPRKIQITA"
     gene            5015..5407
                     /locus_tag="PLG98_06930"
     CDS             5015..5407
                     /locus_tag="PLG98_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE33026.1"
                     /translation="MSDKDLQKTENTAATERIRNVKTFVPRVDIYETKDALFLIADMP
                     GIDEQTVDVELEKNILTISGRVENGKVKDYRLVFSEYEVGDYERTFTLSDEIDRDKIK
                     ANVKHGVLRLELPKAEEVKPKKIKINAA"
     gene            5463..5957
                     /locus_tag="PLG98_06935"
     CDS             5463..5957
                     /locus_tag="PLG98_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE33027.1"
                     /translation="MMIKNLLPDVVRRTRADLDQPFYSLQKEMNNLFDSFFRGIEPAP
                     RGFAGYFSEAFSPSIDVKENDKEFIIKAELPGVEEKDIDVTVTGDSVTIRGEKKEEKE
                     DKDKNYYYMERSYGSFCRVIPLQTEIESGKAEARFKNGILDIKIPKSQSAKAKGTKVS
                     IKSG"
     gene            complement(6023..6874)
                     /locus_tag="PLG98_06940"
     CDS             complement(6023..6874)
                     /locus_tag="PLG98_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOE33028.1"
                     /translation="MVGTLRKSLKTIEDYKSANPHYTELLDIMADILILREEYLKNMS
                     RPIFHVDENLIAQKMTGGLPLIDLTDKEFDLKRPRDYFYALIQAAEKRIPELEQSISD
                     IVDDPDFDWEKSVRASFDCKVEEADARTAEADREEENNIDLIDLFLEESLRPELEIIA
                     KKYGEMVAKSDWTEGYCPICGKEPKIGEIRKDEEGKRYLFCHQCGFKWYFYRIKCPFC
                     GNDEQQSLAYFEVEGEERYRVDVCNKCWRYIKTVELPKFSDEPNMDVEDIATLHLDMI
                     AYDEGYN"
     gene            7316..7759
                     /locus_tag="PLG98_06945"
     CDS             7316..7759
                     /locus_tag="PLG98_06945"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOE33029.1"
                     /translation="MFRYEFVTQIEIDATAERVWNVLTDLDSFPQWNPMIRWAKGKVC
                     SGERLTVRFEPKGTRGYTFTPKLLIVEPNRQLRWLGYPRIPAFMDTEHYWLIEQITGE
                     KSRLLHGAIIYGLLTPVSRIIFENTSRGPFEQMNHAHKKRAESRE"
     gene            complement(7773..8150)
                     /locus_tag="PLG98_06950"
     CDS             complement(7773..8150)
                     /locus_tag="PLG98_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE33030.1"
                     /translation="MKKFSVIDRWRIPTYDNPNHVKTADLFFNSKKCKQCGTCVKICP
                     GGCIVTDKISKKDLMQDMKKAVKSGVPRVDVLKTGATLCIACFDCGAACPHGAISIKR
                     NFDPKYFYKRLTQLSDMRLPRKY"
     gene            complement(8152..8907)
                     /locus_tag="PLG98_06955"
     CDS             complement(8152..8907)
                     /locus_tag="PLG98_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE33031.1"
                     /translation="MSSKDDVLSPLGKYEEGMAWNPVEKVIMERRSIRAFKKEPLPDR
                     MIERILEAGRFAPSAGNSQPWKFIVVNSPEILAEMEKDALKIIKFFMFFIDYTRGNIL
                     RRLYILSWVKWIIRILKNKLHPVPFGLMSQIAQGRAPVFHGAPTLILLAEDRRGVASP
                     PTDIGICGQNMVLTAHSLGAGSCWIGLIQVLMYLPKWRKKFGIKYPYKLNECIALGWP
                     NPRVDGMVPREVQLVQWYERGMNDAPRITKQGE"
     gene            complement(8961..10676)
                     /locus_tag="PLG98_06960"
     CDS             complement(8961..10676)
                     /locus_tag="PLG98_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE33032.1"
                     /translation="MYLLANLKIGSSMIKSLLTFFKDEIRRGTFRANFSSLMKTQEIT
                     EDMSWAELSEEKARQYGDRTFLIYEDREFSFRQMDENANRVANFLQSLGAGKAKGVAI
                     IMANCPEYLDVYIGSQKLGMYSIPINTSLRGDSLLYILNHSDAEFIVIDEEFLDAYNK
                     IADRLERKKTVIVNRSVPPGASGLPHGLLNLADAYKQSPRKPNVPYDKNDVCFILYTS
                     GTTGLPKGVMYRYGKTTLKLLSIPAYALYKRSDILYTCLPLFHGNALWLCVTQGMHRG
                     CRVVLGRKFSASRFWDDMRKYKITAFNTIGAMIPILMKQPPRDNDSVNSVRFTLSAAC
                     PVDEWGKFERRFGIKIYEAYGSVDGGGKSIMNLGNAPVGSIGKPAPNTVYRLVDNEGL
                     DVPDGTPGQLIFESKGKKKSVEYFKNEKASNDKLRNGWIYTGDLVKRDKNGYLYFVGR
                     NAEFMRIKGENVSAYEVEHTIQKHPSVLEAAVYAVPSELAEDEIMASVSLVEGQTLKE
                     ADLIAFLKDDLAKFAVPRFVRIVREFPKTETQRIIKKELEKIGVVAGTYDAQKNQYVG
                     NTKIA"
     gene            complement(10740..11861)
                     /locus_tag="PLG98_06965"
     CDS             complement(10740..11861)
                     /locus_tag="PLG98_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE33033.1"
                     /translation="MLTTVKCELKSFEDLAHNFAAKELAANREANDRYPFGPLFDSVI
                     DKAHDVGLLGATLPEECGGIGQDIGALCIILDHISAADASLAGIIFTNALAQEIVLAA
                     GDKNLLTALMEKAADARAALIAFPSFSNPAENGVSLEAVKSKDTYLLSGAMEYIVLGG
                     LAGQALVPVKIKGEEGFSFFLIRPADKGVTKSEPVFSLGLHACPAVDLTLSAVEGQLI
                     GQAGEGIKYFNQASDRMHAAAAAIQCGIMRGSFQEAFDYSRQRCQGGREIINWSGLRM
                     LLAQMAVQVKIAEMVVSEAAMAVENQEKQWGLCTRAAALHLSELACKLTADGIQCLGG
                     NGYMKDYGQEKRFRDAKHVQALLGMAPMRKLSLIRDMLS"
     gene            complement(11873..13381)
                     /locus_tag="PLG98_06970"
     CDS             complement(11873..13381)
                     /locus_tag="PLG98_06970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE33034.1"
                     /translation="MIKPKEVKSLLDREPRFEDSFCSVQAIPKIPKIQLKETRTLVSH
                     ARRFNDEVVRPLALKTDLKTFQDHDYLPWEIIEKANEWGFYTMFIPKIFGGQGWSLPS
                     HAYVIEELSSACVGIANVAFVHYLGFMTLLASWNLPLINRVCREVAEGEKTGKPCLIS
                     LAITEPGAGTDVEEVELVDRGKVTCFAEKVKGGYIVNGSKIFISMGHVSTWHALIAYT
                     DLKKPSANTLMLAVRTGMKGFTFGKHEDKMGQRACCASELIFQDCFIPDEYVLGDSRD
                     FPNSQIRETSDLVNQIIHYVVEVTRASVGAFGTGVARGAYEHALKFAGETEVDGKLLI
                     NHEWAQIMLAEMYKNVIAGRLAYNEANYSNSLKGGIFNFLQVKPLYYYLKYVPQIVID
                     TFIVPFYRFKMTDWAIRQIFVEGQSMEAQKRCSGWSSIAKFFGTDMGLKNSQMALELM
                     GQAGLRHDRGAEKFLRDSKLLQIYEGTNQLNRLNLFNCLIARCVPQARVFEE"
     gene            complement(13528..14937)
                     /locus_tag="PLG98_06975"
     CDS             complement(13528..14937)
                     /locus_tag="PLG98_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOE33035.1"
                     /translation="MRDVYIIGVDTIKFGKHMDKSIKDLAITTATGCLKDAGLDKKDI
                     QALWFANAGWGEGGQMTIRGQVALKGMGIEGIPITNVENACAGGSTALHHAWYGVAGG
                     LYDITMAVGAEKLYFSNKNLVFFGFLGGIDIENVLNIAKTLSVFTLTPEEKVKVEEFK
                     QRYQAVKKAANNGGVKKSKAKESKWKAYRDKFTAFIVLGEKIGYDTVRQLARMSSGDH
                     SPFMDVYGFAARQHMKKYGSTAEQMAMIASKNHFNSTLNPNAQYRFDVPVEKVLSDRI
                     VSWPLTRSMCAPIGDGAASAILCSEDMVKKLGLTHQAVKIRASILGSAIRTSFDSDIP
                     EIGERLSKQAYEKAGVGPQDINVAEVHDASAFGEVVQAENLGFCPKGEGGIFAEKGET
                     SLKGRIPINPSGGLISRGHPIGASGLAQVHELVTQLRGNAGKRQVNNARLALAENGGG
                     ALGTEEAAMCIHILEAPSS"
     gene            complement(15063..15974)
                     /locus_tag="PLG98_06980"
     CDS             complement(15063..15974)
                     /locus_tag="PLG98_06980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE33036.1"
                     /translation="MKKTTMGANSKINSSANAILVTGASSGIGWATSLELAQKGWRVF
                     AAVRKEADAKKLRDASSGKITTVIMDIVDYESVKRGASEIEKALGGAGLDALFNNAGI
                     SVQGPLEILPIELFEQQIRVNVFGHIFVTQTFLPLLRKAQGRIVFTSSESGRVTLPLM
                     APYSSSKFALEAVASALRIELRPWKIRVSSVELQTIKTPMWEKIDATTEKLFASVPKQ
                     ARDLYKDELRTLSVFPKWQAEMGISMKKAVRVIIRALSVKRPKARYLVGWEARFLVYC
                     HNVTPIWLMDWFSSKIMILLGKFIKPK"
     gene            complement(15989..17026)
                     /locus_tag="PLG98_06985"
     CDS             complement(15989..17026)
                     /locus_tag="PLG98_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE33037.1"
                     /translation="MKMDYKYLKIQKENHSLWVEMKNPPVNFLTVAMLEELFDLVKQV
                     SRDPSIRVFILTGGIEDIYIMHFSIPELFTLSKDNKKIRLNLFIKTRLTAAILKHFTT
                     CTNGLMDGFGWYEKLVLKFAKVLSGYSSSLYLWFIMQRLYMAIERLNKITIAAINGNC
                     NGGGTELSACFDFRFAVGDQGFTIGQPEVLINIVPGGGSTQRLPRLIGKAKALELMLR
                     GNQWDAEEAHRVGLLTGFFNKAEFKTKVQEFADLMSKRPMTAVDAIKKCIHNGMETTI
                     RHGLSIEMEQSIRCLDTDDTMNAMQAYIRYLDQHINAMDPQKMTTEEINQVVQDTVKH
                     MEEGKLYKFKH"
     gene            17282..18007
                     /locus_tag="PLG98_06990"
     CDS             17282..18007
                     /locus_tag="PLG98_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE33038.1"
                     /translation="MNILVRQIFSRQLKPGDKLPSERQLSKDMDVDRASLRIALKHLE
                     LMNVLTIRQGDGVYVRDYMKGASIDFLRMFFLQTEEPDNEWVADPYILDEIWEFWTFF
                     LPEMLRLAIPKITPRDIKTLIDIFDEELANLHDKKHLIDLELLSQEAIAEAGNNIVVI
                     LITHTCRPLRKKMIEMFYDSLDEERIKKHIEIKKELVLSYLASATPDSLALIERYRET
                     LNGYRATLRKLLFEQEKASRRKK"
     gene            18258..19796
                     /locus_tag="PLG98_06995"
     CDS             18258..19796
                     /locus_tag="PLG98_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33039.1"
                     /translation="MHTQSTTVQNRFWGETTAFLALFLDNVGTLIFFSAILVFTFNYP
                     ADMILTRMIPGTAVGIFIGDIVYTWLALRLRKKTGRTDVTAMPLGIDTPSTIGIAYAV
                     MGPAYIATRDAQLTWHIGMATLFMIGAVKIVTSFFGGWIQRVIPTAGLLGPLAGIGLL
                     LLGFLPLVEIFNEAIVGMVALGLIFTTLMSKMQLPGRMPGVLAAVILGTGIHFFLGFS
                     GYLPEFAAPTLDMHFALPVPSVEFLKTLPQSVHYLTIAIPFGILTIIGGINNTESARL
                     AGDDYRTRDILLTEALSSLIASFFGGVAQTTPYIGHPAYKKMGATWWYTLLTGILIGV
                     CSIIGLLSVFVSLIPRAVIAPIFIFIGFEIMRQAYKDSPAAHSPAVSLSILPVIASMV
                     LIVLGQFTGASGIILEKLPGRLLIMHQTLTMLSNGFIITGLLWGSMLAFLIDHQAKRA
                     ALCAAVSAILTLFGIIHSVLPTGELYLPWHIQSQAHYMTALAYFTLSAILLLFNPPKA
                     EAQT"
     gene            19940..21637
                     /locus_tag="PLG98_07000"
     CDS             19940..21637
                     /locus_tag="PLG98_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOE33040.1"
                     /translation="MKFLPAVVSSFVESKIVKANIRLLVKYLIAIIALITTYSILFHY
                     IMAMEGQEHSWITGFYWTMVVMSTLGFGDITFTSDIGRLFSIIVLLSGVILLLILLPF
                     VFIKFFFAPWMEAQTRNRAPRELPVDTRNHVIITNFDPVTEALIEKLKSYNKNYVVIV
                     EDLAKALELYDRDIRVALGNIDDPETYNKMRVQNAELIVASNSDQMNTNITLTLREDL
                     EHIPIITTAESPHSVDILQLAGSSRVLQLYDIMGESLASWTVAGECKANIIGRHKELI
                     IAQTPVIGTPLVGQTLAESKLRETFGLTVVGIWERGKFIIPKSDYKINRTSVLVLAGL
                     ESSLVQYDDVYSFYHICKIAGDPVIIVGGGRVGRAVANHFQERDIPYLIIEKSRRKNE
                     DDSHFVYGDAADIHTLEKAWFEKAPAALITSHDDATNIYLTKYLRSLRPDMQILSRTS
                     EDRNISTLHRAGADFVMSYASLGANTIFNFLMNEETLLLAEGLNFFHLNTPRSLWGKT
                     LADSGIRERTGCTVVALSRKGETHINPEPDIRLGKEDELILIGTYETERAFLNEFPV"
BASE COUNT         5226 a         5635 c         5114 g         5805 t
ORIGIN      
        1 accgccatgg acgggaaacc cataatcgcc gcttcggttg ccgcggaaac cgttcccgaa
       61 tacagcacgt tgatcccggc attgccgccg cgattgattc ctgaaacgac gagatcgacg
      121 gggtgatccg ataactcttt aatgcccagt ttcacacagt ctgccggcgt tccgcaaacg
      181 gcatacccca aaaattgatt gtttttggtc gcccggcgca ccatcagggg acgggaaatc
      241 gtaatggcgt gtccaaccgc gctttgctcc gtttccggtg cgacaatcag acaatcggct
      301 tctttggaaa gttccttata aagagcgctc aatcccttgg cgtagattcc atcatcattg
      361 gtcaacaaaa aacgcattat ttttcatctt ctccgatctt gtttttttcg gccggcaacg
      421 gaaacagact gcctgcacgt tcatgttcct tctttaataa ttcccaggat ataattacgg
      481 gcgtttagcg ccagatcgcg tccttccagc aggacggaaa tttcattatt atatttcagc
      541 gcggattgcg tgagattatg gctgcccagg aaaatcagcc gctgatcaat caccgtcagt
      601 ttcgtatgcg tggttttgcg cggcgaatca aaataaaccc ggatcccctt tcctgccaga
      661 aattttcccg cctttctgtt ttgaatattc agcgcatccg atgaatctcc cgtattttcc
      721 agaaccacaa aaaccttcac cccccgtttt accgctttgg ccagatggcc ggcgatacgg
      781 tcaggataac tgttcttgtg tacgcccgcc ttaaaagaaa aaatggacat gataatctca
      841 tcctgcgctc catcgatggc gttcaacagt gcgggcagaa attcctcatt ggccagaatg
      901 accgttttgc attgatttgc ctgccgttct ttgcccgcgg caaaaacacc ggggataaat
      961 cccgaaatca aaaaaaccaa taccggcagg tagaaatatt tttttctgaa cgacaataca
     1021 ttcatgatcc tctccccttt atggtattgt atattatttc agcattccgc gcgatataaa
     1081 aaagcccgaa acggcagcag ccattccggg ctttggaaac actatggacc gcactatttc
     1141 acttcttcga aatccgcgtc aaccacgtcg tcgtttttct tgcccgatgc ctgctgatcg
     1201 ccgctctgct gttgagcccc gccatccgct cccggacctc cctgaggacc tgcggacttc
     1261 gcgtacatcg cttccgcgag tttgtgggat acgttcatca ggtcgtcctg agctttcttg
     1321 atggcttcaa catcgtcgcc ttccagggct ttctttgtcc tggcaatggc atcttcaatc
     1381 ttggtctttt ccgccgaatc gatcttatcg ccgaattcct tgatattctt ctcgacagaa
     1441 taaaccatcg agtcggcctg atttctggtt tcaatcagtt ctttgcggcg tttatcttct
     1501 tccgcgtgca tttccgcgtc cttgaccagt ttttcaatat ccgcgtcgct caaaccgctg
     1561 gacgccgtga tcttaatact ctgctccttg ccggtcccca gatccttggc attgacatgc
     1621 actataccgt tggcgtcaat gtcaaaagcc acttcgatct gcggtacccc gcgcggcgcg
     1681 ggcggaatac ccaccagttc aaagcgcccc agcgtccggt tatccgccgc catctggcgt
     1741 tcgccctgaa gaacatgaat gctcacagcc ggctgattat ccgccgctgt agagaaaatc
     1801 tggcttttct ttgtggggat agtcgtattt ttttcaatta atctggtcat cacgccgcca
     1861 agcgtttcaa tgcccagaga aagcggagtc acatccagaa gcagaacatc cttaacatcg
     1921 cccgacaaga cgccgccctg aatagccgcg ccgatggcaa caacttcatc ggggttaacg
     1981 cctctgtgag gctccttgtt gaaaatttcc ttcactttct gctgaacgcg gggcatgcgg
     2041 gtcatcccgc cgaccagaat gacttcgtca atgtctttgg gagacatgcc ggcatctttc
     2101 agcgccgtct ggcatggacc gaccacttga ttaatcagct cttcaaccag ggattccagc
     2161 ttggcgcggg tcagttttat attcaaatgt ttcggaccgg aagcatccgc ggtgataaac
     2221 ggcaggttga tatcggtttc catggtcgtg gaaagctcca tcttggcttt ttccgccgct
     2281 tctttcaggc gttgcagggc catcttgtcg tttcttaagt caatgccctg gtctttcttg
     2341 aattcggcaa ccagaaaatc catcacgcgc agatcaaaat cctcaccgcc caaatgggta
     2401 tcgccattgg tcgatttgac ttcaaacacg ccgtcgccca gttccagaat cgagatatcg
     2461 aatgttccgc cgcccagatc gaatacggcg atcttttcgt ccttcttttt atccagaccg
     2521 taggccagcg cggcagcggt cggctcattg atgatacgca taacattgag tccggcaatc
     2581 ttgccggcat ctttggtcgc ctgacgctga gaatcgttga agtacgccgg aacggtaata
     2641 actgcgtcct tgattttttc gcccagataa tcctcggcgg tctgtttcat tttagtcaga
     2701 atcatcgccg agatttccgc cggtgaataa ttcttgccgc gcacggtgac ctgcgcatcg
     2761 ccgttggaag cttccgttat tttgtaggga attatcttta aatcatactg aacctctttc
     2821 gatgtatatt tccggccgat caatcgcttg acggcataga cggtattttc cgagttggtg
     2881 atcgcctgcc ttttggcgac ttgtccgacc agacgctctc cgctctcaga aatggccacg
     2941 atggacggtg tcgtacggtt cccttcctga ttggcaataa ccacggggtc gccgccttcc
     3001 ataatagcca cgcatgagtt tgtggttcct aagtcaattc caataatttt tcccattgtt
     3061 tcctccttat tctcctgtat cctccatgat ttcaatcata tcatcttctt gttttttagt
     3121 tttcttgcac acacatactc tggcgggtct gaccagccgg ccgtttaaca aataaccttt
     3181 ttccatttca ctgacgactt tgttgtcttc atgctggtcg ctttccatct gcatcaacgc
     3241 ttcatgaaaa ttcggatcaa aatcctgacc ggcggcatca attctttcca cgccatattt
     3301 tttcaaacag cataaaagct gttcctgtat cagggcgatg ccttccctga aggtctgaac
     3361 atctccggct tcctgttcca gggccctgtc cagactatcc ataaaaggca ggatgtcttt
     3421 aataatttct tcgttagcat atttaatggt atcggcttta tccttgagcg cccttttttt
     3481 ataattatca aattccgcaa ccgcacgcag gtatctgtca taattggcag tcgcttcatt
     3541 ttccttttct tcaagctttt gctttaatgc ttcaacctct ttatttctgt cttcagcggc
     3601 atctttatct ttatgctcac cggcttgaaa acctttagcg ctgtgttccg actgttcttt
     3661 tttacttttc actgcaccct catgaatcat gcatgtaata ttattgaaaa tcaggttttt
     3721 gaaataattt tatatccacc agttcacaga tcgcgtgacc cgctgtaata tgcgtttcct
     3781 gaattcgggc ggtgctgttc gaagaaacat tgatggagaa atcgactatt ttagcgatat
     3841 ctcctccatc tttgccggta aaagccaccg tgacaagacc catttttttg gccatttcca
     3901 gacccttcaa aacattggca gaattgccgc tggtgctgat gccccaagca atatcaccgg
     3961 gctggccgat cgctctcacc tgtttgctga atatttcgga gaaatcataa tcatttccga
     4021 tactcgtaat caccgatgtg tccgtggtca gagcgattgc cggaagcgga ggtctttcga
     4081 taataaagcg gttgacgaat tcggccgcaa gatgttgggc atctgccgct gaaccgccat
     4141 taccgaacag caaaatttta ttgcccgctt tcaatgccga cgttatgacc tcgataacat
     4201 tcgccagttt tcccaaattt tcatttagaa agttatcttt gacgcgatta ctttccttaa
     4261 aaatttttat tatgtaatct tccatattta tatcattaag ccaagtgtca aattccgttt
     4321 cctgaagcgg acttaatata tttacggggt ttgggcatgt caagggaatg acaaaaattt
     4381 cttattttta ttcagtagat attttgccac gagcgcagat attatcgaca atcggagatg
     4441 gttgcgaatc ttgacaaata atttttcgta aatatattta aagtgaaaac agtgaaataa
     4501 ttaataatag cgaatagtta taaaaaaata atgttttcat gaaaggagga tggtctatgt
     4561 tcggtccaaa tctttggaga atgaaaagat tcgccgacag cgttccggaa ttaattgatt
     4621 tgcaaagaga aataaaccgg cttttttcaa gtgtcggcca ggctgctggc gcagattatc
     4681 ccgcaatcaa tgtctgggag aaagaggaca gtctggtggt gacaacagaa ctgccgggaa
     4741 tggatccgga cgatatcaac atttccgtta ccggttccat gctgaccatt tccggaaaga
     4801 gtaagggcga tccccttaaa gcaggggaga cttatctgcg tcaggaaaga gaactgggca
     4861 atttccagcg caatgttcaa ttacccttcc aggtggactc tccggctgtt gaagcaaaat
     4921 atgaaaaagg ggttctgaac attaccctgc ccagacaaaa agaagatttg cccagaaaaa
     4981 ttcaaattac tgcataaaag gaggtcgttg aattatgtcc gataaagatc tgcaaaagac
     5041 agaaaacacg gcagccacag aaagaatacg caatgttaaa acttttgtgc cgcgtgtgga
     5101 tatttatgaa acgaaagacg ctctttttct gattgccgat atgcccggaa tcgatgaaca
     5161 aacggttgat gtggagctgg aaaaaaacat cctcaccatt tccggacgtg tggaaaacgg
     5221 caaggtcaaa gactatcgcc ttgtcttttc cgaatatgaa gtcggcgatt acgaaaggac
     5281 attcacactg tcagacgaaa tcgacagaga caaaatcaag gcaaacgtga aacacggtgt
     5341 attaaggctg gaactgccca aagcggaaga ggtaaaaccg aaaaaaatta aaattaacgc
     5401 ggcgtaacaa aacgtgaaga gttgcgtcat tacattttta aagaaaggag gaagtgacgg
     5461 atatgatgat taagaattta ttacccgatg tcgtccgaag aacaagggca gaccttgatc
     5521 aaccttttta ttccctgcag aaagaaatga acaatctgtt cgatagtttc ttccggggga
     5581 ttgagccggc tccgcgtgga ttcgccggat acttttctga agctttttct ccttcgattg
     5641 atgtaaaaga gaacgacaaa gagttcatca tcaaggcgga attgcccggc gtggaagaga
     5701 aggatattga tgtgacggtt accggcgatt ccgtgaccat caggggagaa aagaaggaag
     5761 aaaaggaaga caaggataaa aactactatt acatggaaag gtcatacggt tcattctgca
     5821 gggtgattcc cctgcaaaca gaaattgaat cgggcaaagc ggaggcgcgt tttaaaaacg
     5881 gaattctgga catcaagatt cccaagagcc agagtgccaa agcgaaaggg accaaggttt
     5941 ccatcaagag cggctaatct gcaaagcccg cctcttttcc aaggggcggg cttttccaga
     6001 atcttaaacg ttttcttggg gcctaattgt acccttcgtc gtaagcaatc atatccagat
     6061 gcaacgtggc aatatcttca acatccatat tgggttcgtc gctgaatttg ggcaattcca
     6121 ctgttttgat gtagcgccag cacttgttgc agacatccac ccggtagcgc tcttcacctt
     6181 ccacttcaaa ataggccagt gattgctgtt cgtcgttgcc gcagaaaggg catttgatgc
     6241 ggtagaaata ccatttgaac ccgcactgat ggcagaacag ataccgtttg ccctcttcat
     6301 cttttctgat ttcaccgatc ttcggttcct tgccgcaaat cggacagtag ccttccgtcc
     6361 agtcggattt cgcgaccatc tctccgtatt ttttggcgat gatttccagc tccggcctga
     6421 gactttcttc gagaaaaaga tcgatcagat caatattgtt ttcttcttcc cggtccgcct
     6481 ccgccgtccg ggcatcggct tcttcaacct tacagtcaaa ggacgcgcga acacttttct
     6541 cccagtcgaa atcgggatca tcaacgatat cggaaatgct ttgctccagt tcaggtatac
     6601 gtttttccgc cgcctgaatc agggcgtaaa aatagtcccg ggggcgtttt aaatcaaatt
     6661 ccttatccgt cagatcaatc aagggcagtc cgcctgtcat tttctgagca attaagtttt
     6721 catcaacatg aaaaatggga cgggacatgt ttttgagata ctcttccctt aaaatcagaa
     6781 tgtcagccat gatgtccagg agttccgtat aatgaggatt ggctgattta taatcctcaa
     6841 tcgtttttaa agatttgcgc agtgtaccga ccatgctttc atactcctgt gagacgttta
     6901 tttagatttg ttatcctgaa tgtcactgaa taatccgatt ttcattagaa caaaactgat
     6961 cgacctatat ccactttccc gtgtatcttc aagtagtttt ttcacaggta acaaaacagc
     7021 ggcggcggca tcaactcccg gataaaatca gccataagta tccggatatc ctatggatat
     7081 ctttttaaaa gacttctgca aatcatgaca aaatgatgcc ggcggcatcc gatggcaaat
     7141 ataagccgaa cgatgcgcaa aaacgcgctc tgtcggcaaa aaactccagc catccggccg
     7201 ggtctatttc atctttgggt ttaacgtgcc atttttgcct gcggctttaa tccacttgac
     7261 acacaggata ccgtttcata tagtccagac tcgctaaaag gaagttttac cataaatgtt
     7321 tcggtatgaa ttcgtaacac aaattgagat tgacgcgaca gcggagcgag tatggaacgt
     7381 cctcaccgac ttggattcct ttccccagtg gaatccgatg attcgctggg caaaaggaaa
     7441 agtatgctcg ggagagaggc tgaccgtccg gtttgaaccg aagggaacca ggggatatac
     7501 ttttacgcca aagctgctga tcgttgaacc gaaccggcaa ctgcgctggc tgggataccc
     7561 ccggatacct gcattcatgg ataccgaaca ttactggctc atcgaacaga taaccggtga
     7621 aaaatcccgt ctgttgcatg gggctatcat ttacggcttg ctcacaccgg tgagccggat
     7681 tatttttgag aacacctccc ggggtccctt tgagcagatg aaccacgctc acaaaaaacg
     7741 cgccgaatca cgtgaatgaa tagtgatccg gattaatatt ttcttggcaa cctcatatca
     7801 gacaactgag tcagcctctt ataaaaatat ttcggatcaa aatttcgttt gatggaaatg
     7861 gcgccgtggg gacaggcggc gccgcagtcg aaacaggcga tacaaagcgt ggctcccgtc
     7921 tttaagacat ccacacgagg caccccgctt tttacggcct ttttcatgtc ttgcatgaga
     7981 tctttcttgg aaattttgtc ggtaacaatg catccacccg gacatatctt gacacatgtg
     8041 ccgcactgct tgcatttttt agaattaaag aataaatccg ccgttttcac atgattcgga
     8101 ttgtcatagg tcggtatccg ccatcgatcg atcacgctga attttttcat atcattcccc
     8161 ctgcttggta attcttggag cgtcgttcat gcctctttcg taccattgaa ccaactgcac
     8221 ctcgcgcggc accatgccat cgaccctcgg gttgggccaa ccgagagcga tacattcgtt
     8281 gagcttataa ggatatttga tgccgaattt ttttctccat ttaggcagat acatcaatac
     8341 ctgaatgaga ccgatccagc agcttcccgc ccccaggctg tgcgcggtca ggaccatgtt
     8401 ctgcccgcaa attccgatat cggtgggcgg gctggccaca ccccgtctgt cctccgccag
     8461 aaggatcaac gtcggcgcgc cgtgaaagac aggcgcccgg ccctgggcaa tctgcgacat
     8521 cagcccgaac ggaacaggat gaagtttgtt tttcaaaatc cggatgatcc atttgaccca
     8581 gctcagaata tagagtcggc gcagtatatt gccccgggtg taatcaatga aaaacatgaa
     8641 gaactttatg attttcagcg cgtctttttc catttctgca agaatttcag ggctgttgac
     8701 gacaatgaat ttccagggtt gtgaatttcc ggctgacggg gcaaaccggc cggcttccag
     8761 aattctttcg atcatgcggt ccggcaacgg ttctttttta aaagcccgga tgctgcgccg
     8821 ctccatgatg actttttcaa cgggattcca ggccatgcct tcttcgtatt ttcccaacgg
     8881 agacaatacg tcatctttcg aactcatatt tcctccttac ggttgattta ctgcaaacct
     8941 tttgagacgt ccttgaaacg ttatgcaatc ttcgtgttac ccacgtactg atttttctgg
     9001 gcgtcatacg ttcctgccac aacgccgatc ttctccagtt cttttttgat gattcgctgt
     9061 gtttcggttt tgggaaattc cctgacgatg cggacaaaac gcggaacggc aaatttggcc
     9121 aaatcgtctt tgagaaaggc tatcagatcc gcttctttca gcgtttgccc ttccacgaga
     9181 gaaacgctcg ccataatttc atcctccgcc agttccgacg gaacggcgta gacggccgct
     9241 tccagcacgg aaggatgttt ctgaatggta tgttcgactt cgtacgcgga aacattttcg
     9301 cccttgatgc gcatgaactc cgcgttgcgt ccgacaaaat agaggtagcc gtttttgtcg
     9361 cgcttcacga gatcgccggt gtagatccag ccgttgcgca atttgtcatt gctggctttc
     9421 tcgtttttaa aatactccac cgattttttc ttgcctttgg attcgaaaat gagctgtccc
     9481 ggcgtgccgt ccggaacatc caggccttcg ttgtccacca gccggtatac cgtgttgggc
     9541 gcgggtttcc cgatagagcc gaccggggcg ttgcccaagt tcatgatgct tttgccgccg
     9601 ccgtccacgg aaccgtacgc ttcataaatt ttgatgccga atcgtctttc gaacttcccc
     9661 cactcatcca ccgggcaggc agccgacaac gtaaagcgca cgctgttgac gctgtcgttg
     9721 tcgcggggcg gctgcttcat cagaatcggg atcatggcgc cgatggtatt gaaggcggta
     9781 atcttgtatt tgcgcatgtc atcccagaac cggctggccg aaaattttct gcccagaacg
     9841 acccggcaac cgcggtgcat gccctgtgtc acgcacagcc agagcgcgtt gccgtgaaac
     9901 aggggaaggc aggtgtaaag aatgtccgat cttttgtaca gcgcgtacgc gggaatggaa
     9961 agcagcttca aagtggtttt gccgtagcgg tacatgacgc ccttgggcag gccggttgtt
    10021 cccgacgtat aaaggatgaa gcacacgtcg tttttatcat agggcacatt cggtttccgg
    10081 ggcgattgtt tataagcatc ggccaggttc aacaggccgt gaggcagacc ggaggcaccc
    10141 ggcggtacgg agcggttgac aattaccgtc ttcttccgtt cgagcctgtc cgctatttta
    10201 ttgtacgcgt cgagaaattc ttcgtcgatc acgatgaatt ccgcgtcgct gtggttcagg
    10261 atatagagca gactgtcgcc gcgtaaagag gtgttgatgg gaatggaata catgccgagt
    10321 ttttgcgacc cgatgtacac atcaagatat tcgggacagt tggccatgat gatggcgaca
    10381 ccctttgctt ttccggcgcc gagcgactgc agaaaattgg ccaccctgtt ggcgttttcg
    10441 tccatctgcc ggaaggaaaa ctcccggtct tcgtaaatca gaaatgtccg gtcgccgtat
    10501 tggcgggctt tttcttcgct gagttcggcc caggacatgt cttccgtaat ttcctgcgtt
    10561 ttcatcaggc tgctgaaatt cgcccggaac gtcccgcgcc tgatttcatc cttgaaaaat
    10621 gtcaataaac ttttgatcat ggacgaaccg attttcaaat ttgccagcaa atacatcttc
    10681 actcctcatc cttctttttt agtcatccgg aacccgtgac agcgcgcgtg gagattccac
    10741 tatgacaaca tgtccctgat cagagacagc ttccgcatgg gagccatccc cagcagcgcc
    10801 tggacatgct tggcgtcgcg gaatcttttt tcctgtccgt aatctttcat atagccgttg
    10861 ccgcccagac actggatgcc gtcggcggtc agtttgcagg ccagttccga caggtgcagc
    10921 gccgccgctc tggtgcaaag cccccactgc ttctcctggt tctccaccgc catggccgcc
    10981 tcggaaacca ccatctcggc gattttcacc tgcacggcca tctgagccag cagcatgcgc
    11041 aatcccgacc agttgatgat ttcgcggccg ccctgacaac gctgccggct gtaatcgaac
    11101 gcttcctgaa acgatcccct cataataccg cactgaatgg ccgccgccgc cgcatgcatc
    11161 cggtcggagg cctgattgaa atatttgatt ccttctcccg cctgaccgat cagttgtcct
    11221 tccacagcgg aaagcgttaa atccacggcg ggacaggcat gcagtcccag gctgaagacg
    11281 ggctcgcttt tggtaacgcc tttatccgcc ggacgaatca ggaaaaagct gaagccttcc
    11341 tctcctttaa ttttaaccgg aacaagcgcc tggccggcca ggccgcccag gacaatgtat
    11401 tccatggcgc cgctcagcag atatgtgtct ttggatttaa cggcttcgag ggaaacgccg
    11461 ttttccgcag gattggaaaa agatggaaag gcgatgagcg ccgctctggc gtcggccgct
    11521 ttttccatca gagcggtcag cagattttta tcgcccgccg cgaggacgat ttcctgagcc
    11581 agagcgttcg taaaaataat tccggccagg ctggcatccg ctgcgctgat gtggtcgaga
    11641 atgatgcaca aggccccgat atcctggccg atgccgccgc attcttcggg cagcgtcgca
    11701 ccgagcagtc cgacgtcatg cgctttgtcg atcacggaat caaagagggg accgaaagga
    11761 taacggtcgt ttgcttcccg gttggcggca agttccttcg ccgcaaaatt gtgtgccaga
    11821 tcttcaaaag acttgagttc gcattttacg gttgtcagca ttctcatccc tcctattctt
    11881 cgaatacacg ggcctgcggc acgcaacgcg cgatcaggca attaaaaaga ttgaggcggt
    11941 tcaactgatt ggtgccttca taaatttgca gcaacttcga atcgcgcagg aatttttccg
    12001 cgccgcggtc gtgacgcaat ccggcctgcc ccatcaattc gagcgccatc tggctgttct
    12061 tcaaccccat atcggtgccg aagaatttgg caatcgacga ccacccggaa cagcgtttct
    12121 gcgcctccat cgattgtcct tccacgaaaa tctgacgaat cgcccagtcg gtcattttaa
    12181 accggtagaa gggaacgatg aatgtatcga tcacaatctg cggaacatac ttgagatagt
    12241 agtaaagcgg tttcacctgc aggaaattaa aaatgccgcc cttcaggctg ttggaataat
    12301 tggcctcgtt ataagccagc cgtccggcaa tcacattttt atacatctcg gccagcataa
    12361 tctgcgccca ctcgtgattg ataaggagtt tgccgtccac ctcggtctcg ccggcaaatt
    12421 tcagcgcatg ttcataagct ccgcgcgcca cgccggttcc gaaggcgcct accgacgcac
    12481 gggtcacctc gacaacatag tggatgatct gattgacgag atcggaggtc tcgcgaatct
    12541 gggagttggg gaaatcgcgt gaatcaccca aaacatattc atccggtatg aaacaatcct
    12601 gaaaaatcaa ttcgctcgcg cagcaggcgc gctgccccat cttgtcttca tgcttgccga
    12661 aggtgaatcc cttcatcccg gtcctgacgg caagcattaa cgtattggcg gaaggttttt
    12721 tcagatcggt ataggcgatc agggcgtgcc aggtggatac atgtcccatg gaaataaaaa
    12781 tcttgctgcc gttgacgatg tatccgccct tgaccttttc ggcaaaacag gtcaccttgc
    12841 cgcgatcgac cagttccact tcctccacgt ccgtaccggc gccgggttca gtaatcgcca
    12901 gcgagatcag acagggcttg cccgtttttt ctccttcggc gacttcacga cagactcgat
    12961 tgatgagcgg caggttccag gaagccagca gggtcataaa accgaggtaa tgaacaaacg
    13021 cgacgttggc aatgccgacg caggccgaag acaactcctc aatgacgtaa gcgtggctgg
    13081 ggagactcca gccctggccg ccgaaaattt tggggatgaa catggtatag aatccccatt
    13141 cgttggcctt ttcaataatt tcccagggaa gatagtcatg atcctgaaag gtttttaaat
    13201 cggttttcag cgccagcggc ctgacgactt catcgttgaa gcgacgggcg tgactgacga
    13261 gcgtcctggt ttccttgagc tggattttcg gaattttcgg aatggcctgc acactgcaga
    13321 aactgtcctc gaaacgcggt tcacggtcta ataaagattt gacctccttc ggttttatca
    13381 taatccccct ccctttgtgg attgactgca acaatcttac ccttttgaag actgctcccc
    13441 cttctgcaaa ccagggagcc ggataccgtg ataacgctga atctttttct ttcgagctgt
    13501 aaaacgtcac gggtgacgta aatcttttca ggatgagggt gcttcaagaa tatgaataca
    13561 catggccgct tcctcggtcc ccaaggcgcc tccgccgttt tccgccaggg ccagtctggc
    13621 gttattcacc tgacgttttc cggcattgcc ccgcaactgg gtaaccagtt catggacctg
    13681 agccagaccg gaagcgccga tgggatgacc gcgtgaaatc aggcctccgc tgggattgat
    13741 gggaatgcgg cccttcaggc ttgtttcgcc tttttcggca aaaattccgc cttcgccttt
    13801 tggacagaag cccagatttt ccgcctgcac cacttcaccg aaggccgaag catcatgcac
    13861 ttcagcaacg ttgatgtcct gcggaccgac gccggctttt tcataagcct gtttggagag
    13921 tctctcgccg atttccggaa tgtcggaatc aaaagatgtt ctgatggccg aaccgagaat
    13981 ggaagccctg atcttaacag cttgatgggt caggccgagt tttttcacca tgtcttcaga
    14041 acataaaata gctgacgcag cgccatcgcc aatgggcgcg cacatggagc gcgtgagcgg
    14101 ccaggagacg atgcggtcgg ataaaacttt ttccacgggt acgtcaaagc gatattgagc
    14161 gttgggattg agtgtggaat tgaaatgatt cttggaggca atcatggcca tctgttccgc
    14221 cgttgaacca tattttttca tgtgctggcg ggcggcgaaa ccgtaaacat ccatgaacgg
    14281 cgagtgatct cctgatgaca tcctggccag ctgccgcacg gtatcgtaac cgattttttc
    14341 acccagaaca ataaaggcgg taaacttgtc tcgataggcc ttccattttg attcttttgc
    14401 tttggatttt ttcacgccgc cattgtttgc cgcctttttc accgcctggt agcgctgctt
    14461 aaattcttcc acttttacct tctcttccgg cgtgagagta aaaacagaaa gggttttagc
    14521 gatattcaga acgttctcga tatcgatgcc tcccaaaaat ccgaagaaga cgagattctt
    14581 attggaaaaa taaagttttt ccgcgccgac ggccatggtg atatcataca aaccgccggc
    14641 aactccgtac caggcatgat gcagcgccgt ggatccaccg gcgcacgcgt tttccacatt
    14701 ggtgatggga atgccttcga ttcccatgcc cttgagggcg acctggccgc gaatagtcat
    14761 ctgccctcct tcaccccagc cggcattggc aaaccagaga gcctgaatat cttttttatc
    14821 cagaccggca tctttgagac atccggtggc agtcgtaatc gccagatcct tgatgctctt
    14881 atccatgtgt ttgccgaatt tgattgtatc aaccccgatg atatagacgt ctcgcatatt
    14941 atttctccat tttttgaata tctttcttta caacaaatat attattgccc tcatatattt
    15001 accgcatgaa caattcttta aaacgaacga tccccgcaga ttatcggctc ccgattgttc
    15061 gttcacttcg gttttataaa tttacccagc agtatcataa ttttacttga gaaccagtcc
    15121 atcagccaga ttggcgtaac attgtggcaa tagactaaga acctggcctc ccaacccacg
    15181 aggtagcgag cctttggcct ttttacgctg agggcgcgaa tgatcacccg gacggccttt
    15241 ttcatggata tccccatttc cgcctgccat tttggaaaga cgctgagggt ccttagctcg
    15301 tctttataga ggtctcgggc ctgtttcggt acggatgcaa ataatttttc ggtggtggca
    15361 tctatttttt cccacatggg tgttttaatg gtctgcaatt ccacggacga gactctgatt
    15421 ttccacgggc gaagttctat ccggagagcg ctggcgacgg cctcaagagc aaattttgat
    15481 gaggagtatg gtgccatgag cggtaatgtg accctgccgc tctcggaact cgtgaacacg
    15541 attctcccct gcgccttgcg caggagcggc agaaaggtct gcgtcacgaa gatgtgaccg
    15601 aaaacgttga cccgtatctg ttgttcaaat aattcaatgg gcagtatttc caatggcccc
    15661 tggaccgaaa tccccgcgtt gttgaacagt gcgtccaggc ccgcgccgcc gagggccttt
    15721 tcgatctcgc tcgcgcctcg tttcacgctt tcatagtcga cgatgtccat gatcaccgtc
    15781 gtgattttgc cggaggaggc gtcacgcagt tttttggcgt cggcctcttt tctaacggcg
    15841 gcaaatacac gccacccctt ttgagccagt tccagcgatg ttgcccaacc gattccgctg
    15901 gacgcgccag tcacgagtat tgcattcgct gatgaattga tttttgaatt tgcgcccatg
    15961 gttgttttct tcatggcccg tatcctcatc aatgtttaaa tttataaagt ttgccttctt
    16021 ccatatgctt cacggtatcc tgtacaactt gattaatttc ttcagtcgtc atcttctgag
    16081 gatccatggc attaatatgt tgatccaagt aacgaatgta tgcctgcatg gcattcatgg
    16141 tgtcatccgt atccagacag cgaatgctct gttccatttc gatgctcaaa ccgtgtctta
    16201 tagttgtttc cattccgtta tggatgcact tcttgatcgc atccacagcc gtcatgggac
    16261 gcttgctcat caaatcggca aattcctgta ctttggtctt gaattccgct ttattaaaaa
    16321 aaccggtaag caaacccacg cggtgtgcct cctcagcgtc ccactgatta cctcgcaaca
    16381 tcagttccag cgctttggct ttgccgatca gccggggaag cctttgcgta gaaccgccgc
    16441 cgggaacaat gttaatcagg acttccggtt gtccgatcgt aaatccctga tcgcccaccg
    16501 caaatcggaa atcgaaacac gcggataatt cggtgccgcc gccgttgcag ttgccgttaa
    16561 tcgcggcaat ggtaatttta ttcagccgtt caatcgccat gtaaagcctt tgcatgatga
    16621 accacaaata caggctggat gaatagccgc tcaaaacttt ggcaaatttc agcaccagct
    16681 tctcatacca tccgaagcca tccatcaacc cgttcgtgca ggttgtaaag tgcttcagga
    16741 tagcagctgt cagtctggtt tttatgaaaa ggttgagccg tatttttttg ttgtcctttg
    16801 ataacgtgaa aagttcggga atagaaaaat gcatgatgta aatatcttcg ataccgcccg
    16861 tcaggataaa tacccggatg gaaggatccc gggagacttg tttgacgagg tcaaagagct
    16921 cttcgagcat cgccacggtc aggaaattaa ccggcggatt tttcatttcc acccaaaggc
    16981 tatggttttc tttttgtatt tttaaatatt tataatccat tttcatagtt tgcatttcgt
    17041 aattagaaga cggacctata tttaatctac aaaaaaagac gtcttgtttt gaagacctct
    17101 tttcaattga tcaacttcgt acttgatcat gccgagcaaa ggtggtatat taaaaacgta
    17161 taattggttt gaccaatgaa taacatatat ttaccacatg tcaagtattt tttacttttt
    17221 ttgaagggca tgtattcctt atggagtcat tatggaagaa cttcagtcac atgagagggt
    17281 tatgaatatt ctggtccggc agattttcag caggcagttg aagcccggcg ataagcttcc
    17341 ttccgaacgg caactgtcca aagatatgga tgtggacaga gcgtcgctga gaatcgccct
    17401 caagcatctg gaactgatga acgtgctgac catccggcag ggcgacggcg tttatgtccg
    17461 cgactatatg aagggcgcca gcatcgattt tctaagaatg ttttttctgc aaacggaaga
    17521 gccggataat gaatgggtgg cggatcctta tattctggat gagatctggg aattctggac
    17581 ctttttcctg ccggagatgc ttagactcgc catccccaaa attacgcccc gcgatattaa
    17641 aaccttgatt gatatttttg atgaagagct ggccaatttg cacgataaaa aacatttgat
    17701 tgatctggag ctcctgtcac aggaagccat cgcggaagca ggtaacaaca ttgtcgttat
    17761 cctcatcaca cacacctgcc gtcccctgcg caaaaaaatg atcgaaatgt tttatgattc
    17821 tcttgatgaa gaacgtatca aaaagcacat cgagatcaag aaagaactgg tgctttccta
    17881 tctggcgagt gcaaccccgg acagtctggc gctcattgaa cgatatcgcg aaacactcaa
    17941 tggttatcgc gccaccctgc gcaaattgct ttttgagcag gagaaagcct caagacgtaa
    18001 aaagtaattc cgaagcatgg cccgtctatt aaaaaaaatc cggacatccc tattaagggg
    18061 aaaatcattg cttgatccat gcttctcata aaattctttt aacggaaaac gcattgtcaa
    18121 acagcgtttt tttgtttata cagttatcgt ataagcaata tcaacattat tgcatatcaa
    18181 gatttctccc cggcatagat ggacgataac cttcagcata cgtgacactc aggaaaacgg
    18241 aagattgagg aaatagaatg catacacaaa gcacaaccgt tcagaacaga ttctggggag
    18301 agaccaccgc ttttcttgct ttatttctgg ataatgtcgg aacactgatt tttttcagcg
    18361 ccatcctggt ttttacgttc aactatccgg cggacatgat cctcacccgc atgattcccg
    18421 gtacggcggt cggcatcttt atcggagata tcgtctatac ctggctggcg ctgcgtctga
    18481 gaaaaaaaac ggggaggacc gatgtcacgg ccatgccgct gggaatcgat acgccctcca
    18541 ccatcggcat tgcctacgct gttatgggcc ccgcgtatat cgcgacacgc gacgcgcaat
    18601 tgacctggca tatcggcatg gccacccttt tcatgatcgg cgccgtgaag atcgtcacgt
    18661 cttttttcgg cggatggatc cagcgcgtta ttcccaccgc cggacttctg gggccgctgg
    18721 ccggtatcgg tcttctactc ctcggcttct tgccgcttgt tgaaattttc aatgaagcaa
    18781 tcgtcggcat ggtggcgctg gggctgatct tcacaactct catgagcaaa atgcaactgc
    18841 ccggacggat gcccggcgtg ctggcggcgg taattttagg caccggaatt cattttttcc
    18901 tgggattcag cggatacctg ccggagtttg ccgcgccgac tctggacatg cactttgctc
    18961 tgccggtgcc gtccgtcgaa tttctgaaaa ccctgccgca gagcgttcat tatctgacca
    19021 tcgccattcc cttcgggatt ctgaccatca tcggcggtat caacaacacc gaaagcgcgc
    19081 gtctggccgg agacgattac cgcacccgcg atattctgct gacggaggcg ctgtcctcgc
    19141 tgatcgcgtc cttcttcggc ggtgtggcgc agaccacacc ctacatcggt catccggctt
    19201 acaaaaaaat gggggccacc tggtggtata cgctgctgac aggcatcctg atcggcgtct
    19261 gctccatcat cgggctgctt tcggtttttg tcagcctgat tccgcgggcc gtgattgcgc
    19321 cgattttcat ctttatcggt ttcgagatca tgcggcaggc gtataaggat tcgccggccg
    19381 cccactcccc ggcggtcagc ttaagcattc tgccggttat cgcaagcatg gtgctgattg
    19441 ttctgggaca gttcacgggc gcgtcgggca tcatcctgga aaaactcccc ggacgattgc
    19501 tgatcatgca tcagacgctg acgatgctca gcaacggatt catcatcacg gggcttctct
    19561 ggggaagcat gctggccttc ttgatcgacc atcaggcgaa acgtgcggct ttatgcgccg
    19621 cggtcagcgc catcctgacc ctcttcggca tcattcactc ggttttgccg acgggcgaac
    19681 tgtatctgcc gtggcacatt cagtctcagg cgcattatat gacggccctg gcatatttca
    19741 ccctgtccgc gatcctgctt ctattcaatc ctccaaaggc tgaggcacag acataatgtc
    19801 gattggcgaa ggcgagggtc cggtgcggtt ttgcccgtga tgatttccat tgacaggcaa
    19861 aacggttctt tctatacttt gcaatacgca gacaaatcac gtcgctcaaa atcagttccg
    19921 tctcaaggat gccaaccgta tgaaattttt acccgcagtc gtcagttctt ttgtcgagag
    19981 taaaattgtc aaagccaaca ttcgtcttct ggtgaaatat ctgattgcga ttattgcgct
    20041 gattaccacc tacagcatcc tgttccatta catcatggcc atggaaggac aggaacactc
    20101 gtggatcacc ggattttact ggacgatggt ggtcatgtcc acactggggt tcggggacat
    20161 tacgttcacc agtgacatcg gaagactttt ttccatcatc gttctgcttt ccggtgttat
    20221 tctgctgctg attcttctgc ctttcgtctt catcaaattc ttttttgcgc cctggatgga
    20281 agcgcaaacc cgaaaccgcg cgcctcggga acttcccgtg gacacgagaa atcatgtcat
    20341 tatcaccaat ttcgatccgg taacggaggc tctgattgaa aaactgaaaa gctacaataa
    20401 aaactatgtg gtgatcgtgg aggatctggc caaggctctg gaattgtacg accgggatat
    20461 tcgcgttgcg ctcggcaata tcgatgatcc cgaaacatac aacaaaatgc gcgtacagaa
    20521 tgccgagttg attgtggcat caaacagcga ccagatgaac acgaatatca ccctaaccct
    20581 ccgggaggat ctggaacata ttccgattat cacaacggcg gagtcccctc attccgttga
    20641 tattctgcaa ctggcgggaa gctcacgggt cttgcagctt tacgatatca tgggagaatc
    20701 tctggccagt tggactgtcg ccggagaatg caaagccaat atcatcggcc ggcacaagga
    20761 attgattatc gcccagacac cggtcatcgg cacccctctg gtcggtcaga cgcttgccga
    20821 aagtaaactt cgcgaaacct tcggcttaac ggtggtggga atctgggaac gcggaaaatt
    20881 cattattccg aaatccgatt ataaaatcaa tcgaaccagc gtgctggtgc tggcgggcct
    20941 ggaatcctcg cttgtccaat atgatgacgt ctattcattt tatcacatct gcaaaattgc
    21001 cggcgatccg gtcatcatcg tcggcggagg aagggttgga cgcgccgtgg ccaatcactt
    21061 tcaagagcgg gacattccct atctgatcat tgaaaaaagc cgccggaaaa atgaggatga
    21121 ttcgcatttc gtatatgggg atgccgccga cattcatact ctggagaaag cctggtttga
    21181 aaaagcgccc gctgccctga tcacatcgca tgacgacgcc accaatattt atctcacaaa
    21241 atatctgcgc agcctgcggc cggatatgca gattctcagc cgcaccagcg aagaccgcaa
    21301 catatccaca ctgcatcgcg ccggcgccga tttcgtcatg tcgtatgcgt cgctgggcgc
    21361 caatactatt tttaatttcc tgatgaatga ggaaacactg ctgctggccg aagggttgaa
    21421 cttcttccat ctgaataccc cccgatcgct ctgggggaaa acactggccg actccggcat
    21481 cagagaaaga acgggctgca cggtcgttgc cctgtcgcgc aaaggggaaa cgcatatcaa
    21541 tcctgaaccg gacatcaggc ttggcaagga agatgaattg atcctgatcg gcacctatga
    21601 aacggagcga gccttcctca acgaattccc cgtttaaaaa tctgacggga ataagccata
    21661 ccgcctccgt tgaagcggac taaagcactt ataaaaaaag ggcctggaaa aattccagac
    21721 ccttttgaat tatatttcaa ttcgacttac tttttatcgt cggcgatcat gagtttgcct
//
LOCUS       DAOSME010000038        21774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3000_length_21774_cov_19.342926, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000038 DAOSME010000000
VERSION     DAOSME010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21774
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3000_length_21774_cov_19.342926"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLG98_07005"
     CDS             complement(<1..196)
                     /locus_tag="PLG98_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33041.1"
                     /translation="MPNNSTTTPYVRLGFLDSLRCFAIIYVVISHLILIPQPNLAIPE
                     WLAPFLINGGDAGVSLFFVLS"
     gene            complement(247..1578)
                     /gene="purD"
                     /locus_tag="PLG98_07010"
     CDS             complement(247..1578)
                     /gene="purD"
                     /locus_tag="PLG98_07010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOE33042.1"
                     /translation="MPNILLLGNGAREHALAEAITRSGQKPSLFSFMKSNNPGIASLS
                     EKTLIGSYADLDAITKFATENKIEFAVIGPEDPLNNGVADALAKINIPAVGPTKSLAR
                     LETSKSFTRNLVSKYNIPGNPQFKVFKTLDGVEDFLNQLEGIVLKPDGLTGGKGVQVQ
                     GDHFDSKKEALKLCAQILAGGASLVIEEKFDGEEFSLQCLCDGKTVVATPLVQDHKRR
                     FDGDKGPNTGGMGSYSLADHSMPFLRPQDLHDGLEITRQVAEALLKETGSPYKGVMYG
                     GFIATKDGVKLLEYNARFGDPEAMNILPLLKTDFVDVCRHIIAGTLDQIEIEFDKKAT
                     VCKYVVPKGYGLPADHPDAASSRAKIEVGNVGPARLYYSSVDQKDDGLYLSSSRAIGI
                     VGIADNLADARKIAEEGVKAVKGPVAYREDIGTDALIQKRIDHMKKIRKDA"
     assembly_gap    2032..2131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2132..3284
                     /locus_tag="PLG98_07015"
     CDS             <2132..3284
                     /locus_tag="PLG98_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE33043.1"
                     /translation="QNNYPFFNYVVKLLSNQRRLLITIVTSNEVVNVSISILAASFFI
                     GLFGSSGQWVSIVFTTVVLLFVGEAIPKTFGVTYPMQISSLVSPLLMAISRIESPIVS
                     ALEKVSGLFLRRYGVNKSEESEVIMEHEFKTLVDVGSLEGVVNEAQKELINKIFELGD
                     RPVTDIMVPRVDMFCLPLDMPVGEMLQSIAAARQERVPVYREDRDDIVGILFARDLLN
                     NVWRGRSQTGIYNLLVRPFFVPEQKTINGLLHDFQKNFIQIAIVVDEYGGVSGMITLE
                     HILEHLFEEPEGDEEMVCDGCEKIDEQSVIVPGSMSVEQCNKLLEAELPEDEFDTVGG
                     LVLHLFGKIPQKGEEAHLDGYIFCVHDVGKTRILKVRITKKTVEEPEES"
     gene            3281..4561
                     /locus_tag="PLG98_07020"
     CDS             3281..4561
                     /locus_tag="PLG98_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE33044.1"
                     /translation="MSLYLIVAAILFCLMLEMLYSGGEVALFASDINKLKNRAHQGST
                     SAHQAVKLKEKPEWFISTALVGTNLAIIIASTLITGLLISFYGPARGEKIAFLIMIPT
                     LFLMIIARSIFLYYAEAMAMRVAWFIRFSSILFYPIAFVIAAVSRSTVHFSSDTRSGQ
                     SSHITKEGLKYILGEKTKGGDILTREKEMVSRVFDFSELTAMKIMVPVSALTALSASM
                     KISEAVGVVADKKYMRIPVYEDTVYNIVGILRYFDLFEMLLKDKEKGEGVGEATVASC
                     MRKDVFYVPETKKVSELLAAMQKRDEQMAVVVDEYGGATGVVTIEDIGEEIVGTIDEY
                     VSGEKLYRKIAEGRYLVSGRLEIGTLRQLVKAKFPEGNYETVGGFLMYQMGKIPRIKE
                     YYQYGDVTFIIENADQKSIKEVTVIAPFIQEGNK"
     gene            4679..5878
                     /locus_tag="PLG98_07025"
     CDS             4679..5878
                     /locus_tag="PLG98_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33045.1"
                     /translation="MKSDLTRQDWEEYLIRLYFGTKNDLIKSCINRAYRDFNRTLHGM
                     DDHKNKQDLFDRAVKYLLSIFIDIKKESLNDHEEFDKWHKEKCLHLKNLYKENDFINF
                     SIGQAQKWINMTFKYIFCVGEKRLPGYSGLYEFCHVPIDNIILDKIKKRGAPVPSKPW
                     SRLGDYNEYISLQKWFREQYKLPLDGEFILWMEEPHDNVIMPVQNNIVLDKTRVKEAK
                     DETIGPYQLNRIILCSADASPASQKKDKYKAEYFFPSAKWVGAVRNVASDLGCKFVIL
                     TTKHGLVNPSDDIDHYDLEIKGNENIINEKWQDTTSRLIRRGQYDILVFYAGGCPRDE
                     YLRILSPILKNLEIDLLTFGKPNMGDAGKIKDIVESLGEEASIDTLKKKLKFPDRLLF
                     FPHNNKA"
     gene            6052..6648
                     /locus_tag="PLG98_07030"
     CDS             6052..6648
                     /locus_tag="PLG98_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOE33046.1"
                     /translation="MLSKNMAELQELSRKTCFTLEDVAQSLGMQTASARVLCSRYVRQ
                     GVLIRLKKGFYTTAWKWENLNRQDFFKIANILQVPSYISLMSALAYYEVTTQAQNNYQ
                     ENICLKRSIVYNVREAVFNYVKVQARYYGDFVKTDGIFIATKEKAFVDAAYLYSFGKY
                     KFDVDSLDMKKLDIKKLRSIIKIYPQKTKETVKRLCWI"
     gene            6636..7298
                     /locus_tag="PLG98_07035"
     CDS             6636..7298
                     /locus_tag="PLG98_07035"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE33047.1"
                     /translation="MLDLIQHEQFELEVLNRLQSGRFLANLIFGGGTMLRLCHGLNRY
                     SVDLDFWVVREMDWAKYYSRMEKYLGQFYKIADSANKHFTILFELKSPQFPRSLKIEI
                     RKTVKNVKTETSIAYSPHAPVQVMLKTVALKDMMTSKIEAFLDRREIRDAYDIEFLVK
                     RGIVLNADHEVITQMLTKLGKLGKKDFSVKLGSLLDVSERKYYVENGFRILEANLRDK
                     LR"
     gene            7360..8100
                     /locus_tag="PLG98_07040"
     CDS             7360..8100
                     /locus_tag="PLG98_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOE33048.1"
                     /translation="MNETKFCALILAAGKGTRMKSDKAKVLHELNGKPLLYYSIEAAR
                     AVGVEKIVAVIGHQAERVRETFAANGCIFVEQNPQLGTGHAVLQAKDVLTDYKGFTVI
                     LCGDVPLLKPATIKSLVDNHVAEKAVVSVLTTIPPHPHAYGRIVKDSKGNVLKIVEHR
                     DATEEEKKIGEINTGIYCVDTEFLFHALGKVTNNNRQQEYYLTDIVEIAVCEGQKVKS
                     FIADDYVEVMGINTLEELTRAEKYLRKG"
     gene            8315..9304
                     /gene="dusB"
                     /locus_tag="PLG98_07045"
     CDS             8315..9304
                     /gene="dusB"
                     /locus_tag="PLG98_07045"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOE33049.1"
                     /translation="MKNINNIIEAVAKKGALLAPLAGISDLPFRLIARSQGCSLAYTE
                     MISSNGLVRNTRKTYEYLQTCPEDRPLGAQIFGADPQVMAGAARIVADTGVDLIDINM
                     GCPVKKVIKTGAGAILMKDPDLAARIIKAVKKAVIIPVTVKIRSGWNQSSINAVQIAK
                     IAEDSGADAITIHGRTADQGYSGHADWKIIAEVKKAVKTLVIGNGDIRQPQDAVQMLA
                     ETDCDAVMVGRAALGNPWIFKGISQALRGGEIECHPSLNQRREMIENHWKMEARMLGV
                     KLAGKSFRKHILWYTKGLDNSHRFREMAGKVNNEQEIFEALNNYFHLLNTEKI"
     gene            9360..9635
                     /gene="zapB"
                     /locus_tag="PLG98_07050"
     CDS             9360..9635
                     /gene="zapB"
                     /locus_tag="PLG98_07050"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HOE33050.1"
                     /translation="MENHTLFNEGKVNSTVELEKFSKLEEKIKAIVEEKVSLKNQIKT
                     LEDTLKNKEAEIEKLNGKIKVLDEERNSVRARVDSLLDLIADVNSAK"
     gene            9654..9932
                     /locus_tag="PLG98_07055"
     CDS             9654..9932
                     /locus_tag="PLG98_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOE33051.1"
                     /translation="MKNSYNITILGQELSVLSDSEDEQVAEVVQFLNKKVDEILHSGN
                     GLKTLNVAILAALNISEEFLKLKKVNKELCDKLENRAEELIQLIEETS"
     gene            9946..10143
                     /gene="ssrS"
                     /locus_tag="PLG98_07060"
     ncRNA           9946..10143
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLG98_07060"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10347..11915
                     /gene="rny"
                     /locus_tag="PLG98_07065"
     CDS             10347..11915
                     /gene="rny"
                     /locus_tag="PLG98_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOE33052.1"
                     /translation="MFDASFLSVLVLVVAVVSGIIIGFILKQSIAAKEIRSSKNLMAR
                     MAEEAKKEAETIKKEAVLQAKENLLKMKAEFDAESKERRNDFEAREKRIRTKEENLDK
                     KTDALAQKESSVESREKSISSKEQSLEDKRRKLDAALHEQQERLEKIAGISPEEAKNL
                     LIKSMEAEAKQDAAALVRKIEDDAKLTADKKAQEIISYAIQRYAGDVVAEKTVSAVSL
                     PSEEMKGRIIGREGRNIRAIEAATGIDLIIDDTPEAVVLSSFDPVRREVARISLERLI
                     QDGRIHPGRIEDIVKKVRTEVEQIVRETGEKASFDAGVHDIHPEIINLLGSLKYRTSY
                     SQNVLQHSIDVAYLTGIMASELKMNVREAKRAGLLHDIGKAIDHKVEGTHAAIGADYA
                     KRFGENPRIVQAIATHHDDGRNNTLLGVLVQAADTLSAARPGARREMLETYVKRLEEL
                     EKIANSFNGVDKCFAIQAGREIRILVENEKISENDATMLCKDIVKKIENELTYPGQIK
                     VTVIRETRVSDFAK"
     gene            11946..12734
                     /locus_tag="PLG98_07070"
     CDS             11946..12734
                     /locus_tag="PLG98_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOE33053.1"
                     /translation="MKILFIGDIVGSPGRMAVRELLPDLKARKKIDFVIANCENGAAG
                     FGVTKKIMEELFRDDIDVLTSGNHIWNKREVLEFIGESDRLLRPANYPATAPGAGAVL
                     VPLKTGGYVGVLNLIGRIFMQAVDCPFITAKNKVAELREKTKVIIVDMHAEATSEKKA
                     LGWYLDGEVSAVLGTHTHVQTADEEILPNGTAYISDAGMTGPFDSVIGIRKEAIIERF
                     LTQMPNKFDLAKDDLRMQGVIMDIDVDSGKAKSIDRISVKLKDS"
     gene            12748..14031
                     /gene="tyrS"
                     /locus_tag="PLG98_07075"
     CDS             12748..14031
                     /gene="tyrS"
                     /locus_tag="PLG98_07075"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOE33054.1"
                     /translation="MKNAYDILKERGFVEQITDEGLIQELFAKGPVTCYIGFDPTATS
                     LHIGSLVPIMSLAHMQQSGNKPIALVGGGTGLIGDPSGKTEMRQVLTREQIDYNANCI
                     KQQLSKYLDFSDGKALLLNNADWLTKLNYIDFLRDVGRHFSVNRMLAAESYKIRMEKG
                     LNFIEFNYMVLQAYDFFHLFQNYGCALQMGGNDQWGNMLAGTELIRKIAAKDAHAVTF
                     PLITTSLGQKMGKTEKGTVWLDGALTSPYEYYQYWVNCDDADVERFLKLFTFLPLDEI
                     SAVKNLKDAQLNMAKTVLAFEATKVTHGAKAAEDAWRASAEAFHSRPVETGMFPSCNI
                     PRTAAASDTSAIPRYKIPRKDLKSGVQICGALAKAALTQSISETKRLIEQGGIYINDR
                     QVKSIDEKLTLADFGDAEEIRVRKGKKKYLIVEIE"
     gene            14083..16173
                     /locus_tag="PLG98_07080"
     CDS             14083..16173
                     /locus_tag="PLG98_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE33055.1"
                     /translation="MQKKIERKKKPFHYRHANLRKKSKSNSHAAFYRPEIVPALKSTL
                     SRIGKPYPAPFVPDEFQVKALEAIKKTDCLVIAPTGSGKTWIAREAILSVLEKGGRAW
                     YASPLKALSNSKWIEFGLHFDTENVGIITGDTKENTEAPIIVGTTEILRNQLYDAMHQ
                     GKDLNCDLVILDEAHFLGDTDRGVVWEEIMIYLPARIHLLLLSATIGNGEEIAHWLES
                     IRKKSCAVVAEDKRPVPLYPLFLHPSGCLHPFLEGKKVSPPVADFIRRTDEKRLRGGK
                     MPDYIGIIRVLEKFNLLPAIFFLKSRAECDAALTARGMLQPLQNATVFSYSLDELLGR
                     FPTLARHRQLKVLRSMGLASHHGGQLPAWKMLVEEMMNRGHLRVIFATSTIAAGVNFP
                     ARTIVLFNSDLFNGSDFSPLTATEFRQMTGRAGRRGQDNIGFMLTVTGKFMNLNHIRG
                     LLFQKPEDILSRLKNDFAMVLNLLLSQTPDDVRKIFERSLAAYQQNSRHESADFSAAQ
                     SLWKDFSRHLKFLKQEGFVDESGALTDDGCWASKLRLDYPLLVAQCLRENAFPQDNEK
                     ILAALVALLAYDRDNDMKMTVKDLPPKLALAFKKVMLAVRPLIHRMENAGFAPARLHT
                     LAGVAMYLWAQGYGWDSVIKATGIAEGDMATLVLRTADNLRQIASLKDTYPDIAGCAQ
                     RARETILREPVLFL"
     gene            16532..16849
                     /locus_tag="PLG98_07085"
     CDS             16532..16849
                     /locus_tag="PLG98_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33056.1"
                     /translation="MWLYLKTGFYSVVTKEPCKEDELLVRAHSKDDLDKLQDLLKKQC
                     QFHGEVLDTPQTDYAYRMIVPKETFASFMSRAINDLVYEEYKNTKSWEAAYRWQSNLQ
                     RSK"
     gene            16920..18899
                     /gene="uvrB"
                     /locus_tag="PLG98_07090"
     CDS             16920..18899
                     /gene="uvrB"
                     /locus_tag="PLG98_07090"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOE33057.1"
                     /translation="MITDFVPAGDQPQAIDRLVQGLEKGNEHQVLLGVTGSGKTFTIA
                     NVIERVQRPALIIAHNKTLAAQLYEEFKGLFPENAVEYFVSYYDYYQPEAYLPVTDTY
                     IEKDSAINEEIDKLRHAATHALLTRRDVIIVASVSCIYGLGSPEAYLGMIVQLEAGKE
                     FSRDELLKRLIEIQYERNDIDFHRGTFRVRGDIVEIFPPYEDEQALRIEFFGDMIESM
                     TLIDPLRSKKIGVIHKTAVYPGSHYVSSRENLQRAIHDIRRELALTLAEYRDQNKLLE
                     TQRLEQRTNFDLEMMEEMGYCQGIENYSRHLTGRKPGQPPPTLMEYLPQDALIIIDES
                     HATLPQLAGMYRGDRSRKETLVKYGFRLPSALDNRPLRFDEYEALPNQRIYVSATPAQ
                     YEMAKAKGIRVEQIIRPTGLMDPEIEVKPATNQVDDLLAEIRARIEKNEKVLVTTLTK
                     RMAENLTDYYAGLDIKVRYLHSDVHTLERVDIIRDLRRGVFDVLIGVNLLREGLDIPE
                     VSLVAILDADKEGFLRSERSLIQTSGRAARNVSGKVIMYADKMTHSIQACLDETKRRR
                     AIQQKYNEENNITPETIKKSISSVLGSIYEADYVTLPATVSERVAPLKEEDLPAFIAK
                     LTREMKQAAKNMEFEHAAELRDEIRELNKILLEMG"
     gene            19014..>19392
                     /locus_tag="PLG98_07095"
     CDS             19014..>19392
                     /locus_tag="PLG98_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33058.1"
                     /translation="MKALKSILCVLTLFVFVFAGCEKEASDWVQYHKDADGNVYWYDQ
                     NRVKVDAANYTVEVWAKQVYSDMGRTIELQSRIKDGLSMKGYDKLAHKTCRYAIDCGK
                     KGITVLSISHFDSDGESLYFDVDK"
     assembly_gap    19393..19492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19724..20122
                     /locus_tag="PLG98_07100"
     CDS             19724..20122
                     /locus_tag="PLG98_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33059.1"
                     /translation="MNKVVARLANGKMIKGTTADFFPGKDVFHVTESAAAPGTKALEV
                     FTKDMKALFFVKDLAGNPQHVKTNEFNASQPVVGRKIKVVFKDGEVLIGTTTGYQPGR
                     PGFFLVPADAGSNNERCYVVTASAKEISFL"
     gene            20201..20437
                     /locus_tag="PLG98_07105"
     CDS             20201..20437
                     /locus_tag="PLG98_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HOE33060.1"
                     /translation="MPQKQKKKKRDIEKDYTAGQFVMKLRRLADCIETGHPFRIQIAG
                     EKITIPTDAVINIEHERGTSEEEVEFQIKWKLNR"
     gene            20537..21520
                     /locus_tag="PLG98_07110"
     CDS             20537..21520
                     /locus_tag="PLG98_07110"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HOE33061.1"
                     /translation="MLDDIYHGYLDWNMMFAAERLGRLDAQKTGIAAYLARDSYYNDP
                     RYAETLLTLGFFDVQLFYHNHLQALIGCRGIQTPEGAKALVVIVFRGSDQVQDWLQNL
                     DIRMTRFLNKEKFPHFSRLKVHNGIFERVHDFEDWSRRVIFKDGPLKGNLLQMLGDEL
                     KRKQCFFWIIGHSLGGAMATLYAARLCDCYNVSRNRILSHTFGAPPIGNRYFAARYGH
                     ESCLRAQKIKRKSDSLALFRFVNTEDPIPAPRFADVRVEKAQGGPIAQPPYRLLGFKH
                     VGKEVRFSPRHLPGFYEKYKQFTGRRYNSSHFMKVHFMEAYITGIDVLNTH"
BASE COUNT         6148 a         4563 c         5446 g         5417 t
ORIGIN      
        1 cgctcaagac aaagaagaga ctgacgccgg cgtcgccgcc gttgattaaa aacggcgcaa
       61 gccattcggg aattgcgaga ttaggttgag gaatcaggat caggtgactg atgaccacat
      121 aaatgattgc gaaacagcgc agggaatcca gaaagccgag gcggacatag ggcgtggtcg
      181 tagaattgtt tggcattaaa cgctccgtaa aattggagta ttccggcaaa agaaataaaa
      241 aactctttaa gcgtcttttc gtatcttctt catgtgatcg atacgtttct ggatgagcgc
      301 gtcggtgccg atgtcttcgc gataagcaac gggacctttg acggccttta ccccttcctc
      361 ggcaattttc cgggcatccg ccagattatc ggcaattccg acaatgccga tggcgcgtga
      421 agagctcaaa tacaagccgt catccttctg atccacagat gaataatata gcctggccgg
      481 ccccacattc cccacttcaa ttttcgccct ggatgaggcg gcatcgggat ggtcggcggg
      541 caggccgtag cccttcggca caacatattt gcaaaccgta gccttcttat caaactctat
      601 ttctatttga tccagagttc ctgcaatgat gtgccggcac acatccacga aatcggtttt
      661 gagaagcggc agaatattca tggcttccgg atcgccgaac cgtgcgttgt attccaagag
      721 cttgacgccg tccttcgtgg cgatgaatcc tccatacatg acgcctttgt agggactgcc
      781 cgtttctttc aaaagtgctt ctgcgacctg acgggtgatt tccagtccgt catgcagatc
      841 ctgcggtctc aaaaaaggca tcgaatggtc cgccagtgaa taagagccca tgcctccggt
      901 attggggccc ttgtcaccgt caaatcttct cttgtgatcc tgcacgagcg gagtagcgac
      961 aactgtcttc ccgtcgcaca ggcattgcag cgaaaattct tccccgtcga acttttcttc
     1021 aatcaccagc gaagcgcctc cggccagtat ctgtgcgcat agtttcaacg cttctttttt
     1081 tgaatcaaaa tgatctccct ggacctgaac gcccttgccg cccgtcagtc cgtccggttt
     1141 caaaacgatg ccttccaact gattgagaaa atcttcgacg ccatcgagcg ttttaaatac
     1201 tttaaactgc ggattaccgg gaatattgta tttgctgact agattcctgg tgaacgattt
     1261 ggacgtttcc agccgggcca gcgattttgt cggtcctacg gcgggaatat ttattttggc
     1321 cagcgcgtcc gcaacgccgt tatttaaagg atcttccggc ccgattacgg caaattcgat
     1381 tttattttcc gtggcaaatt ttgtaatcgc gtcaagatcg gcataagaac caatcaaggt
     1441 tttttctgaa agagaagcga tgcctgggtt attggatttc atgaaagaga ataagctggg
     1501 cttttgtccg gagcgtgtaa tcgcttcagc cagtgcgtgt tcgcgggcgc cgtttccgag
     1561 cagtaatata ttgggcatgg ggatctcctg tcgggctttt ggatttttcg cttttataaa
     1621 gagaatagat tgggctgtca atggaatatt caaacgaagg caatgtttct ttttttgaca
     1681 gaatctgtta aattaataac caaggttaga aatcttggta taacttctct tgggtcattt
     1741 cggggagcaa agcgacggga aatctacatt caatattatt gccggatgat agtttttggt
     1801 cgaaaagaca gtttgatagt tatgcaaaag tttcaattca tgagatgagg tgtctgggta
     1861 ttgggctgga attccgacat agttgtttta atccttcttt tcacgctttc cggatttttt
     1921 tcgtcatcgg aagccgcttt gctttcactg cgggatttgc atctgcataa gatgcaacaa
     1981 aacaattacc ctttttttaa ttacgtcgtc aaactgcttt ccaaccagcg cnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacaaaacaa ttaccctttt tttaattacg
     2161 tcgtcaaact gctttccaac cagcgccggt tgttgattac aatcgtaacc agcaatgaag
     2221 tggtcaacgt cagcatttcc atcctggcgg cgtctttttt tatcgggctt ttcggtagca
     2281 gcgggcaatg ggtgtcgatt gtttttacaa ccgtcgtttt gctttttgtc ggcgaagcta
     2341 ttcccaaaac ttttggcgta acctacccga tgcagatttc ctcgctggtg tctccgctct
     2401 tgatggccat ttcgcgaatc gagtctccta tcgtttcagc cctggaaaaa gtatccggtc
     2461 ttttcctgcg gcgttacggg gttaataaat ccgaggaatc ggaagtcatt atggaacacg
     2521 aattcaaaac gcttgtcgat gtcggaagtc tcgaaggcgt tgtgaatgag gcccagaaag
     2581 aattgatcaa taaaatattc gaactcggag acaggccggt gacggatatc atggtgcccc
     2641 gtgtggacat gttttgtctt ccgctggata tgccggtcgg ggagatgctt caatccatcg
     2701 ccgccgcccg gcaggaacgg gttccggttt atcgggagga tagagacgat atcgtcggaa
     2761 tcctgtttgc gagggatttg ctcaataatg tctggcgcgg cagatctcag accggtattt
     2821 acaatctttt ggtcagaccg tttttcgtgc ccgaacaaaa aacaatcaat ggtctgctgc
     2881 acgattttca gaaaaatttt attcagatcg ccattgtggt ggacgaatat ggcggcgttt
     2941 ccggcatgat tacgctggag catattctgg aacatctttt tgaagagccg gaaggtgatg
     3001 aggaaatggt ttgcgacggt tgtgaaaaaa tcgacgagca atcggttatt gtccccggga
     3061 gtatgtcggt cgagcagtgc aataagcttc tggaagcgga attgccggaa gacgagtttg
     3121 acaccgtcgg cggactggtg cttcatcttt tcggtaaaat accgcagaag ggcgaagagg
     3181 cgcatctgga cggatatatc ttttgcgtgc atgatgtcgg caaaacaaga atattgaagg
     3241 tcaggattac gaaaaaaaca gttgaggaac ctgaagagtc atgagcctat atttaatcgt
     3301 cgcagccata ttgttttgtc tgatgctgga aatgctttat tccggcggtg aagtagcgct
     3361 ttttgcttcc gatatcaata aactgaaaaa ccgagcgcat caggggtcaa catccgccca
     3421 ccaggccgtc aagctcaaag aaaagccgga atggtttatc tcgacggcac ttgtcgggac
     3481 caatctggcg atcatcatcg cttcaacgct tatcaccggt cttttaattt ctttttacgg
     3541 tccggcacgc ggcgaaaaaa tcgctttcct gatcatgatt cccacgctgt ttctcatgat
     3601 cattgcccgc agcatatttc tttactacgc ggaagccatg gcgatgcgtg ttgcctggtt
     3661 tatccgtttt tcatcgatac ttttttatcc gatcgccttc gtgatcgcgg ccgtttccag
     3721 aagcacggtg catttctctt ccgataccag aagcgggcag tcctcgcaca ttaccaagga
     3781 aggcctcaaa tacatcttgg gcgaaaaaac aaaagggggc gatatcctga cgcgtgagaa
     3841 ggaaatggtc agccgggtgt ttgatttctc cgaacttacg gccatgaaaa tcatggtgcc
     3901 ggtttccgct ttgacggcgc tgtccgcttc catgaaaatt tccgaagccg taggtgttgt
     3961 ggccgataaa aaatacatgc gcattcccgt ttatgaagat accgtttaca acatcgtcgg
     4021 cattttacgc tacttcgatc tttttgaaat gctcctcaag gacaaggaga aaggcgaagg
     4081 tgtcggcgaa gcgacggttg cctcctgcat gcgcaaagat gtcttttatg ttccggaaac
     4141 aaaaaaggtc agcgaactgc tcgcagcaat gcaaaaaaga gacgagcaga tggcggtggt
     4201 ggtggatgaa tacggtggcg cgaccggcgt tgtcaccatt gaggatatcg gcgaagaaat
     4261 cgtcggaaca attgatgaat acgtgtcggg agaaaagctt tacagaaaaa ttgctgaagg
     4321 acgttatctt gtgtcaggca ggctggaaat aggaactctg cggcaattgg ttaaagcgaa
     4381 attccccgaa gggaattatg aaaccgtagg cggctttcta atgtatcaga tgggtaaaat
     4441 tccccgaata aaagaatatt atcagtatgg cgatgtgacg tttattattg aaaacgccga
     4501 ccagaaatcg attaaggaag tgacggtaat cgcgcctttt attcaagagg ggaacaaata
     4561 aatatctttt tgtcattccc gcgcaggcgg gaatccagta aatgtttttg ttcaaaaaag
     4621 attattaata attggagtga ttttgataag tgtagatgag ataatcaagg attattaaat
     4681 gaaatcagat ttaacaagac aagattggga agagtattta atccgtctct attttgggac
     4741 caaaaatgat ttaataaaat catgtatcaa tagagcctat cgcgatttca atagaaccct
     4801 tcatggaatg gatgatcaca aaaataaaca agatttattt gatagagccg taaaatatct
     4861 gctctcaatt ttcatagata taaaaaaaga gtcattaaat gaccacgaag aatttgataa
     4921 atggcataaa gaaaaatgcc ttcatttaaa aaatttatat aaagaaaatg atttcattaa
     4981 tttttccatt gggcaggcac agaaatggat aaacatgact tttaagtata tattttgtgt
     5041 gggtgagaaa agattacccg gttattctgg tttatatgag ttttgtcatg ttcctattga
     5101 caatataatt ttagataaaa ttaaaaaaag aggtgctcct gtcccgtcaa aaccgtggag
     5161 tcgacttggt gattataacg aatatatctc tctccagaag tggttccgag aacaatataa
     5221 attaccattg gatggtgaat ttatattatg gatggaggaa cctcatgata atgtcattat
     5281 gcctgttcaa aacaatattg ttttggataa aaccagagtc aaagaagcaa aagacgagac
     5341 cataggacca tatcaactta acagaataat tctgtgctct gccgatgcga gtccggcatc
     5401 acaaaagaaa gacaaatata aagcagaata tttcttccca agcgcaaagt gggttggagc
     5461 cgttagaaat gtcgcaagtg atttgggatg taaatttgtt attcttacga ccaaacatgg
     5521 gctggttaat ccttccgatg atattgatca ttacgatcta gaaatcaaag gtaatgaaaa
     5581 tattattaat gagaaatggc aagatacaac gtcaagatta attagaagag gacaatatga
     5641 cattctagtt ttttacgcag gtggatgtcc tcgggatgaa tacttgagaa tattaagtcc
     5701 gattcttaag aaccttgaaa tagatttatt aacgtttggg aagcccaaca tgggagatgc
     5761 gggaaaaatt aaagacatcg tcgaatcgtt gggggaagaa gcatctatag atacattgaa
     5821 aaagaaactt aaattcccag atagactatt atttttcccc cacaataata aggcataatt
     5881 aattttaaaa tactggatac cggcctgcgc cggtatgaca aacgaataag gattgataag
     5941 gtgtaggaaa gtgcgttgat attggcggcg gggaagggta cccgcatgaa ataaatccgg
     6001 gcttgcaaaa ttttgcaaag tatatataat taacaaaagt gataactata tatgcttagt
     6061 aaaaatatgg cagaattgca ggaattgagc aggaaaacct gctttaccct cgaagatgtg
     6121 gcgcagagtt tgggaatgca aacggcatcc gcccgcgtat tgtgcagccg ctatgtccgg
     6181 cagggggtgc tgataagatt gaaaaaaggt ttttatacca ccgcctggaa gtgggaaaat
     6241 cttaatcgtc aggatttttt caaaatcgcc aatatcctgc aggttccttc ctatatttcc
     6301 ctgatgtcgg ctctggctta ttatgaagta acgacgcagg cgcagaacaa ctatcaggaa
     6361 aatatttgcc tgaaacgttc gattgtttac aatgtaaggg aagcggtctt caattatgtc
     6421 aaagttcagg cccgatacta tggtgatttt gttaaaacgg atggcatctt catcgccact
     6481 aaagagaaag cgtttgtgga cgctgcctat ctttattcgt tcggcaaata taagttcgat
     6541 gttgattctc tggatatgaa gaagctggat atcaagaagc tcaggagcat catcaaaatc
     6601 tatccgcaaa aaacaaaaga gacggttaaa agattatgct ggatttgatt caacatgaac
     6661 aatttgagct ggaagtttta aacagacttc aaagcgggcg gtttctggcc aacttgattt
     6721 tcggcggcgg caccatgttg cgcttgtgtc atggactgaa tcgttattca gttgatctgg
     6781 atttctgggt agttagggag atggattggg caaaatacta tagccggatg gaaaaatatc
     6841 tgggccagtt ttacaaaata gccgattccg ccaataagca ttttacaatt ctgttcgaat
     6901 tgaaatcgcc gcagtttcca cggtcgctga aaattgagat acggaaaacg gtaaagaacg
     6961 tcaagacaga aacgagcatc gcctacagtc cgcacgcgcc ggttcaggtg atgttgaaaa
     7021 ccgtggcgct aaaagatatg atgacttcaa aaatcgaagc ctttcttgac cgccgggaaa
     7081 tacgcgacgc atatgatata gaatttctag taaaacgcgg gattgttttg aatgccgatc
     7141 atgaggttat cacccaaatg cttacgaaac tggggaaatt gggcaaaaag gatttttcag
     7201 taaagctagg ttcgttgctt gatgtctcgg aacgaaagta ttatgtggaa aacggattcc
     7261 gcattctgga ggcgaacctg agggataaac tcaggtagaa tttaaaatac aggataccga
     7321 ccttcgttgg tatgacgatt gaaggagatg tttgataagg tgaacgaaac gaaattttgt
     7381 gcgttgatac tggcggcggg gaagggtacc cgaatgaaat cggataaggc caaggttctg
     7441 catgagttga acggaaagcc cctgctttat tattccatcg aagcggcaag agcggttggc
     7501 gtcgaaaaga ttgtagccgt gatcggccat caggcggaga gagtcaggga aacatttgcc
     7561 gccaacggct gtatttttgt cgaacagaat ccgcagttgg gaacggggca tgccgtttta
     7621 caggcgaagg atgtactgac tgattacaaa ggctttaccg taattctttg cggcgatgtg
     7681 ccgcttttaa aaccggcgac aatcaaatcg ctggtggata atcatgtcgc cgaaaaagcg
     7741 gtcgtttctg tgttgacaac cattccaccg catcctcatg cctatggccg catcgtaaag
     7801 gacagcaagg gcaatgtttt gaaaatagtc gagcataggg atgccacgga agaggagaaa
     7861 aaaatcgggg aaatcaatac cggcatttat tgtgtggata cagagttttt gtttcatgct
     7921 ttgggaaaag tgaccaataa caaccggcaa caggaatatt atctgaccga tattgttgaa
     7981 atagccgttt gtgaaggcca gaaggtaaaa tcatttatcg ctgacgatta cgttgaagtg
     8041 atgggaatca acacgctgga agaactcacc cgcgcggaaa agtatctccg aaaaggctga
     8101 aaattttctg ttttatttgt ttctgtcctg atattaacat taaagtgaaa caattatttt
     8161 tcggtcatac cggtcgcaga tgaccttcag gttacgaagg ccggccatgg tgcccctata
     8221 gggtatatcc agttattctt ggatgccgtt cttcaacggc atgacatagt tgtgcgtgat
     8281 attttatttt tcagtcagct aggtaattat tggtttgaaa aatataaaca acataatcga
     8341 agcagtcgca aaaaaaggag ctctgcttgc gcctctggcg ggaatttccg atttaccttt
     8401 ccgtttaatc gcccgatctc aaggatgttc cctggcctat acggagatga tcagttccaa
     8461 tggtcttgtc cgaaacacca gaaaaacgta cgagtatctt caaacctgtc cggaggaccg
     8521 cccgctcgga gcgcaaattt ttggcgctga tccacaggtc atggctgggg cagcgaggat
     8581 cgtggcggat accggggtgg atttaattga tatcaacatg ggctgtccgg tgaaaaaagt
     8641 gattaaaacc ggtgccggag ctatcttgat gaaagatccc gatcttgctg cccggataat
     8701 taaagcggta aaaaaagccg ttattattcc ggttacggta aagattcgtt caggatggaa
     8761 tcagtcgtct atcaatgctg tgcaaattgc aaagatagcg gaggattcgg gagccgacgc
     8821 aattaccata catggccgca cggctgacca gggttacagc ggtcatgccg attggaaaat
     8881 catcgcggaa gtcaaaaaag cggttaaaac actggtcatt ggtaatgggg acatccgtca
     8941 accgcaggat gcagtccaaa tgcttgctga aactgactgc gacgcggtga tggtggggag
     9001 ggctgcattg ggcaatccat ggattttcaa aggaatttct caagcactgc ggggaggcga
     9061 aattgaatgc catccctcgc tgaatcaacg tcgggaaatg attgaaaatc attggaaaat
     9121 ggaagcccgg atgctcggtg tcaaactggc cggaaaaagt tttcgtaagc atattctctg
     9181 gtataccaaa ggtttggaca attcacaccg gttccgggaa atggccggca aggttaataa
     9241 cgaacaagaa atctttgagg cactaaataa ttattttcat ttgctgaata cggaaaaaat
     9301 ctaaaataag gacttgacat taatgcgata aattggcata aaataaggaa aaagaaacta
     9361 tggaaaatca tactttattt aatgaaggaa aggtaaattc tactgtggaa ctcgaaaaat
     9421 tttcaaagct cgaagagaaa attaaagcta ttgttgaaga gaaagtttct ttaaaaaatc
     9481 aaattaagac attggaggat acattaaaaa ataaagaggc ggaaatagaa aagttgaacg
     9541 gaaagataaa ggtattggat gaggaaagga acagcgtacg cgcgagggta gattcactgc
     9601 ttgatttaat tgcagatgta aattcagcaa aataagcttc cagggcgtgt gttttgaaaa
     9661 acagttacaa tataacaata ttaggccagg aactttctgt tttgagcgat tcggaggatg
     9721 agcaggtggc cgaagttgtt cagtttttaa ataaaaaggt ggatgagata ttacattcgg
     9781 gcaacggttt gaagacattg aacgttgcca tcctggcagc tttgaacatt tcagaggagt
     9841 ttttaaagtt aaaaaaagtt aacaaagagc tctgtgacaa attggaaaac agggccgaag
     9901 aattaattca gcttatagag gaaacaagtt agaaactttt tttattagcc cctgcgatgt
     9961 gcgtgattga catttgtttt tgagccaaca ttttgacctt gggaccttat ccttgattgt
    10021 ggtgtgcatg tccgcttttt cctgagctgg tcgggaagca gaaagcctga aacaaccaat
    10081 accggttccc accttgttaa aaggttcaaa aaaggtgatt aacacggcat aggcgggggt
    10141 cttttttatc tgttcatcaa catcaattct ttccagcctt tagaaaatca ataaaaacgc
    10201 caaacaccga ggtgaagcat ctgaaccatt gatgcgcgcc gtggttaaat atagctcaac
    10261 cggaaaagaa gtttatattc ttgaaattga ctgtgatcag gtcggggaat gaggtttccg
    10321 gaagggcacc aaggaggtaa catgatatgt ttgatgccag ttttttatca gtgctggttt
    10381 tagttgtagc ggttgtttca gggatcatta ttggcttcat tttgaagcag tccattgcgg
    10441 ccaaagaaat cagatcatcc aagaatttga tggctcgcat ggcggaagag gccaagaaag
    10501 aggctgaaac aatcaagaag gaagcggttc ttcaggccaa ggaaaatctc ctgaagatga
    10561 aagccgaatt tgatgctgaa tcgaaggaaa gaagaaacga tttcgaagcg cgtgaaaaac
    10621 gcattcgcac caaggaagaa aatctggata aaaaaaccga tgctctggcg caaaaggagt
    10681 cgtcggttga atccagggaa aaatcaatca gcagtaaaga gcaatccctt gaagacaaac
    10741 gtcgtaaact ggatgcagcg cttcatgaac aacaggagag actcgaaaag atagcgggta
    10801 tctctcccga ggaagcgaaa aacctgctga ttaaatccat ggaagcggaa gccaaacagg
    10861 atgccgcagc gcttgtccgc aagattgaag acgatgccaa gctgaccgcc gacaaaaaag
    10921 cacaggaaat tatttcctat gcgattcagc ggtatgccgg cgatgtagtg gcggaaaaaa
    10981 cagtttcggc ggtcagtctg cccagcgaag aaatgaaagg gcggatcatc ggccgtgagg
    11041 gccgcaatat ccgcgccatt gaagcggcca cgggaattga tttgatcatt gacgatacgc
    11101 cggaagcggt tgttttgtcg agtttcgatc cggtcagacg cgaggtggcg agaatttcgc
    11161 tagagcgcct gattcaggac ggacggattc atcccggccg tatcgaagat atcgtcaaga
    11221 aagtcaggac agaggtcgag cagatcgtca gggaaacggg tgaaaaggca tcctttgacg
    11281 ccggtgtgca cgacatccat ccggaaatca tcaatctgct gggatcgctg aagtaccgta
    11341 ccagttattc tcagaatgtt ttgcagcatt ctattgatgt tgcctatttg accggtatca
    11401 tggcgtcgga actgaaaatg aatgtccggg aagccaagcg tgccggtctt ttgcacgata
    11461 tcggcaaggc aatagaccat aaagtcgaag gcacgcatgc agccatcggc gccgattacg
    11521 ccaagcgttt cggtgaaaat ccgcgtattg ttcaggcgat agcaacgcat catgatgacg
    11581 gacgcaacaa taccctgctc ggcgttctcg tacaggcggc ggatacgctt tctgctgcca
    11641 gaccgggagc gcgtcgcgag atgctcgaaa catacgtcaa acgtctggaa gagctggaaa
    11701 aaattgccaa ttccttcaat ggtgtcgata aatgctttgc catccaggcc ggtcgtgaaa
    11761 tccgtattct tgtggaaaat gaaaaaatat ccgaaaatga tgccacgatg ctgtgtaagg
    11821 atatcgtcaa gaaaattgaa aacgaactta cttatccggg ccagatcaag gtgaccgtca
    11881 tcagggaaac aagagtttct gattttgcca agtaattttt ccggtatggg aaaggaacga
    11941 tgcaaatgaa aattttattt atcggtgaca ttgtgggcag ccccggccgc atggcggtca
    12001 gggaactgtt acccgacctg aaggccagaa aaaaaattga ctttgtcatc gccaattgtg
    12061 aaaatggagc cgccggattc ggggtgacca agaaaatcat ggaagaactc ttcagggatg
    12121 acattgacgt gctgacatcc ggcaatcata tctggaacaa aagagaagtc ctggagttta
    12181 tcggagaatc cgaccggctt ttacggccgg ccaattatcc ggccacggcg ccgggcgcgg
    12241 gagcggttct tgtccctttg aaaacgggcg gctatgtggg tgtcctcaac ctgatcggac
    12301 ggatctttat gcaggcggtc gattgtcctt ttatcacggc gaaaaacaaa gttgcggaat
    12361 tgagagaaaa aacaaaagtc attattgtag acatgcatgc ggaggcaacc tccgagaaaa
    12421 aagcgctggg ctggtatctt gacggcgagg tatccgcagt cttggggacc catacgcatg
    12481 tgcagaccgc tgacgaggaa attttgccga acggaacggc gtatatcagt gacgcgggca
    12541 tgacggggcc ttttgattcc gtcatcggta tccggaaaga agctattatc gagcgatttt
    12601 taacccagat gccgaacaag ttcgatttgg caaaagacga tttaagaatg cagggcgtca
    12661 ttatggatat tgatgttgac agcggcaagg caaaatccat tgatagaatc agcgtcaaac
    12721 tgaaggattc gtaagaggct ggatgcaatg aaaaatgctt acgatatttt aaaagaaaga
    12781 ggtttcgtcg agcagatcac cgatgaaggc ttgattcaag aactttttgc gaaaggtccg
    12841 gtgacctgtt acatcggatt cgatcccacg gcgaccagcc tgcatatcgg gtcgctggtg
    12901 cccattatgt ctctggcgca catgcagcaa agcggcaaca aacccatcgc gctggttgga
    12961 ggcggtaccg gcctgatcgg cgatcccagc ggcaaaaccg aaatgcgtca ggttctcacc
    13021 cgcgaacaga tcgactacaa cgccaattgc atcaaacaac agctttccaa atacctcgac
    13081 ttttcggacg gtaaggcact tcttttaaac aatgccgact ggctgacgaa attaaactac
    13141 atcgattttt tgagggatgt cggccgccat ttcagcgtca accggatgct ggcggcggaa
    13201 agctataaaa tcaggatgga aaaaggcctg aacttcatcg aattcaatta catggttctt
    13261 caggcttacg atttctttca tctgttccag aattacggat gtgcgctgca gatgggcggc
    13321 aacgaccagt ggggcaatat gctggccggc accgagctga tccgcaagat tgccgccaaa
    13381 gacgcccatg ctgtgacgtt cccgctcatt accacgtccc tggggcagaa gatggggaag
    13441 accgaaaaag gcacggtctg gctggacggc gctttaacca gtccttatga atattatcag
    13501 tactgggtca attgcgacga cgccgatgtg gaacgatttt taaaactgtt cacgttcctg
    13561 ccgctggacg aaatcagcgc tgtgaaaaat ttaaaggatg ctcaactgaa catggccaag
    13621 acggtgctcg ctttcgaagc gacaaaagtt acgcacggag caaaagccgc tgaagatgcg
    13681 tggcgcgcgt ccgcggaggc atttcattcc cgtccggtgg agaccggtat gtttccgtcc
    13741 tgcaacattc cccgcacggc agccgccagc gatacatcgg ccatcccccg ctataagatt
    13801 ccccgaaagg atttgaaatc cggcgtccag atatgcggag ccttagccaa agctgcgttg
    13861 acgcaatcca tctccgaaac gaaacgcctg attgaacagg gcggtattta catcaatgac
    13921 cgtcaggtga aatccattga tgaaaaactg acgcttgccg attttggcga tgccgaagaa
    13981 attcgcgtga gaaaaggaaa gaaaaagtat ttaatcgtag aaatcgagta aaaaatattt
    14041 ccttgtgcgg cacagtcctc ttttctcaaa agcataactg aaatgcagaa aaaaattgag
    14101 agaaaaaaga agccctttca ttaccggcac gcgaacctcc gaaagaaatc caaaagcaac
    14161 agccatgctg ctttttacag gcctgaaatc gttcccgcgc tgaaaagcac tttatccagg
    14221 atcggcaagc cgtatccagc cccttttgtt cccgatgaat ttcaggtcaa agctttggag
    14281 gcaatcaaaa aaaccgattg tctggtcatc gcgcccacgg gttcaggaaa aacatggatt
    14341 gcgcgggaag ccattttatc cgtcctggaa aaaggcggac gtgcctggta cgcgtcgcct
    14401 ctgaaagcgt tatccaattc caaatggatt gaattcggcc tgcatttcga tacggaaaat
    14461 gtcggtatca tcaccggcga taccaaagaa aacaccgagg cgccgatcat tgtcggcact
    14521 accgaaatcc tgcgcaatca actctacgat gccatgcatc agggaaaaga cttgaactgc
    14581 gatctggtca ttctggacga ggcgcatttc ctgggcgaca ccgatcgcgg cgttgtctgg
    14641 gaagaaatca tgatttatct tcccgcgcgg attcacctgc tgcttttgtc agcaaccatc
    14701 ggtaacggcg aagaaattgc acactggctg gaatccattc gcaagaaatc ctgcgctgtc
    14761 gttgcggaag acaaacgtcc ggtgccgctt tatccccttt ttctgcatcc atccggttgt
    14821 cttcatcctt ttctggaagg gaaaaaagta tccccgcctg ttgccgattt catcagaaga
    14881 accgatgaga aaagattgcg cggcgggaaa atgccggatt acatcggcat catccgggta
    14941 ctggaaaagt tcaatctgct gccggcaata ttttttctga aatcgcgcgc ggaatgcgat
    15001 gcggcgctca ctgcgcgcgg aatgctgcag ccgcttcaga atgccacggt gttttcatat
    15061 tcactggatg aactgctggg acgtttcccc acgctggcca ggcaccggca attgaaagtt
    15121 ttaagatcga tgggactggc gtctcatcac ggtggacagc tgcccgcgtg gaaaatgctg
    15181 gtggaagaaa tgatgaacag aggacatctg cgcgtgatct ttgcaacatc cacaattgcc
    15241 gcgggcgtta attttccggc tcgaaccatt gtccttttca actccgattt gttcaacggc
    15301 agtgatttca gtccgctgac ggctacagaa ttccgccaga tgaccggacg cgcgggacgc
    15361 cgcgggcagg acaacatcgg cttcatgctg acagttaccg ggaaattcat gaacctgaat
    15421 cacatccggg gtttgttgtt tcaaaaaccg gaggatattt taagccggtt gaaaaacgat
    15481 ttcgcaatgg tcctgaatct gcttttgtcc cagacgccgg acgatgtccg caagatattc
    15541 gagcggtcgc tcgcggcata tcagcaaaac agccggcatg aaagcgcaga cttttccgcg
    15601 gcccagtcgc tctggaaaga tttttcccgc cacctgaaat ttctgaagca ggaaggattt
    15661 gtggatgaat ccggagcgtt gactgatgac ggatgctggg cgtcaaaact gcgtctcgat
    15721 tatccgttat tggtggctca gtgcctgcgg gaaaacgcgt ttcctcagga caatgaaaag
    15781 attctggccg ctcttgtggc gttgttagcc tatgatcggg acaatgatat gaaaatgact
    15841 gtaaaagatt tgccgccgaa gcttgctctt gcgtttaaaa aggtcatgct ggccgtccgg
    15901 cctctgatcc atcggatgga aaacgccgga tttgcgcccg ccagactcca cacgctggcg
    15961 ggcgtggcca tgtatctctg ggcgcaggga tatggctggg attccgtcat taaagccacc
    16021 ggaatcgctg aaggcgatat ggcaacgctg gttttgcgca cggcggataa tctgcggcag
    16081 atcgcatcgc ttaaagatac atatcccgac attgccggat gcgcgcaaag ggcaagagaa
    16141 acgatcctca gggagcccgt tctctttctt taaagagatc tgttgaaaga tcgcattctc
    16201 tgaacgtcgc cacgttagat atcgttttgt atagatcgct tggtccatgt ttcgcagatg
    16261 cgtatgatcc cgctcttgca cgcccatggt gtcagtattt tttgaacagt ccataaaagt
    16321 gaaaaaaatt tcagccgtca cggcagagcg atgaccggtg acgcgtatct tttgattcaa
    16381 ctttcatttt caaaatattg attttatgat aaacttaata cgtttgaatt tgaaattgaa
    16441 atgatcatcc atgattagtg aagatctgaa gacgtttttt taacggacta gcgcgcgtat
    16501 ttgccattga ggaaaaacgc aaggggaaac gatgtggctt tatcttaaaa caggttttta
    16561 ttcggtagtg accaaggaac cgtgtaaaga agatgagctt ttagtcagag cgcacagtaa
    16621 agatgatctg gataaattac aggatttact gaaaaaacag tgtcaatttc acggcgaggt
    16681 gctggatact ccccaaaccg attatgcata ccgtatgatc gttcccaaag aaacatttgc
    16741 gtcatttatg tcccgcgcca tcaatgatct ggtttatgaa gaatacaaaa acacaaaaag
    16801 ctgggaagcc gcttatcgct ggcagagtaa tttgcagcga agtaaataag ccatccgctt
    16861 cttcgcgcag caacaaatcc gatcatttaa aacaatcata gcatatgaag aaactttcaa
    16921 tgataacgga ttttgttccg gccggggacc agcctcaggc gattgatcgt ctggtgcaag
    16981 gtctggaaaa gggcaatgaa catcaggtcc ttctcggggt aaccggttcc ggcaagacgt
    17041 ttacgattgc caatgtcatc gaacgcgttc agcgtccggc gctgattatt gcgcacaata
    17101 aaacactggc ggcgcagttg tatgaagaat tcaagggact cttccctgaa aacgccgtgg
    17161 aatattttgt cagttattat gattactacc agccggaagc ctacctgccg gtaaccgaca
    17221 cgtatatcga aaaggattcg gccatcaacg aggaaatcga taaactgcgc catgcggcaa
    17281 cccatgctct tttgacacgg cgcgatgtta ttatcgtcgc cagcgtttcc tgtatttacg
    17341 gtttgggatc gccggaggcc tatctgggaa tgatcgtcca gctggaagcg ggcaaggaat
    17401 tctcacggga tgaactgctg aaacggctga ttgaaattca gtatgagaga aacgatatcg
    17461 attttcatcg cgggacgttc cgcgtccgcg gcgatatcgt ggagatcttt ccgccttatg
    17521 aagacgaaca ggccttacga atcgaatttt tcggtgatat gatcgaatcg atgacgttga
    17581 ttgatccgtt gcgaagcaaa aagataggcg tcatccataa gactgctgtt tatcccggaa
    17641 gccattatgt ttcttcccgt gaaaatctgc aacgggcgat tcatgacatt cgccgggaac
    17701 tggcactgac gcttgccgaa tacagagacc agaataaact gttggagacg caaaggctgg
    17761 agcagcgcac caacttcgat ctggaaatga tggaggagat ggggtattgt cagggtatcg
    17821 aaaattattc ccgtcattta accggacgca aacccggtca accgccgccg acattgatgg
    17881 aatatctgcc gcaggatgcc ctgattatta ttgatgaaag ccacgcgaca ctgccgcaac
    17941 tggccggtat gtaccgcggc gaccgttccc gcaaggagac gctggtcaaa tatggattcc
    18001 gccttccctc cgcgctggac aaccgtccat tgcgttttga cgaatacgaa gccctgccca
    18061 atcagcgcat ctatgtttcg gcgacgccgg cgcagtatga aatggccaaa gcgaaaggta
    18121 tccgtgtcga gcagatcatc cgtccgacag gattgatgga tccggagatc gaagtcaaac
    18181 cggccacgaa tcaggttgat gatcttctgg cggaaatcag agcgcggatt gaaaaaaatg
    18241 aaaaggtgct cgtgacgacg ctgacgaaac gtatggcgga aaatctgacg gattactacg
    18301 ccggtctgga tatcaaggtg cgctatcttc actccgatgt acacacgttg gagcgggtgg
    18361 acattatccg cgatcttcgt cggggcgttt ttgacgtcct gatcggcgtc aacctgctgc
    18421 gggaaggatt ggacattccc gaagtgtcgt tggtggccat tcttgatgca gacaaagagg
    18481 gattcctgcg atcggaacgt tcactcatcc agaccagcgg ccgcgccgcg cgcaatgttt
    18541 ccgggaaagt catcatgtat gctgataaga tgacccactc gattcaagcc tgtttggatg
    18601 agacaaaacg gcggcgcgcc attcagcaga aatacaatga agaaaataac atcacccctg
    18661 agaccattaa aaaatcgatc agcagtgttc tgggatcgat ttacgaagcc gattatgtaa
    18721 cgttacctgc aaccgtgtcc gaaagagtcg ctccgctaaa ggaagaagac ctcccggctt
    18781 tcattgcaaa actgaccaga gaaatgaagc aggccgcaaa aaatatggaa ttcgagcacg
    18841 ccgccgaact gcgggacgaa atcagagagc tgaacaaaat attgctggaa atgggttagt
    18901 tctactttcc ttcaatatat ggtataaata tttattgccg atgtatgttg agaacattcg
    18961 attctgggaa ttacggcagg atgaatgaat cggtcacaaa aaaggggtga agcatgaaag
    19021 cactaaaatc gatattatgc gtgttgacgt tatttgtttt tgtatttgcc ggttgcgaaa
    19081 aagaggcgtc cgattgggtt cagtaccata aagatgccga cgggaatgtc tattggtatg
    19141 atcagaacag ggtaaaagtg gacgcggcaa attatacggt tgaggtctgg gcaaaacaag
    19201 tctattccga tatgggcaga acgatagaac tgcagtccag aatcaaggat ggactgtcca
    19261 tgaaagggta tgacaagctt gcgcataaaa catgccggta tgcgatcgat tgcgggaaaa
    19321 aaggtataac ggtattgtcc atcagccatt ttgacagcga cggagagtcg ttatatttcg
    19381 atgtcgataa agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntccatcag
    19501 ccattttgac agcgacggag agtcgttata tttcgatgtc gataaaggtg aaaagaaaat
    19561 gtttattatc aaccctggct ctccagcggc ggcccttcaa aaagaagttt gctcgaagaa
    19621 acagtgatcc tgttgcgagc cgggggcaga aatgatcatt ggtaattgtc cgggcgcaga
    19681 gattgaaatg aatgtctcat gaaattattg aagaggaatg cttgtgaata aagtagtggc
    19741 acgattggca aatggaaaaa tgatcaaggg aacaaccgca gatttttttc cgggaaaaga
    19801 cgtttttcac gtaaccgaat cggcggccgc gccgggaacc aaggcgttgg aagtgttcac
    19861 gaaggatatg aaagcgttat tctttgtgaa agatctggca ggcaatccgc agcacgtcaa
    19921 aaccaacgag tttaatgcat cgcaaccggt tgtcgggcgc aagatcaaag tggttttcaa
    19981 ggacggggaa gttctgatcg gcaccacgac aggctatcaa cccggacgtc cggggttctt
    20041 ccttgtgccg gcggatgccg gatccaataa tgaacgttgt tatgtcgtga cggcgtcggc
    20101 caaggaaatt agttttctgt aagataagcc gctgaagggc cgggccgttt gaggagggtc
    20161 tcaaagggcc aaaattattc aatggaaagg taaaaatcag atgccgcaga aacagaaaaa
    20221 gaagaaaaga gatatcgaaa aagattatac cgccgggcag tttgtcatga aactgagacg
    20281 gctggctgac tgtatcgaaa ccggtcatcc attccgtata cagattgcag gagaaaaaat
    20341 tacaattcca accgatgccg tcatcaacat cgaacatgaa cgtggcacct cagaagagga
    20401 agtggaattt cagattaaat ggaagctgaa ccggtaagca tcaaaaatat ctttcccatc
    20461 atgatcttga taaaaaaacg gcttcgatga aaatgattta atgggatgtt ttgaaagacg
    20521 ggagacacac accatcatgc tggacgatat ctatcatgga tacctggact ggaacatgat
    20581 gtttgctgcg gaaagactcg gccgtcttga tgcacaaaaa acagggattg cggcttatct
    20641 tgcccgggac agttattaca acgatccgcg ttatgcggaa acgctgctga cacttggttt
    20701 cttcgatgtc cagttatttt accacaacca tttacaggcg ctgatcggat gccgcggtat
    20761 tcagaccccg gaaggagcca aagcgctggt tgtcattgtg ttcagagggt cggaccaggt
    20821 ccaggactgg ctccagaatc tggatattcg aatgacgcgt ttcctgaata aagaaaaatt
    20881 tcctcatttc agccgcctga aggttcacaa tggaattttt gaaagggttc acgattttga
    20941 agactggagc agacgcgtta ttttcaagga cggtccgttg aaaggcaacc tgcttcagat
    21001 gctgggagat gaacttaaaa gaaagcaatg tttcttctgg attatcggac atagcctggg
    21061 cggagccatg gcaacgcttt atgccgcgcg attatgcgac tgttacaatg tttcccggaa
    21121 cagaattctt tcccatacat ttggcgctcc gcccattggc aaccggtatt ttgcggcacg
    21181 ctatggacat gaatcttgtc ttcgggccca aaaaatcaaa aggaagagcg attctctcgc
    21241 gttattccgt tttgtcaata ccgaagatcc cataccggca ccgagatttg ccgatgtcag
    21301 agttgagaag gctcaaggcg gaccgattgc acaaccgccg tatcgattgc tggggtttaa
    21361 acatgtcggc aaggaagtga gattttcacc gcgccacctg ccgggctttt atgaaaaata
    21421 taagcagttc acgggaaggc ggtataacag ctcccacttt atgaaagttc attttatgga
    21481 agcttacatc accggaatcg atgtgttgaa tacgcattga gtcaattgcg catgacggaa
    21541 aatgtgccgg agtgagttga agagtgtttt accggttgaa gaggcggtca ttattcagaa
    21601 atcaattgac acgtaaaagt aacttcctta tattcaaaga agacgttaat caatgatcaa
    21661 cgggacacag gcgaatcatc gtaagcggtt taaagtaaaa tgaccatcaa ccattcaaca
    21721 aggaggacat tatgccgttt gaaatgatga aaaaagttat gttgacagga atcg
//
LOCUS       DAOSME010000039        21406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3050_length_21406_cov_21.004075, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000039 DAOSME010000000
VERSION     DAOSME010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21406
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3050_length_21406_cov_21.004075"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            524..1429
                     /locus_tag="PLG98_07115"
     CDS             524..1429
                     /locus_tag="PLG98_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOE33062.1"
                     /translation="MSNEQKNIMLNPLSQNTGNDNPDAILIQHALDGNRKSLEQLVAD
                     HQPWIYNIAFKMIMDHDDAADITQEILIKIITNLASYNPDRGNFRTWAYRIVVNHVLN
                     MKKRKFETRIYDFDRYIAAIENFPDDSSFTHPDAGLLAEEVKTGCMMGMLLCLKRTER
                     IAFLLGAVFAVSDAVGSELMDISRENFRQLLSRSRRKVFNYMNGTCGHMNPANKCRCS
                     HKVKSFLDLGMLDSAILRYHRPNSRMVKDMLGSRLERFHLSYYEPFLALFREQPFYDP
                     PDVTSWLDDILRQEDLKAIFNLGEE"
     gene            1487..2122
                     /locus_tag="PLG98_07120"
     CDS             1487..2122
                     /locus_tag="PLG98_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_272502274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1712..1714,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HOE33063.1"
                     /translation="MNHFKKYFLTFLLVTCLPIIGWASDQQEITAAIDYPQLEKRIAD
                     LSVTNMKGEEVAFESLWKHNRVVLVFIRHFGUMFARQRAAALMEIKKQIEANGAKILI
                     IGSGTPEQAKTFAENFNVELDIYVDESLQTYNAFSLERGFFKTLGWKSLKCGVRAMQS
                     GFHQGLSAGDLWQQGGVFVLGPGKQIVYQHVEQYAGEPLEKDEVLQAIKKR"
     gene            2404..3483
                     /gene="mutY"
                     /locus_tag="PLG98_07125"
     CDS             2404..3483
                     /gene="mutY"
                     /locus_tag="PLG98_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOE33064.1"
                     /translation="MMKDTDKIKKLLNRRLLAWYNRNQRLLPWRKTNDPYRIWISEIM
                     LQQTQVDTVIPYYYRFLETFPSVSSLARAPLQDVLKVWENLGYYSRARNLHAAAKVIV
                     EKFGGQIPDKLEEIKTLPGIGQYSSGAILSIAYKQAFPAVDGNVRRILCRVFAIRKPV
                     DDAREQKQLQELAASLIPVNHPGDFNQALMDLGATICKAKNPDCSRCPVANLCQARIK
                     SLQNVLPIIKKTPAIPHRQAAAAVIRNAKGRLLLVQRPASGLLASLWKLPGGFVETGE
                     DIERSLRRSVKEELGISIQTEKHLASVNHTYTHFRITLHAYNCRLLKSSPKPIGCQNS
                     RFVSPVDLKKLPMSKIDRLILDEIL"
     gene            3795..4544
                     /locus_tag="PLG98_07130"
     CDS             3795..4544
                     /locus_tag="PLG98_07130"
                     /inference="COORDINATES: protein motif:HMM:NF017062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF707 domain-containing protein"
                     /protein_id="HOE33065.1"
                     /translation="MMKNKNLVIAAVGDSSLHKQWKSGNPAFDLILIYYGNSDLIFFD
                     YTKDALICIKQHGQKFPLIKSFIETNLELVSQYDYVWLPDDDISISAGDLDRLFDTAR
                     TYSLQICQSALVSLHGDVSHAITKPERENTLRFTNFVEVMMPLFDVNTLLFLRNDFDL
                     SQSGWGLDATWSHRLNSPKDKIAIIDDITAVHTKPVGSDYSRFKIKPETELKHLLEKY
                     NIAFIIKSYSSILKNGGHKIPWYRKFFRFSS"
     gene            complement(4686..>4968)
                     /locus_tag="PLG98_07135"
     CDS             complement(4686..>4968)
                     /locus_tag="PLG98_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33066.1"
                     /translation="EDVIGDDVWGQTFTKQPKSLTRVQKQKWLSKVTGVCLGSDAFFP
                     FGDNIERARRSGVTAIVEPGGSIRDQQVIDTCNKYRIVMAFCGLRLFHH"
     assembly_gap    4969..5068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5069..6019)
                     /locus_tag="PLG98_07140"
     CDS             complement(<5069..6019)
                     /locus_tag="PLG98_07140"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF005492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOE33067.1"
                     /translation="MNEIQLKYGCNPNQKPARIFMADGSHLPVDVLNGKPGYINFLDA
                     FNSWQLVKELKENTGMVAAASFKHVSPAGAALGYPLDRVLRKMYHIDSKIKLSPLACA
                     YARARGADRMSSFGDWIALSDACDVSTAKIIKNEVTDGIIAPGYEPEALEILKSKKSG
                     NYNVVKINPDYVPVSLEHKQVYGITFEQGRNDLKIDSRMLENIVTENKTLTDAQKRDL
                     VLALITLKYTQSNSVCYVQDGQVIGVGAGQQSRIHCTRLAGQKADNWQLRHMPKVLNL
                     PFRDDVAKPNRDNAIDVYLGETPEDVIGDDVWGQTFTKQPK"
     gene            complement(6022..6750)
                     /locus_tag="PLG98_07145"
     CDS             complement(6022..6750)
                     /locus_tag="PLG98_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOE33068.1"
                     /translation="MKNNLYEFLAGNDYPGRGICIGMTPGGKAVIAYFIMGRSTNSRN
                     RVFDPIEGGIRTKAADPSKMTDPHLIIYNPVLIFAKTTIVTNGDQTNTIYDFMSQNKF
                     PGFHFEAALNTRTFEDDSPNWTPRISGMVDMKTGAYKINILKSCGGDEKSVQRFTFNY
                     AQPLSGEGHFISTYKCNGTPIPSFQGEPMRVIIDEEDPDRYAAKLWDALNEENKVSLF
                     VRAVDLKTQTCKDVIINKYKAVEE"
     gene            6946..7455
                     /gene="purE"
                     /locus_tag="PLG98_07150"
     CDS             6946..7455
                     /gene="purE"
                     /locus_tag="PLG98_07150"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOE33069.1"
                     /translation="MVKRNALVSVVMGSDSDLPVMTEAAKVLEEFGIAHELILTSAHR
                     TPLRTTQFASAAAGRGIKVIIVGAGAAAHLAGVIASQTPLPVIGVPIDATALNGLDAL
                     LSTVQMPGGIPVATMAIGKAGAKNAALFAVRMLALDNKTLGKKLRDYIRKMAKDVEKK
                     QENLTCRKS"
     gene            7440..8078
                     /locus_tag="PLG98_07155"
     CDS             7440..8078
                     /locus_tag="PLG98_07155"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOE33070.1"
                     /translation="MPEILKPGTDISEDAMISRAVKILTDGGIIAYPTETFYGLGVDA
                     THEEAIGKIFAVKGRNFKNPVSLIIAKPEDINVLVTDVPETAKKLIDAFWPGALTIVF
                     QASDTLSPLLTAGTGKIGLRVSSDPIALKIVQNIKKPLTATSANLSGAPECSTASEVA
                     GQIGGKIDAILDGGKTPGGASSTLIDVTLNPPVVLREGSISRQSIKEHLHVI"
     gene            complement(8225..9562)
                     /locus_tag="PLG98_07160"
     CDS             complement(8225..9562)
                     /locus_tag="PLG98_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33071.1"
                     /translation="MMANVMDKYFNMRIILCVALMSMVLLLQACSINQVYLSDSDEED
                     YSAEDITDQEMMRVAKVKLQSTGMVKRAFDIEERHRKLTLRETRQLLKILSQRFKKSG
                     LSVGEFEDLVLERIGRDLEIISAFNERLKNAEAKEKDHGYELCKKNELLEVIKGSEDI
                     VEEYIEDMVNGILPEEFETSLSEITRLERTKEDDIVPGSFKPTCFKGALITMLVALRG
                     DRANVSALKLSGGTWSNSSIIFHTIPMPGKISRSSIHPDFESFTYYYKGASSPSQGEL
                     GFPHSGYYAIGYGARKSSKGIANPEDCSSWIFHQVLQTQYPVTSYDVMQFFRIKTGTG
                     IRHTGYAATPEYGWLNSVFIPVEKITKKNARAMIKPGYVLCIRRPDEDGKVIRNQNYG
                     VSGHMAIALFATDTHIFAVACTRDVPLMEGIGIQKISFWKERNSDIMILRMNK"
     gene            complement(9739..10617)
                     /locus_tag="PLG98_07165"
     CDS             complement(9739..10617)
                     /locus_tag="PLG98_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33072.1"
                     /translation="MRYEGNIFRPFSEANSYLLQCTVGCSHNQCTFCGMYKDKKYRER
                     SLDEIMEDIAMAKDHYGDLEKVFLCDGDAIALKTDMLLTILKKLYGTFPSLRHVGSYV
                     GPQSTLSKSLEELQTLRAAGLTKAYIGVETGDDELLKKIKKGVGYEEMLQAGRNLIAA
                     GINLSAMVLLGLAGRGKASRRHALATAKICNEMKPQYLAALTVTPVPGTVLYRQVQRG
                     EFELLDAFETLEEMKQIFENITADNMKFVGTHASNYLPVVGTLQKDKQKMIAMVDEVL
                     KNRDERMLRPDHMRGL"
     gene            complement(10688..11722)
                     /locus_tag="PLG98_07170"
     CDS             complement(10688..11722)
                     /locus_tag="PLG98_07170"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HOE33073.1"
                     /translation="MIIQCRQCRTKFRFDDSHMQGSGIWLRCSRCGHVYFQENPFAKR
                     TDSGALPLTEPDLETKPVNEEEKTAPSETVSVPPEEAPSIIRRDERGLKSIDKIMTAR
                     KRDREGIDLDIATFRDEKVGEADGQTGDIVQEEQEVNVEETDHQKKTLPKGSGRIWKT
                     ALWSVLVIGVIPLLIYFFILPQYGAQLVGSGEYLFKKISELRGAPELPTSGAYINQMV
                     KLQNVRYRRINNYILGQIGVLEGTALNRADFPLSRVQVKGEIVDAYSVVLGERSSYAG
                     NILTDEELINLSEEEILMKLLRPEGLNNANDRIMPNGQIPFMIVFPSEPSGVIKTTVM
                     TIGAERLLEP"
     gene            complement(11804..12337)
                     /gene="hpt"
                     /locus_tag="PLG98_07175"
     CDS             complement(11804..12337)
                     /gene="hpt"
                     /locus_tag="PLG98_07175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOE33074.1"
                     /translation="MDQASRKILFPKETIQKRVREMANQISKDYAGKELILIGVLKGA
                     FIFMADLIREINIPCRVDFARLASYGAGSESSGKVVLTKDIETSIKDKDILIVEDILD
                     TGLTMQYFVEWLKERNPKSLKTCVFLDKRKRRKVPLEADYVGFTIDDGFVVGYGLDFN
                     EMYRFSPDIYTIHSNKL"
     gene            complement(12527..13345)
                     /locus_tag="PLG98_07180"
     CDS             complement(12527..13345)
                     /locus_tag="PLG98_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33075.1"
                     /translation="MSNSNIFVLCQPDKGKSCGACCGLYNYVDSSRSSLARRLRSRTK
                     RFRETVKSPADLKRYATETLVAEDFKKRYDVIHCCEYLGFLDHDEKKVGCLVHPEQNA
                     GCDMRDVSFYGRELCAGHLCPSHYFIPQSQSQILIKIIDDWYLYGLVLTDIDLVVQYF
                     RLIADRVGAELKPDVFDHDELKEIALDYFGWKLNWPFRSQEANRLGKYYFDGSQYMIS
                     HIEYEKFGREVSVLNSILLSLCSSFKSAVQLEEAEKMIWANIEKFVSTYKRHVS"
     gene            complement(13516..14373)
                     /gene="purU"
                     /locus_tag="PLG98_07185"
     CDS             complement(13516..14373)
                     /gene="purU"
                     /locus_tag="PLG98_07185"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOE33076.1"
                     /translation="MNNVNAVLLLSCADSKGLVARISDFIFRFDGNIVHADQYTAKAE
                     KLFFMRVEWELNGFALDRDDIPVAFAPLAKQYQMKWDLHFTDYVPRAAIFVSRHLHCL
                     HDLILRRDMGELAADIKAVISNHEDARDLAEQYGMQYFYFPVTGKNKKQQEKKELRLL
                     RDLHIDLVVLARYMQILSGNFLKYYPSRIINIHHSFLPAFAGRNPYQQAYDRGVKIIG
                     ATGHYVTAQLDEGPIIAQDVVKISHRDTVTDIQRKSKDLERIVLARALRLHLENRILV
                     YGSKTIVFE"
     gene            14486..14884
                     /locus_tag="PLG98_07190"
     CDS             14486..14884
                     /locus_tag="PLG98_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33077.1"
                     /translation="MKLIEEIKLDNGLDLRIFDLSRSIATDTVKVEVSFQTNVLLKES
                     FFTSTEDYRLVKNIMGDELAYEHTMERTFVSKDNEDSTRNELISTFKHNSLGYLSAAN
                     FAQKMALSKLREIKSNPHKYRSHAQSDKKA"
     gene            14920..17397
                     /locus_tag="PLG98_07195"
     CDS             14920..17397
                     /locus_tag="PLG98_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOE33078.1"
                     /translation="MRHGKIDELLSSAEKPSRYIGTEVNAVRKDKADVRFLLAFPDTY
                     EVGMSHLGLQILYSIINDIPYASAERCFAPWPDREKILRDKKLPLTSLERQIPLQNFD
                     MIGFSLQYELSYTNMLNMLDLGGIPIDRKERRDGHPLIIAGGPCCFNPAPLADFIDAF
                     VIGEGEEIVGDIVAAVREGKIKSISRNGLLGELANIPGMYVPAVHHDNEIIKKRTVSD
                     LNAWRHPLKPVVPLMQTIHDRITLEIARGCTRGCRFCQAGMIWRPYRERNAALLMEMA
                     EKMLAATGHEEISLLSLSSGDYSCLEPLVKGLMNRYCQKRVALALPSLRVESLNTTLM
                     EEIRRTRKTSFTLAPEAGTDKMRLVINKGNTSEDLLSTADKIFAAGWKSIKLYFMLGL
                     PHETQEDWEGIVNLSHETLRAAKNRGQVTISLSTFVPKPHTPFQWEKQISLAETYERQ
                     DFIRTRIKNRNLSVKWHDARMSFLEGIFSRGNEQAGKLLGNAFRKGCRFDGWSDILRF
                     NLWEEAIAESDIHPDDYLRERGTDEKLPWGNIDCGVSREFLLEEKQKAFQQTATADCR
                     FETCRNCGACDFSITKNILAADNENKSVLPSSLAPETSSAQEEKYRVMFNKMESAGFL
                     SHLELATALVRALRRSSINLAYTGGFHPHPKISFATATAVGMESRQEYMDITAQEYSC
                     SLDLLRTEINTLLPTGIKILEIRKLSPEENAIAQATRGFEYELQLPAATDAPRMSAIK
                     ANIDQFLAASNFNIKKISKGKTITKDIRPFVQMLSLDRDKKIIKFIVSFVQEGSARPV
                     DIIAHILKSDPDESRQIRVVKTKTLLG"
     gene            17419..18120
                     /locus_tag="PLG98_07200"
     CDS             17419..18120
                     /locus_tag="PLG98_07200"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOE33079.1"
                     /translation="MRIWDVNPGYLNRQSLLGEHRELHAIVSIIQHNKKGYSRHPETL
                     RWHGFGWALSQRHRLLAAEMNLRGFTDRSPAMIKTQPQKWPHVFVDSPTSQFSILKKK
                     YVNKEQGRIPLPNSVQQLWAQHQYSVMARDEAEYKYIGGWVTSRKSGKGIHEIYPELV
                     SLLRCPPVEANLRNALEQMWNFVCAYAPHPATAMKKESSRHLLKQIQRTAVLHDIVYL
                     KESTALGELPAWQIG"
     gene            complement(18139..18825)
                     /locus_tag="PLG98_07205"
     CDS             complement(18139..18825)
                     /locus_tag="PLG98_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOE33080.1"
                     /translation="MRQFNSDRRLIVKTAQKLVEKGYLAATGGNISVRIKKGNAFAIT
                     PSNRNYMTMTAADICILDEHLNVLSGPWKPSMEAALHGAVYRTRGDVNAVIHTHQVYA
                     GALTIIKTPLPALFDEQVRFLGKTVAVIPYAPSGSVPLKNNVLRHIKNGNNAFLIQNH
                     GALVLGHDMDRAVHNVEILERYSLAYLLAICSGYRVSKIPMRMRETFFKMLRQEQKKL
                     QKDHCGSRKK"
     gene            complement(18939..19808)
                     /locus_tag="PLG98_07210"
     CDS             complement(18939..19808)
                     /locus_tag="PLG98_07210"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOE33081.1"
                     /translation="MKYGIIANPVSGKRSVDDKIKILKEVAGILKDATIAGLDTKSPE
                     EFSTAAHDLSQKVARLLVAGGDGTFSLVMNSVSRDIPLGFLPFGSGDVLRHTFNLPRC
                     PKENALLIQKGKEYLLDVICCDGKTKALFASIGIEGSILLERDNYLENNLTGFTAYGL
                     STLKILFDGYIKADLRVLMDKKEIRTFNSLTAVITKLPYYGYGFKVVPHAQFDDGYLH
                     LLILNAGPIGTLYALATSLAGGNRMGEYYKAKEIDITTSTEQLLQRNGDPEKEKRNNF
                     SFRILPKELKLIF"
     gene            complement(19946..20773)
                     /locus_tag="PLG98_07215"
     CDS             complement(19946..20773)
                     /locus_tag="PLG98_07215"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE33082.1"
                     /translation="MNAGAPVALISGCSTGIGRFLTTELAKRGWQVFATARNHAAIED
                     LKAPNVQTAALDVTDEKSIKTCVESVMANAGKIDMLINNAGLLLIGPLSELEISELRR
                     EFETNVIGLAALTHAVVPHMIKRTSGKIVNISSISGVLNTPFSGAYCSTKAAVTSYSD
                     VLRMELKPFGIDVITVQPGGIRSNLSDNADKGLERFRKTPYAPITDFIVDRAHMSQQG
                     ATPTDVFARQLVDKLVKSKTPRIIRIGKGATMYPFVAKLPRTFTDRLLSRKFGLDKL"
     gene            21112..>21406
                     /gene="rplU"
                     /locus_tag="PLG98_07220"
     CDS             21112..>21406
                     /gene="rplU"
                     /locus_tag="PLG98_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOE33083.1"
                     /translation="MYAVIKTGAKQHKVSEGDILSVEKLDGNKGDEVVFSDVLMVSDD
                     KEIKVGKPFVDGAKVVGEIIAQKKGPKLTVYHMKRRKGFHKKTGHRQNLTSMKI"
BASE COUNT         5574 a         5251 c         4883 g         5598 t
ORIGIN      
        1 aaatagtttt aaattgattg tccccttgct ggtttcaata accgctttta aatcacttgc
       61 cattttaatc tcctgttaag atgtgaagtg tcccctcccc cttgcaacgc ttcggggata
      121 attcgactga tgctttaata aaccgttatt tttcgaaacg tagcgccgga aaatcccaga
      181 gcgaattgtc ccgccacctt cgggtggctg ggacaaacat cattacgttc gttctcaacg
      241 agtttcgtta aactttaaag attggccatg aataagtcaa gacaaaaagg ataatgtgcc
      301 gtctttaccc gcatttacgc ccccggctct tcaggttccg ccttgcactt ttaaactcat
      361 gtttgtcttg ctttttattt catttcatat tctcctgccg ggaaaagaat acaacgcagc
      421 ctctggagtt aaaatagtga aataataata ttattttagt agataatcac ctgaacatga
      481 ttttttctgt cacagaggtg aattgcaatt gtataagggg agtatgtcaa acgaacagaa
      541 aaacataatg ctcaacccct tgagccaaaa taccgggaat gacaatccgg atgctattct
      601 tattcagcat gctctggacg gcaacagaaa atccctggag caactggtgg ccgaccatca
      661 gccatggatt tataacatcg cgttcaagat gatcatggat catgacgacg cggcggacat
      721 cacccaggaa atactcatta aaatcatcac gaatctggca tcctacaacc ccgacagagg
      781 aaatttcagg acgtgggcct accggattgt ggtcaatcat gtgcttaata tgaaaaaaag
      841 gaagtttgaa acgcggatct atgacttcga ccgctacatt gccgccattg aaaattttcc
      901 ggatgacagc tcatttacgc atcccgacgc gggccttctg gccgaagaag tcaaaaccgg
      961 ctgcatgatg ggtatgctgc tgtgcctgaa gcgcaccgag agaattgctt ttcttctggg
     1021 agccgttttt gccgtgtccg atgccgtcgg aagcgagttg atggatatat cgcgggaaaa
     1081 tttccggcaa ctgctttcgc gcagccggcg caaggtattt aattacatga acggaacctg
     1141 cggccatatg aaccccgcca acaagtgtcg ctgctcgcac aaagtgaagt cctttctcga
     1201 cctgggcatg ctggattccg caatactcag gtatcaccgc ccgaactcgc gcatggtgaa
     1261 ggacatgctg ggctcccggc tcgaaaggtt tcacctgtcc tactatgaac ccttcctggc
     1321 gttattcagg gagcagcctt tttatgatcc gccggatgtt acatcctggc tggacgacat
     1381 cctgaggcag gaggatctca aagcgatttt caacctcggg gaagaatgat gcaaacccgc
     1441 aatcgttgga aaaaattaaa tacgggtaag aaaggagacg tgttgcatga atcactttaa
     1501 gaaatacttt ttaacatttt tgctggtgac gtgtttaccg atcatcggct gggcaagcga
     1561 tcagcaggaa ataactgctg ctatcgatta tccccagttg gaaaaacgca ttgcggatct
     1621 ctccgtcacg aacatgaagg gcgaggaggt tgcttttgaa tccctttgga aacacaatcg
     1681 cgttgtgctg gtgtttatac gccatttcgg gtgaatgttc gccaggcagc gggctgctgc
     1741 cttaatggag ataaaaaaac aaatcgaggc aaacggagca aaaatcttga ttatcgggag
     1801 cggcacaccc gagcaggcaa agacatttgc cgagaatttc aacgtcgaac ttgatatcta
     1861 tgtcgatgaa tctcttcaga catacaatgc cttttcgctt gagcgcggat tcttcaagac
     1921 cctggggtgg aaatcactca agtgcggtgt tagagccatg cagagcggct tccaccaggg
     1981 cttgagcgcc ggtgacctct ggcaacaagg cggggtattt gtcctgggtc cgggcaaaca
     2041 aatcgtttat cagcatgtgg aacaatacgc gggcgagccg ctcgaaaaag acgaggtttt
     2101 gcaggcgatt aaaaaaagat aagccactgg ttcacccgca gccggaacat gcattggcgg
     2161 cataaccaaa acaccacagg cgggagaagg tgtcgctcca ggattaaacc ggggcggcgc
     2221 cttttctcct cttcaaacat tatattaaaa agtcaacaca ctgcctttcg gaaaagaaat
     2281 cttaacgcat caggattcct cgcctaaaat attgtcctga aattgcctga tgatcaacgc
     2341 agacatgaaa tatcgccaat taagatttga ttcctttcgc tggtatagga tatacagacc
     2401 acgatgatga aggataccga caaaatcaaa aagcttctga accgccgact gctggcctgg
     2461 tacaaccgca atcaacgctt gcttccctgg cgcaaaacaa atgatcctta ccgtatctgg
     2521 atttcggaaa ttatgcttca gcaaacccag gtggatacgg taattccata ctattatcgt
     2581 tttctggaaa cattcccgtc cgtttcctca ctggctcgcg cccctttgca ggatgtgctg
     2641 aaagtctggg aaaatctggg ttattattca cgggcccgaa atcttcacgc tgccgccaag
     2701 gttattgtgg aaaaattcgg tgggcaaatc cctgacaaat tggaagaaat aaaaactctc
     2761 cccggaatcg gccaatactc atccggtgcc atcttaagca tcgcttataa acaggcgttt
     2821 cctgccgtgg atggaaatgt ccgccggatt ttgtgccgtg tatttgccat ccgcaaaccg
     2881 gtggacgacg cacgggaaca aaaacaattg caggagctcg ccgcgtcgct gattcccgtc
     2941 aaccatccgg gtgatttcaa ccaggccctg atggatctgg gagcgacaat ctgtaaagcc
     3001 aaaaatcccg actgttcccg ttgtcccgtg gccaaccttt gtcaggctcg aatcaaaagt
     3061 ttacaaaacg ttttgccgat aatcaaaaag actcccgcaa tcccgcatcg acaggcagca
     3121 gcagccgtca tccgcaatgc aaaaggcagg ttactgctcg ttcaacgccc tgcatcagga
     3181 ttattggcat ctttgtggaa actgcccggc ggtttcgttg aaacgggtga agatattgaa
     3241 agaagtttaa ggcgaagcgt taaagaagaa cttggcattt ccatccagac ggaaaaacat
     3301 ctggcctcgg tcaatcatac gtatacgcac ttccgcataa cccttcacgc ctacaattgc
     3361 cgtttgttga aaagctctcc caaacctatc gggtgccaga actcgcgatt tgtatcacct
     3421 gtcgatttaa aaaagttgcc catgtcgaag atcgacagat tgattctgga tgaaatctta
     3481 tgacgttatt tttgcatatt gagaaaatca attcgattaa caaaggacat gtgccttttc
     3541 tttaccatac ttcacctctt caaaactcaa tcgtgcacag aagaaaatca tcacgagtgc
     3601 tttacacctc aacagaagcc ttgacacaca gggcaccgtt tcatatattc gccccgcgag
     3661 aagaacgcgc ttatccatat tgataagatg ttgagagcgg ggaaatatta aatgccatat
     3721 gccgtatcaa aagatcaccc gagctgttaa cggtaaacgt ccattattct gttcaatatt
     3781 cttggcccga ttgaatgatg aagaacaaaa atttggttat agccgctgtc ggagacagct
     3841 ccctgcacaa acaatggaaa agcggcaacc ccgcctttga tttgatcctc atttattacg
     3901 gtaacagtga tttgattttt ttcgactata caaaagatgc cttgatatgc atcaaacaac
     3961 atggtcagaa atttccttta atcaaatcct ttattgaaac gaatttggaa ctcgtatctc
     4021 aatatgacta tgtttggctt cccgatgatg acatttccat atcagcgggt gatctggacc
     4081 gtctttttga tacagcccgg acatactctc tacagatttg ccagtccgcg ttagtatcct
     4141 tacacggcga tgtatcgcat gccataacga aaccggaaag agaaaacacg ttaagattta
     4201 caaactttgt cgaagtcatg atgccgctgt tcgatgttaa cactctcttg tttctccgta
     4261 atgatttcga tctttcccaa tccggctggg gcctggatgc aacatggtct caccggttga
     4321 attccccgaa agataaaata gccattattg acgatatcac cgccgtacac accaaacccg
     4381 tcggcagcga ttattcaaga ttcaaaataa agccggagac agaactgaag catctgctgg
     4441 aaaaatacaa tatcgctttt ataataaaaa gttattcatc catattgaaa aatggagggc
     4501 ataaaatccc gtggtacaga aaatttttca ggttttcatc ttagaataat aatgagtatg
     4561 taaacgtttt ttgacttccg atctttctgt ttgctgtctg cattcgggcg agtcttttgg
     4621 actcgcccgt cttgattacg gtcaattttt tttattcctg ttcctgcgcc agcatgacag
     4681 aaatatcaat ggtggaacag gcgcaggccg caaaacgcca tcacgatcct gtatttgttg
     4741 caggtgtcga tgacctgctg gtcgcggatc gacccccccg gttcgacaat ggcggttacg
     4801 ccgctgcggc gggcgcgttc gatattatcg ccaaacggga agaacgcgtc gcttcccagg
     4861 caaacgccgg ttaccttgct gagccatttc tgcttttgca ccctggtcag ggatttgggc
     4921 tgcttcgtaa atgtttggcc ccagacatca tcgccgatga catcttccnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tgggctgctt cgtaaatgtt tggccccaga
     5101 catcatcgcc gatgacatct tccggtgttt cgcccagata aacatcaatg gcgttgtcgc
     5161 ggttgggttt ggcaacatcg tcgcggaaag gcagattcaa cactttcggc atatggcgca
     5221 gttgccagtt gtcggctttc tgtccggcaa gccgcgtgca gtgaatgcgg ctctgctgtc
     5281 ctgcgccgac accgataacc tggccgtcct gtacatagca aacgctgttg gactgtgtgt
     5341 atttcaaggt aatcaacgca agtacgagat cgcgtttctg cgcgtcggtc agtgttttgt
     5401 tttcggttac aatgttttcc agcatgcggc tgtcgatttt caagtcgttt cgtccctgct
     5461 cgaacgtgat gccgtaaacc tgcttgtgtt ccagggaaac gggcacgtaa tcgggattga
     5521 tcttcaccac gttgtaattg ccgctttttt tggatttcaa gatttccaga gcttccggct
     5581 cataacccgg cgcgatgatt ccgtcagtaa cttcgttttt gattattttg gccgtggaca
     5641 cgtcgcacgc atcggaaagc gcaatccagt cgccgaaact gctcatgcgg tcggcgccgc
     5701 gggcgcgcgc gtaggcacag gccagcgggg agagctttat tttggaatcg atgtgataca
     5761 ttttgcgcaa cactctgtcc aaaggataac ccagagcagc gcctgcggga gatacatgtt
     5821 taaaggaggc tgccgccacc atgccggtat tttccttgag ttctttgacc aactgccagc
     5881 tgttgaaggc gtccaaaaag ttgatatagc cgggcttgcc gtttaagacg tccacgggca
     5941 gatggctgcc gtccgccatg aagattctcg ccggtttctg attgggattg cagccgtatt
     6001 tcagttgtat ttcgttcatg atcattcctc cactgcttta tatttgttaa taatgacgtc
     6061 tttacaggtt tgcgtcttga gatcgacggc gcgcacaaaa agactgactt tgttttcttc
     6121 atttaatgca tcccagagtt ttgcagcata ccggtcggga tcttcttcat cgattatgac
     6181 gcgcatcggt tcgccctgaa agcttggaat cggcgtgccg ttgcatttat aagtgctgat
     6241 aaagtgtccc tcgccggaca gcggctgagc ataattgaag gtaaaacgct gcacgctttt
     6301 ctcatccccg ccgcaacttt ttaaaatatt gatcttgtaa gcgcctgttt tcatatccac
     6361 cataccggaa atacgcggcg tccagttggg actgtcgtct tcaaacgtgc gggtatttaa
     6421 cgcggcttcg aaatgaaagc ccggaaattt gttttgcgac atgaaatcat agattgtatt
     6481 ggtctgatca ccgttggtca cgatggttgt tttggcaaag atcagtaccg gattgtaaat
     6541 aatcagatgc ggatcggtca ttttggacgg gtcggcagcc ttggtgcgga tgcccccttc
     6601 gatcggatca aatacacggt tacggctgtt ggtgcttctg cccatgatga agtaggcaat
     6661 cacggctttg ccgccgggcg tcatcccgat acaaataccg cgtcccggat aatcgttgcc
     6721 ggccagaaat tcatataaat tatttttcat tttttacatc tctccttgag tgcgggtaag
     6781 aaattttggc gggagtatat gaagatcata ttgttatgtc aactggattt gggaaaaaga
     6841 tcgattttat aataactatt gcaaatgaag gcccgatgcg ttagatataa aaaataataa
     6901 aaagcaataa tatcagccaa taaaggagcc tgcatgaaag caaaaatcgt caagaggaat
     6961 gccttggtca gtgtggtaat gggcagcgat tcggatcttc ccgttatgac cgaagccgca
     7021 aaggtactgg aagaattcgg catcgctcat gaactgattt tgacgtcggc acatcgcacg
     7081 ccgcttcgca caacacaatt tgcttcagct gcagccgggc gcggcatcaa ggtgattatt
     7141 gtcggcgccg gagcggcggc tcatctagcc ggtgtgattg cctctcagac gcccctgcct
     7201 gtcatcggcg ttccgattga cgctaccgct ttaaacggac ttgatgcgct tctttccacc
     7261 gtgcagatgc ccggaggcat tcccgtggct actatggcta tcggaaaagc cggtgccaaa
     7321 aacgccgcac tgtttgccgt tcgcatgctt gcgcttgaca acaagacatt gggaaagaaa
     7381 ctccgcgatt acatccggaa aatggctaaa gacgtggaga aaaagcagga aaatctcaca
     7441 tgccggaaat cctgaagccg ggaacggaca tttccgaaga cgcaatgatc agtcgcgccg
     7501 taaaaatcct gacggacggc ggtatcattg cctatccgac ggaaacgttt tacggtctgg
     7561 gagttgatgc cacccatgaa gaagccatcg gtaaaatctt cgccgtcaaa ggccggaatt
     7621 ttaaaaatcc cgtctcttta atcatcgcaa aaccggaaga tatcaatgtg ctggtcacgg
     7681 atgtaccgga aacggcaaaa aaactgatcg acgcgttctg gccgggtgcg ctgaccattg
     7741 tttttcaggc gtcagacacc ctgtcgccgc tgctgacggc gggaaccggc aagatcggtc
     7801 tgcgtgtttc gtctgatcca atcgctctga aaatcgttca aaacataaaa aaacccctga
     7861 ccgccaccag cgccaattta tccggcgcgc cggaatgctc gacggcttca gaagttgccg
     7921 gacaaatcgg cggcaaaatt gacgcaattc tggacggggg gaaaacgccg ggcggggcat
     7981 catccactct gattgatgtt acgttgaatc cgccggtcgt ccttcgtgaa ggatccatca
     8041 gcaggcaatc gattaaagaa catcttcacg tcatttgatc agcaagtaaa agccccttca
     8101 catcgaagtt tcatcgtaac ggatatgatt ttttttgggt gagtcgtaac caaatcggcg
     8161 actggcatcg gccatcagag gggtgatgca gtcattgaat cggagcgtag cttcatcaaa
     8221 cgcgttattt attcattcgc aaaatcatga tatcgctgtt tctctctttc caaaagctga
     8281 tcttttgaat accgatccct tccatcagcg gaacgtcgcg tgtgcaggcc acggcaaaaa
     8341 tgtgggtatc tgtcgcaaat agcgcgatag ccatatgacc ggaaactccg taattctgat
     8401 ttcgaatgac tttaccgtct tcgtccggtc gccgtataca caatacataa ccgggtttta
     8461 tcatcgcccg cgcgttcttt ttcgttattt tctctacagg gatgaacacc gagttaagcc
     8521 agccgtattc aggagtggcg gcgtaacctg tgtggcgtat tcccgtcccg gttttaattc
     8581 tgaaaaactg catcacgtca tacgaggtca ccggatattg cgtttgcaat acttggtgaa
     8641 atatccagga agagcaatct tcaggattgg ctatgccctt ggaggatttt ctggcgccgt
     8701 agccgatggc ataataaccg ctgtgcggga accccagttc cccctgcgag gggctggaag
     8761 cacctttata ataataggta aatgattcaa aatccggatg aatcgatgac ctgctgattt
     8821 ttcccggcat cggaatagta tgaaagataa tgctggaatt tgaccacgtt ccgccggata
     8881 actttaatgc cgacacattg gcgcggtcgc ctctcaaagc gaccagcatg gtaatcaggg
     8941 ctcctttaaa gcatgtgggt ttgaatgatc cgggcacaat gtcgtcctct ttggtccgtt
     9001 caagtctggt gatttcagat agcgacgttt caaattcctc cggcaggata ccgttgacca
     9061 tgtcttcaat atattcttcc acaatgtctt cagaaccttt aatcacttcc agcaattcat
     9121 tttttttgca caattcatac ccgtgatctt tttctttcgc ctccgcgttt ttcagtcgct
     9181 cattaaaggc gctaatgatt tcaaggtcgc gacctatcct ctccagcacc agatcttcaa
     9241 attcgccgac ggacaagccg cttttcttaa atcgctggct caaaatcttg agcaattgtc
     9301 tggtttccct gagggtcagt tttctgtggc gctcttcaat atcaaaggct ctcttgacca
     9361 tacccgttga ctgtaatttc acttttgcaa cgcgcatcat ctcctgatcc gttatatctt
     9421 ccgcgctgta atcctcctcg tctgaatcgg acaaataaac ctgattgatt gaacaggctt
     9481 gcaacaataa caccatgctc atcagcgcga cgcataagat gattctcata ttgaaatatt
     9541 tatccatcac atttgccatc attgtccgcc gccatttaaa aatatttttt taccctgata
     9601 ttgccccgaa gcataaaaga caccctgctt tcgtgtcaag aaaatcttga acaccggata
     9661 ccatgatcat acatccatgt ccccgataaa cacaattttc gtctaaacga cctgctgctt
     9721 cattgcagaa aaaatcattt acagtccgcg catgtgatcc ggacgcaaca tccgctcatc
     9781 tctgtttttc aaaacttcat cgaccatggc gatcattttc tgtttgtctt tctgcagcgt
     9841 ccccacaacc ggaagataat tggaggcatg cgtgccgaca aatttcatat tgtcggcggt
     9901 gatattctcg aatatctgct tcatttcttc cagggtttca aaggcgtcca gcaattcaaa
     9961 ctcgccccgc tgcacctgac ggtacagcac cgtgcccgga acaggcgtca cggtaagcgc
    10021 ggccagatac tgcggtttca tttcattgca gatttttgcc gtggccagag catgccgtcg
    10081 agaggccttt cctcgcccgg ccaggcccag aagcaccatc gcggacagat tgattcccgc
    10141 cgcgatcagg ttccgaccag cctggagcat ttcttcataa ccgacgcctt ttttaatttt
    10201 tttcaaaagt tcgtcatcgc ctgtttccac accgatatat gccttggtca aaccggcagc
    10261 ccggagggtt tgcaattctt ccagggattt ggacagcgta ctttgcggtc cgacatagct
    10321 tccgacatgg cgcagcgacg gaaatgtccc gtacagcttt ttcagaatgg tcagcagcat
    10381 gtctgttttc agcgcaatcg cgtcgccgtc gcagagaaaa actttttcca gatcgccgta
    10441 atggtctttg gccatggcaa tgtcttccat gatttcgtca agcgaccgtt ccctgtactt
    10501 tttgtccttg tacataccgc agaaggtgca ttgattatgc gagcagccga ccgtgcactg
    10561 cagaagataa ctgttggctt cactgaacgg ccggaaaata tttccttcgt atctcaattt
    10621 tgactccttt caatcgggga cgaatgcgtg ctgatttttt gaatactgcg gtcaatgaat
    10681 atcccgatca gggctcaagg agacgttccg cgccgatggt catgaccgtt gtcttgatga
    10741 cacccgaggg ttcgctgggg aaaacaatca tgaaagggat ctgtccgttg ggcataatcc
    10801 tgtcgttggc gttattaaga ccttccggac gaagcagttt cattaaaatt tcttcttcag
    10861 acaaattaat cagttcttca tcggtcagga tattccccgc gtaactggaa cgttcaccca
    10921 aaacgaccga gtaggcatcc acgatttcac ctttcacctg aacccgggac agcgggaaat
    10981 ccgcacgatt cagcgccgtg ccttcgagaa caccgatctg gcctagaatg taattgttga
    11041 ttctgcggta acgcacattc tgaagcttca ccatctggtt gatataagcc cctgatgtcg
    11101 gcagttccgg agcgcctctt aattcgctga tctttttgaa gagatattca ccgctgccga
    11161 ccagctgtgc gccatattgc ggtaaaatga agaaatagat caacagcggt ataaccccta
    11221 tgaccagaac cgaccagaga gccgttttcc agattctgcc ggaacctttc gggagcgttt
    11281 tcttctgatg gtcggtctct tcaacattga cttcttgttc ctcttgtacg atgtcacccg
    11341 tctgtccgtc tgcttcgccg actttttcat cccggaaagt ggcgatgtcc agatcgatcc
    11401 cttcgcggtc acgctttctg gccgtcatga tcttatcgat ggatttcagg ccgcgctcgt
    11461 ctctgcggat gatggaaggc gcttcttctg gcggcactga caccgtttcg gaaggagctg
    11521 ttttttcttc ctcgttgacc ggtttcgttt caaggtccgg ttcggtcagc ggaagcgcac
    11581 cggaatcggt tcttttcgca aaaggatttt cctgaaaata cacatggccg caacggctgc
    11641 atctgagcca gataccgctt ccctgcatat gcgaatcatc gaagcgaaac tttgtccggc
    11701 attgccggca ttgaataatc ataacgtctg tccttactgc ttgcgacacg gtgctttcaa
    11761 aaattgcatg atgaatttct gaaagtttct ctgatattat gtcttacagt ttattcgaat
    11821 gaatagtata aatatcagga gaaaagcggt acatttcatt aaaatcgagt ccataaccta
    11881 ccacgaagcc gtcgtcaatg gtaaacccca cataatcggc ctccaaaggc acttttctcc
    11941 tcttccgttt atccagaaaa acacatgttt tgagagattt aggatttctt tccttcagcc
    12001 actccacaaa atactgcata gtgagtccgg tgtccagaat atcctcgaca atcaaaatat
    12061 ccttgtcttt gatggatgtt tcaatatcct tcgtgaggac gactttgccg gagctctcag
    12121 atccggcgcc gtaactggcc agccttgcaa aatccaccct gcagggaatg ttgatttccc
    12181 gaatcaaatc ggccatgaaa ataaaagcgc ccttcagtac gccgatcaaa atcaattctt
    12241 ttcctgcata atctttggat atttgattcg ccatttcccg tacccgtttt tgaatggttt
    12301 ctttgggaaa aagaatcttt ctggacgcct gatccattgc acatcccctt aaattgctta
    12361 taaattaccc aaaagggtat taagattatc caaagtcgtt gtgttatgtc aataaaaatt
    12421 atcctctgag catcagacga aattccggag tgcggctttg catatttctc ggcgtgaaaa
    12481 attcgggcaa cgcatcatac cgtctgttat ttgtactcag caaacctcat gaaacatgcc
    12541 gcttatatgt ggatacaaat ttttcaatat tggcccagat catcttctcc gcttcctcca
    12601 gttggaccgc acttttaaaa gaagaacata gactcaataa aatcgaattc agcacagaaa
    12661 cctctctgcc gaatttttcg tactcgatat gactgatcat gtattgcgag ccgtcaaagt
    12721 aatatttgcc cagacgattc gcttcctgcg accgaaacgg ccagttcagt ttccagccaa
    12781 aataatccag ggcaatttct tttaactcgt catgatcgaa aacgtccggt ttgagttcgg
    12841 cgccgaccct gtcggcaatg agacgaaaat actgaacaac cagatcaata tccgtcagca
    12901 ccagaccata cagataccag tcgtcaatga ttttgatcag aatttgagac tgggactgcg
    12961 gaataaaata atgactggga cagagatgcc ccgcgcacag ttcgcgaccg taaaacgaaa
    13021 catcgcgcat atcgcaccct gcgttttgtt ccggatgcac aagacagccg actttctttt
    13081 catcgtgatc gagaaacccc agatattcgc agcagtgaat cacgtcatat ctctttttga
    13141 aatcttccgc gaccagcgtt tcagtcgcat agcgcttaag atcggctgga ctttttactg
    13201 tttcacggaa acgtttggtg cgcgaacgca gacgccgtgc aagagaagaa cgggagctgt
    13261 ccacgtaatt ataaagtccg caacacgcgc cgcaggattt tcccttatcc ggctgacaaa
    13321 ggacgaaaat attggaattc gacataaata attatccggt ctgtataatg attatcagat
    13381 ctgatgaacc tgtaaaatac ttgttgattg ctctctttca taatttcggc gaaggccgga
    13441 gtctcttttt tgcagcattt tccggacacc ggtccgcacc ggtgtgacgt cttcaaacaa
    13501 cattacaata cttccttact cgaacacgat agtcttcgat ccgtaaacga gtattctgtt
    13561 ttccagatgc aggcgtaaag ccctcgccaa gacaattctt tccagatcct tgctttttct
    13621 ctgaatatca gtcaccgtgt cacgatggct gattttcacg acatcctgcg caataatcgg
    13681 cccctcatcc agctgcgccg tcacataatg gccggttgcg ccgataattt tgacaccgcg
    13741 gtcataggcc tgctggtaag gattcctgcc ggcaaaggct ggcagaaacg agtggtgaat
    13801 gttaatgatc ctgctgggat aatatttgag aaagttgccg ctgagaatct gcatatagcg
    13861 cgccagcacc acgagatcga tatggagatc ccgcagcagc ctcagctctt tcttttcctg
    13921 ctgcttttta ttttttcccg ttacgggaaa ataaaaatac tgcatcccgt actgttccgc
    13981 cagatcgcgg gcatcctcat ggttactgat gaccgctttg atatcggccg ccagctctcc
    14041 catgtcccga cgaagaatca gatcgtgcag gcaatgcaga tgcctggaaa caaaaatcgc
    14101 cgcgcgcggg acgtaatcgg taaaatgcaa atcccatttc atctgatatt gtttggccag
    14161 cggcgcgaac gctacgggaa tgtcgtcacg atccaaagca aacccgttga gctcccattc
    14221 aacgcgcatg aagaaaagct tttccgcctt tgccgtatat tgatcggcgt gcacaatatt
    14281 gccgtcgaaa cggaaaataa aatcggatat tctggccact agtcccttgc tgtcggcgca
    14341 ggacaaaagc aaaacagcat tgacattatt cataaaacat tcaccagttt cctctgcgag
    14401 ctacgtataa aaggattaaa gttgcaggtc aagtataaaa atgttaataa ccgtcttgta
    14461 aaccaaatgg attgagatga gcaaaatgaa actgattgaa gaaataaaat tagataacgg
    14521 gctggattta agaattttcg atttgtcccg ttccatagcg acagatacgg tcaaggtgga
    14581 agtttccttc caaacaaacg ttttactgaa agaatctttt ttcaccagca cggaagatta
    14641 tcgtctggta aaaaatatta tgggcgacga actggcttac gaacacacca tggaaagaac
    14701 gttcgtgtcc aaagacaatg aagattcgac ccgcaatgag ttaatcagca catttaagca
    14761 taattcttta ggatatctct ccgccgcgaa ttttgctcaa aaaatggcat tatccaagct
    14821 cagggaaata aaaagcaatc cgcataaata cagatcccac gctcaatccg acaaaaaagc
    14881 atagatccgg gacctttgcc caaaaaggaa aaaatcatta tgcgccacgg caaaatcgat
    14941 gagcttttat cttccgccga gaaacccagc cgttacatcg gcacggaagt aaacgccgtc
    15001 cgtaaagata aagccgatgt tcgctttctg ctggcctttc cggacacgta cgaagtcggc
    15061 atgtcgcatc tcggattgca gattctctat tcgattatca atgacatccc ttacgcgtca
    15121 gcagaacgtt gttttgcgcc ctggccggac cgggaaaaaa tactgcgtga caaaaaattg
    15181 ccgttgacgt ctttggagcg tcaaatcccg cttcagaatt ttgacatgat cggtttttca
    15241 ctgcaatacg aactttccta taccaatatg ctcaatatgc tggacctggg aggtatcccg
    15301 attgaccgta aagaacgccg ggacggacat ccgctgatta ttgcaggcgg tccctgctgt
    15361 tttaatcccg cgccgcttgc tgattttatc gatgcgttcg ttatcggcga aggcgaagaa
    15421 atcgtcggcg atattgttgc agccgtccgc gaaggcaaaa taaaatccat ctcccgaaac
    15481 gggctgcttg gcgaactggc gaacataccc ggcatgtatg ttcccgccgt tcatcatgac
    15541 aatgaaatca tcaaaaaacg aaccgtcagc gatctcaatg cctggaggca tcccctcaaa
    15601 cccgttgttc cgctgatgca aaccattcac gaccgcatca cgctggaaat cgcgcgcggt
    15661 tgcacccgcg gctgccggtt ctgccaggcg ggaatgatct ggcggcctta tcgcgaaaga
    15721 aacgccgccc tgcttatgga aatggctgaa aagatgctcg cggcaaccgg ccatgaagaa
    15781 atatcgcttt tatccctcag ctccggcgac tacagttgtc tagaaccttt ggtaaagggc
    15841 ctgatgaacc ggtattgtca aaagcgggtg gcgttggccc tcccttccct gcgtgtggaa
    15901 tccctcaaca cgactttaat ggaagaaatc aggcgcacgc gcaagaccag tttcaccctc
    15961 gcgcccgaag cgggaacgga caaaatgcgt ctggttatca acaagggcaa cacctccgaa
    16021 gatttacttt ccaccgcgga taaaatattt gccgccggct ggaaatccat caagctctat
    16081 ttcatgctgg gcctgcctca tgaaacacag gaggactggg aaggcatcgt caatctcagc
    16141 catgaaaccc tgcgcgcggc aaaaaaccgc ggtcaggtta ccatcagtct ttccaccttc
    16201 gtccccaaac cgcatactcc ctttcaatgg gaaaaacaga tttcactggc agaaacttat
    16261 gaaaggcagg atttcatccg cacgcggatc aaaaaccgca atctcagcgt caaatggcac
    16321 gacgcgcgga tgagttttct ggaaggcatt ttttcgcgcg gcaatgagca agcgggaaaa
    16381 cttctgggaa atgcgtttcg caaaggttgc cgcttcgacg gttggtctga tattctgcgt
    16441 ttcaacctct gggaagaagc catcgccgag tcggacattc atcccgacga ctaccttcgc
    16501 gaacgcggga ctgacgaaaa attgccatgg ggcaacattg actgcggcgt gagccgtgaa
    16561 ttccttttgg aggaaaagca gaaagcgttt cagcagacgg caacagcaga ttgccggttt
    16621 gagacttgcc ggaattgcgg agcatgcgat ttttcgatca cgaaaaatat tctcgccgca
    16681 gacaacgaaa acaaatctgt gttgccatcg tctttggcac ccgagacttc ttcggcacaa
    16741 gaagaaaaat atcgggttat gtttaataaa atggaaagcg ccggattcct gtctcatctg
    16801 gaactagcca ccgctctggt tcgcgccttg cgccgcagca gcatcaacct tgcttatacc
    16861 ggtggttttc atccgcatcc caagatttcc tttgccacgg caaccgctgt aggcatggaa
    16921 agcagacagg aatatatgga catcaccgca caggaatatt catgcagtct ggatttatta
    16981 agaactgaaa tcaacactct gcttcccaca ggtattaaaa ttttggaaat cagaaagcta
    17041 tctccggaag aaaatgctat cgctcaagcg acaaggggat tcgaatatga actgcaactg
    17101 cctgccgcta ccgacgctcc ccgaatgagc gccataaaag caaacatcga tcaatttctg
    17161 gcggcatcca atttcaacat caagaaaata tccaaaggga aaaccataac caaagacatt
    17221 cgtccttttg tgcaaatgct ttccctagac cgtgacaaga aaataataaa atttatcgta
    17281 tcctttgttc aggaaggttc ggccaggcct gtcgatatca tcgcccacat cctgaaatcc
    17341 gaccccgacg aatcgcgtca aatcagggtg gtcaaaacca aaacactgct cggatagctt
    17401 tatcaataag gagagcttgt gagaatttgg gacgtcaatc ccggttattt aaaccggcaa
    17461 agcttattgg gtgaacaccg cgagctgcac gcgattgttt ccatcatcca gcacaataaa
    17521 aaaggatatt cgcggcatcc ggaaacgctg cgctggcatg gtttcggctg ggcattgagc
    17581 cagcgccaca gattactggc ggcggaaatg aatctgcgcg gtttcacgga caggtcgccg
    17641 gcgatgatca aaacgcaacc gcaaaaatgg ccgcatgtat ttgtcgattc tccgacgtct
    17701 caattctcca tcctgaaaaa gaaatacgtc aacaaagaac agggacgcat tccgttgccc
    17761 aatagtgttc agcagctatg ggcgcagcat caatattcgg tcatggcgcg cgatgaagct
    17821 gaatacaaat acatcggcgg ttgggtgaca tcccggaaaa gcggcaaggg aatccatgag
    17881 atttatccgg aactggtttc gctgcttcgc tgcccgcccg tcgaagccaa tctcagaaac
    17941 gctcttgaac agatgtggaa tttcgtttgc gcgtacgcgc cgcatcccgc aaccgccatg
    18001 aaaaaagaat cttcacgaca tcttttgaag cagatacagc gcacggccgt tttgcatgat
    18061 attgtctacc tgaaagaatc aacggcgctg ggtgaattgc cggcgtggca gattggatga
    18121 aacaatcatc cgtatctttt attttttcct cgaaccgcaa tggtctttct gcagtttctt
    18181 ttgctcttga cgcagcatct tgaaaaacgt ctcccgcatc ctcatgggaa ttttgctcac
    18241 cctgtacccg ctgcaaatcg ccagaagata cgccagagaa tatctttcca gaatttcaac
    18301 attatgaacg gcgcgatcca tgtcgtgacc taaaaccagc gcgccgtgat tctgaatcag
    18361 gaaggcattg ttgccgtttt tgatgtgtcg gagaacattg tttttcagag gcacggatcc
    18421 cgaaggagca taaggaatga ccgccaccgt tttgcccaga aaacgcacct gctcatcaaa
    18481 cagcgccggc agcggcgttt tgataattgt cagcgcgccc gcataaacct gatgtgtgtg
    18541 gataacggcg ttgacatcgc cgcgtgttct ataaacggca ccgtgcaaag ccgcttccat
    18601 ggatggtttc catggccccg aaagcacgtt gaggtgttca tcaagaatac aaatatcggc
    18661 cgcggtcatg gtcatgtaat tgcgattgga cggtgttatg gcaaaagcat ttcctttttt
    18721 aatccgcacg gaaatattgc cgccggttgc cgcaagataa cccttttcaa caagcttttg
    18781 cgctgttttc acgatcagac gcctgtctga attaaattgc ctcatggagt ttctccattc
    18841 caaatagcat taagatcagc cgagtttttt ggactctacg gattattttt atttgcggcc
    18901 aggtcaaaaa ggcttggctg aacgggggaa tcagttcact aaaaaatcag tttaagttct
    18961 ttgggtaaaa tcctgaagga aaaattgttt cgcttttctt tttccggatc gccgttgcgc
    19021 tgcagcaatt gctccgttga agtggtgata tcaatttctt tcgctttgta atactctccc
    19081 attctgttcc cccccgcaag agaagttgcc aaagcataga gcgtgccgat gggtccggca
    19141 ttcaggatca gcaaatgcaa atatccgtca tcaaattgag catgagggac aaccttgaat
    19201 ccgtagccgt agtagggaag ttttgtaata acggcggtca gagagttgaa ggttcttatt
    19261 tcctttttgt ccatcaaaac acgcaaatca gccttgatgt atccgtcaaa taaaattttt
    19321 aatgtcgaca gaccataggc ggtaaaaccg gtcaaattat tttccagata gttatcgcgt
    19381 tctaacagaa tacttccttc aatgccgata ctcgcgaaca aggcttttgt tttcccgtcg
    19441 cagcaaatga catccagcaa atattctttg cctttctgaa tcaacagagc attttctttg
    19501 ggacaacggg gaaggttgaa ggtatggcgc aggacatcgc cggaaccgaa cggcagaaat
    19561 cccagaggga tgtctctgga aacgctgttc atcaccagtg aaaaggttcc gtccccaccg
    19621 gcgacaagca gcctggccac tttttgggaa aggtcatgcg ctgcagtgct gaattcttcg
    19681 ggagattttg tatcgagacc ggcaatggtg gcgtctttta aaataccggc tacttctttt
    19741 aaaattttga ttttgtcatc tacagagcgt ttgccggaaa cagggttcgc gataatgccg
    19801 tatttcatgt caaatcatct cactgccaca ttgtattttc gaacgaggag caagttttga
    19861 tggagcaaat ataagtgccg tgatttttta tgtcaagaaa ataatcgcgc accaacaaac
    19921 gctattttct taattgacga atttcctaaa gtttgtccag cccgaacttc cgggaaagca
    19981 gtctgtccgt aaacgtacgg ggcaatttgg cgacaaaggg atacatcgtc gccccctttc
    20041 cgatgcggat gattctcggt gttttacttt tcacgagttt gtcgacaagt tgtctcgcga
    20101 agacgtccgt gggtgtcgca ccctgttgcg acatgtgcgc acgatcgaca ataaaatcgg
    20161 ttatcggggc atagggcgtt tttcggaaac gttcaagccc tttgtcggcg ttatccgata
    20221 aatttgacct gatgccgccc ggttgaacgg taatcacgtc gatcccgaaa ggtttcagtt
    20281 ccatgcgcaa cacatcggaa taggaggtca ccgccgcttt ggtggaacag tacgcgcccg
    20341 aaaaaggcgt attcagaaca ccggagatgc tgctgatatt gacaattttt cccgatgttc
    20401 ttttgatcat gtgcggcaca accgcatggg tcaaagccgc cagtccgatg acgtttgttt
    20461 caaactccct gcgcaactcg ctgatttcca gttccgacaa ggggccgatg agcagaagac
    20521 cggcgttatt aatcagcatg tcaattttac cggcgttggc catgaccgat tccacgcagg
    20581 ttttaatcga tttttcatcc gttacgtcga gggcggcggt ttgcacattt ggcgctttca
    20641 gatcctcaat ggccgcatgg ttgcgggcag tggcaaacac ctgccagccg cgtttcgcca
    20701 gttccgtggt caggaaacgt ccgattcccg ttgaacatcc gctgatgagc gccactggtg
    20761 cgccagcatt catgataaac ctccttggtt gaactgtatg attttaacgg cacatggttc
    20821 ttacagtata tggttgtgtt cgtatttttt tcttatttca taactgcgca gaaaaataaa
    20881 cagtattttt caaattcgtt tttgccccga catggtgaaa ttgtttcttg ccagtgcacc
    20941 gtatcacggc ataaaatgtt tctatttttt tacttttcat atgatatcag caacttatcc
    21001 atttttcgca gaaatagaaa tatttctcct gtccggatta aaatttattg acaaacgggg
    21061 acgttttgtc tataaagatc agtcttaatt caattttcat aaggatacgt tatgtacgca
    21121 gtgattaaaa caggagcaaa acagcacaag gtaagcgaag gcgacatttt atccgtcgag
    21181 aaactggacg ggaataaagg cgatgaagtc gttttcagcg atgtgctgat ggtctccgac
    21241 gataaagaga taaaagtcgg caaacctttt gtggacggcg ccaaagttgt cggtgaaatt
    21301 atcgcccaga agaaaggacc gaaacttact gtttaccata tgaaaagacg gaaaggtttc
    21361 cataaaaaaa ccggtcatcg tcaaaatcta accagcatga aaatta
//
LOCUS       DAOSME010000040        20837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3150_length_20837_cov_23.303917, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000040 DAOSME010000000
VERSION     DAOSME010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20837
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3150_length_20837_cov_23.303917"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLG98_07225"
     CDS             complement(<1..320)
                     /locus_tag="PLG98_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33084.1"
                     /translation="MTEMFNKISYHDLNAKQKEQFNFQKISAVLADYGFLTIKIGDDW
                     KGADFLAQHVGGDMILKVQLKGRLWFEKKYIGKNIWMCFRKDNFVYLYPHDDLLNIML
                     ISTN"
     gene            complement(335..769)
                     /locus_tag="PLG98_07230"
     CDS             complement(335..769)
                     /locus_tag="PLG98_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33085.1"
                     /translation="METNTDSAKELTTRWLKFYTYVVLPFKIITSPVTILADYNRMLR
                     AGYNVSLDPTAFIPVVIFDIFICFLIYGLHKRLLWGWICNWVFLGLMIFSALNSRISF
                     GANVVAIILIFLVFFVPNYFYFKKRKYLFMSGLPEDNKIITN"
     gene            complement(1194..1538)
                     /locus_tag="PLG98_07235"
     CDS             complement(1194..1538)
                     /locus_tag="PLG98_07235"
                     /inference="COORDINATES: protein motif:HMM:NF022841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3192 domain-containing protein"
                     /protein_id="HOE33086.1"
                     /translation="MKRLMFLLLILLLVSGCGLVTLTTTSIEASGNKSNLQKLEIGMN
                     LTQVKEIMGAPNKTEAFSLPENKKLLVWYFLTEGRAPFRRLDDPNYTPLIFENDILKG
                     WGYAHLDKMRKQ"
     gene            complement(1733..2059)
                     /gene="cutA"
                     /locus_tag="PLG98_07240"
     CDS             complement(1733..2059)
                     /gene="cutA"
                     /locus_tag="PLG98_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOE33087.1"
                     /translation="MKSYIQVSTTTETKEQAQKIAKYLVEQKLAACVQISGPIESTYR
                     WKGKVETAKEWLCLIKTLTTLFKKVEVAIKKLHPYETPEIIAVPIIKGNREYMNWLDD
                     ETENLS"
     gene            2597..3439
                     /locus_tag="PLG98_07245"
     CDS             2597..3439
                     /locus_tag="PLG98_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOE33088.1"
                     /translation="MKSKIKILVFLCLAISVMFSLVNCRQQNGVCDCPPSKFGNGIYY
                     SQTYEDYILAHVFQDVADGFYIDVGANDPNIFNVTRFFYERGWHGINIEPNAELYLQL
                     RSFRQRDINYNVGIATTEGRMIFYQQAYQSDALMPLSTFDKEIVDIQKKNGILFKEIV
                     VPVTTLNNLLGKISVREITFITIDVEGFEKQVLESIDLARYKPVVLCIEASRPGTGDL
                     SYMLWEQIVLNNNYVFAMSDGVNRYYLHKSHIELLPRFIDIDRCVKLSKYKRQVKMDG
                     WSSW"
     gene            complement(3562..>3914)
                     /locus_tag="PLG98_07250"
     CDS             complement(3562..>3914)
                     /locus_tag="PLG98_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOE33089.1"
                     /translation="SIILSANSVDFTNPKAIRASMGSFFHLTMIPEVDLIEALPEIRK
                     HFFVIGSDVHEGTAPHPVQKNAALILGNESHGLPGTILKMTDEKWSIPGSGKADSLSL
                     PQAAAIMMYECTKK"
     assembly_gap    3915..4014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4015..4496)
                     /locus_tag="PLG98_07255"
     CDS             complement(<4015..4496)
                     /locus_tag="PLG98_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOE33090.1"
                     /translation="MQNGFLTPSQIQLKKWARLDDAKARHEEGVFLAEGVKVVEELLK
                     SDWQIKALLVMPEKIKFWKKLDLSREEEIPVYQLKRTQWEKIGQDREPEGIMALVEMK
                     QAPDFSSWLAQTSGNVLILHEINNPLNLGALARSAQWFGFNSIILSANSVDFTNPKAI
                     R"
     gene            complement(4501..5439)
                     /locus_tag="PLG98_07260"
     CDS             complement(4501..5439)
                     /locus_tag="PLG98_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOE33091.1"
                     /translation="MTLWWTIFGVFILAMLALDLGVFNRKAHVIKMKEALLWVSFWVS
                     LAVAFGVGVYYFYGHSKALEFFTAYIIEYSLSIDNLFVFLLIFRFFNVPQAYEHKALF
                     WGILLALITRAAFIFAGVALINKFHWIMYLFGAFLIYTGIKLAMNKQTEVHPDKNIAI
                     KMLRYVVPVTKEFHEARFFIIKNGKRFATPMLAVLLALETTDILFAVDSIPAVLAISR
                     DPFIVYTSNVFAILGLRSLFFAISGLMKLFHLLHYGLSAILSFVGVKMLIADFYHIPV
                     GISLTVISSILVISIVASIIWPDTKYEDIPNITMKD"
     gene            complement(5458..6789)
                     /locus_tag="PLG98_07265"
     CDS             complement(5458..6789)
                     /locus_tag="PLG98_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE33092.1"
                     /translation="MGKHLIIVGGGAGGASAAAEAKRNDPELNVAILQSGKFIAYASC
                     PTPYYIGDIIKDENRLVARTPEEFRKRGVEVQLDTEVVNINCNANVVETRDNLMLPYD
                     YLVIGTGTNPFVPDIPGRELPGVYVLNNLENAIHIKAWLRERKAKRVVIIGGGFIGLE
                     MSEALHEVGISTTIIHKHSLPANRWSKELSSVMLEELQSHSVEFIGNAQAKAIEKSSG
                     DSLKVITDAGEWQGDMVLLAVGVRPNAALAKSAGLTIGKTGAIAVNFAQQTSVENIYA
                     VGDCCESWNRVSRRWVNIPLGDIANKQGRVAGRNIGGNPLTFDGIVGAQSFRLFDLEC
                     AATGLSEKEALAAGFVPISNITWGSAMAPAMGMKKIGLKLTADKSSGKLLGAQAVGVA
                     GAVGRINALSVALWTGLNIDEVGYLDLAYAPPFSAAWDIIHNAAQALRRMI"
     gene            complement(6803..7636)
                     /gene="lgt"
                     /locus_tag="PLG98_07270"
     CDS             complement(6803..7636)
                     /gene="lgt"
                     /locus_tag="PLG98_07270"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOE33093.1"
                     /translation="MEAFINTWQQLPSHINPALFTIGSFQLRYYSLMYLVGFAIVYLL
                     TTYRISNEHYEYKKEMLQDYLIWGMFAMIVGARLGEVLIYNFSYFSQHPLEIFLPFSF
                     SNGIHFTGISGMSFHGGVVGVMIYSIYFLRKRQINFWRFADLIVPSIPLGYTFGRLGN
                     FLNGELWGRVTTMPWGMYFPFDPTQHLRHPSQLYEAFFEGIFLFVILWLLRKKKFADG
                     FLLGLYIFGYGFVRFMIEFFREPDFMVGSVSIGQVLCVLMMAAGLIIVLWRRQIASPA
                     R"
     gene            complement(7766..8728)
                     /gene="trxB"
                     /locus_tag="PLG98_07275"
     CDS             complement(7766..8728)
                     /gene="trxB"
                     /locus_tag="PLG98_07275"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOE33094.1"
                     /translation="MSKANLPQYDCDTIIIGGGPGGYTAGIYAARSGLKSLLVEGAST
                     VSQITITDLIENYPGIPDGINGFDLMQLFKKQAQNFGLEIIANDVHSIKKAGDNPAIW
                     EIAVGDKSYRTLSVIAATGAQWNKLGVPGEEEFAGKGVSYCATCDGPFYRNRDVVVVG
                     GGDTAIQEALFLTHFAKKVSVIHRRDRLRAAAILQKRAFAEKKIEFIWKAKLAEIAGQ
                     EFVTGVRVADVESGKINHIEAEGVFIFVGLTPNTALFRDVLNLDKGGYIITDDNMRTS
                     APGIFAAGDCRAKQFRQVVTAAGDGANAIYSAELYVDELKGQTY"
     gene            8961..9260
                     /gene="yhbY"
                     /locus_tag="PLG98_07280"
     CDS             8961..9260
                     /gene="yhbY"
                     /locus_tag="PLG98_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOE33095.1"
                     /translation="MEKLKGAQKKYLRAQAHHLKPVVMIGAKGSTTQLISSVNAALND
                     HELIKIKFGEFKEAKKEISQEIAGKTKSELVGLIGNIAIFYRQNPNPERRKIKLS"
     gene            9293..9613
                     /locus_tag="PLG98_07285"
     CDS             9293..9613
                     /locus_tag="PLG98_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33096.1"
                     /translation="MSSELLRYEINRKIRQVLVSHNADMTKISYSFVHRTVYMSGNLV
                     RESQGEFSLPVIEGMIRELMKLPRVQKILFDLENWIISNEPGALNIVKKKGLGQHPAI
                     KDSV"
     gene            complement(9877..11643)
                     /locus_tag="PLG98_07290"
     CDS             complement(9877..11643)
                     /locus_tag="PLG98_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOE33097.1"
                     /translation="MNNTKIPTVKPFVHHLRPRGLSRRIPLIETLRNYRREWLMHDIL
                     AGLVLTAILVPVGMAYASVSGLPAIYGLYATIIPLIAYAIFGPSRILILGPDSALAGL
                     IAAAILPLAAGNTDKAIALAGLLAILAGAFCILAGLVKFGFITDLLSKPVRIGYLNGI
                     ALTVLIGQLPKVLGFPVGGNNILQEVNSLIQGIAGGQINWMSCLIGCSCLMIILGCRQ
                     WFSRIPGVFIAVAGATIISSWFHLSASYGIAVVGPLPQGLPQFQIPSVSFAEFKALFA
                     AAFAIALVSFADMSTLSRTFALRAGQEVDSNQEIIALGAANMAAGLFQGFPVTSSASR
                     TPVAESAGAKTQVTGVVGAVCIALLLIFAPNLLKNLPQAVLGAVVICACISIVEVRAL
                     ARLYKLRREEFVFSIACFLGVVSLGIIKGIFIAIGLALFSFIWRAWHPYDAVLGHVEG
                     LKSYHDISRHPDARLIPGLVLFRWDAPLFFANAEAFRENVLHAIAEAPTPTKWVVVTA
                     EPITDVDTTAADMLAELDNVLHESGMALFFAEMKDPVKDMLKRYGLFTRFGEENFFPT
                     IEQAVERYLTLHKIDWPESHKR"
     gene            11755..12090
                     /locus_tag="PLG98_07295"
     CDS             11755..12090
                     /locus_tag="PLG98_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOE33098.1"
                     /translation="MPFKTLITVLICNFIFVMIAVPLALRKVPKNVIYGFRIKATLEN
                     DFVWYEANAYFGKVFIIGNMVCALLILILYFSDVVPAQNFVNIGMAILIIPALVAALL
                     TFRTIKTKH"
     gene            complement(12141..13460)
                     /locus_tag="PLG98_07300"
     CDS             complement(12141..13460)
                     /locus_tag="PLG98_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOE33099.1"
                     /translation="MNKKRLVLLVILFITIGLFSLQQPSENSFRGDAVVSEPTAPEKG
                     TRPLKSISDKFEKGETLSSIFKKYGMHTDDLFELKSAVASIQSLRDIKPGKPYSFTLN
                     EDNYISSFTCGIDDDTILKIERVDGCFQAQKYNIPYETRILTIGGTIQNTLVSAFGNE
                     REDYLLALQAADILAWDIDFNTDVRKGDTFRIIVEGLYSEGKFKKYGRILSIEFVNGS
                     QTYNAYLFEQDGKTDYYNADGQSLRKAFLKAPLSFRRISSHFSRSRLHPILKVRRPHH
                     GIDYVAATGTPVSTVADGRITFAGYKSGYGKIVIVNHANGYQTYYGHLSRIKSNIRPG
                     KKVQQGDLIGNVGATGLATGPHLHFEMRQGGRAINPLAVKKVAGSPVPAAQMAEFKNT
                     TQTMNNIYTVALFFDTNNTQRKNHSLQPALLYASLASSSPQGKFRKN"
     gene            complement(13785..15470)
                     /gene="ettA"
                     /locus_tag="PLG98_07305"
     CDS             complement(13785..15470)
                     /gene="ettA"
                     /locus_tag="PLG98_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOE33100.1"
                     /translation="MANEGNKVIYSMIGVSKIYDKKPVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLKILAGVDQEFLGKTILSPGYTVGYLEQEPHLDNDKTVRQIVEQGVQSTMD
                     LIQEYNDINEKFAEPMSDDEMAKLCERQGAVQEKLDALDAWDIDSRLEMAMDALRCPP
                     GDTFVKVLSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQKYEGTII
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYSSWLEQKQNRLKNEEKAESDRIKTLER
                     ELEWIRMSPKGRHAKSKARISAYEELLGKTMEKESGTREIFIAPGPRLGDLVIEAKDV
                     NKGYGDKLLVEGMTFSLPPGGIIGVIGPNGAGKTTLFRMITGREKPDAGTIRIGDTVK
                     LAYVDQSRDSLDPNKNIWEMISDGQDIIAIGNRQINSRAYVSRFNFSGTDQQKKVGTL
                     SGGERNRVHLARMLKEGGNVLLLDEPTNDLDVNTLRALEDALENFAGCVVVISHDRWF
                     LDRICTHILAFEGDSKVLFFDGNYSEYEEDYRKRYGNAASQPHRIKYRQLTRR"
     gene            complement(15499..>15684)
                     /locus_tag="PLG98_07310"
     CDS             complement(15499..>15684)
                     /locus_tag="PLG98_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE33101.1"
                     /translation="DQSVGRTDFPGGNGDLLKKSILAFAKLDLEWLLPGHMGILHGKS
                     SIQNNFNLIIKGIFPYI"
     assembly_gap    15685..15784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15859..16071)
                     /locus_tag="PLG98_07315"
     CDS             complement(15859..16071)
                     /locus_tag="PLG98_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOE33102.1"
                     /translation="MNERNWLQNLTRSNTDKWIGGVCGGLGESSPVPAWAWRLLFVTL
                     VLCFGTGVLLYILLWIFVPDKSTIQE"
     gene            complement(16095..16604)
                     /locus_tag="PLG98_07320"
     CDS             complement(16095..16604)
                     /locus_tag="PLG98_07320"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE33103.1"
                     /translation="MMNKKILLAIALVFVFALVAFSFEQFKPSVFTRRDKVSAPDFSL
                     KDIHGKTFRLSAQRGNPVIMFFGTTWCPACREEMPLYRNLYNKYAGRGLKFLYIDINE
                     SAERVARFAGQNAFPYHVLVDGDGSVAGDYNIIGVPTLILLDREGNIVDVGHRISDLP
                     VDRLFSVKK"
     gene            complement(16614..17330)
                     /locus_tag="PLG98_07325"
     CDS             complement(16614..17330)
                     /locus_tag="PLG98_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOE33104.1"
                     /translation="MIEPLVNNLSFYLESSLMLSVLAAYLGGVIISFTPCTYPLIPVT
                     VGLIGIQGPSSKIRGFFLSLFYVAGMAVTYAVLGAAAALSGKIFGQMQTTPWTYFIMA
                     NLCLVMGLSMLDVFNLSIPVPQKLMQWTGANGKKGFLNSFLIGAVSGVVIGPCTAPAL
                     GVLLGYVAVKTNVVLGMSLLFIFAFGMGTLLIIAGTFAGMIAALPRSGAWMEKIKFIS
                     GLILIGAAEYFLYIAGRLSI"
     gene            complement(17449..17535)
                     /locus_tag="PLG98_07330"
     tRNA            complement(17449..17535)
                     /locus_tag="PLG98_07330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17499..17501),aa:Leu,seq:cag)
     gene            17652..18602
                     /locus_tag="PLG98_07335"
     CDS             17652..18602
                     /locus_tag="PLG98_07335"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOE33105.1"
                     /translation="MIIFRSTENITGDFRGSFVSIGNFDGVHLSHQFICRKLASEAKQ
                     AGTKSLVITFDPHPKMILHPNIRPFFLITTLDEKLERLAACGIDGVLVIPFTMEYSKT
                     TAEEFVHNFLGRKLAIGKIIIGHDYTFGQGKKGNSDYLIAAGRDMGFAVEVVDAVKSG
                     NDIISSTLIRKSIIEGNLKKAAVLLGRYYNVAGKVVTGFGRGVTLGFPTANIEPEKEL
                     LPPPGIYAAFVDAEEKRYQAVLNIGAKPTFEDYTFSFEVHLLDFSGDLRGKRINTDFV
                     EKLRDIVKFDSPETLKKQIAADVEKAKTILLENISNRQGT"
     gene            18619..19701
                     /gene="asd"
                     /locus_tag="PLG98_07340"
     CDS             18619..19701
                     /gene="asd"
                     /locus_tag="PLG98_07340"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOE33106.1"
                     /translation="MLKIAIVGATGIVGQQAIVSLLGHPWFKIAKLVASERSAGKKYI
                     DALRDANGSIRWWCPEELPGHIANMVVEEAARFDPKSVDIIFSTMDAGPARDLEPKYA
                     KTTPVISTASAFRYEPDTPILVGGVNMDHAALLAVQQKNRGWKGFISPKSNCTIVGLV
                     VSLKPILDNFGVKQVVATSLQAMSGAGRTPGLSAMDMTENVVPYIPGEEPKVETEPLK
                     ILGKLTGKIIVNAAFGITATCTRVPVLDGHMVCAFVQTKKKCSPEDVKKAMMNYGKDF
                     AELHLPSSPAHLIDVTDDPFRPQPRMDRDKGGGMTVTVGRIRKDPVIENGIKYVCLAH
                     NTKIGAAKGALQTAEYLVKNYLKWVK"
     gene            complement(20095..20601)
                     /locus_tag="PLG98_07345"
     CDS             complement(20095..20601)
                     /locus_tag="PLG98_07345"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOE33107.1"
                     /translation="MTQLKAVQANIVTLKVDAIVNAANQSLLGGGGVDGAIHRAAGPE
                     LLAECRTLGGCKTGEAKLTKGYNLPANFVIHTVGPVWHGGKSGESQLLASCYRSSLIV
                     AAENGIKTVAFPSISTGVYGYPVEPAAQVAVKTVRDTVQKLSTIEEVIFCCFSGKDLE
                     IYEACLKN"
     gene            complement(20674..20823)
                     /locus_tag="PLG98_07350"
     CDS             complement(20674..20823)
                     /locus_tag="PLG98_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOE33108.1"
                     /translation="MGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAV
                     EQAMK"
BASE COUNT         5448 a         5012 c         4913 g         5264 t
ORIGIN      
        1 atatttgttg agatgagcat aatatttaaa agatcgtcgt gcggatagag atagacaaaa
       61 ttatctttgc gaaaacacat ccatatattt ttcccaatat attttttttc aaaccacaac
      121 cttcctttaa gttgtacttt taaaatcata tcgccaccaa catgttgtgc taaaaaatca
      181 gcacccttcc aatcatcgcc gattttgatt gttagaaaac cgtaatcagc aagaacggca
      241 gatattttct ggaagttaaa ctgttctttc tgtttggcgt taaggtcatg gtaagatatt
      301 ttattaaaca tttctgtcat ttctttcacc atttttaatt ggtaataatt ttgttatctt
      361 ctggaagacc tgacataaat agatattttc gtttcttaaa atagaaatag ttaggcacaa
      421 agaataccag aaatattaaa attatagcca ccacattggc accaaatgat atccgagagt
      481 ttagtgcact aaaaatcatt aatcccaaga atacccaatt acaaatccat ccccataaaa
      541 gtcttttatg aagtccataa attaagaaac aaataaaaat atcaaaaatt actaccggaa
      601 taaaagctgt tgggtctagt gatacattat agccagccct aagcatcctg ttatagtcag
      661 ctaaaatggt tactggagag gttatgattt taaaaggtaa aacaacatag gtatagaatt
      721 tcaaccaacg cgtagttaat tcttttgcag aatctgtatt agtctccatt ttaatcaccc
      781 tccttatcgt tatgctaacg cggaattcag ccggagcgta ggcgatcggc tgaaatgaat
      841 cgttatgttt ttttacagtt ttggtgtgta tacatctata tatgtacgcg agcaatttgt
      901 ttcatggccc caacaacacg cgtaaatgta tattgagtat tcgcctttat tgccggttcc
      961 ctttattctt atacaatctt gaccattgtg tttgccacct tgcgtaacgt gcgcttctgc
     1021 agtgaatgga gtcccatagt ttgtagacag atgtcgtggt cctgtattca gtaaatgttg
     1081 tctagccgca acgaatgttt tttgattatt tactgttccg ttatgtggtt gtcttgttgg
     1141 tctgctcata gaattcaccg catatatagg cgttttgttg catattaccc tttttattgt
     1201 ttccgcattt tatcaagatg cgcgtaaccc cagcctttaa gaatatcgtt ttcaaaaatg
     1261 agcggggtat aatttggatc atctaaacgt cggaaaggtg cccttccttc agttaaaaaa
     1321 taccaaacca aaagcttctt attttcaggc aatgaaaaag cttccgtttt atttggtgcg
     1381 cccataattt ccttgacttg ggttaagttc atcccaattt caagtttctg gagattgctc
     1441 ttattgcctg atgcttctat tgatgttgtt gtgagagtca ctaggccgca tccagaaact
     1501 aataacaata tcaacaaaag aaacattaat cttttcatga tgcacctcta aacataacat
     1561 tgtttatata gatctatgtt aatattgtaa acgtcgacaa ttccttctgt ataaaccccc
     1621 actacaaaaa ccgcaatttg agccctagta acttaatatt aaacgataat caatctattt
     1681 cctgtttgtt tactggagat attatacaga tctgcataat atctcacatc gtttacgaga
     1741 ggttttccgt ttcgtcatcc agccagttca tatattccct gttgcctttg atgataggta
     1801 cggcgatgat ttccggtgtt tcgtagggat gcagtttttt gatggcgact tcaacttttt
     1861 tgaataaagt tgtaagggtc ttaatcaggc agagccattc ttttgctgtt tccacttttc
     1921 ctttccaacg gtatgtactc tctatcggac cggaaatctg cacgcaagca gcaagttttt
     1981 gttcaacaag atatttggcg attttctgcg cctgctcttt cgtttcggtg gtcgtggata
     2041 cttgaatgta ggatttcatt gtttcaattc cttattatca ttgcaaaact agtgaagcaa
     2101 aacatcccgc gtcgctacgc tcctgggata attccactta cgcttcgaac atcgcgtagc
     2161 tcgttcttag cgagtgtcat tagattcccg ctaggcccat gcgggaatga cagtggggtt
     2221 gcccttttat ccattcacga tgatcctact ggacatcggc cttcgccggt gtgacgtttt
     2281 ttagttaccg tttaacacat tcatacatca tgatcgccgc agcctgcggc aaactcagcg
     2341 aatcggcttt ggacgatgaa caaatctgtc attatttgca ttggatagca tattatttgt
     2401 ttgaccttac gaaatcaaga aaaaacaaac aacaaagaaa ttgttttagc aattacaccg
     2461 atccggtttc atattattca tggaatcgtg aaaaggcatg aatttcagaa aaataaaaga
     2521 tggtattttt tgatgatttg tcgcaagata tttccatccg aaatcataaa aaacaaatta
     2581 cgcgaggcgg aaaacaatga aatcaaaaat taaaatcctt gtttttttgt gccttgcgat
     2641 ttccgttatg ttttctctgg tcaattgccg gcaacaaaac ggggtgtgcg attgtcctcc
     2701 ttctaaattt ggaaacggga tttattacag ccagacttat gaggattata tactcgccca
     2761 tgtttttcag gatgtcgccg atggtttcta tatcgatgtc ggagctaacg atcctaatat
     2821 ctttaatgta actcgattct tctatgaaag gggctggcat ggcatcaata ttgagcccaa
     2881 tgccgagttg tatttacaac ttcgctcttt tcggcagcgc gatatcaatt ataatgtcgg
     2941 tattgccaca actgaaggac gtatgatttt ttatcagcag gcataccaat cggatgcatt
     3001 gatgccgctt tcaaccttcg ataaggaaat cgtggatatt caaaagaaaa acggaattct
     3061 tttcaaggaa attgttgttc ctgtcacgac acttaataat ttgcttggaa agatatccgt
     3121 tcgtgaaatt acattcatca ccattgatgt tgaaggcttt gagaaacagg ttctcgagag
     3181 tattgatctt gcgcgatata agcccgtggt gttgtgtatt gaagcgtcgc gtccgggcac
     3241 gggagatctt tcctatatgc tttgggaaca gatcgttctg aacaataatt atgtgtttgc
     3301 gatgtccgat ggagttaacc gctattatct tcataaaagt catatagagc ttctgccccg
     3361 cttcattgat atagacagat gtgtgaaatt gagcaaatac aagcgccagg tcaagatgga
     3421 cggttggagt tcatggtaat gacatgtcaa aataattttt tacgggcagg cagcctgccc
     3481 ggcgaatgcc tcggaaacct ataatatact tcaaaaccgg attccggtct tccccggaag
     3541 aacaatattg tttgcttcgg tttatttttt tgtgcattca tacatcatga tcgctgccgc
     3601 ctgcggcaaa ctcagcgaat cggccttgcc gctgccgggg atgctccatt tttcatcggt
     3661 cattttcaaa atcgttcccg gcaggccgtg actttcgttg cctaaaatca gagcggcatt
     3721 tttctgaacc ggatgcggcg ctgttccttc atgaacatcg gaaccgataa caaaaaaatg
     3781 ttttctgatt tccggcaacg cttcgattaa atccacctcc gggatcatcg tcaaatgaaa
     3841 aaagctgccc atcgaggcgc ggatggcttt cggattggtg aagtccacgg agttggcgct
     3901 cagtatgata ctgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggatg
     4021 gctttcggat tggtgaagtc cacggagttg gcgctcagta tgatactgtt aaatccaaac
     4081 cactgcgcgc tgcgggccag cgccccgaga ttgagcggat tgttgatttc atgcaggatc
     4141 aaaacattgc cggaagtttg tgcaagccac gaggaaaaat ccggcgcctg tttcatttct
     4201 accagcgcca tgatgccttc cggttcccgg tcctgtccga ttttttccca ctgtgttcgc
     4261 ttcaactgat agacgggaat ttcttcctcc cgggaaagat caagcttctt ccagaatttt
     4321 attttttccg gcatgaccag caatgcttta atttgccagt cgcttttcag cagttcttcg
     4381 acgaccttga cgccttcggc cagaaacacc ccttcttcat ggcgcgcctt cgcgtcgtcc
     4441 agtctggccc attttttcaa ctgaatctgt gaaggcgtca aaaatccgtt ttgcatggcg
     4501 ttaatccttc atggtgatgt tcggaatgtc ttcatatttt gtatccggcc agatgatgga
     4561 cgcgacaatg gatatgacga gaatggacga tatgaccgtc agagagattc ccaccggtat
     4621 atggtaaaaa tccgcaatga gcatctttac gccgacaaaa ctcaaaatgg ctgaaaggcc
     4681 atagtgcagg agatgaaaga gtttcatcag cccggaaatg gcgaagaaaa gcgaacgcag
     4741 tcccaggatg gcaaagacat tggaggtgta aacgataaag ggatctctgg aaatcgccag
     4801 taccgccgga atggaatcaa ccgcgaacaa aatatcggtg gtttccagcg ccagcaaaac
     4861 ggccagcatg ggcgtggcga aacgctttcc gtttttaatg ataaagaacc tggcttcatg
     4921 aaattctttg gtcaccggaa caacataacg cagcattttt atcgcgatgt ttttgtcagg
     4981 atggacttcc gtttgtttgt tcatcgccag cttgatgccg gtataaatca ggaaagcgcc
     5041 gaaaagatac atgatccagt ggaatttgtt aataagcgcg acacccgcaa aaatgaacgc
     5101 ggcgcgggta atcagcgcca ataggatacc ccagaaaagg gccttgtgtt cataggcttg
     5161 cggcacgttg aaaaaccgga agatcagcag gaacacaaaa aggttatcga tgctcagaga
     5221 gtattcaatg atataagcgg taaaaaattc cagcgccttg gagtggccgt aaaaataata
     5281 aacacccaca ccgaacgcaa cagcaaggga tacccagaag gaaacccaca ggagcgcttc
     5341 cttcattttg atgacgtgag ccttgcggtt aaaaacgccc agatccaacg ccagcatcgc
     5401 aaggataaag acaccgaaga tcgtccacca gagggtcatt gataagtcct tttcctttca
     5461 gatcatccgg cgcaaagcct gagcggcgtt gtgaataata tcccacgccg cgctgaaagg
     5521 cggcgcgtaa gccagatcca gataaccgac ttcgtcaata tttaatccgg tccagagagc
     5581 gacggacagc gcattgatcc gtcccaccgc gccggcaaca cccaccgcct gcgcgcccag
     5641 cagttttccc gaggatttat cggcagtcag cttcagccct atttttttca tgcccattgc
     5701 cggtgccatg gcgctgcccc atgtgatgtt gctgatgggc acaaagcctg cggcaagcgc
     5761 ttctttttcc gagagccccg tggccgcgca ttccagatca aacagccgga aagattgcgc
     5821 gccgacaatg ccgtcgaacg tcaacggatt cccgccgatg ttgcgtccgg ctacacgccc
     5881 ctgtttattg gcgatgtcgc ccagaggaat attgacccag cggcggctga cgcggttcca
     5941 agattcgcag cagtcgccga cggcgtaaat gttttcaacg gatgtctgct gggcgaaatt
     6001 aacggcaatc gcgccggttt tgccgatggt aagaccggca gatttggcca gcgcggcatt
     6061 gggccggacg ccaacggcca gcagcaccat atcgccctgc cactcgcccg cgtcggtaat
     6121 cactttcaaa gaatcgccgg atgatttttc aatggctttt gcctgggcgt tgccgatgaa
     6181 ttcaacgctg tgggactgaa gttcttcgag catcaccgac gatagttctt tactccagcg
     6241 atttgcaggt aaagaatgtt tgtgaatgat ggttgtggaa attcccactt cgtgcagcgc
     6301 ttcgctcatt tccaggccaa tgaaaccgcc gccgataata accacgcgct tggcttttct
     6361 ttcacgcagc caggctttaa tgtgaatggc attttccaga ttgttcagta cataaacacc
     6421 tggcagttcc cggccgggaa tgtccggaac aaaggggttc gtgccggtac cgatgaccag
     6481 ataatcatac ggcagcataa ggttgtccct tgtttcgaca acatttgcat tacaattaat
     6541 attgacgact tccgtgtcaa gctgcacctc cacgccccgt ttacggaatt cttccggggt
     6601 gcgcgccact agtctatttt catctttgat aatgtcgccg atatagtaag gtgtggggca
     6661 ggacgcgtag gcaatgaatt ttcccgattg cagaatcgca acattcagtt ctggatcatt
     6721 tcgtttggct tcagccgccg cggacgctcc gccggctcct ccgccgacaa taattaaatg
     6781 ttttcccata cgacctccaa tgttacctgg ctggtgacgc aatttgtctg cgccataaaa
     6841 caataatgag tccggccgcc atcatcaaca cacaaagaac ctgcccgata ctgaccgaac
     6901 cgaccataaa gtcgggttcg cggaaaaatt caatcatgaa acggacaaaa ccgtagccga
     6961 aaatgtacag ccccagaaga aaaccgtcgg caaacttttt ctttcttaac agccacagaa
     7021 tgacgaacag aaaaattcct tcaaagaagg cttcatagag ctgggacgga tggcgcagat
     7081 gctgcgtcgg atcaaaagga aaatacattc cccagggcat cgttgttacg cgtccccaca
     7141 attcgccgtt aagaaaattg ccgagacgcc cgaacgtgta gcccagcgga atcgaaggaa
     7201 caatcaaatc ggcgaaccgc cagaagttga tctgtcgttt gcgcagaaaa tagatcgaat
     7261 atatcattac gccgaccacg ccgccgtgaa aagacatgcc gctgataccg gtgaaatgaa
     7321 ttccgttgga aaagctgaaa ggcagaaaaa tctccagcgg atgctgagaa aaataggaaa
     7381 aattgtaaat taacacttcg cccagccgcg cgccgacgat catcgcgaac ataccccaga
     7441 tgagatagtc ctgaagcatc tccttcttgt attcgtaatg ttcattgctt atgcgataag
     7501 tggtcagcag ataaacgatg gcgaatccaa ccagatacat caggctgtag tagcgcaatt
     7561 gaaaagaacc gatggtgaaa agagcgggat taatatgcga gggtaattgc tgccaggtat
     7621 tgatgaaagc ttccatgttg tgtttctgtc tgcctaattc atagattttg tcacttcgac
     7681 ccggcgcata ccgaggagaa gccttgtgtt ttagatatct tcccggcaaa gccggactac
     7741 actctgcgtt cgatatggtg acagcttaat atgtctggcc cttgagttca tcaacatata
     7801 gttcggcgct gtaaatcgca ttggcaccgt cgcccgcggc tgtgaccacc tgacggaact
     7861 gtttggcacg gcagtcgccc gcggcaaata tgccgggtgc ggatgtgcgc atgttgtcat
     7921 ccgtgataat gtaaccgcct ttatccagat tcaaaacgtc gcggaagaga gcggtgttgg
     7981 gagtcaatcc gacaaaaata aaaacacctt ccgcttcgat atgattaatt ttgcctgatt
     8041 ccacatcggc caccctgaca ccggtgacaa actcctgacc ggcgatctcg gcaagttttg
     8101 ccttccagat aaattcaatt ttcttttccg caaaagctct tttttgcagg atggccgcgg
     8161 cgcgaaggcg gtcgcggcgg tgaatgacgg aaactttttt ggcaaagtga gtcaggaaca
     8221 gggcttcctg aatggcggta tccccgccgc ccacgaccac gacatccctg ttgcggtaaa
     8281 aaggcccgtc acaggtggca caataggata cgcccttgcc ggcaaattct tcttcgccgg
     8341 gaacgcccaa tttattccac tgcgcgccgg tggcggcaat cacgcttaac gtcctgtagg
     8401 atttatcgcc cacggcaatt tcccagatcg ccgggttgtc gccggctttt ttgatggagt
     8461 gaacatcatt ggcgatgatt tccaggccga aattctgcgc ctgtttcttg aatagctgca
     8521 tcaggtcgaa accgttgatg ccatcaggaa tgccaggata gttttcaatc agatcggtaa
     8581 tggttatctg actgaccgtt gaagcacctt ccaccagcag cgatttcaga ccgctgcgtg
     8641 cggcataaat gcccgcggta taaccgcccg gcccgccgcc gataattatc gtatcgcaat
     8701 cgtattgcgg taaatttgct ttgctcacgt cccaccccct gaataaagtg tctgtacttt
     8761 aatcaagata caggaatttt gtaaagtctt ttaatcacgg gtatgaacca tgatgccgcc
     8821 ccaaggtatg gggtatgata ccctccgctt atagcgggat tgttcgactt acaaatttca
     8881 atacactgcg tttttgaaat ttgagtctta caataaaggg ttatttgtgg taatgatgtt
     8941 ccatcctgaa aggaaaaatc atggagaaac tcaaaggagc gcaaaaaaaa tatctgcgcg
     9001 cacaggcgca tcacttaaag ccggtcgtca tgatcggcgc caaaggctcg acaacgcaac
     9061 tgatcagctc ggttaacgct gctctaaacg accatgaact catcaagata aaattcggcg
     9121 aattcaagga agctaaaaaa gaaatctcgc aggaaatcgc cggtaaaacc aaaagcgaac
     9181 ttgtcggcct gatcggcaac attgccattt tctatcgaca aaacccgaat ccggaaagaa
     9241 gaaaaattaa gctgtcttag caatacaaaa cacaacatcg ctgaaagtgc ccatgtcgtc
     9301 tgaattgctt cgatatgaga tcaaccgaaa aatcaggcag gttctcgtca gccacaatgc
     9361 cgacatgacg aaaatcagct attcctttgt ccatagaacg gtctatatgt ccggcaatct
     9421 cgtccgggag tcccagggcg agttcagcct tcccgttatc gaaggcatga tccgggaact
     9481 catgaaactg ccccgcgtcc aaaaaattct atttgatctg gaaaactgga ttatttccaa
     9541 tgagccggga gcattaaaca ttgtcaagaa aaaaggattg ggacaacatc ccgcgataaa
     9601 agactcggtt taacttggaa ataaagctgg atctacccag aggtcaccat agcccgacaa
     9661 gttcattcgg ggatacgaaa agacagatgc tatcatgccg ttgcaaaacg gcatccagaa
     9721 aaaaactgga ttccggccta cgacggaatg acaaagaaaa ggggcaatcc tttaccttgc
     9781 tcatgtcaga tttactgagg ataaaacaca gcggcaaaga ttcatatgcc ggtcaatatt
     9841 tcctgtttga agtaagcaat caatataatg ttttcgctat cgtttatgcg attccggcca
     9901 gtcgattttg tgcagtgtca gatagcgttc cacagcttgt tcaatggtcg gaaagaaatt
     9961 ctcctcgccg aatcgtgtaa aaagtccgta gcgcttgagc atgtccttga ccgggtcttt
    10021 catttcggca aaaaataatg ccatgcccga ttcgtgcaga acattatcca gttcggccag
    10081 catgtctgcg gcagtggtgt ccacatcggt aatcggttcg gccgttacaa caacccactt
    10141 ggtgggtgtc ggcgcttcgg caatggcatg aagcacattc tcgcggaagg cttcggcatt
    10201 ggcgaaaaac agcggcgcgt cccagcgaaa aaggaccagg ccgggaatca atctggcatc
    10261 gggatgacgg gatatgtcgt gataactttt taaaccttcg acatgtccca atacggcatc
    10321 gtaagggtgc caggcccgcc agatgaagga aaacagcgcc aggccgatgg cgataaaaat
    10381 acccttgatg atccccaggg aaaccacgcc cagaaaacac gcgatagaga aaacgaattc
    10441 ttcccgccgc aatttataca aacgcgccag ggcgcgaact tcaacgatgc tgatgcaagc
    10501 gcagatgacg acggcgccca gaaccgcctg gggcagattc ttgagcagat tcggcgcgaa
    10561 aatcagaagc agggcaatgc ataccgcgcc gactactccg gtcacttgag ttttggctcc
    10621 cgccgattcg gccaccggtg tgcgtgaggc gctgctggtc acgggaaatc cctgaaataa
    10681 tccggcggcc atattggctg cgcccagcgc gatgatttcc tgattgctgt ccacttcctg
    10741 accggcacgc agtgcaaagg ttcgcgagag tgtgctcata tcggcaaagg aaaccagcgc
    10801 gatggcgaac gcggcagcaa ataacgcttt aaattcagca aagctcaccg agggaatctg
    10861 gaattgcggc agtccctgcg gcagcggccc gacgacggca atgccgtaac ttgcggataa
    10921 atgaaaccag gaagagatga tcgttgcgcc ggccacggcg atgaaaaccc ccggaatgcg
    10981 ggagaaccat tgccggcaac ccagaataat catcaggcag gaacatccga tgaggcacga
    11041 catccagttg atctgaccgc cggcgatgcc ctgtatcagg ctgttaactt cctgcaaaat
    11101 attattgccg ccgacgggaa aacccaaaac tttcggcaac tggccgatca gaaccgtaag
    11161 cgcgattccg ttgaggtatc cgatgcggac cggtttggaa agcagatcag taatgaaacc
    11221 gaacttcaca agaccggcca gaatgcaaaa agctcccgcg aggattgcca agaggccggc
    11281 aagcgcgatg gccttatcgg tattgccggc ggccagcggc aggatggccg ccgcgatgag
    11341 gccggccagc gccgagtcgg gtcccagaat cagaatccgg ctgggtccga aaatcgcgta
    11401 agcaatcagc ggaatgatcg tggcatacaa tccgtatatc gccggcaatc ccgagaccga
    11461 tgcgtaagcc atgccgaccg ggaccaggat ggccgtaagc accagaccgg caaggatgtc
    11521 gtgcatcagc cattcacgcc tgtagtttcg caatgtttcg atcaggggaa tccgcctgga
    11581 taaaccgcgc ggacgcagat gatgaacaaa cggcttgacc gtgggaattt ttgtattatt
    11641 caaaaacaga cctcatgtat ttcgtcgctt gattatctag ccgatgtgtt ttaaaaaata
    11701 aagaattatt caaagcggat gagcaacgac attctcagat gaaggagata tgacatgccg
    11761 tttaaaacac tgataaccgt cctgatctgc aattttattt tcgtgatgat tgccgttcct
    11821 ctggcgctac gcaaagtgcc gaaaaatgtg atttacggtt ttcgcattaa agccacgctg
    11881 gaaaatgatt ttgtctggta tgaagccaac gcttatttcg gaaaggtatt tatcatcggc
    11941 aatatggttt gcgcgctgtt gatcctgatt ctctattttt ctgatgttgt tcccgcacag
    12001 aactttgtca atatcggcat ggcgattctg attattcctg ctctggtggc ggcattattg
    12061 acgtttcgca ccatcaaaac aaaacactaa atggcaggta tttcataaag caggcggaag
    12121 gtttctgaag ggatcccgcg tcagttttta cgaaacttgc cctgcggaga cgaggacgcc
    12181 agggaagcat acaagagcgc cggctggagg ctgtgatttt tccgttgcgt gttattcgta
    12241 tcgaagaaga gggcgacggt ataaatatta ttcatggttt gagttgtgtt tttgaattcg
    12301 gccatttgtg ccgctgggac aggactgccg gcaacttttt taaccgccag cggattaatc
    12361 gccctgcctc cctgtctcat ttcaaaatgc aggtgcggtc ccgtggccaa accggtggct
    12421 cccacatttc cgatcaggtc accctgctga acttttttcc ctgggcgaat attgcttttg
    12481 atgcgggaca aatgaccgta atacgtctga tagccgttgg cgtggttaac gataacgatt
    12541 ttaccgtaac cggatttata acccgcaaac gtgatccgtc cgtccgccac cgtgctgacc
    12601 ggcgttcccg tggcggcaac atagtcaatg ccgtgatggg gacggcgcac cttcaggatg
    12661 ggatgcaggc gacttctgct gaaatgagaa ctgattctcc ggaaactcag cggcgccttt
    12721 aaaaaggcct tgcgcagcga ttgcccgtcg gcattgtaat aatccgtctt gccgtcctgt
    12781 tcgaaaagat acgcgttgta ggtctggctg ccgttgacaa attcgatgga cagaattctg
    12841 ccgtattttt tgaatttccc ttccgaatac aggccttcaa caatgattct gaatgtatct
    12901 cctttgcgca catcggtgtt gaaatcgata tcccaggcca gaatgtccgc cgcctgcagg
    12961 gccagcaggt aatcttcccg ctcgttgccg aacgcggaaa ccagcgtgtt ttgaatcgtg
    13021 ccgccgatgg tcagaatacg ggtctcataa ggaatgttat atttttgcgc ctggaaacat
    13081 ccgtccaccc gttcaatttt taaaatggtg tcgtcatcga tgccgcacgt aaaagagctg
    13141 atgtaattat cttcattgag cgtaaaactg taaggcttgc cgggcttaat atcccgcagc
    13201 gactgaatgc tggccaccgc acttttcagt tcgaaaagat catcggtgtg catcccgtat
    13261 tttttaaaaa tcgaggaaag tgtttctccc ttttcgaatt tgtcgcttat ggattttaac
    13321 ggcctggttc ccttttccgg agcggttggt tccgatacaa cggcatcccc ccgaaaagaa
    13381 ttctccgaag gctgctgcaa agaaaataac ccgatcgtaa taaagagaat aaccagtaaa
    13441 actaaacgct ttttattcaa taagttcact cctgaccttt gaacaattgt ttgtgctatt
    13501 gacttattat gacgattatg tcaagaaata atttccatgt acttgaaatg attgggtatt
    13561 gtattattga ctgcaggcgg ctgcaacgcg aatttaataa gattcagata tttcaagtta
    13621 agtgccgcaa gatctgtaaa tcgggttttt ccgtctgtga agtcaggtga aacttgtaat
    13681 aaaatcaaag cctcagaaga aataaccgga agccggaaga ttgctacagg ataaccatcg
    13741 gggcatctgc aacggattga tgcaaatcga atggtccttc ccgcttacct tctggtaagc
    13801 tggcggtact tgatacgatg gggctgactg gcggcattgc cgtatcgctt cctgtaatct
    13861 tcctcgtatt cggaatagtt gccgtcgaag aacagcacct tgctgtcgcc ttcaaacgcc
    13921 agaatgtgcg tgcagattct gtccagaaac cagcggtcgt ggctgatgac gacgacgcat
    13981 ccggcaaagt tttccagcgc gtcttccagc gcgcgcaagg tgttgacatc cagatcgttg
    14041 gtcggttcgt ccaataacag gacattgccg ccttccttga gcatgcgggc caggtgcacg
    14101 cggttgcgct cgccgccgga gagcgttccc acttttttct gctgatccgt tccggagaaa
    14161 ttaaagcgtg aaacataggc gcgggaattg atctgacgat taccgatagc gataatatcc
    14221 tgtccgtcgg aaatcatttc ccagatattt ttattgggat ctaaggaatc gcggctctgg
    14281 tcgacataag ccagtttcac cgtatcgccg atgcgaatcg tgccggcatc cggcttttcc
    14341 cggccggtga tcattctgaa aagagttgtt ttgcctgccc cgttggggcc aatgacaccg
    14401 ataatgcctc cgggcggcaa tgaaaacgtc atgccctcca ccagcagttt atcgccgtag
    14461 cccttgttca catccttggc ttcaatcacc agatctccca gacgcggtcc gggcgcgatg
    14521 aagatttcgc gcgtgccgga ttctttttcc atggttttgc ccagcagttc ttcgtaagcg
    14581 ctgatacgag ccttggattt ggcgtgtctt ccttttggcg acatgcgaat ccattccagt
    14641 tcgcgctcga gagttttgat tctgtcactt tcagcctttt cttcattttt cagacggttc
    14701 tgtttctgtt ccagccagga ggaataattg ccctgccagg ggatgccctg accgcggtca
    14761 agttccagaa tccagccggc cacattatcc agaaaataac ggtcgtgggt aacggcaatg
    14821 atcgtgcctt cgtatttttg cagatgatgt tccagccagg cgacggattc ggcatccaga
    14881 tgattggtcg gttcatccaa gagcagaatg tccggtttct gcaaaagcag gcggcacagc
    14941 gcgacgcggc gtttttcacc gccggagagc accttcacga atgtgtcgcc gggcgggcag
    15001 cgcagggcat ccatcgccat ttccaggcgg gaatcgatat cccaggcatc cagggcatcg
    15061 agtttttcct gcacggcgcc ctgtctttcg caaagtttgg ccatctcgtc atcggacatc
    15121 ggttcggcaa atttttcgtt gatgtcgttg tattcctgaa tcagatccat cgtactttgc
    15181 acgccctgtt ccacaatctg ccgcacggtt ttatcgttgt ccagatgcgg ttcctgctcc
    15241 agataaccca ccgtgtagcc gggtgaaaga atggtttttc ccaaaaactc ctggtccaca
    15301 ccggccagaa ttttcagaag agagcttttc cccgagccgt tcaaaccgat gacgccgatc
    15361 ttcgccccgt aaaagtaaga caggtagatg tccttgagca ccggtttttt atcgtagatc
    15421 ttgcttaccc cgatcatgga gtaaatgact ttgtttcctt cgttggccat aaaaaggtcg
    15481 ttctccgtga tagaggattt atatgtaagg gaagataccc ttgataatca gattgaaatt
    15541 gttctgaatg ctgcttttcc cgtgcagaat gcccatgtgg ccgggcagga gccattccag
    15601 atccagcttg gcgaaggcga gaatgctttt tttcaaaaga tcgccgttgc cgccgggaaa
    15661 atccgtgcgc cccacgcttt gatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnngtaaaa actgttgcag ttgttttcat aaggattggt ccactcgtaa agataaatat
    15841 cgttttttaa tctcatgctt actcctgtat cgttgatttg tcgggaacaa aaatccacag
    15901 caggatatac aatagaaccc ctgtgccgaa acataacaca agcgtcacaa acaacagacg
    15961 ccacgcccac gccggaacgg gagaactttc ccccagaccg ccgcacaccc cgccgatcca
    16021 tttatcggtg tttgatcttg tgagattctg cagccagttg cgctcgttca ttttcattcc
    16081 ccccggaatt ctgtttattt ttttaccgaa aaaagcctgt ccaccggcaa gtcagatatt
    16141 cggtgaccga catcgacgat attgccctct ctgtccagaa gaatcagggt cggaacgccg
    16201 ataatattat aatcgccggc tacactgccg tctccgtcca ccagcacgtg gtacggaaaa
    16261 gcattttgtc cggcaaaccg tgccacccgt tcggcggatt cgttgatatc aatatacagg
    16321 aactttaatc cgcgtccggc atatttattg taaaggttcc tgtaaagcgg catttcctcg
    16381 cggcaggcgg gacaccaagt cgtgccgaaa aacataatga ccggatttcc ccgctgggcg
    16441 ctcaaccgga atgttttccc atggatgtct ttcaatgaaa aatcaggagc cgaaactttg
    16501 tcgcgtcttg taaaaacact cggcttaaac tgttcaaaag agaaagcaac caacgcaaaa
    16561 acaaacacca gagcgattgc cagtaaaatt tttttgttca tcatcacctc tttttatata
    16621 gacagtcttc cggcaatata caggaaatat tccgcagcgc caattaatat cagcccggat
    16681 atgaatttaa ttttttccat ccatgctccc gatcgcggca aagcggcaat catcccggca
    16741 aacgtgccgg cgataatcag cagcgttccc atcccgaaag cgaaaataaa cagcaggctc
    16801 atccccagaa ccacatttgt tttgaccgct acgtaaccga gtaaaacccc gagggccggg
    16861 gcggtgcagg gtccgatgac cacgccggaa acggcgccga ttaaaaaact gttcagaaat
    16921 ccttttttgc cgtttgcacc ggtccattgc atcagctttt gcggaacagg gatggagagg
    16981 ttgaacacgt ccagcatgga caatcccatg accagacata aattggccat gataaaatac
    17041 gtccagggcg tcgtctgcat ctgaccgaat atctttcccg acagcgccgc cgcagcaccg
    17101 agaaccgcat aggtaaccgc cattccggcc acataaaaca acgagaggaa aaaaccgcgg
    17161 attttggatg acggtccctg gatgccgatc aaccccacgg ttaccggaat cagcggatac
    17221 gtgcagggcg taaaactgat aatcacgccc cccagataag cggcaaggac agataacatc
    17281 agcgagcttt caagataaaa tgacagatta ttaacaaggg gttcaatcat caaatactgc
    17341 ctgtgtctgg ttgttcgtta ccggataaaa aagtttaaaa aatcaacaga aaaagatcgt
    17401 tttcggctgt gacaagttat gattatgcaa atcaataatg ataaaaaatg gtgccgaaga
    17461 ctggatttga accagcacag gcgcacgcct actagaccct gaacctagcg cgtctaccaa
    17521 ttccgccact tcggcacaca aaaagtgcac tataccgatc ggcacgttct tgtcaagata
    17581 aaatgagctt gaatttaatg tccaacatgt tatgttatca agctactggt tgacgtttat
    17641 agtatgaaac aatgattata ttcaggagta cggaaaacat aaccggagat tttcgcggat
    17701 catttgtctc cataggcaat ttcgacggcg tgcatttaag ccatcagttt atctgccgga
    17761 aactcgcctc cgaagcgaaa caggccggga ccaaatcact ggttattacg tttgatccgc
    17821 atcccaagat gattcttcat ccgaatatca ggccgttttt tctgatcacg acgcttgacg
    17881 aaaaactgga gcggcttgcg gcttgcggaa tcgacggcgt tctggtcatt ccttttacca
    17941 tggagtattc caaaacgacg gcggaagaat tcgttcataa ttttctgggg cggaaactgg
    18001 ccatcggaaa aattattatc ggacatgatt acaccttcgg tcaggggaaa aaaggcaaca
    18061 gtgattatct gattgctgcg ggacgggaca tggggtttgc ggttgaagtc gttgacgccg
    18121 ttaaatcggg aaacgatatc atcagcagca cgctgatcag gaaatccatt atcgaaggca
    18181 atctgaaaaa agccgccgtc ttgctgggac gatactacaa tgtcgcagga aaggtggtca
    18241 ccggtttcgg acggggcgtc acgctcggat ttccgacggc caacatcgaa ccggaaaaag
    18301 aattgctgcc gccgccgggc atttatgcgg cgtttgttga tgcggaagaa aaacggtacc
    18361 aagccgtttt aaatatcgga gcgaaaccga cgtttgaaga ttataccttt tcctttgaag
    18421 tacacctgct tgatttttcg ggtgatctga ggggaaaaag gatcaatacc gattttgtgg
    18481 agaagctgag ggacattgtc aaatttgact ctcccgaaac gttgaagaaa cagattgccg
    18541 cagatgtgga aaaagcgaaa acgattttgc ttgaaaatat aagcaaccgt caaggaactt
    18601 agaggaggtt tttgaattat gttaaaaatt gctattgtcg gcgcaaccgg aatcgtcggc
    18661 cagcaggcga ttgtcagtct gctcggacat ccctggttta aaatcgccaa actggtggct
    18721 tcggagcgat cggccggcaa gaaatatatt gatgcgttac gggacgccaa cggttcgatc
    18781 cgctggtggt gcccggagga attgccgggg catattgcca atatggtggt ggaagaagca
    18841 gcccgtttcg atccgaaatc cgtggatatt attttctcaa ccatggacgc gggaccggcc
    18901 agggatctgg aacccaagta cgccaaaacc actccggtga tcagcaccgc gtcggcattc
    18961 cggtatgaac cggatacccc cattctggtg ggcggtgtga atatggatca cgcggccctg
    19021 ctggccgttc agcagaaaaa ccgcggctgg aaaggtttca tttcacccaa atccaattgc
    19081 accattgtcg gactggttgt ttcgttgaag ccgattctgg ataacttcgg cgtaaaacaa
    19141 gtggtggcga cttcgctgca ggccatgtcc ggcgcgggac gaacacccgg cctgtcggcg
    19201 atggatatga ccgaaaacgt cgtgccttat attcccggcg aggaacccaa ggtggaaacc
    19261 gaaccgctaa aaattctggg caagctgacc ggaaaaatca tcgtcaacgc cgcgttcggc
    19321 attaccgcaa cctgtacccg cgtaccggta ctggacggcc atatggtttg cgcgtttgtt
    19381 caaaccaaga aaaaatgctc gccggaagac gtcaagaagg ccatgatgaa ttacggcaag
    19441 gactttgccg aactgcatct gcccagttca ccggcgcatc tgatcgatgt gaccgatgat
    19501 ccgttccgtc cgcaaccgcg gatggataga gacaagggcg gcggcatgac cgtaaccgtc
    19561 ggacgcatcc gcaaggatcc ggttattgaa aacggcatca aatacgtctg tctggcgcac
    19621 aataccaaaa tcggcgcggc caaaggcgcc ctgcagacag cggaatacct ggtcaagaat
    19681 tatctgaaat gggtcaaata aaatttaatg agacccaaat ttcagaaacg tagtgagact
    19741 tgctaaaatc gagcgaagcg aagattgaag cataagtcga attatcccgc aaagcggtgg
    19801 aagtgcactg aaatttgatg gtcgaattaa tgaaactcaa gtttaagttt gctacgcttc
    19861 tgaaacttgt aagttgaatt atcccgccgt aggcggagga tatcgcagat atccttgata
    19921 tccccgaggc gaataaccta aaggtcacta taggccgagc gggataaatg cgggtctaat
    19981 gccccgcccc ttggggcggg gttgttcatt ggactgcggc atgatttaaa aatgccgcgg
    20041 tccgttgttt aaaaatgcat ccgtcaaagc acgttgcatg cagcctcgga aaaatcagtt
    20101 tttcaaacac gcttcgtaaa tctcaagatc tttcccggaa aaacagcaga aaatcacttc
    20161 ttctatcgtt gataatttct gaaccgtatc gcgcacggtt tttaccgcca cctgcgccgc
    20221 aggctcaacc ggatatccgt aaacaccggt gctgatactc gggaaggcga cggttttgat
    20281 gccgttttcg gcggccacaa tcagagaact gcggtaacag gacgccagca gttgggattc
    20341 accgctttta ccgccgtgcc agaccggtcc tacggtatgg atgacaaaat ttgctggaag
    20401 attgtatcct ttggtaagtt tggcctcgcc ggttttacaa ccgccgagtg tccggcattc
    20461 cgccagaagc tccggacccg cggcccggtg aattgctccg tccacacctc cgccgcccag
    20521 aagcgattga ttggccgcat tgacgatggc atcgacctta agggttacga tgtttgcctg
    20581 aaccgctttc aattgagtca tcgaaccgcc tccaaggcat ggagatagca ccgctgcccg
    20641 aatcagcggg cagtggtgct cgatttagaa gttttacttc atggcctgtt ctaccgccac
    20701 cgccacggcg acggatgcgc ccaccatcgg gttgttgccc atgccgatca ggcccatcat
    20761 ttccacatgc gcaggaacgg acgacgatcc ggcaaactgc gcatccgaat gcattctgcc
    20821 catggtgtcg gtcatgc
//
LOCUS       DAOSME010000041        20563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3202_length_20563_cov_21.219670, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000041 DAOSME010000000
VERSION     DAOSME010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 20563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20563
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3202_length_20563_cov_21.219670"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..312)
                     /locus_tag="PLG98_07355"
     tRNA            complement(237..312)
                     /locus_tag="PLG98_07355"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Ile,seq:cat)
     gene            complement(537..2117)
                     /gene="rpoD"
                     /locus_tag="PLG98_07360"
                     /pseudo
     CDS             complement(537..2117)
                     /gene="rpoD"
                     /locus_tag="PLG98_07360"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(2223..3995)
                     /gene="dnaG"
                     /locus_tag="PLG98_07365"
     CDS             complement(2223..3995)
                     /gene="dnaG"
                     /locus_tag="PLG98_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOE33109.1"
                     /translation="MYVDQSKIEEIKNRLDIVDLVSEYLTLKKAGRNFIGLCPFHQEK
                     TPSFTVNRDKQIFYCFGCGEGGNVITFLMKIADKTFPEAIRDLAVKTGVVLPPVSSGK
                     EARQKDLLKENIVSLNSKAAQQFSRNLFSASGTPARQYLQQRGIAEDVVKQFRLGYAP
                     DTWRSLTDFMEKSGLSLKLAEQAGLIIPGKEGGFYDRFRGRLIFPIENVFGEIVAFGG
                     RILGKGEPKYLNSPESPVYIKGKNLYGLNKAKDDIRKKDFALIVEGYFDLISLWNAGI
                     RNVVATLGTALTRDHLDLLRRYCVDVVALFDPDEAGKKALDRSLELFLSTEMRARALI
                     LPDGYDPDDYVRKFGQDKMEDLIALAQPLSDYYIENVIGGGRTFEDKRDMVKTAMTFL
                     SKIGDQKEKNLFIKRIAEKTGLNEELLIREAYSRVYSGRTETNRNNPAVNVHHDTVEL
                     HLIQLMMEYPQKIVQVENENVLDYFLQPDIRKLGEKIIDAYKLLGFVDVGVILSTEEN
                     QALRERIFQLMIKALPAEDDVLGKILTDNIRQIKKRWFKEQHRQIKIKLSRAQDSGNE
                     EMVHQLLYEKEQMLIKEKELQSTG"
     gene            complement(4043..4504)
                     /locus_tag="PLG98_07370"
     CDS             complement(4043..4504)
                     /locus_tag="PLG98_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOE33110.1"
                     /translation="MEGEKMDINAKLSEEMVAAAKAKDKIRLSAARMLKTALHNKEID
                     LMRPLHESEMLQVLSSMIKQRKDSIEQFAKGGRTDLVEKEEAELKFLQEFMPAQMSDE
                     EVDALIKKTIEDAGAVSVKDMGKVMKILMPKLTGVADGKAVGEKVKAFLSQ"
     gene            complement(4508..4852)
                     /locus_tag="PLG98_07375"
     CDS             complement(4508..4852)
                     /locus_tag="PLG98_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOE33111.1"
                     /translation="MSDCVFCKIVRGEIPCARVFEDEHVLAFDDIHPMAPVHVVIIPK
                     KHIATLMDVDVDNSDVIHRLTAAAQKVARIKKIDQSGFRTAINCNAEGGQVVFHLHVH
                     VLGGRKLQDELG"
     gene            complement(4871..5608)
                     /gene="hisA"
                     /locus_tag="PLG98_07380"
     CDS             complement(4871..5608)
                     /gene="hisA"
                     /locus_tag="PLG98_07380"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOE33112.1"
                     /translation="MIIIPAIDIKDGKCVRLAQGKFDRVTTYADNPLDMALLWARKGA
                     ELIHIVDLDGSVAGRPRNADIILNIVENINVPVQIGGGIRDMETIRFYLDQGVSSVIL
                     GTTALKNQQIVREACTVFPGRIILGIDALNGKVAVRGWTENTEKNAVDLARQYEPYGV
                     KAIVYTDIERDGMGTGINMKATRELADSVSIPVIASGGVASIADVDNLMVVEGGSIFG
                     VIIGRALYTGAVDLEEAISKTKKVTRT"
     gene            complement(5761..6447)
                     /locus_tag="PLG98_07385"
     CDS             complement(5761..6447)
                     /locus_tag="PLG98_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33113.1"
                     /translation="MVAILRKYFCFIVRFVFVALLAFPVMSQALDKKAQSDESENLIA
                     VIAFQPLRPEGGTGNTVLCPICGKASAGGIIQEGAEKIVEEIFVEQLKARDDIVLVPA
                     EKLQKATQKYSTDSMKDFQATGLLKIGRELKADFLVVGYVYRFAERVGYRYSSEHPAS
                     VVFEIHLIRTADGKSVWRGYFDKTQRSLMENVLDISSFFKGGAKWMTARELTELGMEN
                     VFQTFPDFQP"
     gene            complement(6554..7141)
                     /gene="hisB"
                     /locus_tag="PLG98_07390"
     CDS             complement(6554..7141)
                     /gene="hisB"
                     /locus_tag="PLG98_07390"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOE33114.1"
                     /translation="MARKAKIVRKTTETDIRLEIDLDKTAGSSIETTIPFFNHMLELF
                     ARHGFLKLIVRSKGDTHIDDHHLVEDLGICLGQAVAKALGDKKNINRYGSARVPMDEC
                     LCQVDMDICGRPYLIYHVKSGRRKIGKFDPALVKEFFKAFTDQSGITLHINFLYGENI
                     HHMIESIFKAFGRALRNAVSLNENIKGVLSTKGKL"
     gene            complement(7156..8028)
                     /gene="hisG"
                     /locus_tag="PLG98_07395"
     CDS             complement(7156..8028)
                     /gene="hisG"
                     /locus_tag="PLG98_07395"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOE33115.1"
                     /translation="MKNLKLGIPKGSLEANTIDLFKKAGWRITSDARSYFPDIDDPEI
                     SCKLVRAQEMSRYVEDGTLDLGLTGIDWIMENDSDVVVVQDLIYSKVSTQQARWVLVV
                     RDDSPYKKIEDMEGKRISTELVNFTKKYFAERNINIHVEFSWGATEAKVVEGLVDAVV
                     EVTETGSTIKANKLRIIHELMKTNTQLIANRAAWKNAWKRKKIEQISTMLTGSLQAMG
                     KVGLKLNVSKENLKGVIGLIPSLKSPTISGLSSDKWFAIESIVDAKVMRDLIPKLLDA
                     GAQGIIEYPLNKVI"
     gene            complement(8025..8399)
                     /gene="hisI"
                     /locus_tag="PLG98_07400"
     CDS             complement(8025..8399)
                     /gene="hisI"
                     /locus_tag="PLG98_07400"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOE33116.1"
                     /translation="MIEPDFAKGDGLIPAIVQDAETKDVLMMAYMNRESWDATLKTGK
                     ATYWSRSRKKLWLKGESSGNVQIVKAVFIDCDEDTILLHVQQIGDAACHTGHRSCFYR
                     KLEGNDFVAVGEKIFNPEDVYK"
     gene            8563..8856
                     /locus_tag="PLG98_07405"
     CDS             8563..8856
                     /locus_tag="PLG98_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33117.1"
                     /translation="MSLLIGGIISAIFGLVSLIFFWPDFMTIFKGSLPIFLLLGGALA
                     VYVGIDEIQEKNRQERHLQEEKLEHAQKEIETIRAQAESYREELQKLKDSKQP"
     gene            complement(8935..9423)
                     /gene="lspA"
                     /locus_tag="PLG98_07410"
     CDS             complement(8935..9423)
                     /gene="lspA"
                     /locus_tag="PLG98_07410"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOE33118.1"
                     /translation="MKKNIIIFVLGAAVIVVLDQITKAAIVKKFFLHETRSVIDGFFN
                     LVYVMNPGAAFGFLAGMSEMFRSLFFIGMTLLVMALIIYYIAKSKSENMFFVVSLTMI
                     FAGAVGNLIDRIRFGAVVDFLDFYIKTAHWPAFNVADSAITIGAVLMIWGMVAGRKRE
                     AV"
     gene            complement(9439..12228)
                     /gene="ileS"
                     /locus_tag="PLG98_07415"
     CDS             complement(9439..12228)
                     /gene="ileS"
                     /locus_tag="PLG98_07415"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOE33119.1"
                     /translation="MDYKKTLNLPQTDFPMKANLAQREPEMLKKWEDMNIYKKIRQMA
                     KGKTPYILHDGPPYANGNIHLGTALNKIIKDIVIKSKNMTGFDGVYVPGWDCHGLPIE
                     HQVDKELGAKKGAMSQVEKRRACRTYAEKYVDIQREQFKRFGVFGEWENPYLTMAYPY
                     EAVTVEEFGKLYLNGSVYKGKKPVYWCATCRTALAEAEVEYADHHTPSIYVKFNFVSD
                     IAQVLPKLAAQKVAMVIWTTTPWTIPANLAIAVKDDFIYVAVKINDDVMIVAKDMLDY
                     CLDAFGYQGKSYEIIDEFKGAVLEGQKCVHPLIKRNSLLILAPFVTLDAGTGAVHIAP
                     GHGQEDYEIGLEYGLDNYAPVDDAGKFTEDVEHFAGQFVFDANDSVIKKLDEVGALLG
                     HVPMQHSYPHCWRCKKPIIFRSTEQWFISMEKNDLRKKALECINEVTWIPSWGKDRIY
                     GMVENRPDWCISRQRLWGVPITVFYCAKCKNEVMTREMMDHLVALVEKNGADVWFEKD
                     PKDLMPKHTTCPHCKGTEFTKEVNILDVWFDSGVSHAAVLEKRPDLSSPCDMYLEGND
                     QHRGWFHSSLLESVGTRGRAPYKNVLTHGFVVDGEGKKMSKSVGNVIGAEEAINKYGA
                     EILRLWVAAEDYTDDIRISEEILKRLMEAYRRIRNTSRFILGNLYDFNMDKDAVAYEQ
                     MSELDRWALHRLQEVIKRVTEAYERYQFHVVFYTLYNYCTVDLSALYLDVLKDRLYTN
                     KAASAVRRSGQTAMFIILNAMTRLLAPILTFTSEEVWAAMPDWTNKEDSVHLAQFPEV
                     SGTFFNADLGERWKAMIDAKSEIAKAVEQARKEKIVGHSLDARITIAAPEKLRALFAA
                     HLEDLKALLIVSQLQLADEQDIAAPFRSAEIEGLMVGVEKARGAKCERCWIYDESVGA
                     DAQHPTICARCLPNL"
     gene            complement(12475..14310)
                     /locus_tag="PLG98_07420"
     CDS             complement(12475..14310)
                     /locus_tag="PLG98_07420"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HOE33120.1"
                     /translation="MKKNDVVQYPQTISGFEFFVKFMAFVGRIPFVVYHGIKGIKSIS
                     SNRNASWGSLLQKSALKYPNNPAVKSPDGTLTYAELNEKANQFAHFLLSKNVKKGDTV
                     AVCLENRPELLFLYCGCAKVGAVCSLINTNQRGDSLVHSFNLNKGKVMVVGEECYSFF
                     KDVKDRIDLQGSELCLLRDPAMNQLKAPEDITDQLAVLPKTNLSETGGISLKDTMAYV
                     YTSGTTGGLPKAAVITHKRAMSATFWFGRVVTHVKPKHTIYIPLPFFHTNAITVGWPP
                     ALYSGAAVAMRRKFSASGFLDDVKKFQATHFVYVGEVCRYLMAQPEKAGENKTTLKYI
                     IGNGLRPDIWMAFKRRFGISKIYEFYGAAEGVGVFTNLMNFDYTVGTSLTPFAIVQYD
                     IENDCPARDAGGFLIKVAKGDPGLLIMEISEKTPFPGYTDKKKTEEKIIRNAFRKGDL
                     WFNTGDMLRNMGFRHAQFVDRLGDTFRWKGENVATMEVEKALDSFSGVSASAVYGVVM
                     PKGDGRAGMAAVIKQEGKDVDFAALAEHLRSVLPKYAVPIFIRFVNDFQWTATHKIKK
                     NDLKNDGYNLSAIKDGLYVLLPGTETYVPLNDAIYQDIMAGKYKF"
     gene            14636..15529
                     /gene="htpX"
                     /locus_tag="PLG98_07425"
     CDS             14636..15529
                     /gene="htpX"
                     /locus_tag="PLG98_07425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOE33121.1"
                     /translation="MKRILLFLATNIAVLVVLSIVLRLLGVDRILDESGMNLNMVNLL
                     VFAAVFGFGGAFISLAMSKWMAKRMMGAQVITNPQSATETWLVDTVKKHAQMANIGMP
                     EVAIFNSPSPNAFATGMNKNKALVAVSAGLLHAMNKEEVEAVIGHEISHVANGDMITL
                     SLIQGVVNTFVIFLSRIVGHFVDRVILKNEEEGHGIGFFVTSLVAQIVFGILASVIVM
                     WFSRKREFIADAGGAKLAGTQNMINALEALKRGTAEPLPDQMAAFGINGKPSGHGLKL
                     LFMSHPPLEDRIEALKKLTYR"
     gene            15857..16909
                     /locus_tag="PLG98_07430"
     CDS             15857..16909
                     /locus_tag="PLG98_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33122.1"
                     /translation="MKKIVVCVVVMMFLVTLPFAALAKKVSLTEEELSSTTAQEGVTI
                     QFGKNTYGGDYFFDTNFYVAGKFLPTIQSWGDGDGCTDCGGYTEKGWIGVRNVSMDPF
                     VSLGLPNPAFPPTGSFISLYRTMKIDVGTSGGATKLFIGMPSIMVHPSGISQTLSSGT
                     TETLGQDFGTAYMSEFALIANSAGTGYLRIGNHSSTGQQGVEIQFSSTSPVAAGPLYG
                     VVFSIPGKPIVQSWSDIDGEQTGSGYTNAGYFGAKNFTMSDGTTYAWPGSPSTFNMIV
                     TGTLSIDVGTNGSGQTAVVLGVPTVFFAPNAAITSPFGFATDRELNTNWQSLGTLYTA
                     GIDIHQMRGSLAISAH"
     gene            complement(17631..19274)
                     /gene="groL"
                     /locus_tag="PLG98_07435"
     CDS             complement(17631..19274)
                     /gene="groL"
                     /locus_tag="PLG98_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOE33123.1"
                     /translation="MGAKILLYDEEARKSVLKGVNTLADAVKVTLGPKGRNVIIDKSY
                     GSPTVTKDGVTVAKEVELEDKFENMGAQMVKEVASKTSDVAGDGTTTATILAQAIYRE
                     GVKAVAAGSNPMDIKRGIDKAVETVVKELSKMSKPTKDQKEIAQVGTISANNDETIGN
                     IIAGAMDKVGKEGVITVEEAKGLETELEIVEGMQFDRGYLSPYFVTNADKMLVALEDV
                     LILIYEKKISGMKDLLPILEQIAKMGRPLLIIAEDVEGEALATLVVNKIRGTLHVAAV
                     KAPGFGDRRKAMLEDIAILTGGKMISEDMGYKLENVKLEDLGRAKKITIDKDNTTIID
                     GAGKRAELEGRVKQIRAQIEETTSDYDREKLQERLAKLVGGVAVIKVGAATESEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVAYLRTLPALSKMDLTGDEQIGVNIVKKAIEEPI
                     KMIACNAGLEGSIVVEKVKEKKGAFGFNARTDEYEDMISAGVIDPTKVTRFALQNAAS
                     VSGLLLTTQCMIADKPEEKGAGGGMPGMPPGGGYPGMGM"
     gene            complement(19301..19591)
                     /gene="groES"
                     /locus_tag="PLG98_07440"
     CDS             complement(19301..19591)
                     /gene="groES"
                     /locus_tag="PLG98_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOE33124.1"
                     /translation="MKIRPLQDRIIVKRLEEEQKTKGGIIIPDTAKEKPIEGKVIAVG
                     KGKVADDGKLIKPEVSVGDKVLFSKYGGTEVKIDGEEYLIMREDDILGIIEK"
     gene            complement(19701..>20563)
                     /gene="radA"
                     /locus_tag="PLG98_07445"
     CDS             complement(19701..>20563)
                     /gene="radA"
                     /locus_tag="PLG98_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOE33125.1"
                     /translation="VVIDSIQTVYASELMSAPGSVGQVRETSSRLILFSKKHGIPVFL
                     VGHVTKDGSIAGPKVLEHMVDTVLYFEGDSSHSYRIVRGMKNRFGPTNEIGVFEMREK
                     GLIEVPNPSTFFLAERPQNVAGSVVVPSLEGTRPILVEIQALVSSTSFGTPRRTAIGV
                     DYNRVSLLVAVLDKICGLHLGGSDIFINVAGGVKVEEPAVDLGIVAAMASSFLDKPID
                     GRTIVLGEVGLAGEVRAVSQMEIRIKEAARLGFTRCLIPKTNAAQLEKTAKIDICKIG
                     SLKEMLDYLF"
BASE COUNT         4881 a         5439 c         4638 g         5605 t
ORIGIN      
        1 gggataattc cacttgcgct tcgagcttca cttcgttcgc tctcagcgag tgtcattaaa
       61 aaaaaagcac ctttttaggg gtgcattttt tgagcagact gcttcacggg tttcttgtct
      121 gtgccgtata atgatgactc cgtgcgtctc attacggcag tcaggaagct tattttggaa
      181 atctcctgaa cttcctgata tatttttttt tcttccgtca tctgtcttgt tgggtctggt
      241 gggcccacct ggagtcgaac cagggaccta ccggttatga gccggtggct ctaccagttg
      301 agctataggc ccgctccttt tccgagtatc ttctaaagag tgagttgata tacacaaata
      361 ctcatattgt caataacatt ttattatttt gtgtaacgtt ttcgcgtttt attcttcgtg
      421 ccgctcatcg acaaagcatc tgcaacttcg ggcatctcag gcgtttccgg aagttccggc
      481 atcttttcaa gagtatctgt cacaatcata tcctcccggg tgaggatatt tgatcgatcg
      541 ctgattccga agcgcatgcg taaaactttt tcctcgcggg atgtcaatgt tgtcagaatt
      601 ttccgtgtct gctcggccag atcgatgtta atcgtcgctt ccgaaggaga catgactctt
      661 ttatcctcaa taaaatcacc aagatgactg tcttcctcct cgccgatggg cgtttccaga
      721 gaaatgggtt ccttggcgat ttttaaaacc tttctcacct tctccagcgg atactccatc
      781 ttattggcaa tttcctccgg agtgggttcg cgtcccagtt cctgcaccag ataacgggat
      841 gtgcgtatca gtttattgat cgtttcaatc atatggacgg gaattctgat tgtccgggcc
      901 tgatcggcaa tggcacgggt gatcgcctgc ctgatccacc aggtagcgta agtggaaaac
      961 ttgtagccgc gctgatactc gaatttatcc accgctttca tcagcccgat attgccttcc
     1021 tgaatcaggt caagaaactg caatccgcgg ttggtgtatt ttttggcaat gctcacgacc
     1081 agacgcagat tggcctccac cagcgtccgc tgggctctct ccgcttttct ttcgccgtct
     1141 tcaatctgcc tgatgattgt tttaagcgtc gttgccgata tgccgacttc tttttcgatt
     1201 tgttttatct tcttctgcgc ttccttgact gccgcgtcat tttttccggg tgcctttgcc
     1261 tgaaccgctt ccttccgcga ggtccctttt ttatcttttt tcgagcgggc ggatgttttt
     1321 gtttctttcc tgtacggtga aataatcttc tccgcagcat caatctcgtc tgcataattc
     1381 cgtaagcgtt gaataaaacg ctggatttgt ttcttgctaa aacggatctt gcgacagagc
     1441 ctgacaatga attctatgcg tcttttcaat tcggcatgca gtgtatcttt tttcttttgg
     1501 gtaagatttt ttgatttgag tttaacacgc gtcgctttga taatatcgta atgttcccgg
     1561 attttattga tttgcttcaa aatacggttc ctatgctcat cttcctcaat gaagccttct
     1621 tcatcttcga cgttgtcgac aacattcttg cttttgagtt cgccggatat cagtttctct
     1681 ccgatagtca gcacctcatc gatggaaatc gacaggcgga agatcgccgc catggtttcg
     1741 cgcgcgccct cttcgatcct tttggcaatt tccacttcac cttcacggct tagaagggat
     1801 accgacccca tttcgcgcag gtacatcttc acgggatcgc ccgttttttc agcactgggt
     1861 aaaatggcca gggcttcctt aagattcacg tcggtaattt ccttcatcga cttcttcagg
     1921 gtcagctttt cgccctgttc cgtgtcaatg atatcgatgt tcttttcacc gaagagtgta
     1981 ataatatcat ccagctgttc cgcggatgtc acatcgggca acagggcgtc attgacatca
     2041 tcgtaagtta aaaaaccttt ttcttctccc agtgaaatta acttattgac ttcgtcggac
     2101 tgaacttctt ttgccatctg gcatctcctt caaatgtttt ttttaacccc ggttccataa
     2161 acacgacgga acaaatccgt tgaacccttc cgcgtcgcga aagccatcct cccgtcgttc
     2221 attcaacctg ttgattgcaa ttccttttcc tttatgagca tttgctcttt ttcataaagc
     2281 agctgatgaa ccatctcttc gttgccgcta tcctgcgccc ggcttaattt tattttgatc
     2341 tgacgatgct gctccttaaa ccaccgtttt ttaatctgcc ggatgttatc ggtcaatatt
     2401 tttcccagca catcatcctc ggccggcaag gctttgatca taagctgaaa tattctttcc
     2461 cgcaatgcct gattttcttc ggtcgataaa atgacaccga catccacgaa gccgaggagt
     2521 ttgtaggcat cgatgatttt ttcccccagt tttcggatat cgggctgtag aaaataatcc
     2581 agcacattct cgttttccac ctgaacgatt ttctgaggat attccatcat cagttgaatc
     2641 aaatgcaact ccaccgtatc gtgatgaaca ttcacagccg ggttattccg gtttgtttcg
     2701 gtcctccccg aataaacacg gctgtaggct tctctgatca gcaattcttc attcaaaccc
     2761 gttttttccg ctatacgttt gataaacagg ttcttttctt tttgatcgcc gattttgctg
     2821 agaaacgtca tcgccgtctt gaccatatct cttttatctt caaaggtcct gccgcccccg
     2881 atgacatttt caatataata atcgctgagc ggctgggcaa gagcaatcag atcttccatc
     2941 ttatcctggc cgaatttccg gacataatca tcaggatcat atccgtcggg caagattaaa
     3001 gcccgggccc tcatttccgt acttaaaaat aattccagac tgcggtcaag cgcttttttc
     3061 cccgcttcat ccggatcaaa cagggcaacc acgtccacgc aatagcgtcg cagaagatcc
     3121 agatgatccc tggtcagcgc cgtacccaga gtggcgacaa cattacggat tccggcattc
     3181 cacaatgaaa tcaaatcgaa atatccctcg acaatcagcg caaaatcctt ctttcgtatg
     3241 tcgtctttcg ctttattcaa tccgtaaaga tttttgccct tgatgtaaac cggcgattcc
     3301 ggtgaattca gatatttggg ttcgcccttc cccaaaattc tcccgccgaa agccacgatc
     3361 tcaccgaaaa cgttttctat gggaaagatc agccgccccc gaaaccggtc ataaaaaccc
     3421 ccttccttac cgggtatgat caggccggcc tgttcggcca gtttcagcga aaggccgctt
     3481 ttttccataa aatcggtcag agagcgccat gtgtccggcg cgtatccgag acggaactgt
     3541 ttgacgacat cctctgcaat accccgctgc tgaagatatt ggcgggccgg agtaccggaa
     3601 gcagaaaaaa gattgcgcga aaattgctgc gccgctttcg aatttaagct gacgatattt
     3661 tccttgagca agtccttttg tctggcttcc tttccggagg aaaccggagg cagaacaacg
     3721 cccgttttca ccgccaggtc tctgatagct tccggaaaag tcttgtcggc aattttcatc
     3781 aggaacgtaa tgacattgcc cccttcgccg caaccgaaac agtagaagat ctgcttatcg
     3841 cggttcaccg taaaggaggg cgtcttctcc tgatgaaaag ggcaaaggcc gataaaattc
     3901 cggcctgcct ttttcaaggt gagatattcg gaaacaagat cgacgatatc caatctgttt
     3961 ttgatttctt caatcttgct ttgatcaacg tacacgattc atcctgtttg tccgcttttt
     4021 atcagcctga agaacggaga tgttattggg ataaaaaagc cttcactttt tcaccaaccg
     4081 ccttgccgtc agccacaccg gtcagtttgg gcatcaggat tttcatcacc ttgcccatat
     4141 ccttcacgga aacggcgccg gcatcctcta tcgttttttt gatcaacgca tcgacttctt
     4201 catcggacat ctgggcaggc atgaattcct gtaaaaactt cagttctgct tcctcttttt
     4261 ccaccaaatc agtccggcct cctttggcaa actgttcgat ggaatctttt cgctgcttga
     4321 tcatcgagga gagaacctgc agcatctcgc tttcatggag cggacgcatc agatcgattt
     4381 ccttgttgtg cagagccgtt ttcagcatcc gcgccgcgga aagcctgatc ttgtctttgg
     4441 ctttggcagc ggcaaccatt tcttcactta atttcgcatt gatatccatt ttttcacctt
     4501 ccattcgtta tcccaattcg tcctgcaatt ttctgccgcc gagcacatga acgtgcaaat
     4561 gaaaaaccac ctgtccgccc tcggcattgc agttaatcgc cgtgcggaaa ccgctttggt
     4621 cgattttttt aattcgcgcg actttctgcg cggcagccgt cagtcgatgg atcacatccg
     4681 agttatcaac atcgacatcc atcaacgttg cgatatgctt tttgggaatg attacaacat
     4741 gcaccggggc catcggatga atatcatcaa acgccagtac atgctcgtct tcaaaaaccc
     4801 tggcacaggg gatttctccc cggacaattt tacagaaaac acaatccgac atgaacgcca
     4861 cctcctcttg ttacgtgcgg gtcacttttt ttgttttgct tattgcctct tccaggtcga
     4921 cggcgccggt atacagcgcg cgaccgataa tcactccgaa aatgctgccg ccttcgacga
     4981 ccatcaggtt gtccacatcg gcaatcgatg ccacgcctcc tgaggcaatc accggaatgc
     5041 tgacggaatc ggccaattct cttgtcgctt tcatgttgat tccggttccc atgccgtcgc
     5101 gctcgatatc cgtgtaaacg atggccttga cgccataggg ttcatactgc cgggcgaggt
     5161 caaccgcatt tttctccgtg ttttccgtcc agccccggac ggcaactttt ccattgaggg
     5221 cgtcaatgcc caaaataatt ctgccgggaa aaaccgtaca ggcttcgcga acaatctgtt
     5281 gattcttcag ggcggttgtg ccgagaatga cactggagac accctgatcc aggtaaaatc
     5341 ggatcgtctc catatcccgg atgccgccgc cgatctgaac gggaacattg atgttctcca
     5401 cgatattcag aatgatatcc gcgttgcgcg gtcttccggc aaccgaaccg tccagatcaa
     5461 cgatgtggat caattccgca ccctttcggg cccagagcag cgccatatcc aggggattgt
     5521 ccgcatacgt tgtcacacga tcaaatttgc cctgcgccag cctcacacat ttcccatctt
     5581 tgatatcaat cgccggaata ataatcaatt gcttcactcc ttcaaaataa gaagagccct
     5641 gaacggttca gtcggaaatc acggcatgtc acaaaaacag caaggcttaa ttttttcgtc
     5701 acattgtcta aaacccgcca cggaggggtt tcacgatccg ccgcatccgc gtatgatgga
     5761 ttaaggctga aaatccggaa acgtctggaa aacgttctcc atgcccagtt ccgtcagttc
     5821 gcgggccgtc atccactttg cgccgccctt gaaaaacgaa gagatgtcca gaacattttc
     5881 catcagagat cgttgcgttt tatcaaaata tccccgccag acggatttgc cgtcggcagt
     5941 cctaatcaaa tgaatttcaa aaacaacaga cgccggatgt tccgatgaat accggtagcc
     6001 caccctttcc gcaaaacggt aaacataacc gacaaccagg aaatcagcct tcaattccct
     6061 gccgatcttc agcaggccgg ttgcttgaaa atctttcatg ctgtcggtag aatatttttg
     6121 agtcgctttc tgtaattttt ccgcgggtac caacacgata tcgtctcttg ccttcagttg
     6181 ttctacaaaa atttcttcaa cgattttttc cgccccctcc tgtatgatgc cgccggccga
     6241 agccttcccg caaatcggac agaggacggt atttccggtg ccgccttccg gccttaacgg
     6301 ctgaaaggcg atgaccgcta tcagattttc gctttcatcg ctctgtgctt ttttatccag
     6361 cgcttgcgac atgacaggaa aagccagcaa agcgacaaaa acaaatctca caataaaaca
     6421 aaaatatttg cggagaatgg caaccatgat ctcacctcga tgtcttgact cccgatcaac
     6481 gggacaggtc ccgccgataa gggatcggtg tatgaatgac gctgctttcc acagaaaagc
     6541 agaatccaac cggctaaagc tttcctttgg tggaaagaac gcccttgatg ttttcgttta
     6601 gggacacggc atttctgaga gcacggccga aagccttgaa tatcgattca atcatgtggt
     6661 gaatgttttc gccatacaga aaattgatat gcagcgtgat gccgctttga tccgtaaacg
     6721 ccttgaaaaa ttctttgacc agcgccggat caaatttgcc gatttttctc cggccgcttt
     6781 tcacgtgata gatcagataa ggccttccgc agatatccat atccacctgg cacagacatt
     6841 catccatcgg cacacgggcc gatccgtagc gattgatgtt ttttttgtct ccgagcgctt
     6901 tagccaccgc ttggcccaga cagatgccca gatcttccac cagatgatga tcgtcaatat
     6961 gcgtgtcccc cttgcttctg acgatcagtt tcaaaaaccc gtgacgcgca aacagctcca
     7021 gcatgtgatt aaaaaacgga atggtggtct caatgctgct gccggcggtt ttatccagat
     7081 cgatttcaag acgaatatcc gtctctgttg tcttacggac aatcttcgct tttctcgcca
     7141 taatcgttgt ctccgctaaa taaccttgtt taaaggatat tcaataatac cctgagcgcc
     7201 tgcatccaga agtttcggaa tcaaatcccg catgactttc gcgtcaacaa tcgattcaat
     7261 ggcaaaccat ttgtctgaag acagaccgga aatcgtcgga gatttcagtg aggggatcag
     7321 gccgataacg cctttcagat tttctttgga cacgttaagt ttcagaccca ccttccccat
     7381 ggcctgcaaa gaaccggtca gcatggtgct gatctgctca atctttttgc gtttccatgc
     7441 atttttccac gcagcgcgat tggcgattaa ctgagtgttg gttttcatca gttcatgaat
     7501 gatgcgcaat ttgttggcct tgattgtcga gcccgtttcc gtcacttcca caacggcatc
     7561 caccagcccc tcgacaacct tggcttcggt ggctccccag gaaaactcaa catgaatatt
     7621 gatgtttctt tccgcaaaat atttcttcgt aaagttgacc aattccgtgg atattctctt
     7681 tccctccata tcctcgatct ttttgtaggg agaatcgtca cggacaacca gaacccagcg
     7741 ggcctgctgc gtggacacct tggaataaat aagatcctgc accacaacga catccgaatc
     7801 attttccatg atccagtcga tacccgtcag gcccagatcc agcgttccgt cttcaacgta
     7861 acgtgacatt tcctgcgccc ggacaagctt gcaagaaatc tccggatcat cgatatcggg
     7921 aaaatagctt cgggcgtcgc tggtgatgcg ccatcccgcc tttttaaata aatcgatggt
     7981 attggcttcc agactgccct tgggaattcc cagttttaaa tttttcattt atacacatcc
     8041 tctggattaa aaatcttttc tccgacggca acaaaatcat ttccttccaa cttgcggtaa
     8101 aaacaggacc ggtgaccggt atggcaggcg gcatctccta tctgttggac atgtaacaga
     8161 atggtatctt catcgcagtc gataaaaacc gctttgacga tctgaacatt gcccgaggac
     8221 tcgcctttca accacaattt tttccgcgac cggctccaat aggtcgcctt tcctgttttt
     8281 aaagtcgcgt cccacgattc gcgattcatg taagccatca tcagaacgtc tttcgtttcg
     8341 gcatcttgga caatcgcggg aatcagaccg tctcctttgg caaaatcagg ctctatcatg
     8401 ttcacctcat atctttatga ttttatacgt ttaaatgatg aacagcttta aatcaagcaa
     8461 tattttcaca tttcacagca tctgtttgca tcttgccgga atggtctttt tatgatagaa
     8521 aaaaaatgtc ttcaaatcat catcttgacc ggagggaaca ctatgtcgtt gctcatcggg
     8581 ggaatcattt ccgctatctt cggattggtc agtttaattt tcttctggcc ggattttatg
     8641 acgattttta aaggatccct tccgattttc ctgctgctcg gaggagcatt ggccgtttac
     8701 gtgggcatcg atgaaatcca ggaaaaaaac aggcaggaaa gacacctgca ggaagaaaaa
     8761 ctggaacacg cgcaaaagga aatcgaaacc atccgggctc aggcggaaag ctacagggaa
     8821 gaattgcaga aactcaaaga ctcaaaacaa ccgtaaattt ccgccacata aaatgctgtg
     8881 acctgcaatc cacagtgcaa ggccgtaccc ttctcaacgt ttaaaacgtg atgatcagac
     8941 agcctctcgt tttcgtcccg cgaccatacc ccagatcatt aacacagcac cgatggtgat
     9001 ggctgaatca gccacattaa atgccggcca gtgggctgtt ttgatgtaaa aatcaagaaa
     9061 atccacaacc gctccaaagc gaatccggtc gatcaggttt ccgacggcgc ccgcgaagat
     9121 catggtcagg gaaacgacga agaacatgtt ttccgatttg cttttcgcga tgtaataaat
     9181 gatcagagcc atgaccagca gggtcatgcc gatgaaaaac agagagcgaa acatctcgga
     9241 catgccggcc agaaagccga aggcggcgcc gggattcatt acgtaaacaa gattaaaaaa
     9301 accgtcaatc accgaacggg tttcatgcag aaagaatttt tttacgatag ccgctttcgt
     9361 gatctgatcc agcacgacga tgacagccgc tcccagaaca aaaatgataa tattcttttt
     9421 caaagagaac tccttttttt acagattcgg cagacagcgg gcacagatgg tcggatgctg
     9481 cgcgtcggca ccgacggact cgtcataaat ccagcagcgt tcgcacttgg cgccgcgggc
     9541 tttctcaaca ccgaccatga gcccttcaat ttctgcactc cggaagggtg cggcaatatc
     9601 ctgctcatcg gccagctgca gctgcgacac aatcagcagc gcttttaagt cctcgagatg
     9661 cgcggcaaac aacgcccgca gtttttccgg cgctgcaatc gtgatgcgcg catccagcga
     9721 atggccgaca attttctctt ttcgcgcctg ttcaacggcc ttggcaattt cactcttcgc
     9781 gtcgatcatc gctttccagc gctcgcccag gtccgcattg aaaaaggtgc cgctgacttc
     9841 cgggaactgc gccagatgaa cgctatcttc cttgttggtc cagtccggca tcgccgccca
     9901 gacttcctcg gacgtaaagg tcagaatcgg cgcaagcaat ctggtcatcg cgttaagaat
     9961 gataaacatc gccgtttgac cggagcgtcg caccgcggaa gcggccttgt tggtgtagag
    10021 ccggtccttc aaaacatcga gataaagcgc gctcagatca acggtgcaat aattgtaaag
    10081 cgtgtagaaa accacgtgga actgataccg ttcataagcc tccgtcacgc gtttgatgac
    10141 ttcctgcaaa cgatgcaggg cccaccggtc cagctcggac atctgctcgt acgccaccgc
    10201 atccttatcc atgttgaaat cgtagaggtt gcccagaatg aaacggctgg tgttgcggat
    10261 ccggcgatag gcttccatca ggcgtttgag gatttcttcg gaaatgcgga tgtcgtcggt
    10321 gtagtcttcc gccgccaccc acagccgcag gatttccgcg ccgtatttat tgatcgcctc
    10381 ttccgcgccg atgacgttgc cgacggattt ggacattttt ttgccttccc cgtccacgac
    10441 aaagccgtgt gtcagaacat tcttatacgg cgcgcgtccg cgcgtaccca cgctttccag
    10501 cagggacgaa tgaaaccagc cgcgatgctg atcgttgcct tccagataca tgtcgcaggg
    10561 cgaactcaga tccggacgtt tttccagaac ggccgcgtgg ctgactcccg aatcgaacca
    10621 gacatccaga atattcactt ccttggtaaa ctccgtccct ttgcagtgcg gacaggtcgt
    10681 atgcttcggc atcaggtctt ttggatcttt ttcaaaccag acatccgctc cgtttttttc
    10741 caccagcgcc accagatgat ccatcatttc cctggtcatc acttcatttt tgcatttggc
    10801 gcaatagaac acggtaatcg gcacgcccca caatctctgg cgcgagatac accagtccgg
    10861 gcggttttcc accatgccgt agatgcggtc tttcccccac gagggaatcc atgtgacttc
    10921 gttaatgcat tccagcgctt tcttgcgcag atcgtttttc tccatcgaaa tgaaccactg
    10981 ttccgtggaa cgaaaaatga tgggtttctt gcagcgccag cagtgcggat aggaatgctg
    11041 catcggcaca tgccccagaa gcgcgccgac ttcatcgagt ttcttgatga cgctgtcgtt
    11101 ggcgtcgaaa acaaactgtc cggcaaaatg ttcgacatct tccgtgaatt ttcccgcgtc
    11161 gtccaccggc gcgtaattat ccaggccgta ctcgaggccg atttcatagt cttcctgacc
    11221 gtggccggga gcaatatgca cggcgccggt tccggcatcc agcgtgacaa acggcgccag
    11281 gatcaacaga ctgtttctct taatcagcgg atgaacgcat ttctgacctt ccagaaccgc
    11341 gcccttaaac tcatcgataa tttcatagga cttgccctga tatccaaagg cgtccagaca
    11401 ataatccaac atgtctttag cgacaatcat tacgtcgtca ttgattttca ccgccacgta
    11461 aataaaatca tccttgacgg caatcgccag attggccggg atcgtccagg gcgtcgtggt
    11521 ccagatgacc atcgccactt tctgggcggc cagtttgggc agcacctggg caatgtcgga
    11581 gacaaaatta aatttcacgt aaatggacgg cgtgtgatga tcggcatatt ccacttcggc
    11641 ttccgccagc gcggttctgc aggtggcgca ccagtaaacc ggtttttttc ctttataaac
    11701 actgccgttg agataaagct tcccaaattc ctcaaccgtg accgcttcgt aaggataggc
    11761 catcgtcaga taaggattct cccactcgcc gaaaacaccg aaacgcttga actgttcacg
    11821 ctgaatgtcg acatattttt ccgcataggt cctgcaggcg cggcgctttt ccacctgcga
    11881 catcgccccc tttttggcgc ccagttcctt atccacctga tgctcaatcg gcaggccgtg
    11941 acaatcccag cccggcacat agacaccgtc aaatccggtc atgttttttg atttaatgac
    12001 aatgtccttt atgattttat tgagagcggt gcccagatga atattgccgt ttgcataagg
    12061 aggaccgtca tgcaaaatgt aaggcgtttt ccccttggcc atttgtctga tctttttata
    12121 gatgttcatg tcttcccatt ttttcagcat ttccggctcg cgctgcgcca gattcgcttt
    12181 catcggaaaa tccgtctggg gaagattcag ggtctttttg taatccattg tttgctcctg
    12241 tatttaagtg atcacgtcac gaataatctt tttacaatct aaaaccagga caacctctgc
    12301 aggcgccctg gttaagttga agtgaactac catattattc cgacacttgc aagcaaactt
    12361 catgagatcc aggcgtcaca gacagattga ataacccgat aagccgaaca atctcaatgt
    12421 ctccatatgg tcaggcgaac ccacctgcca tggaaacggc tacagacaag tctcttaaaa
    12481 tttatatttt ccggccatga tgtcctgata gatggcgtca ttcaggggaa cgtatgtttc
    12541 ggttccgggc aggagaacat acaggccgtc tttgatcgcc gacagattat atccgtcatt
    12601 tttcagatcg ttcttcttga tcttatgcgt tgccgtccac tggaaatcat tgacaaaacg
    12661 gataaaaatc ggcaccgcgt atttgggcag aacggaccgg aggtgctctg ccagcgcggc
    12721 aaaatcaaca tctttccctt cctgttttat cacggcggcc ataccggctc tgccgtcgcc
    12781 tttcggcata accacgccat aaaccgccga agcggacacg ccggaaaaag aatccagcgc
    12841 tttttccact tccatggttg cgacgttttc gcccttccag cggaaggtat cgccgaggcg
    12901 atcgacaaac tgcgcgtgac gaaatcccat attgcgaagc atgtcgccgg tattgaacca
    12961 gagatcgcct ttcctgaagg cattgcggat aattttctct tcggttttct ttttgtcggt
    13021 ataaccgggg aacggggttt tttcagatat ttccataatc aggaggccgg ggtcgccttt
    13081 ggcgactttg ataaggaagc ccccggcatc gcgcgccgga caatcgtttt cgatatcata
    13141 ttggacaatg gcaaagggcg tgagactggt tcccacggta taatcaaaat tcataagatt
    13201 ggtaaacacg ccgacccctt cggccgctcc gtaaaattcg tatattttgc tgatgccgaa
    13261 acgcctcttg aaagccatcc agatatccgg tctcaggccg ttgccgataa tgtatttcag
    13321 ggtggtcttg ttttcgccgg ctttttcggg ctgtgccatc aggtagcggc acacttctcc
    13381 cacgtagaca aaatgcgtgg cctggaattt cttcacgtcg tcgagaaagc cgctcgcgct
    13441 gaatttccgc ctcatggcaa ctgccgctcc gctgtacagg gccggcggcc atcccacggt
    13501 gatggcattg gtgtggaaaa aaggcagggg aatgtagatg gtgtgtttcg gcttgacatg
    13561 cgttacgacc cttccgaacc agaatgtcgc ggacatagcc cgcttatggg tgatgaccgc
    13621 cgctttgggc aacccgccgg ttgttcccga ggtatagacg taggccatcg tatctttcag
    13681 gctgatcccg cccgtttcgg aaagattggt tttgggcagg acggcaagct gatccgtaat
    13741 gtcttcgggc gcctttaatt gattcattgc cggatccctg agcaaacaaa gctccgatcc
    13801 ctgaaggtcg attcggtcct tcacgtcctt gaagaaactg tagcactcct ctccgaccac
    13861 catcaccttc cccttgttga gattgaaact gtgtaccaga gaatcccctc tctgatttgt
    13921 attgatgaga gagcatacag cccccacttt tgcgcatccg caataaagaa aaagcagttc
    13981 cggcctgttt tcaagacata ctgcaaccgt gtcgcctttt ttaacgtttt tcgacagcag
    14041 aaaatgggca aactggttgg ccttctcatt aagttcggca taggtcaatg tgccgtcggg
    14101 tgatttcacc gccgggttgt tcggatactt gagtgcggat ttttgcagaa gcgatcccca
    14161 ggaggcattg cgattgctgc taatcgattt gatccccttg atcccgtgat agacgacaaa
    14221 tggaatccgt ccaacgaacg ccatgaactt gacaaaaaat tcaaaaccgc tgatggtttg
    14281 aggatactgt acgacatcgt tttttttcat tttgattcct cctggttggc cgctttgaat
    14341 aatgattgaa tgtgcagata tcttgaaaca agatagttgt ctgtgtcaag caaattcggg
    14401 gtgccggttg agtccgtccc cttggggaaa aaacaatacc agcgacaatc aaagcgccca
    14461 taatccgatt ccttccgccc aatcccgcaa aaacatcttc cggagaaaaa caatccggga
    14521 ctttttcacg ataaagactt gacatcccgg ttcttgccat taaaataaca aaaagaattg
    14581 tcaagacgta ccgtcaatga ctgcccgggg cagtttttaa ctcaggaggt taaaaatgaa
    14641 aagaattttg ttgtttctcg cgaccaatat cgctgtgctt gtcgttttga gcattgtttt
    14701 gcgtctgctc ggtgtcgacc gtattctaga cgaaagcggc atgaatctta atatggttaa
    14761 cctgctcgtc ttcgcggcgg tattcggttt cggaggcgct tttatatcgc tggccatgtc
    14821 caagtggatg gccaaacgca tgatgggcgc gcaagtcatc accaatcccc aaagtgcgac
    14881 tgaaacctgg ctggtggaca ccgtgaaaaa acatgctcag atggcgaata tcggcatgcc
    14941 ggaagtcgcc attttcaact ctccgtcccc gaacgctttc gccaccggaa tgaacaagaa
    15001 taaggcgctg gtggccgtca gcgcgggact gcttcacgcg atgaacaagg aagaagtcga
    15061 agcggtcatc ggccatgaaa taagccatgt ggccaacggc gacatgatca ccctgtccct
    15121 gattcagggg gtggtgaaca ccttcgtcat tttcctgtcc cgcattgtgg gtcattttgt
    15181 ggaccgggtc attctgaaaa atgaagaaga aggccacggc atcggatttt tcgtcacgtc
    15241 cctcgtcgcg caaatcgtct tcggcatcct ggccagtgtg atcgtgatgt ggttcagcag
    15301 aaaacgggaa tttatagcgg atgcgggcgg cgccaaactg gccggaacgc aaaacatgat
    15361 caacgcgctc gaagcgctca aaagaggaac cgctgaaccg ctgcccgatc agatggcggc
    15421 tttcggcatc aacggcaagc cttccggcca cgggctcaaa cttcttttca tgagccatcc
    15481 gccgcttgag gatcgcatag aagccttaaa aaaactgaca taccgataaa ttattaaaat
    15541 atcctcaaaa aaggccggtc cgtttcgtgt aaaaaaacat gtaaaaacgc accggctttt
    15601 ttgtttgaat ccggccgggc ggacgaaccc gatctgtaaa aattttttga cctatgactt
    15661 cttcaaacaa caatgattgt tctttgaaaa ttactgtttt aattataaat aaattataaa
    15721 atattcttga caactccttg caaataacgt aaaaaaccga caaggcttgt tttagacatt
    15781 cagagcctta aatcttacag aaaaaaatta aaaaatatta ggtgtcaact cttaaaaatt
    15841 ttttaaggag aagaatatga agaagattgt ggtttgcgtt gtcgtcatga tgttccttgt
    15901 aacgttgcct tttgctgctc tggccaagaa agtgtctctg actgaagaag aactttcatc
    15961 cacgacagct caggaaggtg tgactattca gttcgggaaa aacacttatg gaggagatta
    16021 tttctttgac accaatttct acgttgcggg caaatttctt cccaccattc aatcatgggg
    16081 agacggtgac ggttgtaccg attgcggcgg ctacacagaa aaaggctgga tcggcgtcag
    16141 aaatgtaagc atggatcctt tcgtaagctt aggacttcct aatcctgctt ttcctcccac
    16201 aggttctttt atttctcttt acaggacaat gaagattgac gtcggcacca gcggcggcgc
    16261 aacaaaatta ttcatcggca tgccaagtat tatggtccat ccgtcaggca tttctcagac
    16321 attaagttcc ggcacaacag aaacgcttgg acaggatttc ggcacagcct acatgagtga
    16381 attcgccctc atcgcaaatt ctgcaggtac cggctatctg cgaatcggaa accactcctc
    16441 cacaggacag cagggggtgg agattcaatt cagcagcact tctcctgttg ccgccggtcc
    16501 tctgtatggg gttgttttct caattccggg caaacccatt gttcagtcct ggagcgatat
    16561 cgacggtgag caaacaggtt cgggctacac gaacgcgggt tacttcggtg caaaaaactt
    16621 tacgatgtcg gatggaacga catacgcatg gcctggaagc ccctcaactt tcaacatgat
    16681 cgttaccggt acgttgtcta ttgatgtcgg caccaacggc agcggtcaaa cggctgttgt
    16741 gctcggagtg cccacggtat tctttgcccc gaatgcagcc ataacgtccc cgtttggatt
    16801 tgccacagac agagagttga atacaaactg gcagtcttta ggcacattat atactgccgg
    16861 aatagacatt catcagatga ggggcagcct ggcgatatcc gctcattaat tattcttctt
    16921 aaagttagct taaataacca tgtgtttttc aacacatggt tattttttta tctgattcag
    16981 atagccaaca atcctgaaga cctgaaaaac gcaaattcca atatattcat tctccgagac
    17041 tgtgtcgtca ttactttttt gtcattccgt gcaagcgaag cgcgactttg agcttgcccc
    17101 gtgcttgaca cgggggaatc cggtaatttc gccaagttcc ggatgccggt tttcaccggc
    17161 atgacggtat ggatagcact ttctgctatt acgaaacagt ctccgccgcc ggggtgacga
    17221 ctgaactgac cacgtgccca atatcgttac tattgttgga ttattataaa gataaatcaa
    17281 tcggatgtct taaggatcaa aacttcccgt ccccgacaag gaggcttttc attttcaaca
    17341 cagaaagtcc cctttccgga acatcatcaa tccggttgcc aaccggattg atccgaataa
    17401 cattcgttca cagaacacaa atcagggtat ttttctttaa aatttgagtc tcccaataaa
    17461 aaaagccccc cgtccgattg gacagggggc tttaacttta tatattagag tcgcgtcgca
    17521 ttcgcccgga taccgaggcg gcgaggagga ggctccgcag gcgtattact aatacgttga
    17581 ggaagccgac gacgctgcca acaaaggtag ccgtgtgaat gccgtcgtga ctacattccc
    17641 attccgggat atccgccacc gggaggcata ccgggcattc caccgccggc gcccttctct
    17701 tcaggcttgt cggcgatcat gcactgcgtg gtcaaaagca gtccggaaac gctcgccgcg
    17761 ttctgcaggg caaaacgggt aaccttggtg ggatcaatga caccggccga aatcatatct
    17821 tcatattcat cggtgcgggc attgaaaccg aaggcgccct ttttctcttt gactttttcg
    17881 accacaatgc tgccttcgag gcccgcattg caggcgatca tcttgatggg ttcttcaatg
    17941 gcttttttca cgatattgac gccgatctgt tcgtcgccgg taagatccat cttggacagt
    18001 gcgggcaggg tgcgcagata agccacgccg ccaccggcaa cgattccttc ttccacggct
    18061 gcacgggtcg catgcagagc atcttccacg cgggctttct tttccttcat ttcggactcg
    18121 gttgcggcgc cgactttgat aacggccaca ccgccgacca gtttggccag tctttcctga
    18181 agtttttctc tgtcgtaatc ggacgtggtt tcttcgatct gagcgcggat ctgtttcacg
    18241 cggccttcga gttcggcgcg tttgccggcg ccgtcaataa tcgtggtgtt gtccttgtca
    18301 atggtgatct tcttggcgcg tcccaaatct tccagtttca cattttccag tttgtagccc
    18361 atgtcttcgg agatcatctt gccgccggtc aggatagcga tgtcttccag catggccttg
    18421 cggcgatcgc cgaaaccggg agccttgacg gccgctacat gcagggtgcc gcgaatcttg
    18481 ttgaccacca gcgtggcgag cgcttcgccc tcaacgtcct cggcaatgat gagcagcgga
    18541 cggcccatct tggcaatctg ttccaaaatc ggcagcaggt ctttcatgcc gctgattttc
    18601 ttttcgtaaa tgagaattaa aacatcttcc agagcgacca gcattttatc ggcgttggtc
    18661 acaaagtaag gagacaggta accgcgatcg aactgcatgc cttcaacgat ctccagttcg
    18721 gtttctaggc ctttggcttc ttcaacggta atgacgcctt cctttccaac tttatccatg
    18781 gcgccggcga tgatgttgcc gatggtttca tcgttgttgg cggaaatggt gccgacctgg
    18841 gcgatttctt tctgatcttt ggtgggtttg ctcatcttgc tgagctcttt tacgacggtc
    18901 tcaacagcct tatcgatacc gcgtttgatg tccatgggat tggaacctgc ggcaacagcc
    18961 ttgacgcctt cacggtaaat ggcctgagcc agaatggtag cggtggtagt tccgtcgccg
    19021 gcgacgtcgc tggttttgct ggcgacttcc ttgaccatct gtgcgcccat gttttcaaat
    19081 ttatcttcga gttccacttc tttagctacc gtgacgccgt ccttggtcac cgtcggggaa
    19141 ccgtaacttt tatcgataat aacatttcgg cctttcgggc ccagtgtaac tttaacagca
    19201 tcggccagtg tattgacgcc cttcaaaacc gattttcttg cttcttcatc gtaaagaagt
    19261 atttttgctc ccattgtata tattcctcct tagtagtttc ttatttttcg ataatgccga
    19321 gaatgtcgtc ttctctcatg atcaggtatt cttcgccgtc gatcttgact tccgttccgc
    19381 cgtatttgct gaacaggact ttatcgccca cgctgacttc gggcttgatg agtttaccgt
    19441 catcggctac tttgcccttg cccacggcaa tgactttacc ttcaatcggt ttttccttag
    19501 ctgtatcggg aatgatgatt ccccctttgg tcttctgctc ttcttccaaa cgttttacaa
    19561 tgattctatc ttgtaaaggt ctgattttca taaactttac tctcctttcc tgatttttaa
    19621 aatttaagaa tgctgtttta acagacgtat tttatgcacc atttttgaga tgtcaagggc
    19681 acggagggga tttatattaa ttaaaataaa tagtccagca tttctttcag ggaaccgatt
    19741 ttacaaatat cgattttggc tgttttttcc agttgagccg cgttggtttt cggaatcaga
    19801 caccgcgtga agcccagacg cgccgcttcc tttatcctta tctccatctg agagacggca
    19861 cggacttcgc cggccagccc cacttctccc aaaacgatcg tccggccatc aatgggcttg
    19921 tccagaaaac tggatgccat ggcggcaacg atgccaagat caaccgccgg ctcttcgact
    19981 tttactccgc cggcaacatt aatgaatata tccgacccgc ccagatgcag gccgcaaatt
    20041 ttgtccagga ccgccaccaa aagcgatacc cggttataat ccacaccgat ggctgtccga
    20101 cgcggcgtcc cgaaacttgt cgagctcacc agcgcctgaa tctccaccag aatcggcctg
    20161 gttccttcca gagagggcac gaccaccgaa ccggcaacat tttgcggccg ctccgccaga
    20221 aaaaaagtag agggattggg cacttcgatt aaccccttct cccgcatttc aaaaacgccg
    20281 atttcgttgg tcgggccgaa acggtttttc attccccgga caatgcggta agaatgagac
    20341 gaatcgccct caaaataaag caccgtatcc accatgtgtt ccagcacttt gggaccggca
    20401 atcgagccgt ctttggttac atgaccgacc agaaaaacag ggatgccgtg tttttttgaa
    20461 aaaagaatca accgcgaaga agtttcgcgc acctgcccga cgctgccggg agcggacatc
    20521 agttccgatg cataaaccgt ctggatggaa tcaataacta caa
//
LOCUS       DAOSME010000042        20482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3219_length_20482_cov_18.628384, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000042 DAOSME010000000
VERSION     DAOSME010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 20482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20482
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_20482_cov_18.628384"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLG98_07450"
     CDS             <1..295
                     /locus_tag="PLG98_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33126.1"
                     /translation="LKMAARDIGFMFSRRVFHKDLQLNNILLNPADPAQLHWIDNDLK
                     KIGRRRWAKQPKAVLEQITRNVQFCSIDEKNFFIKEIVSSWAIQKEEDDGQPS"
     gene            complement(310..999)
                     /locus_tag="PLG98_07455"
     CDS             complement(310..999)
                     /locus_tag="PLG98_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HOE33127.1"
                     /translation="MTAFCRRFVLAILMVAASTGPAWSAADDGWKKVGESDGIAGFTR
                     PTAKSSVDEIRAVGVVNAPVAVVEAVIRDISIMPQYMFLCKEAALINTPEMKSAGDAV
                     YFHSVTDMPFPVSDRDAVVKSVWSVDRKTGTVYCRAKGLKTTFRQNKDIVRMPLLIIE
                     CTLAPKGEDKTEVIYQVLGDPGGELPSWLVNMLTSDYGIKTIAGLRKMVSREKFRNAQ
                     KVITVTPHDQK"
     gene            complement(1039..>1438)
                     /locus_tag="PLG98_07460"
     CDS             complement(1039..>1438)
                     /locus_tag="PLG98_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33128.1"
                     /translation="AIDMTGKAGISTQGFFIFGLPGETAASMEDNIRFALRSGLSRAQ
                     FVILDVLPGSELWDSLRGKFTPNWSKESFRQPEYLPEGMTKEQLMQAQSRAFRKFYLR
                     PRILFRLLSLMDYRQIFYLLRRLKAYRILG"
     assembly_gap    1439..1538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1539..2555)
                     /locus_tag="PLG98_07465"
     CDS             complement(<1539..2555)
                     /locus_tag="PLG98_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33129.1"
                     /translation="MKIALVRPNYKSHIITPPIGLGYLSSYLKKSGIETKIIDGLKEN
                     LSPSRLMRRIQKFNPDAVGITCLTAFYQEVVVLARELAKNKITTVIGGQHPTFLPYRT
                     LLESQADFVVCGEGEIPLLELAESKFCNKSIRGVYSLENLKSEQQEIIKASVVDNLDD
                     LPFPDWEQIDPREYPRAPHGAVVRGFPVGVMVTSRGCPFECTFCAAPRFCDRTIRFRT
                     PENVVAEIEYLMKNFNISEVHFEDDNFTLNADNVKKLCRLMTERNIKIHWACPNGVRA
                     DNISEDLIALMKQSGCYYIAYGIESVHSSILKNIKKRESVETIRHAIDMTGKAGISTQ
                     GFFIF"
     gene            complement(2552..3265)
                     /locus_tag="PLG98_07470"
     CDS             complement(2552..3265)
                     /locus_tag="PLG98_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE33130.1"
                     /translation="MNKLYVVMPAYNEAGIIEKVVQEWHAVVDSVGHGSKLVVFNDGS
                     TDETSAILESIQTSHPHLTVINKTNTGHGPTCLAAYKFAVAEGADWVFQTDADGQTQS
                     GEFWRFWEKRDTCDFIIGWRARRADGPARVFISRILKGVLALLFNVSLKDANAPFRLM
                     KAERLRLYLPLIPDNFFIPNVLLSAMLTREGEKILWQEISFNPRNAGQSSIALFRFGG
                     LGIKLIIELYKLRHLKLVT"
     gene            complement(3298..4947)
                     /locus_tag="PLG98_07475"
     CDS             complement(3298..4947)
                     /locus_tag="PLG98_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33131.1"
                     /translation="MTALVFSGSLRLDWTNWDDNLLVYENSLVREARFIDIFTKPADY
                     NTYNPLVISSFALEWKLVGDRPFLYHFDNLILHLLCTGLVLLLFRRMGLSVWWSGVGA
                     LLFGIHPMRVESVAWITERKDVLYGFFYLSALLAYIRYIASGKAGYFLLTFCLFFLSL
                     LSKGQAVALPFSLVLLDWYFGRKMNGKAVMEKLIFFAMALLVGLLGTTFFFQNVYKTI
                     DSKAIVNAFNFFEQTVLAGYAYTIYILKFFIPYVTSTLYPVPPALHAGHWIGAITAVL
                     IFLWALAVRFQYRFVTFGLLFFTFNTLFLLMPFLANDTAYLNDRYIYMACVGLAFAAA
                     FSMQKMEERYPIRRLPVAATAVVVLLIFSVLTIKYIPVWKNSETLWTDVIEKYPRKIS
                     MAYFNRGNDRCVQNRYNDAIQDYTAAIDLNPQSLLAYQNRGLAYLISDDFNKALQDYN
                     RYLALRGPYDAGGSERDVHLSNVLGNRGVIFSRMNQFEKAIADFNTAVKLNPYNPNNY
                     LNRAFVFYRLGRMEQAAQDVRMAELTGRTVDPSFKKMLHMP"
     gene            complement(5097..>6184)
                     /locus_tag="PLG98_07480"
     CDS             complement(5097..>6184)
                     /locus_tag="PLG98_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33132.1"
                     /translation="RYSSVIAVVPPFAVLLCAEYFVTPEKKKFVMEKIGLISAGFFIP
                     LLAFFAYLFLHSALDDFSLQNTTIVRFIGEGDNVNTYLSFLIFLLQMENSLASDLRGK
                     IFTPVLLVCLFFAIKEIRQYVFSRLREPAYKDGALFAACLIAVFGYLNSIHVYETFRL
                     VNGASLGIGVCVLAAGYCFDKVGKPLKYLIPAAGLIVAAVLLSTLFFKQTTSAYYPWQ
                     RDVLFGRGIQNSSVPILKGKILTKEYNSFYEEVFDVIKPYKKCCYIVNYTRDVVAFVM
                     NDLPRVQISPVPIPGVDDISRQVQSIEEKKAVILSFKKLEIPGYVTVFAKPWPAEIPW
                     MGGGYFFIFAPERLTTDHEIYPDKEEK"
     assembly_gap    6185..6284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6285..6913)
                     /locus_tag="PLG98_07485"
     CDS             complement(<6285..6913)
                     /locus_tag="PLG98_07485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE33133.1"
                     /translation="MMSVSVLSVHENKSGHPQGVNKGFFSFDAFNGFDFLVLGICLIS
                     FVCSIIEARVNVDNLHWGWAYIAALDLKRGAIPHSEVIIFYGYIYTWIQSISLMLFGE
                     RLVSLGIVTGLFYSMSLFLSYRVFLRLMKRGLACLSVLLIFLIHPYAIYPAPNYFMYM
                     FELLALVYFLKYSESKYYGLLAGFFLSMSVLARYSSVIAVVPPFAVLLCA"
     gene            7143..7871
                     /locus_tag="PLG98_07490"
     CDS             7143..7871
                     /locus_tag="PLG98_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOE33134.1"
                     /translation="MNINNSNLHNEKIKYSLLLLSQRYNYNRWIYNNIQRFIGNNVLE
                     VGAGIGNLTDFILSKNKLTLIDIHQPYVDYLKAKYSFCDSAAFTVLQTDIQNIQSSPV
                     SGMTFDTVICLNILEHLENDRTAVENMSHLLQPKGKLIILVPALKTLYGSMDISFDHR
                     RRYNKKDLRSLIQDQNLDVLKLYYMNFPGMLGWFFNGRVLKKQELPAGQTKLFDQLVP
                     FFSFAENIVKPPLGQSLILIARKT"
     gene            7908..9311
                     /locus_tag="PLG98_07495"
     CDS             7908..9311
                     /locus_tag="PLG98_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33135.1"
                     /translation="MHFVFVHPPRKIPGGNIWRLINSVNPPLGLALLAALWDREGQTS
                     TIIDAAALQLTIPEIVDQIDPSADFVGITATTPEISSAMTLARDIRNRLPQTRIILGG
                     AHPTVFHRELVKENICDMAVRGEGEEAITKLARNTPLHLIPNLTWRNTQGEIIVNPAA
                     TNYADLDSLPFPAYEKLPMNLYHSALGAAQYQPSIGMVTSRGCPGKCTFCFSEMFGPQ
                     VRFMSPLRVIEHIEVLQKKYGIREISFYDDTFTANKKNVAVLCRLMLEKKINLSWSCF
                     ARVDTVTPDLLQLMKKAGCHQIMYGFETTDEDILKNINKRITAEQFQNAIKWTRAAKM
                     NIRGAFMLGNPGETEASMQRTIAFAKNARIHFAVFNITTPYPGTAMYKEFLRNNSLLH
                     YNWDLYNLAEPVLDLDTVSDNVVRQYYYKSYRDFYLRPIFIFRHIRRIRTLSELLMHI
                     KAVLKIIPLLYRNIFSK"
     gene            9711..11042
                     /locus_tag="PLG98_07500"
     CDS             9711..11042
                     /locus_tag="PLG98_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33136.1"
                     /translation="MNSNGGILYEEMYTRLPYYLSDIPLLQKLFDSQMLDDGLYRARE
                     LSYLFDFIDVKFVELCIEHGYPHFLSLTHYLLCIATGCIIWSFCVKILNLSPLTGIGW
                     LVLFWTSPSVYFSGYFINRTGKVMVMFLTSIIFYFLCKAVVLAKEENSFPISKIDWFL
                     YSISMLIITFLDEQGVFLCMVFFTLLLIWHLIIRQKEFAIMIFVCLATIILHGLYRFF
                     IAPHFIFALNGYRPDFSYQILPMILYVENLAQYLQAGFFLYLDTFRFLIGNPPLPIGF
                     ILLILLIFSPIYFMYANRQRSDNYRKYFGLALGGFLITNVLLVIMNSFMLLKHPPLMW
                     PEARRVFYWLPTTVILVMTLSVWTRLFHKSAVFKFCIVFSICLAVVGNIVALPKHKAI
                     IMRQESYVHSNIQSSSDLLKALKNLYALTDSDDPVIEKNPVFQFFKLKKKD"
     gene            complement(11318..12424)
                     /locus_tag="PLG98_07505"
     CDS             complement(11318..12424)
                     /locus_tag="PLG98_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE33137.1"
                     /translation="MKKIFFKSLLIIALLIVTFVLPVVYSTGIAVYFRLALLFFIVLE
                     SFKLFKIIIIDAKINHALSNFATTLFSFFIIFMILETVFMFVPRTHFFDYTLASKLWY
                     AKYYKPINSLGFRDNEPNYNHNVILFVGDSFTAGSGLKSVDERFSNIVGKELNKKENK
                     YTVINIGHPGADSSDEFHLMQQVIYMTKIRPEIIVLQYFGDDIAHVAAEKLKTFEGFK
                     PFVNVNAFLKPAVRGSYFLNYMYFLFPNKELTSSALSLMQQTYRDNNMLLKHQEQLKL
                     FINYARENHIQLIAVVFPVLEDIEISNSIYVNDIVNYFEVHKITTINVSRLVKNIPLQ
                     ERIINKNDGHPSKSVHASVAHEVLRKIRFNGNNE"
     gene            12524..12700
                     /locus_tag="PLG98_07510"
     CDS             12524..12700
                     /locus_tag="PLG98_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE33138.1"
                     /translation="MKHCRSFAVELKRQLVEELLSGMSTPAQLIRRHEKSSGLLYHWK
                     EQYAKGRFNNEPSK"
     gene            12684..13010
                     /locus_tag="PLG98_07515"
     CDS             12684..13010
                     /locus_tag="PLG98_07515"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOE33139.1"
                     /translation="MNPRSKPPKGVIHHSDRGAQYASHDSVEALLQHGFPISMSGKGN
                     PYDNATTESFFKTLKVEKVYLWEYRTLEDVQIRLPFFIQEFYHRKRLHSSPGYKPPVE
                     SKELFL"
     gene            complement(13242..14675)
                     /locus_tag="PLG98_07520"
     CDS             complement(13242..14675)
                     /locus_tag="PLG98_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33140.1"
                     /translation="MAKVFLINPSMDILSAFGEYHKLMEPMPCIGLAYIASSCQKAGH
                     EVFMLDNFVEKKPFEDIVESVKSFGADIVGISMLTPTANSTEALGHYIKTRLPSVKVV
                     FGNLHASLFAENLINRGACDAVVHGEGEIIFPRLIDAFLNKSDISKIQGISYKGEEGI
                     VFTGMSDPIEDLDALPRPAWELFPWRDYSFLPFVTVAKPCLAISGSRGCPFRCKFCAM
                     GYMGNLVRKRSPESIADEIEWLVKEFGARHIGFVDPIFPLDKKHAIATCRAIRAKNMP
                     ENWWWTCETRVDIVDEEMCREMKLARCKRILFGIESGVNEVLAGVGKGYTTDNVKRGI
                     VAARSAGLEISAFFMLGLPGETKETAKQTIDFALSLDIDFAKFAITIPLPGSELYDDL
                     VKQGKISQPEWESFTTFNPDPDSLPYIPEGMTGKELLQLHRMANVKFYLRPKMIFRHL
                     FVIRSIGLKMMFNGAMLILKQLFKDRK"
     gene            complement(14678..15430)
                     /locus_tag="PLG98_07525"
     CDS             complement(14678..15430)
                     /locus_tag="PLG98_07525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33141.1"
                     /translation="MDFLNPVSSFYHLKSLLTTLYLHRLENYLDPIPRESIVLDAGCG
                     VGRFTTLLAERFKKVVAFDPSLPSLKACQRHLEERGLHNVEMHWADISFLDNVGENTF
                     DVVIATGPICYTADSLKSLKRLMRVAKPNAKIFLSVEGCLGALCSQGVEGPERLLNVL
                     SRDPLLLEKDRLINYCNRKDFKELVRNAGVKKLTIDSSHDFGDGAFWQAIDDSRLNDP
                     TYVDMIIRTEDLCRNHSVIAERARIFMAVGEK"
     gene            16146..16754
                     /locus_tag="PLG98_07530"
     CDS             16146..16754
                     /locus_tag="PLG98_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33142.1"
                     /translation="MNWDFHNILFYPPITGTTILSVDLPPHFRVIAYLIAGIVLLLGT
                     SFFFAKKYSLLNAFRKAIIIAFFSSALFYAIYTDIGWSTWLKTDIKKYWGLSTEEKSI
                     KMYNGLYVFSMEAKKLIHDDYIIYSSYFQEPRLFQYYLLPLNKRSDNHYRLEKAPYIV
                     VLKDTESQYDLAERVFTRGEITIKNVELISMFAHDAYILKRL"
     gene            16751..18208
                     /locus_tag="PLG98_07535"
     CDS             16751..18208
                     /locus_tag="PLG98_07535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE33143.1"
                     /translation="MMLSTAIAIFLPTLLGFLIITIILRNDKETFFGERIGLSFPLGA
                     GILTLQIFLLGLMRIPLTLFNSLMPALFELMLLSFWIWKNKIVLIPSISNPGLSLFLE
                     IKSPQNHGLKKCLFALLIIWIIAKLVSVFILTGLRPIFAWDAWANWSAGAKIFFYSKS
                     LLLDAPALEFFGGNAVDRILYYPLHNTLLQLWMSLWMGKFDDVFVKFFSPVYCLSMII
                     CLYYIAIREIGKLYALALLAIFLSSPLLSYHSIEMYSDQMLGVYLLFASLSFLKAIRQ
                     NLSFCILAGAYATIAVFTKNEALFFVLPFLLSAIVYFRHDLNKSRGKYVNLISILAPF
                     LALLPWFLFKVHYGLGFLGQLRWLRWSHELYFEYYAHSPDTSLWSFFQPDMILVYIYK
                     MISLDNFNVIFLFFPLIILAHGKISKELLHLLVPIAGYMLFFLFLYIFTAYYMGQSMC
                     LTRNILTFYPLFFFLTILLLKKDDTVLSYPAPITR"
     gene            complement(18251..19591)
                     /locus_tag="PLG98_07540"
     CDS             complement(18251..19591)
                     /locus_tag="PLG98_07540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE33144.1"
                     /translation="MTENNRLKGKIASIPDYAFLGVILVVCLLYRIYYFGFLNPGMVQ
                     YNSDSVSYFYAVDLFRGGVDLYRTPLYPYVIKFFQYVSGDHLVRNLIFFQQALSFLSI
                     IPFYFVSKRVIGNVYVGMIATLFYGCWHPILIQNVHLNPESLCFAGSVFMLYLLVRFL
                     EKPKRSAALAIGIFPFFLIMLKPTYLILIAVMLLFLLCRFFLLRDERKILYWGLLGLC
                     FTAAGVLGYCGMNKKFNGQFVLSNIALNNTIAHLSISGAYEYGKDEEFMAVIDRTRHI
                     NYYAAPFSINNHFIDNYKRCYRNFPKDLPPTEDMIFCSVFPDRVNYSPERIIRFISHA
                     RCTVVYVKYMAQRAFDIIVAAYGNLFLLLALQSVLIMVAAVKYKKIAWAQSFCIVFVL
                     GQFFSIWLAGLDDMNRHLMPSYPFLIQIAGSFAVNIGLFLSKNKLSSKSDNAAF"
     gene            19820..>20482
                     /locus_tag="PLG98_07545"
     CDS             19820..>20482
                     /locus_tag="PLG98_07545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE33145.1"
                     /translation="MAHRLNGKNTIITGAANGIGRASAVLFAREGANLVLADISETGL
                     RQVEDEIKGMGGNVVIRKTDVASEEEIQALINLALEKFNTIDILVNNAGIGGGLDALE
                     DQKPEEWHLVYDINVMGPVYATKHIIAHMKERRRGSIINIASVAGLLSGAGGNAYSAS
                     KAALINFTKTSACEVGAFNVRINAVCPGLIETGMTQPFFDWARSTGKENQLGKYCELK
                     RAA"
BASE COUNT         5849 a         4804 c         4175 g         5454 t
ORIGIN      
        1 cctgaaaatg gcggcgcggg atattggttt catgttctcc cgccgggtct ttcacaagga
       61 cctgcaactg aataatattc tcctgaaccc tgccgatcct gcacaactgc actggataga
      121 taatgatctg aaaaaaatcg gcaggcgtcg ttgggccaaa caaccgaaag ccgtccttga
      181 acaaataaca aggaatgttc agttttgttc gattgatgaa aaaaatttct ttataaagga
      241 aattgtttca tcatgggcaa tccaaaaaga agaggatgac ggacagccgt cctgaaaaac
      301 agccatcgtt catttttgat catgaggcgt tacggtaatc accttttggg cattcctgaa
      361 cttttcccgc gacaccattt tcctgaggcc ggcgatcgtt ttgatgccgt aatcgctggt
      421 cagcatgttg accagccagg aaggcaattc acctccggga tcgccgagga cctgatagat
      481 cacttccgtc ttatcctcac ctttcggcgc cagcgtgcat tcaatgatca aaagcggcat
      541 ccgcacgata tccttatttt gcctgaatgt tgttttcagt cctttcgcac ggcaatacac
      601 ggtccccgtt ttcctatcga cagaccatac ggatttgacg acagcgtcac ggtcgctgac
      661 ggggaaaggc atgtcggtga cgctgtggaa atagacggca tctcctgcac ttttcatctc
      721 cggggtattg atcagggccg cctctttgca taaaaacata tactgcggca tgatggaaat
      781 atcgcggata acggcctcga ccaccgcgac cggagcattc acaacaccga ccgcccttat
      841 ttcatcaaca gaactcttcg ctgtcggacg cgtaaaaccc gcaatgccgt ccgattctcc
      901 gactttcttc cagccgtcgt cggctgcaga ccacgccggc ccggtactcg ccgccaccat
      961 caaaatcgcc agtacaaacc gtctgcaaaa tgctgtcata gttatttccg cttcacgcaa
     1021 tcattctttc ccgccattct atccaagaat tctataggct tttaatcttc tgagcaaata
     1081 aaatatctgt ctgtaatcca ttaaggaaag aagtctgaac aagattcgcg gtctcaaata
     1141 aaatttccgg aaggccctgg attgcgcctg catcaattgt tctttggtca ttccctccgg
     1201 aagatattcc ggttggcgaa aactctcttt tgaccagttc ggcgtgaatt ttccccggag
     1261 gctatcccac aattccgacc cgggcagaac atccaggatg acgaattgcg ctcttgacaa
     1321 tcccgacctt aaggcaaaac ggatattgtc ttccatcgag gccgccgttt ctcccggcag
     1381 cccgaaaatg aaaaaaccct gcgtggaaat gccggctttg ccggtcatat cgatggcgnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aaatgaaaaa accctgcgtg
     1561 gaaatgccgg ctttgccggt catatcgatg gcgtgccgga tggtttcgac cgactccctt
     1621 ttcttgatat ttttcagaat ggacgaatga acggattcga tgccgtaagc gatgtaatag
     1681 catccgcttt gcttcatcaa agcaataaga tcctccgaaa tattatcggc acggacgccg
     1741 ttcgggcagg cccagtgtat cttgatgttt ctttccgtca tcaggcgaca aagctttttc
     1801 acgttatcgg cgttcagcgt gaaattatca tcttcgaaat gaacttcact gatgttgaag
     1861 tttttcatca gatactcgat ttcggcaacg acattttccg gcgttctgaa tctgatggtt
     1921 ctgtcgcaga atcggggagc ggcgcaaaag gtgcactcaa agggacaccc ccggctggtg
     1981 accataacac ctacgggaaa gccccggaca accgcgccgt gcggggcgcg gggatattcc
     2041 cgcggatcaa tctgttccca atcggggaaa ggcagatcat cgagattgtc caccacggac
     2101 gctttgatga tctcctgctg ttcgcttttg aggttttcca gcgaatacac tccccggata
     2161 ctcttgttgc aaaatttact ttcggccaat tcaagcagcg gaatttcccc ttcgccgcaa
     2221 acgacaaaat cggcctgcga ctccaacagc gtgcggtacg gcagaaacgt aggatgctga
     2281 ccgccgatga cggttgtgat tttatttttt gcaagttcgc gggccaacac gaccacttct
     2341 tgataaaacg cggtgagaca ggtaatgccc accgcatcgg gattgaattt ctgaatccgg
     2401 cgcatcagcc gcgacgggct gagattctct ttcagaccat caataatttt tgtctcgatg
     2461 ccggactttt tgagatacga cgagagatac cccaggccga tcggcggtgt tatgatatgg
     2521 cttttatagt ttggtcggac cagggctatt ttcatgtcac aagtttcaga tgtcttagtt
     2581 tatataattc tatgatcagt tttattccca acccgccaaa ccgaaacaga gcaatcgacg
     2641 attggccggc attgcgcggg ttgaaagaaa tttcctgcca taagattttt tctccctccc
     2701 gggtcagcat cgcggacaga agcacattgg gaataaagaa attgtcggga atcagcggca
     2761 ggtacaggcg caaacgttca gccttcatta aacggaaagg ggcgttggca tcctttaacg
     2821 aaacattgaa cagaagcgcc agcactcctt tcaggatccg cgaaatgaaa accctggccg
     2881 ggccatccgc ccgtcttgcg cgccaaccga taataaaatc gcaggtgtcc cgtttttccc
     2941 agaacctcca gaattcgccg gattgcgtct gaccgtcggc atcggtttga aaaacccaat
     3001 ccgctccttc agcgacggca aatttatagg cagccagaca cgtcggcccg tggccggtat
     3061 tggttttgtt gataaccgtc aggtgcggat ggctcgtttg gatactctcc agaatggcag
     3121 acgtctcgtc cgtgctgccg tcgttgaata cgacaagttt gctgccgtgg ccgacagaat
     3181 cgacaaccgc atgccattcc tgaacgactt tttctattat ccccgcttcg ttgtaagccg
     3241 gcatgacaac atagagctta ttcataatcg tcctgttttc ataagatcaa aaatccatta
     3301 cggcatgtgc agcatttttt taaatgacgg atccaccgtg cgtcccgtca actccgccat
     3361 ccgcacatcc tgagcagcct gttccatgcg accgagccgg taaaaaacga aagccctgtt
     3421 taaatagttg ttcggattat acggattgag ttttacggcc gtgttgaaat cagcgatcgc
     3481 tttttcaaac tgattcatcc tggaaaaaat caccccccgg ttgcccagaa cgttggacag
     3541 atgaacatcc ctctcgctcc cgccggcatc gtagggccct ctcagcgcca aatagcggtt
     3601 gtaatcctga agggctttgt taaaatcatc cgatatcaga taggcaaggc ctctgttctg
     3661 atatgccaga agactctgcg gattgagatc aatcgccgcc gtatagtcct ggatagcatc
     3721 attgtaccgg ttttgcacac accggtcatt accacggttg aagtaagcca tggatatttt
     3781 acggggatat ttttctataa cgtccgtcca gagtgtttcg ctgtttttcc agaccggaat
     3841 atatttaatc gtcaggacgc tgaagatcag cagaaccacc acagcggtcg ctgccacggg
     3901 caggcggcgg ataggatacc gctcttccat tttctgcatg gaaaaggcag cggcaaacgc
     3961 cagtccaacg caggccatgt agatataccg atcgttcaga taggccgtat cgttcgccag
     4021 aaatggcatc agaaggaaaa gggtgttgaa tgtaaaaaaa agcagcccga atgtaacaaa
     4081 ccggtattgg aaacggacag ccagggccca gagaaaaatc aaaacggctg taatcgcacc
     4141 gatccagtgg cccgcatgca gagccggggg aacagggtac agcgtggatg tcacataggg
     4201 aataaaaaac ttcagaatat agattgtata agcataaccc gccagcaccg tctgttcaaa
     4261 gaaattaaaa gcattgacga tcgccttgct gtctatggtt ttataaacat tttgaaagaa
     4321 aaacgttgtc cccagcaaac caaccagcaa agccatggcg aagaaaatta atttttccat
     4381 taccgctttc ccgttcatct ttcttccgaa ataccagtcc agcaaaacca gcgagaaggg
     4441 aagcgcaacc gcctgcccct tggaaagcag cgacaaaaaa aataagcaaa aggttagcaa
     4501 aaaataacct gctttgccgg aagcaatgta acgaatataa gcgagcaaag ccgacaaata
     4561 aaaaaatcca tagaggacat ctttacgttc ggtgatccat gcgacggact ccacccgcat
     4621 cggatgaatg ccgaaaagca aggcaccgac accgctccac cagacagaga gacccatcct
     4681 gcggaaaaga agcagaacga gccccgtaca aagaagatgg agaatcagat tgtcgaagtg
     4741 ataaaggaaa ggtctgtctc cgaccagttt ccattccaaa gcgaaagaag aaataaccag
     4801 aggattatac gtgttatagt cggccggctt ggtgaatatg tcgatgaacc gtgcttcacg
     4861 aaccagggaa ttttcgtaaa ccaatagatt gtcgtcccag ttggtccaat ccagtctcag
     4921 gctgccggaa aaaacaagcg cagtcacggc aaggactccc aaaaatataa gacatttgcg
     4981 cttttccatt ttacagcgat ccccatttat cccggatgaa ctcgatatcg ttagacttat
     5041 aacatatgcg caaggacatc acctgtcaaa gtaacatttt tttaaagtgc aataacttat
     5101 ttttcttctt tatccggata gatttcatga tcagtcgtca ggcgctcagg tgcaaaaata
     5161 aagaaataac cgcctcccat ccacggaatt tcagcaggcc acggtttggc aaataccgtc
     5221 acatatccgg gaatctcaag ttttttaaat gacagaatga ccgctttttt ctcttcaatg
     5281 gattgcacct gccgggatat atcatcgacg ccgggaatgg gtacgggaga gatctggacc
     5341 ctgggcaaat cattcattac aaacgcaacc acatctcttg tgtaattgac gatataacaa
     5401 cattttttat agggttttat aacatcaaac acttcttcgt aaaagctgtt gtattctttt
     5461 gttaaaattt tacctttcag tatcgggacg ctgctgtttt ggatacctcg gccgaagaga
     5521 acgtcccttt gccagggata atacgctgat gtcgtctgtt tgaaaaacaa cgttgacagc
     5581 aataccgccg caacgattaa tcccgctgcc ggaatcagat atttgagcgg ttttccgacc
     5641 ttatcaaaac aataaccagc cgccagcaca cagacgccga tgcccagaga agcgccgttc
     5701 accaggcgga atgtttcata aacatggatg gaattaagat aaccgaagac ggcgatcaga
     5761 caagcggcaa acagagcacc gtctttatat gccggctcgc gaagcctgct gaagacgtac
     5821 tgacgaattt ctttgatcgc aaaaaacaga caaaccagca acaccggcgt aaatattttt
     5881 ccgcgaagat ccgaggccag actgttttcc atctggagca ggaaaatcag aaagctcaaa
     5941 tacgtattga cattatctcc ctcaccgatg aacctgacaa tcgttgtatt ttgaagggaa
     6001 aaatcatcca acgcggaatg cagaaaaaga tatgcaaaaa aagccagcaa ggggatgaaa
     6061 aacccggcgc tgatcaatcc gattttttcc atcacgaatt tctttttctc cggggtaacg
     6121 aaatattccg cacaaagcaa aacggcaaaa ggcggaacaa cggcaataac ggacgaatac
     6181 cgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcacaa agcaaaacgg
     6301 caaaaggcgg aacaacggca ataacggacg aataccgcgc cagaaccgac atgcttaaaa
     6361 aaaatcccgc cagcaggccg taatatttgc tttcggaata tttcagaaaa tagacaagcg
     6421 ccagcaattc aaacatatac ataaaataat taggcgcggg ataaatggca taagggtgaa
     6481 tcaggaaaat cagcagcacg gaaaggcagg cgagacccct cttcatcaat ctgagaaaaa
     6541 cacgataaga aaggaataag gacatggaat aaaacaggcc cgtgacaatg cccagactca
     6601 ccagcctctc accgaacaac atcaacgaaa tgctctgaat ccatgtataa atatagccgt
     6661 aaaagataat gacttcggaa tgcgggatgg ctcctctttt caaatccagc gcggcaatgt
     6721 aggcccagcc ccagtgcaaa ttgtccacgt tcactctggc ttctatgata ctgcagacaa
     6781 aactgatcag gcatatgcca agaaccagaa aatcaaaccc gttgaatgcg tcaaaagaga
     6841 aaaacccttt attgacaccc tggggatggc ctgatttatt ttcgtgaacg gataataccg
     6901 aaacgctcat catggctttt gaggcaacct cttcttaggg ttcggttttg tcccgggata
     6961 caaagagaga tgtttatcag accgttcaat tccggcttga caaatcacca ttcccgttgt
     7021 tttttaaaag aaattttaaa acgtttaaat tatttaattt ttcgcagacg attgcaacat
     7081 ttttttagat gacctttatc catgaataag gtaatgaaga atcatccact taaaaacggg
     7141 gaatgaacat caataattca aatctccata atgaaaaaat aaagtattca ttgctgctgt
     7201 tgtcccaacg atataactac aatcgctgga tatacaacaa catacaacgt tttatcggaa
     7261 ataacgttct ggaagtcggt gcgggaatcg gcaatctcac ggactttatc ctttccaaaa
     7321 acaaacttac gttgatcgac atccatcagc cctatgtgga ttatttaaag gcgaaatact
     7381 ctttttgcga cagcgcggcc ttcacggtcc tccaaacgga tattcaaaac attcaatcat
     7441 cgcccgtctc cggcatgacg ttcgacaccg tgatttgcct gaatattctc gagcacctcg
     7501 aaaacgaccg gacggccgtt gaaaacatga gtcatctttt acagccgaaa gggaaactca
     7561 tcatcctggt ccccgccctg aagacgcttt acggctcgat ggatatttct tttgatcacc
     7621 ggcggcgcta taataaaaaa gatttaagat cgctcatcca ggatcaaaat ctggacgtgt
     7681 tgaaacttta ctatatgaac tttccgggca tgctcggctg gttctttaac ggacgtgtac
     7741 tgaaaaaaca ggaactgccg gcgggacaga ccaaattatt tgatcaactg gtgccgtttt
     7801 tcagctttgc ggaaaacata gtcaagccgc cgctgggaca gtcgctgatt cttattgccc
     7861 ggaaaacgtg atttgcacgc aataaaacaa ctccgatgat tcgacctatg cattttgtat
     7921 ttgttcatcc gccgcgaaaa ataccgggcg gcaatatctg gcgtctgatc aattccgtca
     7981 atccgcctct cggattggcg ttgctggccg cgctatggga cagggagggg caaacgtcga
     8041 caatcatcga cgcagcggct ttgcagttga ccattccgga aatcgtcgat cagatagatc
     8101 cctcggcgga ttttgtcggc atcacggcaa ccacgcctga aatatcgtct gccatgacgc
     8161 tcgcccggga catccgcaac agactgcctc aaaccagaat catcctgggc ggcgcacatc
     8221 cgactgtttt tcatcgggaa ctcgtcaaag agaatatctg cgatatggcg gtgcgcggcg
     8281 aaggcgagga ggcgataaca aaactggccc gaaacacacc gttgcatctt attcccaacc
     8341 tgacctggcg aaatacgcag ggcgaaatca tcgtcaatcc agcggcgaca aattatgcgg
     8401 atctggacag cctgcctttt cccgcttatg aaaaacttcc gatgaacctc tatcattcag
     8461 cgctgggggc ggctcaatat caaccttcca tcggcatggt cacatcccgt ggctgccccg
     8521 gaaaatgcac attctgcttt tccgaaatgt tcggtccgca agtcagattt atgtcgccat
     8581 tgcgcgttat tgaacatatc gaagtgttgc agaaaaaata cggcatcaga gagatttcct
     8641 tctatgacga cacattcacc gcaaataaaa aaaatgtcgc cgtattgtgc cggttgatgc
     8701 tggagaaaaa aatcaatctc agctggtcat gttttgcacg cgtggatacc gtcacaccgg
     8761 acttgctgca gttgatgaaa aaagcgggct gccatcaaat catgtatggt tttgaaacca
     8821 cggacgaaga tattctaaaa aacatcaaca agcgaatcac cgcggagcaa tttcagaacg
     8881 ccatcaaatg gacaagagcg gcaaagatga acatccgcgg cgcttttatg ctcggcaatc
     8941 cgggagaaac ggaagccagc atgcaaagaa ctatcgcttt tgctaaaaac gcacgaattc
     9001 attttgccgt tttcaatatc acgactccat atccgggcac cgccatgtat aaagaattct
     9061 tacgcaataa ttcccttcta cattataatt gggatctgta taatctggca gaacccgtgt
     9121 tggacttaga caccgtatcg gacaacgtcg tcagacaata ttattataaa agctaccgtg
     9181 atttctatct gcggccgatc tttatttttc ggcatatccg caggatacgc actctttccg
     9241 aactgttgat gcacattaaa gcagtactaa aaataattcc ccttctctat agaaatattt
     9301 tttcaaaata aatttcaggt tataaattaa aaatcttttc tgcaaaaaaa cagcagcgaa
     9361 cattccggaa aagtcttgct gatattcccg gaagccaaag cagctataca gttcaaccat
     9421 aatttcattg acaaaaaacg gcattctccc tatatattca caacactcaa ctggaaataa
     9481 cactctatca caaagcatca tcagccaggc aatgcgccat gggacagata accgatatgc
     9541 ccgggaccga attagttttt taaccgctgt attgaaacat caagcacagc cgtcggaaca
     9601 tttatttcat gaaattatgt aaacttataa aatttccgga tcattttaag gttgacatct
     9661 cttgctttca cgcgtttttg ttaagttgtc tgattgcact gatttccatc atgaactcta
     9721 atggcggtat tctgtatgaa gaaatgtata ctcgtctgcc ctattatttg tcagatattc
     9781 cattattgca gaagttgttc gacagtcaaa tgctggatga cggtttatac cgtgcacggg
     9841 aattaagcta tttatttgac tttatcgacg ttaaatttgt tgaactctgc atagagcacg
     9901 gttacccgca ttttttatct ttaacacatt atcttctgtg tatcgcaaca ggttgtataa
     9961 tttggtcctt ttgcgtgaaa atattaaacc tcagtccttt aacagggatc ggctggcttg
    10021 tactgttttg gacttctcca agcgtctatt tcagcggata ttttattaac aggacaggaa
    10081 aagttatggt catgtttctg acgtcaatta tattttattt cctgtgtaaa gcggttgttc
    10141 ttgcaaagga agaaaatagc tttcctatct caaaaattga ttggtttctg tattccatat
    10201 caatgctgat cataacgttt ctggatgagc agggagtatt cctctgtatg gtgtttttta
    10261 cactcctgct tatatggcac ctgattatcc gccagaagga atttgccatt atgattttcg
    10321 tttgtctggc aaccattatt cttcacggat tgtatcgatt ttttatcgct ccgcacttca
    10381 tatttgctct caacggatac cggccggatt tttcttatca gatattgccg atgatactat
    10441 atgtcgaaaa tttagcgcag tatctgcagg cgggattctt tttatatctt gacacattcc
    10501 gatttttaat cggcaatcct cccctgccca tcggctttat tctcctcata ttgcttattt
    10561 tttctccaat ctatttcatg tacgctaacc gtcagaggtc cgacaactat cgaaagtatt
    10621 tcggtctggc gctgggagga tttttaataa cgaatgtatt gctggtcatc atgaattcat
    10681 tcatgctgct aaaacatccg ccactaatgt ggccggaagc cagaagagta ttctattggt
    10741 tgccgacaac agttattctt gtaatgacac tttctgtgtg gacccgtctt ttccataagt
    10801 ccgctgtttt caaattttgc atcgtttttt caatctgtct tgccgttgtt ggaaatattg
    10861 tggcgcttcc caaacacaag gccattatca tgaggcaaga aagttacgtt cactctaaca
    10921 ttcaatcaag ctcagattta ttaaaggcac ttaaaaacct ttacgcactg acggattccg
    10981 atgatcctgt gattgaaaaa aatcctgttt ttcaattttt taagcttaaa aagaaagact
    11041 agcggaataa ccttatcatg cacaatggaa ggggagaagc ctgcgatacg agacaatatc
    11101 cccagagata agcagacatg gaaagtttga tctgaaagtt ccgatgtgaa aatgacgtct
    11161 tttcgtcaag aggattgttg attaaaaaag gttttagata aaagaagaat ttatcaattc
    11221 ttcttaaaca tgatcaatag tcttttcaga acaaaaccat ggctatcggc agcaatttat
    11281 tttgttctcc aacaaaaatc ggatcatcgt aaaatattta ttcattattt ccattgaatc
    11341 taatctttct caaaacttca tgagcaacgg atgcatgaac tgattttgaa ggatgcccgt
    11401 catttttatt tattattctt tcttgcaggg gaatattttt aaccaatcga gagacgttga
    11461 tagtagttat tttatgaact tcaaaataat tcacgatatc attgacatat atagaattgc
    11521 tgatttcaat atcctcgagg acaggaaaca ctaccgcgat aagttgtata tgattttctc
    11581 gagcgtaatt gatgaataat ttaagctgct cctggtgctt taataacata ttattatccc
    11641 gatatgtttg ttgcattaaa gaaagagctg aagacgtaag ttctttattg gggaaaagaa
    11701 aatacatgta attaagaaaa taagagcctc ttaccgcagg ctttaggaat gcattcacat
    11761 ttacgaaagg tttaaaacct tcaaacgttt ttaatttttc cgccgcaaca tgcgcaatat
    11821 catcgccgaa gtattggagc actatgattt cgggcctaat ctttgtcata taaatgactt
    11881 gttgcatcaa atgaaactca tcagatgaat ccgctcccgg gtgtcctata tttattacag
    11941 tatatttatt ttctttttta tttaattctt tgccaacaat attggaaaat ctttcatcta
    12001 cagatttcaa tccactgccc gccgtaaatg aatcgcctac aaataaaatt acgttatgat
    12061 tgtaattagg ctcgttgtct cgaaaaccta aagagttgat cggtttataa tatttggcat
    12121 accacaattt ggacgctaaa gtataatcaa agaaatgcgt tcgtggaaca aacatgaaga
    12181 cagtttccag gatcatgaaa ataataaaaa atgaaaataa agttgtggca aaattcgaca
    12241 atgcatgatt gatctttgca tcgatgataa taattttgaa caatttgaaa ctttcaagga
    12301 ctataaaaaa cagcagagct aatctgaagt aaacagcaat accggtagaa taaaccacag
    12361 gcaaaacaaa agtaactatc aacagggcaa ttatcaacaa acttttaaaa aatatttttt
    12421 tcatagtaac aaattagact gcccccccac ccggattgaa caaaatatat tagactagtt
    12481 ggcaggaacc agattccctt tatgggaaag aaaggaactt gccatgaaac actgcagatc
    12541 atttgctgtt gaactcaagc gccagctcgt tgaagaactt ttaagcggta tgagtacacc
    12601 agcgcaactg attcgccgtc atgagaaatc atccggcctt ctttatcact ggaaagagca
    12661 atatgccaaa ggacggttta acaatgaacc ctcgaagtaa gccgcccaag ggtgttattc
    12721 atcattcgga tcggggcgcc cagtatgcct cccatgattc cgtagaagcg ctcttgcaac
    12781 atggctttcc gatcagcatg tcgggcaaag ggaatcccta cgataatgct accacggaaa
    12841 gctttttcaa aacgctcaag gtcgaaaaag tttacctttg ggaataccga accctggaag
    12901 atgttcagat tcggcttccc tttttcatcc aagaatttta tcaccgcaaa cgtctgcatt
    12961 cctctcctgg atacaaaccg ccggtggaat ctaaagaact ttttttataa aaaccaaaaa
    13021 cccgcgccga ttgctctaac ccgatctatc taaccgcagg ggctcaatcc agagactctt
    13081 ccaacctatc ttgagcggca tgcaatccgg aaggataacc tcatgccctt gccgtctggc
    13141 ctccgatcca agccaggcaa gcgcctgaag aagcagataa tcatcctgct cattcatgta
    13201 atgccatata tatttcggtg gccgaacaaa taataatcgc attacttcct gtctttgaac
    13261 agttgcttca atatcaacat cgctccatta aacatcattt tcaaaccgat tgaacgtatc
    13321 acaaaaagat gccggaatat catctttgga cgcagataaa actttacgtt tgccatccga
    13381 tgcagttgca agagctcttt tcccgtcatg ccctcaggaa tatagggaag cgagtccgga
    13441 tcgggattga aggttgtaaa gctttcccat tccggttgtg atattttccc ctgcttcacc
    13501 aaatcatcat agagttccga accgggaagc ggtatcgtta tcgcgaactt tgcaaaatca
    13561 atgtccagcg atagcgcaaa atctatcgtc tgttttgccg tttcttttgt ctcgcccgga
    13621 agcccgagca taaaaaaagc ggagatttcg agtcccgcag accttgcagc aacgatcccc
    13681 cgcttcacat tatccgttgt atacccttta cccacaccgg ctaaaacttc attaactccg
    13741 ctttcaatgc cgaacaagat tcgcttacat cgcgccagtt tcatctcccg gcacatctcc
    13801 tcatcgacaa tgtccactcg tgtctcgcat gtccaccacc aattttcagg catgtttttt
    13861 gccctgatgg cccgacatgt tgcaatcgcg tgttttttat caagtggaaa aatcggatca
    13921 acaaatccga tatgtcgggc accgaattcc ttcacgagcc attcgatctc atcggcaatc
    13981 gattccggac tgcgttttct gaccagattg cccatataac ccatcgcgca gaacttacac
    14041 cggaacggac agcccctgct tcccgaaatc gccaaacagg gcttggcgac tgtaacaaac
    14101 ggcaaaaaag aataatctct ccagggaaac agttcccaag cggggcgggg aagagcgtct
    14161 aaatcttcta taggatcgga catccctgta aaaacgattc cttcttcgcc cttataggat
    14221 atcccctgaa tttttgaaat atcgctcttg tttaaaaatg catcgatcaa ccttgggaaa
    14281 ataatctccc cctcgccatg cacgaccgca tcgcatgctc cccgattgat aagattttca
    14341 gcaaatagcg acgcatgtaa atttccaaaa acaactttga cagacggcag ccttgtttta
    14401 atgtaatgtc ctaaagcttc tgttgaattt gcggttggcg tcaacatact tattccgacg
    14461 atatcagcgc caaaagattt gacagattca acaatgtctt caaaaggttt cttttccaca
    14521 aaattgtcga gcatgaatac ttcatgaccg gctttttgac agctcgaagc gatgtacgcc
    14581 aaaccgatac acggcatggg ctccattaac ttgtgatact ctccaaaagc ggacagaatg
    14641 tccatagaag ggtttattaa aaagacttta gccatcttca cttttcccca acagccataa
    14701 aaatcctggc ccgttcggca atcacggaat gattccggca aagatcctcg gttcgaataa
    14761 tcatatctac atatgttgga tcattcaggc gcgagtcgtc aatggcttgc cagaatgccc
    14821 catccccaaa atcgtgcgaa ctgtctatgg taagcttctt cacacccgca ttccggacga
    14881 gttctttaaa atcttttcgg ttacaataat tgatcaaacg gtccttttca agcagaagcg
    14941 gatctctcga aagaacgttc agaagccttt ctggcccttc aacgccctgc gagcataatg
    15001 cacccaggca tccttcaacg gataaaaata tttttgcgtt tggtttcgca acgcgcatca
    15061 aacgcttaag ggattttaga gaatcggccg tgtaacaaat gggtcccgtc gctatcacga
    15121 cgtcaaaagt attttccccc acgttgtcca aaaacgaaat atccgcccag tgcatctcaa
    15181 cattatgaag cccgcgctct tccagatgac gctgacatgc tttcaacgac ggcaatgaag
    15241 ggtcaaacgc aacaaccttc ttgaatcgtt cagccaaaag ggtggtaaat cttccaacac
    15301 cgcaaccagc gtcaagaact atagattccc ttggtattgg atcaaggtaa ttttcaaggc
    15361 gatgaagata cagcgtggtc aaaagactct tgaggtgata aaaagaagag accggattga
    15421 ggaaatccat atcaaaggat tgatttttga aatatcgttc caatcctaaa acactgtgca
    15481 gtcccggccc gatagctgat gcccgttttt tatctttgtc tttcccgtat aacagaggaa
    15541 ctattccctc agcctgccaa gccttttttg tcaatatcaa tgctccttat ccgtttccaa
    15601 gagctcgttt tgtcacactt tacaacaata aatcatagga gatgaaataa tcaatgtgca
    15661 aaggcaattt tataagaaag gttttaacaa tacttggacg tcttgcgctt ttccctgtta
    15721 aatcaacagg agacacagcg ccctccgttc ttttttaaaa aaaagatttc tccccaccta
    15781 gagacgtaaa acaagggcac aactacatta atggggtttc ggcctcggct tgggagaggg
    15841 tctccacacc tgtattttcc ccctccttat ggttttattt ctatataaaa ataaaggccg
    15901 agtcaataca tattatgaga aacattagcc attttcatca tcatctgcaa ccaaaagcaa
    15961 aacttaaaaa atcacagagg ggaaatgaca taatatcaag ctttttttac acatagaaac
    16021 tattccgggc acctatcatt ttcttcaaac aagcgctcaa tgtatgagtg ttgtattgat
    16081 ttaaaaacca aaaggaattc cttactattg acttatattg gttttttgct ataaaaacaa
    16141 aaactatgaa ttgggatttt cataatattc ttttctaccc gccgataacc ggaaccacta
    16201 ttttatcggt tgatctgccg ccgcatttca gagtaattgc atacttgatt gcaggtattg
    16261 tcttactcct gggaacatca tttttttttg caaaaaaata ttctctgctt aacgctttca
    16321 gaaaagctat cattatcgcg tttttctcct ctgcgctttt ctacgcaatt tatacggaca
    16381 tagggtggag tacatggctg aaaaccgata ttaagaagta ctggggtctc tcaacagaag
    16441 agaagtctat taaaatgtat aacggcttgt atgtattctc tatggaagca aaaaagctga
    16501 tccatgatga ctacataata tattcttcct attttcaaga accgagactt tttcaatatt
    16561 atctactgcc gctgaacaaa cgttcggata atcactaccg gcttgaaaag gcaccgtaca
    16621 ttgtcgtact taaagacacc gaatcccaat atgatcttgc tgaacgcgtt ttcacgcgag
    16681 gtgaaatcac catcaaaaac gtagagttaa tttcaatgtt cgcacatgac gcttatatac
    16741 taaaaagatt atgatgctat ccacagccat cgccatattt ttaccaacct tacttggttt
    16801 tctcattatc accataatcc tcagaaacga taaagaaact ttctttggcg aacgcatcgg
    16861 cctttctttc cctcttggcg ccggcatttt gacactgcaa atatttttgt tgggactcat
    16921 gcggattcca ctaacattat ttaattcctt aatgcctgcg cttttcgaac tgatgttact
    16981 ttcattctgg atatggaaaa ataagattgt tttaattccc tcgatttcaa acccaggttt
    17041 gagtttattt ctggagatca agtcccctca aaaccatggg ttaaagaaat gtttatttgc
    17101 tctgcttatc atatggatca ttgcaaaact tgtttctgta tttattttaa caggactcag
    17161 accgattttt gcctgggatg catgggcaaa ctggtctgcg ggagcaaaaa tcttcttcta
    17221 ctcaaagagc ttgctgcttg atgctcctgc actggaattc tttggcggta atgccgtgga
    17281 tagaatcctg tactatccac tgcacaacac cttactgcag ttatggatga gcctttggat
    17341 gggaaagttt gatgatgtat ttgtgaagtt cttcagcccg gtttattgtc tcagtatgat
    17401 tatctgttta tattacatcg ccattcgtga aatcggcaag ctctatgccc ttgcactgct
    17461 cgccatcttt ctgagttcac cgcttttgtc ctatcattcc atcgagatgt atagcgatca
    17521 aatgctcggg gtttatttgt tgttcgcatc cttatctttt ttgaaagcca taaggcaaaa
    17581 cctctctttc tgtatactgg ccggagcata cgcaacgata gctgttttta ctaaaaacga
    17641 ggccttgttc tttgttttgc ccttcctttt atcggccatc gtatatttta ggcatgattt
    17701 aaataaaagc cgcggaaaat atgtcaacct gatttcaatt ttagcacctt ttttagcact
    17761 acttccctgg tttttattta aagtccatta tgggctgggg tttttgggac agttgcgatg
    17821 gttgcgatgg tcacatgaat tatatttcga atattatgcg cattcacctg atacctcatt
    17881 gtggtctttt tttcagccgg acatgatttt agtttatata tacaaaatga tatcgctgga
    17941 caactttaat gtcattttcc ttttctttcc gttaattatt ctggcacatg gaaaaatatc
    18001 aaaagagctt ttgcatcttc ttgttcccat tgcaggttat atgcttttct ttctttttct
    18061 ctacatcttt accgcatatt atatgggaca atcgatgtgt ctcacccgca acatactgac
    18121 attttacccg ctcttctttt ttctaaccat attgcttctt aagaaggatg acactgtact
    18181 ttcttatccg gcacccataa caagataata ccgtgtcgtc attgtgattc acgggaaatt
    18241 ctttcgtata tcaaaatgcg gcattatcag atttactcga taatttattt ttactgagga
    18301 ataaccctat attaaccgcg aaagatcccg caatctgaat caaaaaagga taagacggca
    18361 ttagatggcg attcatatcg tccagaccgg ccagccatat ggaaaaaaat tgccccagca
    18421 cgaacacgat gcaaaacgac tgagcccagg caattttttt gtattttaca gcggcaacca
    18481 tgatcaatac cgattgcagg gcaagcagca gaaacagatt tccgtaggcg gccacgataa
    18541 tatcaaaagc tctttgggcc atgtatttga cataaacaac tgtgcaccgg gcatggctga
    18601 tgaaccgtat gatcctttcg ggagaataat taaccctgtc aggaaagacc gaacagaaaa
    18661 tcatatcctc cgtcggcggc aggtctttcg gaaagttccg ataacagcgc ttgtagttat
    18721 cgataaaatg attattgatc gaaaaaggag cggcataata attgatatgt cgcgttctgt
    18781 cgatgacggc catgaattcc tcatctttcc cgtattcgta agccccgctt atacttaaat
    18841 gagcaatcgt attgttcagc gcgatgttac tcaaaacaaa ctggccgttg aactttttat
    18901 tcattccgca atatccgagc acacccgcag ccgtgaagca caatcccagc aatccccaat
    18961 agaggatttt tctttcgtcg cgcagtagaa aaaaacggca caacagaaaa agaagcatga
    19021 ccgctatcag gatcaaatac gtcggcttca gcatgatcag gaaaaacggg aaaatgccga
    19081 tcgccagcgc ggcggaccgt ttcggttttt caagaaaccg gacaagcagg tacagcataa
    19141 agaccgatcc ggcaaagcac aggctctccg gattgagatg gacattctga atcaggatgg
    19201 gatgccagca gccgtaaaag agcgttgcaa tcatgcccac ataaacattg ccgatgacgc
    19261 gtttggaaac gaaatagaac gggatgatag acaagaacga cagggcctgc tgaaaaaaaa
    19321 tgagattgcg gaccagatga tctccggaaa catattggaa gaatttgatc acatacggat
    19381 aaagcggcgt gcggtacagg tcaacgcctc ccctgaacaa atccacggcg tagaaatacg
    19441 aaacgctgtc cgaattatac tgcaccattc cgggattcag gaacccgaag taataaattc
    19501 tatacagaag acaaaccaca agaatcacgc ctaaaaaggc gtaatcaggt atgctggcta
    19561 ttttcccttt caaccggtta ttttctgtca tcacgttgtg ttccacgaag ataatcgaat
    19621 atccattttt ttattaccga ttgcaataaa aaaatctctt aatcatccat tatccatccc
    19681 tcgtcgctgc gctcctggga tagttccgtc agcgctttaa tctgcgcttc gcttgatttc
    19741 agcggacgtc actaccattt cccattgaca tcaatcagca agttccctat gctcttctca
    19801 aaaaaacggg gagctcaaaa tggcacacag actcaacggt aaaaatacga tcatcaccgg
    19861 agcagccaat ggaatcggtc gcgcctccgc agtgcttttc gcccgtgaag gggccaacct
    19921 tgttcttgcc gatatcagcg aaaccgggct tcggcaggtt gaggacgaaa tcaaagggat
    19981 gggaggaaac gtcgttatcc gcaaaaccga cgttgcatcg gaagaagaaa tccaagcgct
    20041 gatcaatctg gccctggaaa aattcaatac gattgacatc ctggtcaaca acgccggcat
    20101 cggcggcggg ctggacgcgc ttgaagatca gaagcccgag gaatggcatc tcgtttatga
    20161 tatcaatgtc atggggccgg tgtatgccac caagcacatc atcgctcata tgaaagaacg
    20221 tcgccgggga tcgatcatca atatcgcatc agtggccggt ctgctctccg gcgccggcgg
    20281 caatgcctac agcgccagca aggccgcttt aatcaacttc acgaaaacgt ccgcctgcga
    20341 agtcggggcc ttcaacgtcc gcatcaacgc cgtctgcccg ggactcattg aaaccggcat
    20401 gacccagccc tttttcgatt gggcccgcag caccggcaag gaaaatcagc tcggcaaata
    20461 ctgcgagttg aaacgggccg cc
//
LOCUS       DAOSME010000043        19311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3459_length_19311_cov_21.180879, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000043 DAOSME010000000
VERSION     DAOSME010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 19311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19311
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3459_length_19311_cov_21.180879"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..709
                     /locus_tag="PLG98_07550"
     CDS             185..709
                     /locus_tag="PLG98_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE33146.1"
                     /translation="METFLEEVNKKINGVPLQRCYHCRKCTAGCPMTKHMEYNPNKII
                     KMIQNGQRDKVLNSSTIWLCLSCETCITRCPNQVDIASMMDVLRQMAIQQGTVREANV
                     LKFHEAFLAGMKFGGRINEPMMMVQYKLKSGDLFSDALLGLDMFKKGKLALLSPRTKD
                     MKSVRNIFEKTKNR"
     gene            737..1585
                     /locus_tag="PLG98_07555"
     CDS             737..1585
                     /locus_tag="PLG98_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOE33147.1"
                     /translation="MKVSYYPGCSLHATGKEYDQSMKAVSRALDIELKEVDDWCCCGA
                     SSAHNTNFDLSIALPARNLIAAEKDAMDVMVPCAACFNRFKTAEHHLKADKDLKAKIE
                     GIVGAKYQGGIAIRNPIDIIFNDIGIDVLAEKVVKPLTGLKPVSYYGCLLLRPPEVCE
                     FENYENPFMLDKMMKAIGADVKNWSYKTDCCGGSLAISKTAIVVEMCDRLMNGAREAD
                     ANCVVTACPLCMANLDMRPSEGKKLPVFYFTELISLAMGLPGSKDTFKSHIFDPRPLL
                     SQLGLI"
     gene            1745..2767
                     /locus_tag="PLG98_07560"
     CDS             1745..2767
                     /locus_tag="PLG98_07560"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE33148.1"
                     /translation="MEKRLIKKDALAGIAKKMTEKFLVWAPVKKEDNVLFEPLQKNAE
                     PLFAYLNSKNAPKNVFFPHTETMMKYTRTPKGMVFSTEENKAVESVLFGVRPCDAHSF
                     VLLDKLFDQEKYKDGYYIAKRQSTTVISLACVKPPYASCFCTSVDGEPVSSDGADILI
                     TDIGSDYLVEFITPKGEKLASYFGDAKADAATETKKNEIAAKAREAITSKIPAHEIKP
                     ILDKNFDNPFWDTIHRKCLACGTCTYMCPTCHCFDISDEVKGSDGVRIRSWDSCMFPL
                     FTKETSGHNPRSSQKERWRQRAMHKFKYYVDMFDCIACVGCGRCVMSCPVNIDIRKIV
                     EDISKL"
     gene            2782..3621
                     /locus_tag="PLG98_07565"
     CDS             2782..3621
                     /locus_tag="PLG98_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOE33149.1"
                     /translation="MQNPYIPYPVVVDKIITEVDTKDIKTFRFKFVNPEDEQKYAYIP
                     GQFGELSIFGKGESPIGIASSPTQKGYVEFTVQKAGVVTSALHEMEEGTVMGIRGPLG
                     NSWPLDFLAGKNIVIVGGGFAFTTLRSLINYMLFEDNRKRFGNITVIYGARNPGLLLY
                     KDELVEWGKRADINLNVTVDKGDATWKGKEGFVPAVCKEVAPSKENAVAVICGPPVMI
                     RFTLPVFFDLGFSKQNILTSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFSLAELDKLT
                     PEY"
     gene            3773..4609
                     /locus_tag="PLG98_07570"
     CDS             3773..4609
                     /locus_tag="PLG98_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOE33150.1"
                     /translation="MNKIIEKENLSESVVRMVLEAPVVAQKRKAGQFIVLKIHEKGER
                     IPLTIVDSDAQKGTITIIFQVVGKTTALLAAMAPGDGIQDVQGPLGNPTEIENFGRVV
                     CIGGGVGVGVVYPLIVALKKAGNEVVSIIGSRTKNLLILEEEIKANSDRLVVTTDDGS
                     YGVHGFVSNVLQQMIDQGERIDRVFAIGPVPMMRVICDVTKPHGIKTIVSLNSIMVDA
                     TGMCGACRVDVGGKTRFTCVDGPEFDGHQVDFKLLADRLRMYCDQEREAYEKHKCGCD
                     GN"
     gene            4575..6014
                     /gene="gltA"
                     /locus_tag="PLG98_07575"
     CDS             4575..6014
                     /gene="gltA"
                     /locus_tag="PLG98_07575"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOE33151.1"
                     /translation="MKNISVGAMETKETKPKKEKVPRQTMPEQDPKERIHNFEEVPFG
                     YSEETAIIEAQRCIQCKKPGCVSGCPVDVKIPEFIKAIAEGRFIDAALKLKETNGLPA
                     VCGRVCPQEDQCEKECILGKKGEPVAIGRLERFAADYERNSGKISIPPAVQSNGKKIA
                     VAGAGPAGLTIAGDLVKLGYDVTIFEALHKAGGVLVYGIPEFRLPKAIVEAEVDYLKK
                     MGVKIETNFVIGRTKTIDELFADGFDAVFIGVGAGAPVFMNIPGENLSGIYSANEYLT
                     RSNLMKAYLFPEYDTPIVRGKKVAVVGGGNVAMDSVRTALRLGAERAMIIYRRTEVEM
                     PARKEEVHHAQEEGVEFLLLHNPVEYIGNEAGWVTGMKCIKMELGEPDASGRRSPVPI
                     QGSEFVIDVDTVVVAVGTMANPIVPATTTGLETNKKGYILTKDESGLTSREGIYAGGD
                     IVTGSATVILAMGAGRKAANAIHEYLSNK"
     gene            complement(6049..6681)
                     /locus_tag="PLG98_07580"
     CDS             complement(6049..6681)
                     /locus_tag="PLG98_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOE33152.1"
                     /translation="MEFSLLQNILLGLSLAAPFGPVNVEIVKRGLNSGFKQAFVTGLG
                     AMTADTTYLTIIFFGLVSFLKMPLLKIFFALAGSLILIYLGVTSIKDFLGKTDYDEKQ
                     NRRFFKNPYVAGYVIALSCPITIVWWTGVFGALLASQDSIQTNFSAYLSCLTILLGCF
                     IWIVFLSAALHWGRKIVNEKFIGFVSLIAGMCLAGFGLAFLCRSVHLIIH"
     gene            6963..8054
                     /locus_tag="PLG98_07585"
     CDS             6963..8054
                     /locus_tag="PLG98_07585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOE33153.1"
                     /translation="MSKYVYNQEAVENLRLSDTSILDRLIDFESARAAHLKKDHAKKD
                     KRMSLTEAISTFIQDGDIITDAGFSYVRTPHQAFHEIIRQGKKNLQMIGAPNTNQSFQ
                     IIYGCVYYSHNSYIGVEMRGIDKSYDRMLKAERVKILSEWSHGGVAQGFKAAQLGMPG
                     VFSKQMLGSDIVRYNPYLKVMQNPMKDKNDPVVFIPALYPDVAIIHCHAADKYGNAYI
                     YGPAVNDLAVAAATRKLIITAEEIVPENTIRYSKEGQIIPFFYADAVVELPFGAVPGN
                     MPGCYYWSRQWWEKLIRLGGVSDENMKAILDEWILGTKDQFDFIDKLGGSRWIVEARR
                     QTKAAEGDNEDIDFSYDEYTLNHDSGLYY"
     gene            8069..8857
                     /locus_tag="PLG98_07590"
     CDS             8069..8857
                     /locus_tag="PLG98_07590"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOE33154.1"
                     /translation="MKDLNAPANPLETLAYIIAQQIHDHSIVYIGTGIPMVAGILAKK
                     THAPNITIVYESGGQDPIEGDMPWSVGCPFTWRKSPTIMEMANSFAQCYNGYVDIGFL
                     GFAQVDMYGNVNTHQIGKDFHKPKVRMTGSGGNNDLSSLTENMVLVGLQTPDKFPDAV
                     DFITSVGHLKGGNSRQEAGLLGSGPVAVISPWGVYDFHPVSKRMRVKSLTPGVTFEIA
                     QSLTGFELLKPDGDIPQTKLITDDALKIIRTEVDPRGVFTTMPT"
     gene            8981..9451
                     /locus_tag="PLG98_07595"
     CDS             8981..9451
                     /locus_tag="PLG98_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE33155.1"
                     /translation="MENIKKILAVSWITQYCQATVRAAIEVASKFDAELSVIHVVDTQ
                     LLQGWNLPFKFHDEENQKELERIKSEINSVVNSEKKEDMKVKTVVKEGNPVEEILKYV
                     EKEKVDLVVLRAHEKSRLEKFMVGSSNDEIILKMPCSILLLKNEPKKKTIRFKA"
     gene            9703..11418
                     /locus_tag="PLG98_07600"
     CDS             9703..11418
                     /locus_tag="PLG98_07600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE33156.1"
                     /translation="MNYLVQPKKYQSLMTDDGSRAIMDKTVAFFEKMGKERLLDDYNK
                     KVWYREFVDFIGREQIFAKLLTPSQYGGGDPDCRWDTARNSEYSELLAFYGLGYWYCF
                     QVSILGLGPIWMSPNEKAKKKAAELLKKGAIFAFGLSERTHGADIYSTETTLTPADNG
                     TWVANGEKYYIGNGNEAEMVSTLGKVKDGTDDYVFFVTNYKHKAYELKKNVISHQEYV
                     SNFALHDYPITEDEILMRGPHAWDSALNTVNIGKFNIGPASIGVVEHCFYEAITHASN
                     RILYGMKVTDMPHVRKNFMDAWLRLVAMKLYQRRSTDYFRNANDKDRRYLLYNPTSKM
                     KVTLQSEDVLNLLWEVIAAKGFEKDTYFHMAAADIRGPSKLEGTVHVNVQLIRKFMKN
                     YFFNPVSYAPVAPDFSAKDDLFLFNQGPTKGLGSVQFHDYKPVFEAGRKLPNVEIFIR
                     QINIFKEMLEKAGPDKAQDLDPSWSLPVGEMFSIVVYGQLILEQAAFDKVDDDILNQI
                     FDFMVRDFARFALQIYGMHNTKEDQRAYCKDIMLIKGQGDDAQYDRVWQKYVAVLNGE
                     YAMSE"
     gene            complement(11491..12288)
                     /locus_tag="PLG98_07605"
     CDS             complement(11491..12288)
                     /locus_tag="PLG98_07605"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOE33157.1"
                     /translation="MFQWFINRRRSKLTAAPFPDAWEDILERNMGHYRLLHDAERAHL
                     RSLIQVFIAEKHWEGAGGLVLNDEIRVTIAAHACLLLLRLSHNYYRNVETVIVYPSTL
                     VAPRRKPGFFEVSLEPLEENYPIVGEAFQQGPVIIVWDAALQGGRQSESGHNVVYHEF
                     AHKLDMLDGAADGTPPLKDRAKYRDWIKTFSHEYLRLRRDTEKGRQTFLDAYGATNEA
                     EFFAVATEHFFEQPLIMEKNAPELYRRLREYYGQDPASRFRQNNCAV"
     gene            complement(12468..13031)
                     /locus_tag="PLG98_07610"
     CDS             complement(12468..13031)
                     /locus_tag="PLG98_07610"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOE33158.1"
                     /translation="MFKKLLLLCSVVLILTSVGYAKEVGGINMPDTLAAGTDTLILNG
                     AGIRTKFFIKAYVTGLYLKKKNSDANAILNADEPMAVRIHIISKLITSDKMKDATMEG
                     FQNSTNGNIAPYKDKIDTFISVFADNIKINDVYDLVYTPAEGTQVYKNKQLKTTTKGL
                     DFKKVLFGIWLCNKPADKALKSQMLGK"
     gene            complement(13272..14495)
                     /locus_tag="PLG98_07615"
     CDS             complement(13272..14495)
                     /locus_tag="PLG98_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOE33159.1"
                     /translation="MPRIFDPNDKYYIDNPGRVKAIQAEMHKRGIDVYLGSRIRTMSF
                     VLDAFCPWRSYLIIPSEGQPAFFTFVIDALRVGDETWLDSDHVRGYGPMGGMDQVSAI
                     TDYIKDELGIKKGRLGYEDGVSTYTAEGNLSHWEYTRLRDALPDFEWVNAHDIIDGLS
                     LIKDKGTIERFRTASLIVDEGHKAARAALENGGYRGMTETVIAGIAALAMRKAGSVSE
                     WNFAGLNEISSGYRTGLGACTPPTTKEIKAGEPLMLDFHAMFKLALGDHSHNYLIDPA
                     TKRQRWHADNFVSIIRKVIDNYRAGTTPGKLAAMMMDFAESRGCADFILPGCEHGIGL
                     FGDEWRIGSIVNPDLPYWTDQDHVYQENEMVVCAMQYAAPEEDIGFRYENDIVITKDG
                     CDVMSKYPLGIEEIS"
     gene            complement(14507..14911)
                     /locus_tag="PLG98_07620"
     CDS             complement(14507..14911)
                     /locus_tag="PLG98_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOE33160.1"
                     /translation="MSELLAYLSPAWCDEAFKRLQTELTPDKMNKVSTSMSNIYKNCP
                     GGKEMFLFIECKEGNITRVETGVGDPPKAEFLIIGDYETFSRISRAELGSQKALMTGK
                     IKLKGNLAKALKLAAVSDRLNKVLAQIPTQYG"
     gene            complement(15201..15671)
                     /locus_tag="PLG98_07625"
     CDS             complement(15201..15671)
                     /locus_tag="PLG98_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15540..15542),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HOE33161.1"
                     /translation="MARVELNVQAPDFTLKDFNGTIVSLSDFQNKKNVMLVFNRGFIU
                     PFCRVHMAQLRQDYEKFVKLDTEILVAGPEDAEAFKKYWVKEKLPFVGLPDPEHHVLK
                     LYGQEVKIFKLGRMPAQIIIDKSGKVRFVHYGHSMMDIPENKELLELLATQIEK"
     gene            complement(15759..16304)
                     /locus_tag="PLG98_07630"
     CDS             complement(15759..16304)
                     /locus_tag="PLG98_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOE33162.1"
                     /translation="MGKGIVDRLKKEYPLEDHWVSFELHPETPPAGVLLAERFKDYDL
                     SSFYEQLCQRGKEVSVVFGNQKILSNSRFALMASEYARDLDRYDSFHENMFHAYFTEG
                     LDIGKTDVIAAVAKKSGLDEKGTRNAIHDDRYASRLNEARKEGKSLGLTGIPLFIINN
                     KYQITGAQPIETFRELLKKIT"
     gene            16620..17018
                     /locus_tag="PLG98_07635"
     CDS             16620..17018
                     /locus_tag="PLG98_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33163.1"
                     /translation="MRDEIIVEYNQEGYVHAKHFGKNNYDLSLEVWRRIKALCKEHNC
                     FKILGENHTTEELSTMDAFNHLNILEEVGITFQYRIAWVDQGTPTAKGLEFVETVVVK
                     NRGLVNGKLFADLEEARRWLLGKDNPQRNS"
     gene            17015..17755
                     /locus_tag="PLG98_07640"
     CDS             17015..17755
                     /locus_tag="PLG98_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33164.1"
                     /translation="MSRILAAPLKSNSHAFMKSSFIPAFIAFGLMSYLAVVQQWSLQE
                     FCWCVWLAGLFYSWTCVMTAAIQMMLTARAQKDYYHANIPLMKKIPPALFALGMIPVA
                     LLIGFVGLYLYTWLFSFYGLFLSVFAEMPPVDLFGRNGFINSDFFTPVTYLTDQYWPM
                     ILATLAANADGLLRATPWKRIIFPFKQNEILRIHVMTLLMPFLAMMTWEFFRDAYQQL
                     TIILLIGIFYLLPKRRFKQENMANNPLK"
     gene            17820..18200
                     /locus_tag="PLG98_07645"
     CDS             17820..18200
                     /locus_tag="PLG98_07645"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOE33165.1"
                     /translation="MGNKAKYKISSSQIDGILEIVLTGEVSKEDVETLQNEIFSIIIN
                     TGAEALLVDVRAIKGRFGYLEAYSRVRHYPTDTPRIHTAIVDNAENADYESFHETTAY
                     NAGLSYKWFTDIEKARSWLKDKIK"
     gene            18207..18764
                     /locus_tag="PLG98_07650"
     CDS             18207..18764
                     /locus_tag="PLG98_07650"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOE33166.1"
                     /translation="MLSNKIIRKTPFGPVCIIWSVSKDHPRIIQIMLSRPGLSAENRV
                     SQLFPESKISSCAEIDHVASSIRAYLEGEDITFHLNLVDLSPFSRFQQSVLLAQHAIP
                     RGKVSTYGLIAVHVGAPGGARAVGNVMAINPYPLIIPCHRTVLSDCRLGGFQSGITMK
                     KALLTQEGVMFDQNGRVVCRQLHYD"
     gene            18957..>19311
                     /locus_tag="PLG98_07655"
     CDS             18957..>19311
                     /locus_tag="PLG98_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOE33167.1"
                     /translation="MPITEILARNAAQYGEEISLVEINPQIKEIKANWKDYELVEPNP
                     EKRYRREMTWREFDDKANRFANFLLDRGLEKGRKVGILLMNCLEWLPIYFGVLKTGAL
                     AVPLNYRYTEDEIKYC"
BASE COUNT         5071 a         4419 c         4902 g         4919 t
ORIGIN      
        1 ctgtccgatg ggcgtggatt taaggaagtt cctcaagaaa ctcgacaagg attgctttga
       61 attcttcggc aaccgcgcag gatccagtat ggacgatatg ccgcctctgg gcaaattcga
      121 tgaacatcac gacaagggag actttgtcta caacccataa ttagcttgag ataatggagt
      181 ttgcatggaa acttttctgg aagaagtaaa caaaaagata aacggggtgc cgctgcaacg
      241 ctgctatcat tgccgcaaat gcacggccgg ctgtcccatg acaaagcaca tggagtacaa
      301 ccccaacaag atcatcaaga tgattcagaa cggccagcga gacaaggtgc tcaacagttc
      361 caccatctgg ctttgcctgt cctgtgaaac ctgtattacc cgctgcccca atcaggtgga
      421 tattgccagt atgatggatg tgctcaggca gatggccata cagcagggca ctgtccgcga
      481 agcgaatgtt ttgaagttcc atgaggcatt cctggccggc atgaaattcg gcggacgcat
      541 caacgaaccg atgatgatgg ttcagtacaa gctcaagtcc ggagatttat tctccgatgc
      601 gctgctgggt ctggatatgt ttaagaaagg aaaactggcc cttttgtcac cccgcacgaa
      661 agacatgaaa tcggttagaa atatttttga aaaaacaaaa aacagataaa gaggtttaag
      721 gttaaggagg ctactcttga aagtttcata ttatccgggc tgctcgctgc acgcgacggg
      781 aaaagagtat gatcagtcga tgaaggccgt cagtcgagcc ctcgacatcg aactcaaaga
      841 agttgatgac tggtgttgct gcggcgcatc gtccgcgcac aatacaaatt ttgatttgtc
      901 cattgctctg ccggcgcgaa acctgattgc cgcagaaaaa gatgcgatgg atgtcatggt
      961 accctgtgcc gcctgtttca accgttttaa aacagccgaa caccatttaa aagccgataa
     1021 ggatctcaag gcgaaaattg aaggtatcgt cggcgcgaag tatcagggcg gcattgccat
     1081 ccgtaatccc atcgacatca ttttcaacga tataggtatc gacgtgcttg ccgagaaggt
     1141 ggttaaacct ctgaccggat tgaaacctgt ttcctattac gggtgcctgc tgcttagacc
     1201 gcccgaggtt tgtgagttcg aaaattacga aaatccgttt atgctggaca aaatgatgaa
     1261 agccatcggc gcggatgtta agaactggtc gtataagacc gattgctgcg gcggttcatt
     1321 ggcgatcagt aaaaccgcga ttgtggtgga aatgtgtgac cggttgatga acggcgcccg
     1381 tgaagcggat gcgaactgcg tcgtcacggc gtgtccgctg tgtatggcca atctggatat
     1441 gcgtcccagc gaaggaaaga aactgcctgt attttatttc acggaattga tatcgttggc
     1501 aatggggctt cccggatcga aagatacgtt caaatcgcat atttttgatc cccggccgct
     1561 gttgtcgcag ttggggctga tttaataaat tgtaagcgaa tgattttaat tataggtgat
     1621 gaaaacggat gaatattgat attatcccgc taagcttcat gcgtttggat aattcgacca
     1681 gcaagcttca gtgcgctaca cttctgaagc ttgggtctca ttaaattctt aattagggaa
     1741 aaatatggaa aaacgtttaa ttaaaaaaga tgcgctggcg ggtattgcca aaaaaatgac
     1801 cgagaagttt cttgtctggg cgccggtgaa aaaagaagac aatgtgcttt ttgaaccttt
     1861 gcagaaaaac gccgaacccc tctttgctta tttgaattcc aagaacgcgc caaaaaatgt
     1921 tttctttccg cacacggaaa ccatgatgaa atacacccgg acacccaagg gaatggtttt
     1981 ctcgacagaa gaaaataagg cggtcgaatc agttttgttc ggcgttcgtc cctgtgacgc
     2041 ccatagtttt gtcctgctgg acaaactttt tgatcaggaa aaatacaagg acggctacta
     2101 catcgccaaa agacagagca caacggttat ttctctggcc tgcgtgaaac caccctacgc
     2161 cagctgtttt tgcacatccg ttgacggcga acccgtttct tcggatggcg cggacattct
     2221 gattacggac atcggctccg attatctggt cgagttcatc acgcccaagg gagaaaaact
     2281 ggcttcctac ttcggcgatg ccaaggcgga cgccgcaacg gaaaccaaga aaaacgaaat
     2341 tgcggcgaaa gccagggaag cgattacgtc caaaatcccc gctcatgaaa tcaagccgat
     2401 tctggataaa aatttcgaca atcccttctg ggatacgatt caccgaaaat gtcttgcctg
     2461 cggaacttgc acctatatgt gccccacctg tcactgcttc gatatcagcg atgaagtcaa
     2521 gggcagcgac ggcgttcgca tccggtcgtg ggattcctgt atgttcccgc tgtttaccaa
     2581 ggaaacatcg ggacacaacc cgcgctcctc gcagaaggaa agatggcgac agcgcgcgat
     2641 gcataagttt aagtactacg tggacatgtt cgattgcatt gcctgtgtcg gctgcggccg
     2701 ttgcgtcatg agctgtccgg tgaatattga tattcgtaaa attgttgaag atatttcaaa
     2761 attataatca gggagttagt aatgcagaac ccgtatatac cgtatcccgt tgtggtggat
     2821 aaaatcatta ccgaagtcga caccaaagac attaaaacat tccgttttaa attcgtgaat
     2881 ccggaagacg aacagaaata tgcttacatt cccgggcaat tcggcgaact ttccattttc
     2941 ggaaaaggcg aatcgccgat tggcattgcc tcgtctccaa cacagaaagg gtacgtggaa
     3001 ttcacggtac agaaagccgg cgtggtcacc tcggcgcttc acgaaatgga agagggaacc
     3061 gtgatgggta ttcgtggtcc tttgggcaat tcctggccgc tggattttct ggcaggcaaa
     3121 aatatcgtta tcgtcggcgg cggtttcgcc ttcaccactc tgcggtcgct catcaattac
     3181 atgctttttg aagacaaccg caaacgcttc ggaaatatca cggtgattta cggcgcacga
     3241 aatcccggac tgctgcttta taaagatgaa ctggtcgaat ggggtaaaag agcggatatc
     3301 aatttgaatg tcacggttga caaaggcgac gccacttgga agggcaaaga aggttttgtc
     3361 cctgcggtat gcaaagaggt cgcgcccagt aaagaaaacg ctgtcgctgt tatctgcggg
     3421 cctccggtca tgatccgttt cacccttccg gtatttttcg atctgggctt ttccaagcaa
     3481 aacattctca cgtccctgga aatgagaatg aaatgcggca tcggcaaatg cggacgctgc
     3541 aacgtgggca gtaaatatgt ttgcaaagac ggtccggtat tttctctggc ggaactggac
     3601 aaactgacac cggaatatta aaaaacgttt cagcaaagaa tctgcgatac gaagaactgt
     3661 tcaatcatgg aatggttggc aggtggtgct gtttttttta ccttaagtat tttttcacgt
     3721 taaaatttaa taaagacgaa cacctgtttt tttcatggag gagaaattaa gcttgaataa
     3781 aattattgaa aaggaaaatt tatccgagag cgtggtcaga atggttctgg aggctccggt
     3841 cgtggcgcaa aagcggaaag ccggacagtt tatcgttctt aagattcatg aaaaagggga
     3901 acgtattcct ctgaccattg tcgattctga tgcgcagaag ggaacgatca ccattatttt
     3961 tcaagttgtg ggcaagacaa cggcgttgct tgcggcaatg gctccgggag atgggattca
     4021 ggatgtgcag ggtcccttgg gaaatccgac ggaaattgaa aattttggcc gtgtcgtttg
     4081 catcggcggc ggtgtcggtg tcggcgtcgt ttatccgctt attgtcgccc tgaaaaaagc
     4141 gggcaatgag gttgtttcca tcatcgggtc acgcacgaag aacctgctga ttctggaaga
     4201 agagatcaaa gcgaattccg atcgtctggt ggtcaccacc gatgacggaa gttacggcgt
     4261 tcatggtttt gtcagcaatg tccttcagca aatgattgat cagggggaac gtatcgatcg
     4321 tgtgtttgcc attggccccg tgcccatgat gcgtgtcatt tgcgacgtga caaagccgca
     4381 cggcattaaa accatcgtca gtctcaattc gatcatggtc gatgctacgg gaatgtgcgg
     4441 agcctgccgt gttgatgtgg gtggaaaaac caggtttacc tgtgtggacg gtcctgaatt
     4501 tgatggtcac caggttgatt ttaaattact ggccgaccgc ctgaggatgt attgtgatca
     4561 ggaacgggaa gcctatgaaa aacataagtg tgggtgcgat ggaaactaaa gaaacgaagc
     4621 caaagaaaga aaaagtgccc cggcagacca tgccggaaca ggaccccaaa gagaggattc
     4681 acaactttga agaagtgcct ttcggctatt ccgaagaaac ggcgattatc gaggcgcagc
     4741 gctgcatcca gtgtaagaaa cccggctgcg tcagcggctg tcccgtggat gtcaagattc
     4801 ccgagtttat caaggccatc gcggaaggtc gtttcattga cgccgccttg aaattaaaag
     4861 aaaccaacgg tctgcccgct gtctgcggcc gcgtctgccc gcaggaagat cagtgcgaaa
     4921 aggaatgtat tctcggcaag aaaggcgagc ccgtggccat cgggcggttg gagcgtttcg
     4981 cggcggacta tgagcgcaac agcggcaaaa tttccattcc tccggccgtg caatcaaacg
     5041 gcaaaaaaat tgccgttgcc ggcgccggtc cagcgggatt aaccattgcg ggcgatctgg
     5101 ttaaactggg ttacgatgtc accatctttg aagctctgca taaggccggc ggcgtgctgg
     5161 tttacggcat tccggagttt cgtttgccga aggccattgt ggaagcggaa gtggactatc
     5221 tcaaaaaaat gggcgttaag atagaaacga attttgtcat cggccgcacg aaaacaatcg
     5281 atgaactttt tgccgatgga tttgatgccg ttttcatcgg ggtgggcgca ggcgctccgg
     5341 tctttatgaa catccccggc gagaatctga gcggcatcta ttcggcgaat gaatatctga
     5401 cgcgctccaa tctgatgaag gcctatttgt ttcccgaata cgacactccg atcgttcgcg
     5461 gtaagaaagt cgcagtggtc ggcggcggca acgtcgcgat ggattccgtg cgtacggctt
     5521 tgagactcgg cgcggaaagg gcgatgatta tttaccgccg caccgaagtg gaaatgcccg
     5581 ccaggaaaga agaagtccat cacgcgcagg aggagggcgt tgaattcctc ctgctgcaca
     5641 atccggtgga atatatcggc aacgaagccg gctgggtcac tgggatgaag tgcatcaaaa
     5701 tggaacttgg cgagccggat gcgtccggaa gacgaagccc cgttcccatt caaggatccg
     5761 aattcgtgat tgatgtggat accgttgtcg ttgccgtcgg caccatggcc aatccgattg
     5821 tccccgcaac caccaccggt ttggaaacga ataagaaagg atatatacta accaaggatg
     5881 aatcggggct gaccagccgc gaaggcattt atgccggagg cgatattgtc accggttccg
     5941 caacggttat tctcgcgatg ggcgcagggc gcaaggcggc caacgcgatt cacgagtatc
     6001 tttccaataa atagttcaat ccggcagccc gggcctgatc ccgggctgtc aatgtatgat
     6061 cagatgcacg gaccggcata gaaaagccaa gccgaatccc gccagacaca tgccggcgat
     6121 caacgaaaca aatccgataa atttttcatt gacgattttt ctcccccagt gcagcgcggc
     6181 ggagagaaaa acgatccaga tgaagcagcc gagcaatata gtgagacacg aaagatatgc
     6241 ggaaaaattt gtttgtatgc tgtcctgaga tgccagcagg gcgccgaaaa cacccgtcca
     6301 ccagacgatg gtgatcggac aagaaagcgc aatcacgtaa ccggcgacgt agggattttt
     6361 aaaaaaccgc cgattctgtt tttcatcata gtccgttttg cccaggaaat cttttatgct
     6421 ggttacaccg agataaatca aaatcagact gccggcgagg gcaaaaaata ttttcaacaa
     6481 aggcatcttc aaaaacgaaa cgagaccgaa aaagatgatt gtcagatacg ttgtgtcggc
     6541 cgtcatggcg cccaatccgg taacaaaagc ctgtttgaat cccgagttca aaccgcgttt
     6601 gacgatttcc acattcaccg ggccgaacgg agcggccagc gaaagaccaa gaagaatatt
     6661 ttgcagtaaa gaaaattcca tactgttttt gataccacag aagcagcggt cttgtcactt
     6721 tctcgttatg taaatttcca ttctccattt attggcatat ttcacatttt taggttgata
     6781 aaatattatt tcatattttg ctgcaaaaac atcagtttta aggggaattt ttcttcattt
     6841 ggtctccgtt aaaaaataac aaatatttta gttatatcaa attgttacga taatacttga
     6901 ccctctctga aggtcagggt aagatgattc aactgttatt gcatgaatag gagggcaaac
     6961 agatgtctaa atatgtgtac aaccaggaag cagtcgaaaa tctcagatta agtgacacat
     7021 ccatcctgga ccggttgatc gattttgaat cggcccgggc cgcacacttg aaaaaggatc
     7081 acgcgaaaaa ggacaaacgt atgtcgctaa ccgaagcgat ttcaacgttt atacaagatg
     7141 gagatatcat aacggatgca ggattttcct atgtccggac gcctcatcag gcgttccatg
     7201 aaattattcg tcaaggcaaa aaaaatcttc agatgatcgg agcgcccaac accaatcaaa
     7261 gttttcagat tatctatggc tgtgtttatt attcgcacaa ttcatacatc ggggtggaga
     7321 tgcgcggaat tgataaaagc tatgaccgga tgctcaaggc ggaacgggtc aaaattcttt
     7381 ctgaatggag tcacggcggt gtggcacagg gattcaaggc tgcccaactg gggatgccgg
     7441 gtgttttcag caagcagatg ctgggcagtg atattgtccg gtataatcct tatcttaagg
     7501 tcatgcagaa tccgatgaaa gacaaaaacg atccggtggt attcattccc gccctgtatc
     7561 cggatgtggc cattattcat tgccatgcgg cggataaata cggcaacgcc tatatctacg
     7621 gtcccgcggt caatgacctg gccgtagccg ctgcgacgcg caagctcatc attaccgctg
     7681 aagaaatcgt tcctgagaac accatccggt acagcaagga ggggcagatc attccgttct
     7741 tttatgccga tgcggttgtg gaactgcctt tcggagcggt tcccggcaac atgcccggct
     7801 gttactactg gtcgaggcag tggtgggaaa aattgatccg tttgggcggc gtgtccgatg
     7861 agaatatgaa ggccattctc gatgaatgga ttctgggcac caaagatcag tttgacttta
     7921 ttgataaact gggcggttcc aggtggatcg tcgaagcccg ccgtcagacc aaagccgccg
     7981 aaggcgacaa tgaagacatt gacttctcat atgacgaata taccctgaac catgattcgg
     8041 gcctctacta ttaagaaagg agaatcccat gaaagattta aatgcaccgg ccaatcctct
     8101 tgagacactg gcttatatca ttgcccagca gattcacgat cattcgatcg tttatatcgg
     8161 caccggaatt cccatggtgg cgggaattct ggccaaaaaa acacatgccc ccaacatcac
     8221 gattgtgtac gaatccggag gacaggaccc cattgagggg gatatgccct ggtcggtcgg
     8281 ctgtcccttc acgtggcgga aatcaccgac gattatggaa atggccaact cttttgcgca
     8341 gtgttataat ggttatgtcg atatcggctt tctggggttt gcccaggtgg acatgtacgg
     8401 caacgtgaat acccatcaga tcggtaagga ttttcacaag cccaaggttc gcatgacagg
     8461 aagcggagga aacaacgatt tgtcatcgct tacggaaaat atggttctgg tggggctcca
     8521 gacgccggat aagtttccgg acgcggttga tttcatcacg tccgtgggac atctgaaggg
     8581 cggaaattca cggcaggaag ccggtcttct cggtagcggg ccggttgcgg ttatcagtcc
     8641 ctggggtgtt tatgattttc atccggtcag caagcgcatg cgggtcaaat cgctcacgcc
     8701 cggcgtaacg tttgaaatcg cgcaatcgct cacgggattc gaactgctga aacccgatgg
     8761 agacattccg cagacgaagt tgattacgga cgacgcactg aaaattatac ggacggaggt
     8821 ggatccgcgc ggcgttttta ccaccatgcc gacatagcgg aaacacgttt ttattccgtt
     8881 tgaaatatac ggacagatta ccggaaaacg aagattgttt ttggtggaac ggcatgtttg
     8941 cacaatccat atcattaacc attcattcag gagggcgatt atggaaaaca tcaaaaaaat
     9001 tcttgctgta agttggatta cccagtactg tcaggcaacg gttcgagcgg ccattgaagt
     9061 ggccagtaaa tttgatgccg aactgtccgt gattcatgtc gtcgatacgc aattgctgca
     9121 aggatggaat ctgcccttca aatttcatga tgaagaaaac cagaaagaat tggagcgtat
     9181 taaatcggaa ataaactccg tggtgaacag cgaaaagaaa gaggatatga aagtcaaaac
     9241 ggttgtcaag gaaggcaacc ccgttgaaga gattctaaaa tatgtcgaaa aggaaaaggt
     9301 agacttggtt gttctgaggg cgcatgaaaa aagccggctg gagaaattca tggtcggcag
     9361 cagtaatgat gaaattatcc tgaagatgcc ctgttctatt ttattgttga agaacgaacc
     9421 gaaaaagaaa accatccggt ttaaagcctg aggatggaga gcaattttgc cttggaaagc
     9481 tcttctgcct gtgttaaaca ggcaggagat agccgtcggt tagaaaaaca ggatgcggac
     9541 cgccatgtcc acaatgcaat attcatgttt ctatcaccgg tctattttca gaatggtttc
     9601 cccatacacg ggggataacc acccgcaaag ggatttcggg acgctgtctt aaagacaaga
     9661 taatcaaata ttataattgt ttccagtaaa aaggaggatc agatgaatta tttagtgcag
     9721 ccgaaaaaat atcagagcct tatgaccgat gacggttcca gagccatcat ggataagacc
     9781 gtagctttct tcgagaaaat gggcaaagag cgtctgctcg atgattacaa caagaaagtc
     9841 tggtatcggg agtttgtcga ttttatcggg agggagcaga tttttgccaa attgctcaca
     9901 cccagccagt acggcggagg cgatccggat tgccgctggg ataccgcccg caacagcgaa
     9961 tatagcgaac tgctggcttt ctatggcctg ggctactggt attgttttca ggtcagcatc
    10021 ctgggactcg gtcccatttg gatgagtccc aatgaaaaag ccaagaagaa ggctgccgaa
    10081 ctgcttaaga aaggggcgat atttgccttc ggtttgtccg agcggacgca cggcgcggat
    10141 atctattcaa ccgaaacaac gctgacgccg gccgataacg gtacctgggt tgccaacggt
    10201 gagaaatatt acatcggcaa cggcaatgaa gcggaaatgg tttccaccct gggcaaggtc
    10261 aaggacggta ccgacgacta tgttttcttc gtcaccaatt acaagcacaa agcctatgaa
    10321 ttgaagaaga acgttatttc tcatcaggaa tacgtttcca atttcgcgct tcatgattac
    10381 ccgattaccg aagacgagat cctaatgcgc gggcctcacg cctgggattc ggcgctcaat
    10441 accgtgaaca tcggcaaatt caacatcggt cccgcgtcca tcggcgtggt cgagcattgc
    10501 ttttatgagg ccattaccca cgcgtccaat cgcatcctct atggcatgaa ggtgacggat
    10561 atgccgcatg tgcgtaagaa ttttatggat gcctggctca ggctggtggc gatgaagctg
    10621 tatcagaggc gttccacaga ctacttccgc aacgccaatg acaaagaccg gcgctatctt
    10681 ctttacaatc ccaccagcaa aatgaaggtt accctgcagt cggaagatgt tttgaatctt
    10741 ttgtgggagg ttattgccgc caagggcttt gaaaaagaca cctactttca tatggccgcc
    10801 gccgatatcc gggggccgtc caaactggaa ggaacggtgc acgtgaacgt tcaactcatc
    10861 cgcaaattca tgaaaaatta tttcttcaac cccgtgtcct atgctccggt cgctcccgat
    10921 ttctctgcta aagatgattt gtttctgttc aatcagggac cgaccaaagg cctgggatct
    10981 gtgcagtttc acgattacaa gccggttttt gaagcgggaa gaaagctgcc caatgtggag
    11041 attttcatcc ggcaaatcaa tatctttaag gaaatgcttg aaaaagcggg tcctgacaag
    11101 gcgcaggatt tggatccctc ctggtcactg ccggtgggcg aaatgttctc catcgtggtg
    11161 tacggacagc tcattttaga gcaggctgcc ttcgacaagg tggatgatga tatcctcaat
    11221 cagattttcg atttcatggt gcgcgacttt gcccgctttg ccctgcagat ctacggtatg
    11281 cacaatacca aagaagacca gcgcgcctac tgcaaagaca ttatgctgat taaaggccag
    11341 ggcgacgatg cccagtatga cagggtctgg cagaaatacg ttgccgttct gaacggcgaa
    11401 tacgcgatga gtgaataggc tgcacaaacg gccgaagacg ttcggacatt ggattgaagg
    11461 gcgaagcaca atggcttcgc cctttttttt tcaaacggcg cagttgtttt gacggaaacg
    11521 cgaggcggga tcctgcccgt aatattccct gagtctccgg tagagttccg gggcgttttt
    11581 ttccataatg agcggttgtt caaagaaatg ttccgtggcc acggcgaaga attccgcttc
    11641 attggtggcg ccgtaggcgt cgagaaacgt ctgtctgcct ttctcggtgt cgcgtctgag
    11701 acgaagatat tcgtgtgaaa atgttttgat ccagtcgcgg tatttggcgc ggtctttcag
    11761 cggcggggtc ccgtcggctg cgccgtcaag catatccagc ttatgggcga actcgtgata
    11821 aacgacatta tgtcccgatt cggactgacg gccaccctgc aacgcggcgt cccagacaat
    11881 gatgaccggc ccctgttgaa aggcttctcc gacaatcgga taattttctt ccagcggctc
    11941 cagcgagact tcgaaaaaac cgggtttgcg ccgaggcgct accaacgtgg aggggtaaac
    12001 aatgacggtt tcaacattgc ggtaatagtt atgggacaat ctcagcagga gcaggcaggc
    12061 gtgagccgca atggtcacgc ggatttcatc gttcagaaca agccctcccg cgccctccca
    12121 gtgtttttca gcaatgaaca cctgtatcag cgaacgcaga tgagcgcgtt ctgcatcatg
    12181 gagcagacgg tagtgtccca tattgcgctc gagaatgtcc tcccaggcat cgggaaatgg
    12241 cgctgccgta agcttgctgc gtcggcgatt gatgaaccat tgaaacaaat ggggttcctt
    12301 ttacttcaag tcaataaaaa atattttggc aaatgaatag atccctctgg tgtttctgaa
    12361 atggaaggtt cacactgatt caaagaaaaa tcaagggcga aaagccttct gttcgatgga
    12421 agtcttttcg ccctgagaag gcaatctttt ttaaaccatg tcgatcttta tttgcccagc
    12481 atctgacttt tcaatgcctt gtcagccggt ttgttgcaca gccatatgcc gaaaagcact
    12541 tttttgaaat ccagcccttt ggtggtggtc ttcaactgtt tgtttttgta tacctgcgtt
    12601 ccttctgcag gggtgtatac gaggtcgtaa acgtcattga tcttgatgtt gtccgcaaac
    12661 acagagatga acgtatcgat tttgtcttta tagggcgcga tattgccgtt ggtggaattc
    12721 tggaatcctt ccatggtggc gtctttcatc ttatcgctgg tgatcagttt ggaaatgatg
    12781 tggattctga cggccatggg ttcatcggcg ttcagtatcg cgttggcatc gctgtttttt
    12841 ttcttgaggt acagcccggt gacatacgct ttgatgaaga atttcgttcg tatgccggcg
    12901 ccgttcagga tcagcgtgtc agtgccggca gcgagagtgt ccggcatgtt gatcccgccg
    12961 acttcttttg catagcccac ggatgtaagg ataagaacga cagagcacaa cagcaataat
    13021 ttcttaaaca taatgaacac ctctcttttt ttattttagc ccgcttaagg ccaagtgaat
    13081 gctgaaggat aaaatattcc ggttccgctt cgggaccttt ctttctgtgc ggggagtata
    13141 gagacaaagc cttctgtctg tcaattattt atttccatat ctttttaaag agattgcccc
    13201 gggactgcgg cctgtcgggc aatggacagg ccttccggcg ccgtcagaag cgccggaagg
    13261 cgagaggacg tttaagaaat ttcttcaatg ccgagcggat atttcgacat gacgtcacag
    13321 ccgtctttgg tgatgacaat atcattttca taacggaagc cgatatcttc ttccggtgcg
    13381 gcgtactgca tcgcgcaaac gaccatttca ttttcctgat aaacatgatc ctgatccgtc
    13441 cagtagggca ggtcgggatt gacaatagag ccgattcgcc attcgtcgcc gaacagaccg
    13501 atgccgtgtt cgcagccggg cagaataaaa tcagcgcagc cgcgggattc cgcgaagtcc
    13561 atcatcatgg cggccagttt tccgggcgtg gttcccgcgc ggtaattatc gatgactttc
    13621 cggatgatcg agacaaaatt atccgcgtgc cagcgctgac gttttgttgc gggatcgatg
    13681 agataattgt gcgagtgatc gccaagcgcc agcttgaaca tggcgtgaaa gtccagcatc
    13741 agcggttcgc ccgctttgat ttctttcgtt gtcggcggtg tgcatgcgcc caggccggtg
    13801 cggtaaccgc tggagatttc gttgagcccc gcaaaattcc attccgatac gctgcccgct
    13861 ttgcgcatgg ccagcgcggc aataccggcg ataacagttt ccgtcatgcc cctgtagccg
    13921 ccgttttcga gcgcggcccg cgccgctttg tggccttcat ccacaatcag tgacgccgtg
    13981 cggaagcgtt caatcgttcc cttgtctttt atcagggaaa gcccgtcgat gatatcgtgc
    14041 gcgtttaccc attcaaaatc cggaagagca tccctgagac gggtatattc ccagtgcgac
    14101 aaatttcctt cggcagtata ggtggaaacg ccgtcctcat atcccagccg tccttttttg
    14161 atgccgagtt cgtccttaat gtaatcggtg atggcggaca cctgatccat gccgcccatc
    14221 gggccgtagc cgcggacatg atcgctgtcc agccaggttt catcgcccac gcgcagcgcg
    14281 tcgataacaa atgtaaaaaa agccggttgt ccctcggacg ggataatcaa atagctgcgc
    14341 cagggacaaa aggcatccaa aacgaaactc atcgtgcgga tgcgtgaacc gaggtaaacg
    14401 tcgatgccgc gtttgtgcat ttcagcctga atggctttga cgcgtccggg gttgtcgata
    14461 taatatttat cgttgggatc gaatattctg ggcatgattt ctctccttat ccatattggg
    14521 ttggaatttg ggccaggact ttattcagcc tgtccgatac agctgcgagc ttgagcgcct
    14581 tcgccagatt ccccttgagt ttgatttttc cggtcatcag cgccttctga gagccgagtt
    14641 ccgcgcggga aattctggaa aacgtttcat aatcgccgat gatgagaaat tcagccttgg
    14701 gcgggtcgcc gactccggtc tccacacggg ttatgtttcc ctccttgcat tcgatgaata
    14761 aaaacatttc tttcccgccg gggcagttct tgtaaatgtt ggacatcgat gtggaaacct
    14821 tgttcatttt atccggtgta agttccgtct gaagtcgctt aaatgcttcg tcgcaccatg
    14881 ccgggcttaa gtaggcaagt aattctgaca tgctgatacc tcctgcgttg tctgattccg
    14941 gacgtcatga acatgacatc cggatgggta cccctttaca ggtgtttttc gcttctcgcc
    15001 aaaaagttga cggagcactt tctcatcaat ataaaattat aggggcaaag aagaggtaag
    15061 tcaatgtcaa taaacctcaa ggataccaaa agcttgtcat acaaacacag cctcaggcgt
    15121 ccgccataac catgcttgct gatggtgacc tgaccgcagc cttcaggttg ttggagttgt
    15181 gaaaacgcaa acccgttttt ttatttttct atttgcgttg ccagcaattc gagaagctct
    15241 ttgttttccg gaatgtccat catcgaatgg ccgtaatgga caaaacggac tttgccggat
    15301 ttgtcgatga tgatctgcgc gggcattctg cccagcttga atatcttgac ctcctggccg
    15361 tacaatttca acacatgatg ttcgggatct ggcaggccta caaagggcag cttttctttc
    15421 acccagtatt ttttgaaagc ttcggcatct tccggtcctg caaccagaat ttccgtatcc
    15481 agtttgacaa acttttcgta atcctggcgc aactgcgcca tgtgcacgcg gcagaacggt
    15541 cagataaaac cgcggttgaa aaccagcatc acattctttt tgttttgaaa atcggaaagc
    15601 gaaacgatgg tgccattgaa atccttcaac gtaaaatccg gcgcttgaac atttaattcc
    15661 actcgggcca taagttaacc tccttaacta tttttaaatg atacgctgaa attttcaatt
    15721 ttcaattcgt ttttaacgta aggataggcg tatatatctt aagtaatttt tttcaatagc
    15781 tcacggaagg tttcaatcgg ctgagcaccg gtgatctggt atttgttatt gatgatgaag
    15841 aggggaatgc ctgtgaggcc aagcgatttt ccttcctttc tggcttcgtt caggcgtgac
    15901 gcataacggt catcgtggat ggcgttgcgt gttccctttt cgtcaagccc gctttttttc
    15961 gcaacagcgg caataacatc cgttttgcca atatcgagac cttcagtaaa ataagcgtga
    16021 aacatatttt catgaaacga atcgtatcgg tccagatcgc gggcatattc ggaagccatt
    16081 agtgcgaatc ttgaattaga aaggattttt tgattgccaa acacaacact tacctctttg
    16141 ccgcgttggc acagttgctc ataaaatgaa gaaagatcat aatccttgaa tctttcggca
    16201 agcagcactc ctgccggcgg cgtttccgga tgaagctcga agctcaccca gtgatcctcc
    16261 aagggatatt cttttttcag ccggtcgaca atgcccttgc cgatgtagca gaagggtcag
    16321 atatagtcgg aatatatttt gaggtggatt gtcattttat gcctcgaaat tttcgttaaa
    16381 tatactcgca tttatcgtaa ttttacatcg ttttttaaaa aagaaatatt gcaatatccg
    16441 atgaaaaaac gtaagctcaa ctttcaaaaa aaatgttttg ccttcgataa aatttgcgct
    16501 gtaaatgccc tgtggttttt ggattggttt gatcggtacc gtggaagcct gtgttccatg
    16561 tgcaacacag agaaatgaat tcagtcacat tcattctgaa tgttgaggga ggatgtacga
    16621 tgcgcgacga gatcatcgtg gaatataatc aggaaggcta cgtccatgca aagcatttcg
    16681 gtaaaaacaa ttatgacctc tcgcttgagg tatggaggcg tatcaaggcg ctatgcaagg
    16741 aacataactg cttcaagatt ctgggagaga atcataccac ggaagaactc tccacaatgg
    16801 atgctttcaa tcacctcaat atcctggaag aagtgggaat aacctttcag taccgcatag
    16861 cgtgggtgga tcaggggacg ccgactgcca agggactcga attcgtggaa acggttgtcg
    16921 tgaaaaaccg cggtctggta aacgggaaat tattcgctga ccttgaagag gccaggcgct
    16981 ggctgttggg caaagacaat ccgcagcgca attcgtgagc cgcattttgg cggcgccgct
    17041 gaaatcaaac tcgcatgcct tcatgaaatc atccttcatt ccagccttca ttgccttcgg
    17101 cttgatgtcc tatctggcgg ttgtccagca atggtcattg caggaattct gctggtgcgt
    17161 gtggctggcc ggactttttt attcgtggac atgcgtgatg acggctgcga ttcagatgat
    17221 gctgactgca cgggcacaaa aagattatta ccacgcgaat attcccctga tgaagaaaat
    17281 cccgccggcg ttgtttgccc tgggaatgat tcccgtggca ttgttgatcg gatttgtcgg
    17341 cctttatctt tatacctggc tgttttcttt ttacggatta tttctttccg tctttgccga
    17401 aatgccgcca gtcgatcttt ttggcaggaa cggttttatc aattcggatt ttttcacgcc
    17461 ggtcacttac ctgacagatc agtactggcc gatgattcta gcgacgcttg ccgcaaatgc
    17521 agatggcctg ctgcgcgcaa ccccctggaa acggatcata ttccccttca agcaaaatga
    17581 aatcctgcgc atccatgtga tgactctgct gatgcctttt ctggcgatga tgacctggga
    17641 atttttcagg gacgcttatc aacagttgac aattattttg ctcataggta ttttctacct
    17701 gcttccgaaa aggaggttta agcaagaaaa tatggcaaat aatccactga aataattgaa
    17761 acgccagtga cgtctgcaag tcgattccgg atattaaatt taatttaagg agttctgtaa
    17821 tgggaaataa agcgaaatat aaaatttcat catcgcaaat agacggaata ctggaaatag
    17881 ttctgacagg cgaagtgagc aaggaggatg ttgaaacgtt acaaaatgaa atcttctcca
    17941 tcatcataaa cacaggcgct gaagctttgc tggtggatgt tagagcgata aagggacgat
    18001 ttggctatct ggaggcttat tccagggtca gacactatcc aaccgatacg ccaagaatac
    18061 atacggcgat tgtagacaat gccgaaaatg ctgattacga aagttttcat gaaaccacgg
    18121 catacaatgc cggtctttct tataaatggt ttaccgatat tgaaaaagcc aggtcctggc
    18181 tcaaagacaa aataaaataa actattatgt taagtaacaa aattatcaga aaaactcctt
    18241 tcgggcctgt ctgcattatc tggtcggtgt ccaaagatca tccgcgcatt attcaaatta
    18301 tgctttcacg tccgggcctt tcggctgaaa atcgcgtatc gcaacttttc cctgaatcaa
    18361 aaatatcgtc ctgcgccgaa atcgatcacg tcgcttcttc catcagagcc tatctggagg
    18421 gggaggatat aacgtttcat ctgaatcttg ttgatttgtc gcctttctca agattccagc
    18481 agtcggtttt gctggcgcag catgcgattc cgcgaggcaa ggtcagcacg tacggactca
    18541 ttgccgtcca tgtgggagcg ccggggggag cgcgggcggt gggcaatgtg atggcaatca
    18601 atccatatcc tttaatcatt ccttgtcacc gaaccgtcct ttccgattgt cgattgggtg
    18661 gttttcaaag cggtattacg atgaaaaagg ctttattgac acaggaaggc gtgatgtttg
    18721 atcagaatgg aagagtggtt tgtcgtcaat tgcattacga ttaaagaggg gcagccatca
    18781 gaaatagcgc ttttgtttca tttattttgt ccaaaacatt cttgacatac attcctgaaa
    18841 ttcgtatcgt attttacgtt taacaggttc ctgataagcg tgttgatcat gaactgacag
    18901 gataaaagtc gcctataaag aacctgcaaa acagtaaaga ttagaaggaa tcagaaatgc
    18961 ccattacaga aattcttgcc cgcaacgccg ctcagtacgg cgaagagata agtcttgttg
    19021 aaattaatcc gcaaatcaaa gaaatcaagg caaactggaa ggactatgag cttgtcgagc
    19081 ccaatcctga aaaacgatac agacgggaaa tgacctggcg cgaattcgac gataaggcca
    19141 accgctttgc caattttctg ctggacagag ggttggagaa gggccggaag gtcgggattc
    19201 tgctgatgaa ctgtctggag tggctgccga tttatttcgg tgttttgaaa accggagcgc
    19261 tggccgtgcc gctcaactac cgttataccg aggatgaaat caaatactgc c
//
LOCUS       DAOSME010000044        18351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3662_length_18351_cov_24.294764, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000044 DAOSME010000000
VERSION     DAOSME010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 18351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18351
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_18351_cov_24.294764"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..352)
                     /locus_tag="PLG98_07660"
     CDS             complement(53..352)
                     /locus_tag="PLG98_07660"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HOE33168.1"
                     /translation="MIHIIPVNKLSARALRGVIEEFISRAGTDYGAVDASMETKFKQA
                     KASLLNGSVVLVFDDETETTNMFRADDPILKKIDAIPEDTETQEAILAEMIKPEK"
     gene            767..4156
                     /gene="recC"
                     /locus_tag="PLG98_07665"
     CDS             767..4156
                     /gene="recC"
                     /locus_tag="PLG98_07665"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOE33169.1"
                     /translation="MGLHVVFSNRLEILKDILLEDLSRIPADPFEAQHVVVPGTAISR
                     YLQLAIADEKGICANVAFDYPGSWLWKLARLVDDKVPERSAVDPEIMTWMILRILGDN
                     RLAAYPRLAGFIQKADDLMLFELARSVAHVFDHYATYRPDWLVAWSEGKRIKDFREEC
                     ADEAKVYEEWQMEIWRTVTQELKLGSTHPLKIFLDKIMTGKTPGAASDLPGRAAIFAL
                     PVIPPIYLQTIRRLSEVMDITFYLLNPCREYWFDIVPPKQLAYLKKIGRDAHTEVGNP
                     LLADWGRSTQSAIDLIYDDASSANTLETSRFIEPEGQALLAQLQRSIFNMQELDPGSV
                     QLPHNDRSVEIHCCHGTVRELEVLHDRLLSLFCADQTLSVDDVVVLTPDIDELGPAVD
                     AVFGTVPKDRFIPYIISGRAMAGTNPYLNMLMGLLDLTASRMPASLVFDLLSQPLVAS
                     RFGVDNDRLNSIRSWLNGSGIFWGIDGEHRRELGLADEDRHTFRRGLDSMLLGVAIPC
                     LDEPIAGYFPYEDLEGSRAETLGQLWLFVERLAFWKNRLKNPLPANDWQDLLNRMLAD
                     FTECGRDAQGAYDQLVGAIAELADNWRAANLTKAISSRVIKAALADADVRRRGAVPSG
                     TVTFASLSAMRGIRYRVVCLVGMNDGSFPGRAYSPEFDLIPKAEPRRGDRQRNLEERG
                     IFLDALLAAGEILHISYTGRDQRDNAIMPPSVLVAKLLDYLSLAMAPENATANDLATA
                     RRQLTVDHPLQPFSRRYFDHSDPRLFSYVGQYATALNRGSKSTPVKKKADLRSDEQEE
                     DMPDPKTPFFNGYGAPPKSPDTESTTLTVDDLSYFLMNPSRFFLRRQLRIQLPQEEDV
                     IADEEPLLFEFSEERDAAAMIIEACRKQKRVITIQDALAILQASPSSPPGSACEAALS
                     GIWPKVSLLAERLLSATTGEKLQPVYSLLSIDVEGETWQLNADLMELRLCGLVFYRCD
                     DLRDFDHLNAWVRHLVLCVCRPEGVLPQTRHVALDGDLIFGDIAWEQAAKHLGNLLAL
                     YRDGLFEPVPFFRKSAWKFVQKDMNAAKSKWSGGFNAGGKAESADPWHALAWRGLEAD
                     DVLNERFKEIAHCVFAPILANEKLEKIVTEEEKGEKRGRKK"
     gene            4140..7445
                     /locus_tag="PLG98_07670"
     CDS             4140..7445
                     /locus_tag="PLG98_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE33170.1"
                     /translation="MAGKNKKVEPLDVFTCDLNGIANIEASAGTGKTWAICGLYVRLI
                     AEMDLPVDQILVVTFTNAATAELRSRIRDRMVEVQHCLDTGRCLSPDPFAAGFLSRMD
                     RLGIRAKAGENLKNALLSFEESAIFTIHSFCQRTLGELPFSAVQPFNVEVEADDSEII
                     RETAADYWRCMTQHYTADEINALLQNKINPSWIAGEIRRLLNRPLAKLVMPETNPSEN
                     PASRRAVWIKRFILKTAPRAIRKTKQDRHVISYNDMLYNLHDVLTLGQFPWLAGVLRK
                     RFPVALIDEFQDTDPLQLEIFRAIYFDKGTLFMVGDPKQAIYSFRNADLNTYLAARRQ
                     AQRQWTMIHNQRSEEKLIFAINAVFGANKNVFGDGEKIPYRDVRRGTKPLKELQGDPE
                     PREPFSVWLFKTGSKAEPLKAGDARSLALAATAKEMARLIDGGMRGTIQIGDENLQPR
                     DIAVLVRTKAQGRQVKEALADYGIGSVDLTERSVFATVEAEEMKTVLRAIAEPSQAGL
                     LRAALSTMLLGFGSRELADLNADETEFNRIVGQVNEYRQAWLGSGFGAMWRRLIAQER
                     VIERLLPLKNGERKITNLMHLMELATGAEETHRGIEALLRWLSEKRSAGDEEDVAQLR
                     LESDENLVQVVTKHKAKGLEWPIVFCPFVWSDRSRSLKDEVCTYHEGDTLVLDYATDD
                     EKKKKQEDEAGEERVRLIYVALTRAANRCYIVAGPFWQPSGKGATEKKAKKSILNWIV
                     AGDEIKHGVWAEKKEVTVDSMHQAWQRLLTSNKGIGGIPWPDKKETVRPVICKEKRKY
                     EARKVGRLPGVAWKISSYSSLTASSLEGGRQHEPATQDYDVIPLAIEDHDPPQVLRAK
                     LSGDIIDFPAGTTTGHCLHAVFERIDFQDESGWKDIITNVLANYPPVDALEKRDDQVS
                     RIHGMLKDVMSTPLPTGFKLGDVARAKRFSEFEFAYSVRHLDHAKLKNLLDKHGCRIP
                     PLDFPDLEGYLRGFIDLVFEHAGKWYVLDWKSNKLGISAAEYSRSSVDEAMFNHAYEF
                     QGLIYLIALHRYLKLRISKYDYSRHMGGILYLFVRGVRPAWPSAGIWHKLPEPALIES
                     LDALFSSENRRTV"
     gene            7451..9268
                     /gene="recD"
                     /locus_tag="PLG98_07675"
     CDS             7451..9268
                     /gene="recD"
                     /locus_tag="PLG98_07675"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOE33171.1"
                     /translation="MRKHENIPAADYASPLSRGLADFLKRLSLTLGATEEAARTAGDA
                     ARACAQALAEGDVCANFSLLAAAEGKPAQDVRKLLDASLVASSAGADKKLPLVYDDEG
                     QIYLYRYFAYERKLADAIIRRLPGPAARTVSAESRQFLEKRFAENARELSGRPDWQKI
                     AVEMAFTNRLTIISGGPGTGKTTIVTALIAALAINDHPPRIALVAPTGKAAARMEESM
                     RSQMSTLDAELRKRMPERASTIHMLLGASPAGNTFRFNENNPLPYDLIVVDEASMIDL
                     SLASRLFSALHPDTGIVLLGDKDQLAAVEAGAVFAELAQQTSVPDNMDKLSTGSGTPP
                     LNAQPNEKGPLSSCVVWLKENYRFSAASPIAKVAGLVVNGEGEQLIEWLQNEKGDNIK
                     WEAFDEGLPSRVVGALVKGFEPFVEAVNQGDPEKVLAAYEKFCVLCVIRNGRRGVNGI
                     NDVIARQLRLKISGEGPGEGPWYDGRPIMITENDYGLGVFNGDIGVALKTKDGHRRVW
                     FMDKDGGLRFVPPSSLPAHQTAFAMTVHKAQGSEFENVSLILPEQDTPVLTRELIYTA
                     VTRAKKTLLIYGSADILKLAVGRPTSRRSGLAKKISTPP"
     gene            complement(9415..12081)
                     /locus_tag="PLG98_07680"
     CDS             complement(9415..12081)
                     /locus_tag="PLG98_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE33172.1"
                     /translation="MINIFPENDYNQTLRIKRFLIAFGAYVMWNAITIASYLLGLTKV
                     PLSILVAGVTANIVVNILLYAVFRTGFNKKAKDPSLTLLQMVIATFWIMVVMYYAYEA
                     RGACLLVYMVVLVFGFFRLRVRQFLLLSLYALVNYSAVIFLVYKLHPELINPKLDLLN
                     IIVLAIILPWFAMIGGYINGLRASITNALTTIERLTDNIKDVIFVVDMNLIYTYVSPS
                     VKTLRGYEPEDVMKQNLADSFAPTSLDTAMETLSEMMEMGKSKSQDSPERTLLLEINR
                     KDGNPVWTETKFSLVRDKEQYITGVLGVSRDISERKRAEEQYRLLADHMTDQVWLFDL
                     NLKFQYFSPSSEKASGYTFTEIKEISLDKLLTEESFQKALEMFSEEMAKAEIVPPPPD
                     YKRSLEVEFRCKDGRLISLDTTLSFIQDKNGKPLSILGESRDVTERKRAEAQIQYLAT
                     HDNLTQLPNRMMFNHLLNQAIKSAKRNKRQLALLFIDLDRFKIINDTMGHDAGDRLLK
                     EMAKRFRQTLRAADVIGRPKDKEDVVGRLGGDEFIILIEDVTDLANVAIVAQKILGTV
                     MKPMVLLGEECRVTSSIGISVYPTDGEDEQTLMKKADMAMYFAKEEGKNNYQFYSQNI
                     QSQSYERFSIETNLRRALERHELYLDYQAKMDFKSGTITGVEALLRWNSPQLGSITPT
                     QFIPVAEETGIIVPIGRWVLKTACVQNVAWQRQGLPPIRVAVNLSLRQLMDDKALEDI
                     KNALDESGMSPNLLELEITESMIMFKPERLIGVLTKIKEMGVRLALDDFGTGYSSLSQ
                     IKHLPIDTLKVDRSFIRNLPHDSDDKAIIEAIIAMGKTLSLNIVAEGVETLEQDHFLR
                     EKICDEMQGFYFSKPVAPDQFADLLRQYNFPS"
     gene            12533..13588
                     /locus_tag="PLG98_07685"
     CDS             12533..13588
                     /locus_tag="PLG98_07685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE33173.1"
                     /translation="MRIKRFFIAFSSYVMICSLVILGYILGFSPVPLRVLVVSQLGIL
                     ACNIILYVMFRTGLNKRFKDPSLTLLQIVIATCWLLEVLYYADSVRSAVLMDFLIIFI
                     LGLFKFNVREFLFLSFFTVAGYTGVILLLYINRPESLNYKVEILNIAILATVLPWFSL
                     VGGYITRLRKKVALSLATVKESEKKYIELSIIDDLTQLFNSRHFYAQLEKEIERANRY
                     NQPLTLLMLDLDKFKNFNDTYGHLEGDQVLSRIGHVIKRCLRDSDSAYRYGGEEFVIL
                     LPMTKAEAGITTATRIQETLRKTSFSPVSDQEIFMTVSVGLSQYIPREDMKVFVRRVD
                     KIMYQAKHQGRDRICTG"
     gene            13779..13985
                     /locus_tag="PLG98_07690"
     CDS             13779..13985
                     /locus_tag="PLG98_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33174.1"
                     /translation="MTYKELLEKQIGRECTFTASHTKCVINFGAAEPFASEGIIENVT
                     DDYVIIKSQSSTMIYLLVNTSISY"
     gene            14227..15519
                     /locus_tag="PLG98_07695"
     CDS             14227..15519
                     /locus_tag="PLG98_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33175.1"
                     /translation="MTSFAYGNVVQESDAQVWKVGRHRWTIHDEYRYGIWIEESVTED
                     FFIRHNIRVDCADVPYAMRWIYARIHHLPAAASTVDNRFIGHWSKDWSHLPTNPHWHK
                     DRRFRAALMSVLSSTSTQTLPSDTYPIKIGRHSVTPGTVFLFARDHAGIVSHVVMDGS
                     TTHPVQTFEASSPARLQGLRLKDFLLPNPNADYISGLLKFRWPVSDGNTWRYLPLEEQ
                     PFYSDEQYLPAFTKGYSNYLEAVEKRINPAVYEPGEKAEKIMMTLYRRLNERVPIVLK
                     GYIKCHGIECPEGSLLWEIYSTYNRDDFIGFLLHYLEQIIARNHLDRNDVLHKMEGIR
                     LQITPGRFVTLRHVLENFKWIASDPEAGIEARWGLDKCSTIATRLRTAEESIVFIQNK
                     YGETDPLFAEQSILKQQKIVDEMTSESLENNCMKNALR"
     gene            complement(15580..17109)
                     /gene="guaA"
                     /locus_tag="PLG98_07700"
     CDS             complement(15580..17109)
                     /gene="guaA"
                     /locus_tag="PLG98_07700"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOE33176.1"
                     /translation="MILIIDFGSQYNQLIARRVRENHVYCQIEPPGISIAAIREMEPE
                     GIILSGGPASIYSKGAPRVDKKIFKLGIPVLGICYGLQYMIDSLGGKVISSPKREYGF
                     AELLVKTQKGIFRGVARKTPCWMSHGDSIGELPKGFAVTATTANTAVAAAQDVRRHFY
                     GLQFHPEVVHTRDGRIMLANFLFGICRCKKTWSMQSFVQEAVNDIRRQVGREKVLLGL
                     SGGVDSSVAAVLLHKAIGKQLTCVFVDNGVLREGEAQRVIGMFKKHLQINLRFARAGK
                     IFLKKLKGVTDPERKRKIIGRTFIEVFNREARKIKNVKFLAQGTLYPDLIESCSAFGG
                     PSAVIKSHHNVGGLPKTMSLQLIEPLKHLFKDEVRLLGKELGLADDVVWRQPFPGPGL
                     AIRIIGEVTPQRLSILRKADTILWEEIRARKLYYKLWQSFAVLLPIKSVGIMGDQRTY
                     ENILSIRAVTSDDAMTADWAPLPHDVLARISNRIINEVRGVNRVVYDISSKPPSTIEW
                     E"
     gene            complement(17375..>18351)
                     /locus_tag="PLG98_07705"
     CDS             complement(17375..>18351)
                     /locus_tag="PLG98_07705"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HOE33177.1"
                     /translation="RLGANEAPPAIISVFLGEKLTQILENIQRGKVTKATNADLIDLG
                     IFSLPTLSKDNTDRNRTSPFAFTGDKFEFRAVGSSQNISFPAMVINTIVAESLDELAE
                     KIKTKNSKNINQAVLEVLKEEISYSKAVLFNGDNYSREWEIEAARRGLPNEKTTPTAL
                     KALTTVKALNLFEKYQVLSNPELKSRQLIHVDRYIKDLEIEVKCLNTLCTSQIIPAAV
                     VYQKKLADAINSTRQALGNAASVTAEIELLKKITDLINSIHATNKEIQVKIEAANALH
                     DEQKKAEMLSAKVKPMMEELREYADALEILVDDDIWPVPKFWEMLFIS"
BASE COUNT         4427 a         4652 c         4816 g         4456 t
ORIGIN      
        1 taaaaccggt ttgtgacctc tttttttagg gcactgtgat aaaattgtac attcattttt
       61 ctggtttgat catttcagcc agaatcgctt cctgcgtttc cgtgtcttcg ggtatagcat
      121 caattttttt caggatagga tcatcggccc taaacatatt tgtggtttcc gtctcgtcat
      181 caaaaacaag cacaaccgat ccattcagca aggaggcttt tgcctgcttg aattttgtct
      241 ccatggaagc atcaacagcg ccatagtccg tcccggctct ggatataaat tcttctatga
      301 cgcctcttaa ggctctcgca ctgagtttat tgactggtat aatatggatc atttcttctc
      361 aacaccgttc agtcattaaa atgataatgc tccaccgttg attccagcag gtttatgaaa
      421 taattgacat cattcaggca aagattacac ttttcatgtt caattaactt aacatgttcc
      481 ctgaaaaatc tgttaacaaa tattttatat tttgtctgcg ccacttcggg aatcaaagga
      541 tatccggaaa tctgcatccg gtcaatgccc cgacggttct gcttctccga aaaatctggc
      601 atattcatat tgccagtggt tcgtccaaca tcatgcgacg tgcccatgtc ggtgaggcct
      661 tgctttgcat tcataaaaca tggtagtgat tgacacagca ttccatcatc aaatgatgat
      721 ctgtgttaat tgttttgttt tgaaaaaaat ttgaaagttc aacattatgg gccttcatgt
      781 cgtcttttcc aatcgtcttg aaattctgaa ggatatcctc cttgaggacc tttcccgtat
      841 tcctgccgat ccgtttgaag cgcaacatgt cgttgtgccg ggaacggcga tatcgcgcta
      901 tcttcaactg gccattgctg acgaaaaagg catctgcgcc aatgtggcgt tcgattaccc
      961 cggcagctgg ctgtggaaac tggcaaggct tgtcgatgac aaggtgccgg aacggtctgc
     1021 cgttgatcct gaaatcatga cgtggatgat cctccgaatc ctcggcgaca accggttagc
     1081 cgcctacccg cgcctggccg ggttcattca aaaagcggat gatctgatgc tttttgaatt
     1141 ggcccggtcc gtggcgcatg tgtttgatca ttacgcgacg tatcgtccgg attggctggt
     1201 ggcatggagt gaaggaaaaa gaataaagga tttccgggag gagtgcgcgg atgaagcgaa
     1261 ggtttacgag gaatggcaga tggaaatctg gcgtaccgtc acacaagagc tcaagctggg
     1321 cagcacccac ccgctgaaaa tatttcttga taaaatcatg acgggcaaaa caccgggagc
     1381 agcatcggac ctgcccggca gggcggcgat ttttgcgctg cctgtcatcc cgccgattta
     1441 ccttcagacc atccgcaggc tttcggaggt catggacatt accttttacc tgctcaatcc
     1501 ctgccgggaa tactggtttg atatcgtacc gcccaaacag cttgcctatc ttaaaaagat
     1561 cggacgggac gcgcacacgg aggtcggcaa tcctctgctg gctgactggg gccggtcaac
     1621 gcaatcggcc attgatctga tatatgatga cgcgtcgtca gcaaacacac tggagacgag
     1681 tcgttttatc gagccggagg gtcaggcgct gctcgcgcaa ctgcagaggt ccattttcaa
     1741 catgcaggag ctggatcccg gcagcgtgca gttgccgcac aacgaccgga gtgtggaaat
     1801 ccactgttgt cacggcaccg tacgggaact ggaggttctg cacgacaggt tgctgtcatt
     1861 gttttgtgca gaccagacac tgagcgtgga tgatgtcgtc gtgctcacgc cggacatcga
     1921 tgaactgggc ccggccgtcg atgcggtttt cgggacagtg cccaaggatc gtttcattcc
     1981 ctacatcata tccggcaggg caatggccgg aaccaatccc tacctgaaca tgcttatggg
     2041 actgcttgat ctgacggcct cgcggatgcc ggccagcctt gtctttgatc ttctgagcca
     2101 gccgctggtc gccagccgct tcggggtgga caatgaccgt ctcaacagca tccggagctg
     2161 gctgaacggt tccgggatct tctgggggat tgacggcgaa cacaggcggg aactcggcct
     2221 ggcggacgaa gacaggcata cattccgcag aggcctggat tcgatgctgc tcggggttgc
     2281 cattccgtgc cttgatgaac cgattgccgg ctattttcct tatgaggact tggagggatc
     2341 gcgggcggag acactgggtc aattatggct ttttgtggaa aggctggcat tttggaaaaa
     2401 ccggctgaaa aatccgctgc cggccaatga ttggcaggac cttctcaacc ggatgctcgc
     2461 cgattttacc gaatgcggaa gagatgccca gggggcgtat gaccaacttg tcggcgccat
     2521 tgctgaactg gcagataact ggcgtgcggc aaacctgaca aaggcaattt ccagccgtgt
     2581 gatcaaggcc gcgctggccg atgcggacgt tagaagacgc ggggcggtgc cgtccggaac
     2641 ggttaccttt gcctcgctgt cggcgatgcg cggcatccgg taccgggtgg tttgtcttgt
     2701 gggcatgaat gacggttcgt tcccagggcg cgcatattct ccggaattcg atcttatccc
     2761 gaaggcagaa ccaagacgcg gcgaccggca aagaaatctt gaggagcggg gcattttcct
     2821 ggacgccctt ctggctgccg gcgagattct gcatatttcc tacaccggcc gcgaccagcg
     2881 cgacaacgcc ataatgccgc cgtccgtcct ggtcgcgaaa cttctggatt acctcagcct
     2941 ggcgatggca ccggaaaacg ccacagccaa cgatctggca acagcccgca ggcagctgac
     3001 ggtggatcac ccgttgcagc ctttttcgag gcggtatttt gatcattccg atccgcggct
     3061 tttcagctat gtcggtcagt atgcaacggc cttgaatcgt ggcagcaagt cgacaccggt
     3121 gaaaaagaaa gcggatttaa gaagtgatga gcaagaggag gatatgcctg atccgaagac
     3181 acctttcttc aacggttatg gggcgcctcc caaaagcccg gatacggagt cgacaacgtt
     3241 aaccgtggac gacctgtcct acttcctgat gaatccgagc agatttttcc tgcgcagaca
     3301 gctccggata cagcttccac aggaagagga tgttatcgcc gatgaagaac cgctcctctt
     3361 tgaattttca gaggaacgtg atgcggcggc gatgattatc gaggcatgcc ggaaacagaa
     3421 gcgcgtcatc acaattcagg atgccctggc tatcctgcag gcttccccga gttcgcctcc
     3481 gggaagtgcc tgcgaggccg ccctgtcagg catctggccg aaagtgtcgt tgcttgccga
     3541 aaggttgctt tcggcaacaa ccggggaaaa attgcagccg gtttattcct tgctatccat
     3601 tgatgttgaa ggagaaacct ggcaactcaa tgccgatctg atggaactcc gtctttgcgg
     3661 ccttgtcttt taccggtgcg acgatctcag ggatttcgac catctgaatg cctgggtccg
     3721 gcatctggtt ttatgcgtct gccggcccga gggtgtcttg ccgcagacca ggcacgttgc
     3781 gcttgacggc gatttaatct tcggggatat tgcctgggaa caggccgcaa agcatctcgg
     3841 caacctgctg gcgctctaca gagacggact gttcgagcca gtgccctttt tcagaaaatc
     3901 agcatggaaa ttcgtgcaaa aggatatgaa cgcggcaaag agcaagtggt ccggagggtt
     3961 caatgccggc ggtaaagcgg agagtgctga tccctggcat gctctggcat ggcggggact
     4021 ggaggcggac gatgttctga atgaacgctt taaggaaatt gcacactgtg tttttgcgcc
     4081 catcctcgcc aatgaaaaat tagaaaaaat tgtcaccgaa gaagaaaaag gggaaaagcg
     4141 tggccggaaa aaataaaaag gtggaacctc tggatgtttt cacatgcgat cttaacggga
     4201 tcgccaacat cgaggcatcg gcaggaacgg gaaagacgtg ggccatctgc ggactttatg
     4261 tgcgcctgat tgccgagatg gatctcccgg tggatcagat cctcgtggtg accttcacca
     4321 atgcggccac tgccgaactg cgcagccgta tccgcgacag gatggtggaa gtgcagcatt
     4381 gcctcgatac tggccgctgc ctgtctccgg acccgtttgc cgcaggtttt ctcagcagaa
     4441 tggaccggct cgggatcagg gccaaggccg gagaaaattt gaaaaatgcc ttgttgtcct
     4501 tcgaggaatc cgcgatcttt accattcatt ccttctgtca gcgcaccctc ggtgagcttc
     4561 ctttctcggc agtccagccg ttcaacgtcg aggtggaggc ggacgattcg gagatcatcc
     4621 gggaaacggc ggcggattac tggcggtgca tgacgcaaca ctacacggcg gatgaaatca
     4681 acgcgctgct gcaaaataag ataaacccgt catggattgc cggtgaaata cggcgcctgc
     4741 ttaacaggcc gctggcaaag ctggtcatgc ccgaaacaaa tccctcagaa aatccggcgt
     4801 cacgaagggc ggtctggatt aaaagattca ttttgaaaac ggcgccccgc gccatccgca
     4861 aaaccaagca ggatcgccat gtgatttcct acaatgacat gctttataat ttgcatgacg
     4921 ttctcacctt gggacaattc ccatggctgg ccggagtatt gagaaaacgc tttccggtgg
     4981 ccctgatcga cgaattccag gataccgacc cgctgcagct tgaaattttc cgcgccattt
     5041 actttgacaa aggaaccctt ttcatggtgg gcgatccgaa acaagcgatc tattctttcc
     5101 gcaacgccga cctgaacaca tacctcgccg ctcggcgtca ggcccagcgt caatggacca
     5161 tgatccataa tcagcgatcg gaagaaaagc tgatctttgc catcaatgcg gtattcggcg
     5221 caaacaagaa cgtctttggc gatggtgaaa agattccgta ccgcgacgtc aggaggggca
     5281 ccaagcccct gaaggaactt cagggggatc ctgaaccgcg cgaacctttt tccgtctggc
     5341 ttttcaagac cgggtcgaaa gcagaacccc ttaaagcggg ggacgcccgg tcgcttgcac
     5401 tcgctgcaac ggcaaaggaa atggcccgac tgatcgacgg aggaatgcgg ggcaccatcc
     5461 agataggcga tgagaacttg cagccgcgtg atatcgctgt tctcgtgcgc acaaaggcgc
     5521 agggaagaca ggttaaagag gcacttgcgg attatggcat cggcagtgtg gatctgaccg
     5581 aacgcagtgt ttttgcaacc gttgaagctg aagaaatgaa gaccgtcctg cgggcgatcg
     5641 ccgagccatc tcaagccggg cttttgcggg cggcgttgtc cacgatgctg cttggattcg
     5701 gcagcaggga gctggccgat ctgaatgccg atgagaccga atttaaccgg attgtcggac
     5761 aggtgaatga ataccggcag gcatggctcg gcagcggttt cggcgccatg tggcgcaggc
     5821 tgattgcaca ggagagggtt atcgaacggc ttttgccgct gaagaacggg gagagaaaaa
     5881 taaccaacct gatgcacctg atggaacttg caacaggtgc agaggagacg caccgcggca
     5941 ttgaagcgct tttgcgctgg ctgagtgaaa agcgttccgc cggggacgag gaggatgtgg
     6001 cgcagctgag gctggaaagc gacgaaaatc ttgttcaggt cgtcaccaaa cacaaggcca
     6061 aaggcctgga atggccgatt gtcttttgcc cgttcgtctg gagcgaccgc agccggtcat
     6121 tgaaggatga ggtctgcacc tatcacgagg gcgataccct ggtgcttgat tatgcaaccg
     6181 acgacgaaaa aaagaaaaaa caggaagacg aggccggcga agaacgggtg cgactgattt
     6241 atgttgcgct gacgagggcg gcaaaccgct gctatattgt cgctggtcct ttctggcaac
     6301 cgtcgggaaa gggtgcaacg gaaaaaaagg cgaagaagag catcctgaac tggatcgtcg
     6361 ccggcgatga gataaaacac ggcgtgtggg cggaaaaaaa agaggtgacc gttgattcca
     6421 tgcatcaggc atggcagaga ctcctgacat caaacaaagg gatcggaggg attccgtggc
     6481 cggataaaaa agagacagtc cgtccggtta tttgcaaaga gaagagaaaa tatgaagccc
     6541 ggaaggtcgg cagactgccg ggtgtggcct ggaaaatatc cagttactca tcgcttaccg
     6601 cctcctccct tgaaggagga aggcaacacg aaccggcgac acaggattat gacgtcatcc
     6661 cccttgcgat tgaagatcat gatccgcctc aagtcctccg ggcaaagctt tcgggcgata
     6721 tcatcgattt tccggccggt accacaacag gacattgtct gcatgccgtt ttcgaaagga
     6781 ttgactttca ggacgaaagc ggatggaaag acatcatcac caacgtgctg gcgaattatc
     6841 cgccggtgga tgccttagaa aagcgcgatg atcaggtcag ccggattcac ggtatgctga
     6901 aagatgtcat gagcacccct ctgccaaccg ggtttaaact cggcgacgtt gccagggcaa
     6961 aaagattttc cgaattcgag ttcgcctatt cggtacgtca ccttgatcac gcaaaactta
     7021 agaatcttct cgacaaacac ggctgccgga ttcccccgct ggattttccc gatctcgaag
     7081 gttacctgcg gggtttcatt gatcttgtct ttgaacacgc gggcaaatgg tacgtactgg
     7141 attggaaatc gaacaagctg ggcatcagtg ccgcggagta ttcccggtcg tccgtcgatg
     7201 aggcaatgtt caatcatgcc tatgaatttc agggcctgat ctatcttatc gctcttcatc
     7261 gttacttgaa actgcgtatc agcaaatatg attacagccg gcacatggga ggcattctgt
     7321 atctctttgt ccgtggcgtt cgtccggcat ggccttcggc aggtatctgg cataaactgc
     7381 ccgagccggc gctgatcgaa tcactggatg ccctgttctc ttcagaaaac cggaggaccg
     7441 tatagcgttg atgagaaaac atgaaaacat acctgcggcc gattatgcat cgcccctgtc
     7501 caggggactt gcggattttc tgaagcgcct ttctttgacc cttggtgcta cggaagaggc
     7561 ggcgcgcaca gcgggcgatg ccgccagggc gtgcgctcaa gccctggccg aaggcgatgt
     7621 ctgcgcgaat ttctctctgc tggccgcagc ggaaggcaag ccggcacagg atgtacgaaa
     7681 gctgctggat gcttctttgg tggcctcatc cgccggggcg gataaaaaat tgcccctcgt
     7741 gtatgacgat gaaggacaaa tctacctgta ccggtacttt gcctatgagc ggaaactggc
     7801 cgatgcaatt atcaggcggt taccggggcc cgcggcgcgg acagtcagcg ccgaatcgcg
     7861 acagtttctc gaaaaacggt ttgcggaaaa tgcaagggaa ctatccggcc ggccggactg
     7921 gcagaaaatt gcggtagaaa tggcattcac gaaccgcctg acgatcatca gcggaggacc
     7981 cggtaccggc aaaacaacca tcgtaacagc cctcatcgcg gcactggcga taaatgatca
     8041 tcccccgcga atcgcgcttg tcgcgccgac cggcaaagcg gcggcaagaa tggaagagtc
     8101 gatgcgcagt cagatgagca cacttgacgc ggagttaagg aaacgcatgc ccgagcgggc
     8161 ctccaccatt catatgctgc tgggagccag cccggctggc aatacgttcc gctttaacga
     8221 aaacaacccc ctgccctatg atttgattgt cgtcgatgaa gcatccatga ttgatttatc
     8281 cttggcctca aggctgtttt ccgcactgca tccggatact ggaattgttt tgctggggga
     8341 taaggatcag ttggcggcgg tcgaagccgg agcggtattc gcagaactgg cgcagcagac
     8401 atcggttcct gacaatatgg acaagctttc gacaggaagc ggcacaccac ctctcaacgc
     8461 tcaaccaaac gagaaagggc cgttgtccag ttgtgttgtc tggctcaagg agaattaccg
     8521 gttcagcgca gcgtcgccca tagctaaagt ggcgggcctt gtcgttaacg gtgaggggga
     8581 acaactcatc gaatggcttc aaaatgaaaa aggggataac atcaagtggg aagcctttga
     8641 tgaaggactg ccgtcgcgtg ttgtaggggc tcttgtcaag gggtttgaac cgtttgttga
     8701 ggcggtaaat cagggcgatc cggagaaggt gcttgccgct tacgaaaaat tctgcgtcct
     8761 ttgcgtcatc cgtaacggca gacggggtgt caacggtatc aatgacgtca tagcgcggca
     8821 gttacgcctg aagatatcgg gagaaggacc tggtgagggg ccttggtatg acggtcgtcc
     8881 gattatgatt acggagaatg attatggcct tggggtattc aacggcgaca tcggtgtggc
     8941 cctcaagacg aaagacggtc atcgtcgcgt ctggttcatg gacaaagacg gagggttgcg
     9001 ttttgtgcct ccttcttcct tgccggctca tcaaacggct ttcgccatga cggtgcataa
     9061 agcccaaggg tcagaattcg aaaatgtctc gcttattctg cccgagcagg acacccccgt
     9121 gttgaccagg gaacttatct atacggcggt tacccgcgcg aagaagactc ttcttatata
     9181 cggcagcgcg gacattctga agctggccgt aggccgtcca acatcgcgtc gcagcggcct
     9241 ggcgaaaaaa attagtacgc caccatgacc gccgacacgg accgaatccg gcaatctttc
     9301 aacgcccccc cctctgtaga acacaaccca agccgttttt tcaaaccccg gcgcgtaaat
     9361 atttttggag agacggctga gagtttttct tcggctaatg ttcaggaaat ttttttatga
     9421 agggaaattg tactggcgca gcagatcggc aaactgatcc ggtgcaacag gcttgctgaa
     9481 ataaaatccc tgcatttcgt cgcagatttt ctcccgcagg aaatgatcct gttccagtgt
     9541 ctccacacct tcggcaacaa tattaaggct cagcgtcttg cccatggcaa tgatggcttc
     9601 aatgatcgcc ttgtcgtcgg aatcatgagg aagattacgg atgaacgatc tgtccacctt
     9661 gagggtatcg atagggagat gcttgatctg ggaaagggat gaatagccgg tgccgaaatc
     9721 atcaagggca aggcgcacac ccatctcctt tattttggtc agcacaccga tcaggcgttc
     9781 gggtttgaac atgatcatgc tttccgtgat ttccagctcc agaagattcg gcgacatacc
     9841 ggattcgtcc aaagcatttt tgatatcttc tagagcctta tcatccatca actgacgaag
     9901 cgataaattg accgccaccc ggatgggcgg caggccctgg cgctgccagg cgacgttctg
     9961 tacgcaggcg gtttttaaga cccaccggcc gatgggcacg atgatgccgg tttcttcggc
    10021 gaccggaata aattgggtcg gcgtgatgga ccccagctgc gggctgttcc agcgcagcag
    10081 ggcttccaca cccgtgatcg tgccggattt gaagtccatc ttggcctgat aatccagata
    10141 caattcgtgg cgttccagcg cccggcgcag attggtttcg atggaaaaac gctcgtagga
    10201 ctgcgactga atgttctgtg aataaaactg gtagttgttc ttcccttctt ccttggcaaa
    10261 gtacatggcc atatccgctt tcttcatcag ggtctgttca tcctctccat ccgtagggta
    10321 cacactgatg ccgatgctcg atgtgacgcg gcattcttca cccagcagaa ccatcggttt
    10381 catgacggtg ccgaggattt tctgggcgac gatggcgacg ttggccaggt cggttacatc
    10441 ctcgatgagg atgatgaatt catcgcctcc cagacgtccg acaacatctt ctttatcctt
    10501 ggggcgtccg atcacgtcag cggcgcgcag ggtttgccga aatcgtttgg ccatctcttt
    10561 caatagtctg tcgccggcat catgccccat ggtgtcattg atgattttga accggtcgag
    10621 atcaataaag agaagcgcca gctgacgctt gttgcgtttg gcggacttaa tggcctgatt
    10681 cagcaaatgg ttgaacatca tgcggttggg caactgggtc agattatcgt gtgttgccag
    10741 gtattggatc tgcgcctcgg cgcgcttacg ttcggtcaca tcgcggcttt cgcccaggat
    10801 ggataaaggt ttcccatttt tatcctgaat gaaacttaac gtggtatcga gcgatatgag
    10861 acgaccgtcc ttgcagcgaa attcaacttc caaggaacgc ttatagtcgg gcgggggggg
    10921 gacaatctcc gccttggcca tttcctctga aaacatttcc agcgcttttt ggaaagattc
    10981 ttcggtgaga agtttatcca gggagatctc tttaatctcg gtaaacgtgt atcctgatgc
    11041 cttctccgaa gacgggctga aatattgaaa cttcaagttc agatcgaaga gccacacctg
    11101 gtcggtcata tgatccgcca gaagacggta ttgttcctcg gcacgcttgc gctcggaaat
    11161 atcccggctg acgcccagaa ccccggtgat gtattgttcc ttgtctctga ccagggaaaa
    11221 cttggtttcc gtccacacgg gatttccgtc tttccggttg atttccagga gcagggttct
    11281 ctcaggggaa tcctggcttt tggatttgcc catctccatc atttcggaca acgtttccat
    11341 cgcggtgtcc agggaggtgg gcgcaaagga atcggcaaga ttctgtttca ttacgtcttc
    11401 cggttcgtag cctctcaggg ttttgaccga cggactgaca taggtgtaga tcagattcat
    11461 atccaccacg aaaataacat ctttgatgtt gtccgtcagc cgctcgatgg tcgtaagggc
    11521 attggttatg ctggcgcgga gtccgttgat gtatccgccg atcatggcaa accacggcag
    11581 aataatggcc aggacgataa tgttcaataa atccagtttg ggattgatca attccgggtg
    11641 cagcttgtaa acaagaaaaa taacagccga atagttaacc aacgcgtata acgacaacag
    11701 caggaactgc cggacgcgca accggaaaaa tccgaacacg agcacaacca tgtaaacgag
    11761 aaggcacgcg cctctggcct cgtaggcata gtacatgacc accataatcc agaaggtggc
    11821 aatcaccatt tgaagaagag tcagactcgg atctttggct tttttattga atccggttct
    11881 gaacacggca tacagaagaa tattgacaac aatatttgcg gtgactccgg caaccaggat
    11941 ggataacggc acttttgtca gtcccagtaa ataactggca atggtaatgg cgttccacat
    12001 cacatacgcg ccaaaagcga tgaggaagcg cttgattctt aaagtctgat tgtagtcatt
    12061 ttcaggaaag atattgatca tgaatatttt tttattaccg caacatctcc ctttctggat
    12121 tgaaacaaat atactcaaaa aaagttgtta agtaaatcat ttaatagacc cggattgcct
    12181 cgccctccct cggccaatcg ggattgcagg gggcccatcg catttccggt gatcaacaga
    12241 cggatcacaa acgacgcctg ttgttgagca ttgctaaatt ccaggctgat catcattgac
    12301 atattcagaa cttggtaata taaatgcgca tcaaaaaaat tttcaggggt gtgaacatcg
    12361 gtgcatgccg gtccggcacg cgacttgcga acaaccaggg ggcgttgaaa cggattgcag
    12421 taagacactc tttttttgaa ataatccgga cagtcggcaa acacaacgaa ccggcttata
    12481 aaggagaata tatatcttca gtataattcc caaagacgat cgcaagcagg cgttgagaat
    12541 caaaagattt ttcatcgcct tctcatcgta cgtcatgatt tgttctctgg tgatcctcgg
    12601 ctatattctg ggattttccc ccgttccttt gcgcgtactg gttgtttccc agctggggat
    12661 tctggcctgt aatattatat tatatgtaat gttcaggacc ggattgaaca aaagattcaa
    12721 ggatcccagt ctgacgcttc tccagatcgt aatcgccacg tgctggcttt tggaggtttt
    12781 gtattacgcc gattccgtta gaagcgcggt gttgatggac tttctgatta tttttattct
    12841 gggccttttc aagtttaatg tcagggaatt tctttttctc tcgtttttca ccgttgccgg
    12901 ctacaccggc gttattttgc tgctttatat caatcgtccc gaatccctta attataaggt
    12961 agaaatattg aatattgcca tcctggcaac tgttttgccc tggttttcac tggtcggcgg
    13021 atacatcacc cggctcagaa aaaaggtggc cctctctctt gccaccgtca aagaaagcga
    13081 aaaaaaatat atcgagctga gcatcattga cgatctgacg caacttttca attccagaca
    13141 tttttacgct caactggaaa aggaaattga aagagccaat cgttacaacc agccgttgac
    13201 cctgctgatg ctggatctcg ataaattcaa gaatttcaac gacacctatg ggcaccttga
    13261 gggcgatcag gtgttatcca ggatcggtca tgtcatcaaa cgatgcctgc gcgattccga
    13321 ttccgcctat cggtacggcg gcgaagaatt cgtaattcta ctgccgatga caaaagcgga
    13381 ggctggaata acaacagcaa caagaatcca ggaaacgctt agaaaaactt cgttttcccc
    13441 cgtgtcggat caggaaattt ttatgacagt aagcgtcggt ctctcgcagt acatccccag
    13501 agaggatatg aaagtctttg ttcgccgggt ggacaagatc atgtaccagg caaaacatca
    13561 gggcagagac agaatttgca caggctagag aatcagaggt cgccccttaa aaggcgcgta
    13621 tcaagagaaa aaatccctat tttattcttt aaaaaaattg gaatcgtgct attataatta
    13681 cgaataatgg ttgacggtgt aatgctcttg atgaagagga ctttcgaaaa gccatcaacg
    13741 tgtgacgaaa ataacttttt gaagaaagga atcgcacaat gacttacaaa gaactcttgg
    13801 agaagcagat tggaagggaa tgtacgttta ccgcaagcca taccaagtgc gtaattaatt
    13861 tcggagcggc agaacctttt gccagcgagg gaatcatcga gaatgttacc gatgactatg
    13921 tcattatcaa aagtcaaagt tcaactatga tttatcttct ggtcaatacc tccatatcgt
    13981 attaacacaa atcacaaacg gacaaaaatc agataccgac ggtaatttcc cccgttctat
    14041 ttttaccggt tttgccatgt ttcccgaata tgactttcac cgtcgcaggg caaaaacgcg
    14101 gttgatgttt ttcttgtata aaaaataatt gatggtatgg ttttcatgat tttcggagtt
    14161 ctgcggaccg gggtgataat gttttttctt cgctgcatac tgatatgctc cgcggcctgt
    14221 acgcttatga catcatttgc atacggtaat gttgttcagg aatcggatgc tcaggtatgg
    14281 aaagtcggcc ggcaccggtg gacgattcat gacgaatacc gatacggcat ctggatagaa
    14341 gaaagcgtta cggaggattt tttcattcgc cataatattc gcgtggactg tgccgacgtt
    14401 ccctatgcca tgcggtggat ctatgcacgc atccatcatc tcccggcagc cgcaagtaca
    14461 gttgataacc ggttcatcgg acactggtcc aaagactgga gccatctacc gacaaatccg
    14521 cactggcata aagacagacg tttccgggcg gcattaatga gcgtattgtc atcaacctcc
    14581 acacagaccc tgccgtccga tacgtatccg ataaaaatcg gccggcactc cgtaacaccg
    14641 ggaaccgttt tcctttttgc cagagatcat gccggtattg tgagccatgt cgtcatggac
    14701 gggagcacca cccatccggt ccaaaccttt gaagccagtt caccggcaag gcttcaggga
    14761 ttaaggctca aggattttct cttgcccaat ccgaatgccg attatatttc cggcttactg
    14821 aagttccgct ggccggtctc ggacggcaac acgtggcggt accttccgct ggaggagcag
    14881 ccgttctatt ccgatgaaca gtacctgccg gctttcacga aaggatattc caattatctc
    14941 gaagcggtgg aaaagcgaat caacccggca gtctatgagc ccggcgaaaa ggcggaaaaa
    15001 ataatgatga cgctctatcg ccggttaaat gaacgtgtcc cgattgttct taaaggttat
    15061 ataaaatgtc atggcataga atgtccggaa ggctctcttt tgtgggaaat ttacagcacg
    15121 tacaatcggg atgactttat cggttttctg ttgcactacc ttgaacaaat tattgccagg
    15181 aatcacctgg acagaaatga cgtgcttcat aaaatggagg gcattcgcct gcaaattact
    15241 cccggccgct ttgtgacttt aagacacgtg ctggaaaact tcaaatggat tgcttccgat
    15301 cccgaagccg gtattgaagc ccgatgggga ctggataaat gcagcaccat tgcgacccgt
    15361 ctccgaaccg cagaggagtc tatcgtgttt attcagaaca aatacggcga gacggatcct
    15421 ctgtttgccg aacagtccat cctgaaacaa cagaaaatcg ttgacgaaat gaccagtgaa
    15481 agcctggaaa acaactgcat gaaaaacgca cttcgataat gattgtctcg cccgctccaa
    15541 aaatctattt tactgcgagc gtcccggcat taccgcgacc tattcccatt cgatggtgct
    15601 gggcggtttg gaactgatgt cgtaaacaac acgattgacg ccgcggactt cgttgataat
    15661 ccggttggat atccgggcca gaacatcgtg cggcagcggc gcccagtcgg ccgtcatggc
    15721 gtcgtcgctg gtgaccgcgc gaatcgacaa aatgttttca taggtgcgct gatcgcccat
    15781 aatgccgacg ctcttgatcg gcagaagaac ggcaaaggac tgccacagtt tgtaatacaa
    15841 ctttctggcg cggatttctt cccagagaat cgtatcggct tttcgcaaaa tcgacaggcg
    15901 ctgcggcgtc acctcaccga taatccggat ggccagaccc ggcccgggaa acggctgccg
    15961 ccagacgaca tcgtccgcca gacccagttc ttttcccaaa agccttactt catccttgaa
    16021 taaatgcttc agcggttcta tcagttgcag gctcatggtt ttgggaaggc cgccgacatt
    16081 atgatgcgat ttgatcacgg cgctgggacc gccgaacgcc gaacacgatt cgattaaatc
    16141 cggatacagg gttccttgag ccagaaattt cacatttttg attttgcgtg cttcgcgatt
    16201 aaatacctcg atgaaggtac ggccgatgat tttgcgtttc ctttccgggt ccgtcacgcc
    16261 cttcagtttt ttcagaaata ttttgccggc ccgtgcaaag cgcagattga tctgcaaatg
    16321 ttttttgaac atcccgatca cgcgctgcgc ttccccttct cgcaagacac cgttgtccac
    16381 aaaaacacag gtcagttgtt tgccgatcgc cttatgcaac agcacggcag caacggacga
    16441 atccacgccg ccggaaagtc cgagaaggac tttttcacgg cctacctgcc gccggatgtc
    16501 gtttacggct tcctggacaa aggactgcat ggaccaggtt tttttacacc ggcagattcc
    16561 gaaaagaaaa ttggccagca tgattctgcc atcccgggta tgaacaacct ccggatgaaa
    16621 ctgcaggccg taaaaatgcc gccgaacatc ttgcgccgcc gccaccgccg tattggccgt
    16681 ggttgccgtc acggcaaatc ctttgggcag ctctccgatg gaatcgccgt gactcatcca
    16741 gcagggagtt ttccgggcaa cgcccctgaa aatacctttt tgtgttttga ccagaagctc
    16801 ggcaaatccg tattcgcgtt tcggagagct gatcaccttc ccccccagag agtcgatcat
    16861 gtattgcaga ccgtaacaga tgcccagcac gggaataccc agtttgaaga tttttttatc
    16921 cacccgcggc gcgcctttgc tgtagatgct tgccggtccg cccgataaaa ttattccctc
    16981 cggttccatc tcccggatgg cggctatgga aattccgggc ggttcaatct ggcagtacac
    17041 atgattttcc ctgacccggc gggcaataag ctggttgtat tgagatccga aatcgatgat
    17101 cagaatcacg ttgtcttctc cttctcctgt tccttggttt tcatatacta cgcatacgac
    17161 tatttttgcc tgcgggctcc ggatgccgct gatgcatgat cccgcgcgcc aaaggcctgc
    17221 ggcaccggcc tgatattccg cagccgcctc aaaagcggca gccgcattgc cgtatgcgcc
    17281 gaaggtgcgg ttgtctatct ttttttctcc gtttcgtcaa tgagcaaaga gccggggaat
    17341 tgtcgtccgg accgtttccg tcccatggca tctatcaact gataaacagc atttcccaga
    17401 atttcgggac aggccagata tcgtcatcaa ccaggatctc cagggcatcc gcatactcgc
    17461 gcagttcctc catcattggc ttgaccttgg cgctgagcat ctccgctttc ttctgctcat
    17521 catgcagagc attggccgcc tcgattttca cctgaatctc tttgttcgtt gcatggatgc
    17581 tgttgatcag atcggtgatt ttcttcaaca gctcaatctc cgccgttaca gaagcggcat
    17641 tccccagcgc ttgcctggtg ctgttgatcg catcagccaa ctttttctga tacaccacgg
    17701 cagccggaat gatctggctc gtacacagag tattgagaca tttaacttcg atttccaaat
    17761 ccttgatgta gcggtcaacg tgaatcaact gccgggactt gagctccgga ttggagagaa
    17821 cctgatattt ttcaaaaaga ttcaacgcct tgaccgtcgt cagggccttc agggcggtag
    17881 gcgtggtttt ttcgttcggc aggcccctcc gggccgcttc gatttcccat tccctgctgt
    17941 agttgtcgcc gttgaacaat accgctttcg aatagctgat ttcttctttc aaaacttcca
    18001 gaacagcctg attgatgttc ttggagttct tcgttttgat tttttcagcc agctcatcga
    18061 ggctttccgc cacgattgta ttaatgacca tcgccgggaa ggaaatgttc tgcgatgacc
    18121 ctacggcacg gaattcgaat ttatcgccag taaaggcaaa aggcgatgtc ctgttgcggt
    18181 cggtattgtc tttgctgagc gtcggcagtg agaaaatccc caaatcaatc agatccgcat
    18241 tggttgcctt ggtcactttt cccctctgga tattttccag aatctgcgtc agtttttccc
    18301 ccagaaacac ggagatgatg gccggcggcg cctcgttggc acccaggcgg t
//
LOCUS       DAOSME010000045        18221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3686_length_18221_cov_22.437411, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000045 DAOSME010000000
VERSION     DAOSME010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 18221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18221
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3686_length_18221_cov_22.437411"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLG98_07710"
     CDS             <1..225
                     /locus_tag="PLG98_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33178.1"
                     /translation="TVLGEQICKIIKKHLGINVEFGGNIAYDEHVHDAICQRVCFLDR
                     YPHTRAANDLRVLNRKMSNAFGEQLMLNYL"
     gene            247..900
                     /locus_tag="PLG98_07715"
     CDS             247..900
                     /locus_tag="PLG98_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE33179.1"
                     /translation="MEKDFDQMNYYEILDIGTNATSAEIRGAYNVALQMYQPDSLVSY
                     SFFSKGERDKILSLIDKAYFTLINEAQRAKYDNEMNLRETSVSADQHKAPPQPANIFE
                     FNRRDIASLTRKNNKSALKTKISQSQSIREILSRQKITGADLKAIREELGLAIETIHQ
                     ETKIRLDYLHDIEEDKTEKLPAPVFLKGFVKAYLRSLCIENADDISTAYMNTLPGKN"
     gene            1054..2055
                     /locus_tag="PLG98_07720"
     CDS             1054..2055
                     /locus_tag="PLG98_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOE33180.1"
                     /translation="MKKSLFYLLLISMLIFFVSACSDSDSDFDDTADAEYLTVQMRAD
                     AGNGEVSLDWQMLPRAETYNIYYMEDDEDDTNQPTSQKMKSEGTKIDGETNHIISAPY
                     VVTGLENGKTYWFALSAKNAEQAESDLSQAIYAIPRANPPLPAPKNVRANAGDKEATV
                     TWDAVTGAAGYIVYYYTSFANYQESPKITSTSYTFTGLTNKQTYLFFVAAVDSNDNNE
                     SGDSSASFVYTTTPSEDPPPSAPRNLRVINRGEGFVDLAWDAPLFHTGALTYNVYFFT
                     AGNITKSTAQKGIEALADTSTRAANLDKEKTHYFVVTAVDNNGESAESGEVSAIPDL"
     gene            2226..3452
                     /gene="gspF"
                     /locus_tag="PLG98_07725"
     CDS             2226..3452
                     /gene="gspF"
                     /locus_tag="PLG98_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOE33181.1"
                     /translation="MSVFEYIALDEKGRQLKGFIDASGISAARQKLREENIFPVEINQ
                     TETKKESALAGALKINLWERVSANQVAVFTRQLSTLLGAGMPLVPSLSILMQQEKNPL
                     LKKSLAQIREQVNEGKSLTEAMAEFPRIFPSFYLNMVRAGEASGTINLVLERLADFSE
                     NQQALMNKIRSALAYPIIMFIMGSAVIFLLMTFVVPKITGIFTDMNQTLPLVTIVLIK
                     VSNFLKAFWWIILILLFGGLAAIRYMTTGTDSGRRAWDNIRLKIPVMGQINRKIAIAR
                     FCRTLATLLQSGVPLLTAMEIVRNIVNNVIIGDAIHQAARDLEEGKGLSGPLTKSGLF
                     PPLVTEMIAVGEQSGTLETMLNRIAAAYENESQASITVMTSLLEPIMILFMGLVVGFI
                     VVSILLPIFEMNQLVR"
     gene            3481..3933
                     /gene="gspG"
                     /locus_tag="PLG98_07730"
     CDS             3481..3933
                     /gene="gspG"
                     /locus_tag="PLG98_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOE33182.1"
                     /translation="MKIFRRKKSRQAGFTLIELMVVIVILGVLAGMIIPRIMGRPDEA
                     RQAKARIQMEAMESALKLYKLDNGSYPSTEQGLKALVEAPTTGDLPKNWRQGGYLEKG
                     KVPKDPWGNDFVYLSPGSHGDFDLSCLGKDGEAGGEGVDQDINNWEVE"
     gene            3896..4447
                     /locus_tag="PLG98_07735"
     CDS             3896..4447
                     /locus_tag="PLG98_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE33183.1"
                     /translation="MSIRTSTTGKWSKQGFTLVELLLVITLIGIVLVLAVPSTRDVLT
                     GDMLKKTSRQLIGLERKLRTEAVRDQLDYILHLDLSDSVYWITASDMTEIKQAEIKKQ
                     ALHLPEDVRIIDVVDEDNRKITAGEVKIVFKKDNICSPRVIHLAYKEDKMTIVMNPFM
                     GVTDIYDQYVEIPFNTSEGQNRR"
     gene            4458..4829
                     /gene="gspI"
                     /locus_tag="PLG98_07740"
     CDS             4458..4829
                     /gene="gspI"
                     /locus_tag="PLG98_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOE33184.1"
                     /translation="MTRMTEKGFTLMEVMVAMAILAIALVSIFQLQSQSISMATDSRF
                     MTTAALLAQSKMVEVETQSPLSNKTENGDFGPDYPQYAWQLIIGDTQLPQFKKIEVTV
                     TNKLFLRRGTYTLILYKRPDM"
     gene            4832..5500
                     /locus_tag="PLG98_07745"
     CDS             4832..5500
                     /locus_tag="PLG98_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE33185.1"
                     /translation="MRHCSSRPHLSGGGFTLVEILIAIFILSLVLATVYVSYTGTLKT
                     SHQLEKEGEIYKMARQSLERLAKDLTSLQMSEGSFYLNAEKKKTGRHQFHNIFFWSAA
                     HLTFGELEGEGRPATIGYYVTENPDGKSFSLWRSDATGMKPDETKKTEDGFIICKNIE
                     TFHLTFYDSAGGENDTWETSAGGGMHQGKVPEAVKIELALVNDDDAQKPYKFMTRVFL
                     PVNK"
     gene            5730..6692
                     /gene="gspK"
                     /locus_tag="PLG98_07750"
     CDS             5730..6692
                     /gene="gspK"
                     /locus_tag="PLG98_07750"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HOE33186.1"
                     /translation="MFHNPRGVALITVILIIVILIAVVIELNRSSRADIYDAANLSDG
                     IKLSYIAKSGFYGAAALLNNSPGAYTSLRDDWAKMETVSLQSNTLFPEGYFIARIEDE
                     AGKIPLNKLVDGEEYNEEIRTMLDRLLAQPEFGLKDRQIDEIIDAIKDWIDTDENTTG
                     YGAESNYYESLDSPYQAKNAPFDCIEELLMVKGITKEIFYGTKDKPSLAKYVTADSEG
                     LININTAPKIVLRSLSDDISSELADRLDEYRMEKSHDVSGNQWYKQVPGMENITINQN
                     LITVKSTYFKIVSTGKISNMSRTVTGIVATGDQAQIAIITWRQD"
     gene            6692..8206
                     /gene="pilM"
                     /locus_tag="PLG98_07755"
     CDS             6692..8206
                     /gene="pilM"
                     /locus_tag="PLG98_07755"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOE33187.1"
                     /translation="MPNVLGLEIGRSTIKAVLMARKGLGGGRILDARILDVRDSEGIG
                     PALQKLAEDKNFAGIPCFVSVPSQDVLFRHVRLPFRDDNRIRKTLLFELEPLIPLPIE
                     NVIADYIALPREGLLVGAMPKETIREIIEQVEKNLGDVSVIDTSSSALAVQIPDHKKT
                     SVSIILDIGRNATTAAFCENESVIHIRSLAFSGEQITLAMAKDFSVDRNRAEQLKISG
                     DYPEAMEKAGEECRGLCSELKNTVEFMKINGVLHNDPAQILLTGGGALFGPLRKEIEK
                     NFTCPVELLNLISARQLEVEKNIMMRMNPSLLNAAAANALRSSAGKKSFNFRQGEFTA
                     RPSHLNFKNHLRWAGILAAVILLLAVVNQILDYSLKMRQIGGIKKDIARIFQKSFPDA
                     PSMVDPVQQLKTKIEEDKKTFGLGEGMPEATTADFLKELSALISPSLEIVFTALTYEN
                     RVISVKGEAKTVDDVTLIKDALAKSKSFKEVTMGPTSLTRDGGKVDFSLRIEVQ"
     gene            8203..8769
                     /gene="gspM"
                     /locus_tag="PLG98_07760"
     CDS             8203..8769
                     /gene="gspM"
                     /locus_tag="PLG98_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOE33188.1"
                     /translation="MIQELWNKLDATQRKLVAGAAVFVCIALILELAVFPFWEAKKKL
                     DRSISSNQKKLAEVKELSAEFNRLESKVSAMRRVASMRAANFTLFSHLEKKATQAGVR
                     NRIKNMNSSRGIQSASFEESLIDMKLEKVTIKQLVDFLYYAESPADLVRVKKIHIVKM
                     KDAPEYLSVQLQISSIQPSNQIPESGQP"
     gene            8766..9584
                     /gene="gspN"
                     /locus_tag="PLG98_07765"
     CDS             8766..9584
                     /gene="gspN"
                     /locus_tag="PLG98_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HOE33189.1"
                     /translation="MKILKNKIFWFTIYGIFITLVFLYLLFPSDIAQSRLEESIASSG
                     LILKTGSLKPSLPLGLKMKNVRLGSGSSPNIYFQGDSLDVQFHPLNFFRDKKNIRLSG
                     KAYGGTFSGNFKTTAAGFHPPEEGMLKFKNIALDRYTLIKALTGKQITGKASGNWTQA
                     AGSGGRENSSATISLFLNKGSYPLAEPFLGLQRIDFDRGEIQARLENRVLKFEKFQIF
                     SPQMDCSLNGDIMLVDEFKNSPVNLTGEIIISGRKVKMNITIEGTIANPSIRYM"
     gene            9590..10516
                     /locus_tag="PLG98_07770"
     CDS             9590..10516
                     /locus_tag="PLG98_07770"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOE33190.1"
                     /translation="MMFPEITAQIKERIGSFKRMPSVSSLDTGVLISVAVVLVITILS
                     YEATDLFYKVVRFPLTRHTAVIKNHHESFVRNNRQHQQLQDYDIITERNLFLTTLRTA
                     GGSESADGPFDADQKSMDFDLKGTIAISSSSGFIIVEERGSKKQKLYRLGDKIGARKL
                     AKITRNTAVLKNGEEEITVKIKETVEDNLLSPAPGSRRNPSPSRNPALSRLGIDGNAK
                     YLQSIMNQAVVRPFMSRGVRQGYIISNIKPSSLYEKAGLQNGDIIVGINNNPLHNAND
                     VMQVLNAMQSGSRLELTVKRRGRTETINYTLE"
     gene            10539..11405
                     /locus_tag="PLG98_07775"
     CDS             10539..11405
                     /locus_tag="PLG98_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE33191.1"
                     /translation="MYAAYFGLKENPFSLSPEPRYLFLSEQHRDALNCLIYGIKEKKG
                     FVLLSGDIGMGKTTVCRSLLASLDDSVETALIFNTAVSDIDLLETILEEYGVDIERES
                     RSKKNYIDRLNSFLLENFSAGKTAVLLIDEAQNLSHAVLEQIRMLSNLETETEKLIQI
                     ILIGQPEINNHLMLPALRQLNERITVRYALKPLSPPEVREYIHHRLKVAHGPGDIRFT
                     GAAYRLIFNFSEGIPRRINTICDRALLIAYTKSITKIDRKIVRLAVHDIGTTYFQKTM
                     SLGRKLLARLTV"
     gene            11439..12218
                     /locus_tag="PLG98_07780"
     CDS             11439..12218
                     /locus_tag="PLG98_07780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33192.1"
                     /translation="MSYINDALRKAQQDKKSPYAAYEPLLSPSGKKNTGRRRWLALLG
                     FLALFFCVAVAGLFVLMSGPETKKATAKAPLTIPTAPVIETPVIEAPVQPAVQDIKVE
                     NKVEPVLKTTEKKTQKTEESAALKIRTADSVKLYAEALRKHQEGNLEQAKDLYKKVIE
                     IDPKNIEALNNLGVVYMKKKTYKWAIIRLNDALKIKHDYPDAHYNLACIYAQNNDLSR
                     SLSYLKNAIGFNPQVRQWAEKDKDLEVLADLPEFKKLLEKN"
     gene            12226..14457
                     /gene="gspD"
                     /locus_tag="PLG98_07785"
     CDS             12226..14457
                     /gene="gspD"
                     /locus_tag="PLG98_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOE33193.1"
                     /translation="MKESHVLILKAGFLIWVMILLVIISGFAGSALAARVAGGGEEIE
                     TTAMPDAAPEPAIEEEVDPAPEPELMNEPSRQNAEMDAPVMSTDNKDEKRSYKPIKVN
                     PSQKYVTLDFDNVDVSVLVKFVSELTGKNFIIDDKVKGKVTIVSPKKIPVEDVYKVFL
                     SVLEINGLTVVPSGNMIKIIPSVQAREKGLETRTKQDPLLPEDRMVTQIIPLERANPD
                     EVKRILDPLIPKSSSVLSYPPAGMLIITDYLSNIKRIQEIATALDVEGAGDQISYIPL
                     QNASASEVVKSLNTIFTQGPVRGKAATILKVVADSRTNSIILLASGAETENVRKLIAF
                     MDKDVPRGESNIQVYRLQNSVAEDLAKVLTSIVKEGGAAAGATATATGAKTAPTTVVS
                     KNVQIVPDKATNTLVVMAEREDYKIIENIIKQLDIPRPMVYIEALIMEVNANKDFKLG
                     VEWRGITEHGSVDGNPTAAFAGSGGAGPSGPGSYNIMDGLLTTTGMGVFPSGFSMGLV
                     GAGIKIGGVTFPSIGAVVQAYKTDSEVSILSTPQIMTLDNEEAEINVGSNVPYVTRQD
                     QTNVGTGTNYTNYSSYEYRDVGVILKITPNINQENFIRLKIDQQVTKVQSGADEARPT
                     TLKRTAKTTVIVKDNETVVIGGLVGDSTDDSTYKFPLLGDIPLLGWLFKTKTSSREKT
                     NLYVFLTPHIVRTQADAAKIYQEKRETMGEVVEGVIKLNEKADAKAPQVRPPVENETI
                     SNP"
     gene            14478..16271
                     /gene="gspE"
                     /locus_tag="PLG98_07790"
     CDS             14478..16271
                     /gene="gspE"
                     /locus_tag="PLG98_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOE33194.1"
                     /translation="MSKIHTALKESAASAHGDASSGKSVFSSLDEKLIAAGVAPDTLS
                     EIRTAPANNGGGFFDQLIKKKVIPEIHLLKILSEHFGLSFWQQLPMESIDIDFTQRIP
                     IQYLKKHKIIPLITAENPVIALNDPSNFQPVDDLRQLLKNPNLSVVLSLQDAIMSAIN
                     MAYDMSRSSAKDYFEEMDQSSTDDLISKIDETADLLDEASDAPIIKLVNLILSGAIKD
                     RASDIHVEPYSANLKIRYRIDGILYDTLSLPRRIQSPLISRIKIMAKLNIAEKRLPQD
                     GRIEIKIADRLVDIRVSVIPTAFGERVVLRLLDKTANILMLSDLGMHDERIKLLNKLI
                     KSPYGIILVTGPTGSGKTTTLYAALSTINRPEINIITIEDPIEYQMDGVGQIQVNPKI
                     DLTFAAGLRSIVRQDPDVILIGEIRDRETAEIAIQSSLTGHLVFSTLHTNDAASAVTR
                     LIDMGIEPFLVTSSIVAIIAQRLVRVLCPHCKEVYEPDEESLANLGLSRSVLKNNTFY
                     RKKGCNLCMQTGFRGRSAIFEILTVDDEIKRLVLKTSDANQINELAIKRGMITLQQDG
                     IDKVLAGITTIEEVLRVTRTLSRTDDIVIEV"
     gene            16368..17186
                     /gene="dapF"
                     /locus_tag="PLG98_07795"
     CDS             16368..17186
                     /gene="dapF"
                     /locus_tag="PLG98_07795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOE33195.1"
                     /translation="MIEFYKMSGSGNDFIIIDNMDLSLDVGDLSEFARKVCQRKISVG
                     ADGLVVIEPSDIADFKWRFFNADGSMAEMCGNAARCVARFAYMKGIAGQTMSWETIAG
                     IISAVVNDDVVKAKLTDPSPVESNIEIETDGLKFILDSIDTGVPHAVVFVDDLDTCEV
                     YRQGRNIRFHEHFAPRGTNADFVSLINRNKIRVRTYERGVEDETLACGTGVVAAVLAA
                     AQRNLVESPVDVLVRSSESLRVYFENQNGLCREIYLEGRVKMVYQGMLLEEAYQ"
     gene            17365..>18221
                     /locus_tag="PLG98_07800"
     CDS             17365..>18221
                     /locus_tag="PLG98_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE33196.1"
                     /translation="MKTADKLAKIPPYLFMELRKKINKAKSEGIDVISLAIGDPVEAT
                     PDSIIDELCATSRDPLNHRYPTDEEKGMLAYRREIAKWYETRFGVTLNPENEILGLIG
                     SKEGCHHFIIARVNPDDIVLMTDPGYPAYRSSILLGGGVPYNVPILPQNNYLPVFEDI
                     PTDIAKKATAMFLNYPNNPTGACATLEFLDKLVEFARTYDIAVCYDNPYSEIVFSGQK
                     RISFLMAKGAKDVGIELNSLSKPFNMCGWRLGMACGNPELIAAISKVKENTDSGIFNP
                     IQFAGIKAL"
BASE COUNT         5153 a         4450 c         4412 g         4206 t
ORIGIN      
        1 accgttctgg gcgagcagat ttgcaaaatt atcaagaagc atctgggcat caatgtggaa
       61 tttggcggca acatcgctta cgatgaacac gttcacgacg ctatctgcca gcgcgtgtgc
      121 tttctggacc ggtatccgca cacgcgtgcc gccaatgatt tgcgtgtttt aaacaggaaa
      181 atgagcaatg ccttcggtga acaattgatg ctcaactatc tctgaaaaaa ttgaatcagg
      241 cggcacatgg aaaaagattt tgaccagatg aattattacg aaatcctgga catcggtacg
      301 aacgcgacat ctgcggaaat ccgcggcgcc tacaacgtcg cgttgcaaat gtatcagccc
      361 gattccctgg tcagctattc ttttttctcg aaaggagaac gcgataaaat tctctcgctg
      421 atcgacaagg cttattttac tctgatcaac gaagcccaga gagccaaata cgacaacgaa
      481 atgaatctgc gggaaacgtc cgtttccgca gaccaacaca aagccccccc ccaaccggcc
      541 aacatcttcg aatttaaccg ccgggatatc gcttccctga cgcggaaaaa caacaaatcg
      601 gcattaaaga caaaaatttc ccaaagccag tccatcaggg aaattctttc ccggcagaaa
      661 atcaccggcg ccgacctgaa ggccatcagg gaagaactcg ggctggcaat tgaaaccatc
      721 catcaggaaa caaaaatccg tcttgattac cttcatgaca ttgaagaaga caaaacggaa
      781 aaactgccgg cacccgtctt tttaaaagga tttgtgaaag cctatttgcg aagcctgtgc
      841 attgagaatg cagatgatat cagcacggcc tacatgaaca cgttacccgg taaaaattaa
      901 cttgacagcg ctaatcttta gtggtcatta tttgttaaaa tcatagcatg aaaaattcct
      961 gaagcgtcgg caatgaaagt cctcattcat ctgaaaattt accgtcccca aaggtgcttt
     1021 ccggtttttt atgacgattc aaaaggagca taaatgaaaa aatcactatt ctatttactg
     1081 ttaatcagta tgctgatctt ttttgtttcc gcctgctcgg acagcgattc agattttgac
     1141 gatacagccg acgcggaata tctgacggtt caaatgagag ctgatgccgg caacggagaa
     1201 gtctcgcttg actggcagat gcttcccaga gctgaaacat acaacattta ttatatggaa
     1261 gatgacgaag acgataccaa tcaacccacc tcgcaaaaaa tgaaatctga aggcacaaaa
     1321 atagatggag aaacaaacca catcatcagc gcgccgtatg tagttaccgg tctggaaaac
     1381 gggaaaacgt actggtttgc cctgtccgcc aaaaatgccg agcaggcgga aagcgacctt
     1441 agccaggcta tttatgcgat tccccgggct aatccgccat tgcctgctcc taaaaacgtc
     1501 cgcgccaacg caggtgataa agaggccact gtgacgtggg atgcggtaac cggcgcggcc
     1561 ggttacattg tgtattacta cacgtctttt gcaaactatc aggaaagccc taaaatcacg
     1621 agcacctcgt atacattcac aggacttacc aacaaacaaa cctatctttt tttcgttgcc
     1681 gcagtcgaca gtaacgacaa caacgaaagc ggcgacagca gcgcttcgtt tgtctacacg
     1741 acaacgccaa gcgaagatcc gcctccatcc gcgccccgta atcttcgggt catcaatcgc
     1801 ggtgaaggat tcgttgatct ggcttgggat gctccattat tccacacagg agcattaacc
     1861 tataatgtct attttttcac cgccggcaac attacaaaat ccacagccca aaaagggata
     1921 gaggccctgg cggatacgtc tacccgggcc gcgaatctcg ataaagaaaa aacgcattat
     1981 tttgtcgtca cagccgtaga caacaatgga gaaagcgcgg aatcaggaga agtttccgca
     2041 atccctgatt tataaaaagg aattattaag caaatcaatt ccggataaaa agggcttttt
     2101 aggccctttt ttattatacg tgacagaaag tcgggacaat gcgaaacaaa agacacggga
     2161 ttgaatcgta ataaaattta tgttaaacgc gcccataggc attatggcag gcggagcaaa
     2221 aattaatgag tgtttttgaa tacatcgcgc tggatgaaaa agggagacaa ctgaagggat
     2281 ttatcgatgc ttcaggtatc tccgcagcgc gtcaaaagtt acgggaagaa aatattttcc
     2341 ccgtggaaat caatcagacg gaaacaaaaa aagaatccgc tctggccggg gctctgaaga
     2401 taaatctctg ggaaagggtt tcggcaaacc aggtggctgt ctttacccgc cagctttcca
     2461 cattactggg agcaggcatg cctctggtcc cgtcgctttc cattctgatg cagcaggaaa
     2521 aaaatccgct tctgaaaaag tccctggcgc aaatccgtga acaggtcaat gaaggcaaaa
     2581 gcctgacgga agccatggcg gaatttcccc gcatctttcc ttctttttat ctgaacatgg
     2641 tgagagccgg cgaggcttcg ggaaccatca acctggtttt agagcggctg gccgacttca
     2701 gtgaaaatca gcaggccctg atgaataaaa tccgttccgc cctagcctac cccatcatca
     2761 tgtttatcat gggcagcgcg gttatctttc ttttgatgac gtttgttgtc cctaagataa
     2821 ccggaatttt tacggatatg aatcagacat tgccgttggt caccatcgtt ctgattaaag
     2881 tcagcaattt tctgaaggcg ttctggtgga tcatcctgat ccttttgttc ggtggacttg
     2941 ccgctattag atatatgaca acgggcaccg attccggcag acgcgcctgg gataacatcc
     3001 ggctgaaaat tccggtcatg ggacaaatca accgcaaaat tgctattgcc cggttttgcc
     3061 gaacgctggc cacacttttg caaagcggcg tgccgcttct gacagcgatg gaaatcgtcc
     3121 ggaacattgt caacaatgtt atcatcggcg atgccattca tcaggcggcc agagatcttg
     3181 aagaaggcaa agggctgtcc ggtccattga ccaaaagcgg tttgtttcca ccgctggtga
     3241 cggaaatgat tgctgtcgga gaacaaagcg gtacactgga gaccatgctt aatcgtattg
     3301 ccgcggctta tgaaaacgaa tcacaggcca gtatcacggt catgacgtcg ctgctggaac
     3361 ccatcatgat tctgtttatg gggctggtgg tgggttttat cgttgtctca attttattac
     3421 cgattttcga aatgaatcag ctggtcagat aatttagaca tccgtaaaaa agggggtttt
     3481 atgaaaattt tcagaagaaa aaaaagcaga caggcggggt tcaccctgat tgaactgatg
     3541 gttgtcatcg tgatcctggg tgttcttgcc ggtatgatta ttccccgcat catgggacgt
     3601 cctgacgaag ccagacaggc caaggcaaga atccagatgg aagccatgga atcggcgctg
     3661 aaactctaca agctggacaa cggcagttat ccttcgacgg aacaggggct aaaggctctg
     3721 gtggaggccc cgacaacggg agatctcccg aagaactggc gtcagggagg atatctggaa
     3781 aaagggaaag tgcccaaaga cccatgggga aacgattttg tttatttaag tcccggcagt
     3841 cacggcgatt ttgatttatc ctgtctgggc aaggacggtg aagccggagg agaaggtgtc
     3901 gatcaggaca tcaacaactg ggaagtggag taagcaggga ttcacgcttg ttgaattact
     3961 gcttgttatc acgctgatcg gcatagttct ggtgctggcc gttccgtcca cacgggacgt
     4021 tctcaccggc gacatgctta agaaaacgtc gcgccagttg atcggcctgg aaagaaaact
     4081 cagaacggaa gccgttcgcg atcagctgga ctatattctc catctggatt tatccgactc
     4141 cgtctactgg attacggctt ccgacatgac agagatcaaa caggctgaaa tcaaaaaaca
     4201 ggctctgcat ttgcctgagg atgtccgcat tatcgatgtc gtggacgaag acaaccggaa
     4261 gattaccgcc ggcgaagtaa aaatcgtctt caaaaaggat aacatctgtt ccccgagagt
     4321 gattcatctg gcttacaagg aagataaaat gacgatcgtc atgaatccct ttatgggtgt
     4381 caccgatatt tacgaccagt acgtggaaat tcctttcaac acctccgaag ggcaaaatcg
     4441 ccggtgagga ccaggctatg acgcgaatga cagaaaaagg atttactctg atggaagtca
     4501 tggtcgccat ggccattctg gcgattgcgc tggtttccat ctttcagctt cagtcgcaaa
     4561 gcatttccat ggcgacggat tcgcgtttca tgacgaccgc cgcacttctg gcccagagca
     4621 aaatggtcga ggttgaaaca cagagcccct tatccaataa aaccgaaaac ggcgatttcg
     4681 gccccgacta tccgcaatat gcctggcagt taatcattgg cgatacccag ttgcctcaat
     4741 tcaaaaaaat cgaggtcacc gtcaccaaca aactatttct tcgccgcgga acgtacacac
     4801 tgatcctcta taaaaggccg gatatgtaac tatgagacat tgctcttcca gaccccattt
     4861 aagcggcggc ggattcacac tcgtggaaat tctcatcgct atcttcattc tctctctggt
     4921 gctggccacc gtttatgttt cctataccgg caccctaaaa acatcgcacc agttggaaaa
     4981 agaaggcgaa atctacaaaa tggcgcgcca gagcctggag cggctggcga aggatttaac
     5041 gtctctgcag atgtccgagg gatcgttcta cctgaacgcc gaaaagaaaa aaacaggacg
     5101 gcatcaattt cacaacattt ttttctggtc ggcagcccat ttgactttcg gagaactgga
     5161 aggtgagggg cgaccggcaa cgatcggata ttacgtgacg gaaaatcctg atggaaaaag
     5221 cttctcgctc tggagatccg acgctaccgg aatgaaaccg gatgagacaa aaaaaacaga
     5281 agacggattc atcatctgta aaaacattga aacatttcat ctgacgttct acgattcggc
     5341 cgggggagaa aatgacacat gggagacgtc cgccggtgga ggcatgcatc agggcaaagt
     5401 acccgaagcg gtgaagatag agcttgcgct ggtcaacgat gatgatgcgc agaagccata
     5461 caaattcatg accagagtat tcttaccggt gaacaaataa aatgatgaac atattcgcca
     5521 gacatcagga tgatcaaaaa aagaatgacc cccaaaagtc atttcttaaa ttttctatcc
     5581 tcaaaggttt catggccttc cgaaaacgcc gggatggttc aacttaccaa tttccgttca
     5641 ctgcgttccg gaaattgttg tttcacaacc cactgctgtc gcgggatggt tcaacttacc
     5701 aatttccgtt cactgcgttc cggaaattgt tgtttcacaa ccctcgaggg gttgccctga
     5761 tcacggtcat tttgatcatt gtcattctga ttgccgtggt gatcgagttg aacagatcgt
     5821 cccgcgccga tatttacgat gcagccaact taagcgacgg tataaaatta agctatatcg
     5881 ccaagtcggg attttacgga gccgccgccc tattgaacaa ttccccgggc gcttacacct
     5941 ctctgcgcga cgactgggcg aaaatggaaa cggtatctct gcagtccaat actctgtttc
     6001 cggaagggta ttttatcgcc agaatcgaag atgaagcggg aaaaatccct ttgaacaagc
     6061 tggttgacgg cgaagaatac aatgaagaaa tcagaaccat gctcgaccgg ttactggcac
     6121 agccggaatt cggcctgaag gacagacaaa tcgatgaaat catcgatgcc atcaaagact
     6181 ggatcgacac agatgagaac acgaccggtt acggagcgga aagcaattac tacgaatctc
     6241 ttgattctcc ctatcaggca aaaaacgctc cgtttgattg cattgaagaa ttgcttatgg
     6301 taaagggaat aaccaaagaa atcttttatg gaacgaaaga caaaccatca ttggcaaaat
     6361 atgttaccgc agacagcgaa ggattgatta acatcaatac ggcgcccaaa atagttttac
     6421 gctctctcag cgatgacatt agttccgagc tggccgacag gctcgatgaa taccgcatgg
     6481 agaagagcca tgatgtttcc ggaaatcaat ggtacaaaca ggtgcccggt atggaaaaca
     6541 taaccattaa tcaaaatctg attacggtca aaagcactta ttttaaaatc gtatccacgg
     6601 gcaaaatcag caatatgtca agaaccgtga ccggcatcgt cgccacgggg gaccaggctc
     6661 agattgcgat catcacgtgg aggcaggatt aatgccgaat gttctgggac tggaaatcgg
     6721 ccggagcaca attaaagccg ttctgatggc ccgaaaaggc ttgggtggcg gtcgtattct
     6781 tgacgcgcgc attctggacg tgcgcgacag cgaaggaatt ggaccggcac tgcagaaatt
     6841 ggcggaagat aaaaattttg caggtatccc ctgctttgtg tctgtgcctt cccaggacgt
     6901 cctcttccgt catgttcgcc tgcccttccg cgatgataac cgaatccgca aaacgctgct
     6961 cttcgaactc gaaccgctca ttcctctgcc cattgaaaac gtcattgcag actacatcgc
     7021 ccttccccgc gaaggactgc tcgtcggcgc catgcccaaa gaaaccatcc gcgaaattat
     7081 tgaacaggtg gaaaaaaatc tcggcgacgt ctcggttatt gacacctctt cttcggcgct
     7141 ggccgtacag ataccggatc ataaaaaaac ttccgtcagc atcattctcg atatcggacg
     7201 caatgcgacc acagccgcat tttgcgaaaa tgaatctgtg attcatatcc gttctctggc
     7261 cttcagcggc gaacaaatca cgctggcgat ggcaaaagat ttttccgtgg acaggaatcg
     7321 cgcggaacag ctgaaaatca gcggcgatta tcccgaggct atggaaaagg ccggggagga
     7381 atgccggggg ctttgttcgg aattgaaaaa caccgtcgaa ttcatgaaga taaacggcgt
     7441 tctgcacaat gatcctgcac aaatcctgct gacgggcggc ggggctctgt tcggtccgct
     7501 gcgcaaagag atcgagaaaa actttacctg cccggtggaa cttctcaacc tgatcagtgc
     7561 cagacaactg gaagttgaaa aaaatatcat gatgcggatg aatccgtctt tattgaatgc
     7621 ggccgcggcc aacgcgctga gaagttcggc cggcaaaaaa tcattcaatt tccgccaggg
     7681 tgaatttacc gcccggccgt cgcatctgaa tttcaaaaat cacctgcgct gggcgggcat
     7741 ccttgcggcc gtgatcctgc tgctggccgt ggtgaatcaa attctcgatt acagcctgaa
     7801 aatgcgacaa attggcggca tcaagaagga cattgccagg attttccaaa agagttttcc
     7861 ggacgctccg tccatggtgg atccggtgca gcaattgaaa acaaaaatcg aagaagataa
     7921 aaaaacattc ggtctcggcg aaggaatgcc ggaagcaaca acagccgatt ttttaaagga
     7981 gctctcagcg ttgatatcgc cttccctgga aatcgtcttc actgcactga cgtatgaaaa
     8041 cagggtcatc tcggtaaaag gagaggcgaa aacggtcgat gatgtgacgt tgattaaaga
     8101 cgcgctggcc aaatcaaaat ctttcaaaga ggtgaccatg gggccgacga gtctgacccg
     8161 tgacggcggc aaagttgatt tcagtttaag gattgaagtc caatgataca ggaactgtgg
     8221 aacaaactgg atgccacaca gcgcaaactt gttgcgggcg cggccgtgtt cgtctgtatc
     8281 gcgctgattc tggaactggc cgttttcccc ttctgggaag ctaaaaagaa actggacaga
     8341 tccatcagca gcaaccagaa aaaactggcc gaggtcaaag aactttccgc cgagttcaat
     8401 cggttggaat cgaaagtgtc cgccatgcga agagtcgctt cgatgcgcgc ggcaaatttt
     8461 acgctgtttt cccatctgga aaagaaagcg acgcaggccg gcgtcagaaa cagaatcaaa
     8521 aacatgaact cctccagagg cattcaatcc gcctcctttg aggagtcttt gatcgatatg
     8581 aagctggaga aggtcaccat caaacagctc gttgatttcc tttattacgc ggaatcgccg
     8641 gcggatttag tgagagtcaa aaagatccat attgtaaaaa tgaaggacgc tccggaatat
     8701 ttaagtgtgc aattgcagat atcttccatt cagccgtcga atcaaattcc ggaaagcggg
     8761 caaccatgaa aatactgaaa aacaagatat tctggtttac gatttacggg atattcatca
     8821 cgctggtctt tctgtatctg ctctttccct cggatatcgc ccaaagcagg ctggaagaat
     8881 ccattgcctc ctccggtttg attttgaaaa ccggctcatt aaaaccgtcc ctgcccttgg
     8941 gtctgaaaat gaaaaatgtc cgtctgggtt cagggtcctc cccaaatatt tattttcagg
     9001 gagattccct cgacgtacag tttcacccgt tgaatttttt ccgggataaa aaaaatatcc
     9061 gcctgagcgg aaaagcatac ggcggaacct tttcgggaaa tttcaagacc acggcggccg
     9121 gttttcatcc gccagaggaa gggatgctga aattcaagaa catcgcgctg gaccggtaca
     9181 cgctcatcaa agcgttaacc ggtaaacaga taacaggcaa ggcgagcggt aactggacgc
     9241 aggcggcggg atcaggggga cgtgaaaact cttccgcaac catttcgctt ttcctcaaca
     9301 agggatctta tccgctggcg gaacccttcc tgggactgca acgaatcgat tttgaccgtg
     9361 gcgagattca ggccaggctg gaaaaccgcg tcctgaaatt cgaaaagttc cagatcttca
     9421 gcccgcaaat ggactgttct ttaaacggcg acatcatgct tgttgacgaa ttcaaaaaca
     9481 gcccggtaaa tctaacgggc gaaataatca tatcgggtag aaaggtaaaa atgaatatca
     9541 ccattgaagg taccattgcc aatccttcta ttcgctatat gtaggtaaga tgatgtttcc
     9601 tgaaattaca gcgcagatca aagaacgtat cgggtctttc aaaagaatgc cctccgtttc
     9661 atctttggat accggtgtct tgatctctgt ggccgttgta ctggtcatca ccattctgtc
     9721 ttacgaggcg accgacctgt tttataaagt ggtccgtttc ccgctgacca gacacactgc
     9781 cgttatcaaa aaccatcatg aatcattcgt caggaacaac cggcagcacc agcagcttca
     9841 ggattacgat atcatcaccg aacgcaatct gtttttgacg acgttgagaa cagcaggcgg
     9901 aagcgaatct gcggacgggc ctttcgatgc cgaccagaaa agcatggatt tcgatctgaa
     9961 aggaaccata gccattagtt cctcttccgg ttttattatc gttgaggaac ggggaagcaa
    10021 aaaacagaag ttataccgcc tgggggacaa gatcggcgcc cgcaagctgg ctaaaataac
    10081 aagaaacacc gccgttctga aaaacggcga ggaagaaata acagtaaaaa tcaaagagac
    10141 ggtggaagat aatttgcttt cccctgcccc cggctccagg aggaatccct cgccttcaag
    10201 aaaccctgct ctttccagac tgggaattga tggaaacgca aaatacctgc aatccattat
    10261 gaatcaagcg gttgttcgtc ccttcatgag caggggcgtg cgtcagggat acatcatttc
    10321 caatatcaag ccgagtagcc tttacgaaaa agccgggctt cagaacggcg acatcatcgt
    10381 cggaatcaac aataatccgt tgcacaacgc caacgatgtc atgcaagttt taaatgcgat
    10441 gcagtccgga agccgtttgg aactcaccgt caagcgaaga ggacggacgg aaacgattaa
    10501 ttatacgttg gaatgattgc ctcgggagaa aaatatccat gtatgccgcg tattttgggt
    10561 tgaaagaaaa tcccttcagc ttgtcgccgg aaccgcgcta ccttttttta agcgaacagc
    10621 accgcgacgc cttaaactgc ttgatttacg gaatcaaaga aaaaaagggg ttcgttctgc
    10681 tttcgggcga tatcggcatg ggcaaaacca cggtctgccg cagtctgctg gcctcactgg
    10741 atgattcggt ggaaacagcg ctcattttca ataccgccgt ttccgatatc gatttactgg
    10801 aaaccattct cgaagaatac ggcgtcgaca tcgaacgcga atcccgaagc aaaaaaaatt
    10861 atatcgaccg cctgaacagt tttctgctgg aaaatttctc cgccggcaaa acagccgttc
    10921 tgcttatcga tgaggcgcaa aatctatcgc atgcggtgct ggaacagatc cgcatgcttt
    10981 ccaatctgga aaccgaaacg gaaaaactga ttcagattat tctgatcgga caaccggaaa
    11041 tcaataacca tttgatgctg cccgctctgc ggcagttgaa tgaaagaatc acggtccggt
    11101 atgctttgaa accgctatca cctccggaag tgcgcgaata cattcatcat cgtctgaaag
    11161 ttgctcacgg cccgggggac attcgtttca ccggcgctgc ctacaggctc attttcaatt
    11221 tttcggaagg catcccgagg cggattaaca cgatttgcga ccgggcgctc ctgatcgcct
    11281 ataccaaaag cataacgaaa atagatcgca aaatcgtcag gctggcagtt cacgacatcg
    11341 gcacaactta ttttcaaaaa accatgagtc tgggcagaaa attactggcg cgtctgaccg
    11401 tctgaccgaa gctggaaagt cttgaccaaa gggatattat gagctacata aacgacgcat
    11461 tacgcaaagc acagcaggac aaaaaatcac cctatgcggc atatgaaccg cttttgtcgc
    11521 cttccggtaa aaaaaatacc gggcggaggc gatggctggc cctgctgggg tttttggcat
    11581 tgtttttttg tgtggcggtg gcaggacttt ttgttctgat gtccggccct gaaacaaaaa
    11641 aagccacggc caaagcgccg ctgacgattc cgacggcacc ggtgattgaa acgccggtga
    11701 ttgaggcacc ggttcaaccg gctgtgcaag acataaaggt tgaaaataaa gtcgagccgg
    11761 tattaaaaac gacagaaaaa aagactcaaa aaaccgaaga aagcgctgca ttgaaaatac
    11821 ggacagccga ttcagtaaaa ctttacgccg aagcgctgcg gaaacatcag gaaggcaacc
    11881 tggagcaggc aaaggattta tataagaaag ttattgagat tgacccgaag aatatagaag
    11941 cgctgaataa tttgggtgtc gtttatatga agaagaaaac ttacaaatgg gcaattattc
    12001 gactcaacga cgcgctgaaa ataaaacatg attaccccga tgcgcattac aatctggcct
    12061 gtatctacgc gcaaaacaac gacctgagcc gaagtctttc ctatttgaaa aatgctatcg
    12121 gctttaaccc tcaggtcaga cagtgggcag aaaaagacaa agatcttgag gttctggccg
    12181 atttgccgga atttaagaaa ctattggaga aaaattaacg tcaagatgaa agaatcacat
    12241 gttcttattt tgaaagccgg ttttttaata tgggtcatga tcctgttggt gattataagc
    12301 ggttttgccg gttcagcgct ggccgcgcgt gtcgccggcg gcggtgaaga gatcgagact
    12361 acggctatgc cggatgccgc tccggaaccg gcgatagaag aagaggtgga cccggcgccg
    12421 gaacccgagc tgatgaatga accttcccgt cagaatgccg agatggacgc accggtcatg
    12481 tctacggaca acaaagatga aaaacggtct tacaagccca ttaaagtcaa tccgtcgcag
    12541 aaatatgtca cgctggattt cgacaatgtg gatgtcagcg tactggtaaa attcgtcagt
    12601 gaattgaccg gtaaaaattt catcattgac gacaaagtca agggtaaggt caccatcgtt
    12661 tcccccaaaa agattcccgt ggaagatgtc tataaagttt ttctgtccgt tctggaaatt
    12721 aacggtttga ccgtggtgcc ctcgggcaat atgatcaaaa tcattccctc tgtgcaggca
    12781 cgggagaaag gtctggaaac caggacaaaa caggatcccc tgttgccgga ggatcgcatg
    12841 gttactcaga tcattcccct ggagcgggcc aatcccgatg aagtgaaaag aatcctggat
    12901 ccgcttattc cgaaatccag ttctgttctt tcctatccgc cggcaggcat gctgatcatt
    12961 accgattacc tttccaacat caaacggatt caggaaatcg caaccgcgct ggatgtcgaa
    13021 ggcgccggcg atcagatatc ctatatcccg ctgcagaatg cttccgcttc ggaagtggtc
    13081 aaatcgttga ataccatctt cacacagggt cccgtccgcg gaaaggccgc gacaatctta
    13141 aaagtcgtag cggattcgcg caccaattca atcattctgc tggccagcgg cgccgaaaca
    13201 gaaaatgtgc gcaagctgat cgcatttatg gacaaggatg tgccgcgcgg agaatccaat
    13261 attcaggttt atcgtttaca aaacagtgtc gccgaagatc tggcaaaagt gctcaccagc
    13321 attgtcaagg aaggcggggc agcagccggc gccacagcaa cggcaaccgg agcgaaaaca
    13381 gcaccgacga ctgtcgtttc caaaaatgtt caaatcgttc ccgataaagc gaccaacacg
    13441 ctggtcgtga tggcggaacg ggaagactac aagattattg aaaacatcat caagcagctc
    13501 gatattccgc gtccgatggt ctatattgaa gctttaatta tggaagtcaa cgccaacaag
    13561 gatttcaaac tcggcgtcga atggcggggc atcaccgaac atggctccgt tgacggaaat
    13621 cccacggcgg cttttgccgg ttcgggaggc gccggccctt ccggtcccgg ttcctacaat
    13681 atcatggacg gtttgctgac aacgaccgga atgggcgtct ttcccagcgg tttttccatg
    13741 ggactggtcg gggcaggcat taagatcggc ggcgtcacct tccccagcat cggtgcggtc
    13801 gttcaggcct ataaaaccga ttcggaagtt tccatcttat ccaccccgca gatcatgaca
    13861 ctggacaatg aagaggcgga aatcaatgtg gggtcgaacg ttccctatgt gacaagacaa
    13921 gatcaaacaa atgtgggcac cggcacaaat tatacgaact acagcagcta tgaatacagg
    13981 gatgtcggcg tcattttaaa aatcacgccg aatatcaatc aggaaaactt tatccggctg
    14041 aagatcgatc agcaggtgac caaggtgcag tcgggcgccg atgaagcaag accgaccacg
    14101 ctgaagagaa cggcaaagac aacggtgatc gtcaaagaca atgaaacggt cgtcatcggc
    14161 ggtctggtcg gcgacagtac ggacgacagc acgtacaaat tccccctgct gggcgacatt
    14221 cctcttctgg gctggctttt caaaacgaaa acatcatcac gggaaaagac caacctttac
    14281 gtatttctga caccgcacat cgtccgtacg caggccgatg ccgcaaaaat ttatcaggaa
    14341 aaaagggaga ccatgggaga agtggtcgag ggcgtcatca aactgaatga aaaagccgat
    14401 gcgaaggctc cccaggtgcg tcctcccgtt gaaaacgaaa ccatttccaa tccgtaagcc
    14461 tgagggtccg attcgatatg tccaaaatcc atacagctct gaaagaatcg gcggcgtctg
    14521 ctcatggtga tgcatcgtcc ggcaaatccg ttttttcgtc tctggatgaa aagctcatcg
    14581 ccgcgggagt ggcaccggac accctcagtg aaatacgaac cgcaccggca aacaacggcg
    14641 gaggattttt cgatcaactg atcaaaaaga aagtgattcc tgaaatccat cttttaaaaa
    14701 tcctttccga acatttcggg ctgtcattct ggcagcaact gcccatggaa agcattgata
    14761 tcgactttac ccaacgcatc cccattcaat atttaaaaaa acataaaatc attccattga
    14821 tcaccgccga aaacccggtg atcgcattaa acgatccttc gaatttccag cctgtcgatg
    14881 acttgagaca gctcctgaag aatccaaatc tgtcggttgt tctgtctttg caggacgcaa
    14941 ttatgtcggc cattaatatg gcgtatgaca tgagccgctc atcggccaag gattattttg
    15001 aagaaatgga ccagtcctcc accgacgatc tcatttccaa aatcgatgaa accgccgatc
    15061 tgctggatga agccagcgat gccccgatca tcaaactggt caacctcatt ctttccggcg
    15121 cgatcaagga ccgcgccagc gatattcatg tcgagcccta ttcggcaaac ctgaaaatcc
    15181 gctaccgcat tgacggcatt ttatatgata cactcagcct gccgaggcgc atccagtcgc
    15241 cgctgatatc acgtatcaaa atcatggcca aactcaatat cgcggaaaaa cgtctccccc
    15301 aggacggacg catcgaaata aaaatagccg atcgcctggt ggatatccgc gtgtcggtca
    15361 tccccaccgc cttcggcgaa agagtcgttc tgcgattgct ggacaaaacg gccaacattt
    15421 taatgttatc cgatctgggc atgcatgatg aacggatcaa actcctcaat aagctgatta
    15481 aatcgcctta cggaatcatt ctggtgaccg gccccaccgg cagcggtaaa acaacgacgt
    15541 tgtacgcagc gctttccacc attaaccgtc cggagatcaa tatcatcact atcgaggacc
    15601 ctatcgaata tcaaatggac ggcgtgggac aaatccaggt caatcccaaa atcgatctga
    15661 cgtttgccgc cggcctgcgt tctatcgtcc gtcaggatcc tgatgtcatt ctcatcgggg
    15721 aaatccgtga ccgggaaacc gctgaaatcg ccattcagtc ctcgctgacc ggacatctgg
    15781 tcttctccac gctgcatacc aacgatgccg ccagtgccgt tacccgtctg atcgatatgg
    15841 gcattgaacc ttttctggtc acatcgtcta ttgtcgcgat catcgcccag cgactcgttc
    15901 gcgttctctg cccccattgt aaagaggttt atgagccgga tgaagaatcg ctggccaatc
    15961 tcggtttgag ccgatccgtc ctgaaaaaca acacttttta ccgcaagaaa ggctgcaatc
    16021 tctgcatgca gacgggtttc cggggacgtt cagccatttt cgaaattctg accgttgatg
    16081 atgagattaa aaggctcgta ctgaaaacat ccgacgccaa tcagatcaat gaactggcca
    16141 tcaagcgggg catgattaca ttgcagcagg atggtatcga caaggttctc gcaggcatca
    16201 cgacaatcga agaagtgctg agagttaccc gcactctcag tcgtaccgat gacatcgtca
    16261 tcgaagttta atgaggctct ctgaactata gtgaagagtt agccgaatta tccccgaagc
    16321 gaagcggagt gggatagtga gtctcgctac tggaaacgga ataaaacatg atcgaattct
    16381 ataaaatgag cggcagcggc aatgatttca tcatcattga caacatggat ttgtccctgg
    16441 atgtcggtga tttatccgag ttcgcccgca aagtctgtca gcgcaaaatt tccgtgggcg
    16501 ccgacggact ggtggtgatt gagccgtccg acatcgccga tttcaaatgg cgttttttca
    16561 atgctgacgg ttccatggcg gaaatgtgcg gcaatgccgc ccgatgcgtg gcgcgtttcg
    16621 cttatatgaa aggaatcgca ggacaaacaa tgtcttggga aaccattgcc ggtatcatca
    16681 gcgcggttgt aaatgacgac gtggtcaagg caaaacttac cgatccctcg ccggttgaat
    16741 ccaatatcga aatcgaaacg gacgggctga aattcatctt ggacagtatt gataccgggg
    16801 ttccgcacgc ggtagttttt gtcgatgatc tcgatacctg cgaagtatat cgtcagggac
    16861 gcaacatccg tttccatgaa cactttgccc cccggggaac caatgccgat tttgtctcac
    16921 tcattaacag aaataaaata agggtccgga cctacgaacg cggcgtcgaa gatgaaacgc
    16981 tggcttgcgg aacgggtgtg gtggcggcag ttctggcggc agcgcaaaga aatcttgttg
    17041 aatcgcccgt ggacgtgctg gtacgaagca gtgaaagcct gcgcgtttat tttgaaaatc
    17101 aaaacggcct gtgtcgtgaa atttaccttg aaggccgtgt caaaatggtc tatcagggaa
    17161 tgcttctgga agaagcctat caataaactt aataaaatat atctgtcatt ccggtgcgga
    17221 ccggaatcca gtattattta attaaatatc aatttctgga catcggcctt cgtaacctaa
    17281 ataacctgaa ggtcactata gggtcatcta cgaccggtgt gacaattaga ttctaatcat
    17341 aattaatttt ggaggatatc gattttgaaa actgcggaca aactagcgaa aataccgcct
    17401 tacctgttca tggaattgag aaaaaaaata aacaaggcca agtctgaagg cattgatgtt
    17461 atttcactgg ccatcggcga tccggtggaa gccacgccgg attctattat tgatgagctt
    17521 tgcgccacgt cgcgcgatcc tctgaatcac cgttatccga ccgatgaaga aaagggtatg
    17581 ctggcatacc gccgcgagat tgccaaatgg tacgaaacac gcttcggcgt tacgctgaat
    17641 cccgaaaacg aaattctggg attgatcggt tcgaaggaag gctgtcatca ttttatcatc
    17701 gcgcgcgtca atcccgatga catcgttttg atgaccgacc ccggttatcc cgcttatcgt
    17761 tccagcatcc tgctgggcgg cggcgttccg tataatgtac ccatcctgcc gcagaacaat
    17821 tatctgccgg tattcgaaga cattccgacg gacatcgcta aaaaagccac cgccatgttt
    17881 ttgaattatc ccaacaatcc gaccggcgcc tgcgcgaccc tggaattttt agacaagctt
    17941 gttgaatttg ccagaaccta cgacatcgct gtttgttacg acaatcccta cagcgaaata
    18001 gtttttagcg gacaaaaacg catcagtttt ctgatggcca aaggcgcgaa agacgtcggc
    18061 atcgaattga actccttatc caagcccttc aatatgtgcg gatggcggct gggcatggcc
    18121 tgcggcaatc ccgaattaat cgccgccatc agcaaggtta aggaaaacac ggactctgga
    18181 atctttaatc cgattcaatt cgccggcatc aaagccctgc a
//
LOCUS       DAOSME010000046        17871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3772_length_17871_cov_23.507016, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000046 DAOSME010000000
VERSION     DAOSME010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3772_length_17871_cov_23.507016"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLG98_07805"
     CDS             complement(<1..463)
                     /locus_tag="PLG98_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33197.1"
                     /translation="MMTQAKKDPFVGLYKKMLELSPSVCRKEFTFGGYSYGDVLALAA
                     GLKKILDRRSGKKNICLCSANKAVTAACVMASLTGACNVILPYSFSTHAMKEMSEATG
                     FNFAIADHPEEMPEGVEIITPVPAAPETLRSESLIHPDETFLRLFTGGSTGK"
     gene            600..1028
                     /locus_tag="PLG98_07810"
     CDS             600..1028
                     /locus_tag="PLG98_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33198.1"
                     /translation="MLDIDIDSLIPHRKKIKIINHVIDIQENSAVSAAMVGPDWPRSD
                     GKTVDALVLIEGIAQTAAIVEGYKRKQRGVGAVKGWLVGIKNAAFYEEKIPLNTHLVI
                     LLENKDAFDNYGVVEGTVKTGEKILATAVLQAVRLNDNDQ"
     gene            1052..1780
                     /gene="fabG"
                     /locus_tag="PLG98_07815"
     CDS             1052..1780
                     /gene="fabG"
                     /locus_tag="PLG98_07815"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOE33199.1"
                     /translation="MNDKKTAIITGASRGIGRATAVQLARAGYFVIINFKTNETAAQE
                     TLNMVKSEGGDGTIIPFDVADSEQTGNAVKAITEQYKNIQVLINNAGITADGLFMLLG
                     EEEWGSVINTSLNGFYHMTKPIVREMIRKKRGSIVSVSSLSALMPNRGQANYAAAKAG
                     IIAASRSLASEVARFGVRVNVVAPGLIETDMIKDAPVDQIKQIIPMARLGKPQEVASV
                     IRFLCSDDASYVTGQVIGINGGIC"
     assembly_gap    2015..2114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2115..2570
                     /locus_tag="PLG98_07820"
     CDS             <2115..2570
                     /locus_tag="PLG98_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOE33200.1"
                     /translation="PLVSQGYFYFAMPDSLRWEYIKPLKSVVLSHNQQARRYMHSDGK
                     WVEDKTNGVQAMRIVLNEIAGWMNGRFDQNPSFKATVIGKENTCIILTPVGKKMKGMI
                     ERIEINLAPQARVVQSVKILEGPDNITQINFSNVKMNHAIDPTIFKDVR"
     gene            2567..3235
                     /locus_tag="PLG98_07825"
     CDS             2567..3235
                     /locus_tag="PLG98_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33201.1"
                     /translation="MRRHLLWIVIFFLSSCQTLPIINAPSSPRKEKSFVCPDPFLKRN
                     YRLVHAIEARLDGRVQSQIIGITLALPDTRSVSCAIITVEGMSLFEAEANPHGINVKR
                     ALPPFDSKDFANNMMEDIKLIFLAPEGVLQNRGYLADGSKACRYRQENGGWIDVIENE
                     SGETQIKRYSSSEVLKRDVRFSGTHKQLYQRIGLQADAKFDYFLLMNLIEDQPIKKQA
                     IKRN"
     gene            3452..7795
                     /locus_tag="PLG98_07830"
     CDS             3452..7795
                     /locus_tag="PLG98_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOE33202.1"
                     /translation="MKSHHLHHKGKSFSGKQGETVMVAGCDLGKSTAKFVMLNVCDSD
                     TPILEDFRCIVHHGNPLNAFREWYREKHIASCAALAATGLHSEELVAPVISGLPEDAC
                     ISAALQLQQDLPEQINLVSLGARGYSVMTRDRQGHVGYVENDKCSSGTGETIVKIAGR
                     FGLGLEEADQLAFGAAVSVPISARCSVFAKSEMTHFGNQGKPTDQLMKGYFESVARNT
                     AALLSRVRIEGPVYLMGGGARLASFVKAFREIAGGTVFVLDEPQLFEALGAAVLAADQ
                     YREDKLPKLPVDPEQLIIPRRTRFRIQSPAQQWASNVTYQSAPDVTEDPAVTPAVLGL
                     DLGSTGSKAVLTSIRTGNQLTDVYDRTRGNPVDASLRLLDEILRQTKPDVRAIALTGS
                     GREAAATVLRAVFPELAERIVVLNEIVAHATAAIRCDDQKGKSLSIVEIGGQDAKFIQ
                     IAGGQITESDMNKACSAGTGSFLEEQAVFYDIHDVKEFTRLAQKAKRPPDLGQMCTVF
                     IADAAAEAHNEGFSIEDLFGGFQYSVIHNYINRVMGGRTFGDRIFFQGKPASGPSLAW
                     TLAAVTGREVIVPHNPGAMGAWGIGLCAIRELGEEKLFAAGTFDLNLALNARVVSRIE
                     FRCADSKCHNFCHIDKTTVAVGAAEKTVLSGGACPKYEISSIASKKIPKEAPNALEER
                     MALLENFREAGTGDREVGVPYVGVLNGYLPWMVTFLSEIGFRVKVLRSDSKSLAGGEE
                     RCFSFDSCAPAKIAHAVADADVDTLVFPKFLNLHDRDGHGGRTCPMEQGMADMADQAL
                     KARGRNIRFVKPLLNLRDGLLNRRLIGRLRGLARELGVDDRHVKTAARRAAKAQQDYE
                     HALAEIGRRTLAYGRGQGIPVVAVIGSLHVIYDRAVNASIPDLLRQNGVLSLPMDCYP
                     IPDDIPELPRIVWEDANRALRVALSARNCGEVFPLFLSSFGCGPASFIEHFFRFLMEG
                     YPHTALESDGHGGFAGFVTRIQAFLHTVRQHDGRPVPAESSRLRLFDTLERPPLASQK
                     DSKFVVFTMGDQTAPIMAAVYRSWGYDAIPAIPTSAVSLAAGRRDCSGKECLAYQLIW
                     GSFRKYLDDNPPEKRTVLLQISGRGACRTCNFSMKDQISIENMGLSDRVSLRHVAAEL
                     ECPGLVSRFYTGTLVWNLLNQLAAYHRPYESEPGEVDRIYDDYCSDLIRILEKPSGRG
                     FSGIISKMGSVGRIVSGSIEQNQGSALFEQLVRRASTQFAAMRKCRTDQNSLRTIFLT
                     GDIYLRLDDFGSDAIIRRLNARGIRVIVEPLWVLAEYLVEERSAELFSLPQGTFMNAM
                     AKVLLPKSRKHFYEMAQEQNPWLPTFDVPEMLKKAKPIIDRHPRGEAPITVGSVIHHW
                     DKAVSDGIVIVGPWGCGPALIAESLLKHQRQIPTLFVYNDGSPLDERKLNGFVFRLRR
                     QEPKTATETGR"
     gene            complement(8125..8628)
                     /locus_tag="PLG98_07835"
     CDS             complement(8125..8628)
                     /locus_tag="PLG98_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33203.1"
                     /translation="MKKNKSKTKIILAALIVGMAVIAGIVYWHSGNDGDTGAAVDATQ
                     PVALNMSEQDIVDAEKNASIAAVSGETVAGTVTERPDYITEIEWQVLQSVTRQNPDIK
                     VNDLVNKMLFAKKKQAWLSAEKDSAERKLLAKQLLDMIPGQVAMEAIDGATAQTMEAE
                     LHADLLR"
     gene            complement(8770..10341)
                     /locus_tag="PLG98_07840"
     CDS             complement(8770..10341)
                     /locus_tag="PLG98_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HOE33204.1"
                     /translation="MGEPNVVTGTVLVPKSLYNYGLTARPIISYAVGTHGLVQSWAPS
                     IQLENGTDYENSNIAAALNRGYAVLVTDNPGYTNNDVPSYMVGKAQGQAVLDIVTAAL
                     QIPNISLKSYAKVGIWGYSQGGQSAAWAGQLQSTYAPGIKLAGVAAGGVPAELFSVGR
                     YLDGNNGSSFLLSTCIGLNAQYPNDILLNSRVNTDGMDAIGRGLSMGVFEALFAFMNT
                     PLSDFTKYGETSDQIFAIPEIAEVVNGQELGKTKINAPVFLYHGTGDEFIPLEQALNL
                     KEKYCSLGVNTSYMVYPGEHITTQFQAAPQVLDWLKDRFAGKSAASTCRTSNPRPAST
                     ANPVDGDFLFSLDGWKLDGMIKLKTLMTKVSLPEGSTFSAETNMTNNTITGGMDIPEF
                     SYYIYAFGLMPLQVKLKIVPAGTMTGTASLDKNGILHINGNVKADIYLKKVGELGIGI
                     PFSLKTKTPVDFPIVFDGPVSSLGDGSLTFTGTTTFPDMVENGIIINALFTGLMSGPG
                     QEFTFTVTPPAPVAW"
     gene            complement(10799..16180)
                     /locus_tag="PLG98_07845"
     CDS             complement(10799..16180)
                     /locus_tag="PLG98_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE33205.1"
                     /translation="MEKISDYVLEKKIHKTKNAVTYRGYKEKDFQSVIIKLLNTLHST
                     PSEIAQFRQEYELIQRLDIGSVVKTIDIIHYKGGLALILEDFDGIPVKDYFRPNEKIN
                     IASFLALSANIAATLAALHARGVIHRNIKPQSLLINPETEELKITDFGISALLTHEHD
                     EVYNPDFIRDTLTYMSPEQTGRMNRSVDYRTDLYSLGITLYEIVTGLLPFKSQDPMEL
                     IHAHIAMMPRAPVKLDAGVPAMVSDIIMRLLAKNPEARYQNGFGLQADFQECLKQWEE
                     KKRIEPFELGRQDISNRFIVPQKLFGREKEISELISRFEEVANGDKRLSVMVVAGAPG
                     IGKSAMINEIHKPIVARRGYFISGKYEQFRRDKPYSAIIQAFQVLVKQILSESEQRIN
                     LWKKNLLSTLGDNGRVITDVIPEVEWIIGKQPDLPVLGPEETRNRFKFIFEKFMSVFP
                     QQEHPVALFLDDLQWADMASLQLMKNIMLSDIHHLFILFSYRDNEVNETHPFSELLSE
                     VKKQNIRIDKITLDPLKEKEVTELIVNFLRCSEERGFGLAGLVQEKAGGNPFFVNQFL
                     HTLYNEKMIVHKGAQGWQWDVGEISRMKVADNLIQMMAGKIEKLPDNAQDVLKICACI
                     GNRFDLETLATVRKTSVDEALADLTEAIKEGMVSRLGDFYVFHHDRIHEAAYSLVTDA
                     EKSALHYKIGRILMGNLDEKELPGKLFYIVDQLNLGVQMIQNEEEREKLARFNLEAGK
                     KAKESAAYVPAYQYLKNGMNLLEENPWIKQYPLTLALYTETTEAAYLMGDYDKMNALA
                     EITLQHAKTNQEKTRIYTSRINACIAREDYRGAIDVAMPILNTIAPRFGIRLPKKANK
                     LHIALEIFRIMLKLAGKRSEDLINLPKTSDLEILAAGKILASVSHAAFYTDPNLLALI
                     ILKTFRLIFKYGLAPEHAFAFAGFGIVQAAGLWDFKSAIAFGKMGLKLVEKLDARDQA
                     ARTVFVYNALVRHWKYPMKDSIEPMIEGYKVGLETGDLGFATFNLYFTEVHYIFTGRE
                     LSDLEKYMERNNRIIAGMKQGHTLTLQSMTWQAILNLLGRCDNSVKLTGKAIDAEKLL
                     PSWEETENLAALSVFWFVKLILYSLFSEYKMALKASDEFRKYMDSQQGVLINKYAVLL
                     DSVARLKVYKNASLPEKIRHRVLLKINELKIRAWAKAGPMNCSYMYTAIKALYAWVIR
                     GNLAKAEKLVELAVRQCREYGDFVVEGIASEIMAGEYRSIGENEKAKRFLDRTGICYS
                     TWGATGLLNKIRKMYPELVSSEADVSLNVEATTAEKTKDDLSVALDLSTVMQVSQVIS
                     SEIMLERLLEKIMHMSVTSAGAQRGYLMLESEGKLLIEASEDIDNDETNVMQAMPLHD
                     CADICQAIVNYVHHSGKDVILGHAAKEGAFTDDSYINNKQCKSVLCIPIIHKGKISGI
                     LYMENNLSEDAFTPGRLDILRFISAQAAISIENAKLFELATTLENIEDGYWEIDLAGN
                     YTFLNNAVCQIHGYSKEELIGMNNRQYTDEETAKKVYKAFNNVFKTGQPLKGLDWQII
                     RKDGTKRYVEISILLQKDSSGKPIGFRGVVRDITERKNMEEKLQQTLESLSKAYDSTL
                     QVMVSAVEVRDPYTALHQVRSADVARDIAIEMGLPQEKIDAIRMAGAIHDIGKLSIPP
                     EILTKPEKLTSVEYALIKEHSRSGYEVLKNVESPWPLAKIVHQHHERMDGSGYPRNLK
                     GEEILLEARILAVADVISAMVSHRQHRPSLSIEVALEEIEKNKGTLYDNSVADACLRL
                     FREKGYKLN"
     gene            16637..>17871
                     /locus_tag="PLG98_07850"
     CDS             16637..>17871
                     /locus_tag="PLG98_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOE33206.1"
                     /translation="MRNSVDGKNIVIIGSGIGGLSTGIILSLMNFHVTVVEKNPLPGG
                     LMRSYRRGGIDCPVGIHYVGALGAEEPLGKMFAALGIPVDELFVPMAGEGIVDRYIFD
                     DLTFDLPDGIDTLENSLRDTFLQEKPALDVLMKNLRDISRQMTDPSFLFAQGDPFQNM
                     GYYQPMGELLDSLNVSPALRAVLSVPCNLIGVPLNDCPVIFHHMVLASYLFSSWRLKE
                     CGRKMTDVFIRRFEDLGGQLVLHDGVKKILFEKGKVSGVILESARTLFADAVVAAIHP
                     KVMLQLLDENVLRDSHRRRILGLKETESVIAVQVSVDAAKHPEMHHNIYRLHRDENGV
                     IQEGVFYQVQRTTSAGLHLLSIITKSLFGDWSRWENTVTGQRGSEYEEHKMNLAKGLL
                     KKAEDVFGDLNDARILDVYT"
BASE COUNT         4381 a         4400 c         4337 g         4653 t
ORIGIN      
        1 gtttgccggt cgatccgccg gtaaaaagcc gaagaaacgt ttcatccgga tggatgagcg
       61 attcggaacg gagggtttcc ggggcagcgg gaaccggcgt tatgatttca accccctccg
      121 gcatttcttc cggatggtcg gcaatggcaa aattaaaacc ggtggcttcg gacatttctt
      181 tcatggcatg agtggaaaaa gaataaggca aaatcacgtt gcaggcaccg gtcagcgacg
      241 ccatgacaca ggcggcggtc accgctttat tcgcactgca caaacaaata ttttttttgc
      301 cgctgcgcct gtccaggatc ttcttcaggc cggccgccag cgccagaaca tcgccgtaac
      361 tgtatccgcc gaaggtgaac tcttttcggc agacgctcgg ggaaagttcc agcatttttt
      421 tgtacaggcc gacaaaagga tctttttttg cttgcgtcat catcaggcag ctcaagaatt
      481 ttttattcct ttaccgtaaa aaggaggaaa aagcacctat tatcaaagcc ggtataattc
      541 ttgttgactt ccgcatgttt ttttcattag tttaagaaaa aatttgatga catcaaccca
      601 tgcttgacat cgatattgac agtttgattc cgcaccgcaa aaagataaaa attatcaatc
      661 acgtcattga tatacaggag aattctgctg tgagcgccgc gatggtcggt ccggactggc
      721 cccgttccga tggaaaaacc gttgatgcgc tggtgttgat cgaaggaatt gcgcaaacgg
      781 ctgccattgt ggaaggatac aagaggaaac agcggggtgt cggcgccgtc aagggctggc
      841 tggtcggtat caaaaatgcc gcgttttatg aagagaaaat accgctgaat acgcatctgg
      901 ttattctgct ggaaaacaag gatgcttttg ataattacgg cgtcgtagaa ggaacggtta
      961 aaacgggaga aaaaattctt gcaacagccg tccttcaggc cgtgcgtctc aatgataacg
     1021 atcaataatt ttgttacggg gagtaaaaaa tatgaacgat aaaaaaacag cgattatcac
     1081 cggtgcaagc cgagggatag gacgcgccac ggcagtgcaa ctggcgcggg ccggatattt
     1141 cgtcatcatc aacttcaaga ccaatgagac ggcggcgcag gaaacattga atatggtgaa
     1201 atcagaaggt ggagatggca ctatcattcc attcgatgtc gccgattcgg aacagaccgg
     1261 aaatgcggtt aaggcgatca ccgaacaata taaaaatatc caggtgctca ttaacaacgc
     1321 cggcattacc gctgacgggc tgtttatgtt actgggcgaa gaggaatggg gcagtgtcat
     1381 caacaccagc ttgaatggtt tttatcatat gaccaaaccc attgtgcgtg aaatgatcag
     1441 aaaaaaacgc ggctccattg tttccgtttc ctctctttcg gcgttgatgc ccaatcgcgg
     1501 acaggccaac tacgcggccg cgaaagccgg aattattgcc gcgtcgcgtt cacttgcgtc
     1561 cgaagtggcc cgtttcggag tccgggtcaa tgtcgtcgct cccggcttga ttgaaacgga
     1621 tatgatcaaa gacgctccgg ttgatcagat caaacaaatc attcccatgg ccaggttggg
     1681 aaaaccgcag gaagttgcca gcgtcatccg ctttttatgt tccgatgacg cttcttacgt
     1741 aaccgggcag gtcatcggca ttaatggtgg aatatgctga tgcgcctata ccataagata
     1801 tctctgttca cgatccttct gacaggattc tgtttcctgt acggcaatgt ttgtcatgcg
     1861 gaagaaaaag gtttagaaaa gctccgcaaa gaatcggtaa gaatcaaaac gcttcaggca
     1921 gatttcacgc aaaaaaaaag catgaaaatt ctatccagac cacttgtttc tcagggttat
     1981 ttttattttg caatgcctga ttctttgcgt tgggnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnccactt gtttctcagg gttattttta ttttgcaatg cctgattctt
     2161 tgcgttggga atacattaag cctctgaaaa gtgttgtgct cagtcacaac cagcaggcca
     2221 ggcgctatat gcattccgat gggaaatggg tcgaagataa aacaaatggc gtgcaggcca
     2281 tgagaatcgt tctgaatgaa attgccggat ggatgaacgg cagattcgat cagaatccat
     2341 catttaaagc aaccgtcatc gggaaagaga atacctgcat catcctgaca ccggtgggaa
     2401 aaaaaatgaa aggaatgatt gaacgcatcg aaattaatct cgcgccccaa gcgcgggttg
     2461 tccaatcggt caaaattctg gaaggacccg ataacatcac ccagatcaat ttcagtaatg
     2521 ttaaaatgaa tcacgcgatc gatccgacca tttttaagga tgtacgatga ggcgacatct
     2581 tctttggata gtgatttttt ttctgtcttc ctgccagacc ctgcccatca taaatgcacc
     2641 ttcttctcca cgtaaagaaa aatcgttcgt ttgccctgat ccttttttaa agagaaatta
     2701 tcgtcttgtg catgccatcg aggcccgtct tgacggaaga gtacaaagtc agattatcgg
     2761 cataacgctg gctctccccg atacccgttc ggtctcctgc gcgattataa cggtggaagg
     2821 catgtcgctg tttgaagccg aagcaaaccc tcatgggatc aacgtcaaac gagccctacc
     2881 tccctttgat tccaaagatt tcgccaacaa tatgatggag gatattaagc tgatattttt
     2941 agcaccggaa ggcgttttac aaaacagagg ataccttgcc gacggatcga aagcctgtcg
     3001 ttaccgtcaa gaaaatggcg gctggattga cgtcatagaa aacgaatcgg gagaaacaca
     3061 aatcaaacgt tactcttcct ccgaagtttt aaaaagggat gtccgtttca gcggtaccca
     3121 taagcaactt tatcaacgca tcggcctgca ggcagacgca aaatttgatt attttctgtt
     3181 gatgaatttg atcgaagatc aacccataaa aaaacaagcg attaaaagaa attaaaccgt
     3241 tattaagatt tgaataattt tattccgcga ttcaaaacac atccgggaag catccgtcat
     3301 cgccaatatg tttccgttgt gaccgttttt tttcggggta aatttccttg acacaatcgc
     3361 ggatgttcct tataaccccc gttcctaaaa gaaggaaaat cttatcgatg ttgttttttt
     3421 ctgaaattgt cgaattcaag cggagaaaaa aatgaagagc catcatttac atcataaagg
     3481 gaaatcgttt tcgggaaaac aaggagaaac cgtcatggtt gccggctgtg atctgggcaa
     3541 atccaccgcg aaatttgtga tgctcaacgt ctgtgacagc gacacaccga tcctggaaga
     3601 tttccgctgc atcgtccatc atgggaatcc gctcaatgct ttccgcgaat ggtaccgtga
     3661 aaagcatatc gcttcatgtg cagctctcgc cgctaccggt ctgcactccg aggaactggt
     3721 cgctccggtg atttccggac tgccggaaga cgcttgtatt tcggcggcgc tgcaattgca
     3781 gcaagatctg ccggaacaga ttaatctcgt aagtctcggc gcccgcggat attcggtcat
     3841 gaccagagat cggcagggac atgtcggcta tgtggagaat gacaaatgtt cctctggaac
     3901 gggcgagacc attgtaaaaa tagccggccg tttcggactc ggtctcgaag aagccgatca
     3961 actggctttc ggcgctgcag tcagcgttcc catctcggcc cgttgttcgg tctttgccaa
     4021 aagcgaaatg acccatttcg gtaaccaggg gaaaccgacc gaccagctca tgaagggtta
     4081 tttcgaatcg gttgcccgca acacggcagc ccttctgtcg agggtacgca tcgaaggccc
     4141 cgtttatctt atgggaggcg gtgcgcgact cgcttctttt gtcaaggcat tccgggaaat
     4201 cgccggcggc acggtttttg tgcttgatga accgcaactt ttcgaggccc tcggtgcagc
     4261 ggtacttgca gccgaccagt accgggaaga caagctccca aaactgcctg ttgatcccga
     4321 gcagctaatc attccgcggc gaacaagatt tcgcattcag tcgccggctc agcagtgggc
     4381 atcgaacgtc acgtatcagt cagcccccga tgtcacggag gatccggcag tcacaccggc
     4441 cgttctaggc cttgatctgg gatcgacggg cagcaaggcg gtgctaacgt ccattagaac
     4501 cggcaaccag ttgacagacg tttatgaccg tactcgcggg aatccggttg atgcttcgct
     4561 gcgtctgctg gacgagattc tcaggcagac gaaaccggac gtacgggcta ttgccctgac
     4621 cggctccgga cgtgaagccg ccgcaacggt tctccgcgcg gtgttccctg agcttgccga
     4681 gcggattgta gttctgaacg aaattgtcgc ccatgcaaca gctgcgatca gatgcgatga
     4741 ccagaaaggg aaaagtctct ccatcgttga aatcggagga caggatgcca agttcatcca
     4801 gatcgccggc ggtcagatta ccgaaagcga catgaacaag gcatgcagtg ccggaaccgg
     4861 ttctttcctc gaagaacagg ccgtttttta cgatatacac gatgtaaaag agttcacaag
     4921 gctggcccaa aaagcaaagc gtccgccgga tctcggacag atgtgtaccg ttttcattgc
     4981 cgatgctgcc gctgaagcgc acaacgaagg attcagcatc gaagatttgt tcggcgggtt
     5041 ccagtattcg gtcatccaca actatatcaa tcgggtcatg ggaggacgga ccttcggcga
     5101 ccggatcttt tttcagggca agccagccag cggaccgtcg cttgcctgga ctctggccgc
     5161 ggtcacgggt cgggaggtaa tcgttccgca taatcccggg gccatgggcg cttggggcat
     5221 cggtctgtgc gcaatccggg agctgggcga agaaaaactt ttcgcagccg gcactttcga
     5281 tctgaatctg gcgctcaacg ccagagtggt ttccagaata gaattccggt gtgccgattc
     5341 caaatgtcat aatttttgtc atattgataa aacgactgtc gctgtcggcg cagcagaaaa
     5401 gaccgttctg tccggaggcg cctgcccgaa atacgaaatc tcatcaatcg cttcaaagaa
     5461 aattcccaag gaggccccga acgcgcttga agaaagaatg gcgctgctgg aaaatttccg
     5521 ggaagccggg acaggcgatc gggaggtggg ggtgccttat gtcggagtgc tgaacggata
     5581 cctgccctgg atggtgactt ttctgtcgga aatcggattc cgtgtaaagg tgttacgctc
     5641 tgacagcaaa tcgctggcgg gcggtgaaga acgctgtttt tcgttcgatt cctgcgctcc
     5701 tgctaaaatc gcccacgcgg tggcggatgc ggatgttgac acgcttgtat tcccgaaatt
     5761 tctgaatctg catgacaggg acggccatgg cggccgcacg tgtccgatgg agcagggcat
     5821 ggcggacatg gccgaccagg cgctgaaagc tcgcggccgg aatatcaggt tcgtcaagcc
     5881 ccttctcaac ctgagagacg gcctgctgaa ccggcgactc atcggccgcc ttcgagggct
     5941 cgcccgggaa ctgggcgtcg atgatcgaca tgtcaaaaca gcggcccgcc gcgcggcaaa
     6001 agcgcagcag gactatgaac atgcgctggc ggaaatcggg cggcgaaccc tggcctacgg
     6061 ccggggacag ggaattccgg tggttgccgt catcggttcg ctgcatgtga tctatgatcg
     6121 cgcggtcaat gccagtattc cagatcttct caggcaaaac ggcgtactgt cgctgcccat
     6181 ggactgttat ccgattccgg acgatatccc tgaacttccc cgcatcgttt gggaagatgc
     6241 gaacagggct ctccgtgtcg cgctttcagc caggaattgc ggagaggtgt tccccctttt
     6301 tctttcctct ttcggctgcg gaccggcctc gtttattgaa catttctttc ggttcctgat
     6361 ggaaggttat ccgcatacgg ctctggaaag cgacgggcac ggaggctttg cgggtttcgt
     6421 gacaagaatc caggctttcc tgcacaccgt tcgtcagcat gacggccggc cggttcccgc
     6481 agaatcgtca agactccgcc tgttcgacac actggaacgc ccgccgcttg catcgcaaaa
     6541 ggattcaaaa tttgtcgtct ttaccatggg cgaccagacg gctccgatta tggcggctgt
     6601 ctaccggtcg tggggctatg acgcgatccc ggcaattccc acatcagcag tgtctctggc
     6661 tgcggggaga agagactgtt caggcaaaga gtgccttgcc taccagttga tctggggttc
     6721 gtttcggaaa tatctcgatg acaacccgcc cgagaaacgc acggtcctgc ttcaaatcag
     6781 cggaaggggc gcctgccgca cctgcaattt ctccatgaaa gatcagatat cgatcgagaa
     6841 tatgggcctt tcagatcgtg tgtcgctgcg ccatgttgcc gccgagctgg aatgccccgg
     6901 gttggtcagc cggttttaca cgggaacgct tgtatggaat cttctgaacc agcttgctgc
     6961 ctatcaccgc ccttatgaat ccgaaccggg agaagtcgac aggatatacg atgactattg
     7021 cagcgacctt atccggatac tggaaaaacc ttccggaaga ggtttctccg gaatcatttc
     7081 aaagatggga tctgtcggac ggatcgtctc cggaagtatt gagcagaacc aaggcagcgc
     7141 tcttttcgag caacttgttc gccgcgcatc gacacagttt gcagccatgc ggaagtgtcg
     7201 gacggatcaa aattctctcc ggacgatttt ccttacggga gatatctatc tgcgtctgga
     7261 tgatttcgga agcgacgcca ttatccgacg gctgaatgct cgcgggatac gggtcatcgt
     7321 ggaaccttta tgggtactgg ccgaatatct ggttgaagaa cgttccgccg aattattcag
     7381 tctgcctcag ggaactttca tgaatgccat ggcgaaggtc ctcctgccaa aatcgcgaaa
     7441 acacttttac gaaatggctc aggaacaaaa cccctggctg cccacattcg atgttccgga
     7501 aatgctgaaa aaagcaaaac cgatcatcga ccggcacccg cgtggcgagg ccccgataac
     7561 cgtgggaagc gttattcatc actgggataa ggcggtctca gacggtatcg tgattgttgg
     7621 accatggggg tgcggtccgg cactgattgc cgaaagcctg ctcaaacatc agcgccagat
     7681 tccgacgctt ttcgtttata acgacgggtc tcccctcgac gaacgaaagc ttaatggatt
     7741 cgtattccgg ttacgcaggc aagaacccaa aacggcaaca gaaaccggcc ggtaagaaaa
     7801 tctccgcctc atgagagtct ttcttgatgc ctgtgtgaaa aaaatcaacg gggctatccc
     7861 ggtcggacag cctctttcat cgtttattca atccgtgtaa gtttagaatc cccttgcctt
     7921 aaaatttttg gcaaccttac cgtaaaattc ttttgcatcc ccgtcagggg caataccgaa
     7981 aacgcaattc atctcacaga aatgattgat gcccggatac aacaaagacg tgctcatgat
     8041 gccgaggaga acaacctgac cgatttccct tttccaacaa actctaaagt gtccccggat
     8101 ttcggatttg gatttgcgga tcttttatcg caacaagtcc gcatgaagtt ccgcttccat
     8161 tgtttgggct gttgcaccat caatggcctc catagcaact tgacccggaa tcatatccag
     8221 caattgcttg gccagaagtt tgcgctcggc tgaatctttt tcggcagaaa gccatgcctg
     8281 cttttttttg gcaaacaaca ttttgttgac caaatcgttt actttgatat ccggattttg
     8341 cctggttacg ctttgaagca cctgccactc gatttcggtt atataatcag gtctttctgt
     8401 taccgtaccg gcaaccgttt ctcccgaaac agcagctatg gacgcatttt tctcagcgtc
     8461 cacgatgtct tgttcagaca tgtttaatgc gacgggttgt gttgcatcaa cagcggcccc
     8521 ggtatcgccg tcattgccgc tatgccaata cacaatgccc gcaatcactg ccattccgac
     8581 tatcagcgca gcgagtatta tttttgtctt ggacttattt tttttcatat cgcttcgggt
     8641 catttataga ctctccttgt gtcattgcct tgttacgata ttaaaatatc atatcgtgaa
     8701 aatatatctc cggccaggcc attgtcaggc agcctgaccg gagatgtgaa aaaaatcaga
     8761 taaaaaatct taccacgcta ccggcgcagg cggcgtcacg gtgaaggtaa attcctgacc
     8821 gggaccggac atcaaacccg taaacagggc attgataata atcccatttt cgaccatgtc
     8881 cgggaatgtc gtggttcccg tgaatgtcag agaaccatct cccagcgatg atacaggtcc
     8941 gtcgaagacg attgggaagt caacaggcgt tttggttttc agcgagaagg gaatcccgat
     9001 tcccagttca ccgacctttt tcagataaat gtctgctttg acatttccgt tgatatgcag
     9061 gattccgttt ttatcgagtg aggctgtgcc tgtcatggtt cctgcgggca cgatcttcaa
     9121 tttcacctgc aacggcatca aaccaaacgc gtagatgtag taactgaatt ccgggatgtc
     9181 cataccgccc gtaatcgtat tattcgtcat attcgtttcc gccgagaacg ttgatccttc
     9241 cggtaaagat actttcgtca tgagtgtttt cagtttgatc atgccgtcaa gtttccaacc
     9301 atcgagagaa aaaagaaagt cgccgtcaac gggattggct gtggatgccg ggcgagggtt
     9361 ggaagttctg caggtactgg ctgcgctttt cccggcaaac cggtccttca accagtcaag
     9421 cacctgtggt gcggcctgaa attgcgtcgt gatatgttcg ccgggataaa ccatatagga
     9481 tgtattgacg ccaaggctgc agtatttctc tttgagattc agagcctgtt ccagcgggat
     9541 aaattcatcc cccgtgccat gatacagaaa aacgggtgca ttaatttttg tttttccgag
     9601 ttcctgacca ttgaccactt cagcaatttc cggaatcgca aaaatctgat cggaggtttc
     9661 cccgtatttg gtgaaatcgg aaagcggcgt attcatgaaa gcaaaaagag cctcgaatac
     9721 acccatggaa aggcctcgac caatcgcgtc catcccgtca gtgttcacac ggctattcag
     9781 aagaatgtca ttcgggtact gcgcgttcag cccgatgcag gttgacagca ggaaggatga
     9841 cccgttgttg ccgtccagat atctcccgac agaaaacagc tcggcaggaa caccgccggc
     9901 cgcaaccccg gccaatttaa taccgggtgc gtaggtcgac tgcaactggc ctgcccatgc
     9961 cgccgactga ccgccctggg aatatcccca gataccgact tttgcatagg acttgagaga
    10021 tatattgggg atctgcagag ctgccgtcac aatatcgaga actgcctgtc cctgagcctt
    10081 gcccaccatg taggaaggta catcgttgtt ggtgtaaccc gggttatcgg tgacgagaac
    10141 ggcatagccc ctgttgaggg cagccgcgat attcgaattc tcgtaatccg ttccgttttc
    10201 cagttgaatc gatggcgccc aactctgcac aagaccgtgc gtgccgaccg cataactgat
    10261 gatgggacgc gcggtgaggc cgtaattata taaagacttc ggaaccagta ccgtaccggt
    10321 aaccacgttg ggttctccca aagcatcctg tgacgcatac atgacggtcc atgaataaca
    10381 cgatggtccg cccggcggaa cggatgttga tgtacggtaa cggattaagg tgccatgcgt
    10441 gccgggtata gtggatggct tctcataaaa agccattccg gctggaccct gtgtgggagc
    10501 agcaactgat gaaatcgccc aaaaagctcc taccatcaaa atcaaaaagg ataaaaaaaa
    10561 cagtcgttta taccaaaaca tacgtttcat aaaattcccc ccttaaaatt ttttttgaat
    10621 aatgaagaag tgcgacccat acatcaactt tgacatcttg atgttcttac atgggtctca
    10681 tttggtcaga aaacgtgcag gcgagaatca gaaacgtacc gttcgaatcg cctgttaaaa
    10741 tattatattt tgtttttttg tcaagaaaaa aatgatttaa atggtttgat aaccttaatt
    10801 aattgagttt ataacccttt tcccggaaca atctcaggca ggcatccgct accgaattat
    10861 cataaagagt tcctttattc ttttcaatct cttcaagggc cacctcgata ctcaaagacg
    10921 ggcgatgttg acgatgcgag accatggcag aaataacatc ggctacagca agaattcttg
    10981 cctccagaag aatttcctct cctttaaggt ttctgggata acccgatccg tccatccgtt
    11041 catggtgctg gtgaacaatt ttcgccagcg gccagggaga ttccacgttc ttcaagacct
    11101 catagccgct gcgggaatgc tctttaatta aagcatattc aacactggtt aacttttccg
    11161 gtttggttaa tatttcaggc gggatggata acttgccgat gtcgtgaatg gccccggcca
    11221 tgcggatcgc atcaattttt tcctgaggca gacccatttc gatggcgata tcccgcgcca
    11281 catccgccga ccggacctgg tgcaatgcgg tgtagggatc cctgacttcc acggcggaca
    11341 ccatgacctg aagcgtgctg tcgtaggctt tgctcaggct ttccagcgtt tgttgcagtt
    11401 tctcctccat atttttgcgt tcagtgatat cacggacgac ccctctgaat ccgatcggtt
    11461 tgccggacga atctttctgc aataaaatgg atatttcgac gtatcttttg gttccgtcct
    11521 tccggataat ctgccagtcc agccccttga gcggctgtcc cgttttgaaa acattattga
    11581 aggctttgta aacttttttt gcggtttctt catccgtata ctgccgattg ttcatcccta
    11641 tcaattcctc tttcgagtag ccatggatct gacagacggc gttattcaaa aatgtataat
    11701 tgccggccag atcgatttcc caataaccgt cttcgatgtt ttccagggtt gtggccagtt
    11761 cgaagagctt ggcgttttca atagagatgg ccgcctgggc ggagatgaaa cggagaatgt
    11821 ccagtcgccc gggggtaaat gcatcttcgc tcagattgtt ttccatatat agaattccgg
    11881 aaattttacc tttatgaata atgggaatac acagcacgga tttacactgc ttgttgttga
    11941 tataggaatc gtccgtaaag gctccttctt ttgcggcatg gccgaggatg acatctttcc
    12001 cgctgtgatg gacgtagttg acaatcgcct ggcaaatatc ggcacagtca tgcagcggca
    12061 tggcctgcat gacgtttgtt tcatcattgt caatatcttc gcttgcctca atgagtaatt
    12121 ttccttctga ctccagcatg agataaccgc gctgggcgcc ggcgctggta acggacatgt
    12181 gcatgatttt ttccagaagc cgctccagca tgatttcact ggaaatcacc tgggagacct
    12241 gcatcactgt ggacaggtcc agtgcaaccg acagatcatc ttttgttttt tcggctgtcg
    12301 ttgcctcaac attcagcgaa acatccgctt cagaggaaac cagttccgga tacattttcc
    12361 tgattttatt cagcaggccc gtcgctcccc atgtggaata gcagatccct gtccgatcca
    12421 ggaatctttt tgccttttca ttctctccga tcgatcgata ttccccggcc atgatttcag
    12481 aggcaatgcc ctccacaaca aaatcgccgt attcccggca ctgcctgacg gccagttcca
    12541 ccaatttttc cgctttcgcc agattgccgc gtataaccca tgcatacaaa gctttgatag
    12601 ccgtatacat gtatgaacag ttcatgggcc cggcttttgc ccacgcccgt attttcaact
    12661 cgttgatttt cagtaatacg cgatgtctga ttttctccgg cagggaagca tttttataca
    12721 ccttcagcct ggctacagaa tccagtaata cagcatattt attgattaaa actccctgct
    12781 gcgagtccat gtattttctg aattcatcag aggctttcaa tgccatcttg tactcgctga
    12841 aaagcgaata caagattaat ttgacgaacc agaaaaccga aagagccgcc agattttcag
    12901 tttcttccca tgagggcaga agcttttccg cgtcgatggc tttaccggtc aatttcacgg
    12961 aattatcaca tcgtcccagc aaattcagaa tcgcctgcca ggtcatcgat tgcaatgtta
    13021 atgtatgtcc ctgtttcata ccggcgatga ttctgttgtt tctttccatg tatttttcca
    13081 gatcggaaag ttccctgccg gtgaagatgt agtgtacttc tgtgaaataa aggttgaaag
    13141 tggcaaatcc cagatcgcct gtctccagtc cgaccttata gccttcgatc ataggctcaa
    13201 tgctgtcttt catgggatat ttccagtgtc ggaccagcgc gttatacaca aaaaccgttc
    13261 gggctgcctg atcgcgggca tccagttttt ccacaagttt cagtcccatt ttgccgaagg
    13321 cgatagcgga tttgaaatcc cagagtcctg ccgcctgaac gattccaaaa ccggcaaacg
    13381 caaaggcatg ttccggcgcc aggccgtatt tgaatatcag ccggaaggtt ttcagaatga
    13441 ttaaagccag caaattcggg tcggtgtaaa aagcggcatg gctcacactg gccagaattt
    13501 ttccggctgc caggatttca agatcacttg ttttgggcag attaatcaga tcttccgatc
    13561 ttttccctgc aagtttgagc atgattctga atatttccaa agcgatatga agtttattgg
    13621 ctttcttcgg cagtcggata ccgaaccggg gcgctatggt attcagaatc ggcatcgcca
    13681 cgtcgatggc tccccggtag tcctcacgcg cgatacaggc attaatcctc gatgtgtaaa
    13741 ttctggtttt ctcctgattt gtctttgcgt gctgcaacgt gatttccgcc agcgcattca
    13801 tcttgtcgta atcacccatc aggtaggcag cttccgtcgt ttcggtgtaa agggcaagtg
    13861 taagagggta ttgttttatc cagggatttt cttccagaag attcattccg tttttcagat
    13921 actgatacgc gggcacatag gccgccgatt ctttcgcctt ctttccggct tccagattaa
    13981 accgcgcaag tttttccctc tcttcctcgt tttgtatcat ctgaacgcca agattgagtt
    14041 gatcaacaat gtagaacagt ttgcccggca gttccttttc gtctaaattt cccatcagga
    14101 tcctgccgat tttgtaatga agcgccgact tttccgcgtc cgttaccagc gaataggctg
    14161 cctcatgaat ccggtcatga tgaaagacat agaaatcgcc gagccggctg accattccct
    14221 ctttaatggc ttcggtaaga tcggccagcg cctcatccac cgatgttttt cttactgtcg
    14281 ccaatgtttc cagatcaaaa cggttgccga tgcaggcgca gattttcaaa acatcctgag
    14341 cattgtcggg aagtttttca atctttccgg ccatcatttg aatgagatta tccgccactt
    14401 tcatccggct gatttctccc acatcccatt gccagccctg cgctcccttg tgaacaatca
    14461 tcttttcatt gtaaagcgta tgaaggaatt gattcacaaa gaaaggattg ccgccggctt
    14521 tttcctgcac aagcccggcc agtccgaatc cccgttcctc cgaacaccgt aaaaagttga
    14581 cgattaattc cgtgacttcc ttttccttca agggatcgag tgtgattttg tcgatacgga
    14641 tgttctgctt tttaacttcg ctgagaagtt cgctaaaggg atgagtctca ttcacctcat
    14701 tatccctgta tgaaaataga atgaagagat gatggatgtc gctgagcatg atgtttttca
    14761 tgagttggag actcgccata tccgcccact gcaaatcatc cagaaaaaga gccaccggat
    14821 gttcctgttg cggaaagaca ctcatgaatt tttcaaagat aaatttaaac cggttccggg
    14881 tctcttccgg acccagaacc ggcaaatccg gctgctttcc gatgatccat tcgacttccg
    14941 gaatcacgtc cgtgatcacc cgaccgttgt cgcccagcgt acttaaaaga tttttcttcc
    15001 agagattgat tctttgttcg ctttccgaca atatttgttt cacgagaacc tgaaaagcct
    15061 gaataatggc gctgtaaggt ttgtcccttc tgaattgctc atatttgccg gaaataaaat
    15121 agccccgcct agccacgatc ggtttatgga tttcattaat catggccgat ttgccgatac
    15181 cgggagcacc ggcaacaacc atcacggaca atctcttgtc gccgttggct acctcctcga
    15241 atctggagat cagttccgat atttcctttt cccgtccgaa aagtttttgc ggaacgatga
    15301 accggttgga tatatcctgt ctccccagtt caaagggttc tatccttttt ttctcttccc
    15361 attgtttcaa gcattcctga aaatcggctt gaagaccgaa accgttctgg tatctggcct
    15421 cggggttttt ggccagcagc ctcatgatga tatccgacac catggcagga acaccggcat
    15481 caagcttaac gggcgccctg ggcatcatgg cgatatgcgc atgaatgagt tccatggggt
    15541 cctgcgactt gaaaggcagc aaaccggtga cgatttcata cagagtgatt cccagcgaat
    15601 agagatccgt gcggtaatca acagacctgt tcatccggcc ggtctgctca ggcgacatgt
    15661 atgtcagggt atcgcggatg aaatcaggat tatagacttc atcatgttcg tgagtcagca
    15721 gagcggatat tccgaagtcc gtaattttca attcctccgt ttccggattg atcagaaggc
    15781 tttggggttt tatgttccgg tgaatgactc cccgggcatg cagcgctgcc agtgttgcgg
    15841 caatgttcgc cgatagggcg agaaatgatg cgatgttgat tttttcattc gggcggaaat
    15901 agtccttaac gggaataccg tcaaaatcct cgaggatgag cgccaggccg cctttgtaat
    15961 gaataatatc gatggttttg acaacgcttc cgatatcaag acgttgaatc agttcgtatt
    16021 cctgccggaa ttgtgcaatt tccgaagggg ttgaatgaag ggtgttcagg agtttaataa
    16081 taacggattg aaaatctttt tccttataac cccggtacgt aacggcattt ttcgttttgt
    16141 gtatcttttt ttccagaaca taatcgctta ttttttccat aaatgtattt ctcttctata
    16201 ttttcaacgg ctgttaagaa acattaaaaa ttttatcgta tagggattgg tcacatcatc
    16261 attcaaatac tccgcggctt tttccttctc gaaatcaaca tactaataat gggttttgat
    16321 atcttttaat gggattctgg aatttttatc aaagacataa tcctgttggg cgtgctcgcc
    16381 cgtcaacctg tttttgataa ccggtgccgc aacaatgcca agaacgatgg cgaccgcgaa
    16441 cgcagcaatc cttttcttat tgatataaga aatcccccca acggatatgt gccgcgcttt
    16501 ttttctggaa tggttaaagt atagaaataa ctttccccga tgtcaatttg tttttatccc
    16561 ccggcagacg aaaggatcat ttccggttga cttatagaaa acaaatgcat acagaaaaac
    16621 aaaaaggggc aagcagatga gaaatagcgt cgatggcaag aacatcgtga tcatcggttc
    16681 cggtatcggc ggattgagta ccgggattat tttatctttg atgaattttc acgtcacggt
    16741 cgtggaaaaa aatccgctgc ccggcggatt gatgcggtct taccggcgcg gcggaataga
    16801 ctgccctgtc gggattcact acgtcggcgc attgggggcg gaagaacccc tcggtaaaat
    16861 gtttgcggcc ttgggaatcc cggttgacga gctatttgtt cccatggctg gagaaggcat
    16921 cgtcgaccgc tatattttcg acgatttaac cttcgattta cccgacggaa tcgacacgct
    16981 ggaaaacagt ctgagagata cgttccttca ggaaaaaccg gcgctggatg tccttatgaa
    17041 aaatctgcgc gacatttccc ggcaaatgac ggatccctcg ttcttgttcg ctcagggtga
    17101 tccctttcag aatatgggct attaccagcc gatgggagaa ttactggatt ctttgaacgt
    17161 atcaccggcc ttgcgtgccg ttttatcggt tccctgtaat cttatcggcg tcccgctcaa
    17221 cgattgtccg gtcattttcc atcacatggt gctagccagt tatctttttt cttcgtggcg
    17281 actgaaggaa tgcggccgta aaatgacgga tgtctttatc cgccgctttg aagacctcgg
    17341 ggggcaactt gtcctgcacg acggcgtcaa gaaaatcttg tttgaaaagg gtaaagtaag
    17401 cggagtgatt ttggaatcgg ccagaacgct tttcgccgat gcggtggttg ccgctataca
    17461 cccgaaggtc atgcttcaat tgctggacga aaatgttctg cgcgattctc atcgccggcg
    17521 cattctggga cttaaggaaa cggaaagcgt tatcgcggtt caggtctctg ttgacgccgc
    17581 caagcatcct gaaatgcatc ataacattta caggctccac cgtgatgaaa acggtgtgat
    17641 tcaggaaggc gtattttatc aagtgcaacg cacgacctcc gcaggccttc atctgctttc
    17701 gattattacc aaatccctct tcggtgattg gagtcgatgg gaaaataccg tgacaggcca
    17761 acgcggcagc gagtatgaag agcacaaaat gaacctcgcc aaaggcctgc ttaaaaaggc
    17821 tgaagacgtc ttcggcgatt tgaacgatgc ccggattctg gatgtataca c
//
LOCUS       DAOSME010000047        17524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_3866_length_17524_cov_24.125995, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000047 DAOSME010000000
VERSION     DAOSME010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 17524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17524
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3866_length_17524_cov_24.125995"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1663)
                     /locus_tag="PLG98_07855"
     CDS             complement(50..1663)
                     /locus_tag="PLG98_07855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOE33207.1"
                     /translation="MAKDKNNGGANLGFEADLWRAADALRSNMDAAEYKHVVLGLIFL
                     KYISDAFEEQHAKLEAEKTQGADPEDPDEYRAVNIFWVPKEARWSHLKGSAKQPTIGK
                     TVDDAMLAIERDNSSLKGVLPKDYAHPRLDKQRLGQLIDLIGNIGLGDRENRSKDILG
                     RVYEYFLSQFASAEGKKGGQFYTPRCVVRILVEMLAPYKGRVYDPCCGSGGMFVQSEK
                     FIEEHGGRVGDIAIYGQESNHTTWRLAKMNLAIRGIDSNLGKEHADSFHRDLHPDLKA
                     DFVLANPPFNDSDWRGELLKDDKRWKYGTPPASNANYAWVQHFIYHLAPNGIAGFVLA
                     NGSMSSNQSGEGEIRKSIIEADIVDCMVALPGQLFYSTQIPVCLWFISRDKKNGRFRD
                     RRGETLFIDACKMGTLIDRVHRELTDEDILKIAETYHAWRGLPAEASAQAGGDTAKSR
                     HSGGGRNPERYQDIPGFCKSAKLDDIRGHNYILTPGRYVGAAEVEDDGEPFEDKMSRL
                     TAELKKQMEEGAILDKQIWQNLKEIGYGK"
     gene            2041..2874
                     /locus_tag="PLG98_07860"
     CDS             2041..2874
                     /locus_tag="PLG98_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOE33208.1"
                     /translation="MAEKNLVYRGKSKDVYKITGGKYKGKYRFVFTDRGTGYIEKGKV
                     VFDPGYDVVVGEIPGKGAIACRFATYFFKLLKAKGVATHYIETISGNEMIVEPAIPLS
                     MKEAAPQFAGSAPLTNLEFTWRNNATGSFWRRYPFVKPCVNLHKVVEAWTKGDSDILI
                     TMEALEETGALTKKEITDSVKLVKKIAEIVSNEFTSKKLHVIDGKFELGRLKYGDGKI
                     VLIDEISPDVLRVCRGFKPDKSGNCTVFNKCTVTNVADGKRTIKNKNQVVAAELIHAF
                     V"
     gene            complement(2967..4397)
                     /gene="gatB"
                     /locus_tag="PLG98_07865"
     CDS             complement(2967..4397)
                     /gene="gatB"
                     /locus_tag="PLG98_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOE33209.1"
                     /translation="MEFEAVIGLEIHVELNCATKLFCDCPNNPGDEPNANTCPTCLWF
                     PGAVPRLSRAALEKAALLCLGLNAELQPESAFDQKVYYYPDLPKGYQLSQAHLPLSRG
                     GWIDIVDEDGSTKRLRIHHIHMEEDVAKLVHEMEGRLPISLVDFNRAGAPLVEIVTEP
                     DFRTPHHAMEFLKALRTQVRYVGSSECSMENGTMRVDANISVRPKGTNQMNTKVEVKN
                     MNSIRHVGDAIAYEINRQSAAVGAGEAVVLHTRLWNPDKKATLPMRAKFEGPCVPDPT
                     VPHIILSAEDIASLRAKLPEMPAARAERFVNQYGFSVEEATYLSSDPEVAGYCEAVMK
                     EGVAPRTVMHWLTTQLMPAAKDRGQELNQTKITPARFAALLNMLSRDEINANAARDVL
                     AQLFDSDESPEAIVSARGFKQVSDSGALDAMIDKVLGEQAAAVADFKSGQGKAMGFLI
                     GQVMQASGGKANPKIIRELLAEKLNQ"
     gene            complement(4397..5593)
                     /locus_tag="PLG98_07870"
     CDS             complement(4397..5593)
                     /locus_tag="PLG98_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOE33210.1"
                     /translation="MDSIFFYQNPEQSAGSGKGALQKLKVTVQPNIFVKGWPTEAGSA
                     ALAGFKALEDAAVVERLSGAGAVLIGSTAMSEFGFGLKGSQAGCALGKKSADVELMLD
                     MAGEARLAAAREGVCGFKPSYGLISRIGLVGLIPSMETCGILARDIKSVRDILETIAG
                     PDERDFSLPDEKMPDFSAQDIEPAKTTLGIIPEVVNGLPSDQREAFAKICDALKKDGF
                     QIKELSFPDFSHFLLVHQIVGSVEASSCAGRYDSVRYGQRVPGAKNWNEMYLQSRGAA
                     FGTLLKSYLFQGAFFQFEKYDAYDDACRLRARLVKEMQTVAGQADALILPTVNRAASD
                     KTGSLADLYAQFAYTAFANVTGRPALHIPGANGAGVQLSAKRLDDGRLLSLGQYLLSH
                     RQGGKS"
     gene            complement(5608..7728)
                     /gene="aspS"
                     /locus_tag="PLG98_07875"
     CDS             complement(5608..7728)
                     /gene="aspS"
                     /locus_tag="PLG98_07875"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOE33211.1"
                     /translation="MNYSQRVFCGELSQEDVGRTVQLCGWVDAYRDHGGLLFIHLRDR
                     SGIVQIVFSPDVTPANVCQTAATLRPEYCITVTGMARKRMEGTENPNIETGKLEVFVD
                     GMTILSESDPLPFVITDKAMVAGGETAGAENVSEDLRMQYRYLDIRRPAMQNNLLLRH
                     RIFQGVRQFLDSRGFVEVETPVLTASTPEGARDYLVPSRVHPQNFYALPQSPQLFKQL
                     LMIGGLERYFQLARCFRDEDLRPNRQPEFTQLDIEASFIDEEFLYELIEELTVKMFAV
                     GGIELKRPFARMSYAEAMDTTGSDRPDLRFGLRFVDVTDAFKSTNYSIFRQILQRGGC
                     IKGINIKGQSEKLSKNILQNEYAKDIAPSFGAKGMTWMRAENGKLESNIVQFFSEAEL
                     DELKRRFDVHDGDVLIMVADPSYSIVTSALGQLRLHLADRLGLIPENSFYPVWITEFP
                     LFEPTDEGGVTSSHHPFTAPDRTDFDPKNIEELLSLKSRAYDLVMNGEELGGGSIRIN
                     NRDLQRKIFAALGLTEEDMKEKFGFFLRAFDFGAPPHGGLALGMDRTVSMILQTASIR
                     EVIAFPKNRSAACPLTGAPSAVKREQLAELGLLNLGEKDVLPGEAHKENLLDRVSWVS
                     RLGIAEAERPVMEEILAQSETLAAQVHDLAGAEEPIRSVAPAAGKTRPGAEAIRSPLA
                     ETGQLLKSAPAVKGNYFKVANIIE"
     gene            complement(7961..8608)
                     /locus_tag="PLG98_07880"
     CDS             complement(7961..8608)
                     /locus_tag="PLG98_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOE33212.1"
                     /translation="MKKILSVIVGFMMLMMIPGISIGSDAAPAKKAKAAKFKAAAVNK
                     PYVSVQGGVAFLTDSDVDAGRRSTAIEFDPGYALGVAAGYKMGKYRVEGEIGYQKNEV
                     DKALSGRRTSGDMKAYSFLVNGYYDIATKTRFTPYLTAGIGVAKVDGDFNRIAHVDDT
                     AFAYQVGAGVAYAVNRALAVDLKYRYMDTVDDFGFGNTNPEFSTHNVFLGLRYCF"
     gene            complement(8900..10120)
                     /locus_tag="PLG98_07885"
     CDS             complement(8900..10120)
                     /locus_tag="PLG98_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE33213.1"
                     /translation="MKTSGKTALLFTLYLSQGLPFGFQATALPVLLRDKGISLTTIGF
                     AGALALPWFFKPLWAPLIDRFWSARIGRRRSWIIPLQTMMVLTMFSSVFIAASGSIPL
                     LLLCIFLMNLCAATQDIAVDGLAVDILQRDELGPGNAAQVAGYKAGMVLGGGILVWLS
                     ARSGWESLFLGMAALAVLPLIVILCHREVEPDGSIVSKPPKFSDFISAMRNALQSPKA
                     GYFLLFIATYKIGESMIDMMFKPFLVDNGFTVQQIGLWVGTWGMASSLIGSVAGGWLA
                     ARISIVRALFLAASLRLIPLIGELWLSTFVPTAAQVVSVTVAEHFFGGMLTTALFAFM
                     MSRVDKKIGATHYTVLAAIEVFGKFPGTVFSGCLAENIGYTGLFSFGVVLSLLVLLLY
                     FPLRESTSPPKMNI"
     gene            complement(10199..11881)
                     /locus_tag="PLG98_07890"
     CDS             complement(10199..11881)
                     /locus_tag="PLG98_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOE33214.1"
                     /translation="MTDETASTPTDFIRDIINDDLKTNKNNGKVATRFPPEPNGYIHI
                     GHAKSVCLNFGIARDYKGTCNLRLDDTDPAGESMEYVQSIINDIRWLGFDWQDRLFYA
                     SDYFEKLYQFAVELIKSGNAYVCSLNPDEVREQRGTFTEPGKESPYRNRSVEENLDLF
                     QRMRAGEFADGAHTLRAKIDMASPNIVMRDPVIYRIKREPHYRTGTQWVIYPMYDFAH
                     CLSDALEGIVYSICTLEFENNRPLYDWIVEKLITGLRPRQIEFARLNLSYTVLSKRRL
                     IELVEKKYVRGWDDPRMPTVSGMRRRGYTAEAIRNFCAGIGVAKNDNLIDVALLEHCV
                     REDLNEHAPRAMCILRPLRVVIDNYPDNQVEQIACANHPQKPDMGTRNVPFSKVLYIE
                     RDDFMEDPPKKYHRLGPGREVRLRNAYVIKFESMMKDEKTGDVTELHCSYDPATLNGP
                     PADGRKVPGVIHWVSAGHAVPAEVRLYDRLFSIENPGSEEDFLKYLNPDSLETLTTCR
                     AEQNLKNAAAGSRFQFERLGYFCVDAKDSTSERLVFNRVVPLRDSWAKIAGK"
     gene            complement(12031..12639)
                     /locus_tag="PLG98_07895"
     CDS             complement(12031..12639)
                     /locus_tag="PLG98_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33215.1"
                     /translation="MKGYFKIISMFVGMCVLLCASWADAGVYYSTVQQPPAVSEKIKA
                     GTDFSVLPIDYSAINPKDLGYTNNAEWDADKSAVPKAFADAFPVLLKEANITNKKISV
                     VSGGDKITQGVIAQVAVTKINLNWNAWTNRPDEYVCKITFTDAADGQKLFSGEVNVST
                     FSGNPFTSAWGSFSGRMQSAAYNMAWVLTKIMTQGRIEPAEH"
     assembly_gap    12732..12831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13033..13854
                     /locus_tag="PLG98_07900"
     CDS             13033..13854
                     /locus_tag="PLG98_07900"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOE33216.1"
                     /translation="MNEKTGKASPPGFHGRPPRQDRQLSPVPAAHLRLSAHFRIVIFI
                     ATLCLSFCALNCRADVEQNDDVLRKKMVEEQLKARGIKNPQVLQAMSKVERHQFVPDA
                     LRKMAYADSALPIGEGQTISQPYIVAWMTEVINPQADMKVLEIGTGSGYQAAILAELC
                     HSVYTIEIIETLAKRAEKILGKHYQNVHVRTGDGYQGWPEAAPFDAILVTCAPTKVPA
                     PLAKQLKEGGRMVIPVGNVKAQELYVLVKRNGRLEQQAIIPVLFVPMLDKEGEKY"
     gene            complement(13955..14410)
                     /locus_tag="PLG98_07905"
     CDS             complement(13955..14410)
                     /locus_tag="PLG98_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOE33217.1"
                     /translation="MTKQETINSAVSFFNEGYACSQSVLLAFAGRFGLDEKQAKRIAS
                     TFGGGMGRLREKCGAVTGGFMVLGLAYGNADPNDMETKLTAYRKVRGLNRKIEALHGS
                     SCCSELLKKHATTADVTERKHHQIICQKIVGGVAGILYDMLEEEPPRAR"
     gene            14702..15352
                     /locus_tag="PLG98_07910"
     CDS             14702..15352
                     /locus_tag="PLG98_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOE33218.1"
                     /translation="MNVKTQELFNFYSHLAGMIAAVIGTVYLAIEASFSASGLVTALI
                     YGISVVFLFSASSLYHAFKKKENELSFWRRMDRLAIFFMIAGTFTPVCYFCLEGNQRW
                     AMIAFQWSLVGVGVISQIFFPRAPRTLYAMLYLFMGWSGLFIIKQVLASMSISQTVLL
                     FTGAGAFTAGGIIYAIKKPKLIPGIFSFHELFHILVLIGGALHYAMIYGVYAQLGA"
     gene            15364..16278
                     /locus_tag="PLG98_07915"
     CDS             15364..16278
                     /locus_tag="PLG98_07915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE33219.1"
                     /translation="MQCTPPDTAAACLLAAEADLFLKEYQQHYSAGETKPQQIGMPFF
                     LHAPGSDTGVLLIHGLMAAPEEVREWAEFLHQKGLTVYAPRLAGHGTSCKDLSTRRYE
                     DWLDSAERGHAILKKYCDKILVAGFSTGAGLALQSVILKPHDFEAVIAVSAPLKFKSF
                     SSRFAEVLNAFNRFCHSRGVNTLARDYIKNDADNPHINYLLCPVSAFVQVKKLMKKVN
                     RSLPDIRIPALVIQAKNDPKVAPQSGPEIFRRLGTNKKRFAWIDHDLHGIVRGNISGM
                     VFKEVELFLAEQNLIHIRPEMSNRLPGA"
     gene            16562..16762
                     /locus_tag="PLG98_07920"
     CDS             16562..16762
                     /locus_tag="PLG98_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOE33220.1"
                     /translation="MSEGKVKWFNESKGFGFIEKEEGGDVFVHFSSIQGEGFKTLNEG
                     QRVSFDVAQGKKGQEATNVRAI"
     gene            17033..>17524
                     /locus_tag="PLG98_07925"
     CDS             17033..>17524
                     /locus_tag="PLG98_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE33221.1"
                     /translation="MERDLTVIGIIPQYPKHSQQNIYARIKMPPVGIISVMSQIVRHP
                     RIRNIYAIDENNYRGPEDLNGLPDHFFLQQQQPAHLALLYGGMSNSIPRMFAIARQYK
                     SFGAVTITGGSHADALPQEALQSGIDIVVHGEGEETMAELIDVLSSESGVDLNRSRLS
                     QIKG"
BASE COUNT         4168 a         4611 c         4384 g         4261 t
ORIGIN      
        1 gccatcttcg taaatcagca gttgaccttc gggagatgtt ggaatcagat catttgccat
       61 aaccgatctc cttcagattc tgccagattt gtttgtcaag tattgcgcct tcttccatct
      121 gctttttgag ttctgctgtc agacgggaca tcttgtcttc aaagggttca ccatcgtctt
      181 caacttcagc cgcgccgacg tagcggccgg gtgtgagaat gtaattatgg ccgcggatgt
      241 catcaagctt ggctgatttg cagaagccgg ggatgtcttg atacctctct ggattccggc
      301 ctccgccgga atgacgcgat ttggcggtat cgccgcctgc ctgcgccgac gcttcggcag
      361 gcaggccgcg ccaggcgtga tatgtttctg caattttgag gatgtcctcg tcggtcagtt
      421 cacgatgcac gcggtcaatc agtgtgccca ttttacaagc atcaataaat aaagtttctc
      481 cacgtcggtc acgaaaacgt ccgttctttt tgtcgcgcga gataaaccac aggcagaccg
      541 gaatctgcgt tgaatagaat aattgtccgg gcagagccac catgcaatcc acgatatcgg
      601 cctcgatgat gctcttgcgg atttctcctt cgcccgactg attggatgac atggagccgt
      661 tggcaagaac aaagccggca atgccattcg gcgcgagatg ataaataaag tgctggaccc
      721 aggcgtagtt ggcattcgat gccggcgggg tgccgtattt ccagcgcttg tcgtctttga
      781 gcagttcacc gcgccagtcg ctgtcgttga acggaggatt ggccagaaca aagtccgctt
      841 tgaggtcggg atgcaggtcg cggtggaaac tgtcggcgtg ttctttgccg aggttggaat
      901 ctatgccgcg gatggcaagg ttcatcttgg ccagccgcca tgttgtgtga ttggattcct
      961 gtccgtagat ggcgatgtca ccgacacgtc cgccgtgttc ttcgatgaat ttttccgact
     1021 gaacaaacat accgccggaa ccgcaacagg ggtcatagac gcggccttta taaggggcga
     1081 gcatttcgac aagaatgcga acgacgcaac gcggcgtgta gaactggccg cctttcttgc
     1141 cttcggcgct ggcaaactgc gaaaggaaat attcatacac acggccaagt atatctttag
     1201 aacggttttc tctatcgccc aagccgatgt tcccgatgag gtcaatgagc tggccgaggc
     1261 gctgtttgtc gagacgcggg tgcgcgtagt ctttaggaag aacgccttta agagaagaat
     1321 tgtcccgttc gatggcgagc atggcgtcat caactgtctt gccgatggta ggctgcttgg
     1381 cgcttccttt aagatgtgac caacgggctt ctttgggcac ccagaagata ttgacggcgc
     1441 gatactcgtc cgggtcttcc gggtcggcac cctgcgtttt ttctgcttca agttttgcgt
     1501 gctgttcttc gaaagcgtcc gaaatgtatt tgagaaagat aaggccaagc acgacgtgct
     1561 tgtactcggc agcatccata ttggatcgca acgcgtcggc tgcccgccag agatctgctt
     1621 caaagccaag attcgcaccg ccgttattct tgtctttcgc catttcagcg ctcctgtcta
     1681 aaatattttc attagttttg ttcagatgtg tcttttgtag ccgaaaggca taatacaact
     1741 tcttgataac attgcaagaa taaaattgaa tcgattcaag gggttcacgc atccctcgaa
     1801 agagggatgg aaaataagtt caaggtttaa agggttcata gttcaaggtt aaaaaaccag
     1861 gctgtaaaaa tatttagtcc ttcaatttca gccgtttaag cgttcccgga aaaacggatg
     1921 aaaagcaatt ttttgtctgg attttaggat aaaagtattg tatcgatttg gccattgtta
     1981 ccttccgtga caatagtttc aaaaatcaat tttcaaatac aggctttcag gagagaaaaa
     2041 atggcggaga aaaaccttgt ctatcgcggc aaatcaaaag acgtttataa aatcaccggc
     2101 gggaaataca aaggaaagta ccgattcgtc tttacggacc ggggaacagg ctatatcgag
     2161 aaaggcaagg tggtcttcga tccgggctat gatgtggtgg tcggtgaaat tcccggcaaa
     2221 ggcgccattg cctgccgttt cgccacctat tttttcaaac tgctgaaggc aaagggcgtt
     2281 gccacccact acattgaaac catcagcggc aacgaaatga ttgtggaacc ggccatcccg
     2341 ctgagcatga aggaagccgc gccacaattt gccggatccg ccccgctgac gaatctggaa
     2401 ttcacctggc gcaataacgc gactggaagt ttctggcgtc ggtatccgtt tgtaaaaccg
     2461 tgcgtaaatc ttcacaaggt tgtcgaggcc tggaccaaag gcgacagcga tattctgatt
     2521 accatggaag cgctggagga aaccggcgcg ctgacgaaaa aagaaatcac cgacagcgtg
     2581 aagctggtga aaaaaatcgc cgaaatcgtt tccaacgaat ttacgtctaa aaagctgcac
     2641 gtcattgacg gcaaattcga actgggacgg ctgaaatacg gcgacggaaa gattgtgctg
     2701 atcgatgaga tatcgcccga cgtgctgcgc gtatgccgcg gcttcaaacc ggacaagtcc
     2761 ggaaactgca cggtgttcaa caaatgcacc gtcacgaatg ttgcggacgg gaaaagaacg
     2821 atcaaaaaca aaaatcaggt tgtcgcggct gagcttattc acgcctttgt gtaaaagcca
     2881 gacagcctgc aataatccca ggcaacagaa aaaggctctc tgaaggagtt tcccttcatt
     2941 gagcctttgt ttttgttaat cgttttttac tgattcagtt tctccgcaag caattcgcgg
     3001 atgattttcg gattggcttt gccgccggag gcctgcatca cctggccgat taaaaagccc
     3061 atggccttgc cctgcccgct tttaaaatcc gctacagccg ccgcctgttc gcccaatacc
     3121 ttgtcgatca tcgcgtccag agcgccgctg tcggaaacct gcttgaaccc cctggcgctg
     3181 acgatagctt ccggcgactc atcgctgtcg aaaagctgcg caagaacatc gcgcgccgcg
     3241 ttggcattga tttcatccct ggaaagcatg ttcaacaacg cggcaaaccg tgcgggcgtt
     3301 atttttgttt gattgagttc ctggccgcgg tctttcgcgg cgggcattag ttgcgtggtc
     3361 agccagtgca tgaccgtgcg cggcgccacg ccttccttca tcaccgcctc gcaatatccc
     3421 gcaacttccg ggtcggaact taaatacgtc gcctcttcga cggaaaaacc gtactggttg
     3481 acaaatcgtt cggcacgcgc ggccggcatt tccggcagtt tggcccgcag ggacgcgatg
     3541 tcttctgcgg ataaaataat gtgcggaacc gtcggatcgg gaacgcaggg cccttcgaac
     3601 ttggcgcgca tcggcagcgt cgctttttta tccggattcc ataaacgggt gtgcagcacg
     3661 accgcctcgc cggctccgac cgccgcgctc tgacggttga tctcgtaagc aatcgcgtcg
     3721 cccacgtgcc ggatggagtt catgtttttg acttccactt tggtgttcat ctgattcgtg
     3781 cctttcggac ggacggaaat gttggcgtca acgcgcatcg tgccgttttc catgctgcac
     3841 tcggacgacc ccacgtaacg cacctgcgtg cgcagcgcct tgagaaattc catcgcgtga
     3901 tggggcgtgc ggaaatccgg ctcggtcaca atttccacca gcggcgcgcc cgcccggttg
     3961 aaatccacca gactgatcgg caggcggcct tccatttcgt gcaccagttt ggcgacatcc
     4021 tcctccatgt gaatgtgatg aatgcgcagc cgtttggtcg atccgtcttc atcgacaatg
     4081 tcgatccagc cgccgcgcga cagcggaaga tgcgcctgcg aaagctgata acccttgggc
     4141 agatcgggat aataataaac cttttggtca aacgcgcttt cgggctggag ttcggcgttg
     4201 agccccaggc acagaagcgc cgccttttcc agcgcggccc ggcttaaacg gggaacggcg
     4261 cccggaaacc acaggcaggt cggacatgtg tttgcgttgg gttcgtcgcc gggattgttg
     4321 ggacaatcgc agaacaattt ggttgcgcag ttgagttcga catgaatttc caatccgatg
     4381 accgcttcaa attccattat gacttacctc cctgacgatg gctcaacaga tactggccta
     4441 acgaaagcag acgcccgtca tcaagccgtt ttgcggacaa ctgcacaccc gcgccgtttg
     4501 cgccgggtat atgcagcgcg ggccggccgg tcacattggc aaacgcggta taggcaaact
     4561 gcgcgtacag atcggccagc gatccggttt tgtcggatgc cgcgcggttg acggtcggca
     4621 atatcagagc atccgcctgc ccggcaacgg tttgcatctc tttgaccaga cgcgcgcgaa
     4681 gcctgcaggc atcgtcgtag gcatcgtatt tttcaaactg gaaaaacgct ccctgaaaca
     4741 ggtagctttt caaaagcgtt ccgaaggccg caccgcgcga ttgcagatac atctcattcc
     4801 agtttttggc gcccggcacc cgctggccgt aacgcacgga atcgtagcgt cccgcgcagg
     4861 aagaggcttc caccgagccg acaatctgat gaaccagcag gaaatgcgag aaatcaggaa
     4921 aggataattc cttgatttga aatccatctt tttttagcgc gtcgcatatt ttggcaaagg
     4981 cttcgcgctg atctgaaggc aatccgttca caacttctgg aatgatgcct agcgtcgttt
     5041 ttgccggctc gatgtcttgc gctgaaaaat ccggcatctt ttcatcggga agagaaaaat
     5101 cgcgttcgtc cggaccggct atcgtttcaa gaatatcccg aacggatttt atatcacgag
     5161 ctaaaattcc gcaggtttcc atggagggaa tcaggccgac aagaccgatg cgcgaaatca
     5221 aaccatagct cggtttgaag ccgcaaacgc cttcgcgggc tgcggccaga cgcgcttctc
     5281 cggccatgtc cagcatcagt tccacatcgg cggatttttt ccccagcgca caaccggctt
     5341 ggcttccttt gagtccgaaa ccgaactcgc tcatggccgt ggaaccgatc agaacggcgc
     5401 ccgctccgga aagccgctcc accacagcgg cgtcttccag ggctttaaaa ccggccagcg
     5461 ctgccgatcc cgcttccgtc ggccagccct tcacaaagat attaggttga acagtcactt
     5521 tcagtttttg caaagcccct ttgcctgagc ctgccgactg ttcaggattt tgatagaaaa
     5581 aaatggaatc catcaggatg tctccctcta ttcgataata ttggcaactt tgaaataatt
     5641 tcccttgact gcaggcgcgc ttttcagcaa ttgtcccgtc tcggccagcg gcgaccgtat
     5701 ggcctcggct cccggacgtg ttttgcccgc cgcgggcgca accgaccgga tcggctcttc
     5761 agcgccggcc agatcgtgaa cctgcgcggc cagcgtttcg gactgcgcaa gaatttcctc
     5821 catcaccgga cgctccgcct cggcaatgcc cagacgcgac acccacgaga cgcgatccag
     5881 caggttttct ttatgagctt ctcccggcag cacatccttt tcacccagat tcaaaagtcc
     5941 cagctccgcc agttgctcgc gtttcaccgc ggagggcgcg ccggtcagcg gacaggccgc
     6001 gctgcggttt ttcggaaacg cgatgacttc gcggatcgac gccgtctgca gaatcatcga
     6061 cacggtacgg tccatgccca gggccagtcc gccgtgcggc ggcgcgccga agtcaaacgc
     6121 ccgcaggaaa aacccgaact tttccttcat gtcctcttcc gtcaatccca gcgccgcgaa
     6181 aatcttgcgc tggaggtcgc gattgttgat acggatactg ccgcccccca gttcctcgcc
     6241 gttcatgacc aggtcgtagg cgcgcgattt cagggataaa agttcttcga tgtttttcgg
     6301 atcgaaatcc gtgcggtccg gcgctgtaaa cggatggtgg ctggaagtca cccctccctc
     6361 gtccgtcggt tcaaacaacg gaaattcggt aatccagacc ggataaaaac tgttttccgg
     6421 aatcagtccc agacggtcgg ccagatgcag acgcaactgc cccagcgcgg aggtgacaat
     6481 gctgtaggag ggatcggcca ccatgatcaa cacatcgccg tcatgaacat cgaagcggcg
     6541 cttcaattcg tccagttccg cctcgctgaa aaactgcacg atattggatt ccagcttgcc
     6601 gttttcggcg cgcatccagg tcatgccctt ggcgccgaac gacggcgcga tgtctttggc
     6661 gtattcattc tgcaaaatat ttttgctgag tttttcggat tggcccttga tgttgatgcc
     6721 cttgatgcag ccgccgcgct gcagaatctg gcggaagatg gaataatttg tgctcttgaa
     6781 ggcgtccgtc acatccacaa aacgcaggcc gaaccgcaga tcgggacggt cggaaccggt
     6841 cgtgtccatc gcctcggcgt aactcatgcg cgcaaacgga cgttttaatt caattccgcc
     6901 gacggcaaac attttgaccg tcagctcttc aatcagttcg tagagaaatt cctcgtcgat
     6961 aaatgacgct tcgatatcga gttgcgtgaa ttccggctga cggttcggcc gcagatcttc
     7021 atcgcggaaa cagcgcgcca attgaaagta tcgctccagg ccgccgatca tcagcagttg
     7081 tttgaagagc tgcggcgact ggggcagcgc gtaaaaattc tgcggatgga cacggctggg
     7141 gacgagataa tcgcgcgccc cttcgggcgt gcttgcggtc agcacgggcg tttccacctc
     7201 cacaaacccg cgcgaatcga gaaactgccg cacgccctga aaaatacgat gcctcagcag
     7261 cagattattc tgcatcgccg gacggcggat atccagatac cggtactgca tgcgcaaatc
     7321 ctcggaaaca ttttccgcgc ccgcggtttc cccgcccgcc accatcgcct tgtcggtgat
     7381 gacaaacggc aacggatcgg attcggaaag aatcgtcatc ccgtcgacaa aaacttccag
     7441 ctttcccgtt tcgatgtttg gattttccgt gccttccatt ctttttctgg ccatgcccgt
     7501 taccgtaata cagtattcgg gacgcagcgt tgccgcagtc tgacagacat tggcgggtgt
     7561 gacatccgga ctgaaaacaa tctggacaat gccgctgcgg tcgcgcagat gaataaacag
     7621 caggccgccg tgatcgcggt aggcgtccac ccagccgcaa agctgaacgg tccggccgac
     7681 atcctcctgc gaaagttcgc cgcaaaaaac acgttgggaa taattcataa gacaagccct
     7741 ttatatgaaa ataaaaacgc aaaattgttc atagaacaat tttgtgccgc ggagcatacc
     7801 aaaactgtac cgatgtttca aggcattaaa cagacaatcc tccggtatcc ggatcaaaac
     7861 acagatattg ataattggga tttctacaga taactaaaaa acaaaagcca gaggtctttt
     7921 acagacctct ggcttcactg gatcgatttc tgaaaatcga ttagaagcag tatctcaagc
     7981 ccaggaatac attatgtgtt gagaattcgg gattggtgtt tccgaaacca aaatcatcaa
     8041 ccgtatccat gtaacgatac ttcaaatcaa cagcaagagc tctattgacg gcataagcaa
     8101 caccggcgcc gacctgatat gcgaaggcgg tgtcatcgac atgagcaatg cggttaaaat
     8161 cgccgtcgac ttttgcaaca ccgatacctg cggtcaggta aggtgtgaat ctggtcttgg
     8221 tggcaatgtc ataatagccg tttaccagga aggaataagc cttcatatcg cccgaggttc
     8281 tccggccgga aagagctttg tcaacttcat tcttctgata gccgatctcg ccttccactc
     8341 tgtatttgcc catcttgtaa ccggctgcca cgcccagtgc ataacccgga tcaaattcga
     8401 tcgccgtgct tctacggccc gcgtcaacat cgctgtccgt taaaaaggcc acgccgccct
     8461 gtacgctcac atacggtttg tttactgctg ctgctttaaa tttcgctgct ttggctttct
     8521 tggcgggtgc tgcatcgctg ccaattgaaa tcccgggtat catcatcaac atcataaatc
     8581 cgacaatcac tgataaaatc tttttcataa atcgtctcct tttaaaaaaa ttttttgact
     8641 taaccgtgtt tcacatttct tggaaactac aacaaaacaa cctcttcatt gcctatataa
     8701 ataaaaaatt tgactctaca tgtcaagcaa tttctgcccc gaacggttcg atttggaaaa
     8761 cggtttttcc tggaccggcg taaacatcct tgatcattga ttattttttt caatttataa
     8821 actatctgtc cgaagacggt aaaaatataa tttcaaaaac attttcggga tcatgactat
     8881 ttttttcatc aatttccttt tatatattca ttttcggcgg cgacgttgat tcccgcagcg
     8941 ggaaatagag cagcagcacc agaagcgaca gcaccactcc gaaagaaaaa agtcccgtat
     9001 aaccgatatt ttccgcaaga cagcccgaga aaacagtccc cggaaatttt ccaaaaactt
     9061 caatggccgc cagcacggta taatgcgtgg cgccgatttt cttgtcgaca cgcgacatca
     9121 tgaacgcgaa aagcgccgtg gtcagcatcc cgccaaaaaa atgctccgca acggtaacgc
     9181 tcaccacctg cgccgcggtg ggcacaaacg tggacagcca gagctcaccg attagaggga
     9241 tcaacctcaa tgacgccgcg aggaaaaggg cgcgtacaat ggaaatcctg gctgcaagcc
     9301 atccaccggc aaccgacccg atcagcgatg acgccattcc ccaggtgccg acccacaatc
     9361 cgatttgctg gacggtaaaa ccgttgtcta cgagaaaagg tttgaacatc atgtcgatca
     9421 tcgattcacc gatcttgtac gtggcgatga acaaaagaaa ataaccggcc ttgggagact
     9481 gcagggcgtt gcgcatcgcg ctgataaaat cggaaaattt cggcggcttg ctgacaatgc
     9541 ttccatccgg ttccacctcc cgatggcaca aaatgacaat cagcggcagg accgccagcg
     9601 ccgccatgcc cagaaacagg ctctcccatc cggaacgggc gctcaaccag accagtatgc
     9661 cgccgcccag aaccatgccc gccttgtaac cggcgacctg tgcggcattg ccgggaccga
     9721 gttcatcacg ttgcaaaatg tccaccgcca agccgtcaac ggcaatatcc tgtgtggcgg
     9781 cgcaaagatt catgagaaaa atacacaaca gcagcagagg aattgaaccg gatgcggcaa
     9841 tgaagacgga cgaaaacatc gtcaaaacca tcatggtctg caggggaata atccacgaac
     9901 ggcgccgtcc tattcttgcc gaccagaagc ggtcgatcaa cggcgcccag aggggtttga
     9961 aaaaccaggg cagggccagc gctccggcaa aaccgatcgt cgtgagcgaa atgcctttgt
    10021 cgcggagaag aaccggcaaa gccgtggcct gaaagccgaa aggaagaccc tgtgaaaggt
    10081 agagcgtaaa cagaagtgcc gtcttgccgc ttgtcttcat ggtgcggatc tctcgattca
    10141 gaaattacat cgctatctac ttcatgcggc aaaacgggct gttttcaaaa cgttttcatt
    10201 atttgccggc aatcttcgcc caggaatctc tcagcgggac aacccggttg aacacgagcc
    10261 tttctgatgt cgaatccttt gcatccacgc aaaaatatcc cagtctttca aactgaaacc
    10321 ggctgcccgc tgcggcattt ttcaggtttt gctctgcccg gcaggttgtc agcgtctcca
    10381 gcgaatccgg attcagatat ttcagaaaat cctcttcgct tccgggattt tcaatactga
    10441 acaacctgtc gtaaagccgg acttcggcgg gaacagcgtg tccggccgat acccagtgaa
    10501 tgacgcccgg aaccttgcgt ccatcggccg gcgggccgtt cagtgtcgcc ggatcatagc
    10561 tgcagtgcag ctcggtgacg tcgccggttt tttcgtcttt catcatgctt tcaaatttta
    10621 taacgtaggc gtttctcaaa cgcacttcgc gtccgggacc gagacggtga tattttttcg
    10681 gcggatcttc catgaagtcg tcgcgctcaa tgtagaggac tttggaaaag ggaacgtttc
    10741 gtgtgcccat gtccggtttt tgcggatgat tggcgcaggc gatctgttcc acttgattgt
    10801 ccggataatt gtcgatgaca acgcgcagcg ggcgcagaat acacatcgcc cggggagcgt
    10861 gctcgttcag gtcctctcgc acacagtgtt ccagaagcgc cacatcgatc agattatcgt
    10921 ttttggccac gccgatcccg gcgcaaaaat tgcggatggc ttccgccgta taaccgcggc
    10981 ggcgcattcc cgacacggtg ggcatgcgcg gatcgtccca cccccgaaca tactttttct
    11041 ccaccagttc gatcagcctt cgtttactca gtaccgtata actcaaattg aggcgggcaa
    11101 attcgatctg acgcggacgc agaccggtaa tgagtttctc cacaatccag tcatagagcg
    11161 gacggttgtt ttcaaattcc agggtgcaga tggagtaaac aatcccttcc agcgcatcgg
    11221 aaaggcaatg cgcaaagtcg tacatcggat aaatgaccca ttgcgtgccg gtgcggtaat
    11281 gcggttcgcg cttgatgcgg tagatcacgg gatcgcgcat cacgatattg ggcgaagcca
    11341 tatcgatttt ggcgcgcagg gtatgggcgc cgtcggcaaa ttcgccggcg cgcatccgtt
    11401 gaaacaaatc gagattttct tcaaccgagc ggtttcggta agggctttcc ttgcccggtt
    11461 ccgtgaacgt tccgcgttgt tctctgactt catcaggatt aagagaacag acataggcgt
    11521 tgccggattt gatgagttca accgcaaact gataaagttt ttcgaaataa tcggaggcgt
    11581 aaaacagccg gtcttgccag tcgaacccca gccatcggat atcgttgatg atcgactgaa
    11641 catattccat cgattctccc gccggatcgg tgtcgtccag ccgaagattg caggttccct
    11701 tataatccct ggcgatgccg aaattgaggc atacggattt ggcatgaccg atatgaatat
    11761 agccgttcgg ttccggcgga aaacgggtcg caactttccc gttgtttttg ttcgttttca
    11821 gatcgtcatt gatgatgtcg cggataaaat cggtcggggt tgaggctgtc tcgtctgtca
    11881 tgggaatatt caaactcctt cagttccata aaaaatacgc tctggaccta taatatttac
    11941 tgaaatatag caagagcgga gcatgcaaaa caacacacgc atccgccgtt gatgttttca
    12001 aaaatgctta ctttcagtcg aaaaatcgaa tcaatgttcg gccggctcta tcctgccctg
    12061 cgtcatgatt ttcgtcagaa cccaggccat gttgtaggct gcgctttgca ttcttccgct
    12121 gaaactgccc caggcgctgg taaaaggatt tccggaaaac gtgctgacgt tcacctcgcc
    12181 cgagaataat ttctgcccgt cggcggcatc ggtaaacgtt attttgcaga catattcatc
    12241 aggccggttc gtccaggcgt tccagttcag attaattttg gtcacggcca cttgcgcaat
    12301 cacaccctgg gtgatcttgt cgccaccgct caccacggat atttttttgt tggtgatatt
    12361 ggcttccttc aacagaaccg ggaaagcatc ggcaaatgcc ttgggaactg cacttttatc
    12421 ggcatcccat tccgcgttat ttgtatagcc cagatccttt ggattgatgg cgctgtaatc
    12481 gatgggcaga acgctgaaat ccgtgccggc cttgatcttt tcggaaaccg ccggcggctg
    12541 ttgcaccgtt gaataataaa caccggcatc cgcccatgat gcgcacagca ggacacacat
    12601 gccgacaaac attgagatga ttttaaaata acctttcatt ttctgcctcc tctgaaaatc
    12661 gtttaatgtt tcaataatgc atgaactttc tttcgaatgc atttttacca gttatacgtt
    12721 catttttcta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgcatgaa
    12841 ctttctttcg aatgcatttt taccagttat acgttcattt ttctatcctt ttttccattt
    12901 tttctaacgt ccccccggca gggatttttt actcattttt ttaaaaaagc tgatttgtaa
    12961 gccgggatat ggtaaaaacg agatacttat ccctgtttct gaaatgaaaa aatattaacg
    13021 gctggaacga tcatgaacga aaaaaccggc aaagcatctc cgcccggctt tcacggccgg
    13081 ccgccaaggc aagacaggca gctgtcccct gtaccggctg cgcatttacg tctgtccgca
    13141 catttcagaa tcgttatctt cattgccacg ttatgtttat ccttctgcgc gctgaactgc
    13201 cgggccgatg tggaacaaaa tgatgacgtc ttgagaaaaa aaatggttga ggagcagctc
    13261 aaagccagag gcattaaaaa tccgcaggtg ttgcaggcaa tgagcaaggt ggaacgtcac
    13321 caatttgtcc ctgatgccct gcgcaaaatg gcttacgccg attccgctct tcccatcggg
    13381 gaaggacaga ccatctctca gccatacatt gtcgcttgga tgacggaagt gatcaaccct
    13441 caagcggata tgaaggtttt agaaatcggc accggttccg gttatcaggc cgcgattctg
    13501 gccgaactct gccacagtgt ttataccatt gaaattattg aaacgctggc caaacgcgcc
    13561 gaaaagattc tcgggaaaca ttaccaaaat gtccatgtca ggaccggcga cggctatcag
    13621 ggatggccgg aggccgcgcc ttttgatgct attctggtca cctgcgcgcc caccaaagtg
    13681 cccgcgcctc ttgcgaaaca actcaaggaa ggcggcagaa tggtcattcc cgtgggaaat
    13741 gtcaaagcgc aggagttgta tgttttggtc aaacgcaatg gacgccttga acagcaggcg
    13801 atcatcccgg tccttttcgt gcccatgctg gacaaggagg gggaaaaata ctgatcgacg
    13861 tgatgagcat ctgtgccagg tattcaccgg catttccgtc tttgatgttt aaaacctgaa
    13921 ggctgacgaa tcccttggca tctgccggtc agacttatct tgcgcgcggc ggttcttctt
    13981 ccagcatgtc gtacaatatt ccggcgacac cgccgacaat tttttgacag atgatctgat
    14041 gatgctttct ttcggttacg tcggcggttg tagcatgttt tttcagcaac tcggaacaac
    14101 aactgctgcc gtgaagagct tcaatcttcc ggttcaaccc ccgcactttc cgataggcgg
    14161 tcagtttggt ttccatatcg ttgggatcgg cgttgccgta tgccagtccc agcaccatga
    14221 agccgccggt aacagcgccg catttttccc gcagtctgcc catgccgccg ccgaaggtgg
    14281 aggcaatccg cttcgcttgc ttttcatcca ggccgaaccg gccggcaaaa gccagcaaga
    14341 cggattgaga gcaggcgtat ccctcgttga aaaaagacac ggcgctgttg attgtttcct
    14401 gtttggtcat gaatgtattt tcggacagac gattggtttg tccccgcgtc agggatatcc
    14461 gaagaaatgc tttttgtcaa aaatttaatc ggaagcagga accccgtttg attccggcaa
    14521 tcaagcgccc ttcccgtccg ctgagagccg atatccatgc gactcctttt cctctgaaag
    14581 gcgtcgaatg cgaacggcca acaagaatcc ttagtgaagc gcaacattaa ttgcttgtgt
    14641 tttatgcaaa tctgtgaatg agattcaaca ggttaaaaat cgaatttcag gataggattt
    14701 tatgaatgtc aaaacccagg agttgtttaa tttttattca catttggccg gaatgatcgc
    14761 ggccgttatc ggcaccgtgt atctggccat cgaagccagt ttctcggcat ccggtctggt
    14821 cactgctctg atttacggga tttccgttgt ttttcttttt tcagccagtt cgctatacca
    14881 cgccttcaaa aagaaggaaa acgagctgtc cttctggcga agaatggatc gtctggcgat
    14941 ctttttcatg attgcgggca catttactcc ggtctgctat ttttgcctgg agggcaacca
    15001 acgatgggcg atgattgctt ttcagtggag ccttgtcggg gttggcgtaa tctctcagat
    15061 ttttttcccg cgcgcgccgc ggacacttta cgcgatgctc tatttattca tgggatggtc
    15121 cggactcttc attattaaac aggtcctggc cagtatgtct atttcccaga cggttctgct
    15181 gtttaccggc gccggcgcct ttacggcagg cggcattatt tatgccatca aaaaaccgaa
    15241 attgattccg ggcattttca gttttcatga acttttccac atcctggttc tgattggtgg
    15301 agctctgcat tacgccatga tttacggcgt ttatgcgcag ctcggtgcct gagcgataca
    15361 ctaatgcaat gtacaccccc tgacacggcc gcggcctgcc ttctggcagc cgaagcggat
    15421 ttattcctca aagagtatca acagcattat tctgccggcg aaacaaagcc tcaacagatc
    15481 ggcatgccgt tttttctgca cgcgcccgga tccgataccg gcgtgcttct gattcacggc
    15541 ctgatggccg cgccggaaga agtcagggag tgggcggagt ttctgcacca aaaaggtctg
    15601 accgtttatg caccgaggct cgccggccat ggaacatctt gcaaagatct ttcgacacgc
    15661 cgctatgaag actggctgga ttcggcggag cgcggccatg ccattttgaa aaaatactgc
    15721 gacaaaattc tggtggccgg tttttccacc ggggcgggcc ttgcactgca atccgtcatt
    15781 ttaaaacccc atgattttga agccgtgatc gccgtcagcg caccgctgaa attcaaaagc
    15841 ttttcgtcga ggtttgcgga agttctcaac gcgttcaacc gattctgcca ttcccggggc
    15901 gttaacaccc ttgccagaga ctacatcaaa aacgacgcgg ataatcccca catcaactat
    15961 ctgctctgtc cggtaagcgc atttgttcag gtgaaaaagc tgatgaaaaa agtcaaccgg
    16021 tcgcttccgg acattcgcat tccggcactg gtgattcagg cgaaaaacga tccgaaagtc
    16081 gcgccgcaaa gcggtcctga aatattcaga cgtctgggca caaacaaaaa acgttttgcc
    16141 tggatcgatc atgatctgca cggcattgtt cgcggcaaca tttccggtat ggtctttaag
    16201 gaagtggaat tattcctcgc ggagcagaat ctcattcaca tccggcctga aatgagcaat
    16261 cgtttgcccg gagcataaac gctgtaacgt cctgcattat atgatgaaat cgactcttcc
    16321 cgcacactat aacttgagaa aagaatttgt tttgtaaata aagcttgact attcctccgc
    16381 gataccgtaa ggtcgcgcca acttcagatc cacaagattg agatttgaag gaaagagttg
    16441 aatttagaca ttttgaagca cataggttag ccgccgggta ctttagagtc ttggtggctt
    16501 ttttagttct gtcatcgtgc tgattcgact tttgaaaatt tatgaaagga ggatgagaac
    16561 tatgtcagaa ggtaaagtca aatggtttaa cgaaagcaaa ggcttcggct tcatcgaaaa
    16621 ggaagaaggc ggcgatgtat tcgtacattt cagctcgatt cagggtgaag gtttcaagac
    16681 cctaaatgaa ggccagcgcg taagttttga cgttgcgcag ggcaaaaaag gtcaggaagc
    16741 cacgaacgtc agagccatct aatgagcgac gcttccccct gccaggcgca caacgccctg
    16801 gtagaaacca gataaataga gcatcccgtg ttgtttgaaa aatcagccgg ggtgcttttt
    16861 ttattgatgg tttgcggcaa ggcatccttc ttgctttagc ttgtccatcg ccaatattga
    16921 ctttttgcgg tgaatggatt atttttatca tccaaagagc cgtattttcc agcccaacgt
    16981 ttattttcga accgtccttt tctgatgaaa gatttacagg gagggtttca tcatggaaag
    17041 agatttaacc gtgatcggca tcattccgca gtacccgaaa cattcccaac agaatattta
    17101 cgcccggatt aaaatgccgc ccgtcgggat catatccgtc atgagtcaga tcgtccgtca
    17161 tccccgcatc aggaatatct acgcgatcga tgagaataat taccgcgggc cggaggattt
    17221 gaatggtctg ccggatcatt tctttttaca gcagcaacaa ccggcacacc tggccctgtt
    17281 gtacggcggc atgagtaatt cgatcccccg catgttcgcc atcgcccggc aatacaagag
    17341 cttcggcgcc gtgacgatta ccggcggcag ccatgcggat gctcttccgc aggaagcgct
    17401 gcaatccggc attgacattg tggtccacgg cgaaggggaa gaaaccatgg cggagttgat
    17461 cgatgttctg agctccgaaa gcggtgtcga tctgaaccgt tccagattgt ctcagatcaa
    17521 agga
//
LOCUS       DAOSME010000048        16610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4150_length_16610_cov_22.503473, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000048 DAOSME010000000
VERSION     DAOSME010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16610
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4150_length_16610_cov_22.503473"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLG98_07930"
     CDS             complement(<1..804)
                     /locus_tag="PLG98_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE33222.1"
                     /translation="MAQKKDPLYLTWMGTAGVLIEDDHDGILIDPYISRPGLLSVVLG
                     LSLKPDRDAIEKWLTRPGTKNIRAVLVSHSHFDHCLDAPFFALKTNALFIGSKSALNA
                     ARGVPLDESGLKEAGIGRDIKIGAFTLRFIESAHGPAFLGRVPYAGTIDRPLTGRHSA
                     RDYKLGQTYSILITHASGTILHHGSAGFINGMYDGITSDIVLLGIGGRGHTQTYLENV
                     PLKLNAKIVIPIHFDNFFVSLDRKMKNLPGVNFQEFLTTAGKYGNRFQLK"
     gene            complement(853..1617)
                     /locus_tag="PLG98_07935"
     CDS             complement(853..1617)
                     /locus_tag="PLG98_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE33223.1"
                     /translation="MTNQQAHQKISTALMRDIFFHKLQPGEKLPSERELSGSLNVDRT
                     SLRVALKQLEAMGLLDIRRGDGIYVKDFLKHAGIDFFRTLFSRIETLEDELAADLFVI
                     DEVWEWWIIMFPEMLKLAAQRASARDLKALSDLMDSELNNLDHRDVLIELWLDEQDMI
                     ARAVNNMVLILIFNSSRPLRRSMLRIYHDAVNRENMEQFIISKKSLLMDFLMNSDKTM
                     LADVEQYRDILIAQRQVARSRLIQSGPSPGKTGKEK"
     gene            1915..3318
                     /locus_tag="PLG98_07940"
     CDS             1915..3318
                     /locus_tag="PLG98_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOE33224.1"
                     /translation="MRDVYVIGAHTIKFGKYIEKSIKDLAAQTVISCLKEAHLEKKDI
                     QALWFANSGWGYSKGQDCIRAQVALRPIGIDAIPMTNVENACAGGSTAFHHAWLGIAG
                     GLYDITMAVGAEKLYDNNKMKVFAGFLSGLDIENLSSIIANISFYGMSEQDKKDMQAH
                     VAKYAALAKKNAAIKRKKKGVGSRFSDLLDMFKVYLRLSDAVGSETVRKMRKLFSGDH
                     SPFMDVYGFAARQHMKKYGSTIEQLALIASKNHFHSSLNPNAQYTFTITPEEVLADRM
                     VSWPLTRSMCAPIGDGAASAVLCDQATVKKLGLSSQAVKIRASVLGSGRERSFDDTDI
                     GERLSKIAYEKSGLGPGDMDFAEVHDASAFGELHQAEALGFCGEGEGGRLAESGATRL
                     GGKIPVNVSGGLESRGHPVGASGLAQIHELVTQLCGKAGKRQVEGAKIAIAENGGGAL
                     GQEEAAMCIHILEGPSK"
     gene            3372..>4138
                     /locus_tag="PLG98_07945"
     CDS             3372..>4138
                     /locus_tag="PLG98_07945"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE33225.1"
                     /translation="MDLNKKFPEKRVVITGAGSGLGRALALEFARRNWRIAIAEINDE
                     RAAESAKMVREQGGIPLTIHCDVTLPRDMENVLTIVRREWKGVDILINNAGVASAGWF
                     ERIPLEKWDWIIGINLMSIIYGCRTFIRYFKESGTGGYIVNVASSAGIASLPEMVSYN
                     TTKAAVISLSETLRIELSKINVGVSVVCPTFFKTNLMDQFSSPDERQRQLADTFFGNA
                     KITSEQVARHTVASIEKNRFYVITQADGRNMWRMKRW"
     assembly_gap    4139..4238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4359..6041
                     /locus_tag="PLG98_07950"
     CDS             4359..6041
                     /locus_tag="PLG98_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE33226.1"
                     /translation="MGIAGDIVIIVMAALLGGLIAQMLKQPLILGYILAGVVVGPYTG
                     GVTISDIHSIEKLAEIGVALLLFALGLEFSLRELRPVRAIALFGTPVQIILTTLYGFG
                     IGRILGWSWTSSVWFGALISLSSTMVLIKTLEKQGMMGTLSSRVMIGVLIVQDLAVVP
                     MLIILPQLNNVQDGMAILLVAAVKAAIFLTVMILVGTKIIPRLMRYIANWNSRELFLI
                     TTTTIGLGIGYATYIFGLSFAFGAFVAGMLLSQSDYGYQALSDIIPLRDVFSLMFFTT
                     IGMLLDIRFLLANWEIILLLVGLVMLGKAVILAVLSRVFGYHNVIPLAMGLGLSQVGE
                     FSFILGRVGISTKSITGEFYTMVLTMTIVTMLLTPFISGLTTPLYAMARRWMKPLKLE
                     PLNFPSKGLKDHIVIAGGGRVGMYVANVLQQKNIPFVILEYNSRRVHEVRKSGFPIIF
                     GDAAKTIILEAADLKRAKLLLITTPVAVISLGIVAHAQRINPGIRIIARAEGEEQMKA
                     LYTKGVTYVVQPEFEASLEIVNQTLLNLGIPAEQFKTFTEDARQELHRPLSV"
     gene            6089..7942
                     /locus_tag="PLG98_07955"
     CDS             6089..7942
                     /locus_tag="PLG98_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE33227.1"
                     /translation="MKLNLKTIIRKCLGFVNFRSMTKATLWWMWPRRHRVFFIFEGRE
                     FTYGDVYRQAQRYAHFFLSIRKKLVDSGKLASDQRMAFGVYMENTPEFLFASFGAALS
                     NSVLFAINTGFRGDILGKVMEQAGISFLIVNDSTLKEVEGVVKNVNIIGPENIYVVRE
                     GEKSLPDGFQWLDKAVEEAQQIDVPNRYRVRVDNFSPVIVIYTSGTSGMPKGVPCTHI
                     KMFGAGFVVQSAVHLNRRDRGYISMPLFHSNAWYIGILPQMIAGGSFVLKRRFSASAF
                     EDDMLAHGVTFLNYVGQPLHYIIDALERKYGSGEAVEAALAKHPRNRFRKAYGNGATV
                     NDRIKLKRYLGMDHIYEIYGSTEAVITTANCPGDPVDSVGRVDGSVRILDEEGKECEP
                     GMVDEKGALLNYDKAVGEICRKVGDNNLRFDGYYGNAGATSQKFRNGWYHSGDLGHIR
                     IIKGKRYMFFNGRTDDWIRKDGENFSAENVLDFAQKIPCVDCAIAYGAPCEVADEKVM
                     ITVQLKEGADFNPDEVHEWFIQQQKEGGMDPKWMPDYIRVIEKFSVTDTQKILVRPFK
                     RENFNIEINASMRIYFRRRGDTAYRLLTPDEFRKIREDFKTNGREAILASC"
     gene            8010..8648
                     /locus_tag="PLG98_07960"
     CDS             8010..8648
                     /locus_tag="PLG98_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOE33228.1"
                     /translation="MLQKIIIATIIFLFSLSLCWAAEGIKLPAPQMDGGKNLMNAFKE
                     RKSERSYSAKKIPLNVLSNMLWAACGINRPEAGKRTAPSAMNRQQIDVYVATADGLFL
                     YEAKGHLLKPILNQDIRPLTGKQSFVAQTPVTLIYVADYKKMGNISNEEKAFYAATDT
                     GFISENVYLFCASEGLGTVVRGSIDKEALAKTMKLGDHQKIVLAQSVGYPKE"
     gene            complement(8820..9080)
                     /locus_tag="PLG98_07965"
     CDS             complement(8820..9080)
                     /locus_tag="PLG98_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HOE33229.1"
                     /translation="MEETENKLHLEYPCAWIYKIIGTDEDEMRMAIDEIIQDRNCLVT
                     FSRSSRNNSYRCLNVEVIVESESHRQTLYELLKAHRAIKIIL"
     gene            9610..11526
                     /locus_tag="PLG98_07970"
     CDS             9610..11526
                     /locus_tag="PLG98_07970"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOE33230.1"
                     /translation="MKSFLANHYAIKDLNAEINNQVVDQMIKRLDPSKTLLYDSDVQN
                     LRPVLRRAFSSMQSGDCALLKPVYDLLVTRARENEKIIRRILGSNYRIDEDAELYIDV
                     KKRPYVKTAAEKYDLLKKVVQFQIENDLLSGVTLAEAKKQQVHRYELQTARVLERRPE
                     KLIEYAAEAFAQALDPHTNYLSPDNLEDLKIHMQLSLEGIGALLSSDNGFTVIEELIP
                     GGSAEKSGLLKPKDKIIAVAQKRAKPVNVIDMDLRDVVKMIRGKKGTQVTLMILRQSE
                     RTYRFDITLTRDKVYIKDQEAKVDYQTRMVNGKKYSFAVIDLPSFYGDEKENKSSYED
                     VRALLMEARQRGVDGVVLDLSRNGGGLIGEAVQIAGLFLGRGPVVATKDNSEKLTVFV
                     NGAATSEMKLRKNKIITFPAEDRPLYTGPLVVLTSRLSASASEIVAGALQDYRRAIIV
                     GSDRTFGKGSVQTLAPLPRDLGGMKVTTGLYFLPGGKSTQKTGVPVDVRLPGFFVLED
                     VGEAELEYPLPAQAIQPFIKASSNSALLWTTVDHPMVAKLSIKSQARIAKDPKFKEII
                     QKNKEAAEKKGLIRLVDLREERKKEGGSNKKETPAELKQKVRDQYAPYVNESINILSD
                     MMTAKAALPMAKAL"
     gene            complement(11731..12873)
                     /locus_tag="PLG98_07975"
     CDS             complement(11731..12873)
                     /locus_tag="PLG98_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33231.1"
                     /translation="MTDKDKKNALEFFPPTLIREARREDDEAICALMKRTFMPGRIPL
                     TMDCQPSFFSAIDVEGYVHRTAVAENNHRIVGVVSMSKRRLYLNGVPEDFGYIGGLRI
                     DSSLRNTTILVRLHNILKKWHQEGFGVRFYLSAIPYDNVGARNIMTSGRAGFPDCTDM
                     GVLYNAAIPLFRRRLPRPQKGLRIVRGTAVGADAIAEFLNRNGSRRQFFPVYTADDIT
                     ADNGILRGLHPDDFYVAVERNKISGVMACWDQLPFRRMLIADYPWHLHWFKRLSAPLA
                     GILHTAPMPEPGEPLRNIVAACIAIENNHPKIFNLLLQTILHDHYDTGKAFLVVGLME
                     KDPLISALKSRLHISTRTCIYMMSGNGYNILSEMDERPPYLELGAL"
     gene            complement(12980..13483)
                     /locus_tag="PLG98_07980"
     CDS             complement(12980..13483)
                     /locus_tag="PLG98_07980"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOE33232.1"
                     /translation="MSWYVIHTKSRHEHKVNDGLVQKNLTTFLPEVEVWSKRKDRKKK
                     IMVPLFSSYLFVDTDLNNYETRLDILKTPGVVRILGKKENSEPVAVPDDAIRAIQKFL
                     NKKTEIFSLQYPQKGEWARITGGPFAGLEGIVVKSDLNKEMFVVSIDLLHRSVAVKVE
                     GFKITRL"
     gene            13747..14994
                     /locus_tag="PLG98_07985"
     CDS             13747..14994
                     /locus_tag="PLG98_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE33233.1"
                     /translation="MERTHNISDLEKTEYLKINLGKNILPLSAAENKYMPGAGPIEII
                     ISYKRSTAVARGIECFLKAADYYNLFSSRLIMIGENTFALQYCTDGIVINILPPVHAR
                     FDDVRIDDLREKMTHVQTLPGHPLLALTLIPVNDGHFVGFSCSHAVADGISCILFLYA
                     WNCLIEGKPFPRPSAQRLFSGNPIPSDHIEKDWMPPLSALSEEIQNRVRRGAIKTYKT
                     TEYFADEFLAEMKHQAQANNREFVISNNQIMTAWLLKKYHHHILPHTDKIVIRTPVDL
                     RTVHPGVDPMYIGYAVINSFSEFRKKEIDHMSVVETACRLKESIHHTRTKRFIQEATY
                     LSKYGIEIREEVLKQFPPYNPDTDIVSTNMTHLSDLESLFLGPDAMRIEHIGVASVKT
                     SFAMVREKNSQMSAQITSRYPLI"
     gene            15016..16335
                     /locus_tag="PLG98_07990"
     CDS             15016..16335
                     /locus_tag="PLG98_07990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE33234.1"
                     /translation="MLSSASRYNIRFARPEEIRDIIELMRPYNMHHIPSPEMPPLDDK
                     RFIVVEQNGQLLGAAGYTFVSEEIGKTTLMAVRPDCARMGLGKILQTRRMQILRGLGC
                     TKIITNTDRPETIAWYKQHFGYREIGKLPKIHSFSLEHVPEWTTLESDLTTIDVPSPV
                     QGKLLMNAALTGMKHTRSDNPYLPVTPAEIAEAALEAGKLGAAIVHLHARDVDEKPTP
                     DPSIYAEIISRIREKNADLIICVTTSGRNWSDLQKRSAVLELPDDVKPDMASLSLGSM
                     NFPSGASINPPEIIRSLALRMMERNIKPELEAFEVGMVEYAKYLIREGVLRQPVYLNL
                     ILGSLGTMSASPDNLRYLVSRLPAFSTWAASGIGRYHSKVQDMAAAMGGGIRTGLEDS
                     SMHLDADKRIPAKNKDWIKRAVEAASLNGREIATPNDVRQWLDLRKS"
BASE COUNT         4198 a         3874 c         4374 g         4064 t
ORIGIN      
        1 cttcaactgg aaacgatttc catatttgcc ggctgtggtc agaaattctt gaaaatttac
       61 gccgggcaga tttttcattt ttctgtccag ggacacaaaa aaattatcaa agtgaatcgg
      121 aataacgatt ttggcgttta gtttcagagg aacgttttcg agatatgttt gggtatgtcc
      181 cctgccgccg atacccagta aaacaatatc cgatgtgatg ccgtcataca ttccgttaat
      241 aaatccggcg ctgccgtgat gcaggattgt cccggaagca tgtgtgatca gaatggaata
      301 cgtctgtccc agtttgtaat ctcttgcgga atgccggccg gtcagcggcc ggtcgattgt
      361 cccagcgtag gggactcttc ccagaaaagc aggtccgtga gcgctttcaa taaatctgag
      421 cgtgaaagct ccgattttga tgtcccggcc gatgcccgct tccttcaaac cgctttcgtc
      481 cagggggaca ccgcgcgccg cgttcagtgc gctttttgaa ccgataaaca gggcgtttgt
      541 tttaagcgca aaaaaaggag cgtcaaggca gtgatcgaag tgggagtggc tgacaagaac
      601 agcgcgaatg ttttttgttc ccggacgggt cagccatttt tcgatagcgt cccgatcagg
      661 tttcagcgaa agtcccagaa ccacgctcag cagtcccggg cggctaatgt aggggtcgat
      721 caaaataccg tcatgatcgt cctcgatcaa tacgccggca gtgcccatcc aggtaagata
      781 caaggggtct tttttctgcg ccacgccgtg gccctccccg aaaggttcga tatttttatg
      841 gagatgcccc gattattttt ctttgcccgt tttgccgggg gatgggccgc tctgaatcag
      901 tctgctccgg gcaacctgcc gttgcgcaat cagaatgtcg cggtactgtt ccacgtccgc
      961 gagcatggtt ttatcgctgt tcataagaaa atccatcagc agcgattttt tgctgataat
     1021 gaactgttcc atattttccc gattgacggc gtcatggtaa atgcggagca tacttctgcg
     1081 caacggccgc gatgagttga aaatcagtat cagcaccata ttgttgacag ccctggcgat
     1141 catatcctgt tcgtccagcc agagttcaat gaggacgtcc cggtgatcca ggttgttcag
     1201 ttccgaatcc atcagatcgg acagcgcctt gagatcgcgc gcggaagccc tctgggcggc
     1261 cagtttcagc atttccggaa acatgatgat ccaccactcc cagacttcat cgatgacaaa
     1321 cagatcggcc gccagttcgt cttcgagggt ttctatcctg gaaaaaagcg ttcgaaagaa
     1381 atcaatcccg gcgtgtttta agaaatcttt tacataaatt ccgtctccgc ggcgaatgtc
     1441 cagcaatccc attgcctcca gttgtttcaa ggccacgcgg agtgatgttc tgtccacatt
     1501 cagggagccg gacagttcac gctccgacgg aagtttttcg ccgggctgca gtttatgaaa
     1561 aaatatgtcg cgcatcagcg cagtcgaaat cttctgatgt gcctgttgat tggtcatgtt
     1621 tcgtacgaac ctcccgaata ttttattgct ttctttcaat ttctatcaaa ctgacctgat
     1681 gcattacatg cagtcatgtc ggtaagttat cctaagatgc ccatctcaaa agtgaatcac
     1741 gtttgttgcc ggacgatgtc cgtgagattc aagaatgggt tctccgatgc gtcattgaac
     1801 taaacgtatt tattatgtat ttttttcttg acaaaatcaa gaccaaaata tatacattgg
     1861 ttaaaccaat cagaacaata gatagtaccg cactgaaagt tgaggtccat tgtaatgcga
     1921 gatgtttatg ttatcggagc tcataccatc aaattcggaa aatatattga gaagagcatt
     1981 aaggatcttg ccgcccagac ggttatttcg tgtctgaaag aagctcatct ggagaaaaaa
     2041 gacatacagg cgctgtggtt tgccaattcc ggttggggat acagcaaggg acaggactgc
     2101 atccgggcgc aagttgcgct caggccgatc gggattgacg ccattccgat gaccaatgtc
     2161 gaaaatgcct gcgccggagg atcgacggct ttccatcatg cctggttggg cattgccggc
     2221 ggtctttatg atattaccat ggccgtaggc gcggagaaac tttacgacaa caataaaatg
     2281 aaagtattcg ccggtttttt aagcggtctg gatattgaaa acctttccag cattattgcc
     2341 aacatttctt tttacggcat gtcggaacag gataaaaaag acatgcaggc ccatgtcgca
     2401 aagtatgcag cgctggccaa aaaaaacgcc gcaataaaaa ggaaaaaaaa gggcgtcggc
     2461 agtcgtttca gcgatctgct ggatatgttc aaagtctacc tgcgcctgag cgacgccgtc
     2521 ggttcggaaa ccgtcaggaa gatgagaaaa ttattttcag gcgatcattc gccctttatg
     2581 gacgtgtacg gatttgccgc tcgtcagcat atgaaaaagt acggttcgac catcgaacag
     2641 cttgcgctta ttgcatcaaa gaatcatttt cattcttcgc tcaaccccaa cgcgcaatac
     2701 accttcacga taacgcccga ggaggttctg gcggatcgca tggtcagctg gccgctgacc
     2761 cggtcgatgt gcgcgccaat cggcgacgga gcggcgtctg ccgttttatg cgaccaagca
     2821 accgtgaaaa aactggggtt gtcgtcgcag gcggtcaaaa tcagggcatc cgtgctcggt
     2881 tccggacgcg agcgctcttt tgatgatacc gatatcggtg aacgtttaag caaaatcgcc
     2941 tatgaaaaat cggggctggg acccggggat atggattttg ccgaggttca cgacgccagc
     3001 gccttcggtg agctgcatca ggcggaagcg ctgggtttct gcggtgaggg cgaaggcgga
     3061 cgtttggcgg aaagcggcgc cacccgactc ggcggcaaga ttcccgtcaa tgtcagcggc
     3121 ggtctggagt cgcggggcca tcccgtcgga gcatccggac tggcgcagat tcacgaactg
     3181 gttacccagc tttgcggcaa agccggtaaa cgacaggtcg agggcgcaaa aatcgccatc
     3241 gctgaaaacg gcggaggagc cctgggacag gaagaggccg ccatgtgcat tcacatcctg
     3301 gaggggccgt caaaataatt tgcggcgggt atgtcccggt atctcaaaat taaaaacaga
     3361 aggtgaacac gatggatctg aataaaaaat ttcctgaaaa aagagtggtg attacgggtg
     3421 cgggtagcgg tttggggcgc gctctggcat tggaatttgc caggagaaac tggcggatcg
     3481 ccatagccga aatcaatgat gagcgtgccg ccgaatccgc aaaaatggtc cgggagcagg
     3541 gcgggattcc gctgacgatt cactgcgatg tgaccctgcc ccgcgacatg gagaacgttt
     3601 taaccatcgt ccggagagaa tggaagggtg tggatattct gattaacaat gccggagtgg
     3661 cttctgccgg atggtttgaa cggattcctc tggaaaaatg ggactggatc atcggcatta
     3721 atctgatgag tatcatttac ggatgccgca cgttcatccg ttatttcaag gaatccggga
     3781 caggcggtta catcgtcaac gtggcgtcga gtgcgggaat cgcctccctg ccagagatgg
     3841 tcagttataa cacgacaaaa gctgcggtca tctcgctttc cgagaccctg cggatcgagc
     3901 tttccaaaat taacgtcggg gtttccgttg tgtgcccgac gtttttcaaa acaaatctga
     3961 tggatcagtt ttcatcgccc gatgaacgtc agcgtcagct ggccgataca tttttcggaa
     4021 acgccaaaat cacgtccgag caggttgccc gacacaccgt tgcgtctatc gagaaaaatc
     4081 gtttttatgt gattacgcag gccgacggca gaaatatgtg gcgcatgaag cgatggttnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tacgccattc ccgtacatca
     4261 aaacggattc gatgaatttt tgtaggcgtt ggcagttgaa atgtgataag agaaattaat
     4321 aaatttaaat tcataccttt tatcgggcag tagtttcgat gggaattgcc ggagacattg
     4381 tcatcatcgt catggccgcg ctgctgggcg gcctgatcgc tcagatgctg aaacagccat
     4441 tgatcctggg atatattctt gccggggtcg tcgtggggcc ttataccggc ggagtcacca
     4501 tttccgatat ccacagtatc gaaaaactgg cggaaatcgg tgtcgcgctt ctgctgttcg
     4561 ccctgggact ggaattttcc ttgagagaat taaggccggt gcgcgccatt gctcttttcg
     4621 gcacaccggt gcagattatt ctgacgacgc tttacgggtt tggcatcggt cgcatactgg
     4681 ggtggtcctg gacgtcgtcc gtgtggtttg gagcgcttat ttccctgtcc agtaccatgg
     4741 ttctgatcaa aactcttgag aaacagggga tgatggggac gctgtccagc cgcgtgatga
     4801 tcggcgttct gattgttcag gatctggcgg tcgttcccat gctgattatt ctgccgcaat
     4861 tgaacaatgt tcaggacggc atggcgattc tgcttgttgc cgcggtgaaa gcggccatat
     4921 ttctgaccgt gatgatttta gtcggaacta aaatcatccc ccggctgatg cgatacattg
     4981 ccaactggaa ttcgcgggag cttttcctta tcaccaccac caccatcggg ctgggtatcg
     5041 ggtacgcaac ctatattttc ggtctttctt ttgcgttcgg cgcttttgtg gccgggatgc
     5101 tgttgagcca gtcggattac ggttatcagg cgttgagcga catcattcct ctgcgcgacg
     5161 tattcagtct gatgtttttt acgaccatcg gcatgcttct ggatatccgg tttcttctgg
     5221 ccaactggga aataatcctg ctgctggtcg gactggtgat gctgggcaag gcggtcattc
     5281 tggccgtctt gagtcgggtc ttcggttatc ataacgttat ccctctggcg atggggctgg
     5341 gcctttcgca ggtcggcgag ttttctttta ttctcggacg cgtcggcata agtacgaaat
     5401 ccattacggg cgagttttac acgatggtgc tgaccatgac gattgtcacc atgctgctga
     5461 cgccttttat atcgggatta acaaccccgc tgtacgccat ggcccgccgc tggatgaaac
     5521 cgttgaaact cgaaccgttg aattttccca gcaaaggatt aaaagaccat attgtgattg
     5581 ccggcggcgg acgggtgggc atgtatgtcg ccaatgttct gcagcaaaaa aatattcctt
     5641 ttgtgattct ggagtataat tccaggcgtg ttcacgaagt ccggaaaagc gggttcccga
     5701 ttatcttcgg ggatgccgca aaaacgatta tcctggaagc ggctgatctg aaaagggcga
     5761 agcttctgct gatcacaacg cctgttgcgg tgatttctct gggcattgtt gcgcatgcgc
     5821 aacgcatcaa tccgggtatc cgcattatcg cgcgggcgga aggcgaggaa cagatgaagg
     5881 ccctttatac aaaaggcgtg acatatgtgg tgcagccgga attcgaagcc agtctggaaa
     5941 tcgtgaacca gacgctgctg aacctcggca ttccggcaga gcagtttaaa acgtttacgg
     6001 aggatgcccg gcaggaactg cacaggccgt tatccgtgta ggcctcctgc ggggtgtaaa
     6061 aatttattga tgaagaaagg atgattccat gaaactgaat ctgaagacga tcataagaaa
     6121 atgtcttggt ttcgtgaatt tcagaagcat gacgaaggcg accttatggt ggatgtggcc
     6181 gagacgccat cgcgtttttt ttatttttga aggccgcgaa tttacctacg gggatgttta
     6241 ccggcaggcg cagcgctatg cgcatttttt cctgtcgatc agaaaaaaac tggtcgacag
     6301 cggcaaactg gccagcgatc agagaatggc cttcggggtt tatatggaaa atacgccgga
     6361 atttcttttt gcttcttttg gagcggcctt gagcaactcg gttctttttg ccatcaatac
     6421 cggcttccgg ggagacatcc tgggcaaagt gatggaacag gcgggcatct cttttttgat
     6481 cgtcaacgac agtacgctca aagaggtgga gggtgttgtg aaaaatgtaa acatcattgg
     6541 tccggaaaat atttacgtcg tccgtgaagg agaaaaatct cttcccgacg gttttcaatg
     6601 gctggacaaa gcggtggaag aagctcaaca gatcgatgtg ccgaaccgct accgggtcag
     6661 agtggataat ttcagcccgg taattgttat ttatacatcg ggaacgtcgg gcatgccgaa
     6721 gggggtgccg tgcacgcaca tcaaaatgtt cggagccgga tttgtcgtcc agtcggcggt
     6781 tcatctcaac aggcgcgaca gagggtacat ctcgatgccg ctttttcatt ccaatgcctg
     6841 gtacatcggc atacttcccc agatgattgc gggcggcagt tttgtgctca aaagacgttt
     6901 ttccgcgagc gcttttgaag acgacatgct ggcgcacggg gtgacgttcc tcaactacgt
     6961 cggacagcct ctgcattaca tcatcgacgc gctggagaga aaatacggca gcggtgaagc
     7021 ggtggaagcc gcactggcga aacatccccg caacaggttc cgcaaggcct acggcaacgg
     7081 ggccacggtc aatgaccgca ttaaacttaa acgttatttg ggcatggacc atatttatga
     7141 aatttacggt tccaccgagg cggtgattac cacagccaac tgtcccggcg atccggtgga
     7201 ttccgtcgga cgcgtcgatg gttcggtgcg catcttggat gaagaaggaa aggaatgcga
     7261 gccgggaatg gtagatgaaa aaggcgcact gctcaattat gacaaagcgg tgggcgaaat
     7321 ctgccgcaag gtgggggaca acaatttgag gtttgatgga tattacggca acgcgggagc
     7381 caccagccag aaatttcgca atggctggta tcattccggc gacctcggtc atatcagaat
     7441 catcaagggc aagcggtata tgtttttcaa cggccgtacg gacgactgga tccgcaagga
     7501 cggagaaaat ttctcagcgg agaatgttct tgatttcgcg cagaaaattc cctgtgtgga
     7561 ttgtgccatc gcctacgggg ctccctgtga agttgccgat gaaaaggtga tgataaccgt
     7621 ccagctcaag gaaggggccg atttcaatcc cgacgaggtg catgaatggt ttatccaaca
     7681 gcagaaggag gggggaatgg atcccaagtg gatgcccgat tacatccgcg tcatcgaaaa
     7741 gttttccgta accgatacgc agaagattct tgtgcggccg ttcaagcgtg aaaacttcaa
     7801 tatagagatc aatgcctcaa tgcgcattta ttttcgccgg cgcggcgata ccgcctatcg
     7861 gctgctgacg cctgatgaat tcagaaaaat cagggaggat ttcaaaacca acggccgcga
     7921 agcaatcctg gcgtcgtgct gatggccttg gatctgttgc tttgcgggtg tcgatgacat
     7981 gaatcaggtt tttgagaaag gaggacaaca tgttgcagaa aatcattatc gcaacgatca
     8041 tttttttatt ttcattgtcg ttgtgttggg cggctgaagg gatcaaactt cccgctcccc
     8101 aaatggacgg gggcaaaaat ttgatgaatg ccttcaagga aagaaaatcg gaacggtcat
     8161 acagcgccaa aaaaattcct ctcaatgtgc tttcgaacat gctttgggcc gcctgtggaa
     8221 tcaaccgtcc cgaagcgggc aaacggactg ctccgtccgc catgaaccgt cagcagattg
     8281 acgtttatgt ggcgacggcc gatggacttt ttctttacga ggccaaggga catttgctca
     8341 agcccatcct caaccaggat atccggccgt tgacggggaa acagtcgttt gtcgcccaga
     8401 cgccggtgac cttgatttac gtggccgatt ataagaaaat gggcaacatc agcaatgaag
     8461 aaaaagcgtt ttacgcggcg acggataccg gctttatcag cgaaaacgtt tatctgtttt
     8521 gcgcctccga gggattgggg acggtggtcc gggggtccat cgataaagaa gccctggcca
     8581 aaaccatgaa actcggcgat catcaaaaaa ttgttttggc gcaatccgtc ggttatccta
     8641 aagaataata attcagaacc gcagacaccg atgcggtaag caaaggggaa accaaaacgg
     8701 aagcctgttg actgccgtgg tctgaaggat gcggcgggat cggtcatggc gggttgacgg
     8761 ccggggaggg agtatggccg cacatgacga gacgggttcc tgcggcctgt gtcgcggggt
     8821 tatagaatga tttttatcgc ccggtgagcc ttgagcaatt cgtaaagggt ctgccggtgg
     8881 gattcgcttt ccactatcac ttccacgttg agacagcgat agctgttgtt ccgactgctg
     8941 cgggaaaacg taaccagaca gtttctgtcc tgaataattt catcgatcgc cattctcatt
     9001 tcgtcctcat ccgtcccgat aattttataa atccaggcgc agggatattc gaggtgaagt
     9061 ttgttttctg tttcttccat ttctttcttc gagatttttc gaatttttta ggagttatcc
     9121 ggaaaagctg aaattagtca agcggtttat accatggccc ctctgatgaa tcatcggaat
     9181 catcaacctt tttccggcag aatccggccg gcaaccaagt tttcggcatt gctttaggat
     9241 acatcggggt tgaacggaga cgatcgggca attaacacgg caaaccaaag actcccggat
     9301 ttgatttcat ttttccttga cagaatccct gctccccttt atcgttgttt tcacaaacat
     9361 ttttgtgcaa agaaagcgga tgtagggtac gcggtgaagg aaagcaaagg cgtgaaaaga
     9421 aataatcaaa gcagcggaag ttttttcagc aaaacggcgt cagatcatga ccgcgggggg
     9481 agaaaagatc tcggaaaagg tccccgattg tatctacgaa agtggttttt gattctattg
     9541 ctgttcgttt ttctgcctgt ttcctcctca gttgctgaaa atgtcaagtg cagccgcatg
     9601 cctctcctga tgaaaagttt tctggccaac cattatgcca ttaaggatct gaacgcggag
     9661 atcaacaatc aggtcgtcga tcagatgatc aagcgcctgg acccttcaaa aacattactt
     9721 tacgattccg atgtccagaa cttaaggccg gttctgcgaa gagctttttc cagcatgcag
     9781 tcgggagact gcgccttgct caaaccggtt tacgatcttc tggtgacgcg ggcgcgcgaa
     9841 aacgaaaaaa tcataagaag gattctggga tcgaattacc ggattgacga agatgcggaa
     9901 ttgtatatcg atgtgaaaaa gcgtccgtat gtcaaaaccg cggcggaaaa atacgatctt
     9961 ctaaagaaag ttgtgcagtt ccagatcgaa aacgatttac tgtccggtgt gaccctggcg
    10021 gaagccaaaa aacagcaggt tcaccgctat gagttgcaga cggccagggt cctggagcgt
    10081 cgtcccgaaa agctcattga atatgcggcg gaggcgtttg cacaggctct ggatccgcac
    10141 accaattatc tttctcccga caatctggaa gacctcaaaa ttcatatgca gctgtcgctg
    10201 gagggaatcg gagcgctgct cagcagcgac aacggcttta ccgttatcga ggaattgatt
    10261 ccgggaggaa gcgccgagaa atcaggactt ctcaaaccca aagacaaaat cattgctgtg
    10321 gcacagaaaa gagcaaaacc ggtcaacgtg atcgacatgg acctgcgcga tgtcgtaaaa
    10381 atgattcgcg gcaaaaaagg aacgcaggtg acgctgatga tcctgaggca gtccgaacgc
    10441 acgtatcgtt tcgatatcac gctcacgcgg gacaaggttt acattaagga tcaggaagcc
    10501 aaagtcgatt accagaccag aatggtcaac gggaagaaat acagttttgc ggtcattgat
    10561 ttgccgtctt tctacggtga cgaaaaagag aacaaatcca gctatgaaga cgtccgcgcc
    10621 cttcttatgg aagcccggca gcgcggtgtt gatggggttg tcctggacct ttcgcgcaac
    10681 ggcggaggtt tgatcggcga agccgtgcag attgccgggc tttttcttgg cagggggccg
    10741 gttgtggcga ccaaggataa cagcgaaaaa ctgacggttt ttgtcaacgg cgcggcaacg
    10801 tctgaaatga aattaagaaa aaacaaaatc atcactttcc ccgcggaaga tcggccgctg
    10861 tataccgggc ctctggtggt tttgacaagc cgcctgagtg cttcagccag tgaaattgtt
    10921 gccggagcgc ttcaggacta tcgtcgtgcc atcatcgtcg gatccgatcg caccttcggc
    10981 aagggatcgg tgcagacact tgcgccgctg cccagagacc tcggggggat gaaagtgacg
    11041 acagggcttt attttctgcc gggcgggaag tctacgcaaa agacgggtgt gccggttgac
    11101 gtgcgacttc ccggtttttt tgttctggag gatgttggag aagcggaact ggaatacccg
    11161 ctgccggcgc aggccattca gccgttcatc aaggcatcgt caaattctgc tttactctgg
    11221 acgaccgtcg accatccgat ggttgcaaaa ctgtcgataa aatctcaggc ccggattgcc
    11281 aaggatccaa aatttaaaga gattattcag aaaaacaagg aggctgcgga aaagaagggc
    11341 cttatccgcc ttgttgattt gcgggaggaa cggaaaaaag aaggaggatc aaataaaaag
    11401 gaaacgcccg ccgaactgaa acaaaaagtt cgtgatcagt atgcgccgta tgtcaatgaa
    11461 agtatcaata ttctttccga tatgatgacc gcaaaggcag cgttgccgat ggcaaaggct
    11521 ctatagggta atcggttatc atcaggaagc ggacagaaaa ggagggcaaa aggcacagtt
    11581 tttggcaaac cggtttgcgg aggatcggtg gtggcgcgga aagccggtat ctgctgcgag
    11641 gttgccggaa accgcctgcc accttttaag gagtcgcccg cggcattgtc atcgataaga
    11701 aacgccaggc aaccgtaacg cgtcatgaaa ttataaggcc cccagttcga ggtagggcgg
    11761 tctttcgtcc atttcagata aaatgttgta gccgttccca gacatcatgt aaatgcaggt
    11821 ccgcgtggaa atatgcaaac ggctcttcaa cgctgatatc aggggatctt tttccatcaa
    11881 accgacaacc agaaaagctt tcccggtatc ataatggtca tggagaatgg tttgcagcag
    11941 caggttaaat atcttcggat gattattttc gatggcgatg caggcggcga caatattccg
    12001 gagaggttct ccgggttcag gcatcggcgc cgtgtgtaag atgcctgcca agggggcact
    12061 caaacgttta aaccaatgaa gatgccatgg ataatcggca atcagcatac ggcggaaagg
    12121 gagctgatcc cagcaggcca taacaccgct tattttgttc ctctccacag ccacgtaaaa
    12181 atcgtccgga tgcagccccc gcaaaatgcc gttgtctgca gtaatgtcgt cggccgtata
    12241 aacggggaaa aactgccttc ggcttccgtt acggttgaga aattcagcaa tcgcatccgc
    12301 acccaccgca gtgccgcgca caatccggag gcctttttgc ggccgcggta accggcgcct
    12361 gaataacgga atggcggcat tataaagaac acccatgtcg gtgcaatcag gaaaaccggc
    12421 ccggccgctg gtcatgatat ttctggcgcc gacattgtcg taagggatgg cggatagata
    12481 aaagcggacg ccaaatcctt cctggtgcca cttcttcagg atattgtgaa ggcggacgag
    12541 tatggtggtg tttctcagcg atgaatcgat gcgcagaccg ccgatgtatc cgaaatcttc
    12601 ggggaccccg ttgagataca gcctgcgttt tgacatcgat acgacgccga cgatccggtg
    12661 attgttttcc gcaacggctg tccggtggac gtatccttcc acgtcgatgg cggaaaagaa
    12721 actcggttga cagtccatgg tcagcgggat gcgtcccggc atgaaagtcc gcttcatcag
    12781 ggcgcatatg gcttcatcgt cctcccttcg cgcctcccga atcagggttg ggggaaaaaa
    12841 ttcaagtgca ttttttttgt ctttgtccgt cattgtcgat tatcaatatt taaggaaccg
    12901 tgaaagcgat atccccctgt tattttttct gtatgatgtg attatctcat gaaaaaaact
    12961 atgctttaaa taaaattgat taaagcctgg ttattttaaa cccttccact ttcaccgcga
    13021 ccgaacggtg taagagatca atggagacga caaacatttc tttgttcagg tcgcttttta
    13081 cgacgattcc ctcaagcccg gcaaaagggc cgccggtaat tcttgcccat tccccctttt
    13141 gcggatactg aagagagaat atttccgttt ttttgttcag aaatttttga atcgcacgaa
    13201 tggcgtcatc aggaactgca acgggttctg aattttcttt tttgcccaga atacggacga
    13261 cgccgggcgt tttcaatata tcaagtctcg tttcgtaatt gttcagatcc gtatccacaa
    13321 aaagatagct ggaaaaaaga ggaaccataa tcttcttttt tctgtctttc cttttgctcc
    13381 agacttccac ttccggcaga aaggtggtca gatttttttg gactaggccg tcgttaacct
    13441 tgtgctcgtg acgacttttt gtgtgtatga cgtaccagga catttttcaa tatacgcgat
    13501 ttttgcactg aaattataac attgccatgt gttttgcaac ggatttatcg tattttgatt
    13561 tttgcaggca cagccatcac agtacggaaa catcagggcc gatcaaatgg gataacggac
    13621 gccccaggcc ggtcggtaaa aaatccgtca cgcccgcatg gatatggccc gcttttccct
    13681 tgacatgcaa aacaatcgcc tatagaaaaa catcatatct ttgttcctgt caaaaatgaa
    13741 gttaccgtgg aaagaacgca taacataagc gatttggaaa aaacagaata tctgaaaata
    13801 aatttgggga aaaatatttt gccgctgtcc gcggcagaaa acaaatacat gcccggcgcg
    13861 ggtcccatcg aaattatcat atcgtataaa agaagcaccg ccgttgcccg ggggatcgaa
    13921 tgtttcttaa aagccgctga ctattacaat cttttttcca gccgtttgat tatgattggc
    13981 gaaaatacat ttgccctgca gtattgtacc gatggaattg tgatcaatat tctgcctccc
    14041 gtccatgcgc gttttgatga cgtaaggatt gatgatttga gagaaaagat gacgcatgtt
    14101 cagaccttgc cgggtcatcc gcttctcgcg cttacactga ttccggtgaa cgacggacat
    14161 tttgtcggat tcagttgctc tcatgcggtc gccgacggca tttcctgcat tctcttcctt
    14221 tatgcctgga actgccttat cgaagggaaa cctttcccgc gtccctctgc gcagagattg
    14281 ttcagcggaa acccaatccc ttctgatcac attgaaaaag attggatgcc gcccctgtcc
    14341 gcgttgagcg aagagattca aaaccgcgtc aggcggggcg cgatcaaaac atacaaaacg
    14401 acggaatatt ttgccgatga atttcttgct gaaatgaaac atcaggcaca ggcaaacaac
    14461 agggaattcg ttatttccaa taatcagatt atgaccgcat ggctgttaaa aaaatatcat
    14521 catcatattc tgcctcatac agataagatc gtcatcagga cgcctgtcga tctcagaacc
    14581 gtccatcctg gcgttgatcc gatgtatatc ggttatgccg tcatcaatag cttttcggaa
    14641 tttcgcaaaa aagagattga tcacatgtcg gttgtggaga cggcctgtcg cttaaaagaa
    14701 tccattcatc acacaagaac gaaacgcttt attcaggagg caacctattt gtccaagtac
    14761 ggcatagaga ttcgagaaga ggttctgaaa caatttcctc cttataatcc ggacacagat
    14821 atcgtttcaa caaatatgac gcatttgagc gatcttgaat ctctgttttt aggcccggat
    14881 gccatgcgta ttgagcatat cggcgtagca tcagtgaaaa caagttttgc catggtgaga
    14941 gaaaagaata gccaaatgtc agcgcaaatc accagccggt atccgctgat ataaatcgtc
    15001 caggaaagtt tacatatgtt gtcatccgcc tcccgttaca acatccgatt tgcccgcccc
    15061 gaagagatcc gcgatatcat tgaactgatg cgcccttata atatgcatca cattccttcg
    15121 ccggagatgc ccccgcttga tgacaagcga ttcattgtgg ttgagcaaaa cggacaattg
    15181 ctcggtgccg ccggttacac gtttgtttca gaggagatcg ggaaaacgac tctcatggcc
    15241 gtacgtcccg attgcgcccg gatggggctg gggaaaatac tgcaaaccag acggatgcag
    15301 attctgcgcg gactcggctg cacaaaaatc attacgaata ccgaccggcc ggaaaccatc
    15361 gcctggtaca aacagcattt cgggtatcgc gaaataggca aattgccgaa aattcattcc
    15421 ttcagcctgg agcatgtgcc ggaatggacc acattggaat ctgatctgac aaccattgac
    15481 gttccgtctc ctgttcaggg gaaattgctg atgaatgctg ccctgacagg gatgaagcac
    15541 acccgaagcg acaatcctta cctgcccgtt acaccggcgg aaatcgctga ggcggcattg
    15601 gaagccggga aactgggagc ggccatcgtt catcttcatg cgagggatgt cgatgaaaaa
    15661 ccgacaccgg atccgtcgat atacgctgaa attatcagcc gtatccggga aaaaaatgcc
    15721 gatctgatca tttgcgtcac cacaagcggt cggaactgga gcgatttgca gaaaaggtcc
    15781 gccgtgctgg agttgccgga tgatgtaaaa ccggatatgg cttctttgtc tctggggtcg
    15841 atgaattttc cctcgggggc cagcatcaac ccgccggaga tcatccggtc tctggccttg
    15901 cgcatgatgg aacggaacat caaaccggaa ctggaagcgt tcgaagtggg aatggtcgaa
    15961 tatgccaagt atctgatcag ggagggggtg ttgcgtcagc ctgtctattt gaatctgatt
    16021 ttaggatcgt taggcacgat gagcgcctca ccggataatc tgcgttatct tgtttcccgg
    16081 cttccggcgt tttcgacgtg ggccgcttcc ggaatcggaa gatatcacag taaagttcag
    16141 gatatggcgg ccgcaatggg cggcggtata cgaaccggac tggaagatag cagcatgcac
    16201 ctggatgcgg acaagcgcat accggcaaaa aacaaagact ggatcaagag agcagtggaa
    16261 gcggccagcc tcaacggccg tgaaattgcg acgccaaatg acgttcggca atggctggat
    16321 ttgaggaagt cgtgagccgg aaaaaagtag tttctgcctt aggaaaatgg atttcagaaa
    16381 aatggtaaaa acaacggaat tgcaacccgc tgtgtcgaaa aataaatctt ttaatcgtca
    16441 gtatccgccc gtcttttttg gaaatttcgc atcggaaaat cattcagaaa agggggaaaa
    16501 gggatgattc ctaaagaaga tcagaacgtt taaaatcata aaacgttgaa atggattggt
    16561 gccggagctc ggaatcgaac cgagatgccc ttgcgagcgg gggattttga
//
LOCUS       DAOSME010000049        16497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4186_length_16497_cov_19.944228, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000049 DAOSME010000000
VERSION     DAOSME010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4186_length_16497_cov_19.944228"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLG98_07995"
     CDS             complement(<1..1157)
                     /locus_tag="PLG98_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE33235.1"
                     /translation="MILPDYYEFCCRVKIVAGHDALEKIPALLAEMGSKKPLIITDKG
                     VAAAGLVDIVVNALKDGVTIGGIADNVPPDSDLKVVNQLAGLYRDNACDAIIAVGGGS
                     VMDTAKGVNIVVSENATDLMAFSGAGALNRRLKTLVAIPTTAGTGSEVTLVAVIKDHE
                     KHLKMAFTSYFLLPSVSILDSRMTMTMPPFITAMTGMDALSHAVEGYYCLQKNPLSDA
                     HSLAAVNMIHHYLPTVTKNPADKDGRLAMAVSACLAGISFSNSMVGMVHNLGHATGSV
                     CGVPHGICMAILLPYGLEYNMHKRGDTIGELLLPMAGEDVYLKTSKADRADKVVSLIR
                     QMNQDLNEITGGRHARFFSEIKDRDGKQMVPREILPEIAKAALLDGATIYNP"
     gene            complement(1176..2333)
                     /locus_tag="PLG98_08000"
     CDS             complement(1176..2333)
                     /locus_tag="PLG98_08000"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE33236.1"
                     /translation="MNITNGFAFICPFKTNSGNKALEHLPVELAGFNAFKPLIITSRQ
                     LAGWRAISTLKNAFGDSGMTLGLFDDVTQTADLNVVEQIRDIFVKGQYDVIIALGGGI
                     IVDVAKIVNLAVSQKVADACQLSPATAIRDPLGPLVVIPTFAVNGMETSKYAVLSGKT
                     FTSVHLMPDLVVLDPRLTRAKDGKTVAEAGIAAFGRTLEAYIAPDKNPFMDAYAFTAM
                     RFIRENLALAVKKRCNKKESLAVANAVAMSGCVISNTDQNVLHRLGQVFQNTVHVHPG
                     VIIGMCLYPLLDDTMKKGNSLVSNLLLLLGGDDKYAAMPEAGRTGAALREVYGFTYEI
                     YSMLKGKMPQTIREAGIPQYLMDDVLEVINQEPDGTYLRTVIDRVGGSTKA"
     gene            complement(2349..2870)
                     /locus_tag="PLG98_08005"
     CDS             complement(2349..2870)
                     /locus_tag="PLG98_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33237.1"
                     /translation="MREIHAGGKLLKKIYVAIFLWFTGRAIQAAAKVDKEVQKEFDAL
                     PDEFTFALGCLPNGPWMVVGKQKGKVKYFGGDLKCRPVHLRLVIKSIDAAFLILSFQE
                     STALATTRNRLMTDGEVPYALSVIRILDIVEVYLLPKIIASLAVKRYPSWWSFGRKLA
                     GRVGVYTRTLLGI"
     gene            complement(3221..3643)
                     /locus_tag="PLG98_08010"
     CDS             complement(3221..3643)
                     /locus_tag="PLG98_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33238.1"
                     /translation="MAQINFAPGVEDAGLPVMIADIIKGNLEAKPQRQGDFNALNGNI
                     YMQAHDAEVDMTLVFEKGSLTVHSGKVGDPKISISTDSDTLLALANIDVKFGLPYYFD
                     KVGFEVLKKLFLSRELKLAGLLTHPIMLTRFTKLMSCR"
     gene            complement(3687..4850)
                     /locus_tag="PLG98_08015"
     CDS             complement(3687..4850)
                     /locus_tag="PLG98_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE33239.1"
                     /translation="MAYDRDLNFIYRNPTRLIYGENSINEVGLEADALKCSRAFLVTD
                     KGIVNAGLTERVEKALGRKLAGTFDGCIQDSDLRIINDAAEIARGKGADILVSVGGGS
                     VIDTTKGIAIVLKEGGKIEDYKGQQCLTRPQTPHIVIPTTAGTGSEVTYFAVIKDQVN
                     HQKHPYCDDNIIPNVGILDPTMTAGLPPMLTATTGMDAFSHALEAIHATPCSPVADGM
                     ALHAIRLIMEYLPLCVENGKDLFARGQQLMASTIAGIAFTNAQVGLVHAIAHTLGGLF
                     QVPHGLANSLMLPHVVRYNMDTCADKYVMIAQAMNIYAKGMSDEEASEAVAEAITALT
                     TRMGVPQRLRDVGVPEDGLVQLAEGTLSDGAIVYNPKPVFEAEEVLGIIKQAW"
     gene            complement(4980..5558)
                     /locus_tag="PLG98_08020"
     CDS             complement(4980..5558)
                     /locus_tag="PLG98_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOE33240.1"
                     /translation="MPYTYGTPEWDEAYNKRVEERLANEPKPFIIFLPEWLKEWEKYM
                     QNDAKYKEVALPNWENAIVIHITPAPQFGLDHDIFVRMDLWHNGECRSIRYLPKSEVG
                     KNPKDFIITASIDRWFAVGRKQLDVVKGMMQGKLKLKGDLPTVVKAVKPAVRIVETVG
                     EVGGKYEDELTPEETEKFRATVNELLPEFGLV"
     gene            complement(5643..7520)
                     /locus_tag="PLG98_08025"
     CDS             complement(5643..7520)
                     /locus_tag="PLG98_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOE33241.1"
                     /translation="MANCESGYAKKLLEINLTEVKIQKAPLDESLLRNYLGGGGLAAH
                     LFLNRFTPDSDALSADNPLIIMTGPLVGTLLPGTSRFAVCARSPQTGFWGIGTCGGTF
                     GPELKFAGYDGIFIEGKSEKPVYLLIEDDNVEIRDAADLWGEPVYKVTDILKERHGGK
                     RKPKVLVIGPGGENLVRFAAIANDKAHYIGRTGMGAVMGSKMLKAVVVSGTGKVLPAL
                     PEEYKALRENLLEKIKENQFGQVLRSMGTAASMDPGMMMGDVPIRNWTKGEFDGSVNI
                     GGPTMTEKYLTKVHACYACPIACKRVVRVEGGPFQTEEGPGPEYETCCTFGSFMENDN
                     LEGIIKANETCNQYGVDTISCGATIAFAMQCFDSGILSTEDTDGIELNWGNIESALKM
                     VEKIAKREGIGNVLAEGSAKAAQIIGKGSEQYVVAVKGLELPAHDPRGFHGMGLEYAV
                     GYRGACHLQHMSHTIEQGMTDQADAGFKADYEGPSSDGKAEMVVLSQNIGVPPTSACL
                     CLFVYDCIAVKDFADMIHVVTGWDYSIDDLIKTGEKIWLLMRGITNLLGSRADDDTLP
                     PNVMKPLEEGMTAGSVPDIEKMLTEFYELRGLDASGLPRRDVLEKAGLNDLAQKLHGA
                     A"
     gene            7967..9307
                     /locus_tag="PLG98_08030"
     CDS             7967..9307
                     /locus_tag="PLG98_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33242.1"
                     /translation="MKKILFTLLMVFLFFTPSIFAEMVQDIPAGTAEDYYNRGNKYRE
                     QGNLDQAIYDYSKAIKANSKYSKAYYNRANSYVKQGKLAEAIEDYTKAVEINPLYTEA
                     YYNMGNTYEKQGDLAQAVASYTKALETNPRYSAAYCNRGNVYQAQGNFQQALDDYNKA
                     VELNPNFAGVYSNRGNVYQAQGNTDKAIADYNKAIELSPNFAGFYANRGNIYQSQGNL
                     QQAIADYNTAIEKNPDDTSSFYNRGLAYYGLEEYAKSLADYSTALNQNPDKEAYDDFL
                     KYFPEKRTSDNQDIRGAIVQVFEDKLNLPKKIAAPVAAAPADAIPPAAAAAVVADTKV
                     SSTPEEDIKGSVNTWLNSWQSGDMATYRSCYDERFESRKMNLDAWIDYKNNVRNRSKN
                     ISIQIDDLKISVDGDRAKAVFTQSYSSSLLKDKGTKTLELKKVGDNWKIYREIM"
     gene            9423..10043
                     /locus_tag="PLG98_08035"
     CDS             9423..10043
                     /locus_tag="PLG98_08035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33243.1"
                     /translation="MNISKQKIDQVKGFLDEREAACLYRLALEAGKKGPCLEIGSYCG
                     KSSVYLGTACKENGTVLFSLDHHTGSEEQQPGEEYFDPDLLDRESGKIDTFKFFRKTI
                     FDFDLDDVVIPVVGRSATIGRVWKTPLSLIFIDGSHAYDSVLSDYEIWGKNLMPGGYL
                     LFHDIFSDPAKGGQAPYRVYQQAVDSGRYTEMPMCESLGILIRKQE"
     gene            complement(10046..11620)
                     /locus_tag="PLG98_08040"
     CDS             complement(10046..11620)
                     /locus_tag="PLG98_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33244.1"
                     /translation="MRIAVIAPPYPLEEMPSPPLGVTYVAAAFAGAGADVRIFDYIVS
                     GYSREKIENQLASFSPDAVGATSVTLNFYDAQKILRDVKQYNHEIITIMGGPHVSFTV
                     EETLKHYPEIDLIVIGEGEATIAELTPVLRQKEKWREVRGIAYREGDEIVRTPKRDFV
                     TDIDNIPLPARHLLPISRYRALGFPVSLITGRGCPYSCIFCLGRKMVGSKVRKRNSRL
                     VLDEIEQIIGYGFDRINIADDLFTTDKERVREICAGIKERSLKFPWSAFARVDTVSQE
                     VFDLMAEAGCDSVSFGVETGNPDMLKRIKKGIRLEQVYSAVKMCQQAGMIAHASFIIG
                     LPGETKETLHQTDQFAKSTKAIYGYHFLAPFPGTTVREKIGDYDLQILTDDWSRYDAN
                     DAIVRTSFLNPEEMKAFGDGYEKEMTDDWNKIVRRYHEGRGSVEDKMQVEGHWRMKLT
                     YRLLKEDIIEQCGLIEPECCRGSKENALRELCNRIGKITQADPGVIDNTLYDFAGRGY
                     LKAEMSDQGCRWSWSK"
     gene            complement(11624..12346)
                     /locus_tag="PLG98_08045"
     CDS             complement(11624..12346)
                     /locus_tag="PLG98_08045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE33245.1"
                     /translation="MRKDHRPYYIKRFYNRFQKWYARYYIAPQCEYFGRGLVFMKPWH
                     VEIFGGPVSLGDYTTVIATSDRKVRFTVWPVWMGQGKIEIGQCCLICPGTRIMSATSV
                     TMGDGCMTAQSVSIGDADWHDIYDRSMPVGNTQAVTIGNNVWIGDSAMVSKGVTIGDN
                     AVIGAGSVVVKDIPANAIAAGNPARVIKYLDPDRPIRTRKDWLSDPAGLARDFEVIDR
                     AIMKGNTMAGWFRSVLFPRQGD"
     gene            complement(12441..13475)
                     /locus_tag="PLG98_08050"
     CDS             complement(12441..13475)
                     /locus_tag="PLG98_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33246.1"
                     /translation="MELELSVSEASAPVSVEKIGSFIISLQKENGEIPWSAGGKTDPW
                     DHVESAMGLSAAGFYTEAEKAYEWLASTQLEDGSWWSEIQDGKIINSCKESNFAAYVA
                     VGVFHHYLVTRDEKFLKKMWHTIAQGIQFAINLQAPDGEIYWARNKHGAVDKMALLTG
                     SSSIYMSIKCALAVADRLGKKRAGWRKAMDALGAAIKNRPDLFNMIKSRYSMDWYYPI
                     LCGAVTGAEAQKRIAHLWEKFVVPDWGVRCVSDRPWVTMAETAEFILTLASIGDLARA
                     KTIFSWLHDKRFSDGSFWMGVTFPDGVIWPEEKTGWTAAAVILASDALNEMTPAGKLF
                     NHQFWASISK"
     gene            complement(13481..14182)
                     /locus_tag="PLG98_08055"
     CDS             complement(13481..14182)
                     /locus_tag="PLG98_08055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33247.1"
                     /translation="MITVEIDKLDLKPDSVVLDAGCGLGRHLRHLARIPDLKIFGIDK
                     NTWALQETAKSMDTQPDARSKDYLVSIADINRLPFADASFDCIICSEVLEHIPDHQKA
                     IQELDRILKPNGTLVVSVPRYFAERICWFISRDYHNEEGGHIRIYRKKQLHRMLTGQG
                     FECWKINYKHALHSPYWWLKCLVGLKNEDHPLVRSYRKFLEWDIMKKPRPVRMLEELL
                     NPLMGKSIVYYLRKG"
     gene            complement(14163..15422)
                     /locus_tag="PLG98_08060"
     CDS             complement(14163..15422)
                     /locus_tag="PLG98_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE33248.1"
                     /translation="MKGKSKSQPLKICLLTYRGNPNCGGQGVYIKYLSKALSDLGHEV
                     DVLSGPPYPHLDPPVRLHRLPSLDLYNVEHPFRVRNARALMNPLNMYEFLTMCTGRFP
                     EPYTFGVRAYNYLQRHRKKYDIVHDNQCLAYGIGKIAQNVVPTIATIHHPITVDRQEE
                     FKSATSKRKINKIKKWYSFIRMQKEVARQLSHIVTVSECSKIDIAEEFSIPSSKFRVV
                     PNGINKEFFYPVQNGSRPQNSIIVTNSADTPLKGLRYLLEAVYRIRKKQPVKLTVIGE
                     PKKNGVVKKLVAELGIEDIVHFTGRIANEEFADYYSKATIAVVPSLYEGFGLPAAEAM
                     ACGVPLVSTSGGALPEVVGDAGIIVPPADADALAREILFLFNHPDQRKKMAQAGIARV
                     DAIFNWSKAAGDMVEVYREAIHDYRGN"
     gene            complement(15412..16068)
                     /locus_tag="PLG98_08065"
     CDS             complement(15412..16068)
                     /locus_tag="PLG98_08065"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOE33249.1"
                     /translation="MIDLHTHSIFSDGELIPAELAQRAWAAGYKFLAITDHADHSNID
                     FIIPRIIRACFKINQAGKINVFPGVEITHVAPAQIAGLANEARKLGAKVVVVHGETIV
                     EPVPAGTNLAALKADIDILAHPGLLTEKEARLAAERGIYLEITTRRGHSFTNGHVAQT
                     ARKCKAKLMLNNDAHAPADFAGRERAQRIASGAGLNAREVSAMFENSRRMVKKLQYER
                     "
     gene            complement(16181..>16497)
                     /locus_tag="PLG98_08070"
     CDS             complement(16181..>16497)
                     /locus_tag="PLG98_08070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOE33250.1"
                     /translation="FKMPVRPEYLQGAKAMQGGLMVALADETIAHAMMTQLTPEEGLT
                     TIELKSNFLAGVSGGELTATATVFKKGKSLMIGDCLVTDNKDKNVCRVSATFLLLKEK
                     SK"
BASE COUNT         4044 a         4458 c         3851 g         4144 t
ORIGIN      
        1 ggattataaa tggttgctcc gtcaagcagg gctgctttgg caatttcagg aagaatctcc
       61 cgtggaacca tctgcttccc atcgcggtct ttgatctccg agaagaaccg cgcatgtcgc
      121 ccgccggtga tctcgttgag atcctgattc atctgccgga tcagggaaac aaccttatcc
      181 gcccgatccg ccttggaggt cttgagatag acgtcctcgc ccgccatggg cagcagcagt
      241 tccccgatgg tatccccgcg cttatgcata ttgtattcga gaccataggg caataaaatg
      301 gccatgcaga ttccatgagg aacgccacag acagaaccgg tggcatgacc gagattatgg
      361 accattccga ccatggaatt ggagaaagag atgccggcaa gacacgcgct taccgccatg
      421 gccaggcgac cgtctttatc cgcaggattt ttggtcaccg tgggcagata gtggtgaatc
      481 atattgacgg cagccagaga gtgggcgtca ctcaacggat ttttctgaag gcagtaatat
      541 ccttcaacgg catggctcaa ggcatccata cccgtcatgg ctgtaatgaa aggcggcatg
      601 gtcatggtca tccgggaatc gagaatggat acactgggca acaaaaaata actggtaaag
      661 gccattttta aatgcttttc atgatctttg atcacggcaa ccagtgtgac ttccgatccc
      721 gttcccgccg tggtcggtat ggcaacgaga gtcttcaggc gccgattcag ggccccggcg
      781 ccgctgaagg ccatcagatc ggtggcattc tcggaaacga cgatgttgac ccctttggcc
      841 gtatccatca cggaaccgcc gccgacggcg ataatggcgt cacaggcgtt atcccggtaa
      901 agaccggcga gctggttgac caccttcaaa tcggagtccg gaggaacatt gtcggcaata
      961 cctccgatcg taacaccatc cttgagagca ttgacaacga tatccaccag accggcggcg
     1021 gcaacgcctt tatcggtgat gatcagcggt ttttttgacc ccatttcggc aagcagcgcc
     1081 ggtatctttt ccagcgcgtc gtgtcccgca acaattttta cacggcagca aaattcataa
     1141 taatccggta gtatcatggg ttatatcctt tccgttcagg ccttggttga gccgccgaca
     1201 cgatcgatga ccgtgcgcag gtaagtcccg tccggctctt gattgatgac ttccaataca
     1261 tcgtccatca ggtactgcgg gatacccgct tccctgatgg tctgcggcat tttgcccttc
     1321 agcatactat aaatctcata cgtaaatcca tatacctccc tcagagccgc tcctgtcctc
     1381 cctgcctccg gcatggcggc gtatttatcg tctccgccca gcagaagcag taggttggat
     1441 acaagagagt tgcccttttt catggtatcg tcaagaagag gataaaggca cattccgatg
     1501 atcacgcccg gatgaacatg aaccgtattc tgaaatacct gccccagtct gtgaagcacg
     1561 ttttgatcgg tattggaaat aacacatccc gacatggcca cggcattggc caccgcaagg
     1621 gactcttttt tattacagcg tttcttgacg gccaaggcga ggttctcccg gataaagcgc
     1681 attgcggtaa aagcataggc gtccataaaa ggatttttat ccggagcaat gtacgcctcc
     1741 agggtgcggc cgaatgcggc aatacccgct tcggcaacgg ttttcccgtc ttttgcccgg
     1801 gtcagtcgcg gatcaagcac cacgagatcg ggcattaaat gaaccgaggt aaatgtcttt
     1861 ccgcttaaaa cggcatattt cgacgtctcc attccgttga cagcaaacgt cggaatgacg
     1921 accaggggtc ccaggggatc gcgaatggcc gttgccggcg aaagctgaca cgcatcggca
     1981 actttttgtg aaaccgccag gttcactatt ttcgccacat caacaatgat cccgccgccc
     2041 agggcgatga tgacatcgta ttgccccttg acaaatatgt ctctgatttg ttcgacaacg
     2101 ttcaaatcgg ccgtctgtgt tacatcatca aaaagaccaa gcgtcattcc cgaatcaccg
     2161 aacgcgtttt tcaacgttga aatggccctc cagcccgcca actgacggct ggtgatgatc
     2221 aggggcttga aagcattgaa gccggccaac tctacgggaa gatgctccag agctttgttt
     2281 cctgaattcg tcttgaacgg acagataaat gcaaaaccgt ttgtaatatt cattttatat
     2341 tcctttcatt aaatccccaa caaagtgcgc gtgtagacgc cgacccgtcc tgcgagtttg
     2401 cggccgaacg accaccatga gggataacgt ttcacagcca gagaagcgat gatcttgggc
     2461 agaagataaa cttccacaat atcaagaatc cgtatgaccg acagggcata aggaacttcg
     2521 ccgtccgtca tcagacgatt acgggttgtc gccagagccg tgctttcctg aaaagacaat
     2581 attaaaaatg cggcatcaat acttttgata acgagacgaa gatgaacagg ccggcatttg
     2641 agatcgccgc cgaaatattt taccttgccc ttttgcttgc cgaccaccat ccacggaccg
     2701 ttgggcagac aacccagcgc aaaggtgaat tcgtcaggaa gagcatcaaa ttctttttga
     2761 acttccttgt cgacttttgc cgccgcctga atggctctgc cggtaaacca caggaatata
     2821 gccacgtaaa tttttttcag aagtttccct cccgcatgaa tttcccgcat aatcaaatcc
     2881 tcccccttat cttctcttgg aaaacagcat gatgctgcat atgctttaac atatctagag
     2941 attattgaat ctaacattca ttacaacggg catgacactc attgatgaga aaccaaagaa
     3001 tctaatgcga attataacgg acatgagtct tcttttttgc tgtgatgcga tttagattag
     3061 aaccattttt tttacgatat aaacaatttt tgatttgaag tcaaagtaat ttttttgggg
     3121 tcgtcagacg caataagacg gtttttaaca acaacgcgcc tgaatgcgcg cgctgttgtt
     3181 aaaattttta aaatgatcta tccatggcgt ttggcaggct tcaacgacag gacatgagct
     3241 ttgtaaatct ggtcagcatg atcgggtgag ttaagagtcc tgcgagttta agctcccggc
     3301 ttaaaaacag cttcttcaac acttcgaaac cgactttatc gaaataatag ggcaggccga
     3361 atttgacatc aatattggcc agtgccagca gtgtatccga atcggtgctg atggatatct
     3421 tgggatctcc caccttgccg ctgtgcacgg tcagactgcc tttttcaaag accaaggtca
     3481 tgtcgacttc cgcgtcatgc gcctgcatgt agatattgcc gttgagggca ttaaagtcgc
     3541 cctgccgctg cggtttggcc tccaaattgc ccttgatgat atcagcaatc atgacgggca
     3601 gaccggcatc ctccacaccg ggggcaaaat tgatctgagc catatctcct ccttcatatc
     3661 tttgaatgaa tattaatgaa tccaaactac catgcctgtt tgatgatgcc gagaacctct
     3721 tctgcttcaa aaacaggttt gggattataa acaatggcgc catcggataa cgtcccttca
     3781 gccaactgaa ccagaccgtc ttcaggaaca ccgacatccc tcaaccgttg aggcactccc
     3841 attctcgtgg ttaatgcggt tatggcttcg gccaccgctt cgctcgcctc ttcatcactc
     3901 atccccttag cgtaaatatt catggcctgg gcgatcatga catatttatc cgcgcaggtg
     3961 tccatgttgt aacggacgac atgcggaagc atcaggctgt tggctaatcc gtgaggcacc
     4021 tggaaaagcc cacccagcgt atgggcgatg gcgtggacaa gaccgacctg ggcattggta
     4081 aaggcgattc cggcgatggt ggacgccatt aactgctgac cccgcgcgaa aagatctttg
     4141 ccgttctcta cgcacagggg cagatattcc ataattaagc ggatggcgtg caatgccatg
     4201 ccgtccgcaa ccggcgagca gggtgtcgca tgaatggcct ccagtgcgtg actaaaagcg
     4261 tccatgcctg tggtggccgt cagcatcggc ggcaatcccg cggtcatggt cggatcgaga
     4321 atcccgacat tgggaatgat attatcatcg caataggggt gtttctggtg gttgacctga
     4381 tccttgatca ccgcaaagta cgtcacctca ctgccggtgc ctgccgttgt gggaatgaca
     4441 atatggggcg tttgcggacg cgtaagacac tgctgcccct tataatcctc aattttaccg
     4501 ccttccttta aaacaatagc aatccctttg gttgtatcaa tcacgctgcc gccgccgaca
     4561 ctgaccagaa tatcggctcc cttgccccgg gcaatttcgg ctgcatcatt aataatgcgc
     4621 aaatcggaat cctgaatgca cccgtcaaaa gttcccgcca gttttctgcc cagcgctttt
     4681 tccacccgtt cggtcagtcc ggcattcacg atgcccttat ccgtcaccaa aaaggccctg
     4741 ctgcatttca gcgcatctgc ttccagtccc acttcgttga tcgaattctc cccgtaaatt
     4801 aatcttgtcg gattgcgata gatgaagttg agatccctgt cgtacgccat agttcacctc
     4861 tccttgaatg attgagtcat taatttatga gcatattgaa acgaaattat aaaaatcaga
     4921 caaacaccgg atgtcggcga aataactcct tcctccatcc ggtgccacga agaccgtttc
     4981 taaacaagac caaattcggg cagcaattca ttaacggtgg cccggaattt ttccgtttct
     5041 tcaggcgtca gttcgtcctc atacttccct cccacttcac ccactgtttc cacaatgcga
     5101 acggcgggct tgaccgcctt gacgaccgta ggaagatctc ccttgagttt gagtttcccc
     5161 tgcatcattc ccttgaccac atccagttgt tttctcccga cggcaaacca gcggtcaatg
     5221 gaggcagtga taataaaatc tttgggattt ttcccgactt cagatttggg gagataacgg
     5281 atggagcggc attcgccgtt gtgccaaaga tccattctca caaaaatatc atgatccaga
     5341 ccgaattgag gcgccggcgt aatatgaatg acgattgcat tttcccagtt gggcagggcc
     5401 acttctttgt acttggcatc attttgcata tacttttccc actcttttaa ccattcaggc
     5461 aaaaaaatga taaaaggttt tggctcatta gccagacgct cttccactct cttgttgtaa
     5521 gcttcgtccc attccggtgt tccatacgtg tacggcatat cctttctcct tttccaaatt
     5581 ttattttatt gaaaaccttc cattccgata cgtttgtgga atggatcagt tgaacaatcc
     5641 cgctatgcag caccatgaag tttttgagcc aggtcattga ggcctgcttt ctccagaaca
     5701 tctctgcggg gaaggccgct ggcatcaagc cctcggagct cataaaattc tgtgagcatt
     5761 ttctcgatgt cgggaaccga cccggcggtc atgccttctt ccagcggttt catgacattc
     5821 ggcggcagcg tgtcgtcatc cgcccgtgag ccgagcagat tcgttatccc gcgcatcaaa
     5881 agccagatct tttctccggt tttgatcaaa tcgtctatgg aataatccca gcctgtcacg
     5941 acatgaatca tgtccgcaaa atctttaacg gcaatacagt catacacaaa aagacataaa
     6001 caggccgacg tgggcggcac gccgatattc tgagaaagga caaccatctc cgcctttcca
     6061 tcgctggagg gaccttcata atcggctttg aacccggcat ccgcctgatc cgtcattccc
     6121 tgttcaatgg tatgactcat gtgttgaagg tggcaggcgc cgcgatatcc cacagcgtat
     6181 tccagtccca taccgtgaaa cccgcgggga tcatgcgccg gcagttccag tcccttgacc
     6241 gccaccacat actgttcgga acccttgccg ataatttgcg ccgccttagc ggaaccttcc
     6301 gccaggacat ttccgatgcc ctcgcgcttg gcaatctttt cgaccatttt cagagcggat
     6361 tcgatatttc cccagttcag ttcgattcca tcggtatcct ccgtggaaag aataccgcta
     6421 tcaaagcact gcatcgcaaa ggcaatcgtg gccccgcagg aaatggtatc tacaccgtac
     6481 tggttgcagg tctcattggc cttaataatc ccctccagat tatcattttc cataaaggag
     6541 ccaaatgtgc agcaggtctc gtactccgga ccgggcccct cttcggtttg aaacggtccg
     6601 ccttccactc tgacgacgcg cttgcaggca atcggacagg catagcaggc atgcaccttg
     6661 gtcaggtatt tctcggtcat cgtcggtccg ccgatgttca cggagccatc aaactcaccc
     6721 ttggtccagt tacggatcgg cacgtctccc atcatcattc ccgggtccat cgaagccgcc
     6781 gttcccatgc tgcgcaaaac ctggccgaac tggttttctt tgattttctc aagaaggttt
     6841 tcccgaaggg ctttgtactc ttccggtaaa gccggcaaaa ccttgcctgt accgctgacc
     6901 acaacggctt taagcatttt agagcccatg acggctccca taccggttct gccgatgtaa
     6961 tgcgctttgt cattggcaat ggccgcaaag cgcaccagat tttctccacc gggtccgatc
     7021 accagcactt tcggtttgcg cttgccgcca tgccgttcct ttaatatgtc ggtcacctta
     7081 taaaccggtt ctccccacag atccgcagcg tcgcggattt cgacgttgtc atcttcgatc
     7141 aaaagataaa ccggtttttc tgatttccct tcgataaaaa ttccatcgta tccggcaaac
     7201 ttgagttccg gtccgaacgt accgccgcat gtgccgattc cccagaaacc ggtttgcggc
     7261 gatcgggcac acacggcaaa acgggatgtg cccggaagca atgttccgac aagcggtccg
     7321 gtcatgatga ttaacggatt gtcagcggag agggcatcgg agtcaggggt aaagcggttg
     7381 agaaagaggt gcgctgccag accgcctcct cccaaataat ttcttaaaag agattcatcc
     7441 agaggcgcct tttgaatttt tacctcggtg agattgattt ccagcagttt ttttgcatat
     7501 cctgattcac aatttgccat accgtctcct gaaaacaata attcagtttc ctgcttgtaa
     7561 ttatgggaac ttttcctgaa taataagaac gtgtattgaa gaagacattc aacagccttg
     7621 cagatgcttc aacatcttca ataatacagc ggtgttttca taaaatcccc ccagatttat
     7681 gaactacact gaatgacaat tttttacaga atgtgccgct tctaacacaa ttcgatttgt
     7741 tacacaagat gaaattcaaa cagattaatt tttttcaacg gtcctttatg ctttttctaa
     7801 catttgatgt atcagccgtc ttttgctgtt tcaaggctcg caacaaacct gatgaatcac
     7861 gttcccggca agaattagct tgacaacacc aacatttttt gtttaatttt ctgcactttt
     7921 caaggatatc aaccaaaatt aatttaaaaa aaggaaggca ataattatga aaaagatatt
     7981 atttactttg ttaatggtat ttttattttt cacgccatca atttttgccg aaatggtcca
     8041 ggatatcccc gcgggaaccg cggaagatta ctacaatcgg ggaaataaat acagagaaca
     8101 gggtaatctc gaccaggcta tctacgatta ttccaaagcg atcaaggcga actccaaata
     8161 ctccaaggct tattacaacc gcgccaattc ctatgtcaaa cagggcaaac tcgctgaagc
     8221 cattgaagac tacacaaagg ctgtggaaat caatcccctg tacacggaag cctactacaa
     8281 catgggcaat acttacgaaa agcagggcga cctggctcag gcagtcgcca gttatacaaa
     8341 ggctctggaa accaatccca gatactcagc cgcatactgc aacagaggca atgtgtatca
     8401 ggcacagggt aatttccaac aggcgcttga cgattacaac aaagccgtcg agcttaaccc
     8461 gaattttgcc ggcgtgtatt ccaacagagg caatgtgtat caggcacagg gcaacaccga
     8521 caaggccatt gccgactaca acaaagccat cgaactcagc ccgaattttg ccggatttta
     8581 cgccaaccgg ggcaatattt atcagagtca gggaaatctt cagcaggcca tcgccgacta
     8641 taataccgcc atcgaaaaaa atcccgatga tacgtcctcc ttctacaaca gagggctggc
     8701 ttattacggc ctggaagaat atgcaaaatc gctagccgat tactcaacgg cgcttaatca
     8761 gaatccggac aaggaagcct atgatgactt tcttaaatat ttcccggaaa aaagaacatc
     8821 cgacaaccag gatatccggg gggccattgt tcaggtcttc gaagataaat taaatttgcc
     8881 gaagaaaata gccgctcctg tcgccgccgc tcccgccgat gcgataccgc cggcagccgc
     8941 ggcagcagtc gttgccgaca caaaggtgtc ttcaacgcct gaagaggata tcaagggttc
     9001 cgtcaacacg tggcttaaca gctggcaatc gggagacatg gcgacttacc gcagttgtta
     9061 cgatgaacgt tttgaatcca ggaaaatgaa tctggatgcc tggatcgatt ataaaaataa
     9121 tgtacgcaac agaagtaaga atataagcat tcaaattgat gatttgaaaa tatccgtgga
     9181 cggagatcgc gcaaaggccg tctttacgca atcctacagt tcctcgctcc tcaaagacaa
     9241 agggaccaaa acccttgagc tcaaaaaagt gggcgacaac tggaagatct atcgggaaat
     9301 catgtaaatc ggacggtggg atgaagcgcg ttttgacata aaagtccatt gtaacataaa
     9361 accaaatggc aaatgctttt taaaaagcat ttgccatttt cattgatccg gcaacgtcag
     9421 caatgaacat aagcaaacag aagatcgatc aggtcaaagg atttctggac gaacgcgaag
     9481 cggcctgtct gtaccgtctg gccttagaag ccggcaagaa gggaccttgt ctcgaaatcg
     9541 gaagctactg cggcaagtct tccgtctatc tgggcacggc ctgtaaagaa aacggaaccg
     9601 ttttgttctc ccttgatcac cacaccggtt cagaagaaca gcagccgggc gaagaatatt
     9661 tcgatcccga tttgctggac agggaaagcg gaaagatcga tacgtttaaa ttcttccgga
     9721 aaaccatttt cgattttgac ctggacgatg ttgtcattcc cgtagtcggc cgttcggcaa
     9781 ccatcggccg cgtgtggaaa acgcccctga gcctgatatt cattgacggc agccacgcct
     9841 atgactccgt gctcagcgat tacgaaatct ggggaaaaaa tctgatgccc ggcggatatc
     9901 ttttatttca tgatatcttt tccgatccgg cgaaaggcgg acaggcgcct taccgggttt
     9961 atcagcaagc ggtagattcc ggacgttaca cggaaatgcc catgtgcgaa tcactgggaa
    10021 tcctgatccg caagcaggaa taatttcatt tggaccagga ccagcggcat ccctgatcgg
    10081 acatttcagc tttcaaatat ccgcgtccgg caaaatcgta aagggtattg tcaatcacgc
    10141 cggggtccgc ctgggtgatt tttccgattc tgttacaaag ttcccgcaac gcgttttctt
    10201 tcgagccccg acagcattcc ggctcgatca agccgcattg ctcgatgatg tcctctttca
    10261 gaagccgata ggtcagtttc atccgccagt gtccttctac ctgcatttta tcttcaacgg
    10321 agccccggcc ttcgtgatat ctccggacga ttttgttcca gtcgtctgtc atttcttttt
    10381 catagccgtc tccgaacgcc ttcatctctt cgggattcag gaaggatgtt ctgacaatcg
    10441 cgtcgtttgc gtcgtagcgc gaccagtcgt ccgtgaggat ctgcagatca tagtcgccaa
    10501 tcttctcccg caccgtcgtt cccggaaaag gcgccaggaa atggtaaccg taaatcgctt
    10561 tggtactctt ggcaaactga tcggtttgat gaagggtttc cttcgtctct cccggcagtc
    10621 cgataataaa ggaagcatgg gcaatcatgc ccgcctgctg acacattttc accgccgaat
    10681 agacctgctc cagccggatc cctttcttga tccgtttgag catgtccgga tttcccgttt
    10741 ccacgccgaa actgacgctg tcgcatccgg cttcggccat caggtcaaag acctcctggc
    10801 tgaccgtatc cacgcgggca aaagcgctcc agggaaattt caacgatcgt tccttgattc
    10861 ccgcacagat ttcccggacc ctttccttgt cggtggtaaa gagatcatcg gcaatattaa
    10921 tgcggtcgaa accgtaaccg atgatctgtt cgatctcatc cagaaccagg cgggagtttc
    10981 gtttgcgaac tttggagccg accattttgc gtcccagaca aaaaatgcac gaataaggac
    11041 atccgcgacc ggtgatcaga ctgacgggaa atcccaatgc gcgataccgt gaaatgggca
    11101 ataaatgcct tgccggcaga ggaatattat cgatgtccgt tacgaaatct cttttaggcg
    11161 tgcggacgat ctcatcgcct tcccgatagg cgattcccct cacctcgcgc catttttctt
    11221 tctgcctgag cacgggcgtg agttcggcaa tggttgcctc gccttcgccg atgacgatca
    11281 gatcgatttc cggataatgc ttcagcgttt cctcgaccgt gaacgatacg tgcggcccgc
    11341 ccatgatggt gatgatctca tgattatact gtttaacatc acgcagaatc ttctgcgcat
    11401 cgtaaaaatt caaggttacc gatgtcgccc cgacggcatc gggtgaaaac gacgccagtt
    11461 ggttttcaat tttttcacgg gaatatccgg acacaatata atcaaagatc cggacatccg
    11521 caccggcacc ggcgaaggcc gcggccacat acgtcacgcc cagcggcggc gacggcattt
    11581 cttcaagcgg atatggcgga gcaataacag ctatacgcat ttttcaatct ccctgacgag
    11641 gaaacagcac ggaccggaac cagccggcca tggtatttcc cttcataatt gcacgatcaa
    11701 tcacttcaaa atcccgggcc agcccggcgg gatcggacag ccagtctttt ctcgttctga
    11761 tcggcctgtc cggatcaaga tatttgatga ccctggccgg attgcctgcc gcgatggcgt
    11821 tggcgggaat atccttcacc accaccgatc cggcgccgat gacggcgtta tcgccgatcg
    11881 tcaccccttt ggaaaccatg gcactgtcgc cgatccagac attgttgccg atcgtcaccg
    11941 cctgcgtgtt gccgaccggc atgctgcgat cgtaaatatc atgccaatcc gcatcgccga
    12001 tactgacact ttgggccgtc atgcagccgt cacccatggt aaccgatgtc gcggacatga
    12061 tgcgtgtccc gggacagatc aggcagcatt ggccgatttc aatcttgccc tgccccatcc
    12121 agaccggcca gaccgtaaac cgcaccttcc ggtcggaggt ggcaatcacc gtcgtgtaat
    12181 cccccaggga aaccggcccg ccgaatattt ccacatgcca cggcttcata aataccagcc
    12241 cgcgtccgaa atattcgcat tgcggagcga tataataacg cgcgtaccac ttctgaaacc
    12301 tgttatagaa gcgtttaatg taatatggac ggtgatcttt tctcactgct gaaccttttg
    12361 atattggatt attgtagcag atatttttta aaaaccgggc ggggacatca tcggaaacag
    12421 ccgctttgca aaaagcattt tcacttgctt atcgacgccc agaattgatg attgaaaagc
    12481 tttcctgccg gcgtcatttc attcagagcg tccgaagcca gaatgacggc tgccgccgtc
    12541 cagcccgttt tttcctccgg ccagataacg ccatccggaa acgtcacccc catccagaac
    12601 gagccgtcgg aaaatcgttt atcatgaagc cagctgaata tggttttcgc ccgggcaaga
    12661 tcgccgatcg acgccagcgt cagaataaat tccgccgtct cggccatggt cacccacgga
    12721 cggtcgctga cacagcgcac gccccagtcg ggaacaacaa atttttccca taaatgggcg
    12781 attcgtttct gcgcctccgc accggtgacg gctccgcata aaatcggata ataccagtcc
    12841 atcgaatagc gcgacttgat catgttgaag agatccggcc tgtttttaat tgccgcgccc
    12901 agcgcgtcca tagccttgcg ccagcccgcg cgttttttcc ccagccggtc ggctaccgcc
    12961 agcgcgcatt taatgctcat gtaaatggaa ctggagccgg tcagcaacgc cattttatcc
    13021 accgcgccgt gtttgtttct cgcccagtag atttcgccgt cgggcgcctg cagattgatc
    13081 gcaaactgaa tcccctgcgc aatggtatgc cacatctttt tcaggaactt ctcatcgcgc
    13141 gtcaccagat agtgatggaa gacgccaacc gcaacatagg cggcaaaatt cgattctttg
    13201 caggaattga taatttttcc gtcctgaatt tccgaccacc aactgccgtc ttcaagctga
    13261 gtcgacgcca gccattcata ggccttttcc gcctccgtgt agaaaccggc cgcggaaagc
    13321 cccatggcgc tttctacatg atcccacgga tcggtctttc cgccggctga ccaggggatc
    13381 tcgccattct ctttttgaag cgaaataata aaagaaccga ttttttccac agagaccggg
    13441 gcgctcgctt cactgacgga gagttcaagt tccattccaa tcaacctttt ctaagataat
    13501 agacaatgct cttgcccatc aacggattca gcaattcttc gagcatccgc accggccgcg
    13561 gttttttcat aatgtcccac tccagaaatt tccgataact tctgaccagc gggtgatctt
    13621 catttttgag gcccaccagg cacttgagcc accagtacgg tgaatgcaac gcatgcttgt
    13681 aattgatttt ccagcactcg aatccctggc ccgtcagcat tctgtgcagc tgtttttttc
    13741 tgtaaatccg gatatggccg ccttcttcat tatgataatc gcgggaaata aaccagcaga
    13801 ttctttcggc gaaataccgc ggcacactga ccacgagcgt cccgttcggt ttcaatattc
    13861 ggtccagctc ctggatggct ttttgatgat cgggaatatg ctccagaact tccgaacaga
    13921 tgatgcagtc gaaagacgcg tcggcaaaag gcagccggtt gatatcggca atggacacca
    13981 gataatcctt cgaccgggca tcgggctgcg tatccatgga tttcgcggtt tcctgaagcg
    14041 cccaggtgtt tttatcgatg ccgaatattt tcagatccgg aattctcgcc agatgacgca
    14101 gatgtctgcc cagtccgcag ccggcatcga gaacgacgct gtcgggcttt aaatcaagtt
    14161 tatcaatttc cacggtaatc atgtattgcc tctcggtaca cttccaccat gtcgccggca
    14221 gcttttgacc agttgaaaat ggcatcgacc cttgcgatac cggcctgggc catttttttt
    14281 ctttgatcgg gatgattgaa caagaacagg atttctctgg ccagagcatc tgcatccgcg
    14341 ggaggtacaa taattccggc atctcccaca acttccggca gggcgccgcc gctggtgcta
    14401 acgagaggca cgccgcacgc catcgcctcg gccgcgggca ggccgaatcc ttcatataaa
    14461 gacggaacaa cggcaatcgt ggctttggaa taataatcgg caaattcttc attggcgatg
    14521 cgcccggtaa aatggacgat atcttcgata cccagttcgg cgacgagttt cttaacgaca
    14581 ccgtttttct tcggctctcc gattaccgtc agtttcacag gctgtttttt tctgatccgg
    14641 taaacggctt ccagcagata gcgcagccct ttcaaaggcg tatccgcgct gttggtaaca
    14701 ataatggagt tttggggacg cgatccgttc tgaaccggat aaaaaaactc tttgttgatc
    14761 ccgttgggca ccacgcgaaa cttggacgac ggaatggaaa attcctcagc aatgtctatt
    14821 ttggaacatt ccgaaaccgt cacgatatgc gaaagctggc gggcgacttc tttctgcatc
    14881 cggatgaagg aataccattt tttgatctta ttaattttcc ttttgctggt cgccgacttg
    14941 aactcttcct gacggtcgac ggtgatggga tgatgaatcg tcgctatcgt gggaacgacg
    15001 ttctgcgcta ttttaccgat cccgtaagca agacactggt tatcgtggac gatatcatac
    15061 ttcttccggt gtcgttgcag gtaattataa gcccggacgc cgaaagtata cggttcggga
    15121 aaccggcccg tacacatggt cagaaattcg tacatattca gcggattcat cagggcgcgc
    15181 gcgttgcgca cccgaaaggg atgttcgaca ttataaagat ccaggctggg aagtctgtgc
    15241 agacgcaccg gtggatccag atgcggataa ggcggtcccg acaacacatc gacctcatgc
    15301 cccagatcac tgagtgcctt actcaaatat ttgatgtata ctccctgccc gccacaattg
    15361 ggattgcccc ggtaggtcag cagacaaatt ttcaacggct gtgatttgga tttacctttc
    15421 atactgcaac ttttttacca tccttcgcga attttcaaac atagcggata cttctcttgc
    15481 attcagtccg gcgccgcttg caatgcgttg cgctctttcc cggccggcaa aatcagccgg
    15541 cgcgtgggca tcattattga gcatcagctt cgctttgcat ttccttgccg tctgcgccac
    15601 atgcccgttg gtgaaactgt gacctctccg cgttgtgatt tcgagataaa ttccccgttc
    15661 ggcggcaagc cgcgcttctt tttccgtcag gagtccggga tgcgccagga tatcaatgtc
    15721 tgctttcagg gcagccagat ttgttccggc aggcaccggc tccacaatcg tttccccgtg
    15781 tacaaccacc accttcgccc ccagttttct ggcttcattc gccaaaccgg cgatctgagc
    15841 gggcgcaaca tgcgtgattt cgactccggg gaaaacgttg atctttcccg cctggttaat
    15901 tttgaaacag gccctgatga tccggggaat gatgaaatcg atattggaat gatccgcgtg
    15961 atcggtaatg gccagaaact tataacccgc ggcccaggct cgctgcgcca gttctgcggg
    16021 tatcaactcg ccatcactga aaatggaatg agtatgcaga tcaatcactt aaaaaatcct
    16081 gattctaaat atacgacaga tttagaatac ttaatctgat aattactagc aaatttagga
    16141 ccgggagtca ataaggatgt gcagaccgct aagcattgaa ctacttggat ttttccttta
    16201 acagcagaaa cgttgccgaa acgcggcaga catttttgtc tttattgtcg gtaaccagac
    16261 agtctccgat catgaggctt ttcccctttt taaaaacggt tgccgtcgcc gtgagttccc
    16321 cgccgctgac gccggccaga aaattgcttt tcagttcgat ggtggttaag ccttcctcag
    16381 gagttaactg cgtcatcatc gcatgagcga tcgtctcatc cgccagcgca accatcaggc
    16441 ctccctgcat ggctttggcc ccctgcagat actccgggcg aaccggcatt ttaaaac
//
LOCUS       DAOSME010000050        16447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4205_length_16447_cov_22.943875, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000050 DAOSME010000000
VERSION     DAOSME010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16447
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_16447_cov_22.943875"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1833)
                     /locus_tag="PLG98_08075"
     CDS             complement(256..1833)
                     /locus_tag="PLG98_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE33251.1"
                     /translation="MNKTAKLIEIDLNPEFRRALELMEETDKNIFITGRAGTGKSTLL
                     NYFYHYTNKNVVLLAPTGVAAVNIGGQTIHSFFQFKPNVTLAAIKKKKGADKDKKSLY
                     KKLTTIIIDEVSMMRADLLDCVDKFLRLNGPEFKRPFGGVQMIFIGDLYQLPPVVTAA
                     EREIFRSHYRTPYFFSAKVMDELDMEFVELEKVYRQKDDEFIRLLNAIRNKSITDGDI
                     ALFNQRCDPSFEMSADDFYISLTSTNDLADSINEQRLAQLSGKVWKAQGFVEGEFGAE
                     SMPTAVELKLKKGAQIMLLNNDAYGQWVNGTIGKVKKFAKNEDGDDVIVARLDNGETV
                     EISPYTWKIYKYFLKDEELHSEGVGSFRQYPVRLAFAVTIHKSQGKTFERVVVDVGRG
                     TFAHGQMYVALSRCTTLDGLVLKNPLKKSHVLMDWQVMKFLTGLQYAKAELNCTREQK
                     IAMLEEAFHEKQVVEIVYLKAKDEKSTRKIRPLRVEEMEYNGHPYLGLYAYCLMRRQN
                     RVFNVDRILKIRLPSND"
     gene            2072..2818
                     /locus_tag="PLG98_08080"
     CDS             2072..2818
                     /locus_tag="PLG98_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE33252.1"
                     /translation="MPIHMDTRNEVAILAMDNSPENAFTSDMIQQFNATLDRIEADDK
                     IRGVVVTGADGQFFSSGMDLQYVMKLEVKAIKIFFKTLFTFFHRTFVFPKPMVGAING
                     HVVASALAFSMCMDYRVMQKEKAVCTFPEIDVSITPPSGCLAMVKYVIGERMTDLAFL
                     SGRKFDGAAALAAGMVDELAESRSVIDRAVEVATELGAKKPSLFAEYKKRFRGETADR
                     MLAEDMRYIDKEMDVTMFQNCDLSCLRSLP"
     gene            2845..3579
                     /locus_tag="PLG98_08085"
     CDS             2845..3579
                     /locus_tag="PLG98_08085"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33253.1"
                     /translation="MSAADYGKFLNRPVPELPENILNKLKNNPNISKIAFADANQIIH
                     DENLQNEIGFAELNNKEYLVAMSTELPNTDSKMIDWWFWWQAQESERYQLWFPGEHKS
                     ISYKKSQRAYFSNKYQAFLENTQYPVERVGKITVELSIRFVDPVRFGIKDKEKVLKHN
                     GTIICGYVGILKGIIQHTKMLHYFKPNKKGLILISRFWLGNGLPRVIRGIAANKDQAF
                     AMAKHCWIEYTRLGQILPEIYHQAKT"
     gene            4175..4948
                     /locus_tag="PLG98_08090"
     CDS             4175..4948
                     /locus_tag="PLG98_08090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33254.1"
                     /translation="MSGDNKSIHEFDFKLICEYFSSRERQGPGSPEVTVKALSFIDNL
                     THDSKIADIGCGTGGQTMVLARHTPGHITGMDLFADFIDIFNANSRKLNLQDRVQGIV
                     GSMEHLPFHNEELNLIWSEGAIYNIGFERGINEWRSFLKQGGFLAVSEASWFTEKRPA
                     EIDRYWKDAYPEIDVISHKVGQMQKAGYIPVTTFVLPENCWTEHFYAPQVLAQEKFLK
                     KYAGNKTAEDLVANERHEAMLYNKYKAFYGYVFYIGKKI"
     gene            5054..5569
                     /locus_tag="PLG98_08095"
     CDS             5054..5569
                     /locus_tag="PLG98_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE33255.1"
                     /translation="MMDKFKIRICHHEDIGILTETIRKSFRDVAQRFGLTRRNAPSHP
                     SNCMLNWIRYDMERGVTYFIAENESRTAGCVACEQASAKICYLERLAVLPEERNQGLG
                     KALVERVLSNARELGAGTVSIGIIADHAELKNWYRKIGFVEGETRTFINLPFLVTFMS
                     YNLNENSLREK"
     gene            complement(5624..6244)
                     /locus_tag="PLG98_08100"
     CDS             complement(5624..6244)
                     /locus_tag="PLG98_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOE33256.1"
                     /translation="MKKIVQYAAMITLAVLFAVYPFQPASAQVGLYLGGWGGYTIHPN
                     ATEDDWDYYDDVDLDFDETSAWGAKVGYTNPFFKYLALEAEYLYLNPEVEDDYVLDGD
                     VKMSNLMFNIIGKYPLGIIQPYFGLGLGFSYADTSWDGIRGIDDGDYTSYAWQIMTGL
                     EIFLTSEIAVDVGYRYFATEMEFDHEDSDDVTFSTGMVTLGLKVFF"
     gene            complement(6332..6901)
                     /locus_tag="PLG98_08105"
     CDS             complement(6332..6901)
                     /locus_tag="PLG98_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOE33257.1"
                     /translation="MKVLGIMGSPRIGGNSDILLDDALAGAEEAGARVEKIVLAKKKI
                     SGCLDCKKCNDTGLCVVKDDMPAIHKKILEADAIIHSVPVYFWLMTAQMKAYLDRWCA
                     LFDATWSWQKSYYPKMKGKRIGLITVCGDPNVHTADPIVHSFKSTSDMTKMRWMGAVM
                     ASANDKGEIVKDEKARKKAFKLGKKAATP"
     gene            7035..7850
                     /locus_tag="PLG98_08110"
     CDS             7035..7850
                     /locus_tag="PLG98_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOE33258.1"
                     /translation="MPESFHFFSYHRREQDDFTVIAHRGASAYYPENTMPSFEGAIVM
                     GADMAELDVQLTADQEVVVFHDEKISRCTNGRGRIADYDLAYLKQLDAGKWFDKKFKN
                     TRIPELADVLALCKNRIAVNIEIKTEAVDDIIAGGIEEKCLQLVEQYGMKKHVVFSSF
                     DPRAILHLKQIDKTAPVAVLFEKKLYGSRLPSDIVKSLHADAFNCSASELNKKWLADL
                     HSNNIPVNIYTVNNEKKMRQYLKLGVSGIFTNKPDVLKNVLADGDQKSKKTNL"
     gene            7847..>8393
                     /locus_tag="PLG98_08115"
     CDS             7847..>8393
                     /locus_tag="PLG98_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOE33259.1"
                     /translation="MNISKDLVNFIMFSLDTLIQTAGIDIRLHGLEKVPDQPVVYVIN
                     HFTRLETVLMPYLIKKHLHKEAVSLAHESFFSGKMKDFMDNVGAVSTADPHKDKIFIH
                     ALLSDRHPVIIFPEGQIIKDKKIVEKGKYIVYNTGIRRPPHSGAARIALLTEFIREKL
                     RTLKARNDMASIARIASRFDFS"
     assembly_gap    8394..8493
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8494..10182
                     /locus_tag="PLG98_08120"
     CDS             <8494..10182
                     /locus_tag="PLG98_08120"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE33260.1"
                     /translation="ARNDMASIARIASRFDFSGNDLEKIIRKETYIVPVNITYYPVRA
                     RENALSRLAGKFFKNMSARLQEELEVEGPIVLGKVDIDINFGRPIAIKRYIHSKWKMK
                     RMMADGNVYLNTGDFKRISAFRQVCVRLMYDYMNAIYGMTTVNHDHIMSYILTSYPQG
                     SFSENDFKKRIFLAIEHLRKIALPNCHTSLYKKQFYLLTDDQHEKYESFMNEAVTNNY
                     LRRENGVITKNKERFSVPSDFHTIRRDNIIEVLRNEIKPLRHFTRIMEWQKFIPAFFI
                     KWNVRNTFLKLEKELFHQDYEQFYLPGESKPKNIGEPFFLKRFFSRKGIILVHGYMAA
                     PEEIRPLADYLHQNGYNVYGVRLRGHGTAPEDLAIRNWEKWYDSASRAYIIMKNSVKT
                     FSIAGFSMGGGIALLQAANKPGRFASVISINAPMVLRGMASKFAPAVVAWNTLLKKIN
                     IDKWKMEFIKNDPENPDINYLRNPVSGSYELEKLMKRVDHHLKNIADPVLIIQASNDP
                     VVDPCSGRDIFNKIGGGNKQFFMVNANHHGILRGKEATEVHAKVLAFLSESASK"
     gene            10273..10620
                     /locus_tag="PLG98_08125"
     CDS             10273..10620
                     /locus_tag="PLG98_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOE33261.1"
                     /translation="MIEQWMTEIKRTAPADMLGMILSHNGIVRATAKTGESVQAMHLS
                     YDKTKLEKAVREFKGKEGIVDIRVWINEGHLKIGDDIMNVCVAGRFRKDVLPVFQELI
                     SMIKTEIVKEEEI"
     gene            complement(10718..11329)
                     /locus_tag="PLG98_08130"
     CDS             complement(10718..11329)
                     /locus_tag="PLG98_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33262.1"
                     /translation="MIRHAVVMVSAICFWGAAFLTVPAYADYRIILKNGETISVSNYE
                     MDGDNIVFNKYGGLVSFSRSGIAKIEEDNIEEDIPEENMAEDDANEADDSEQPAKNKV
                     SKKSGQKSEDPKNDREKKIAECQRILKMKKDSMDIYCWQAASAKVSAAPNLPQTNQAR
                     VSEKTGKQFQDHVQSNVDAFKANSTCDYYKRKVAEQEKLCNGL"
     gene            complement(11433..12626)
                     /locus_tag="PLG98_08135"
     CDS             complement(11433..12626)
                     /locus_tag="PLG98_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33263.1"
                     /translation="MLTSDLDTTRLAIIGPGRLGTAFAYKFGRDNKRVAIYYHDAEVC
                     KAINRDHLNPLHLTEDLAGRLGGMEKVPRLADKVYATNDLEHIVENNDFIILTITMNR
                     LPEFLNYLKPMIDKKAGDTCLISPIKGLISDEISKELITPSQLINNSLYNLKHKYQIG
                     CIGGPFFDMDIALGNPVCLTVAGKKRIANIIREDLLKFNRKEINSYYNFDIVGVEACG
                     ALKNVVANIKGLADSLQLGDSIPGTIFARSGVEIRSLSRLMGGSFQAFHSQAGVGDMY
                     VTVSSLASKNYRYGKYFYELYTGNPIETNLRVLEKIDGTPEGPNTVRNVHKYLEKKNM
                     YSPLFSCAYHIFNEAGEKGAIRDLIIQACQYDKRNKEYIDAFSRLMYRILPDFWYRRD
                     KSWFD"
     gene            13138..13803
                     /locus_tag="PLG98_08140"
     CDS             13138..13803
                     /locus_tag="PLG98_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOE33264.1"
                     /translation="MEKQTLGKEITQEFHDGKIKEKKYVRQGKTEGEYFSFYHSGQIL
                     AHVNFIRGKKNGPAVIYYHDGHIREKATFVNDRMEGEYVSYFENGHIREHEFYRNGKL
                     DGKYEIYYKTGQLKLVETFRNGKFEGEYIAYYKNGQIKEKGFFKNDKREGEYIAYYKS
                     GQIREKATYKNGNCVGKFLAFEEEISGSGETNGSESDTEIWDEKDVANLLEELKHFDK
                     KQK"
     gene            13830..14681
                     /locus_tag="PLG98_08145"
     CDS             13830..14681
                     /locus_tag="PLG98_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE33265.1"
                     /translation="MISNILIPTDGSANSQTALDYGIYIARKLAASLTGLYVIDVNLI
                     QGPMVTDISGSIGMPPYEGFFESIEKSLNEKADFILKEFEERCRKSGVKAATDRKTGK
                     ISPIIIEEAQKADLILMAKKGEHFHLKEGGLLGSVAESVTRNSGKPVLVTPEKFVEIE
                     SMALAFDGSESATKALGLSLNLSHKTSWPLTVVIVSPDEKKAEALSGKVDKMIQQNPD
                     EASIDCETIILSGKEQDEIVKFIREGSVELMVMGAYGHNRLRELLLGSTTSHVIHKSP
                     IPVLLIR"
     gene            14736..15935
                     /locus_tag="PLG98_08150"
     CDS             14736..15935
                     /locus_tag="PLG98_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE33266.1"
                     /translation="MEHRYGKNILPLSTFDNENFIGSPPLEILFFYKKDLPILKLKES
                     LLKTIHYYNLFSSRLIMIGDHKFALLYCTDGAVANDLRPVDACFDDSRIAEIRKEMVH
                     VQTLPGEPLFAVTGIPFKNGILAGISCSHAVADGISLVLFLYAWMCITDGKNFLPPSP
                     QRLFQGPLAHFDPTDRALVPPLSALNDQIREKVDIDNVAEIFTATEYFTDDFLNAIKA
                     EAEKENYDISNHQIMTAFLLKKYHRKILPDTDRIVLKNPINLRDIHPDIDPMYIGSSN
                     FNNFIQFTKEEIDRLSISQIASRIKKSIADVRNESYAREIAHLSPYGIEINMDVFRRN
                     RPPYSVGTNVVSSNLTHLNDLESMFVGPETGSLLHIGLAVQTGFTMLKEKSGRIFAQI
                     TGRHPLT"
     gene            complement(16002..>16447)
                     /locus_tag="PLG98_08155"
     CDS             complement(16002..>16447)
                     /locus_tag="PLG98_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOE33267.1"
                     /translation="TVSAATEAAIMGFPSMAVSLDTHKEADFTFAAKFARKMTRMILN
                     HREQLLGSAINVNVPCLPQGKIKGVVVVRQAQTNIVEYFEKRTDPRENTYYWFAGESS
                     SATRQKDTDVGALTSGFITVTPIHFDLTRHDLLDQLKDALEKNRF"
BASE COUNT         4632 a         3821 c         3677 g         4217 t
ORIGIN      
        1 cggcgacgtc cagcttggga agctgacatt ctaccgctga attacgcccg ctcatgaagg
       61 cgtaatgtta tttaaccgga gcattttgtc aaggtgtttt taatgacacc cgctgatagt
      121 tatatccttt gagagcgcat gaagagatgc tcgaaaagta agttgaacta taccagccgc
      181 gtaacaccat ggtaaaacaa gccccgattg atcgggatga atttcgtccc tgccgcctga
      241 tggcggcggc attattcaat catttgacgg caaacgaatt ttcagaatgc ggtccacgtt
      301 aaaaacccgg ttttgcctcc gcataaggca ataagcatac aaccccagat atggatgacc
      361 gttatattcc atttcctcca cacgaagcgg gcgaatttta cgcgtcgatt tttcatcttt
      421 cgcttttaaa taaacgattt cgacaacctg cttttcgtga aacgcctcct ccagcatcgc
      481 aatcttttgc tcgcgggtac agttcaactc cgctttggcg tactgcaaac ccgtcagaaa
      541 tttcatcacc tgccaatcca ttaaaacatg acttttcttt aacggatttt ttaacacgag
      601 accgtccagc gttgtgcagc gggatagcgc cacatacatc tgcccgtggg caaacgtgcc
      661 gcgacccacg tcaacaacca ctctttcaaa tgttttcccc tggctcttgt gaatcgtcac
      721 tgcaaaagcc aggcgcaccg gatactggcg gaaagaaccg accccttccg aatgcagttc
      781 ctcatccttg agaaaatatt tataaatttt ccacgtatag ggagaaattt ccaccgtttc
      841 accgttgtcc agtctggcga caatgacatc gtcgccatct tcatttttgg cgaatttttt
      901 tactttaccg atggttccgt taacccactg accgtacgca tcgttattga gcaacataat
      961 ctgcgcaccc tttttcagct tcaattcgac agcggtgggc atggattctg ctccgaactc
     1021 tccttcaaca aatccctgcg ccttccagac tttaccggac agttgcgcca gccgctgttc
     1081 attaatgctg tccgccagat cattggtgct ggtcaatgaa atataaaaat catcggcgga
     1141 catttcaaag gacggatcgc agcgctgatt gaacagcgca atatccccat ccgtaatgga
     1201 tttattacga atggcgttga gcagacggat aaattcgtca tccttctgac ggtaaacttt
     1261 ttccagttcg acaaattcca tgtccagttc atccatcact ttcgcgctaa aaaaatacgg
     1321 ggttctgtaa tgcgaccgga aaatttcgcg ttccgctgct gttaccaccg gcggcaactg
     1381 ataaagatcg ccgatgaaaa tcatctggac accgccgaaa ggccgtttga actccgggcc
     1441 gtttaaacgc agaaacttat ccacacaatc gagcagatcg gcccgcatca tggaaacttc
     1501 atcgatgatg attgtcgtca attttttata aagcgatttt ttgtccttgt cggcaccctt
     1561 ctttttttta atggcagcca acgtaacatt cggtttgaac tggaaaaagg aatggatggt
     1621 ttgtccgccg atattcacgg cggcaacgcc cgtgggcgcc agcaaaacaa catttttatt
     1681 tgtgtaatga taaaaataat tgagcagtgt ggatttgccg gtaccggcgc gtcccgtaat
     1741 gaaaatattt ttatccgtct cttccatcag ctccagcgcc cgccggaact cgggattcaa
     1801 atctatttcg attagctttg ccgttttatt cattatatat ttaacatttt tcactgtaaa
     1861 atgttaatag tgtttatcac aaaagattcg gtggcaaaag ttcatttctt tagatctctg
     1921 ataacttggg atattcttca tcaagaggtt tttaatcttc ttgacacata aaatcgcgtt
     1981 ttttatactt catcaataat aaaggacgct ctagccaact caattaattt attttttaaa
     2041 acgtatcgat ttttgacaaa ggaggctgac tatgccgatt catatggata ccagaaacga
     2101 ggtggcgatt ctggccatgg acaacagccc ggaaaacgcc tttaccagcg acatgattca
     2161 gcagtttaac gcgacattgg accgcattga ggccgatgac aaaattcgcg gcgtggtggt
     2221 gaccggagcc gacggccaat tcttttcttc cggcatggac cttcaatacg tcatgaaact
     2281 ggaggtaaaa gccattaaga tatttttcaa gacgcttttt acttttttcc acaggacctt
     2341 tgtgtttccc aaacccatgg tcggtgccat caacggccat gtggtggcca gcgccctggc
     2401 tttctctatg tgcatggatt accgggtgat gcaaaaggaa aaggcggtgt gtacctttcc
     2461 ggaaatcgat gtttccatta ctccgccttc cggatgcctg gctatggtga aatacgtcat
     2521 cggcgagcgc atgaccgatc tggccttttt gtccggccgc aagttcgatg gagctgcggc
     2581 tctggccgca ggcatggtgg acgaactggc cgaaagccga tcagtcatcg accgggccgt
     2641 ggaagtcgcg acggaattgg gcgctaaaaa accgagcctg tttgccgaat ataaaaaacg
     2701 cttccgcggt gaaaccgcag accgcatgct ggccgaagac atgcgatata tcgacaaaga
     2761 gatggacgtg accatgtttc agaactgcga cctttcctgc ctgcggtctc taccataatc
     2821 cgcattaact aaatggagat ttgtgtgagt gctgctgact atgggaaatt tttgaataga
     2881 cctgttcctg aattacctga gaatattctc aataagctga agaataatcc caatatttca
     2941 aaaattgcat tcgctgatgc caatcagatt attcatgacg agaatctaca aaacgaaatt
     3001 ggttttgcag aacttaacaa caaggaatac ctggtggcca tgagcactga attgccaaat
     3061 acagattcaa agatgatcga ttggtggttc tggtggcaag ctcaagagag tgaaagatat
     3121 caactgtggt ttcccggtga gcataaatca ataagctata aaaaatcgca acgcgcatat
     3181 ttcagcaata aatatcaagc gttcttggaa aacacacaat atccagtcga aagagttgga
     3241 aagattactg ttgagttatc catcaggttc gttgatccgg taagattcgg aataaaagat
     3301 aaagaaaaag ttttaaaaca taatggaaca atcatatgtg gttatgtagg aatattaaaa
     3361 ggaattatac aacatacaaa aatgcttcat tattttaaac caaataaaaa agggctgata
     3421 ctcattagcc gattttggct aggaaatgga ttgcccaggg taattagagg cattgcagcc
     3481 aataaagatc aagcatttgc aatggcaaaa cattgttgga ttgaatatac aaggctgggg
     3541 cagatacttc ctgaaatata ccatcaggcg aagacataac cattcaatcc gccagaccga
     3601 aagcagcgcg ttggcatgct tccgcacttg ccctgcatat tgccagacta ttacgatatg
     3661 caccacaatg cttgcggctg gtgatcgcca tcacttggca cgaaagaata caggaaggat
     3721 accatgattg cattttgcgg attggattgt ttacaatgcg aggcgtatct tgcaacgcga
     3781 gaaaacagcg acaatcaacg atacgacgtg gcaaaaaagt ggtcagctct ttatcataaa
     3841 gatattaagc caggccagat aaactgcgac gggtgcaaat caaacggccg cttgtttttt
     3901 cattgtaaaa acacgtgcgg gattcggaaa tgctgtaaat caaaaggggt tgacaactgc
     3961 gcggtatgca gtgactatgc gtgtgcaatc atggcagagc tcaccacatt agctccagag
     4021 gcaggtaagg cccttgaaaa actgagatag tagtaaatgc gaaccgggta tgggaggcat
     4081 aacgggccgc cggcacaaaa tgcctcaacg tgtcatttga tgaactgtca tgcctgacgg
     4141 aagaggaaat gctggaaaat aaaaaggaga cgcaatgagt ggggacaata aatcaattca
     4201 tgagtttgat tttaaattaa tctgtgaata tttttcaagc agggaacgac aagggcccgg
     4261 gagccccgaa gtcacggtca aagcgttaag cttcattgat aacctgactc acgactccaa
     4321 aattgccgac atcggttgcg ggacaggcgg ccagacaatg gtgctggccc ggcatacgcc
     4381 agggcatatt acaggcatgg atttgtttgc cgacttcatc gatattttca atgccaacag
     4441 ccggaagctc aatctccagg atcgcgtgca aggcattgtc ggctccatgg agcatcttcc
     4501 ttttcacaac gaggaattga atttaatctg gtcggaaggt gcgatctata atatcggatt
     4561 tgaacgaggc atcaacgagt ggcggagttt cctgaagcaa ggaggctttc ttgccgtttc
     4621 agaggcttcc tggtttaccg aaaagcggcc tgccgaaatc gaccgctatt ggaaagatgc
     4681 atatcctgaa atcgacgtca tctcccataa agtcgggcag atgcaaaaag ccggatatat
     4741 tccggtgaca acttttgttc tgcctgaaaa ttgctggacg gaacattttt acgctccgca
     4801 ggttcttgca caggagaaat tcctgaaaaa gtatgcgggc aataaaacgg ctgaagacct
     4861 cgtggcaaac gagcggcacg aagcaatgct ctataataaa tataaagcat tttatggata
     4921 tgttttctat attggaaaaa aaatttaatt cacaagatca agcatccgtt gtgttatcgg
     4981 ataatggacc ggttgtttca ccggatcagc cgaagcacgg cgttgaatgc ttcacaacac
     5041 cggcaaatta taaatgatgg ataaattcaa gattcgtatt tgccaccatg aagacatcgg
     5101 aatattgacg gaaaccatcc ggaaatcatt tcgcgatgta gcacagcgtt tcggcctgac
     5161 tcgccggaac gctcccagtc atccgtccaa ttgtatgctc aactggatca gatacgatat
     5221 ggaacgcggc gttacgtatt tcattgcgga aaacgaaagt agaacggccg gttgtgtggc
     5281 ctgcgagcag gccagcgcaa aaatttgcta tcttgagcgc ctggctgtgc ttcccgaaga
     5341 aaggaaccag ggcctgggaa aggccctggt tgaacgtgtt ttgtcaaacg cccgggaact
     5401 gggcgcgggt acagtcagca ttggcattat tgccgaccat gccgaattga aaaactggta
     5461 tagaaaaatc gggtttgttg aaggcgaaac caggacgttt atcaacctgc cgttccttgt
     5521 cacctttatg tcttataatc tgaacgaaaa cagtctccgt gaaaagtaaa tagaaataga
     5581 cttttcagaa catcctcaga caattcccgg aaacgaacag attttagaaa aataccttca
     5641 gccccagcgt taccattccc gtggaaaacg tgacgtcgtc ggaatcctca tggtcaaatt
     5701 ccatctcggt ggcaaaataa cgataaccga catccacagc aatttcactg gttaagaata
     5761 tttccagtcc ggtcatgatc tgccaggcat aagacgtgta atccccgtca tcaattcccc
     5821 ggataccgtc ccaggacgta tccgcatagg aaaatcccaa ccccagccca aaatagggtt
     5881 gaatgattcc gaggggatat ttcccgatga tgttgaacat caggttgctc atcttgacat
     5941 cgccgtcaag cacatagtca tcttctactt cagggtttaa atagaggtat tcggcttcca
     6001 gggcaagata cttaaaaaaa ggattggtat aaccgacctt ggcgccccag gcggacgttt
     6061 cgtcaaaatc cagatcgaca tcatcataat aatcccagtc atcttctgtt gcattagggt
     6121 gaattgtata gcctccccat ccaccaagat ataatccaac ctgagccgaa gccggttgaa
     6181 aaggatagac agcaaacaaa acggccaggg taatcatggc agcatattga acaatttttt
     6241 tcacagtgat tctccttttc gcaaattatt ttgttgtatt ctaatgtttt atattccgct
     6301 tttcaatatg tttttcagaa taaaacaggc atcagggtgt tgcagctttt ttgcccagct
     6361 taaaagcttt ttttctggct ttttcatctt tgacgatttc ccctttatcg ttggcagagg
     6421 ccataactgc acccatccag cgcatttttg tcatatccga agttgattta aagctgtgga
     6481 cgattggatc agccgtgtga acattcggat ctccgcagac ggtaatcaaa ccgatgcgct
     6541 ttcctttcat tttcggataa taagatttct gccagctcca ggtcgcgtca aacagcgcgc
     6601 accaccggtc cagatacgct ttcatctgcg cggtcatcaa ccagaaataa accggcacgc
     6661 tgtgaataat agcatccgcc tccagaatct ttttgtgaat ggccggcata tcatccttga
     6721 cgacgcagag gccggtgtcg ttgcattttt tgcaatccag acatccggat attttctttt
     6781 tggccagaac aatcttttcc acccgagcgc ccgcctcttc ggcgccggcc agggcgtcgt
     6841 cgagcagaat atcgctgttg ccgccgatcc gcggacttcc cataatgccg agaactttca
     6901 tagagtcatc ctccttgttc ataagaattc tattcaagac gcccgctcat ttatgtatca
     6961 taaatccttt cggtatggtt tgtaaacact aaaataattg aagaactctg ctcttaaata
     7021 tggtaaaaaa aaacatgcct gaatcatttc attttttcag ctatcaccgg cgcgaacagg
     7081 atgattttac cgtcatcgcc catcgcggag cttcggctta ttatccggaa aacaccatgc
     7141 cctcctttga aggcgccatc gtcatgggtg cggacatggc ggaactggac gttcagctca
     7201 ccgcggatca ggaagtcgtt gtgtttcacg acgaaaagat ttcacgctgc acaaacggcc
     7261 ggggaagaat cgccgattac gatcttgcgt atttaaaaca gttggacgcc ggcaaatggt
     7321 ttgataaaaa gtttaaaaac acgcgcatcc ccgaactggc cgacgtgctg gcactctgca
     7381 aaaaccgcat cgccgtaaat attgaaataa aaacggaagc ggtggacgac atcatcgccg
     7441 gcggcattga agaaaaatgt ctgcaactgg tcgagcagta cggcatgaaa aagcacgtcg
     7501 tattttccag ttttgatccg cgcgcgatcc tgcatctaaa acagatcgac aaaacggctc
     7561 cggtggctgt tttatttgaa aagaagcttt acggttcccg cctgccttcg gatattgtca
     7621 aatcgttgca tgccgatgca tttaattgct ccgcatccga attgaataaa aaatggctgg
     7681 cagacctcca ctcgaacaat attccggtaa atatttatac ggtcaacaac gaaaaaaaga
     7741 tgagacaata tttaaagctg ggcgtcagcg gaatttttac caacaaaccg gatgttttaa
     7801 aaaacgtcct ggcggatgga gatcagaaaa gcaaaaaaac aaatctatga acatatcaaa
     7861 agacctggtc aattttatca tgttctcgct ggacaccctg attcagacag cagggatcga
     7921 catccgtctg cacggcctgg agaaagtccc cgatcagccg gttgtctatg tgatcaatca
     7981 ttttacgcgt ctggagacgg tcttgatgcc gtatctaatc aaaaagcatc tccacaaaga
     8041 agccgtttcc ctggcgcatg agtctttttt cagcggtaaa atgaaagatt ttatggataa
     8101 cgtgggcgct gtttccaccg ccgaccccca taaagataaa atattcattc atgcgcttct
     8161 ttccgacaga catcccgtaa tcatttttcc ggaaggacag atcattaagg ataaaaagat
     8221 cgtcgagaaa ggaaaatata ttgtttacaa caccggcatc cgccgtcccc cccattccgg
     8281 tgcggcgcgc atcgctctgc tgaccgagtt tatccgcgaa aagttgcgaa cgctgaaagc
     8341 gcgtaacgat atggcatcca tcgcccgcat cgcatcgcgc tttgattttt cagnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgcgta acgatatggc atccatcgcc
     8521 cgcatcgcat cgcgctttga tttttcagga aatgatctgg agaaaattat tcgcaaagaa
     8581 acatatatcg ttccggtgaa cattacctat tatcccgtcc gcgcccggga aaacgccctg
     8641 agccggctgg ccggaaaatt tttcaaaaac atgtcagccc gcctgcagga ggaactagag
     8701 gtggaaggcc ccatcgtgct gggcaaggtg gatatcgaca tcaacttcgg caggcccatc
     8761 gcaatcaaac gttatatcca ttcaaaatgg aaaatgaaaa gaatgatggc ggatggaaat
     8821 gtttatctga atacaggtga tttcaaaaga atatccgcat tccgtcaagt atgcgtcaga
     8881 ttgatgtacg attacatgaa tgccatatac ggcatgacca ccgttaatca cgaccacatc
     8941 atgtcttata ttctgacgag ctaccctcag ggcagcttca gcgaaaacga ctttaaaaaa
     9001 cgtattttcc tggccattga acatttgagg aaaatcgccc tgcccaactg ccatacatcg
     9061 ctttacaaaa agcaatttta cctgctgacc gacgatcagc acgaaaagta tgagagtttc
     9121 atgaacgaag ccgtcaccaa caactatctc cgaagggaaa acggcgtcat tacaaaaaac
     9181 aaagaacggt tcagcgtgcc atccgacttt cataccatcc gcagagacaa cattatcgag
     9241 gtgctccgaa atgaaatcaa accgctgcgt catttcacca ggatcatgga atggcagaaa
     9301 ttcattccgg cattttttat taaatggaac gtcagaaaca cgtttctcaa actcgagaag
     9361 gaactttttc atcaggatta tgaacagttc tatttaccgg gtgaaagcaa accgaaaaat
     9421 atcggcgagc cttttttcct gaaacgtttt ttcagccgca aaggaattat tctggtgcac
     9481 ggttatatgg cggcgccgga agaaatcagg ccgctggcgg actatctgca tcagaatggc
     9541 tataacgttt acggcgtgcg cctcaggggc cacggtacgg cgcccgagga tctcgccatc
     9601 cggaactggg agaaatggta cgactccgca agccgcgcat acatcatcat gaaaaatagc
     9661 gtcaaaacgt tttcaatcgc cgggttttcc atgggcggcg gcattgcgct tttacaggcg
     9721 gccaataaac cgggccggtt tgcatcggtc atatcgatca acgccccgat ggtattaaga
     9781 gggatggcgt caaaattcgc gccggccgtc gtcgcctgga atacactgct caaaaaaatc
     9841 aacattgaca agtggaagat ggaattcatt aaaaatgatc cggaaaatcc ggacattaat
     9901 tacctccgca atcccgtcag cggcagttat gaactggaga aactgatgaa aagggttgat
     9961 catcatttga aaaatatagc cgacccggtg ctgatcatcc aggcctcgaa cgatccggtt
    10021 gtggatccct gcagcggccg ggatattttc aataaaatcg gcggcgggaa caaacagttt
    10081 ttcatggtca acgctaacca tcatggtata ctgcgcggca aagaggcgac cgaagtccat
    10141 gccaaggtcc tggctttttt aagtgaatcc gcttcaaaat aatttattta tcccggcgaa
    10201 ttcacgaaag tgaacgtggt actctctaca ggagttgccc gtaaaaacag caaaatcatt
    10261 aaggggaata ttatgattga acaatggatg acagaaataa aaagaacggc tccggccgat
    10321 atgctgggga tgattctttc ccacaacggc attgtccggg cgacagcaaa aaccggcgaa
    10381 tcggttcagg cgatgcacct ttcttatgat aagacaaagc tggaaaaagc ggtgcgcgaa
    10441 tttaaaggaa aggaaggcat cgtcgatatc cgggtttgga tcaatgaagg tcacctgaag
    10501 atcggcgacg acattatgaa tgtttgcgtg gccgggcgct tccgcaaaga cgttttgcct
    10561 gtttttcagg aactgatttc catgattaaa acggaaatcg ttaaagaaga agagatttaa
    10621 caaaaagttg atctggaatg gcgccgtgca ccgcggccgg ttcaagccac ggatggggtg
    10681 atgattcaat agaaccattg ccatttattg ggcgatgtca tagaccgttg catagcttct
    10741 cttgttcggc aacctttctt ttgtagtaat cacacgtcga attggcctta aaggcatcga
    10801 cattactttg tacgtgatcc tggaactgct tgcccgtttt ctccgatact ctcgcctgat
    10861 tcgtctgagg aagattcggg gcagcgctga cttttgcaga agcagcctgc cagcaataaa
    10921 tatccatact gtcctttttc atcttcagaa ttctctggca ttccgcaatc tttttctccc
    10981 gatcattctt cgggtcttcg cttttctgcc cggatttttt tgacacttta ttctttgccg
    11041 gctgttcaga atcatcggcc tcattcgcat catcctctgc catgttttct tccggaatat
    11101 cctcttcgat attatcctct tcgattttag cgattcccga acggctgaaa ctgaccaggc
    11161 ccccatattt gttaaaaacg atattatcgc catccatttc gtagttgctg acggaaatgg
    11221 tttctccgtt tttcaatata atccggtagt cggcgtacgc cggaacagtc aaaaaagcgg
    11281 ctccccaaaa acaaattgca gaaaccatga cgacggcatg tctgatcatt cgcgatgtct
    11341 cccgtaaaat gaaaggaatg ctgtgctgat ttttatctgt cctcttcctt tatttttaat
    11401 attattttgc aaacagtgtt taatgatccc ttttagtcaa accaactttt gtcgcggcga
    11461 taccagaaat ccggaagaat acggtacatt agccgggaaa aagcatcgat gtattctttg
    11521 tttctcttgt catactgaca ggcctggatg atcaaatctc ttatcgcgcc tttttcaccg
    11581 gcttcattaa atatgtgata cgcgcaggag aaaagcgggc tgtacatatt cttcttttcc
    11641 agatatttgt gcacgtttct gacggtattg gggccttcgg gcgttccatc gattttctcc
    11701 agaacgcgca gattggtttc gatgggattc ccggtataaa gttcataaaa atactttcca
    11761 taacggtaat tcttgctcgc cagcgaggaa accgttacat acatgtcccc cacgcccgcc
    11821 tgactgtgaa aagcctgaaa gcttcctccc atcaggcggg acaacgaacg aatctccaca
    11881 ccggaccggg caaagatggt tccggggatg gagtccccaa gttgcaggga atccgccagt
    11941 cccttgatat tggccacgac gtttttcaaa gcaccgcagg cttccacgcc gacaatgtca
    12001 aagttgtagt aggaattgat ttccttgcgg ttgaatttca gcaaatcttc tctgatgata
    12061 ttggcgatgc gcttcttgcc ggcaaccgtc agacacaccg gattgcccag cgcgatatcc
    12121 atgtcaaaaa acggtccgcc gatgcatccg atctgatatt tgtgcttcag gttatacaag
    12181 ctgttattga tcagttgcga cggcgtgatc agttctttgg aaatttcatc ggaaatcaat
    12241 cctttaatcg gagaaatcag gcaggtatcg cccgcttttt tatcaatcat cggcttgaga
    12301 taattgagaa attccggcaa gcggttcatc gtgattgtca gaataataaa atcgttattt
    12361 tcaacaatat gttccagatc atttgtggca tacaccttgt ccgcaagacg gggaaccttc
    12421 tccattccgc ccagacggcc ggccagatct tctgtcaaat gcagaggatt taaatgatcc
    12481 cggtttatcg ccttgcagac ttccgcatca tggtaataaa tggcaacccg tttattatca
    12541 cgaccgaatt tataggcaaa agctgtgccc agacggccgg gaccgataat agcaagcctt
    12601 gtggtatcta aatcagacgt cagcatcaat caatctcgtt gtgaaaaaat tcgtctttat
    12661 ggcaatgcaa aacacattac cggtttttaa tatcttcttt taaaaaaaaa ttaaactaaa
    12721 tttattaatg agcactgatt ccctacccat gcgtttcggg gaaatcaaga gtattttcga
    12781 tattctccgc taatgcggga tagttcaact aataaacctt gctgcgctct gcctgcaatg
    12841 cttgagtttc actaattcga ccaacgactc aaacgttctt cactattcat tagcccgctc
    12901 cgctgcgctt cggggataat tcgactaacg tttcgaacgt cgttgcactc gttctcagcg
    12961 agtctcatta tttttgttgc attatcataa atgattgttt aaaaaaagca atgggttgat
    13021 ttcagaagca tggcagagga ccacgaagca acacggcaag aatattttcg taaaacgatc
    13081 ttcagaattt taaacttgca ttcatccaat tgaaggcaaa aaagagatta tattttaatg
    13141 gaaaaacaaa cacttggtaa agaaatcacg caggaatttc acgacgggaa aattaaagaa
    13201 aagaaatatg tgcggcaagg caaaaccgaa ggcgaatatt tttctttcta ccatagtggc
    13261 caaattctgg cccatgtcaa tttcatccgc gggaaaaaaa acggccccgc ggtcatttat
    13321 tatcacgacg ggcatatcag agagaaggca acctttgtca acgaccggat ggaaggcgaa
    13381 tacgtttctt acttcgaaaa cggccacatc cgggagcatg agttttacag aaacggcaag
    13441 ctggatggaa aatatgagat ttattataaa accggccagt taaagctggt cgaaactttt
    13501 agaaacggca agtttgaagg tgaatatatc gcctattaca aaaacggaca aatcaaggaa
    13561 aaaggatttt ttaaaaacga caaacgggaa ggtgaatata tcgcctacta caaaagcgga
    13621 caaatccggg aaaaagccac ttacaaaaac ggcaactgcg tgggaaaatt tctggccttt
    13681 gaggaagaaa tatccggttc cggggaaaca aacggcagcg agagtgacac cgaaatctgg
    13741 gatgaaaaag atgttgccaa tttgctggaa gagctgaaac attttgataa aaaacaaaag
    13801 tgaatgtttt atcgccagga ggcgtcatta tgatcagtaa cattttaatt cctacggacg
    13861 gttccgcaaa cagccagacg gctttggatt acggaatcta tattgcccgt aaacttgccg
    13921 cctctctgac aggcctttat gtcatcgatg tcaatctgat tcaggggcct atggtgacgg
    13981 atatttccgg ttcgatcggc atgccgccct atgaaggatt cttcgaatcc atagaaaaat
    14041 cgctcaatga aaaagccgat ttcattctga aggaatttga ggaacgctgc cggaaatccg
    14101 gtgttaaggc cgccacggac aggaaaaccg gcaaaatcag cccgattatt atcgaagaag
    14161 ctcaaaaggc cgatctgatt ctgatggcaa aaaagggaga acattttcat ctgaaggaag
    14221 gcggtctgct ggggtcggtt gccgagtcgg taacacgcaa ttccggaaag ccggtattgg
    14281 ttactccgga aaaatttgta gaaatagaaa gcatggcact ggcttttgac ggcagtgagt
    14341 cggcaacaaa agccctgggt ctttctttga atttatccca caaaacctcc tggccactga
    14401 cggtggtgat tgtcagccca gacgagaaaa aagccgaggc gttatccgga aaggtcgata
    14461 aaatgattca gcagaatccg gatgaggcct ctattgactg cgaaacgatc atcctgtccg
    14521 ggaaagaaca ggatgaaatc gtcaaattca tccgggaagg ctctgtggaa ttaatggtca
    14581 tgggggctta cggtcacaat cgtctgcgcg aattgctgct cggcagtacc acatcgcacg
    14641 ttatccacaa aagtccgatt cccgtattgt tgatccggta acctgaagtt tgttcaatgt
    14701 ccgagtcgac aataccggca attgaataga aaaaaatgga acatcgttac ggcaaaaaca
    14761 tccttccgct gtcgacattc gataatgaaa atttcatcgg gtcacccccc ctggaaattt
    14821 tattttttta taaaaaagat cttcccatat taaagctcaa agagagtctg ttaaagacga
    14881 tccattatta caatcttttt tccagtcgac tgatcatgat cggcgatcat aaatttgcgc
    14941 ttctgtattg cacggatgga gccgttgcaa atgatctgcg gcctgtcgac gcctgttttg
    15001 atgacagccg cattgccgag atcaggaaag aaatggtgca tgttcaaacc ttgccgggcg
    15061 aacctctctt tgccgtaacc ggcattcctt tcaaaaacgg gatactggcc ggcatcagtt
    15121 gctctcacgc ggtggcggat ggtatttccc tggtgctttt tctttacgcc tggatgtgca
    15181 taacggacgg aaaaaatttt cttccaccgt cgccccagag attgtttcaa ggcccgctcg
    15241 cccatttcga tccaacagac agagcgcttg ttcccccctt atccgcgctg aacgatcaaa
    15301 tccgggaaaa agtagacatc gataacgtcg cagagatttt caccgccaca gaatacttta
    15361 ccgatgattt tctcaatgca attaaagcgg aagcagaaaa agaaaattac gacatttcca
    15421 accatcagat catgacggcg tttttattaa aaaaatatca ccgtaaaatc ctgccggaca
    15481 ccgaccggat cgttttaaaa aatcccatca atctcagaga catccatccg gatattgatc
    15541 cgatgtatat cggcagttca aatttcaaca acttcataca gttcaccaaa gaagaaatcg
    15601 acaggctttc gatatctcaa atcgcgtctc gtattaaaaa atcaatcgct gatgtgcgca
    15661 atgaaagtta tgcgcgggaa atagcacatt tatccccgta cggaattgaa atcaatatgg
    15721 atgtcttcag aagaaaccgc cctccctaca gcgtgggaac gaatgtcgtc tccagcaacc
    15781 tgacgcatct gaacgatctt gaatccatgt ttgtgggacc agaaaccgga agcctgttgc
    15841 atatcggcct ggcagtccaa accggattta ccatgctgaa agaaaagagc gggcggatct
    15901 ttgctcagat aaccggcagg catccattaa cctgatgccg ataaccttta cgggcggtga
    15961 cgcgatgtcc gtcatcgctg gctcggcaat cagccaatgt cttaaaacct gtttttctcc
    16021 agggcatctt ttaactgatc gagcaggtca tgtctggtca aatcaaaatg gatcggtgtg
    16081 actgtaatga atccggacgt cagcgcgccg acgtcggtat ctttctgccg ggtggcggaa
    16141 gacgactccc cggcaaacca gtaataggta ttttcccgcg ggtccgtccg tttttcgaaa
    16201 tactccacga tattggtctg cgcctgacgg acaaccacaa cgcccttgat ctttccctgg
    16261 ggaaggcagg gcacgttgac attaatcgcg ctgcccagaa gttgttcccg gtgattgaga
    16321 atcatgcggg tcattttacg cgcaaatttg gcggcaaacg taaaatccgc ttctttgtgc
    16381 gtgtccagag aaaccgccat ggacgggaaa cccataatcg ccgcttcggt tgccgcggaa
    16441 accgttc
//
LOCUS       DAOSME010000051        16153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4295_length_16153_cov_24.700025, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000051 DAOSME010000000
VERSION     DAOSME010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16153
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4295_length_16153_cov_24.700025"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLG98_08160"
     CDS             complement(<1..483)
                     /locus_tag="PLG98_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33268.1"
                     /translation="MRKNIITKILLSRKLYQLSLENLNSDNELSLSIGVILLQDSVEI
                     FLLAVAEHIDASIGNNSNFNQYFDLINKKLSPKELPFKLRLTALNKLRVNAKHYGLSP
                     SKLETHGLIETVKEFFEEVSIQVFEKEFVTISLVDLINKGEAKDYLMAAENDFSKGDY
                     V"
     gene            complement(670..2832)
                     /locus_tag="PLG98_08165"
     CDS             complement(670..2832)
                     /locus_tag="PLG98_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOE33269.1"
                     /translation="MSENPLHLLMNPQSIAVAGANNNPSKMGTIQALNIVKGGYGGRF
                     YPIHPVEKTVLGCPAYATPEDLPEAPDLAILIVPIRAVASLLEGFGKIGTKRAIVITA
                     GFKETGAAGRDMEKKINEIADRYQMRFVGPNCMGVINSGISLNTTVLATSREPGLLGF
                     ASQSGTFLSQTLPYLNKRGIRFSKAISLGNEANINMIDALEYLGEDEQTKAIILYIEG
                     IREGRRFLDVARNITPHKPIAALYVGGSASGARAGLSHTGAMAGPDFLYNGIFKQAGI
                     IRVNSIEDLYYHGWTLATQPPMRGKRVGVMTNSGGPSTTISYTCDAVGLEVPRFSDGL
                     QNEIRKHIEPHASASNPVDMTFDLSMNKLALTLPEMVMKSGEVDAVVLHGTMMTGYLK
                     EVYPTLKDIIGNIPLDDFLKYGQMDRTIANETFELPSKYNMPMLISSFFDHEDNYIKG
                     YQDTNTPVFYSPENTARALGSLYLYKQIKERAPRKEAALPKIKKEATEIILKALDNKQ
                     KALDEYEAKQLLACYGVPVTKEALAAKVEDALKTAKKIGYPVALKACSWKIMHKSGKG
                     LIALNVENENQLKKEFENIQKNAGKTAPVLVQEMLSGSREFLAGMTRFAGFGPGVVFG
                     LGGVFTEVFKDTTMRLAPVTSADALEMFEDINARKLLDKFRGLPKVKLSNLSRIIQAL
                     GNIALLHPQIAEIDLNPIIINGAEPVVADALIVLEDKS"
     gene            complement(2832..3434)
                     /locus_tag="PLG98_08170"
     CDS             complement(2832..3434)
                     /locus_tag="PLG98_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE33270.1"
                     /translation="MPPKVIYSRSDIIDSSLRIVKEKGFKQLSAREVARQLISSTRPV
                     YEHFQSMDELKKAVLQKTVDLLYDYVTRQYTKNAFLNTGVGYVLYARDHKEFFKMIFL
                     EDNDASGIIDEMLKKLDKEIVKVPELKKLAPAERKDLLKNGWIYTHGCAVMVFAGYIK
                     NNQDKQIIKMLAEAGPIFIEDALRKHNADALINTTIKEIE"
     gene            3692..5173
                     /gene="lysS"
                     /locus_tag="PLG98_08175"
     CDS             3692..5173
                     /gene="lysS"
                     /locus_tag="PLG98_08175"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOE33271.1"
                     /translation="MEDNELLNIRREKIADLKSAGIDLYPNDIKPKNTTQEITLKFGD
                     MDNDSLAKQEETFSVAGRLMAVRNFGKAAFIKIQDYKGQIQGYIGKNSLSPEEYFVFK
                     KLDIGDIIFISGKLFRTKTNELTIEAQKLMLLSKSIRPLPEKWHGLTDIETRYRQRYL
                     DLIANPKVKEIFHRRSLIIKLIRQFMDEHDFLEVETPMMQPRAGGAAARPFKTHHNAL
                     DMDFYLRIAPELYLKRLVTGGLERVYEINRNFRNEGISTFHNPEFTMMEFYQSYATYE
                     DLMAFTEDLFCFLAKNIFGGLKFNYQDTEIDLTPPWRRISVKDALLQLANMDAAALDD
                     PALALSFARKVGCKVNDNDPLGKILMAIFDEVVEKKLIQPTFVTHYPVVISPLSRRSK
                     NDPDLTDRFELYIYGREIANAFSELNDPVDQKGRFLQQLHEREAGDDEAHEMDEDYVR
                     ALEYAMPPTAGEGIGIDRLVMLFTDSSSIRDVILFPLLRTRQE"
     gene            5235..6518
                     /locus_tag="PLG98_08180"
     CDS             5235..6518
                     /locus_tag="PLG98_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOE33272.1"
                     /translation="MPYELFISRRYLKAKRKQVFVSVITFISVLGIFLGVAALIIVLA
                     VMNGFEEDLRTKILGIKSHVELTTDMSGPMKNYRDVREKIAGIEGVVASTPFIYTQAM
                     IRNDNGVTGVVIRALDTESAFRVINLGKIKEGNLDHLNKIPQDIAAGYSDNGVQPDGI
                     VIGKEMARNMGIFLYDTITIISPVSIATPAGMVPRMGKFVVVGIFESGFYEYDSTLAY
                     LSLPSCQKFLQMGDTVTGIDIKVNDIYKADVIARKIQSALGFPYWTQNWMQMNKNLFS
                     ALKLEKRVMFIILSLIVLVAAFNIISALIMIVMEKNKDIAILKSMGATSASIMKIFVY
                     QGLIIGVIGTFLGCAAGLAVALNLQKVSLFVEKIFNFKFLPGDVYYLSELPSKVNYSD
                     VAIIVAGTLLICFLSTIYPSLRASKTDPAEALRYE"
     gene            6543..7214
                     /locus_tag="PLG98_08185"
     CDS             6543..7214
                     /locus_tag="PLG98_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE33273.1"
                     /translation="MIVLENLSKTFFKAGNKIEVLRGLNFKIEKGQSLAVLGASGAGK
                     STLIHIMGMLDHPTGGLLLLDGRNVFAWEEKKRAAFRNSHIGFVFQFNNLLPEFNALE
                     NTMMPALIGGFNKKQAGEKACKLLQEVGLESRITHKPGELSGGEQQRVAIARALIMEP
                     DILLVDEPTGNLDTETGKKIEDILVNLNVTKKITLVTVTHNKLLAERMSERILLRDGE
                     IYEYQ"
     gene            7201..9846
                     /gene="bamA"
                     /locus_tag="PLG98_08190"
     CDS             7201..9846
                     /gene="bamA"
                     /locus_tag="PLG98_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOE33274.1"
                     /translation="MNTSKKSFKISLALLLFVFLFVPPAYSEDLKKIVVMPFEVFSNQ
                     DKTVIRESFYKNLQAELQKEKSIQIIAADDFLQREGQIDENTALDHGKAKGADFIVTG
                     TLTQFGKSMNIDCKIFDVEKQSSLPAVSTHGRGMASTTALAEQLKVEILDRIGIVQKI
                     ARIEIQGNRKINASAITTKLKSRKGGIFSEADIAADIKTIFKMGYFLDVTAETDATPE
                     GKVITFFVQEKGLVSEIVINGNKAVSKDDIQEVLTIKTRQSLNREQIKADIEKIKALY
                     DSKGYYNAEIKDRVEQDGETDFRVVLEIRENDRLYIRSIAFEGNEAFPAKELKKLMST
                     SEKGFFSFITDSGLLKRDQLKQDIGKINAYYFNNGFINAQIGEPETTADQKGIHVKIK
                     IQEGKRFKVDNVAISGDVLEKPREALLQSLKVKKGDNYNREAIIRDLDLLMQACNDEG
                     YANAEINPKIDTRENEQLVDVDFQIAKGEPVYISNINISGNSITRDKVIRRQLDVVEG
                     DLYSSSKLRSSYANLSRLRYFEEVDFQTEKGTDKNKMDINVRVKEKSTGMFMVGAGYS
                     ASDKLVMMAQITQQNFLGYGQVLSLKASLGSETSNYDLSFTEPWLFDLPLWCKADIWK
                     YEKEYDYYTIDTQGAGLTLGYPLIERIVGYLGYKYYIDDIREVSPIATDYVKAQEGET
                     SSSAVTLSLIRDTTNDYIFPSKGTKATVSVTHAGGPLEGDANYTQYGASLFAYFPLPL
                     DVVFAAKGRIGYIQDHEGVEIPVSNRYVLGGINSLRGFRYVGPTNGGTSDVIGGTNMM
                     VFNVELVLPFIKESGMKLVAFYDAGNSWDNKYDMGDLRQSVGAGIRWYSPIGPLRLEY
                     GHVLDRGELNDDSKGRWEFTIGMPM"
     gene            9895..10413
                     /locus_tag="PLG98_08195"
     CDS             9895..10413
                     /locus_tag="PLG98_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOE33275.1"
                     /translation="MKKFFYLTAIMTMLVFAWSANVLAADKIGFINLQEIMQTSNAGK
                     KAADEFKRFYEKETQEIKSMENQLKKMKDELEKQGSVLTQSSRSEKEAAYQKKMRDYQ
                     LLVNDTNEELKKRDQEMTQKLMPGIIKIVRSIAEREKYTLVIDVATMPIPYYAKENDF
                     SNKVIEEFNKTK"
     gene            10432..11454
                     /gene="lpxD"
                     /locus_tag="PLG98_08200"
     CDS             10432..11454
                     /gene="lpxD"
                     /locus_tag="PLG98_08200"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOE33276.1"
                     /translation="MQMTTGEIAAFLGGTVMGADDVVIGHIRAIDEAGPGDLTFIANK
                     KYIKKLKTTKASAILVSPQTTAEGKNLIVVSDPYVALGKILSHFYPAQHGRSGVSAEA
                     CIEDGADVSPDAVIFPRAFISAGAKIGAGTVIYPGVFIGRNASIGEHSIIHANVTVYH
                     SCIIGNRVILHSGAVIGADGFGFAAPGRDNVKIPQVGFVQIDDDVEVGANTTIDRATL
                     GRTWIQRNVKIDNLVQIAHNVVIGENSVITAQVGISGSTQLGKGVMVGGQTGMVGHIT
                     IGDHVMIAAGAGIHKDIKSGDMVGGAPQLPYKEWLQVEACRAKLPEMRATLNDLVKQV
                     EELKKK"
     gene            11478..12251
                     /gene="lpxA"
                     /locus_tag="PLG98_08205"
     CDS             11478..12251
                     /gene="lpxA"
                     /locus_tag="PLG98_08205"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOE33277.1"
                     /translation="MKIHETAIIAPGAQLEEGVEIGPYSIIGADVRIGKNTVIGPHTV
                     IDDYTQIGESCRIFQFCSIGAPPQDLKFSGEKTRVVIGNFNTIREFVTIHRSTTADIG
                     VTIMGNHNLIMAYCHVAHNCKLGNHVIMSNAATLAGHVHVEDYAIISGLTGVHQFCRV
                     GAHCMIGGCSAAVKDIPPYTIAQGNHAKLYGLNLIGLKRRNFPEKTIKAISDAYRIVF
                     RSKLLLEDAIKKAESEVEDLPEVRHFIKFIKESERGICR"
     gene            12262..13215
                     /locus_tag="PLG98_08210"
     CDS             12262..13215
                     /locus_tag="PLG98_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE33278.1"
                     /translation="MKHKKIKVGVIGIGHLGNYHLQKYRKLEPCEIVAVADTSSERAQ
                     KAAQIYQCAAFSDYREMIGSVDAVSIAVPTSDHYNVAREFLAAGVDVLIEKPLCATLA
                     EADDLIGLARKKKLILQVGFVERFNPAIMALENVIRKPLFIESHRLHPFFERGTDVDI
                     ILDLMIHDLDILVKFVDAPAISVEAMGVPILSDKIDIANARITFSTGCTANITASRIS
                     AKTMQKIRFFSPEGYHSVDCQKREVLSFSKGTTGSGQMQIVQNHLEIGSHDPLEEEIR
                     SFVDAVITRGCPPVSGEEARKSLALAVDIITKMKAAEDLLT"
     gene            13212..14306
                     /locus_tag="PLG98_08215"
     CDS             13212..14306
                     /locus_tag="PLG98_08215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE33279.1"
                     /translation="MIPMVDLKRQYHSLKRDIDAAISNVLEDTRFILGPNVSALEEEV
                     AAYHGPSQAVGVANGTDALLLALRASGIEAGDEVITTPFTFIATAEVVALLKARPVFV
                     DIEPVTFNMDWRQIEGKITSRTKAIIPVHLFGHPADMDPIMAIAKKHNLRVIEDCAQA
                     FGAVYHGRKVGAIGDIGCFSFFPSKNLAGYGDGGMIVTRSDETAAKIKMLRNHGSARR
                     YYHDEVGYNSRLDEIQAAIIRIKLKKIDEFNAARRRNAALYCAAIKRKDIVLPSASGD
                     CEHVYHQFTIRSDERDLLADALQKKEVASAVYYPVPLHRQNAFLNLYDGPETMPQSET
                     CAREVLSLPMFPELDQKEIEFIADIINKAS"
     gene            14327..15475
                     /gene="lpxB"
                     /locus_tag="PLG98_08220"
     CDS             14327..15475
                     /gene="lpxB"
                     /locus_tag="PLG98_08220"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOE33280.1"
                     /translation="MKDSNHKNKTVLIIAGEASGDMHGANLVREMLNRDPALKFYGIG
                     GKKMLQEGVQLLADASTMAVVGLTEVVFKLKDILKIMRTIKKSLDERRPDLVVLIDYP
                     DFNLPVAKAARRHNIKVLYYISPQVWAWRQSRIEQIKKTVTRMAVILPFEVETYRRHG
                     FAADYVGHPLLDMIKTTYSKKESRKKFDLHEDKITIGILPGSRMSEIKKLLPELLQAA
                     TIMKDSIPELQFVVPLADTLEEKNLTGIIAGFPIDVNVISGQTYDVVSCADLALVASG
                     TATLETALLKVPMIIVYKISPLSYFIGKLFVRVKNIGLVNIIAEKTIVPELIQNDASG
                     ARIAREALSILGDETKKQAMIRELEAIRSRLGEPGAAIKTAKLALDML"
     gene            15492..>16153
                     /locus_tag="PLG98_08225"
     CDS             15492..>16153
                     /locus_tag="PLG98_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOE33281.1"
                     /translation="MKIFKRLLSLGRPYSVRFLISMLCMVIAGALTSSQAVLVKPVLD
                     DIFLAKNPASLKWLPLAVVMIFLVKGICNYVQTVLMNFIGLRIVTDLRNQLYEQIQKQ
                     SLSFFTHHPTGLLMSRITNDVASIQNAVSDVVTSIVKDSFTIVALICVIFYTDWKLAF
                     IAMIIFPLTIYPISAFGKKIRKVTTSTQITMANLSSLLQETIAGTRIVKAFGMETHEI
                     KR"
BASE COUNT         4303 a         3912 c         4028 g         3910 t
ORIGIN      
        1 aacataatct ccctttgaga aatcattttc agcagccata agataatctt ttgcctcacc
       61 tttattgatc aaatcaacaa ggctgatggt tacaaattct ttctcgaata cttggataga
      121 aacttcttcg aagaactcct ttacagtttc tataagacca tgtgtttcta atttagaagg
      181 agataaacca tagtgcttag cgttaaccct gagcttgtta agagcggtta ggcgaagctt
      241 aaagggaagt tcttttggag aaagtttttt atttattagg tcgaaatatt gattaaaatt
      301 tgaattatta ccaatactag catctatatg ttcggcaaca gcaagaagaa atatttcaac
      361 agaatcttgt aatagaataa cgccgatgga aagagaaagt tcattatcag aatttaaatt
      421 ttcaagtgat aattgataaa gtttccgaga gagaagtatt tttgtaatta tatttttccg
      481 cattatttac tccttaacgg atttttataa caattcccta tctttcttat gacactctta
      541 atgcaaaagg attgcttaat aattacggat tgtaacctag cataaaatta tatttttgaa
      601 aaatgaggat ttcaggaagc aagacttgac actaaattat taggccaagt ctcctgcgga
      661 gcgataaact taagatttgt cttccagtac gattaaggca tcggcgacca ccggttcggc
      721 gccgttgatg ataatcggat tgagatcgat ctcggcgatt tggggatgca gaagagcgat
      781 gttgcccagc gcctgaatga tcctggacaa attgcttaat ttcaccttcg gcaggccgcg
      841 gaatttatcg agcagtttgc gggcattgat gtcttcgaac atttccagcg catcggcgct
      901 tgtaaccggc gcgaggcgca ttgtcgtgtc cttgaaaact tccgtgaaaa cgccacccag
      961 accgaaaaca acacccggac cgaacccggc aaagcgcgtc ataccggcca aaaattcgcg
     1021 gctgccgctg agcatttcct gcaccagcac cggcgcagtt tttcccgcgt tcttttgaat
     1081 attttcaaat tcttttttga gctggttttc gttttccaca ttcagcgcga tgagtccctt
     1141 gccgcttttg tgcatgattt tccaggagca ggctttgagc gccaccggat agccgatttt
     1201 tttagcggtc ttcagagcgt cttcaacctt tgccgccagc gcttccttcg ttaccggcac
     1261 accgtagcag gcaagcaact gtttcgcctc gtattcatcc agcgcctttt gtttgttgtc
     1321 cagcgccttg aggatgatct ccgtggcctc ttttttgatt ttcggcaaag cggcttcttt
     1381 gcggggagcg cgctctttga tttgtttgta caggtaaagc gagcccagcg ccctcgcggt
     1441 attttccggc gaataaaaca ccggcgtgtt tgtatcctga tagcccttga tatagttgtc
     1501 ttcatgatca aagaaagaag atatcagcat cggcatgtta tatttggatg gcaattcaaa
     1561 ggtttcgttg gcaatcgtcc ggtccatctg gccgtatttc agaaaatcgt ccagaggaat
     1621 attgccgata atatccttca acgtcggata aacctctttc agataaccgg tcatcatggt
     1681 tccgtgcaga acgaccgcat cgacctcgcc gcttttcatc accatctccg gcagggtgag
     1741 cgcgagtttg ttcatgctca ggtcaaacgt catatcgacg ggattggacg cagaggcgtg
     1801 cggttcaatg tgttttctga tttcgttttg cagcccgtcg gaaaagcggg gcacttccag
     1861 accgaccgca tcgcaggtat aggaaatggt tgtggagggc ccgccggaat tggtcatgac
     1921 gccgactctt ttgccgcgca tcggcggttg tgtggcaagc gtccagccgt gatagtaaag
     1981 atcttcaatg gagttgaccc ggatgatgcc ggcctgcttg aaaataccgt tatagaggaa
     2041 atccggaccg gccatggcgc cggtgtgact caaccccgcg cgcgcgccgg aggcggaacc
     2101 gcccacgtat aacgctgcaa tcggtttgtg cggtgtgatg tttcgggcaa cgtcgagaaa
     2161 ccttcgccct tcgcggatgc cttcaatgta cagaatgatg gctttggttt gttcatcttc
     2221 tcccagatat tccagcgcgt caatcatatt gatgttggcc tcgttgccca gactgatggc
     2281 cttactgaaa cgaatccctc ttttgttgag atagggcagc gtctggctca ggaatgtgcc
     2341 gctttgcgac gcaaatccca gaaggcccgg ttccctggac gtggctaaaa cggtggtgtt
     2401 taatgaaatc ccggaattga tgacacccat gcagttggga ccgacaaaac gcatctgata
     2461 gcggtctgct atttcgttga tctttttttc catgtcccgt cccgccgcgc cggtttcctt
     2521 aaacccggcg gtgattacga tggctctctt cgtgccgatt ttgccgaaac cctccagcag
     2581 ggaagcaacc gctcgtatgg gcacaatcag gatggccaga tcgggcgctt cgggcaaatc
     2641 ctccggtgtg gcataggcgg ggcaacccaa aacggttttt tccacgggat gaatcgggta
     2701 aaatctgccg ccgtagccgc ctttcacaat attcaatgcc tgtatagttc ccatcttcga
     2761 cgggttgtta ttcgctccgg caaccgcaat ggattgggga ttcatcagca aatgcaatgg
     2821 attttccgac attattcaat ctcctttatc gttgtattga tgagcgcatc agcattgtgc
     2881 tttcgaagtg catcttcaat aaaaatggga cctgcttcag caagcatctt aataatttgt
     2941 ttatcctgat tgttttttat gtatccggca aaaaccatga cggcacatcc atgggtgtaa
     3001 atccagccgt ttttcaacag atcctttctc tcggccggag caagtttctt gagttccggc
     3061 accttcacga tctctttatc cagcttcttc agcatttcgt cgatgattcc gctggcatcg
     3121 ttatcctcca ggaatatcat tttaaaaaat tctttatgat cccgggcata caaaacatag
     3181 ccgacgccgg tattcaggaa ggcatttttc gtgtactgcc gcgtgacata atcatagaga
     3241 agatcaacgg tcttttgcag caccgccttt ttcaactcgt ccatggattg aaaatgttcg
     3301 tataccggcc gggtggagga aatcaattgc ctggcaactt cgcgggcgct cagttgctta
     3361 aatccctttt ctttgacgat tctcaacgag gaatcgataa tatcggacct gctataaata
     3421 acttttggcg gcatgcaatt cccggtctgt ttaaattaca tgtgtaatgt aacgtgtgtt
     3481 atgtattatg tcaagcgttt attgattatg aaataataaa gaattttgaa tgcaggtctc
     3541 atttatcaac gaacgtcgtt attttcagac cttgcacggc gctcccattg cgattgacgt
     3601 cgcatggagg cctattcccc ttattatcgt tatacttgac tatttggcta aaaaaatata
     3661 gttaagcaga ctttacaaaa gtggagaagc tatggaagat aatgaacttt taaatatccg
     3721 ccgtgaaaaa attgccgatc tgaaatcggc gggcattgat ttatacccca acgacatcaa
     3781 accaaaaaac acaacacagg aaataacgtt gaaattcggc gatatggata acgattccct
     3841 ggcaaaacag gaagagactt tttccgttgc cggacgtctg atggccgtgc gcaatttcgg
     3901 caaagcggct tttatcaaaa ttcaggatta caagggacag atccagggct acatcggcaa
     3961 gaacagttta agcccggaag aatattttgt tttcaagaaa ctcgacatcg gcgacatcat
     4021 ctttatctcg ggtaaattat tccggacaaa aaccaacgag ttgaccattg aagcgcagaa
     4081 gctcatgctg ttatcgaaat cgatacgtcc cttgcccgaa aaatggcatg gcctgaccga
     4141 catcgaaaca cgataccgcc agcgatatct tgatctgatc gccaacccca aggtgaagga
     4201 aatatttcac cggcgtagcc tcattatcaa gttgatccgt cagttcatgg atgagcatga
     4261 ttttctggag gtggaaaccc cgatgatgca gccgcgcgcg ggaggcgccg cggcccggcc
     4321 gtttaaaacg catcataacg ctctggacat ggatttttat ctgcgcattg caccggaact
     4381 ttatttgaaa agactggtga ccggcggcct ggagcgggtc tacgaaatca accgcaattt
     4441 ccgcaatgag ggcatctcca ccttccacaa tccggaattc accatgatgg agttttatca
     4501 gtcctatgcc acgtatgaag atttgatggc ctttaccgaa gacctgttct gttttctggc
     4561 gaagaacata ttcggcggtc tgaaattcaa ttatcaggat acggaaatcg atctgacccc
     4621 tccgtggcgg cgcatttccg tcaaagacgc tctcctgcag ctggcgaaca tggacgcggc
     4681 ggcgctggac gatccggcgc tggctttatc ttttgcccgc aaggttggtt gcaaggtgaa
     4741 cgacaatgat ccgttgggaa aaatcttaat ggctattttt gacgaggtgg tggaaaagaa
     4801 actgatccag ccgacatttg tcacccacta cccggtcgtc atttctccgc tctcccggcg
     4861 atccaaaaat gatcccgatc tcaccgaccg gtttgaactg tacatttacg gtcgagagat
     4921 tgccaatgcg ttttcggaat tgaatgatcc ggtcgatcag aaaggacgat ttctgcagca
     4981 gctccatgag cgggaagccg gcgacgacga agcccacgaa atggacgagg actatgtccg
     5041 cgctctggaa tacgccatgc cgccgaccgc cggagaaggc atcggcatcg accgtctggt
     5101 gatgcttttt accgacagtt cctccatcag agacgttatt ttattcccgc tgttgagaac
     5161 cagacaggaa taaacggatt attgacggcg ttgccgaacc ggacttatcc cggaaaaagg
     5221 atataacaat accgatgccc tacgaacttt tcatcagcag gcgttattta aaggccaaac
     5281 gcaaacaagt ttttgtttcc gtgataacgt ttatctccgt cctggggatt tttctcggcg
     5341 tcgccgccct gatcattgtt ctggcggtaa tgaacgggtt tgaagaggat ttgcgcacaa
     5401 agatcctggg cattaagtct cacgttgagt taaccaccga tatgagcggc cccatgaaaa
     5461 attaccggga cgtccgtgaa aagattgccg gcattgaagg cgttgttgcc tccacgccgt
     5521 ttatttacac ccaggcgatg atccgcaacg acaacggcgt caccggcgtc gtcattcgcg
     5581 cgctggatac cgaaagcgcc tttcgtgtca tcaatctggg aaaaatcaaa gaagggaatc
     5641 ttgatcatct caacaaaatt ccgcaggaca ttgccgccgg ttattcagac aacggcgtcc
     5701 agccggacgg tattgtcatc gggaaggaaa tggcccggaa catggggatt tttctctacg
     5761 acaccataac gatcatttcc ccggtcagca ttgccacgcc ggcagggatg gtgccccgca
     5821 tggggaaatt cgtggtggtg ggaatttttg aatccggatt ttacgaatac gattcaaccc
     5881 tggcgtatct gtctttgccg agctgccaga aatttctgca aatgggagat acggtaacgg
     5941 gaattgatat taaagtgaac gacatttaca aggcggatgt cattgccagg aaaattcaaa
     6001 gcgctctggg attcccctac tggacgcaaa actggatgca gatgaacaag aatctttttt
     6061 cggcgctgaa gctggaaaaa cgggtgatgt tcatcattct gagcctgatt gtgctggtgg
     6121 ccgcctttaa tattatcagc gctttgatca tgatcgtcat ggaaaaaaac aaggatatcg
     6181 ccatattgaa atcgatgggc gcgacctccg caagcatcat gaaaatattc gtttatcagg
     6241 gattgatcat cggtgtgatc ggtacttttc tgggatgtgc agcgggatta gccgtcgcat
     6301 taaacctgca gaaggtttcg ttgtttgtgg aaaagatttt taactttaaa tttttgccgg
     6361 gagacgttta ttatttgagc gaacttccgt ccaaagtgaa ctacagcgat gttgccatta
     6421 tcgtggccgg cacgttgctg atttgctttt tatcgaccat ttatccgtcg ttgcgggcgt
     6481 ccaaaaccga cccggcggaa gctttaagat atgaataata acccgacaga cgacgcaaaa
     6541 cgatgatagt actggaaaat ttatcaaaaa ccttttttaa agccggaaac aaaattgagg
     6601 ttctgcgcgg tctgaatttc aaaattgaaa agggccaatc ccttgccgtt ctgggcgcct
     6661 cgggcgccgg gaaaagcacc ctgatccata tcatgggcat gctcgatcac cccacgggcg
     6721 ggctgctgct tcttgacggc aggaatgttt ttgcctggga ggaaaaaaaa agggcggcct
     6781 tccgtaattc gcacatcgga tttgtgtttc agttcaataa tcttctaccg gaattcaacg
     6841 cgctggaaaa cacgatgatg ccggccctga tcggaggatt caacaagaaa caggccggag
     6901 aaaaagcttg caaattgctt caagaagttg gattagagag cagaatcact cacaagccgg
     6961 gagaactttc ggggggcgaa caacaaaggg tggccattgc ccgcgccctg atcatggagc
     7021 cggacatcct gctcgttgac gaacctaccg gaaatcttga cacggaaaca ggaaaaaaga
     7081 tagaagacat tctggtcaac ctgaatgtga caaaaaaaat aacgcttgtt acggtaacac
     7141 acaacaaatt actggcggaa agaatgtccg aaaggattct tctgcgcgat ggagaaattt
     7201 atgaatacca gtaaaaaaag ctttaaaata tctttggctc ttttattgtt tgtctttctt
     7261 tttgttccgc cggcttattc ggaagatctc aaaaagattg ttgtgatgcc ctttgaagta
     7321 ttcagcaatc aggataaaac ggtcatccgg gaatcttttt acaaaaacct gcaggcagaa
     7381 ctgcagaaag aaaaatccat tcagattatt gccgccgacg actttctgca acgcgagggg
     7441 cagattgacg aaaatacggc cctggatcac ggcaaggcaa aaggcgccga ttttatcgtc
     7501 accggcaccc tgactcaatt cggcaaatcc atgaatatcg actgcaaaat attcgatgtg
     7561 gaaaagcaaa gttcactgcc cgccgtgtcc acgcatggcc gagggatggc cagcacaacc
     7621 gctcttgcgg aacaattaaa agtcgaaatc ctggaccgca tcggcattgt gcagaagatt
     7681 gcccgcattg aaattcaagg caaccggaaa atcaatgcct ccgcgatcac gacaaaactt
     7741 aaaagcagga aaggcggtat tttttccgaa gccgacattg cggcggatat taaaaccatt
     7801 ttcaaaatgg gatattttct ggacgtcacc gctgaaacgg acgccacgcc ggaaggcaaa
     7861 gtcatcacgt tttttgtgca ggaaaaaggg ctggtgtccg aaatcgtcat caacggcaac
     7921 aaggccgtca gcaaagacga cattcaggaa gtcttaacca tcaaaacccg gcagagcctg
     7981 aatcgggaac aaatcaaggc agacattgaa aagatcaaag cgctctacga cagcaagggg
     8041 tattataatg cggagatcaa agaccgggtg gaacaggacg gggaaacaga tttccgtgtc
     8101 gttctggaga tcagggaaaa cgacaggctc tacatccgat cgatcgcgtt tgaagggaat
     8161 gaagcctttc cggccaagga attgaaaaag ttgatgagca cctcggagaa aggattcttt
     8221 tcgttcatca ccgattccgg gttgctcaag cgtgaccagc tcaagcagga tatcggaaaa
     8281 atcaatgcct attattttaa taacggattt attaatgctc aaatcggcga accggaaacc
     8341 acagccgatc agaaaggcat ccacgttaaa attaaaattc aggaaggcaa gaggtttaaa
     8401 gtcgataatg tggccatctc cggcgatgtt ctggaaaagc cgcgcgaggc gctgctgcag
     8461 tccctcaaag tgaagaaagg cgataattac aaccgggaag ccatcatcag ggacctggat
     8521 cttctgatgc aagcctgcaa cgatgaaggc tacgccaacg cggaaatcaa tcccaagatc
     8581 gatacgcggg aaaacgagca attggttgac gttgatttcc agattgccaa gggggaaccg
     8641 gtttatatca gcaacatcaa catttccggg aacagcatca cccgggacaa ggtcatccgt
     8701 cgtcagctgg acgtggtgga aggggatctc tattccagca gtaaactgag aagcagctat
     8761 gccaatctga gcaggctgag atattttgaa gaggtggatt tccagacgga aaagggaacg
     8821 gataaaaaca aaatggatat caatgtccgg gtcaaggaaa aaagcacggg catgttcatg
     8881 gtgggagcgg gttacagcgc atcggataaa ctcgtcatga tggcacagat cacgcaacag
     8941 aattttctcg gctatgggca ggtattgagt ttgaaagctt cgctgggctc cgaaaccagc
     9001 aattacgatt tatcctttac ggaaccatgg cttttcgacc tgccgctgtg gtgcaaggcc
     9061 gatatctgga aatacgagaa ggaatacgat tattacacca tcgacacgca gggcgcgggt
     9121 ctgacgctgg gttatcccct gattgaacgg attgtcggct atctgggcta caagtattat
     9181 atcgacgata tccgggaggt ttcgccgatc gcaacagact acgtcaaagc acaggaaggc
     9241 gaaacatctt ccagtgccgt caccttgtct ttgattcgcg atacgacaaa cgattacatt
     9301 ttcccctcca agggaacgaa ggccacggtt tcggtcaccc acgcgggcgg ccccctagag
     9361 ggagatgcca actacaccca gtacggagca agtctcttcg cttatttccc tctgcccctg
     9421 gacgttgttt tcgcagccaa gggacggatc ggttatattc aggatcatga aggggttgaa
     9481 attcccgttt ccaacagata cgttttgggc ggaatcaatt ctctgcgcgg cttccgctat
     9541 gtcggaccga ccaacggcgg gacgtccgat gtcatcggcg gtaccaatat gatggttttt
     9601 aatgttgaac tggtattgcc ttttatcaag gaatccggca tgaagctggt cgccttttac
     9661 gatgccggca actcgtggga caataagtac gatatgggag atttaaggca atccgtgggc
     9721 gccggaatcc gctggtactc accgatcggc ccgctgcggc tcgaatacgg tcacgttctt
     9781 gaccggggag agttaaacga cgattccaaa ggacgctggg aatttaccat cggcatgccg
     9841 atgtaaaagc atagataaaa aattaaacat ataatttaaa tgaggagacc acaaatgaaa
     9901 aaattttttt acctgacggc gattatgaca atgctggttt ttgcatggag tgcaaacgtt
     9961 ttagctgctg ataaaatcgg attcatcaat ctgcaggaaa tcatgcagac gtccaatgcg
    10021 ggcaaaaaag ccgccgacga attcaagcga ttttacgaga aagaaacaca ggaaatcaaa
    10081 agcatggaaa accagctgaa aaaaatgaaa gatgagctgg aaaaacaggg ttccgttctg
    10141 acccagagct cacgcagcga aaaggaagcc gcttaccaga agaagatgcg ggattatcag
    10201 ttgctggtca atgacactaa tgaggaatta aaaaaacgcg atcaggaaat gacccagaag
    10261 ctgatgcccg gtattattaa aatcgtccgc tccattgccg aacgggaaaa gtacacgctg
    10321 gtgattgacg tggcgaccat gccgattcct tactatgcca aggaaaacga tttcagcaat
    10381 aaagtgatcg aagaattcaa caagaccaaa taacaccggc acgttcaacc gatgcaaatg
    10441 acgactggag aaatagccgc gtttcttggc ggcactgtta tgggcgcgga tgacgttgtc
    10501 atcggccata tccgggctat cgatgaggcc ggtccgggcg acctgacgtt tattgccaat
    10561 aagaaataca taaaaaaact gaaaacgaca aaagcgtccg cgattctcgt ttctccgcag
    10621 acaacggcgg aggggaaaaa cctgattgtc gtttccgatc cttatgtggc cctgggaaaa
    10681 atactgagcc atttttatcc ggcgcaacac ggccggtccg gggtcagcgc agaggcctgc
    10741 attgaagacg gcgccgatgt ttctccggac gccgttattt ttccccgtgc ttttatttca
    10801 gccggagcca aaatcggtgc gggaacggtt atttaccccg gcgtctttat cggcagaaac
    10861 gcatccatcg gcgaacattc catcatccat gccaacgtca ccgtttacca ttcctgcatc
    10921 atcggcaacc gggtcattct ccattccggg gcggtgattg gcgccgacgg cttcggcttt
    10981 gcagcgccgg gacgcgacaa cgttaaaatc ccgcaggttg gctttgtgca aatcgacgac
    11041 gatgtggagg tgggagccaa cacgaccatt gacagagcga ctctgggcag gacctggatt
    11101 caacgcaacg tcaaaatcga caatctggtc caaattgccc ataatgtggt catcggtgaa
    11161 aattcggtca tcacagccca ggtcggcatt tccggaagca cgcagctggg gaaaggcgtg
    11221 atggtcggcg gacagaccgg tatggtcggc catataacca tcggcgacca tgtcatgatt
    11281 gcggccgggg cggggattca caaagacatc aaatccggcg acatggtggg cggcgcaccg
    11341 cagttgccgt ataaggaatg gctgcaggtg gaagcctgcc gcgccaaact gcctgaaatg
    11401 agagcgacgc tgaacgatct tgtgaagcag gtggaagagc tcaagaaaaa gtaaattctc
    11461 ctaaagaagg ttaactgatg aaaattcacg aaacagctat tattgcaccc ggcgctcaac
    11521 tggaagaagg cgtggaaatc ggcccctact cgatcatcgg cgccgatgtc aggataggga
    11581 aaaatacggt gatcggtccg catacggtca tcgacgatta tacccaaatc ggcgaaagct
    11641 gccggatctt tcagttttgc tccatcggag cgcctccgca ggatctgaaa ttcagcggag
    11701 agaaaacccg ggtggtgatc ggcaatttca acacgatccg cgaatttgtg accattcatc
    11761 gctcgacaac agccgacatc ggcgtcacca tcatggggaa tcataatctg attatggcct
    11821 attgccacgt ggcgcacaat tgcaagctcg gcaatcacgt gattatgtcc aatgcggcaa
    11881 cgttggccgg ccatgttcat gtcgaagatt acgccattat cagcggactg accggcgtgc
    11941 accagttctg ccgcgtcggg gcgcactgca tgatcggcgg atgctcggcc gccgtaaagg
    12001 atattccccc gtatacgatt gctcagggca atcatgccaa actttacgga ttgaacctta
    12061 tcggattgaa aagaagaaat tttccggaaa aaaccatcaa ggccatcagt gatgcctacc
    12121 gcattgtttt tcgttccaag ctgctcctgg aagacgcgat caaaaaagcg gaatctgaag
    12181 ttgaggatct gcccgaagta aggcatttca taaaatttat taaagaatcg gaaagaggca
    12241 tttgccgttg atttacacaa catgaagcat aaaaaaatta aagtcggtgt catcggaatc
    12301 ggccacctgg gcaattatca tttgcaaaaa taccggaaac tggaaccctg cgaaatcgtc
    12361 gccgttgccg acacgtcttc ggagcgcgct caaaaagcag cccagattta tcagtgcgcg
    12421 gccttcagcg actaccggga aatgatcggc agcgtggatg ccgtcagcat tgccgtgccg
    12481 acaagcgacc attacaacgt cgcgagggag tttcttgcgg cgggcgtgga tgtcctcatc
    12541 gaaaaaccgc tttgtgcaac gcttgcggaa gcggatgatc tgatcggtct ggcgcggaaa
    12601 aagaaactca ttctgcaggt cgggtttgtt gaacgattca atcccgccat catggcgctg
    12661 gaaaacgtca tccggaaacc tctttttatc gaatcccata gattacaccc gttcttcgaa
    12721 cgcggcacgg atgtggacat catcctggat ctgatgattc acgatctgga tatcctcgta
    12781 aaattcgtgg atgctccggc gatcagcgtg gaggccatgg gggttcccat tctctcggat
    12841 aaaattgaca ttgccaacgc ccggattact ttttccaccg gctgcaccgc caatattacc
    12901 gctagtcgga tcagcgccaa gaccatgcaa aaaatacggt ttttcagtcc agaaggctat
    12961 cattcggttg attgccaaaa acgggaagta ctgtccttca gtaaagggac aaccggctcg
    13021 ggccagatgc agattgtcca aaaccatctt gaaataggca gccacgatcc gctggaagag
    13081 gaaatccgct cttttgttga cgccgtcatc acccgcggct gcccgccggt ttccggggaa
    13141 gaggcgcgca aatccctggc gctcgccgtg gacatcatca caaaaatgaa agcagcagag
    13201 gatttactga catgataccc atggtcgact taaaaagaca gtaccacagc ctcaaaaggg
    13261 acattgatgc cgccatcagc aatgttctgg aagatacccg ctttattctc gggcccaacg
    13321 tgtccgcgct ggaagaagaa gtcgccgcct atcacggtcc gtcccaggcg gtgggcgtgg
    13381 ccaacggaac ggatgccctg cttctggctt tgcgggccag cggcattgaa gccggtgacg
    13441 aggtcattac cacgcccttt acctttatcg caacggcaga ggtcgtcgct cttttgaaag
    13501 cccggccggt ttttgtggat attgagcccg tcacgttcaa catggattgg cgtcaaatcg
    13561 aaggcaagat aacctcgcgg acgaaagcca ttatcccggt tcatctgttc ggccatcccg
    13621 cggatatgga tcccattatg gccatcgcta aaaaacataa tctccgggtc atcgaagact
    13681 gtgctcaggc atttggagcg gtctatcacg gccggaaagt gggggcgatc ggcgatatcg
    13741 gttgtttcag ctttttcccc agcaagaatc tcgccggtta cggcgacggc ggcatgatcg
    13801 tcacccgaag cgacgagacg gctgcaaaga taaaaatgct gcggaatcac ggcagcgcca
    13861 gacgttacta ccatgatgag gtcggctata acagccgtct ggacgagatc caggccgcca
    13921 tcatccggat caaactgaaa aaaatcgacg aattcaacgc ggcccgccgg cgcaatgcgg
    13981 cgctttattg cgcagccatc aaacgcaagg acatcgtttt gccgtccgct tccggcgatt
    14041 gcgaacatgt ctatcatcag tttaccattc gctcggacga gcgggatctt ctggccgacg
    14101 cgctgcagaa aaaagaggtc gcttccgccg tgtattatcc tgtgccgctt caccggcaga
    14161 acgcatttct caacctgtac gacggaccgg aaacgatgcc gcaatccgaa acatgcgccc
    14221 gggaagttct ttccctgccg atgttccctg aattggatca gaaagaaatc gaattcattg
    14281 ccgatatcat taacaaggca tcctgaagaa aaatccggct gagatcatga aagattcaaa
    14341 tcataaaaat aaaaccgtgt tgatcatcgc cggcgaagcc tccggcgaca tgcacggcgc
    14401 caatcttgtg cgggaaatgc tgaatcgcga tccggcgctg aaattttacg gcatcggcgg
    14461 gaaaaagatg ctgcaggaag gggttcagct tcttgccgac gcctccacga tggcggttgt
    14521 cggcctgacc gaggtcgttt tcaaattaaa agacattctg aaaatcatga ggacgataaa
    14581 aaaatccctc gatgaacggc gtccggatct tgtcgtcctg attgattatc cggattttaa
    14641 tctgcccgtg gccaaagcag cccgtcggca caacatcaag gttttatatt acatcagccc
    14701 gcaggtctgg gcctggcgtc agagcagaat cgaacagatc aaaaagaccg tcacccggat
    14761 ggcggtcatt ctgccttttg aagtggaaac ataccggcgg cacggttttg cggcagatta
    14821 tgtggggcat cccctactgg atatgattaa gacaacctat tcaaaaaaag aatcgagaaa
    14881 aaagttcgat ctgcacgaag ataagatcac gataggcatt ctgccgggca gccgcatgtc
    14941 ggaaataaaa aaattgctgc cggaactgct tcaggccgcc acaatcatga aagacagcat
    15001 cccggagctg caatttgtcg tcccattggc cgacacgctt gaagagaaaa atctaaccgg
    15061 aatcattgcc ggatttccga tcgatgtcaa cgtcatttcc ggccagacct acgatgtcgt
    15121 ctcctgtgca gacctggcgc tggtcgcctc cggaacggcc acgctggaaa cagcgttact
    15181 gaaagtgccc atgattatcg tttacaaaat ttcgccgctg tcttatttta tcggaaaatt
    15241 attcgtccgc gtcaaaaata tcggcctcgt caacatcatt gccgagaaaa ccattgtgcc
    15301 ggaactgatt cagaacgatg ccagcggcgc ccggatcgcc agggaggccc tttccatttt
    15361 aggcgatgaa acaaaaaagc aggccatgat ccgggaactg gaggctatcc ggtcccggct
    15421 cggcgagccc ggggcggcaa ttaaaactgc taaactggca ttagatatgt tataggaaca
    15481 ccggaaacat catgaaaata tttaaacgtc ttttaagtct tggtcgtcca tacagcgtgc
    15541 gttttctgat ttccatgctc tgtatggtca tagccggtgc actgacatcg tcacaggcgg
    15601 tgctggtgaa acctgtgctg gacgacatct ttctggcaaa aaaccccgcg tcgctgaaat
    15661 ggctccctct cgccgtggtc atgatttttc tggtgaaagg aatctgtaat tacgttcaaa
    15721 cggttttaat gaattttatc ggcttgcgca ttgtcaccga tctgcgcaat cagctctacg
    15781 aacagataca gaaacagtcg ctgtcctttt ttacgcatca tccgacgggg ctgctcatgt
    15841 cgcgcattac caatgatgtc gcgtccattc aaaacgcggt gtccgatgtg gtcaccagta
    15901 tcgtgaagga ttcctttaca attgtcgccc tgatctgtgt cattttttat acggactgga
    15961 agctggcgtt cattgccatg attatttttc ctctcacgat ttatcctatt tccgcattcg
    16021 ggaagaaaat acgcaaggtc accacgtcga cgcagatcac catggccaat ctcagctcgc
    16081 ttttgcagga aaccattgcc ggaacgcgca tcgtcaaggc cttcggcatg gaaacgcacg
    16141 aaatcaaaag att
//
LOCUS       DAOSME010000052        15413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4568_length_15413_cov_19.869449, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000052 DAOSME010000000
VERSION     DAOSME010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15413
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_15413_cov_19.869449"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /gene="miaB"
                     /locus_tag="PLG98_08230"
     CDS             <1..1121
                     /gene="miaB"
                     /locus_tag="PLG98_08230"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOE33282.1"
                     /translation="QNPDLIIGVVGCLAQHLGNKFHKRMDYLDFVCGTHNIHQLPEII
                     DSVARDRQKITRIDFHPSLSSMSIFVPPRDGQISAFVTIMQGCNNFCAYCVVPYLRGR
                     EISRTPADILDEIGRLADAGVMEVTLLGQNVNSYGKTLHDGSNFASLIKKIGEIEGIK
                     RIRFTTSHPRDLSVEMIDCFRTEEKLCAHIHLPVQSGSNHVLALMNRGYTVEDYMQKV
                     DALRRTRPDISITSDMIVGFPGETEKDYQETIDMMEKIRFDSTFSFKYSEREGTAAAK
                     LKDSVPEGEKLRRLAALQDLQNRHTLEKNTQLLGTRQQVMVEGRSKNTANDLTGRTGS
                     WKIVNFAGPLELKGQTVVVGITEAYVHSLRGRIEKTEQ"
     gene            1134..1634
                     /locus_tag="PLG98_08235"
     CDS             1134..1634
                     /locus_tag="PLG98_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOE33283.1"
                     /translation="MLVEMKVLGLTIDPVTNTPIVILKDMDNNKAIPIWIGLFEASAI
                     ATEMKKIAFPRPLTHDLFVEFLRKFDITVNRIEIVDIRNNTFFANIYLMKEGLDYAID
                     ARPSDAIAIALRTNAPIFVDETVIEKSRNVDFGVKLGDLDKMKDDKLKEFLENLSMED
                     FGKYKM"
     assembly_gap    1681..1780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1845..1944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2273..3190
                     /gene="xerC"
                     /locus_tag="PLG98_08240"
     CDS             2273..3190
                     /gene="xerC"
                     /locus_tag="PLG98_08240"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOE33284.1"
                     /translation="MGNMIRDFQTYLDVEKNVSEHTKTAYIADIQEFARFLVEQQFVR
                     QPDEIVSTQPETLRHYLAVLYKKKMKKVTVNRKIASLRAFFKYLMRTGKIDVNPAEMV
                     QSLKTEKYIPTFLSVDEMLEVLNAQRDNSFSGLRDRAMLEVFYSCGLRLSELAGLDVA
                     DIDFNQKLVKVRGKGRKERIVPVGDPALKALTDYLEKARLIRTKSEPGVFKMPVFLNQ
                     RGERITTRSIARIVNASTVKSGIGRKISPHALRHSFATHLLNAGADLRSIQELLGHES
                     LSTTQKYTAVDINRLMEIYDKAHPRTKKP"
     gene            3336..3917
                     /gene="hslV"
                     /locus_tag="PLG98_08245"
     CDS             3336..3917
                     /gene="hslV"
                     /locus_tag="PLG98_08245"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOE33285.1"
                     /translation="MNIRGTTILAVKRDGKITVAGDGQVTLDTTILKHGARKVRRLYN
                     NEVIVGFAGATADAFTLFDRFDQKLEQYKGNLLRAAVELTKDWRTDRVLRHLEALMIA
                     VNRDNSLIISGNGDVIEADDDVMAIGSGGPFALTAARALLRHSSLNSTEIAREAMKLA
                     SEICIYTNEHITLEEIDIPSEDRERKGKDKGKK"
     gene            3921..5312
                     /gene="hslU"
                     /locus_tag="PLG98_08250"
     CDS             3921..5312
                     /gene="hslU"
                     /locus_tag="PLG98_08250"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOE33286.1"
                     /translation="MSNKGLKPREIVEKLNQHIIGQMDAKRAVAIALRNRWRRQNVLP
                     ELAEEILPKNIIMIGPTGVGKTEIARRLAKLDNSPFLKIEASKFTEVGYVGRDVESMV
                     RDLVELSVNMVKAEEQESVQAKAAEIAEERVLDLLLPPRLTEKKVDDILSDQPQEQTV
                     LTMEAEQKKNDATREKMRIWLKEGKFDDRMVELEVTEARSGPMIEIFSAAGMEDMGLN
                     LKDMLGNMMPQKKKRRTVKVPEALEILASEETQKLIDMEKVTRTALERVEQSGIIFLD
                     EIDKIVGGDSQHGPDVSREGVQRDLLPIVEGSNVNTRYGMVKTDHILFIAAGAFSGTK
                     PSDLIPELQGRFPIRVELDSLDKEDFIRILTEPNNALVKQYREMMATEGLRLVFKEDA
                     IAAIAEMATVVNENTENIGARRLYTMMEKLLDDISFDAPDLDKKDIVIDAQYVEEKLN
                     DIIEDQDLSRYIL"
     gene            5328..6218
                     /gene="argB"
                     /locus_tag="PLG98_08255"
     CDS             5328..6218
                     /gene="argB"
                     /locus_tag="PLG98_08255"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOE33287.1"
                     /translation="MENISNSIERADILLETLPYIRRFYNKTIVIKYGGHAMVDKDLK
                     DRFAQDIVMMKYIGINPVVVHGGGPQIGELLKKVGKESKFIQGMRVTDEETMNIVEMV
                     LVGMVNKEIVGLINRHGGNAVGLSGKDGNLITAEKYYLNEEKAKHTPPEIIDIGLVGK
                     VKSINTQLITSLAHGNFIPIIAPTGLGDNGETYNINADIVAGEIAAALKAEKLLLLTD
                     VPGVLSKKKELIHTMTNDEALNLIEDGTIEGGMFPKIKCCLKALNGGVQKAHIVDGRL
                     KHAVLLEVFTDKGIGTEIVL"
     gene            6242..7432
                     /locus_tag="PLG98_08260"
     CDS             6242..7432
                     /locus_tag="PLG98_08260"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOE33288.1"
                     /translation="MNEQEVIAQATGNIMNTYRRFPIVLTKGSGARVWDINGKEYLDL
                     VAGIAVCNLGHSHPQVIAAVKEQLDKLTHVSNLYYTQPQTELAELLIRHSFADKVFFC
                     NSGAEANEAAIKLARKYAHENMGPDKFELITMKDSFHGRTMATITATGQEKFQFGFTP
                     LLDGFTYVPFNDLSALDKAVSSRTCGVLLEPIQGEGGVNIPDDDYLARVREICDRHGI
                     LMILDEVQTGIGRTGKLFAYEYSAIKPDIMTLAKALGNGFPVGAMLATDKVAKAFVPG
                     NHASTFGGNPLAMAAGTAVLKTILQENLLEHCRKTGAYFLSQLKKLQQKHAVINEVRG
                     KGLMLAVKLNIDAAGIVGECLKQGLLVNSTGGQVLRFVPPLIITEQDIDQTVAVLDSV
                     LEGK"
     gene            7429..8337
                     /gene="argF"
                     /locus_tag="PLG98_08265"
     CDS             7429..8337
                     /gene="argF"
                     /locus_tag="PLG98_08265"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOE33289.1"
                     /translation="MKKDLLSLYDLDTKDFDIIWRKAGKLKKYLKAGKVHDSLKGKTL
                     GMIFDKSSTRTRISFEVGMYQLGGIALFLTSRDTQIGRGESVADSARMMSRYLNGIMI
                     RTFSQESVEELAQWATIPVINGLTDLLHPCQILSDLFTIIEKKGSYRNVKIAYIGDGN
                     NVANTWIEAAARLPIRLSLACPKGYEPDRTIMTKGRRESQEGIVLCHDPFEAAKNADV
                     LYTDVWVSMGQEAEDKKRKKIFKDYQINSNILKAAKKDAIVMHCLPAHRGEEISSDVI
                     DGPHSVVIDQAENRLHVQKAVLELLL"
     gene            8390..9604
                     /locus_tag="PLG98_08270"
     CDS             8390..9604
                     /locus_tag="PLG98_08270"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOE33290.1"
                     /translation="MAAKKRKVVLAYSGGLDTSVIVRWLIETYKCEVICFAADVGQKE
                     ELTGLKEKAIKTGASKIYIDDLREEFARDFVFPALRANAIYEGTYLLGTSLARPLIAK
                     RQIEIAKLEGADTVSHGATGKGNDQVRFELTYYALNPNIKIIAVWKDDNWTFRSREEM
                     IDYAGKYKIPIPLSKAKPYSADRNLLHISHEGGILEDPWAEPPEDIFVLTRSPENAPD
                     KPTYVEIDFKKGNPVAVNGKKMSPAKLMDYMNTVAGINGIGRVDMVENRFVGMKSRGV
                     YETPGGTVLWSAHRALESITMDREVMLMRDGLTPKYAQLAYNGFWYAPEMKALQTFID
                     ATQENVNGTVRMKLYKGNAIVAGRKSPNSLYSESFATFEKDQVYNQKDAIGFIKLNGL
                     RLIIENILKNKP"
     gene            9622..11010
                     /gene="argH"
                     /locus_tag="PLG98_08275"
     CDS             9622..11010
                     /gene="argH"
                     /locus_tag="PLG98_08275"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOE33291.1"
                     /translation="MSAGKKPWSGRFQKSTARSVEKFTASIHYDHRLYPWDIEGSIAH
                     ATMLAKQKIIAKKDAQKIIAALKAILKDFEQGKLRLDPADEDIHMAIEKELIRRIGEA
                     GGKLHAARSRNDQILLDERLYLRAEIQTIAGRLTSLQRALVQLAKREIKTIMPGYTHM
                     QKAQPVLLSHYLLAFADMFGRDVRRLQDCRERTNVLPLGAAALAGTGLPINRAETARL
                     LNFPGISTNSMDTVADRDFIAEFIFDAALIMMHLSRFCEDLVLWSSEEFGFIEISDAY
                     TTGSSIMPQKKNPDVAELIRGKTGRVYGDLFAVLTLLKGLPMTYNRDLQEDKEPLFDA
                     VDTVKSCLSIFCEMINNTRFHSAKMAASASKGFSTATEIAEYLVEKKVPFRRAHDITG
                     KIVAYCLRQKKDLQALTLKEYQAFYRGIQSDILDRIQPESAVRMRGHIGGTAPQTVLQ
                     RIKEFEKRFSKS"
     gene            10985..11437
                     /locus_tag="PLG98_08280"
     CDS             10985..11437
                     /locus_tag="PLG98_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33292.1"
                     /translation="MKSVSRRVKIFLAVGMLAILAACGVKGNPVGPPPGETHSPAVGN
                     LTVTSSADHVALTWALQDEHSRIRSIGIERIEYAAEDAECRNCADKFERIGRLLAGTT
                     GQNGLARKHFEFVDKNVTQGIRYGYRLSLCDEFDECFNSAVTEVYVSR"
     gene            11461..12342
                     /gene="dapA"
                     /locus_tag="PLG98_08285"
     CDS             11461..12342
                     /gene="dapA"
                     /locus_tag="PLG98_08285"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOE33293.1"
                     /translation="MFRGAMVAIVTPFKNDRIDEEALRDLIEFQIASGTDAIVPCGTT
                     GESATLTHEEHDRVIEITIDAVKKRVPVIAGTGSNNTAEALRLTKHAWEAGADGALIV
                     APYYNRPTQEGLYQHFKIIAESVPIPIIPYNIPGRTGVNMLPGMVAKLAQIKNIVGIK
                     EASGSIKQMSDVIDLCGKDFAVLSGDDIFTLPLMAIGGAGVISVISNVAPADMAGLID
                     AFHAGDMARAKSLHYKMAPLIEALFLEVNPIPVKAALALMGKINGEYRLPLCRMSEGN
                     FETLEKVMTDYGLIPPS"
     gene            12369..13181
                     /gene="dapB"
                     /locus_tag="PLG98_08290"
     CDS             12369..13181
                     /gene="dapB"
                     /locus_tag="PLG98_08290"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOE33294.1"
                     /translation="MVKAVVTGAAGRMGGKIISLISTMDNIRVVGAVEVSRHPIIGHD
                     IGQGLGLGRTGIMVCDTLSDCIGQADVVIDFTDHESSMKYLKIANESDKAIVIGSTGF
                     TPAEMESIKTLTKKTRCVLTPNMSVGVNVMLKVLEYCAGILKDDYDVEIIEAHHHLKK
                     DAPSGTAMQMARVIAEKLQRDPEECFVYSRHGLIGERTKKEIGIQTVRAGDIVGDHTV
                     IFGGVGERLEFVHRAHSRDNFAKGAIRAAQWIVSQKNGLYDMQDVLGLRTGK"
     gene            13198..13695
                     /gene="folK"
                     /locus_tag="PLG98_08295"
     CDS             13198..13695
                     /gene="folK"
                     /locus_tag="PLG98_08295"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOE33295.1"
                     /translation="MDGIICYIGIGSNLGDALDNCRKAVGLISRTEGISLERVSSFYK
                     TEPVGIQSQDDFVNAVIEIKTTLGAVDLLQKLQDVENTMGRTRAVKGGPRTIDLDLLF
                     YGQQVINAKGLVVPHPEAHKRRFVLEPLCDIASYFIHPSFGVSIRGLQNRLTDDKKVE
                     KLMEK"
     gene            13715..13966
                     /locus_tag="PLG98_08300"
     CDS             13715..13966
                     /locus_tag="PLG98_08300"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOE33296.1"
                     /translation="MIRLLISIAVIYILYKILKTFRQMKAEKSESRTPSSAGEDLVED
                     PVCHTYVPLSQAYKKDIDGKSYYFCSKDCSDKFPLEKNT"
     gene            13982..14629
                     /gene="fsa"
                     /locus_tag="PLG98_08305"
     CDS             13982..14629
                     /gene="fsa"
                     /locus_tag="PLG98_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOE33297.1"
                     /translation="MKFFIDTANIDEIKQGIALGMVDGVTTNPSLIAKEKKGFDVVVK
                     EILKNVQGPVSLEVVSHEAKEMFAEGKKLARLGSNVVIKVPLSTEGLKATKMFADAGI
                     DVNQTLIFSPLQALMAAKAGARYVSPFVGRLDDIAHDGMELVEQIITIFENYCYETEV
                     IVASIRHPRHVLDAALMGADIATIPFKVIAQLVQHPLTEKGIETFLNDWKKVPKK"
     gene            14667..15224
                     /gene="pgsA"
                     /locus_tag="PLG98_08310"
     CDS             14667..15224
                     /gene="pgsA"
                     /locus_tag="PLG98_08310"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOE33298.1"
                     /translation="MISRRDTFNLPNTITLLRIGVVPFLFILLSDPGKSWSLIIAGLF
                     VVASITDFFDGYIARKYQMITTMGKFLDPIADKIIVNSAMILLIPIGRIPAWIVAITI
                     IRDLMVDVIRSIASSEGIYIQASRLGKQKTVAQIIAVTALIIHYPFLGFNTHVVGTVV
                     LYVAFIFTIYSGIDYLVKLFQSIEQ"
BASE COUNT         4021 a         3488 c         4387 g         3317 t
ORIGIN      
        1 agcagaatcc ggatctgatc atcggcgtgg tgggttgcct ggcgcagcat ctcggaaaca
       61 aatttcataa gagaatggat tatctggatt ttgtatgcgg gacgcataac atccatcagc
      121 ttccggaaat catcgattcg gtcgcccggg acagacagaa aatcacccgg atcgattttc
      181 atccgtcgtt gagctcgatg agcatttttg ttccgccccg ggacggccag atcagcgcct
      241 ttgtgacgat tatgcaggga tgcaataatt tctgcgcgta ctgcgttgtc ccttatctgc
      301 gcggacgtga gatcagccgg acgccggccg acatcctcga tgaaatcggc cgacttgccg
      361 atgcgggagt catggaagtg acgctgctcg gacaaaatgt gaactcttac ggcaaaacgc
      421 tgcatgacgg atcgaatttt gcgtcgctga ttaaaaagat aggcgaaatc gaaggcatca
      481 aacggatacg gtttacgacg tcgcatccgc gcgatctgtc cgtcgaaatg atcgattgtt
      541 tcaggacgga agaaaaactc tgcgcacaca ttcatcttcc ggttcagtcc ggttccaatc
      601 acgttctggc gctgatgaac cggggatata cggttgagga ttatatgcag aaagtggatg
      661 ctttgcgcag gaccaggccc gacatcagca ttacttcgga tatgatcgtc ggatttccgg
      721 gagaaaccga aaaagattac caagaaacaa ttgacatgat ggaaaaaatc aggtttgatt
      781 caactttctc tttcaagtat tctgaaaggg aggggacagc ggccgcaaaa ttaaaagact
      841 cggttccgga aggcgaaaag ttaagacgcc tggcggcgct gcaggatttg cagaatcggc
      901 acacgctgga aaaaaatacg caactgctgg gaacgcggca gcaggtgatg gtggaaggcc
      961 gaagcaaaaa taccgccaat gatctgacgg gacgaacggg atcatggaaa attgtcaatt
     1021 ttgcgggccc tttagagttg aaaggccaaa cggttgtcgt cggcataacg gaagcgtatg
     1081 tgcattcttt aagaggccgg atagaaaaaa cagaacaata ggagagattg tccgtgttag
     1141 ttgaaatgaa ggttctgggt ttgaccatcg atcccgtaac caatacgccg attgtgatat
     1201 tgaaggatat ggataacaac aaggccatcc cgatctggat cggtttgttt gaggccagcg
     1261 cgattgccac ggaaatgaaa aagattgcgt ttcctcgacc gctgacccac gatctgtttg
     1321 tcgagttttt gcgcaagttc gatattaccg taaaccggat tgaaattgtc gatatccgca
     1381 acaacacttt tttcgctaat atttatttga tgaaagaggg gctggattat gccattgacg
     1441 cacgtcccag cgatgcgatc gccatcgccc tgagaaccaa cgccccgata ttcgtggatg
     1501 aaacggtcat cgaaaaatcg cgcaatgtcg atttcggcgt caagctgggc gatctggata
     1561 aaatgaaaga tgataaactg aaagaatttc tggaaaatct ttccatggag gatttcggca
     1621 aatataagat gtaatgaggc tcgctaaaga cgagcgcagc gaagtctgaa gcgcaagccg
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aagatgtaat gaggctcgct
     1801 aaagacgagc gcagcgaagt ctgaagcgca agccgaatta tcccnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnngaatgt gggtgaggct cgctaaagac gagcgcagcg
     1981 aagtctgaag cgcaagccga attatccccg gagcgaagcg aatggggatt taagacttga
     2041 aacgtattga atgtgggtga ggctcgctaa agacgagcgc agcgaagtct gaagcgcaag
     2101 ccgtagtgag attcgctgag aatgagtgaa acgaaattca aagcgtaaat cgaactatcc
     2161 aattccgatt tgtcgggatt gggaattatc cccggagcga agcgaatggg gattgaaggt
     2221 gttctgccgg gttatcagga tgggtgtctg acggctagcc ggaataaaag ttatgggaaa
     2281 catgattcgg gattttcaaa cgtatctgga cgtggaaaaa aatgtttccg aacataccaa
     2341 aacggcttat atcgcggaca ttcaggaatt cgcgcggttt ctcgtcgagc aacagtttgt
     2401 gagacaaccg gatgaaatcg tctcgacgca accggaaacg ctcaggcatt acctggcggt
     2461 gttgtataaa aaaaagatga agaaggtcac ggtgaaccgg aaaatagcgt cgctgcgggc
     2521 tttcttcaaa tatctgatgc gcacgggaaa gatcgatgtg aatcccgctg aaatggtgca
     2581 gtcgctgaaa accgaaaagt atataccaac gtttttgtcc gtagatgaaa tgctggaggt
     2641 tttgaacgcg cagcgtgata attcgttcag cggactgcgc gaccgcgcca tgctggaggt
     2701 cttttattcc tgcggactgc gcctgagtga actggccggg ctcgatgtgg cggacatcga
     2761 tttcaatcag aagctggtca aggtcagggg caagggcaga aaagaaagaa ttgttccggt
     2821 gggagacccg gctctgaagg ccctgacaga ttatctggag aaggcccgtc tgatcagaac
     2881 aaaaagcgaa ccgggtgtgt tcaaaatgcc cgtgtttctg aatcaaagag gtgaaaggat
     2941 cacaacaaga agtatcgcga gaatcgtcaa tgcgtcgacg gtgaaaagcg ggatcggccg
     3001 gaaaatcagc ccgcatgcgc tgcggcacag ttttgcgacc catctgctga atgccggcgc
     3061 cgatttgcgg tcgattcagg aattgctcgg gcatgagagc ctttccacaa cgcagaaata
     3121 tacggcggtg gatatcaacc ggttgatgga gatttacgat aaggcgcatc cccggacgaa
     3181 aaagccttag tcgaatttat cccgttcgcg cgaagcggga atcgggatct gaaaacacaa
     3241 gggcacttgg cttttttcaa ggcgaagccg cagagaaaag ccttcgtccg atttttgttg
     3301 ttgtttccgg aataattttt atttaaggag aagaaatgaa tatacgcggc acaaccattc
     3361 tggcggtgaa acgggacggt aaaataacgg ttgccggaga cggacaggtg acgctggaca
     3421 cgacgatcct gaagcacggc gcgcgcaagg tccgccgcct gtataataat gaagtgatcg
     3481 tcggctttgc cggagcaacc gccgatgcgt ttacgctgtt cgaccgtttc gatcaaaaac
     3541 tggagcagta caaaggcaat ttgctcagag cggccgtgga gctgaccaag gactggcgca
     3601 ccgaccgggt tttaagacat ctggaagccc tgatgatcgc cgtcaacagg gacaattctc
     3661 tgattatttc cggcaacggc gacgtcattg aagccgatga cgatgtcatg gcgatcggtt
     3721 cgggcggccc gtttgcgctg acggcggcac gggcgctgtt gcgccattcc agtttgaatt
     3781 cgacggaaat cgcccgagag gcgatgaagc tggcttcgga aatctgtatt tacaccaatg
     3841 agcacattac cctggaagaa atcgatatcc cttccgagga cagggaaaga aaagggaagg
     3901 ataaaggaaa gaagtaaaac atgtctaata aaggattaaa accacgggaa atcgtggaga
     3961 agttgaatca gcatattatc ggtcagatgg acgccaaaag ggcagtggcc atcgctttgc
     4021 gcaaccgctg gcgcaggcag aatgtattgc cggaactggc ggaggaaatt ttaccgaaaa
     4081 acattatcat gattggcccc accggcgtgg gaaaaacgga aatcgcccgc cggctggcca
     4141 agctggataa ttccccgttt ttaaagatcg aggcgtccaa atttacggaa gtggggtatg
     4201 tcggccggga tgtggaatcc atggtgcggg atctggtgga actgtccgtg aacatggtca
     4261 aggcggagga acaggaaagc gttcaggcga aagcggcgga gatcgccgag gaaagggttc
     4321 tggatttgct tttgccgccg cggctgacgg aaaaaaaagt tgatgatatc ctcagcgatc
     4381 agccgcagga acagaccgtt ctgaccatgg aggcggaaca aaagaaaaat gatgcgacac
     4441 gcgaaaaaat gcgcatctgg ctcaaggaag ggaaattcga cgaccgtatg gtagaactgg
     4501 aagtgaccga agcgagaagc ggaccgatga tcgaaatctt ttccgccgcc ggcatggagg
     4561 atatgggact gaatctgaaa gacatgctgg ggaacatgat gccgcagaag aaaaaacgaa
     4621 ggacggtcaa ggttccggag gcgctggaaa ttctggcctc ggaagaaacc cagaagttga
     4681 tcgatatgga gaaggtcacc cggacggcgc tggaacgcgt cgagcagtcc ggcattattt
     4741 tcctggatga aatcgataag attgtcggcg gggattccca gcacggtccg gacgtatcac
     4801 gggaaggtgt gcagcgcgat ctgctgccga ttgtggaagg ctccaatgtc aacacgcgtt
     4861 acggcatggt caaaacggat catattttat tcatcgccgc aggggcgttt tccggcacga
     4921 agccgtcgga cctgattccg gaacttcagg gacgtttccc gattcgcgtg gaactggatt
     4981 cgctggataa ggaggatttc atccgcatcc tgacggaacc gaataatgcg ctggtgaaac
     5041 agtacaggga aatgatggcg acggaagggc tgaggctggt tttcaaggag gatgccattg
     5101 ccgcaatcgc ggaaatggcg acagtggtta atgaaaatac ggaaaatatc ggtgcgcgaa
     5161 ggctctacac catgatggaa aaattgctgg atgacatttc cttcgatgcc ccggatctgg
     5221 ataaaaagga cattgtgatc gacgcgcagt atgtggaaga gaaactcaac gacattatcg
     5281 aagatcagga tttgagcagg tatattttat aaaaatggag attggaaatg gaaaatattt
     5341 ctaattcgat tgaaagggcg gatattcttc tggaaacgct gccgtatatc aggcggtttt
     5401 acaacaagac gattgtgatc aaatacggcg gtcatgccat ggtggacaag gacttgaaag
     5461 accggttcgc ccaggatatc gtgatgatga aatatatcgg catcaatccg gtggtcgtgc
     5521 acggcggcgg cccgcagatc ggcgagcttc tgaaaaaagt cggcaaggag tcgaaattta
     5581 tccagggaat gcgggtgacc gatgaggaga ccatgaatat cgtggaaatg gttctggtcg
     5641 gcatggtcaa caaggaaatt gtcggcctga tcaaccggca cggcgggaac gccgtgggat
     5701 taagcggcaa ggacggcaac ctgattacgg cggagaagta ttatctgaat gaagaaaagg
     5761 caaaacatac gccgccggag attatcgata tcggcctggt gggcaaggtc aaaagcatca
     5821 atacccagtt gatcacgtcc ctggcgcacg gcaattttat tccgattatc gcgcccacgg
     5881 gactcggcga taacggcgag acgtacaaca tcaatgcgga tatcgtcgcc ggagaaatcg
     5941 cggcagcgct gaaagccgaa aagctcctgc tgctgaccga tgtgcccggt gttttgagca
     6001 agaaaaagga attgatccat acgatgacca atgacgaggc gttgaatctg atcgaggacg
     6061 gaacgatcga aggcggcatg tttcccaaga tcaaatgctg tctcaaggcg ctcaacggcg
     6121 gcgtccaaaa agcccatatc gtcgacgggc ggcttaaaca tgccgtactt ctggaggtgt
     6181 tcaccgacaa gggcatcgga acagaaatcg ttctttaaaa tattacaaac aggacaggac
     6241 catgaatgaa caggaagtga tagcgcaggc tacaggaaat atcatgaata cgtacaggcg
     6301 gtttccgatc gtattgacga aagggtcggg cgcccgggtc tgggatatca acggcaagga
     6361 atatctcgat ctggtggccg gcattgccgt ctgcaatctc gggcattcgc atccgcaggt
     6421 gattgctgcc gtcaaggagc agctggataa actgacgcat gtttccaatc tgtattacac
     6481 ccagccgcaa accgagctgg cggagctgct gatcaggcat tcttttgccg acaaggtttt
     6541 tttctgcaac agcggcgcgg aggccaacga agcggccatc aaactggcgc gcaagtacgc
     6601 ccatgaaaat atgggtccgg ataaattcga actgattacg atgaaggatt cgtttcacgg
     6661 ccgcaccatg gccacgatta cggccaccgg tcaggaaaaa tttcagttcg gtttcacgcc
     6721 gcttctggac gggtttacct atgttccttt caacgatctg tcggcgctgg ataaggccgt
     6781 ttcgagccgg acctgcggcg tcctgctgga accgattcag ggggagggcg gtgtcaatat
     6841 tcccgatgat gattatctgg cgcgggtgcg cgaaatctgc gaccggcacg ggattctgat
     6901 gattcttgat gaagtgcaga ccggcatcgg cagaacgggg aagctgtttg cctacgaata
     6961 ttccgccatt aaaccggata tcatgacgct ggccaaagcg ctgggaaacg gtttcccggt
     7021 gggcgcgatg ctggccacgg acaaggttgc caaggcgttt gtccccggca atcacgcgtc
     7081 aacattcggc ggcaatcctc tggccatggc ggcgggaacg gccgtattga aaaccattct
     7141 gcaggagaat ttgctggaac attgccggaa aaccggcgcg tattttctat cgcaattgaa
     7201 aaaattgcag cagaagcatg ccgtgatcaa cgaagtccgg ggcaaggggc ttatgctggc
     7261 cgtaaaactg aatatcgacg cggcgggcat tgtcggcgaa tgtctgaaac agggcctgct
     7321 cgtcaattcc acaggcggtc aggtgctgcg gtttgttccg cccctgatca ttaccgaaca
     7381 ggatattgat cagacggtgg cggtgcttga cagcgtattg gaggggaaat gaaaaaagat
     7441 ttattgagcc tttatgatct cgacacgaaa gattttgata tcatctggag gaaagccggc
     7501 aaactgaaaa aatatctgaa agccggcaaa gtccacgatt cgctgaaggg gaaaacgctg
     7561 ggaatgattt tcgacaagtc ctccacgcgg acgagaattt ctttcgaggt cgggatgtat
     7621 cagctcggcg gcatcgctct gtttctgacg tcccgcgaca cccagatcgg ccggggcgaa
     7681 tccgttgccg attcggcgcg gatgatgtcg cgctatctca acggcattat gatccggaca
     7741 ttttcgcagg aaagcgtgga ggaactggcg cagtgggcga cgattccggt aatcaacggt
     7801 ctgacggatc ttctgcatcc ctgtcagatt ctaagcgatc tgtttaccat tatcgagaag
     7861 aaggggtcct atcgtaacgt caagatcgcc tacatcggcg acgggaataa cgtggccaat
     7921 acctggatcg aagcggccgc ccggctgccg atccgtctgt cgctggcctg tccgaaaggc
     7981 tatgagccgg accgaacgat catgacgaag ggccgccgcg agtcgcagga aggcattgtg
     8041 ctgtgccacg atccgtttga agcggcgaaa aacgcggatg tgctctatac cgatgtctgg
     8101 gtcagcatgg ggcaggaagc ggaggataaa aaaaggaaaa aaatatttaa agattatcag
     8161 atcaacagca acattttgaa ggcggccaaa aaagatgcca tcgtgatgca ttgtctgccg
     8221 gcgcaccggg gcgaggagat ttcctccgat gtcatcgacg gcccccattc ggtggtcatc
     8281 gatcaggcgg aaaaccgtct gcatgtgcaa aaggccgttc tggaattgtt gctgtaacga
     8341 tttgatcaac atattcatcc cgcaaatcgg ggtcaggagg acatccacat tggcagcaaa
     8401 gaaaagaaaa gttgttttgg cgtattccgg aggactggac acatcggtta ttgtccgctg
     8461 gttgattgaa acctataaat gcgaggtcat ctgctttgcc gcagacgtcg gacagaagga
     8521 ggaattgacc ggcctgaaag aaaaggcgat caaaaccgga gcgagcaaaa tttacattga
     8581 cgatctgcgg gaggagttcg cgcgcgattt tgttttcccc gcgcttcggg ccaacgcgat
     8641 ttatgaaggc acctatcttc tgggcacatc gctggcgcgg ccgctgatcg ccaaacggca
     8701 gatcgaaatt gcgaagctgg agggcgccga tacggtttcc cacggcgcaa ccgggaaggg
     8761 caatgatcag gtccgcttcg aactgaccta ttatgcgctc aatccgaata tcaaaatcat
     8821 cgcggtctgg aaggacgaca actggacctt ccggtcccgg gaagagatga tcgattatgc
     8881 cgggaaatac aagattccca ttccgttgag caaggccaaa ccctacagtg cggacagaaa
     8941 tcttctgcat atttcgcatg aaggcggaat tctggaagat ccgtgggccg aaccgccgga
     9001 agatattttt gtattgaccc gctcgcccga aaatgcgccg gacaaaccga cgtacgttga
     9061 aatcgatttc aagaagggaa atccggtggc cgtcaacggc aaaaagatgt cgccggcaaa
     9121 actgatggat tatatgaata cggttgccgg gataaacggc atcggccgtg tcgatatggt
     9181 ggagaaccgt tttgtcggca tgaaatcgcg gggcgtttac gagacccccg gcggaaccgt
     9241 gctctggtcg gcgcaccgcg cgctggagtc catcacgatg gaccgtgaag taatgctgat
     9301 gcgcgacggc ctgacgccga aatatgcgca gttggcctac aacgggttct ggtatgctcc
     9361 ggaaatgaag gcgctgcaaa ccttcattga tgccacgcag gaaaacgtca acggcacggt
     9421 gcggatgaaa ttatacaagg gcaacgcgat cgtggcgggc cgcaaatcgc cgaattccct
     9481 ttacagcgaa tcgttcgcca cctttgaaaa ggatcaggtg tacaaccaga aggatgccat
     9541 cggtttcatc aagctcaacg gattgcggct gattatcgaa aatattttga aaaataaacc
     9601 ttaatggatc ggggagaaaa aatgtctgcc gggaaaaagc catggagcgg aaggtttcag
     9661 aaatccacag ccaggagcgt cgaaaaattt acagcgtcca ttcattacga tcataggctc
     9721 tatccctggg acattgaggg atcgattgcc catgcgacga tgctggcgaa acagaagatt
     9781 atcgccaaaa aggatgcgca gaaaatcatc gccgcgttga aggcgattct gaaagacttc
     9841 gagcagggaa aactccggct ggatcccgcc gatgaagaca tccacatggc catcgaaaag
     9901 gaactgatcc ggcgcatcgg agaggcggga ggcaaactgc atgcggccag aagccgcaac
     9961 gatcagattc tgctggatga aaggctttat cttcgggcgg aaattcagac catcgcagga
    10021 cgtctgacat cgttacagcg cgccctggtc caactggcca aacgggaaat caaaacgatc
    10081 atgcccggct atacgcatat gcagaaggcg cagccggtgc tgctgtcgca ttatcttctg
    10141 gcgtttgcgg atatgttcgg acgcgatgtg cggcggcttc aggattgccg cgaaaggacg
    10201 aatgttctgc cgctgggcgc ggcggcgctg gccggaacgg ggctgccgat taaccgcgcg
    10261 gaaaccgcca ggctgctgaa ctttcccggg atcagcacaa acagcatgga tacggtggcg
    10321 gaccgggatt ttatcgcgga gtttattttc gatgccgccc tgatcatgat gcatttaagc
    10381 cggttttgcg aagatctggt tctgtggtcg tccgaggagt tcggttttat tgaaatatcg
    10441 gatgcctata cgaccggcag cagcatcatg ccgcagaaaa aaaatccgga tgtcgcggag
    10501 ctgatccggg gaaaaacagg gcgggtatac ggcgatctgt tcgcggttct gacgctttta
    10561 aaagggctgc ccatgacata caaccgggat ttgcaggaag acaaggagcc gcttttcgat
    10621 gcggtggata cggtgaaaag ctgcctttcg attttttgcg agatgataaa caacacccgg
    10681 tttcattcgg cgaagatggc cgcatcggcg agcaagggat tttccaccgc caccgaaatt
    10741 gcggaatacc tggttgaaaa aaaggtgcct ttccggcggg cgcacgacat caccggtaaa
    10801 attgtggcct actgtctgag gcagaaaaaa gatttgcagg cgctgacgct gaaggaatat
    10861 caagcctttt accgcgggat tcaatcggat atcctcgaca ggattcaacc ggaaagcgcg
    10921 gtccggatgc ggggccatat cgggggaacc gctccgcaaa cagtattaca gaggattaaa
    10981 gaatttgaaa agcgtttctc gaagagttaa aatatttctg gcggtaggga tgctggcgat
    11041 actggcggcc tgcggcgtca aaggaaatcc tgtcggtccg cccccgggcg agacgcattc
    11101 cccggcggtc ggcaatttaa ccgttacttc ctcggcggat catgtggcgc tgacgtgggc
    11161 gttgcaggac gagcattcac gaatccgttc catcggcata gaacgcatcg aatacgcagc
    11221 cgaagatgcc gaatgccgaa attgcgcgga taaatttgaa cgcatcggac gtttgttggc
    11281 cggaacaacc ggacagaacg gactggcccg aaagcatttt gagtttgttg ataaaaatgt
    11341 cacgcaaggc atacgctacg gctatcgttt gtcactgtgt gacgaatttg acgaatgttt
    11401 taacagtgcg gtaacggaag tatatgtttc gcgatgaatt gacagaagga ggcttctttt
    11461 atgtttagag gcgcaatggt tgcaattgtc accccattta aaaacgaccg aatcgacgag
    11521 gaggctctgc gcgacctcat cgaatttcag attgccagcg gcaccgacgc gatcgtcccc
    11581 tgcgggacaa ccggcgaatc ggccacgctg acccatgaag agcatgatcg cgtcatcgaa
    11641 attacgatcg acgccgtcaa aaagcgggtg ccggtcatcg ccggaaccgg ctccaacaac
    11701 acggccgagg ccctgcgatt gaccaagcat gcctgggagg cgggagccga cggcgcgctg
    11761 atcgtcgccc cctattataa tcgtccgacg caggaggggc tttatcagca tttcaaaatc
    11821 atcgccgaga gtgtgcccat tcccatcatt ccctacaaca taccgggacg caccggtgta
    11881 aatatgctgc ccgggatggt ggcgaaactg gcgcagatca agaatatcgt cggaatcaag
    11941 gaggcctcgg gctccatcaa acagatgagc gacgtgattg atctgtgcgg aaaagatttt
    12001 gccgttttat ccggggatga tatattcacg ctgccgttga tggccatcgg cggggccggt
    12061 gtgatttccg tgatttcgaa tgtggcgccg gcggacatgg ccggcctgat cgatgccttc
    12121 catgcgggag atatggcccg ggcaaaatcg ctgcattaca aaatggcgcc tttgatcgaa
    12181 gccctgtttc tcgaagtcaa cccgattccg gtcaaggcgg cgcttgccct gatgggtaaa
    12241 atcaatggcg aataccgatt gccgctgtgt agaatgtccg aaggcaattt tgaaacgtta
    12301 gagaaggtca tgacggacta cggattgatt ccgccgtcgt gatgtcataa agaaaaggag
    12361 atcaacctat ggtaaaagcc gtggtaaccg gggctgccgg aagaatgggc ggcaaaatca
    12421 tcagtctgat ttcgaccatg gacaacatca gggtggtcgg cgccgtagag gtgtccagac
    12481 atcccattat cggccacgat atcggccagg ggctgggact ggggagaacc ggtataatgg
    12541 tttgcgatac cctgtcggac tgtatcggcc aggcggatgt ggtcattgat tttacggatc
    12601 acgaatcgtc catgaagtat ctcaagattg ccaatgaaag cgacaaggct atcgtcatcg
    12661 gttccacggg ctttacacct gccgagatgg aatcgatcaa aacgctgacc aaaaagaccc
    12721 gttgcgtgct gacgccgaat atgagtgtgg gcgtcaacgt aatgctgaag gttctcgaat
    12781 attgcgcggg cattctgaag gacgattacg atgtcgagat tatcgaggcg catcatcacc
    12841 tgaaaaagga cgcccccagc gggacggcca tgcagatggc cagggtgatc gcggaaaaac
    12901 ttcagcggga cccggaggaa tgttttgttt attcccggca cggcctgatc ggcgagagga
    12961 cgaaaaagga aatcggcatt cagaccgttc gcgccggcga tatcgtggga gatcatacgg
    13021 tgattttcgg cggtgtcggg gaaaggctgg aatttgtcca ccgggcgcac agccgggaca
    13081 actttgccaa aggggcgatc cgggcggcgc agtggattgt cagccagaaa aacggtctgt
    13141 atgatatgca ggatgttctg ggattaagga ccgggaaata ataaggacga gcttttattg
    13201 gacggtatca tctgctatat cgggatcggg tccaatctcg gcgatgcttt ggacaattgc
    13261 cggaaagctg tcgggctgat ttcgcgaacg gaggggatca gcctggaaag ggtgtcatcc
    13321 ttttataaga cggaaccggt tgggattcaa tcccaggatg attttgtcaa tgcggttatt
    13381 gaaattaaga caacgctcgg ggctgtcgat ctgttgcaga agctgcaaga cgtggaaaac
    13441 actatgggcc gaacccgggc ggtgaaaggc ggaccgcgga cgattgatct cgatcttctt
    13501 ttttacgggc agcaggtgat caacgcgaag gggctggtag tcccgcatcc cgaagcccac
    13561 aaacgacggt ttgttctgga gccgctttgc gacatcgcct cgtatttcat tcacccgtca
    13621 ttcggcgtgt ccatccgggg attgcaaaat cggctgacgg atgataaaaa agtggaaaaa
    13681 ttgatggaaa aataacgatg aaggtggttt tatattgatc agattactga tatccatagc
    13741 ggtcatttac atactgtata aaattttaaa aacattccgg cagatgaaag cggaaaaaag
    13801 cgagagcagg accccgtcgt cggccggaga agatctggtg gaagatccgg tgtgccatac
    13861 atatgttccg ttgagtcagg cttacaaaaa ggacatcgac ggcaagtcct attatttttg
    13921 cagcaaagat tgcagcgata aatttccatt ggaaaaaaat acataatgca aggagctttt
    13981 tatgaagttt tttatcgaca ccgccaatat cgatgaaatc aaacagggga ttgccctagg
    14041 gatggttgac ggcgtgacga ccaatccgtc cctgatcgcc aaggagaaga aagggtttga
    14101 tgtcgttgtg aaggaaattc ttaaaaatgt ccagggtccg gtcagcctgg aagtggtcag
    14161 tcatgaggcg aaggagatgt ttgccgaagg aaaaaaactg gcgcgcctcg gcagcaacgt
    14221 tgtgatcaag gttcctctgt cgacggaagg cttgaaggcg acaaaaatgt ttgccgatgc
    14281 cggtatcgac gtcaatcaga cgctgatctt ttctcccctg caggcgctga tggcggccaa
    14341 ggccggggcc cggtatgtca gtccctttgt cggcaggctg gatgatatcg cgcatgacgg
    14401 catggaactg gtggaacaga tcatcaccat ctttgaaaat tattgctatg agacggaagt
    14461 gattgtggcc agtatccgcc atccccggca tgttctggat gccgcattga tgggcgcgga
    14521 tatcgccacg attcccttta aggttatcgc ccaactggtt cagcatccgc tcacggagaa
    14581 aggcatcgag acgtttttaa acgattggaa gaaggttcca aagaaataag caaacggttg
    14641 atgcgattta tggacaaggc attccaatga tatcgagacg ggatacattc aatctgccca
    14701 acacaatcac cctgctgagg atcggcgtcg tgccttttct ttttattctg ctttcggatc
    14761 cggggaaatc ctggagtctg atcatcgcgg gattgtttgt ggtggcctcc attacggatt
    14821 ttttcgacgg ttatatcgcc cgaaaatacc agatgatcac aaccatggga aaatttctgg
    14881 accccattgc cgacaaaatc atcgtcaatt cggcgatgat cctgctgatt cccatcggac
    14941 gcataccggc atggatcgtt gccatcacga tcatccgcga tctgatggtg gacgtgatca
    15001 ggagtattgc gtcttcggaa ggaatttata ttcaggccag ccggctggga aagcagaaaa
    15061 ccgtcgcgca gatcatcgcc gtgacggcgt tgatcattca ttatcctttt ttgggtttca
    15121 acacccacgt tgtcgggacc gtcgtccttt atgttgcgtt tattttcacg atttattccg
    15181 ggattgatta tctcgtgaaa ttgtttcaaa gcattgaaca ataaggattg cccaccggat
    15241 ttttcaaccg ccgtcttcac catcgcctcg gaagaagcgt cgtgcatttt tttatgaata
    15301 aaatattaat ttttattgac aagaagcagc gatttgttat agtcaacgcc gttttaagtg
    15361 agcgggcgta actcagcggt agagtgctac cttgccaagg tagacgtcga cgg
//
LOCUS       DAOSME010000053        14774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4780_length_14774_cov_23.957470, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000053 DAOSME010000000
VERSION     DAOSME010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14774
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_14774_cov_23.957470"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLG98_08315"
     CDS             complement(<1..727)
                     /locus_tag="PLG98_08315"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOE33299.1"
                     /translation="MLPDIKTVELFRIGVLHISPFGVLAATGILTGRALALHRAKQAG
                     IPEEDMDDALLYTLLSAFFFAHIAEAVFYHPDQIMRQGPVYLLKFWKGLSSFGGFFGG
                     LFGFMFFVYRRKIRSPLLFAECILQGLVLGWIFGRLGCTIIFDHPGQLSDFFLAFQQP
                     GGARHNLGFYEFLLTLLVLFPVTLVLHRLQARPGSYTAAVLILYTPVRFFLDFLRLDA
                     AQNGDIRYGALTPGQYGSMILFVL"
     gene            1026..2849
                     /locus_tag="PLG98_08320"
     CDS             1026..2849
                     /locus_tag="PLG98_08320"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOE33300.1"
                     /translation="MKVLVINSGSSSLKYEVFDLNAEQSLMSGAVSRIGTKEADHACA
                     CGDKKQLTKIEAPDHRAALDFVLQAITGMEGTPIKNLEEIDAVAHRIGHGGSLYRGPV
                     VIDQSVIDEIRRLIPLMPLHHPAMLAGIDACQKALPDIPHVAVFDTSFHSAIPDEAAI
                     YGLPHEFFTAGVRKFGFHGNSHEYVASKAAEYLETPMRRLNLISCHLGNGASVCAIQR
                     GHSIDTSMGFSPLEGLIMGTRIGDLDPGIIPYLMRQHHLDIDALDKMLNNKSGLLGIS
                     GVSSDMREIIAAADADNARALLAIKAFCYRIKKYIGAYHGVLGGADALIFTGGIGENN
                     RSIRSRCVQGLEKLGFAIDKVRNDRCEVHAEKPVCDISARYANTQILVIATDEELMIA
                     RQCARALDYTSSIKHHLLATDRRPIRVSVSVRHAHLSKSDVEALFGPGYVLTSKSPLY
                     IDSEFAANETVDLVGPRGRVNNVRIIGPERLKTQVEISRTEEFQLGIDAPVRESGDLD
                     GTPGIILEGPAGRIEIPEGVICAMRHIHMTPEDAELYGVKDKDIVMVRIEGDRELIFG
                     DVLVRVKPNYKLEMHIDTDEANAAELPPVSEGYLVRIESRN"
     gene            3239..4558
                     /locus_tag="PLG98_08325"
     CDS             3239..4558
                     /locus_tag="PLG98_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33301.1"
                     /translation="MTKKKLKIMLINPPNCGRSIPEEEYGITSLKQIFKGEPFNLEVL
                     AGPLIDHDVMIMDLKCEPDGALWKAFDDFQPDVVGLTSLTCEANTVIGIARGVKEKSN
                     AIVVIGGNHATYNPEYFNRADFDYIAVGLAKKSFRDLIDHLAFGREPTGLAGIARCAP
                     GAPLAYTKRSFDEKDLMDDIPPRYDLVAQYRDRYFLEHLGLTMGFVITAYGCTHRCSF
                     CTIPEMTGGKYLNHSAGAVLRDIQLLGDIPFIRMVDANTFGNLSLSLDLYAKIMQSGI
                     RKKFFADVRADTIVKNPDLMKKWKEAGLYAVVIGFEDFQDARLDGYNKKYQGRVIHEA
                     IEILHDLGIIMVGDFIASPDYDESDFEKLEAFIVAHQIQIPVISVLTPIPGTPLYEKM
                     KKDIVTDDLDYYTFTNAVTKTKMPEARFYKTFADMVKRLHRQPHKTT"
     gene            4571..5713
                     /locus_tag="PLG98_08330"
     CDS             4571..5713
                     /locus_tag="PLG98_08330"
                     /inference="COORDINATES: protein motif:HMM:NF005293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOE33302.1"
                     /translation="MDVYINDIAAFLPNDPVTNDEMETVLGAVHNIPSRIKNRILKNN
                     GIEKRYYAIDRKTGELNYTNARLTAEAVRRLKPYEGFSVNDIQMLCCGTSTPDVLLPG
                     HGLMVQGELGIPSCEVITTAGICLCGITSFKTAYANVALGLSDNAVSTGSELASSLIK
                     SNFFEHLHGDRDFKNHPVLPFESDFLRWMLSDAAGAVFMSGKKNRNGLSLRVDWIENI
                     AYAGQMKTCMYAGAHKKEDGTVIGWRQLHSLEEALSSNYFALKQDTELLNREIVRVMI
                     DLGLSHTVERRKIKPDDIDWYLPHYSSDFFREKIFNAMEGIGFRIPFEKWFTNLAYKG
                     NTGSASIYVIMEELFQSGQLKSGQKLLCFIPESGRFSVAYVLLTVV"
     gene            complement(5722..6012)
                     /locus_tag="PLG98_08335"
     CDS             complement(5722..6012)
                     /locus_tag="PLG98_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOE33303.1"
                     /translation="MEQEFKTVSVMIDLYCRGHHDSRDHRCVDCEALLTYVGQRLEKC
                     PLKTNKPKCSKCPVHCYKPDMREKIKAVMRYAGPRMIYRHPILAGKHFLNKN"
     gene            complement(6019..6756)
                     /locus_tag="PLG98_08340"
     CDS             complement(6019..6756)
                     /locus_tag="PLG98_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOE33304.1"
                     /translation="MKILRKIIEIEEELCNGCGNCVTGCAEGALQIIDGKAKVVSETF
                     CDGLGACIGECPTGALKIVEREASPFDEEAVKKHLAKKEKEPETMPCGCPSTHIQIFD
                     KPSACAEANKPKHFDNVEQESVLTHWPIQIKLIPASAPFLKGGDLLILADCTAVAFPT
                     LHRDLMKGKVVMMGCPKFDDVPEYVQKFADIFKTAGIRSVTVVVMEVPCCSGMPMIVK
                     KGMEAAGKEIPMKEIVLSLRGKILKSS"
     gene            complement(6760..8283)
                     /locus_tag="PLG98_08345"
     CDS             complement(6760..8283)
                     /locus_tag="PLG98_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOE33305.1"
                     /translation="MSSERNEQNIVIVSRGNEVIETITLGGHMIIGRAAGADIQLEDT
                     ALSRIHAMFQKDAGRWFLVDLNSLNGTLLNGRKIAPMQPTAVNSGDIAELGEYILRFQ
                     IESQPVAPDQTRPRFVTPDIEPPPSASPLLENLVERHHEIPLWTQGDIQLKIADIIDE
                     TDDVKTFRLVGLSPVLFSFKPGQFVTIKVNINGTDVRRSYTISSSPSRPHCLEMTIKR
                     TPQGLVSNWMHDHVKLGDHLAVHGPSGLFSCFNYPSQKILFIGAGSGITPVMSMLRWI
                     VDTAADVDARLLVSAKSPREIIFKNELDWMSSRHSGIRVGITCTSRVTGADAWTGLTG
                     RCDEKMLKLFTPDILERHIFMCGPSPFMEAVKENLKLLGFNLAHLHTESFGEGRVAKG
                     EKVDARDVPKPPISAVQPVESAPAETPKAASFQVNFIKSGKSISTAGDATLLELAEAN
                     GVEIEYACRSGVCGTCKSKLNKGKVEMSENELSDEEKADGYIYPCVAHPVSDVEIEA"
     gene            complement(8384..10021)
                     /gene="hcp"
                     /locus_tag="PLG98_08350"
                     /gene_synonym="priS"
     CDS             complement(8384..10021)
                     /gene="hcp"
                     /locus_tag="PLG98_08350"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOE33306.1"
                     /translation="MFCYQCQETVKNQGCTVKGVCGKPDATAALQDILIYVCKGIAVA
                     AEKLNAVDKKASRYVCEALFTTITNVAWDDGRIIDKIEEGLKIRDEVNAKAASSLSGA
                     QPDCVTWTPKSREEIMAKAMSEEVRINAASNEDVTSLRSLLIFGLKGIAAYAEHAAVL
                     GYEKDDVYAFIMEALASTTKDLSVEAMIAMVMKAGESAVNTMALLDEANTKTYGNPEI
                     TEVNIGVRNNPGILISGHDLKDMEELLRQTEGAGVDVYTHGEMLPANYYPAFKKYSHF
                     VGNYGSSWWHQNEEFETFNGPIILTTNCLIPIKKDNTYLNRLFTTSVVNYPGAKHIAD
                     RPAGGAKDFSDVIAMAKTCPPPKEIETGKIVGGFAHNQVLALADKVVTAVKSGAIKRF
                     VVMAGCDGRQKARGYFTEVAEALPKDTVILTAGCAKYRYNKLPLGDIGGIPRVLDAGQ
                     CNDSYSLAVIALKLKEVFGLKDINELPISFDIAWYEQKAVAVLLALLYLNVKGIRLGP
                     TLPAFLSPTVAKVLVEKFDIKPIGTVADDVAAMMAGK"
     gene            10142..10831
                     /locus_tag="PLG98_08355"
     CDS             10142..10831
                     /locus_tag="PLG98_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE33307.1"
                     /translation="MKKARDILIRSQLFGGLPQEYITEIEKIAVDKHYNKGDIIFYDG
                     DNGVGFYLVAAGSVNVYKLSPDGKEQIFHIVKEGETIGAVPVFSGKSFPANARALSKT
                     HLLFFDRIKFIELISGKPDLTMNILALLSMRLREFTVQVENLSLKEIPGRLASYLLYL
                     AQEQGNKHFVKLNISKLQLSNILGTVPESLSRALSNMKAKKLIEEEDAGIRLLNRAEL
                     EDLAERGKTSN"
     gene            complement(11073..11513)
                     /locus_tag="PLG98_08360"
     CDS             complement(11073..11513)
                     /locus_tag="PLG98_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33308.1"
                     /translation="MKKIDKTLLDIRSFEGEGYQPLIDCDQWRVAILRYCDELLPERI
                     FAVQRHNETDEVFVLLEGRCILFLGEGKDTVTSITAVDMQPRKIYNVKRGVWHSHTLD
                     RDTSVLIVENRDTTVTNSPKIILNDDHRRKIVDITRELWLEENQ"
     gene            complement(11710..13269)
                     /locus_tag="PLG98_08365"
     CDS             complement(11710..13269)
                     /locus_tag="PLG98_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33309.1"
                     /translation="MKKYVLVIVVLFLSFLHVGMLYAEKKADFGGAGRYAAVGFAIDG
                     KGYVGTGYDGSTAYKDFWEYDPVANVWTQKADFGGGKRYAAAGFVIDGKGYVGTGYDD
                     SINYKDFWAYDPVANVWTRKTDFGGVIRNGAVGFAVGDIGYIGTGGDGSTNYKDFWAY
                     DPMADAWTQKTEFGGEERIFAVGFSIGSKGYVGTGLNGATKFKDFWVYDPATDAWVQK
                     INAGETARYGAVGFPMDDKGYVGMGGDGATAYKDFGEYNPATDTWTPKVDFEGAARGL
                     AVGFSIGSKGYIGLGLSETAKHKDFWQYDPNLDKTPDPFTFVDQTEVDWNRTITSNTI
                     TVSGVDASVVISITGGTYSINGGDYTSEDGTVNNGDTVTVRQTSSSVFHTTTDAELTI
                     GDISDTFSVTTKYLYDAGDNGPCFIATAAFGSPMAGQVEILRWFRDNYLLTNDAGRKF
                     VAWYYRYGFLAANFIKDKPLIRATVRWALYPLIGFALLLMSGTWPFVMIGILLSTFIC
                     LRVRSGKSKPV"
     gene            complement(13318..13710)
                     /locus_tag="PLG98_08370"
     CDS             complement(13318..13710)
                     /locus_tag="PLG98_08370"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HOE33310.1"
                     /translation="MGKRKTLLWVFSMMVISALVVVFQTELSVCKEAVNKETGQETKV
                     RRGPYADAKITFRIIPSASNTYGYDILMNGKTFIHQPNIPALPGNKGFATPDKAAKVA
                     SLVAAKIRNNDMPPVVTMDDLNRMGVLK"
     gene            13911..14255
                     /locus_tag="PLG98_08375"
     CDS             13911..14255
                     /locus_tag="PLG98_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33311.1"
                     /translation="MPDLLIKVALYQSNYLAAGAASGAGAASGAGAGAASGAGAGAAS
                     GAGASTAGASAGAVSSAFLQPTTANDSEATNSIATKIANIFFINREPPFKDFRDLVYG
                     PSFCMGDTLYSI"
BASE COUNT         3657 a         3917 c         3552 g         3648 t
ORIGIN      
        1 ccagcacaaa caggatcatg cttccatatt gtccgggcgt gagcgcccca taccgtatat
       61 ccccgttttg tgcagcgtcg agacggagaa aatccagaaa aaatctcacc ggcgtataga
      121 gaatcaaaac cgcggcggta taacttcccg gtctggcctg caggcggtgc agaacaagcg
      181 tcacggggaa aaggacaagc aaggtcagca ggaattcata aaacccgaga ttatgccgtg
      241 cgccgcccgg ctgttgaaag gccaggaaaa aatcgcttaa ttggccggga tgatcaaaaa
      301 taatcgtgca gccgagtctg ccgaaaatcc atcccagaac cagtccctga aggatgcatt
      361 cggcaaaaag cagaggcgag cggatttttc ttcgataaac gaaaaacatg aacccgaaga
      421 gtccgccgaa aaagcctccg aaagaactga gccctttcca gaacttcagc aggtaaacag
      481 gcccctgccg cataatctga tcgggatgat aaaaaacggc ttcggcaatg tgtgcaaaaa
      541 agaatgccga cagcaacgta tagagcaggg catcatccat gtcttcttcg ggaatacccg
      601 cctgcttcgc ccgatgcaac gccagcgcgc gaccggtcag aatgcctgta gcggccagca
      661 caccgaaagg actgatgtgc aaaacaccga tacggaataa ttcgacggtt ttgatatcgg
      721 gcagcaaaag tctatgcctc cattcttttc atcagggaat cccagccatc ccgggtttga
      781 attttcaaaa aagcgggaaa tcgcctgctg gttgtagcac atgttttatc ggagttcagt
      841 tatttttttt attccccaaa ccgttcggga catccgtaac cggaaggtcc ggatttttca
      901 ttgacaggcc ttttcgcttt tcctatactt tctgaaagtc attttcatac gggaagcaag
      961 gcaagccgcg tccccggtct tttgagcaaa ggactcttaa cccaaccccg gcaagaggat
     1021 ccaacatgaa agttctcgtc atcaacagcg gcagttcatc attaaaatat gaggtttttg
     1081 acctcaacgc cgagcaatcg ctcatgagcg gcgcggtctc gcgcatcggc acgaaagagg
     1141 cagatcatgc gtgcgcctgc ggtgacaaaa aacagttgac gaaaattgag gcgccggatc
     1201 atcgcgccgc gttggacttt gtccttcagg caataaccgg catggaagga acgccgatta
     1261 agaacctgga ggaaatcgac gccgtggcgc accgcatcgg tcacggcggc tcgctgtacc
     1321 gcggccccgt ggtcattgat cagagcgtga ttgacgaaat ccggcggctt attcccctga
     1381 tgccgctgca tcatcccgcc atgctggctg gtatcgatgc ctgccaaaaa gcgctgccgg
     1441 atatcccgca tgtggccgtt ttcgacacct cgtttcacag cgccattccg gacgaagccg
     1501 ccatttacgg cctgcctcat gaatttttca ccgccggcgt aaggaaattc ggctttcacg
     1561 gaaactcgca tgaatatgtc gcctcaaagg ctgccgagta tctggagact ccgatgagac
     1621 gcctgaatct catttcctgc cacctgggca acggggccag cgtgtgcgct attcagcggg
     1681 gacattccat cgacacctcc atgggcttca gcccgctgga ggggctgatc atgggcacgc
     1741 gcatcggcga tcttgatccg gggatcatcc cctatctgat gcgtcagcat catctggaca
     1801 tcgatgcatt ggacaaaatg ctgaacaaca aaagcgggct tctgggtatt tccggcgtga
     1861 gttccgatat gcgggaaatt atcgccgcag cagatgccga caacgcccgc gcgcttctgg
     1921 ccattaaagc gttctgttac cgcatcaaga aatacatcgg agcgtatcac ggcgttctgg
     1981 gcggagccga tgcgttgatt ttcaccggcg gtatcggcga gaacaaccgc agcatccgtt
     2041 cccgctgtgt gcagggcctg gagaaactgg gatttgccat cgataaagtc cgcaacgatc
     2101 gttgcgaagt ccatgcggaa aaaccggtct gcgatatcag cgcgcgctac gcgaataccc
     2161 agattctggt catcgcgacg gacgaggaac tgatgatcgc caggcagtgc gcccgcgcgc
     2221 tggattatac aagttccatc aaacatcacc tgctggccac ggaccggcgt cccatccgcg
     2281 tttcggtctc cgtgcgccac gcgcatttaa gcaaatcgga tgtggaagcg ctgttcggac
     2341 cgggatatgt tctgacatcc aaatcgcccc tctacattga cagcgaattt gccgccaacg
     2401 aaaccgtcga tctcgtcggg ccccgcggcc gggtcaacaa cgtccgcatc atcgggccgg
     2461 aacgactcaa aacgcaggtg gaaatatccc gtacggagga atttcagctc ggcatcgatg
     2521 ctcccgtgcg tgagtcgggc gatctggacg gtacgcccgg catcattctg gagggaccgg
     2581 cgggacggat cgaaattccc gaaggcgtca tctgcgcgat gcggcacatc cacatgacac
     2641 cggaagacgc cgaactatac ggcgtcaagg acaaagatat cgtcatggtg cgcatcgaag
     2701 gcgaccgcga acttattttc ggcgatgtgc tggtacgcgt caaacccaat tataaactgg
     2761 aaatgcatat tgatacggac gaggccaacg cggcggaact gccgccggta tccgaaggtt
     2821 atctggtacg catcgagtcg cgcaattaga tggaagcccc ccaaaggact ggtacattgg
     2881 acggcagcgc gattccctgt ttgccagagt gttcaattta tgataagcac ttaaagctct
     2941 ccctgaaaag agagcttttt taatgagacc caaatttcag aaacgtagtg agacttgcta
     3001 aaatcgagcg aagcgaagat tgaagcatag gtcgaactat cccgcaaagc ggtggaagtg
     3061 tactgaaatt tgatggtcga attatccccg aggcgaataa cctaaaggtc actataggcc
     3121 gagcgggata attgtgggtc taattccccg acccttgggg cgcaagtttc tttggatact
     3181 tcgctgcttg cggcggtgtt gtttaatcca gaattttcgc ggcaaacaac cggctttgat
     3241 gactaaaaaa aaactgaaaa ttatgctgat caatcctccc aactgcggga ggagcatccc
     3301 cgaagaagaa tacggaatca catcgctgaa acagattttt aagggagaac cttttaatct
     3361 ggaagtgctg gccggtccgc tgatcgatca tgacgtgatg attatggatt tgaaatgcga
     3421 accggacggc gccctctgga aggccttcga tgattttcag cccgatgtag tcggcctgac
     3481 gtcgctcacc tgcgaagcca atacggttat cggcattgcc cgcggagtaa aagaaaaaag
     3541 caatgccatc gtcgtgatcg gcggaaatca cgccacctac aacccggaat attttaaccg
     3601 tgctgacttc gattacattg ccgtcgggct ggccaagaag agtttccgcg acctgattga
     3661 tcatctggct ttcggccgtg aaccaacggg tctggccggc attgccagat gcgcgcccgg
     3721 cgcgccgctg gcctatacaa aacgatcgtt cgatgaaaag gatttaatgg atgatatccc
     3781 accccgctac gatctggtcg cccaatacag agatcgctat ttcctggaac atctcggtct
     3841 gacgatgggt tttgttatta ccgcctacgg ctgcacgcac cggtgttctt tctgcaccat
     3901 tccggaaatg acgggaggaa aatatttgaa ccactccgcc ggggccgtcc ttcgcgatat
     3961 tcaattgctc ggcgatattc ccttcatcag aatggtggac gccaacacgt tcggtaatct
     4021 gtctttatcg cttgaccttt acgcaaaaat catgcaatcc ggtatccgga aaaagttttt
     4081 tgccgatgtc cgggccgaca caatcgtcaa aaatccggat ctgatgaaaa aatggaaaga
     4141 ggccggactt tacgcggtgg tgatcggttt tgaggatttt caggacgctc gccttgacgg
     4201 ctataacaaa aaatatcagg gacgcgtcat ccatgaggcc attgaaattc tgcatgacct
     4261 gggcatcatc atggtcggcg atttcatcgc ctcgcccgat tatgacgaaa gcgatttcga
     4321 aaaactggag gcgtttattg tcgcccatca aatccagatt ccggtgattt cggttttaac
     4381 gcccattccg ggcacgccgc tttatgaaaa gatgaaaaaa gatattgtca ctgacgatct
     4441 ggattactat acgtttacga acgccgtaac gaaaacaaaa atgccggaag cccggttcta
     4501 taaaacattt gccgatatgg tcaaaagact gcatcgtcag ccgcacaaaa ccacttaaag
     4561 aaaggacttt atggacgttt acatcaacga cattgccgcg tttctgccca acgatccggt
     4621 gactaacgac gaaatggaaa cggtgctggg cgccgtgcac aatattccct cccggataaa
     4681 aaacagaatt ttgaaaaata acggcatcga aaaacgctat tacgccattg accgcaaaac
     4741 gggcgaactc aactacacca atgcccggct gaccgccgaa gccgtacgca gattgaaacc
     4801 ctatgaaggc ttttcggtca acgacatcca gatgctctgt tgcggaacct ccactcccga
     4861 cgtgcttctc cccggtcacg gtctgatggt gcagggcgaa ctgggcatcc cgtcgtgcga
     4921 agtcatcaca accgcgggca tctgcctgtg cggcatcacg tcattcaaga ccgcttatgc
     4981 caacgtcgct ctggggctga gcgacaacgc cgtgtctacc ggctcggaac tggcatcatc
     5041 cttgatcaag tcgaattttt ttgagcatct ccatggcgat cgcgatttca agaatcatcc
     5101 cgtcctgccc tttgagtcgg atttcctgcg ctggatgctc tccgacgcgg ccggcgccgt
     5161 ttttatgtcc ggcaaaaaaa accgaaacgg tctttcgttg cgcgtggact ggatcgaaaa
     5221 cattgcttat gcgggacaga tgaaaacctg catgtacgcc ggcgcgcaca aaaaagaaga
     5281 cggcacagtg atcggctggc gtcagcttca ttcgctggaa gaggcgctga gcagtaatta
     5341 tttcgctctc aagcaggaca ccgaactgct caaccgggaa atcgtccgcg tgatgatcga
     5401 tctgggcctt tcccataccg ttgaacgaag aaaaataaaa ccggatgaca tagactggta
     5461 ccttcctcat tattcatcgg atttcttccg ggaaaaaatc tttaacgcca tggaaggaat
     5521 cggtttcaga attccctttg aaaagtggtt taccaacctg gcctacaagg gcaacacggg
     5581 gtctgcttcc atttacgtga tcatggaaga attgttccag tcgggtcaat tgaagagcgg
     5641 acagaaactt ctctgcttta taccggaaag cggccggttt tccgtcgcct atgttctgct
     5701 gacggtggtc tgagtcctgt atcaattttt attcagaaaa tgttttcccg ccagaatggg
     5761 atggcgataa atcatgcgcg gaccggcata ccgcatcacc gctttgattt tttcccgcat
     5821 gtccggttta tagcagtgga cgggacattt tgagcatttg ggcttattcg tcttgagtgg
     5881 acatttttcc aggcgttgtc cgacgtaggt cagcaacgct tcgcagtcaa cgcaacgatg
     5941 atcccgactg tcatgatgac cgcggcagta aagatcaatc atcacactga cggttttaaa
     6001 ttcctgttcc attgtccttc atgaagactt taagatcttt cccctcaagc tcagcacgat
     6061 ttctttcatg ggaatctcct tgcctgccgc ctccatgcct tttttgacaa tcatcggcat
     6121 tcccgaacag cagggcacct ccatcaccac cacggtgacg ctcctgattc cggcggtttt
     6181 gaagatgtcg gcaaattttt gcacatactc cgggacatcg tcaaatttcg gacatcccat
     6241 catcaccact tttcccttca ttaaatcgcg atgaagggtg ggaaaggcga cggccgtgca
     6301 gtccgcaagg atcagcaggt cgccgccttt caggaagggg gcactggccg gaattaattt
     6361 gatctgaatc ggccagtggg tgagaacgga ttcttgttcc acattgtcga agtgtttcgg
     6421 cttattggcc tctgcgcagg cggagggttt gtcaaaaatc tgaatgtgag ttgacggaca
     6481 accgcaaggc atcgtctccg gttctttttc cttttttgcc agatgttttt tgacggcttc
     6541 ttcatcaaac ggagacgctt cgcgctcgac aattttcagc gcacccgtag ggcattcgcc
     6601 gatacaggca cccaggccgt cgcagaacgt ctccgatacc accttggcct tgccgtcgat
     6661 gatttgcaga gcgccctcgg cgcagccggt cacgcaattg ccgcaaccgt tacacaattc
     6721 ttcttcaatc tcgataatct tgcgtaatat tttcataatt tatgcctcta tttctacatc
     6781 cgacaccgga tgtgccacac aggggtagat gtatccgtcc gctttttcct catcggacag
     6841 ttcgttttca ctcatttcta ccttgccctt gttcagttta gatttgcatg tcccgcagac
     6901 gccggaccgg cacgcgtatt caatctccac accgttcgct tccgccagtt caagcaacgt
     6961 cgcatctccc gcggtcgata tggattttcc cgacttgatg aagttcacct gaaagcttgc
     7021 cgctttcggc gtctccgccg gtgcgctttc aaccggctga actgccgaga taggcggctt
     7081 tggaacgtcg cgcgcatcaa ccttctcccc tttggccacc cgcccttcgc caaaactttc
     7141 cgtgtgcaga tgggcaagat taaaaccgag taattttaaa ttttccttaa ccgcttccat
     7201 gaacggcgaa ggaccgcaca tgaaaatatg ccgctccaga atatccggcg tgaacagttt
     7261 cagcattttc tcgtcacagc ggccggtcag accagtccat gcatccgcgc cggtgacgcg
     7321 gctggtgcag gttataccca cgcggatgcc gctgtggcgc gacgacatcc agtcaagctc
     7381 gtttttgaag atgatctcgc gcggagactt ggcagatacc agcaggcgcg cgtccacatc
     7441 ggccgccgtg tccacgatcc agcggagcat ggacatgacc ggtgtgatcc cgctgcccgc
     7501 accgataaac agaatctttt gcgacgggta gttaaagcac gaaaacaatc ccgacggacc
     7561 gtgcacggca agatggtcgc cgagcttcac atggtcgtgc atccagttcg agaccaggcc
     7621 ctgcggtgtg cgcttgatcg tcatttccag gcagtgtgga cgcgaagggc tcgatgagat
     7681 cgtgtaagaa cggcgcacgt ccgtgccgtt gatgttcacc ttgatggtga cgaactgacc
     7741 gggtttgaag gaaaacagaa ccggcgacaa gccgaccagt cgaaatgtct tgacatcatc
     7801 cgtttcgtcg atgatatcgg cgatcttcag ttgaatatct ccttgcgtcc acagaggaat
     7861 ttcgtgatgt ctttcgacca aattttccag gagcggactg gctgacggag gcggttcaat
     7921 gtcgggcgtc acaaaacgcg gacgcgtctg gtcaggcgcg accggttgag attcaatttg
     7981 aaatctcaat atatactcac ccaattcggc aatatctcct gaattaaccg ctgtcggctg
     8041 cataggggcg atcttccggc cgttcagcaa cgtgccgttg aggctgttga gatccaccag
     8101 aaaccagcgg cccgcgtcct tctgaaacat ggcgtgaatg cgcgacaggg cggtatcttc
     8161 aagctgaata tccgcacccg cggcgcgtcc gatgatcata tgaccgccga gggttatggt
     8221 ttcaatcact tcattgccgc gggacacgat cactatattc tgttcgtttc tttctgacga
     8281 cacaggggaa ttcctctctt ttttcacgat tctctctttc tgctggtgac tctcccgaat
     8341 ttttcatccg ggagaatcac cgagtttaat gatcggccat cgattatttg ccggccatca
     8401 tagcggcaac atcatccgcg actgtgccga tcggtttgat atcgaatttt tccaccagca
     8461 ccttcgcaac cgtgggtgac aagaaggccg gcagcgtcgg gcccaggcgg attcccttga
     8521 cgttgagata cagcagggcc aacagcacgg ccaccgcctt ctgctcgtac caggcgatat
     8581 caaaggaaat cggcaattcg ttgatgtctt tgaggccgaa aacttccttc agcttcaacg
     8641 cgatcaccgc cagcgagtag gagtcattgc actgtcctgc atcgagcact ctgggaatgc
     8701 cgccgatatc gcccagcggc aatttgttgt agcgatactt ggcgcagcct gcggtcagaa
     8761 tcacggtgtc tttgggcagc gcttcggcga cttcggtaaa gtagccgcga gctttttgtc
     8821 ttccatcgca accagccata acgacaaagc gtttgatcgc acccgatttc acggcggtga
     8881 cgaccttgtc ggcgagcgcc agcacctggt tatgcgcaaa tccaccgaca atcttgccgg
     8941 tttcaatttc cttgggcggg gggcaggtct tcgccatagc gatgacatcc gagaaatcct
     9001 ttgcgccgcc tgcgggccta tccgcgatat gtttggctcc gggatagttg acaacgctgg
     9061 tcgtaaacag acggttcaaa tacgtattgt ctttttttat cggaatcaaa caattggtgg
     9121 tcagaataat cggaccgttg aaggtttcaa actcctcgtt ctgatgccac cacgacgagc
     9181 cgtagttgcc cacgaaatga ctgtattttt taaacgccgg ataatagttg gccggcagca
     9241 tctcgccgtg cgtatacaca tccacgccgg caccttccgt ttgcctgagc aactcttcca
     9301 tatccttcag gtcgtggcct gaaatcagaa tgccgggatt attgcgaacg ccaatgttga
     9361 cttccgtaat ttcgggattc ccgtaggttt tggtattggc ctcatccaga agcgccatgg
     9421 tgttcacagc cgactcaccg gctttcatga ccatggcaat cattgcctcc acggaaagat
     9481 cttttgtggt ggatgccagc gcttccatga tgaaggcata gacatcatct ttctcatagc
     9541 ccaaaacggc ggcatgttcc gcgtaggcag caatgccctt gagtccgaaa atgagcaggg
     9601 atcgcaggga ggttacgtcc tcattcgacg cggcattgat gcgcacttcc tccgacatgg
     9661 ccttagccat aatctcttct ctacttttcg gtgtccatgt gacacagtca ggctgtgcgc
     9721 ccgacaggga ggaagccgcc tttgcgttga cctcgtcacg gatcttcagt ccttcttcaa
     9781 ttttatcaat tatgcgacca tcgtcccagg ctacgttggt aatggtggtg aaaagcgcct
     9841 cgcaaacata acgacttgct tttttatcca ccgcatttaa tttttcagcg gcgaccgcaa
     9901 tacccttgca cacataaatg agaatatcct gaagggccgc tgttgcatcg ggcttgccgc
     9961 aaacaccttt gaccgtgcat ccctgatttt taaccgtttc ctgacattga tagcaaaaca
    10021 tatgaaacct ccctgtaaaa gattatttgt ctttttgttg gtgagtatag ttaaaaaact
    10081 aacgcgtttc cttgacttat gttaagaaaa cgatatattt tttctccgaa ggacgtaacc
    10141 tatgaaaaaa gcgcgggata ttctaatcag aagccagctt ttcggtggat tgcctcaaga
    10201 gtatatcaca gaaatcgaaa aaattgcagt cgataaacat tacaataaag gcgatattat
    10261 tttttatgat ggagacaacg gtgtcggatt ttatctagtt gccgccggta gcgtcaatgt
    10321 ttacaaactc tctcccgacg gcaaagaaca gattttccat attgtaaagg agggcgaaac
    10381 catcggcgcg gtacccgttt tttccggcaa gtcttttccc gccaatgccc gggcgctttc
    10441 caaaacgcat cttttatttt tcgaccggat aaaattcatt gagctcattt ccggcaagcc
    10501 ggatctcacc atgaatattc tggcgctgct ttccatgcgc ttgcgtgaat tcaccgtcca
    10561 ggtggaaaat ctgtccctca aggaaatacc cggccgcctc gcctcctatc tgctttatct
    10621 tgcgcaggaa cagggcaaca agcatttcgt caaacttaac atttccaaat tacaactttc
    10681 caacattctg gggacggtgc cggaatcact ttccagagcc ttgagcaata tgaaggcgaa
    10741 aaagcttatc gaggaagaag atgccggtat ccgattgctc aaccgagccg agttggaaga
    10801 tctggccgaa agagggaaga cctcaaatta atcgaacggc aagagaagat ccattggttg
    10861 agaaatccct gcctgcgttg atgcgcattg agaaatgaat ctgaccacag cagcgcccat
    10921 tctgcgtgat cgttttaaaa ccgttcagaa aataacgaac ctcgcggcga tgaaagcatc
    10981 gcggtaacgg acggtttgca aaccgcttaa aaatttaccg gcaggttaga tcggactttc
    11041 tccacatcgt ccttttttca ttgctcaaac gattattgat tttcttcaag ccacaactcg
    11101 cgtgtgatat ccacaatttt ccggcgatgg tcatcattta gaatgatttt cggcgagttt
    11161 gtaactgttg tgtcgcgatt ttccacaatc agcacggagg tatccctatc cagcgtatga
    11221 ctgtgccaga cgccgcgctt aacgttgtaa attttccgag gctgcatatc gacggctgta
    11281 atcgacgtga ccgtatcttt tccttctccc agaaaaagga tgcagcgccc ttccaaaagc
    11341 acaaacacct catcggtttc attgtgccgc tgcacggcga aaatgcgttc aggcagcagt
    11401 tcatcacaat aacgcagaat cgccacacgc cattgatcgc aatcaatcag cggctggtag
    11461 ccctcacctt caaaactgcg aatatccagc agtgttttat caatcttttt cacttacgat
    11521 ctccatttta taatatcggt ttttctagca tatcttccgt tttcgtgcag ccgttttttg
    11581 atcattccag aaaatggttc tatcaaaaat ccacgaccgg ttgacaggca gggttgttga
    11641 cggattaaag aaaacagctc cccaactttg aaatgttcag gagctgtcat gttgatccaa
    11701 atcgatcttt taaaccggtt ttgatttgcc tgatcgtacc cgcagacaaa tgaaagtcga
    11761 caaaagtatc ccgatcatca caaaaggcca cgtgccggac atcagcagaa gggcaaatcc
    11821 gattaagggg taaagtgccc accgcactgt cgccctgatc agaggtttat ctttgataaa
    11881 attggctgcc agaaaaccgt agcggtaata ccaggcaaca aactttcgtc cggcatcatt
    11941 ggtcagcaga taattgtctc tgaaccagcg aaggatttca acctggccgg ccatgggcga
    12001 tccgaatgcc gctgtcgcaa taaaacaggg cccgttgtcg cctgcatcat acaggtattt
    12061 cgtggtcaca ctgaaagtat cggagatgtc accgatagtc agttcggcat cggtggtcgt
    12121 atgaaaaacg gaagacgatg tctgcctgac cgtcacggta tccccgttat tgacagtccc
    12181 atcttcactg gtgtaatcgc cgccgttgat ggagtatgtg cctcctgtaa tggagatgac
    12241 aacgcttgca tcaacgccgg atacggtaat ggtgttggat gtaatcgtgc ggttccagtc
    12301 aacctccgtt tgatcgacaa acgtaaacgg atcaggggtt ttatcaaggt tgggatcgta
    12361 ctgccaaaag tctttgtgct tcgccgtttc gctcaatcct aacccgatgt agcctttgct
    12421 gccgatggaa aaaccgacgg ctaaacctct tgccgctcct tcgaaatcaa cttttggagt
    12481 ccaggtgtcg gtggccggat tgtactctcc aaaatcttta taggctgtcg cgccgtcacc
    12541 tcccataccg acatacccct tatcgtccat gggaaaaccg accgcaccat accgggctgt
    12601 ttcgccggca tttatctttt gcacccaggc atcggtcgcc ggatcgtata cccaaaaatc
    12661 tttaaatttt gtcgcaccat tcaatcctgt accgacgtac cctttgctgc cgatggaaaa
    12721 accgacggca aaaattctct cttctcctcc aaattcagtc ttttgcgtcc aggcatcggc
    12781 catcggatcg tatgcccaaa aatccttgta atttgtcgaa ccgtcacctc cggtaccgat
    12841 atatccaata tcgccaacgg caaacccgac ggcaccattt ctgattacgc cgccgaaatc
    12901 cgttttccgg gtccagacat tagcaaccgg atcgtatgcc caaaaatcct tgtagtttat
    12961 tgaatcgtca taccctgtac cgacatatcc cttgccgtcg ataacaaacc ccgcagccgc
    13021 ataccgcttt ccaccgccga aatccgcctt ctgggtccag acattggcaa ccggatcata
    13081 ttcccaaaaa tctttatagg ccgttgaacc atcatatccc gtgccgacat accctttgcc
    13141 gtcgatggca aaaccgacag ctgcataccg gcctgctccg ccgaaatccg ccttcttttc
    13201 cgcgtaaagc ataccgacat gaagaaaact taagaataag accacgatga ccagaacata
    13261 ttttttcata cggaatctct cctgctggta tcttcaccca tcatctcttg atggatgtta
    13321 tttcagtacc cccatcctgt ttaaatcatc catcgtcacg acgggcggca tatcgttgtt
    13381 tctgatttta gccgccacca atgaagccac ttttgcggct ttgtccgggg ttgcaaatcc
    13441 tttgtttccc ggcaaagccg gtatattcgg ctgatggata aaagttttgc cgttcatcaa
    13501 aatatcatat ccatacgtat tactggccga aggaataatc ctgaaggtta tttttgcatc
    13561 agcataaggg cctctccgga ctttagtttc ctgacccgtt tctttgttga cagcctcttt
    13621 acaaacggac agctccgttt gaaaaacgac aaccagtgca gatataacca tcatggaaaa
    13681 aacccaaagc agggtttttc tttttcccat cggaaacgat cctcattcag caagaacttt
    13741 atgactgtaa ttttttttgc aagtcgatga aatataggca atctgtcccg gactgtcaag
    13801 gaaacattga ccgtcacaaa aatcatctgc ggcagtcatg attacgtttg cagacacgtc
    13861 acgcctttta aaaacaggta aaaataataa atcggacatt cacagaatga atgcccgatt
    13921 tattgataaa ggttgctttg tatcaaagca attatttggc agcaggagcg gcttcaggag
    13981 ccggagcagc ttcaggtgca ggtgccggag cggcttcagg tgcaggtgcc ggagcggctt
    14041 caggtgcagg tgcttcaact gccggagcct ctgcaggtgc tgtctcctca gcttttttgc
    14101 aaccaacgac tgccaatgac agtgaggcaa caaacagcat agcaacaaaa atagcaaata
    14161 tttttttcat caaccgtgaa cctcctttca aagattttag ggacctggtt tatggaccaa
    14221 gtttttgtat gggagatacg ctatactcta tttaaaagtt ttgtcaagag tcaaaaataa
    14281 aaaggctttt tcatgaacga tcctcctttt ttccggtttc cctgaaaatc atatatggat
    14341 tcgtttaaac aaacatcctt ttttaagaaa ataccgtaaa ggtcttgaca agcaacaccg
    14401 ttttatgcgt tagtgtgctt aatcattaac ggctcaaaag ccatccagtt taatgcctgc
    14461 tcgagatcgc ctctcagcag gcattatttt attgttcgga ttgatccgtt catcctgcgc
    14521 taggccgctc tccttgagct tcccgcacat catgcattga gaaatttttt ataccataaa
    14581 agagtggcac cctgtcattc ccggcattat catttcctga ccgacgatat ttgtacatgc
    14641 ccgggtaatt gacagaaccg gtcaaatcat gttaaagcgc ggaaaattct gcgaaacaag
    14701 gttgcccctg tagctcagaa ggatagagca acggattcct aatccgtgtg ccgcgcgttc
    14761 gactcgcgcc aggg
//
LOCUS       DAOSME010000054        14689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4813_length_14689_cov_30.225229, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000054 DAOSME010000000
VERSION     DAOSME010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14689
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4813_length_14689_cov_30.225229"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PLG98_08380"
     CDS             <1..1138
                     /locus_tag="PLG98_08380"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HOE33312.1"
                     /translation="GVVTKLQTTIGRLIENIVWAQQSIQTGLNFWGRNLLSDHEAKKL
                     REKLDATKAFLESLQAFNTTGKLKNFRYDAADVKAHGEGLRALSEMKSLQEIVNDFGS
                     TAGYLSQAEAVLHSDHEWIDRMKAIRADMFTAIENFKTKGPGTLRQTLSAQLTKLKRD
                     YIAVYLALHAKARLGLKEDKRKVQLISDERLQILQKLATIDLMPRQHLSDFQSRLAGQ
                     KSCFTLIEQELNASPVCPHCAFKPGMESSYGRMPASTILDGFDDELDKMLANWTQTLL
                     ANLEDPTTKENINLLKPPARKLIDGFIKKRSLPDDLSQDFIHAMKEVLSGLTKVAVTV
                     EQIRNALLSGGSPATPSEMRKRFDEYLEELTKGKESGKVRIVLE"
     gene            1148..2266
                     /locus_tag="PLG98_08385"
     CDS             1148..2266
                     /locus_tag="PLG98_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE33313.1"
                     /translation="MDANKHEWNFENLVGMIRQAHEELAAQAGHAVNISLTLRNWMIG
                     CYIAEYELCGSDRANYGGNLLSNLAQSLTAFKISNCNRRQLYRYLRFYRLYPEIVGTL
                     SAQLKKLLPPEARAREKVGTASPQLWTPPDKIIHLLAYSHLELLVDLEDDLKRAFYEI
                     ECIRGNWSVRELKRQIDSLYYERSGLSKDKKELSKLAQKGAKAADPKLAIRDPYIFEF
                     LGLKPREVMSESHLEDQLLNKLQDFLLELGHGFCFEARQKRILIGDTHNFVDLVFYHR
                     ILKCHVLVELKLEHFSHENIGQLNTYVSWYKKNMMTEGDNPPIGILLCTQRDQTLVEY
                     ALAGMDNGLFVSKYQLELPKKEEMQQFIEEQIREREDN"
     gene            2316..2705
                     /locus_tag="PLG98_08390"
     CDS             2316..2705
                     /locus_tag="PLG98_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOE33314.1"
                     /translation="MATITRFEDIEAWQKSRELTREVYAASNTGLFAKDFGLRDQIRR
                     ATVSIMSNIAEGFERGGDKEFVQFIAQAKGSSAEVRAQLYVALDQGYIDKPTFDRLAE
                     MSMHINRMLAGFMKYLRSSEFKGSKYK"
     gene            2849..5671
                     /locus_tag="PLG98_08395"
     CDS             2849..5671
                     /locus_tag="PLG98_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOE33315.1"
                     /translation="MDSLQAEQSALDFRSKTLDSKNSPVTCLGMTFPSDDERRKYFTE
                     KLREFLADPEFRKIEGFPIGADEDILALSDPPYYTACPNPWIADFIKHYGKPYDPNVP
                     YSREPFAADVSEGKNDPIYNAHSYHTKVPHKAIMRYILHYTEPGDVVFDGFCGTGMTG
                     VAAQMCGDRAVVESLGYKVENDGTVSQQETDENGKTIWKPFSRLGARRAVLNDLSPAA
                     TFIAYNYNTTVNVQAFEREAKRILKEVEAECGWMYETLHTDGKTKGKINYTVWSDVFV
                     CPECTREVIFWEAAVDKKAGAVKDEFSCPHCKANLTKRKMDRAWGTQFDKVINQTIRQ
                     AKQVPVLINYTVGGKRAEKTPDKSDLALIAKIENSDIPYWFPTTTIPKGDKTGEPLRI
                     GITHVHHFYTKRNLWVLAALWQRFTNYPMGKLAITSVLIKTASLLHNIGFKNGKINLA
                     GALPNALFIPSNIAERNIFELVQGKVDDFIRANLHRIQSRQIISTLSLSAENQNSNIY
                     SSADYIFIDPPFGSNLFYSELSFIWEAWLGVMTNNKSEAIESHSQNKTLDDYRILMTF
                     CFRQAYRFLRPGRWMTVEFSNTGAAVWNSIQTALSDAGFVVGNVAALHKGQGTFNSQT
                     NPTSVKQDLVISAYKPNGGFEERFQREASTEEGVWDFVRTHLKYLPVVKRGATATHLI
                     AVTERDPRILYDQMVAYYVRKGYPVPLNSAEFQEGLSQRFSYRDGMYFLPDQVAEYDK
                     KKMTAGGMEQMSLFVSDESSAIAWLRQLLKDKPQSFQDIHPHFIKELGGWQKYEKPLE
                     LSELLGQNFFCYDGKGDIPSQIHSYLSTNFKELRNLPKDDAGLRAKAKDRWYVPDPNK
                     AGDLEKLRERALLREFEDYRTTSQKRLKIFRLEAVRAGFKKAWQERDYATIITVARKV
                     PENVLQEDPKLLMWYDQALTRSGEES"
     gene            5694..7598
                     /locus_tag="PLG98_08400"
     CDS             5694..7598
                     /locus_tag="PLG98_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HOE33316.1"
                     /translation="MITKWTLSNFKSVSKETELSFAPLTLFAGPNSSGKSTILQSLLL
                     VSQTLAHKVSSRSVVLNGLITRLGQFDDLKSHESDYDEITIGWECRPLEGSQASTLEN
                     SRLPSHFMIYGRHNDAIKAITCKLSFDANPGSPDRELYQLQPRLFLFTISAVVNSDDN
                     ADRRHELTVSRSSAESTKIDNLNAEVPNDVARASLEFNVDIDNDSLEELREEFKTAEP
                     VGCMLRHFLPDRLSIRINAIKEVARIIQLALTDDGPRIRRSLFMKRELVIPYPLIKEL
                     QNRLGEKYAVLFEKQTNLFDGNEGMAVREWMERLRKLPFDRRRELRRTLAEWSDLAEI
                     IERELRDASQKEDKEYLLLPLLLPADLMNASSYLGAMFSNAVKYLGPLRDEPKPLYPL
                     TAHADPTDVGFQGEMTAAVLNLHRERKIRYISSSAFAKTPFLPTPVTRSLESAVTDWL
                     QYLGVAETAYSIDKGKLGHELKVKLTENGPVHDLTHVGVGVSQVLPIVVMSLLADRDT
                     TLIFEQPELHLHPKVQTRLADFFLSIVLLGKQCLIETHSEYLINRLRYRAAAEDENNR
                     VNDNLKIYFVEKENGSSNFREVKVNEYGAILEWPEGFFDQSQREAEDILRAAMTKKKK
                     KQKRESKDNV"
     gene            7591..8664
                     /locus_tag="PLG98_08405"
     CDS             7591..8664
                     /locus_tag="PLG98_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33317.1"
                     /translation="MSEAFQLAIDPYILCLPNPCNSLEEIESFVDTLLGWSSTLQQKN
                     SNILISDAARIALLEDGEYPHQHRLRDLMRIHKCEVADPETVGRLTQSLLDRTPSLEA
                     FYGVNSILIDPAKSSIMPDAFFKRLKDNTRNALIEMLVIVSAEQNADCSHIEKHIFLA
                     TSDDLIDKDNDLTEIECVSELHDWQWLTEPLHDLPTMPATIEGKLPLVFSHYSFLHQL
                     GIWHVWNNASDEQAAIDAINLCIEEIVNTGATGISKINFRLGTYFLESVRAQSFGSRS
                     DYARVLIESCARITLDIPKNPVKPFRESANSMRQKRRHDGALAFRTHLTKKGVGFRLM
                     LWKLSDGTIEFANVGCKGELVIL"
     gene            8661..11546
                     /locus_tag="PLG98_08410"
     CDS             8661..11546
                     /locus_tag="PLG98_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE33318.1"
                     /translation="MMIENLHLGVWLWAIDHSQPCQVIETQTLWGETMCRVWMPGRDT
                     VVRIPASQLISLEKADSDSPHNIAYAAAAARVADALTQDILLAPIASSVIPLPHQIRA
                     LSRAIANDQVRYLLADEVGLGKTIEAGLIFRELKLRGLVKRALVIAPKGLVNQWVAEM
                     RLHFNEHFQLVLPDDIKTLSRISVVQSQKSNVQGQDYKSIRPWTSDRGLWTSGKGPWT
                     LCDQVVVPMDSIKPLDKRRGWSAAQVGEYNRERFEDLIAAGWDLVIVDEAHRLGGSTD
                     QVARFKLGQGLAEAAPYFLLLSATPHQGKTDAFHRLVSLIDAQEFPDVGSVTRARVSP
                     YVIRTEKKRAIDAEGKPLFKSRRTELFAVSWGKRHREQQLLYEAVTDYVREGYNQAMR
                     EKRSYIGFLMILMQRLVVSSTSAIRTTLEKRLNILASPQEQLSLFPAASEEEWTDLDG
                     QEQMEILLRTRLKALKNERAEVKLLLEAAARCEQIGPDAKADALLEWLYRLQSEEGDP
                     ELKALIFTEFVPTQEMLRRFLVERGFSVVCLNGSMDMDERKRVQDAFAKDVRILISTD
                     AGGEGLNLQFCHAVINYDIPWNPMRLEQRIGRVDRIGQSHVVRAVNFVFEGSVEHRVR
                     EVLEQKLAIIFEEFGIDKTGDVLDSGQAGKMFDEMYIEAIRNPDKIEDSVESVVTRMQ
                     EQVREAQNTVSVLGKAEDLQPSEAQRLLGHPLPHWVERMTVSYLQANGGRAERKSQGW
                     KLTWPDGQYDEDVVFTGKEAERFPAARHFTLEESKIRGLAMRLPRFVSGQPIPVVSLP
                     SISQEISGLWSLWQIAISTMGWNRRRIMPLFLADSGAVLMPTARHIWNTLLVEKIQTL
                     SVVDSSASLTSFNELQKVAQEHGKAIYDDLLQEHRTRMIREREKGEYAFAARRRMIGR
                     IGLSQVRNHRLALLNQEEKIFNEQLDQKNRIYPDMSPLLVIRVEGNH"
     gene            11546..13552
                     /gene="pglZ"
                     /locus_tag="PLG98_08415"
     CDS             11546..13552
                     /gene="pglZ"
                     /locus_tag="PLG98_08415"
                     /inference="COORDINATES: protein motif:HMM:NF033449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system phosphatase PglZ"
                     /protein_id="HOE33319.1"
                     /translation="MNSWRDHVLKEFTPKVSRLTLVSDPDGLLLEEGILEGIRKRGFD
                     LIPFEDHVAFRFAYESKYRSCWDRGEQTDLVVLLRSEIKDIDGLPYDLLQSGRKLFFN
                     VGDVFPKLSHPVIAALDRSYFEILYQAQKLHAPGELGENATKEFILRHVFEIAPELIR
                     QPSNLMQVLLRRHYRGQVIPPVLDERLIQLLQQNKQFVGWPLETIVKDREAFFTFLQE
                     RWPTFLDKEAVHVKKGVYEAHDEYHLALKGPVDLPFDHHDIRIYIDNLFMEGFLHPVQ
                     HEHADELSRSWVAVGIQTNAAEDCSRRLCKLAESLKAAIPAEDAKHSDWSHFARSWAK
                     LMCLVNEEPSSVPATTKSKIRALQTQIDIGFSTWLFKRYAGLINLPPAPPVMLHHIPR
                     FIARQMSDAAGLKVALLVVDGLSLDQWLVVKNILGANSSSFIFREKTVFAWIPSITSI
                     SRQAAFSGKIPAFFPDSIYTTEKESLLWSQFWTDQGLSQNEVVYIKGLGDGSLDMVSE
                     MISSPKARVVGLVVDKVDRIMHGMELGAAGMHNQVDQWARQLYLQKLVDLLLDQGFRV
                     YLTSDHGNIDAEGCGRPAEGVIADLRGERVRIYPDIALRTKIKGQFPAAIEWNNVGIP
                     DDCVALLAPHRQAFIETNRSAITHGGLSMEEIIVPFVQIERKIK"
     gene            13549..14301
                     /locus_tag="PLG98_08420"
     CDS             13549..14301
                     /locus_tag="PLG98_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33320.1"
                     /translation="MTSKRFEQIGLDRLVHLTWLEKTANLALAGNDAKTIKSILQDEL
                     AGAFRSANKEVRGSLDKTITILLKIWLMPPKDFETLRLLGLELMKQTPLNERTLIHWG
                     MIAAVYPFWSAVAAQIGRLLRLQGTVTAIQIQRRIREQYGERETVSRRARYIIRSFLD
                     WGVIQETNTKGIYKSENTLAVDDIRLIAWLVEASLYARSNGAVPLKDLIDSPALFPFR
                     IKSIRAETLIAVSPRLDFLRHGLDEDLIMLKQ"
     gene            14340..14531
                     /locus_tag="PLG98_08425"
     CDS             14340..14531
                     /locus_tag="PLG98_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33321.1"
                     /translation="MKNVEMKLEGDILTIKVDVTKEFGPSASGKTIIIASTEGNISVP
                     EKDDVKIGLNVYRKKPVAA"
BASE COUNT         3958 a         3338 c         3696 g         3697 t
ORIGIN      
        1 tggcgttgtc accaagctgc agaccaccat tgggcgactg attgaaaaca tcgtttgggc
       61 gcaacaaagt atccaaacag gcctgaactt ctggggacgc aacctgcttt ccgatcatga
      121 ggcgaagaaa ctgcgggaaa aactggatgc aacaaaagca tttctcgaat cgctgcaggc
      181 tttcaataca accggcaaac tcaagaattt ccgttatgat gcagccgacg tgaaggcgca
      241 tggtgaaggc cttcgtgccc tgtctgaaat gaagtcactc caggagatcg tcaatgactt
      301 cggatcaacc gccggctatc tgtcacaggc cgaagcggtt ttgcattcag accacgagtg
      361 gatagataga atgaaagcca ttcgggcgga tatgtttaca gccatagaaa attttaagac
      421 caaagggcca ggtacattgc ggcaaacctt aagcgctcag cttaccaagt tgaaaaggga
      481 ttatattgcc gtctatcttg ccctgcatgc caaggctcgt ttgggtttaa aagaagacaa
      541 gcgtaaagtt cagcttatca gcgacgagag gcttcagatt ctgcaaaagc tcgcgacaat
      601 tgatctcatg ccacgccagc atctgagcga ttttcaaagc cgcctggccg gtcagaaaag
      661 ttgttttaca ctcatcgaac aagagctgaa tgcttcgcct gtctgcccgc actgtgcttt
      721 caaaccggga atggaatcct cttatggccg tatgcctgct tccacgattt tggacggctt
      781 tgatgatgaa ctggataaaa tgctggcaaa ctggacgcaa accctgcttg ccaatttgga
      841 agatccaacc acaaaagaaa atatcaatct gctcaaaccg ccggcgcgta aactcatcga
      901 tggctttatc aagaaaagaa gcttgcccga tgatttgagc caggatttta ttcatgcaat
      961 gaaagaagtg ctttccggcc tcaccaaggt tgctgttacg gtggaacaga ttcgtaacgc
     1021 tctgttatca ggcggttctc ccgcaacgcc ttcggaaatg agaaaaaggt ttgatgaata
     1081 tcttgaagag ctcaccaagg gcaaagaatc cggcaaagtc cggattgtgc tggaatagga
     1141 actgcgaatg gacgcgaata aacacgaatg gaattttgaa aatctcgtcg gaatgatccg
     1201 gcaggcgcat gaagagttgg ctgcccaggc cggacatgca gtgaatatca gtctcactct
     1261 ccggaactgg atgattggtt gctatatcgc tgagtacgag ctgtgtggat cggatcgggc
     1321 aaactacgga ggaaatctcc tatccaatct tgcccaaagc cttacagcct ttaagattag
     1381 caactgcaat cgccgccagc tttatcgtta tctccgcttt taccgtcttt accctgagat
     1441 agtgggaacg ctgtccgcac aattgaagaa gctgttgcca ccagaagcca gggctcgcga
     1501 aaaagtgggg acagcgtccc cacaattgtg gactcccccc gataagatca tccatctgct
     1561 tgcctacagt catctggaat tactcgttga tctagaggat gacctgaagc gtgctttcta
     1621 cgaaatcgag tgcatccgtg gcaactggtc ggtccgggag ctcaagcggc agatagacag
     1681 cctctattat gagcgatccg gtttgtccaa agacaaaaag gaactgtcga agctggcgca
     1741 aaaaggcgcc aaagctgccg accccaaact ggcaatacgc gatccctaca tcttcgaatt
     1801 tcttgggctt aaaccacgtg aagtcatgag cgagtcgcat ctggaggatc aactcctcaa
     1861 taaacttcag gattttctgc tggaacttgg ccacgggttc tgtttcgagg cccgccagaa
     1921 gcggattctg atcggcgata cacacaactt tgttgacctt gtcttttatc accggatact
     1981 caagtgtcac gtattggtgg agctgaaact cgaacatttc agccatgaaa atatcggcca
     2041 gctcaacacc tatgtgagct ggtacaaaaa gaacatgatg actgagggtg acaatccccc
     2101 catcggcatc cttctgtgca cccagcgcga ccaaacgctg gtggaatatg ccctggccgg
     2161 gatggacaac ggccttttcg tttccaaata ccagcttgaa cttcctaaga aggaggagat
     2221 gcaacagttt atcgaggagc agatacggga aagggaggat aattagtcca aggtccaatg
     2281 tccaaagtcg aaggtcgaag gtcgaaggaa cggaaatggc gacgataaca aggtttgagg
     2341 atatcgaggc ttggcagaag tcacgtgaat tgacaaggga agtctatgct gcctcgaaca
     2401 cggggctgtt cgcaaaagat tttggacttc gcgatcagat ccgtcgagct acagtttcga
     2461 tcatgtcaaa tatcgcagag ggctttgagc gaggtggaga caaagaattc gtgcagttca
     2521 tagctcaagc caaaggttct tcagcagaag ttcgggctca gctctatgtc gctttagacc
     2581 agggctacat cgataagccg accttcgacc gattggcgga aatgtcgatg cacatcaacc
     2641 ggatgctggc tgggttcatg aaatacctgc gcagttcgga gttcaaaggg agcaagtata
     2701 aatgacaata gggaagaata gtccaaggtc taatgtccaa agtccaaggt ctaatgtcca
     2761 aggtccaagg tctaatgtcc aaggtctaat gtccaaggtc caagatgaca cgaatactat
     2821 gccgactttg gacagtggac gagggacttt ggactcgctg caggccgaac agtcggcttt
     2881 ggactttaga tcaaagactt tggactccaa aaactcgcct gtgacctgcc tgggcatgac
     2941 gtttccgtcc gacgacgaac ggagaaaata cttcacggaa aagctccgcg aattcttggc
     3001 tgaccccgag tttcgcaaaa tcgaaggatt tcccatcggg gcggatgagg acattctggc
     3061 gctctccgat ccgccttact acactgcttg cccgaatcca tggattgccg attttatcaa
     3121 acactatggc aaaccttacg atcccaatgt gccatacagc cgcgagcctt ttgctgcgga
     3181 tgtaagcgaa ggcaagaacg acccgatcta caacgcacat tcctatcaca ccaaggtgcc
     3241 gcataaggcc attatgcggt acatcctcca ttataccgaa ccgggggatg ttgtttttga
     3301 cggcttctgc gggacgggga tgaccggcgt tgcggcgcag atgtgcggtg acagggctgt
     3361 ggttgaatca ttgggataca aagtggagaa cgacgggaca gtatcgcagc aggaaacgga
     3421 tgaaaacggg aaaaccatct ggaagccgtt ttccagattg ggcgcccggc gggccgtatt
     3481 aaacgattta tcccctgccg ccaccttcat cgcctataac tacaacacca cggttaatgt
     3541 gcaggctttt gagcgggaag caaagcgcat cctgaaggaa gtggaagctg aatgcggctg
     3601 gatgtatgag actttacata cggatggcaa gaccaagggc aagatcaact acacggtctg
     3661 gtctgatgtt tttgtatgcc cggaatgcac ccgcgaggtc attttctggg aagcagcggt
     3721 agataagaag gccggtgcgg tgaaggatga attctcctgt ccgcattgca aggcaaatct
     3781 tacgaagcgc aaaatggatc gtgcatgggg cacacagttc gacaaggtta tcaaccagac
     3841 aatccgacaa gccaaacaag ttcctgtact catcaactac acggtcggcg gtaaaagggc
     3901 ggagaaaacg ccagataaga gcgacttggc actcatagcg aagatcgaaa acagcgacat
     3961 tccatattgg tttccgacaa cgacaattcc gaagggtgat aagacaggtg agccgcttcg
     4021 catcggcatc actcatgtgc accattttta tacaaaacgt aatttatggg tgttagcagc
     4081 tttgtggcaa cgatttacaa attaccccat gggcaagtta gcaattactt cagtactaat
     4141 caaaacagcg agccttttgc ataatattgg attcaagaac ggtaaaatta accttgcagg
     4201 agcattgcca aacgctcttt tcatcccttc aaacatagca gaaagaaata tttttgaatt
     4261 ggttcaagga aaggttgatg acttcatccg cgccaacctt catagaattc aaagcagaca
     4321 aataatatca actctttcat tgagcgcgga aaatcaaaat agcaatattt atagtagcgc
     4381 cgattacatt ttcatcgatc cgccattcgg atcaaactta ttctattcag aattgagctt
     4441 tatctgggaa gcctggctgg gtgtaatgac aaacaacaag tccgaggcca ttgaaagcca
     4501 tagccaaaac aagacacttg atgattaccg tatcttgatg actttttgtt tcaggcaagc
     4561 ataccgattc ttgaggccag gccgttggat gactgtggaa ttctcaaata caggggctgc
     4621 ggtatggaat agtatacaga cggcactttc tgatgctggt tttgttgttg gcaatgtggc
     4681 cgccctacac aagggacaag ggacttttaa ttctcaaacc aatcctactt ctgtaaagca
     4741 agatctcgtc atttccgcct ataagccaaa tggcggtttt gaagagcggt tccaaagaga
     4801 agccagcacc gaggaaggtg tctgggattt tgtccgcacg cacctcaaat atctgcctgt
     4861 cgttaaacgc ggtgcaactg ctactcatct gatcgctgtt acggaacgcg atcctcgcat
     4921 tctgtatgat caaatggtgg cctactatgt tcgtaaagga tatccagtgc cgctgaacag
     4981 tgcggaattt caggaaggac tttctcagag attctcatat cgggatggta tgtattttct
     5041 gcccgaccaa gtggccgaat acgacaagaa aaagatgacc gcaggtggta tggagcagat
     5101 gtcgcttttt gtttccgatg aatcctctgc catcgcctgg ctgcgtcaac tactcaaaga
     5161 caagcctcaa tcatttcagg acattcatcc gcattttatc aaggagcttg gcgggtggca
     5221 gaagtatgaa aagccactgg agctttctga gcttctgggt cagaacttct tttgctacga
     5281 cggtaaaggt gatatcccca gccagattca cagttatctt tcgacgaatt ttaaagagct
     5341 tcgcaacttg ccaaaggatg acgccggcct tcgagcaaaa gcaaaagatc gctggtatgt
     5401 tcctgatccg aacaaagcag gcgacctgga gaaactgcgt gagcgggcct tgctgcgtga
     5461 atttgaagat tacaggacga ccagccagaa gcgtttgaaa atatttcgcc ttgaagctgt
     5521 tcgcgccgga ttcaagaagg catggcaaga gcgcgactat gccacgatca tcaccgtggc
     5581 ccgcaaggtt ccggaaaacg tcctccagga agacccgaag ctcctcatgt ggtatgatca
     5641 ggcgctgacg agatcaggtg aagaatcata gtaatcatta aaggagcaga gatatgataa
     5701 caaaatggac gttatcaaat ttcaaatctg taagtaagga aacggagcta tcttttgctc
     5761 cacttaccct gtttgctgga ccaaacagca gcgggaaaag tacaattctt caatcactgc
     5821 tgcttgtgag ccaaaccttg gcacataagg tcagcagccg ttccgtagtg cttaacggat
     5881 taattacacg acttggtcaa tttgatgatc tcaagtctca tgaaagcgat tacgatgaga
     5941 taacaatcgg atgggagtgc cgacctctcg aaggttcgca agcgtcaacc ttggagaatt
     6001 cacgcttgcc aagtcatttt atgatttatg gaaggcataa tgatgccatt aaggcgatta
     6061 cctgtaaatt gtcattcgac gcaaatccag gcagtccgga tcgtgagcta taccaacttc
     6121 aacctcggct ttttttgttt acgatctctg ccgtggttaa ttccgatgat aatgcagatc
     6181 gacgccatga attaacggtt tcaaggtctt cggctgaatc aactaaaatt gataatctta
     6241 atgctgaagt tccaaatgat gtggcacgcg ccagtttgga atttaatgta gacattgata
     6301 atgattcctt agaggaattg cgtgaagagt ttaaaactgc tgaaccagtc ggctgtatgt
     6361 tacggcactt tctgccagat cggctttcaa tccgaattaa tgcgattaag gaagttgccc
     6421 ggattataca gctggcatta acggatgatg ggccaagaat ccggcgctca ttatttatga
     6481 aacgcgagct cgtgataccc tatcctctaa ttaaagaact tcaaaatcga cttggtgaaa
     6541 aatatgcagt cctatttgaa aaacaaacta atctctttga tgggaatgaa ggcatggccg
     6601 ttcgcgagtg gatggagcga ctccgtaagc ttccatttga tagaagacgt gaattacgcc
     6661 gcacgttagc agagtggtct gatcttgccg aaattattga gcgcgaatta cgtgatgcct
     6721 cacaaaaaga ggacaaagag tatctgctgc tgccgctgct tctccccgcc gatttgatga
     6781 atgcatcttc ttacttgggt gcaatgtttt ccaatgcggt aaaatattta ggtccgctac
     6841 gcgatgagcc aaaaccttta tatccactta cggctcatgc cgatccaacc gatgtgggat
     6901 ttcaagggga gatgactgct gcggtcttaa atttacatcg cgaacgaaaa atcagataca
     6961 tttcgtcttc agcttttgca aagacacctt ttttgcccac gccagtgaca cgctcactcg
     7021 aatcggctgt taccgattgg ttgcagtatt taggcgttgc agaaacagca tacagtattg
     7081 ataaaggtaa actgggccat gagttgaaag taaagctaac tgaaaatggg ccagtgcatg
     7141 atcttacgca tgtaggcgtt ggtgtcagtc aggtgctgcc tattgttgtt atgtcactac
     7201 ttgctgaccg tgacacgaca ctaatttttg agcaaccgga actgcacctg cacccaaagg
     7261 tacaaaccag actggctgat ttcttcttgt cgattgtatt gcttggtaaa caatgtctga
     7321 tagaaacgca tagtgagtat ctcattaatc ggcttcgtta tcgggccgcc gcagaagacg
     7381 aaaataaccg tgtgaatgat aatctcaaga tatattttgt tgagaaagag aatggttcat
     7441 ccaatttccg ggaagtaaaa gtaaatgaat acggagctat tttagaatgg ccggaaggat
     7501 tctttgacca gagccaaagg gaggctgaag atattctaag ggctgcaatg acaaaaaaga
     7561 agaaaaaaca aaaacgtgag agcaaggata atgtctgagg catttcaact ggcaatcgac
     7621 ccatatattc tttgtcttcc taatccctgc aactcattag aagaaattga gtcgtttgtt
     7681 gacacattgc ttggctggag cagcacgctg caacaaaaga attcaaatat cttaatttct
     7741 gatgctgcaa gaattgctct tttggaggat ggtgaatacc cacatcagca tagattgcgt
     7801 gatctgatgc gcatacataa atgtgaagta gctgatcctg aaactgtagg ccgccttacg
     7861 caaagtttgc ttgaccgtac accatcactt gaggcgtttt atggggttaa ctcaatactc
     7921 atcgatccgg caaaatcttc aattatgccg gatgcttttt ttaaacgcct caaggataac
     7981 actcgtaatg ctttaattga gatgctggtt atcgtatcag ctgaacagaa tgcagattgc
     8041 tcccacatag aaaaacatat attcctcgca acatcagatg atttgataga taaggataac
     8101 gatttaacag aaattgaatg cgtttcagag ttacatgact ggcagtggct gactgaacca
     8161 cttcatgatt taccaacaat gcccgcaacg attgaaggta aattaccact tgtgtttagt
     8221 cattacagct ttttgcacca acttggtatc tggcatgtat ggaataatgc gtcggatgaa
     8281 caagctgcta ttgacgcaat aaatctatgc atcgaagaaa tcgtgaacac aggcgcaacc
     8341 ggtatcagta agataaattt tcgattggga acctattttt tggagtcggt gcgtgcgcaa
     8401 agctttggaa gccgaagcga ctatgcaaga gtgctgatag aatcttgtgc acgcatcaca
     8461 ttagacatcc ccaaaaaccc tgttaaacct ttccgtgaaa gcgcaaactc aatgcgccag
     8521 aaaaggcgtc atgacggcgc tttggcattt agaacacacc tgacaaaaaa gggtgtaggt
     8581 tttcgtctta tgttgtggaa gttgtcagat ggaaccatag aatttgccaa tgttggctgc
     8641 aagggtgaac tggtaattct atgatgatag aaaatttaca tctcggcgtt tggttatggg
     8701 caattgacca cagtcagccc tgtcaggtca tcgaaaccca gacgctctgg ggcgagacca
     8761 tgtgccgtgt ttggatgcca ggtcgcgaca cggttgtccg catccccgca tcccagctca
     8821 tttctttgga gaaagcagac tctgattcac cacataatat tgcctacgcc gctgccgcgg
     8881 cgcgtgtggc cgatgcgctg acccaagaca tactccttgc gccaatagcg tcttccgtta
     8941 ttccgctccc tcaccagata agggctttgt cccgtgccat agccaatgat caggtgcgct
     9001 atcttctggc cgatgaagtt ggattaggta aaacgattga agcaggattg atttttcgtg
     9061 aacttaaact gcgtggattg gtcaaacgtg cgctggtgat tgctcccaaa gggttggtga
     9121 atcagtgggt ggccgaaatg cggctgcact tcaacgaaca tttccagctt gtcttaccgg
     9181 acgatataaa gacgctgagc cgcatttccg tagtccaaag tcaaaagtcc aatgtccaag
     9241 gtcaagatta caaaagcatt cgaccttgga cttcggacag gggactttgg acttcaggca
     9301 agggaccttg gactctttgt gatcaagttg tcgtgccgat ggattctata aaaccactgg
     9361 ataaacgccg tgggtggagc gccgctcagg ttggcgagta taatcgcgag cggtttgagg
     9421 atttgattgc cgcgggctgg gatcttgtca tcgtggatga ggctcatcgt cttggcggca
     9481 gcacggatca ggtggcccgc tttaagttgg gtcaggggct tgccgaggct gcgccttatt
     9541 tccttctgct ttccgcaacg ccacaccagg gaaagacgga cgcttttcat cgcctggttt
     9601 ctttgatcga tgcgcaagaa tttccggatg tcggcagcgt cacgcgcgcg cgcgtctcgc
     9661 cttacgtgat ccgaacggaa aaaaaacgcg caatcgacgc cgaaggaaag cctctgttta
     9721 agtcgcgccg cacggagttg tttgcggttt cctggggcaa acgccatcgg gaacagcagc
     9781 ttctttatga ggcggtaacc gattatgtgc gggaaggcta taaccaggcc atgcgggaga
     9841 agcgtagtta catcggcttt ctgatgatcc tgatgcaacg tctggtggtt tcgagtacaa
     9901 gcgccatccg tacaaccctt gaaaagcgcc tgaacattct ggcctcacca caggaacaac
     9961 tttccttgtt tcctgcggct tcggaagagg aatggacgga tcttgacggt caggagcaga
    10021 tggagattct gcttcgcacg cggttaaagg cgcttaaaaa tgagcgcgcc gaggtcaaac
    10081 tgcttttgga ggcggctgcc cgttgcgagc agatcgggcc cgatgcaaag gcagatgctc
    10141 tgctggaatg gctttatcgt ctccaatccg aagaaggtga tccggaactg aaagcgctga
    10201 tttttactga atttgttccc acacaggaaa tgctgcggcg attcctggtc gagcgcgggt
    10261 tttcggttgt ttgcctgaat gggtccatgg atatggacga gcgcaagcgt gttcaggatg
    10321 cctttgctaa ggatgttcgc attctcattt caaccgatgc cggcggcgag ggcttaaatc
    10381 tccagttttg tcatgccgtg attaattatg atatcccttg gaatccgatg cggcttgaac
    10441 aacggatcgg ccgcgtggat cgcatcggac agtcgcacgt tgtccgtgcg gtaaatttcg
    10501 tttttgaagg ctcggtcgaa caccgggtcc gcgaagtgct ggaacagaaa ctggccatca
    10561 tttttgagga attcggtatt gataaaaccg gcgatgtcct cgactccggc caggcaggca
    10621 agatgtttga cgaaatgtac atcgaagcga tccgcaatcc cgataaaatt gaggattccg
    10681 tggaaagtgt cgtaacccgc atgcaggagc aggttcgtga agcgcaaaat accgtttctg
    10741 tgctcggtaa agcggaagac ttgcagccgt ctgaagcgca acgactgctc ggccatccct
    10801 tgcctcattg ggtggaacgc atgacggtca gttatctcca ggcaaacggc ggtcgggctg
    10861 aaaggaagag ccaaggctgg aagcttacct ggcctgacgg tcaatatgat gaggatgttg
    10921 tctttaccgg caaggaggca gaacgttttc cggccgcccg gcactttact ctggaagaat
    10981 caaaaattcg cgggttggcc atgcggcttc cccggtttgt ttccgggcaa ccgattcctg
    11041 tggtttccct tcccagcata tcgcaagaga tcagcgggtt atggtcttta tggcaaattg
    11101 ccatatccac aatggggtgg aatcgtcgaa ggataatgcc attattttta gcagacagtg
    11161 gcgccgtatt gatgccgacg gccagacaca tctggaatac gcttttggtc gaaaaaatcc
    11221 agactctatc cgttgtggat tcgtccgcct ctctcacgtc ttttaatgaa ctgcaaaaag
    11281 tggcccagga gcatgggaaa gcgatctatg atgatttact tcaagagcac cggacacgaa
    11341 tgattcgcga gcgcgagaaa ggcgaatatg ccttcgccgc acgtcgcagg atgataggaa
    11401 ggattggtct gtctcaagtc cgcaatcatc gtttggccct cctgaatcag gaagagaaga
    11461 tttttaatga acagcttgat caaaaaaacc gaatctatcc cgatatgtcg ccgctgcttg
    11521 taattcgcgt ggaggggaat cattaatgaa tagctggcgc gatcatgtct taaaagaatt
    11581 taccccgaaa gtatcccgcc tgacccttgt gtccgacccg gacgggcttt tgctggaaga
    11641 aggaattctc gaaggaatcc gcaaacgggg ttttgatttg attccattcg aagatcatgt
    11701 cgcgtttcgc tttgcctatg aatcaaaata ccggtcgtgc tgggatcggg gagaacaaac
    11761 ggatctggtt gtcctgcttc gctctgaaat taaagatatt gatgggctgc catatgatct
    11821 cctccagtcg ggtcgaaaat tgtttttcaa tgttggagat gtttttccaa agctcagcca
    11881 tccggttatc gctgctttgg accgaagtta ttttgaaatc ctttatcagg cgcagaagct
    11941 acacgcgccc ggcgaattgg gagaaaacgc aacaaaggag tttattctcc gtcatgtctt
    12001 tgaaattgcc ccggagttaa tcaggcagcc atcgaatctt atgcaggttc ttttgcgtcg
    12061 tcactatcgc gggcaagtga tcccccctgt gctagacgag aggttgattc aacttcttca
    12121 gcagaataag caatttgtgg gctggcccct ggaaacaatt gttaaggatc gtgaagcttt
    12181 ctttactttc ctccaggaac gctggccgac atttcttgat aaagaagcag tccatgtgaa
    12241 aaagggcgtc tatgaagcgc atgatgaata tcatttagcc ctcaaggggc cggttgatct
    12301 gccttttgat catcatgata ttcggattta cattgataat ctcttcatgg aaggattctt
    12361 acatcctgtg cagcatgaac atgccgatga attgtccagg tcatgggtgg ctgtaggcat
    12421 tcaaacaaat gcagcagagg attgttctcg ccgtttgtgt aagcttgctg aaagtttaaa
    12481 ggcggccatt cctgctgaag atgcaaaaca ttcggattgg tcgcattttg cgcgcagttg
    12541 ggcgaaattg atgtgtcttg taaacgagga accatcatct gttccagcca caacaaaaag
    12601 taagatacgg gcacttcaga cgcagataga tattggcttt tcaacctggc ttttcaaacg
    12661 ttatgcggga ttgattaatt tgccgccggc gccccccgtt atgctgcatc acattccacg
    12721 ttttattgcg cgtcagatga gtgatgctgc tggtttaaaa gtggctttgc tggttgtcga
    12781 tggtctttct cttgatcaat ggctggttgt caaaaatatt cttggcgcaa attcatcgtc
    12841 tttcatcttt cgggagaaaa cggtttttgc ctggattcct tccataacat cgatttccag
    12901 gcaggctgcg ttttccggta aaatacctgc tttctttcca gacagcattt acacaacgga
    12961 aaaagaaagc ctcttatggt cacagttttg gacggatcaa gggctgtcac aaaacgaggt
    13021 tgtttatatc aaaggactgg gtgatggcag cctggatatg gtttctgaaa tgatttccag
    13081 ccccaaggca agagttgtcg gtctggtcgt ggacaaagtg gaccggatta tgcacggtat
    13141 ggagctgggc gctgccggaa tgcataatca agtggaccaa tgggcaaggc aattatatct
    13201 gcaaaagctt gtggatttgc ttttggatca ggggttccgt gtctatctga cgtctgacca
    13261 cggcaacatc gatgcagaag gctgtggccg accggctgag ggggtaatag cagatttgcg
    13321 aggcgagcgc gtccgtattt atcccgacat tgcccttcgc actaaaataa aagggcaatt
    13381 tccggctgca atcgaatgga ataatgttgg aatcccggat gattgcgttg ctcttctggc
    13441 gccgcatcgg caagccttta tagaaacaaa tcgtagcgca ataactcacg gcggtctttc
    13501 catggaagag attatcgtcc cctttgttca gattgaaagg aagattaaat gacgtcaaaa
    13561 agatttgagc aaattgggct tgatcgtctt gtgcatttaa cgtggctcga aaaaacggcg
    13621 aatttagctc tcgccggcaa tgatgccaaa acgattaaaa gcatcctgca agatgaatta
    13681 gcgggcgcat ttcgttcagc caataaagaa gtacgcggtt ccctggataa gaccattacg
    13741 atccttttaa aaatatggct tatgccgccg aaagattttg aaaccttgcg tcttctcggg
    13801 ctggaactga tgaaacaaac gcctcttaat gagcgtacgc ttattcattg gggaatgatc
    13861 gcagccgtgt atccattttg gtccgccgtg gccgcacaaa tcggtcgcct gttgaggctt
    13921 cagggcaccg ttacggcaat tcaaatccaa cgtcgcattc gtgaacaata tggcgaaagg
    13981 gaaacagtat cccgcagggc acgatacatt atacgatcgt ttttggactg gggagtgatt
    14041 caggaaacga atacaaaagg aatctacaaa tcggaaaaca ctcttgctgt cgatgatatt
    14101 cggttgattg cctggttggt cgaagcatcc ctttatgcac gttccaacgg agcagttcct
    14161 ttaaaagacc tgatagacag tcccgcttta ttccccttcc ggataaaatc tattcgcgcc
    14221 gaaaccctca ttgcggtatc gcccaggctt gatttcctgc gtcacggttt agatgaagat
    14281 ttgattatgc tgaagcaata acatagaagc aggcgcattt ttaatcacag gaggggaaga
    14341 tgaaaaacgt agaaatgaaa ctggaaggcg atattctgac gatcaaagtg gacgtgacca
    14401 aagagttcgg cccgtcggcc tccgggaaga ccatcatcat tgcgtcaacg gaaggcaata
    14461 tttccgttcc ggaaaaggat gacgtcaaaa tcgggctgaa tgtttatcga aagaaaccgg
    14521 tggcggcata aaacccgtca agctatgaga accttgatgg gttgcgccta tcaaacacat
    14581 cagattggca tactctacag caccgcaatc tcttccctgt cctgattccg tatgtagtcg
    14641 tccctgaaaa agtcatttat ttctaaagag catgagcggg gatcatttt
//
LOCUS       DAOSME010000055        14676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4816_length_14676_cov_20.726284, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000055 DAOSME010000000
VERSION     DAOSME010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14676
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4816_length_14676_cov_20.726284"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1323
                     /locus_tag="PLG98_08430"
     CDS             217..1323
                     /locus_tag="PLG98_08430"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HOE33322.1"
                     /translation="MRFENLKIACVIPVLFCLLTLSATVHAAINCPGTLPEGTRGVAY
                     SYTFTATNGNGGSPWWRIVMGSLPPGLSLINQSLNSVTLAGTPTTAGTYWFAINHRQP
                     GMDPICFSTIVINAAGCVLNGTDTGGISFGNLDPSGYPAPVNNIITQQVNFQCSAGLA
                     YSIAVTNPVGQLQLTGANTVPFTLGLHANGTGAGAVDIPLLTMNSAITSYAGFVEGVS
                     SFSNPITVEINWAGGSLSAYVNVSDVTVIPACGTPTNGTMNFDIDPSLNTTITASTTA
                     NGTSPTVKCTNASQPNVTCETSGGSTAGQLKKGTDNINYNVTTCGVSGKITGNGFGAA
                     PASEIPVGISVVPSAYQSAPPGDYSDNVTVTIAY"
     gene            1476..2246
                     /locus_tag="PLG98_08435"
     CDS             1476..2246
                     /locus_tag="PLG98_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HOE33323.1"
                     /translation="MNKIVGFLAVILFVIIYSTHALAADFQVQPTTMDLGGKVKSGVF
                     SVINNSNEKIDFQVSVKAWNQDENSKDAYTDTNDIVFFPKVMSIDPNSQRAVRIGLKT
                     PPGAREKTYRLFVQEIPTQKKTQDIDVNQKVKAGVTIAFRFSMPIFVKPLKPQEIYAI
                     DKVEMAAGKARAVVKNTGNVHVKLRSVTFSGKAADGKELYSKEVAGWYILNGMSSSYE
                     AEIPKDVCGQLAKIDINVRTEDKDISGILNVQKNMCPE"
     gene            2218..4923
                     /locus_tag="PLG98_08440"
     CDS             2218..4923
                     /locus_tag="PLG98_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HOE33324.1"
                     /translation="MFKRTCVPNNFTDFSTRYLSRRGSCEKTFFLVAVVFFLITFFAA
                     PAYTAEETARSARQVPAESGKSSAFTPKETTPIVVSVIINTQSRGDIFAELDDRGNLY
                     IRPDDAKALKLQYSEDRIVILRGDEPHVPLNALLDVTSTFDEKKLTVTVIGKTTESTR
                     TAADLFSLRAETKNIYYPRETSAFVNYGVNYAFTGQGGFQTLSVTNKLGFRTGDMFFT
                     SDSVFTKTESDYDLVRLQSSATYERRKDLQWLVMGDQYANSGELGSTVNMGGIGFSKV
                     YRLDPYFITQPVMDLQGTVIYPTQAEIYLNGVLIGKQDIAPGSFDLKNIYSQTGSHNI
                     EVVLKDPFGNVQRISYMAYFSAQMLRKGLHEYSYNAGFLREQYGSESNEYGDLAFSAF
                     HRYGVTNKVNIGASAEGSDGVYNGGISTTFAVPLAGQFTVQLSGSSSDEIDKRGDAVS
                     LQHNLQLGNFSTNLLLRKYSRYYATIGNPLPTEDNTRHEIRAGMGFSLNPLGSFSVNY
                     AQTENFKGMDTQVISLNYSRTLYRSISLFATGSATRATGEDTNYSGFIGLNFALMDNV
                     RGSAQVNFGSDDVNSETLQVQKDIPVGEGLGYRASVNRSETAANTVYTTNPYVQYNAR
                     YGIYSFDAQIQDSKNGISTESYNLSAAGSMVWAGEYLGLSRPVSDSFGVVLFNKNVPG
                     ASVLNNGQEIGKVGSLGTMVVPTMSSYGQNKITLDTKNIPIDFTISDVNKTLSPSLWS
                     GSCIYFDAQQLRAVTGTIFARKEGKKIPLEYVEIAMKIGEKELLSPTGKGGEFYLENS
                     FGEDDAAGSSTVADKQSCHAITRMIKAGGSTISPGTYKASVEYEGGECGFAVTFPETE
                     EVITELGEIECVATSNSPRRKDTSLRRRSLYYADYAGKALSAIPE"
     gene            4976..5794
                     /locus_tag="PLG98_08445"
     CDS             4976..5794
                     /locus_tag="PLG98_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE33325.1"
                     /translation="MFKISEQESILLFYMGRSPLGILLYPVYAFLVGDVLIDTGTNRA
                     GHEFLEALKGRRIAGIINTHHHEDHIGNNADVQQHFGINIYAHPLTLPYLENPRLNDL
                     HLYQRIVWDWPRPSKGEAVGASVKAGPYHLEVIHSPGHAEDHICLYEPDKKWLFTGDL
                     FCGTNFVYLRQDENYLQILDTLKKLSALNIETIFCNLKGVVKDGKIALAKKISTMERL
                     RDDVFRLRDRGLSPQRIRQKVLGREDARHFFTRGHYSKQNTVDSILSGKRPANR"
     gene            complement(5885..7693)
                     /locus_tag="PLG98_08450"
     CDS             complement(5885..7693)
                     /locus_tag="PLG98_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOE33326.1"
                     /translation="MHKFTLDKPDNLVDWWEESVTKFSERPLFGTKNKEGVYKWATYK
                     EIGNRIDNLRAGLTQLGIGKDDVVGIIANNRPEWAVIGYATWGCLARYVPMYEAELVQ
                     VWKYIINDSGAKVLFVSNPAIYEKIKDFPKDIPTLKHIFIIESDGDNSMASLEKKGAA
                     KPVAPKSPKAEDVAELIYTSGTTGNPKGVLLMHMNFTSNSHAGLKMYPELYENEVVSL
                     TILPWAHVFGQTAELFAIIRLGGRMGLIESTKTIINDIVQIKPTFIIAVPTVFNRIYD
                     GLWNKMNKDGGLARALFVMGVEAAKKKRILAEKGQSDLMTNFKVAVADKIVFKKIRER
                     MGGRMLGSMTGSAAMNVEISKFFFDIGIPIYDCYGLTETSPGITMNGSQAYRIGSVGR
                     PIDKVKVVIDSSVVEEGATDGEIIAYGPNVMKGYHNRPEDTKAALTPDGGFRTGDRGR
                     LDKDGYLFITGRIKEQYKLENGKFCFPVSLEENICLASFVQQAVVYGLNRPYNVCIVV
                     PDFDVLLDYAKEKGLPTDINTLVEREDIIHMISEAVTGQLKGKFGGYEIPKKFIILPE
                     AFSLDNGMLTQTMKLKRKVILDKLNDRIEALYKEDK"
     gene            8096..8305
                     /locus_tag="PLG98_08455"
     CDS             8096..8305
                     /locus_tag="PLG98_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOE33327.1"
                     /translation="MSSIYKVIEVIGGSEKSWEEAAKKAVEMTAKSLKEIRIAEVKEL
                     DMRVEDGKVVEYRVKLRVSFKYLPD"
     gene            8505..9356
                     /locus_tag="PLG98_08460"
     CDS             8505..9356
                     /locus_tag="PLG98_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE33328.1"
                     /translation="MRIEKIEDKCSGCMLCVHDCVMGVWRVVDGQSRPVAVDLCNGCS
                     HCIAICPCDAIVHDGLRKEELAGINRKNLNPDVYRDIVMSRRSIRQFRNEPVPRELIE
                     KVLDLVRYAPTARNHQDVGYIVVTDKPLIEKTAADLFGWGNRLYNMTTSGIGRRIINL
                     TGLNKNPYYNLMAYVQMQNAEKGRDYILHHAPVVILIHAAKRKMFVCDDSNIAAATII
                     NYAHTLGLGTCFVGMMTLMLRFSGRLRKTFGVPDNRRVYATLVMGYPAYRYKNTVSRK
                     KAEVQWR"
     gene            9353..10360
                     /locus_tag="PLG98_08465"
     CDS             9353..10360
                     /locus_tag="PLG98_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE33329.1"
                     /translation="MTKKIIMLVLLMAVLFHIAWYAVSRSVQHEKIPDDATAQAAFDK
                     LLKDEEISIDRHAFTEMSIEANGIRIHLDVFSKGSNGPTIVFIPGTSVYSKFYIESMY
                     KLYLQGFNVVGFDPRGHGMSSGLRGDYTVNEIVEDTLAVVKFARKRFSGPVAVVGSSQ
                     GGIAAFYAAARDDSLAAAVCHNLADLNGKDNLILSQIRPPVWMAPIAGFLADLYKRFS
                     VPVFLYLDLTQEKSRSGTDAKTLIKNNPVTVKWITLRALGSLMKTDLAKPVEQIKVPV
                     MLIHAERDSIFPQAYVEDIFNRLTCPKKYLFFKDTEHLVMTNNVDDVVPPIAAWLRDM
                     M"
     gene            10600..11040
                     /locus_tag="PLG98_08470"
     CDS             10600..11040
                     /locus_tag="PLG98_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE33330.1"
                     /translation="MTYIKINFGSNFEDEFQKVVDEVFHLVRPAFHNYECIWRPNVDV
                     YESTDEIIVLADMAGLNKEELHIEVNRKQIKIAGVRKAVQHLQNAHYCQAEIPHGYFE
                     RIISLPAPVESQSAKASYADGILMVRINKLPVSKSHRISIISEK"
     gene            11080..13503
                     /gene="lon"
                     /locus_tag="PLG98_08475"
     CDS             11080..13503
                     /gene="lon"
                     /locus_tag="PLG98_08475"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOE33331.1"
                     /translation="MTEQTVPQQNKNTFNIPEIIPILPLHNVLVFPKTMIPLEVTGSA
                     SVLVDEAMTRDRLIGLIMSRKEPDKPNDYKKDDLHPVGTCAVIMKMAKTMDNHTQLLL
                     QGVSRFSIEEIIEGKPYQQARIKLLEEKDAKDLETEALMANLLSLFDRILKLSPFLPP
                     EFGPMAKSITEAGTLADLIASIINAPVEEKQKILNIADIKKRLKELTLMVNHQMDVLE
                     LGNKIQTKVKDDIDKSQREYYLRQQLKAIRQELGETDENAVETEEYRKKIEEKNLPEE
                     AKKEALRELERLSRMHPSSAEYTVSSTYLDWMTTLPWNESTNDNLDIGKARRVLDEDH
                     YGLTKPKKRIIEYLAVRKLKPDSKGPILCFVGPPGTGKTSLGQSIARALERKFVRISL
                     GGVRDEAEIRGHRRTYIGALPGRIIQGLRRAESNNPVFMLDEIDKVGSDFRGDPSSAL
                     LEVLDPQQNNTFSDHYLDVPFDLSGVMFIATANMLDTIPPALLDRLEVIELNGYTQEE
                     KVKIAQRYLIPRQLKENGLNSSQFKLTHKALNAIITGYTREAGVRNLEREIANACRGV
                     AARIAEGAVKSGTLEEKDIPAYLGPVRVMTDLDARIAKPGIAIGLAWTPVGGDMLFIE
                     ATAMKGKKGLTLTGQLGDVMKESAAAALSFIRTNAYALNIKPEFFDDTDIHIHVPAGA
                     IPKDGPSAGVTVFAALTSLLTNRKVKKQLAMTGEITLRGAVLPVGGIKEKVLAAYRAG
                     IKTILLPEWNRKDLEDIPGNVRKSISFHFVKDMMDIIKLALEPVPQKKMKRASARKSK
                     PAVPSKTKK"
     gene            13532..14302
                     /locus_tag="PLG98_08480"
     CDS             13532..14302
                     /locus_tag="PLG98_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE33332.1"
                     /translation="MTAQYLLLDVEENVATATINRPDKGNSLSPQVLEDIEHTFTELS
                     RRQDVHVVVLTGGERYFSAGFDLTYIRTLQKAHNEAYTALFHRAYRAVMFCEQPVICA
                     VGGPAIAGGFDLTMMCDIRYASERARFGQREIVLSLNPTIDPLWRIVGLSNAMESALT
                     GRIWDAQEALRMGYISRIFPEGQLLKEVGAIAREMATYDRGCLKETKQLAHLLLNSSL
                     DQAMRQQEWLFRTYIGSEDNHQRIDDLQTKLASMRRNK"
     gene            14474..>14676
                     /locus_tag="PLG98_08485"
     CDS             14474..>14676
                     /locus_tag="PLG98_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33333.1"
                     /translation="MDSKPLYNSRIVNSYVKYIKKCYEYINIDELYDYAGIEPYEVAD
                     QNHWFSQEQINRFHEKLDELTGA"
BASE COUNT         3887 a         3387 c         3714 g         3688 t
ORIGIN      
        1 atagcaccaa taaggcattg ggcatcggca tcaaaattgc cgcagccgat gctcaagccg
       61 ctgtcgccca taatgattat tcggatacgg tgacactgac catcacttat tagtttcaaa
      121 aaatcgttca aatccctttg tgcagaatat ccgtctgaaa aatatttttg ctgcggtttg
      181 ctttttctaa tataataaga aaaaaggtat cgaaagatgc ggtttgaaaa tctgaaaatt
      241 gcgtgtgtga ttccggtatt gttttgtttg ctgactcttt ccgccactgt gcatgcagca
      301 attaactgtc cgggaactct tccggaaggg actcgcgggg ttgcttattc ctataccttt
      361 acagcaacaa acggaaacgg cgggtctccg tggtggcgga tcgtaatggg gtcgctccct
      421 cccggattga gtttaattaa tcaatccctg aattccgtca cccttgcagg aacgccgacc
      481 actgccggga cttactggtt tgcgattaac cacagacagc ccggtatgga tccaatatgc
      541 ttttccacaa tcgtgattaa cgcggccggc tgcgttttga acggaaccga tacggggggc
      601 atttcattcg gaaatcttga tccttcaggt tatccggctc ccgtcaataa tataatcacc
      661 cagcaggtta attttcaatg cagcgccggt ttggcatatt ctattgcggt tacaaatccc
      721 gtgggtcaat tacagttgac cggcgcaaat accgttccgt ttacgttggg cctgcatgct
      781 aacggtacag gcgcgggtgc cgtggatatt cctttgctga ccatgaattc ggcaattacc
      841 agctacgccg gttttgttga aggcgtctct tccttcagca atccgataac ggttgagatc
      901 aactgggccg gcggttcgct ttcggcctat gtcaatgttt cggatgtcac ggtgattccc
      961 gcctgcggta cgccgacaaa cggcacgatg aatttcgaca tcgatccgtc tcttaatacc
     1021 accattacgg caagtacaac tgccaacggc acgtcgccga ccgtcaaatg caccaatgcg
     1081 tcacaaccca atgtgacatg cgaaacgtcg ggcggctcca cggcaggcca attgaaaaaa
     1141 ggaacggata acattaatta caatgtcacg acctgcgggg tttcggggaa aataaccgga
     1201 aatggttttg gcgcggcgcc ggcatcggaa attcccgttg ggatatcggt tgtgccgtca
     1261 gcatatcaaa gtgcgccgcc cggggattat tcggacaatg tcacagttac gattgcatat
     1321 taggccaagg tctgcggctt gcagtgcagg tcaatctgtg ttatttgaga aacagcgttt
     1381 gggtgtaaaa ataaaataat cggagttata tgatgaaaaa taattaccgg gccacaagga
     1441 tatcgctcca aacttgtaaa aattttgatc cacccatgaa caaaattgtc ggattcctgg
     1501 ctgttatatt gttcgtaatc atctactcta cgcatgcatt ggccgccgac tttcaggtgc
     1561 aaccgacgac aatggattta ggtggaaaag taaaaagcgg ggttttctcg gtgattaata
     1621 acagcaacga gaaaatcgac tttcaggttt ccgttaaagc atggaatcag gacgagaaca
     1681 gtaaagatgc ttacaccgat acaaatgata ttgtattctt cccgaaggtt atgagtatcg
     1741 atccaaattc tcaaagggcg gttagaatcg gactcaaaac accgcccggc gccagagaaa
     1801 aaacatacag gctttttgtt caggaaatcc ccacccagaa aaaaacacag gatattgacg
     1861 tcaatcaaaa ggtcaaggcc ggtgtgacca tcgccttccg gttctccatg ccgattttcg
     1921 tcaaaccgct caagccgcag gaaatatatg cgatcgacaa agtcgagatg gccgccggga
     1981 aggcaagggc ggttgtcaaa aacaccggca atgttcatgt gaaactgcgt tcagtcacgt
     2041 tcagcgggaa agccgccgat gggaaagagt tgtattccaa ggaagtggcc ggctggtata
     2101 tcttaaacgg gatgtcatcc tcttacgaag cggaaattcc caaagacgta tgcggacaac
     2161 tggccaaaat cgacatcaat gtccgaacgg aagacaaaga catcagcgga atattgaatg
     2221 ttcaaaagaa catgtgtccc gaataacttt acggattttt caaccagata tttatcgcgg
     2281 cggggatcgt gcgagaagac attttttctt gttgcggtcg tgttttttct gataaccttt
     2341 tttgccgcac cggcatatac ggccgaagaa acggctcgca gtgcgcgaca ggttcctgcc
     2401 gaatccggga aatcctctgc gttcactcca aaagaaacca ccccgattgt cgtcagtgtt
     2461 attatcaata cgcagtccag gggagatatc tttgctgagc tcgatgatcg cggtaatctg
     2521 tatatcaggc cggacgatgc caaagctctg aaacttcaat attccgaaga ccggatcgtc
     2581 attctgcgcg gggatgagcc gcatgttcct ttgaacgctc ttctggatgt gacgtccaca
     2641 ttcgacgaaa agaaactcac agtaacggtg atcggcaaga ccacggaatc gaccagaacc
     2701 gctgccgatc ttttttccct gcgggctgaa accaaaaata tctattaccc gagagagacg
     2761 agcgcctttg tcaattacgg ggtgaactac gcttttaccg gtcagggcgg atttcagacg
     2821 ttgtcggtca cgaacaaact ggggttcaga accggagata tgtttttcac atcggattct
     2881 gtttttacaa aaacggaatc cgattatgat cttgtacggc ttcaaagcag tgcgacgtat
     2941 gagcgccgga aagatttgca atggctggtg atgggggacc agtacgccaa ttccggagaa
     3001 ttgggcagta cggtgaatat gggaggcatc ggcttttcca aggtgtacag actcgatccg
     3061 tattttatca cgcaacccgt catggatctt cagggcacag ttatttatcc cacacaggcg
     3121 gaaatctatc tgaacggcgt cctgatcggg aaacaggaca ttgcccccgg atcattcgat
     3181 ttaaaaaata tttattccca gacaggctct cacaacattg aagttgttct gaaagatccg
     3241 ttcggaaatg ttcaacgaat ttcctatatg gcgtatttca gcgctcagat gctgcgcaag
     3301 ggcttgcatg aatacagcta taatgcaggt ttccttcggg aacagtacgg atcagaaagc
     3361 aatgagtacg gcgatctggc cttttccgct tttcaccgct atggcgtgac caataaagtg
     3421 aatatcggtg cgagtgcgga aggatcggat ggcgtttata acggaggcat ctccacaacc
     3481 tttgccgttc cccttgccgg acagtttacc gtgcagttgt ccggaagttc ttcggatgaa
     3541 atcgacaaaa gaggcgatgc ggtctctctg cagcacaatt tgcaactggg gaatttcagt
     3601 acgaatttac tgctgcgcaa gtattcgcga tactatgcaa ccatcggcaa tccgctgccg
     3661 acggaagata atacgcgcca cgaaatcaga gcgggcatgg gcttttcttt aaatccgctg
     3721 ggaagtttct ccgttaatta cgctcagacg gaaaatttta aagggatgga tacgcaagtc
     3781 atatcattga attactcccg gacactgtat agatctatca gtctttttgc gaccggttcg
     3841 gcgacacgcg caacagggga agatacgaac tacagcggat ttatcggttt gaactttgct
     3901 ttaatggata acgttcgcgg ttcggcacag gtgaatttcg gaagcgacga tgtaaactcc
     3961 gaaacgctgc aagtgcaaaa agacattccg gttggagaag ggctgggata ccgcgcttcc
     4021 gttaatcgtt ccgaaacagc cgccaatacc gtgtatacga ccaacccgta cgttcagtat
     4081 aacgcccgct acggcattta ttcctttgat gcccagattc aggattctaa aaacggaatc
     4141 tcaacggaat cgtataattt gtcggcggcg gggtcgatgg tttgggccgg ggaatatctg
     4201 ggcctttcgc ggccggtcag cgacagtttc ggcgtggtgc ttttcaataa aaacgttccg
     4261 ggtgcgtcag tgctcaacaa cggtcaggaa atcgggaaag tgggttctct cggcacaatg
     4321 gttgtaccca cgatgtcttc ctacggtcag aataagatta ctctcgacac gaaaaatatt
     4381 ccgattgatt ttacaatatc ggatgtcaac aagaccctgt ctccttcttt gtggagcggt
     4441 tcctgtattt atttcgatgc ccagcagttg cgcgcggtta cggggacgat ctttgcgaga
     4501 aaagaaggaa agaaaattcc gttggaatat gtcgagatcg ccatgaagat cggtgaaaaa
     4561 gaattattgt ctcctaccgg gaaaggcgga gaattctatc tggagaattc ttttggcgaa
     4621 gatgatgctg ccggatcctc caccgtcgcg gataagcaaa gttgccatgc tattacacgg
     4681 atgataaaag ccggaggcag cacgatttcc cccggaacct acaaggccag tgttgaatat
     4741 gaaggtggcg aatgcgggtt tgccgtaacc ttccccgaaa cggaagaggt catcactgaa
     4801 ctgggagaaa tcgagtgcgt tgcaacatcc aattcgcccc gacgcaagga tacatcgctt
     4861 cgtcggagga gtctctatta tgcagactat gcaggcaagg ccctgtctgc aattcctgaa
     4921 tagactttac caaaacctta acaggctgta gcgtctgtct aagcggtggg gaaaattgtt
     4981 taagatcagc gaacaggaat cgatccttct tttctatatg gggcggtcgc ccctgggaat
     5041 ccttctgtat cccgtttatg cttttttagt cggcgacgtt ctgattgata ccggtaccaa
     5101 tcgtgcgggg catgaattcc ttgaagcgtt gaagggcaga aggatcgccg ggatcatcaa
     5161 cacccatcat catgaagatc acatcggcaa caatgccgat gtccagcaac acttcgggat
     5221 caatatatac gcgcatccgc tgacgctgcc gtatttggaa aatccccgtt tgaatgattt
     5281 gcatctgtat cagcggatcg tgtgggactg gcccagaccg tcaaagggcg aggctgtcgg
     5341 cgcctctgta aaggcgggac cttatcatct ggaagtcatt cattcgccgg ggcacgccga
     5401 ggatcacata tgtttatatg agccggataa aaaatggctt ttcacgggcg atcttttttg
     5461 cggaacaaat ttcgtatatt taaggcagga cgaaaattac ttgcagatac tggacacact
     5521 gaaaaaactc agtgccctga acatagaaac tattttttgc aacctcaagg gcgttgtgaa
     5581 agacggcaaa atagccctgg caaaaaagat ttcaaccatg gagcgtttgc gggatgatgt
     5641 tttcagactg cgcgacaggg gactgtcacc gcaacgcatc cggcaaaaag ttctgggccg
     5701 ggaagatgca aggcattttt ttacgcgcgg gcattattcc aaacagaaca cggttgacag
     5761 cattctttcc ggaaagaggc cggccaatcg ataaacggag agacggatca gcaaaccaaa
     5821 gagaaaggcg tgtccggaaa acacgccttt ctctttttac gggaggtctg gaaacattat
     5881 cggattattt gtcttctttg taaagcgctt caatccggtc attcaacttg tccagaataa
     5941 ctttgcgctt gagtttcatc gtttgggtca gcatgccgtt gtccagactg aacgcttccg
     6001 gaagaatgat aaatttcttg ggaatttcat aaccgccgaa tttacctttg agttggccgg
     6061 tcacggcttc actgatcata tggataatat cctctcgttc gaccagcgtg ttgatatccg
     6121 ttggtaaccc tttttctttg gcgtagtcca aaagtacgtc gaagtcggga acgacaatgc
     6181 agacattgta gggacggttc agaccgtaaa caaccgcctg ttggacaaaa gaagccaggc
     6241 aaatgttttc ttccagcgag acggggaagc agaactttcc gttttccagt ttgtattgtt
     6301 ccttgatgcg gccggtgatg aaaaggtatc cgtccttgtc cagtcgtccg cggtcgccgg
     6361 tgcggaaacc gccgtccggt gtgagagcgg ctttggtgtc ttccggccgg ttgtgatagc
     6421 ctttcatgac attcggtccg taagcgatga tctcaccgtc ggtcgcccct tcttcaacta
     6481 cggaagagtc gataacgacc ttgaccttat cgatgggtct gccgacactg ccgatccgat
     6541 aagcctgaga gccattcatg gtgatgccgg gtgatgtttc cgtcaggccg tagcaatcgt
     6601 aaatgggaat gccgatatcg aagaagaact tggatatttc aacgttcatg gccgcgctgc
     6661 cggtcatgga tccgagcatt cgaccgccca tccgctccct gattttcttg aagacaattt
     6721 tatccgcaac ggccactttg aagtttgtca tcagatcgga ctggcctttt tccgctagga
     6781 tccttttttt cttggcggct tcgacgccca tcacaaagag cgctctggcc agtcctccgt
     6841 ccttgttcat tttattccac agaccgtcgt aaattctgtt gaaaacggtg gggacggcaa
     6901 tgataaaggt aggcttgatt tgaacaatat cattgatgat ggtttttgtg ctttcaatca
     6961 atcccatcct gccgcccagg cggatgatgg cgaataattc ggcggtctgg ccgaaaacat
     7021 gcgcccacgg caaaatggtg agagaaacga cttcattttc atagagttca ggatacattt
     7081 tcaggccggc atgtgaattg ctggtgaagt tcatatgcat cagcaaaacg cctttgggat
     7141 tgccggttgt ccccgatgtg tagatcagtt cggcgacgtc ctccgctttc ggactcttcg
     7201 gtgcaaccgg ttttgcagcg cctttcttct cgagagaggc catggaattg tcaccatcgg
     7261 actcgatgat aaagatgtgt ttgagcgtcg ggatatcttt tggaaaatct tttatctttt
     7321 catatattgc aggattggaa acaaagagaa cttttgcccc gctgtcgtta atgatgtatt
     7381 tccagacctg aaccagttcg gcttcgtaca tgggcacgta acgggccaga catccccagg
     7441 tggcataccc gatcaccgcc cattccggac gattgttggc gataatgccg acgacatcat
     7501 ctttgccgat tcccagctgg gtcagtcccg ctcgaaggtt gtcaatgcgg ttgccgattt
     7561 ctttgtaggt cgcccattta taaacgcctt ctttgttttt ggtgccgaac agcggtctct
     7621 cggaaaattt ggtcacgctt tcttcccacc aatcgaccag attgtcgggc ttgtccaatg
     7681 tgaatttgtg cataaaatac tccttgttgt atggtcacgc ggacgttgag gtcgcgcgcg
     7741 gatttcttca gaaataaata ttttcgttta cggtttgtga ctctaagtca tttttatgaa
     7801 aaattcaatc gaaacatgaa tattaaatta tggattgaat attcagaact tataataatc
     7861 atttgacgtt tcttaaaaat gaattataat ttaacaaaaa ttcataatct gttatccctt
     7921 tctgtgattt cccttttgtt ttgctgccgg gttgtgtttt ccgatctgta aacgggtatg
     7981 aacgttgtgt tttttcttac tggattgccc agtgcgttat ccaggattga cggccggaaa
     8041 gccgccgtga aatgtttaaa tatttttaaa ccattcaggc gaaaggaggg caaaaatgag
     8101 cagcatttat aaagttattg aagtgatcgg gggcagtgag aaatcatggg aagaggccgc
     8161 taaaaaggcg gttgaaatga cagcgaaaag cttgaaggaa atcagaattg cggaagtcaa
     8221 ggaactggat atgagggttg aagacgggaa agtcgttgaa taccgcgtta agctgagggt
     8281 gtcgtttaaa tatctgcctg attaaaatac caaaattgat tttagaaaag ccggcctgtt
     8341 gcagtgtctg atcaatcagc cgctgcaaca ggcaaaaaca aaacgccgcc tgtccggaat
     8401 ccggcaacga atattttatt gacatgcctg ccgcttcttt atatatccaa ctcaaaacag
     8461 aaataatctc atcaaaacag cagacgtctt tgcggaatca ttgaatgcgt attgaaaaaa
     8521 tcgaggataa atgcagtgga tgcatgcttt gcgtccatga ctgcgtgatg ggcgtatggc
     8581 gtgtagtcga cggtcaatcc agaccggtcg cggttgatct ctgcaatggc tgcagtcact
     8641 gtatcgcgat atgcccctgt gacgccattg ttcacgacgg tctcagaaaa gaagaactgg
     8701 ccgggatcaa cagaaaaaac ctgaatccgg atgtctaccg cgacatcgtc atgagccgca
     8761 gaagcatccg gcaattcaga aacgaaccgg tgccacgcga attaatcgaa aaagttcttg
     8821 atctggtccg gtatgcgcct acggcgagaa atcatcagga tgtcggctac attgtcgtga
     8881 cggataagcc gctgatcgaa aagacggcgg cggatttatt cggctgggga aaccgtttgt
     8941 ataacatgac aacatcaggc atcggccggc ggataatcaa tctcaccggc ctgaacaaaa
     9001 acccttatta taatctgatg gcgtacgtac aaatgcaaaa cgccgaaaaa ggcagggatt
     9061 acattttgca tcatgcccct gtcgtgatac tgattcacgc ggcgaaaaga aaaatgttcg
     9121 tttgcgatga ttccaatatt gccgcggcaa ccatcatcaa ttacgcacat acgctcggtc
     9181 tggggacctg ttttgtgggc atgatgaccc tgatgctgcg tttcagcggc cggctgagaa
     9241 aaacattcgg cgttccggat aaccgcaggg tctatgcgac gctggtcatg ggttacccgg
     9301 cgtatcgcta taaaaataca gtgtccagaa aaaaagcgga ggttcagtgg cgatgacaaa
     9361 aaaaattatt atgttagtcc tgttgatggc cgttttgttt catatcgcct ggtatgcggt
     9421 cagtcgttcg gttcagcatg aaaaaattcc cgatgacgca accgctcagg cagcgtttga
     9481 taaactttta aaagacgaag aaatcagcat cgatcgtcat gcctttacag aaatgtccat
     9541 cgaagccaat ggcatccgga ttcatctgga tgttttttcg aaaggcagca acgggccgac
     9601 gattgttttt attcccggca cgtccgtgta ctccaaattt tatattgaaa gcatgtataa
     9661 actttatctt cagggattca atgtagtcgg ttttgacccg cgcggtcacg ggatgagtag
     9721 cggtctccgg ggagattata ccgtcaatga aattgttgag gatacgctgg cggttgtcaa
     9781 atttgcacga aaacgattca gcggccctgt cgccgtagtc ggaagcagcc agggcggtat
     9841 tgccgccttc tacgcggcag cgcgcgatga ttcccttgcg gcggccgtat gtcacaatct
     9901 cgctgatctg aacgggaagg acaatctgat tctttctcag atcaggcccc cggtatggat
     9961 ggcgcctatt gccggatttc tggcggacct gtacaaacgt ttttccgttc cggtttttct
    10021 ttatctggat ttgacgcagg aaaaatccag aagcggaacg gatgccaaaa cgctgatcaa
    10081 aaataatccg gtcaccgtga aatggattac gctcagagcg ctagggtcgt tgatgaaaac
    10141 ggatctggcc aaaccggtgg aacaaatcaa ggtgcctgtc atgctgattc atgctgaacg
    10201 ggactccatc ttcccgcagg cttacgttga ggacattttc aaccgcttga cctgtccgaa
    10261 gaaatatctg ttttttaaag acaccgagca tctggtgatg accaacaacg tggatgacgt
    10321 ggtgccgccg attgctgcat ggctccggga catgatgtaa aagggcctct ctgtgtctgt
    10381 tgattttaat tttggcatgg tttttaaagt gtccaaatcc ggatttgatt ttatagatcg
    10441 gctgtgtatg attcaaaaag gcaaacgata ttcttgccct agcggagtcg actggagtac
    10501 aaacatgtca aacggaagaa tttttgaggc ggtatttaca agtcgtcagg gcattattat
    10561 attttaacca gtatagagac agatttgtga ggcagcaaca tgacgtacat taaaatcaat
    10621 ttcggcagta attttgaaga cgagttccag aaagtggtgg atgaagtatt tcatctggtc
    10681 agaccggcct ttcacaacta tgaatgcatc tggcgtccca atgtggatgt gtatgaatcc
    10741 accgatgaaa tcattgtact ggcggacatg gccggactca ataaggaaga actgcacatc
    10801 gaggtgaacc gaaagcagat caagatagcc ggagtgcgca aggccgttca gcatttacaa
    10861 aacgcacatt actgtcaggc cgaaattccg cacggttatt ttgaaagaat catttctttg
    10921 cctgctcccg tcgaatccca gtcggcgaaa gcgtcctatg ccgacggaat tttaatggtc
    10981 agaatcaaca agctgcccgt gagcaagtcg catcgcattt ccatcatttc ggaaaaatag
    11041 cgaaaatctc ttttcggaaa atcacactgg aggttaaaaa tgacggaaca aactgtcccg
    11101 caacaaaata agaacacttt taatatcccg gaaattatcc ccattcttcc gttgcataac
    11161 gtgcttgtgt ttcccaaaac catgattccg ctggaagtga caggcagcgc ttcggtgctg
    11221 gtggatgaag cgatgactcg ggatcgcctg attggtctga ttatgtccag aaaggaaccg
    11281 gacaaaccca acgactataa gaaagatgat ttgcatccgg tggggacctg tgccgtgatc
    11341 atgaagatgg ctaaaaccat ggataatcac acacaactgc tgctgcaggg ggtcagccgt
    11401 ttttcgatag aagaaattat tgaaggaaaa ccgtatcagc aggcgcgcat taaacttctg
    11461 gaagagaaag atgccaaaga tctggagacg gaagcactga tggccaacct gctttccctg
    11521 tttgatcgca tcctgaagct ttcacccttt ctgccgccgg aattcggtcc gatggccaaa
    11581 tccattactg aagccggaac gctggctgac ttgatcgcgt cgattatcaa tgcgccggtg
    11641 gaggaaaaac aaaagatact gaatattgcc gacatcaaaa aacgtctgaa agaacttacg
    11701 ctgatggtca atcatcagat ggatgttctg gaactcggca acaagattca gacgaaggtc
    11761 aaggacgaca tcgataagag ccagcgcgaa tactatctgc gccagcagtt gaaggccatc
    11821 cggcaggaac tcggagaaac cgatgagaat gccgtggaga cggaagaata ccgtaaaaag
    11881 atcgaagaaa aaaatcttcc tgaagaggcg aaaaaggagg ccttgcggga attggagaga
    11941 ttgtcacgca tgcatccgtc gtctgcggaa tacaccgttt cctctacgta tctggactgg
    12001 atgacgacgc tgccgtggaa tgaatcgaca aacgataatc ttgacatcgg caaggctcgc
    12061 cgggtgcttg atgaagatca ttacggtctg accaaaccca aaaaacgcat catcgaatat
    12121 ctggcggtgc gcaaactcaa gccggattcc aaaggaccga ttctgtgttt cgtcggacct
    12181 ccgggaacag gcaagacatc gctcggacag tcaattgcca gagcgctgga gcgcaagttc
    12241 gtgcgcattt cgctgggcgg tgtgcgcgac gaagcggaaa tccgcggcca tcgccgcact
    12301 tatatcggtg ctctgcccgg tcgcatcatt cagggactga gacgggcgga atcgaacaat
    12361 ccggtcttca tgctggatga aatagacaag gtcggcagcg attttcgagg cgatccgtcc
    12421 tcggcgttgc tggaggtgct tgatccacag cagaacaata ctttttcgga tcattatctg
    12481 gatgtgccgt tcgatctttc cggggttatg tttattgcta cagccaatat gctggatacg
    12541 attcccccgg cgttgctgga ccggctggaa gttattgagc tgaacggtta tacgcaggaa
    12601 gaaaaagtga agatcgccca acggtatttg atcccccggc agttgaaaga aaacgggctg
    12661 aattcgtcgc aattcaaatt gactcacaaa gcactcaacg ccatcattac ggggtacacg
    12721 cgtgaagcgg gcgtgcgcaa tctggagaga gaaatcgcca acgcctgtcg cggcgtggcg
    12781 gcgcggattg ccgaaggcgc cgtgaaatcc ggcacgctcg aggaaaagga tatcccggcc
    12841 tatctggggc cggtgcgggt tatgaccgac cttgacgccc gcattgctaa accgggcatt
    12901 gcgatcggcc tggcctggac gccggtcggg ggcgatatgc tttttattga agccacggca
    12961 atgaaaggca agaaaggact gacgctgacc ggtcagcttg gcgatgtgat gaaggaatcg
    13021 gcggcggcag cgctgagctt catccggacg aatgcttatg ccctgaatat taaaccggag
    13081 ttttttgacg atacggatat tcatatccat gttccggcag gcgccattcc caaggacggt
    13141 ccgtcggccg gggtgactgt gtttgcggcc ctgacctctc ttttgaccaa tcgcaaggtg
    13201 aaaaagcaac tggccatgac cggagaaatt acactgcgcg gtgccgtact gccggtgggc
    13261 ggcatcaagg aaaaggttct ggctgcttac cgggcgggta tcaaaacgat tcttttgcct
    13321 gagtggaatc gtaaagacct tgaagatatt ccgggcaacg tacgtaaaag catatctttc
    13381 catttcgtta aagatatgat ggacattatc aagctggctt tggaaccggt cccgcagaaa
    13441 aaaatgaaaa gagcttcggc aagaaaatca aaacccgcag tgccgtcgaa gacgaaaaaa
    13501 taaaaaataa aaaattagga aggtaaaaat tatgacagcg cagtatctgt tacttgatgt
    13561 tgaggaaaat gtggcgaccg caaccatcaa caggccggat aaaggcaatt cactttcacc
    13621 gcaggttttg gaagacatag aacacacatt tacagaactg tcgcgccgcc aggatgtcca
    13681 tgtcgtcgtc ttgaccggcg gcgaacgtta tttttctgca gggtttgatc tgacctacat
    13741 ccgtaccttg cagaaagcgc ataacgaagc ctacacggcc ctgtttcacc gggcctatcg
    13801 cgccgtcatg ttttgcgaac agccggtgat ctgcgctgtc ggcgggccgg ccattgccgg
    13861 cgggttcgat ctgaccatga tgtgcgatat ccgttacgct tcagaacgtg ccagattcgg
    13921 acaacgggaa atcgttcttt ctctcaatcc caccatagat ccgctctggc gaattgtagg
    13981 cttgagcaat gcgatggaat ccgccctgac aggtcgcatt tgggacgcac aggaagcttt
    14041 acgcatgggg tacatctcac gcattttccc cgaagggcag cttctgaaag aagtcggtgc
    14101 cattgcccgt gagatggcaa cgtatgatcg cggctgcctc aaggaaacca agcaactggc
    14161 gcatctgctt ctgaattcca gtctggatca ggccatgcgt caacaggaat ggttgttccg
    14221 tacttacatc ggttccgaag ataatcatca acgcatcgat gatctgcaga ccaaactggc
    14281 gtccatgcga aggaacaaat aagacctttc ttccggttct ggttgaatta tttttcgaat
    14341 agattccttt tatgcttaat caggatataa tcaccaagcg tctgtatttc gttgacagac
    14401 gacgagcttt tttatatact cccgcgtaaa tcaagaaagt gccaatattt atttacccag
    14461 agggagtgct tgtttggatt caaaacctct ttacaacagc cgaattgtga attcctatgt
    14521 taagtatata aaaaagtgtt acgaatatat aaacatagat gagctctatg attatgctgg
    14581 aatagagcca tacgaagttg ccgatcaaaa tcactggttt tctcaggaac aaatcaatcg
    14641 tttccatgaa aaacttgacg aactaaccgg tgcaag
//
LOCUS       DAOSME010000056        14536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_4867_length_14536_cov_21.393688, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000056 DAOSME010000000
VERSION     DAOSME010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14536
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4867_length_14536_cov_21.393688"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..996)
                     /gene="speB"
                     /locus_tag="PLG98_08490"
     CDS             complement(151..996)
                     /gene="speB"
                     /locus_tag="PLG98_08490"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOE33334.1"
                     /translation="MNFGGIYPAHSLHEATFVVVPVPYDLTSTYQPGSRRGPIAIIEA
                     STNMELYDEELKKETYLAGIHTTLPVDVDARGPKNMISAVRKKISKIVALNKIPVMLG
                     GEHSISLGAVQALKEKYPRLSVLQLDAHADLRDSYQGSPYSHASVARRISELCPLVQV
                     GIRSMSKEEGDFLPTSNVKSYSADFVGERKDWCEKISKDIKGDVFVTIDLDVFDPSIM
                     ASTGTPEPGGLYWRDVLRLLKSVSRTCKIRGFDVVELAPISGDIAPDFMAAKLIYRLM
                     GYITG"
     gene            complement(1036..1809)
                     /locus_tag="PLG98_08495"
     CDS             complement(1036..1809)
                     /locus_tag="PLG98_08495"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOE33335.1"
                     /translation="MKAIFISDAHLRKAGDERYKKLLKFFDAVQAGHVMTAADPGAKS
                     DGRTLIDHLYIAGDLFDFWFCPKEKINPEFKPVIDKLNEMKNSGIGLHLCEGNHDFFM
                     RDYFADVLGLDVAEEFMEARLDHLNVLIAHGDTADSTNSNYLMLRKILRSNVFYNFQR
                     FIPASVRWSLAGLFSTASKELTTEDGNALVKKMEPFALNKFQEGFDAVILGHCHVPAI
                     NSYDVDGRKRTFVTLGDWITHYSFVYYENNNFFIHRYRG"
     gene            2034..3026
                     /locus_tag="PLG98_08500"
     CDS             2034..3026
                     /locus_tag="PLG98_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOE33336.1"
                     /translation="MTKKQNIIKRAKDVLKIEAQSILRLVDRVDSNFSQAVNLICKSK
                     GRVIITGIGKSGLIGRKIVATLTSTGTPALFMHPVEGLHGDLGIVTKEDILIAISNSG
                     ETRELMPVITSVRHIGAPIIAFTGGLSSTLARASDIVIDVSVEKEACPFNLVPTSSST
                     ATLAIGDALAIALLAEKQFNEKDFYKFHPGGNLGSRLREKVGEAMIVGKKIPKVPTGS
                     SVKKAIEEINRKNVGFVLITDKKNKLLGILTDGDVRRMISKGISFEGKSIDDVMTPDP
                     KTIDEKMSLAQAMEMMQKREITSFAVVNEKHELKGYVHLHDILGRGGSVSISLA"
     assembly_gap    3542..3641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4335..4862
                     /locus_tag="PLG98_08505"
     CDS             4335..4862
                     /locus_tag="PLG98_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOE33337.1"
                     /translation="MALYEFDGKRPRVPDDSFVHPQAALIGDIEVGHECLIAPGVSIR
                     ADFGPVIIGDRSNVQDNSVIHVFPGSKVIIEQDVIVAHGVILHDVHIKSRCVIGIGAV
                     LLFGAVCEEDVFVSVGAVVSKGMQIPAGKVVAGNPAKITRDVSDDQKLMAKEGINYYR
                     ELCRKYIATMQQVSD"
     gene            complement(5004..6134)
                     /locus_tag="PLG98_08510"
     CDS             complement(5004..6134)
                     /locus_tag="PLG98_08510"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOE33338.1"
                     /translation="MKTKIAELFGIQYPVILPGMSWISVPELVAAVSNAGGIGYLATG
                     PLSPAKTRQSIKRIRELTDKPFGVGATLLMPGAYENALVAIEEKVPVLNISLGKGGEL
                     IKKVHAYGGKVISTVTTVEHALAAQKSGADALQVTGFEAAAHGSQVTTLVLVPTVVDA
                     VKIPVVAIGGIADGRGMAAAFALGAEGVGMGTRLSITKESPVHQLAMQKQLELGAEDT
                     IYSNRFDALYCRVLKTPSAEKAVKQGRNLAKGLAASFDIAKSLDLSWMKLATSILGGS
                     KKDKSGKPKEADTAPKQKKGEISLFQKMMKGPQTGMNLAHMAQAYVAIKKATENGDLK
                     DGVYLSGQVQGIIHDIPTVAEVINRTVEEFTRIQKDMAGKAV"
     gene            6203..6922
                     /locus_tag="PLG98_08515"
     CDS             6203..6922
                     /locus_tag="PLG98_08515"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOE33339.1"
                     /translation="MAYKLDRSIYFVKNYFLLKIASQGKMLDRNYQTGLFIIIIYWRG
                     MFMKTDKKQELIAIGAELMHLKGYNDTGVQEILGKAGLPKGSFYNYFKSKEDFGLLVV
                     DYYMAFYNKIFAKFMDNKSVPPLERIRRLYKSFNKSFRANHYTLGCPIGNFAQELGDT
                     SPVFQARLKKSADTIVRYFSDVLREAQASGHLSRSLDADKTAHYIFNSWEGAIMSMKV
                     EKSLRPLEINEQFLFDYILKP"
     gene            complement(6956..7759)
                     /locus_tag="PLG98_08520"
     CDS             complement(6956..7759)
                     /locus_tag="PLG98_08520"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOE33340.1"
                     /translation="METKLFFYTGTGNSFWTAKKLSLLLHDAETVSMALNLSGRIIAD
                     AEKIGFVFPVHIWGIPAPVIDFLNRLDMDPEKYYFAVAVNAGQVAATLIQLEKLLQQK
                     HVKLSSGFSVCLPSNYIPWGGAEAPAKQKKKFSATLDKINCIASSVRAKEEKPPERGP
                     FWQNILFSAIYRMTFERVPRMDKPFYADAKCTSCGICEKICPAHNIDLVDGKPAWQHR
                     CEQCFACLQWCPEEAIQYGKGTVKRKRYRHPEITLKEMLACVPGKNRLS"
     gene            complement(7769..8233)
                     /locus_tag="PLG98_08525"
     CDS             complement(7769..8233)
                     /locus_tag="PLG98_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOE33341.1"
                     /translation="MKKYVFLSVLFSVFLCQTATADFYKWVDEKGEIQITDYPPPENK
                     AVKNIEIHEVGSEDPLNAEGEDDASKAKNQKKPEIVLYTKNNCPDCEKAKEFLNSKNV
                     PFIEYNMDTDEKAVAKRKEVDQGDEVPFAIINRNQVYGFSESVYNKVLQMQP"
     gene            8486..9628
                     /locus_tag="PLG98_08530"
     CDS             8486..9628
                     /locus_tag="PLG98_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOE33342.1"
                     /translation="MFGKPVSLFSVFGFEIKVDMSWLILAALITWSLASGLFPEYYKD
                     LPKSAYWWMGIAGAIGLFLSIIVHELTHSIVARHYGVSMKEITLFIFGGVAHMEEDPS
                     SPKAEFMIAVVGPVSSFLLAASAFLLYKVGVYSGWPVTLTGVLNYLSWLNMILAVFNL
                     IPAFPLDGGRILRSALWKWKKDIRWSTNIAAQIGSGFGLMLIIMGIFYIVKGNFVGGL
                     WWFLIGLFVRAAAQSSYRQILARNLFHAKKVRDLMVTNPVTVPRAISLEDFIRDYVYK
                     YHFQAYPVVSFGRLTGCIFLKQVASIPREQWAQHTVGSVAVPCSKEITIGPDEDANKA
                     LDLMNRTGNSRLLVVQGDELEGILSLKDMLALLSLKMELNDMEKKT"
     gene            9953..10591
                     /locus_tag="PLG98_08535"
     CDS             9953..10591
                     /locus_tag="PLG98_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE33343.1"
                     /translation="MANDTLLEPRKIPSQERSRKTVAAIYEAATDIFVNTGYAEATTD
                     QIAEKAGVSIGTLYNYFPGKEAILYGLWEKYEKEIKVITDQADQDIRKKGSFDRSIVP
                     VLLNLALELVSYERVQNRLFISQIGLPETIIQKRRELGLYMEATMEAVFRDFSNVRVR
                     NPKIGVHIIWATVQAVFHDYIISVSDEIKPEDFIDELGDMMSRYIFADEAQE"
     gene            10854..11267
                     /locus_tag="PLG98_08540"
     CDS             10854..11267
                     /locus_tag="PLG98_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33344.1"
                     /translation="MFGTLITTHGNLGDELIKSAELIKGRLENIMHICIDQTKDVEDL
                     KKEISNAIKKLDKGNGVLILTDLFGGTPSNISLSFMKEGKVEVLTGVNLPMMLKLSEA
                     KGNMTLRDFACLVKSYGEKNIMLASEILSKKANRS"
     gene            11343..11852
                     /locus_tag="PLG98_08545"
     CDS             11343..11852
                     /locus_tag="PLG98_08545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOE33345.1"
                     /translation="MAKDLNIALVRVDSRLVHGQILEAWVPFIKAKCIIVVNDKVAAD
                     PFLETVIKMAVPSDIEVIISSLDDFVRNYSFAHGSGKKTIVLFGSIADACHAFEHGFR
                     FDRLNIGNVYNNEYKVCCSSSVFLCDQEIIRLEDLLKETGVFVELQRVPKGRAVNIKE
                     ALKDYWRVK"
     gene            11983..12678
                     /locus_tag="PLG98_08550"
     CDS             11983..12678
                     /locus_tag="PLG98_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOE33346.1"
                     /translation="MFQEIILTAFCGSLLCLDRVFMQAMISRPVVIAPLMGLLLGNLY
                     AGLIIGAIIELIWIDRLPIGTYIPPNDSLTAVLAVAIALISGSATGGVTPQIIALAVI
                     LAIPFGSLAKQMDVMIIQSNNVLSDQALRDAQENNLRAIERKNYLGLLKVFFFYGVFL
                     LAAQAVVIPLVIWGYPRLNEPLLKALSFTYYFLPLLGIAVALNMLKLRNAVPVICAVF
                     LVVSMLLEFFHVL"
     gene            12668..13156
                     /gene="rimI"
                     /locus_tag="PLG98_08555"
     CDS             12668..13156
                     /gene="rimI"
                     /locus_tag="PLG98_08555"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOE33347.1"
                     /translation="MSCKEISLPLTIDPMKKSDLPEIMKIERESFLSPWTVGMFKREI
                     SSDHSHCLCARIHSDGKEIVAGYIVFWLIIDEAHLHSLAVKKEFRGKGIAFRLMQSMK
                     TIAGKNDVLFQTLEVRASNVEAIKLYRKCGFVVKGVRPLYYSDTHEDALIMWADINPV
                     SE"
     gene            13170..13988
                     /locus_tag="PLG98_08560"
     CDS             13170..13988
                     /locus_tag="PLG98_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOE33348.1"
                     /translation="MAGNIYIGEIIANEEIQEDCFLMKVRTHSSFENPLPGQFVMVRI
                     AGLNDPFLSRPISIYSFTRETNYCTIELLYRVVGKGTQVMAGLIEGSYLEINGPLGNG
                     FETENVKENIVLISGGIGIAPLSMLMESLRRTARRSSAAIMMYTGFQNKTAVVGLDKM
                     RGACRDVSVCTDDGTLGSKGFVTQIFEKDMKNFTPENTSIYVCGPREMLKALAKILNK
                     SKFDCQVSLEERMACGTGACMGCAVAVKDKEGAFAYKRVCADGPVFNLSDIIWD"
     gene            13994..>14536
                     /locus_tag="PLG98_08565"
     CDS             13994..>14536
                     /locus_tag="PLG98_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOE33349.1"
                     /translation="MTKTKTTQSPKMAVSLGRLKLNNPVMTASGTFGYGEEYAGYVDL
                     NKLGAIVVKGLSLKPRLGNPPPRIMETTGGMLNAVGLQNIGVDAFIEEKIPFLRQYDT
                     KVIANIYGETYDEYKKVARKLSSVKGIHALEVNISCPNVKNGGLSFGSDPKIAARMTR
                     AVKDETSLPVIVKLTPNVTDI"
BASE COUNT         3594 a         3360 c         3494 g         3988 t
ORIGIN      
        1 acctctgacc tttgggttat gagcccaacg agctaccgga ctgctccacc ccgcgatatt
       61 tgaaaaacgt tgactttaca ccattatctc cactgtttgt caaggagtaa ttttcaaaat
      121 ccttgatcga tatcaacgga ttgaataatc ttacccggtt atgtagccca taaggcggta
      181 aatcagtttg gcggccatga aatcaggcgc gatatcgccg gaaatcgggg ccagttccac
      241 gacatcgaag ccgcggatct tgcaggtgcg tgaaacggat ttaagcaggc gcagcacatc
      301 cctccagtac agacctcccg gttccggtgt cccggtggac gccatgatgg aagggtcgaa
      361 aacatccaga tcgatggtga caaaaacatc gcccttgatg tccttgctga ttttctcaca
      421 ccagtctttt ctttccccga caaagtccgc gctgtaggat ttcacgttgc tcgtcggcaa
      481 aaaatcgcct tcctccttac tcatgctccg gatgcccacc tgaacaagcg ggcaaagctc
      541 agaaattctt cgggcgacgg aagcatgact gtaggggctt ccctggtagc tgtcccttaa
      601 atcggcatga gcgtccagtt gtagaacgga aagtctggga tatttttctt tcagcgcctg
      661 caccgctccc aggctgatgc tgtgttcgcc gcccagcatg acgggtattt tattcagagc
      721 gacaattttg gaaatctttt tccgcacggc tgagatcatg tttttaggcc ctcgggcgtc
      781 aacatcgacc ggaagcgtcg tgtgaattcc ggccagatac gtttctttct tcaattcctc
      841 atcgtagagc tccatgttgg tggacgcctc gatgatggct atcggtccgc ggcggcttcc
      901 cggctgatat gtcgacgtca gatcataggg aaccggcacc acgacaaatg ttgcttcatg
      961 cagtgaatgc gcgggataaa ttcctccaaa attcattatg gtcatccttt cttaaattaa
     1021 attcggtttg ggtcatcagc cccggtaacg atgaataaaa aaattattat tctcataata
     1081 gacaaaggaa taatgcgtta tccagtcgcc cagagttaca aacgttcttt tccggccgtc
     1141 cacatcataa ctattgatgg ccggcacatg acagtggcct agaataacgg catcaaaacc
     1201 ttcctgaaat ttgttcaggg cgaacggctc cattttttta actagagcgt tgccgtcttc
     1261 cgtggtgagc tccttgctgg ccgtagaaaa cagccccgcc agggaccagc gaacggacgc
     1321 cgggataaaa cgctgaaagt tgtaaaaaac gttactgcgc agaattttac gcaacatcag
     1381 gtaattggaa ttggtgctgt ctgcagtgtc gccgtgggcg atcaacacgt tcagatgatc
     1441 cagccgggct tccatgaatt cctcggcgac atccaatccc agcacatcgg cgaaatagtc
     1501 tctcataaaa aaatcgtgat tgccttcgca taagtgaagg ccgatgccgg aatttttcat
     1561 ctcgtttaat ttgtcaatca cgggtttgaa ctccggattg attttctctt tcggacaaaa
     1621 ccagaaatca aacaagtctc cggcaatgta aagatgatcg atcaacgttc tcccgtcgga
     1681 ttttgcgccc ggatcggctg ccgtcatcac atggcctgcc tgaacggcat caaaaaattt
     1741 tagcagtttc ttatatcgtt catcgccggc ttttcgtaaa tgcgcatcgg aaatgaaaat
     1801 ggctttcata ggttaaatcc gcgtgtttaa agtgaatctt gtttagcaga tacaggtctt
     1861 caggtcaata aacataaaaa agaacgcaat atcggcacat ttccggcggc ggacgaatga
     1921 tttttcccga acccggctga attggacctt gttttgtcgg gatttttttg ctagtgaaag
     1981 ctcgtcatga ccgaaacgtg atcagaattc aatgtttgca gagaaggcga aaaatgacaa
     2041 aaaaacaaaa catcatcaaa cgggcaaaag acgttctgaa aattgaagcg cagagcattt
     2101 tgcgattagt ggatagagtg gatagtaact tttctcaagc cgttaacttg atatgcaaat
     2161 ccaagggaag ggtgatcatc actggcatcg gcaagtccgg tctgatcgga cgtaaaattg
     2221 tagcaacgct caccagcacg ggcacaccgg ctctctttat gcatccggtt gagggcctgc
     2281 acggcgatct gggcatcgtt accaaagaag atatcctcat tgccatctcc aacagcggcg
     2341 aaaccagaga actgatgccc gtcatcactt ccgttcgcca tatcggcgct cccattatcg
     2401 cttttaccgg cggcctgtct tcaacgctgg ctcgggcaag cgacatcgtt attgatgtgt
     2461 ccgtggaaaa ggaggcctgt ccgttcaatc ttgttcccac gtccagttcc accgccaccc
     2521 tggcgattgg cgatgccctg gcgattgcac tgctcgcgga aaagcagttc aatgaaaaag
     2581 atttctataa atttcatccc ggaggaaatc ttggttcgag attgcgcgaa aaggttggcg
     2641 aagccatgat cgtgggaaag aaaattccca aggttccgac aggaagctcc gtgaagaagg
     2701 cgatagagga aatcaaccgc aaaaatgtcg gatttgtttt gattaccgac aagaaaaata
     2761 aattgctggg aattctcacc gacggcgatg tgcgccgtat gatcagcaaa ggcatatcgt
     2821 ttgaaggaaa atccatcgac gacgtcatga cccccgaccc gaaaaccata gatgaaaaaa
     2881 tgtctctggc tcaggccatg gaaatgatgc agaagcggga aattacctcc tttgccgtgg
     2941 tcaatgaaaa acacgaactc aagggatacg tccacttgca tgacatcctc ggccgcggcg
     3001 gctccgtcag catctctctt gcgtgatttt gcgcgtgctc aggaaatact gatccttcaa
     3061 gacctgacac aatatgtgat aaaaatggtt agtctgcaag agccaagcaa agatttgacc
     3121 tgttccccca gttgtcatcc ccgaatgggc ttatcgggga tccagtattg ttcataccat
     3181 taattagacc gtccccatta ttcccatcgc ttatccttgg gttaccaaac ctgtttccat
     3241 tattttacaa ttttaagggc gtcccgcttt cccagtatat tttcagacac aaatataaac
     3301 aactttagat cagcgaagac aaaccggaat atgagatccc atcatgttct ggtattatac
     3361 agcacacttt ttgaccaata ggatcttgca ctttttctcc caacttgaat tgtctattga
     3421 aatacaacgc agactttttg attccccatc cacgttaaag gtgtttatta tcctacgtcg
     3481 ctacgctccc aggataattc gactaactct tcaaacttca tcccgatttg tcgggattcg
     3541 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcccaggat aattcgacta
     3661 actcttcaaa cttcatcccg atttgtcggg attcgtttta ccacggcact ttgtgcctgg
     3721 tatagttcgc cttacgcttc atacttcatt ccgactcgtc gggattcgtc ttcagcttgg
     3781 ctcactatca gagagtctca ttaaaactgt ttttgtgaaa acttgtgaag cccccgtacg
     3841 caacgcttcg gggatatccc taccacagag gcggttggga ttgttcgtcc cacaaatttc
     3901 agtacgttcc acttctgaaa cttgggactc actaattcga ctaactcttc aaatcccacg
     3961 gttctttgag cctgggatag ttcgacttgc aaatattact gcactacgat tgtaatattt
     4021 gagtctcact actttgtccc gatacatcgg gactcgtttt accacggcac tttgtgcctg
     4081 gtatagttcg ccttacgctt catacttcat tccgactcgt cgggattcgt cttcagcttg
     4141 gctcactatc agagggtctc attatatttt tgattcgata agactgatat tctataaatg
     4201 aattgcccgt acttgtcaat aatgttttct taataatttc aagaggtaag aatatcatcc
     4261 tgcttacggt taactaaata tgtggcacca tgcttgtatt ttttgtatat taaaaaaaca
     4321 ggagaaataa ttgtatggcg ctttatgaat ttgacggtaa aagaccgcgg gtcccggatg
     4381 attcctttgt tcacccgcag gctgccttaa ttggagatat cgaagtcggc catgaatgtc
     4441 tgattgcgcc cggtgtttcc atccgcgccg atttcggtcc ggtcatcatc ggagatcgtt
     4501 ccaatgttca ggacaacagc gtcattcatg tctttcccgg atcaaaggtc attattgagc
     4561 aggatgtcat tgtggctcat ggcgtgattc ttcatgatgt gcacatcaaa tcccgatgtg
     4621 taatcggcat tggcgctgtt ttgcttttcg gcgcggtctg cgaagaagat gtttttgttt
     4681 ccgtgggtgc ggtcgtgtcc aaaggcatgc agattcccgc cggcaaagtg gttgcgggca
     4741 atccggcgaa aatcacccgc gatgtttctg atgatcagaa gttgatggcg aaagagggga
     4801 ttaactatta tcgggagctt tgcagaaaat acattgcaac gatgcaacaa gtcagtgatt
     4861 aaccgtccaa caatagtgac gacattggga agatagccat cacacttgtc acaccggcgg
     4921 aggccggggt ccagtttaaa taattctgga ttccggctca gaggccggaa tgaccaaatt
     4981 atagatatct acaagataat gttctaaacg gctttccccg ccatatcttt ttgaattctg
     5041 gtaaattctt caaccgttcg attgatgact tcggccacgg tcggaatgtc gtggataatg
     5101 ccctgtacct gaccggaaag atacacgccg tctttgagat cgccgttttc cgtcgctttc
     5161 ttgatggcga cataggcctg cgccatatgt gccagattca ttccggtttg cggacctttc
     5221 atcatcttct ggaataacga tatttccccc tttttttgtt tcggtgcggt atcggcttct
     5281 ttgggttttc ccgatttatc ttttttcgag ccgcctaaaa tcgatgtcgc cagcttcatc
     5341 caggataaat ccagcgattt ggcgatgtcg aatgatgcgg caagtccctt ggccagatta
     5401 cgcccctgct tgacggcttt ttcggcggag ggagttttca gaacccggca gtaaagcgcg
     5461 tcgaaccggt tggagtagat ggtgtcttcc gctcccagtt cgagttgctt ctgcatggca
     5521 agttgatgaa cgggactttc ttttgtgatc gagagcctag ttcccatacc cacgccttcg
     5581 gcgccgagcg cgaaagcggc ggccatgcct ctgccgtccg cgatgccgcc aatagcgacc
     5641 acgggaattt taaccgcatc aacaaccgtc ggaaccagaa ccagcgtggt cacctggctg
     5701 ccgtgcgccg cggcttcaaa gcccgtcacc tgaagagcgt ccgctccgga tttctgggcg
     5761 gcaagcgcgt gttcgaccgt ggtgaccgtc gatatgactt tcccgccgta ggcgtgtact
     5821 tttttgatta attctccgcc cttgcccaaa gatatgttga gaaccgggac tttttcctcg
     5881 atggcgacca gcgcgttttc atatgcgccg ggcatcagaa gcgtcgcgcc cacaccgaag
     5941 ggcttgtccg tcaattctct gattcgttta atggactgcc gtgtttttgc cgggcttaaa
     6001 ggtcccgtgg cgagatagcc gatgcctccg gcattggaga ccgcggcaac cagttccggc
     6061 acgctgatcc agctcatgcc gggaaggata accggatatt ggatcccaaa caattcggcg
     6121 atttttgttt tcataaattg tccttttaaa ataataattt ttaattataa aaaatcgaag
     6181 caagactaca atgcttcttt tcatggcgta caaattagac cggtctattt attttgtcaa
     6241 gaattatttt cttctcaaaa tagcgtcgca ggggaaaatg cttgacagaa attatcagac
     6301 cggtctattt atcatcatca tttattggcg gggaatgttt atgaagacgg ataaaaaaca
     6361 ggagctgatt gccattggcg cggagctcat gcatctgaag ggctacaatg acacgggcgt
     6421 tcaggagatt ctcggtaagg ccgggcttcc caaaggttct ttctataatt attttaagag
     6481 caaagaggac ttcggactgc tggttgttga ttactatatg gcattctata ataaaatctt
     6541 tgccaagttt atggacaata aatccgttcc gcctctggaa agaatccggc gcctttataa
     6601 atcgttcaat aaatcattcc gggctaatca ttatacgttg ggatgtccga tcggcaactt
     6661 cgctcaggag cttggagaca caagccctgt gtttcaggcc aggcttaaaa aatccgctga
     6721 taccatcgtc aggtattttt ccgatgtgct gcgcgaggct caggcatcgg ggcatctctc
     6781 acggtctctt gatgcggata aaaccgcgca ctatatcttt aacagctggg aaggcgccat
     6841 tatgagcatg aaagtggaga aaagcctccg accgctggaa atcaacgagc agtttctttt
     6901 tgattacatt ctcaaaccgt gagatactaa aactgtatat cggaaaagtc gtcgattaag
     6961 ataatctgtt tttccccggg acacaggcca gcatctcctt cagagtgatt tccggatgac
     7021 ggtatcgttt tcttttgacg gtacccttgc cgtattgaat cgcttcttcc gggcaccatt
     7081 gcagacaggc aaagcactgt tcgcaacgat gctgccatgc gggtttcccg tcaacaagat
     7141 ctatattgtg agccggacaa attttctcac agattccgca actcgtgcat tttgcatcgg
     7201 catagaaggg tttgtccatt cggggaacgc gttcaaacgt catgcggtag atggcggaaa
     7261 aaagaatatt ctgccagaag gggcctctct ccggcggttt ttcttctttg gcccggaccg
     7321 atgaggcgat gcagttgatt ttatccagcg tggcgctgaa tttcttcttc tgctttgccg
     7381 gagcttcagc gcctccccag ggaatgtagt tgctgggcag gcaaaccgag aatcccgaag
     7441 acagtttcac atgcttctgt tgcagcaatt tttccaactg gatcagcgtt gcggcaacct
     7501 ggccggcgtt gaccgccacg gcaaaataat atttttccgg atccatgtcc agacgattta
     7561 gaaaatcaat caccggtgca ggaattcccc agatatgaac cggaaaaacg aaaccgattt
     7621 tttcagcatc ggcaattatc ctgccactga ggttaagtgc catggaaacg gtttcagcgt
     7681 catggagcag cagagaaagt ttttttgccg tccagaaaga atttcccgtt ccggtataga
     7741 aaaatagttt tgtttccatt gagccacatc aaggctgcat ttgcaggacc ttgttataaa
     7801 cgctttccga gaatccgtat acctgattgc ggttgatgat cgcgaagggc acttcgtcgc
     7861 cctggtccac ttcttttctt ttcgcaacgg ccttctcatc ggtatccatg ttgtattcaa
     7921 tgaagggaac gtttttggaa ttcaagaatt ctttggcttt ttcgcagtcc ggacaattgt
     7981 ttttggtata aagcacaatt tccggtttct tctggttttt ggccttcgat gcatcatctt
     8041 cgccttcagc gttcagtgga tcttcggagc cgacctcatg aatttcaatg ttttttacag
     8101 ccttattttc cggcggtgga taatcggtaa tttgtatttc tcctttttca tcaacccact
     8161 tataaaaatc agccgttgcc gtctgacaca gaaaaacgga gaataaaacc gataggaaga
     8221 catacttttt cataacctga cctcacgata agctgtttta agaaatagtg attcttttcc
     8281 ggccggctta atcaggcttt catcaaacgt cacgatcaaa ccggttacaa tataattaaa
     8341 agtatcttaa atcatccggc gttgtcaaat tctttatcgt tgcgtcttgc ggggctggag
     8401 gagagcattc cggatgcttc tgttaaaaat attgattttc atctttcttc tatattatat
     8461 gaaacacatt ctataaggag catttatgtt tggaaaaccg gtaagccttt tttcagtgtt
     8521 tgggtttgaa ataaaagtcg atatgagctg gctgattttg gcggcgttga tcacctggtc
     8581 gctggccagc ggtcttttcc cggagtatta caaagacctt cctaaatccg cctattggtg
     8641 gatgggcatt gccggcgcca taggcctttt tttatccatt atcgtgcatg aacttaccca
     8701 ctccattgtt gcccgtcatt acggtgtatc gatgaaggaa ataacccttt ttattttcgg
     8761 cggtgttgcg cacatggaag aagatccgtc cagtcccaaa gctgaattca tgattgccgt
     8821 ggtcgggcct gtgtccagct ttctgctggc cgcctctgct tttttgcttt acaaggtcgg
     8881 ggtctacagc gggtggccgg tgactctgac gggggttctg aattatctgt cctggctgaa
     8941 catgattctg gcagtattca atctgattcc tgcttttcct ttggacggcg gacgaatcct
     9001 gcgttccgcg ctatggaaat ggaagaaaga catccgctgg tcgacgaata ttgccgctca
     9061 gatcggttca ggcttcggct tgatgctgat catcatgggc attttttata tcgttaaggg
     9121 aaatttcgtc ggggggttgt ggtggtttct gatcggtttg tttgtccgcg cggcggcgca
     9181 aagttcctac cggcagattc tcgcgcggaa tcttttccat gcgaagaaag tcagggattt
     9241 gatggtcacc aatccggtca ccgtaccgcg ggccatatcc ctggaggatt ttatccgcga
     9301 ttatgtttac aaatatcatt tccaggcgta tcccgtcgtt tcgttcggga gattgaccgg
     9361 ctgtatcttt ttaaaacagg tggcctctat cccgcgtgaa caatgggctc agcataccgt
     9421 ggggtctgtg gctgttcctt gcagcaagga gatcacaatc ggtcccgatg aggatgcgaa
     9481 taaagccctt gacctgatga atcgtaccgg caacagccgt ttgctggttg ttcagggaga
     9541 tgaacttgag gggatccttt cgctgaagga tatgcttgcg ctgctgtctt tgaaaatgga
     9601 attgaatgac atggaaaaaa agacatgagc ccttttttgt ccgaatgcag caaccacaga
     9661 tcagacgatc ggcgatcatt ttatcacata atattttttt tcgatgaaga ttcattacga
     9721 tcgtgaccgt gcgcgctccc tcttcaacat ggatctgtgg tcgacgaaga tttcttgcac
     9781 agaatggttt cacggtgcat gacccgtcag tgggaattga gcggcgcttt tggcaggtcc
     9841 tgaaggattc tttgaaaatg gcgatgatct tttcagcttg actgttcaag taagtttttt
     9901 tataagttct tacagcttca tcgtcttaca aagcttcccg gaaggactgt ttatggccaa
     9961 cgatacattg cttgaaccgc gcaaaattcc atctcaggaa cgctcgcgta aaacggtagc
    10021 ggccatctat gaagctgcga cggatatttt tgtcaatacg gggtacgcgg aagcgaccac
    10081 ggatcaaata gctgaaaaag cgggcgtttc gataggtact ttgtataact atttccccgg
    10141 caaggaggct atattatacg gtctttggga aaagtacgag aaggagatca aagtcataac
    10201 agaccaggcc gatcaggata tccgaaaaaa aggatctttt gatcgcagca ttgttccggt
    10261 tctgcttaat cttgctctgg aactggtcag ttatgaacgc gtacagaatc gactgttcat
    10321 cagccagatc ggtttgcccg aaaccattat tcaaaaacgc cgggagctcg gtctttacat
    10381 ggaagcgacc atggaagcgg tgttccgtga tttttccaat gtccgtgtcc gaaatccgaa
    10441 aatcggggta catattatct gggcgacggt gcaggctgtc tttcacgatt atatcatctc
    10501 cgtttccgat gaaatcaaac ctgaagactt tattgatgaa ctgggcgaca tgatgagccg
    10561 ctatattttt gccgacgaag cacaggaata attgcggcgg atttcgggat tcgatcggct
    10621 ggatcttaac ggtattattt cacaacctcc tgaagatcga gcagttcccc aggccctctt
    10681 gaatgataaa ttctttcttt ccggtgcggt gaatttccga ccgatacagc actaaaaaat
    10741 tatttccata aaccttgaca attatggtgc aatccattag cgtaacgctt taaattcgaa
    10801 ggcaaagacg cggtcatgat cattgcgtaa acgggttgaa aagggggtta ataatgtttg
    10861 gcacacttat taccacgcat ggcaatttgg gagatgaatt gatcaaatcg gctgaactga
    10921 tcaaaggtcg gcttgaaaat ataatgcata tctgcattga ccagacgaaa gacgtcgaag
    10981 atttgaaaaa agaaataagc aatgccatca aaaagctcga taaaggcaat ggcgtattga
    11041 ttctgaccga tcttttcggg gggaccccgt ccaatatttc gctttcgttc atgaaagaag
    11101 gtaaagtaga ggttctcacc ggcgtcaacc tgcccatgat gcttaagttg tctgaagcga
    11161 aggggaatat gacgctgcgc gattttgcct gcctggtgaa gagttacggc gaaaaaaaca
    11221 ttatgctggc cagtgaaatt ttaagcaaaa aagccaacag gtcatgaaat ccacgccctt
    11281 ttacccgcag atggccgcgc tattgaaaag ggagtcttag gattccgcga cagggggaaa
    11341 acttggcaaa agatctgaat atagctttgg ttcgtgttga tagccggttg gttcacggtc
    11401 agattttaga agcctgggtg cccttcatta aggcaaaatg catcatcgtc gttaacgata
    11461 aagtggccgc ggatcctttt cttgaaacag tcatcaaaat ggcggtgccc agcgatatcg
    11521 aagtcatcat tagttccctc gatgattttg tccggaatta ttcttttgct cacggcagcg
    11581 gaaagaaaac gatcgttttg ttcggcagca tagccgatgc gtgtcatgct tttgaacatg
    11641 gtttccgttt cgaccgattg aatatcggca acgtttataa taacgaatac aaggtatgct
    11701 gctcctcttc ggttttcctg tgcgatcagg aaattattcg tctggaggat ttgctgaaag
    11761 aaacaggagt ttttgtcgaa ttgcagagag tgccgaaagg aagagcggtc aatattaaag
    11821 aagcgctgaa ggattattgg cgtgtaaagt aagcagcgca tttgctcata tgatatgctg
    11881 ctttaaattc tttgtgtctt ttttcgcgta gagggttttt gtgttggttg cggctgtacc
    11941 ggtccgcaat gcgtttctgt tcgaaaatct ggggcgagtg acttgtttca agagattatt
    12001 ttaacggcat tttgcggcag cctgctgtgt ctggatcgcg tttttatgca ggccatgatt
    12061 tcccgcccgg tggtgattgc cccgctcatg gggctattgc tgggaaatct gtacgcggga
    12121 ctgatcatcg gagcaatcat tgaactgatc tggatcgatc gtctgccgat cggaacgtat
    12181 attcccccga atgattccct cacggcggtg ctggccgtgg ctatcgcttt aatctccggt
    12241 tcggcaacag ggggtgttac cccgcaaatc atcgcgcttg ccgtcattct ggccatcccc
    12301 tttggcagtt tggccaaaca aatggacgtc atgattatcc agtccaacaa cgtcctttct
    12361 gatcaggccc tccgggacgc gcaggaaaac aaccttcgcg ccattgaacg aaaaaattat
    12421 ctggggcttt tgaaggtatt tttcttttac ggtgtttttc tgcttgccgc tcaagcggtt
    12481 gtgatcccgc tcgtcatctg gggatatcct cgcttgaatg aaccgctcct gaaggccctt
    12541 tccttcacgt attattttct gccgcttttg ggaattgccg tggccctgaa tatgcttaaa
    12601 ttgcgcaatg ccgttcccgt catctgcgcc gtatttttag tcgtgtccat gcttctggaa
    12661 tttttccatg tcttgtaagg aaatttcttt gccgttgacc attgatccca tgaagaaatc
    12721 cgacctgccg gaaatcatga agatcgagcg ggaatcgttt ttgtctccct ggacggtggg
    12781 catgttcaag agagaaatct ccagcgatca ttcccattgc ctttgcgcgc ggatccattc
    12841 cgacgggaaa gagatcgttg ccgggtacat tgttttctgg ctgattatcg atgaagccca
    12901 tctgcacagt ctagcggtaa aaaaggaatt ccgggggaag ggaatcgctt ttcgtctgat
    12961 gcagtcgatg aaaaccattg ccggaaaaaa cgacgtttta ttccagacgc tggaagtcag
    13021 agcctcgaat gtcgaagcaa taaagcttta ccggaagtgc ggttttgtag taaaaggagt
    13081 ccgaccgctt tattactcgg acacccatga agacgcgctg atcatgtggg cagatattaa
    13141 tcctgtcagt gaataaaagg attgtgaaga tggccggaaa tatttacatc ggagaaatca
    13201 tcgcaaacga agagattcag gaagactgct ttctcatgaa ggtgcggacc cattcctctt
    13261 ttgaaaatcc gctgccgggt caatttgtga tggtgcgcat cgccgggctg aatgatccct
    13321 ttctatcccg tcccatcagt atttattcat tcactcgcga gacaaattac tgcaccattg
    13381 aactgctcta tcgcgtcgtg ggcaagggaa cgcaggtgat ggcggggctg attgaaggct
    13441 cttacttgga gatcaacgga ccgctgggca acggttttga aacggaaaac gtcaaagaga
    13501 atatcgtttt gatttccgga ggcatcggga tcgctccgct gtcgatgctg atggaaagtt
    13561 tgcggcggac agcccgtcgt tcttcagcgg caataatgat gtatacgggt tttcagaaca
    13621 aaaccgccgt tgtcggcctg gataaaatgc gcggggcttg tcgcgatgtc agcgtctgca
    13681 ccgacgacgg aacgctgggc agcaaaggtt tcgtcacgca gatttttgaa aaagacatga
    13741 aaaattttac accggagaat acgtcgattt atgtctgcgg acccagggaa atgctcaagg
    13801 cgctggcaaa aatattgaat aaaagtaaat tcgactgtca ggtatctttg gaagagcgga
    13861 tggcctgcgg caccggggcc tgcatgggct gcgcggttgc agtaaaagat aaagaaggcg
    13921 catttgctta caaacgggta tgcgccgacg ggccggtttt taatttatcc gatattatct
    13981 gggattaaat tcgatgacga aaacaaaaac aacacaatct ccgaaaatgg ctgtgagcct
    14041 gggacgcctg aaattaaaca atcccgtgat gacggcttcg ggaacgtttg gttacggaga
    14101 ggaatacgcc ggttatgttg atttgaacaa actgggcgca atcgttgtca agggtttgtc
    14161 tttgaaaccc cggctgggca atccgccgcc gcgcatcatg gaaaccaccg gcggaatgct
    14221 caatgcggtg ggtctgcaga atatcggcgt tgacgcgttc atcgaagaaa aaattccttt
    14281 tctgcgccag tacgacacga aagttatcgc caatatttac ggcgaaacct acgatgaata
    14341 taaaaaggtg gcccgaaaac tcagttcggt caagggcatc cacgcactgg aagtcaatat
    14401 ttcctgcccc aacgtaaaaa atggcggtct gagtttcggt tccgatccaa agattgccgc
    14461 ccggatgacg cgcgcggtca aggatgaaac gtctctgccc gtgattgtga aacttactcc
    14521 caatgtgacg gatata
//
LOCUS       DAOSME010000057        13678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_5244_length_13678_cov_17.316230, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000057 DAOSME010000000
VERSION     DAOSME010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5244_length_13678_cov_17.316230"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            600..857
                     /locus_tag="PLG98_08570"
     CDS             600..857
                     /locus_tag="PLG98_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33350.1"
                     /translation="MSEAFTRESIREHHRVKIKKIINQGGGVEEIDNYLMNRISTVIS
                     YERRKNERRQLRKVTKNRSVFSNDEALTEILYLAIRDVMKK"
     gene            1112..2902
                     /locus_tag="PLG98_08575"
     CDS             1112..2902
                     /locus_tag="PLG98_08575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE33351.1"
                     /translation="MTVYKRRLWLLCLVTGIVCHCAHAHALEPTFLKDSPKVNICRAL
                     EFLEDPAGELSLEQLLHPGTQDRFVPLNDPQLNFGITRSTYWFRFLLKFDQSENSCEY
                     ECLDNNSWLLEIDRAWMPTIELWVPVIRDSHDIRKTAQIMGESWRKLSAVTSDQSNRI
                     NILNRSSIFFIPKDYDESRPMYLKMASWGPPLNFSLHVRRLNEFRNWLTWDFTVFGII
                     YGLIIAMTLYNACMYLSLRDKTYLVYVLYIFSNFLYLSIGLGHFSALTGILSGSHFYL
                     IYVFTGSTGFFAAWFCRTFLLTSKNAPLIDKILMAYMFFAILLILMAVTGFYYQVQIL
                     GAAIGLTFPIMAITAGLVCFIKGMKPAGYFLTAWSVLSVGMLTWAFRSLGILPQTQLI
                     TYSYPIAVAAESILLSLALADRIRTLQQENRELENREHRLLILSNTDGLTKLYNKRYF
                     NEKLKSEISLSGNLGDPLSLLILDLDDFKKINDAYGHPAGDQVLKRLGEILLMSIRDK
                     DYGCRYGGEEFTVILPGAEKEKALDVAERIRRLISDDTFETSDGQKFSVTVSIGLAQL
                     RPGENSADLINRADQSLYSAKRQGKNQTVA"
     gene            2951..3373
                     /locus_tag="PLG98_08580"
     CDS             2951..3373
                     /locus_tag="PLG98_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOE33352.1"
                     /translation="MKFLRYIWALPNTTLGLLFVPLALFTRGGMHVVNGVLELHSRLI
                     SLILKKCMPVRGGVSAITLGQNRESLSINRRHEHAHVHVRQYEMLGPIFIPVSEKLIS
                     WLTENQIKVLNVAGPRLRKDPTIYDAVLRLLKVTLSAA"
     gene            3386..3805
                     /locus_tag="PLG98_08585"
     CDS             3386..3805
                     /locus_tag="PLG98_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE33353.1"
                     /translation="MKSFRKELWFNTKKRREFINITSQVEDCLRESGIREGLALINAM
                     HITASVFINDDESGLHQDYENWLENLAPHEPVAHYRHNRTGEDNGDAHLKRQIMGREV
                     VVAVTNGQLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(3939..5204)
                     /locus_tag="PLG98_08590"
     CDS             complement(3939..5204)
                     /locus_tag="PLG98_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HOE33354.1"
                     /translation="MIFKKKTSHGFTFLISFIIVIFAVMFLATDVHALDISNMYSPLN
                     KKRPLRSKTQYIILHTTEGALNGSLRKVHQFGETHYFVTPTGKVYRIIDRKKIATHAG
                     RSMWNRQTTLDNVSIGIEVVGFHDQDITRAQYTALRQLLRQLKKIYRIKDKNILTHSM
                     VAYGRPNIFHPRKHRGRKKCGMIFSHPDVRKLLGLLPGPKRDPDVAAGRLVVADPDLH
                     RFLYKPPKVLAALKGTKDSSGSRSMVIAQGNNAWRIARDKYDAPSTIYTYPNGKKLRG
                     NEIRKWDSIPVGTRVSFVDEDREQEFEKFLEIGKDGNTVQDLAGNLYADPTTIYFFPD
                     GLIRTGAEMKKSSSLRKLLKNPPKGTRLLVGYIYGGYVQKGRSATRIAGRKWNYPSTF
                     YRMPDGRILNGDNVEKKSIPSGTLIFIMK"
     gene            complement(5272..6156)
                     /locus_tag="PLG98_08595"
     CDS             complement(5272..6156)
                     /locus_tag="PLG98_08595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOE33355.1"
                     /translation="MRRTLVGFALSFFSALLLFTFPANTGALSKADVSFSSDTLDRGG
                     VILVTVKTNEQKKPRITWLQKKVPLVYSTSDESWSGFISADLHQETGLYDCMIRETSS
                     GFQERYPIRIRLKDYGVRRLKLPKTKVELDAHSLKRVKNEAAVIHSLWPAGDTSPKWK
                     GHFVMPVDGDIVGTFGKKSIINQLHRSPHTGVDLAGKKGDPVVAINHGRVLLAADHFF
                     SGKSVYVNHGGGIISMYFHLDKILVNKGDDIHKGEVIGLLGATGRVTGPHLHWGVRIN
                     GNRVDPLTLIALSGTLEE"
     gene            6514..7092
                     /locus_tag="PLG98_08600"
     CDS             6514..7092
                     /locus_tag="PLG98_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOE33356.1"
                     /translation="MMENKKDSIPDLAECEDLMVRYAMLPNIVEHSRQVMRVSLAITD
                     HLQEGLSINRDLIVAAALLHDITKTRSLQTGEKHADSGGVLLRKLGFRRVAEIVEQHV
                     TIPDLNLQGGLEEREIVYYADKRVMHNVIVGLDERVDDLIKRYATDEKIRRRILLNKN
                     QALAVERKISGFMKMDIHQAIGKLVAHDRDKP"
     gene            7126..7812
                     /locus_tag="PLG98_08605"
     CDS             7126..7812
                     /locus_tag="PLG98_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOE33357.1"
                     /translation="MERKKLTITIQQGENVSAVLTLPPDKKKHYRAGVIVAHGAGNDM
                     EHEMLVDFSDGLARAGYPSLRFNFPYKEKGLKAPDGQKKLEATWGAVFLHFQTDMPFQ
                     VDHVIASGKSMGGRVASQMAAEKQLPADGLIFLGYPLHPAGDLTKTRDAHLYRLDVPL
                     LFFAGTKDALCDMILFRKVLKNLSAPWQLDVIEGGDHSFHVPKSLGIRQSAIHDRIVK
                     TSGAWLDGQF"
     gene            7848..8303
                     /locus_tag="PLG98_08610"
     CDS             7848..8303
                     /locus_tag="PLG98_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33358.1"
                     /translation="MENIFDKIKSNLKKGMEEGIAVLREGATVVSVRMNELSEEGKRQ
                     YKIFNLNLKIQEQIKELGCMAYAALDKGKRLDDDKKIKAAYSKIKKIEWQLTKTEGQK
                     KVKTPSAGKTAAKPGAKRAKASPKKAKPQSAKAAGKSSAPGTGPKATSG"
     gene            complement(8331..9242)
                     /locus_tag="PLG98_08615"
     CDS             complement(8331..9242)
                     /locus_tag="PLG98_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOE33359.1"
                     /translation="MTLKGILTVVRIPFLILALILGILGAAVAWYESVRYGSPFNLCY
                     AVLATIGLLIAHAAVNIFNDYFDARSGLDYKTNRTPFSGGSGAVPTGLLTVHESLQLG
                     IICFVVLVPIAVFFIMKSGWMLLPLLIAAMLLIVLYTPFILKMGYPEWSPGLGLGILP
                     VMGAYFVHTGQYAPTALVASMPSYFLVHNLLLLNEFPDAEADVTVKRRTLPIVAGKKN
                     AAFFFSGVTLLVYIWVLGAVIWGSMPVFTLVCFLTMPLALQVIRGSFRYDERELLMDT
                     MKKNVLLVLLTQALIAAGYIAAGIFSR"
     gene            complement(9370..10065)
                     /locus_tag="PLG98_08620"
     CDS             complement(9370..10065)
                     /locus_tag="PLG98_08620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33360.1"
                     /translation="MTNPNTLLIRLLTRLRDMRLVWDIMGSLYNGRIYNLIEELYDDI
                     AKDLDTPDQAQILDAGAGRGYLSVALASRNPDSHVTGVDYSRMQVRRAERYRREKKIP
                     NCSFRQNNVLSLDFQNAAFDAAVSVGSIKHWPDSRRGLAELYRVCKPGSCLIISETDR
                     DVPDNELRKFVQRFRLPFVPEDLLFWGLRHVVFGQSFSQDQLADAVRLAGFRNIESHK
                     VKACPYVIVKAWK"
     gene            complement(10134..10691)
                     /locus_tag="PLG98_08625"
     CDS             complement(10134..10691)
                     /locus_tag="PLG98_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOE33361.1"
                     /translation="MIRLEKIISGGQTGADRAGLDFAIDHKMDYGGWVPKGRKTEDGS
                     LPLKYHLQEMPTRDYAMRTLQNVLDSDGTVIVSHGFLTGGSALTREFALKYKKSWLHI
                     DLNALSLPDAAHMLLHWLEEKEIRVLNIAGPKAGKDPKIYKAVLRLLQEALTDSHVLG
                     SGKKSCVKLQCTPSSKPGSSGEKQK"
     gene            complement(10877..12415)
                     /locus_tag="PLG98_08630"
     CDS             complement(10877..12415)
                     /locus_tag="PLG98_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE33362.1"
                     /translation="MKKVVIAGVVIIVLVFSFIGFLHGNNAKPSSPSGAAVSSGMPSG
                     ADTYMLLKNVKFRFAEDIFINVHHMLSITEKIKPYPYVDMNNDQSFSLKIISGHCSLE
                     PPVMETLFNKHVLNYKGAPLKDLKLSTVEMDIDGQKVPMLKTSGMMKLVVWLPFEMIA
                     RVGVDKPTNRMTLEAYKIKSVGLPFVKNLMDMSGVKLSTLLSIEPGRGLSIEKNTMYV
                     DALNITPPPKLVGKLLDAKANVSTNSVDLTLGEEKPANVKYTLLVPDAKNFIYIFQGS
                     LIFGKLLALDANLQLVDANPADYLDFYLKKYLLQLSRSAIRMKPDASLIVNIADYQSD
                     SSPAPAKPAEPAKPTSLSKAAPAKAVSTKPVAGKPAKTPAAKPAASAPAVKQHASINL
                     HLEFDTAQSAVKEQYHSHIKKLADLMKKSPDSRVVIQGHTDNLDKFNNPDNNIKLSQA
                     RADSVRQYLIDKFGINAARIKAIGYGPNQPIADNATLEGQKKNRRVEAVIQTSPQGMV
                     ASTL"
     gene            complement(12616..13038)
                     /locus_tag="PLG98_08635"
     CDS             complement(12616..13038)
                     /locus_tag="PLG98_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE33363.1"
                     /translation="MMIVTKTIKLQTQGFNDMVNITPRVLSLLEDSPVADGLICVFCP
                     GSTGSVTTIEYESGVLHDLKKALERIAPSDIPYRHDERWGDGNGFSHVRAALMKPSLT
                     IPLVGGRPTLGTWQQIVFIDFDNRKRHRELVVQIIGDA"
     gene            complement(13051..13548)
                     /locus_tag="PLG98_08640"
     CDS             complement(13051..13548)
                     /locus_tag="PLG98_08640"
                     /inference="COORDINATES: protein motif:HMM:NF018232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 family protein"
                     /protein_id="HOE33364.1"
                     /translation="MDKIFTKKGVLFGFLLVAFIIVFEIILASLSLPAWPAFMVMIFF
                     FVVHEDTKQGAPIVIGGLAGIFCAVLSEMFIDVLMPVMGHEAAKLLFIGLFVYAIVLL
                     KDAIPYVFNSYAFMFFLVSAIARPSATVSPFVWMAVELVVGVIFILGILGINRIVSAI
                     LTEEK"
BASE COUNT         3647 a         3134 c         3419 g         3478 t
ORIGIN      
        1 ccgcgcacag acgcgagttt atcaacataa caggtcaggt ggaggattgt ctgcgcgaga
       61 gcgggattcg cgaaggactg gccttgatta atacaacgaa agagtacttt tagcaaaagt
      121 tattcctttt acatttaagg gcgaagtcac ggctctgcat tcatttcgta ttaaagaagc
      181 ctttggctat taatggccaa aggcttttct gtttttggag atgagaatga aaaataaaat
      241 aataggcaaa aatggtgttc aaatagtgtc attgaaccga cgaaaggcca ttcacgaaaa
      301 gtaccttaac tgttctgcat ggagcccaaa agatgttgcc ggctgcaaat gaattcttgc
      361 cctctttatt catatcggac gagtaagggg aagcagaatg tcaaagaaag acaaaagtcg
      421 attcgcacat attgcgtcgc ttgcatgaac ggtcatgttg gagcagtttt aagctgccag
      481 tcgtcggatt gtccactttt tatttatcgc aaaggaggtg ttgataaatt ggcaagaatt
      541 tttatgaaga aaaattttaa tcacgaagca gaacctttat taagccatga gtcacgaata
      601 tgagtgaagc ttttacgaga gagtcaatcc gggagcatca ccgtgtaaag ataaaaaaga
      661 ttatcaatca aggtggcggt gttgaagaaa ttgacaacta tctaatgaac agaatctcga
      721 cagtcattag ctatgaacgt cggaaaaatg aacgcaggca actgcgcaag gtgacaaaaa
      781 accggtctgt tttttccaat gatgaggcgc tgaccgaaat cttatatctg gcgattcggg
      841 atgtcatgaa aaaatgaacg atgcctttgg ccaactgggc tttgacaatt tctcagctgg
      901 ctgttatgta cgagggccgt tttgatttgg cggcaatatg aaaaattatc catttacaca
      961 gattagttta cagtctcatt ttgaaaattg tgctgccttt caattcgtaa tcaataaagt
     1021 tgcaccccat atcttgtggc agaatattct tgactctcaa gaaaccgttt cctatactgc
     1081 cgacagtaaa aaggaagttc taatcaattc aatgacggtg tataaaagaa gactatggct
     1141 gctgtgtctt gtcacaggta tcgtgtgcca ttgcgctcat gcccatgctc tggagccaac
     1201 atttctaaaa gattccccaa aagtaaacat ctgccgggct cttgaatttt tggaagatcc
     1261 tgccggagaa ctgtcgttgg agcaactgct tcacccgggc acgcaggatc gttttgtccc
     1321 gttaaatgac ccgcagttaa atttcggtat cacccgatcc acgtactggt tcaggtttct
     1381 gctgaagttc gatcaatccg aaaattcatg cgaatatgag tgcctggata acaattcatg
     1441 gctcctggaa attgaccgtg cctggatgcc gacaatcgag ctgtgggttc cggttatccg
     1501 ggatagccat gatattcgga aaacggcaca aatcatggga gaaagctgga gaaaactttc
     1561 tgccgttaca tctgatcaat caaaccggat aaacatctta aaccggagtt ccatattttt
     1621 tatccccaaa gattatgatg aaagtcgtcc catgtatctt aaaatggcgt cctggggacc
     1681 gccgctgaat ttttcgctgc atgttagacg attaaatgaa ttccgcaact ggcttacgtg
     1741 ggatttcacc gtttttggca ttatttatgg attgattatt gccatgaccc tttataatgc
     1801 atgcatgtat ttgtccctcc gggacaagac atatctggtg tatgtattat acatattttc
     1861 aaacttcctc tacctgtcca tagggctcgg tcatttcagt gcgcttaccg ggatactgtc
     1921 gggatcacat ttttatctta tttacgtatt caccggttct actggttttt tcgccgcctg
     1981 gttctgccgt acctttctgc tgacttccaa gaatgcgccg cttattgata aaatattaat
     2041 ggcgtatatg ttttttgcta tcctcttgat attaatggct gttacgggtt tttattatca
     2101 ggtgcagatt ttaggcgccg ccatcggctt aacctttcca atcatggcta tcaccgccgg
     2161 cctggtatgt tttatcaaag gcatgaagcc ggccggatat tttttaactg catggtcggt
     2221 cttatccgtc ggtatgctga cctgggcgtt tcgcagcttg ggcatcctgc cccaaacaca
     2281 gttaattacc tattcttatc cgatcgcggt tgccgcggaa tccattttac tctccctggc
     2341 cttggcggac cggataagaa cgttgcagca ggaaaaccgg gaactggaaa acagggaaca
     2401 ccgtctgttg attttaagca ataccgacgg ccttacaaaa ttgtacaata aacggtattt
     2461 taatgaaaaa ctaaaaagtg aaatctccct ttccggtaat ttaggcgatc ccctgtcatt
     2521 gttgatactg gatctggatg atttcaaaaa gataaatgac gcgtacggac accctgccgg
     2581 ggatcaggtg ctgaaacgac tgggcgaaat ccttctgatg tcgattcgcg ataaggatta
     2641 cggttgcaga tacggcggag aggagttcac cgtcatcctg ccgggagccg aaaaggaaaa
     2701 agccttggat gttgcggaaa gaatccgccg cttaatcagt gatgatactt ttgaaacaag
     2761 cgacggacaa aaattttcag tgactgtcag catcggtctt gcccagctcc gtcccggtga
     2821 aaacagcgcg gatttaatta atagagccga ccagtcactc tatagtgcca aacgccaggg
     2881 caaaaaccaa acagttgcat aacaaccgct tatttgtgag taccgggaat tctggggaca
     2941 aggcgttaaa atgaaattcc tgcgatatat atgggcactg cccaacacga cgctgggact
     3001 tctgtttgtt ccgctggctt tgtttacccg tggcgggatg catgtggtta acggtgtgct
     3061 cgagctacac agcaggctca tatcgctcat ccttaaaaaa tgtatgcctg tacgtggcgg
     3121 cgttagcgcg ataaccctgg gacaaaacag agaatctctt tccatcaatc gtcggcatga
     3181 gcatgcgcat gtgcatgtgc ggcaatatga aatgttaggg ccgatattta ttccggtctc
     3241 tgaaaaactg atttcctggc tgactgaaaa ccaaatcaaa gtcctcaatg ttgcggggcc
     3301 aagattacgt aaagacccaa cgatatatga tgctgtattg cggctgttaa aggtaaccct
     3361 ttcagctgct taatggaggc aaagaatgaa gtcgttcaga aaagaacttt ggttcaatac
     3421 caagaaaaga agggaattta tcaatatcac ttcacaggtg gaggattgtc tgcgtgaaag
     3481 cgggattcgc gaaggactgg ccttgattaa cgccatgcat atcacggcgt cggtgttcat
     3541 caacgatgac gaatccggtt tgcaccagga ctacgaaaac tggctggaaa acctggcgcc
     3601 ccatgagccc gttgctcatt accgtcataa ccggactggc gaggataacg gcgatgcgca
     3661 tttgaaaaga cagatcatgg gacgggaggt tgtcgtggcg gtgacaaacg gtcaactgga
     3721 cttcggaccg tgggaacaga ttttttacgg cgagttcgac ggaagacgca gaaaacgggt
     3781 gctggtgaaa atcataggcg agtaatgcat ctttttgcct ccgttgacag ttgaccgtga
     3841 cggcggttac cgcgtcttgg ttcgtcctcc agccttatca aggattcacc gggcatgtga
     3901 tccgtttcag ccgcagcatt cttccgggta aaaacggttt atttcatgat gaagatcagg
     3961 gttccggaag gaatggattt tttctctacg ttgtccccgt tgagaatgcg cccgtcaggc
     4021 attcgataga atgtcgatgg ataattccat tttctgccgg cgatccgggt ggcggatcgc
     4081 cctttttgaa cgtacccgcc gtaaatataa cccaccagca aacgggttcc tttgggcgga
     4141 tttttcagga gcttgcgcag agacgacgat ttcttcattt ctgcgccggt tcggatcagt
     4201 ccgtcgggga aaaaatagat tgttgtcgga tcggcgtaaa gattgccggc cagatcctga
     4261 accgtgttcc catctttgcc gatttccagg aatttttcga attcctgctc ccggtcttca
     4321 tccacaaatg aaacgcgggt acccacaggg atgctgtccc atttgcggat ttcattgccg
     4381 cgcaatttct ttccgttggg ataggtataa attgtcgagg gggcatcgta cttatcccgg
     4441 gcaatgcgcc aggcattgtt cccctgggca ataaccatgg atcgactgcc ggatgaatcc
     4501 ttcgttcctt tcaaagccgc caacaccttc gggggtttgt agagaaaacg gtgcagatcc
     4561 ggatcggcaa caacgagccg gccggcggca acgtccggat ctcttttggg tccgggcaag
     4621 agccccagaa gttttctgac atcgggatga gaaaaaatca ttccgcattt ttttcggccc
     4681 cgatgttttc ggggatgaaa aatgttcggc cgtccataag ccaccatgga gtgcgtcaga
     4741 atgtttttat ccttgatccg gtaaatcttt ttcaactgac gcaatagttg acggagcgcc
     4801 gtgtactgcg cccgggtgat gtcttgatcg tgaaacccga caacttcaat gccgatggaa
     4861 acattgtcaa gggtggtttg ccggttccac atgcttctgc ccgcatgggt ggcgattttt
     4921 ttgcggtcaa tgattcgata tacttttccg gtgggcgtaa caaaatagtg cgtttccccg
     4981 aactgatgaa ccttgcgaag cgaaccgttc agagcgcctt ctgtcgtatg caaaatgatg
     5041 tattgggttt tcgaacgcaa cggacgtttt ttattcaaag ggctgtacat gttgctaatg
     5101 tccaaagcat gaacgtccgt cgccaaaaac ataaccgcaa aaataacgat gatgaacgat
     5161 attaaaaatg taaatccgtg cgacgttttc tttttaaaaa tcattgattg tccaatggcc
     5221 atctttatcg attcattgct ttgtgctttc ccgaaaaaac gtatcatcca tttattcttc
     5281 aagagtgccg ctcaatgcga tcaacgtcag gggatcgacc cggttgccgt tgattctcac
     5341 tccccagtga agatgaggac ccgtaacgcg tcccgttgca cccaaaaggc cgatgacttc
     5401 gcccttgtga atatcatctc ctttgtttac caatattttg tcaagatgaa aatacatgct
     5461 gatgatgcct ccgccatggt tcacataaac cgatttgccg ctgaaaaaat ggtcggccgc
     5521 tagaagaacc ctgccgtgat tgatggcgac aaccggatcg ccttttttgc cggccaggtc
     5581 cacaccggta tgcggcgatc tgtgcagctg gttaataata cttttcttgc cgaacgtgcc
     5641 cacgatatct ccgtcaaccg gcatcacgaa atgccctttc cattttggcg atgtatcgcc
     5701 ggccggccat aacgaatgaa tgaccgcggc ctcgtttttt actctcttta aggaatgagc
     5761 atccagttcc actttggttt tgggaagttt caatcggcga acgccgtaat cttttaaacg
     5821 gatacggatc ggatatcttt cctgaaagcc ggaagaggtc tcccggatca tgcagtcata
     5881 taatccggtt tcctgatgca ggtccgcgct gataaatccc gaccatgatt cgtccgacgt
     5941 gctgtatacc aggggtactt ttttttgcag ccatgttatt cggggctttt tttgctcgtt
     6001 cgtctttacc gtgacaagaa tcactccgcc ccggtccaac gtgtccgatg aaaaggacac
     6061 gtccgctttg gacaacgccc cggtgtttgc cggaaatgta aaaagcaaaa gcgctgaaaa
     6121 aaaagataat gcaaatccga ccaatgtcct tctcaattgc gttatttcct caaaaagtta
     6181 ttcctgtgcg ttagacagcg caaaacggga taaaaaaaat cgctcacccg acttatgcca
     6241 gatggcgacc caaccatcga aaaatctcct caagtcctga tgactttttg gaccccattc
     6301 gatatttttc tttttaccta tcatgtatgt gataaaaatg caagttgcga tagccgttta
     6361 cactgacaaa atcaaaaata ttcttcggtg ctacttgttt aaaaagtgtg gaagggttta
     6421 ggcgcccggt gtattttctt ccttgcatcc tcttttaaaa ccgaacaggt tttttgattg
     6481 acattattta taaaaaatag aaatcttaaa ctgatgatgg aaaataaaaa agatagcatt
     6541 ccggaccttg cggaatgcga agacctgatg gtccggtatg ccatgttgcc gaacattgtg
     6601 gagcattcca gacaggtaat gcgggtttcc cttgccatta cggaccatct tcaggaggga
     6661 ctgtcgataa atcgggattt gattgttgcc gcagcgctgc tgcatgacat taccaaaaca
     6721 aggtctcttc aaaccgggga aaaacacgct gactccggag gggtgctttt gcgaaaactg
     6781 ggatttcgac gtgttgccga gattgtggag cagcatgtca cgataccgga tctgaatctt
     6841 cagggcggtc tggaagaaag ggaaatcgtt tattatgccg acaagcgggt gatgcacaac
     6901 gttattgtcg gcctcgatga gcgggtggat gatctgatta agcgatatgc caccgacgag
     6961 aaaatccgcc gccggattct tctcaataaa aatcaggcgc tggccgtcga acggaaaatt
     7021 tccggtttta tgaaaatgga tatccatcag gccatcggaa aacttgttgc acatgatcgg
     7081 gataaaccgt aaaatcatca gggaaaacag acgcggaaat aatctatgga aagaaaaaaa
     7141 ctgaccatca ccatccagca aggtgaaaac gtttcggcag ttctgactct gccgccggat
     7201 aagaagaaac actaccgcgc cggcgtgatc gttgcacacg gtgccggaaa cgatatggaa
     7261 catgaaatgc ttgtcgattt cagtgacggg cttgcccggg cggggtatcc gtcgctgcgt
     7321 ttcaattttc cctataaaga aaaaggcctg aaggcgccgg acggtcaaaa aaaactggaa
     7381 gcgacctggg gcgcggtatt tctgcatttc cagaccgata tgccttttca ggtcgatcat
     7441 gtcattgcgt ccggaaaatc gatgggcgga agagtggcct ctcaaatggc cgcagaaaaa
     7501 caattgccgg ccgacgggct tatttttctg ggatatcctc tgcatcctgc cggcgacctg
     7561 accaaaacaa gagatgcgca tctgtaccgc ctggacgtgc cgctgctttt ttttgcgggg
     7621 acgaaagatg cattgtgcga tatgattctg ttcaggaaag ttctgaaaaa tctgtctgca
     7681 ccttggcaac tggatgtgat cgaaggcggc gatcattctt ttcatgttcc gaaatccctg
     7741 ggcatccgtc aatcggcaat tcatgaccgc attgtaaaaa catccggagc gtggctggat
     7801 ggacaattct gaaacccggc acattaaaag gagataagga ggaaaccatg gaaaatatat
     7861 ttgataagat caagtccaat ttgaaaaaag gcatggaaga ggggattgcc gtcttgcggg
     7921 aaggcgcaac cgtcgtgtcc gtgagaatga atgaactttc ggaggaagga aaaagacaat
     7981 acaaaatttt caatcttaac ctgaagatac aggagcaaat taaggaactg ggctgtatgg
     8041 cttatgcggc gcttgataaa gggaaacgtc tggacgacga taaaaaaata aaggcggcgt
     8101 attcgaaaat caaaaagatc gaatggcaac tgaccaaaac cgaagggcag aaaaaagtga
     8161 aaacaccttc cgcgggaaag acggcggcca aaccaggcgc gaaacgcgcc aaagcgtcac
     8221 cgaaaaaggc caaaccgcag tccgccaagg cggccggtaa atcgtcggct ccggggaccg
     8281 ggccgaaggc aacatccgga tgaccaggtg ctttaaaggg ttgatccgat ttaacgagag
     8341 aagatgccgg cggcaatata ccctgccgca atgagcgcct gggtcagcag cacaagcagc
     8401 acgttttttt tcatggtgtc catcaggagt tctctttcgt cataacgaaa agaacctcta
     8461 ataacctgaa gggccaacgg catcgtgaga aaacacacaa gtgtaaagac gggcatagac
     8521 ccccaaatga ctgcgcccag gacccagata tagaccagca gagtgacacc cgagaaaaag
     8581 aaggcggcgt ttttctttcc ggcaacgatg ggcagtgtcc gtcttttcac cgtgacatcc
     8641 gcttcggcgt cgggaaattc attgagcaga agcagattgt gcaccagaaa atacgacggc
     8701 atggaggcaa ccagcgcggt cggagcatat tgtccggtgt gtacaaaata agcgcccatc
     8761 acaggcagaa tgcccagtcc caatccgggc gaccattcag gataacccat ttttaaaata
     8821 aacggcgtgt ataaaacaat caacaacatg gctgcaatca gcagcgggag aagcatccat
     8881 ccgcttttca tgatgaagaa gacggcaatc ggtacaagga cgacaaagca gataatcccc
     8941 agttgaaggc tttcatgtac cgtcagtaat ccggtcggaa cggcgccgct gccgccgctg
     9001 aaaggcgtgc ggttggtttt ataatccagg ccgctgcggg catcgaaata atcgttgaaa
     9061 atattgaccg ctgcgtgggc aatcagcaac ccgatggtgg caagaaccgc atagcagagg
     9121 ttgaacggcg agccgtaccg gacggattca taccaggcga cggcagcgcc gagaatgccc
     9181 agtatcagag ccagaatcag aaagggaata cggacaaccg tgagaatacc ttttaacgtc
     9241 atggaatatt tttcatcctt tgccgaaatg attcggattg tcgtttaaca aaatattgat
     9301 cgatctggca agcatataat gaaggccgga ggaatggatg tgaaaatcag ccaggaaggg
     9361 ttattggttt tatttccatg cttttacaat gacgtaggga caggctttta ccttgtggct
     9421 ttcgatattg cggaatccgg ccagacggac ggcgtcggcc agttgatcct gagaaaagct
     9481 ctgtccgaaa accacgtgac gcagtcccca gaaaagcaga tcttccggca caaaaggcag
     9541 gcgaaagcgt tgaacaaatt tgcgcagctc gttgtccggg acgtcgcggt cggtttcgga
     9601 aatgatcagg cagcttccgg gtttgcagac gcggtaaagt tctgccagac cccggcggga
     9661 atccggccag tgtttgatcg atccgacact caccgccgca tcgaaggcgg cgttctgaaa
     9721 atccagagac aggacattgt tctggcggaa agagcagttc ggtatttttt tctcccggcg
     9781 gtagcgttca gcccggcgaa cctgcatccg ggagtaatcg actcctgtaa catggctatc
     9841 cggattccgg gatgccagcg ccacagacag gtatccccgg cccgcgcccg catcgagaat
     9901 ctgcgcctga tccggagtgt caagatcttt ggcgatatcg tcgtagagtt cctcgatcag
     9961 attatagatg cgtccattgt aaagggatcc catgatatcc cagacgagac gcatatcgcg
    10021 cagccgagtt aaaagtctga tcaacagggt attgggattg gtcatcggat agcgtccttt
    10081 acgttaaacc gtttttcctt gttaacagtt tctcgttccg ttttcgcaag tgtttatttt
    10141 tgtttctccc cggacgatcc cggcttcgaa gacggcgtgc attgaagctt gacgcatgac
    10201 tttttgccgg agcccaaaac gtgggaatcc gtcagcgcct cctgaagcag ccgcagaacc
    10261 gccttatata ttttcgggtc tttgccggct ttgggaccgg cgatattgag cacgcggatt
    10321 tccttttcct ccagccagtg taaaagcata tgggcggcat ccggcagaga taatgcgttc
    10381 agatcaatgt gaagccagga ctttttgtat tttagggcga attctctggt taatgccgaa
    10441 ccgccggtga gaaaaccgtg agacacaatg accgtgccgt cgctgtcgag cacattctga
    10501 agggttcgca tggcgtaatc gcgtgtcggc atttcctgaa gatggtattt cagcggaagc
    10561 gaaccgtctt ccgtttttct gcctttggga acccatccgc catagtccat tttatgatcg
    10621 atggcaaaat cgagtcccgc tctgtccgct cccgtttgcc cgccggatat tatcttttcc
    10681 aacctgatca tcgattttca acccgactct ttttgagtaa tttaaatcat ttttaccaca
    10741 tgtcaaaggg gcgttcggtt tattttgctc gagcaacaaa ccaaaatacg gtcatgtgaa
    10801 cggggattac cggtaaagaa aaacagcgac agggcgtatc cggtggatag ccttgtcgct
    10861 gttttatgaa acggggctaa agggttgatg cgaccatccc ctgcggcgat gtttgaataa
    10921 cggcctcgac cctccggttt ttcttctgtc cttccagagt ggcattatcg gcaatcggtt
    10981 ggttcggtcc gtagccgata gccttgatgc gtgcggcatt gatgccgaat ttgtcgatca
    11041 gatactgacg aacactgtcc gcccgggcct gagacaattt tatattattg tcggggttat
    11101 tgaatttgtc gagattatcg gtatgcccct gtatgacgac gcgagaatcc ggtgattttt
    11161 tcattaaatc ggccagtttt ttgatatgag agtgatattg ttctttaaca gcggattgag
    11221 ccgtatcgaa ttccagatgg agattgatcg aagcgtgctg cttgaccgcg ggcgccgaag
    11281 cagcgggttt agcagcgggc gttttggccg gttttcccgc aacgggtttt gtcgaaaccg
    11341 ccttggccgg agcggccttg gataacgatg tgggcttggc cggttccgct ggttttgccg
    11401 gagcgggcga tgaatcggat tgataatcgg caatattgac tatcagcgaa gcgtccggct
    11461 tcatccgaat ggcacttctg cttaattgta ataaatattt tttcagatag aaatccagat
    11521 aatcggccgg attggcatca accagttgca ggtttgcatc aagcgcaagc agttttccga
    11581 agatcagtga cccctggaag atatagataa agttttttgc gtccgggacc agaagggtat
    11641 atttgacgtt tgccggtttt tcctctccaa gtgtcagatc gacggagttt gtactgacgt
    11701 ttgccttcgc atcaagaagc ttgccgacca gtttcggcgg cggggtgatg ttcagcgcgt
    11761 cgacatacat ggtgtttttt tcaatggaga gaccccggcc gggttcgatg gataaaaggg
    11821 tggaaagttt gacgccggac atatccatca ggtttttgac aaatggcagc cccacggatt
    11881 ttattttata ggcttccagt gtcatccggt tggtcggctt gtccacgccg actctggcaa
    11941 tcatttcaaa cggaagccag acgaccagtt tcatcatgcc gcttgttttc agcatgggca
    12001 ctttctgacc gtcaatatcc atttccaccg tcgacagctt aagatctttc agcggtgcgc
    12061 ctttataatt caaaacgtgt ttattgaaga gagtttccat caccggcggc tcgagcgagc
    12121 aatgtccgct gataatttta aggctgaagg attgatcatt attcatatcc acgtacgggt
    12181 aaggtttgat cttttcggtg atcgacagca tgtggtgaac gttgatgaaa atatcttcgg
    12241 caaatctgaa tttgacattt ttcagaagca tgtaggtatc agctccggac ggcatgcctg
    12301 acgataccgc cgcgccggaa ggtgatgaag gctttgcatt gtttccatga aggaaaccga
    12361 taaatgaaaa aacaagaaca atgatgacga caccggctat gacaactttt ttcataggca
    12421 ccccctaaaa gcaaaagaat aggaaattaa tcaaaaaacg gtgaaacttt gatttctgag
    12481 gtttgcagcc taccacaaaa acaaaaaatt caaaacagag aattcgaggc ggctccctgc
    12541 acaaagaaac tcgtgaaagc aaatcgggaa tgtctgtcaa acggtcgcat cgggcacgat
    12601 gcaggtcgaa tcgaattatg catccccgat aatctgcaca accagttccc ggtgtcgttt
    12661 tcggttgtca aagtcgataa agacaatctg ctgccaggtt cccagcgtcg gcctgccgcc
    12721 gaccagcgga atcgtcagag acggtttcat cagtgcggcg cggacatgcg aaaagccgtt
    12781 gccgtctccc catcgttcat cgtgccggta agggatgtcc gacggggcaa ttctttcgag
    12841 tgcctttttc aaatcatgca gtacgccgga ttcatactca atggtcgtca ccgaaccggt
    12901 ggagccgggg cagaaaacgc aaatcagccc gtcggcaacc ggcgagtctt ccagaaggga
    12961 aagaacccgg ggagtgatat tgaccatatc gttgaacccc tgggtttgaa gtttaattgt
    13021 ttttgtaaca atcatcatgc aacgagcttt ttatttttct tccgtcaaaa tcgcggagac
    13081 gatccggttg atccccagaa tgcccagaat aaatataacg ccgacaacga gttcgacggc
    13141 catccaaaca aagggcgaaa ccgttgccga cggccgggca atcgcggaga cgagaaaaaa
    13201 catgaaggcg tagctgttga aaacgtaagg aatggcgtcc ttcaaaagca caatcgcata
    13261 gacgaacagg ccgatgaaaa gaagtttggc ggcttcatgt cccataacgg gcatcaggac
    13321 gtcaatgaac atttcactca gaaccgcgca aaagataccg gccagtccgc cgatcacgat
    13381 aggagccccc tgttttgtgt cttcgtgtac cacaaaaaag aaaatcatca ccatgaaggc
    13441 cggccaggcg ggaagagaaa gggaggccag aatgatttca aaaacgatga tgaaagccac
    13501 cagaagaaaa ccgaacaaaa cgcctttttt tgtaaatatt ttatccatgt atccctaccg
    13561 aatccttaat gttttgagga agaagaataa ggaattcaac agaatttgtc aagcagagag
    13621 gaaagatgcg gaatagaatt gattttgaaa aaagtaattt tatgctaaat gaaagatg
//
LOCUS       DAOSME010000058        13636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_5262_length_13636_cov_19.692732, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000058 DAOSME010000000
VERSION     DAOSME010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5262_length_13636_cov_19.692732"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..681)
                     /locus_tag="PLG98_08645"
     CDS             complement(163..681)
                     /locus_tag="PLG98_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33365.1"
                     /translation="MNTNEDIERFRKLAETGDADAQYHLGLIYQNEKGVSLDYAQAVK
                     WLVMAAEQGHADAQYNLACIYNNGKGVSLDYPKAIKWFTRAAEQGHAHAQYMLGLMYS
                     SGLQDYVEAARWFLKAAEQGIADAQFKIGVILEEGRGVRKDPASAEVWYGEAAEQGHA
                     DAKKRLDKMQNK"
     gene            921..1346
                     /locus_tag="PLG98_08650"
     CDS             921..1346
                     /locus_tag="PLG98_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOE33366.1"
                     /translation="MNTKIGLSQILLYFLMLMFMMVIGCGDGADDFKLPKYHDADADK
                     CTISGRVTYKGVGRAGITVLLSGYSNATVATDENGEYRFDDVDGDQAYVVSPSSGTLI
                     FTPESRGVEPDGSNVDGVDFEAMDCCGDDTSEEEETEEI"
     gene            complement(1493..2764)
                     /locus_tag="PLG98_08655"
     CDS             complement(1493..2764)
                     /locus_tag="PLG98_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE33367.1"
                     /translation="MIRHISSTTFPPKDHYGVDILPLSFFENADYPGSPPIECLVLYK
                     KKTSLPAARESFLETIRHYNLFSSRLIMIDENRFALLYCTDGFQYMVLPPLDADAAHI
                     SIDDVRHMMRHVKTLPGEPLFAVTIIPVRDGLLGGISCSHAIGDAVSLMLFLYAWGCI
                     HYGNSFSKPSTQRLFSGSPVVFDTFDSVFAPPVSDLHETIRKRVENSQETTRYHRREY
                     FSDKRLAEMKNGATSENENYRLSNNQIINAVLLKKYHNGIMPGTDQIRLRIPINLRYI
                     HPDVDPLYIGNAVFSAVIGFSRAEINRMSVYRIACRLKEAIAKMRNEHYVRRIVCLSK
                     FGIRYRGDIFKTLPPYNPDTDILAANLTHVNDLESLGLGDDVGRILYMGSTMQTSFTM
                     LKEKHNRLFAEIRSRYPLESPTTTHGTAEVP"
     gene            complement(2792..5044)
                     /locus_tag="PLG98_08660"
     CDS             complement(2792..5044)
                     /locus_tag="PLG98_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE33368.1"
                     /translation="MLKLFPPDDPLQSVRIKRFLMAFASYSVWLTIALITYLLGIAPV
                     SFHVLFICFMGILLCNILIYAAIRSGFNKRFDDPSLTLFQMIIATFWAMVILYYADDA
                     RGTVLILYLVVFVFGLFKLNLRQFLYLSVFAVLNYALVLFLLYKNRPESLNTENEILG
                     LIVLALVLPWFSFMGGYITRLKKKVTHSLSQAKEMELKFRTIFDSASDGITMLDTRQG
                     TFVAANVTLCQMLGYTQEEFLALKISDLHPKEQQKDILSIYNKINHHELSIARNIPIL
                     KKDQTLLYADISGSLISLEGKEYSVGVIRDITERKFAEELLIASEQRYRLLADNVTDI
                     IFTMDMNLRYTYLSPSGYRISGYNEDEIARTNISDMVEPETYFRFAEVLKEELALDRQ
                     PGRDLKRSRTLEYQHIFKDRSKKWMEATLTFLRDKNGEAVGIIGIARDISERKAMEEK
                     LQFEERRFRNFVEHSADIIVLVNLEGLITYINPAIERVLGYKTEERIGRRGFELVHPD
                     DLEFLIQSFTTLATQPDLPPIHGEMHLRHKNGTYRTLEAVGSNLVKDHVVEAIIINYR
                     DITERKLAEEALQRSEQRYLELSILDDLTQVFNSRHFHAELGKEIERSTRYDHPLSLL
                     LLDLDHFKTFNDTYGHVEGDNVLSRLGQVVKKCLRDSDSAYRYGGEEFTIILPMTTKE
                     DAVSTAQRIQNEFRKEVFTPLQGKEIFVTMSIGASQYIPKEEMKSFVHRVDQFMYQAK
                     QNGRDRICSD"
     gene            complement(5130..>5316)
                     /locus_tag="PLG98_08665"
     CDS             complement(5130..>5316)
                     /locus_tag="PLG98_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33369.1"
                     /translation="AGKSNDDTGIITFFFQFMRHDDDPRVDVKCCTNCYPFFNSTDTK
                     ILPYILPEGVRRIHRSF"
     assembly_gap    5317..5416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5417..5623
                     /locus_tag="PLG98_08670"
     CDS             <5417..5623
                     /locus_tag="PLG98_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOE33370.1"
                     /translation="SDMFSFKSPSFKALGLNPAGLHDKDLMNLMLSEPRLIRRPVVKI
                     GRHVYFGADIKVLSEIITQPVENG"
     gene            5643..6116
                     /locus_tag="PLG98_08675"
     CDS             5643..6116
                     /locus_tag="PLG98_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOE33371.1"
                     /translation="MPKKTSAGIVLYRFVGEDMEVFLVHPGGPFWAKKDKGAWSIPKG
                     LFEPGEDPLEAARREFHEETGHAVSGEFRALMPLKQPSGKVVHAWAVEGHIDEKSIKS
                     NMFSMEWPPRSGKEQSFPEVDRGGWFTLRIACEKILPGQRGFLEELQTKIKNRPA"
     gene            6252..6713
                     /locus_tag="PLG98_08680"
     CDS             6252..6713
                     /locus_tag="PLG98_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HOE33372.1"
                     /translation="MDQKIICLSVGLSQKEIEKGKALYFAASEGQSTLEVTAMSRSML
                     NMKIIEILACLMEELEKNPAEKQNDSADVIPADHCKAAVLVMLAQDRELVLQVMRSFK
                     AVLSDSRNVIFAVITETALKWTLAEYLEHLSREHEYMQTSRPENNPDMKKL"
     gene            6738..8045
                     /locus_tag="PLG98_08685"
     CDS             6738..8045
                     /locus_tag="PLG98_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE33373.1"
                     /translation="MIYNEEYETLPREALEALQLKRLQQVVNRVYHHVGFYKKSFDRA
                     GVHPNDIRTLDDLKRFPFTTKQDLRDNYPFGMFATPMSNIVRLHASSGTTGSSTVVAY
                     TKRDIENWAELVARCFAAADLTRGDIIHNAYGYGLFTGGLGVHYGAEKLGASVIPMSG
                     GNTKWQLMILQDFGPTAICCTPSYALNLAEQGRAMGIDMPSLKLRVGIFGAEPWSEQM
                     RAQIEEAFAIKAMNIYGLSEIMGPGVSMECSVAQAGMHVFEDYFLVETIKPDTGEVLP
                     PGEQGELVFTTLTKEAFPLIRYRTRDISRLITTPCRCGRTFHRMERVMGRSDDMLIIR
                     GVNVYPSQIESVLINIEGLEPHYQLIIERSGTLDTLEVQVEVTEGVFAEAGEVKGLQQ
                     LEKRLIKDMKDYLGITAKVKLVEPKSIMRSEGKASRVIDKRKI"
     gene            8102..8863
                     /gene="hisF"
                     /locus_tag="PLG98_08690"
     CDS             8102..8863
                     /gene="hisF"
                     /locus_tag="PLG98_08690"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOE33374.1"
                     /translation="MKPIKIMPCLDMKNGRVVKGVHFVDIREAGDPVENAEFYQAEGA
                     DELAMLDIAATLENRKTRLEWVKNVSKVIRIPLTVGGGIASLEDIDMVLGAGASKVSM
                     NSAAVKNPELIRQAAEKFGKEKITVAIDAKRNAQMPSGFELVVSGGTMPIARDAVAWA
                     KICQEKGAGVILPTSMDGDGTKAGYDIPFTRAIADAVTLPVVASGGAGKLEDFYEAVV
                     QGGATVLLAASVFHFRLLKIQDVKTYLREKGLAVA"
     gene            8875..9486
                     /locus_tag="PLG98_08695"
     CDS             8875..9486
                     /locus_tag="PLG98_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33375.1"
                     /translation="MSKKNNAGFMYLALLGAAITTLCDGVHVYTQTLSYTTPFLFGQA
                     WWVFPGFVLAFLFMGGVYFGLVNRLPLFFAVKKSISGGSLRDVVETATAFALIYVMSG
                     LGSNEPLLLSAVFYGTFVLRLAFSYDRLFLLLLAVLMAFGGMFVESLYAAAGLMAYRY
                     PDVFYVPFWLGGLYVHGAFALREGMRFFVYDRKPGTLISVSPL"
     gene            9633..10832
                     /locus_tag="PLG98_08700"
     CDS             9633..10832
                     /locus_tag="PLG98_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE33376.1"
                     /translation="MMTEDKKTSWRNKKSDLRRLVLLMNRQNNRFWPPLDPILKTVDL
                     VIEPEELNLLLKMGTDLYTYDQVAALSGINSEKFDAVFDSLKKKAFIGIKNPETSGEQ
                     YTLHPFVVGWFEGQVSYLIGKPEEKEFAKRYMGFLDSLRKYNFFPLRNIMNVIGKHAT
                     VSNQSVGTVRESRNGQGKSKIVVDENIVVPDSGIYPTSSVNDLIMEFGTRSIIGQFKV
                     CMCRQMTANVDQPCRLKMPDDVACMGFGEGIKTYMKYGYMRRISKEQAFDVIQKARDH
                     GAVHTVFHEMDDTKLPQIGLCNCCWDCCGIFRTYNMGVVPLRYRCYYIARIANSDRCT
                     GCGRCEKFCPTSAINVVAKSVVLDTKKCIGCGQCVHQCARAVVELVEDRRTAFLPMLK
                     TSEARLL"
     gene            10878..11501
                     /locus_tag="PLG98_08705"
     CDS             10878..11501
                     /locus_tag="PLG98_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOE33377.1"
                     /translation="MPGLKKKQHLPDDLKKCVEFHGHICPGLIYGYRVAQEAIRLMHI
                     GRSVDEEVVAVCENDSCAVDALQVMLGTVAGKGNLLIKNYGKNAFTVMSRVNRKAYRF
                     SRKKQYAYGGSSGRRFKKLERAVASGDASEEEKQELKRLKIDDLLARPFSDIFTTEEV
                     VFDEPGYAPLAPSEPCAVCGEMTMATKMIPLRDGRQVCIPCSEKSKH"
     gene            11565..11864
                     /locus_tag="PLG98_08710"
     CDS             11565..11864
                     /locus_tag="PLG98_08710"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOE33378.1"
                     /translation="MKKIGITILVVLGVLMTSIAYAANLYGIVSDKNGKPVEVKVTLK
                     DDKGAAVGQPVTTDKKGAYAFKDIKPGSYVVVVGAKDEWKIFVGPGETRRDFSLK"
     gene            11888..12916
                     /locus_tag="PLG98_08715"
     CDS             11888..12916
                     /locus_tag="PLG98_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33379.1"
                     /translation="MAERDIQKQEEPTEAPRKKDLWDILQALGPALIAAAVAVIGSVY
                     SYQQAQMTKASEMREVYTKIMTERENSDNNIRASMFQMLFNAMFDKDINSADTPDNVN
                     QIKKRVMFLDVLSRNFDTVDIKPLFEDLDGELTRKMYNDRSYSDGAQEDYFALRSELR
                     RIGRNLAVKQLNALASLPGTMVKTIVLIEDKDKNIMVMQDETTANGNGDTLPVEIKAQ
                     NLDDGKVEIAIDYQKAKASDKGFKAPSFIITFYDMPYIDNSVLDKDMRVGVVLTKYAT
                     TREIDLFRDRIDRRLLKDYEDLKAGGIAKYAELRLIKFPAKYTGHRDRPYLQEILQNL
                     VENEKSVR"
     gene            13013..>13636
                     /locus_tag="PLG98_08720"
     CDS             13013..>13636
                     /locus_tag="PLG98_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HOE33380.1"
                     /translation="MKKYVPVLLLLIFVFACWACVPVPVPVGGTTTVYDESYGYEEPY
                     GYDGYVDVPVVYGEPMYYAPPVSVKFAFDYFTYEAIGGFVDVVFWRGGNRYHREPWYE
                     QGRRITDRDIRSGKRYHKVRGKEFYNHREKLRSKHNIVHPDSYYKLQRSKQKEQQRRL
                     EEQKKRELEMRKQREKQRLEEDRNRYNREKRELQEKQRRLEEQKKREL"
BASE COUNT         3557 a         3032 c         3571 g         3376 t
ORIGIN      
        1 aaaaaaacag ggggaaactt tacgatgaaa aagtggtgga cgcctgtctg aaactcttta
       61 gagagaaggg ctacaaactt atataaaagg aaaaaactga aatctcgggt atgatccttc
      121 tcgaggtttc atcctgtttt tttttgatga acatcgccgc cgctatttgt tttgcatttt
      181 gtccagtcgt tttttggcat ccgcgtggcc ctgctctgca gcttcgccgt accacacctc
      241 agccgaagca ggatcttttc tgacgcctct gccttcttcc agaatcacgc ctatcttgaa
      301 ctgcgcgtcg gcaatgccct gttccgctgc ctttaggaac catcgcgcgg cttcaacata
      361 atcctgtagc ccgctgctgt acatcaatcc cagcatatac tgggcatggg catgaccctg
      421 ctcagcagcc cgggtaaacc atttgatcgc cttcgggtaa tccaacgaga cgcctttgcc
      481 gttgttgtaa atacaggcca gattatactg ggcatcggca tgcccctgct cggcggccat
      541 cacaagccat ttaacagcct gagcataatc cagagatacc cctttttcat tctgatatat
      601 caagcccaga tgatactggg cgtcagcatc acctgtctcg gccaattttc tgaaacgctc
      661 aatatcttca ttcgtattca ttttgaaatt ccacaccttt tcatttgagg aaacattatc
      721 ataattgata gcggaaatct atacggatct gcttgatgat gcatgtgaaa atccccgggt
      781 cttccatgat tgatgaaaac ggtctctgtc gaaattatac gcataaccat tccttgacat
      841 tcaaacggta attccatata aataaaccta tcaataaaag ttttagcaaa ttcgattagc
      901 gtcatctttg ggggatcttt atgaatacaa aaattggtct atctcagatt ttattgtatt
      961 ttcttatgct gatgttcatg atggtcatag gttgcggcga cggggcggat gattttaaat
     1021 taccaaaata tcatgatgcc gatgcggaca aatgcaccat ttcaggcaga gttacctata
     1081 aaggtgtcgg gcgtgcgggt attaccgttc tcctgtcggg gtacagtaac gctactgtag
     1141 ctacagatga aaatggagaa taccgatttg acgacgtcga tggcgaccag gcctatgttg
     1201 tcagcccgag cagcggaacg ctgattttta cacccgaaag cagaggcgtg gaaccggacg
     1261 gatccaatgt tgacggtgtg gattttgagg cgatggattg ctgcggtgac gatacatccg
     1321 aggaagagga aacggaagaa atctgatcaa ccacggcaat tggtggatca tgccctcgct
     1381 tagtatcggc ggtttgcagg acatcggaat gtctttactt aaaatcataa atatcactgt
     1441 ataagtctcg atgagctgcc gtaaaatgac cgatgttgtt gcaggcgtgc tgttatggaa
     1501 cttcagcagt tccgtgagtt gttgttggac tttccagcgg gtatctactt cttatttctg
     1561 caaataatct gttgtgtttt tccttgagca tggtaaaact tgtctgcatc gttgaaccca
     1621 tgtacagaat tcttccgaca tcgtctccca gtccaagcga ttccagatcg ttcacatgcg
     1681 tcagattggc cgcaagaata tccgtgtcgg gattataggg cggcagcgtt ttaaaaatgt
     1741 cgccccgata ccttatcccg aattttgaca gacagacgat gcgtctgaca taatgttcat
     1801 tcctcatttt tgctatggcc tccttaagac ggcaggcaat ccgatagacg gacatccggt
     1861 tgatctccgc tcgggaaaag ccgataacgg cggagaaaac cgcgttgccg atgtacagcg
     1921 gatcaacatc gggatgaata tatcgcagat tgatggggat tctcaatctg atctgatccg
     1981 tgccgggcat gatgccgttg tgatactttt tcaataaaac ggcattgatg atttgattgt
     2041 tggacaggcg ataattctca ttttcggacg ttgccccgtt tttcatttcc gcaagcctct
     2101 tatctgaaaa gtattcacgt ctgtggtacc gcgtcgtctc ttgtgaattt tcgacccgct
     2161 tgcggattgt ttcatgcaag tcggaaaccg gcggggcgaa aacagaatca aaggtatcga
     2221 agacaacagg gctccccgaa aataatcgtt gcgtcgacgg tttggaaaaa ctgttgccgt
     2281 agtgaatgca cccccaggca taaagaaaaa gcatcagcga aacagcatcg ccaatggcat
     2341 gagaacaact gatcccgccc agcaatccgt ctcttaccgg gataatggtg acggcaaaca
     2401 aaggttctcc cggtaatgtt ttgacatgtc gcatcatgtg tcggacatca tcgatcgaga
     2461 tgtgagccgc atccgcatcc agcggcggta aaaccatata ttgaaaaccg tccgtacagt
     2521 acagaagcgc aaacctgttc tcatcaatca tgatcaggcg gctggaaaac agattgtaat
     2581 gtctgatcgt ttcaagaaaa ctttctcttg ctgccggaag cgatgttttc ttcttgtaaa
     2641 gaaccagaca ttcgattggg ggactgcccg gataatctgc attttcgaaa aaagacaagg
     2701 gcagtatatc gacaccgtaa tggtctttcg ggggaaacgt tgtggatgaa atatgtctta
     2761 tcatgacttg gatgccgggt aacgaatgct gtcaatcgct gcagattcgg tccctgccgt
     2821 tttgtttggc ctgatacatg aactggtcga cacgatgaac gaaacttttc atctcttctt
     2881 tcggtatata ttgcgaggca ccgatactca tcgtcacgaa aatttctttt ccctgcaggg
     2941 gggtaaagac ctctttcctg aattcatttt ggatcctctg ggccgtactc acggcatcct
     3001 cttttgttgt catgggcaga ataattgtaa attcctcgcc gccgtaacgg tacgccgaat
     3061 ctgaatcgcg caagcatttt ttgacgacct gcccgagtcg tgacagaaca ttatcgcctt
     3121 ccacgtggcc gtaggtgtcg ttaaatgtct tgaagtgatc aagatccaga agcaggagcg
     3181 acaatggatg atcgtagcgg gttgaccgtt ctatttcctt tcccagttcg gcgtgaaaat
     3241 gtctggaatt aaaaacctgt gtcaggtcgt caaggatgct cagctccaga tatcgctgtt
     3301 cgcttctctg cagagcttcc tccgccagtt tgcgttcggt gatatcccgg taattgataa
     3361 tgatcgcttc cacgacatga tctttgacga gattgcttcc cacggcttcc aatgtgcgat
     3421 aggtgccgtt tttgtgacgc agatgcattt cgccgtgaat cggcggcaaa tcgggttggg
     3481 tggccaaggt cgtaaacgat tggattaaaa attccaggtc atccggatga acaagttcga
     3541 atcctctgcg tccgattctt tcttctgtct tgtatcccag tacccgttca atggccgggt
     3601 tgatgtatgt gatcaatcct tccagattga ccagcacaat gatgtcggca gagtgctcca
     3661 caaaattccg gaatcgccgt tcttcaaact gtaatttttc ctccatcgcc ttgcgttcgg
     3721 aaatgtcccg ggcgatgccg atgattccaa cggcctcgcc gtttttatcc cttagaaagg
     3781 tcagggtggc ttccatccat ttctttgatc gatccttgaa gatatgctga tattccagcg
     3841 ttcgcgaccg ttttaaatcc cgtcccggct gtctgtccaa cgccagctct tccttcagaa
     3901 cttcggcaaa acggaaataa gtttccggtt cgaccatgtc ggaaatgttc gtccgggcga
     3961 tttcgtcctc gttataccct gaaatacggt agccggaagg actgaggtag gtgtatctca
     4021 gattcatatc catggtaaaa atgatgtcgg ttacgttgtc ggcaagcagg cgataacgtt
     4081 gctcgcttgc gatcagcaat tcctccgcaa acttgcgctc tgtaatatct ctgatgacgc
     4141 ccacggagta ttctttgcct tcgagggaga taagcgatcc gctgatatcg gcatataaga
     4201 gggtttgatc ttttttcagg atggggatgt ttctggcaat cgataattca tgatggttga
     4261 tcttattata aatgcttaaa atgtctttct gctgttcttt cggatgcaga tcggatattt
     4321 tcagcgcgag aaattcttcc tgggtatagc ccagcatttg gcaaagcgtt acattggcgg
     4381 caacgaaagt tccctgccgg gtatccagca tggtaatccc gtcagaagca gaatcaaaga
     4441 tcgttctgaa tttcaattcc atttctttcg cctgagaaag ggaatgcgtt accttctttt
     4501 tcagccgggt gatgtatccg cccataaaag aaaaccaggg aagaaccagc gccagaacga
     4561 tcagccctaa aatttcgttt tcggtattca gtgattcggg tcgatttttg taaagaagaa
     4621 acagaaccaa agcgtaattc aacacggcaa agacggataa gtaaagaaat tggcgcaaat
     4681 tcaacttgaa aagtccgaaa acgaaaacga ccagatagag aataaggaca gtgccccggg
     4741 catcatcggc gtaatacaaa ataaccattg cccagaacgt ggcgatgatc atctgaaaca
     4801 gtgtcaggct ggggtcgtca aatcgtttat tgaagccgga tcggattgca gcataaatca
     4861 gaatattgca taaaagaata cccatgaaac aaataaaaag aacatgaaac gataccggtg
     4921 caatgcccag cagataggtg atcagcgcaa tcgtcaacca cacggaataa gaagcaaagg
     4981 ccataagaaa tcgtttgatt cttacagact ggagaggatc gtcgggcgga aacagtttca
     5041 acatgaccaa tcgcctcaaa gtgatgaatt ttgtaattga ttttttttac cacaaaataa
     5101 aatcaagttc aatatatcgc tcgatgtatt tagaaagaac gatggatgcg cctgacgcct
     5161 tcaggtaaaa tatacggtaa aattttcgta tctgtgctat taaaaaaagg gtaacagttc
     5221 gtacaacact tgacatcaac tcgggggtca tcatcatgtc tcataaattg aaaaaagaaa
     5281 gtgatgatgc cggtatcgtc attacttttt ccggcannnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnntccg acatgttcag ttttaaatcc ccgagtttta aggcgctggg
     5461 attgaatcct gccgggctcc acgataagga cctgatgaat ctgatgctga gtgagccgcg
     5521 actgatccgc cgtcccgtcg tgaaaatagg aaggcatgtt tattttggcg cggacatcaa
     5581 ggtgctgagc gaaattatca ctcagccggt ggaaaacggt tgatcatttc tggtgattgc
     5641 aagtgccgaa gaagacgagc gcagggatag ttttatatcg atttgtggga gaggatatgg
     5701 aagtttttct tgttcatccc ggaggtcctt tctgggcgaa aaaagacaag ggggcgtggt
     5761 ctattcccaa agggctgttt gaaccggggg aagatccgct ggaggccgcc cgacgggaat
     5821 ttcatgaaga aaccgggcat gcggtgtccg gcgagtttag ggcgttgatg cctttgaaac
     5881 agcccagcgg gaaagtggtt catgcctggg cggtcgaagg acatatcgat gaaaaatcca
     5941 taaaaagcaa tatgttttcg atggaatggc cgccccgttc gggaaaagag cagtccttcc
     6001 cggaagtgga ccgtggcgga tggttcacac tacgcattgc ctgtgaaaaa atattgccgg
     6061 gtcagcgcgg tttccttgaa gagcttcaaa caaagattaa aaatcgtccc gcctgagtga
     6121 tgtctggcag cgaccatgtg gcggaaaatg aattgactca tgaataaaat atctttacct
     6181 acacaaacga aaaagacgtc atcttctgcc cgggtgcatc agactggggc tttgttgtaa
     6241 aggaaattat tatggatcag aagataatct gtctcagcgt ggggttgtcg cagaaagaga
     6301 ttgaaaaagg caaggcgttg tattttgccg ccagcgaagg ccaatcgacg ctggaggtga
     6361 ccgccatgtc ccggtccatg ctgaatatga aaatcattga aatactggca tgcctgatgg
     6421 aggagctgga aaagaatccc gcagagaaac aaaacgattc cgcggacgtt attccggcgg
     6481 atcattgtaa ggccgcagtg cttgtcatgc ttgcgcagga tcgtgagctg gtgttgcagg
     6541 tgatgaggag ttttaaagcg gtattgtctg attcccggaa tgtgatcttt gcggtcataa
     6601 cggagacggc gctgaaatgg acgctggcgg aatatcttga acatctcagc cgggaacatg
     6661 aatatatgca gaccagcagg ccagaaaata atccggatat gaaaaagttg taaagtgttt
     6721 tgcaggagaa agaaacgatg atttacaatg aagaatacga aacgctgccg cgcgaagcgc
     6781 tggaggcatt gcaattaaag cggttacagc aggttgtgaa ccgggtgtat catcatgtcg
     6841 gtttttacaa aaaatcgttt gacagggcag gcgtgcaccc caatgatatc cgcacgcttg
     6901 atgatctgaa aagatttcct tttaccacca aacaggatct gcgcgacaat tatcctttcg
     6961 gcatgtttgc cacgccgatg agcaatatcg tccgtctgca tgcttcatcg ggaacgacgg
     7021 gcagttccac cgttgtcgcc tacacgaagc gcgatatcga aaactgggcg gagctggtgg
     7081 cgcgctgttt tgcagcagcc gatctgaccc ggggcgatat cattcataac gcttacggct
     7141 acggtctttt cacaggcgga ctcggcgttc actacggtgc ggaaaaactc ggcgccagcg
     7201 tgattccgat gtctggcggc aataccaagt ggcagctgat gattctgcag gatttcggtc
     7261 cgacggcgat ctgctgtaca ccttcctacg cgctgaatct ggcggagcag ggccgggcga
     7321 tgggaatcga tatgccttcg ctgaaattgc gtgtgggcat tttcggcgca gaaccgtgga
     7381 gcgaacagat gcgcgcccag atcgaggagg cgtttgccat caaggccatg aatatttacg
     7441 ggctatccga aatcatgggg cccggtgtat cgatggaatg cagcgtggcg caggccggta
     7501 tgcatgtttt tgaagattat ttcctggtgg aaaccatcaa accggatacg ggggaggttc
     7561 tgcctccggg cgaacaggga gaactggttt ttaccacact gaccaaggag gcgttcccgc
     7621 tgatccgtta ccggacgcgc gatatttccc gcctgatcac gacgccctgc cgctgcggcc
     7681 ggacgtttca ccgcatggaa cgcgtcatgg ggagaagcga tgacatgctc atcatccgcg
     7741 gcgtcaatgt ttatccgtct cagatcgaat ccgtgctgat caacattgaa gggttggagc
     7801 cccactatca gctgattatt gaacgttcag gcacgctcga tacgcttgaa gtgcaggtgg
     7861 aagtgacgga aggggtgttt gccgaggccg gcgaagtgaa gggattgcag caactggaga
     7921 aacgtctgat caaggatatg aaggactatc tgggcatcac ggccaaggtc aaactggtcg
     7981 aaccgaaaag tatcatgcgt tcggagggca aggccagccg ggtgattgat aaacggaaaa
     8041 tttaaaacag tcgtaggaca ttccattata ttatataatg ctgttactgg agggaaacgt
     8101 tatgaagccg atcaaaatca tgccgtgtct ggatatgaaa aacgggcggg ttgtcaaagg
     8161 agttcatttt gtcgatatca gggaagcggg cgatccggtg gaaaatgccg aattttatca
     8221 ggccgaaggc gccgatgaac tggccatgct ggatattgca gcgactctgg aaaaccgtaa
     8281 aacacgtctg gaatgggtga aaaatgtttc gaaagtgatc aggattcctc tgaccgtcgg
     8341 cggcggcatt gcttcgctgg aggatatcga catggtgttg ggtgcgggcg ccagcaaggt
     8401 ttcgatgaac agcgcggcgg taaaaaatcc ggaattgatc cgtcaggcgg cggaaaaatt
     8461 cggcaaggaa aaaattacag tggcgattga cgcgaaaaga aacgctcaga tgccgtccgg
     8521 ttttgaactg gtggtttccg gcggcacgat gccgatcgcc cgcgatgccg tggcctgggc
     8581 gaaaatctgt caggaaaaag gtgccggcgt gattctgcca accagcatgg acggcgacgg
     8641 caccaaagcc ggttacgaca tccctttcac cagggcgatt gccgatgcgg tgacgttgcc
     8701 cgtggtggct tccggcgggg cgggcaaact ggaagatttc tacgaagcgg tggtccaggg
     8761 cggtgcgacg gtcctgctgg cggcctcggt gttccatttc cgtctgctca agattcagga
     8821 tgtgaaaacg tatctgcggg aaaagggcct ggccgtcgcc tgatgagaaa tgtcatgagc
     8881 aaaaaaaata acgccgggtt catgtatctt gctttgcttg gggcagctat aacgacgctg
     8941 tgtgacgggg tgcatgttta tacgcaaacg ctttcttata cgaccccctt tcttttcggt
     9001 caggcctggt gggtttttcc gggatttgtc ttggccttcc tttttatggg cggggtctat
     9061 tttgggctag tgaatcgtct cccgttattt tttgctgtga agaaaagcat atccggggga
     9121 agtcttcgtg atgtcgttga aacggccacg gcctttgccc tgatttatgt gatgagcgga
     9181 ctgggcagca atgaaccgct tcttctatcg gcggttttct acggaacctt tgttttgcgg
     9241 ctggcgttca gttatgaccg gctatttctg cttttactcg cggttctgat ggcctttggc
     9301 gggatgtttg tcgaaagctt gtatgccgct gccggattga tggcgtatcg ttatccggat
     9361 gtgttttatg tgccgttctg gctgggcgga ctgtatgtac acggtgcttt tgccctgcgg
     9421 gagggcatgc gattttttgt ctatgacaga aagccgggga cgttaatttc cgtctcccct
     9481 ttatgatgac agcgtttcat gcagtgtcat tcggtgcatc cggccggtgg cctccgatga
     9541 tggtacatca tgatgttgtt aaatccggcg gtgacgatca gctgacaatg agataaaaca
     9601 tagtggtgaa gatgcgtgct ggttagaaaa cgatgatgac cgaagataaa aaaacgtctt
     9661 ggcgaaataa gaaaagcgat ctgcgcaggc tcgttcttct gatgaacaga cagaataaca
     9721 gattctggcc gccgctggat ccgattctca aaacggttga tctggtaatt gagccggaag
     9781 aattgaatct cctgctgaaa atggggaccg atttatacac ttatgatcag gttgccgcgc
     9841 tcagcggcat aaacagtgaa aaattcgacg ccgtcttcga ctccctgaaa aagaaagcgt
     9901 tcatcggaat aaaaaatcct gaaacgtctg gcgagcagta taccctgcat ccgttcgtgg
     9961 tcggctggtt tgaaggccag gtgtcttacc tgatcggcaa acccgaagaa aaagaatttg
    10021 ccaaacggta catgggcttt ctggattctc tgcgcaaata caattttttc cccctccgga
    10081 atatcatgaa tgtcatcggc aaacacgcga ccgtatccaa tcaaagcgtc ggaacggtgc
    10141 gcgagtccag aaacggtcag ggaaaatcca aaattgtcgt ggatgaaaac attgtcgtac
    10201 cggattccgg aatttatccg acaagctcgg taaatgatct gatcatggaa ttcggaacca
    10261 gaagtattat cggtcagttt aaggtttgca tgtgccggca gatgaccgcg aatgtcgatc
    10321 aaccctgtcg cctgaaaatg cccgatgatg ttgcctgtat gggatttggc gagggcatca
    10381 aaacctacat gaaatacgga tatatgcgcc ggatcagtaa agagcaggcc tttgatgtca
    10441 ttcaaaaagc cagggatcac ggcgccgttc ataccgtgtt tcatgaaatg gatgatacaa
    10501 aactgccgca gattggttta tgtaactgct gctgggactg ctgcggtatt ttccgtacgt
    10561 ataatatggg cgttgtccct ttgcgttacc ggtgttatta tatagcaaga atcgcaaaca
    10621 gcgaccgatg cacgggctgc ggccgatgtg aaaagttctg tccgacatcc gcaatcaacg
    10681 tggtggcaaa aagcgtggtc ctcgatacaa aaaaatgcat cggatgcggt cagtgcgttc
    10741 atcaatgcgc tcgtgcggtg gttgaactcg ttgaggacag acgtacggcc tttttaccca
    10801 tgctgaaaac atcggaagcc aggctgctgt aggaaaaagt tgcgattcaa tctcggaaac
    10861 ttcagaaagg atgcgatatg ccgggtttaa agaaaaagca acacctgccg gatgatttga
    10921 aaaaatgcgt ggaatttcac gggcacatct gtcccggcct gatctacggt tatcgggtgg
    10981 cccaagaagc gatacgactg atgcatatcg gacgctcggt ggatgaggaa gttgtggccg
    11041 tctgtgaaaa cgattcctgt gccgtggatg cccttcaggt gatgctggga acggtggcgg
    11101 gaaagggcaa tctgttgatc aaaaattacg gaaagaacgc ctttacggtg atgagccgag
    11161 ttaatcgcaa agcttaccgg ttttcccgca aaaaacaata tgcgtatggc ggctcttccg
    11221 gacgccggtt taaaaagctg gaaagggctg ttgcatccgg agacgcatcg gaagaggaga
    11281 aacaggaact gaaacgtctg aaaatcgacg atctgctggc gcgtcccttt tcggacatct
    11341 ttaccacgga agaagtcgtt ttcgacgaac ccggttatgc gccgctggcg ccttcggagc
    11401 cctgtgccgt ttgcggtgaa atgaccatgg caaccaaaat gatcccgttg cgcgacggaa
    11461 ggcaagtttg cattccctgt tcggagaaaa gtaaacattg acatattcgg gaagtatcgc
    11521 taccgtcctt tgaaacagat aaatcaattc aaggagattt cctcatgaaa aaaataggca
    11581 ttacaattct ggttgtgctg ggagtcttaa tgacgtcgat tgcatacgcg gccaatctct
    11641 acggcatcgt gtccgataaa aacgggaagc ccgttgaggt aaaagtgacg ctcaaggacg
    11701 acaaaggcgc tgccgtgggt cagcctgtga cgacggataa aaaaggagct tatgcgttta
    11761 aggatatcaa gcccggttcg tatgtagtgg tcgtcggcgc taaggacgaa tggaaaatat
    11821 tcgtgggccc cggcgaaacg cggcgcgatt tcagtttgaa ataatatcca cggtgaggtt
    11881 ttggagaatg gctgaacgcg acatccagaa acaagaagaa ccgacggagg ctcccaggaa
    11941 gaaggacctc tgggatatcc tgcaggcact gggaccggcg ctgattgcgg ctgccgtagc
    12001 tgtcatcggc tcggtgtaca gctatcagca ggcccagatg accaaggctt ccgaaatgcg
    12061 ggaggtctat accaagatca tgacggagcg cgaaaattcg gacaataata ttcgcgccag
    12121 tatgttccag atgcttttca atgcgatgtt cgacaaggat atcaattccg cggacacgcc
    12181 ggataatgtc aaccagatca aaaaacgagt gatgtttctg gatgtgctgt cacgcaattt
    12241 cgatactgtg gacatcaaac ctcttttcga agatctggat ggagagttga ccagaaaaat
    12301 gtataatgac cgatcgtatt ccgacggcgc ccaagaagac tattttgccc tgcggtccga
    12361 attgcggcgc atcggtagaa atcttgccgt caaacagctc aatgctctgg catccttacc
    12421 cggcacgatg gtcaaaacca tcgttctgat tgaagataag gataaaaata tcatggtgat
    12481 gcaggatgaa actacggcga acggaaacgg tgatacgctc cccgtggaaa tcaaagcgca
    12541 aaatcttgat gacggcaagg tggaaatcgc catagattat cagaaagcca aagcttccga
    12601 taaggggttc aaggcgcctt cctttattat taccttctat gacatgcctt atatcgacaa
    12661 cagcgtgctc gacaaggata tgcgcgtggg cgtggtgctg accaaatatg ccacgacgag
    12721 ggaaatcgat ttgttccgcg accggatcga ccggcgtctc ctcaaagatt acgaagacct
    12781 caaagccggc ggaattgcca aatatgccga actgcggttg atcaaatttc cggcgaaata
    12841 caccggccac cgcgatcgac cttatctgca ggagattctc cagaatctcg tcgaaaatga
    12901 gaaatccgtc agataaaccg ggcgatcaat gatgtcggat gaaagaaaga aacgatatac
    12961 aacaaacaag tgtttccaga tcccgggatc tgatgaaagg aggataatgt ttatgaaaaa
    13021 atatgtgcca gtcttgttgt tgctgatttt tgtttttgcc tgctgggcct gtgttcccgt
    13081 gcccgttccg gtaggcggaa cgacaacagt ttatgacgaa tcgtacggat atgaagagcc
    13141 gtatgggtat gatgggtatg tagacgttcc ggtcgtttat ggagaaccga tgtactatgc
    13201 gcctccggta tccgtcaagt ttgccttcga ttactttacg tatgaagcca tcggcggatt
    13261 tgtggatgtt gtgttttgga gaggcggcaa ccgctatcac cgggagccgt ggtatgaaca
    13321 aggaagaaga attacagaca gagatatacg ctccggcaaa cgatatcaca aagttcgcgg
    13381 aaaagaattt tacaatcatc gcgagaaact gcgcagcaag cacaacattg tccatcccga
    13441 ttcgtattac aaactgcagc gctccaaaca aaaagagcag cagcgccgtc tggaggagca
    13501 gaagaagcgg gagctcgaaa tgcgcaagca aagagaaaaa cagcgattgg aagaagatcg
    13561 caacaggtat aacagggaaa aacgcgagtt gcaggaaaag cagcgccgcc tggaggaaca
    13621 aaagaagcgg gaactg
//
LOCUS       DAOSME010000059        13626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_5269_length_13626_cov_18.752855, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000059 DAOSME010000000
VERSION     DAOSME010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5269_length_13626_cov_18.752855"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1581)
                     /locus_tag="PLG98_08725"
     CDS             complement(205..1581)
                     /locus_tag="PLG98_08725"
                     /inference="COORDINATES: protein motif:HMM:NF010635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOE33381.1"
                     /translation="MKINEAECLEKWSSMAASSSLVDSSLYTKYDVKRGLRDIKGKGV
                     LAGLTQIGEVQSHTQIGDDESELGPGCLIYRGIDITEIVSGFVADKRSGFEESCYLLL
                     FGELPDKEELERFQKLLISYRSLPDDFIHDSMLKLSSRDIMNAISQSILSMYVLDDRA
                     EDIEIPNVLRQCIKLMAVFPLLLVYCYQAYAYRYGNQSLVIHNPGRQLSTAANFLHML
                     RHDSKFTALEQKLLDLALVLHAEHGGGNNSSFTVHVVTSSGTDTYSAIAAAMGSLKGP
                     RHGGANIKVVRMFEDLKNNISNWNDDRQIEDYLIKLLNKQAFDRAGLIYGIGHAVYSV
                     SDPRTLILREYVGKLAQEKGLSEEMKLYERVERIAPEIIGHERKIYKGVSANVDFYSG
                     FLYRLLDIPPELYTPLFAMARIAGWSAHRIEELSNGGKIIRPAYKSVTPRRHYVPMKD
                     RNSNEQNR"
     gene            complement(1892..2485)
                     /locus_tag="PLG98_08730"
     CDS             complement(1892..2485)
                     /locus_tag="PLG98_08730"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOE33382.1"
                     /translation="MGKTFLIFLLAYLAGSVNFAIIFFKLTGRKDPRLGFSGNAGTTN
                     VYRQAGMVWAAVIFLLDIGRAMLVAVLAMYYLEPAWLPWAGYFLVLGNSYPCFHEFRG
                     GKGVANYLGFTILVSPWAAMLSCAVWLLVYGLVRMPFIASFFMVFTLACGQAYHSHWN
                     WGAIAGSLMTWILILYNHRINIMAHRTGEKDVPHENK"
     gene            complement(2587..3450)
                     /locus_tag="PLG98_08735"
     CDS             complement(2587..3450)
                     /locus_tag="PLG98_08735"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HOE33383.1"
                     /translation="MENISGLNERQRYPFDLEIYPYLRDHHLEKRAILPAVESLIILA
                     GACRAVSLQRGIKCIRKAAFSRFLYLAPDNRRQPAVIDIIKTEGGNMVASLLTSVQSK
                     TGTISRHVEHARAECVISGFEETGPPSFRSVDKLKGSCISIPSATVYRDLVPFGIAYQ
                     NIIGDLSVSSEGALGYVSGGNFEADDELLGSPFPLDAVMHTACVWGQRFAGKVCFPVG
                     FEKRLIYQKTKKGQEYLGRVVPVSITRESLIFDAWIYKDDVLYESVSGIIMKDVTNGR
                     MNPPDWIKVNS"
     gene            complement(3429..3908)
                     /locus_tag="PLG98_08740"
     CDS             complement(3429..3908)
                     /locus_tag="PLG98_08740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE33384.1"
                     /translation="MKPKPFKPEIYHNDERYVRDLTTGLVWHRSVYRVLYIDTDRSQV
                     VYHSNYLRYFEFGRTSLMRDLAYPYKEIEDGGYVYPIFDLGISFHQPLYYDDVMYIHT
                     RPVNLERVRLQFDYLITHQEKGNIICAGFTKHCALNLSGTPVAVDPKTVHLWKTFPV"
     gene            complement(3910..5175)
                     /locus_tag="PLG98_08745"
     CDS             complement(3910..5175)
                     /locus_tag="PLG98_08745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOE33385.1"
                     /translation="MRAPKNRRVFVVGYGAATPLGSTFEKTWKRAVKGEAGFRKVTRC
                     KVESACDVVGEIPDWYPMELDFTDAKEVYNWNAAFVILTMAVCKEALENAGIIVDAQI
                     APRMACLIGSALNGSDAFRIAVHDLDHRGPLRVSPYLLPNLCANLPSGKAGMLLKFTG
                     PIFSPQGACASGNHAIGIGARMIRDGDCDFVLAGGADTPILPELIHGFANMNATIKVN
                     PKDRAYDNPALASRPFSADRKGFVLSEGAGVVVLAADEAIKTYGLKPKAEVLGIGWTS
                     DAHHYTSPNMPTIIRAIRETIDDAELEPTDIQYINAHGTSTPKGDTTEIKCLREVFGK
                     KIEKIPVSSNKSQLGHTLGATAAIEAALTIEGMKKGIILPTINHLPDPEFSDIDVVPN
                     EARKNKCDIALSNAFGFGGTNCCVIFRGV"
     gene            complement(5268..6518)
                     /locus_tag="PLG98_08750"
     CDS             complement(5268..6518)
                     /locus_tag="PLG98_08750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOE33386.1"
                     /translation="MGLKKTVIVGYDAVSSLGAQLDAQWRQAVAGQSGIGRLTRFPLN
                     ENFPVRVAGEVEEIDPGPFPFLQPREMAHWTSPIFKYALLVVHRALQNGGIEITRDIA
                     PRVAITFSSAVGGLDAVLQADRLMVKDGKLPHPFTNPNSCINMVGGKVSILTGATGPI
                     CSTITACATGITSMIIGEMLLQRNMADVVIAGAVDFPLVEPIVAGFATMNGAYRPKEG
                     QPEEPPEKTSRPFSANRRGFVVSEGAGCIILATGEFVKVHGLPSGMEIAGWAMTSDAS
                     HFVSPNLATVQKCMEETIANAGLKPEDIDAVNAHATSTKIGDKVEADALKNIFGGHVP
                     PVSANKSQMGHAMGASSAIEAILAMEGMKKEIVLPTINYRPDQDIAIDCVAEGARNLK
                     QEFVLKNAFGFGGCNSCIILHRTE"
     gene            complement(6604..>7491)
                     /locus_tag="PLG98_08755"
     CDS             complement(6604..>7491)
                     /locus_tag="PLG98_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33387.1"
                     /translation="RCKGCFFFSSGEHQAAVEEMDIGKWEAFIDREKERGVNLAILIG
                     GEPTLCLDRVEAFYKRLPTYCATNGLIKVPRDRFPDMMVGISLWGSAEDEKILRGRDT
                     FAVSSKNYEGDAYTYYLYTITPRQVGKIEPVIKRIADVGLKVHLQLLSNDEGVDGFSW
                     QEGELKDLRLEMDEMLDTYSQTVISCKYYHEIITTGKMMGRHFGWSECPSVTEALDNR
                     ANRPRRLIHFARWASDLKTVHRCCTSATRDCSTCKDGAAHMSWVMVNKREHLKSTKDL
                     QNWIEVYEMFAKLYHFIPW"
     assembly_gap    7492..7591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7920..9005)
                     /locus_tag="PLG98_08760"
     CDS             complement(7920..9005)
                     /locus_tag="PLG98_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33388.1"
                     /translation="MANQVNNKVNTESVKPKFSELLARPEVKARWEKVRKYFFLREST
                     YDMTNRCNIRCDGCYYYEGEKQFAKEVGDVEAWRALMRAEKARGITYVVLAGAEPSLV
                     PELLEVCYQEMPLGSIATNGFRKIPPSVGYKLHISVWGSDETSLKIRKAKNLLKKQIE
                     NYENDPRAVFVYTFTRNNIDEVNEVTEELAAHDCKLTFNVFSAPVGYAGNLRHDDASL
                     EQTRQTMIELLNQYPRNVLFSVYNAIAHTHRKGLHDLYSCSYPRQNPSQDIGLGRSFR
                     QYRTDLNWDRSVACCVPDTDCADCRHYAAGSAVVTARLYRHVTNLATFKSWLDYVDTY
                     LAVWVMNYEKGENLCNEIIEPPRATVS"
     gene            complement(9021..9293)
                     /locus_tag="PLG98_08765"
     CDS             complement(9021..9293)
                     /locus_tag="PLG98_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOE33389.1"
                     /translation="MPASQDVKGIIRRVAEIIIDELKLEEATADTFDPDMDLVDELGI
                     DSMDLATIALVLQDEYRITIDEDDYPQLKTIRLISEYIKERLPVRH"
     gene            complement(9333..9704)
                     /locus_tag="PLG98_08770"
     CDS             complement(9333..9704)
                     /locus_tag="PLG98_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33390.1"
                     /translation="MKNQWNLLKETREADSNDIEALAEVPADSPWFSGHFPGEPILPG
                     IALVYAVKQAIHREAQKKGERLELHTLKRIRFMQPVRPGDQLSLNIRAAEADGERLFH
                     FKIFCRDTMVSSGMIAAKKMM"
     gene            complement(9832..10632)
                     /locus_tag="PLG98_08775"
     CDS             complement(9832..10632)
                     /locus_tag="PLG98_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOE33391.1"
                     /translation="MKKKLSSANPAEMTGVILLLAAAVIFFFHPLAAAAVALFYIVLC
                     VAASFFPQSSFYLPVISRGRTGRNVVALTFDDGPAEPTTRRILDLLDRYSVKATFFVS
                     GVNALKHPDIVREIIRRGHTVGNHSFHHNPFLMLGSYRYLYNEVSKAQDILKDRGVNT
                     LIFRPPVGIINPKLPSVLDKLGMVCVTFSCRGGDFGNRRIKNLSSRILHSVKADDILL
                     LHDALPRGGGNQAVLLIEIEKILRGLKKQGLNVVPLADLIGRHVSIMM"
     gene            complement(10838..12190)
                     /locus_tag="PLG98_08780"
     CDS             complement(10838..12190)
                     /locus_tag="PLG98_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HOE33392.1"
                     /translation="MMNVLLISANTMKNPYPVYPLGLDYVAGCLDSRYRTKIVDLNVF
                     SHPETLETMIREFSPDVVGISIRNIDNTDAINCRSFLPEYETLVGQIRKNSRAKIILG
                     GSGFTIFPREFMQVLDADYGIVGEGERLALLLDAWEKHEDVNRIPGVLTRAMTDIVYE
                     PFGGRLNRRFDPEETHTRFYLAYGGMLNIQTKRGCPFRCSYCTYPHIEGGRMRCCDPE
                     EIARSARALQDAGAKYIFITDSAFNASYEHSGQVAKAFMKAGVSIPWGGFFASTVPPA
                     DYFKRLADAGLTHVEFGTESLSDPVLENLHKPFTSENVFTAHREALDAGLYVAHYFLL
                     GGPGENKETLQATLMGIDKLERAVFFFFCGIRIYPHTALYDVAVREGQIKAGENILPP
                     VFYRSQGIGVEEIMKTVENHANGRLNWLIGAGEAKATRILPKLYRRGFTGPLWEYLIQ
                     "
     gene            complement(12234..12506)
                     /locus_tag="PLG98_08785"
     CDS             complement(12234..12506)
                     /locus_tag="PLG98_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOE33393.1"
                     /translation="MSNAAEMIRELKIKIIETLGLIDVGPDDIQDDERLVGGDLGIDS
                     IDVLELVMMIEKDYGVKIDSKKLGVKVFASVSALADHIINHQKKTV"
     gene            complement(12499..12915)
                     /locus_tag="PLG98_08790"
     CDS             complement(12499..12915)
                     /locus_tag="PLG98_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33394.1"
                     /translation="MNIVKKTAVYFARKEFCRNAINECADLSLLKEKHTLRVTIGIVL
                     IVLSYIVVLPSFFMVGVMAARLRKPMVGVIGIPLAYGFSWLIMMMGMYLTGPEYAKTL
                     GKYVVRVVLEKILGDEAKKIASKPNKNIDKTGEPNE"
     gene            complement(13001..>13626)
                     /gene="fabA"
                     /locus_tag="PLG98_08795"
     CDS             complement(13001..>13626)
                     /gene="fabA"
                     /locus_tag="PLG98_08795"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF003509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HOE33395.1"
                     /translation="VVDLSARRETIMKYQEFLKRDHFNFEEILAFAYGKLVDDPPEHF
                     DAKFPAPPFLMVDRILLIESDGKKGKIIAEQDIRPDAWYFQCHFKGDPVQPGCLGVDG
                     LWQLLGFFCVWRGSLGTGRALGCGDVAFNGQIRPFNKCVRFEVDVRRYSMLKESGASI
                     VIGDGHLYVDNELIYTVGQARVGVFKDIAYKDYPLRSKYSIGGIMER"
BASE COUNT         3446 a         3548 c         3086 g         3446 t
ORIGIN      
        1 tcctacgccg tggaaaccta cggcacgcag gaatacagct acacgtccga agaatatcat
       61 caaagactaa acgatttatt ataaccgcag acaacactcc tgacattccg gaggcgtgct
      121 caataaaaaa tcattctcca attttttttt gcggcgtcat tagaaaaaat ccgctgatga
      181 tcaacggctg ttttcacggc cgggctatct gttctgctcg ttgctgtttc gatccttcat
      241 aggcacatag tgtcggcgcg gcgttacgct tttataggcg ggcctgatga tttttcctcc
      301 gttggacagc tcttctatcc ggtgcgcact ccagccggca attctggcca tcgcgaaaag
      361 cggcgtgtac aattcaggtg gaatatccag gagacgataa agaaaccccg aataaaaatc
      421 gacgttggca ctgacgccct tgtatatttt tcgctcatgt ccgatgattt caggggctat
      481 cctctcgact ctctcataga gtttcatttc ttcggagagt cccttctcct gcgcgagttt
      541 tccgacatat tcccttaaaa tcaacgtgcg cggatcggaa accgaataaa cagcatgacc
      601 gataccgtaa atcagaccgg cccggtcaaa agcctgctta ttgagaagtt tgatcagata
      661 atcttcgatc tggcggtcat cattccagtt ggaaatatta tttttcaggt cctcaaacat
      721 tcgcacaact ttgatattgg ccccgccgtg acgcggaccc ttgagcgacc ccatggctgc
      781 ggcaatggcg gaataggtat ccgtaccgct ggacgtaacc acatgcaccg tgaaagagga
      841 attattaccg ccgccgtgtt ccgcgtgaag caccagtgcc aaatccagca gcttttgctc
      901 cagagccgtg aatttgctgt catgcctgag catatgcagg aaatttgcgg cggttgataa
      961 ctgccgtccg ggattgtgaa tcaccaggct ttgattgccg taacgatagg cgtatgcctg
     1021 ataacaatac accaggagca acgggaacac cgccatcagc ttaatgcatt gccggaggac
     1081 attcggaatc tcgatatcct cggcccgatc gtccaaaaca tacatggaca agatactctg
     1141 ggaaatggca ttcatgatat cgcggctgga aagtttgagc atggaatcgt ggataaaatc
     1201 atccggtaaa gaacgataag aaatcagcag tttttgaaac cgttccagtt cctccttatc
     1261 gggcagttca ccgaaaagca gcagataaca ggattcctca aagccactgc gtttgtccgc
     1321 gacaaacccg gatacaattt cggttatgtc gatccctcgg taaataaggc accccggtcc
     1381 gagttcagat tcatcatcgc cgatctgcgt atgagactgc acctcaccga tttgcgtcag
     1441 cccggccagc acccccttgc ctttgatgtc gcgcaatcct cttttaacat catatttggt
     1501 atacaaggaa gaatccacca ggctgctgga tgccgccatt gagctccatt tctcaaggca
     1561 ttccgcttca tttattttca tatgtttctc cttccctgtt ttgtgtattt cgccgaatat
     1621 tcagacaaaa taaaatagta ttctgcagat cggtatttta caatacctta taattcaagg
     1681 acaaaagtgc ttttttgacg ggcaacattg aatgaaagaa tgtagcctct gaagtgtgct
     1741 ttctaatatt gcgctgtgcc caaatgacgt ttttaatata gtcaatgaca gcggttacgt
     1801 caattatttt tattttgaac attgtcagcg tataaaaacg gttggttctt aaagtagaac
     1861 tttaagtctt aaatactatt tcatttcacc gttatttatt ttcatgtggc acatcttttt
     1921 ctcccgtgcg atgcgccatg atatttattc tatggttgta cagaatgagt atccatgtca
     1981 ttaaagaacc tgcgatcgcg ccccaattcc aatgggaatg ataagcctgt ccgcaggcga
     2041 gagtaaaaac cataaaaaaa gaagcaataa aaggcatccg gactagtccg taaacaagca
     2101 gccagacagc gcaggaaagc atcgctgccc acggtgaaac aagaatcgta aatcccagat
     2161 agttggccac cccttttccg ccgcgaaatt catgaaaaca cggatagctg ttgccgagga
     2221 caagaaaata accggcccag gggagccatg cgggttccaa ataatacatg gctagcaccg
     2281 cgaccagcat cgcccggccg atgtcgagca gaaaaataac ggccgcccac accatgcctg
     2341 cctgacggta aacgtttgtg gttcccgcat tgccgctgaa cccgagacgg ggatcctttc
     2401 tgccggtcag tttaaaaaaa ataatagcaa aattaaccga tccggccaga tacgctaaaa
     2461 gaaatattaa aaatgtttta cccataatga gactcgctga gaacaagcaa aacgaagttt
     2521 gaaacgctgt cgaatgatcc caggcacgcc gtgccgcggg atacccgtca attttaccct
     2581 tcgctctcaa ctattaacct tgatccaatc cggcgggttc atccttccat tcgttacatc
     2641 tttcattata atgccgctga cgctctcata gaggacgtcg tccttgtaaa tccaggcatc
     2701 aaagatcagc gattccctgg ttatgctgac cggaacaaca cggccgagat attcctgccc
     2761 ttttttcgtt ttttgataaa tgagtctttt ttcaaatcca accgggaagc aaactttgcc
     2821 ggcaaagcgc tgcccccaga cgcaggcagt atgcatgaca gcatccagag gaaaagggga
     2881 tccgagcaat tcatcgtcag cctcaaaatt tccacctgaa acgtatccca gagcgccttc
     2941 ggaagagacc gacaaatcgc cgatgatgtt ttgataggca atgccgaaag gaaccagatc
     3001 gcgatagacg gtagcggacg gaatgctgat acagctgccc ttcagtttat ccaccgagcg
     3061 aaaagaaggc ggacctgttt cttcaaaacc cgagatcaca cattccgctc ttgcgtgttc
     3121 gacatgacgg ctgattgtgc ctgtttttga ctggacggac gtcagaagcg acgcaaccat
     3181 gttgccgccc tccgttttta ttatatcgat aaccgccggc tggcgacggt tatccggcgc
     3241 caggtaaaga aatcttgaaa aagcggcttt ccgaatgcat tttatcccgc gctgcaaaga
     3301 aacagcccgg cacgctccgg ccaaaataat cagcgattcc accgcaggga ggatcgctct
     3361 tttttccagg tgatgatccc gcagatatgg atatatctcg agatcaaacg gataccgctg
     3421 gcgttcattt aaaccggaaa tgttttccat agatgaaccg ttttcggatc cacagccacg
     3481 ggcgtgcctg acagattcaa ggcgcaatgt ttagtaaaac cggcgcagat gatgtttcct
     3541 ttttcctgat gcgtaatcag atagtcgaac tgcagacgca cccgctccag attgaccggc
     3601 cgtgtatgga tatacatgac gtcatcataa taaagaggct gatgaaagct gatgccgaga
     3661 tcgaaaatgg gataaacata gccgccgtct tcgatctcct tgtacggata ggcaagatcg
     3721 cgcatcagcg acgtgcggcc gaattcaaaa taacgcagat agttggaatg ataaaccacc
     3781 tgcgagcgat ccgtatcgat atacaaaacc ctgtacactg aacgatgcca gacaagaccg
     3841 gttgtcagat cgcgcacgta gcgttcatcg ttatggtata tttcaggctt gaaaggtttg
     3901 ggtttcatct tagactcctc tgaaaatgac acagcagttt gttccgccaa atccgaatgc
     3961 gttggaaaga gcaatgtcac atttattttt tcgggcctca ttgggtacga catcaatgtc
     4021 ggaaaattcc ggatcgggca ggtgattgat ggtcggtaaa ataattcctt ttttcattcc
     4081 ttcaatcgtc agggccgctt caatggccgc ggttgctccc agcgtatgcc ccagttgcga
     4141 tttatttgac gatacgggaa ttttttcgat ttttttgccg aacacctcgc gcagacattt
     4201 tatttccgtg gtgtctccct tcggtgtcga ggttccgtgg gcgttgatat attgaatatc
     4261 tgtcggctcc agttccgcat cgtcaatcgt ttcccggatg gcacggatga ttgtcggcat
     4321 gttggggctg gtgtaatgat gagcatccga cgtccagccg atgcccagaa cttcggcttt
     4381 cggtttgagg ccataggttt tgatcgcctc atccgccgcc agaacaacaa cgccggctcc
     4441 ctccgataga acaaatccct tacgatcagc actgaatggc ctcgaagcca gtgcgggatt
     4501 atcatatgcc cgatctttag gattaacttt tatggtggca ttcatattgg caaaaccgtg
     4561 aatcagctcc ggcagaatgg gcgtatcggc gccgccggcc agaacaaaat cgcaatcgcc
     4621 gtcccggatc attctcgccc cgatgccgat ggcatggttg ccggaggcgc aggcgccttg
     4681 cggcgaaaaa atcggtccgg taaatttcaa cagcatgcct gccttcccgg agggaagatt
     4741 cgcgcacaaa ttgggaagca gatacgggct gacgcgcagt ggtccgcggt gatccagatc
     4801 atgcacggca atgcgaaaag catccgaccc gttgagcgcc gaaccgatca gacaggccat
     4861 ccgcggagca atctgtgcgt cgacaatgat accggcattt tccagcgcct ccttgcaaac
     4921 ggccatcgtc aggatcacaa acgccgcgtt ccagttataa acttctttgg cgtcggtaaa
     4981 atcaagttcc atcggatacc agtccgggat ttctccgacc acgtcgcacg cggattccac
     5041 tttacagcgg gtaaccttgc ggaaaccggc ctcgcctttc accgccctct tccacgtttt
     5101 ttcaaacgtg ctgcccagcg gtgtggctgc accatacccg acaacaaata ctcttctgtt
     5161 tttgggcgct ctcataattt gcctttttta tgtcatttcg ggtgtcttga acaatctttt
     5221 ctttaaaatt gccatatcga ccaaggattg tctcgccatg acaatcatca ttctgttctg
     5281 tgcaagatga tgcaggaatt gcatccgccg aagccgaacg catttttcaa gacaaattcc
     5341 tgtttaaggt tcctcgcacc ttccgcgacg cagtctattg caatgtcctg atcaggacgg
     5401 taattgatcg tcggcaaaac aatctctttt ttcatgcctt ccatcgcgag gatggcttca
     5461 atggcgctgg aggcgcccat cgcatggccc atttgagatt tattcgccga taccggcggc
     5521 acatggccgc cgaaaatatt tttcaaagca tcggcctcca ctttatcgcc gatctttgtt
     5581 gacgtcgcat gcgcattaac cgcatcgatg tcctccggct ttaatccggc attggctatt
     5641 gtttcctcca tgcacttctg cacggttgcc aaattcgggg acacaaagtg actagcgtcc
     5701 gatgtcatgg cccatccggc gatttccatg ccggatggga gcccgtggac ttttacaaat
     5761 tcgcccgttg ccagaataat gcaaccggct ccttcggaaa cgacaaaacc gcgccggttt
     5821 gcagaaaacg gtctgctggt tttttccggc ggttcttcag gctgtccctc tttgggccgg
     5881 tatgcgccgt tcatcgtggc aaagcccgca acaatcggct ccaccagcgg aaaatccacc
     5941 gcaccggcaa tcaccacatc cgccatattt cgctgcaaca gcatctcgcc aatgatcatc
     6001 gatgtaatgc cggtagcaca ggccgtgatg gttgaacaaa tcggtcccgt tgcacccgtg
     6061 agaatggaga ccttgccgcc gaccatattg atgcaggaat tgggattggt aaacggatga
     6121 ggcagcttgc cgtccttcac catcaggcga tcggcctgca aaacggcatc cagccccccg
     6181 accgccgagc tgaatgtgat ggcgacccgg ggcgcgatgt cgcgcgtaat ttcaatcccg
     6241 ccgttctgca aagcgcgatg cacaaccagc agcgcgtatt tgaaaatggg tgacgtccag
     6301 tgcgccattt ctctgggctg caaaaacgga aacggacccg gatcgatttc ctccacctca
     6361 ccggccacgc gcaccggaaa attttcgttg agcgggaatc tggtcaaccg gccgatgccg
     6421 ctttgtccgg caaccgcttg ccgccattgt gcatccagct gcgcccccag tgacgatacg
     6481 gcatcataac ccacgatgac ggtttttttc aaccccatgc tccgacctga aaaataaatc
     6541 gatatcccct gaaaataaaa gggggatttt gatagaggga cctatatcaa aattttcggg
     6601 ggattaccac ggaataaagt gatagagctt ggcgaacatc tcgtaaactt cgatccagtt
     6661 ttgcagatct ttggttgatt tcagatgttc acgcttgttg accatgaccc agctcatatg
     6721 cgccgcgccg tctttgcagg tggagcaatc gcgtgtcgcg gacgtacagc agcgatgaac
     6781 ggttttcaga tcggatgccc agcgggcaaa atgaatcagc cggcggggac ggtttgctct
     6841 gttatccagg gcttcggtaa cggacggaca ttcactccag ccgaaatgtc gtcccatcat
     6901 tttcccggtg gtgatgattt catgatagta tttgcaggaa atgaccgtct gcgaataggt
     6961 gtccagcatc tcatccattt cgagccgaag atccttgagc tcgccttcct gccaggaaaa
     7021 accatcgaca ccttcgtcat tggataaaag ctgcaagtgg accttcaatc cgacatcggc
     7081 aatgcgtttg atgaccggtt caattttgcc gacctgccgc ggcgtaatgg tataaagata
     7141 ataggtatag gcatcccctt cgtaattttt actggagacg gcaaacgtgt cccgtccgcg
     7201 cagaattttt tcatcttcgg cgctgcccca aagcgagatg cccaccatca tatcggggaa
     7261 tctgtcacgc ggaactttaa tcaacccgtt ggtggcacaa tacgtaggca gccgtttata
     7321 aaacgcctcg acgcggtcca gacaaagcgt cggttcaccg ccgattagaa ttgccaggtt
     7381 cacgcccctt tctttttcgc gatcgataaa tgcttcccat tttccgatat ccatttcttc
     7441 gaccgccgcc tgatgctcgc ccgaagaaaa gaaaaaacaa cccttgcagc gnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttcgaccg ccgcctgatg ctcgcccgaa
     7621 gaaaagaaaa aacaaccctt gcagcgcaaa ttgcaccggt tggtcacgtc ataaatggaa
     7681 ctgcggatat tcagattgga aatccgtctg tatcgattat gccattcttt atccagcagg
     7741 gaactgactg ttttcatctt tactatccta aatcatgcca ttgcgacttt acataccatc
     7801 cggatgtcat ttcgaccgaa ggctatcgtc cggcgataat ggagagaaat ctttttcatt
     7861 aaatgatcgt tttaagattc ctcattcgcc gatgcggatt tgcaatgaca cctttgtttc
     7921 tatgaaaccg ttgcacgcgg cggttctatg atttcattac agagattctc acccttctca
     7981 tagttcatca cccagaccgc cagatacgta tcgacataat ccagccagga tttgaatgtg
     8041 gccagattcg tcacgtggcg gtagagtcgc gccgtcacca ctgcggagcc cgcagcgtaa
     8101 tggcggcagt ccgcgcaatc cgtatccggt acgcagcagg ctaccgaccg gtcccagttc
     8161 aggtccgttc tgtactgcct gaaagatctg cccagcccga tatcctgaga cggattctga
     8221 cggggatagg agcagctgta aagatcgtgc aggccttttc gatgcgtatg cgctatggca
     8281 ttgtaaacgg aaaaaagaac attgcgcgga tattgattta aaagttcaat catggtctgt
     8341 ctcgtttgct caagcgatgc atcatcatgc cggagatttc cggcatatcc gaccggcgca
     8401 gaaaacacat taaacgttaa tttgcagtca tgggcggcca gctcttccgt tacttcgtta
     8461 acctcatcaa tattattcct cgtaaatgta tagacaaaaa ccgcgcgcgg atcgttttcg
     8521 taattttcaa tctgtttttt cagaaggttt ttggcctttc tgattttcag gctggtttca
     8581 tcactgcccc agacggagat gtgcagttta taaccgacgg acggtggaat ctttctgaag
     8641 ccattggtgg caatgctgcc caacggcatt tcctgataac agacctccag caattccgga
     8701 acaagcgaag gttccgcgcc cgccagaacg acgtaggtta taccgcgtgc tttttccgct
     8761 ctcatcaatg cgcgccaggc ctccacatcg ccgacctctt tggcaaattg cttttcgcct
     8821 tcataataat agcagccgtc acagcggata ttacagcggt tcgtcatatc gtaggtcgat
     8881 tcgcgcagaa aaaaatactt tctcaccttc tcccagcgcg ctttgacttc gggacgggct
     8941 agaagctcgg aaaatttcgg tttgacggac tccgtattta ccttgttgtt tacctgattg
     9001 gccatctcgc attctgtcaa ttaatgtctg accggaagcc tttctttaat atattctgaa
     9061 atcagacgaa tggttttcaa ctggggataa tcatcctcat ctatggtaat tctgtattca
     9121 tcctgaagaa ccagtgcgat cgtggccagg tccatgctgt caatacccag ttcatccacc
     9181 agatccatat ccggatcaaa ggtatccgcg gtcgcttctt ccagcttcaa ctcatcaata
     9241 atgatttcgg ctacacgccg tataatgcct ttaacatctt gacttgcagg catcacttcc
     9301 ctccttacca tgcatattta tatttatttt tatcacatca tttttttggc agcaatcatt
     9361 ccgctgctga ccattgtatc tctgcaaaaa attttaaaat gaaacaagcg ttctccatcc
     9421 gcctctgccg cacggatatt caacgataac tgatcacccg gccttaccgg ttgcataaac
     9481 cggattctct tcaaagtatg caattcaagc cgctcgcctt tcttttgcgc ttcccggtga
     9541 atggcctgct ttaccgcata aaccagagca atgcccggaa gaatcggctc acccggaaaa
     9601 tgacctgaaa accaggggga gtctgccggg acctcggcta aggcctcgat atcattagaa
     9661 tcagcttctc ttgtttcttt taacaaattc cattgatttt tcaatcaata tacctcattg
     9721 gcaaatgccc ggcatacgtt cggggccaaa aagtataact gcatatcatt gtttgctttt
     9781 tcaatcaaaa attttttaca agatcaacaa ttaattatag atgacgcgat cttacatcat
     9841 tatggatacg tgcctgccga ttaaatcggc gaggggaacg acgtttaacc cctgtttttt
     9901 gagcccccgc aggatctttt cgatttcgat caataaaaca gcctggtttc ccccgccgcg
     9961 cggcaacgca tcgtgcagca ataaaatgtc gtctgctttg acgctgtgta atatccggga
    10021 acttaaattt ttaatgcgac ggttgccgaa atcaccgcct cggcaactga atgtcacgca
    10081 aaccattccc agtttatcca gaacggacgg cagtttagga ttgatgattc ccaccggcgg
    10141 ccggaaaatc aacgtattga caccccggtc tttcaggatg tcctgagctt tggatacttc
    10201 gttataaaga taacgataac tgcccagcat caaaaacgga ttgtgatgga atgaatgatt
    10261 gccgacggtg tgcccgcgtc ggatgatttc cctgacgatg tccggatgct ttaaagcatt
    10321 gacgccggaa acaaaaaaag tcgctttgac cgaatagcga tccagcagat ccagaatccg
    10381 tcttgtcgtc ggttcggccg gtccgtcgtc gaacgtcaga gcaacgacat tcctgccggt
    10441 tctcccccgg ctgatgacag gcaggtagaa actgctttgc gggaaaaacg aagcggcaac
    10501 gcacagaaca atataaaaaa gagcgacggc tgccgcggcc agtggatgaa agaaaaaaat
    10561 cacagccgcg gcaagcaaaa gaatgactcc ggtcatttca gcagggtttg cagatgacaa
    10621 tttctttttc atggataacc actacacggg tatggtgcat tccgtgaatg cgccgttttg
    10681 aactgtccgg ggaacagtcc ctgcaattct gatatttcag cctctcatat ttccggatgg
    10741 atcctcaagg gcacttcgtc cgcctctgcg ggcatgacaa aaaaacagcc ttgagttacg
    10801 gtccggatgt tttttaaaaa tttacgaagt tatattttta ctggatcaga tactcccata
    10861 acggtccggt gaatccgcgt ctgtaaagtt ttggcaggat gcgtgtggcc ttggcttcac
    10921 cggcgccgat cagccagttc aaccggccgt tggcatgatt ttccaccgtc ttcataatct
    10981 cttcaacacc gattccctga gaacggtaaa acacgggcgg cagaatattt tcccccgctt
    11041 tgatctggcc ttcgcgcacg gcaacatcat aaagcgccgt gtgcgggtag atgcgaatgc
    11101 cgcagaagaa aaagaagacc gctctttcga gcttatcgat tcccatcaga gttgcctgca
    11161 gcgtttcttt gttctcgccg ggaccgccca gcagaaaata atgcgccaca tataatccgg
    11221 cgtccagcgc ttcgcggtgt gccgtgaaaa cattttccga ggtgaagggc ttatgcagat
    11281 tctccaacac cggatcggat aaagattccg tgccaaattc cacgtgggtc aaaccggcat
    11341 ccgccagacg tttgaagtaa tcggcgggag gaaccgtgga ggcaaaaaaa cctccccagg
    11401 ggatagacac gcccgccttc ataaaagcct tcgccacctg accgctgtgt tcatagctgg
    11461 cattaaaggc ggaatcggta ataaaaatat atttggcgcc ggcatcctgt aatgcgcggg
    11521 cgctgcgggc gatctcctcc ggatcacagc accgcatcct gccaccctcg atatggggat
    11581 aggtgcagta actgcaacgg aagggacagc cccgttttgt ctggatattg agcattcccc
    11641 cgtacgcaag ataaaagcgc gtgtgcgttt cttcaggatc gaaacgtcgg ttgagtcttc
    11701 cgccaaaagg ttcgtagacg atatccgtca ttgctctggt caggacaccg ggaattctgt
    11761 tcacatcctc atgtttttcc cacgcgtcca gaagcagtgc aagtctttcg ccttcaccca
    11821 cgattccgta atcggcatcg agcacctgca tgaactcgcg gggaaaaatc gtgaaaccgc
    11881 tgccgcccag aatgatttta gccctggaat tttttctgat ctggccgact aaggtctcgt
    11941 attcgggaag aaagctgcgg cagttgatcg catcggtatt gtcgatgttt ctgatggaga
    12001 ttcccacgac atcgggagaa aattctctga tcattgtttc cagtgtttcc ggatgggaaa
    12061 aaacgttcag atcgacaatt tttgtccggt agcgggaatc caggcaaccg gccacatagt
    12121 ccagccccag cggatacacc ggatacgggt ttttcatagt attggcggaa atcaacagca
    12181 cgttcatcat aggaataaaa cacgcaaagg cggttgtttt gcgcaaaagg acattaaacc
    12241 gtttttttct gatgattgat gatatgatcg gccagcgcgc tgacggaagc gaaaaccttt
    12301 acgcccagtt tcttgctgtc gattttgacg ccataatcct tttcgatcat catgaccagt
    12361 tccagcacat cgatggaatc gattcccaga tcaccgccga ccagccgttc atcgtcctgg
    12421 atgtcatcgg ggccgacatc aatcaacccc agtgtctcga taattttgat tttcagttct
    12481 cttatcattt ctgcggcgtt actcattcgg ttctcctgtc ttgtcgatat ttttattcgg
    12541 cttggaggcg attttttttg cctcatctcc gagaattttc tccaacacca ccctcaccac
    12601 atattttccg agcgttttcg catattcggg accggtaaga tacatcccca tcatcatgat
    12661 caaccacgaa aaaccgtaag ccagaggaat gcctataaca ccaaccatcg gttttcgcaa
    12721 cctggcagcc ataaccccca ccatgaaaaa cgacggaagg acaacaatgt aacttaaaac
    12781 aatcagaacg attccgatgg tcacgcgcag cgtgtgtttc tctttcagaa gactcaaatc
    12841 cgcgcattcg ttaatggcgt tccggcagaa ttcttttcgg gcaaaataga ccgccgtttt
    12901 tttaacgata ttcaaaatat gtccttaaga aacccatgat caatatgata acgatatttt
    12961 ttcagcagaa ccggaagaac agccttgatg atcagacgga ttacctttcc ataatcccgc
    13021 cgattgaata tttcgaacgg agcggataat ctttgtaggc gatatcctta aaaacgccta
    13081 cgcgagcctg tccaaccgtg taaattaatt cattatccac ataaaggtgc ccgtcgccaa
    13141 tgacgatact tgcaccggat tccttcaaca tcgaataacg acggacatca acttcaaagc
    13201 gaacgcattt attaaaagga cgaatctggc cgttgaaagc aacatcgccg caacccagcg
    13261 ccctacccgt ccccaatgaa cctctccaca cacagaaaaa accaagcaat tgccataagc
    13321 cgtccactcc gagacatccg ggttgcactg gatcaccttt aaaatgacac tgaaaatacc
    13381 aggcatccgg tctgatatcc tgttccgcaa tgattttacc tttcttgccg tcgctttcaa
    13441 ttaataatat gcgatcaacc ataagaaaag gcggcgctgg aaatttcgcg tcaaaatgtt
    13501 ctggaggatc atcaaccagt ttaccgtaag caaaagcgag aatctcttca aaattaaagt
    13561 ggtctcgttt taaaaattcc tgatatttca taatggtttc ccgccttgct gaaaggtcaa
    13621 caacaa
//
LOCUS       DAOSME010000060        13306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_5414_length_13306_cov_19.140669, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000060 DAOSME010000000
VERSION     DAOSME010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13306
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5414_length_13306_cov_19.140669"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLG98_08800"
     CDS             <1..917
                     /locus_tag="PLG98_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33396.1"
                     /translation="LDNDQFIDTVYLGDLGGNMWRFRLCAKDANCSECGRTDYTSSPC
                     TSCTTSDWTGSLLFTSTNAERGANMATPSNAHKQIFTMAQATYDDNHNLWIFFGTGEN
                     NDPTYKPTEANPDTSETKNRLYAVKEDPNFTATYSSSQLENITSSRYTDSSEKHGWYI
                     NLSTNPLTRSDNTVISNPIGEKMISDPAIFGGMVYYATYVPDQEEDSACGRSGDAFLY
                     KLNYKTGAGGDANPNDPSDIGTRTSYMGHGIGSSILVSYAPEYSDADIYATASGGAGT
                     GALTQTMGKAPKASSMTNVLYWRDRRLQ"
     gene            1225..2889
                     /gene="argS"
                     /locus_tag="PLG98_08805"
     CDS             1225..2889
                     /gene="argS"
                     /locus_tag="PLG98_08805"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOE33397.1"
                     /translation="MKNKIINIMENALNRARQKGELPDVHLPYPEVEPPANREHGDYA
                     ANAAMILASQMKQNPRKIAQTILENIVDDEQIIDKAQIAGPGFINFFLKENVWHRAMK
                     VIEEQNDRYGRLDSGQGKNVQVEFVSANPTGPLHIGHARGAVVGDVIASLLHAAGYGV
                     SREYYINDAGNQMNNLGRSVLLRYREICGYPIEFPENCYQGDYIKEIARDILRQEGEK
                     YLKSEESVAIPYFTAVAGKTILDEIKVDLKDFGVVFDEYFSEKELYKDDGVNQLLNDL
                     RRKGFIYSEGETLWFKSTAFGDEKDRVVIRQNGDPTYFAADIAYHHHKYSRGFDMLID
                     VWGADHHGYMPRLWAGIQALGYDKPSLKIILVQLVNLLRAGEPVAMSTRSGEFVTLRE
                     VLDEVGRDAARYNFLMRRSDSHLDFDLELAKKQSSENPVYYVQYAHARICSIIRTAKE
                     RDIALPVYSDIESAVLKEADEMILIKMLVRYPELISGAANALEPHRITFYLNELAGVF
                     HSYYNKYKVVSDDATLTAARLFLVKMVKIVLQNALNILGVNAPEEM"
     gene            2984..3757
                     /locus_tag="PLG98_08810"
     CDS             2984..3757
                     /locus_tag="PLG98_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOE33398.1"
                     /translation="MATQNTKKFELKIGKTGVLIILGGMTGLLCAAFLLGVDIGKNIE
                     TYPGEIASLPQKALAMVWRPAKIKMAEQNVAQTGAPGPLPAEDNIDLTYHDTLMKKKG
                     LDETETFPEKTTPEAKAINEEELLKGKFHIETAVPIKPEAITPSKEEPAKKEMAALKE
                     SSPSPAGVTESRPDQAKFIVQVASLKDKSTAYRITGKIAALGFQTKIVKIDVKGQGSM
                     FRVFATGFKEKSQAQQAVKKINARTGMHCIIKKVDVRTN"
     gene            3787..5118
                     /gene="ffh"
                     /locus_tag="PLG98_08815"
     CDS             3787..5118
                     /gene="ffh"
                     /locus_tag="PLG98_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOE33399.1"
                     /translation="MFESLSEKLDNIFKKLKGRGVLNEENINASLKEIRMALLEADVN
                     FKVAKDFIEDVRVKAVGREVLESITPGQQIVKIVHDRLVELMGGSSATIKFGARIPSV
                     IMLVGLQGCGKTTTAAKLALLLSQEKKKICLVSADVYRPAAIEQLALLGKQIHTGVFE
                     DKNLKDPVEICAQAVEFAKKNGYEVLLIDTAGRLHIDNLLMEELQKIKSRVNPAEIIY
                     VADAMTGQDAVNVGVKFNELIGIDGVIMTKMDGDARGGSALSLKSVIGKPIKFVGVGE
                     KIDNLEVFHPERMASRILGMGDILSLVEKAQANIDKKTALELEKSIRKNSFSLEDFRN
                     QLVQIKKMGSIKDIMGMIPGLGKMKALKDMEPDDKQLVKITAIIDSMTRKERHNYLII
                     DGRRRKRIAMGSGTTVQDVNLLLKNYMEIRKMMKKFNKKDGIKSLMRNFPF"
     gene            5184..5438
                     /gene="rpsP"
                     /locus_tag="PLG98_08820"
     CDS             5184..5438
                     /gene="rpsP"
                     /locus_tag="PLG98_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOE33400.1"
                     /translation="MAVKIRLARMGAKGKPFYRIVAADSEFPRDGRFLEILGNYDPKK
                     NPAEVLVKEERIRKWLQKGAKATLTVANLLKAKGIEAKIK"
     gene            5524..5754
                     /locus_tag="PLG98_08825"
     CDS             5524..5754
                     /locus_tag="PLG98_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOE33401.1"
                     /translation="MKELIKYISQALVDYPDKVEVTEIIGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAIRMILSAASAKAHKRAVLEIIE"
     gene            5762..6262
                     /gene="rimM"
                     /locus_tag="PLG98_08830"
     CDS             5762..6262
                     /gene="rimM"
                     /locus_tag="PLG98_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOE33402.1"
                     /translation="MDLFEVGEIVKTRGLCGCLKILSYLKSGNKISKPDVVYIEKTPG
                     QKNRFCLNKMEQSRNALFIELEGINDVNSAGHLIGGRVYFSKDVLKKLPAGEYYVHEI
                     IGLDVYSDAGDYLGKIESVFPTGSNDVYVCRKAKEEILLPAISDVIKRIDLDQRIMTV
                     KIPDGL"
     gene            6265..7005
                     /gene="trmD"
                     /locus_tag="PLG98_08835"
     CDS             6265..7005
                     /gene="trmD"
                     /locus_tag="PLG98_08835"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOE33403.1"
                     /translation="MIHFDILSIFPDMFTSPLGCSLLKKAQEKGIIHVECHDIRKWTS
                     GRHNMTDDAPYGGGCGMVMKPEPVEKALSAIRKKHQEEPTVILMTPQGEVLNQQIATE
                     LSEKKRIIIICGRYEGVDERIRQHLVDREISIGDYILTGGELPAMVLIDAVSRLLPEF
                     LGNPESTFTESFSQNLLEYPQYTRPAVYKGWKVPEVLLSGNHSEIEMWRKTESLRKTR
                     RKRPDLLKKMKLSDQDRKLLEKIKMEDI"
     gene            7085..7639
                     /locus_tag="PLG98_08840"
     CDS             7085..7639
                     /locus_tag="PLG98_08840"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE33404.1"
                     /translation="MLYIALLHYPVYDKNGRIVTTAIANMDIHDIARVAKTYGAAGFY
                     IVNPAARQRRLAQDIVNHWQEGFGAQYNPHRQNAFSLIHIRESLQNVVDDIVRRTGRK
                     PKSVATGAGSAQGDVLTFAELKDCLKKENDVYMLLFGTGSGISEKLIHESDFRLEPIK
                     GMAGYNHLSVRSAVAVIMDRIMRD"
     gene            7670..8017
                     /gene="rplS"
                     /locus_tag="PLG98_08845"
     CDS             7670..8017
                     /gene="rplS"
                     /locus_tag="PLG98_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOE33405.1"
                     /translation="MNILELTEKEQTRGDIPAFKTGDTVKVYVRIIEGQKQRIQAFEG
                     VVIRKRHGNSRANFTVRKISYGVGVERTFPLHSPIIDQIDVVSRGKVRRSRLYYLREL
                     RGKKARIKDAAKS"
     gene            8090..8707
                     /locus_tag="PLG98_08850"
     CDS             8090..8707
                     /locus_tag="PLG98_08850"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOE33406.1"
                     /translation="MDTFEKIARKEGYHFIAGVDEVGRGPLAGPVVAAAVVFPPDYIH
                     SEIKDSKTLSSPKREKLYNVIENDALAIGMKVVDVETIDQVNILQASLQAMRDSVLEL
                     DVSPDYLLVDGLYPVPIITPQKPLVRGDSLSVSIAAASIIAKVTRDRIMEMYHRQFPQ
                     YNFRKNKGYGTREHLNAIRTYGICKIHRKSFHVKNLHQASFEFNG"
     gene            8717..9088
                     /locus_tag="PLG98_08855"
     CDS             8717..9088
                     /locus_tag="PLG98_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOE33407.1"
                     /translation="MTKIITGKKGERLAADFLTGNGYQILETNFRCPLGEIDIIARDH
                     QEIVFVEVKTRKSHALGYPEQAVGIQKQKKLSQLALWYLQAKKMSDKKARFDVVAVTL
                     AVQANEINLIKNAFDFVDACP"
     gene            complement(9095..10471)
                     /locus_tag="PLG98_08860"
     CDS             complement(9095..10471)
                     /locus_tag="PLG98_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOE33408.1"
                     /translation="MAIRKKILCIGAGYVGGPSMAMIAHKCPDYRVTVVDINPERIDA
                     WNSEQLPVFEPGLDTIVKKTRGKNLFFRKDIENQIRDNDIIFVSVNTPTKSYGAGAHM
                     AADLQYVEKTARQILKNSQSSKIVIEKSTLPVKTALAMEKILSSRKGKIRFDVLSNPE
                     FMAEGTAVKDLENPDRVLIGSRETPGGLKARDHLIKIYTRWVPLNRIITSSIWSSELS
                     KLVANAFLAQRVSSINSISALCEKTDADIDEVARAVGMDKRIGDRFLKAGIGFGGSCF
                     KKDILNLVYLCNHYGLRDVAAYWENVLRINEYQRERFVNHILHSMFGTLSEKKICILG
                     FAFKADTGDTRESPAFYITQKLVAEQADVVISDPRALKNAAADLSGIKGNVKFVDDPY
                     RAAADCHAVLILTDWDLYRRLDYRKIYRSMIKPAFLFDGRNILDHQQCFSIGFNVYPI
                     GKRALTHF"
     gene            complement(10493..11437)
                     /locus_tag="PLG98_08865"
     CDS             complement(10493..11437)
                     /locus_tag="PLG98_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE33409.1"
                     /translation="MTKRILITGGAGFIGSHLCDKLLAQGHDVICLDNFFTGDKNNIA
                     HLLGHKRFELIRHDIINPVYLEVEEIYNLACPASPVHYQYNPIKTIKTNVMGVINVLG
                     IAKRTKAKILQASTSEVYGDPDVHPQNESYWGKVNPVGIRSCYDEGKRAAECLMMDYR
                     RQNGVNTKIVRIFNTYGPRMALHDGRVVSNFVIQALQNKVITVYGDGSHTRSFCFVDD
                     MITGLIRMMNAPDDFYGPVNLGNPEEFTILELAQTVIRLTNSRSRIEFLPLPQDDPVQ
                     RRPDISLAKAGIGWWPQISLEEGLLKTIDYFRQVLKNT"
     gene            11638..11949
                     /locus_tag="PLG98_08870"
     CDS             11638..11949
                     /locus_tag="PLG98_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33410.1"
                     /translation="MDANSITDDDKIIHDLKDELPDNAENNQEVIVVDGRSYENGSIA
                     KDVYTLVDVIEDHQIGDKIYEDILKRADEIVERIVRRIVPEIAERVIKEEIEKIKNEP
                     S"
     gene            11971..12564
                     /locus_tag="PLG98_08875"
     CDS             11971..12564
                     /locus_tag="PLG98_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33411.1"
                     /translation="MTYFKNIVFLVLGMLILSGCFGNTQKSIKDETAKLSIKSIAILP
                     IETATDADVKDAQFLRTRIFEEIFFKGYPKISPEDVDQRLGQAPGNADDKTSSMPPQA
                     LTDKIDAETVMSCSLKKNTQYKLFYASTTADVSCVLRRTETDDVVWKTQTHAAETSFA
                     LGQKRLEIKAHEAVEPLINKILKKIMETLPDGPNFIH"
     gene            12622..13170
                     /locus_tag="PLG98_08880"
     CDS             12622..13170
                     /locus_tag="PLG98_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOE33412.1"
                     /translation="MNIPNLISLLRIILVPVIVIFLIQEEYANALIAFSIAGITDALD
                     GALARLLKKQTELGAFLDPLADKILLSTSFIALSIFGLIPGWLTVIVISRDLIIVIGI
                     ITLTMMSVTYEIKPVFISKITTALQIGTVFIALFVKIFPFELMRIDFINVLSWGTAFF
                     TVVSGLVYIFKGIKFLNHSSEK"
BASE COUNT         3799 a         2923 c         3269 g         3315 t
ORIGIN      
        1 atctggataa tgaccagttt atcgataccg tatatctcgg agatctgggc ggaaacatgt
       61 ggcggttccg gctctgtgcc aaagatgcaa attgttccga atgcggaagg acagattaca
      121 cctcttcgcc ctgtacctcc tgcacaacct ccgactggac gggaagcctt ctgtttacgt
      181 cgaccaatgc cgaaagaggg gcaaacatgg caacgccaag caatgcccat aaacagatct
      241 ttacgatggc gcaggcaacc tacgacgata accacaatct gtggatattc ttcggaaccg
      301 gcgaaaacaa tgatccgact tataaaccga cggaagccaa tccggatacc agcgaaacaa
      361 aaaacagact ctacgctgtt aaggaggacc ccaattttac cgctacgtat tcatcctcac
      421 agctggaaaa catcacgtct tcccgttata ccgattccag cgaaaagcac ggctggtaca
      481 ttaatctttc aaccaatcct ctgacgcgtt cggataacac ggtcatttca aatcccatcg
      541 gagaaaagat gatctcggat ccggcaattt tcggcggaat ggtgtattac gccacctatg
      601 tgcccgatca ggaggaagac agcgcctgcg gtagatccgg cgatgcgttt ctctataagc
      661 tcaattacaa aaccggtgcg ggaggagacg cgaatcccaa cgacccaagt gatatcggta
      721 ccagaaccag ttatatgggg catggcatcg gctcctccat tcttgtttct tatgctccgg
      781 aatactcgga tgcggatatc tatgccacgg ccagcggcgg tgccggcaca ggagcgctga
      841 cccagacaat ggggaaagcg ccaaaggctt ccagtatgac caatgtcctt tattggcggg
      901 accggcgttt gcagtaaaga tccatatgac gtaacacagg acagagatat cataaaaaaa
      961 taactgacat ggcggcaaat tcggaatcat taccggattt gccgccatca cgttacagat
     1021 ttttttaaaa tgaataaatg ccgacggatg agacgctaaa aggccggcaa aaccgattac
     1081 aacacactca tccccgatga taagcatgtt gacgacattt taattattta ttaactcatt
     1141 aagaaaaata tggttgagct tgcgatgaca ggcggcacgt gataatgaaa gctgcttgtc
     1201 atttttttac aggttgcaaa aattatgaaa aataaaatca tcaacattat ggaaaacgct
     1261 ctgaatcgtg ctcggcagaa aggagagctt cccgatgtcc atctgcctta tccggaagtc
     1321 gaaccgcccg ccaaccgcga gcacggcgat tatgccgcca atgccgccat gattctggcg
     1381 tcgcagatga agcagaatcc gcggaaaatc gcgcagacga ttctggaaaa catcgtcgat
     1441 gatgaacaga tcatcgataa ggcccagatt gccggccccg gttttattaa tttctttctt
     1501 aaggaaaacg tttggcaccg ggccatgaag gtcattgaag aacaaaacga tcgctacgga
     1561 cgcctggatt cgggccaggg gaaaaacgtt caggtggaat ttgttagtgc aaatcccacg
     1621 ggaccgctgc atatcgggca tgctcgcggc gcggtggtcg gcgatgtcat cgccagtttg
     1681 ttacatgcag ccggttatgg cgtttcccgc gaatattaca ttaacgatgc cgggaatcag
     1741 atgaataatc tgggcagatc ggtgctgctg cgctaccggg agatttgcgg ataccccatt
     1801 gaatttcctg aaaactgtta tcagggcgac tacatcaaag agattgcccg ggatattctt
     1861 cgtcaggaag gggaaaaata tcttaagtcc gaagagtcgg tcgccattcc ctattttacc
     1921 gccgtggcag gaaaaaccat tcttgacgaa atcaaggtcg atttaaagga tttcggcgtt
     1981 gtttttgatg aatatttcag tgaaaaggaa ctgtataaag acgacggcgt aaatcaattg
     2041 ctcaatgatt tgcgccggaa aggctttatt tattccgaag gtgaaacgct ctggtttaaa
     2101 tcaacagcct tcggcgacga aaaggaccgg gtcgtgatcc gccaaaacgg agatccgacg
     2161 tatttcgccg cagatattgc ctatcatcat cacaaatatt cacgcggttt tgatatgctg
     2221 attgatgtct ggggcgccga tcaccacggc tacatgccga ggctctgggc gggcattcag
     2281 gccttgggat acgataagcc atccctgaaa atcattctgg tgcaactggt aaatcttttg
     2341 cgggccggag aaccggtggc catgtccacc cgctccggag aatttgtaac gttgcgcgaa
     2401 gtcctcgatg aagtcggaag agatgccgcc cgttataatt ttctaatgcg gcgttccgac
     2461 agccatctgg attttgatct ggaactggcc aaaaaacagt ccagcgaaaa tccggtttat
     2521 tatgtccagt atgcccatgc ccggatttgc agcattattc ggacggcaaa agaacgcgat
     2581 atcgctctgc ccgtttacag cgacatcgaa tccgccgtgc tcaaagaagc cgatgaaatg
     2641 attttgatca agatgcttgt ccgttatccg gaattgattt ccggagcggc aaacgctctg
     2701 gaaccgcacc gcattacatt ttacctcaac gaactggccg gtgtttttca cagttattat
     2761 aataaatata aagtcgtctc agacgatgcc acgctgacgg cggcaagact ttttctggtg
     2821 aagatggtta aaattgtttt acagaacgct ttgaatattc tgggggtaaa cgctccggaa
     2881 gaaatgtaac ttttttaaaa ttccgggaaa atcaaccggt tgtttgactg taataacacg
     2941 ctgaaatttt ggaaaatgtc atttttccgg tttgaaaaga ttcatggcta cgcaaaatac
     3001 gaaaaaattt gaattaaaaa tagggaagac gggcgtcttg attattcttg gagggatgac
     3061 cgggctttta tgcgcggcgt ttcttctggg cgttgatatc gggaaaaata tcgaaacgta
     3121 cccgggagaa atcgcgtctc tgccgcaaaa agcgctggcg atggtctggc gtccggccaa
     3181 gattaaaatg gccgagcaaa acgtcgcgca aaccggagcc cccggacctc ttcccgccga
     3241 agataacatc gacctgactt atcatgatac gctcatgaaa aagaaggggt tggacgaaac
     3301 ggaaacattt cccgaaaaaa cgacgccgga agccaaagcg atcaatgaag aagaactttt
     3361 gaagggcaaa tttcatattg aaacggcggt gccgataaaa cctgaagcaa tcacaccctc
     3421 taaagaagag cccgccaaaa aggaaatggc cgctttaaag gaatcctctc catcgccggc
     3481 aggcgtaacg gaatcccgtc ccgatcaggc caaatttatc gtgcaggtgg cttcgctgaa
     3541 ggacaaatca acggcgtatc ggataaccgg gaaaatcgcc gccctgggtt ttcaaaccaa
     3601 aattgtcaaa attgacgtga aagggcaggg aagcatgttc cgggtttttg caacgggatt
     3661 taaagaaaaa agtcaggcgc aacaggccgt caaaaaaatc aatgccagga cgggcatgca
     3721 ttgcataatc aaaaaagtag atgtcaggac aaactaaaat aataaggatg aaatggttga
     3781 agggttatgt tcgaaagtct ttctgaaaaa ctggataata tttttaaaaa acttaaaggg
     3841 cgcggcgttt taaacgagga aaatatcaat gcctcgctca aggaaatccg catggccctc
     3901 ctggaagcgg atgtcaattt taaagtggcc aaagatttca tcgaagatgt gcgtgtcaag
     3961 gccgtgggcc gggaagtcct cgaaagcatc acccccggcc agcagatcgt caagatcgtt
     4021 catgaccggc tggtggaact catgggcggc agcagcgcca caataaaatt cggagcgcgc
     4081 attccgtcgg tcattatgct ggtcggcctg cagggctgcg gtaaaacaac cactgccgcc
     4141 aagctggcct tgctgttatc ccaggaaaag aaaaaaatat gtctggtttc tgcggacgtt
     4201 tatcgtccgg cggcaatcga acagctggcg ctgcttggta aacagatcca caccggcgta
     4261 tttgaagata aaaacctgaa agaccccgtg gagatttgcg ctcaggccgt ggaattcgcg
     4321 aaaaaaaacg ggtatgaagt gctgctgatc gacacggcgg gccggctgca tatcgacaat
     4381 ctcctcatgg aagaactcca aaaaattaaa agcagggtca atcccgctga aattatttat
     4441 gtcgccgacg ctatgaccgg acaggatgcc gtcaatgtgg gagtcaaatt caacgaattg
     4501 atcggaatcg acggcgtgat tatgaccaaa atggacggcg atgcccgtgg cggcagcgct
     4561 ttgtccttga aatcggtgat cggaaagccg atcaaatttg tcggggtggg agaaaaaatt
     4621 gacaatctgg aagtttttca tcccgaaaga atggcctcgc gtattctggg aatgggcgac
     4681 attctttccc tggtggaaaa ggcgcaggcc aatatcgata aaaaaacggc tctggagctc
     4741 gaaaaaagca tccgcaagaa cagtttttcg ttggaggact ttcgcaacca gctggtgcag
     4801 attaaaaaaa tgggatccat caaggatatt atgggaatga ttcccggcct gggaaaaatg
     4861 aaagccctca aagatatgga gccggatgat aaacaactgg tcaaaattac cgcgatcatc
     4921 gattccatga cccgaaagga gaggcacaat tatctgatta tcgacggcag gcggcgcaaa
     4981 agaatcgcta tgggcagcgg aaccaccgtt caggatgtga atcttttact gaaaaattac
     5041 atggaaataa gaaaaatgat gaaaaaattt aataaaaaag atggaataaa atctctgatg
     5101 cggaattttc ccttttaata taaattaaag tatgataaaa aaatagaaaa attattattt
     5161 taaggaggta ataggacaaa tcaatggctg ttaaaattag actcgcccgg atgggtgcca
     5221 aaggcaaacc tttttatcga attgtggcag cggacagcga atttccccgc gatggaagat
     5281 ttctggaaat tttaggcaat tacgatccca agaaaaatcc tgcggaagtc cttgtcaagg
     5341 aagaacgcat tcgtaaatgg ttgcaaaagg gtgccaaggc aactctgaca gtcgctaact
     5401 tgctgaaggc aaaggggata gaagccaaga tcaaataatt ttttaatcac aatgtttggg
     5461 gtgtgttctg tatcggaaat tatttctaga aggacttaat taaactcagc ggaggttatg
     5521 acgatgaagg aattgatcaa atatatatct caggctttgg tagattatcc ggataaggtg
     5581 gaagttactg aaatcatcgg cgaacaaaca tctgttattg aactgcgcgt ggccaaagaa
     5641 gatctcggta aagtcatcgg caaacaggga aggaccgcca aggcgatccg gatgatttta
     5701 agcgcggctt ccgccaaggc gcataagcga gctgttttag aaataatcga ataggcagac
     5761 catggatctc tttgaagttg gagagattgt caaaacccgc ggtctttgcg ggtgtttgaa
     5821 aattttatct tatcttaaaa gcggaaataa aatatcaaaa cccgatgttg tgtatattga
     5881 aaaaactccg gggcaaaaaa atcgtttttg cctgaacaaa atggaacaat cccgcaacgc
     5941 gttatttata gaactcgagg ggattaacga cgtaaactcg gccgggcatc tgatcggcgg
     6001 ccgggtttat ttttctaaag atgttttaaa aaaacttccg gccggggagt attacgttca
     6061 cgaaatcatc ggccttgatg tttacagcga cgcgggagat tacctcggca aaatcgaatc
     6121 ggtttttccc accgggagca atgatgtgta tgtctgccgg aaggcaaaag aggagatcct
     6181 cctgccggca atttccgacg ttataaaacg gatagatctt gatcagcgga ttatgaccgt
     6241 caaaattccg gatggattgt aaacgtgata cattttgaca ttctttccat tttcccggac
     6301 atgtttacgt cgcccctcgg ctgcagtctt ctgaaaaaag cgcaggaaaa agggattatt
     6361 catgtagaat gtcatgacat acgcaaatgg accagcggca gacataacat gaccgatgac
     6421 gctccctacg gcggcgggtg cggtatggtg atgaagccgg aaccggtgga aaaggcgttg
     6481 tcggccatca gaaagaaaca ccaagaagaa ccgactgtta ttctgatgac gcctcagggg
     6541 gaggttctta atcagcagat cgcaacggaa ctttctgaaa aaaaaaggat catcatcatc
     6601 tgcgggcgct atgagggcgt tgatgaacgc atcagacagc acctggtgga ccgtgaaatt
     6661 tctatcggcg attatattct gaccggaggg gagttgccgg caatggtgct gattgatgcg
     6721 gtttcccgtc ttctgccgga attccttggc aatccggaat cgacattcac cgaatctttt
     6781 tcacagaatc ttctggagta cccccaatac acacggccgg ccgtctataa gggctggaag
     6841 gtgccggagg tattgctttc cggaaatcat tctgaaatcg agatgtggcg caaaacagaa
     6901 tcgttgagga aaacccggcg gaagcgaccc gatttgctca aaaaaatgaa gctctccgat
     6961 caggacagaa aactgcttga aaaaattaag atggaagata tataaaacct tgattttcag
     7021 aaatatttaa tgtatgggtt tccgcttgaa tgtgcgggat caatgatgtc acacggcaaa
     7081 ggaaatgctt tacatcgctc ttttacatta tccggtatac gataaaaacg gccggattgt
     7141 cacgacggcc attgccaata tggacattca cgacattgcc cgggttgcca aaacgtatgg
     7201 cgccgccggt ttttatatcg tcaacccggc agcaagacaa cgccgtctgg cgcaggacat
     7261 tgtcaatcac tggcaggaag gattcggtgc gcaatataat cctcatcgtc aaaacgcttt
     7321 cagcctgatc catatcagag agagtttgca gaacgttgtt gatgacatcg tccgtcggac
     7381 agggcgtaaa ccgaagagcg tggcgacggg tgccggttct gctcaaggcg atgttttaac
     7441 gtttgccgaa ttgaaagatt gcctgaaaaa ggaaaatgac gtttatatgc ttctttttgg
     7501 cacaggatcc ggaatctccg aaaaactgat tcacgagtcg gattttaggt tagagccgat
     7561 caaggggatg gccggttata atcatctgtc ggtacggtcc gccgtagccg ttattatgga
     7621 cagaatcatg agagattgat tttttaagca aaatttgcgg gaggaaaaca tgaatatttt
     7681 ggaattgact gagaaagaac aaaccagagg ggatatcccc gcttttaaaa caggggatac
     7741 cgtcaaagtt tatgtcagaa ttattgaagg ccagaaacag aggattcagg cgtttgaagg
     7801 tgtcgtcatc cgtaaaagac acggaaacag ccgggccaat tttacggtca gaaaaatctc
     7861 ctacggtgtc ggtgttgaaa gaacgtttcc cttgcattca ccgatcatcg atcaaattga
     7921 tgttgtcagc cggggtaagg tgcgccgctc gcgcctctat tatttaaggg aattacgggg
     7981 caaaaaagcc agaattaaag acgcggccaa atcataaaaa ttgttctgcc cgagagcggc
     8041 gtgccttcac aggtacgcaa gctcttggat cggggatgta tcttccgaaa tggatacttt
     8101 tgaaaaaatt gcccgcaaag aaggatatca ttttattgcc ggtgttgatg aggtgggcag
     8161 agggccgttg gccggtccgg tcgttgcggc tgcagtcgtg tttcctcccg attatatcca
     8221 ttctgagata aaagattcta aaacactttc ctctccaaaa agagaaaaat tatacaacgt
     8281 cattgaaaac gatgccctgg ccatcgggat gaaggtcgtt gacgttgaaa caatcgatca
     8341 agtgaatatt ctgcaggcgt ctttacaggc catgcgcgac tctgttctcg aactcgatgt
     8401 ttcgcccgat tatctgctcg tcgacggttt gtatcctgtg ccgatcatca cgccccagaa
     8461 gcctctggtt agaggagaca gcctcagcgt ctccattgct gctgcctcaa ttatcgccaa
     8521 agtgacacgg gatcggatta tggaaatgta tcatcgtcag tttccgcaat ataatttccg
     8581 gaaaaataaa ggctacggaa cccgggagca tctcaacgcc atcagaacat acggcatctg
     8641 taaaattcac cggaaatcat ttcatgttaa aaatcttcat caggcatcat ttgagtttaa
     8701 cggttaaatg caaaacatga caaaaatcat taccgggaaa aaaggggagc ggctggctgc
     8761 tgattttctg accggcaacg gttatcagat cctggaaacc aatttccgct gtcctctggg
     8821 ggagattgac attatcgccc gggatcatca ggagatcgtt tttgttgagg tcaaaacaag
     8881 aaaatcgcat gctctgggat atcccgaaca ggctgtcggc atccaaaagc aaaaaaaatt
     8941 gtctcaattg gccttgtggt atcttcaagc caaaaaaatg tccgacaaga aagcacgttt
     9001 tgatgtcgtg gcggtcaccc tggccgttca ggccaatgaa attaatctga taaaaaatgc
     9061 cttcgatttt gttgacgcct gcccgtaaac tattttaaaa atgcgtcagc gcccgttttc
     9121 cgatgggata aacattgaac ccgatggaga agcactgctg atgatccaga atgttgcggc
     9181 cgtcaaaaag aaaggccggc ttgatcatgg agcgatatat tttccggtaa tccagtctgc
     9241 ggtataaatc ccagtccgtt aaaatgagca ccgcgtgaca gtccgcggca gctctgtaag
     9301 gatcgtcgac aaattttaca tttcctttga ttcccgacag atctgcggcg gcatttttca
     9361 atgcccgggg atcggaaata acaacgtcgg cctgttccgc caccagtttt tgcgtaatat
     9421 aaaaagcggg gctttccctc gtatcgcctg tatcggcctt gaaagcaaaa cccaggatgc
     9481 agatcttttt ctccgacagc gttccgaaca tgctgtgaag aatatgattt acaaaacgct
     9541 cccgctgata ttcgttgatg cggaggacat tttcccagta ggcggcgacg tcacgcaatc
     9601 cgtaatgatt gcacaaatac accagattca gaatgtcttt tttaaaacag gatccgccga
     9661 aaccgatgcc ggccttgaga aatctatcgc cgattcgttt atccatgccg accgccctgg
     9721 cgacttcatc gatgtcggcg tctgtttttt cgcataacgc agatatggaa ttgatggagg
     9781 acacccgctg cgcgaggaag gcgtttgcga ccagtttgga caattcgctg ctccagatgc
     9841 tggaggtgat gatcctgttt aagggaaccc aacgcgtgta aattttgatg aggtgatctc
     9901 tcgcctttaa tccgccaggg gtctcgcgcg agccgatcaa aacgcggtcg ggattttcca
     9961 gatctttcac ggcggtgcct tcggccatga attcgggatt ggagagaaca tcaaaccgga
    10021 tcttcccttt tcgggaggat aaaatctttt ccatcgccag cgcggttttt accggcaagg
    10081 tgcttttttc gattacgatt tttgacgatt gcgagttctt taaaatctgg cgcgcagttt
    10141 tttccacata ttgcagatcg gccgccatgt gtgctccggc gccgtagctt ttcgtcgggg
    10201 tgttgacgct gacgaaaata atgtcattgt cccggatttg attttcaatg tcttttctga
    10261 aaaataaatt tttgccgcgg gtttttttta caatcgtatc gagaccgggt tcgaaaacgg
    10321 gcaactgctc cgaattccac gcatcaatcc gttcaggatt aatatccacg acggtcaccc
    10381 ggtaatcggg gcatttgtgg gcgatcatcg ccatggaagg gccgccgaca taaccggctc
    10441 cgatgcaaag gatttttttt ctgatagcca tttatttctt acctctgtgc gtttatgtat
    10501 tttttaatac ctgacgaaaa tagtcaatgg ttttgagcaa cccctcctcg agactgattt
    10561 gaggccacca gccgatgccg gcttttgcca gcgagatatc cggcctgcgt tgaacaggat
    10621 cgtcttgagg caggggcagg aattctattc tggagcggct gtttgtaagc cggataacgg
    10681 tttgcgcgag ttccagaatg gtaaattctt ccgggttgcc gagattgacc ggaccgtaaa
    10741 aatcgtcggg ggcattcatc atccggatga gcccggtaat catatcgtcg acaaaacaga
    10801 atgatcgcgt gtgagaaccg tcgccgtaga cagtgatgac tttgttctgc agggcctgaa
    10861 tgacaaaatt gcttaccacg cggccgtcat gcaacgccat ccgcggaccg taggtgttga
    10921 agatgcggac gattttcgtg ttgacgccgt tttgccgacg ataatccatc atcaggcatt
    10981 ctgccgctct tttgccttca tcatagcaac tgcggatgcc gaccggattc actttccccc
    11041 agtaggattc gttctgcgga tggacgtccg gatcgccgta cacctccgat gtggacgcct
    11101 gaagaatttt ggcttttgtc ctcttggcga tgccgagaac attaattact cccatgacgt
    11161 tggttttgat cgttttaatg ggattgtact ggtaatgaac cggagacgcg ggacaggcaa
    11221 gattgtatat ttcttccact tccagataaa caggattgat gatgtcatga cggatcagct
    11281 caaaacgttt atgtcccaga agatgagcaa tattattttt atcgcctgtg aaaaaattat
    11341 ccagacagat gacgtcatgc ccctgtgcca gaagtttgtc gcaaaggtga gaaccgataa
    11401 aacctgcacc acctgtaatc agtatccgtt tcgtcatttt ttcaattcac aatttatttg
    11461 gcgaatatta atatgaaata attcgggaat gtcaacaatt aaaatagcat attgatggta
    11521 ttttttcatg ccgtccgatt atgatatata tcttgaactg ttttatgctc tatgataaca
    11581 aaccaacacg ctgcaaattt accgtgtttg gttaagcgtc tggacaagga gatgacgatg
    11641 gatgcaaatt ccataaccga tgacgataaa atcatccatg atcttaagga tgagttgcct
    11701 gataatgctg aaaataatca ggaagtgatt gttgttgacg gacggagtta cgaaaacggc
    11761 tccatagcaa aggacgtgta tactctggtg gatgtcattg aagatcatca gatcggggat
    11821 aaaatctacg aagacattct caaaagagcc gacgagatcg tggaacgtat tgtccgcagg
    11881 atcgttccgg aaatagcgga aagagtgatc aaggaagaaa ttgaaaaaat caaaaatgaa
    11941 ccgtcatgac gaatagagtt taaagaggat atgacctatt tcaaaaatat cgtttttctt
    12001 gttctgggga tgctgattct gagcggatgc ttcggcaata cgcaaaaatc aatcaaggat
    12061 gagacggcaa aactcagcat aaaatccatt gccatccttc cgatagaaac cgccacggat
    12121 gcggacgtta aagacgctca gttcctccgt accaggattt tcgaagaaat tttttttaag
    12181 ggctacccca agatatcccc ggaagacgtg gatcaacgcc tgggccaggc gcccggcaat
    12241 gccgacgaca agacatcttc catgccgccg caggctctga cggataagat agacgcggaa
    12301 acggtcatgt cctgctcctt gaaaaaaaat acgcaatata aattgtttta tgcgtcgaca
    12361 accgctgacg tgagttgtgt gttgcgccgc acagaaacgg atgacgttgt ttggaaaacg
    12421 cagacacatg ccgcagaaac gagttttgcg ttgggccaaa agagactgga aatcaaggcg
    12481 catgaagcgg ttgaaccgct gatcaataaa attctgaaaa aaataatgga aacattgccc
    12541 gacgggccga actttattca ttaagcaaag caaaacagca ttacgaaaag accggcaaat
    12601 tatttttgaa atgactatgc gatgaatatt cccaatctga tatctttgct cagaattata
    12661 ctggtcccgg tgattgtcat attcctcatt caggaagaat atgcaaacgc gttgattgca
    12721 ttttccatcg ccggtatcac ggatgcactg gacggtgcgc tggccagact cttgaagaag
    12781 caaaccgaac tgggcgcttt tctggatccg cttgctgata aaattctttt atcgacgagt
    12841 tttattgcct tgtccatttt cggattgatt ccgggatggc tgactgtcat tgtcatcagc
    12901 agggatctga tcattgtcat cggtatcatc acattaacga tgatgtcggt aacgtatgaa
    12961 attaaaccgg tttttatcag caaaatcaca acagctttac aaatagggac cgttttcatt
    13021 gctctttttg ttaaaatatt tccatttgaa ttaatgcgca ttgatttcat taacgttctt
    13081 tcgtggggca ccgctttttt tacggttgtc tccggattgg tatatatatt caagggaata
    13141 aagtttttaa atcacagctc agaaaaatag gctaataaat aagacattta gcttgacacg
    13201 gcaatatttt gctgctatca acacgtcgtt aaaaaaatcc ggtttttacc gtaggcaagt
    13261 agctcagggg gagagcgcca tcctgacgcg gtggatgtcc agggtt
//
LOCUS       DAOSME010000061        11768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6293_length_11768_cov_23.233672, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000061 DAOSME010000000
VERSION     DAOSME010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11768
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_11768_cov_23.233672"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PLG98_08885"
     CDS             <1..1196
                     /locus_tag="PLG98_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOE33413.1"
                     /translation="AETKTVPKRSSTAKWRPSRDVMKPPETILLIIRRSLGDVLAVSP
                     VVDILARRFASASIDLLVNQDTAAAANLLKGIRNIIAFDDGALKTTPVSSFMMQIGLL
                     QKIFRRYDLAIAFTTNERSNLYAFFAGRTSIGAREKVFAKNWWHRLLLDGGYLIDPQR
                     HILENNLSALSTLGISIDRIVLSATYAGASGQKVQQMLKDFGMDRFIIFHPSGRYRCK
                     SYPEQQRNSLLRLLDSLNIPIIVTGGHSDIDRQVAATLPELKNVRSFIGRLSLEECAA
                     LTDLALAYVGVDTFNAHLAAAFNKPVFVMFGPTIPEIWSPWSNDLQTAGRRNASMQIY
                     GNITLFQSGMACVPCGHSKCRETDRPSPCMEAIDPRNVFHQVQQWFLNLSPESQDNAK
                     NVLPV"
     gene            1246..1917
                     /locus_tag="PLG98_08890"
     CDS             1246..1917
                     /locus_tag="PLG98_08890"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOE33414.1"
                     /translation="MRALLDDPEKYLQQAEARTIQDNFKNKIVYFEIDKKKAVIKIHK
                     YKSAWHRIKRYFRQTRANRSWAYSLLFNAHGIPTPQPIAYKESRIGPLRGDSYFIYEW
                     VEGINGEEYFTKNKANPEIVEKAIDAIVDMTRTIKKLGLIHGDIRLKNMVFRGDDLLL
                     TDFDDTKKRQWYKSNYMNNRDFRGLIEDIYYNVPPEIQPLFLSRLESVGDDIRKLVRS
                     YKHRP"
     gene            2052..3077
                     /locus_tag="PLG98_08895"
     CDS             2052..3077
                     /locus_tag="PLG98_08895"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33415.1"
                     /translation="MTATIAPAKLPVILLYNIDPGWTPEEKDEAIKQSAELGDALRCT
                     GHPTTLVTVIDNDIAEQLRPFDPAKYIVFNWCEELPGIPHSEWKVAKQLESLGYTFTG
                     ADSAALLLAQDKQRVKALLDQCAIPTPAWKIFDAPDPQGWKIFPAIVKPQNEHCSAGI
                     TPESVVLQPTDLQKRITFVLDHYAQPALVEDFIDGREFHVAVWGNETVNVLPAAEMDF
                     SRFGDLNDRLCTYEAKFTPGSLHYEEIKTLLPAPLTTGEKKSLETVCRNAYRASGCRD
                     YARMDIRLRDNIFYVLDVNPNADISSDASMACAAEADGISYAEMGSRIVEMAAKRHPV
                     LGKLFRS"
     gene            3134..4150
                     /locus_tag="PLG98_08900"
     CDS             3134..4150
                     /locus_tag="PLG98_08900"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOE33416.1"
                     /translation="MNKKDFLKTKIEHIDIKKINAVDIVSAMKKMSFSARDLANAADI
                     YDRMLKEKNCAVILTLAGSTSAGGCMQVYVDLVKNNMVDAIVATGASIVDMDFFEALG
                     FRHYQGTPFVDDKLLRKLYIDRIYDTFISENDLQACDKTIKTIADGLAPRPYSSREFI
                     AEMGRYLTKNAKKNNSLVQAAYENNVPVFCPAFSDSSAGFGLVLHQHEHPGNHMSIDS
                     VRDFRELTDIKIKAGTTGLLMIGGGVPKNFAQDTVVCAEILGYDVPMHKYAIQITVAD
                     VRDGACSSSTLKEANSWGKVDSSCEQMVYAEATMALPLLASYAYHKGNWKKRKKWNLS
                     RIFK"
     gene            4485..6974
                     /locus_tag="PLG98_08905"
     CDS             4485..6974
                     /locus_tag="PLG98_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE33417.1"
                     /translation="MEDKNERTKLTERLFSSEDNNLPLLAKDIISNAGVAIYIVHNSQ
                     FVYVSELYQKLTGYAEKELIGEYSLKNIHPDDRDKVRKQAIKCLKKESFEPYEYRYIR
                     KDNEIRYILETVAPIIHKGERATLGSFMDITERKIIEEKYRNILENIQEGYFEVDLEG
                     NFTFFNNAVSRVLGYSREELMGMNNRQYTPDKDSARKVFQAYHHVYETGEPLNAVGWH
                     IITKQGAKRYIEGSISLLKDSQGKPIGFRGITHDVTERKVAEEALRQSEEKYRNILEN
                     IQEAYFEVDLAGNFIFFNDSLCRITGGTREELMGANYTRFSDPATSKNVFLAFNKIFK
                     TGEATEGFDWLIIRKDGSKKYIEASASLNKDASGKPIGFRGVIRDITERKRIEQELNH
                     MATHDALTGLPNRLMFSQLLDHAIQSAQRNKKHLAVFFIDLDRFKIINDSLGHEAGDT
                     LLKEMALRFKQSLRTVDIIGRLGGDEFTILVEDFNDLKQVEFLAKKILNTVMQPIALM
                     GEECRVTASIGISVFPKDGLDEQSLMKNADIAMYFAKSEGKNNFQFYSENIRSMSNER
                     LTIETNLRHAMERNEFYLDYQAKLDLQTRAITGVEALLRWNNPYLGLVAPTQFIPIAE
                     ETGMIIPIGRWVIKTACMQNVAWQKQGLPPVCMSVNLSLRQLMYENFAEDVKTILNET
                     RMASELLELEITESTVMHNPKRLVPILSKLKKMGIRMAIDDFGTGYSSLAQIKNFPIN
                     TLKVDRSFIRNLPQDTDNKAITEAVIFMGKSLKLKVVAEGVETREQAKFLKDQICDEM
                     QGFYFSKPIGPDGFADLLRENSVSGTDPGRQ"
     gene            complement(7020..7544)
                     /locus_tag="PLG98_08910"
     CDS             complement(7020..7544)
                     /locus_tag="PLG98_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOE33418.1"
                     /translation="MALGIFEDEKPPRGIGGLIDWRLNGMLSREIKNGRIGGGFKEKV
                     VIPFPERIGSEILLLFGLGRVPDITYDRIYDVAYTVTQIIDSMAIKSFAFDLYGEGRA
                     NLVTSDIVEAAVTGIFDFLASDIEKLSEMNACAVTSPSNLHSVAQGVRQFKNNVNDKG
                     SVDISPLEECLAKN"
     gene            complement(7549..8004)
                     /gene="nusB"
                     /locus_tag="PLG98_08915"
     CDS             complement(7549..8004)
                     /gene="nusB"
                     /locus_tag="PLG98_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOE33419.1"
                     /translation="MCGRRKAREIALQVLYSLNFVDIPAQEALDLFWGNFVAPKSAKD
                     FAAFLVMGTCDHRKELDQLIAGCSDNWSLGRMSRVDINILRLAVFELLYCDDIPPKVT
                     MNEAIDLGKIFGSENSGSFINGILDALNLKLNKKNAAKSLDRKPRSSKT"
     gene            complement(8081..8545)
                     /gene="ribE"
                     /locus_tag="PLG98_08920"
     CDS             complement(8081..8545)
                     /gene="ribE"
                     /locus_tag="PLG98_08920"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOE33420.1"
                     /translation="MLKIIEGKTVAKGMKFGIVASRFNDFICGRLIEGTIDALIRAGA
                     EEKDIVIYKVPGSFELPLTAKKLAKSGRFDAVICLGAIIRGATPHFEYISAEVSKGIA
                     VVGLETEVPVVFGVLTTDTIEQAIERAGTKSGNKGADAAMTAIEMVDLFKKI"
     gene            complement(8593..9795)
                     /locus_tag="PLG98_08925"
     CDS             complement(8593..9795)
                     /locus_tag="PLG98_08925"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOE33421.1"
                     /translation="MAISTIQEAIEDIKNGKMIILVDDEDRENEGDLCMAAQFATAKT
                     INFMARYGRGLICLTLNEDMADKLHLKQMVQDNQCRFGTAFTISIEARHGVTTGISAA
                     DRATTIQAAVNPEAKPDDLVSPGHVFPIRAKKGGVLVRTGQTEGSVDLCRLAGLTPAG
                     VICEVMKDDGTMARMPDLEIFAKEHKLKIVTIADLIDYRMQNESLIKRMAEATLPTSF
                     GGDFKMIVYENEVDDWQHIALVKGDIKEDDEVLVRVHSECLTGDLFGSLRCDCGDQLH
                     RAMNMVEKEGKGIIVYMRQEGRGIGLVNKIKAYALQEQGKDTVEANIELGFKPDLRDY
                     GIGAQILADLGVRKMRLLTNNPKKIVGLEGYGIEVVKRVPIEIDPNENNIHYMKTKKK
                     KMGHLLKI"
     gene            complement(9814..>10070)
                     /locus_tag="PLG98_08930"
     CDS             complement(9814..>10070)
                     /locus_tag="PLG98_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOE33422.1"
                     /translation="DTISRYIVEKGSVAIDGISLTVNKLEKSRFYVNIIPHTAVNTTL
                     VTRKEGEWVNIETDILGRYVENLLQSNKGIDKDFLVKHGF"
     assembly_gap    10071..10170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10270..10731)
                     /gene="nrdR"
                     /locus_tag="PLG98_08935"
     CDS             complement(10270..10731)
                     /gene="nrdR"
                     /locus_tag="PLG98_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOE33423.1"
                     /translation="MKCPFCGHTENKVIDSRISKDGKAVRRRRECLGCTKRFTTYEYV
                     EDVLPMVVKKDGRREQFDRQKILTGIKKACEKRPISMEAIDKLVENIEQACQEMQAEE
                     ISSTVIGEKIMSELKNFDGVAYVRFASVYRQFRDVGEFMSELKDLLSKGKK"
     gene            complement(10752..11237)
                     /locus_tag="PLG98_08940"
     CDS             complement(10752..11237)
                     /locus_tag="PLG98_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOE33424.1"
                     /translation="MMEIKSSAAVLREKKKKRPDWDSYFLDIVDLVSRRSTCCRRSVG
                     AGLVRDSRILATGYNGAPSKLQHCLDIGCLREQLKVPSGERHELCRGLHAEQNAIIQA
                     ALHGVSTKGSTLYCTNHPCVICAKMIINAGIIRVVIRDGYHDQLAAEMLKEAGIRVEQ
                     L"
     gene            complement(11271..>11768)
                     /gene="glyA"
                     /locus_tag="PLG98_08945"
     CDS             complement(11271..>11768)
                     /gene="glyA"
                     /locus_tag="PLG98_08945"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416100.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOE33425.1"
                     /translation="PGMQGGPLMHVIAAKAVAFKEALQPAFKEYQAQIIKNARAMADE
                     LKNQGFRLVSGGTDNHLMLVDLTDKNVTGKDVQDALDRASITVNKNGIPFDTKGPQVT
                     SGIRIGTPAVTTRGMKEDEMRLIASCIADVIKNISSEQKITSVAQKIKVLCDRFPLYQ
                     GRLKN"
BASE COUNT         3065 a         2984 c         2675 g         2944 t
ORIGIN      
        1 cagcagaaac aaagaccgtg ccgaaacgct catcaacagc gaagtggcgg ccatcccgtg
       61 atgtcatgaa accacctgaa actatcctgt tgatcatcag aagatccctt ggcgatgtcc
      121 tggccgtctc tccggtggtc gatatccttg cacgccgatt cgcctcggcc tccatcgacc
      181 tgctggtcaa ccaagacacg gcggcggcgg caaacctgtt gaagggcatc cggaacatca
      241 ttgcgtttga tgacggcgcg ctgaagacaa cccctgtgtc ctctttcatg atgcaaatcg
      301 gattattgca aaaaatattc cgacgttatg atctagccat tgccttcacg acaaacgaac
      361 gaagtaatct atacgcattt ttcgccgggc ggacatcgat cggcgcaagg gagaaggttt
      421 ttgcaaaaaa ctggtggcac cgtttgcttc ttgacggcgg ctatctgatc gatccgcaac
      481 gtcatattct ggaaaataat ctttccgccc tgagcaccct cggcatctct atcgacagaa
      541 tcgtgttgtc cgccacatac gccggggcgt ccggacaaaa agtgcagcag atgttgaaag
      601 atttcggcat ggaccgtttc atcatttttc atccgtcggg gcgttaccgg tgcaaatcct
      661 atcctgaaca acagagaaac agccttttgc ggttgcttga ctcgttgaat attccgatta
      721 tcgtcacagg cggacacagc gacatcgacc ggcaggttgc cgccacgtta ccggaattga
      781 aaaatgtgcg gtcattcatc ggcagactgt cgcttgagga atgtgccgct ttaaccgacc
      841 tggcgctggc ttatgtcggg gtcgacacct tcaacgctca tcttgccgca gccttcaaca
      901 aaccggtttt tgtcatgttc ggcccgacga ttccggaaat ctggtcgccc tggtcaaacg
      961 atctgcagac agcggggcgc cggaatgcat cgatgcagat ttatggaaac atcactcttt
     1021 ttcaatccgg catggcgtgt gttccctgcg gacactccaa atgcagagag acggaccggc
     1081 catcgccgtg catggaggcc atcgatcccc gcaatgtttt ccatcaggtc caacaatggt
     1141 ttttaaattt gtctccggaa agtcaggaca atgcaaaaaa tgttttaccg gtttaaaaat
     1201 ttttctacgt gcgttgtgtg cgatcggtct tttctatcgg acgggatgag ggcgctcctg
     1261 gacgatccgg agaaatatct gcagcaggcc gaagccagaa ccatccaaga caacttcaaa
     1321 aacaaaatcg tttattttga gatcgacaag aaaaaagccg tcattaaaat tcataaatac
     1381 aaatccgcct ggcacaggat caaacgctat ttcaggcaaa cgcgggcgaa cagatcatgg
     1441 gcatactctt tgctgttcaa cgcgcacggg atacctacgc cgcaaccgat tgcctataaa
     1501 gaatccagaa tcgggccttt gcgcggcgat tcctatttca tctatgaatg ggtcgaaggc
     1561 atcaacggtg aagaatattt cacgaaaaac aaggccaatc cggaaattgt cgaaaaagcc
     1621 atagacgcca tcgtggacat gacccgaacg attaaaaaac tcggtctcat ccacggagac
     1681 attcgtttaa aaaatatggt tttcagagga gacgaccttc tgttaaccga ttttgatgat
     1741 acgaaaaaga ggcagtggta caagagcaat tacatgaata atcgcgattt cagagggctg
     1801 attgaagaca tttattacaa tgtgcctccg gaaattcaac cgcttttttt atcccgcctg
     1861 gaatctgtcg gcgacgatat ccgaaaactt gtccggtcat acaaacatcg tccttaaggg
     1921 ccgagtccgc aagagagcca atcttctctt gacttgtttc catggcttct ctacaatctt
     1981 tttcggatga agcatattga tcagatttca ttccaagaaa atcagattga aaaaatcata
     2041 aggaacaaca cgtgaccgca accatagcac ccgcaaaact tccggtgatc ctcttgtata
     2101 atatcgatcc gggttggacg ccggaagaga aagatgaagc aatcaaacaa tcggccgaac
     2161 tgggagatgc cctccgctgc acaggccacc cgacgaccct ggttaccgtg attgataacg
     2221 acattgccga gcagcttcgt cccttcgatc cggcaaaata catcgtgttc aactggtgcg
     2281 aggaacttcc gggcatcccg cacagcgaat ggaaagtggc caaacaactg gagtcgctgg
     2341 gttatacgtt taccggtgcc gattcggccg cgctgttgct ggcgcaggac aaacagcggg
     2401 tgaaggccct gctggatcag tgcgccattc ccacgcctgc atggaaaatc ttcgatgcgc
     2461 ctgaccctca gggctggaaa atttttccgg caatcgtcaa acctcaaaac gaacattgtt
     2521 ccgcaggcat cacgcccgag tctgttgttc tgcaaccgac ggatttgcaa aaacgcatca
     2581 cctttgtgct cgatcattat gcgcaaccgg cgctggtgga agactttatt gacggacggg
     2641 agtttcatgt tgccgtatgg ggaaacgaaa ccgtcaacgt cctgcccgcc gcagagatgg
     2701 acttttcccg cttcggagac ctcaatgacc ggctctgcac ctacgaggca aaatttactc
     2761 ccgggtcgct gcattatgaa gagatcaaaa cccttctgcc cgccccactg accacgggtg
     2821 aaaaaaagtc gctggaaacg gtttgccgca acgcctatcg ggcatccgga tgccgggatt
     2881 atgcccgcat ggatatacgc ttgcgggaca atatttttta cgttctggat gtcaatccca
     2941 atgcagatat cagttccgat gccagcatgg cctgcgctgc ggaagcggat ggaatcagtt
     3001 atgccgaaat gggcagccgc atcgttgaga tggccgcaaa aaggcatccg gtccttggga
     3061 aactcttccg gagttgactt ttaacaggcg atggtttaga catacgcata ttcattaagg
     3121 gagtcgcctt gtcatgaaca aaaaagattt tctcaaaacc aagatcgaac acatcgatat
     3181 taaaaaaata aacgcggtgg acatcgtcag cgcgatgaaa aaaatgtcct tttccgcccg
     3241 cgatctggcc aatgccgccg atatatacga ccgtatgttg aaagaaaaaa actgcgcggt
     3301 gatcctgaca ctggccggtt cgacatccgc cggcggctgc atgcaggttt atgtcgattt
     3361 ggtgaaaaac aatatggtgg atgccattgt ggccaccggc gcatccattg tcgacatgga
     3421 tttttttgaa gcactgggat tcagacatta tcagggcaca ccgtttgtgg acgacaaact
     3481 gttgcgcaag ctctacatcg accggattta cgacacgttt attagcgaaa acgatttgca
     3541 ggcctgcgac aagaccatta aaacaatcgc ggatggcctt gcgccgcggc cttattcttc
     3601 ccgcgaattc attgccgaga tgggcaggta cctcacaaaa aacgccaaaa agaacaattc
     3661 tctggtccag gcggcttatg aaaacaacgt gccggtcttc tgcccggcat tttcggacag
     3721 ttccgccggt ttcggcctgg tcctgcatca gcatgaacat ccgggaaacc atatgtcgat
     3781 tgattccgtc agggacttcc gggaactgac cgatattaaa atcaaagcgg gaacaaccgg
     3841 cttattgatg atcggcggcg gtgtcccgaa gaactttgct caggataccg tggtttgcgc
     3901 ggaaatactg ggctacgatg tccccatgca caaatatgcc attcagatta ccgtggcgga
     3961 tgtgcgcgat ggtgcctgtt caagctcaac gctcaaggaa gccaattcat ggggcaaggt
     4021 cgattcgtcg tgcgaacaga tggtttacgc cgaagcgaca atggctctgc ccttgctggc
     4081 cagttatgcg taccacaaag gcaactggaa gaaacggaaa aaatggaatt tatccaggat
     4141 tttcaaataa atatctgcct gctgtattca atagtttttt aatgcattgg taacttttac
     4201 gggcggaata accgtcatta tggggaaaat aaccgttttt tctcaaaacg atttagggcc
     4261 ccgtacggtc agacagtctc cgttaagcca ttagttatta attttattaa atattatctt
     4321 aaaacttgcc gcgctgccct atcactcatt taaggcgaca ttgggcacat aaattgctta
     4381 aagtcataga atatgcatca gcttttcttc atgaatacgc gattggcgtg atcaattgca
     4441 ttggtcagct catcaccgaa accggcaacg taacttgaat aacgatggaa gacaaaaacg
     4501 aacgaacaaa attaacagaa agattgtttt cttctgaaga caacaatctt ccattactgg
     4561 caaaagatat tatatcgaat gccggcgtag caatttatat cgttcacaac agtcaatttg
     4621 tttacgtcag cgaattatac cagaaactca ccggctacgc ggaaaaagaa cttatcggcg
     4681 aatattccct caaaaatatt catcctgatg acagggataa agtcaggaaa caggccatta
     4741 aatgcctgaa aaaagaaagt tttgagccct acgaatacag atatatccgc aaagataatg
     4801 aaatcagata cattctggaa acagtggcgc cgattatcca taaaggcgag cgggcgacac
     4861 tgggcagttt tatggatatc acggaacgca agatcatcga agaaaaatac agaaacattc
     4921 tggaaaatat tcaggaaggt tattttgaag ttgatcttga ggggaatttt acttttttta
     4981 ataatgccgt ctcccgtgtt cttggatatt cccgggaaga attgatgggc atgaataacc
     5041 ggcagtacac acccgacaag gactccgcgc gaaaagtttt tcaggcttat catcatgttt
     5101 acgaaacagg tgagccgctg aatgcagtcg gctggcacat cattaccaag caaggcgcca
     5161 aaagatatat tgaaggatcc atttcgttac tcaaggattc tcaaggcaaa ccaatcggat
     5221 ttcgcggtat cacccacgat gtcaccgaac gtaaagtggc cgaagaagcg ctgcgtcaaa
     5281 gcgaagaaaa gtacagaaac attctggaaa acattcagga ggcctatttt gaagttgatc
     5341 ttgcaggcaa ttttatcttc tttaacgatt cgctgtgtcg catcacagga ggaacaagag
     5401 aagaattaat gggtgccaat tacacgcggt tttccgatcc ggcaacgtca aaaaatgtgt
     5461 ttctggcatt caataaaatt ttcaaaacag gagaggccac ggagggattc gactggctga
     5521 ttatcagaaa agatggttcc aaaaaatata ttgaagcgtc cgcatcctta aataaagatg
     5581 cttcgggaaa gcccataggg ttccggggag tcatccgcga tattaccgaa cgcaagcgca
     5641 ttgaacagga actcaatcac atggccacgc acgacgcgct caccggcctg ccgaaccgcc
     5701 tgatgttcag ccagttgctg gatcacgcca tccaatccgc gcaacgcaac aaaaaacacc
     5761 tcgctgtttt cttcatcgat ctggatcgct tcaaaatcat taatgattcc ctcggacacg
     5821 aagccggaga cactctgctc aaagagatgg ctttgcggtt caaacagtct ctgcgtaccg
     5881 tcgatattat cggacgtctc ggaggcgacg aattcacgat tctggttgaa gattttaacg
     5941 atctgaagca ggtggaattt ctggccaaga agattttgaa caccgtcatg caaccgattg
     6001 cgctcatggg ggaagaatgt cgtgtcaccg ccagtatcgg tatcagcgtc ttcccgaagg
     6061 acggactgga tgaacagtcc ttaatgaaaa atgcggacat tgccatgtac tttgccaaat
     6121 cggaaggaaa aaacaatttc cagttttact ccgaaaatat ccgctccatg tccaacgaac
     6181 ggctgaccat cgaaaccaac ctgcgtcatg caatggaacg caatgaattt tacctcgatt
     6241 accaggcaaa actggatctg cagaccaggg caatcaccgg cgtcgaggcg cttctgcgct
     6301 ggaataatcc gtatctggga ctggtcgcgc cgacgcagtt tattccgatt gcggaggaga
     6361 caggcatgat tatacccatc ggccgatggg tgatcaaaac cgcctgcatg cagaacgtcg
     6421 cctggcagaa gcagggactg ccgcccgtct gcatgtcggt caacctgtcg cttcgtcaac
     6481 tcatgtatga aaactttgca gaagatgtca aaaccattct gaatgaaacg cgcatggctt
     6541 cggaactact ggagctggaa atcaccgaaa gcacggtcat gcacaacccc aaacgtcttg
     6601 tcccgatcct gtcaaaatta aagaaaatgg gaatacggat ggcaattgat gatttcggca
     6661 cgggttactc ttcgcttgca cagatcaaaa acttcccgat caacacgctg aaagtagacc
     6721 gatcatttat ccgcaatctt ccgcaggata ccgataataa agccatcacg gaagcggtga
     6781 tttttatggg caaatcgttg aaactcaaag ttgtcgccga aggcgtggaa accagggaac
     6841 aggcgaaatt tcttaaagat cagatctgcg acgaaatgca gggcttctat ttcagcaaac
     6901 ccatcgggcc tgacggcttt gccgatcttc ttcgcgaaaa ttctgtttcg ggaacagatc
     6961 ccggccgtca ataatttttt tgtaaaaaac aaccggcatt attgtgtcat ctgcaaaaat
     7021 cagtttttcg ccagacattc ttccagcggg gaaatatcca cggatccctt gtcgttgaca
     7081 ttgtttttga attgtctgac gccttgagcg accgaatgaa gattcgaagg tgaggtgact
     7141 gcacaggcgt tcatttccga gagtttttcg atatcggatg ccagaaaatc aaaaattccg
     7201 gtcaccgccg cctccacaat atcggacgtg acgagatttg cgcgtccttc tccgtacaga
     7261 tcaaacgcaa agctttttat ggccatgctg tcgataatct gagttacggt atatgcgacg
     7321 tcataaattc tatcatacgt gatatcggga acacgaccaa gtccgaataa taacaggatt
     7381 tccgaaccga tcctttccgg aaaaggaata acaacttttt ccttaaaacc gccgccgatt
     7441 cttccatttt tgatttcccg ggaaagcatg ccattgagac gccagtcgat caaaccgccg
     7501 atgccgcgcg gcggcttttc gtcttcaaag atgccgagcg ccaaacattt atgttttgga
     7561 agatcggggt tttctatcga gagacttagc tgcatttttt ttatttaatt tcagattgag
     7621 agcgtcgagt atgccgttga tgaacgatcc ggagttttcc gacccgaaga tttttcccag
     7681 atcaatggcc tcattcatcg tgaccttcgg aggaatatca tcacagtaga gcaattcaaa
     7741 tacggcaaga cgtaatatgt tgatatccac gcgggacatt cgccccaacg accagttatc
     7801 cgaacatccg gcaatgagtt gatccagttc tttcctgtgg tcgcatgttc ccatcacgag
     7861 aaaggcggca aaatccttgg cagattttgg tgcaacaaaa ttaccccaga aaagatccag
     7921 ggcttcctgc gccggaatat cgacaaaatt aagactgtaa agaacctgta aggcaatttc
     7981 tcttgccttt cgccgcccgc acatcaattt tcaacctcaa agaaccagaa catccgatca
     8041 tttctgtact gttgccgtca gaaaaaacgg cattgcaaca tcaaattttt ttgaacaaat
     8101 ccaccatctc aatggctgtc atcgctgcgt cggccccttt attgcccgac ttcgttcccg
     8161 cacgttcgat agcctgttca atggtgtccg ttgtcaacac accgaaaaca acgggcactt
     8221 ccgtttcaag gccgacaacg gcaatcccct tggagacttc ggcactgata tattcgaaat
     8281 gcggcgttgc accgcggata atggccccca gacagatgac ggcatcaaat cgaccgctct
     8341 tggccagttt ctttgccgtc agcggcagtt caaaagagcc gggaacttta taaatgacga
     8401 tatccttttc ttctgcaccg gccctgatca gcgcgtcgat agtcccttcg attaatctgc
     8461 cgcatataaa atcattgaaa cggctggcca cgatgccaaa cttcattccc tttgcaacgg
     8521 tctttccttc aattattttc agcatagttc ttaaccatcc ttacgtttta ttttagcctt
     8581 gcggtgtaaa atctatattt tcaaaaggtg ccccatcttt tttttctttg ttttcatgta
     8641 gtgaatgttg ttttcattgg gatcgatttc gatgggaacg cgtttgacca cttcgatacc
     8701 gtatccttcc agaccgacaa tcttcttcgg attgtttgtc aggagacgca ttttgcgcac
     8761 gcccagatca gccaaaatct gagcaccgat gccataatcg cgtaaatccg gtttgaatcc
     8821 gagttcgata ttggcctcca cggtatcctt gccctgttcc tgcagagcat aagctttgat
     8881 cttgttgacc agtccgatgc cgcggccttc ctggcgcata taaacgataa tgcccttgcc
     8941 ttctttttcc accatgttca tcgcacgatg aagctggtcg ccgcagtcgc agcgcaacga
     9001 cccgaaaaga tctccggtca ggcattcgga atgaacccgg accaggactt cgtcatcttc
     9061 tttaatatcg cctttcacca gggcgatatg ctgccagtcg tcaacttcat tttcataaac
     9121 gatcatttta aaatcgccgc cgaaactggt cggcaaggtc gcttccgcca tacgtttgat
     9181 aagcgattca ttctgcatcc ggtaatcgat aaggtcggca attgtcacta tttttaactt
     9241 gtgttccttg gcaaatattt ccaaatccgg catcctagcc atggtgccgt catccttcat
     9301 aacttcacag atcacgccgg ccggtgtcaa accggcaagt cggcataaat ccaccgaccc
     9361 ttccgtctgc cccgtccgga ccagaacccc cccttttttg gcacggatag gaaagacatg
     9421 tccgggactg accaaatcat ccggtttggc ctccggattc acagccgcct gaatcgttgt
     9481 tgcccgatcc gctgcggata taccggtggt gacgccatgg cgcgcttcga tggaaatggt
     9541 aaaagcggtg ccgaaacggc actgattatc ctgaaccatt tgtttcagat gaagcttatc
     9601 cgccatatct tcattaaggg tcagacaaat caaaccgcgg ccgtagcggg ccataaagtt
     9661 gatggtttta gccgtggcaa attgcgctgc catgcaaaga tccccttcgt tttcacgatc
     9721 ttcatcatca acaagaatga tcattttccc gtttttgata tcttctattg cttcctgtat
     9781 tgtgctgatt gccattcgtc tttcctttat tccttaaaaa ccgtgtttca caaggaaatc
     9841 cttatcgatc cccttatttg attgcaataa gttttccacg taccggccta aaatatccgt
     9901 ttcaatattc acccactccc cttctttgcg ggttaccagg gtggtattga ccgccgtgtg
     9961 cgggataata ttaacataaa accggctttt ttcaagtttg ttcaccgtta gactgatgcc
    10021 gtcaatcgcc accgaacctt tttccacaat gtagcgggaa atcgtatcat nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccagactgat gccggcgtca atgtccagaa
    10201 ccgcatctgc gcccttcatg gtcaaacgcc tcaccttgcc cagtccttct acgattccgg
    10261 taaacatgat tactttttac ctttgcttaa aagatccttc agctccgaca tgaactcacc
    10321 gacatcgcga aactgccggt aaacggacgc aaatcggaca taggcgacgc cgtcgaaatt
    10381 tttcaactcg ctcatgatct tttctccaat gaccgtggac gaaatttctt cggcctgcat
    10441 ttcctgacag gcctgttcaa tgttttcgac aagtttgtca atcgcctcca tgctgatcgg
    10501 tcgtttctcg caggcttttt tgatgccggt cagaattttc tgccggtcaa actgctcgcg
    10561 gcgaccgtct ttcttcacca ccatgggcag aacgtcttcc acgtattcat aggtagtgaa
    10621 acgcttggtg caccccaggc attcccggcg ccggcgaacg gctttcccgt ccttgctgat
    10681 tcgagaatca atcaccttat tttccgtgtg accgcaaaaa gggcatttca tgatgatgtc
    10741 accttatgtg gttaaagttg ctctacgcgg atccccgctt ctttgagcat ctccgcagcc
    10801 agttgatcat gataaccgtc acggatgacg acacggatga tcccggcatt aatgatcatt
    10861 ttggcacaaa tgacacaggg gtgattcgtg caatacagcg tcgatccttt cgtgctgacg
    10921 ccgtgcagcg ccgcctgaat aatcgcattc tgctccgcat gcagcccgcg gcataattcg
    10981 tgacgttcgc cggaaggaac cttcaactgt tcccgaagac agccgatatc gagacagtgc
    11041 tgaagcttgg agggagcgcc gttgtatccg gtggccagaa ttcggctgtc ccgaaccagt
    11101 ccggccccta ccgatctccg gcaacaggta ctgcgcctgg aaacaaggtc gacaatatcc
    11161 agaaaataac tgtcccagtc cggtcgtttt tttttcttct cccgcaaaac ggctgctgac
    11221 gattttattt ccatcataat cgctttcgat caaaaaaaat ggtccgtgtt ttaatttttc
    11281 agccgcccct gatacagcgg aaaacgatcg caaaggactt tgatcttctg tgcaaccgat
    11341 gtgattttct gctcgctact gatgttttta atcacgtcag caatacacga cgcaattaaa
    11401 cgcatttcat cttccttcat gcctcttgtg gtcacggcgg gcgtgccgat tctgataccg
    11461 ctggtcacct gaggcccctt cgtatcaaag ggaatgccgt tcttattgac ggtgatggag
    11521 gcgcgatcca gagcgtcctg cacgtccttt ccggtgacat tcttatccgt caagtcgacc
    11581 agcatgagat gattgtcggt gccgccggac accagccgga atccctgatt cttcagttca
    11641 tccgccatcg cccgagcgtt tttaatgatc tgtgcctgat attccttgaa tgccggctga
    11701 agcgcttcct tgaaggcaac cgccttggcg gcaataacat gcatcaacgg gccgccctgc
    11761 atgcccgg
//
LOCUS       DAOSME010000062        11717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6325_length_11717_cov_19.452495, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000062 DAOSME010000000
VERSION     DAOSME010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6325_length_11717_cov_19.452495"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="PLG98_08950"
     CDS             complement(<1..1711)
                     /locus_tag="PLG98_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE33426.1"
                     /translation="MEQQNRQSSENEEQYGSLLEAIEEGYYEVDLAGNFTFFNKAMCR
                     ILGYAPDEMRGMNNRQFTDKENAKKLFETFNRVYKTGESTKAFDWQLIRKDGNKKYIE
                     TSVSLIKDASGKPTGFKGIVHDITERKQAEEALRESEALYRLLADNISEHVWVMDLNL
                     NLIYISPSVEKIYGYALDEISQIKMRQLFTADSYRKVVETFMKELEKAMNSKPPPTGT
                     NYIMELEAYHKDGHKIWIENRLHFIRDKNGKPVSLLGETRDITDRKLAQEALKASEEK
                     YRLLADHMKDQVWMMDMNMQITYVSPSVERITGYSFEEIKILPLDQLLTPESVRKAME
                     FITNRMPKAFNLASLDSQYRTLELEFRTKEGKTIWGECSFNFIRNENNEPISILGEAR
                     NITDRKIAEDKLRQSEEKYRTILEDIKEGYFETDLFGNFTFFNDTVCRVLGYSRDELT
                     GMSNKIYTDAEERKKVYRAFHQVYMTGEPYRDLEWQIRGKDGTRKYIEGFISLLRDSS
                     GNPKGFRGIAHDITERKIADEKLQKTLESLKKAVGTTIQVLISALEARDPYTAGHQSR
                     TANL"
     gene            complement(1997..2197)
                     /locus_tag="PLG98_08955"
     CDS             complement(1997..2197)
                     /locus_tag="PLG98_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33427.1"
                     /translation="MTKNEISREEIIKKLVDSDLDGLSQEKSIKFLVRILHNGWKGYN
                     EMSDTELLEAFNHREFRKTVRT"
     gene            complement(2360..3592)
                     /locus_tag="PLG98_08960"
     CDS             complement(2360..3592)
                     /locus_tag="PLG98_08960"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOE33428.1"
                     /translation="MEDIKITPADVQETLCFTGNIIEESGARLAGSESCKKAAVLIMN
                     EMNKHCDSVSMEEFDIHPKAFLGFFKVVVVIYILSSFLLYFDYVVAGAAGYLLGAFIM
                     LGESIFYWEMLDPFYRKMKGYNVIGTIEPEGEVKQQIILSGHHDSAHEFRFLAHHQKL
                     YAVRIMTAVIIIFAALVLSWIWVYHRSYSGADPAFVPYLRYGVFAGLIFVAPMYFFIS
                     EKGTPGAGDNMIAVAAIIKLAEIFNRAKQNGAGLLKNTRLMFLSADAEEAGLRGARAF
                     VKRHRGALLKMPTYHFNIDSIYNLGELQFLTRDINSVVRLSKNMARECKAVADQLGYR
                     TRLFAITPGGGATDAAEFAKAGVEATTLIGMPTNLIRDGMVYHTLQDNVEHIEPEAVE
                     ATLKIAFVYILNKERDNI"
     gene            complement(3818..4399)
                     /locus_tag="PLG98_08965"
     CDS             complement(3818..4399)
                     /locus_tag="PLG98_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33429.1"
                     /translation="MKNKVYKALLLTLIVLMPVSAMADVRVHVNIPLPLPVVFPSPPH
                     LVVIPDTDVYAVADIGEDIFFHAGWWWRPWNNRWYRSRYYDRDWAYHPYQPSFQRYVP
                     ADWRQNYRHKTWNGYRWEPQRRSYQDIQRNWHVWEKNRHWDKQSYGVKKITKRAPAPQ
                     YRSNTRPAMTKPAMQHNVKSRGDFRTSERRDRR"
     gene            complement(4478..4963)
                     /locus_tag="PLG98_08970"
     CDS             complement(4478..4963)
                     /locus_tag="PLG98_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOE33430.1"
                     /translation="MKKITFTMIAVFLGLLLTSQAFAWGPGPGKKSGYCRGVVLEELN
                     VTEDQKTKLDALQSQHFKAVHPLREKIRDKSAALRKLWLQVDPDKNKIMAKQKEVRVL
                     RDQLEDNKTAYRFEVNKILTPKQKEKLAKCGWNKKSGFGPRGGKRGEQWNGHGNCLYL
                     P"
     gene            complement(5530..>6015)
                     /locus_tag="PLG98_08975"
     CDS             complement(5530..>6015)
                     /locus_tag="PLG98_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33431.1"
                     /translation="AMGETSTAADWFNRADGLWDGKKFIEVKEAMAYLDKAIEQQPNY
                     ANAYISRGMAYNQTGDYQRAIQDYNLALLFTNDENSLTKIFDKMGVAYANLGQYQRAI
                     NNFNHAISLKPDYADAYNNRGIVYFLQGDKKQCCNDAQKACELKNCELLSWARERGAC
                     P"
     assembly_gap    6016..6115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6116..6679)
                     /locus_tag="PLG98_08980"
     CDS             complement(<6116..6679)
                     /locus_tag="PLG98_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33432.1"
                     /translation="MAMKVLNNRNFIFILAMILGLFLPQPAEWTKVLMIPALALVMTL
                     ATVNVPNNYFLNPRAILKPSLAGMFMSYIILAGVLLCLSALLIEDKNLWIGFVLIASV
                     PPAVAVIPFTAILEGNVDYTLSGTVASYIAALLIMPVILWIFIGTVFEDPYKLLQIML
                     LLIVLPLILSRLILYFNWQEKLSSVRGF"
     gene            complement(7066..7281)
                     /locus_tag="PLG98_08985"
     CDS             complement(7066..7281)
                     /locus_tag="PLG98_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33433.1"
                     /translation="MNEEIDDDLIDIELEGDALEKGMRKLESEAERLLHYTNDLLQEL
                     NKERAKREAMKQEDEKTQSTEEGSASS"
     gene            complement(7464..7898)
                     /locus_tag="PLG98_08990"
     CDS             complement(7464..7898)
                     /locus_tag="PLG98_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33434.1"
                     /translation="MQKRNDRQPYSLESIVELYPRLYSQKRLDEWMDLFDKRAIMVRV
                     QKGNPVSFVSITEGITEQREYAAENALFDEKWHHVKINRHGNIAVIKANYILTAEREI
                     RKGIDVLTLCCRDGGWRIICLAYEQKEMVRRSIPRKDSVKTS"
     gene            complement(7964..8464)
                     /locus_tag="PLG98_08995"
     CDS             complement(7964..8464)
                     /locus_tag="PLG98_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33435.1"
                     /translation="MVKRKYLAMGLILVSAFAVYFLFFVDWEARAVKKHLQALAKELE
                     WSTGENELAVAHRIRRIQEGMAENCQVDIPSYGISRAVVRHDVPVYLMTARGYYKNLS
                     VELEDLEIETLELPAAQAVATAYVKAIQADGVLNDEVLVIRFRLQKVNKKWQVNEAGE
                     VHVLEK"
     gene            complement(8458..8955)
                     /gene="lepB"
                     /locus_tag="PLG98_09000"
     CDS             complement(8458..8955)
                     /gene="lepB"
                     /locus_tag="PLG98_09000"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOE33436.1"
                     /translation="MNKTAIRRFFFPDITARYVLRLAAVAAVAFILFKYVFIPFRIQG
                     ESMAPTYVTGSFNFCFALRYMFASPAPPDVVTVRMAGQRIMLLKRVVAVSGQRVEFSK
                     GILFVDDKKINEPYVKKKSNWNLEPIIVKPDHVYVVGDNRAMPAEAHTFGQAPVSRIV
                     GVPLW"
     gene            complement(8993..9163)
                     /locus_tag="PLG98_09005"
     CDS             complement(8993..9163)
                     /locus_tag="PLG98_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33437.1"
                     /translation="MDKVANEIGHYIIRAFDAMGAEIIGDVFRWSGVTETGYGAIFIV
                     LAILVVIYIVKK"
     gene            complement(9257..10579)
                     /locus_tag="PLG98_09010"
     CDS             complement(9257..10579)
                     /locus_tag="PLG98_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOE33438.1"
                     /translation="MKNYKILIIGSGPAGLFAALRLEKLGMDDIAVIDRNPYPAGGLL
                     NDGKLNFDYRVGLDIDELQIDRVTAEGMMLEIKRIFTGFPRCRQVTFTDNRNNLRALA
                     DMAAEHGAQFIAPEQWHWGTDNGRALVDYLRGQLKKTDFLLSTEVESVEKKREGSFRL
                     LCRIDKKRKSCCAPVILAAPGRSGAYWFRDIARALDIRNNFGPIDVGIRLELNRKFYD
                     FVTGIVYDPKFIFTTGRHGDKVRTFCTNPGGRVRVESYGDFKLVNGDALSGKKTKNTN
                     FALINTVALTKPYSDTTEFGLSIAKQFFLLGGGKPIVQRIGDFREGRRSSLSTFNSTA
                     RHFEVCRATCSATPGDITLAMPARIMDNLWESLKSLDKIIPGILHPSTLMYAPEIKFF
                     DTHFPTDHYLETNVKGIFVAGDGTGKSRGIVGAAMSGMIAAEGIMNGR"
     gene            10939..11385
                     /locus_tag="PLG98_09015"
     CDS             10939..11385
                     /locus_tag="PLG98_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE33439.1"
                     /translation="MFAPKRILVPTDFSDYSDEALKQALELAKQYHSKVYLLHVANPV
                     TQCTADYCLDVDTFKKAEESTMIKARDMMQQELQKFPESQEIEIISDIREGEPVAEIL
                     KEQEEKGVDLIVMPSHGRSGLLKRLMGQIAEKVMEATKTPVLLVHH"
     gene            complement(11451..>11717)
                     /locus_tag="PLG98_09020"
     CDS             complement(11451..>11717)
                     /locus_tag="PLG98_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33440.1"
                     /translation="TRLIRQNKMTINMEMRWIDTFLRDLDQAGNRLSFSIIIAALIIG
                     SAYLLAYSTPPLVYGISLIGLIGFSAAAVLGGWLLIAILKKGML"
BASE COUNT         2989 a         2904 c         2452 g         3272 t
ORIGIN      
        1 ccagatttgc cgtgcgggac tgatggcctg ccgtgtaggg gtcccgcgct tccagggcgg
       61 aaatgagcac ctgtatggtg gtaccgacgg cctttttcag gctctccagt gttttttgca
      121 gcttttcatc ggctattttt ctctccgtta tatcgtgggc aatgcctctg aatcctttcg
      181 ggttacccga agaatctctc aaaagggata taaatccttc aatatacttt ctcgtgccgt
      241 cttttccacg gatttgccac tccagatccc tgtagggttc tccggtcata taaacctgat
      301 ggaaggcgcg atacactttt ttccgttctt ccgcgtccgt gtagatttta ttactcatgc
      361 ccgtcaattc gtctctggag tatccaagga cccggcacac agtatcatta aagaacgtaa
      421 agtttccgaa gaggtcggtt tcaaaatagc cttccttgat gtcctccaga atcgtcctgt
      481 acttctcttc actctgacgc agtttatctt ccgctatttt gcgatccgtg atatttctcg
      541 cctcacctaa aatggaaatc ggttcattat tttcattgcg tataaaatta aaggaacatt
      601 ccccccatat cgttttacct tctttagtcc ggaattccag ttccagtgtt ctatattgag
      661 aatccagaga agccagatta aaggctttcg gcatcctatt ggtaataaac tccatggctt
      721 ttctgaccga ctcgggagta agcagttgat ccaaaggcaa tatttttatt tcttcaaacg
      781 aatatcccgt gattctttcc acggacgggc tgacataagt aatctgcatg ttcatatcca
      841 tcatccagac ctgatctttc atgtgatcgg ccagaagacg gtatttttcc tcactcgcct
      901 tcagagcttc ttgagcaagt ttgcggtcgg tgatatcccg ggtttctccc aaaagagata
      961 ccggtttccc atttttatcg cggataaaat gcaggcggtt ctcgatccat attttatggc
     1021 cgtccttgtg ataggcttcc agttccatga tgtagttcgt gccggttgga ggaggcttgc
     1081 tgttcatcgc tttctccaat tctttcatga atgtttccac gactttcctg taggagtctg
     1141 ccgtaaaaag ttgcctcatt ttgatctggc tgatttcatc cagagcatat ccgtatattt
     1201 tctccacgga aggactgatg tagataaggt tgaggttcaa atccataacc cagacatgtt
     1261 ccgagatgtt gtccgccaac agccggtaga gcgcttcact ttctctcagt gcctcttccg
     1321 cctgcttacg ttcggtgata tcatgaacaa tgcctttgaa acctgttggt ttccccgaag
     1381 catccttgat caaggatacg gaggtttcaa tatatttttt atttccgtct ttcctgatga
     1441 gctgccaatc gaacgctttg gtcgattctc ccgttttata gaccctgttg aaggtctcaa
     1501 aaagtttttt tgcattttct ttgtcggtaa attgacggtt attcattccc cgcatttcat
     1561 ccggagcata tcccagaatc cggcacatcg ccttgttgaa gaatgtaaag ttgccggcaa
     1621 gatccacttc atagtatccc tcttcaatgg cttccagaag ggatccgtat tgttcttcgt
     1681 tttcacttga ctgtctgttt tgctgttcca ttttttcctg tcttccttac atgtggaaat
     1741 ggcgccgatc aatgtcgggt tcagaccttt attcattcat catgaaaacg gtatataaat
     1801 aaaggttttg tgtcaaagaa taaaattccg gatactttca gttttggata ttgaaacgta
     1861 tcatccgatt gttattcaga aagaccacga tgacgctatt tccccaatac cgtcagaacg
     1921 gtgaaacaat ataccttgca tcaacatcgg cctgttcaca caaatgatca aattttatat
     1981 accgttttga tatccatcac gttctgactg ttttcctgaa ttctctgtga ttgaacgcct
     2041 ccagcagttc cgtatcagac atttcattgt accctttcca gccgttatga agaatacgca
     2101 ccagaaattt gatgctcttt tcctgcgaca agccatccaa atcagaatct accagttttt
     2161 taatgatctc ctccctgctg atttcatttt ttgtcataaa cctgttcctc cctgataaaa
     2221 aataaaccgg acatcacaac aggatttatt aatcagtgca cgacaacttc acggaaggtt
     2281 gaatggttaa ataatttaca tctatgatac tggttatatt aagtttagca cggcttttct
     2341 gaaaaatcca gcagctgatc taaatattat cccgctcctt gttcagaata tagacgaacg
     2401 cgattttaag tgtggcttca acagcttccg gctcgatatg ctcaacgttg tcctgaagag
     2461 tgtgatagac cattccgtcc cggattaaat tcgtcggcat accgatgagc gttgtcgctt
     2521 ctacgcctgc cttggcaaat tccgccgcat ccgtggcgcc gcctccgggc gttattgcaa
     2581 agagccgcgt ccggtatccg agttgatcag ccactgcctt gcattcccgg gccatgtttt
     2641 tagacagacg aacgacgctg ttaatatctc tagtgagaaa ctggagttca ccgaggttgt
     2701 agatgctgtc gatattgaaa tgatacgtcg gcatcttcag caaggcgcct cgatgccgtt
     2761 ttacaaaagc ccgcgcgcct cgaagaccgg cctcttccgc atcggcgctt agaaacatca
     2821 gacgggtatt tttcagaagc cccgcaccgt tttgctttgc cctgttgaaa atttccgcaa
     2881 gtttaataat cgccgcgact gcaatcatgt tatcaccggc tcccggagtg cccttctcac
     2941 ttataaagaa atacatcggg gcaacaaaaa tcagaccggc aaaaacaccg tagcgtaaat
     3001 aggggacgaa ggcgggatcg gcgccgctat aggaccggtg atacacccaa atccaggaga
     3061 gaaccaatgc ggcaaaaatg attatgacgg ccgtcatgat ccggacggca tataatttct
     3121 gatgatgtgc gagaaacctg aattcatgcg cgctgtcgtg atgcccgctc aggatgatct
     3181 gttgtttgac ctcgccttcc ggttctatgg ttccgataac gttgtaccct ttcattttcc
     3241 tgtaaaaagg atccagcatt tcccagtaaa atattgattc gcccaacatg atgaaagcac
     3301 ccaaaaggta gccggccgca ccggcaacga cataatcaaa atacagcaaa aacgaggaca
     3361 ggatgtagat cacgacgaca accttgaaaa accccagaaa cgctttgggg tgaatatcaa
     3421 attcctccat gcttacggaa tcgcagtgct tattcatttc attcataatg agaacagccg
     3481 cttttttaca ggattcactc cctgcgagac gcgcaccgga ttcctctata atgttcccgg
     3541 taaaacaaag cgtttcctga acatcagccg gtgtaatttt tatgtcttcc acagtatgcc
     3601 tcctggtttc tgaaaattaa aaccgatctg cccataaatg ctgaagcttt cccgaactat
     3661 tttctattat ggtgctttac gtaacataaa agacggaaag aaagaagtga tgaattcggc
     3721 tggcgacaat cccggaagtg cttactgaaa aaaagaccag cctcgatctt tgaaaatcaa
     3781 ggctggtcgt atatcccacg tcttagcggt tgactcgtta acgtctgtct cttctttcgg
     3841 acgtcctgaa atcacctcgg cttttgacgt tatgctgcat cgcgggcttc gtcatggccg
     3901 gtcgcgtgtt gcttctgtat tgcggcgcag gggctctctt tgttattttt ttaactccat
     3961 agctttgttt atcccagtgc cggtttttct cccagacatg ccaattgcgt tggatgtcct
     4021 gatatgacct ccgctggggt tcccagcgat atccattcca cgttttatgc ctgtaattct
     4081 gtcgccaatc ggcaggaaca tatctctgga aagacggctg atagggatga taagcccaat
     4141 ctctgtcgta gtatcttgat cggtaccagc ggttattcca aggacgccac caccagcccg
     4201 cgtgaaaaaa tatgtcttct ccaatgtcgg ctacggcata gacatcagtg tccggtatga
     4261 ccaccagatg tggcggcgac ggaaagacaa ccggtagagg caaaggaata ttcacatgga
     4321 cccttacatc ggccattgcc gaaaccggca tcaatacaat cagcgtcagc aacagagcct
     4381 tgtaaacctt atttttcatt ttatgttctc ctttttgttt attctaataa catgttgcat
     4441 catccatatt tccggaatga aaaaaacatc cggctcatta cggtaagtag aggcaattcc
     4501 catgaccatt ccattgctca ccccttttcc cgccgcgcgg accgaatccg ctttttttgt
     4561 tccatccgca tttggccagt ttttccttct gtttgggtgt caaaattttg ttcacctcaa
     4621 agcggtaagc ggttttatta tcctccagtt gatcccgcag cacccttact tctttttgtt
     4681 tggccataat cttgttttta tccggatcga cctgcagcca taacttacgc aatgcagcag
     4741 atttgtcgcg tatcttttcc cgcagggggt gcaccgcctt aaaatgctgc gactgcaagg
     4801 catcaagttt tgttttctga tcttcagtca cattcaattc ttccaaaacg actccccgac
     4861 aatatccgga ctttttcccc ggacccggcc cccacgcaaa tgcctgagac gtcaaaagca
     4921 accccagaaa tacggcgatc attgtaaatg ttattttctt catcacttgt ctcctttttt
     4981 taaagttacc ctcttgaaca gcaaagcccg tgccataaaa tgtaagcgat tgtaataatt
     5041 aaactaatta gttttatcgc tattttccta acatcatttt acagatcatg ggtgggcata
     5101 ttgtacccat ttcggataca aaaccggacc catcgtaacc acaaatgact atcaggtaag
     5161 aatctcttaa cttgtcggca aatgttcagt ttctcatttt ttaaaacgtc gaagttgtaa
     5221 aacaaagcaa attggtagga tatgcctttt ctgatgatat gggtttgttt tcaaagacaa
     5281 agggtagtaa aaaaagctct cattgaaaaa aaggtatcct cgccactata catacaggat
     5341 gggatgcata gcttagacac cttgggggga ttcggcgagg atgatttctt ataatacaaa
     5401 tataataatt tatcaaccta tataaaataa aattttccgc caccctgtta ttatcacata
     5461 acatcacaga aaccggagtg aaaaaaaaca ggccctgtgc ataacgattc cggatatcgg
     5521 ccattttcat caggggcagg cgccccgctc cctggcccat gacaataatt cgcagttttt
     5581 cagttcgcat gctttttgcg catcattaca gcactgcttt ttgtcgccct gaagaaaata
     5641 aacaatgccc cggttattgt acgcatcggc ataatcaggc ttcagactga tggcatggtt
     5701 aaagttattg atggctcgct gatattgccc aagattggcg tacgccacgc ccatcttgtc
     5761 aaatattttc gtcaaagaat tttcatcgtt tgtaaaaagc aacgccagat tataatcctg
     5821 tattgcacgt tgatagtcgc ccgtctgatt gtacgccatg cctctgctga tgtaagcgtt
     5881 tgcataattc ggctgttgtt caatggcctt gtcgagatat gccatggctt ctttcacttc
     5941 aataaatttt ttcccgtccc ataaaccatc cgctctgtta aaccagtcag cagctgtaga
     6001 cgtttcaccc atggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaac
     6121 cacgaacaga agagagtttc tcctgccaat tgaaatagag aatcagccgg gacagtatca
     6181 gcggcaaaac aatgagcagg agcataattt gcagaagttt atagggatct tcaaacacgg
     6241 tcccgataaa aatccataaa atgaccggca tgatcagcaa ggcggcaatg taagatgcaa
     6301 cggtgccgga cagtgtgtaa tcaacattac cttccagtat tgccgtgaac ggaatcactg
     6361 ccaccgcggg cggcacggac gcaataagaa caaagccaat ccacagattt ttatcttcaa
     6421 tgagcaaagc cgacaaacac aacaggacac cggccagaat gatataactc ataaacattc
     6481 cggcaaggga tggtttcagt atggcacgcg gattcagaaa gtaattattg ggaacattga
     6541 ccgttgccag cgtcatcacc aatgccaggg cgggaatcat caaaactttg gtccattccg
     6601 ccggctgcgg caaaaaaaga cccaagatca tcgcaagaat aaaaataaaa ttacggttat
     6661 tcagaacttt catcgccatt agtatatgat gtcctctccg gattcgacaa gatacataag
     6721 gctgtcgcct gttctgcgca aaagggaaag tttcatagcg ttgccgggta aaaagaccct
     6781 tccatgcaat agtccgcctg acgtctgtgt cctataaata atttcggaaa tatgttccgg
     6841 aaaactgaaa aagcgggaaa gattcgggtt ggttcaccgc tacactgtca cacgagacaa
     6901 tgactccttc tcgctgtttg ttttttcagc cgtttaaatt atgttcccag actcacttca
     6961 tccgcgctat tgccggtccc cttccggcga cacattcttc aatcaattct ttaaaaaaac
     7021 aatgccgggg ttctgccggc agaataggag cgccacacat tttctttatg acgaggcaga
     7081 accttcttcg gtactttgcg ttttctcatc ttcctgtttc atggcttccc gctttgctcg
     7141 ttccttatta agctcctgca gcaggtcatt ggtataatgc agcaatcgtt ccgcttcgct
     7201 ttccagtttt cgcattccct tttccagggc atcgccttcc agctcaatgt cgattaagtc
     7261 atcatcaatt tcttcattca ttatgactac tccctttcac aatccggtgg atcatcattc
     7321 gatctcatca gccggcccac gtggcccgaa aacgatattt tttccataaa aatatttccg
     7381 attctgaaga atttttttac gcacatttca tcctttcaaa acgttaatgc cctcatggaa
     7441 acactgtatc ccataccagt tttctacgat gttttcaccg aatcttttct agggatcgaa
     7501 cgcctaacca tctctttttg ctcataggcc agacaaataa tccgccaacc gccgtcacga
     7561 cagcataatg tcagaacatc aatacctttg cgaatttctc tttctgccgt caggatgtaa
     7621 ttggctttga tgacggcaat attgccgtgt ctgttaattt ttacatgatg ccatttttca
     7681 tcaaataatg cattttcagc ggcatattcg cgttgctccg ttataccttc ggttatgctg
     7741 acaaatgaaa cagggtttcc cttttgcacc cgcaccatga tcgcccgttt atcaaacaaa
     7801 tccatccatt catccaaacg tttttgcgaa tacagccggg gataaagttc aacaatgctt
     7861 tcaagactgt agggctgccg atcatttcgt ttttgcataa ttccctgtct ttcgcgatga
     7921 cctgttgctc tgattttttt tgagtaaaaa tgaaataccc ttattatttt tccagaacat
     7981 gaacttctcc tgcttcgttg acctgccatt ttttattgac cttctgaaga cggaatcgga
     8041 tcaccagcac ttcgtcattt aggactccat cggcctgaat cgcttttaca tacgctgtgg
     8101 caacggcttg cgctgccgga agctcaagcg tttcaatttc cagatcctcg agttcgaccg
     8161 aaagattttt gtaatagcct cgggccgtca tcaggtatac cggaacatca tgtcggacaa
     8221 cagccctgga aattccgtag ctcggaatat caacctgaca gttttcggcc atcccttcct
     8281 gaatgcgtct gatgcgatgc gccaccgcca gttcgttttc ccctgttgac cattccagtt
     8341 ctttcgccag tgcctgcaaa tgttttttaa ccgctcgggc ctcccaatca acaaaaaata
     8401 agaaatacac cgcaaaagcc gaaacaagaa tcaatcccat tgccagatat tttcttttta
     8461 ccataacggc actcccacga tacggctgac cggcgcctgt ccgaaggtat gcgcttcagc
     8521 aggcattgcg cgattgtctc ccacaacata cacgtgatcc ggtttgacga taataggttc
     8581 aaggttccag ttactttttt tcttgacgta gggttcatta atttttttgt cgtccacgaa
     8641 aagaatccct ttgctgaatt caacgcgctg ccccgaaacc gccacaacgc gcttcaaaag
     8701 catgatcctt tgtcccgcca tccgcaccgt cacaacatcc ggcggtgccg gtgaggcaaa
     8761 catataacgc agggcaaaac agaaattgaa cgatccggta acataggtcg gcgccatgct
     8821 ctcgccctga atacgaaaag gaatgaaaac atatttaaac agaataaaag ctaccgcagc
     8881 cacggcggca aggcgcagca catagcgagc ggttatgtca gggaagaaga atcttctgat
     8941 ggccgttttg ttcatgaatc cttgacgctt tttgggaaat ctttcctctt gtttatttct
     9001 tgacaatata aatcacgacg agaatggcca gcacgataaa aattgcgcca tagcctgttt
     9061 ccgtaacgcc gctccatcta aacacatctc cgataatttc cgctcccatg gcatcgaacg
     9121 cccggataat ataatggccg atttcatttg cgactttatc catgttttat cctcccgatt
     9181 tcctgcaaat ccgctcaacc ggcactcgta tcataatcat aaaacgtcgc cccgcgtatt
     9241 ttcatcgtta tacccgtcat cggccattca tgatgccctc ggcggcgatc atgccggaca
     9301 tggccgctcc cacaataccg cggcttttgc cggtaccgtc ccctgcgaca aagatgcctt
     9361 tgacgtttgt ttccagataa tggtcggtcg ggaaatgcgt atcgaaaaat tttatttccg
     9421 gcgcatacat taacgttgac ggatgcaaaa tgcccgggat gattttgtcc agcgatttga
     9481 gcgattccca cagattgtcc atgatgcgtg cgggcatggc cagggtgatg tcgccggggg
     9541 ttgcgctgca ggtcgccctg cagacttcaa aatgccgcgc cgtgctgttg aacgtggaca
     9601 gcgaacttct cctgccttcg cggaaatccc cgatgcgctg gacgatcggc ttgccgcctc
     9661 cgagcaggaa aaattgtttg gcaatactca atccgaattc cgtagtatcc gaataaggtt
     9721 tggtcagggc aacggtgttg atcagggcaa aattggtatt ctttgttttc ttccctgaca
     9781 gggcatcgcc attgaccagt ttaaaatcac cgtagctttc cacccggacc cgcccgccgg
     9841 gattggtaca aaacgtcctg accttatcgc cgtggcgtcc cgtcgtaaaa ataaactttg
     9901 gatcgtaaac gataccggtc acaaagtcgt agaattttct gttgagctcc aggcggatcc
     9961 ccacatcgat cggaccgaaa ttattccgga tgtccagtgc tctggcgata tcgcggaacc
    10021 agtaggcgcc gctgcggccc ggagcggcca gaatgaccgg cgcgcaacag ctctttcgtt
    10081 ttttatcgat gcggcagagc agtcggaagg agccctcccg ttttttttcg acggactcga
    10141 cctctgtgct cagcagaaaa tcggtttttt tcagctgtcc ccgcagataa tccaccagag
    10201 ccctgccgtt atccgttccc cagtgccact gttcgggcgc aataaactgt gcaccgtgtt
    10261 cggcggccat atccgccaga gcccgaagat tatttctgtt atcggtaaag gtaacctgac
    10321 ggcagcgcgg aaatcccgta aatatacgct tgatttccag catcataccc tccgccgtta
    10381 cacggtcgat ttgcagttca tcgatgtcca gaccgacgcg gtaatcgaag tttagtttgc
    10441 cgtcgttgag aagtccgccg gcgggatatg gattgcgatc gatgacggct atgtcgtcca
    10501 ttccgagttt ttccagccga agcgcagcaa acagccctgc cggaccggaa ccgataatca
    10561 agattttata gtttttcatt tgcatcacat taaagacgaa aagcatttac accggttata
    10621 agaatgacgg ccttttgacc ccaatccagg catgaccaat cttcacggac acttttttct
    10681 tattttacat aattagcgcc ggttatcaag tcatgttatt ttatcaatga taccctgtat
    10741 tttctccatc tacaaatcgg catgtccgct gtttgccaca tgacacagca agagagatct
    10801 attgtttttt tcatgcggga tgcctataat ataattacaa tcttcagtca aaggaggtga
    10861 taagatcagc gcaaaaaaaa gcagttggtg tgagtcactt atctgttttg gtgatcatac
    10921 gtgaaaggag ggatggtcat gtttgcacca aaaagaattc tcgtaccgac agatttctcc
    10981 gattactccg atgaagcctt gaaacaggcg ttggaactcg caaaacaata ccactcgaaa
    11041 gtttatctgc ttcacgtcgc aaatccggta acacaatgca cagccgatta ttgtctggat
    11101 gtggacacgt tcaagaaagc cgaagaaagc acaatgatta aagcaaggga tatgatgcaa
    11161 caagagctgc agaaattccc tgaatcccag gagatcgaaa ttatcagtga tatcagagag
    11221 ggagaaccgg tggcagaaat tctgaaagaa caggaagaaa aaggtgtgga cttaatcgtg
    11281 atgccgtcgc acggcagatc aggactcttg aagaggctca tgggtcagat tgccgagaag
    11341 gtcatggagg caaccaagac ccctgttctg cttgtgcacc actgatgggt tcgaaaaatc
    11401 aagaagcaat gacacaaggc ggtgttgatt caacaccgcc ttgatcttgt tcagagcatc
    11461 ccttttttca ggatggcgat cagaagccag ccgcccagaa cagcggctgc ggaaaacccg
    11521 atcaacccga tgagggatat tccatacacc agcggcgggg tgctgtaggc gagaagatac
    11581 gcggaaccga tgatcagggc ggcaatgata atggaaaagg acagacggtt gccggcctga
    11641 tcaagatcgc gcaaaaaggt atcgatccat cgcatttcca tattgatggt cattttgttc
    11701 tgacgaatca gtctggt
//
LOCUS       DAOSME010000063        11508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6467_length_11508_cov_20.332751, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000063 DAOSME010000000
VERSION     DAOSME010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_11508_cov_20.332751"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLG98_09025"
     CDS             complement(<1..441)
                     /locus_tag="PLG98_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE33441.1"
                     /translation="MKKKSKTSQKSILINKKTSSKTKSSSKSSPALKKEASNSSKSKP
                     AKTESKKSFSLDRDIHEVIAREWQTAFDAVTDAICLLDKNQRILRSNRAMQDLFAVSG
                     KKIVGKYCWEIIHGTAKPVPGCPIVKSSRSRKPEKMELKTKGRTF"
     gene            complement(619..3129)
                     /locus_tag="PLG98_09030"
     CDS             complement(619..3129)
                     /locus_tag="PLG98_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOE33442.1"
                     /translation="MRRPLILLLIAMIAGILAGSYWIIPFYSLCAAAVVTLILILVSL
                     RKKWLPAGFVFIFLAVFLLGFFNIQKAFHFPESRPHILSYANEGKITVEGFVSESPVA
                     YADKNVLIVQPLRILKDKTYRPVFGAVRLSIPSGLTFYLGDFIRFQSSLRKIENFNNP
                     GALNYKRLMNLQGIYISGYIHDASDIILLRKQPSFGVHRMIESFRLYLKRIIGNHAPM
                     PEKSIIEAMTLGNQNEIPSVIRDNFSKTGTSHILSISGLHVGMVAASFFVLIFLILKS
                     SEYLMLKFNIVKIAAGFSFVIVLLYAVIAGMGVTVMRATFMAFVFLLALIFGRQKELY
                     NTLAIAGLMILAVAPEALFDISFQLSFVSVLAIIYITPRLNGLIQHKNLPLPSWLQSP
                     FRYLYMTIMVCIAATLGTLPLTVFYFDRVSLVTIAANLIAVPLLGTLTLALAMFFILS
                     AFFSEVVAGFFVQLAAFATRISVNIINHLADFTWSSVSLSKPSLMEIALFYLLIFFAV
                     QWIDEIRKKKNRLHFSSSRFAFINYALIFIVVMIGANAAYLTLRERISNDLTVTVIDV
                     GQGNSALVQFPKGTNMMIDGGGFSKSSFDVGKGIVAPFLHKQRIRRIETVVLSHPHPD
                     HLLGLIYILNHFNVSQIWKTALPVNPDIYPEWESAVKTNRIKTLLLGVTAGEKNVNGV
                     RIKILWPPDHVDPGGKALSHNDENDLSMVMKITYGHVSFLFPGDISSKVENILVRSGA
                     DLRSDVLVVPHHGSNHSSSSDFIRAVGCRFAVVSAGRSNVFRHPHPSVLQRYRQAGVY
                     IFRTDCDGAVTFKTDGHHLRTETFFQKTADIPEKQQIR"
     gene            3228..4913
                     /locus_tag="PLG98_09035"
     CDS             3228..4913
                     /locus_tag="PLG98_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33443.1"
                     /translation="MTTSNRDESANGKNKRRILSLIISRIAITTLLLAITVFIDTRRQ
                     LFGIPYAAINVFYFIVVVIYLFSSLYIVLYKLKINYLYNLYLQIAIDIVTVTFLIFMF
                     GNTQIDYSLFYTLIIIYSALFLGRKGGLIVASISSIFYGIFLNLEFYKLMPSQSLIKL
                     DYDMNAADALTNLVVRITSFYVLAFLASFIIEQEKKTSSLLEEKESEFNQLDLLFRSI
                     VESVYSGVMTVNLNNIIKTFNTAAEEITGFPRTRVQGLNIQNIFPEFLPYLNKETIDE
                     QAKARIEIIIQGRKKEKINLGVSVSPLIGKSEKQIGNILIFQNITQIKQMEKALEKNK
                     NMALIGEMAAGWAHEVRNPLAAITGSIELLKQGLNPDGTNKRLMEIVLRGKDQLENFV
                     RNFLMLARTVPAAREMVDINRIIDEIQEALQIDKDWNDTIKIKKILSGDVSVYANKEQ
                     IRQVLNNIINNAVEAMEEGGLLSIGTRVVQTSGKKKYAEIVVSDTGCGIKEEDLNKIF
                     EPFFTKKTRGTGLGLAIVNHLVEGYKGKIEIESTEGKGTRCRLLLPVEKDENL"
     gene            4955..6313
                     /locus_tag="PLG98_09040"
     CDS             4955..6313
                     /locus_tag="PLG98_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE33444.1"
                     /translation="MAKILIVDDDQGMREMLEIMLQKEGYDVTSIGDPVKAVDLCRKK
                     NFDMVITDLKMPKIDGIEFLKSVKDQRPETIVILITAYASGETAINAMKEGAYDYVEK
                     GRSLDELKKVVRAALMNKGLISDDPAEQKCTGATESNHAFCGMIGTSREMLKIFNTIK
                     KVSDTPANILILGESGTGKELVARAIHENSSRSKMPFVAINSGGIPENLLESELFGYM
                     KGSFTGAYADRQGYFEMARGGTIFLDEIGELSPSLQVKLLRVVQEKTFRRIGGAEDIK
                     VDVRIISATNKNLQERVKKGDFREDLYYRLNVIPILIPPLRERREDIPLLIDTFIEKY
                     SKMFGKEIRTISSYAVELLLNYDFPGNIRELQNIIERGVAMESSNIILPESLTFGAPK
                     FDVDISDQGIDLNAELDKIEKTIIEKALQKTKGSKKKAAELLNVTFDSLRHRIEKLGV
                     ES"
     gene            6603..7805
                     /locus_tag="PLG98_09045"
     CDS             6603..7805
                     /locus_tag="PLG98_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE33445.1"
                     /translation="MKSRILIAILSAVFLIAGTGSLQAKTIKVGAAINLTGPASTWGQ
                     FHEKGQRDYFRYVNEVKGGVYGNKIEMITVDTAYKVPEAQAAVNKFVLQDKVDMIATW
                     GAGEGLAAKPIIQRYKVPAINYSTSWEILEKPVDYMYLPFGSYKMDCHAVFEYIKSIH
                     KGKDAPKVGLLTYNNAYGRSIHAPSKAYAKQLGINLVAVEEFPPKTVDLNTELLRLQK
                     AGAQYIFMQMLPSAIITAFKSADRINYNPVFLGTWTSTDPDFFKMGKGLIRDRLVIQF
                     PGGLPSDKSKGMAVMQELWKRYKTVSKFDASYWEGVVVGMIMERAFMRAYEKSKNINP
                     ATINAAMESFRNEDFGGLVPAITYTKDNHGASFTARMVKVKEDGTYIPLTNFYVPGRD
                     KIKLLGSK"
     gene            7875..8702
                     /locus_tag="PLG98_09050"
     CDS             7875..8702
                     /locus_tag="PLG98_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE33446.1"
                     /translation="MKAFRSRPSIMINRREENKKAELAIQNLRLSFGGVVAVKDVDLQ
                     VHTGELMAIIGPNGAGKTSLLNCITGFYRAQQGKIVFNGKEITRLHTHELVGVGIGRT
                     FQNIELFPGMTVLANMTLARHIHCRYSFIKSFLFSKSVRDEEIRHRQVLEEIIDFLEM
                     QSIRKQAVGSLPYGMQKRVELGRALALEPKLLVLDEPFAGMNMEEKEDMVRFLLELNQ
                     QWGLTMILVEHDMSIVMSISQRIMVLNFGEKLGEGNAEEISANPEVIKAYLGESEAM"
     gene            8724..10649
                     /locus_tag="PLG98_09055"
     CDS             8724..10649
                     /locus_tag="PLG98_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE33447.1"
                     /translation="MSKGTSHITKRNLTLPQVLMENAAVYGDAKTAIREKAYGIWQKY
                     TWADYGRYVRMTAAGLAALNLRRGENLCMVVHNHPEWLFSELAAHVLGATTLNLFTSS
                     IADELAFSIKRINSPVVVVQDQEQVDKLLEIKEKLPQTQKVVYIDPTGMASYQDDPWL
                     ISYAGLLKLGEQYLEEHPGFIDSEINRGRPDDIAVMIQTSGTTGVPKLAMLSHRNLLT
                     MARQWIEETRIKPEENWLSMSPPAWIVDQMWGMGVALDGAMCMNFPETAETVLEDFRE
                     IGPSMIITSSRFWEDMASRIRVKISDAGWLKKTLFYLGEKIGKSVVEKQSHRQPVGWP
                     LQVLHSVMSVIVYRPLLDRLGCSHFRSAFTGGHPISPDVIHFFRAIGLNLKQCYGLTE
                     SCGIFQIQPDHEVKLETVGKPMVLTEVKIAGDQEVLVKSEANFSGYHNDYESTQNAFE
                     NGWLKTGDAGYIDHDGHLLIIGRKEDIIRNKSGEAFSPDFIETRLKFSPYIKEAVTFG
                     EARPYIAAMINIDMGNVGNWAEERMIPYTTYMDLSQQSAVEELILKEVIAVNEQLPEV
                     MKIKKFVLLYKLLDADDEELTRTGKVRRRFVYGLYLKIIEAMYNNLKNVQVTGKVKYR
                     DGRIGEIETTINVINVN"
     gene            10664..>11508
                     /locus_tag="PLG98_09060"
     CDS             10664..>11508
                     /locus_tag="PLG98_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE33448.1"
                     /translation="MEFFLQLLVNGVCIGLLYGISAMGFVMIFKSSSVLNFAHGELLA
                     LGAFFFLSLVTWAQLPVYVAFILTMIGCFILGFLIERFFLRPLIGEKLIFVVMLTVGL
                     AAMFKGFILLVWGGNLHTYPEFLPPSMAMQFGSIYVAPAYSATLMISVVFLVLFGLFF
                     KKSSQGIYMRSVADNQKAALSLGVHVRRVFALSWAIAALVACMSGIVLGIINGVNVHD
                     LSSIGLKVFPVVILGGLDSIGGAIIGGIIIGLLETFTGGYLSPSLRDVVPYIVLVFIL
                     MVKPYG"
BASE COUNT         3138 a         2497 c         2964 g         2909 t
ORIGIN      
        1 aaaggttcgt cctttggtct taagctccat cttttccggt ttccgggacc tcgacgattt
       61 gacgatcggg catcccggaa ccggttttgc cgtcccgtga ataatttccc agcagtattt
      121 cccgacgatt ttttttccgg atacggcaaa caggtcctgc atggcccgat tggaacgaag
      181 gatacgctgg tttttgtcaa gcagacagat ggcatcggtg accgcatcaa aggccgtttg
      241 ccattcacgg gcgatgactt catgaatatc ccggtcgaga gaaaaagatt ttttcgattc
      301 tgttttggcc ggtttgcttt ttgaggagtt tgatgcctcc ttttttaaag ccggcgaaga
      361 tttggatgag gattttgttt tgctgctcgt ttttttgttt ataaggatgc ttttttgtga
      421 ggtctttgat ttttttttca tggcgtaata tcccttttgt cgattgtgta atgtgaaggt
      481 ttttcagcaa agaacgccgc cggcacaatg tgatcaaaga acgggtttcc tcaaggtcct
      541 aaaaaagaaa gcactaacaa aaaacgggat aagtacatca tttacttgaa aataattata
      601 ggctgataat ttataaagtc aacggatttg ttgtttttcg ggaatgtcgg cagtcttttg
      661 aaaaaaggtt tccgtgcgca gatgatgtcc gtcagtttta aaggttaccg caccgtcgca
      721 atcggtgcga aaaatataca ccccggcttg ccggtatctc tgcaaaacgg aaggatgagg
      781 atgcctgaag acgttcgatc gtcccgcgct gacaacggca aaacggcagc cgacggcccg
      841 gatgaaatcg ctgctgctgg aatgattgga tccgtgatgc ggcacaacca ggacatcgct
      901 tcttaaatcg gcgccggagc ggacaagaat attttccacc ttcgaagata tgtcgccggg
      961 gaacaaaaaa ctgacatggc cgtaagttat tttcatcacc atcgataaat cgttttcgtc
     1021 gttatgggaa agcgccttcc cgcctggatc gacatgatcg gggggccaga gaattttaat
     1081 ccggacgccg tttacgtttt tctcgccggc ggttactcca agcaaaaggg ttttgatccg
     1141 gtttgtttta acggcgctct cccattccgg gtagatgtcc ggatttacgg gtaatgccgt
     1201 tttccagatt tgtgagacgt tgaagtggtt cagaatataa atcagtccca gcaggtgatc
     1261 gggatgcgga tggctcagaa cgaccgtttc aatgcgccgt attctttgct tgtgcagaaa
     1321 aggtgcgaca atgccttttc caacgtcgaa agaacttttg gaaaaaccgc cgccgtcaat
     1381 catcatgttt gtacctttcg gaaactgcac aagtgcggag ttgccctgtc cgacgtcgat
     1441 gaccgttacg gtcaggtcat tggagatcct ctcgcggagt gtcagataag ctgcgttggc
     1501 gccgatcatg acaacaatga aaatcaaggc ataattgatg aaggcaaagc ggctggatga
     1561 aaagtgtaaa cgattttttt tcttcctgat ttcgtcaatc cattgcaccg caaaaaaaat
     1621 caataagtaa aataaggcga tttccatcag cgacggttta gacagggaaa ccgacgacca
     1681 ggtaaaatcg gcaagatgat tgatgatgtt gactgaaatc cgggtggcga aggccgccag
     1741 ttggacaaag aatccggcaa cgacctcgga aaaaaaggcg ctcagaataa aaaacatggc
     1801 caaggcgagg gtcagggtgc ccagaagagg aacggcaata agattagccg caatcgtcac
     1861 cagcgacaca cggtcgaaat aaaatacggt cagaggaagc gtgcccagtg tggcggcaat
     1921 acagaccata atggtcatgt agagataccg gaagggactt tgcagccatg acggaagcgg
     1981 gaggttttta tgctgaatca atccgttcag tcgcggcgtg atatagatga tggccagaac
     2041 ggaaacgaag gaaagctgaa atgaaatatc aaaaagcgct tcgggcgcaa ccgccagaat
     2101 catcagaccg gcaatcgcca aggtattgta aagctctttt tgccttccga agatcagcgc
     2161 gagcagaaaa acgaatgcca tgaacgtcgc ccgcattacc gtaaccccca tgcccgcgat
     2221 aacggcatac agcaggacga tcacgaaaga aaatcctgcc gcaattttga cgatgttgaa
     2281 tttgagcatc aggtattccg atgattttaa aatcagaaaa atcagcacaa aaaaagaagc
     2341 ggccaccatg ccgacgtgca ggccggaaat ggaaagaata tgcgacgttc cggtcttgct
     2401 gaaattgtcc ctgataacgg acgggatttc attttgattg ccgagcgtca tcgcttcgat
     2461 aatgcttttt tccggcatcg gggcatggtt gccgatgatg cgtttgagat aaagccggaa
     2521 ggattcgatc attctatgaa cgccgaacga aggttgtttt ctcaacagaa tgatatcaga
     2581 ggcgtcgtgg atatatccgg aaatataaat gccctgcagg ttcatcaggc gcttgtagtt
     2641 caacgctccc ggattgttga aattctctat tttccttaaa gacgactgaa aacggatgaa
     2701 gtctcctaag taaaaggtca ggccggaggg aatggacaga cggacagccc cgaagaccgg
     2761 acgatatgtt ttatctttta gaatgcgcag aggttgcacg atgagaacat ttttatcggc
     2821 ataggcgacg ggactttcag aaacaaagcc ttcaacggtg atttttccct cgttcgcata
     2881 agaaagaatg tggggccggc tttcgggaaa gtgaaacgcc ttttggatgt tgaaaaagcc
     2941 caaaagaaag acggccagaa agataaagac aaaaccggcc ggcagccatt ttttccggag
     3001 actgaccagg ataaggatta gggtgacaac ggcagcggcg cacaagctgt aaaacggaat
     3061 gatccaataa ctgccggcaa ggatgcccgc aatcatggcg atcaagagaa gaatcaacgg
     3121 tcggcgcata atcgtttttg actttcaacc gcatgcatga aatatattag cgaaaagtgc
     3181 taaaatatta taatatattt cgcaaatgtt ttttttgagg aatctgcatg acgacatcga
     3241 atagggacga gtccgcaaac gggaagaata aacgcaggat tttatcgtta atcatctcca
     3301 ggatagcgat taccacgctt ctgcttgcaa ttactgtttt tatcgatacc cgtaggcagt
     3361 tgttcggcat cccctatgcc gcgattaacg ttttttactt catcgttgtt gttatttatt
     3421 tattttcctc tctgtacatc gttttgtaca aactcaagat caattacctc tataatttat
     3481 acctgcagat agcgatcgat attgttaccg ttacgtttct gattttcatg ttcggcaaca
     3541 cccagattga ttactccctt ttttatacgc tgatcattat ttattcagcg ctctttctgg
     3601 gacggaaagg aggcctgatt gtcgcctcca tttccagtat attttacggg atatttctga
     3661 acctcgagtt ttataaattg atgccgagcc agtctctcat taaactggat tacgacatga
     3721 acgcggccga tgcgctgacc aaccttgtcg tgcgcatcac ttctttttat gttctggctt
     3781 ttctggccag cttcattatc gaacaggaaa aaaagacctc cagcctgctg gaggaaaagg
     3841 aatcggaatt caatcaactg gatttgcttt tccgaagcat tgtggaatcg gtttacagcg
     3901 gggtcatgac ggtgaatctg aataacatca tcaaaacatt caatacggcg gctgaagaaa
     3961 tcaccgggtt tccccgcacc agggttcagg ggctcaatat tcaaaacatc tttcccgaat
     4021 ttctgcccta tctgaacaag gaaaccattg acgaacaagc caaggcgaga attgaaatca
     4081 ttattcaagg cagaaaaaaa gaaaaaatca atctgggtgt gtccgtatcc ccgctgatcg
     4141 gaaaatccga aaaacagata ggcaatatcc tgattttcca aaatattacg caaatcaagc
     4201 aaatggaaaa ggctctggag aaaaacaaaa atatggcttt aatcggggag atggccgcag
     4261 gctgggcgca tgaggtcaga aatccgctgg cggcgattac cggttcaatt gagctgttga
     4321 agcagggact gaatccggac ggaaccaata agcgcctcat ggaaattgtc ctgcggggca
     4381 aggatcaact ggaaaatttt gtgcggaatt ttttgatgct ggccaggacc gttccggcag
     4441 cccgggaaat ggtggatatc aaccggatca tcgatgagat tcaggaagct ctccagattg
     4501 acaaggactg gaacgacaca ataaaaatca aaaaaatatt atccggagac gtcagcgttt
     4561 atgccaacaa agagcaaata agacaagttt tgaataacat catcaacaat gcagtcgagg
     4621 ccatggaaga gggcggtttg ctgtccattg gaacccgagt ggtccagacg tcgggtaaga
     4681 aaaaatatgc ggaaattgtc gtatccgata ccggatgcgg tatcaaagaa gaggatttaa
     4741 ataaaatatt cgagcctttc tttaccaaaa aaacaagggg gaccggttta gggctggcca
     4801 tcgtcaatca tctggttgaa ggctataagg gaaaaattga aatagaaagc acggaaggca
     4861 aaggaacccg gtgccgttta ctgttgccgg ttgaaaagga tgaaaatttg tgatttagaa
     4921 tttgacaatg cgttgttgag acaatgaggt cgatatggca aagattttga ttgttgatga
     4981 tgatcagggc atgagggaaa tgcttgaaat catgctgcag aaagaagggt atgatgtcac
     5041 cagcatcggc gatcccgtga aagcagtcga tttatgccgg aaaaaaaatt tcgatatggt
     5101 gatcaccgat ttaaagatgc ctaagatcga cggaattgaa tttctcaaaa gcgtcaagga
     5161 tcagaggccg gagaccatcg ttattctcat caccgcctat gcttccggag agacggcgat
     5221 caacgcgatg aaagaaggcg cgtacgatta tgtggaaaag ggacgcagtc tcgacgaact
     5281 caaaaaggtt gtgcgtgccg cgttgatgaa taaaggcctg atcagcgacg atcccgcgga
     5341 gcaaaaatgc accggcgcca cggaatcaaa ccatgccttt tgcggcatga tcggcacaag
     5401 ccgggaaatg ctcaaaatat tcaacacgat taaaaaggtt tccgacacgc cggcgaacat
     5461 tctgattctc ggggaaagcg ggacgggcaa ggaactggtt gcccgcgcca ttcatgaaaa
     5521 ttcttcccgg tctaaaatgc cgtttgtcgc gatcaacagc ggcggcatac cggaaaatct
     5581 tctggaaagc gagctcttcg gatacatgaa agggtctttt acgggagctt atgccgaccg
     5641 gcaggggtat tttgaaatgg cccgcggcgg aacgattttt ctcgatgaaa tcggagaact
     5701 atcgccgtct ctgcaggtca aattgctgcg ggtggttcag gaaaagacat tccgtcgcat
     5761 cggcggtgcg gaagacatta aagtggatgt gcgcattatt tccgcgacca ataaaaatct
     5821 tcaggaaagg gtgaagaaag gggactttcg cgaagacctt tattatcgcc tgaacgttat
     5881 accgatcttg atccctccgc tgagggaaag acgggaggat atcccgctgc tgattgatac
     5941 cttcatcgag aaatactcaa aaatgttcgg gaaagaaatc agaaccattt cttcgtacgc
     6001 ggtggagctt ttattgaatt acgactttcc gggcaatatc cgcgaactgc agaatatcat
     6061 cgaacgcggc gttgccatgg aaagctccaa cattattctg ccggaaagcc tgactttcgg
     6121 agccccaaaa ttcgatgtgg atatttcaga ccagggcatt gatttgaacg cggaactgga
     6181 caaaatcgag aaaacaatca ttgaaaaggc attgcagaaa acaaagggat cgaagaagaa
     6241 agccgccgag cttttaaatg ttacgtttga ttccctgcgg catcgtatcg aaaaactggg
     6301 tgtggaatcg taaatggtgg tattaagaaa aattataccg aaaccttctt tttgcttgac
     6361 ttttcaatag cgtttgctct atatgactcc acattgggag tttaatgaat atgttaagga
     6421 tttatttttt taaaaaggca gtcttttttt tcttttttag ctttatacgt aaagtctgcc
     6481 tgatagtatc atgaccatcc gtaagtggta atgaaccgtg ggcagataaa aagcccacgg
     6541 tttttttttg ccgtcatcag gacggccgtt ttaaaaaaag aaagaagggg aggggaataa
     6601 aaatgaaaag cagaatttta atagcgattt tatcagcagt ttttttgatc gccggtaccg
     6661 gaagcttgca ggccaaaacc atcaaggtcg gagcggcgat caatttgacc ggtccggctt
     6721 caacctgggg gcagtttcac gaaaagggac agcgcgatta tttccgttac gtcaacgagg
     6781 tgaaaggcgg cgtttatgga aacaaaattg aaatgatcac ggtggatacc gcttacaaag
     6841 taccggaagc gcaggccgcg gtaaataaat tcgttctgca ggacaaagtg gatatgattg
     6901 ccacatgggg agccggcgaa ggcctggcgg ctaagccgat catccagaga tacaaggttc
     6961 cagccatcaa ttattccacc agttgggaga ttttggagaa acccgttgat tatatgtacc
     7021 tgccgttcgg cagttacaag atggattgcc atgcggtttt tgagtatatt aagtccattc
     7081 ataaagggaa agatgctccc aaagtcggtc ttctaaccta caacaacgcc tacggccgtt
     7141 ccatccatgc gccgagcaaa gcatacgcga aacaactggg catcaatctg gtcgccgttg
     7201 aggaatttcc tccgaaaacg gttgatctga atacggaact tctccgtctg cagaaggcgg
     7261 gcgcgcagta tatttttatg caaatgcttc cgtccgcaat tatcacggcg ttcaaaagcg
     7321 ccgaccgcat caattacaat ccggtattcc tgggcacctg gacatcgacg gatcccgact
     7381 ttttcaaaat gggcaaggga ctgattcgcg accggctggt cattcagttt cccggaggat
     7441 tgccttccga caagtccaag ggaatggccg tcatgcaaga actgtggaaa cggtataaaa
     7501 ccgtcagcaa atttgatgcg tcctactggg aaggcgttgt tgtcggcatg attatggaac
     7561 gtgcctttat gcgtgcttat gagaaaagta aaaacattaa tccggcaaca atcaatgcgg
     7621 cgatggaatc attcagaaac gaagatttcg gcggtttggt tccggccatt acctatacaa
     7681 aagacaatca tggcgcttcg tttactgcgc ggatggttaa agtgaaggaa gacggaacgt
     7741 atattccgct gacgaatttc tacgttcccg gcagagataa aatcaagctg ctgggcagta
     7801 aataaatcat gatggcacca ggcgtgatca gaaccttgat tctgatcacg ccgttttcta
     7861 aagaagaggt ttgaatgaaa gcgtttcgca gccggccgtc gattatgatc aaccggcggg
     7921 aagagaacaa gaaagccgaa ctggctattc agaacctgcg cctgagtttc ggcggtgttg
     7981 ttgccgtcaa agatgttgat ctgcaggttc ataccggtga attgatggcg attatcggtc
     8041 ccaacggagc ggggaaaacg agcctgctca actgcatcac cggtttttac cgcgctcagc
     8101 agggtaaaat cgtcttcaac gggaaagaga tcacccgtct gcatacgcac gaacttgtcg
     8161 gcgtgggcat cggcagaact tttcagaata tcgagctttt tccgggcatg accgttctgg
     8221 ccaatatgac actggcgagg catattcatt gccggtacag tttcataaaa tcatttctct
     8281 tttccaaatc agtccgggat gaagaaatcc gtcaccggca ggtccttgaa gagatcatcg
     8341 attttctgga aatgcaatcc atccggaagc aggcggtcgg ttctctgcct tacggcatgc
     8401 agaagagagt tgaactggga cgcgcgctgg ctttggagcc gaagctgctg gtcctggatg
     8461 agccgtttgc cggcatgaac atggaagaga aggaggatat ggtgcgcttc cttctggagc
     8521 tgaatcagca atggggcttg acgatgatcc tggttgaaca tgatatgtcc atcgtcatga
     8581 gtatttccca gcggatcatg gttttgaatt tcggagagaa gctgggtgaa ggaaacgctg
     8641 aagaaatcag cgcaaatcct gaagttataa aggcttatct cggcgagtcc gaagcaatgt
     8701 aaatatttta aggcgtttta attttgagca agggaacatc acatatcacg aaacgcaatc
     8761 tgacattgcc gcaggtgtta atggaaaatg ccgccgtgta cggtgatgca aaaacggcta
     8821 tccgggaaaa ggcatacggt atctggcaga aatacacctg ggcggattac ggtcgctatg
     8881 ttcggatgac ggcggccgga ttggcagcac tgaatctcag gcgcggtgaa aatctgtgca
     8941 tggtcgtcca taatcatccg gaatggctct tttcggaact ggccgcgcat gtcctggggg
     9001 caacaacgct gaatttgttt acatcatcca ttgccgacga actggcgttt tcaatcaaaa
     9061 ggatcaactc acccgttgtg gtggttcagg atcaggaaca ggtggataaa cttctggaga
     9121 tcaaagaaaa acttcctcag acccaaaagg ttgtttatat cgatcccacg ggcatggctt
     9181 cttatcagga cgatccctgg ctgatcagtt acgccggact cttaaaactg ggcgaacaat
     9241 atcttgaaga acatcccggc tttattgact ccgaaatcaa tcggggacgc cccgatgata
     9301 ttgccgttat gatccagaca tcggggacca caggcgttcc caagctggcc atgctgtccc
     9361 atcgcaacct cctgaccatg gccaggcaat ggattgaaga aaccaggatc aaaccggagg
     9421 aaaactggtt gtccatgtcg ccgccggcct ggatcgtcga tcagatgtgg ggcatgggtg
     9481 tggcgcttga cggcgccatg tgcatgaact tcccggaaac ggcggaaacc gttctggaag
     9541 atttcagaga aatcggacct tcgatgatca ttacatcgtc gcggttctgg gaagacatgg
     9601 cttcgcgcat ccgcgtaaaa atttccgatg ccggttggct gaagaagaca ttattttatc
     9661 tgggcgaaaa aatcggcaaa tcggtggtgg aaaaacagtc gcaccgacaa ccggtcggat
     9721 ggccgttgca ggttttgcac agcgtcatgt ccgtgattgt ttacaggccg cttctggatc
     9781 gtctgggatg ttcgcatttc cgcagcgcct ttaccggcgg acatccgatc agtcccgatg
     9841 tcattcattt cttccgggcc atcggtctga atttaaaaca atgctacggt ttgacggaat
     9901 cgtgcggcat tttccagatc cagccggatc atgaagtcaa actggaaacc gtcggcaaac
     9961 ccatggtgct gaccgaggtg aaaatcgccg gggatcagga agtgctggtc aaatccgaag
    10021 cgaatttttc aggctatcac aatgattatg aatcaacgca aaacgcgttt gaaaacggct
    10081 ggctcaaaac cggcgatgcc ggatatatcg accatgacgg tcatctgttg attatcggcc
    10141 gcaaggaaga tattatccgc aataaaagcg gcgaggcttt ctcgcctgat tttatcgaga
    10201 cgcgtcttaa attcagtccg tatatcaagg aagctgtgac attcggcgaa gcccgtcctt
    10261 acattgcggc gatgatcaac attgatatgg gcaatgtcgg caactgggcg gaggaaagaa
    10321 tgattcctta tacgacgtac atggatctct cccagcagtc ggcggtggaa gaattgattt
    10381 tgaaggaagt cattgcggtg aatgagcagc ttcccgaggt catgaagatt aaaaagtttg
    10441 ttctgcttta caaactgctg gatgcggatg atgaggaact gacccgcacc ggcaaagttc
    10501 gccgccgctt cgtttacggt ttgtatctga agatcataga ggcgatgtat aacaatttga
    10561 aaaatgttca ggttacggga aaagtgaagt accgcgatgg aaggatcggc gagatagaaa
    10621 caacgattaa tgttattaat gtaaattaag ggacttatta gagatggaat tttttctgca
    10681 attattggtc aacggcgttt gcatcggact gctctacggc atttccgcga tgggctttgt
    10741 catgattttt aaatcgtcaa gtgtgttgaa ttttgcccac ggtgaacttc tggcgctggg
    10801 agcgtttttc tttctgtcgc tggtcacgtg ggcgcagttg ccggtttacg tagcttttat
    10861 tctgaccatg atcggctgtt ttatccttgg ttttctcatc gaaagattct tcctgcggcc
    10921 gttaatcggc gagaaactca ttttcgtggt tatgctgact gtgggactgg cggcaatgtt
    10981 caaaggtttt attctgctcg tatggggcgg aaatttgcac acctatccgg aattcctgcc
    11041 gccatccatg gcgatgcagt tcggttccat ttatgtcgcg ccggcctatt cggcgacgct
    11101 gatgatcagc gttgtttttc tggtattatt cggactgttt tttaaaaaat catcccaggg
    11161 aatttatatg cgctccgtgg ccgataacca gaaagccgcg ctttctcttg gcgttcatgt
    11221 tcgccgggtg tttgcgcttt cctgggcgat tgccgcgctg gtggcctgta tgagcggcat
    11281 tgttctgggc atcatcaacg gcgtcaatgt gcatgatctg agctccatcg gactcaaggt
    11341 tttcccggtt gttattctgg gcggcctgga cagcatcggc ggcgccatta tcggcggaat
    11401 catcatcggc cttctggaaa cctttaccgg cggctacctg tcgccgtctt tgcgcgatgt
    11461 tgttccttat atcgtgctgg tgtttatttt aatggtcaag ccttacgg
//
LOCUS       DAOSME010000064        11215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6664_length_11215_cov_23.052599, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000064 DAOSME010000000
VERSION     DAOSME010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6664_length_11215_cov_23.052599"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..684
                     /locus_tag="PLG98_09065"
     CDS             268..684
                     /locus_tag="PLG98_09065"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOE33449.1"
                     /translation="MIIYDLKCENGHAFEGWFKDRENFVLQHSKKLVSCPICNSSGVE
                     IVPSSITIMGKESRTENKSRSKELSGMGALKMLHQYIDKNFEDVGDKFAEVALKIHYG
                     DEEKRNIKGTSTPREEETLKEEGVQYFKIPVAKMDS"
     gene            773..3094
                     /gene="cysS"
                     /locus_tag="PLG98_09070"
     CDS             773..3094
                     /gene="cysS"
                     /locus_tag="PLG98_09070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOE33450.1"
                     /translation="MSTFYKNVMEGIGNTPLVEITRLNPNKKVRILAKMESANPGGSI
                     KDRTALFMIESAESRGNLTHDKIILEATSGNTGIGLAMIAAVKGYKLCLIMPESASEE
                     RKKILRAMGAELYFTPAGHGTDGAIEVAYKMLRENPDKYFGTDQFNNEDNVAAHYFGT
                     GQEIWDQTDGKVTMVVASLGTSGTAMGISKRLKELNPQIKMVGVEPYLQHKIQGLKNM
                     RESYRPGIFNKNMLDEKINIQDEDAFEMSRRLAREEGLFVGMSSGAALFVALQQARQM
                     DEGLIVVIFPDGGERYLSTELFAVKKAISTFYLFNAFARKKTLFNPIKTDEVAMRTCG
                     PTIHDTPHLGNYRRLVVSDLINRYLIYKGYKVKHVLDIVDFGDKSIRGSEKESVALAE
                     YSKKYLQTFIEDTKFLNIRQDNICVKSSENVEAMLKMVEKLTDKEYAYEKLHSVYYNI
                     SKLADYGALSNIDLGKTRKVKSVDVDDYEKDSPADFALLKRSSLKELKRGIYYKTVWG
                     NVRPSWHLECAAISQKYLGDTYDIHVSGADETFPHCENVLAIQKAFSGHQGPNYWMNV
                     ELVMVEGRKMSRSLNNALSLSGLQKDGYSGSDIRLFLLSIHYRKPVNYSAGALQTAKN
                     TVRKIHTFIRRLHAIDDGVGGNGFAETDQLIYDLKQQFAAALDDDLNIAGALAALFSF
                     IGKINEPLTKGLINKNNAVKILKALENINIVLGVMDFEVGTIHRNIEELIDKRNAARK
                     ARDWKEADSCRARLAELGVDVLDTPRGVIWRFK"
     gene            3099..3926
                     /gene="amrB"
                     /locus_tag="PLG98_09075"
     CDS             3099..3926
                     /gene="amrB"
                     /locus_tag="PLG98_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOE33451.1"
                     /translation="MNPIKKSVIAGSWYPGNPAVLRRDIEKYFDAVPEMTIDGNIVGL
                     IAPHAGYLYSGHVAAHAYKLVRGRTYDAVMVVGPSHRVAFRGISIFSKGGYETPLGVV
                     PVEEALADKLKKKSPIVTDIPQAHLQEHAVEIQLPFLQVALGQFSFLPLVMGSQDAAT
                     CRELAEAIADVSADKNLLIVGSSDLSHFYNYNAAKKMDAVALGHLKHGDSQGLLQSLE
                     NGDVEACGGGPMAVVMSVARMLKADRPCLLKYANSGDVTGDKESVVGYAAAVFYRSP"
     gene            4003..4551
                     /gene="amrA"
                     /locus_tag="PLG98_09080"
     CDS             4003..4551
                     /gene="amrA"
                     /locus_tag="PLG98_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOE33452.1"
                     /translation="MELTDQEKNTLLDIAKNAIAAKINHRETPELNIDSNNLRLKRGA
                     FVTLKKQGRLRGCIGYIKAYKPLWETVQEMAVAAAFHDPRFPSLRAEEIQQLTFEISV
                     LSPLKRIADVNDIEVGRHGLYIIRGHHSGLLLPQVATEYKWDRETFLEETCHKAGLPP
                     DAWKDKETEIYIFSADYFGDIS"
     gene            4605..5219
                     /locus_tag="PLG98_09085"
     CDS             4605..5219
                     /locus_tag="PLG98_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOE33453.1"
                     /translation="MIRRHKGLACFIFVLLLTAGMVLASPYFLLYATDYKKADAVILL
                     LGPDFKARHKAARELVASGMADYLIISAYNKTYRNHDQDGTRQLVLDKHAPDADSENN
                     APFPGFYEDTHIEIIKAKKVMTAYGLKSAIFVSSPYHMRRIRFMAAKEFNFNDASFYF
                     VPTRYEKAPANFWELSSSDWRKVRREYSKIIWFFIYSAWLDYFL"
     gene            complement(5299..5538)
                     /locus_tag="PLG98_09090"
     CDS             complement(5299..5538)
                     /locus_tag="PLG98_09090"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOE33454.1"
                     /translation="MLATHKIHQKDFNNLKQVFMLSAWGFTMVVVSFLFLYIGHLVDG
                     LLGTTPNFMLGFFILGLFLCMMRLYQEACIRRKDV"
     gene            complement(5581..5856)
                     /locus_tag="PLG98_09095"
     CDS             complement(5581..5856)
                     /locus_tag="PLG98_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33455.1"
                     /translation="MKKTLGTMIVAAAVILFTATFSFAEYAAAGAANFPFFQLGCLIV
                     GGLIMVSLKRKYDKMYVGEVVGIFALYTILMALFTNPVIETVKTIVS"
     gene            complement(6155..8458)
                     /locus_tag="PLG98_09100"
     CDS             complement(6155..8458)
                     /locus_tag="PLG98_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE33456.1"
                     /translation="MVGSLDKISASAPGNSKIRKELLDITNRIHAAQNIKQILVDLKS
                     EILKLFNANSLTIYVVDKLRNEIYSMFLAGTQLKEIRLPINNKSIAGYVANTGAVINI
                     ENAYNAKELKSIEAELTFDDSWDKKTGFKTRQVLAAPIYHNNQLMGAIQIINKKVGEG
                     KFSEDEIGLALEIAEVLGVAFYNQQRFVRHRKTRFDYLLSHDLIKEDELESAWKESRE
                     KKISMENFLMQKHKISKADIGHAFEEFYNCRFIQYNDKLPIPGHLIKNLKKNFLRREL
                     WVPLEMVGDKIHVIIDDPNNIIKKDTIESLLKTKKIKYDVALEEDIIKYVNLFYNTPD
                     EEHSFTEILGRMEGEDGGPSSDDDEDLGEGVSETDSVIMQLVNKIVNDAYNRRASDIH
                     VEPNVGKKNVEIRFRVDGDCSLYQTVPFSYRAAIISRIKIMSNLDITIKRLPQDGKIK
                     FRRNTGDEIELRVATIPTQGGVEDVVMRILAKGETMPLQDMGLLPRNYNELINIISKP
                     YGMILVVGPTGSGKTTTLHAALHHINRPDRKIWTAEDPVEITQYGLRQVQVQPKIGFD
                     FAAAMRSFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETIVRLLD
                     MGIDPLNFADALLGILAQRLVRTLCKSCKEPYHASKDEYDEIAKSYGAEEFAKLNIPY
                     NDDFKLYRAKGCPNCDGTGYKGRMGIHELLVGSEDIKRMIQKHETVEVMREKAISEGM
                     TTLLQDGLLKTLKGFTDFKQVRRVCIK"
     gene            complement(8560..10632)
                     /gene="glyS"
                     /locus_tag="PLG98_09105"
     CDS             complement(8560..10632)
                     /gene="glyS"
                     /locus_tag="PLG98_09105"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOE33457.1"
                     /translation="MSNELLFEIGTEEIPAGFLSKAVVDMEEIIRKSFTDKRIAFDGI
                     KCLATPRRLVLYIADLSAKQEDQTVEKLGPAKKAAFDENGQPTKAAIGFARGQGVDVS
                     QLETITTEKGEYLGARKTIAGQPTKDLLPDILKDFMLAIPFRKSMRWADYTLRFARPI
                     HWILALYSGEVIPLKIEDIESGNTSCGHRFMSPAPFAVAGFEDYLNKARENFVIADPA
                     ERRKLILEEAEKSAAEVGGKLFYTDDLLETVSFIVEYPVIVRGGYEEDYLKIPKDVLT
                     TTMISHQKYFPVVNEAGKLLPYFIAVSNTKPRDISVVAKGNERVLRARLADASFFFEE
                     DKKVPLEDRVESLKKVVFHTLLGTSHKKVARFRKLAIKIASKVKPSAKKNVDRAALLA
                     KADLESLMVGEFSELQGIMGREYALIAGEKPEIANAIYEHYLPIVAGGDLPQSDEGAI
                     VGLADKMDTIAGFFGVGMPPTGTADPYALRRQALGIINIILSREYPLSLNFLIDESLA
                     LLKDVLKKPAEGIKKDILEFFKGRLQNQLIAQGYAYDTVDAVLSANIDELVEVIEKVK
                     ALEEFRKHPDFEPVSIAFKRVDNILKDFKGGQFDVNLLVHDAEINLFSYFDNIKPRVE
                     KGIAEKDFAAALNKLAALRPHVDNFFDNVMVMDKDEKVRFNRQSLLAEISALFHKIAD
                     FSKIVTAG"
     gene            complement(10731..>11215)
                     /locus_tag="PLG98_09110"
     CDS             complement(10731..>11215)
                     /locus_tag="PLG98_09110"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOE33458.1"
                     /translation="ISQFTYFQQVGGFDCSPVCAELTYGTERIAMYIQGVNNVYDLQW
                     TDKIKYGDVHHRGEVEFSTYNFEVADIPMLRKLFDTYEEEALRIAEKNLALPAYDYCL
                     KCSHTFNLLNARGAISVAERTSYIGRVRNLARISAELYMKQREELGYPLLSRSAYSDL
                     "
BASE COUNT         2879 a         2547 c         2837 g         2952 t
ORIGIN      
        1 gatggcgcga tttgaacgcg cgactcctgc gtcccgaacg cagtgcccta ccaaactggg
       61 ccacatcccg actcacggcg gacgatataa aatttatttg caattgtcca actttttttg
      121 ttcatttttc cgttttcttt atcatttaca tcaaaaatat gattttaacg gaaaggaata
      181 aagctcgaaa gaattgatat gtaattgtaa tttataaaca ggcatgttat aaataatgca
      241 aatgggtcat aaaacaggtt atttattatg attatatacg atttgaagtg tgaaaacgga
      301 cacgcgtttg aagggtggtt caaagatcgg gaaaactttg ttctgcaaca ttcaaaaaaa
      361 ctggtttcct gcccgatctg taacagctcc ggtgtggaga ttgttccgtc ctcaattacg
      421 atcatgggaa aagaatcccg gacggaaaat aaatcccgga gcaaggaact gtctgggatg
      481 ggcgctctga aaatgctgca tcagtatatt gataaaaatt tcgaagatgt gggcgataaa
      541 tttgcggaag tggcgctgaa gattcattac ggagatgaag aaaaaagaaa catcaagggt
      601 accagcacgc cccgggagga ggaaacctta aaagaagagg gggtgcaata ttttaaaatt
      661 cccgtggcca aaatggatag ttgattcacg tccggaaatg aataaagatt cccaaagatg
      721 ctgattattt tttaacccct gcgggttttt ttattttaag gtatttttac gtatgagtac
      781 attctataaa aatgtcatgg aagggatcgg caatacgccg ctggtggaaa ttaccaggct
      841 caatcccaat aaaaaggtca gaattctggc taaaatggaa agcgccaatc ccggcggatc
      901 catcaaagac cgaaccgccc tgtttatgat cgaaagcgcg gaatccagag gtaatctgac
      961 gcacgacaaa attattctgg aagcaacaag cggtaacacc ggtatcggcc tggccatgat
     1021 tgcagccgtc aaaggctata aactgtgcct gatcatgccg gaatcggcca gtgaagaaag
     1081 aaaaaagatt ctgcgggcga tgggggccga attatacttt acgccggccg gccacggcac
     1141 cgacggcgcc atagaagtgg cctataaaat gctgcgcgag aatccggata aatatttcgg
     1201 gacggatcag tttaacaatg aagataatgt tgccgctcat tatttcggca ccggacagga
     1261 aatatgggat cagacggacg gaaaagtgac catggtggtg gcgagtctcg gtaccagcgg
     1321 gacggccatg ggcatttcca aaaggctcaa agaactgaat ccgcagatca aaatggtcgg
     1381 tgtcgaaccc tacctccagc ataagataca ggggttgaaa aatatgcggg aatcctatcg
     1441 tcccgggata ttcaacaaaa acatgctgga tgagaagatc aacattcagg atgaggatgc
     1501 ctttgaaatg tcccggaggc tggcccggga ggaaggtctc tttgtgggca tgagttcggg
     1561 cgcagccctg ttcgtggctt tgcagcaggc ccggcagatg gacgagggac tgatcgttgt
     1621 gatcttcccc gacggcggag aaagatattt aagcacggaa ctttttgccg taaaaaaagc
     1681 aatatccacc ttttatcttt tcaatgcgtt tgcgcgcaaa aaaacgcttt ttaatccgat
     1741 taaaacggat gaagtggcca tgcggacctg cgggccgacc atacacgata cgcctcattt
     1801 gggtaattac cggcgccttg tggtatccga tctgatcaac cgttatttga tctacaaggg
     1861 atacaaggtg aagcatgttc tggatattgt cgatttcgga gacaaatcca tcagaggatc
     1921 ggaaaaagaa tccgtggcgc ttgcggagta ctcgaaaaaa tatttgcaga cgttcattga
     1981 ggatacgaaa tttctcaaca ttcgtcagga caatatctgt gtcaagtcct ctgaaaatgt
     2041 ggaggcgatg ttgaagatgg tcgaaaaact aacggataaa gagtacgcgt atgaaaagct
     2101 gcattcggtg tattacaata tatccaagct tgcggattac ggcgccctgt ccaatatcga
     2161 tctcggcaaa accagaaagg tcaaatccgt tgatgttgat gattacgaaa aagacagccc
     2221 ggcggatttt gccctgctga agcggtcgag tctgaaagag ctcaaaagag ggatttatta
     2281 taaaacggta tggggaaatg tcagaccgag ctggcatctg gagtgcgcgg caatttctca
     2341 gaagtatctg ggagacacgt atgacattca tgtttccggc gcggatgaga cgtttcctca
     2401 ctgcgaaaac gttttagcca ttcagaaggc tttttccgga catcaaggcc ccaattactg
     2461 gatgaacgtc gaactggtca tggtggaggg gcgcaaaatg tcccgttcgc tgaacaatgc
     2521 cttatcgctg tccgggttgc agaaggacgg ttacagcggc agcgatatcc gtctttttct
     2581 gctgagcatc cattaccgga aaccggtcaa ttattcggcg ggggctttac agacggccaa
     2641 gaataccgtc agaaaaattc atacgtttat tcgacggctt catgcgatcg atgacggtgt
     2701 cggcgggaac ggttttgcag aaaccgatca gttgatatac gatctcaagc agcagtttgc
     2761 cgccgcgttg gatgacgatc tgaacatcgc gggagcgctg gccgctttgt tttctttcat
     2821 cggtaagatt aacgagcctc tgacgaaagg cttgatcaat aagaataatg cggtaaagat
     2881 tttaaaagcg ctggaaaata tcaatattgt tctgggggtc atggatttcg aggtcgggac
     2941 gattcaccgg aatatagaag aattgatcga caaacgcaac gcggccagaa aagcccggga
     3001 ctggaaagaa gcggattcct gcagggcccg tctggcggag ctgggcgtgg atgttttgga
     3061 caccccccgg ggtgtcatct ggcggtttaa atagagagat gaatcctatc aagaaatcag
     3121 tgatagccgg ttcctggtat ccgggaaatc ctgccgtttt gcgcagggac atcgaaaagt
     3181 attttgatgc cgttcccgaa atgacgattg acggcaatat cgtcgggttg atcgccccgc
     3241 atgccggcta cttgtattcg ggacacgtcg cggcccatgc ttacaaactg gtccgcggca
     3301 ggacatacga tgcggtgatg gtcgtaggac ccagccaccg ggttgctttc cgcggcattt
     3361 cgattttcag caagggcggc tacgaaacgc ctttgggtgt cgttcccgtt gaagaagctc
     3421 tggcggacaa gctgaagaaa aaaagtccga tcgtcacgga cattccgcaa gcccatctgc
     3481 aggagcatgc cgtggaaata cagcttccct ttctgcaggt tgccctgggc caattttcat
     3541 ttttaccgct ggtcatgggc agtcaggatg ccgccacctg ccgggagctg gctgaagcga
     3601 tagctgacgt atcggcagat aaaaatctgc tgattgtggg aagctccgat ttgtcgcatt
     3661 tttataacta caatgcagca aagaaaatgg atgccgttgc cctgggccat ttgaaacacg
     3721 gcgactcaca ggggcttttg caaagtctgg aaaatggtga cgtggaagcc tgcgggggcg
     3781 gacccatggc ggttgtgatg tctgttgccc gcatgttaaa ggccgacagg ccgtgtcttt
     3841 tgaaatacgc caattccgga gacgtcacgg gggataagga atccgttgtc ggatacgccg
     3901 ccgccgtttt ttaccgttct ccttaaaagg gatcttgttt attttctttc gtaacgacgg
     3961 aaaaatgtat gcgtcattga atgatggaga aaggaaagca acatggaact gacagatcag
     4021 gaaaaaaaca cgcttctgga catcgccaaa aatgcgattg ccgctaaaat caatcatcgg
     4081 gaaactcccg agttgaacat tgactccaac aatctgcggt tgaaaagagg cgcattcgtc
     4141 accctgaaga agcaggggcg gttgcgcggt tgcatcggtt atattaaagc atacaaaccg
     4201 ctctgggaaa ccgtgcagga aatggccgtt gccgcggctt tccatgatcc gcgattcccg
     4261 tctttaagag cggaagaaat tcaacaactg acctttgaaa tatccgtctt gagtccgctc
     4321 aagcgcattg cggatgtcaa cgacattgaa gtcggcaggc acggtttgta catcatccgc
     4381 gggcatcact cgggtttgtt gctgccgcag gtggcaacgg aatataaatg ggacagagaa
     4441 acatttctgg aggagacctg ccacaaagcc ggattgccgc cggatgcctg gaaggataag
     4501 gaaacggaaa tctacatttt ttcagcagat tatttcggcg acatttcctg aaaaaccgtt
     4561 cagacaattc atgctcattt tgtaaaacga ttgaagagat gattatgatc agacgtcata
     4621 agggattggc atgtttcatt ttcgttctgc tgttgacggc cggaatggtt ctggcttctc
     4681 cgtattttct gctttacgcc acggattata aaaaagcgga tgccgtcatt cttttgctgg
     4741 gaccggattt caaggcgcgc cacaaggcgg ccagggaact ggttgcttcc ggcatggcgg
     4801 attatctgat tatttcagcc tataataaaa cctacagaaa tcatgatcag gacgggacgc
     4861 ggcagcttgt tctggataag catgcgccgg atgctgattc agaaaacaat gcgccttttc
     4921 cgggttttta tgaagacacg catatcgaaa tcatcaaagc caaaaaggtc atgaccgcct
     4981 atggccttaa atcggcaatt tttgtaagct ctccctatca tatgcgaagg atacgattca
     5041 tggcggcgaa ggaattcaat tttaacgacg cctcttttta ttttgttccg acgcgctatg
     5101 agaaagcgcc ggccaatttc tgggaattat cgtcttcgga ttggagaaag gtcaggaggg
     5161 aatacagtaa aataatctgg ttttttattt attccgcctg gttggattat ttcctttgat
     5221 tttgatcaga aaaaccctcc tgaaaacata gcgtcttgca ggagggtcat atgatatagc
     5281 agatcatgtt tttaatgatc agacatcctt ccttcgaata caggcttcct gataaagcct
     5341 catcatgcac aggaaaagtc ccagaataaa aaaccccagc ataaaattcg gtgtggttcc
     5401 cagcagaccg tcgaccagat gtccgatata caggaacagg aaagaaacaa ctaccatggt
     5461 gaaaccccac gcggagagca taaagacctg tttgagatta ttgaaatctt tttggtgtat
     5521 tttatgagtt gccagcatga ttcattcctc ctttcccgtg agatgttgtt ttagcaatgc
     5581 ttagctgacg atggttttga ccgtttcgat gaccgggttg gtgaacagcg ccatcagaat
     5641 ggtgtacagg gcgaagatgc cgaccacttc accgacatac atcttgtcat atttgcgttt
     5701 caaagaaacc atgatcagtc cgccgacgat caggcatccc agctggaaga aggggaaatt
     5761 ggctgctccg gctgccgcat attccgcaaa gctgaaggtt gctgtgaaaa gaataaccgc
     5821 tgccgccact atcattgttc ctaatgtttt tttcatgatc tgttgcctcc tttaggaaat
     5881 ttttttcttt gtcttaatct aatgaaaaga ccgtgccaga atgccaaaaa atcttattaa
     5941 attttgcaaa gttatgattt tattaaatat tattattaga taagttattt attgcttatg
     6001 ggaaaacacg attatttcgt ctttttatga ataaaatatt aaagatgttt aacgaaatgt
     6061 cgatgccggg gatccgttga cggacaaata agaaaaaagg agatcaccga atgaagagaa
     6121 atttttgatg ggagaaaatc cgcggacaag cggattattt gatacaaacc cggcgcacct
     6181 gcttgaagtc tgtaaatcct ttaagcgtct tcagcagccc gtcctgcaaa agtgtggtca
     6241 tgccttcgct gatggctttt tcccgcatga cctccacggt ttcatgtttt tgtatcattc
     6301 ttttaatatc ttccgaaccg acaaggagtt catggatacc cattcgtcct ttatatccgg
     6361 ttccgtcgca attgggacat ccttttgctc tgtataattt aaagtcgtca ttgtacggaa
     6421 tgttgagttt ggcgaattct tccgcaccgt aacttttagc gatttcgtcg tattcgtctt
     6481 tggaagcatg atacggttcc ttacaggact tgcaaagcgt gcgcacgaga cgttgcgcga
     6541 ggatgcccag caacgcgtcg gcgaaattca gcggatcgat gcccatatcc agcagacgga
     6601 cgatggtttc cggagcgctg ttggtgtgca gggtgctgaa aaccaggtgg ccggtgaggg
     6661 aggcttccac gccggttttg gctgtttcaa aatcgcgcat ttcaccgacc atgatgacat
     6721 ccggatcggc gcgcagaaag gaacgcatgg ccgccgcaaa atcgaaaccg atcttcggct
     6781 gcacctggac ctggcgcaaa ccgtattggg tgatttccac gggatcttcc gctgtccaga
     6841 tttttctgtc cgggcgatta atatgatgca gggccgcgtg cagcgtcgtg gttttaccgg
     6901 aaccggtcgg cccgacaacc agaatcatgc cgtacggctt ggaaataatg ttaatgagtt
     6961 cgttgtaatt gcggggcaaa aggcccatgt cctgcagggg catcgtttcg cccttggcca
     7021 gaattctcat gacgacatct tccacaccgc cctgggtggg aatcgtcgcg acacgtaatt
     7081 caatttcatc tcccgtgttg cgccggaatt taattttgcc gtcctgcgga agtctcttaa
     7141 tcgttatatc aagattggac atgattttta ttctggaaat gatggccgcg cggtagctga
     7201 aaggcacggt ttgataaaga ctgcagtcgc cgtcgacccg gaaccggatt tcaacattct
     7261 tcttgcccac attgggttcg acatggatgt cggatgcgcg gcggttatag gcgtcgttga
     7321 cgattttgtt gacgagctgc atgatcacac tgtctgtttc agagacgccc tcgcccaggt
     7381 cttcatcatc atccgatgac gggccgccgt cttctccctc catacggccg aggatttcgg
     7441 tgaaggaatg ttcttcatcc ggggtgttat agaagagatt gacgtatttg ataatgtctt
     7501 cttccagggc gacatcgtat ttaatcttct ttgttttcag gagactttcg atcgtatctt
     7561 ttttgatgat gttgttggga tcatcaatga tgacgtgaat cttatctccg accatttcca
     7621 gaggcaccca gagctcgcgg cgtagaaaat tctttttcaa atttttgatc aaatgtccgg
     7681 ggatgggcag cttatcattg tattgaatga agcggcagtt ataaaactct tcaaaagcgt
     7741 gaccgatatc ggctttggat atcttatgtt tctgcatgag aaaattttcc atggagattt
     7801 ttttctcacg cgattctttc caggcgcttt ccagttcatc ttccttgatc aaatcatggc
     7861 ttaacagata atcgaaacgc gtttttctgt gacggacgaa gcgctgctga ttataaaacg
     7921 caactcccag aacctcggcg atttccagcg caaggccgat ttcatcctcg gaaaattttc
     7981 cctcgcccac ttttttgttg ataatctgaa tggcgcccat gagctgattg ttgtgataaa
     8041 tgggcgccgc aagaacctgc ctggttttaa agccggtttt cttgtcccag ctgtcgtcga
     8101 acgtcagttc agcttctatg cttttgagct ctttggcgtt ataggcattt tcaatattga
     8161 tgaccgcgcc ggtattggcc acataaccgg caatgctctt gttgttgatc ggcagcctga
     8221 tttctttgag ttgcgtcccg gccaggaaca tggaataaat ttcgttgcgc agtttatcga
     8281 caacataaat ggtcagggaa ttggcgttga aaagttttag aatttcgctc tttaaatcaa
     8341 ccagaatctg tttgatgttt tgggcggcat gaatacggtt ggtgatatcc agcaattcct
     8401 tgcgtatttt ggaattaccg ggcgccgatg cggaaatttt gtctagagaa cctaccatga
     8461 ggcaaattat tccttagtaa tctgtatgaa aatcatagta gcaaggattt gaatttatgt
     8521 caatgcgcct catgtcccct gaaggttgcc gctttacgat cagccggccg tgacgatttt
     8581 ggagaagtcg gcgattttat ggaaaagagc cgaaatttcc gccagcagcg actgacggtt
     8641 aaaacgcact ttttcgtcct tgtccatgac catgacgttg tcgaagaaat tatccacatg
     8701 gggacgcagc gcggccagct tattgagcgc cgccgcaaag tctttttctg cgatgccttt
     8761 ttccacgcgg ggtttgatat tgtcgaaata agaaaacaga ttgatttccg catcgtgaac
     8821 aagaaggttt acgtcaaact gacctccttt aaaatccttt aaaatattgt ccacgcgctt
     8881 gaaagcgatg gagaccggct cgaaatcggg atgtttgcga aattcctcca atgccttgac
     8941 tttttcaatg acctcaacca gttcatcaat atttgcggat aaaacggcgt ccacggtatc
     9001 gtaagcataa ccctgcgcaa tcaactggtt ctggagacgt cccttgaaaa attccagaat
     9061 atcctttttg attccctccg ccggtttttt caagacatct ttcagaagcg ccaggctttc
     9121 atcgatgaga aagttgaggc tcaaggggta ctcgcgcgat aaaatgatat tgataatgcc
     9181 cagcgcctga cgccgcaggg cgtagggatc ggccgtaccg gtcggcggca tgcccacgcc
     9241 gaagaagccg gcgatggtgt ccatcttatc ggcaaggcct acaatggcgc cttcatccga
     9301 ttgcggaagg tctccgccgg cgacgatcgg caaataatgt tcataaatgg cgttggcgat
     9361 ttccggtttt tcaccggcga tgagcgcata ttcacggccc atgatgcctt gcagttccga
     9421 gaactcaccg accatcagcg attccagatc ggccttggcc aaaagcgccg cgcggtcgac
     9481 atttttcttc gcggacggtt tgaccttgga tgcaatcttt atcgccagtt tgcggaaacg
     9541 cgcaactttt ttatgcgagg ttccgagcag cgtatggaag acgacttttt tgagcgattc
     9601 cacacgatct tccagcggaa ctttcttgtc ttcttcaaaa aagaacgaag cgtcggccag
     9661 tcgcgcccgc agaacccgtt cattgccttt ggccaccacg gaaatatcgc ggggtttcgt
     9721 gttgctgacc gcaatgaaat agggcagcag ttttcccgct tcattgacga ccggaaaata
     9781 tttctgatgg gaaatcatcg tggtggtcag aacgtctttg ggaattttca gatagtcctc
     9841 ttcgtaaccg ccgcgcacga tgaccggata ctccacgata aagctgaccg tttccagcag
     9901 atcatccgtg taaaaaagtt ttccgccgac ttccgccgct gatttttcag cttcttccag
     9961 aatcagtttt cttctttccg ccggatcggc gatcacaaaa ttttctctgg ctttgttcag
    10021 ataatcttca aagccggcga cggcaaaggg ggccgggctc ataaaccgat ggccgcagga
    10081 ggtgttgccg ctttcaatat cttcgatttt caggggaatg acctcgccgc tgtacagcgc
    10141 cagaatccag tggatcgggc gcgcgaaacg caacgtgtaa tcggcccagc gcatggattt
    10201 acggaaagga atcgccagca tgaaatcttt gagaatgtcc ggcagcagat ccttggtggg
    10261 ctgtccggca atggttttac gcgcgccgag atattcgccc ttttcggttg tgatggtttc
    10321 cagttgcgaa acgtccacgc cctgtccccg ggcaaaaccg atggcggctt tcgtcggctg
    10381 accgttttca tcgaacgcgg cttttttggc cggtcccagt ttttcaacag tctggtcttc
    10441 ctgctttgca gacagatcgg cgatataaag aaccagacgg cgcggggtcg ccaggcactt
    10501 gatgccgtcg aaggcgatgc gtttatccgt aaacgatttg cggataatct cttccatatc
    10561 caccaccgct ttggataaaa atcctgcggg aatttcttcg gtgccaattt caaaaagaag
    10621 ttcgttgctc attttgtctc cagatatttt ttatctcttt aatcagaccc aaatttcaga
    10681 aacgaagtgt actgaaattt gatggtcgaa ttatccccga ggcgaataac ctaaaggtca
    10741 ctataggccg agcgggataa aaggggatag cccagttctt cacgttgttt catgtacagt
    10801 tccgcgctga tgcgcgccag atttctcacc cggccgatgt agctggtgcg ttccgccacg
    10861 ctgatggcgc cgcgggcgtt gagcaggttg aaggtatgcg aacacttcag acagtaatcg
    10921 taagcgggca gggccagatt tttctcggcg atgcgcaggg cttcctcttc atacgtatcg
    10981 aataatttgc gcagcatggg aatatccgct acttcaaaat tataggtgga aaactccact
    11041 tcgccgcggt gatggacgtc gccgtatttg attttgtccg tccactgcag atcataaaca
    11101 ttgttgacgc cctgaatata catggcgatg cgttccgtgc cgtaggtcag ttcggcgcaa
    11161 accggactgc aatcgaagcc gccgacctgc tggaaatagg tgaactggga aattt
//
LOCUS       DAOSME010000065        11215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6665_length_11215_cov_19.705645, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000065 DAOSME010000000
VERSION     DAOSME010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6665_length_11215_cov_19.705645"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLG98_09115"
     CDS             complement(<1..293)
                     /locus_tag="PLG98_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33459.1"
                     /translation="MTVVSLAVYGQVGQFDFINFDDNVYITDNHHVQSGITLASIRWA
                     LTTTYAEFWHPLTWLSLMADYELYGLHAGGYHVTNIIFHILSTLLLFWLFHRMT"
     gene            complement(417..1985)
                     /locus_tag="PLG98_09120"
     CDS             complement(417..1985)
                     /locus_tag="PLG98_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33460.1"
                     /translation="MALKIITFMALLVGGLSSYLLARHILGYTKWGSLFCGLVFGLSL
                     FVPLRVYDGNTNEVYVAFLPLCMLLIGLACRGRKTAVFILAFVFYTMLSDGKLIALMI
                     FLYLGILCLLDIIPSFNIFATKNLNKMNIKPLKVLFLALTITFFVGMLRILPVLDMIE
                     TMGGLQSNFLWFKPITYSPSGVVTLQELWHNLIGYKDILNIATIGLIPVLLALSVLFI
                     FPPKAFPWAVTIFLFTWLMLAYNAPVDLSKLLWHLPVFNIITKHKYYAFPIVFSLIIV
                     AGQFFWLLSKIRSKWLEHALAAALILLSVWFLYPRFDAIQKNTYTYDIPEEFLVRQNE
                     FYNIQGLGIPRFRAKPLNSVIYTNVIRNVGIIDSVTHYGEKAVPKYFVDAEGTLIPNP
                     GYKGEAHFADPANTAKATFRPNSIMVQVNLRKPDMLVINQNYERNWHSEHGRIFNKGG
                     LIALRLDKPGVYDITLRYFFRSFFVGLFLSIVSLIALISICWSYKTGRLIKWSHDAPI
                     HIRWMPRFILWLIN"
     gene            complement(2583..4052)
                     /locus_tag="PLG98_09125"
     CDS             complement(2583..4052)
                     /locus_tag="PLG98_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33461.1"
                     /translation="MRKILFIYPRLGYMDAQRSKPAPPLGILAAVSFLKDTYEIIILD
                     QRLFASDDSFYAKLYNVLQNQPLYAGLSVYSGGMIKFALEISRYIKEQSSVPVVWGGI
                     HPSLLPEQTLENQYIDYVIQGEGELTLPEFSDRLVQYGQNMPPVRGVWIKKQGKIAYG
                     GDRDLIDLQAAPVIPYDLVDFNNYVQYYNGHKYAYYQASRGCPRHCRYCYNHVFNKGK
                     FRAKSADKIISEIKTLAERYASFTGIFFVDDNIFAMGKDYVMNLGKGLGELGLRWAVQ
                     GSDIIALKDYTESDFKRLETCGLTRLTIGVESASKKMRTLIGKQGSPADIEQVMARLS
                     GTKILIWCSYIISLPGETLDDLRASIKLIFRLQHINPNVRNSPFYMYIPFSGTPLYEQ
                     YKDVFPGPESLEEWGQVGWEREHTNSFADYLKDTHFFQSLFLTSLLDDDKVSDFSKNK
                     LLVFLAKCYRPVARWRLKNLFFKFNIELSMFKKFFPDIF"
     gene            complement(4045..5151)
                     /locus_tag="PLG98_09130"
     CDS             complement(4045..5151)
                     /locus_tag="PLG98_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33462.1"
                     /translation="MNSLFDLSNLYGLACNIWPRFPYDIPQLQTALKPFLYLFNITNQ
                     CNLNCYYCCQKTSLTRNRPELSFEDIAGIIKKLPRYAVIVFSGGEPLCRDDFTQLLQE
                     TRRLKRKTALLTNGLLLNDDIICEMIQNRLLNIGIAIDGDKYYYEKIKGRGNYAVVMD
                     NVDRLAYHKKKAKSSFPAFDWKVTVFPENVSRLPLLYQQAADHGADSFTVSFPKKNEF
                     QFNDCLHDIAVLDCEPVSNGNYRFPENIEDIYKELFSIARTSRTRLRTYPQMKQAEDL
                     KNYFETSAIQKRYSACRMPWSGMVISSTGEAYPCLSVRIGDLKKQSLAQVLTSPENIR
                     FRNQLKKRSLFAICDGCCYARLKPVLKRERAVGA"
     gene            complement(5235..6686)
                     /locus_tag="PLG98_09135"
     CDS             complement(5235..6686)
                     /locus_tag="PLG98_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33463.1"
                     /translation="MKRPKILLFKSHSIVSKVTGVTPPLGLLYIAAALRERLGADVRV
                     IVAMLENDPMNVMVSTLRHFRPDVVGISALTAEAFFAHKAAAAVKAEAPSVPVIMGGP
                     HASCDPELVLQDSCVDAVVMGEGEDTFTELVSVTMSEKSVWQNPDILQKIDGLAFRVD
                     GKMKLSKPRAPINDLDALPFPAWDLIDYKKYWTLPKMATDGVRPYLTMFTSRGCPYNC
                     IYCHQLFGKSFRSRSPESVVEETARLMRMGAESIEILDDISNFNPERFDRMLELMLEK
                     NLHPVLSFPNALRADLIRKESADLLKCVGTGEISVAIETASDRLQKLLGKNLSLEKAK
                     RTIDWFADRRIFTRGFFMLGFPTETKDEMLATIRFAHASRLHMALFFTPNPFRNTALY
                     DLFVDTGKLPSTSTSIDYEYYGAPFNGSDVSDRRYRMLYRWAYYGFYFNPVRLWRIAQ
                     DRPTLADIPASAWTRLNNVVSFRRLKEEDTSLV"
     gene            complement(6814..8874)
                     /locus_tag="PLG98_09140"
     CDS             complement(6814..8874)
                     /locus_tag="PLG98_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33464.1"
                     /translation="MLKKMNLSSDRQRLIVYVVLIALSLFVFSQVGQFDFITFDDPVY
                     VVDNPSIQSGFTMDGVIWAFGTIHAEFWHPLTWLSLMLDYQLFGLNAGGYHITNLMLH
                     ILSTLMLFWLLHRMTGAVWRSAFVAALFAIHPLHVETVVWIAKRKDVLSAFFWMLTLC
                     LYVYYTEKPMVKRYLFVFLSFACALMSKPMAVTLPVIMIVLDYWPLKRFASKKENPVF
                     WQVKEKLRFFMLSAVFSLITIFAQYDKYIKHAETSFDSRLANAPVSLTTYLAKTLWPY
                     DLAVFYPFSYQLPAWQTAGSIALILAISVAVILAAKRLPYLLVGWLWYVLAVLPVLDI
                     FRSSARAMSDNYTYLPAVGLYVMAAWGIPDLFCQKQIRRKILWPAGIAVAAMLSVLAW
                     QQCSYWQNSFKLFNHALHVTKDNYLAYNQRGNVYSKSQQYPPAIADFNEAIRIKPDYA
                     FAFNNRGNTYLQLGRYQQAISDYSEAIRLNPDYARAYYNRGFTYGIHMGRYQRAIDDL
                     NEAIRREPDAPDIHLTRGIVYSNLGGQELACLDYQKACDLGNCKLLDMALGKGECLAA
                     DLQPHDSGYYHNSGISYFKRGQYRIALGYFTHAIHFKKDFANAYHNRGTTYSELGRYQ
                     HAVADFNESIRLNPGYPDAYKNRGIAYLLQGEKEQGCSDFKKACALGDCKLLELAESK
                     GDCR"
     gene            9068..10051
                     /locus_tag="PLG98_09145"
     CDS             9068..10051
                     /locus_tag="PLG98_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOE33465.1"
                     /translation="MSIQKHHVWEYPTLSTPKAILRFVFTWLVTLAIFVVLFSRIPFH
                     DVVSLLQQTDMRFLCAGLLCSVLAHLFFSSARFQRVVKMTGCRISFGEAVIIRMGCNP
                     IKGILPFKMGEFAILGYMKERHHLSYARGLLALLSGYMFSFTALIFFCSMGGLFYVHP
                     PLQKMLFAAACLLIVLLIAISIRQNFFRPVVLYIHKILRLPENVRKIIESYDPQTGQA
                     IMIHSLAIEGCKLLIILFSFKSLSIDLPVDALLLWGSMTIIAAYLPMTYWGLGIRESA
                     VIVLFAGYAAPGHLLAAGLLMTFVDGLLPVLLGLFFIRPFLNNWLGSMMQK"
     gene            10141..>11215
                     /locus_tag="PLG98_09150"
     CDS             10141..>11215
                     /locus_tag="PLG98_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33466.1"
                     /translation="MRLACVFNPFQYKLHEENLRVVQKYFGLFPPLSLAWVCAIAEKA
                     GHDALLIDARTLRLTREDVLDRLKQWKPDILGFMMTTYMFRETLEWVTFLKKHLKIPV
                     IIGGYNLRVYPNESLSVEGPDFGCFNSAFYTLPRLLEELEGRRRFADVPGLIYKENGR
                     TTMTPYGRDPVFDDYPNPARHLLPNDLYAEFTTERKNFTVMVTSKGCPNGCLFCEAGH
                     TSYNPRSPQTVLGEIKECRTRYGIREIDFFDYDFCIDRKRTEAICDGIIQNGMDITWA
                     CRTRVDCVDEALLRKMKAAGCSRIYFGIESGVQEILDRLNKGISLQQIRDTIETARSL
                     GIRALGFFLVGAPGETRETFQQTV"
BASE COUNT         2999 a         2558 c         2706 g         2952 t
ORIGIN      
        1 gtcatccggt gaaagagcca gaacaacaac aaggtgctga ggatatgaaa gatgatgttg
       61 gtgacatggt atcctccggc atgaagaccg taaagctcat aatcggccat cagggacagc
      121 caggtcaaag gatgccagaa ttccgcgtag gtagtcgtca atgcccagcg gatactcgcc
      181 agagtgatgc cggactggac atgatggttg tcggtaatat aaacattatc atcaaaattg
      241 atgaagtcaa attgtcccac ctgaccataa acagccagtg agacaaccgt caaaaaaata
      301 tatataaaaa ccttttgtct ctgaagaccg gtatccatcg tatttaatat atcggattat
      361 ttttgccgta cttgtactac atgttcaatc agaattcagt agttttttag taatgcttaa
      421 tttatgagcc aaaggatgaa tcgaggcatc catcttatat ggatgggggc atcatgtgac
      481 cacttgatta atcttccggt tttgtagctc caacagatag aaatcaatgc tattaggctg
      541 acaatactta agaacaaacc gacgaagaaa ctgcgaaaga agtaacgaag ggtaatgtcg
      601 taaacgccgg gcttgtccag acgcaatgca atcaaaccac ccttattgaa aatccttccg
      661 tgctccgaat gccaattcct ttcatagttt tgattgatca caagcatgtc cggttttcgc
      721 aggttcacct gaaccatgat ggaatttggc cgaaaagtgg ctttggctgt gtttgcgggg
      781 tcggcaaagt gggcttctcc tttgtatccg ggattgggga taagtgtgcc ttccgcatca
      841 acgaagtatt ttgggacggc tttttcgcca taatgtgtga cgctgtcaat gatgccgaca
      901 ttacgaatga cgtttgtata tataacggaa ttcagcggtt ttgcccgaaa gcgtggaatt
      961 cccaggccct gtatattata aaactcattt tgcctgacga gaaattcttc cgggatatcg
     1021 taagtatatg tgtttttctg aatggcatcg aaccgcggat acaaaaacca tacgctcaaa
     1081 aggattaatg cggcagccaa tgcatgttcc agccactttg aacgtatttt tgacaataac
     1141 cagaagaatt gtccggcgac aataatcaaa gagaagacta ttggaaaagc gtaatactta
     1201 tgtttagtga ttatattaaa cacggggagg tgccaaagca gctttgacag gtccacggga
     1261 gcattgtagg ccagcatcaa ccatgtaaat aaaaaaatgg taacggccca gggaaatgcc
     1321 tttgggggaa atataaacaa aacgcttaaa gcaagaagca cggggattaa tccgatcgtt
     1381 gcgatgttaa gaatatcttt atagccgatt aaattatgcc ataactcctg caacgtaaca
     1441 accccgctgg ggctgtaagt gatgggcttg aaccaaagaa agttgctttg taaccctccc
     1501 attgtttcta tcatatccaa tacgggtaaa atacgcagca taccgacaaa aaaggttatc
     1561 gtcagcgcca ggaaaagcac ttttaaaggt ttaatgttca tcttatttaa attttttgtc
     1621 gcaaaaatgt taaatgaggg aatgatatcc agcaaacata gaattccgag ataaagaaaa
     1681 atcatcaaag cgataagctt gccgtccgaa agcatggtat aaaaaacaaa agccaggatg
     1741 aagacggctg tctttcttcc gcggcatgcc agtccgataa gcagcatgca taacgggaga
     1801 aaagcgacgt aaacctcgtt agtattgccg tcgtataccc ggagggggac aaacaagctt
     1861 aaaccaaaaa ccagtccgca gaaaagcgat ccccacttgg tgtagccgag aatatgtctt
     1921 gccagtaaat aactgctcaa ccccccgacg agcaacgcca taaaagtaat gatctttaga
     1981 gccataacag atccaaaaac aatggtgatc aaagtcaacg ggtttagtgc agggtcttcc
     2041 gggttgccga gagaaggata accgcctcct atccagagcg agcgagtcgg aaaagtatga
     2101 tgttcgagga cagcttgtct gaccgattcg tgcattccgg cataataaaa gaaatcatca
     2161 ttaaagttaa ctttactccc cgatgaaagc ggtgagatcg acggcagtgc gatgaccgcc
     2221 gcgatcagaa tgagggcggc aatatctgat tttcttctta aaaattgtat cagaaaacca
     2281 gatgcgttct ggagttgtga tatggatttt ccggccatat gagacggtat aggaaaaaca
     2341 tgtttgcctg tcaatgataa actcgctgtt gaatccggca gcgagcgtat ccaacgatac
     2401 ctgcggcggg gtcggcgagc gacattaggt aagtacgtca tttcctatgg atcgaagact
     2461 ttccagcgtg cgctgtgaaa agcttccatt tattacgttc ggtttgccgg atgatgtttg
     2521 gcgggctttt agtcttgagc gaatggagga gcgtcgggga tgatcaagcc aggcaattca
     2581 tatcagaaga tatcaggaaa aaattttttg aacatcgata attctatatt gaatttgaaa
     2641 aacaggttct ttaaacgcca gcgggcaaca ggacgataac actttgccag aaaaaccaaa
     2701 agtttgtttt tggaaaagtc agaaacttta tcgtcatcga gcaggctggt taaaaacagc
     2761 gactgaaaaa aatgcgtatc ttttagataa tcggcgaagc tgtttgtgtg ttctctttcc
     2821 catcccacct gaccccattc ttccagtgat tcgggaccgg ggaaaacatc cttgtactgt
     2881 tcatacagcg gtgttccgga aaaagggatg tacatataga aggggctgtt gcggacattt
     2941 gggttgatgt gctgcaaccg gaaaatcagt ttgattgatg cgcggagatc atcgagggtt
     3001 tctccgggca aactgataat atagctgcac cagatcagaa tttttgtccc gctcaatctg
     3061 gccataacct gttcgatatc ggccgggctt ccctgtttgc cgattaacgt tcgcatcttc
     3121 ttcgacgccg attcaacccc aatggtgagt cttgtcaaac cgcacgtttc aagacgtttg
     3181 aagtcgcttt cggtgtagtc tttcagcgca atgatatcgc ttccctgaac cgcccagcgc
     3241 aggccaagct cgcccagccc ctttccgaga ttcatcacat agtccttgcc cattgcgaat
     3301 atgttgtcgt caacaaaaaa gattcccgtg aaagaagcgt atctctcagc cagagttttg
     3361 atttctgaaa tgattttatc cgcagatttt gcacggaatt tccctttgtt gaaaacatga
     3421 ttatagcagt accggcaatg tcgcggacac ccccgcgatg cctggtaata agcgtatttg
     3481 tgaccgttgt aatattgcac gtaattgttg aaatccacca gatcgtatgg aatgacaggg
     3541 gcggcctgta aatctattaa atctctgtct cctccgtaag caatctttcc ctgctttttg
     3601 atccagacgc ctctgacggg cggcatattc tggccgtatt ggacaagcct gtcggaaaat
     3661 tcgggaaggg tgagttctcc ttctccctgg atgacataat ctatatactg attttccagt
     3721 gtttgttccg gtaataaaga cgggtggatg cctccccaga caacggggac ggatgattgc
     3781 tctttaatat accggctgat ttccagagca aatttaatca tcccgccgct gtaaacggac
     3841 agtccggcat agagaggctg attttgcagc acattgtaaa gttttgcgta aaaagagtcg
     3901 tcggaagcaa aaagacgctg atccagaatg attatctcgt aggtatcttt caaaaacgac
     3961 acggcagcga ggattcccag cggcggtgca ggtttgctgc gttgggcatc catgtatcct
     4021 aatcgagggt agataaaaag tattttacgc accgacagct cgctctcttt tcaaaaccgg
     4081 ttttaacctt gcataacagc aaccgtcgca gattgcaaag agacttcttt tttttaattg
     4141 gtttctaaac cgaatatttt ccggcgacgt aagaacctgg gcaagcgatt gtttctttaa
     4201 atcccctatt ctgacggaca agcaggggta ggcttctccg gtggaactta tgaccattcc
     4261 tgaccacggc atacggcaag cggaatatct tttttggatt gcagacgttt caaaatagtt
     4321 tttcagatct tccgcctgtt tcatctgcgg atatgttctg agtctggtcc gggaagttct
     4381 ggctatggag aataattcct tataaatgtc ttcgatgttt tccgggaagc ggtagttccc
     4441 gtttgatacg ggttcgcaat caagcactgc gatgtcgtgc agacagtcgt tgaactgaaa
     4501 ttcatttttt ttggggaagg acactgtgaa tgaatcagcg ccatgatcgg cagcctgctg
     4561 gtaaagaagc gggaggcggc tgacattttc gggaaaaacc gttactttcc agtcaaatgc
     4621 cggaaacgaa cttttggctt ttttcttgtg ataggccaga cggtctacat tatccatcac
     4681 aacagcataa ttgccccgcc cctttatttt ttcataatag tatttatcac cgtcaatcgc
     4741 gatgccgata ttcagcaatc tgttttgaat catttcgcaa atgatatcgt cgttcaacag
     4801 cagtccattt gttaaaagcg ctgtttttct ctttagacgg cgggtttctt gcaatagctg
     4861 agtaaaatcg tcccggcaaa gaggttctcc tccggagaac acgataaccg catatcgcgg
     4921 taatttctta ataatgccgg ctatgtcttc aaaggatagt tcaggccgat tccgggtcag
     4981 gctcgtcttc tgacagcagt agtaacagtt taaattgcat tgattggtga tattaaaaag
     5041 atagaggaat ggttttaatg cagtttgaag ctgcgggatg tcatacggaa aacgcggcca
     5101 gatgttgcat gctaatccat acaggtttga taaatcgaac aaagaattca taatattact
     5161 tattttcgaa ggacaaaatt attaaacatt agattcaatt tatatacgat gaataaaatc
     5221 tctcatcgta ctgcttacac cagggacgta tcttcttctt tgagtcgacg gaaagatacc
     5281 acgttattca ggcgcgtcca cgcgcttgca ggaatatcgg caagggtcgg cctgtcttgc
     5341 gctatacgcc agaggcgcac cggattgaaa tagaaaccgt aatacgccca gcggtaaagc
     5401 atccgatagc gccggtctga aacatcgctt ccgttgaaag gcgctccgta atattcatag
     5461 tcaatgcttg tggacgttga agggagtttg cccgtatcga cgaaaagatc atagagcgct
     5521 gtattgcgga aaggattggg cgtaaaaaac agtgccatgt gcaatctgga ggcatgcgca
     5581 aaacggattg tggcaagcat ttcatctttc gtttccgtcg gaaagccgag catgaagaaa
     5641 ccgcgagtga aaatgcgccg gtccgcaaac cagtcaatgg tgcgcttcgc tttttccaga
     5701 gagagatttt tgcccagcag tttttgtagc ctatccgatg cggtttcaat cgctacggaa
     5761 atctccccgg tgccgacaca tttcagcagg tcggcggatt ctttacgaat taaatcggcg
     5821 cgcaatgcat tggggaaact caacacggga tgcagatttt tttccagcat taattccagc
     5881 attctgtcaa aacgttccgg gttaaaattg gatatatcgt cgagaatctc tatgctttcc
     5941 gcgcccattc tcatcagccg tgctgtttct tcaacgacgc tttccggtga ccggctgcgg
     6001 aaggacttgc cgaaaagttg atggcaataa atacaattat agggacaacc gcgcgacgtg
     6061 aacatcgtca gataaggtct cacaccatcc gttgccattt tggggagggt ccagtatttt
     6121 ttgtaatcga tcaggtccca ggcgggaaaa ggcagagcgt cgagatcgtt tatcggcgcg
     6181 cgcggttttg aaagcttcat ctttccgtca acgcgaaaag cgagaccgtc aatcttctgc
     6241 aatatgtccg gattttgcca gacggatttt tcagacatgg tgacactgac cagttcagtg
     6301 aatgtgtctt ccccttcgcc catcacaaca gcatccacgc aggaatcctg caaaacaagt
     6361 tccggatcgc aggatgcatg aggtccgccc atgatgaccg gaacggacgg tgcttccgcc
     6421 ttgacggcag cggcagcttt gtgcgcgaaa aaagcctctg cggtcaaagc gcttattccc
     6481 acgacatcag gacgaaagtg tctcaacgtc gataccatca cgttcatcgg gtcgttctca
     6541 agcatagcga cgatcacgcg cacatccgct cccagccgtt cccgcagagc ggcggcgata
     6601 tagagtaatc ccagcggcgg cgtcacgcct gtgacttttg aaacaatgct gtggcttttg
     6661 aaaagaagta ttttaggtcg tttcatcgtt catcttccaa gacaacccat cgcaaacttc
     6721 gatgaagttt cctgaaatgt gtatgacgaa tgtaaaatta tctcaaccat tttttaaatt
     6781 gacgatttta tttcgagata tgaataaaat tcatcaacgg cagtcccctt tgctttcggc
     6841 aagttccagt aatttacagt ctcccagcgc acaggccttt ttaaaatcag agcacccctg
     6901 ttctttctcg ccctgtaata aataagcaat gcctctgttt ttataagcat cgggataacc
     6961 cgggttaagg cggatggatt cgttaaagtc tgcaacggca tgttgatagc ggccgagttc
     7021 gctgtaggtc gttccccggt tgtgataggc attagcgaaa tcctttttaa agtgaatggc
     7081 gtgagtaaag tatcctaacg ctatccgata ctgtccacgc ttgaaataag agatgccgct
     7141 gttgtgatag tatccgctgt cgtgtggctg aagatccgct gccagacatt ctcctttgcc
     7201 cagtgccata tccagaagtt tgcagttgcc caggtcgcat gccttttgat aatcaaggca
     7261 tgcgagttct tggccgccta aattcgaata aacgatgcct cgggtcagat ggatatcggg
     7321 cgcgtcaggt tctcttcgaa tcgcttcgtt caaatcatca atggcgcgtt ggtaccggcc
     7381 catatggata ccgtaagtaa agcctctatt gtaataggct ctggcataat caggattcag
     7441 gcgtatggcc tcgctgtagt cgcttatggc ctgttgatag cggccgagtt ggagatacgt
     7501 attccccctg ttgttgaagg caaaagcata gtccggtttt atgcggatgg cctcgttgaa
     7561 atccgcaatg gcaggcggat actgttgcga cttgctgtac acattgcctc tctggttgta
     7621 cgccagataa ttatcttttg tgacatgcaa agcatgattg aaaagcttga aactgttttg
     7681 ccagtaactg cattgttgcc aggcaagaac cgacagcatt gccgcaacgg cgatcccggc
     7741 cggccataag atctttctcc ggatttgttt ttggcaaaac agatccggaa tgccccaggc
     7801 cgccatcaca taaaggccta cagcgggcag atacgtgtaa ttatcggaca tggcccgcgc
     7861 actggacctg aagatatcaa gcaccggcag aacagcaagg acataccata accatccgac
     7921 aagcaaatac ggcagacgtt ttgccgcgag gatgacggca acgctgatgg ccaggatcag
     7981 cgctattgat cctgcggtct gccaggcggg caactgatag gaaaaaggat agaagacggc
     8041 cagatcataa ggccagaggg tttttgccag ataggtcgtc agcgaaacag gcgcgttggc
     8101 caggcgggaa tcgaatgatg tctcggcatg ttttatgtat ttgtcgtact gggcgaagat
     8161 tgtgatgagg gaaaaaacgg cggataacat gaaaaaacgc aatttttcct tgacctgcca
     8221 gaaaacggga ttttcttttt tcgaggcaaa ccgtttcagc ggccagtaat ccaaaactat
     8281 catgatcacc ggcagggtaa ccgccatggg tttgctcatc agcgcgcagg caaaggacag
     8341 gaaaacgaat agatagcgtt tgaccattgg cttttccgtg taataaacat acaggcacaa
     8401 ggtcagcatc cagaaaaaag cgctcaggac atccttgcgt tttgctatcc agacgacggt
     8461 ttccacatga agcggatgaa tggcgaacag agcggcaaca aaagcgcttc tccaaacggc
     8521 tcccgtcatg cgatgcaaga gccagaacag catgagcgtg ctcaggatgt gcagcatcag
     8581 gttggtgatg tgataccccc cggcgttcaa cccgaagagt tggtaatcaa gcatgagcga
     8641 aagccaggtc agcggatgcc agaattcggc atggattgtg ccgaaggccc agatgacacc
     8701 gtccatcgtg aaccccgatt gaatagacgg gttgtcaacg acgtagaccg gatcgtcaaa
     8761 tgttataaaa tcaaattggc cgacctgcga gaaaacaaag agagacaacg ctattaaaac
     8821 gacataaacg atcagtcttt gtctgtcgga gcttaaattc atttttttca gcatggtgtg
     8881 aagcggtaac cccatttgcg caaaaaagaa atttcatatc atgccgcgaa cgtgcaacag
     8941 tgccagcacc ccgtgacggg tgttcagagc ttttactaca tgttgattca gaattcagca
     9001 gttttttgaa ttattctcca ttgacaaata gacaacacct ttctatattt cttttacctg
     9061 agaaaatatg agcattcaaa aacatcatgt gtgggaatat ccaactctct caacgcctaa
     9121 agccatattg agatttgtct ttacctggct ggtcaccctt gccatctttg tcgttctctt
     9181 ttcccgaatt ccgttccatg atgtcgtgtc ccttcttcaa caaacggata tgcgattcct
     9241 ctgtgcgggc ctcctttgtt ccgtgctcgc ccatttattc ttctcctcgg caagatttca
     9301 acgtgttgtc aaaatgacgg gatgccggat ttcatttggc gaagccgtga tcatccgaat
     9361 gggctgcaac cccatcaaag gcattctccc gtttaaaatg ggggaattcg cgattctggg
     9421 ctatatgaaa gaaagacatc atctctctta cgcccgggga cttcttgcgc tgttatcggg
     9481 ttacatgttc agtttcacgg cgttgatttt tttctgctca atgggtggtt tattttatgt
     9541 tcatcctccg cttcaaaaaa tgctctttgc cgctgcctgt ctgctgatcg ttttactgat
     9601 tgcaatatca atccggcaaa atttttttcg ccccgtcgtt ttatatattc ataaaattct
     9661 gagattaccg gaaaacgtca gaaaaatcat tgaaagttat gacccgcaaa caggtcaagc
     9721 gatcatgatc cactctctgg caatagaagg ttgcaagttg ctcattattt tgttttcatt
     9781 caaatccctg tccatagacc ttcccgtcga tgccctttta ttatggggat ccatgaccat
     9841 tattgccgcg tatctgccca tgacctactg ggggttgggg atccgggaat ctgcggtcat
     9901 cgtacttttc gccggttatg ccgctccggg tcacttgctg gccgcgggcc ttctgatgac
     9961 ttttgttgac ggtctgcttc cggttttgct cggcctgttt tttatcagac cgtttttgaa
    10021 taattggctg ggaagcatga tgcaaaaata atccacggga cacgcggaaa gaaaatatgt
    10081 cgggaatgcc ttgttgaatg atatagtaca tgttattcat gaaaaaagtg tagaggaaaa
    10141 atgagactgg cgtgcgtttt caatccattt caatataagc tgcatgaaga aaacctgaga
    10201 gtggttcaaa aatatttcgg gcttttcccg cctctctctc tggcctgggt ttgcgcgatc
    10261 gccgagaaag ccggacacga cgcgctgctg atcgacgccc gcacgctgcg tttgaccagg
    10321 gaagacgtcc tggaccggtt gaaacagtgg aaaccggaca tcctgggttt catgatgacg
    10381 acctatatgt tccgtgaaac gttggaatgg gtgacgtttc tcaaaaaaca tcttaagatt
    10441 ccggtaatca tcggcggtta taatctgagg gtttacccga acgagtccct gtcggtagag
    10501 gggcccgatt tcggctgctt caacagcgct ttttacaccc tgccgcggtt gctggaggag
    10561 ctggaaggcc ggcgccggtt cgccgatgtg cccggtctga tttacaaaga aaacggtcgg
    10621 acaacgatga ccccgtacgg cagggatccg gttttcgacg actacccgaa tcctgcgcgc
    10681 catcttttgc ccaatgacct ttacgccgaa tttacaacgg agcgcaagaa cttcaccgtc
    10741 atggtgacga gcaaaggctg tcccaacggc tgtttgtttt gcgaagccgg ccacacgtcg
    10801 tacaatccgc gcagtcccca gaccgtcctg ggcgagataa aggaatgccg cacccggtac
    10861 ggcattcgtg agatcgattt ttttgattat gatttctgca tcgaccgcaa acgaaccgaa
    10921 gccatttgcg acgggattat tcaaaatggc atggatatta cctgggcgtg ccgcacccgg
    10981 gttgattgtg ttgatgaagc cctgctgcgg aagatgaagg cggcgggatg ctcccgcata
    11041 tacttcggca tcgagtcggg tgtgcaggaa atcctggatc gtctgaacaa agggatcagc
    11101 cttcaacaga tccgcgatac gattgaaacc gcccggagcc tgggaatcag agcgcttgga
    11161 ttttttctcg tcggagcgcc gggtgaaaca cgggagacgt ttcaacaaac ggtaa
//
LOCUS       DAOSME010000066        10995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6806_length_10995_cov_19.954845, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000066 DAOSME010000000
VERSION     DAOSME010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_10995_cov_19.954845"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLG98_09155"
     CDS             <1..947
                     /locus_tag="PLG98_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE33467.1"
                     /translation="LLAFATVAQAEVKAGSFSVTPFIGGYVFEGNEYDNNEDTYAAGL
                     RAGYNFTKNIGVEAFFNYVPTNNKDWNDDNEMELFGYGLEGLYHFMPESRFVPFFAVG
                     VGGIHYDALPEDPDNRDKFALDYGAGFKYFVTDNIALRADVRHIIPFNERYNNLLYSV
                     GLTFAFGGEKKIVETKVKEPLPPPPPPAPVAKPVIIEKGKQTLDVKFDFDKATIQDGY
                     YKDIDALVEVMKDYPELNVVIEGHTDNVGKAEYNKKLSQKRAETVKKYMVEKGGVDAA
                     RIEAKGFGDERPVDTNDTADGRAKNRRVEAAVDYIIEK"
     gene            1382..2392
                     /gene="aroF"
                     /locus_tag="PLG98_09160"
     CDS             1382..2392
                     /gene="aroF"
                     /locus_tag="PLG98_09160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE33468.1"
                     /translation="MIIVMKSHATEKQIEDVIHWIESVGYKAHPSIGVERAIIGAVGD
                     NRGKDILKFAESLPGVEKTMPILKPYKLASRESHDGNTEVTVGEVTVGGPEFVVMAGP
                     CAIESEEQLLESAYVVKKGGAQILRGGAYKPRTSPYSFQGMEEEGLKVLNKVGTQAGL
                     PTVTEVVNPADVDLVESYADMLQIGARNVQNFALLKKVGHSRKPVLLKRGMMTTIEEL
                     LMSAEYILSEGNPNVILCERGIRTFETATRNTLDISAIPVLKGLTHLPVIIDPSHAAG
                     NWKYVIPLSRAAVAVGADGIIVEVHPEPEKAVSDGAQSLKPEKFYQMMDEIRAITAIM
                     KH"
     gene            2423..3517
                     /gene="aroB"
                     /locus_tag="PLG98_09165"
     CDS             2423..3517
                     /gene="aroB"
                     /locus_tag="PLG98_09165"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOE33469.1"
                     /translation="MRSIKVNLDRKSSSSYEIRIGKDISDRLSLLIAKNHKAARYAVI
                     TDNIVGELYGKKFLTALKEIGLNASLIDFPAGESSKTIKTVVDIAETLAVSGADRETC
                     LIALGGGVVGDIAGFVASIYMRCVPYIQIPTTLVAQVDSSIGGKTGVDLPCGKNLAGT
                     FYQPQAVMTSLHFLNTLPPKEFNNGLAEIIKYGVIDDEKMFASLEDIMTAVKSKDPKI
                     LLGVVEKCCQIKKSIVEIDEKEGGVRRILNFGHTIGHSLEAVSGYAITHGEGVAIGMV
                     AAARLSARMGYLKNNEAGRIETLIDQAGLPTRIPKTLSGGKIVAQLKMDKKRKGAAIH
                     FVLLKKIGLPFIHGNIEQKMIADVIEDMKS"
     gene            3514..4596
                     /gene="pheA"
                     /locus_tag="PLG98_09170"
     CDS             3514..4596
                     /gene="pheA"
                     /locus_tag="PLG98_09170"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOE33470.1"
                     /translation="MKPHLLENLRKKIKDKDKEIVRLLNERAQISVQIGKMKGQGGMQ
                     VYDPAQEAKVHGYLRDLNAGPLASGAVSAIFREIISASRDLQKPMTIAFLGPEASFSH
                     LASRLHFGESSLFFPQTGIFRVFDEVEKGAIDWGVVPVENSLEGSVNVTLDRLMITPM
                     KIRAEIYLRIRQCLMSSAKNMKEVKRIYSHPQALAQCQVWVRTNLPNCVVCETESTAA
                     AVQMIRGKKHEAAIGSSLAAKIYGVNLLAEGIEDNPSNMTRFLVIGQGANDPTGNDKT
                     SLIFATPHSPGALHAALSAFARRKINLAKIESHPVKDKLWEYSFFVDMIGHADDRRVQ
                     GCLRELQNKTSFMKILGSYPKSEDAL"
     gene            4593..6167
                     /locus_tag="PLG98_09175"
     CDS             4593..6167
                     /locus_tag="PLG98_09175"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOE33471.1"
                     /translation="MICIPITSDNPKEALHAIERSCQLADFLELRMDLIGNIGLAELI
                     SAVRAESAAVKIIVTSRKKEEAAFVGRWPRRRNACSKTRYKIALLKEAVERGADYIDI
                     ELAEGTQAIRQLQALCAEKGSQTKMIISYHHLKDTPDLATLKKIFHRCAAYKPAVVKI
                     VTTARSEEDNLIILNLIAYARRKSQKIISLCMGEKGGISRAVAPLMGNFLSFAALDRT
                     GRSAPGQFTVHEMNRIQKWFKGKNRAPQSSASSPHASLPRHYVLLGNPVEQSLSPVMH
                     DAALKKTGLAEQYVAFCVQDIGGAMDGLRAMNIHGASVTIPFKVAVMEYLDDIDADAL
                     EIGAVNTIVNENGRLIGHNTDWAGLILTLKKAMTIRHKTIVIVGAGGTARAAAYGILK
                     EGGCPVIVNRTPKKGKMLAEKMQCAFYPLSEIGRIRGDVLINTTPVGMYPRGNQSPVS
                     AAVLSDYDCVMDVIYNPIKTKLLRDAEKQGCRIVTGVEMFVNQGAEQFRLWTGKEPPH
                     AVMKKIILERLSKGGR"
     gene            6157..>6503
                     /locus_tag="PLG98_09180"
     CDS             6157..>6503
                     /locus_tag="PLG98_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33472.1"
                     /translation="MDDNPIRCPDPAVTVRVPGSKSLSQRALVAATLAQGDSMISNLL
                     VSEDTLYMIGALRELGAKIVDAPDGFQVTGTAGKIENSGRDLFLGNNGTALRFLTALV
                     CLGKGQYVLTGEK"
     assembly_gap    6504..6603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6604..7568
                     /gene="aroA"
                     /locus_tag="PLG98_09185"
     CDS             <6604..7568
                     /gene="aroA"
                     /locus_tag="PLG98_09185"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOE33473.1"
                     /translation="TALVCLGKGQYVLTGEKRLCERPVGALGDALRDMGVDIRTHHHC
                     PPVTINAGGLAGGKITLRDIESSQYVSALLLCAPYTDRGIELSLEGGVVSAPYIDLTI
                     AVMEDFGVTISKPERFAYRVNAGEPYRGRRYVVEGDASSASYFFLAAALLKTTIRVEG
                     ISRESRQGDIRLLDVLEKLGCRFRSTTNGVDVTGGTLLKGDRVFDFGDMPDMVPTLAV
                     LSAFREGQTVINNVAHLRIKESNRLAAVATELRRAGIDAREIPDGLVIKGGRMRPAKI
                     ETYNDHRMAMSFAIAGLVVPGIDIRDKKCVDKSFPSFWEELRRL"
     gene            7565..8137
                     /locus_tag="PLG98_09190"
     CDS             7565..8137
                     /locus_tag="PLG98_09190"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOE33474.1"
                     /translation="MKVVLIGYRATGKSTVGKMLAARLNVDFRDTDAMIEESMDMPVK
                     EIVALHGWEYFRSKERDIIKSLVKKEDCVIATGGGVVLFRENVDLLKQNVDLLKQASV
                     MVWLNAPLRDIVERLKQDAQNEATRPPLTSGNIVQETIDTMRKRFPLYERSADYVVDT
                     LSKTPEQITEEIYQYLLKSGNLAKITVIKK"
     gene            8149..9225
                     /gene="aroC"
                     /locus_tag="PLG98_09195"
     CDS             8149..9225
                     /gene="aroC"
                     /locus_tag="PLG98_09195"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOE33475.1"
                     /translation="MSGNTTGSVFRVTTWGESHGRALGAVVDGCLPGIPLTESDIQQA
                     LDRRKPSASVASTTRKEGDLVEILSGVFEGKTIGTPISLIIRNADAQSSSYDALKNVF
                     RPGQGDFTYLQKYGIRDWRGGGRASGRETAARVAAGAIAAKILDRVGIDVIAYTKAIG
                     SIAIDRGRMAAEKKLRTAVQENALFCPDLQAAARMERKITAVRKKGDSLGGIVEIIVR
                     GCPGGLGEPVFDKMDADLAKALMSIGSVKAVEVGDGFSMAGLQGSQTNDEIMPGGFKT
                     NHAGGILAGITNGEHIVLRAYCKPIPSIAKPQRTIDSKGKPRFIEIQGRHDICVLPRI
                     VPVCEAMVNIVVADHFLRQRAIQS"
     gene            9222..10007
                     /locus_tag="PLG98_09200"
     CDS             9222..10007
                     /locus_tag="PLG98_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOE33476.1"
                     /translation="MKKINVGIIGGTNGMGKWFADLLKKEGCRVYVCGRKTRLHINDL
                     AGLCDVVVVAVPISATPEVIGQVGPQLTKDQLLMDLTSLKKEPVTRMLKTSQAEVIGC
                     HPLFGPQLKDVSGQNVILCPARGKKWLGWLKGVFKRSKMAVRESTPEKHDRMMAVVQA
                     LNHLDTMVLGLAIARTGMAFADIDKFSTPIFRIKMEIIRKVFTESPELYLDIIRGNSQ
                     TIKMLNIYEKALKDIRAVIRTRGKKESTEFVTKVAAKVHGRKN"
     gene            9991..10758
                     /locus_tag="PLG98_09205"
     CDS             9991..10758
                     /locus_tag="PLG98_09205"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOE33477.1"
                     /translation="MEEKINLVAAMIAASKKTVIFTGAGISTESGIPDFRGPDGLWTK
                     VDPEDFTIDRYLRSGETRKKVWYYLVEGGLMTTSQPNRAHLAIAELERMNKLSSIITQ
                     NIDNLHQRAGNDPKKVHELHGNMQWLVCLDCAERYDLEMMRQKHPSPDHFPICEKCAG
                     LLKPGVVFFGEMLPPDTLRMAEQESEKCDLFMVIGSSLVVYPAAYMPLYAKQSGAKIV
                     IVNMGQTGQNDIADVFINAPAGDTLSRIVARLKEIMT"
     gene            10781..>10995
                     /locus_tag="PLG98_09210"
     CDS             10781..>10995
                     /locus_tag="PLG98_09210"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOE33478.1"
                     /translation="MCLIVFAYNVHPSYRLVLAANRDEFYERPSSPAGFWKDHPRMLA
                     GRDLKSGGTWLGITKDGRFAAVTNYRDP"
BASE COUNT         2865 a         2502 c         3055 g         2473 t
ORIGIN      
        1 tgctgttggc ttttgccaca gtggcgcagg ctgaagttaa ggcaggttct ttttcagtaa
       61 cccctttcat cggaggctat gtctttgaag gtaatgaata tgataataat gaagacacct
      121 acgctgccgg tttgcgcgcc ggttacaatt ttacaaaaaa tatcggtgtg gaagcatttt
      181 ttaattatgt tccgaccaat aacaaagact ggaatgacga caatgaaatg gaactcttcg
      241 gatacggtct ggaagggctt tatcatttta tgcccgaaag ccgctttgtt cccttttttg
      301 ctgtaggggt aggtggtatt cattatgatg cgcttcccga ggatccggat aaccgggata
      361 aatttgctct cgattacgga gccggcttca aatatttcgt tacggacaat attgcgttga
      421 gagccgatgt ccgtcatatc attccattta acgagcgata caacaacctg ctttactcgg
      481 tcggcctcac cttcgccttt ggcggcgaga aaaagattgt agaaacaaag gttaaagagc
      541 ctctgcctcc tcctccgcct cctgctccgg ttgccaagcc ggtcatcatt gagaagggaa
      601 aacagaccct ggatgtcaaa tttgattttg ataaagcaac cattcaggac ggttattata
      661 aagacatcga tgcgctggtt gaagtgatga aagattatcc ggagttgaat gtggtcatcg
      721 aaggacatac ggataatgtg ggaaaggctg aatataataa gaagctctct caaaaacgcg
      781 ccgaaactgt taaaaaatac atggtcgaaa aaggcggtgt cgatgccgcc cggatcgagg
      841 caaaaggctt tggcgatgag agacccgtcg atacaaacga tacggccgat gggcgtgcga
      901 aaaaccgtcg cgtggaagct gcggttgatt atatcatcga aaagtaaaga aggattataa
      961 atcgtttcaa cataaaagag ccccggggat cacttttcgg ggctccttta tatttcttct
     1021 tcatgacaat gcaggcttga aacctgatcg gaaggatggc ctgccgcagt aagacgcccc
     1081 tccctttttt catcacgacg atcatgcaat aaaaaaaggc ttgacaaata gtgtcaggtt
     1141 cattagaaag cgtacaaaca aagacaggaa tctcacgatg gacagaaaac agaatgtaaa
     1201 tatgaatgtt tgcggttact ggttttatac ctattttata tcggtctgcc cgtcgctgag
     1261 gaggactcgt taaggttcag taaaccaaaa cagccatggg cggatcgaaa gcgctcatgg
     1321 cttttttttt agaaagccag ggcgtgcaga cccctggctt tttaatttca aaggaggcaa
     1381 tatgattatc gtcatgaaaa gtcatgcgac cgaaaaacag attgaagatg tgattcactg
     1441 gattgaatcc gtcggataca aggcccatcc ttccatcggg gtggaaaggg cgatcatcgg
     1501 agcggtgggt gacaatcggg gtaaggatat tctgaaattt gcggaatccc tgcctggtgt
     1561 ggaaaagacc atgcccattt tgaaacccta caaactggcc agtcgggaat cgcatgatgg
     1621 taatacggag gtgacggtag gagaggtgac cgtcggtggc ccggaatttg tggtcatggc
     1681 cggaccctgc gcgattgaaa gtgaagaaca gttgctggag tcggcctatg tcgtcaaaaa
     1741 aggcggtgcg cagattctga gaggcggtgc ctataagcct cgcacgtcgc cttacagctt
     1801 tcaggggatg gaggaagagg gcctgaaggt cttgaacaag gtcggcactc aggccggttt
     1861 gccgacggtg acagaggtcg tcaatccggc ggatgtcgat ctggtggaat cttacgccga
     1921 tatgctgcag atcggcgccc gcaatgtgca gaatttcgcc ctgttaaaaa aagtcggtca
     1981 ttcgcgaaaa ccggtgctgt tgaagcgggg tatgatgacc acgattgaag agttgctgat
     2041 gtcggcggaa tacatactct ccgaaggcaa tcccaatgtg attctgtgtg aacgcggtat
     2101 ccggacgttt gaaacggcca cgagaaatac gctggatatc agtgccatac ctgttttgaa
     2161 aggattaacc catctgccgg tcatcatcga ccccagtcat gccgccggga actggaaata
     2221 cgtcattccc ctgtcgcggg cagcggttgc tgtgggtgcg gacggcatta tcgttgaagt
     2281 tcatccggag cccgagaaag cagtttccga cggcgctcag tcactcaaac cggaaaagtt
     2341 ctatcagatg atggacgaaa tacgggccat taccgcaatc atgaaacact agaagaacat
     2401 tttatagtga aggaaagaca tcatgagatc gataaaagtt aacctggaca gaaaatcatc
     2461 ctcctcttac gaaatccgta tcggaaaaga catatcggat cgtctgtcgc tgctgatcgc
     2521 caaaaaccat aaggcggcac gttatgcggt cattacggac aatattgtcg gggaacttta
     2581 cggtaaaaaa tttctgacgg cgctaaaaga gatcggcctc aacgcgtcgt tgattgattt
     2641 tcctgcggga gagtcctcaa aaaccatcaa aacggttgtt gacattgccg aaacattagc
     2701 ggtctccggc gctgaccggg agacatgtct gattgctctg ggcggcggcg ttgtcggaga
     2761 tattgccggt ttcgttgcct cgatctatat gcgttgcgta ccgtatatcc agattccgac
     2821 aacgctggtt gcgcaggtgg acagcagtat aggcggtaaa accggcgtgg accttccttg
     2881 cggaaaaaat ctggcgggga ctttttatca gccgcaggcg gtcatgacga gcctgcattt
     2941 tctcaatacg ctgcctccaa aagaattcaa caacgggctg gcggaaatta taaaatacgg
     3001 ggtgattgat gatgaaaaaa tgttcgccag cctggaagac atcatgacgg cggtcaaatc
     3061 caaggacccc aaaatactgc tcggtgtcgt cgaaaaatgt tgtcagatca aaaaatcaat
     3121 tgtggaaatt gatgaaaaag aagggggcgt gcgtcgtatt ttaaatttcg gccatacgat
     3181 cggtcattcg ctggaagccg tttccgggta tgcgattacc catggagaag gcgtggcgat
     3241 tgggatggtg gcggccgcgc gactttccgc caggatgggt tatctgaaaa acaacgaggc
     3301 cggccgtatc gaaaccctca ttgatcaggc gggccttccc acgagaatac cgaaaacgct
     3361 gtccggcgga aaaattgtgg cacaattgaa gatggataaa aagagaaaag gagccgcgat
     3421 tcattttgtg ctgctgaaaa aaatagggtt gccctttatt catggcaata tcgagcagaa
     3481 aatgattgcc gacgtgatcg aggatatgaa atcatgaaac cgcacctgct ggaaaacttg
     3541 cgaaaaaaaa taaaagacaa ggataaggag attgtccgac ttttgaacga gcgggcgcag
     3601 atatccgttc agatcggcaa aatgaagggg caggggggga tgcaggtgta cgatccggcc
     3661 caggaggcga aggtccacgg atatttacgg gacctcaatg ccggaccgct tgcgtccggg
     3721 gcggtgtcgg cgattttcag ggaaattata tcggcctcgc gtgatttgca gaagccgatg
     3781 acgattgcct ttctgggacc cgaggcgtcc ttttcccatc tggcgtcgcg cctgcatttc
     3841 ggtgaatcga gtctcttctt tccacagacg ggaatattcc gcgtttttga cgaggtggaa
     3901 aagggtgcca ttgactgggg tgtcgtgccc gtggaaaatt cgctggaagg atcggtcaac
     3961 gtgaccctgg acaggctgat gataacgccg atgaaaatca gggcggagat atatctgcga
     4021 atccgtcagt gtcttatgtc gtcggccaaa aacatgaaag aggtcaaaag aatttattca
     4081 catccgcagg cgttggccca gtgccaggtg tgggttagaa caaatcttcc caactgcgtg
     4141 gtatgcgaaa cggaaagcac ggcagccgct gtacagatga tccggggaaa gaagcatgaa
     4201 gccgctatcg ggagttcgct tgccgcaaag atatacggcg tgaatctact ggccgaaggc
     4261 atcgaggata atccctccaa catgacgcgt tttctcgtaa tcggtcaggg tgcgaacgat
     4321 ccgaccggca atgataaaac atccctgatt tttgccacac cgcattctcc gggggcgctg
     4381 catgccgcgc tgtccgcctt tgcccgtcgg aaaatcaatc tggcgaagat tgaatcgcat
     4441 cccgtgaagg ataaattatg ggagtacagt ttctttgtgg atatgatcgg ccatgcagat
     4501 gacaggcgtg tgcagggttg tcttcgagag ctgcagaaca aaacgtcttt tatgaagatt
     4561 ctggggtcct atcccaaaag cgaggatgcg ctatgatttg tattcccatt acctccgaca
     4621 accccaaaga ggctttgcat gccatagaac gaagctgcca gctggccgac tttctggaac
     4681 tgagaatgga tttgatcggc aatatcggtc ttgccgaatt gatttcagcc gttcgtgccg
     4741 agtctgctgc agtaaagatt attgtcacca gccggaaaaa agaagaggcg gcttttgtcg
     4801 gaagatggcc gcggcgtcga aacgcctgca gcaaaacgag gtataaaata gcgctgctga
     4861 aggaggccgt cgaacggggt gcggattata ttgacattga gctggctgaa ggaacacagg
     4921 ctatccggca actgcaagcg ttgtgtgcgg aaaaaggttc gcaaaccaag atgataattt
     4981 cctatcatca tctgaaagac accccggatc ttgcaacatt gaaaaaaata tttcatcggt
     5041 gtgccgcgta taagccggca gttgtgaaaa ttgtgacaac ggcgagatcc gaagaggata
     5101 atcttatcat attaaatctt attgcctatg cgcgcaggaa atctcaaaaa atcatttcgc
     5161 tgtgcatggg cgagaaaggc ggcatcagcc gcgcggtggc gcctctgatg ggtaactttc
     5221 tgagctttgc tgcactggac cgaaccgggc gctcggcccc cggccagttt accgttcacg
     5281 agatgaaccg gattcaaaaa tggtttaaag gcaagaacag agcccctcag tcctcagcgt
     5341 catctccgca tgcgtcttta ccccgccatt acgttttact gggcaatccg gtcgagcaga
     5401 gcctgtctcc cgtgatgcat gatgcagccc tgaaaaaaac gggtcttgcg gaacaatatg
     5461 tcgctttttg cgttcaggat atcggcggtg caatggacgg gttgagagcg atgaatattc
     5521 acggcgccag tgtgaccatt cctttcaagg ttgccgtgat ggaatatttg gatgatattg
     5581 atgcggatgc gttggagatc ggagcggtga atacgattgt caatgaaaac ggccgtctga
     5641 tcggacataa cacggattgg gccggattga ttttgacgct taaaaaggca atgacgatca
     5701 gacataaaac cattgtaatc gtcggcgcag gcggaaccgc tcgggcggcg gcttacggca
     5761 tcctgaaaga gggtggatgt ccggttattg tgaatcgtac ccccaaaaaa gggaaaatgc
     5821 ttgccgaaaa aatgcagtgt gctttttatc cgctttccga gatcggccgc atcagggggg
     5881 atgtactgat caacacaacg cccgtgggaa tgtatccccg gggaaatcaa tcgccggtca
     5941 gcgccgccgt attgtcggat tacgactgcg tcatggatgt gatctataat ccgattaaaa
     6001 caaaattgct gagggatgcc gaaaagcagg gttgccgcat tgtaacgggt gtggaaatgt
     6061 ttgttaatca aggcgctgag caattccggt tatggaccgg caaggaaccg ccgcatgcgg
     6121 ttatgaaaaa aatcattctg gaaaggctga gcaaaggtgg acgataatcc tatccgttgc
     6181 ccggacccgg ctgtgacggt ccgcgttccg ggatcaaaaa gcttgagcca gcgggcgctg
     6241 gttgcggcaa cgctggcgca gggggattcc atgatcagca atctgcttgt ttctgaggat
     6301 accctttaca tgatcggtgc gttgcgggaa ctgggcgcaa aaattgtcga cgcgccggat
     6361 ggttttcagg tgaccggcac ggccggcaaa attgaaaaca gcggcaggga cctgtttctg
     6421 ggcaacaacg gcactgccct gaggttcctg acggccctgg tctgtctcgg aaaaggccaa
     6481 tatgttctga ccggcgaaaa aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnntgacggc cctggtctgt ctcggaaaag gccaatatgt tctgaccggc gaaaaaaggc
     6661 tctgcgaaag accggtgggt gcgctcgggg atgccctgag ggacatgggc gtcgatattc
     6721 ggacacatca tcattgcccg ccggtcacga tcaatgccgg cggtctcgcc gggggaaaaa
     6781 taacactgcg tgacatagaa agttctcaat acgtgtcagc gcttctttta tgcgccccct
     6841 atacagaccg gggaatagag ctgagcctgg aaggcggcgt tgtttcagct ccctatattg
     6901 acctgacgat tgctgttatg gaggattttg gcgtgaccat cagcaaaccg gagcgatttg
     6961 cctatcgtgt caatgccgga gagccttatc ggggacgcag atatgttgtc gaaggagatg
     7021 cgtcgagcgc gtcgtatttt tttctggcgg cggccctgct caagacaaca atccgggttg
     7081 agggaatcag ccgggaaagc aggcagggag acatccggct gctggatgtt ctggaaaaac
     7141 tgggctgccg gttccggtcg accacaaacg gagtggatgt caccggggga acactgttga
     7201 aaggcgatag ggtcttcgac ttcggcgata tgccggacat ggttccgacc ctggcggtct
     7261 tgtccgcctt tcgcgaggga cagaccgtta tcaacaatgt cgcgcatctg agaatcaaag
     7321 agagcaatcg cctggcggct gtcgcaaccg agcttaggcg ggccggtatt gatgcccgcg
     7381 aaattccgga cggccttgtc ataaagggcg gacgcatgcg gccggcaaaa atcgaaacct
     7441 ataacgatca tcgcatggcc atgagctttg cgatagccgg tcttgttgtt cccgggattg
     7501 acatccgcga taaaaagtgc gttgataaat cattcccctc gttctgggag gagctgcgtc
     7561 gtttatgaag gttgttttaa tcggttacag ggcgaccggc aagtccaccg tcggcaagat
     7621 gctggctgcc cggcttaatg ttgatttccg ggataccgat gccatgatcg aagaaagcat
     7681 ggatatgccg gttaaggaaa tcgtcgctct tcacggatgg gagtatttcc ggtcaaaaga
     7741 aagagacatc atcaaatcgc ttgtcaagaa agaagattgt gttattgcaa ccggaggcgg
     7801 cgtggttctc ttcagagaaa atgttgatct attgaaacag aacgttgatc tgctgaaaca
     7861 ggccagtgtc atggtctggc tcaacgctcc gctgcgcgac attgtggaac ggttaaaaca
     7921 ggatgcgcaa aacgaagcaa cgcgtccgcc gcttacatcc ggcaacattg ttcaggagac
     7981 gatcgacacc atgagaaaaa gatttcccct gtatgagcga tccgcggatt atgttgtgga
     8041 tacgctgagc aaaacccccg agcagattac ggaagaaatc tatcagtatt tgcttaaatc
     8101 gggaaatttg gctaaaataa ccgtcattaa aaaataaaaa ggaaaaaaat gtccggcaat
     8161 acgacaggca gcgtttttcg cgtcacaacc tggggagaat cacacggcag ggctctggga
     8221 gccgtcgtgg acggctgtct tccgggaatt cccttgacgg aatccgatat tcagcaggct
     8281 ctcgacagac gaaagccttc cgcctccgta gcctctacta cacgaaaaga gggggatctc
     8341 gtggaaatcc tctccggcgt ttttgagggt aaaacgatcg gtacgccgat ctccctcatc
     8401 atcaggaatg ctgacgcgca atcttcttcc tatgatgcat tgaagaatgt tttccgcccc
     8461 ggccaggggg attttaccta tttgcaaaaa tacggaatcc gcgactggcg aggcggcgga
     8521 cgggcttccg gcagagaaac agccgcccgt gttgccgccg gagcaattgc agcaaagata
     8581 cttgatcggg tcggtatcga cgtcatcgcc tataccaagg caatcggttc catcgcgatt
     8641 gaccgcggca gaatggccgc agaaaaaaag ctgagaacgg ccgtgcagga aaacgctctt
     8701 ttttgtccgg atctacaggc ggcagccagg atggaaagaa aaattacggc cgtccgtaaa
     8761 aaaggagatt ccctgggcgg gatagtggaa attatcgtcc gcggttgtcc cggagggctg
     8821 ggcgaaccgg ttttcgacaa aatggatgcc gatctggcca aggccctgat gagcatcgga
     8881 tcggtgaaag cggtcgaagt gggtgacgga ttctccatgg ccggtttgca gggctctcaa
     8941 accaacgatg aaataatgcc cggaggattt aaaaccaatc atgcgggagg aattcttgcg
     9001 ggcatcacga acggagaaca tatcgtcctg cgggcttatt gtaaacctat tccgtccatt
     9061 gcgaaaccgc agcgcaccat tgacagcaaa ggcaaacccc ggttcattga aattcaggga
     9121 aggcacgata tctgcgttct tccccgtatt gtgccggtgt gcgaagccat ggtgaatatt
     9181 gtcgtggccg atcacttcct cagacagaga gcgatccaat catgaaaaaa attaacgtgg
     9241 gcattatcgg cggcaccaac ggcatgggaa agtggtttgc cgacctgctg aaaaaagaag
     9301 gctgccgggt ttatgtctgc ggcagaaaaa cccggttgca cataaacgat ttagccgggc
     9361 tctgcgatgt ggttgttgtc gctgtgccca tttccgccac gcctgaagtc atcggacagg
     9421 tcggcccgca actgacaaag gatcagcttc tgatggattt gacatcgctg aaaaaagaac
     9481 cggtgacgcg gatgctgaaa acgtcgcagg cggaagtcat cggctgtcat ccgcttttcg
     9541 gtccgcagtt aaaagacgta tccggacaaa acgtaatcct gtgtccggcg cgcggaaaaa
     9601 aatggctcgg ctggctgaaa ggcgttttta aaagaagcaa gatggccgtc cgggaatcaa
     9661 cgcccgaaaa acatgaccgc atgatggccg ttgttcaggc gctcaaccat ctcgatacca
     9721 tggtattggg gctggctatt gccaggacgg gaatggcttt tgcggacatc gacaaatttt
     9781 ccacgccgat ttttcgcatc aaaatggaaa ttatccgaaa agtatttacg gaaagtcccg
     9841 aactctatct ggatattatc cggggaaatt ctcaaaccat taaaatgctg aacatctatg
     9901 aaaaagcatt gaaagatatt cgtgcggtta tccggacgcg cggtaaaaaa gaatcaacag
     9961 aatttgtaac aaaggtggca gcaaaagttc atggaagaaa aaattaatct ggtcgcggca
    10021 atgatcgcgg catcgaaaaa aaccgtcatt tttaccggag cgggcatcag cacggaatcg
    10081 ggtattccgg atttcagagg gcccgacggt ctctggacga aggttgatcc cgaagatttc
    10141 acgatcgacc ggtacttgcg ttccggcgag acgcgcaaaa aggtctggta ctacctggtg
    10201 gaaggcggat tgatgaccac gtcccaaccc aacagggcgc atctggcgat tgccgaattg
    10261 gaaaggatga acaagttaag ttcaatcatt acgcagaata ttgacaatct gcaccagcgg
    10321 gcggggaatg acccgaaaaa ggttcatgaa ctgcacggca atatgcagtg gctggtctgc
    10381 ctggactgcg ctgaacgcta cgatctggaa atgatgaggc agaagcatcc ctcaccggat
    10441 cattttccga tctgcgaaaa atgtgcgggg ttgctcaaac cgggagttgt ttttttcggt
    10501 gaaatgctgc ccccggatac gttgaggatg gccgagcagg aatccgaaaa atgcgatttg
    10561 ttcatggtga tcggctcttc cctggtggtt tatccggcgg cgtacatgcc gctgtatgcc
    10621 aaacaatccg gggcgaagat cgtcattgtg aatatgggac agacgggaca gaacgatatc
    10681 gccgacgtat tcatcaatgc accggcggga gatacgctga gcagaatcgt cgcccgatta
    10741 aaagaaatca tgacatgact tccggatggt atgaaacccc atgtgtctga tcgtttttgc
    10801 ctataatgtg catccgtcat acaggctggt tctggccgcc aaccgcgatg aattttatga
    10861 aaggccgtca tcgccggcgg gcttctggaa agatcatccg cggatgctgg ccggccgcga
    10921 tctgaaaagc ggcggaacgt ggctgggcat aacgaaagac gggcgattcg ctgccgtcac
    10981 gaattatcgc gaccc
//
LOCUS       DAOSME010000067        10952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6847_length_10952_cov_25.055612, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000067 DAOSME010000000
VERSION     DAOSME010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6847_length_10952_cov_25.055612"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLG98_09215"
     CDS             complement(<1..475)
                     /locus_tag="PLG98_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33479.1"
                     /translation="MKKIFAKISSMNGVYKAFLVYFLILLLALTYKCFPIFDQSETIN
                     NLSFSHDGKKIVFDRCRNVGCQIQVYDLATGELAAYKSPLTKRWTMGKYSYDGKKITF
                     SIIPIKITGDLNLNKMQIAVMDADGKNFKKVTTGPGAKLYPTFSHNGKKILYARAA"
     gene            complement(612..2024)
                     /gene="selA"
                     /locus_tag="PLG98_09220"
     CDS             complement(612..2024)
                     /gene="selA"
                     /locus_tag="PLG98_09220"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOE33480.1"
                     /translation="MDEQQKELLKQLPKIDEIILLLEKQNIYDLAPHEIVKETCRRIV
                     QNLRDKIVNAKKKLPAEFIPDAKTTAKEVEQSIKGLYRYSLRRVVNATGVILHTNLGR
                     APLCPEALERIMEVGKAYSNLEFDLNKGERGQRYDHVSSLLCALTGAEDALIVNNNAA
                     AVLLVLNTLAQKKEAIVSRGELIEIGGEFRIPEIMQKSATKLREVGTTNRTRQSDYEK
                     AINDKTGLIMKVHTSNYRIVGFTEEASIESLVELGKNRGIPVMDDLGSGCLIDLDRYG
                     LQHEPTIRERLAAGVDVVTFSGDKLLGGPQAGIIVGKKEVLAKIKKNPLNRALRIDKF
                     TLAALEATLMHYLNTSAAPKKLRALKALTEPVADVKKRAEKLIEKLQNENFDQLKFSI
                     REDFAAAGGGSLPTQQIPTVLVAVNNKNMTATRMEEKLRKLEMPIIARVDKDEILFDL
                     RTVAEDEFTFIIEGLKQIIT"
     gene            complement(2127..3377)
                     /locus_tag="PLG98_09225"
     CDS             complement(2127..3377)
                     /locus_tag="PLG98_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOE33481.1"
                     /translation="MINQIVTRIAKREFARSLKFEDGSSPELPSCADNNQRLLYIHIP
                     FCEELCPYCSFHRITFKEPLTRRYFQALRREIRIYHEKGYQFEGVYAGGGTPTVLIDE
                     LAETLNLSRELFPIRDISVETNPNHLTEKNIDVLRQCGVKRLSVGVQTFNDDMLKKIG
                     RYEKYGSGQEIAERLKYYQGHFHTLNADMIFNFPGQTPEMLEADLAVILNLNMDQVTF
                     YPLMISDLTRDIMKKTVGHVDFSNEERFYKLIVRRLEKNYDFSSAWCFSRKESLVDEY
                     VVEFEEYAGLGSGAIGYLYGTCYSNTFDIEGYIASLEKGKLPLQASRKFDLHDRMSYD
                     FLMKLFSTKLDIKAMQKKYDGKFLKTMWKEIAAFEIVRAYRYFDTSLYLTPYGRYLWV
                     IMMREFFIAVNNFRDYCRKQAGVM"
     gene            3653..4213
                     /gene="def"
                     /locus_tag="PLG98_09230"
     CDS             3653..4213
                     /gene="def"
                     /locus_tag="PLG98_09230"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOE33482.1"
                     /translation="MSTKRVLALWDEAGLNARDTSVLRKKSAELPVPLDKEGREAVQA
                     LVDSFMERDDAVGLAAPQIGINKRIIVFRVKGFEDGQKKIKDIDDIEVLVNPRITQER
                     GEKVIGAEGCLSCPDVQVEVSRFPEIKVRALDAKGGKVNKKYLDYIARVVQHEIDHLD
                     GKLIVDYGDIYYPKSKQLFFEKLFKE"
     gene            4701..5792
                     /gene="gcvT"
                     /locus_tag="PLG98_09235"
     CDS             4701..5792
                     /gene="gcvT"
                     /locus_tag="PLG98_09235"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOE33483.1"
                     /translation="MNKTPLYEKHVALKAKIIDFGGWAMPVQYTNVIDEHRTTRNAAG
                     LFDICHMGEILVKGPQALDLLQLVLTRNLADQKIGQVKLSALLNEEGGVIDDLTVYKM
                     DEHDYMLVTNATPKDRDWAWINRIQTDKKFDCALRDISNETGKLDLQGPSSEEILQKI
                     TDADLKNVRFYHFCKAHVAGFDAIVSRSGYTGEDGFEIYAPGNVVGQIWDRLMTEGAK
                     QGMKPCGLGARDTLRLESGMMLNGQDMDESVSPLEVPYGWIVDAQKEFVGSAALRAKQ
                     SSGIRKKLAGLEMTGRGIARHGYKVFCDGKEIGLVTSGTFSPTLNKAIALAFIDIDYC
                     APDTKLEIAIRDTMTAAKVVKLPFYKRLK"
     gene            5809..6207
                     /gene="gcvH"
                     /locus_tag="PLG98_09240"
     CDS             5809..6207
                     /gene="gcvH"
                     /locus_tag="PLG98_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOE33484.1"
                     /translation="MSKPNPNDRYYSRDHEWVIYYNDGTAIIGITDYAQEMLTDIVFV
                     DLPEIGRKVVQHATVAVVESVKSVSDVFAPVSGEVIDVNTVLEETPELINQDAFGDGW
                     IVKMRLSKPEELDMLLTANQYDEMLQEDKH"
     gene            6225..7550
                     /gene="gcvPA"
                     /locus_tag="PLG98_09245"
     CDS             6225..7550
                     /gene="gcvPA"
                     /locus_tag="PLG98_09245"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOE33485.1"
                     /translation="MDYCPHTFDDVAQMQSVIGISSLDELFADIPEKFRLQHIPDIPP
                     ALSEMETLSCMGGLAQKNKMPQVTLTGAGAYHHYIPAVVGHIVGRAEFYTAYTPYQAE
                     ISQGILQAIYEYQTMIARLTGTEIANASMYDGASATAEAAVLCAKMSNRNKMIVARSV
                     HPEYRQVLRTYCWANGYTVVEIPYMPSGQIDPDALQKKLDDQTAAVIVQSPNVFGVIE
                     DIAPAVDAAHQKGALLVAGFTEGTSMGLLKPAGAQGADFVVGEGQSFGNPLNYGGPYL
                     GIFAAKEKYMRRIPGRLVGATVDKKGQRGFVLTLQTREQHIRREKATSNICSNEALCA
                     LAAGVYLAALGKNLRKLAELNVYKARYLKNKLLELPGWQDVFSAPVYNEFVLRCPDAA
                     KVNKKLKKEGIIGGYELEKDYPELKDALLFCATEMISKEDMDKVADIVK"
     gene            7561..9003
                     /gene="gcvPB"
                     /locus_tag="PLG98_09250"
     CDS             7561..9003
                     /gene="gcvPB"
                     /locus_tag="PLG98_09250"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOE33486.1"
                     /translation="MELIFEISKPGRAAAGIPASDVPSINIEQAIGRQYLRDDLDLPE
                     VAEIDLVRHYTNLSRRNFGVDLGFYPLGSCTMKYNPKINENAAALPGFTGLHPYADVK
                     SAQGNLRLLYDMQKYLCAIFGMAEFTLQPAAGAHGELTGVMMIKKYFEKKGQKRRIIL
                     IPDAAHGTNPASSALCGFEIRELKSNGEGGVDIEHLESLMTEDVAALMLTNPNTLGLF
                     ERNIEKVAAIVHGKGGLLYGDGANANAFLGKVRPGDLGFDVIQLNLHKTFSTPHGGGG
                     PGSGPVGVSNALLDFLPVPRIIRKDDKFKWSEKFPDSIGRVRAFYGNFNVIVKAYTYI
                     RSLGADGLKHATEMAVLNANYIKERLKPYFDLPYDRVCMHECVFSGNRQVAQSSVHTS
                     DIAKKLIDYGYHPPTIYFPLIVPEAIMIEPTETERKETLDTFCDAMIAIAREAKENPE
                     GVKNAPVSTPVSRLDEVLAARKPDVCWKKS"
     gene            9078..9941
                     /gene="lipA"
                     /locus_tag="PLG98_09255"
     CDS             9078..9941
                     /gene="lipA"
                     /locus_tag="PLG98_09255"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOE33487.1"
                     /translation="MNPLNEKSSIIRKPDWLKVRPPFSNECRKIRSVLNDLQLHTVCQ
                     EAACPNMAECFASGTATFLIMGNICTRNCLYCNVEHGKPAAIDENEINHLIDAVQKMK
                     LEYVVITSVTRDDLRDGGAHVFADCIRRLRKTVEHCKIEVLIPDFQGNPSALTKVIDV
                     QPDVINHNMEVVKPMFTRLRPQGNYDVSLKLLKNIGPSSVVSKSGFMIGFGENRDDIL
                     RLIDDLAAVSCKRLTIGQYQQPTLKHWPVAKYYHPDEFAEFKEIAYQKGFQHVESGPL
                     VRSSYHAAKAG"
     gene            9999..>10952
                     /locus_tag="PLG98_09260"
     CDS             9999..>10952
                     /locus_tag="PLG98_09260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE33488.1"
                     /translation="MSEKYDLIVIGGGPGGLTAAEMAAKKKKRVLIIEKEGWGGTCTH
                     RGCIPTKALLSCSKYYADLKKLKRMGINIADSSVDFPAVKKHQQQIVKVSALGAQKML
                     NDAGAEIKQGVGEIISPSEVRFTDSSGKSENLSTQNILMAWGSIPQVLSGINLSERIL
                     TSDGVLNLNALPSSIIIVGGSVIGAEFATFFAELGTKVTMVEILDTIIPLEDQECADL
                     LKQELTRMGVVIHTSTKLESLKDTGSSVAMVAQKDGKHLEISADYALLCTGRKPLLFT
                     AELDKCGIEYTQAGIKVDKKMMTSVAGIYAVGDVTGGMMLAH"
BASE COUNT         2865 a         2455 c         2787 g         2845 t
ORIGIN      
        1 gagccgcgcg ggcatagagt atcttcttcc cgttatgaga aaaagttgga tataacttgg
       61 cgccggggcc ggtggtaacc tttttaaagt tcttcccgtc agcatccatg acggctatct
      121 gcatcttatt caaattgaga tcaccggtaa tttttatggg gataatggag aaagtgattt
      181 tcttcccgtc ataagagtac ttccccatag tccatctttt tgtcaggggc gatttataag
      241 cagccaattc acctgttgcc aaatcataga cctgtatctg acagcctaca ttacgacagc
      301 ggtcaaacac gattttcttt ccgtcatggg aaaaactcaa gttgttgatg gtctccgatt
      361 gatcgaatat gggaaaacat ttatatgtca atgccaaaag gagtattaaa aagtacacca
      421 gaaaggcctt atacacgcca ttcatgctgg aaatctttgc aaagattttt ttcatgccgc
      481 ccccccccct gaaatttatt ttacagatac aacgcatgac ttaagttcag tcgagtcttt
      541 tggactcgac tgtgtatttg tgttgtagac agccaagtca aaaagacttg gctgaacaga
      601 gaatgacaat gttaggtgat tatctgtttc aacccttcaa tgatgaatgt aaattcatct
      661 tctgccacgg tgcgcaggtc gaagaggatt tcatccttat ccacgcgggc gatgatcggc
      721 atttcaagct tgcgcagttt ttcttccatc ctggtagccg tcatattttt gttgttgacg
      781 gcaactaaaa ccgtgggaat ctgctgcgtg ggcagggagc cgccgccggc tgccgcgaaa
      841 tcttcacgga tggagaattt caactgatcg aaattttcat tttgcaattt ttctattaat
      901 ttttcagcgc gttttttgac atcggcaaca ggttcggtca aagccttcag ggcccgtaat
      961 ttcttcggcg ccgcggaggt attcaagtaa tgcatcaagg tggcttccag agcggccagc
     1021 gtgaatttgt cgatgcgcag ggcgcggttc aggggattct ttttgatttt cgcgaggacc
     1081 tcttttttgc cgacgatgat tccggcttgc gggcctccta aaagtttgtc gccgctgaag
     1141 gtgaccacgt caacgccggc ggccagtctt tcacggatcg tcggttcgtg ttgcagaccg
     1201 tagcggtcca gatcaatcag gcagccgctg cccagatcgt ccatgaccgg aatgcccctg
     1261 ttcttaccca gttcaaccag cgattcaata cttgcttctt cggtgaatcc cacaatgcgg
     1321 taattgctgg tgtggacttt catgatcaga cccgttttgt cgttgatggc tttttcgtag
     1381 tcgctttgcc gtgtacggtt ggtcgttccc acttcgcgaa gttttgttgc gcttttttgc
     1441 atgatttccg ggatgcggaa ttcgccgccg atttcgatga gctcgcctcg cgagacgatg
     1501 gcttcttttt tctgggccag cgtattgagc accagcaaaa ccgccgccgc attgttattg
     1561 acgattaaag catcttctgc gccggtgagg gcgcaaagca gcgaactgac atgatcgtag
     1621 cgttggccgc gctcgccttt gtttaaatca aattccagat tggagtaggc cttgcccact
     1681 tccataattc tttccagagc ttcggggcaa agcggcgcgc gtcccaggtt ggtgtgcaga
     1741 ataacgcccg tggcattgac gacgcggcgc aggctgtagc ggtacaaccc tttaattgat
     1801 tgttccacct ctttggcggt tgttttagca tcgggaatga attctgcagg aagcttcttt
     1861 ttagcgttga cgattttatc acgcaggttc tgcacaatcc tgcggcaggt ttctttgacg
     1921 atttcgtgcg gcgccagatc atagatgttt tgtttctcca gaagaagaat aatttcgtca
     1981 atcttcggca attgcttcaa caattctttt tgttgttcat ccatcgttat cctcttttga
     2041 tgcgagtttg ctctatcgat tcatttttgt catggggaga catcttgttt ttttgatttc
     2101 tcatacgctt gaagtgacac aaaatatcac atcactcctg cctgcttgcg gcaataatcg
     2161 cggaaattgt tcacggcaat gaaaaattcc cgcatcatga tgacccacag gtaacggcca
     2221 tacggcgtca aatacagaga ggtatcaaaa taacgatacg ctctgacgat ttcaaaagcc
     2281 gctatttcct tccacatggt ttttaaaaat ttcccgtcgt attttttctg catggctttg
     2341 atatccagct tcgtgctgaa tagtttcatt aaaaaatcat agctcatccg atcatgaagg
     2401 tcgaacttgc gggaagcctg caatggcaat ttcccttttt ccagagaagc gatgtatcct
     2461 tcaatatcaa acgtgttgga ataacatgtt ccgtaaagat agccgatggc gccgctgccg
     2521 agtcccgcgt attcttcaaa ctccacaacg tactcgtcaa ccagcgattc cttccgcgag
     2581 aagcaccatg ccgatgaaaa atcatagttt ttctccagac gccggacgat caatttataa
     2641 aaacgttctt cgttgctgaa atcgacatgg cccaccgttt ttttcatgat gtcccgcgtc
     2701 aggtcggaaa tcatcaaggg ataaaaggtc acctgatcca tgtttaaatt aagaataacg
     2761 gccaaatccg cctcgagcat ctccggcgtc tgtccgggga aattaaaaat catgtcggcg
     2821 tttagagtgt gaaagtgtcc ctgataatat ttcagccgtt cggcgatttc ctgaccggag
     2881 ccgtattttt cgtagcgtcc gatttttttg agcatatcgt cgttgaaggt ttgcacgccc
     2941 acggaaagtc tcttgacgcc gcattgtcgc agaacgtcga tatttttttc ggtcaggtgg
     3001 ttgggattcg tttccacgga tatatcccgg atgggaaaga gttcccgcga aagattcaag
     3061 gtttccgcca gttcatcaat cagcacggtc ggggtgccgc cgcccgcata aaccccttcg
     3121 aattgatagc ctttttcatg ataaatcctg atttcccggc gcagggcctg aaaatatctg
     3181 cgtgttaaag gttctttgaa ggtaatccgg tgaaaggagc aatagggaca gagctcctca
     3241 caaaacggga tatgaatgta gagaaggcgc tggttattgt cggcgcagga aggcagttcc
     3301 ggcgaacttc catcttcaaa tttcagtgaa cgggcgaact cccgcttggc aattctggta
     3361 acgatttgat taatcaacga taaatcatcc tcatgatatt ttttataaaa tcaccctttt
     3421 tgttgtttag caaaatatgc gcgtttattg caagcgacaa ttatctccat gtagcttcgc
     3481 acgcgggata atttccaatc ccgataaatc gggatcggat aattcgtccg tcaaatttca
     3541 gtgcgcctat agtgaccttt gggttatttg cttctaacgg tcgggactca tgacttgaaa
     3601 taacggggaa taggtgataa gtgtttttca aaataatttt tgagcaagta tcatgtctac
     3661 gaaaagagtt ttagcattat gggatgaagc aggcctaaac gcccgcgaca cttctgtttt
     3721 aaggaaaaaa tccgctgagt tgcccgtgcc cctggataag gaaggacggg aagccgttca
     3781 ggcattggta gactctttta tggaaagaga tgatgcggtg ggtctggccg cgcctcaaat
     3841 cggtattaat aaaagaataa tcgttttccg ggtcaaaggc tttgaggatg gtcaaaaaaa
     3901 gataaaagat atcgatgata tcgaagttct cgtcaacccg cggattaccc aggaacgcgg
     3961 agaaaaggtc atcggggcgg aagggtgtct gtcctgtccg gatgtgcagg tcgaggtcag
     4021 tcgatttcct gaaataaaag tacgtgcctt ggatgccaag ggaggcaaag tcaacaagaa
     4081 atatctggat tatatcgctc gtgttgtcca acacgagatt gatcatcttg acgggaaatt
     4141 gatcgttgat tatggcgaca tttattatcc caagagcaag caactgtttt ttgaaaaact
     4201 gtttaaagaa taataatgac actcgctgag agcaagcgta gcgcagctcg aagcgttagt
     4261 ggaattatcc ctgaagtgaa gcgaaatggg gtgacactcg ctgatcctaa aactcgttga
     4321 ctataggaaa tgtaagccgc gatcccggga gtggagtgct ctgaagggca tctgctacga
     4381 cacgggattg atacaagatt atttcagtat tcattattga aatatatttt tacttatagt
     4441 aatttttaaa aagcaatcat cacaggtgca atggatatat tcccctgcga aatgattttt
     4501 tccggacaag ccggttttat gctctaaatc cggtatttga agtattgtat tatccttttt
     4561 gcaaggatga aagattcccg gaatctgaca tgcggtcggg ttgtttcagg gatatttttt
     4621 tccggaaatt atagttttca cgggcatgct ttttacctga gtgattgccg tatatttttt
     4681 aaagattgga tgaatcaaaa ttgaataaaa cgccattata cgaaaagcat gtcgccctga
     4741 aggccaaaat tattgatttc ggcggctggg ccatgcccgt ccagtatacc aacgtcatcg
     4801 acgaacacag aacaaccaga aacgctgccg gactttttga tatttgccat atgggagaaa
     4861 tactggtcaa aggaccgcag gcgcttgatc ttctgcaatt ggttttgacc agaaatctgg
     4921 ctgatcagaa aatcggacag gttaaattat ccgcgctgct caatgaagaa ggtggcgtta
     4981 tcgatgactt aaccgtttat aaaatggatg agcatgacta tatgctcgtc accaacgcta
     5041 cgccgaaaga cagagactgg gcctggatca acagaattca gacggataaa aagtttgatt
     5101 gcgcattgcg agatatcagc aatgaaacgg gtaagctgga tttacagggg ccgtcttcgg
     5161 aagagatttt gcagaaaata acagatgctg atttgaaaaa tgtgcgcttc tatcattttt
     5221 gcaaagctca cgtcgcggga tttgatgcga ttgtatcgcg cagcggctac acgggtgaag
     5281 acggatttga aatttatgcg ccgggaaacg ttgtaggcca aatatgggac aggttgatga
     5341 cggagggcgc aaaacagggc atgaaaccct gcgggttggg cgcccgtgac acgttacgac
     5401 tggaatcagg catgatgctc aatggacagg acatggacga gtccgtcagt ccgctggagg
     5461 ttccctatgg gtggattgtc gatgcgcaaa aggaatttgt cgggtctgcg gctctgagag
     5521 ccaagcaaag ttccggaatc agaaaaaagc tggctggcct ggaaatgacc ggacgcggca
     5581 tcgcgcgtca cggttataaa gttttctgcg acggaaaaga aatcggcctt gtcacatccg
     5641 gcacgttttc gcccacgctg aacaaggcta tcgctctggc attcatcgat attgattact
     5701 gtgcaccgga tacaaaactg gaaattgcga ttcgcgatac aatgacggcg gccaaggtcg
     5761 ttaaactgcc attttataaa cggttaaaat aacaataagg agggcattat gtccaagcca
     5821 aacccgaatg acagatatta ttcccgcgat catgaatggg tgatctacta caatgacggg
     5881 acagccatta tcggcatcac cgattatgct caggaaatgc tcaccgatat tgtttttgtc
     5941 gacctgccgg aaattggaag aaaggtggtc cagcatgcaa cagtggccgt agtcgaatcc
     6001 gtcaaatccg tgtccgatgt ttttgcgccc gtcagtggag aggtgattga tgtcaatacg
     6061 gtcttggaag aaacgccgga gctgatcaat caggatgctt tcggtgatgg ctggatcgtc
     6121 aaaatgcgcc tgtccaaacc cgaagaactg gatatgctgc tgaccgcgaa tcagtatgat
     6181 gaaatgctgc aggaagataa acattaaaga aagaaaaaaa cgatatggat tactgtcccc
     6241 acacattcga tgacgttgct caaatgcagt cggtcatcgg catctcaagc ctcgacgaac
     6301 tatttgccga tatcccggaa aaattcagat tgcaacacat tcccgatatt cccccggcgc
     6361 tttccgaaat ggagacactg tcatgcatgg gcggactggc tcaaaaaaat aaaatgccgc
     6421 aggtgacgtt aaccggcgcg ggcgcatatc atcactatat cccggcggtc gtcggtcata
     6481 ttgtagggcg cgcggaattt tatacggcct atacgcctta tcaggccgaa atcagccagg
     6541 gcattcttca ggcgatctac gaatatcaga ccatgatcgc ccgtcttacc ggcaccgaaa
     6601 ttgccaatgc atccatgtac gacggcgctt ccgccacggc cgaagccgcg gtgctttgcg
     6661 cgaaaatgtc caatcgtaat aaaatgatcg ttgcccgttc ggtgcatccg gaataccggc
     6721 aggttttgcg gacttactgt tgggccaacg gctatacggt tgtggaaatc ccttatatgc
     6781 cgtccggcca gattgatccg gatgctctgc aaaaaaaatt ggatgatcag acggccgcgg
     6841 taattgtgca aagcccgaat gtgttcggtg tgatcgaaga tatcgcgccg gctgttgatg
     6901 ccgctcatca gaagggagcc ttgctggtgg ccggttttac ggaggggacg tctatgggcc
     6961 ttctgaagcc cgcaggagcc cagggcgctg attttgtcgt gggcgaaggc cagagcttcg
     7021 gcaatccgct gaattacggc ggaccctatc tggggatttt tgccgccaaa gaaaaataca
     7081 tgcgccgcat tcccggaagg cttgtgggcg caaccgtgga taaaaaaggt cagcgcggat
     7141 tcgtgttgac tcttcagacg cgcgagcagc atatccgccg cgaaaaagcg acatccaata
     7201 tttgttccaa cgaagcactg tgcgcgctgg cagcgggagt ttacctggcc gctctgggca
     7261 aaaatctcag aaaactggcc gagctcaacg tttataaagc ccggtatctg aaaaataaat
     7321 tgctggaact tccaggctgg caagacgttt tttccgcgcc ggtttataat gaatttgttt
     7381 tgcgttgtcc tgatgccgcg aaagtaaata aaaaactgaa aaaagaagga atcatcggcg
     7441 ggtatgaatt ggagaaggac tatccggagc tgaaagacgc attgcttttc tgcgccacgg
     7501 aaatgatttc caaagaagat atggataagg tcgctgacat tgtgaaatag gggatgggga
     7561 atggaattaa tttttgaaat aagcaaaccc ggacgcgctg ccgcgggtat tcccgcgagc
     7621 gatgttcctt ccatcaatat tgaacaggcg atcggccgtc aatatttgcg ggacgatctg
     7681 gatttgcccg aagtggcgga aattgacctg gtccgtcatt ataccaattt gtcgcgccgt
     7741 aatttcggcg tcgatctggg attttatcct ctggggtcct gcacgatgaa atacaatccc
     7801 aaaattaatg aaaatgctgc ggctctgccc ggttttaccg gattgcatcc ttatgctgat
     7861 gtcaaatccg ctcagggcaa tctgcggctg ctttatgaca tgcagaagta cctatgcgcg
     7921 attttcggca tggcggaatt tacgcttcag ccggcggcag gcgcgcatgg cgaattgacc
     7981 ggcgtcatga tgataaaaaa atattttgag aaaaagggcc agaaacgcag aattattctg
     8041 ataccggatg cagcgcatgg gacgaatccg gcatccagcg ctctttgcgg ttttgagatc
     8101 agggaactga aatccaacgg cgaaggcggt gtggatattg agcatctgga atcgctgatg
     8161 acggaggatg tggcggccct gatgctgacc aatcccaaca cgctgggact tttcgaacgc
     8221 aatatcgaga aggtggcggc cattgttcac ggcaaaggcg gcctgcttta cggcgatggc
     8281 gccaatgcca acgctttttt aggtaaagtt agacccggcg atctgggctt cgacgtgatt
     8341 caactgaacc tgcacaaaac cttttccaca ccgcacggcg gaggcggtcc gggcagcggg
     8401 ccggtaggcg taagcaatgc gctgcttgat tttctcccgg tcccccgcat tattcggaaa
     8461 gatgataaat tcaaatggtc ggaaaaattt ccggacagta tcggccgtgt ccgcgcattt
     8521 tatggaaatt tcaatgtcat tgttaaagcc tacacgtata tccggtcact gggagctgac
     8581 ggtttgaaac atgccacgga aatggccgtg ctcaatgcca actacatcaa ggagcgatta
     8641 aaaccgtatt ttgatttgcc ttatgaccgg gtctgcatgc atgaatgcgt cttttccggc
     8701 aacagacagg ttgctcaaag ctcggtgcat accagcgaca tcgcgaagaa gttgattgat
     8761 tacggttatc atccgccgac catttacttc ccgctgattg tgccggaagc gataatgatc
     8821 gaaccgaccg aaacggaaag aaaagagacg ctggatacgt tctgcgatgc catgattgcc
     8881 attgcccgcg aggccaagga aaatcccgaa ggcgtcaaga acgcacccgt atcaaccccg
     8941 gtgagtcggc ttgacgaagt cttggccgcg cgtaaaccgg atgtctgctg gaaaaaatcg
     9001 taagcgccat tgcgaggcga ctctcaggag ccggggtaat ctttctatgt gcgataacgc
     9061 gtcatgcaga ataatctatg aatccattaa atgagaaatc gtcaatcatc cgcaaacctg
     9121 actggttaaa agtacgacct ccgttttcca atgaatgccg gaagatacga tcagttttga
     9181 acgatctgca gttgcatacc gtctgtcagg aagcggcgtg tcccaatatg gcggagtgtt
     9241 ttgcaagcgg cacggcgact tttctgatca tgggcaatat ctgcacacgc aactgtcttt
     9301 actgtaatgt tgagcacggc aaacccgcag cgattgacga aaatgaaatt aatcatctaa
     9361 tagatgccgt gcagaaaatg aaactggagt atgtggtgat cacgtccgtt acccgcgatg
     9421 atttgcgcga tggcggtgcg cacgtatttg ctgattgtat caggcggctg cgcaaaactg
     9481 ttgaacattg caaaatagaa gtattgattc ccgattttca ggggaatccg tcggcactta
     9541 caaaggtgat tgacgtccag cccgatgtta tcaaccacaa tatggaagtt gtgaaaccga
     9601 tgtttacgcg attgcggccg cagggtaatt atgatgtttc gctgaagctt cttaaaaata
     9661 tcggtccgtc ttccgttgta tccaaaagcg gttttatgat tggcttcggc gagaaccgtg
     9721 atgatatttt gcgattgatt gatgatctgg cagcggtttc ctgcaaacgc ctgacgatcg
     9781 ggcaatacca gcagccgacg ctgaaacact ggccggtggc caaatattat catccggacg
     9841 aatttgccga gttcaaagaa atcgcttatc aaaaaggttt tcagcatgta gagtccggac
     9901 cgctggtgcg cagctcctat cacgcggcaa aagcagggtg aatggtgaat ctgatttgtc
     9961 tttaataaaa atgtctttat aaattgcgag gtacaaacgt gtccgaaaaa tatgatttga
    10021 ttgttatcgg cggaggaccg ggcggattga ctgctgcaga aatggcggcg aaaaagaaaa
    10081 aaagagtcct gatcatcgaa aaagaaggtt ggggcgggac ctgcacacac cggggctgca
    10141 ttccgaccaa agctctgctt tcctgtagca aatattacgc ggatctgaaa aaactcaaaa
    10201 ggatggggat caacatcgcg gattcatccg tcgattttcc cgccgtcaaa aaacaccaac
    10261 agcagattgt gaaggtgtcc gctctgggcg cgcaaaaaat gctcaacgat gccggtgcgg
    10321 aaatcaaaca aggtgttgga gaaattattt ctccttcgga agttcgtttt acggattcat
    10381 cagggaaaag cgaaaattta tcaactcaaa atatcttgat ggcctggggc tccattccgc
    10441 aggtgctgtc gggaattaat ctttccgaaa ggattttaac atcggacggc gttttgaatt
    10501 taaatgcgct tccttcgagc atcatcattg tcggcggcag tgtcatcggt gcggagtttg
    10561 ccaccttctt cgccgagttg gggacaaaag tgaccatggt ggaaatcctc gatacgatca
    10621 ttccactgga ggatcaggaa tgcgccgatc tgctcaaaca ggaattaacc agaatgggtg
    10681 ttgttattca tacatcgaca aaactggaga gcttgaagga tacgggaagt tctgtggcaa
    10741 tggtcgcgca aaaagacggc aagcacctgg agatcagcgc cgactatgcg ctcctttgca
    10801 caggcagaaa accgttatta tttacggcag aattggacaa atgcggaatt gaatatacac
    10861 aagccggaat caaggtggat aaaaagatga tgacgagtgt tgccggcatt tacgcagtgg
    10921 gcgatgttac cggcgggatg atgctggcgc ac
//
LOCUS       DAOSME010000068        10935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6857_length_10935_cov_20.707537, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000068 DAOSME010000000
VERSION     DAOSME010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6857_length_10935_cov_20.707537"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..>1197)
                     /gene="tgt"
                     /locus_tag="PLG98_09265"
     CDS             complement(136..>1197)
                     /gene="tgt"
                     /locus_tag="PLG98_09265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOE33489.1"
                     /translation="TARGIVQTPVFMPVGTQGTVKSMLPEEIKNCGAQMLLSNTYHLY
                     LRPGHETIKNLGGLHRFMNWPYPILTDSGGFQVYSLGTLRKITPEGVMFRSHIDGSRH
                     FLTPQKAIEIQEALGSDIMMCLDECTPYPSTPSQTEKSLNLTAKWALSCREAKTDNGQ
                     ALFGIIQGGSYPDLRRQSLEKTVLLNFDGYALGGVSVGEPKEIMYTITDVMAPLLPEN
                     KPRYLMGVGTPEDIVFGVSCGIDMFDCVIPTRCARHGLLFTNSENVVIKNARWREDAD
                     PIDETCDCYTCRNYSRAYLRHLYFAGEILAMVLNTIHNVRYYMRLMENIRLAIQENRF
                     EAFKNAFLKDRTESKKHLT"
     assembly_gap    1198..1297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1298..2106)
                     /gene="queA"
                     /locus_tag="PLG98_09270"
     CDS             complement(<1298..2106)
                     /gene="queA"
                     /locus_tag="PLG98_09270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOE33490.1"
                     /translation="MQLKDFSYDLPEELIAQHPCARRDQSRLLLMGRHKDTLADMSFF
                     QLPDLLKKGDVLVVNDSRVIPARLFGRKQTGGQLEILLLNVKKKEPTSQIWEVLIRPA
                     KRLHEGDDIRLAGHCEAKVLARISEKKWLLEFSAPDGFESYVNTFGRAPLPPYIKRMK
                     NTRPDAADLDRYQTIYARHPGSIAAPTAGLHFSDGVIQQLKDRGIQIAHVTLHVGYGT
                     FLPITTDEVEKHVMEPEHYEISPESARIINNAERVIAVGTTSTRTLESVSN"
     gene            complement(2175..2360)
                     /locus_tag="PLG98_09275"
     CDS             complement(2175..2360)
                     /locus_tag="PLG98_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOE33491.1"
                     /translation="MKDLLCVLGLVFIIEGLPYFVFPEKLKVYLAKIMTLPDSTLRFI
                     GISSMILGLILLYFGRR"
     gene            complement(2363..3139)
                     /gene="mazG"
                     /locus_tag="PLG98_09280"
     CDS             complement(2363..3139)
                     /gene="mazG"
                     /locus_tag="PLG98_09280"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOE33492.1"
                     /translation="MESLNRLIDLIRKLRAPDGCPWDRQQKKEDIGKYMLEEAYEAID
                     ALEKKNPEALKEELGDVLFQILFICEICAESGLFSLEDVMDGVNEKMIRRHPHVFGTV
                     KVTSVQDVKDNWQQIKKKERKETGREKSLFSSIPRSLPALKRAQKITALASQSGFDWK
                     DIRSVRDKLEEEWQEFEAAVRAENRIKTEEELGDILFTMVNLSRFLRVDAETALSKTT
                     EKFLRRFAHVTEQLSSRGLKPEDATLQEMDALWDEAKKKD"
     gene            3588..3803
                     /locus_tag="PLG98_09285"
     CDS             3588..3803
                     /locus_tag="PLG98_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33493.1"
                     /translation="MKKIFAIFVAMLFVASLSLAVVGCKKAEEPAVEAPAVEAPVAPA
                     PEAAPAPAPEAAPAPAPEAAPAPAPAK"
     gene            3984..4367
                     /locus_tag="PLG98_09290"
     CDS             3984..4367
                     /locus_tag="PLG98_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOE33494.1"
                     /translation="MTEKNGDVLKGLFIGGLIGIALGILFAPKSGKETRDDILLKTDE
                     MLARVKEEYEKALEKSRLAYEDSLNRLKGVNLSVKAKAGEAEGKISELAQQGSEAMLS
                     NKNKLKKAIDAGLKAYKEELGKEKI"
     gene            4431..4844
                     /locus_tag="PLG98_09295"
     CDS             4431..4844
                     /locus_tag="PLG98_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HOE33495.1"
                     /translation="MTLEIALLILVGILIVLAVGCIPLLVQIWRISKDLEMTLQTFNQ
                     SLPLLLKNLEEITTNVNSSSSVINKKIQNFAHPSNMSGLLLGDIISNIHHFAPLALKL
                     PIFRIMRNVVSIAKGLRVFVEVLLNKEKDETLKQG"
     gene            4955..5314
                     /locus_tag="PLG98_09300"
     CDS             4955..5314
                     /locus_tag="PLG98_09300"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOE33496.1"
                     /translation="MKKAQQSSSGEDYREKSLAMNGLVCARCGREFPESKRHLLTVHH
                     KDGNHHNNPADGSNWENLCVYCHEDVHSREVLAEYLGGEAHGKEVSLVYNDAQGTVGS
                     GLLAEKLKTALKKKSDK"
     gene            complement(5317..5514)
                     /locus_tag="PLG98_09305"
     CDS             complement(5317..5514)
                     /locus_tag="PLG98_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HOE33497.1"
                     /translation="MRATYKDFDATELFCPRCKKAVAVRKRLLLILQEGEKYDYTCVH
                     CGTSIGDKLVKDSAPSGLVIN"
     gene            complement(5511..7589)
                     /locus_tag="PLG98_09310"
     CDS             complement(5511..7589)
                     /locus_tag="PLG98_09310"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOE33498.1"
                     /translation="MPANKSKNFFNLYQHGFIRAAVCIPEVKVADPVFNAKKTIELAR
                     QAAQQKAILAVFPEMGLSAYSNEDLFHQDALLQGTLQAIADVKKASARINIVMIAGAP
                     LQVDCRLFNCAVVFYRGRILGVAVKSYLPNYREFYEARQFTPADAAFSSKIKLAGQND
                     IPFGADIIFKIANIKHFSFFIEICEDLWVPVPPSSFAAMAGATVIGNLSASNITIGKS
                     EYRQQLAANQSARCVSAYLYAAAGPGESTTDLAWDGHAMIHENGNLLAESPRFSQQAQ
                     IIYSDIDLDRLAQDRMRLTSFSKNAAAHKEKILSFRKTEFDVDVPTGKILLTREYPRF
                     PYIPADPSKRDQRCYEAYNIQVQGLAKRLKASGIPNIVIGVSGGLDSTHALIVAARTM
                     DTLKLPRKNVKAYTLPGFATSRKTYNNALKLMTALGVESNEIDIKPACRQMLKDIKHP
                     YANGKQKFDITFENVQAGQRSAHLFRLANMRNALVVGTGDLSELALGWSTYGIGDHMS
                     HYNVNASVPKTLIQHLIRWVAQTGQFSQDVSDILIDVLKTEISPELIPGKNKDHLVQK
                     TEDTIGPYELQDFNNFYISRFGYLPSKVAFLAYHAWKDRNKGLWPDNIAREKKNAYSL
                     KEIKKWLHVYLYRFFKTSQYKRSCIPNGPKVGSGGSLSPRGDYRAPSDSEAEIWLQNW
                     ATIPEEDIRS"
     gene            complement(7594..8361)
                     /gene="folE2"
                     /locus_tag="PLG98_09315"
     CDS             complement(7594..8361)
                     /gene="folE2"
                     /locus_tag="PLG98_09315"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOE33499.1"
                     /translation="MIDIQSQKDSRNIDIKKVGVKNIKYPVIVLDRAKGTQLVNATIN
                     MYVNLPHHFKGTHMSRFVEALNEFKGQINIKTFRTILEKMRQKLHAHSAHMEIEFAYF
                     LEKTAPVSKAKSLMEYRCIFAGESNAETSDFLVGVIVPVTTVCPCSREISQYGAHNQR
                     SVVTTKVRFKKFFWIEDLIRIVEDSASGEVYSLLKRVDEKYVTEKAFENPKFVEDVVR
                     SVAVKLNKDDNFTWYCIEAENFESIHNHSAYALVEKG"
     gene            complement(8392..8760)
                     /gene="queD"
                     /locus_tag="PLG98_09320"
     CDS             complement(8392..8760)
                     /gene="queD"
                     /locus_tag="PLG98_09320"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOE33500.1"
                     /translation="MYEVTIIKSFSAAHLLADIGGKCETLHGHNFKVEVSVAADQLDA
                     NGLLIDFRFLKKILGEILDDIDHKHLNALAHFKGINPSAENIAKYIYDQMDEKVKTAG
                     VHMVRVKIWESENAAVSYTR"
     gene            complement(8902..10293)
                     /locus_tag="PLG98_09325"
     CDS             complement(8902..10293)
                     /locus_tag="PLG98_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33501.1"
                     /translation="MKILLVRPPSFKMPIIIPNLGLGYLASVLRHNGHTVSLLDCAKT
                     KTTHRQYRDCLMDEQPDIVGIQMYTCDFSSARQCIEIAKTVNGKTVTIVGGPHPSGDP
                     EGTMEIPGLDYAFVGEAESGLPRLLEYTRSAGQVAIDSIDGLVHRQNGKIIKNKKGAI
                     QNPDDIPFPGWDLIDPQTYPAAPHGTFSKSLPVAPVITSRGCPFQCTYCGANVNTGRH
                     FRKRSVENIVEEIKYLQKTFGIREFHIEDDNFTLDKARVMAFCHKLKEENIKLHWACT
                     NGVRLDTLDKELLQNMESAGCYSLAAGIESGSPRIINDMKRNETLDIMIEKINLIARE
                     TKIRITGFFMIGYPEETRNDIEKTIELAMKLPLHRAQFSNFLPLPGTEIYDRLIQRKE
                     IAPDTLPWDLYQNNRVVYTPKNISPREMRRLMRKAFAAFYFRPRILWGLLKEIHSPGQ
                     FRTVVRRFFDVFR"
     gene            complement(10384..>10935)
                     /locus_tag="PLG98_09330"
     CDS             complement(10384..>10935)
                     /locus_tag="PLG98_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33502.1"
                     /translation="IFPFLFLASGFLFHQAIIKFKRIQYLLMFLIIIYVPSSLIAFPD
                     YLSYFNIAAGGSRNGSHWLMDSNLDWGQSLPALKKYMDKNNIDKIKLGYFGRVDPEIY
                     GIDYSLAEQKPTQGIYAISINFLVGRPYYLLKENTHELLYIDINYYDQYRYLEPSAVV
                     GHSIYIFDLRNKFSARPSGKLHY"
BASE COUNT         2672 a         2499 c         2674 g         2990 t
ORIGIN      
        1 taaaaaatta caaaaatgat caccatggtg atgatgaatc cccagtctcc gcctcctccg
       61 gccgccgggc cgccgggcgc tccgcccatt gcatatgcta acgatgtcaa tgtcatatcc
      121 tccttgcgat ttgctctatg ttagatgttt tttcgattcg gtcctgtcct tcagaaaagc
      181 gtttttaaat gcttcaaaac ggttttcttg tatggccagc cttatatttt ccatcagtct
      241 catataataa cggacattat gaatggtgtt caaaaccatt gccagtattt caccggcgaa
      301 ataaagatgt cttaaatatg cccgggaata attccggcag gtgtagcaat cacacgtttc
      361 atcaatcgga tcagcgtctt cccgccagcg ggcgtttttt ataacaacat tttccgaatt
      421 tgtaaacaat aatccgtgac gggcgcaccg ggtgggaata acgcaatcga acatgtcgat
      481 tccgcaagat acaccgaaaa caatgtcctc cggcgtgccg acgcccatca gataacgggg
      541 tttattctcc ggcagcaggg gagccattac atcggttatg gtatacatga tttccttggg
      601 ctcgccgacg ctgacgcctc ccagggcgta gccgtcgaag ttcagcaaaa cggttttttc
      661 cagggattgc ctgcgcagat caggatacga gcctccctga atgatcccga aaagcgcctg
      721 cccgttgtct gtcttcgcct cccggcatga cagggcccat tttgcggtca gattcagcga
      781 tttttcggtt tgggacgggg tcgacggata cggcgtgcac tcgtcgaggc acatcatgat
      841 atccgatccc agcgcttcct ggatttcgat cgctttctgc ggtgtgagaa aatgtctgga
      901 tccgtcaata tgcgaacgaa acatgacgcc ttcgggggtg atttttcgca gggttccgag
      961 actgtatacc tgaaaaccgc cgctgtcggt caggatcgga taaggccagt tcataaaacg
     1021 atgcagtccc cccaggtttt ttattgtttc atgaccggga cgtaaatata aatgatacgt
     1081 gttgctcaga agcatttgcg cgccgcagtt ttttatctct tccggcagca tggatttgac
     1141 cgttccctgc gtgccgaccg gcataaaaac cggcgtctgc actatcccgc gggcggtnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg tttgaaacgc tttccagcgt
     1321 gcgggtggat gtggtgccca ccgcaataac gcgctcggcg ttgttgatga tcctggcgct
     1381 ttcgggactg atttcgtaat gttccggttc catgacatgt ttttcaacct cgtccgttgt
     1441 aatggggaga aacgtgccgt aacccacgtg caaggtgacg tgagcaattt ggatgccccg
     1501 gtcttttaat tgctgtatga ccccgtctga aaaatgcaga ccggctgtcg gagccgcgat
     1561 ggaaccggga tgccgcgcgt aaatggtctg ataacgatcc agatcggcag catcgggccg
     1621 ggtatttttc atccttttta tatagggggg aagcggagcc ctgccgaagg tgttcacata
     1681 ggattcaaaa ccgtccggag cagaaaattc cagcagccat tttttttcgg aaatcctggc
     1741 taaaactttt gcctcgcaat gacccgccag acggatatca tccccttcat gcagcctttt
     1801 ggccggtctg atcagcactt cccatatctg ggaggtcggc tccttttttt tcacgttcaa
     1861 aagcagaatt tccagctgtc ccccggtttg ctttctgccg aacagcctgg ccgggatgac
     1921 ccgggaatcg ttcacgacga ggacgtcgcc ttttttcagc aagtccggca actgaaaaaa
     1981 ggacatatcc gccagggtat ccttgtgcct tcccatcaaa agcaggcggg attggtcgcg
     2041 ccttgcacag ggatgctgcg cgatcaattc ctccggcaga tcatatgaaa aatcttttaa
     2101 ttgcataggg ctgtcgtcat gaaaaagtgc tggcaggtaa ccagattgtg attttaatgt
     2161 caatgccatt gttttcagcg tctgccgaag tacaatagaa tcagaccgag aatcatggac
     2221 gatattccga tgaaccgcag cgtcgaatcc ggcaaggtca ttattttggc gaggtatact
     2281 tttaactttt ccgggaaaac aaaatacgga agcccttcaa taataaagac gagtcccagc
     2341 acacaaagca aatccttcat tttcaatcct tctttttcgc ttcatcccat aaggcgtcca
     2401 tttcttgcag ggttgcgtct tccggcttta agccgcggga tgatagctgt tctgtgacat
     2461 gagcgaaacg ccgcagaaat ttttctgtcg ttttggatag ggcggtttcc gcgtcaacac
     2521 gcagaaaccg gcttaaattg accatcgtga ataatatgtc gcccagttct tcctccgttt
     2581 tgatgcggtt ttctgccctt acggcagcct caaactcctg ccattcttct tccagcttat
     2641 cccggacact ccggatgtct ttccagtcga agccggattg cgatgccagg gccgtaattt
     2701 tctgcgccct tttcagagcc ggaagcgagc gcggtatgct ggagaacaga cttttctccc
     2761 ggccggtttc cttgcgctcc ttttttttga tctgctgcca gttgtctttg acatcctgca
     2821 cggaggttac tttgacagtg ccgaatacat ggggatgccg ccggatcatt ttttcgttga
     2881 cgccgtccat gacatcctca agggagaaca agcctgactc agcgcatatt tcgcaaataa
     2941 ataatatctg aaaaagcaca tcgcccagtt cttccttcag ggcctccgga tttttctttt
     3001 caagagcatc aatggcttca tatgcttctt ccagcatgta tttgccgatg tcttcttttt
     3061 tctgttgacg gtcccagggg cagccgtcgg gtgcgcggag ctttctgatg agatcaatta
     3121 atcggttaag gctttccatg ttcctcgttc ctcttgcttt ttcatgaatg cacggcttgt
     3181 ctttatgacg tcaacgtttc caagcccgtt ttatatatca tattcgatca gccggtaata
     3241 tacacaaaaa tggttttctg aattttacag aattaaagga aggaatgatt gtctccttta
     3301 agaaggagcc attgttttgc tgcggacggc ggctcgtttc aattgcatag acgaaagtta
     3361 atatgaataa caataatatc aagaagatgc atgcgtggct gctctttttc tgagttgcag
     3421 gaaaggggca tttctttgca taaaatatcg aaaagaaaag gcgcaaaaaa cggtgaaaat
     3481 tattcttgac aaggcaaata aaaagcgtat tcttcccgtt caatacagat aacaggcttt
     3541 taaaaaagcc gtttattatg aaatctttga aaggaggtta gccgttaatg aaaaaaatat
     3601 ttgcgatttt tgttgctatg ctttttgttg cttctttgtc tcttgcagtc gtaggctgca
     3661 aaaaagctga agaacctgca gtcgaagctc ctgcagtaga agcaccggtt gctccggcac
     3721 ctgaagcagc gcccgcgccc gctcctgaag cagcgccagc acctgctccc gaagcagcac
     3781 ctgcacctgc acctgcaaaa taattgcatc gcatccaaaa gcaggataga ttgatcggac
     3841 attccccgaa aggaatgtcc gatttttttt ctgaagaagt ttaattttta aaaatatgtt
     3901 atactcagta aaagcaaatt ttataatcat tatcctgtca atcgagcttt ggggtattta
     3961 cttttttata aggaaggtgc tttatgacgg aaaaaaacgg tgacgtatta aaaggtctgt
     4021 ttatcggggg cctgattgga atcgctctgg gtattttgtt tgctcccaaa agcggcaaag
     4081 aaacacgtga tgatatttta ctcaaaaccg acgaaatgct ggctcgtgtg aaagaggaat
     4141 atgagaaggc gctggaaaag agcaggcttg cttacgagga ttctctgaac cgtttgaagg
     4201 gtgtcaactt atccgtgaaa gcgaaggcgg gggaggcgga agggaaaatc agcgaactgg
     4261 ctcagcaggg gtctgaggcc atgctgagca ataaaaacaa gctcaaaaaa gcgatcgatg
     4321 cgggcctgaa agcgtataaa gaagaactcg gtaaagaaaa aatctgaccc tgtctggttt
     4381 ataaacatca taacaggatt cgaaaaaacg atctttaaag gagaaaatca atgactctgg
     4441 aaatcgcctt gttaattttg gttggcattc ttatcgtttt ggctgtcggt tgtattccgc
     4501 ttcttgtaca aatctggcgc atatcgaaag atctggagat gacactgcaa acgttcaacc
     4561 aaagtctgcc gctgttattg aaaaatctgg aagaaattac cacgaacgtc aacagctcct
     4621 cctcggtcat caacaaaaag attcaaaact ttgcccatcc ctcgaatatg tcaggtctgc
     4681 ttctggggga cattatcagt aatatccatc attttgcgcc gctggcgctg aaattgccga
     4741 ttttccgtat tatgagaaat gttgtgtcca ttgccaaagg tctgcgtgtt tttgtggaag
     4801 tccttttgaa taaagagaaa gatgagacat tgaagcaagg ttaacgatgg gcaaggtaac
     4861 gttttccagt aagtggggca gtgtaaaggt tcagaaaaag ccggctgcgc cttcgtccga
     4921 atcggctaaa ccgatggaag cgctggtccg ggaaatgaag aaggctcaac aatcttcatc
     4981 gggagaagat taccgggaaa agtccctggc gatgaacggc cttgtttgcg cgcggtgcgg
     5041 ccgggagttt cccgaatcaa aaagacatct tttaaccgtc catcataaag acggtaatca
     5101 tcacaacaat cccgccgatg gaagcaactg ggaaaatctt tgtgtctatt gccacgaaga
     5161 tgttcacagc cgggaggttc tcgcggagta tctgggtgga gaggcacacg gcaaggaagt
     5221 gagcctggtc tataatgatg ctcagggaac tgttggatcc ggattgctgg cggaaaaact
     5281 caaaaccgcc ctgaaaaaaa aatcggataa ataatttcag ttgatgacca gaccggaagg
     5341 ggcagaatct ttgaccagtt tgtcgccgat ggatgttccg caatgcacgc aggtataatc
     5401 gtacttttcg ccttcctgaa gaatcagcag caatcttttt ctgacggcta ccgctttctt
     5461 gcagcgggga caaaacaatt cggtggcgtc aaaatcttta taggttgctc tcatgatcga
     5521 atgtcctcct ccggtatggt tgcccagttt tgcagccaga tttcggcctc gctgtcgctt
     5581 ggcgcccggt agtcgcctcg cggcgaaagc gagccgccgg atccgacctt ggggccgttg
     5641 gggatgcagg agcgtttgta ctgcgatgtt ttaaagaatc gatacaaata aacgtgaagc
     5701 cattttttga tttctttcaa cgaataggcg tttttctttt cccgggcgat attatcgggc
     5761 cagagacctt tgtttctgtc cttccaggcg tgatacgcca gaaaagccac cttggaaggc
     5821 agataaccga atcgggaaat atagaaatta ttaaagtcct ggagttcata cgggccgatg
     5881 gtgtcttccg ttttctggac gagatgatct ttgtttttcc ccggaatcag ttcgggggat
     5941 atttccgttt tcagcacatc gattaaaata tcggaaacat cctgtgaaaa ttgcccggtc
     6001 tgggccaccc agcggatcag atgctgaatc agtgttttcg gaacgctggc gtttacgttg
     6061 taatgagaca tgtgatcgcc gatcccatac gtgctccagc ccagcgccag ttcgcttaaa
     6121 tcgcccgtgc ccaccaccag tgcgttgcgc atgttggcca gacggaaaag atgcgctgaa
     6181 cgctgtccgg cctgaacatt ttcaaaggtg atatcgaatt tttgcttgcc gttggcatac
     6241 ggatgtttga tgtccttgag catttgacgg caggcaggtt tgatatcgat ctcattggac
     6301 tcgacgccga gggccgtcat gagtttcagg gcattgttgt aggtttttcg cgatgtggcg
     6361 aatcccggca aggtatacgc cttgacgttt ttccggggca atttcagggt atccatggtc
     6421 cttgcggcga caatgagggc gtgcgtggaa tccagaccgc cggaaacacc gatgacgata
     6481 ttgggaatgc cggacgcttt cagccgtttg gccaacccct gtacctgtat attataggct
     6541 tcgtagcatc gctgatcccg ctttgaagga tcggccggaa tatacggaaa gcggggatat
     6601 tcgcgggtca gcagaatttt acctgtcggc acatcgacat cgaattcggt cttacggaag
     6661 gataaaattt tctctttatg cgcagcggcg tttttactga aggacgtcag gcgcattctg
     6721 tcctgagcga gacggtcaag atcgatgtcg ctgtagatga tctgcgcttg ttgtgaaaaa
     6781 cgcggtgact cggcgagcag gttgccgttt tcgtgaatca tggcgtggcc gtcccaggcc
     6841 aggtccgttg tcgattcgcc gggacctgcc gcggcgtaaa gataagcgga aacgcaacgt
     6901 gccgactgat tcgccgccag ttgttgacgg tattcggatt tgccgatcgt gatgttggag
     6961 gccgatagat ttccgataac ggtcgctccg gccatagccg caaaactcga gggtggaacc
     7021 ggaacccaca agtcttcgca gatttcgata aaaaagctga aatgtttaat gttggcgatt
     7081 ttaaaaatga tatcggcgcc aaaagggata tcgttctgcc cggccagttt aatttttgaa
     7141 gagaaagcgg cgtccgccgg tgtgaactgg cgggcttcat aaaattcgcg ataatttggc
     7201 agataggatt tcaccgcaac gcccagaatc cgtccgcggt agaaaacgac ggcgcagttg
     7261 aacaaccggc aatcgacctg cagcggcgcg ccggcgatca taacgatgtt aattcttgcc
     7321 gaggcctttt tgacatctgc aatggcttgc agcgttccct gcagaagcgc atcctggtga
     7381 aacagatctt cgttggaata ggccgacaaa cccatttcgg gaaagacagc cagaatggct
     7441 ttctgttgcg ccgcctggcg ggccagttcg atggttttct tcgcgttgaa aacgggatcg
     7501 gctactttga cttccggtat gcagaccgcc gcgcgaataa agccatgctg atacaggttg
     7561 aaaaaatttt tcgatttgtt cgccggcatg ccattacccc ttttctacga gagcgtacgc
     7621 gctgtgatta tgaatgcttt caaaattttc cgcttcaata caataccagg tgaagttgtc
     7681 atccttgttc aatttcacgg cgacactgcg gacaacatct tccacgaatt tcggattttc
     7741 aaaggccttt tccgtaacgt atttttcgtc aacgcgtttc agaagagaat acacttcgcc
     7801 gctcgcggaa tcttccacta ttctgattaa atcttcaatc cagaaaaatt ttttgaagcg
     7861 gacttttgtc gtcacgacac ttctttgatt gtgggctccg tattggctga tttcgcggga
     7921 gcaggggcag acggttgtga ccgggacaat cactcccacc agaaaatccg atgtttcggc
     7981 gttgctttct ccggcaaaga tacaacggta ttccatcagg ctctttgcct tggaaacggg
     8041 cgccgttttt tccaggaaat aagcaaattc aatttccatg tgggcggaat gggcatgcag
     8101 cttctgccgc attttctcca gaatagtccg gaacgtttta atattgatct ggcctttgaa
     8161 ttcattgagc gcttccacaa agcggctcat gtgcgttccc ttgaagtggt gcggcaggtt
     8221 cacatacatg ttgatcgttg cgttgaccaa ctgcgttccc ttggcccggt caaggacaat
     8281 caccgggtat ttgatatttt ttacgccgac ctttttaata tcaatattgc gagaatcttt
     8341 ctggctttga atatcaatca tggcactatg ctcttttcat cattggcgtt cttaccgggt
     8401 gtacgacacg gcggcgtttt ccgattccca gattttgacc cgaaccatgt gcacaccggc
     8461 cgttttgact ttttcatcca tctgatcgta aatatatttt gctatgtttt ccgccgaggg
     8521 attgatgcct ttaaaatggg ccagcgcgtt gaggtgtttg tggtcgatat cgtcaagaat
     8581 ttcccccagg atttttttta aaaaccggaa atcgatcaaa agaccgttgg cgtcgagttg
     8641 atcggctgcc acggatactt caaccttgaa attgtgaccg tgcaatgttt cgcatttgcc
     8701 gccgatgtcg gccagcagat gtgcggcgga aaatgatttt attatcgtaa cttcatacat
     8761 tggtcatgtg ctccgtttct taaagtagaa ttattatcat attttcaaga gatgttgaat
     8821 cattaattat tgacaaccga taatatgaag catcgttgcg tcctctgaaa tgaagggtgc
     8881 agatttcatc agggggaata tctatctgaa aacatcaaaa aaacggcgaa cgactgtacg
     8941 aaactggccc ggggaatgga tctccttgag taatccccaa agaatacggg gacgaaaata
     9001 gaacgcggcg aaggcttttc tcatcagtcg tcgcatttcc cggggcgaaa tatttttcgg
     9061 cgtatagacg acgcgattat tctggtagag atcccagggc agggtatcgg gagcaatttc
     9121 tttcctttgg atgagccgat catatatttc tgttcccgga aggggaagaa aattgctgaa
     9181 ttgggctctg tgcaaaggga gtttcatagc aagttcgatg gttttctcaa tatcatttct
     9241 cgtttcctca gggtagccga tcataaaaaa acccgttatt ctgatctttg tttcccgggc
     9301 aataagattg attttttcaa tcatgatgtc gagtgtttca tttctcttca tgtcgttgat
     9361 gattctggga gaaccggatt cgattcctgc cgctaaagaa taacacccgg cgctttccat
     9421 gttctgcagc aattctttgt ccagggtatc cagtctgacg ccgttggtgc aggcccagtg
     9481 cagcttaata ttttcttcct tgagtttatg gcagaatgcc atcacacgcg ctttgtccag
     9541 cgtgaaatta tcatcctcga tgtgaaactc cctgatgccg aatgtctttt gcagatactt
     9601 tatttcttca acaatgtttt cgacacttct tttccggaag tgtctgcctg tgtttacgtt
     9661 cgcaccgcaa taggtgcatt gaaaaggaca ccctctgctg gttatgacgg gcgctacagg
     9721 cagacttttt gaaaacgtgc cgtgcggcgc cgccggatag gtttgcgggt cgatcaaatc
     9781 ccaaccggga aaggggatgt catccgggtt ttggatggcc ccttttttgt tttttatgat
     9841 ttttccgttt tggcgatgaa ccaatccgtc gatggaatcg attgcaacct gaccggcaga
     9901 ccgggtatat tccagcaatc ggggaaggcc cgattcggct tctcccacga aagcataatc
     9961 cagaccggga atctccatgg ttccttccgg atctcccgac ggatgcggac cgccgacgat
    10021 ggtaacggtt ttgccgttta cagtcttcgc aatttcaata cattgtctgg ccgaggagaa
    10081 atcgcaggtg tacatctgga tgccgacgat gtccggttgt tcatccatga gacaatcccg
    10141 atattgtcgg tgggttgtct ttgtcttcgc gcaatcgaga agactgaccg tgtgcccgtt
    10201 atgcctgaga accgaagcga ggtatcccag tccgagattg ggaatgatga tcggcatttt
    10261 aaaactcggc ggacgtacaa gcagaatttt catatcattg aactctgtaa tggattcatt
    10321 gcttcatgct catcgtgatg ttgacggtgt tcatatccgt tgccagtaaa taatgaataa
    10381 ccatcaataa tgtaactttc ctgaaggccg tgcggaaaat ttatttctca gatcgaaaat
    10441 gtaaatcgaa tgaccgacaa cggcagacgg ttcgagatac ctgtattgat catagtagtt
    10501 gatgtcgatg tacagcaatt cgtgcgtgtt ttcctttaaa agataataag gtcttcccac
    10561 gaggaaattt atgctgatgg cgtatattcc ttgcgttggt ttttgttccg caagagaata
    10621 gtcgatgccg tatatttcag gatcaacccg tccaaaatac cccagtttga ttttatcaat
    10681 gttatttttg tccatgtatt tttttaaggc aggcaagctc tgtccccagt ccaggttgga
    10741 atccatcagc cagtggcttc cgtttcggga gcccccggcg gcgatattaa aataagataa
    10801 ataatccgga aaagcaatga gcgaggatgg aacatatata atgatgagaa acatcagaag
    10861 atattgtatg cgtttaaatt ttatgatggc ctgatgaaaa agaaagccgg atgccagaaa
    10921 cagaaagggg aatat
//
LOCUS       DAOSME010000069        10756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_6996_length_10756_cov_23.746753, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000069 DAOSME010000000
VERSION     DAOSME010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6996_length_10756_cov_23.746753"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLG98_09335"
     CDS             <1..570
                     /locus_tag="PLG98_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE33503.1"
                     /translation="TEASGALCIKVPWPGIMRGVYGDPKRFQETYFEQFPGYYVTGDG
                     CRRDKDGYYQITGRIDDVINVSGHRMGTAEVESALVAHKAVAEAAVVGMPHDIKGQGI
                     YAYVTLKTGVEKSDALKKELIAHVRTVIGPIATPDKIQWADGLPKTRSGKIMRRILKK
                     VAANDLGNLGDTSTLADPSVVDDIVKNRL"
     gene            689..1459
                     /locus_tag="PLG98_09340"
     CDS             689..1459
                     /locus_tag="PLG98_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOE33504.1"
                     /translation="MNIIACVKQVPDTEAQIKVKADGSGIDDSQIKWVMNPYDEFGVE
                     EALKLKEKNGGDVTIVSVGPARAMETIRTALAMGADKGIHISDPALDNADAFVTASAL
                     AAAIKGLPHDIIFCGQRAIDDDSGQVGAMLAELLGIPQLTLVTKLDYAGSSVKVIRPI
                     EGAQLSIETSLPCVVTAQKGLNEPRYASLPGIMKAKKKPVDVKNAAALGIATDVKANV
                     AKMVPPPARAAGKIICGDDAAQKAAELARCLREEAKVI"
     gene            1483..2454
                     /locus_tag="PLG98_09345"
     CDS             1483..2454
                     /locus_tag="PLG98_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOE33505.1"
                     /translation="MAKGIYVIAEQRDGALRKVSFELVSAARKLADQTSDEVCAILLG
                     SGVESMAAELGKFGADKVFVGDNAALEPYITEAHAQVVAKILKDNDAAIALFGASVQG
                     KDLSARVAAKLAGGLATDCTDVKIDGGKLIAVRPMYAGKCFGEVAFNTTPAMASLRPN
                     VFPAVENAKAGAVTKVDVAVDAQKSKILEVQKDTSGKVDLTEANIIVSGGRGMKGPDE
                     YKILEELADVLKGTVGASRAAVDAGWRPQKDQVGQTGKVVSPNLYIACGISGAIQHLA
                     GMSSSKCIAAINKDAEAPIFAKADFGVVDDLFKVVPELTAVCKKMLA"
     gene            2612..4777
                     /locus_tag="PLG98_09350"
     CDS             2612..4777
                     /locus_tag="PLG98_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOE33506.1"
                     /translation="MEHHGISPIGIGNEGRQVFWNAHSFEPFLFLFTAIAMAIFAYGV
                     YKRWKLWKAMGKDEIRVDELPARIKSLIMNGFFQVKTWKDIYPGIMHGCIFFGFFVLI
                     FGAAFDAGEFHITEPLFSWAFLRGSFYLGFSFLMDLFGLLVLIGVLMAAYRRYIQKPD
                     RLTYKGTPDTTPEDGIVLLLIGGIIVTGFIIEALRIHATLDPLTGYATWEGWSFVGWT
                     LANVISGLDIEAAKTWHSISWWTHTFIALGFIAYIPYSRLMHIITTPANHFFATLKPT
                     GYVEPIRDIENQESFGVSKLDEFTWKQIFDSDACTKCGRCQDGCPAYLTGKHLSPKKL
                     VQDIKTFWLEQAPLAATKAVVPVAEGAEAAAPAEAAEGETPEKALIGDVVDLNAVWDC
                     TNCMYCMENCSSSIEHIQKIIDMRRYKVLTEADFAPELQLTYRNMENNSNPWGIGGHL
                     RGDWAKELGIKTLAEDPNVEYLFYVGCSGSFDDRGKKISVAFAKILQAAGVSFGILGT
                     EESCCGDSAMRGGNDYLFQMQAQANIEVMNGYGVKKIIAICPHGYNCIKKDYPNFGGN
                     YEVYHHTEIIADLIAKGKIKFTQPLNGTFTYHDSCFLGRYNKVYAQPRSILNAIPGTK
                     LVEMERNLSKSFCCGAGGARMWMEEHGDRINNARTDQAIAVNANTVAVGCPFCLTMIS
                     DGIKDRQKIDSMIALDIAEIVAQAMGIEEVKVNFDTCSV"
     gene            5028..>6655
                     /locus_tag="PLG98_09355"
     CDS             5028..>6655
                     /locus_tag="PLG98_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOE33507.1"
                     /translation="MAENISYASKPWLKSYEKGVPEIINYEELTMPDYLERSAKDFPN
                     DTAMVFVGYKMTYRQFKDQVDRFATCLTDFGIKKGDAVAILLPNSIPCVVAYYAILKI
                     GAIVVLNNPLYSDPELEHQFTDSDSKVLITLDALGNRMIDLRPKTKIKQIVYMGIGDF
                     LNPIKRFLGKKLKKFPFADVKAAPDVYRWMDCIKKYQPNPPKVKLTFDDIAMYQYTGG
                     TTGVSKGVILTHGNLSKQVQQINAWFPTFGRGGETMLGALPYFHVFGMSCSMNLSVAL
                     GWNQLLIPRPQPQPLLEAIREYKPTFTPLVPTMFIGMLNHPDLKKTDMSCIKGAFSGS
                     APLPVEVIQEFQKQTGSYISEGFGMTETCPVTHMNPFIGKQKIGSIGLPASDTLARIV
                     DLDDGVTDMPIGKPGELIIKGPQVMKGYKGMPEETATVLKDGWMYTGDIATMDEDGYF
                     YIVDRKKDMIISGGYNVYPRDIDEVYYGNPKVQEACSIGIPDAKRGENVKLFIVLKEG
                     QTATAEEMIEYGKAHLATYKLPTEVEFRKELPKST"
     assembly_gap    6656..6755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6756..7439
                     /locus_tag="PLG98_09360"
     CDS             <6756..7439
                     /locus_tag="PLG98_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE33508.1"
                     /translation="TELLDAATICEKDEKIKAVILTGAGDKAFVAGADIARMQNATSL
                     EMVPVMELGHRTFRLIETMPKPSIAAVNGFALGGGTELAMSCDIRFASEKAVFGQPEI
                     LIGVVPGWGGTQRLPRLVGMGIAKELILGGGQINAQRAYEIGLVNRVVPPESLMEEAR
                     KFASKLTKLPPFAMKMAKDAINYGYDLSLDNANKLEIACILQCFSTQDQKEGMKAFVE
                     KRKPTFTGK"
     gene            complement(7530..>9122)
                     /gene="gpmI"
                     /locus_tag="PLG98_09365"
     CDS             complement(7530..>9122)
                     /gene="gpmI"
                     /locus_tag="PLG98_09365"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOE33509.1"
                     /translation="RKGPVLLIIMDGMGIGKADESNAVFMANTPHLDTLFKSDLYTQL
                     KAHGMAVGLPSDDDMGNSEVGHNAIGAGRIFDQGARLVNKALKSGAIFQTAVWENIAK
                     RAQRGGTVHFIGLLSDGNVHSHIEQLFIMIDKCAELNFRKVRIHPLLDGRDVGERTAL
                     DYVVPTEEKLKKISGEKGFDYAIASGGGRMKVTMDRYNSDWTIVKRGWDAHVRGEGRT
                     FSSASEAVKTFYAEDSKATDQYLPPFVITDASGKPVGRIQDGDAVIFFNFRGDRAIEI
                     SRAFSEKDFSEFDRGALPDIFYAGMMEYDGDAHIPHNFLVQPPAIDRVISEYLCAEGV
                     KTFAVSETQKFGHVTYFWNGNRSGYIDETLEKYVEIPSDRIEFDKAPQMKAREITDAV
                     IDLLKSGHYRFGRLNYPNGDMVGHTGSMDAAIIAVETVDQCVGALLTAVKDIGGIAVI
                     TSDHGNADEMFTIDKNGAKSVKTAHSLNPVPFIIYDPQYRGEYRMADLKEKGLSNIAA
                     TLLNLLGFEKPEDYDPSLIEMT"
     assembly_gap    9123..9222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9223..9797)
                     /locus_tag="PLG98_09370"
     CDS             complement(<9223..9797)
                     /locus_tag="PLG98_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOE33510.1"
                     /translation="MKDVVIVSGARTAVGEFGGSLKGIKTVDIGALVMKEAMKRAGLR
                     PAIDDFHKSCRPDAFGNFEMTDINKKYYDFDQSLQPVYVDEVIMGNVLQGGQGQNVAR
                     QACIYAGFPEETNAYTINKVCASGMKAIALAAQAIAAGDADIIVAGGMEVMSNAPFVV
                     PQARWGYRMSMPFSQITDMMVYDGLYEIFNG"
     gene            10326..>10756
                     /locus_tag="PLG98_09375"
     CDS             10326..>10756
                     /locus_tag="PLG98_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOE33511.1"
                     /translation="MESIICKHWHHCPSEEVTTLLNTDADKGLSPFEAKHRLEKYGPN
                     SVTMKKQKGPLMRFLLQFHQPLIYILMAAGAVTFFLQEWVDSSVIFGVVIINAMIGFV
                     QESKAESAIESLKKMMTTQTTVLRDGKWQEIDAVELVIGDIV"
BASE COUNT         2678 a         2612 c         2697 g         2569 t
ORIGIN      
        1 accgaagcca gcggcgcgct gtgcatcaaa gttccctggc ccggcatcat gcggggcgtt
       61 tacggcgatc ccaagcgctt ccaggaaacg tatttcgaac agttccccgg ctactatgtg
      121 accggcgacg gttgccgccg tgacaaagac ggttactatc agattaccgg ccgtatcgac
      181 gacgttatca acgtgtccgg tcaccgcatg ggcaccgccg aagtggaaag cgcactggtc
      241 gctcacaaag ctgttgctga agctgccgtt gtcggcatgc cgcacgacat caaaggccag
      301 ggcatttacg cttacgttac cctgaaaacc ggcgtggaaa aatccgacgc gctgaagaaa
      361 gaactcatcg ctcatgtccg cacggttatc ggacccatcg cgacccccga caagattcag
      421 tgggctgatg gtcttcccaa gacccgttca ggcaagatca tgagaagaat tttgaagaaa
      481 gttgccgcca atgatctggg caacctcggc gacaccagca cgctggcgga tccgtccgtg
      541 gttgacgata ttgtcaaaaa cagactgtaa aacattaagt ccggttcccc ggaaatggga
      601 accggacttt ttaatgaaat aaagagaaaa ataaaattgt gcgttttcct ttaaaggaaa
      661 aatgcgcaga agaaggaggt tggacaaagt gaatattatc gcatgcgtaa aacaagtacc
      721 ggataccgaa gcccagatca aagtcaaagc tgacggatca gggatagatg attcccagat
      781 taaatgggtc atgaatcctt acgatgaatt cggtgtcgaa gaagctctga aactgaaaga
      841 gaaaaacggc ggggacgtaa ccatcgtttc cgtgggaccg gcaagagcca tggaaaccat
      901 cagaaccgct ctggccatgg gcgctgataa aggcattcat atcagtgatc ccgctctgga
      961 caacgcagat gcttttgtaa cagcatctgc tctggctgcc gccatcaaag gcctgcccca
     1021 tgacattatt ttctgcggac agcgcgccat tgatgatgac tccggtcagg tcggcgccat
     1081 gctggccgag cttctgggca ttccccagtt gaccctggtc accaaactgg attacgccgg
     1141 atcttccgtg aaagtaattc gtcccattga aggtgcacag ctctccattg aaaccagttt
     1201 gccctgtgtc gtcacggcgc agaaaggtct caatgaaccc cgctacgcgt cacttcccgg
     1261 cattatgaaa gccaagaaga aacccgtgga tgttaaaaac gcggccgctt tgggtattgc
     1321 taccgatgtt aaggccaacg tagccaaaat ggttccgccc cccgcgcgag cagccggcaa
     1381 aatcatttgt ggtgatgacg cggctcagaa ggcggcggaa ttggcccggt gtctcaggga
     1441 agaagctaaa gttatttaat ttaaaagcgg aggaattgaa aaatggcaaa aggaatatat
     1501 gtaattgcag aacagagaga cggcgccctg cgtaaagtaa gctttgagct tgtttccgcg
     1561 gccagaaaac tggctgatca gacaagcgat gaagtttgcg ccattctgct tggttctggt
     1621 gtagagagta tggctgccga attgggtaaa ttcggtgcag ataaagtttt tgtcggcgac
     1681 aatgcggctc tggagcccta tattaccgaa gcacacgctc aggttgttgc caaaatatta
     1741 aaagataatg atgcagcgat cgcattgttt ggcgcttccg ttcagggcaa ggatctttcc
     1801 gcccgcgtag ctgccaagct ggccggcggt ctggcgacag actgcaccga cgtcaagatt
     1861 gacggcggga aactcatcgc cgttcgtccg atgtacgccg gtaaatgctt cggcgaagtg
     1921 gccttcaata ccactccggc gatggcatcg cttcgtccca atgttttccc ggcggtggaa
     1981 aacgccaaag ccggcgctgt caccaaggtt gacgttgctg ttgacgctca gaagagcaag
     2041 attctggaag tgcagaaaga caccagcggc aaggttgact tgactgaagc caacattatt
     2101 gtttccggcg gtcgtggtat gaaaggtccg gatgaataca agattctgga agaactggct
     2161 gacgtgctca aaggcaccgt cggtgcttcc cgtgcggcgg tggatgccgg atggcgtccc
     2221 cagaaagatc aggtcggaca gaccggtaag gttgtctctc cgaatctgta catcgcctgc
     2281 ggtatttccg gtgcgattca gcatctggcg ggcatgagct catcgaaatg catcgcggct
     2341 attaataagg atgcggaagc tcccattttt gcaaaggccg atttcggcgt tgtggatgac
     2401 ttgttcaaag ttgttcccga actgaccgcc gtctgcaaga aaatgctggc ttaagatatt
     2461 cacaaagctt ccggtaagga gaacccttgc cggaagctta caataaaaca gccgcaaaga
     2521 atttttatta aattatcaat tcaggtcttc ggacttgaaa gaaaaattaa gttaaaaata
     2581 cataaaaaac ctcaattatt tcggaggatc aatggaacat catggtatta gccctatcgg
     2641 aatagggaat gaaggtcgtc aggttttctg gaacgctcat agttttgagc cgttcctttt
     2701 tctttttact gccattgcca tggcgatatt cgcctacggt gtttacaaac gctggaagct
     2761 ctggaaagcg atgggcaagg atgaaatccg tgtggacgag cttcctgcac gaataaagtc
     2821 tctcattatg aacggatttt tccaggtcaa aacatggaaa gatatatacc cgggaattat
     2881 gcatggctgt atcttttttg gtttctttgt tttgattttc ggagccgctt ttgatgccgg
     2941 agaatttcac atcacggagc cgttgttcag ctgggcgttt ttgcgcggca gtttttatct
     3001 cgggttctcc ttcctgatgg atctgttcgg tctgctggtg ctgatcggag tattgatggc
     3061 cgcctatcgc cggtacattc aaaaaccgga tcgcctcacc tataaaggca ctccggatac
     3121 cacgcctgaa gatggcatcg ttcttttact gatcggcggc atcatcgtga ccggattcat
     3181 tattgaagcc ttgcgtattc acgctacttt ggatccgctt accggttatg cgacgtggga
     3241 agggtggtca tttgtcggct ggaccctggc caatgtgatt tccggcttgg atattgaagc
     3301 agccaaaacc tggcattcca tcagttggtg gacgcacaca tttattgcac tcggttttat
     3361 tgcttacatt ccatactccc gtttgatgca tatcattacc acaccggcca accatttctt
     3421 cgcgacgctg aagccgaccg gatatgtgga accgattcgt gatattgaga atcaggaatc
     3481 cttcggtgtg tccaagctgg atgaattcac ctggaagcag atttttgatt ccgacgcctg
     3541 cacgaagtgc ggacgctgcc aggatggttg cccggcctat ctgaccggca aacatttgtc
     3601 gcccaagaaa ctggttcagg atatcaagac attctggctg gaacaggcgc ctctggcggc
     3661 caccaaagcc gtggttcccg ttgcggaagg tgctgaagcg gcagctcccg ctgaagcggc
     3721 cgaaggcgaa accccggaaa aagctctgat cggcgatgtg gtcgatctga atgccgtatg
     3781 ggattgcacc aactgtatgt attgtatgga aaactgctca tcctcgattg agcatatcca
     3841 gaagattatt gacatgcgtc gttacaaagt tctgaccgaa gccgatttcg ctcccgaact
     3901 gcagttgact tacagaaaca tggaaaacaa ctccaacccg tggggtatcg gtgggcatct
     3961 gcgcggtgac tgggcgaaag aactgggtat caagacgctg gcggaagatc ccaatgttga
     4021 atatctcttc tatgtcggct gttccggctc ttttgatgat cgcggcaaga agatttctgt
     4081 ggcgttcgcc aaaatcctgc aggcggccgg tgtcagcttc ggtattctgg gaacggaaga
     4141 aagctgctgc ggtgattccg caatgcgcgg cggtaacgat tacctgttcc agatgcaggc
     4201 tcaggccaat attgaagtca tgaacggcta tggcgtgaag aagatcatcg ccatttgtcc
     4261 gcacggttac aactgcatca agaaagatta tcccaatttc ggcggcaact acgaagtgta
     4321 tcatcacacg gagattattg ccgatctgat tgcgaagggt aagattaaat tcacacagcc
     4381 gttgaacgga accttcacct atcatgattc ctgtttcctc ggccgttaca acaaggttta
     4441 cgctcaaccg cgcagcattc tgaatgctat accgggaacg aagcttgtcg aaatggaacg
     4501 caacctgtcc aagagcttct gctgcggcgc cggcggagcg agaatgtgga tggaagaaca
     4561 tggcgaccgc atcaacaacg cgaggaccga tcaggcgatt gcggtcaacg caaacacggt
     4621 ggctgtcggt tgtccgttct gcttaaccat gatttcggac ggtatcaaag acagacagaa
     4681 aatcgattcc atgattgcat tggatattgc tgaaattgtt gcccaggcaa tgggtatcga
     4741 agaagtgaag gtcaattttg acacctgctc tgtgtaagta aacattcatc gatgttttgt
     4801 aaaagcccgg cttgatgtcc agccgggctt tttttataag gatgtttgca acgtcctgtc
     4861 attttgccgc tttgtttttg aaattcaaac gcggctcctt tttcccggcg ctccgggctc
     4921 cactttgcct gttcttgcgg gccggattta aaaatatatt tgaatttata cttcagaaga
     4981 gtataatgcg ccattccaaa aaattttatt aaggggaggg gagtattatg gcagaaaaca
     5041 tctcttacgc gagcaagccc tggctgaaat cctacgaaaa gggcgttcct gaaatcatca
     5101 attatgaaga actgacgatg cccgactatc tcgaaagatc cgcaaaagac tttcccaatg
     5161 atacggccat ggtatttgtc ggttataaaa tgacataccg tcaattcaaa gatcaggtag
     5221 atcgttttgc cacctgtctg acggatttcg gcattaaaaa aggagatgcc gtggcgattc
     5281 ttcttcccaa cagcatcccc tgtgtagtgg cctactacgc tattttaaag atcggtgcga
     5341 ttgttgttct gaataatccg ctctattccg atcccgaact ggaacatcag tttacagact
     5401 ccgattccaa ggttctgatt acgctcgatg ccctcgggaa ccgcatgatt gatttgcggc
     5461 cgaaaacaaa aatcaaacag attgtttata tgggcatcgg ggacttcctg aatccgatta
     5521 agcgatttct tggtaaaaaa ttgaaaaaat tccccttcgc cgatgtcaaa gcggctccgg
     5581 acgtgtatcg ctggatggat tgcattaaaa aatatcagcc gaatccgccg aaagtgaaac
     5641 tgaccttcga cgatatcgcc atgtaccaat ataccggcgg tacgaccggt gtgtccaaag
     5701 gcgttatcct gacgcatggg aatttaagca aacaggtgca gcagattaac gcctggttcc
     5761 cgacattcgg ccgcggtgga gaaaccatgc tgggcgcatt gccgtatttc catgtgttcg
     5821 gaatgtcctg ctccatgaat ctgtctgtcg ccctgggctg gaatcagctc ctgattcccc
     5881 ggccgcagcc gcagccgctt ctggaggcca ttcgggaata caaacccaca tttacaccgt
     5941 tggtaccgac catgtttatc ggcatgctca accatcccga tctgaagaaa acggacatga
     6001 gctgtatcaa aggagctttc tccggcagcg cgcccctgcc cgtggaagta attcaggaat
     6061 tccagaaaca gaccgggtct tatatttccg aagggttcgg catgacggaa acctgtccgg
     6121 taacgcacat gaacccgttt attggaaaac agaagatcgg cagcatcgga cttcctgcct
     6181 ctgatactct tgcccggatc gtcgatctgg atgacggtgt tacggatatg ccgattggaa
     6241 aacccggaga gctgatcatc aaggggccgc aggttatgaa ggggtacaag ggaatgcctg
     6301 aggaaacggc caccgttttg aaagacgggt ggatgtacac cggagacatt gccacgatgg
     6361 acgaggatgg atatttttat attgtcgacc gcaagaagga tatgattatt tccggcgggt
     6421 acaatgttta cccgcgggat atcgatgaag tctattacgg aaatccgaaa gttcaggagg
     6481 cctgctccat cggcatcccc gacgcgaagc gcggagagaa tgtgaaactc ttcatcgttc
     6541 tgaaagaagg ccagaccgcc acggcggaag aaatgatcga atacggcaag gctcatctgg
     6601 cgacctacaa actgccgacg gaagtggaat ttcgcaaaga acttcccaag agcacnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacgga acttctggat gctgcgacca
     6781 tctgcgaaaa ggatgaaaaa atcaaggcag taattctaac cggtgcggga gacaaggcgt
     6841 ttgtggccgg agcggatatc gcgcggatgc agaatgccac atccctggaa atggttcccg
     6901 tcatggaatt ggggcatcgt acattcaggc tgatcgaaac catgccgaaa ccgtctattg
     6961 ccgctgtcaa cggttttgcg ctgggcggcg gcacggaact tgccatgtcc tgcgatatcc
     7021 ggtttgcatc ggagaaagcc gttttcggcc agccggaaat tctcatcggt gttgttcccg
     7081 gatggggcgg cacacagcgt cttccccggt tggtcggcat ggggattgcc aaggaactga
     7141 ttctgggcgg cggacagatc aatgcccagc gtgcttatga aatagggctg gtgaatagag
     7201 tggttcctcc ggaaagtctt atggaagaag caagaaaatt tgcgtcgaag ctgaccaagc
     7261 tgcctccttt tgccatgaag atggccaagg acgcgatcaa ttacgggtac gacctgtcgc
     7321 tggataacgc caataaactg gaaatagcct gcatcctcca gtgtttcagc acccaggatc
     7381 aaaaagaagg catgaaagca tttgtggaaa aaagaaaacc aacgtttacg gggaagtaaa
     7441 ctcaatgttc tacgttcaaa gttcaacgtt aaaaaatgta acgtagaaca tagaacgtag
     7501 aacgtagaac gtagaacgga gaacgtttgt taagtcattt caatcagtga cggatcgtaa
     7561 tcttccggtt tctcaaatcc cagaagattc aaaagagttg ccgcgatatt ggacaaacct
     7621 ttttccttga gatccgccat ccggtattcg cctcgatact ggggatcgta aataataaaa
     7681 ggcaccggat tcagggagtg cgcggtcttg acggatttgg cgccattttt atcgatcgta
     7741 aacatctcat ccgcgttgcc gtgatccgag gtaatgacgg caatgccccc gatgtctttg
     7801 acggcggtca gaagcgcgcc cacacattga tccacggttt ccaccgcgat aatggccgca
     7861 tccatcgaac cggtatggcc gaccatgtcg ccgttcgggt aattaagtct gccgaaccgg
     7921 taatggccgc ttttcaacag gtcaatcacc gcatccgtta tttcccttgc cttcatttgc
     7981 ggggccttgt caaattcaat ccggtcggaa ggaatctcca catatttttc cagcgtttca
     8041 tcgatataac cggaacggtt gccattccag aaataagtca catgaccgaa cttctgggtt
     8101 tcggacaccg caaacgtttt tacgccttcc gcgcagagat actcgctgat gacgcgatca
     8161 atcgccggag gttgcaccag aaaattgtgc gggatatgcg catcgccgtc atactccatc
     8221 atccccgcat aaaagatatc cggcagtgct ccccggtcga attcggaaaa atccttttcg
     8281 gagaaggctc tggatatctc gatggcgcgg tcgccgcgga agttgaaaaa gatcacggcg
     8341 tcgccgtcct gaatgcgacc aaccggtttt cccgaagcat cggtaatgac aaacggcggc
     8401 aggtactgat ccgttgcctt ggaatcttcc gcgtaaaacg tcttgaccgc ctccgatgcg
     8461 gaggaaaacg tccggccttc gccccgtacg tgcgcatccc atccgcgttt cacgatcgtc
     8521 cagtcggaat tgtaccggtc catggtgacc ttcatgcgtc cgcctcccga ggcgatggcg
     8581 taatcgaagc ctttttcgcc ggagattttt ttgagttttt cctcggtggg aacaacatag
     8641 tccagcgccg tccgttctcc gacgtccctg ccgtccagta ggggatgaat ccgaaccttc
     8701 ctaaaattta attctgcgca tttgtcgatc ataataaaca gctgctcaat gtgcgagtgg
     8761 acgttgccgt cggataacag gccgatgaaa tgaaccgttc ctccgcgctg agcgcgtttt
     8821 gcgatatttt cccataccgc tgtctgaaag attgctccgc tcttcagagc tttgttgacc
     8881 agacgggcgc cctgatcgaa aatcctcccg gcaccgatgg cattgtggcc gacttcgctg
     8941 ttgcccatat cgtcatccga cggcaggccg acggccatgc cgtgggcttt caactgcgtg
     9001 tacagatccg atttaaataa tgtgtcaaga tggggggtat tggccatgaa aacggcgttc
     9061 gattcatccg ctttcccgat gcccatcccg tccatgataa tcagcaaaac gggtcctttc
     9121 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagccgtt gaaaatttca
     9241 tagagaccgt catagaccat catgtcggta atctgggaaa aaggcatgct catccggtag
     9301 ccccaacggg cttgcggtac gacaaacggc gcattgctca tgacttccat gccgccggca
     9361 acaataatgt cggcatcgcc ggccgcaatc gcctgagcgg ccagtgcaat ggctttcatg
     9421 cccgaagcgc agaccttgtt aatcgtatag gcattggttt cttccggaaa acccgcataa
     9481 atacaggcct gccgggcgac attctgtccc tgtccgccct gcagaacatt gcccataatg
     9541 acttcatcca cgtaaacagg ctgaagggat tgatcaaagt cgtaatattt tttattgatg
     9601 tccgtcatct cgaaattgcc gaaggcatcg ggacggcagc ttttatgaaa atcatcaatg
     9661 gccggtctca gcccggcgcg tttcatggct tccttcatca ccagtgcccc gatatcaacc
     9721 gtcttgatac ctttcaacga tccgccgaat tctcccacag cggttcgggc gccgctcaca
     9781 attactacat ctttcatgct taactccttt caatgttcaa aatattttat agccctgttg
     9841 agtacaaccc ggcaacgacg ggcatttctt ccatagaacc atgcgattgt tagcataatt
     9901 tccaataaaa tgcatcaagc aaggttggac agattcaaga aaaaccggat cgtttttttg
     9961 taaacgtctg aaccgtttca tcaggccgat gtcatcacct gcctggttgt caaaacttta
    10021 atttcattac ttatttcttg tatttttcgt tttggaagcg caacgatttt agcgtcggca
    10081 aaccatccgg tctgcggtgg aaaaataatt ccggatgaaa aaacttgcta atgtgtccgc
    10141 atgtctgccg gttattcaaa aaaatgtccg gtttaaattg acaaatgcac gtcgcatagg
    10201 ctacactgct gacaaatctt aaagaatctt tttgttcatc acgctcatta ccgaaccttc
    10261 aaacgacgtc accgctatac ccctcttcgc agggtgttac ggcaaaaaaa atcaggagaa
    10321 gcttcatgga atccattata tgcaagcact ggcaccactg tccctctgaa gaagttacaa
    10381 cgttgctcaa taccgatgcg gacaaaggcc tttcgccttt tgaggccaaa caccggctgg
    10441 aaaaatacgg tcccaattct gtcaccatga aaaagcagaa agggccgctg atgcggtttc
    10501 ttctccagtt ccatcaaccg ctgatttaca ttctgatggc ggccggggca gtaacctttt
    10561 tcttgcagga atgggtggat tcgtccgtca tcttcggcgt tgtgatcatc aatgccatga
    10621 tcggctttgt gcaggaatcg aaggcggaga gtgccatcga atcgctgaaa aaaatgatga
    10681 cgacgcaaac gactgtgtta cgcgacggca agtggcagga aatcgatgcg gtggaacttg
    10741 tgatcggcga tattgt
//
LOCUS       DAOSME010000070        10686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_7054_length_10686_cov_23.617063, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000070 DAOSME010000000
VERSION     DAOSME010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7054_length_10686_cov_23.617063"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLG98_09380"
     CDS             complement(<1..866)
                     /locus_tag="PLG98_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE33512.1"
                     /translation="MDFSLNESQLSYLDTVRKFVKNEITPRILDMEKEHVFPWEIINK
                     AWKTGLINLSIPESVRGYEMDLFTSALVVEGLSYGDTGISTSAMCNDLANVVIGLHGT
                     DEQKNLFLKPFVEKPILASFCLTEPNAGSDNSMMAAFISKREDGKYILNGAKCFITNA
                     SYASQFTVLCKTGKPTGNFMACVIVPVEHVTSYDIPDIGNATREISVPAGGKIIIGKP
                     EDKLGQRLSNTASVTFEDVVVFPNHIIGDRRLGFKYIIDVLDYARPMVAAIGLGLAKR
                     AFDLTVEYTRER"
     gene            complement(972..2417)
                     /locus_tag="PLG98_09385"
     CDS             complement(972..2417)
                     /locus_tag="PLG98_09385"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOE33513.1"
                     /translation="MIQEKDIKNIPLKIQDNTFRDGHQSIYATRMRTEDMIPIAEEMD
                     QCGFHAMEVWGGATFDTMHRFLGEDPWARPRILKQYIKKTPFAMLIRGQNLVGYRHYA
                     DDVVRAFVDKACEIGIDVFRCFDALNDFRNMESCAERIKANGRIFQGAVCYSLTDVRL
                     GGDVYNLDYYVRKAKALEQMGANAICIKDMGGIMSPFDAYDLVTAIKKEINLPLHLHS
                     HYTSGMASMTYLKAIEAGVDVVDTCLAPFALRTSMPAIEPIVAALRGTSRDTGIDLYK
                     LLALGEHLENVAPKYNSHLATHKLSIIDNGVLAHQIPGGMISNLTGQLKEMNALDRLP
                     EIYKEVVQTRKDMGSPPLVTPVSQIIGAQAVLNVLFGRYVRVSNQLKDLALGLYGKTP
                     MPIDAALTKKILKNYKRGQEPVSGRPADYLEPEIEAAKKAMGDLARSDEDVLAYILYP
                     ATMTKFLQSKYGIEPPRATGDDNAQKKGAAA"
     gene            complement(2460..3413)
                     /locus_tag="PLG98_09390"
     CDS             complement(2460..3413)
                     /locus_tag="PLG98_09390"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOE33514.1"
                     /translation="MKDDKNIAAVFPGQGSQRPGMGKDFYEQIPVCRQTFEEAADALG
                     WNIMDMCFGEDERLNLTEYTQPCIVTTEICMLRGLNERYGFAPDYFGGHSLGEFTALV
                     AAGVLPLFDTLKIVQIRGRLMQNAVPVGVGGMAAVISENIDMNMLTKTMEGLKAGIAN
                     INSANQVVISGELPAIDEAEKRFTENFPEGKSFRFIRLNVSAPFHSTLMKTIEGRFAE
                     TLEKFGSHLNPVNAPRVTSNFTGGFHAGDKADVKKNLVRQISHTVNWRKNMQALAENT
                     QAIYEIGPGRPLRDFFKTIGVTCQSITGLNAAEKVFKATCQ"
     gene            complement(3702..3998)
                     /locus_tag="PLG98_09395"
     CDS             complement(3702..3998)
                     /locus_tag="PLG98_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33515.1"
                     /translation="MNSLKKAIFIVCFFNLLGLWSLSLSGVAIANQNEQEASLEKCIK
                     SCEDSKQVCWNMDPDRRLCEAQFQLCMENCNKKYNAPVVEDVKPPEPKKTRTLH"
     gene            4386..5549
                     /locus_tag="PLG98_09400"
     CDS             4386..5549
                     /locus_tag="PLG98_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOE33516.1"
                     /translation="MKLDDIKKIAVIGSGAMGNGIAQVCAQAGIKAVMVDIKQEFLDK
                     GMATVNKSLDTLVGKGKMTAEKKAEVMGNLSTSLSNTEAVKDVQVIIEAVPEIMSLKK
                     TVCAEISAAAPADALIASNTSTMSISEIATAGKNPERILGMHFFNPAPVMKGVEVIYA
                     KQTSDANVDLLCELTKKIGKVPIKVLKDSPGFIVNRIGAPNQALISAILDEGKIKVDT
                     IDAVMKAKAGMPMGPFELADYVGIDVFYHTLKYYSETLSPEFKPGKTLQTLLDSGKLG
                     MKSGQGIYTWEGGKAKIDMSVESQDFGPLDFLAIQINEAVRVYKEKIAASAADIDLGM
                     VHCMRAFAGPFAFGAGMEPAQLADTLNKLHTRFGLNIFKPEPEIVDGSFKNMK"
     gene            5831..7732
                     /locus_tag="PLG98_09405"
     CDS             5831..7732
                     /locus_tag="PLG98_09405"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HOE33517.1"
                     /translation="MADKSKYAGVYSESINQPDIFWGKAAEDVVWNKKWDKVLDDSKK
                     PFYRWFSGGELNTCYNALDYQVESGRGEQNAIIYDSPVTNTVQKITYKELLDKVSKFA
                     GAISALGVKKGDTVIIYMPMIPEALVAVLSCARIGAVHSVVFGGFAPNELAIRIDDAK
                     PKIIISASCGIEGKKTIPYKPLLDEAIKIAAHKPQKCVIYQRPQVKADMDSARDLDFN
                     DVMKNAKAAGCVSVAATDPLYILYTSGTTGKPKGVMRDNGGHAVALKWSMKNIYDVKP
                     GDVFWAASDVGWVVGHSYIIYAPLLYGCTTIVYEGKPVGTPDPGAFWRVISQHGVSVL
                     FTAPTAFRAIKKEDPNGDYLKKYDIKCLRYLFLAGERLDPDTYHWASDMLKIPVVDHW
                     WQTETGWPIAANCMGIERFPIKAGSPTKPVPGYNVQILDGDGNQLPNGEEGSVVIKLP
                     LPPGCLPTLWNDDKRYLDYVMERPGYYVTGDGGRFDQDGYIFIMGRIDDVINVAGHRL
                     STGAMEEIISKHKDVAECAVFGAEDQLKGQVPVGFVVLKAGVDRDPDDIVRELVQMVR
                     SELGALACFKEAAIVKALPKTRSGKTLRSTMRKIVDGKEYVIPSTIDDPSVLGSIAEA
                     AKTIGYGKK"
     gene            8092..8580
                     /locus_tag="PLG98_09410"
     CDS             8092..8580
                     /locus_tag="PLG98_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33518.1"
                     /translation="MAQKITISVPDELYEKMQAWKSSLNFSRVFQNAVSKIIQKKENL
                     TSKIRNEIEFSSIVDRLKKEKVDYELNIAEWGKKDGLEWCKTAHYREIQYALAWTACE
                     NPNQDEELGDYFSDIFKKYKAQVAATGKKAQEAWSDFSEKYLKGWREGVELFWHEVKD
                     KL"
     gene            8618..9451
                     /locus_tag="PLG98_09415"
     CDS             8618..9451
                     /locus_tag="PLG98_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE33519.1"
                     /translation="MRLERETKEFFEKYRQRYLDNRQRFSDGLNKTLAEKKDWIAHIS
                     YQPYKWLFVLPLLIATTLICGVLAIVTALLFSPRTGGIFGMIWARINSFCTPMTVHVV
                     GADNIEPGTSYVITSNHQSMYDIYVLYGWLGIDFKWVMKKELKKVPVLGLACKIIGHI
                     YIDRSDSKSAVDTIKAARAKIVGGTSVLFFPEGTRSVDGKMKQFKKGAFKMAIDLGIP
                     ILPVTINGTRNILPKGSLDLTPGGATMIIHKPIPVTDYHDGNLPELMEHTRRRMNAFL
                     D"
     gene            9787..10329
                     /locus_tag="PLG98_09420"
     CDS             9787..10329
                     /locus_tag="PLG98_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33520.1"
                     /translation="MPANEPQKLPKTWTTEMLNAFHKLINSPLPVSFTVSEKNKLTTF
                     FGKQNPSSHARVACHVSYKGNISVPEFENVGVVFNPAKNLTDEYKNFIGYIEIVNGLK
                     EDTIPVPEIRVFIQEDSGVCDVFSEMLIESKTLHAKLSPIYIYLEQSYCLKDKPPEDL
                     LKKRIPIKEISTFQKISCVE"
BASE COUNT         2878 a         2622 c         2475 g         2711 t
ORIGIN      
        1 ttgcgttccc gtgtatattc caccgtcaga tcgaacgctc ttttggcaag ccccaggccg
       61 atggccgcca ccatcggacg ggcataatcc agaacatcaa tgatatattt gaaacccaga
      121 cgccggtcgc cgataatgtg attcggaaaa acgacaacgt cctcgaacgt cacgctggcg
      181 gtgttggaaa gccgttgtcc gagcttatcc tcgggtttac cgatgatgat tttaccgccg
      241 gcgggaacgc tgatttcacg ggttgcgttg ccgatatcgg ggatatcgta tgaggtgacg
      301 tgttccaccg gcacaatgac gcaggccata aaattgccgg tcggcttgcc tgttttgcac
      361 aaaacagtaa attgcgatgc gtaagacgcg ttggtgataa aacatttggc cccgttcaga
      421 atatacttac cgtcttcccg tttgctgatg aacgcagcca tcatggagtt atccgaaccg
      481 gcattgggct ccgtcagaca aaaagaggcc aggatcggct tttcgacaaa cggtttaagg
      541 aatagatttt tctgctcgtc tgtgccgtgc agtccgatga cgacgttggc cagatcgttg
      601 cacatggccg acgtagagat gcctgtatcg ccgtaggaaa gcccctcaac aaccagggcg
      661 gacgtaaaca gatccatttc ataaccgcgg acggattccg ggatgctcaa attgatcagc
      721 cccgttttcc aggccttgtt aatgatctcc cagggaaaaa cgtgctcctt ttccatgtcc
      781 agaatacgcg gcgtaatttc attcttcaca aatttgcgaa cagtatccag ataggataac
      841 tggctctcgt tgaggctgaa atccataaag cacaccttta cttattttat ttttttggag
      901 atgaacttat aaaacaactc cttttaagaa tttttcgctt caaaaaagga gtttatttcg
      961 gtttaaattt tttatgcggc cgcgcctttt ttctgggcat tgtcgtcgcc tgtcgccctg
     1021 ggcggttcga tgccatactt cgattgcaga aatttcgtca tggtcgccgg atataagatg
     1081 taggccagca catcttcgtc gctgcgtgcc aggtccccca tggccttttt cgccgcttcg
     1141 atttccggct ccagataatc ggcaggcctt ccggataccg gctcctggcc gcgcttatag
     1201 ttcttcagta ttttttttgt cagtgccgca tcaatcggca tgggtgtttt gccgtacaga
     1261 cccagagcca gatctttgag ctgattggaa actcttacgt agcggccaaa caaaacattg
     1321 agcaccgcct gcgcgccgat aatctgcgac accggcgtca ccagaggtgg tgatcccatg
     1381 tcttttctgg tctggacgac ttccttataa atttcgggca gtctgtccag ggcgttcatt
     1441 tctttgagtt ggccggtcag attggaaatc atgccgccgg gaatctgatg agcgagaacg
     1501 ccgttgtcga taatcgaaag cttatgcgta gccaggtggc tgttgtattt cggggccaca
     1561 ttttccaagt gttcgcccag cgcaagcagt ttgtacagat caatgcctgt atcgcgtgat
     1621 gtgcctctga gggcggcaac aatcggctca atggccggca tcgacgttct cagcgcaaac
     1681 ggcgccaggc acgtatctac cacatcgaca cccgcttcta tcgcttttaa atacgtcatg
     1741 gacgccatgc cgctggtgta gtgggaatgg agatgcaacg gcaggttaat ttcttttttg
     1801 atggccgtta ccagatcata ggcatcgaaa ggcgacataa tgccgcccat gtctttgatg
     1861 caaatggcat tcgcgcccat ctgttccagt gctttggctt tccggacata atagtccaga
     1921 ttgtagacgt caccacccag acggacatct gtcagcgaat agcaaaccgc tccctgaaaa
     1981 atcctgccgt ttgccttgat tcgttccgcg caggattcca tattgcggaa atcgttgagc
     2041 gcatcaaaac agcggaaaac atcaatgccg atttcacagg ccttatccac gaaagcgcgc
     2101 acgacgtcat cggcgtaatg gcggtaaccg accagattct gcccgcggat cagcatggca
     2161 aaaggcgttt tcttaatata ctgttttaaa attcggggac gcgcccaggg atcttctccc
     2221 agaaatctgt gcatggtatc aaatgtcgcg ccgccccaga cttccatcgc gtggaaacca
     2281 cactgatcca tctcctcggc gataggaatc atatcttcgg tccgcattct ggtcgcgtaa
     2341 atcgactgat ggccgtcacg gaaggtattg tcctgaatct tcaggggaat attttttatg
     2401 tctttttcct gtatcaaaat atttactcct ttgcgaaaat cattcgcgcg atggctggtt
     2461 tactgacatg ttgccttaaa aactttttct gccgcgttga gtccggtaat ggactgacag
     2521 gtaacgccta tcgtcttaaa gaaatcgcgc agcggtctgc ccggtccgat ttcataaatg
     2581 gcctgcgtat tttccgccag agcctgcata ttcttgcgcc agttcaccgt atggctgatc
     2641 tggcgaacaa ggtttttctt tacatcggcc ttatcgcccg catgaaaacc gccggtaaaa
     2701 ttcgaggtga cacggggagc gtttacggga ttcagatgac tgccgaattt ttccagcgtt
     2761 tccgcaaaac ggccctcgat ggttttcatc aacgtgctgt ggaatggagc gctcacattc
     2821 aaacgaataa aacgaaagct tttaccttcc ggaaaatttt ccgtaaaacg tttttccgct
     2881 tcgtcgatag ccggcagctc tccgctgatg accacctggt tggcggaatt gatattggcg
     2941 atgccggcct tcaggccctc catggttttg gtcagcatgt tcatatcgat gttttccgaa
     3001 atgaccgccg ccatcccgcc cacaccgacc ggcacagcgt tctgcattaa ccgtccacgt
     3061 atctgaacga ttttgagcgt atcgaacagg ggcagcacac ctgcagccac cagcgccgta
     3121 aactcgccca gcgaatgacc gccgaaataa tccggcgcga aaccatagcg ctcattcaat
     3181 ccgcgcaaca tgcagatttc cgttgtcacg atgcagggct gcgtgtactc tgtcagattt
     3241 aatctttcat cctccccaaa acacatatcc atgatattcc agcccaacgc atcggcagct
     3301 tcctcaaaag tctgccggca aaccggaatt tgctcataga aatcctttcc catacccggt
     3361 ctttgcgaac cctgccccgg gaaaacagcc gctatatttt tatcatcttt catgcagaca
     3421 aatatttcct cgttttttaa cgtgaatgta gcgtaaatcc cgcacaataa caacgacaaa
     3481 gtaaataatg taaaagcaca atttgtgcca gaaaaccggc aaccgtcaat agaacacaac
     3541 gggctgtctt tattatgctt ttaataaaat acctggtcgc gaaaccggaa aaaatccttg
     3601 cggcatgtat aaaacatttc acactgcgaa taaaagttta cctcgccgtg aacctgtaac
     3661 gaaacaaaag acgcctgcag ggcaaaaaat tcataacacc cttaatgcaa tgtccgtgtc
     3721 tttttaggct ccggcggttt tacgtcttcg acgacaggcg cgttgtattt tttattacag
     3781 ttctccatgc acaactgaaa ttgggcttcg caaagcctcc ggtcagggtc catattccaa
     3841 caaacctgct tactgtcttc gcaggacttg atgcattttt caagagaggc ttcttgctca
     3901 ttttggtttg caatggcaac cccggaaagg gacaaagacc acaaacccaa gaggttgaag
     3961 aaacaaacga tgaaaatagc ctttttcaaa gaattcatac cgaacctcct taaacgtaca
     4021 gtcctttttt aaattctgaa acggttgaag aataacccca aaacgacatt tgttctttaa
     4081 tatatacatt tgttcctgtt taggcaatca tcaaaacaag tccttttcta aacattttaa
     4141 aaggcaggct tttcttttcg ccttcgccaa aatggccttt ttcctgtttt ttgcccctgc
     4201 aggcaaggtc cctattaaat acaatattaa tcctttttcc cttgacaggt tcgccctcct
     4261 ttacctatac tccgctcaca cgaaaaagaa ttgctgccaa aattcttata taaacataag
     4321 aagaatcaca atgaaatttg gacaggaagt tcttattaat tcaattaaga agaaggaggt
     4381 cggtaatgaa gttagatgat atcaagaaaa ttgcagtaat tggttcagga gctatgggga
     4441 acggcattgc ccaggtatgc gcacaggccg gcatcaaggc cgtcatggtg gacatcaaac
     4501 aggagtttct cgacaaaggt atggccacgg tcaacaagag tctggatact ctggtcggca
     4561 agggtaaaat gactgctgag aaaaaggccg aagtcatggg caatttgtcc acatctttaa
     4621 gcaataccga ggcagtcaag gacgttcagg tgattatcga agccgttccg gaaatcatgt
     4681 ctctgaagaa aacagtgtgt gccgaaatat cggctgcggc tccggctgac gcgctgatag
     4741 cgtccaacac ttccaccatg agcattagcg aaatcgccac ggccgggaag aacccggagc
     4801 ggattctcgg tatgcatttc ttcaatcccg cgccggtcat gaaaggcgta gaagtgattt
     4861 acgccaagca aacctctgat gccaacgtgg acctgctttg cgaactgacc aagaaaatcg
     4921 gaaaagttcc catcaaagtg ctcaaggact ccccgggctt tatcgtgaac cgcatcggag
     4981 cacccaacca ggctctgatc agcgccattc tggatgaagg caaaatcaaa gtggacacga
     5041 ttgatgctgt catgaaggcc aaggccggta tgcccatggg tcccttcgag cttgctgact
     5101 acgtcggtat tgacgttttc tatcacactc tcaaatatta ttccgaaaca ctgtctccgg
     5161 aattcaaacc cggcaagaca cttcagactc tgttggacag cgggaaactc ggcatgaaga
     5221 gcggccaggg catttacacc tgggaaggcg gcaaggccaa gatcgatatg tccgtagaat
     5281 ctcaggactt cggtccgctg gattttctgg ccattcagat caacgaagcc gtgcgtgttt
     5341 acaaagagaa gattgcggca tccgctgccg atatcgacct gggcatggtc cattgcatga
     5401 gagcgtttgc cggtccgttc gcctttggcg ccggcatgga acccgctcaa ctggcagata
     5461 cgctcaataa actgcacacc cgtttcggtc tgaatatttt caaacccgaa cccgaaatcg
     5521 tggacggctc ctttaaaaac atgaaataaa cagttttgtt ttatctccga cagaggggca
     5581 tcctctgccg accaatccaa tgaggcgggg caacgaaata tcgttgcccc gcttttttta
     5641 gtcaggcttc aattttaaag catcgcgtat ggacattggc cgatcgaatt atcccctgag
     5701 cacagcaaac ggaaatctgt ccgttgaagc gggattcaag tctcacgaaa gaaatgatct
     5761 ttatctttga caaattcgat ttcaatctgt tatgcccata gcaaatctat tcatttaaag
     5821 aggactcatc atggctgaca aatcaaagta tgcgggagtt tattctgaat caatcaatca
     5881 accggacatt ttctggggca aagcagccga agacgtcgtc tggaacaaaa aatgggacaa
     5941 ggttcttgac gatagcaaaa aaccttttta ccgctggttt tccggcggcg aattgaacac
     6001 ctgctataac gcccttgatt accaggtgga atcgggacgg ggggaacaaa acgccattat
     6061 ttatgacagc cccgtaacca ataccgtgca gaaaattacc tacaaagaac tgctggacaa
     6121 agtatccaaa tttgccggag ccattagtgc cctgggcgtc aaaaaaggcg ataccgtcat
     6181 tatctacatg cccatgattc cggaagccct ggttgctgtg ctttcctgcg cacgcatcgg
     6241 cgcggttcat tccgttgttt tcggtggatt tgctcccaac gaactggcca tacgcattga
     6301 tgatgcaaag ccgaaaatta tcatctccgc atcctgtggc atcgaaggga agaagaccat
     6361 tccttataaa ccgctactgg atgaggctat taaaattgcg gctcataaac cgcagaaatg
     6421 cgttatctac cagcgtcccc aggtcaaggc ggacatggat tcggcacgcg atctggactt
     6481 taacgacgtg atgaaaaatg ccaaagcggc gggctgcgta tccgttgccg cgaccgatcc
     6541 cctctacatt ctctatacgt cgggaacaac cggcaaaccc aaaggggtca tgcgcgataa
     6601 cggcggtcat gccgtggcgc tgaaatggtc tatgaaaaat atatacgatg tcaaaccggg
     6661 cgatgttttc tgggccgcct ccgatgtcgg ctgggtggtc ggccattctt acattattta
     6721 tgcaccgctt ctttatggct gcacaaccat cgtctacgaa ggcaaaccgg tcggaacacc
     6781 ggatccaggt gctttttggc gcgtcatttc ccagcatggc gtctctgtgc tatttacggc
     6841 tcccacagcg ttccgcgcca tcaagaaaga agaccccaac ggcgactatc tgaaaaaata
     6901 cgatatcaaa tgccttaggt atctttttct ggcgggcgaa cgactcgatc ccgataccta
     6961 tcactgggcc agcgatatgc tgaaaattcc tgtggtggat cactggtggc agacggaaac
     7021 cggatggccg attgccgcca actgcatggg cattgaaaga ttccccatca aggcgggctc
     7081 accgaccaaa cccgtcccgg gttacaacgt gcagattctg gacggggacg gcaaccagct
     7141 tcccaacggc gaagaaggtt ccgtggttat caaacttcct cttcctccgg gctgtctgcc
     7201 cacgctttgg aacgatgaca aacgttatct ggactacgtc atggaaagac ccggttatta
     7261 tgtgaccggc gacggcggac gctttgatca ggacggttat atttttatca tgggacgcat
     7321 cgacgacgtc atcaacgtcg cagggcatcg cctgtctacc ggagcgatgg aagaaatcat
     7381 ttcgaagcac aaggatgtcg cggaatgcgc agttttcggc gccgaagatc agctcaaagg
     7441 ccaggttccc gtaggctttg tcgtgctcaa agccggcgtc gaccgagatc cggacgatat
     7501 cgtcagggaa ctggttcaaa tggttcgttc agaacttggc gcgctggcct gcttcaagga
     7561 agcggccatc gtcaaggcac tgccgaaaac gcgttcagga aaaactctgc gcagcacgat
     7621 gcgcaagatc gtggacggca aggagtatgt gattccttct acgattgacg atccctcggt
     7681 tctgggaagt attgcagaag ccgctaaaac catcggctac ggaaaaaaat aatcgtattc
     7741 gtttttgcat gcgcgcccct atgttcgggc gcgcattttt ttcgcagctg ttgcctcggg
     7801 ttcataccgt taaaaacaca atttagcttt tttgcatttc aatcagccag cagcatgccg
     7861 tcgcttgatc tgttttctgc tggcgccatt tagattttac cctcaaattt atcgttacca
     7921 cttttacgca aaattgttga aagctgattg ttctatcgat cagcgtaaac tgcttcaact
     7981 ttctgaattg gttgcggcat tatattcgca caacaacttg cagttgacac atccttttta
     8041 atcatatatg tactatttaa aatatatgta ttaaattttg gagtatgtat tatggcgcaa
     8101 aaaataacga tcagcgttcc ggacgagctt tatgaaaaaa tgcaggcatg gaaatcgtct
     8161 ctgaattttt ccagagtatt tcagaatgcc gtttccaaaa tcatacagaa aaaagagaat
     8221 ttaacatcga aaatccggaa cgaaatcgaa ttttcatcta tcgtggatcg cctgaaaaag
     8281 gaaaaggtcg attacgagct caacatcgcg gaatggggga aaaaggacgg cctcgaatgg
     8341 tgcaaaaccg cccattaccg tgaaattcaa tatgccctcg cctggaccgc ttgcgaaaat
     8401 cccaaccagg atgaagagtt aggcgattat ttctccgaca tatttaagaa atataaggcg
     8461 caagtggcgg caacgggcaa aaaagcacag gaggcttgga gtgacttttc ggaaaaatac
     8521 ctgaagggat ggcgagaagg agtcgaatta ttctggcacg aagtgaagga caagctttga
     8581 tcatcagaaa aacgctgcaa tctccaccgg gggatttatg agattggaac gcgaaacaaa
     8641 agaattcttt gaaaaatacc ggcagagata tctggacaac cggcaaagat tttccgacgg
     8701 cttgaacaaa accctggcag agaaaaagga ctggatcgcc catatcagtt accagcctta
     8761 taaatggctc tttgttctgc ctttattgat tgcgaccact ctgatctgcg gcgttttggc
     8821 cattgtcaca gcgctgctgt tctcaccgcg cacaggtgga atttttggaa tgatttgggc
     8881 cagaatcaac agcttctgca cccccatgac tgtacacgtc gtcggagcgg ataacatcga
     8941 acccggcacg tcttacgtca tcacttccaa tcaccagagc atgtatgata tctatgtgct
     9001 ttacggctgg ctgggcatag acttcaaatg ggtgatgaag aaagagctga aaaaagtgcc
     9061 ggttcttggt ttggcctgta aaattatcgg tcacatttac atcgacagat ccgactcgaa
     9121 aagtgccgtg gacaccatca aagccgccag ggcaaaaatt gttggcggaa cctccgtttt
     9181 gttttttccg gaaggaacgc gaagcgtcga cgggaagatg aaacaattta agaaaggcgc
     9241 ctttaagatg gccattgacc tcggcatacc cattcttccc gtcaccatca acggtacccg
     9301 aaacattctg ccgaaaggca gcctggattt gacccccggc ggtgcgacga tgatcattca
     9361 taagccgatt cccgtcacgg attatcacga cggaaacctg cctgaactga tggagcacac
     9421 aaggcggaga atgaacgcat tcctggattg aggcagtgag cgccggcagc accaaatctg
     9481 agataaacac tgtcctgaat aatgctgcaa acatcaagct ttcgatttaa tatccccctg
     9541 cgcggtaaaa aaaactattt cattgaaata ttcttgaaga aaggttatga ataaaataaa
     9601 tgttgaaatg atttctcagt agtctagtgc tcataaaact ttcaaatggc atgagtggat
     9661 tagatgaacc atacggggga aaatccaaaa cccactttct cgcacagaga aaatgggttt
     9721 tctttttcgg gaatgcatta ttgaatttgc agctacatta atctctaaca aaaaaggatt
     9781 aaaattatgc cggcaaacga acctcaaaaa cttcccaaaa catggacaac cgaaatgttg
     9841 aatgcgtttc acaaattgat caattcgccc ctacccgtca gttttacggt ttcggaaaaa
     9901 aataaattga caactttttt cgggaaacaa aatccgtcgt ctcacgcacg cgttgcctgt
     9961 catgtttcct ataaagggaa tatttccgtt ccggaatttg aaaatgttgg cgtcgttttt
    10021 aatccggcaa agaatttgac agacgaatat aaaaatttca tcggctatat tgaaatcgta
    10081 aacggtttga aagaggatac gattcccgtc ccggagatca gagtctttat tcaggaagat
    10141 tcgggggtgt gtgacgtttt cagcgaaatg ctgattgaat caaaaacatt gcatgcgaaa
    10201 ctctccccca tctatattta tctggagcaa tcttattgct taaaagataa gcctcctgaa
    10261 gatctgttaa agaaaagaat cccgatcaag gagatatcca catttcaaaa aatttcctgc
    10321 gttgaataat ctaaaaacac gcggggaagc cggcttttct gatattcacc atgcaccgac
    10381 tcatcagtgc cccttcagcg taagcgtaaa ggcgtctgaa aaggacaacc tcgaccccct
    10441 aaaaagggga agttgtctgt ttgtggattt ttaccccctg attgatacga tcataccttc
    10501 tgccattttt gaaagccgtt atgattcatg tgaacggagt gattgaaatt gaaaaactaa
    10561 tttccaaata taactatcgc cacgccgtat ctgatcaaca agaatcctct tgaaacgtcg
    10621 caggcaatcc acctgttgcc tcattaataa atgttaccga aagaagagcg agtggagaga
    10681 acgttg
//
LOCUS       DAOSME010000071        10213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_7471_length_10213_cov_21.047450, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000071 DAOSME010000000
VERSION     DAOSME010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7471_length_10213_cov_21.047450"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2117
                     /gene="topA"
                     /locus_tag="PLG98_09425"
     CDS             <1..2117
                     /gene="topA"
                     /locus_tag="PLG98_09425"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOE33521.1"
                     /translation="IYLAPDPDREGEAIAWHINEVLNTKNKNVYRVLFNDLTKDTVTE
                     AIKNPLQLDFNKYEAQQTRRILDRLVGYQISPVLWDKVKRGLSAGRVQSVAVRMICDR
                     EDEIKNFKPEEYWNLTAKFEGKNPPPFEAKLHKVDGKKAKVTNGEQAAMLADKIKQAE
                     FFVEKLEKKEVKRTAPPPFTTSKLQQEASRWFRFSAKKTMMVAQKLYEGIELGKEGSV
                     GLITYMRTDSYRISEEAIKDVRDYIRESYSADYLPHKPHTYKNTQKAQDAHEAIRPSR
                     MTNKPQDIKQYLSADQFKLYQLIWNRFVASQMNPAILDQTTIDIAGANCLFRAQGQIM
                     KFPGFTIVYTEGKDDKDENGNGNGNGTDKFLPEVSEKEKLKLLHLNTEQKFTQPPPRF
                     SEASLVRELEEKGIGRPSTYAAILSTIQEREYVKLEKGKFYPSELGMMVTHLLVKSFP
                     TILEIAFTADMENKLDAIETGERKRVQTLKEFYALFADELKKAKAEMKNIKQEETPTD
                     LVCEKCQSPMVIRWGKNGKFLCCSNYPKCKNTMNFTHDENGKVKHVETRTTDIKCNKC
                     GKNMIVKEGRFGQFLACSGYPECKNTMTAAKDENGEIVAQATPTTDEVCELCGKPMTV
                     KRGRYGQFLGCTGYPECKNIKKLDKDGKAAKQEVVLSDEVCELCGKPMAVKRGRYGQF
                     LGCTGYPECKNIKKIPKTKTES"
     gene            2117..3460
                     /gene="trmFO"
                     /locus_tag="PLG98_09430"
     CDS             2117..3460
                     /gene="trmFO"
                     /locus_tag="PLG98_09430"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOE33522.1"
                     /translation="MDHRVTIIGGGLAGCEAAWQLLRRGHAVEMYEMKPQKFSEAHKM
                     ENLAELVCSNSLKSNSLDNAPGLLKEEMRLLDSVIMKAADKTAVPAGSALAVDRLLFA
                     REVEARLSEQAAFTLYRHEVTAIPNDTVTIIATGPLTSDAMAHDISRRLGGASLYFYD
                     AIAPIVEADSIDMNKVFWASRYDKGTPDYLNCPLTREEYESFHKALLAGEKVAARDFE
                     ETRHFESCLPIEILAQRGEKSLAFGPMKPVGLIDPHTGNKPYAVVQLRRENAAGTLFN
                     LVGFQTKLTWPEQRRIFRLIPGLEKVEFARYGSIHRNTYINSPALLSPTLQLKNHENI
                     FFAGQITGVEGYMESAAMGLLAGLNAAFIIEGKKAEPPPVNTALGALIQYITSPEHAV
                     QFQPMNINFGLFNAPVEKKLRKKERNALIVHQALQSLNAWISSQKLNELRPDIFC"
     gene            3557..4291
                     /locus_tag="PLG98_09435"
     CDS             3557..4291
                     /locus_tag="PLG98_09435"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOE33523.1"
                     /translation="MPEQNRENSLVLIKPDAIKNSLTGYILSRFSEFHTGLRFAALKV
                     VAVNLALAEEHYAEHKGKFFYPSLLEYIRGYLHYPDEPSKRRVIAIIYRGPNAIKNIR
                     EICGNTNPHEARAQRPGCIRSLGTIIPLYDAKGKFIGDRSDNLIHASANIQDAEREIK
                     LWFLPTDIPPTVRSYPAETSKEYYYYKNGAISSEYTPGSYCFIGPGDLVWKSDMKVLR
                     QIAKGKKQDYSTNAVIAKYLINKETD"
     gene            complement(4387..4854)
                     /locus_tag="PLG98_09440"
     CDS             complement(4387..4854)
                     /locus_tag="PLG98_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33524.1"
                     /translation="MRRVSLLLLILTVALLFSACGKDEPELSCIYPGLYEINHDIKYS
                     GQLVILKQRVRYHADGTFEATNFQNNAALEEMKGKYKVENKQLISYDTQRRLITQEGT
                     WTPPKKEPSAVELRKIKKGSYQYYFKYPDEQMREKYKGLGLAEGWKTYKRISD"
     gene            5157..5828
                     /locus_tag="PLG98_09445"
     CDS             5157..5828
                     /locus_tag="PLG98_09445"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOE33525.1"
                     /translation="MRGKVIIVSGGESTDAAFFNSQAGKRDDCLIIGCDGGSRHLKSW
                     GIKPDVIIGDMDSIDAALLAYYDSENIKIIKYPANKDFTDTELALDYALEVKPEKIYI
                     WCALGGRMDHTLSNIFLLFKGRERGIQTVLIDPFCEIFLADNETHFTGDTGKTVSLLS
                     LSPEVTGITLSGFLYPLENENLKMGESRGISNRIRDNDACIRFANGKLLVIKYQNKDR
                     FPEAV"
     gene            5831..6631
                     /gene="thiD"
                     /locus_tag="PLG98_09450"
     CDS             5831..6631
                     /gene="thiD"
                     /locus_tag="PLG98_09450"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOE33526.1"
                     /translation="MKLRKVLCIGGSDSSGGAGIQADLKAVSACGCWGLTVVTAVTAQ
                     NTKGVSGIYPVDPAFVAKQMDVVLSDIGVDAIKTGMLPTEEIIQVVVKKIKKYKLKNV
                     VVDPVMIAKGGRTLMTGKARQMLVQTLLPLALVITPNIPEAETLSKNKIKTMADMKDA
                     AAVIYESGAANVVIKGGHLPDRKHADVTDILYSGSGYCEFSSARIRSCHTHGTGCTFA
                     SGLASGLARRKSLADAVLQAKITVADAIRKAVVLGHGYGSVNVFEKFS"
     gene            6674..>7232
                     /locus_tag="PLG98_09455"
     CDS             6674..>7232
                     /locus_tag="PLG98_09455"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOE33527.1"
                     /translation="MTQLEKARKGIITEAMKAAAKEEKVAPEYIRKGIAEGTIVLCRN
                     VKHPSIKPLAIGRGLRTKVNANIGTSKDHTDLNLELKKLKIAVDAGADAVMDLSTGGN
                     LAAIRKKVMKKSTVAIGTVPIYQAAVKMLQDRKAISEMTADNIFDVIEENGRDGVDFI
                     TVHCGVTRLSVSALKSQKRILGIVSR"
     assembly_gap    7233..7332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7333..8100
                     /gene="thiC"
                     /locus_tag="PLG98_09460"
     CDS             <7333..8100
                     /gene="thiC"
                     /locus_tag="PLG98_09460"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOE33528.1"
                     /translation="LSVSALKSQKRILGIVSRGGSMTANWMDCNKKENPLYEEYDRLL
                     EIAHRYDMVLSLGDGLRPGAIDDATDQAQLQELIILGALAARARAAGVQVMIEGPGHV
                     PLTDIVTNIRLQKDICQNAPFYVLGPLPTDIAPGYDHITSAIGGAIAGAAGADFLCYV
                     TPAEHLRLPTLNDVRDGVIAARIAAHIADIAKGVKQAREKDRKMSSCRKNFDWQGQVD
                     LSIDPEKTVSLLEKSKSAKNEGCTMCGEFCAIKLGKR"
     gene            8119..9456
                     /locus_tag="PLG98_09465"
     CDS             8119..9456
                     /locus_tag="PLG98_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOE33529.1"
                     /translation="MPDHAHRLHAREIIHRLRESGHEAYFVGGCVRDFILNQTSSDYD
                     IVTSARPEEVMKLFPRTVAIGAKFGVVAVIIDNHPFEVATFRSDDVYEDGRHPSRIHY
                     STAREDVFRRDFTINGLLMDPDTGDIIDYVNGRFDIDNKIVRTIGNPEKRFREDYLRL
                     LRAVRFAANLHFDLEPATQDAVKREASNIRHISVERIREELNKILTRGGARRGFELMM
                     QTDILQNILPEICRLKGVEQPPRFHPEGDVWQHTLKMLELLPADGEREKNICLAWGTL
                     LHDVGKALTRSEDEKGVHFYGHVQQGEIIADTIMQRLKFSRMQRETVVNLIRQHMVFM
                     NVRKMRPGRLKRFLRMPDFDLHLELHRLDCLASHGILDNYEFCLEQMQILALDDLHPP
                     RLLTGNDLIAMGFTPGKIIKDILLAVEEEQLEGRIQSAEQAINYVRNRWNIES"
     gene            9453..>10213
                     /locus_tag="PLG98_09470"
     CDS             9453..>10213
                     /locus_tag="PLG98_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOE33530.1"
                     /translation="MKKYRLIICLFLLFTLMLACSGKAHHRRVASAPRYKPALNLMGY
                     TIQAGAFRNVENAVRLTERLKINHGLDATYFLADDKFFKVRFGNFSTKDLAKKRALEL
                     IDAKVIEEFYIVRPEDYSVARQKQYGTDYLRESIVTTARDFLGVPYLWGGSSPDDGFD
                     CSGLTMTVYQLNGLNLPRNSRKQYAVGNAIEKNELQKGDLVFFADRGNHHVSHVGIYV
                     GDNQFIHASSQGNEIRIDHLSSDYFTRQYVGARTY"
BASE COUNT         2753 a         2535 c         2535 g         2290 t
ORIGIN      
        1 acatttatct ggcccccgac ccggaccgcg aaggcgaagc cattgcctgg catattaatg
       61 aagttctcaa caccaagaac aaaaacgtct accgcgtcct cttcaacgat ttaaccaaag
      121 ataccgtgac cgaggccatc aaaaatcccc tgcaactgga tttcaacaaa tatgaagcgc
      181 agcagacccg ccgcattctg gaccgtctgg tcggttatca aataagtccg gtgctgtggg
      241 acaaggtcaa acgaggcttg agcgcggggc gcgttcagtc cgtcgccgtc cggatgatct
      301 gcgaccggga ggacgaaata aaaaatttca agcccgaaga atactggaat ctgacggcca
      361 aattcgaagg taaaaatccg cctccttttg aagcgaaact tcataaagtc gacggcaaaa
      421 aagcaaaagt gaccaacggc gaacaggccg cgatgcttgc cgataaaatc aagcaggcgg
      481 agttttttgt tgaaaaactg gaaaagaaag aagtcaagcg cactgcgccg cccccgttta
      541 ccaccagtaa attgcagcag gaagcatcgc gctggtttcg tttttcagcc aagaagacca
      601 tgatggtcgc gcaaaaactc tatgaaggaa tcgaactggg caaggaaggt tccgtgggtc
      661 tcatcacata tatgagaacg gactcctacc ggatatccga agaagccatc aaagatgttc
      721 gcgactacat cagagaaagt tattccgcgg attatctgcc gcataagcct catacgtaca
      781 aaaatacgca aaaagcgcag gatgcccatg aagcgattcg tccgtcccgc atgactaata
      841 aaccgcagga catcaagcaa tatctgagcg ccgatcaatt caaactttat caactgatct
      901 ggaaccgctt tgtcgccagt cagatgaatc cggccattct cgatcagacc accattgaca
      961 ttgccggcgc caattgtctt ttccgcgcgc agggacagat catgaaattc cccggcttca
     1021 ccatcgtcta tacggaaggc aaggatgaca aggatgaaaa cggcaacgga aatggcaacg
     1081 gaacggacaa gtttttgccg gaagtcagcg aaaaagaaaa gttgaaactt ctccatctga
     1141 atacggaaca gaaattcaca caaccgccgc cgcgtttttc cgaagcgtcg ctggtgcgcg
     1201 aactggagga aaaaggcatt ggacgcccca gcacctatgc cgcgattctt tccacaatac
     1261 aggaacgcga atacgtgaag ctggaaaaag gcaagtttta tccgtcggaa ctgggaatga
     1321 tggtgacgca tcttctggta aaaagttttc cgaccattct ggaaatcgct ttcaccgcgg
     1381 atatggaaaa caaactggac gccattgaaa cgggcgaacg caagcgtgtg cagacgctca
     1441 aggaatttta cgcgcttttt gccgacgagc tgaaaaaagc caaggcggaa atgaaaaaca
     1501 tcaagcagga agaaacgcca accgatctgg tctgcgagaa atgccagtcc cccatggtga
     1561 ttcgctgggg gaaaaacggg aagtttctgt gctgttccaa ttatcccaaa tgcaaaaaca
     1621 ccatgaattt tactcatgac gaaaacggaa aggtcaaaca cgtggaaacc agaacgacag
     1681 atatcaaatg caacaagtgc ggcaaaaata tgattgtcaa agaggggcgt ttcggtcagt
     1741 tcctcgcctg ctccggttat cccgaatgca aaaacacgat gaccgcggcc aaagacgaaa
     1801 acggcgaaat cgtcgcacag gcaacgccga cgaccgatga agtctgcgag ctgtgcggca
     1861 aacccatgac ggttaagcgc ggccgctacg gccagttcct cggctgcacc ggttacccgg
     1921 aatgcaaaaa catcaaaaaa ctggacaagg acggaaaggc ggccaaacag gaggtcgttc
     1981 tgtcggatga agtctgcgag ctgtgcggca aacccatggc cgtcaagcgc ggccgctacg
     2041 gccagttcct cggctgcacc ggttatcccg aatgcaagaa catcaaaaaa atccctaaaa
     2101 caaaaacaga atcctgatgg atcatcgtgt gacaattatc ggcggcggac tggccggctg
     2161 cgaggcggcc tggcagctgc tgcggcgcgg ccatgcggtt gagatgtatg aaatgaaacc
     2221 gcagaaattt tccgaagccc acaaaatgga gaatctcgcc gaactggttt gcagcaattc
     2281 cctcaagtcc aactctctcg ataatgcgcc cggtttgctc aaggaagaaa tgcgcctgct
     2341 ggactcggtc attatgaaag cggcggataa aaccgccgtg cccgccggat ccgctctggc
     2401 ggttgaccgc cttctgttcg cccgcgaggt ggaagcccgg ctttctgaac aagcggcctt
     2461 caccctttac cggcatgaag taaccgccat cccgaacgat actgtaacga tcattgccac
     2521 aggcccgctg acctccgacg caatggcgca tgatatttcc cggaggctcg gcggtgcgtc
     2581 gctctatttt tacgatgcca ttgcgcccat tgtcgaagcg gattccattg atatgaacaa
     2641 ggtgttctgg gcgtcgcgct atgacaaggg aacgcccgac tacctgaatt gccctctgac
     2701 ccgcgaagaa tacgaatcat ttcacaaggc gttgctagcg ggagaaaagg tggccgccag
     2761 agattttgag gaaacccgcc atttcgagag ctgccttccg atagaaatcc tggctcagcg
     2821 cggcgaaaag agcctggcat tcggtccgat gaaaccggtg ggcctgattg atcctcatac
     2881 cggcaacaaa ccctacgccg ttgtgcaatt gcgcagagag aacgccgccg gcactctttt
     2941 taatctggtc ggtttccaga ccaaattgac ctggccggaa cagcggcgga tttttcgcct
     3001 gattcccgga ctggagaagg ttgaatttgc ccgctacggc agcattcacc gcaacaccta
     3061 tatcaactcc cccgccctgc tgagccctac cctgcagctc aaaaaccatg aaaatatttt
     3121 ctttgccgga cagatcacgg gcgtggaagg ctatatggag tcggcagcca tggggcttct
     3181 ggccggtctg aacgcggcgt ttatcatcga agggaaaaaa gccgaaccgc ctcccgtcaa
     3241 cacagcgctg ggcgctctca ttcaatatat cacatctccc gaacatgcag ttcagtttca
     3301 accgatgaac attaatttcg gactgttcaa cgcacccgtg gaaaaaaaac tacggaaaaa
     3361 agagagaaac gccctcatcg tccatcaggc tctacagtcc ctgaatgcgt ggatttccag
     3421 ccaaaagctc aatgaacttc ggcccgatat attctgttga cacaaagcag gacttctctt
     3481 atacttcgcc cgctttttaa gcatgtcttc tcacaaagaa gattgttaac atctttaacc
     3541 ataaggagag tttcaaatgc ctgaacaaaa tcgagaaaat tcattggttc tgataaaacc
     3601 ggatgcaata aagaattccc taaccgggta tatcctttca cgattttcag agttccacac
     3661 cggactgcgt ttcgcggcgc tgaaggttgt cgccgtcaat ctggcgctgg cagaagaaca
     3721 ttacgccgaa cacaagggaa aatttttcta tccgtctttg cttgaataca ttcgcgggta
     3781 cctgcattat ccggatgaac cctcgaagcg cagagtcatc gcgattatct accggggacc
     3841 gaacgccatc aaaaacatca gggagatttg cggtaacacc aatccgcatg aagccagagc
     3901 gcaaagaccc ggctgcatcc gctcactggg aacgatcatt ccgctgtatg acgcaaaggg
     3961 aaaatttatc ggagatcgat cggacaatct gattcatgca tccgcaaata ttcaggatgc
     4021 tgaacgtgaa attaaactct ggtttcttcc cacggatatt ccgcccacgg tacgcagtta
     4081 tcctgcagag acaagcaaag aatactatta ttacaaaaac ggcgcgatca gttccgaata
     4141 caccccggga agctactgtt tcatcggacc cggcgatttg gtctggaaat ccgacatgaa
     4201 agtcttgcgg cagattgcaa aaggcaaaaa acaggattat tcaacgaatg cggttattgc
     4261 caaatatcta atcaataagg aaacagacta aactcagagg ttttcgccgg caccgcaatt
     4321 cgccgaaata ttgtttgacg gttttttcat tgtcctcagc cggatggctg tccggttgtc
     4381 cggcaactaa tcgctgatgc gcttgtacgt cttccagcct tctgccaggc cgagtccctt
     4441 gtatttttca cgcatttgct catcaggata tttgaaataa tactggtagc ttcccttttt
     4501 gatcttgcga agttccacgg ccgagggttc ctttttcggc ggtgtccacg tcccttcctg
     4561 agtgatcagg cggcgttgtg tatcgtaaga aatcagctgc ttgttttcta ctttatattt
     4621 gcccttcatc tcctccagag cagcgttgtt ctgaaaattc gtggcttcaa acgtgccgtc
     4681 ggcatgatag cgaacacgct gtttcaggat caccagctgg cctgaatatt taatatcatg
     4741 attgatttcg taaaggcccg gatagatgca ggacagttcc ggttcatctt taccgcaggc
     4801 gctaaaaagc aacgccacgg taagaatcag taacaataat gaaacccttc gcatagaatg
     4861 tcagctcctt tatcggataa tccctgttgt ttttagaagt gattttattt tttatttttt
     4921 taaactataa tatttacagg gtgttaaaaa acaaacaacg gtgcttttgt caagacttaa
     4981 tttgtgacgt ttttcatgat ggtcctgaaa agcggtatcc aaaaacgatt ttacatcaga
     5041 ggatcaaaca atttgctttt taaagttgaa tataataaat tttctacaag tatacgaatg
     5101 acttaattta taaagccgga tatccttaaa cgggtgtcgc cattcaggta aatcacgtgc
     5161 gcggaaaagt aatcatagtc agcggcggcg aatcgaccga tgccgcattt tttaacagcc
     5221 aggccggcaa gcgggatgat tgtctgatca tcggctgcga cggcggatcc cgtcatctca
     5281 aaagctgggg catcaaaccg gacgtcatca tcggggacat ggattcgatc gacgccgctc
     5341 ttctggctta ttatgattcg gaaaacatta aaatcataaa atatccggca aacaaagact
     5401 ttaccgatac ggaactggcc ctggattacg cgttggaggt aaaaccggaa aaaatatata
     5461 tctggtgcgc tctgggaggc aggatggatc atacgctgtc caatattttc cttcttttta
     5521 aagggcggga aagaggaatt caaacggtgc tgatcgaccc gttctgcgaa atatttctgg
     5581 cggataacga aacgcatttt accggcgaca cagggaaaac cgtatccctg ctatcgctga
     5641 gtccggaggt taccggaatc accctttccg gatttctgta tcctctggaa aatgaaaatt
     5701 tgaaaatggg ggaatcccgc ggtatcagca accggatcag ggacaatgat gcgtgtatcc
     5761 ggtttgcaaa cggcaagctt cttgttatta agtaccaaaa caaggatcgt ttcccggagg
     5821 cggtttaatc atgaaattgc gcaaagtctt atgcatcggc ggttccgact ccagcggcgg
     5881 cgccggaatt caggcggatc tcaaagctgt cagcgcctgc ggctgctggg gattgactgt
     5941 agtcacggcc gttacggcac aaaacacaaa aggggtgtcg ggaatttatc cggttgatcc
     6001 ggcatttgtc gcaaagcaga tggatgtcgt gctgagcgat atcggagtcg acgcgatcaa
     6061 gaccggcatg cttccgaccg aagagattat tcaggtggtc gttaaaaaaa ttaaaaaata
     6121 taaactgaaa aacgttgtgg tggatcccgt gatgattgcc aaaggcggca gaactctgat
     6181 gaccggcaaa gcccgacaga tgctggtgca gacgcttctc ccgctggcgc tggtcattac
     6241 gccgaacatc ccggaagcgg aaacgctctc gaaaaataaa attaaaacca tggccgacat
     6301 gaaggacgct gccgcggtca tttacgaatc cggcgcggca aatgtcgtca taaaaggcgg
     6361 acatctgccc gaccgcaaac atgccgatgt gaccgacatt ctgtacagcg gatccggcta
     6421 ctgcgaattt tcctctgcca gaatccgttc ctgtcatacg cacggcaccg gctgcacctt
     6481 cgcttcgggg ctggcgtccg gtctcgcccg caggaaaagt cttgcagacg ctgtgttgca
     6541 ggctaaaatc acggtagccg acgctatccg gaaggcggtt gttttgggac acggttacgg
     6601 atcggtcaat gtttttgaaa aatttagtta atatttggcg atataaaatc gctttcacat
     6661 ttcggaggat acgatgacac aattagaaaa agcccgtaaa ggcatcatta ctgaagcaat
     6721 gaaggcagcg gcaaaagaag aaaaagttgc tcccgaatat atccgcaaag gaattgccga
     6781 aggcaccatt gtcctttgcc gcaatgtaaa acatccgtcc attaaaccgc tggccatcgg
     6841 ccggggtctg cggacgaaag tcaacgccaa tatcggcacc tccaaagacc ataccgattt
     6901 aaatctggaa ttgaaaaagc tgaagattgc cgttgatgcg ggcgccgatg cggttatgga
     6961 tttatccacc ggcggcaatc tggccgccat cagaaaaaaa gtcatgaaaa aatcaacggt
     7021 ggcaatcggg accgtcccga tttatcaggc ggcagtcaaa atgctgcagg acaggaaagc
     7081 catatcggaa atgacagcgg ataatatttt tgacgtcatt gaagaaaacg gcagagacgg
     7141 cgtggatttt attacggtcc actgcggcgt cacccggttg agcgtctcgg cgctgaaatc
     7201 acaaaaaaga atcctgggca tcgtcagccg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnttgagcgt ctcggcgctg aaatcacaaa aaagaatcct gggcatcgtc
     7381 agccgcggcg gttcgatgac ggccaactgg atggactgca ataaaaaaga aaacccgctt
     7441 tatgaggaat acgaccggct gctggaaatt gctcatcgtt acgatatggt gttgagcctc
     7501 ggcgacggtc tgaggcccgg agctattgac gacgccaccg atcaggcaca acttcaggag
     7561 ttaatcatcc tcggggcgct tgccgcacgc gcgcgcgccg ctggcgtgca ggtgatgatt
     7621 gaaggacccg gccatgttcc cctgacggac atcgtcacca acatcagact gcaaaaagat
     7681 atttgtcaga acgctccttt ttacgtgctg ggccctctgc ccacggatat cgcacccggc
     7741 tacgatcaca ttacgtcagc catcggcgga gcgattgccg gcgccgccgg cgccgatttt
     7801 ctgtgctacg tgacgcctgc ggagcatctg cggctgccga ctttgaacga tgttcgcgac
     7861 ggcgttattg ccgcgcgtat cgccgcacac attgccgata ttgccaaggg cgtgaagcag
     7921 gcgcgtgaaa aagaccggaa gatgtcttcg tgccggaaaa atttcgactg gcagggacag
     7981 gtggatttgt ccatcgatcc tgaaaaaacc gtttcgcttc tggaaaaaag caaaagcgcc
     8041 aaaaatgaag gctgcacgat gtgcggcgaa ttctgtgcga taaaattagg taagagataa
     8101 tcaccagagg ataaattatt gcctgatcat gcccatcgcc ttcatgccag ggaaattatt
     8161 caccggctga gagaatccgg tcacgaggca tattttgtcg gcgggtgtgt tcgcgatttt
     8221 attctcaacc agacttccag cgattacgat attgtaacgt cggcgcggcc ggaggaggtc
     8281 atgaaactct ttccccgcac cgtagccatc ggcgccaagt tcggcgttgt tgccgtcatt
     8341 atcgacaatc atccttttga ggttgccacg tttcgcagcg atgatgttta cgaagacggc
     8401 cggcaccctt cccggattca ctattccact gcccgggaag atgttttccg gcgcgatttt
     8461 acgatcaacg gccttttaat ggatccggat accggagata ttattgatta tgtcaacggc
     8521 cgttttgata tcgacaataa aattgtcaga actatcggca atccggaaaa acggttccgt
     8581 gaggattatc tgaggttgct ccgggcggtg cggtttgcag ccaaccttca tttcgacctg
     8641 gaaccggcga cacaggatgc cgtcaaacgc gaagcttcga atatccgaca catcagtgtt
     8701 gaacgcatcc gggaagagct caacaagatt ctgacccgcg gcggcgcccg ccggggattt
     8761 gaactgatga tgcaaacgga tatcctgcaa aacattttac cggagatctg ccgtctcaaa
     8821 ggcgtggaac aaccgccgcg gtttcatccc gagggcgatg tctggcagca cacgttaaaa
     8881 atgcttgagt tgctgcccgc agacggagag cgggaaaaaa atatctgcct ggcgtgggga
     8941 acgctcttac acgatgtcgg taaagcgctc acccgcagcg aagacgaaaa gggcgtgcac
     9001 ttctacggcc atgtgcagca gggagaaatt attgccgaca ccatcatgca acgattaaaa
     9061 ttttcccgca tgcagagaga aaccgtcgtc aatctgattc gtcagcatat ggtattcatg
     9121 aatgtccgga aaatgcggcc cggacgtctt aaaagatttc tgcgcatgcc cgatttcgat
     9181 cttcatctgg aactgcatcg tctcgattgt ctggccagtc acggaatact ggacaattat
     9241 gagttctgcc tcgaacagat gcagatttta gcgcttgacg atttgcatcc tcctcgttta
     9301 ttaaccggaa acgatttgat cgccatgggg tttactccgg ggaaaatcat caaagatatt
     9361 cttcttgcgg tggaggaaga acagctggaa ggacgaatcc agtccgccga acaggcaata
     9421 aattatgtgc gtaacagatg gaatatagaa tcatgaaaaa ataccggctg atcatttgct
     9481 tgtttttact ttttaccctg atgctggcct gctcgggcaa ggcgcatcac cggagagttg
     9541 cgtcggcgcc cagatacaaa cctgctttaa atctgatggg ctatacgata caggcgggcg
     9601 cttttcgaaa tgttgaaaat gcagttcgct tgacggaacg gctgaaaatc aatcacgggc
     9661 tcgatgcaac ctattttctt gccgatgaca aattcttcaa agtgcgcttc ggtaatttca
     9721 gcaccaagga cctggctaaa aagagagccc tggaactgat cgacgcaaag gtcattgagg
     9781 aattttacat tgtccgtccc gaagattatt ccgtagcccg acaaaaacaa tacggaaccg
     9841 actatctcag ggaatccatt gtcacaacag cccgggattt tctcggtgtt ccctatctct
     9901 ggggcgggtc ctctcccgat gacggattcg attgcagcgg cttgaccatg acggtgtatc
     9961 agctcaacgg cctgaatctg ccccgcaact ccagaaaaca gtatgcggtc ggaaatgcca
    10021 ttgagaaaaa cgaactgcag aagggagatc tggtcttttt tgccgatcgg gggaatcatc
    10081 atgtttcgca tgtcggcata tatgtcggcg acaaccagtt cattcatgcc tcatcgcagg
    10141 gcaatgaaat tcgcattgac cacctttcgt ccgattactt caccagacaa tatgtcggag
    10201 cgagaaccta ttt
//
LOCUS       DAOSME010000072        10106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_7569_length_10106_cov_20.752363, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000072 DAOSME010000000
VERSION     DAOSME010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7569_length_10106_cov_20.752363"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PLG98_09475"
     CDS             <1..1016
                     /locus_tag="PLG98_09475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOE33531.1"
                     /translation="SVFVGCANITGMQNDIGRLAWLAGGFPESVPTNTLTMQCPSGMA
                     ATQHAARAIMCGEGDTYIASGVEDMQKVFMAMHMDFPPRLANRYNILELPMGNTAEKV
                     AEVYKVSRADMETMAYWSNKKADEATKAGKFKNEIIPMEGLKDDGTPFMVETDQWIRG
                     DVSMEQMATMKTPFKPDGVVTAATSSPLTIGAAAILLMERSKADKLGLSYSIKYEAGA
                     LGGCDPTVMGIGPIPAVQRLLARTGKKVSDIGVWELNEAFASQSLACLRELGIAQNAP
                     FDNVNIWGGALALGHPLGESGCRILVTLHSIMKTDRKDAKYGVASLCGAFGNAGATML
                     SRV"
     gene            complement(1114..2061)
                     /locus_tag="PLG98_09480"
     CDS             complement(1114..2061)
                     /locus_tag="PLG98_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE33532.1"
                     /translation="MKTLKALIPILLLCIMAVTSGCTALGWNTVPVEKLKLKYADAES
                     EIIEIDEMKIHYKDEGQGPVLILLHGVCASLHTWDGWTQRLKDRYRIIRLDIPGFGLT
                     GPAPDKSLYRKDEAVRLFEKFVDEMKLEKFYLAGNSLGGYISWNYTLKHPDRVEKLIL
                     VDSVGFPQPLPGLLAFASNPLIRPFARFTMPRFLFDNAVEQVFGDKSKVTQELKDRYF
                     DLAMREGNKGSYIDVFTEMRKLCRDENLSQGIKDIRVPTLVMWGTRDEWIPFKYFENW
                     KKELPGAKFIQYEGAGHTPMEEIPDETARDADLFLSGKM"
     gene            2283..2723
                     /locus_tag="PLG98_09485"
     CDS             2283..2723
                     /locus_tag="PLG98_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33533.1"
                     /translation="MKTYAIKYLELAEKHAEKIAARWLKDVKNNSRTPTYKSLNEAEI
                     TNQCVRFYQTFSKMFLDEKITDDSLRYFRSYAEESHTMGIPAKETLYALILMRRHIWL
                     YAEFQAIFTSGIDQRQALDTLGRTILLFDYACYEVTKEYQSLTR"
     gene            2794..3234
                     /locus_tag="PLG98_09490"
     CDS             2794..3234
                     /locus_tag="PLG98_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33534.1"
                     /translation="MVDSIKLLDITEQNAEEIAEHWAKEVHKNKRTTHYRNIKKEKLV
                     VYAVDFYRNLRKLLVPEDRLEYTREYFQKYAKKCHEIGLPLQEAIYGLVLMRRHMWLY
                     ADFQAIFIDALEHNQAIDSIMRIMLMMDYAVYEITQYYLEIAKH"
     gene            3631..5472
                     /locus_tag="PLG98_09495"
     CDS             3631..5472
                     /locus_tag="PLG98_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOE33535.1"
                     /translation="MASLWVDLRDIKFQLFEVLKIEETLLGKGRYKDFDADTCIMALD
                     AAAKFSENDIAPTYPDEVHRKPVEATFDKGKVTTPEAYKRLWKLYCENGYMAMADQPE
                     VGGQGFPAVIAAAAANMFLACNQAFVMYPGLSHGCARLMEDFFQHPLRDIVLEKMYTG
                     EWAGTMCLTEPGAGSDVGALKTTAKKNADGTYSIVGTKSFISAGDHDLTPNIIHPVLA
                     RIEGDPKGTKGISIFMVPKIRFNEKGELGEPNDVQCGNIESKMGIHGNATCTLNFGSD
                     NKCIGYLMGEERQGMPIMFHMMNEERQGVGMMGASLAMAAYLHALDYSKQRIQGQDVI
                     MMAMKVEDAPAVPIIKHPDVRRMLLKQKAISEGLRLLAYFCYFCMDNEFSAMIDGNEE
                     QKEFWHGLIEVLTPIVKSYCTEKGQDSIELAVQCYGGYGFCREYPVEQMMRDNKINLI
                     YEGTNGIQALDLLGRKLGMKRGAYFMNLLSVTKEAIEEGKKLDAFKKEAETVEKALNA
                     CAETALAFSKMIKTTPFVPLIGACDFLNCLGDALVGWLHLRMALVAIPKFFAADTGEM
                     DKAFYRGKIEGARFFINRITGLVIPHLENLKQDEQSAMNIPEESFAV"
     gene            5733..6233
                     /locus_tag="PLG98_09500"
     CDS             5733..6233
                     /locus_tag="PLG98_09500"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOE33536.1"
                     /translation="MRKRKPQTRLQSISEILFPILKKRGMISKIEEKTLQTLWPKAVG
                     PQIALQTRPDSLRNGTLFVKTASSVWVQQLHFMKEEILVKLNNLSGKPLIKEIRFVVG
                     HSPVNEKEANPVLKEKKAVLKKRDRDMIAECTEALIDQELAAVLKRIMRLEISRRRQW
                     ESRKGR"
     gene            complement(6193..6957)
                     /locus_tag="PLG98_09505"
     CDS             complement(6193..6957)
                     /locus_tag="PLG98_09505"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOE33537.1"
                     /translation="MIRTMEEETLDEILHGALRIFQKKKGYRFSIDSILLAHFVSLKK
                     RTRLMDIGCGSGIILLILTKRFPTTQCVGLEIQERLAVMARKNVQLNQLENRIDIVWG
                     DARNVRTIFQERTFDAVVFNPPYRKLHSGRINPLEERAIARHEIQGSLKDFLNAAKYL
                     LKPAGTVFTIYPAKRLVELVYLFRLHGIEPKKMKLIFSDVSSGAEFVLVEGRKGSKEE
                     LKVEPPMFIYDRPGKYSEKMSGVFSDLSGFPIDDAG"
     gene            complement(6967..8208)
                     /locus_tag="PLG98_09510"
     CDS             complement(6967..8208)
                     /locus_tag="PLG98_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOE33538.1"
                     /translation="MAIVLPFKAVRPDKKFVAQVAALPYDVMTREEGKKAVSGNPLSF
                     MHVEKSEIDVPDKTKSDDPLIYQTAKRNFTDMRVKGILIQDESPHFYVYRQKMGTRAQ
                     TGIVGVMNASEYDAGKIKKHELTRQDKEEDRIRHVDTVNAQTGPVFISYRERPGINTI
                     VDHVTFGMPEYDFTSDDGVTHTVWIISDSRHIEEIKKEFLAVDALYIADGHHRAAAAA
                     AIARTRRAKESARGFAGEYESVLAVFFPDTQLRVMDYNRAVKDLNGLTPDQFIDKIGS
                     AFIVSKDFSDRSPNQLHDFGMYLGGQWHKLTVKEGVYDKNDPVASLDAAILQEHLLYP
                     VLGIKDPRVDDRIKFIGGIRGMDELEKLVNQDGFAVAFSLYPTTMEQIINVADAGAIM
                     PPKSTWFEPKLRSGLFTHRLD"
     gene            8309..8785
                     /locus_tag="PLG98_09515"
     CDS             8309..8785
                     /locus_tag="PLG98_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE33539.1"
                     /translation="MDTLVFIHGLESTAQGTKGQFFCKNFPQMIIENYTGDFAARMRK
                     LQSLLNDKTDLIIVGSSYGGLMAVQYAIENEHRVKKLILLAPALNLAEFMPPSRVLNV
                     PVILYHGSEDDIVDPEKVKDIASRYFAALEYHLVPDDHVLHETFPSLDWKKLLTAG"
     gene            9083..9493
                     /locus_tag="PLG98_09520"
     CDS             9083..9493
                     /locus_tag="PLG98_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE33540.1"
                     /translation="MVSKKIVIRYTPDTVQQPVIYQLVKQYNLVFNILKARIFPRREG
                     VIVLELSGSKENFDHGIRFLKELGLKVEPLSKSVSQNADKCVHCGACTAFCPTGALSF
                     EKKTLKVVFDPEKCNGCELCVSACPVRAMEINLL"
     gene            complement(9546..9920)
                     /locus_tag="PLG98_09525"
     CDS             complement(9546..9920)
                     /locus_tag="PLG98_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33541.1"
                     /translation="MDVITGYISTHPAVVVIGAILIILLFLHVIFKNLLKLIFITALL
                     LIVAFGYYYVQHPAETKAKVEESFHMVESGLNEIKDKSKSFYKDGKDLYKKTKEAPGG
                     VSKLLDASEKEVEKEVGKETKK"
BASE COUNT         2750 a         2300 c         2373 g         2683 t
ORIGIN      
        1 aatccgtatt tgtcggttgc gccaacatta ccggcatgca gaatgatatc ggccgcctgg
       61 cctggctggc cggtggattt ccggaatccg ttcccactaa cacgctgacc atgcagtgtc
      121 cttccggtat ggcagccacg cagcatgccg cccgcgccat tatgtgcgga gaaggcgata
      181 cctacattgc ttccggcgtg gaagacatgc agaaagtttt catggccatg cacatggatt
      241 tcccgccccg tctggccaac cgttataaca ttcttgagct ccccatgggc aataccgcag
      301 aaaaggtcgc ggaagtttat aaagtcagcc gtgcagatat ggaaaccatg gcgtactgga
      361 gcaataagaa agccgacgaa gccaccaagg ccggaaaatt taaaaatgaa atcattccga
      421 tggaaggatt aaaagatgac ggcacaccct tcatggtcga aaccgaccag tggatccgtg
      481 gtgacgtttc gatggaacag atggcaacca tgaaaactcc tttcaaaccg gatggagttg
      541 ttacagcagc cacttcttcg cctttgacaa tcggcgccgc ggccattctt ttaatggaac
      601 gctccaaagc cgacaaactg ggactttctt acagcattaa atatgaagca ggcgctttag
      661 gcggttgcga tcccaccgtg atgggcattg gtcctattcc cgctgtccag agacttctgg
      721 cgcgcacagg caagaaagtc agcgatatcg gtgtatggga actcaatgag gcttttgcca
      781 gccagtctct ggcatgtctg agagaattgg gcattgccca gaatgctcct tttgacaatg
      841 taaacatctg gggcggcgct ctggcgctcg gtcatccgtt gggagaaagc ggatgccgca
      901 ttctggtgac cctgcacagt attatgaaga ctgaccgaaa agatgccaaa tatggtgttg
      961 cttctctttg cggcgctttc ggcaacgccg gcgccaccat gttgagcaga gtctaatacg
     1021 tgatctgcta aacgcatcca tgaaaaaggg agttcctgat atcaggaact ccctttcttt
     1081 tttttatgat gaattctatc gaccaatcaa tttttacatt tttccggaaa gaaacagatc
     1141 ggcatcgcgg gctgtttcat cggggatttc ctccatcggc gtgtgtccgg cgccttcgta
     1201 ttgtatgaat tttgctcccg gcaattcttt cttccagttt tcgaaatatt taaaaggaat
     1261 ccattcatcc ctcgttcccc acataaccag cgtcgggacg cggatgtctt ttatgccttg
     1321 ggagagattt tcatcgcggc acaattttct catttccgtg aaaacatcaa tataggatcc
     1381 tttgttgcct tctctcatgg ctagatcaaa atagcggtct ttgagttcct gggtcacttt
     1441 ggatttgtct ccgaaaacct gttcaaccgc gttgtcgaaa agaaaccgcg gcatcgtaaa
     1501 cctggcgaac ggtcggataa gcggattgct ggcaaaagca aggagtcccg gcagcggctg
     1561 gggaaatccc acggaatcaa ccagaataag cttctcaacc ctgtccggat gtttcagtgt
     1621 gtaattccag gaaatgtagc ctcccagaga gtttcccgcc agataaaact tttccagttt
     1681 catttcatca acgaattttt cgaagagcct gaccgcttcg tctttccggt acagagattt
     1741 atcgggggcc ggtccggtca gtccgaatcc cggaatatcc agccggatga tacgatagcg
     1801 atctttcagc cgctgtgtcc atccgtccca ggtgtgcagg gacgcacaga ccccatgcaa
     1861 caggatcaga acaggtccct gtccctcgtc tttataatga attttcattt catcgatttc
     1921 gatgatttct gattctgcat cggcatattt cagtttcagt ttttccaccg ggacagtgtt
     1981 ccatcccagt gccgtacagc ccgacgtgac cgccatgata cacaataaca aaatcggaat
     2041 gagtgctttg agcgttttca ttttttcggc ctttcatcaa ttgtatttaa acaacgcgag
     2101 gattttgaca agaactatag ggttttcgat tttttatgtc aataaaatat caaatcggaa
     2161 acagaactga ccgggagtcc gttcgaaaaa ctttttttat aaaaacttat tgtcaaattc
     2221 attgtcatgc tttatattaa aatcaaacat tcaaacgaat tcgagttaga aaggaggctg
     2281 ttatgaaaac ctatgccata aagtatctcg aactggcaga aaaacacgcg gaaaagatag
     2341 cggcgagatg gttgaaagac gtaaaaaaca actctaggac accgacttat aaaagtttga
     2401 atgaagcaga aatcaccaat caatgtgtca ggttttatca aaccttcagt aaaatgtttc
     2461 ttgatgaaaa aataacggat gattcattgc gctatttccg gagttatgct gaggaaagtc
     2521 ataccatggg catccccgca aaagaaacgc tgtatgctct cattctgatg agacgccata
     2581 tctggctcta tgctgaattt caggccatct ttacttcagg gattgatcaa cggcaggccc
     2641 tcgatacgct cggacgcacc attttattgt tcgattacgc ctgttatgaa gtaacgaagg
     2701 aataccagtc actgacgaga taacgataaa ataaaaaata tgtcagcaat tatttagtgc
     2761 cggacttaat ctcatagaaa ggagaatatt gttatggtcg actctataaa gttactcgat
     2821 attacggagc aaaacgccga agaaatcgcc gagcactggg cgaaggaagt tcataaaaac
     2881 aagagaacta cccattatcg aaacataaaa aaggaaaaac tggtcgttta cgcagtggat
     2941 ttttaccgga atttacgaaa attgctggtt cccgaggatc gtcttgaata tacccgggaa
     3001 tattttcaga aatatgcgaa aaaatgccat gaaatcggtc ttcccttaca ggaagcgatt
     3061 tacggacttg ttctcatgcg gcgtcacatg tggctgtacg ccgattttca ggccatcttc
     3121 atcgatgccc tggaacacaa tcaggcgatc gacagtatta tgcgaatcat gctcatgatg
     3181 gattatgcgg tttatgagat cacccagtat tatctggaaa ttgcaaagca ttgattttct
     3241 gaaagataac gtaaaaacga caaaaaaagt ccccggaatc agattatatc ggccggggat
     3301 ttttttattt gataacggaa aagtccctta atttgaaatc gttaaaattt atataagtat
     3361 ttgataatgt attgttatta tagaaatctc cggaaaaagt aaaataaaat acttaaatct
     3421 caataatatg ttgacagagt gaattttggg atgtataaca cgcaatacat gactttcagt
     3481 catttgatat aattatatta aacaatgtaa aagatcgtcc gttttttaca ttttcgcatg
     3541 tcagaaaaat tattgggaaa ggtgtctaga gtctcgtcgt tcagtcagaa cgagtacaaa
     3601 atacaataaa caaagtttaa ggaggattat atggcgagtt tgtgggttga tttacgggat
     3661 attaaattcc agttgtttga agtattaaag attgaggaaa cattgttagg caaaggtcgt
     3721 tacaaggatt ttgatgcaga tacatgcatc atggctcttg atgcggcagc aaagttttca
     3781 gagaacgata ttgctcccac ctatccggat gaggttcatc gtaaaccggt cgaggcgaca
     3841 ttcgacaaag gcaaagtaac gacgcccgaa gcttacaaga gattatggaa gctttattgc
     3901 gaaaacggtt acatggctat ggctgaccag ccggaagtcg gcggacaggg attccccgct
     3961 gtcattgccg ctgccgcagc aaatatgttt ttggcctgca atcaggcttt tgttatgtat
     4021 cccggtcttt cccacggttg tgcacgattg atggaagatt tcttccagca tccgctgcgc
     4081 gacatcgttt tggaaaaaat gtacaccggc gaatgggcgg gaacgatgtg tctgacggag
     4141 ccgggcgccg gttctgacgt cggagctctg aagacaaccg ccaaaaagaa tgctgatggc
     4201 acctactcca tcgtcggcac caagagcttc atttccgccg gcgaccatga tctgaccccg
     4261 aacatcatcc atccggttct ggccagaatc gaaggcgacc ccaagggaac aaagggcatt
     4321 tccattttca tggttcccaa aatcagattc aatgaaaagg gcgaactcgg agaacccaat
     4381 gatgtgcagt gcggcaatat cgaaagcaaa atgggtatcc acggcaacgc cacctgtaca
     4441 ctgaacttcg gttcggacaa taagtgcatc ggttatctga tgggtgaaga acgtcagggt
     4501 atgccgatca tgttccacat gatgaacgaa gaacgtcagg gcgtcggtat gatgggcgct
     4561 tctctggcaa tggccgctta tctgcacgct ctggattatt ccaaacagag aattcagggc
     4621 caggatgtca tcatgatggc catgaaggtt gaagacgcac ccgccgtgcc gatcattaaa
     4681 catccggatg tccgcagaat gctgctcaaa cagaaagcca tttccgaagg tctgcgcctt
     4741 ctggcatatt tctgctactt ctgcatggat aatgaattca gcgcaatgat cgatggtaac
     4801 gaggagcaga aagaattctg gcatggcctg atcgaagttc ttacgccgat cgtcaaatcc
     4861 tactgtacag aaaaaggcca ggacagcatc gaactggccg tgcagtgcta cggcggttac
     4921 ggtttctgcc gtgaataccc cgtcgaacag atgatgagag ataacaagat caatctgatt
     4981 tatgaaggga ccaacggtat tcaggctctt gacctgctgg gccgcaaact gggcatgaag
     5041 agaggcgctt attttatgaa cctgctgagc gttaccaagg aagctattga agaaggcaag
     5101 aaacttgatg ctttcaagaa ggaagccgag accgtagaga aagccctgaa tgcctgtgct
     5161 gaaacggcgc tggccttctc caagatgatc aaaaccacgc ctttcgttcc gctgattggc
     5221 gcctgcgatt ttctgaactg cctgggtgat gcgcttgtcg gttggctgca cctcagaatg
     5281 gccctggttg cgattcccaa gttctttgca gcagataccg gtgaaatgga taaagccttc
     5341 tatcgcggta agattgaagg tgcaagattc ttcattaacc gcatcaccgg actggtcatt
     5401 cctcatctgg aaaacctgaa acaggatgaa cagagtgcga tgaacattcc tgaagaatca
     5461 tttgctgtct aaaaaatgat tttttataaa tgaatttccg gaacggggag gcttgaagcc
     5521 tccccgtttt tttagtgaca ctcgctaagc gcgaatgtaa tgacgcgcga agcgtaagtg
     5581 gaactatccc cggagcgcag ctcagcggga tcagacactc gctaagcgcg aatataagga
     5641 aacgcgaagt aaagccgcat tatcctcccc caagcgaaag ccggacggga ttgtcccctt
     5701 gacctgtctt ttaatttatg tcagaaaaag tcatgcgcaa acgaaaaccg caaacgagat
     5761 tacaatcgat cagcgaaatc ctgttcccga ttctgaaaaa aagaggaatg atttcaaaaa
     5821 tcgaggagaa aacacttcag acactctggc cgaaagcggt gggtcctcaa atcgccttgc
     5881 aaacccggcc ggattctctg aggaatggaa cgctcttcgt caaaacagcc tcttcggtct
     5941 gggtacagca gcttcatttc atgaaagaag aaattctcgt taaattaaat aacctttccg
     6001 ggaaacccct gatcaaagaa atacgttttg tggtcggtca ctcaccggta aatgaaaaag
     6061 aggctaatcc tgttttgaag gagaagaaag cggttttaaa aaaaagagac agagacatga
     6121 ttgccgaatg cacggaagct ctgatcgacc aggaactggc cgcggtatta aaacgcatta
     6181 tgaggctgga aatcagccgg cgtcgtcaat gggaaagccg gaaaggtcgc tgaagacgcc
     6241 ggacattttc tcggaatatt ttccgggccg gtcgtaaatg aacatcggcg gttcaacctt
     6301 caactcttct ttggaacctt ttctcccttc gaccagcaca aattcggccc cggaagatac
     6361 atcagaaaaa atcagtttca tttttttggg ctcaatgccg tgtaacctga acaggtaaac
     6421 aagctccacc agtcgtttag ccggataaat cgtaaaaacc gttcccgccg gtttcaagag
     6481 atattttgcc gcgtttaaaa aatctttgag ggacccttgg atttcatgac gggcaatggc
     6541 tctttcttcc aggggattga tccggccgga atgtaatttt cggtaaggtg ggttgaatac
     6601 cactgcgtca aacgtccgtt cctggaaaat agttctgacg ttgcgggcat cgccccaaac
     6661 gatgtcaatg cggttttcca gttgattgag ttgaacgttt ttccgagcca tgaccgcaag
     6721 acgttcctga atctccaagc cgacgcattg ggtggtcgga aaacgttttg tcaggatcag
     6781 caggatgata ccgctgccgc agccgatatc catgagccgt gtccgctttt tcagagaaac
     6841 gaaatgggcc agcaggatgg aatcgatgga aaaacgatac cctttttttt tctggaaaat
     6901 acgcaaagcg ccgtgaagaa tttcatcaag ggtttcttct tccatcgttc gaatcatcaa
     6961 tctttttcaa tccagcctgt gcgtgaataa tccgctgcgc agtttgggtt cgaaccatgt
     7021 cgacttggga ggcataattg ctccggcatc ggccacattt atgatttgct ccatggtagt
     7081 gggataaaga gagaacgcca cggcaaaacc gtcctgattc accagttttt ccagctcgtc
     7141 cattcccctg atgccgccga taaatttgat ccggtcatcc acacgcgggt ctttgattcc
     7201 cagaaccgga tacaacagat gctcctgcag aatggccgcg tccagactgg cgacgggatc
     7261 gtttttgtcg taaacacctt ccttgacggt aagtttatgc cactggcctc ccagatacat
     7321 gccgaaatca tgaagctggt tcggtgatcg gtccgaaaaa tctttgctga caatgaaagc
     7381 ggaaccgatt ttatcgataa attggtcagg tgtcagaccg tttaaatcct tgacggcacg
     7441 gttataatcc ataactctca gttgcgtatc ggggaaaaac accgccagaa ccgattcgta
     7501 ttccccggcg aatcctctgg cggattcctt tgcacgtctt gtccgggcaa tggcggctgc
     7561 ggcggccgcc cggtggtgac cgtcggcgat ataaagtgcg tccacggcga gaaactcttt
     7621 ttttatttcc tcaatatgac gggagtcgga aataatccag accgtatgcg tcacgccatc
     7681 atcagaggta aaatcatatt ccggcatgcc gaaggtcacg tgatcgacaa tcgtattaat
     7741 gcccggtctt tcacgataac tgatgaatac gggaccggtc tgcgcattta cggtgtcgac
     7801 atgacggatg cggtcttctt ctttgtcttg ccgggtcaat tcatgctttt tgatttttcc
     7861 ggcatcgtat tcggaagcgt tcatcacacc gacgattccg gtttgtgccc gcgtgcccat
     7921 tttctgcctg tacacataaa aatgcggcga ttcatcctgg atcaaaatgc ccttaacacg
     7981 catatcggtg aaattccgtt tggccgtctg atagataaga gggtcatcgc ttttcgtttt
     8041 gtcggggacg tcgatttccg atttttccac atgcatgaaa ctcaaagggt tgccggaaac
     8101 cgcttttttc ccttcttccc gggtcattac atcgtacggg agagccgcca cctgagcgac
     8161 aaatttttta tccggtcgaa ccgctttaaa aggtaatact attgccataa tttgatctcc
     8221 tgacagcaaa tcttttcttg cggaatggat aacatactga cataatctat acaacataaa
     8281 aatatgccgg agaagggcag gaatacgcat ggatacgctg gtttttattc acgggctgga
     8341 gagcacggcg cagggcacca aggggcagtt tttctgtaaa aattttccgc agatgatcat
     8401 tgaaaattat acgggagatt ttgcggcgcg tatgcgaaaa cttcagagcc ttcttaacga
     8461 taaaaccgat ttgatcatcg tcggttcgag ttatggaggg ttaatggccg ttcaatacgc
     8521 catcgaaaat gaacaccggg taaaaaagct gattcttctg gcgccggcgt tgaatctcgc
     8581 tgaattcatg ccgccttccc gggttttaaa cgttcctgtc atactctacc atggctccga
     8641 agacgatatc gttgatccgg aaaaggtcaa agacattgct tcccggtatt tcgctgcgtt
     8701 ggaataccat ctggttccgg acgaccatgt tctgcatgaa acgtttccgt ctctggattg
     8761 gaagaaactg ctgacggccg gttgatgatg tcacaaagca cgcaattttg tcaaatccca
     8821 cgtcgcccct gccaactatg ttgtcgggat agttcgattt tcaaatttca ctacttcgct
     8881 cctgggataa tttccaatct cgattagtcg ggattggata attcggctga ctcttcaatc
     8941 ttcgtcccga cttgtcgggg ctcaatatta gagagtctct ctacgactag cgcttcccat
     9001 cgttagcggg tctctttaaa ttgccttgac aatccatggt tgatatctta atctttcaaa
     9061 aattttattg agtccgttta ctatggtttc taagaaaatc gtcattcgtt acacacccga
     9121 tacggttcag caaccggtga tttatcagct ggtgaagcaa tataatcttg tgttcaatat
     9181 cctgaaggcg agaatatttc cgcgccgcga aggcgttatc gttctggaat taagcggttc
     9241 caaggaaaat ttcgatcacg ggatccgttt tttaaaagag ttgggcctga aggtagaacc
     9301 gctctccaaa agcgtcagtc aaaatgccga taagtgcgtt cactgcggcg cctgcacggc
     9361 tttctgtccg acgggagccc tgtcgtttga aaaaaagacg ctgaaggtcg tctttgatcc
     9421 ggaaaaatgc aacggttgtg aactgtgtgt ttcggcctgt ccggtccgcg cgatggaaat
     9481 caatctttta taaataatat attgacagcc tccgttgaaa aaacagcggc aacggaagtg
     9541 aaattttatt ttttcgtttc tttccccacc tctttttcca cttccttttc agaggcgtcg
     9601 agtaacttgc tgacacctcc gggagcttct tttgtttttt tatacagatc ctttccgtct
     9661 ttgtagaagc ttttactttt gtctttgatt tcatttaaac cggattccac catgtgaaaa
     9721 gattcttcaa ctttagcttt tgtttccgcc ggatgctgaa cataataata accgaaagcg
     9781 acgatcaaaa gaagagccgt tataaaaatc agcttaagca gatttttaaa gattacatgc
     9841 agaaataata aaatgatcaa gatggcgcct ataacgacga cggcgggatg tgtcgatata
     9901 tagccggtga ttacatccat gataatgaac tctccttcat caaggggcat atgaaatatg
     9961 gttaagttta ccacgaagcc ttttgatttt acagtaaaaa cattaaggac ataagtaatc
    10021 agatgcagtg gatgggcatt gtgttctggc cggggggcgg gcaaattgcc tattgcgcat
    10081 attcgatttt taccttattg aagatt
//
LOCUS       DAOSME010000073        10087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_7593_length_10087_cov_21.924841, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000073 DAOSME010000000
VERSION     DAOSME010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7593_length_10087_cov_21.924841"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..568)
                     /locus_tag="PLG98_09530"
     CDS             complement(26..568)
                     /locus_tag="PLG98_09530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOE33542.1"
                     /translation="MISNISKRYARAFFDIAKEENQIEPYYKELDQFTLLISRNESLK
                     DFLANPVFEQAVKKGVVDNLIAKLKLSGMTANFIRLLVDKNRIDILEDIVVCYRQLMD
                     EALKKVRVNVKTAFTLSDELRSLISANLEKALGKTVDITVEQDPTLLGGVVIGVGDTL
                     YDGSIKNQLNNMRNLLGEAR"
     gene            complement(565..1143)
                     /gene="atpF"
                     /locus_tag="PLG98_09535"
     CDS             complement(565..1143)
                     /gene="atpF"
                     /locus_tag="PLG98_09535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOE33543.1"
                     /translation="MKKTISLITGVFVLLVAVGIAFASAEAEGGHHGADWFGLFKKAF
                     NFVVLMGLLYWLLAAKVKEFFVGRRAEIKENLEKAVERKAEAEKKYREYSEKIDKAST
                     EIDGIIEMIKAQGVTEKQKIIEDAERTAKKMKEDAHARIEQEMKKATEQLKSQAVELS
                     VQMAEEILKKSITQDDHAAMVKEYVNKVVIKH"
     gene            complement(1140..1574)
                     /locus_tag="PLG98_09540"
     CDS             complement(1140..1574)
                     /locus_tag="PLG98_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOE33544.1"
                     /translation="MVTVIPDYTLLVQGVLFLTLVFLLNILLYKPILSIIDRRKKQLE
                     ESENEIRLFDENAEKKVAEYEEKLKQAKLKASEAKKEVIQEGANQAKNIIDAVRNEIP
                     VMAREFQQKMDKEVEKAKAVLDGNSRQLSLEIAQKILGRPVQ"
     gene            complement(1913..2290)
                     /locus_tag="PLG98_09545"
     CDS             complement(1913..2290)
                     /locus_tag="PLG98_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOE33545.1"
                     /translation="MWEKKKDAEEIKAFLGQGAEFSGKLIFNGSVRIDGKFEGEIFGQ
                     GSLVVGQGATVKANIDTKSVYISGDVQGNIDVKEKINIHSTGKFSGDVRTPVFVMEAG
                     AIFNGMSHMDDVAQQKIEIPKAD"
     gene            complement(2363..3478)
                     /gene="rodA"
                     /locus_tag="PLG98_09550"
     CDS             complement(2363..3478)
                     /gene="rodA"
                     /locus_tag="PLG98_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOE33546.1"
                     /translation="MIFLKFDRRIFHYFDWILLSFVLGICVMGVLNIYSTGFSAAGGQ
                     SPFYLKQLQWIVVGLLFMTFVFFMDYRVIVEYAYIIYGFSIFLLVLVLIVGSSSHGAQ
                     RWLGGGFFAIQPSELMKVVIILTLARYFDNHKSSEPYTLKELFIPLLIVAVPFVLILK
                     QPDLGTALMLMVIFASIVFFMGVDKKSLIFVFAAGLVLMPAGWFFLKDYQRERLVTFL
                     NPERDPLGSGYHIIQSKIAIGSGEIFGKGFLSGSQTQLKFLPEQQTDFVFSVFAEEWG
                     FIGSMLLIGLFVVIIFWGLKIALQARDLLGTIIAFGVSALIAWEVFINIGMVLGILPV
                     VGIPLPFFSYGGSAMLSLMAAIGLLNNVSARRFILQK"
     gene            complement(3475..5364)
                     /gene="mrdA"
                     /locus_tag="PLG98_09555"
     CDS             complement(3475..5364)
                     /gene="mrdA"
                     /locus_tag="PLG98_09555"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE33547.1"
                     /translation="MLNGHEPIEFKGKIRIITGIFLLAVCILIGRLGYLQIIKGEEFK
                     KKSENNSVRFRFIKPLRGLIMDRNGNVLVDNKPSFDVIYIPNKNKANELSVEKLKSLY
                     ESQSMVFPFDQAVLKNAKPYLPVKLDKNVSMEKIALVETNALNLPGIYIDVSPIRLYL
                     DGEILAPIIGYTGEISREEMEKSEGKYAYGDISGKHGLERFFDEYIRGRRGAELVEVN
                     VYGREIKNLGRIDPVSGYNMTLTIDADLQRAAWQAIEGKAGSVVALDPRDGAILAMVS
                     SPSFDPNLFNEGISSELWKQLQSNPFAPLSNKAISGQYPPGSTYKLIVAAAALEEGII
                     SPETKIFCNGFFTLGNRTYRCWKKGGHGHVNLHQAMVGSCDVYFYTVGKMLGVDKIAE
                     YAKRFGLGDLAGVDLPNEKKGLVPTKAWKLKKKKEAWQMGETISISIGQGFNLVTTLQ
                     LANAYAAFANGGTLWRPYLVKKIDAPDGKTYKDFPPVKKGELKLSNTTMEIINDALKA
                     VVNEPGGTGQNAKLAQIEVCGKTGTSQVLGLPENEKARRRKVLSAFHKDHALFASYAP
                     SKSPEIVVAVILENAGGGGAVAAPVARRVLSAYFEGKKKEKSAGKSEKPGESDNRRNL
                     DGDDR"
     assembly_gap    5477..5576
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5577..6015)
                     /locus_tag="PLG98_09560"
     CDS             complement(<5577..6015)
                     /locus_tag="PLG98_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33548.1"
                     /translation="MFHYLLLLILSLLLIVLQSTVTDIVFSNSFVFEVSLIIIIYAGF
                     RFEMIKGLLLAFYLGFILDLIGGSVPGVLALAYIMIFLCSFFISDLLDTGKNHVIVLF
                     SFICVFLKETMLQTFYFLVFDIRMRPDIFSLILIQSFVVGLVAP"
     gene            complement(6018..6845)
                     /gene="mreC"
                     /locus_tag="PLG98_09565"
     CDS             complement(6018..6845)
                     /gene="mreC"
                     /locus_tag="PLG98_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOE33549.1"
                     /translation="MVFLKKYKVIIIIAVLMVLVLVVISYHLKFGDDKSFLRKVALEI
                     SSPVYKIINGSIQGIKDAWLHYFLLVGVQEENKKLKKKMDELQAVIVSYQEGYLEAQR
                     LKKLLAIPDYHRYPFIAARVIGREQATLSRTVLINKGASDGLKNGMPVVAYPGLIGRL
                     TNVSRNVSRVLLFIDENSNVDAIVQRDRIQGIISGAGPRGMILKYIPKTQDVRKGDII
                     VSSGMGGVFPKGWLIGQVINVDRQNDGLFLKIDVAPFTDFSKLEELLILSSAETAVE"
     gene            complement(7048..8085)
                     /locus_tag="PLG98_09570"
     CDS             complement(7048..8085)
                     /locus_tag="PLG98_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOE33550.1"
                     /translation="MFFDYVLGKFSNDLAIDLGTANTIVYVKDKGIVLNEPSVVAVHQ
                     DARGMKKVLAVGNEAKNMLGRTPGNIVAIRPMRDGVIADFDITEAMLKHFILSVHNRK
                     SLVRPRIIISVPSGITQVERRAVRETVESAGAREIYLIEEPMAAAIGAGLPVAEPTSS
                     MVVDIGGGTTEVAVISLAGIVYSKSVRVAGDKIDEAIVQYMKRKHNLLIGERTAEIIK
                     MEIGCAYPFDEIKVGSIKGRDLISGIPKITETNSEEIREAINEQVTFIVDAIKDTLEN
                     SPPELAGDIVDRGIVLTGGGALLANIDILIREETGLPITITDDPLSAVARGAGMALEQ
                     INVLKDIALEV"
     gene            8270..9865
                     /locus_tag="PLG98_09575"
     CDS             8270..9865
                     /locus_tag="PLG98_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOE33551.1"
                     /translation="MLKFFRKHARGWFMIAVIAIIIVVFVLYFGASRGGEHAQVVATV
                     DKKAITVNEVLNEYDKLLEVARLNLKENLTPELIKKMDLKTKAYNDILNREVIIAKAA
                     DLKIQVSDDELRNSILSMPALQTNGMFDERKYHQILRFNRLSAEDFETLQRADLTARK
                     IEFLVREGVKISDKEIYDLYALQNRKINVDFLRLAASDVKHKISPVESELEDYLKRNG
                     NLFRVPEQTKIKYLFFGADTFPADISDADIQDYYSTYQNKYKAKDGKPLPLAGVRNDI
                     INELKRAKGMQTAYTEARKAREEIYQDDNMDAYARKHNLAVRDSDFFMITKPPQDFAS
                     VKNFADALLNMQKGELSKILSTENGYYLLQVVDKKASYIPKLDTIANEVRQRFIETET
                     QLLAEKEAKSILESLKAGESMEKVASAKGLKIGQTGFFQPGNTIPGVGESPEAAEILM
                     TLSAANPYAEKPLFINNVYFILKFRETTRIDDNAFTAEKEIYRKILTAMKQEEAMQTW
                     LEGNKADMIKEKRIKIKKKAEDL"
BASE COUNT         2806 a         2484 c         2048 g         2649 t
ORIGIN      
        1 cttccgcttt aattgtttcc atgtcctatc ttgcctcccc taagagattc ctcatattgt
       61 tcaactgatt ttttatactg ccatcgtaaa gtgtatcgcc aacgccaatg accacgccac
      121 ccaaaagggt ggggtcctgt tccaccgtta tatcaactgt tttacccagc gctttttcca
      181 ggtttgcact gattaaagaa cgcaactcat ctgaaagagt gaaagcggtt tttacattca
      241 cccgcacctt cttcagcgct tcgtccatta actggcgata acaaacgacg atatcctcta
      301 aaatatcgat tcgattcttg tccaccagca gcctgataaa gttggccgtc atgccggaca
      361 acttcaactt ggcaatgaga ttgtccacaa cacctttttt aactgcctgt tcaaagacag
      421 gatttgccag gaaatctttt aaagattcat tccgagaaat gagtaatgtg aactgatcga
      481 gttccttgta ataaggctca atctggtttt cctctttcgc aatgtcaaaa aaggcacgcg
      541 catatctttt ggaaatattg ctgatcaatg cttgattacc accttattta cgtattcttt
      601 caccatggct gcgtgatcat cctgtgtaat gcttttcttc agaatttctt ccgccatttg
      661 cacagaaagt tcaacagcct gggatttcag ctgttctgta gcttttttca tttcctgctc
      721 gatgcgggcg tgagcatctt ctttcatctt tttggctgtt ctctcggcat cctcgataat
      781 cttctgtttc tccgtaacgc cctgcgcctt gatcatctca atgataccgt ctatttctgt
      841 tgacgccttg tcaatttttt ccgaatattc acgatacttt ttctcggctt cggcttttct
      901 ctccaccgcc ttctccagat tttccttaat ctccgcccgg cgaccgacga aaaattcttt
      961 caccttggca gccagaagcc aatagagaag acccattaaa acgacaaagt tgaaagcctt
     1021 cttgaacaaa ccgaaccagt ctgcaccgtg gtgcccccct tcagcctcag cggaagcaaa
     1081 tgcaataccc acagcaacca gcaatacaaa cacacccgtt atcagagata ttgttttttt
     1141 cattgtaccg gcctccccaa tattttctgg gcgatctcaa gggataactg acgggaattg
     1201 ccgtccagaa ccgcttttgc cttttccact tccttatcca tcttctgctg gaactcgcgg
     1261 gccattaccg gaatttcatt gcgcacggca tcaataatat tttttgcctg attcgcacct
     1321 tcctgtatga cctctttttt agcctcggac gctttgagct tggcctgctt taatttttcc
     1381 tcatactctg caactttttt ctcggcgttt tcatcaaaaa gcctgatctc gttctctgat
     1441 tcttcaagct gttttttgcg ccggtcaata atggaaagga tgggtttgta aagaaggatg
     1501 ttgagtaaaa aaactaaggt caggaaaagg acgccttgaa ctaacaacgt gtaatcggga
     1561 atgactgtaa ccatttttta cctcatgctt atcttttgca ctttgtaaaa accgctggaa
     1621 gattttcctt tatggacggt ttttgatggc atgcctaagc aagtgcatac agtttttatt
     1681 cagttgtgtt ttgaatattt ttacctaaaa aggatggaat ctaatttcca gatactcaaa
     1741 acctgcgagt tactaatcac atcattatgg gcttgtcaag tattttctga ccatcagtct
     1801 gtttttctgc aataattaat aatatcaaag acttggggaa ttaaaatgct gtcaggctgt
     1861 tttttatgcg tttgacgtca agacccaaat aatactgatg gtttgcacaa aatcaatccg
     1921 ccttgggtat ttcaattttc tgctgcgcaa cgtcatccat atgggacatc ccattaaaga
     1981 tggcgcccgc ttccataaca aacacagggg ttcgcacatc tccggaaaat ttccctgttg
     2041 agtgaatatt gattttttct tttacatcga tgttgccctg cacgtcgccg ctgatataaa
     2101 cacttttggt atcgatgttg gccttgacgg ttgcgccctg accgacaacc agtgatccct
     2161 gtccgaaaat ttctccttcg aattttccgt ctatgcgaac ggaaccgtta aaaataagtt
     2221 ttcctgaaaa ctcggctccc tgaccgagga aggcttttat ttcttcagca tctttctttt
     2281 tttcccacac atcgtccccc ttttgatact tatgttaatc gattcggctc aaaatgattt
     2341 tctctttata ctcgatgact ccttactttt gcaggataaa ccggcgcgcg ctgacattgt
     2401 tcagcaatcc gatggcggcc atcagcgaaa gcatggcgga tcccccataa ctgaaaaaag
     2461 gaaggggaat accgacaacg ggcaggatgc ccagcaccat tcctatattg ataaagactt
     2521 cccacgcaat cagagcgctg acaccgaacg ctataattgt gcctaataaa tctctggctt
     2581 gcaaggcaat ttttaatccc cagaatatga tcaccacaaa caggcctatc agcaacatgg
     2641 aaccgatgaa tccccactcc tcggcaaaaa cggaaaaaac aaaatcggtc tgctgctcgg
     2701 gaaggaactt caactgggtt tgggaaccgc ttaaaaaacc cttgccgaaa atttctccgg
     2761 agccaatggc aattttcgat tggatgatat gataaccgga gcccagtgga tctctttccg
     2821 gattgagaaa cgtcacaagc ctttcccgtt gatagtcctt gaggaaaaac cagccagccg
     2881 gcattaagac caaaccggcg gcaaaaacaa aaatcagaga tttcttatcc acacccatga
     2941 aaaacactat ggaagcaaag atgaccatca gcatcagagc cgttcccaga tccggctgtt
     3001 ttaaaatcag cacgaaagga acagcgacaa tcaagagcgg aatgaaaagc tcctttaacg
     3061 tgtaaggctc gctggatttg tggttgtcga aataacgcgc cagggtaagg atgatgacca
     3121 ctttcatcaa ctcggaaggc tgaatggcaa agaaaccgcc gcctagccac ctctgggcgc
     3181 catgcgacga cgaaccgacg attaaaacca gaaccaggag aaaaatggaa aaaccgtaaa
     3241 tgatgtaggc atactcgaca attacccggt aatccatgaa aaacacaaac gtcatgaaaa
     3301 gcaatcccac tacaatccat tgcagttgct tcaggtaaaa aggcgactgg cctcctgctg
     3361 cactgaaacc ggtggagtag atattcaaaa ctcccatcac gcagatgccg agcacaaaag
     3421 acaacagaat ccagtcaaaa taatgaaaaa tacggcgatc gaatttaaga aaaatcatct
     3481 gtcgtcccca tcaagatttc tgcggttgtc agattcgccg ggcttttcac tcttcccggc
     3541 gcttttttct tttttcttcc cttcaaaata agcggataat actctgcgcg cgaccggtgc
     3601 ggcaacggct ccgccgcctc cggcattttc gaggatgacg gccacaacga tttccggact
     3661 tttcgacggc gcataactgg cgaacagcgc atggtctttg tgaaaggcgc tcaggacctt
     3721 gcggcgtcgc gccttttcat tttccggcaa tcccaacacc tgggatgtcc ctgttttacc
     3781 gcatacctct atctgtgcca gtttcgcatt ctgccccgtg cctccgggct cattgaccac
     3841 ggcctttaaa gcatcattta taatttccat cgtggtgttg ctcagtttca actccccctt
     3901 tttcacgggc gggaaatcct tataggtttt accgtcggga gcatctattt tcttgaccag
     3961 atacggacgc cagagggtgc ctccattggc aaaagcagcg taggcattgg ccagttgtaa
     4021 agttgtaacc agattaaacc cctgtccgat ggatatcgat atggtttcgc ccatctgcca
     4081 ggcctctttt ttctttttca acttccatgc cttggtcggc accagtcctt tcttctcatt
     4141 aggtaaatcg acacccgcca gatctcccag accgaaccgt tttgcgtatt cggctatttt
     4201 gtccacgccc agcattttgc ccacggtata aaagtaaaca tcacatgatc cgaccatggc
     4261 ctgatgaaga ttgacatgac cgtgaccgcc ctttttccag cagcgatatg ttctgtttcc
     4321 cagagtaaaa aagccgttgc agaaaatttt cgtctcgggc gagataatac cttcttccaa
     4381 agcagctgcg gcaacaatta atttataggt tgatcccgga gggtattgtc cggagatcgc
     4441 tttattggac aacggggcaa acggattgct ctgcaactgt ttccagagtt cgctggaaat
     4501 accttcatta aataaatttg gatcaaacga aggggaactc accatggcca ggatggcgcc
     4561 atcgcgcgga tccagtgcga caacggaacc cgctttccct tcaatggcct gccatgccgc
     4621 tctttgcaag tccgcatcga ttgtcagagt catgttatag ccggagacgg gatcgatgcg
     4681 gcccaggttt tttatttctc tcccgtaaac attgacttca accagttctg caccgcggcg
     4741 gccgcgaata tattcgtcga aaaatcgttc caaaccatgt tttccggata tatcgccgta
     4801 agcatacttg ccctcgcttt tttccatttc ttccctgctg atttcaccgg tgtaaccgat
     4861 gatcggcgcc aggatttctc cgtcaagata caaacgaatc ggcgagacat caatgtaaat
     4921 cccgggcaga tttaacgcgt tggtttccac cagggctatt ttttccatac tgacgttctt
     4981 gtccaacttt acaggcaaat acggttttgc gtttttcaga acggcttgat caaacgggaa
     5041 aaccatcgat tgactttcat aaagcgattt taatttctcc actgacaatt cattggcctt
     5101 gtttttgttg ggaatataaa tcacgtcgaa agacggtttg ttgtcgacca gaacattgcc
     5161 gttacggtcc atgatcagac cccgtagcgg cttaataaac cgaaaacgca cggagttatt
     5221 ttccgatttc tttttgaatt cttctccttt gataatctgc aaatatccca agcgaccgat
     5281 caggatacag acggcaagca gaaatatccc cgttatgatc ctgattttcc ccttgaattc
     5341 aatcggttcg tgtccgttaa gcatatcatg ccttcttttc gtagaggaat attccagttc
     5401 tgtcgataag ataaaacacc gcgggagcga ccagtccgac aacaaatgac tgtatcagaa
     5461 tcagagaaaa tatatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg
     5581 agcgaccagt ccgacaacaa atgactgtat cagaatcaga gaaaatatat cagggcgcat
     5641 cctgatatca aagaccagaa aataaaaggt ctggagcatc gtctctttca ggaaaacaca
     5701 aatgaaactg aataaaacaa tgacatgatt tttcccggta tcaagcaaat cggatatgaa
     5761 aaatgaacac aggaaaatca tgatatacgc caaagcaagc accccgggaa ccgaaccgcc
     5821 gattaaatcc agaataaatc cgaggtaaaa ggccagcaac aaccctttga tcatttcaaa
     5881 acggaatcct gcgtaaatga taatgatcag agatacttca aaaacaaaag aatttgaaaa
     5941 cacaatatcg gtaacagtgg actgcagcac gataagcagt agggataaaa tcagaagcag
     6001 caaataatga aacatgatta ttcaacggcc gtttcagcgg aagataaaat taataattcc
     6061 tccagcttgg aaaaatccgt aaacggagca acatcaattt ttaaaaacag gccatcattc
     6121 tgccggtcca cattgatgac ctggccgatc aaccatcctt tgggaaaaac acctcccatg
     6181 cctgaagaaa caatgatatc ccctttccgg acatcctgtg tcttgggtat atactttaat
     6241 atcattcccc ggggaccggc gccactgata attccctgaa tgcgatcccg ctgcacgata
     6301 gcgtcaacat tgctgttttc gtcgataaac aaaagaaccc gggagacatt ccgggagaca
     6361 tttgtaagac ggccgatcag ccctggatag gcaacgaccg gcataccgtt tttcaaaccg
     6421 tcggatgcac ctttattgat cagaacagtt ctggacagcg tggcctgctc tctgccgatc
     6481 accctggcgg caatgaaagg atatcggtga taatcgggaa ttgctaaaag cttcttcagg
     6541 cgctgcgctt ccagataacc ttcctgatag gaaacaataa ccgcctggag ttcatccatc
     6601 ttttttttta attttttatt ttcttcctga acgccgacta gcaagaaata gtgaagccat
     6661 gcatccttga tgccctgaat ggaaccgttg atgattttat acaccggaga agatatttcc
     6721 agcgcaactt ttcttaaaaa acttttgtca tcgccgaatt tcagatggta tgaaatcaca
     6781 accaggacta aaaccataag gacggcaatg ataatgatga ccttgtattt ttttaaaaaa
     6841 accattttta acctgacggg gaaatgatta caaaacgcat tttatgtcaa tatgccggga
     6901 cgcaagactg acaaaatttc ttcctttcgc aaaagatcat gaaagaaatt tatcttgctg
     6961 ttttttcact tcatcgtaaa aatcactcaa aaccgccggg aaacgattcc gtaataaaaa
     7021 actgcacaca gattaaaggt ctgattttta aacctcaagg gcgatgtcct tgagaacatt
     7081 tatttgttca agggccatgc ccgcacctct ggccacagcc gacagcggat catccgtaat
     7141 ggtgatgggc agtcccgttt cttcccggat gaggatgtca atattggcga gcaaagctcc
     7201 gcctccggtc aggacgatac cgcggtcaac aatatcaccg gcaagctccg gaggcgaatt
     7261 ctccaacgta tctttaatgg catcgacaat gaaggtcacc tgttcgttga tggcttcccg
     7321 tatttcttcc gaattcgttt ccgtaatctt gggaatgcct gaaatcagat cccgcccttt
     7381 gatggacccg actttaatct catcaaacgg atacgcacag ccgatttcca ttttaatgat
     7441 ttccgccgtt cgttcaccga tcagcagatt atgttttcgt ttcatatatt gaacaatcgc
     7501 ctcgtcgatt ttatccccgg cgactctcac ggatttcgaa taaacgattc cggccaggga
     7561 aatcaccgcg acttccgtcg tcccgccgcc gatgtcgaca accatcgaac tggtcggttc
     7621 ggcaacaggc aaccccgctc cgatagccgc agccatcggc tcttcaatca gataaatttc
     7681 ccgggcccct gcggattcga ccgtctcgcg caccgcgcgc ctctccacct gcgtgatccc
     7741 cgaaggcacg gaaataatga tccggggcct cacgagtgat ttgcggttgt ggacgctcag
     7801 aataaaatgt ttcagcatcg cttccgtaat gtcaaaatcg gcaatgacgc cgtcgcgcat
     7861 cggtctgatg gccacaatat ttccgggtgt ccgccccagc atattttttg cttcatttcc
     7921 caccgccagc acttttttca tcccccgggc atcctgatga acagccacca cggagggttc
     7981 attcaagaca attcccttat ccttgacgta tacaatcgta ttcgccgtac caagatcaat
     8041 cgccagatcg ttggaaaatt ttccaagcac ataatcaaaa aacattgggc ctcctgtaat
     8101 ttacaaatta atataatgac tttcaggctt taaatttttt tcatacagta tttcacggac
     8161 ctaatcaact catttttaaa ttatatttgc attttgtaaa tgactataat aataagacaa
     8221 aaattaattt ccgaatgaat aaatcctgta tcgttttgag gtgattttta tgttgaaatt
     8281 ttttcgcaaa cacgcccgcg gctggttcat gatcgctgtg atcgcaatca ttatcgtggt
     8341 tttcgttctt tacttcggcg ccagcagagg aggcgaacac gcccaggtgg ttgccacagt
     8401 ggacaaaaaa gccatcacgg tcaatgaagt actcaacgaa tacgacaaac tcctggaagt
     8461 ggccaggctc aacctgaaag agaatctgac accggaactg atcaaaaaaa tggatctaaa
     8521 aacgaaagcc tataatgaca tcctgaacag agaagtgatt atcgccaaag cggctgattt
     8581 aaagattcag gtttccgatg atgaactgcg caattccatc ctgtccatgc cggccctgca
     8641 gacaaacggc atgtttgacg aacgcaaata ccatcagatt ctacgcttca acaggctatc
     8701 cgccgaagat tttgaaacgc tgcagagagc cgatttgacg gcccgcaaga ttgaatttct
     8761 tgtgcgggaa ggcgtaaaaa tttccgataa agaaatttac gacttatacg ctctgcagaa
     8821 ccggaaaata aacgtggact ttttgcgcct ggctgccagt gatgtaaagc ataaaatttc
     8881 gccggttgaa tcggaactgg aagattatct gaaacgaaac ggcaatcttt ttcgcgtccc
     8941 cgaacagaca aaaatcaaat acctgttttt cggcgctgac acttttccgg ccgatatcag
     9001 cgatgccgat attcaggatt attattcaac gtatcaaaat aagtacaaag cgaaagacgg
     9061 caagccattg ccactggccg gggtccggaa tgatatcatc aacgagttga agagagcaaa
     9121 gggcatgcaa actgcctata ccgaagccag aaaagcccgc gaggaaatct atcaggacga
     9181 caatatggac gcctacgccc ggaaacacaa tctggcagta cgtgattccg attttttcat
     9241 gatcaccaaa ccgccgcagg acttcgcttc cgtcaagaat ttcgccgatg cgctgctgaa
     9301 catgcaaaaa ggcgaactga gcaaaattct ctcgaccgaa aacggctatt atctcttgca
     9361 ggtcgtcgac aagaaagctt cctacattcc gaaactcgac acgatcgcca atgaggtccg
     9421 gcaacgattt atcgaaacag aaacacagct tcttgccgaa aaagaagcta aatcgattct
     9481 ggaaagcctg aaagccggcg aaagcatgga aaaagtggcg tccgccaaag gattaaagat
     9541 cggtcagacc ggattttttc agcccggcaa taccatcccc ggcgtgggag aaagtcctga
     9601 agccgccgag attctgatga ccctctccgc ggccaaccct tacgccgaaa aacctctttt
     9661 catcaataat gtctacttta tattaaaatt cagggagaca accagaatcg acgataatgc
     9721 tttcactgcg gaaaaagaaa tttacagaaa aatattaacg gcgatgaaac aggaagaagc
     9781 catgcagacc tggctggaag gaaacaaagc ggacatgatc aaggaaaagc ggatcaagat
     9841 taaaaagaag gcggaagatt tataattaca taaaatcacg tgtcgttcgg cgcacaatac
     9901 cgtccgggac acgtgaacgc ggccatacta tactttctct ttaatgtgag cgttttgtgg
     9961 gccagcgcaa ccatcaaaca tttcgctttt gaaaattgca actctttaaa aaacagaagg
    10021 gaggggacaa acacccctcc ctttaaattt tggtggagct gaggaggatc gaactcctga
    10081 cctcttg
//
LOCUS       DAOSME010000074         9980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_7689_length_9980_cov_14.585088, whole genome shotgun sequence.
ACCESSION   DAOSME010000074 DAOSME010000000
VERSION     DAOSME010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_9980_cov_14.585088"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..399)
                     /locus_tag="PLG98_09580"
     CDS             complement(118..399)
                     /locus_tag="PLG98_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE33552.1"
                     /translation="MPNAPKIIPISDLRQNASDVVKSISSSREPVFITQRGRAAAVMV
                     SMEVYKNAQHEMDILHLLARGEKEIAAGTGYELDEVLREADLYLETAKL"
     gene            complement(562..894)
                     /locus_tag="PLG98_09585"
     CDS             complement(562..894)
                     /locus_tag="PLG98_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOE33553.1"
                     /translation="MAKFVKGDVVVIPFPFSDLSQSKRRPALVLAALQGDDLILCQIT
                     SKSIKDNYAIPVDENDFESGSLNQESNIRPNRLFTADNHIILYRIGNIKIIKLNQVID
                     KVVEIIKE"
     gene            complement(879..1073)
                     /locus_tag="PLG98_09590"
     CDS             complement(879..1073)
                     /locus_tag="PLG98_09590"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HOE33554.1"
                     /translation="MNTKEELISEIEKTPEPFLSEVLDFVHFLKAKARREKLDTAVMS
                     ESSLAKDWLKQEEDGAWQSL"
     gene            complement(1326..1589)
                     /locus_tag="PLG98_09595"
     CDS             complement(1326..1589)
                     /locus_tag="PLG98_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOE33555.1"
                     /translation="MSWKLVFTKQAQKDAKHISQSGLKPQASRLLDILKEDPFQNPPH
                     YEKLIGDLSGAYSRRINIQHRLIYQVLKDIHTVKIIRMWAHYE"
     gene            complement(1586..1828)
                     /locus_tag="PLG98_09600"
     CDS             complement(1586..1828)
                     /locus_tag="PLG98_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE33556.1"
                     /translation="MTTFTASEARKRLYNLVDEVKDSHVPVQIVGKRNSAVLVSEEDW
                     RAIEETLYLASIPGMRESIKKGLKTPVEKCKEEPGW"
     gene            complement(1951..2325)
                     /locus_tag="PLG98_09605"
     CDS             complement(1951..2325)
                     /locus_tag="PLG98_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE33557.1"
                     /translation="MNIVDSSGWLAYFADEPNAKHFLPPLSDSDLLVVPVITIYEVFK
                     VILRESSENEALQAVVAMQKGKVVDLSTQLAIAASRLSLEHQLPMADSIILATAQEFK
                     AIIWTQDSDFKNISKVKYFPKK"
     gene            complement(2322..2528)
                     /locus_tag="PLG98_09610"
     CDS             complement(2322..2528)
                     /locus_tag="PLG98_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOE33558.1"
                     /translation="MQSVTVSPKYQVVIPKTVREALNLHPGQKMQIVEYAGRIELIPE
                     RDIKELRGFLKGINTEFKREEDRV"
     gene            complement(3160..3960)
                     /gene="istB"
                     /locus_tag="PLG98_09615"
     CDS             complement(3160..3960)
                     /gene="istB"
                     /locus_tag="PLG98_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOE33559.1"
                     /translation="MMNPMPELIPMLKQLRLSGILDSIESRNRQAIENQFSYMDFLAT
                     IVHDEIARRSQKRLASALRRANFRNQKTLEEFDFTFNPNINRALIMELASCRFMAEKV
                     SVLIVGPCGTGKSHLAQALGHSAIRAGHDVLFTTASRMLAQINAARATNSYERQLAKL
                     VGIDLLIIDDFGLKPLTGIQDEDFHEVISERYERKSTIVTSNLDITEWPDAFPNRILG
                     AATIDRLRHGAYKIVLDGKSYRAHPRSETQNTSCQKTEKQTKKGGQIA"
     gene            complement(3975..5465)
                     /gene="istA"
                     /locus_tag="PLG98_09620"
     CDS             complement(3975..5465)
                     /gene="istA"
                     /locus_tag="PLG98_09620"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOE33560.1"
                     /translation="MGESDRTIARARLMGRIKCGQVRAIAGEQGWLDRGLPLPDDEVL
                     GAAFEQKKEHDNNPTHQSLSGAYEEQIRKWVEGNVCMTTIHQTLADQFGFTGSYSSVR
                     RLVQRLKLHQPEATCILDFAPGEAVQVDFGKGPDITDAFTGQTFKTWIFVMTLCFSRH
                     LYAEIVTDQKVGTWLSCHRRAFEFFNGVPAKVIIDNPKCAITKSCFRDPEVQRSYGEL
                     AEGYGFLISPCPPRDPQKKGRVESGVKYVKNRFVPLRQFRSLADANGQLRQWILETAG
                     NRIHGTTRQKPLSLFAEAERHLLKPLPDVPVEIAVWCQVKLHANCHVQFEKAYYSAPF
                     TLIHQTMWIKATDSAVKIYHDLKLVAVHPRLRKPGARSTVDEHLPPEAIAYKLQDPRW
                     CLRQAEAIGPACHQMIRTLFADRVLDNLRAAQGVVGLGKKYGPVRLESACKRALHFEN
                     PRYKTVKTILEKGLDQTSVPGDTVPLSSVYTGSGRFLRQPGVMRYH"
     gene            complement(5960..6478)
                     /locus_tag="PLG98_09625"
     CDS             complement(5960..6478)
                     /locus_tag="PLG98_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33561.1"
                     /translation="MAMDEWIKEAVDSFVAEEGDWLCCSLKKVKFVSDNRQATALYGF
                     EWLIQTAYSHWLLHQRDVTDIKIGLKSNNGKKYDLDFQVSNQRLIIEIKTIAPGGISY
                     VKSDVNKVFPSNCTPYFLVFSYPHDSEDIPRLDGTTAIGSGVAPQGFRYYLYKKSEQA
                     APPDRVPAACSR"
     gene            complement(6481..6846)
                     /locus_tag="PLG98_09630"
     CDS             complement(6481..6846)
                     /locus_tag="PLG98_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33562.1"
                     /translation="MSDTINSEKELGNALKEEKDTIIIEGDLVKKVVRIRATGKVAWA
                     IAAGAIAVAIIIVIGTGGTGTPGAALVGGAAVGILGLPAALAAISIGVAAGGVAALNK
                     LRQYKEVERSAGRLVLKRK"
     gene            7020..8336
                     /locus_tag="PLG98_09635"
     CDS             7020..8336
                     /locus_tag="PLG98_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOE33563.1"
                     /translation="MENLSDCRKNDVLPEFQAFLLDKKLAQEKNVFFYALWASKFLNY
                     SNKKQIPPDQYQENAVIEFIETLKSNSAVSDWQIRQAGDAIRLYYFHYRGFKPSNLPA
                     GQSAGFAQELLTEAKRLIRLKHYSYSTEKTYLQWIKRFLDYARQAGGKETVAEVGAVD
                     CKNFLSHLALQERVSASTQNQAFNALLFLFRHVLCKDMGNMAETVRAKRGQKLPVVFS
                     VEEVKRLLSCLTGRDLLVAGLLYGSGMRLMELARLRVKDVDMDFNTLTVRSGKGDKDR
                     TTILPATVKDQLKNHLAEVKKLHENDLTRGYGEVHLPDALSRKYPNAGKEWAWQYVFP
                     ANNLSVDPRSGAVRRHHISDTAIQGMIKKAIRKAGIPKFATVHTLRHSFATHLLMNGV
                     NIREVQELLGHKNVETTMIYTHVLRDMSHAPQSPLDTLLAGAKNPS"
     gene            8443..9069
                     /gene="hisH"
                     /locus_tag="PLG98_09640"
     CDS             8443..9069
                     /gene="hisH"
                     /locus_tag="PLG98_09640"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOE33564.1"
                     /translation="MIAIIEYNAGNITSVARALHSIGRDFIITDDTKMLDAASHAIFP
                     GVGAAGEAMAYLRDKKLDMWLKQCFRSGKPILGICLGTQIILEASEENNTQCIGLVGG
                     TTRRFPESLTADGRALKIPHMGWNSVAFRRPHPVFACVPEEAEFYFVHSFYPSPADPS
                     AVLGTTEYGMTFCSVLAVNNLVAVQFHPEKSGRPGLQILKNFCAWGGK"
     gene            9066..9845
                     /gene="hisF"
                     /locus_tag="PLG98_09645"
     CDS             9066..9845
                     /gene="hisF"
                     /locus_tag="PLG98_09645"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOE33565.1"
                     /translation="MISKRIIPCLDVRDGKLTKGIKFKGNIDIGDPVEAAREYYEQGA
                     DEIVFYDITASSDKRDIMIDVVRRVAETIFIPFSVGGGIRSLDDMRRVIGAGAEKVSV
                     NSAAVREPAVISQGARAFGSQCVVLGMDVKKTAVSKKTPSGYEIVIEGGRTFTGIDAL
                     WWAGEAERLGAGEICLNSIDADGVQDGYELDLTALISENVRIPVIASGGAGKPEHLAD
                     VLTKGRADAALIASMVHYRTYTIREIKQYLSGQNIAMRMFW"
BASE COUNT         2395 a         2464 c         2483 g         2638 t
ORIGIN      
        1 gggataaagc tttccctgct ttttgacgaa aagataatgc agcagaaggg ctgtcccgct
       61 gtatgttcgc aatggcatca agaaattggc ggcggcctgt gggcgtgaat actattctca
      121 aagttttgca gtctcaagat aaaggtcggc ctctctcagt acttcgtcga gctcataacc
      181 agttcccgca gctatttctt tctcgcctct ggcgagcagg tgtaaaatat ccatttcatg
      241 ctgggcattt ttatagactt ccatgcttac cattacagca gccgctcttc cacgctgtgt
      301 aataaaaacc ggttctcttg aagatgaaat actcttcacg acatcgctgg cattttgtct
      361 aagatcggaa atgggtataa tttttggtgc atttggcatt ggttgtacct cctattgtat
      421 tgtcaattgt actaccaatg aatgctttag tcaagcccgg ttgtgatatt ttcccctatt
      481 ttcttcgggt aacgcaaaag ctcacgggga ggcgtgggca acacaccgat cccgtgaagc
      541 gcttcgttag cggcttcaaa tctattcctt tatgatttcg acgaccttat cgattacttg
      601 gttaagtttg attatcttta tgttgcctat gcggtaaaga atgatgtgat tatctgcggt
      661 gaaaagtctg ttgggcctta tgttgctctc ctggtttaga ctgccagact cgaaatcgtt
      721 ttcatcgaca ggaatagcat aattgtcctt gatgcttttg cttgttatct ggcagagaat
      781 gagatcgtct ccttgaagcg cagccagaac aagagccggg cgtcttttgg attggctcag
      841 atcagaaaag gggaagggaa tgacaacaac atcccccttt acaaactttg ccatgcgcca
      901 tcctcctcct gcttcagcca gtcttttgct aaagacgatt cgctcatcac agcggtatcc
      961 aacttttctc ggcgggcttt tgctttcagg aaatgaacaa aatccagcac ctcagagaga
     1021 aacggctctg gagttttctc gatctcgctt ataagttcct ctttggtatt catggcaaca
     1081 tcctctcttg ctaattactt cttttcttta agcataccat cgatctccta acttgtatat
     1141 tcagaaattc ggagaaggct ctttcaaggc tagagatcca ccttgaggtc ttcagttgtt
     1201 atcgaaatct gcaagacacg aagccatact cgtcagtcat atttataccg ccaacgacga
     1261 agtgagccgg agcgcaacga tcggctctac tgatttgttg gctgttttta ttgtagtctt
     1321 gcaacttatt cgtaatgcgc ccacattcta ataattttta cagtgtggat atctttcaac
     1381 acttgataaa ttaagcgatg ctgtatgttt attcttctcg agtacgctcc cgaaagatca
     1441 ccgatgagct tttcatagtg tggtggattt tgaaatggat cttccttgag tatgtccaat
     1501 aggcggcttg cctggggttt tagaccggat tgtgaaatat gctttgcatc tttttgtgcc
     1561 tgtttagtga agactaattt ccaactcacc agccgggttc ctccttgcat ttttcgaccg
     1621 gagtttttaa accttttttg atggactccc gcatacctgg aattgacgcc aagtataatg
     1681 tttcttctat agctcgccag tcttcctcgg ataccagcac ggcggaattt cttttaccga
     1741 caatctgaac cggaacatgt gaatctttaa cttcgtccac aaggttgtat agacgttttc
     1801 ttgcttctga tgctgtaaat gttgtcattt ttcgtacctc cataacgtac gacatgcagt
     1861 acgccaccat tgggcaaatg tcaaattatt ttaagggcaa cgaaaaaatc agccggagcg
     1921 cagcgatcgg ctgtatgtat ttgttgggtt ttattttttt ggaaagtact taaccttgct
     1981 tatattttta aaatctgaat cctgagtcca tataatggct ttgaattctt gcgctgttgc
     2041 taaaatgatg ctgtccgcca ttggaagttg atgctctaag cttagccttg aagccgctat
     2101 tgccagttgt gtgcttagat ccacaacctt tcctttttgc atagcaacga ccgcctgcaa
     2161 tgcttcattt tcgctggact cccgcagaat gaccttgaag acttcgtata ttgttatcac
     2221 tggaacaaca agtaaatccg aatcactcag tggaggaagg aaatgcttgg cattaggctc
     2281 atcggcgaag tatgcaagcc agccggaaga atctacaata ttcataccct atcttcctct
     2341 cgtttgaatt cagtgttgat gcccttgaga aatccacgaa gttctttgat gtcgcgttcc
     2401 ggtataagtt caatacgacc ggcatattca acaatttgca ttttttggcc gggatgaaga
     2461 tttaatgcct ccctgacggt ttttggtata acaacttgat attttggtga tacggttact
     2521 gattgcatgc caacacctcc atcgatctat tttgtgctac gatatcatga tcgatatggt
     2581 tttgtcaatg atattcatac ccaacacaaa gataaccggg agtggcccgc aagggccgcg
     2641 atccggttca ttgacttgtt aaacgctttt gttttgcctt tcctttcctg tacgccatag
     2701 gaagtatgaa gcagcacagg agttttttcc agcgtgatat agcgtaagcc ttgaattcaa
     2761 agctacggcc gcacttgttg cataccgatg cataccacga gaacggcgtg ccgcaatcag
     2821 ggcacaacca gcgctttttc tgttcatcca gccaatggtc aatgccgttc cgtctaattg
     2881 tctcgagact ggacgccgat tcgcgagtat ggggtagaaa cttcgccagc gattgccatt
     2941 tgctataccc agcacacgga taatcagcgc agtcgataca atgctctaca tttttctccc
     3001 gtgcgcaccc acgaagttta catatgctgc acccggcata cacgttgtcg gatttacatc
     3061 caccacatat gtcaatgatc ggcataatgg agccaccgat tttcgccgta atggagccag
     3121 tgaaaagggt tgaaaaaggt cattggaccc ccctttcaat caggcgattt gacctccttt
     3181 cttggtttgt ttttctgttt tttgacagct tgtattttgt gtttccgatc tgggatgtgc
     3241 gcggtagctt tttccatcca gaacaatttt ataggcgccg tggcggaggc ggtcgatggt
     3301 ggccgcgccc aggatgcgat tggggaaggc gtcgggccat tccgtgatgt ccaggttgga
     3361 ggtgacgatg gtgcttttgc gttcatagcg ttcggagatc acctcgtgga agtcctcatc
     3421 ctggatgccg gttagtggtt tgaggccgaa gtcgtcgatg atcagcagat cgatgccgac
     3481 gagtttggcc aactgtcttt cgtaactgtt ggtggcccgg gcggcgttga tctgggcaag
     3541 catccgggag gcggtggtga aaaggacgtc atgaccggca cggatggcgc tgtgtccgag
     3601 ggcctgggca agatggcttt ttccggtgcc gcaggggccg acgatcaaca cggagacctt
     3661 ttcggccata aaccggcagg aggccagctc catgatcagg gcgcggttga tgttgggatt
     3721 gaaggtgaag tcaaactcct ccagggtctt ctggttgcgg aagttggccc ggcggagggc
     3781 cgaggccagt cgtttctgag accggcgggc gatctcgtcg tggacgatgg tggctaggaa
     3841 atccatgtag gaaaactgat tctcgatggc ttgacggttg cgggattcga tggaatccag
     3901 gataccggac agccgcaatt gcttgagcat gggaatcaat tcgggcatgg ggttcatcat
     3961 cgtttcctcc tttctcaatg gtaccgcatc acaccgggtt ggcggaggaa acgaccactg
     4021 ccggtgtaga cggaggaaag aggaacagtg tcacctggga ccgacgtttg atccaggcct
     4081 ttttccagaa tggtcttgac cgtcttgtaa cggggatttt cgaagtgcag cgcccgcttg
     4141 caggcagatt ccagacgcac aggcccgtac ttctttccca gaccgacaac cccttgggcg
     4201 gcgcggagat tgtccagaac ccggtcggca aagagcgtcc ggatcatttg atggcaggcg
     4261 ggtccgatgg cctccgcctg gcgcaggcac catcggggat cttgaagctt gtaagcgatc
     4321 gcctccggcg gcaggtgctc atcgacggtg gaacgggcgc cgggtttcct gagccgggga
     4381 tggacggcta ccagtttcag atcatgataa atctttacgg cgctgtccgt ggccttgatc
     4441 cacatggtct gatggatcag cgtaaacggc gccgagtaat acgctttctc aaactgaaca
     4501 tggcagttgg cgtgcagctt gacctggcac cagacggcga tctccacggg aacatcgggt
     4561 agaggtttca gcagatgcct ttccgcttcg gcaaaaagac tcaacggttt ctgtcgagtg
     4621 gtgccgtgaa tgcgattccc cgccgtttcc agaatccatt gacgcaactg cccgttggcg
     4681 tcagccagcg agcgaaactg acgcagggga acaaagcgat tcttgacata cttcacaccg
     4741 gattccacgc gccccttctt ctgaggatcc cgcggcgggc agggagagat cagaaatccg
     4801 tatccctccg ccaactcacc gtaagatcgt tgcacttccg gatcccggaa acaggacttg
     4861 gtgatggcgc acttgggatt atcaataatc accttggcag gcacgccatt gaagaactcg
     4921 aaggcccgcc ggtggcagga aagccaagtg ccaaccttct gatccgtgac aatctcggcg
     4981 taaagatgac ggctgaagca cagggtcatg acaaaaatcc aggtcttgaa agtctgaccg
     5041 gtgaaggcgt cggtgatgtc cggccccttg ccgaagtcca cctgcaccgc ctcgccgggg
     5101 gcaaaatcca gaatgcaggt cgcctcgggt tgatggagtt tcagcctctg caccagccgg
     5161 cgtacagaag aatagcttcc ggtaaatccg aattgatccg ccagggtctg gtgaatcgtg
     5221 gtcatgcaga cgtttccctc aacccacttg cgaatctgtt cttcataggc gccactcaaa
     5281 gactgatgcg tgggattgtt atcatgctct ttcttttgct caaatgccgc ccccagaacc
     5341 tcgtcgtcag gaaggggcag ccctcggtca agccacccct gctccccagc aatggcccga
     5401 acctgtccac attttatacg gcccatcagc cttgcccggg caatggtccg gtcactttct
     5461 cccatgcgca tcctgtgtat cacttgacga tattggtaca tctcgaacct cctgttgctc
     5521 atgtcagctc ctcccagcgt tgtattgcca cgagtatact gacgttcatc acatggtccg
     5581 agaccttttt taaacccctc ggtagaaaac ctcaccccaa atgggtggct ccattacggc
     5641 gatcctatac tggctccatt gcgccgatca tccaccggtc ccattacggc gatcatgaac
     5701 tggcctcatt acggcgatca cagattggct ctatagcacc gatcagtaac aacatacaag
     5761 ctcctctctc gggacatctc caaggcaggc cgcaaatccg tcagcataac cagttctacc
     5821 gtacatagct atcgagcagg caccgcagta gattccgcag tacgtatcag gattgaatgt
     5881 gttcatcttg cttcaccttt ctatgttggc taatgttcag ccccaccctg tttctcattt
     5941 gcttcgttta acgaaaagct caccgggagc atgccgcagg cacgcgatcc ggtggagcgg
     6001 cttgttcgga ttttttgtat aaatagtatc gaaaaccttg tggagcaacg cctgagccaa
     6061 ttgccgtagt accgtcaaga cgtggtatat cctcagaatc gtgtggataa ctaaaaacca
     6121 agaagtaagg cgtacagttt gaaggaaaaa ctttattcac atcactcttg acatatgaaa
     6181 tcccaccagg tgcgattgtt ttaatttcga ttattagtct ttgatttgat acttggaagt
     6241 caagatcgta tttcttgccg ttattggatt tcagccctat tttaatgtct gtaacatcac
     6301 gctggtgcaa tagccaatgt gagtatgcag tttgtataag ccattcaaag ccgtataaag
     6361 cagttgcttg cctgttatcg gaaacgaatt taaccttttt caatgaacaa caaagccaat
     6421 cgccttcctc tgcaacaaaa ctatcgacag cttctttaat ccattcatcc attgccatgt
     6481 tcatttcctt ttcaaaacca atcgaccggc gctccgttct acctctttgt attggcgtaa
     6541 cttgttaagg gctgctacgc cacctgccgc aactccaatt gatatagcgg caagggccgc
     6601 aggtaaaccc agtattccca cagcagcacc tccaacaagt gcagctccag gtgtaccagt
     6661 accacccgtt ccgatcacta ttattatggc aacagcaata gccccagcag cgattgccca
     6721 agcgaccttt cctgttgccc ttatacggac aacttttttg actaaatcac cttcgataat
     6781 aatcgtatct ttttcctctt tgagagcatt tcccagttct ttttcagaat tgatcgtgtc
     6841 ggacattttt attcctccga acatttaata taccaacttg gtttctgccg attttcgaag
     6901 gaaacattac tcctttttaa aacgaattca aagaaaaaag tgccttaaaa tccaataaaa
     6961 tcaacttttg ccacttggta ttttgtggta atatcccact taagccaagt tggcatatca
     7021 tggaaaattt gtcagactgc aggaaaaatg acgttcttcc ggagtttcag gcctttcttt
     7081 tggataagaa attggcgcag gaaaagaacg tcttttttta cgctttgtgg gccagtaaat
     7141 ttctaaacta ttccaataaa aagcagatac cacccgatca gtatcaggaa aacgccgtca
     7201 tcgaatttat cgaaactctg aaatcaaatt ccgccgtatc cgactggcaa atcaggcagg
     7261 ccggtgacgc cattcgttta tattattttc attatcgcgg cttcaaaccg agcaatttgc
     7321 cggcagggca atccgccggc tttgcgcagg agctgcttac ggaagcaaaa cggttgattc
     7381 ggcttaagca ctactcctac agcacggaaa aaacctatct gcagtggatt aaaagatttc
     7441 tggattatgc cagacaagcc ggcggaaaag aaaccgttgc tgaagttgga gcggttgact
     7501 gtaaaaattt tctcagccat ctggcgcttc aggaaagagt gtccgcatca acgcagaatc
     7561 aggcattcaa tgcccttctt tttctttttc gccatgtact ttgcaaagat atgggcaata
     7621 tggcggaaac tgtccgcgcg aaacgcggtc agaaactgcc cgttgtcttt tcagtagaag
     7681 aggtaaaacg attattatcc tgtctgacgg gcagggattt acttgttgcc ggtttgctct
     7741 acggttcggg gatgaggttg atggagctgg cccgcctgag ggtaaaagat gtcgatatgg
     7801 attttaacac cttaaccgtg cgcagcggaa agggcgacaa agacagaaca accattctgc
     7861 cggcaacggt caaagatcag ttgaaaaatc atctggccga agtgaaaaaa ttgcacgaaa
     7921 atgatctgac ccgcggatat ggcgaagtcc atcttcccga tgcgctgtcc agaaaatatc
     7981 ccaacgccgg taaagagtgg gcatggcagt atgttttccc ggccaataat ctatccgttg
     8041 atccgcgttc cggagcagtg cgccgccatc atatcagcga tacggccatc caggggatga
     8101 tcaaaaaggc aatccgcaag gccgggattc ccaaatttgc cacggtgcat acgttacgtc
     8161 atagttttgc gacgcatctg ctgatgaacg gcgtaaatat ccgtgaagtg caggagttgc
     8221 tcgggcataa gaatgttgaa acaacaatga tttacacgca tgtgcttcgt gatatgtcgc
     8281 acgcgccgca aagcccgctc gatacgttgc tggccggggc aaaaaatcca agctaaaatc
     8341 gttgatcact ttggatgcct gaaattgctc ttggcgtttg ggtattaaat ttgctagtag
     8401 aattgggtgc gcaggaatat tttttaggga atttgagtaa acatgatcgc aattatcgaa
     8461 tataatgccg gaaatattac cagtgtggcg agggcgctcc attccatcgg ccgggatttt
     8521 atcattaccg atgatacaaa aatgctcgat gcagcgtccc atgcgatttt tccgggtgtc
     8581 ggcgcggcgg gcgaggccat ggcgtacctc agggacaaaa agctggatat gtggctcaaa
     8641 cagtgttttc gatccggaaa gccgatcctg ggcatttgcc tgggcacgca gattattctg
     8701 gaggcctctg aagaaaacaa cacgcagtgc atcggcctgg tcggcgggac cacccggcgt
     8761 tttccggaga gcctgaccgc ggacggccgg gcgctgaaaa ttccccacat gggatggaac
     8821 agcgtcgcct tccggcgtcc gcatccggtg tttgcctgtg ttccggaaga agctgaattt
     8881 tattttgttc attcctttta tccgtcacct gccgatccgt cggctgtgct ggggaccacc
     8941 gaatacggca tgaccttctg ctcggtgctg gcggtcaaca atctggtcgc cgtgcagttc
     9001 cacccggaaa aaagcggccg gccgggcctg caaattctga aaaatttctg cgcgtggggg
     9061 ggaaaatgat ctccaaaaga atcattccct gcctggacgt ccgcgacggg aaactcacca
     9121 aaggtattaa atttaaagga aatatcgata tcggcgaccc ggtggaagcg gcccgcgaat
     9181 actacgaaca gggggcggac gaaattgttt tctacgacat cacggcctcg tccgacaagc
     9241 gggacattat gatcgatgtc gttcggcgcg tggccgagac gattttcatc cccttttcgg
     9301 tcggcggcgg catccgcagc ctcgacgaca tgcgccgggt catcggggcc ggtgcggaaa
     9361 aagtcagcgt caactccgcg gcggtgcggg agcccgccgt catatcccag ggtgcgcggg
     9421 cgttcggcag ccagtgcgtg gttctgggaa tggacgtcaa aaaaacggct gtctcaaaga
     9481 agaccccttc cggctatgaa atcgtcattg agggcggcag aacctttacg ggaatcgacg
     9541 cgctttggtg ggccggagag gccgagcgcc taggcgcggg ggaaatttgc ctcaattcca
     9601 tcgacgctga cggagtccag gacggctacg aactcgattt gacggcgctg atttcggaaa
     9661 atgtccgcat tccggtgatc gcctcgggcg gcgcgggcaa gccggaacat ctggcggatg
     9721 tcctgacaaa aggccgggcg gatgcggcgc tcatcgcctc gatggtgcat taccggacat
     9781 ataccatccg ggagatcaaa cagtacttga gcggccaaaa catcgcgatg cggatgttct
     9841 ggtagggcga aggattctgc ttgacgaaaa tccgtttgta ttcttaatat cccgttatta
     9901 tattacatca tatttttgca aaacgagaag agacgatttt acaaagtcag gaggcgtata
     9961 aaaagtggat gcaaaaaggt
//
LOCUS       DAOSME010000075         9969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_7701_length_9969_cov_19.657353, whole genome shotgun sequence.
ACCESSION   DAOSME010000075 DAOSME010000000
VERSION     DAOSME010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_9969_cov_19.657353"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..518
                     /locus_tag="PLG98_09650"
     CDS             171..518
                     /locus_tag="PLG98_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOE33566.1"
                     /translation="MPRPKCHRHICGIPDKNYFKPRGIPSVDLQEVVLSLDEFEAIRL
                     ADHEQVYQEEAATRMNISRQTFGRIIGAAHKKIADVLINGKALKIEGGEVTLSETGSC
                     DSAKSRRAGKNNH"
     gene            534..896
                     /locus_tag="PLG98_09655"
     CDS             534..896
                     /locus_tag="PLG98_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE33567.1"
                     /translation="MKIALPSNQNQVDEHFGHCEYFTVFTIDDQNKILSQETITPPPG
                     CGCKSNIAQTLAQMGVKYMLAGNMGQGAVNILGSNGIQVLRGCSGDVKEVVESWLKGA
                     VKDSGLACAAHEHGCHEN"
     gene            910..2262
                     /locus_tag="PLG98_09660"
     CDS             910..2262
                     /locus_tag="PLG98_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE33568.1"
                     /translation="MKVRREGMPDPFDHLGGQNIILDSIADGVFTVDHDWRITSFNRA
                     AEKITGVARDEAIGQLCKDVLKADTCERSCCLRATMKNGEPIVNKKVYIIDAQGRRLP
                     ISISTALLHDAKGKLLGAVETFRDISVEEDLRKAIRAKYSFADIISKNHRMLRLFDIL
                     PDIAESASTVLIEGESGTGKELVARAIHNLSARKRQPFVAVNCSALPDTLLESELFGY
                     KAGAFTDAKKDKPGRFQLAENGTLFLDEIGDITPAMQVKLLRVVQEKTYEPLGATKSI
                     EHNVRLIAATNKNLEDLVRQDRFREDLFYRINVFKVSLPPLRERMEDIPLLAEHFIAR
                     FNTLQKKDITGLSDEAMTVLMSHIYPGNIRELANMIERAFILCKGGLIEKKHLPESLF
                     ANQAIQDDLASLREAASACLMSALKQNNWNRLKTAKQLGMHKSTLYRKIKSLELSVPK
                     "
     gene            2471..2863
                     /locus_tag="PLG98_09665"
     CDS             2471..2863
                     /locus_tag="PLG98_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE33569.1"
                     /translation="MRIALSVWKDCISTVFDAADQLLVVEKDGDGVLKRTPFRLSSAD
                     GASRAMQFKEMKIDVLICGAISRPQEAAIAASGITVHPFVRGSVQEIIDAYESGQLHT
                     ATFALPGCHGRGFGAGRGKQRGKGCPWK"
     gene            2922..3470
                     /locus_tag="PLG98_09670"
     CDS             2922..3470
                     /locus_tag="PLG98_09670"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOE33570.1"
                     /translation="MNQYIPKEIFFTKGVGTHKEQLHSFELALRDAGIEKCNLVQVSS
                     IMPPGCRIISRAEGLKKLQPGAITFCVMSRCCSNEPKRLISASVGCAIPVDKKAYGYI
                     SEHHAFGYTERQSGDYAEDLAAAMLASTLGIDFDVDESWDEKKELFKISGKIVGTRNT
                     TQSCVLKNGNYTTVLAAAVFVF"
     gene            3498..3704
                     /locus_tag="PLG98_09675"
     CDS             3498..3704
                     /locus_tag="PLG98_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33571.1"
                     /translation="MNDANRSGRGNGGGQGMGGGRGRKGGPIAGGAVGTCRCPNCGHT
                     QAHERGVPCTQVQCPKCKTSMVRE"
     gene            3737..4099
                     /locus_tag="PLG98_09680"
     CDS             3737..4099
                     /locus_tag="PLG98_09680"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOE33572.1"
                     /translation="MPRGDSTGPMGMGPMTGRQAGYCAGYNMPGFVNNAGRRGTGMGF
                     GRGANFGGRGGFRRRNRFIATGVSGRAPFGNFAASFQRTDPETEKQALKSQAEYLQSE
                     MDAIKKRLDELNAKAPAK"
     assembly_gap    4191..4290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4373..4816
                     /locus_tag="PLG98_09685"
     CDS             4373..4816
                     /locus_tag="PLG98_09685"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOE33573.1"
                     /translation="MKRIGIIICARYQNCGGGKCFRALRERQGGFARYDSDEAVEIVG
                     YSTCGGCPGGNVEYVPAEFLKNGCDIVHLATGLVVGYPPCPRLRQFKIFIEEQFKLPV
                     VVGTHPIPLKYLETHGSLPFWKQTDMKELASHLMTESRELMESYN"
     gene            4839..5201
                     /locus_tag="PLG98_09690"
     CDS             4839..5201
                     /locus_tag="PLG98_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE33574.1"
                     /translation="MKIALTTSGKDLTAPLDGRFGRAPLFLIYDLETKAFEIVDNEQN
                     LNAAQGAGIQSAQNIARQGVKALITGHCGPKAFRVLQAAGIKIYNTNTATVSEAIDLY
                     SAGKLAEAASADVEGHWV"
     gene            5198..5692
                     /gene="tsaA"
                     /locus_tag="PLG98_09695"
     CDS             5198..5692
                     /gene="tsaA"
                     /locus_tag="PLG98_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOE33575.1"
                     /translation="MNPCDILYWTIGIIHSEHTIAEKTPIQPVYAKGCKGYVELWPDL
                     APGLKDLDDFSHIYLIYHFHKTQEAKLIVKPFLQDVDRGIFSTRAPFRPNPIGLSVVE
                     LLGIEGNILHLNNVDILDGTPLLDIKPYTAKFDLHNVKKNGWQDEVDEPTAQKRGKRG
                     YSRP"
     gene            5689..6576
                     /locus_tag="PLG98_09700"
     CDS             5689..6576
                     /locus_tag="PLG98_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOE33576.1"
                     /translation="MKTSLDCIPCFVRQAADAVKMSFSSDEERNRLMRNVLQWLGEID
                     PDQSPPAISQIIHRRVRGLLTMDDPYRAAKDSHNALAAALLPAIRRRIGESFDPLSMA
                     VRYAIMGNIIDLGAKNGVGFGDIYAELQSAYMQPIWGDMLAFEKAAASAQTILYLADN
                     AGEIFFDRLLIEQLHGSKVTLAVRGALVINDATRADALAAGLEDIVNIIDNGSDAPGT
                     ILNDCSAEFRRHFDEADMIIAKGQGNFESLSDEPCNIFFLFRIKCPVISRHSGFSVGT
                     YVAANRFQHRDIPAERGVQ"
     gene            6573..7220
                     /locus_tag="PLG98_09705"
     CDS             6573..7220
                     /locus_tag="PLG98_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33577.1"
                     /translation="MKASVIAIELKAHAPFTALGTLTGIVIMAAFIQYQVPKEISSTL
                     FWTLHPLHVLISALVTTAMYRMYTGGGIWRTILIGYFGSVGIATLSDSLIPFAGEWLL
                     DLPYRGIHLGFIEKWWLVNPLALAGIALGYVISHTKIPHAGHVLLSTWASLFHIIMAL
                     GTSIDVVTTILITVFLFLAVWIPCCTSDIVFPLLWIKEGNGNGKHPVSFSDGGTP"
     gene            7217..8065
                     /locus_tag="PLG98_09710"
     CDS             7217..8065
                     /locus_tag="PLG98_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33578.1"
                     /translation="MKIAIASGKGGTGKTTVSVNLARTMGVPVQLLDCDVEEPNAHLF
                     LAGDKIKDGIVTIPVPQVDEALCDGCGECSKFCEYHAIVTFGTTPLIFPEMCHGCGGC
                     TRVCPKKAITEVGRRIGVVETRRSENITLIGGQLDVGVAMAPPLIRAVKERLQKNLPA
                     ILDAPPGTSCPVIAAIRDTDLVLLVTEPTPFGLNDLSLAVDMVRELHIPFGIIVNRMG
                     SGDERVQIFCQKEGIPILLEIPDDRRITEAYSRGILMVDALPEYRQLFESLTEKISKL
                     GKKGRT"
     assembly_gap    8077..8176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8177..8398
                     /locus_tag="PLG98_09715"
     CDS             <8177..8398
                     /locus_tag="PLG98_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE33579.1"
                     /translation="VQFEKQQSMKDEPLTMCPECGGNVQRLVSGGSGFILKSQGQSLR
                     DSRKSCSLEETGKTCCGASQRCGESHCGD"
     gene            8403..9359
                     /locus_tag="PLG98_09720"
     CDS             8403..9359
                     /locus_tag="PLG98_09720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33580.1"
                     /translation="MSPSDFKYLYGPVPSRRLGRSLGIDLVPFKTCTYDCIYCQLGRT
                     TNKTMERRGYTPVKDILDELKLKLASGDSPDYISIAGSGEPTLHSYIGELIGMIKNMT
                     TIPVAVLTNGSLLYLPEVRAALMRADLVIPSLDAGDDTMFRYVNRPHNGISFGNMVNG
                     LIDFIDYFPGQVWLEVLLVSGVTGMAEDVKKIADLAKVIGANRVQLNTVCRPAAEDYA
                     CAVERSQMENLARLFTGEVNIINDDESFDLSVIGAKDTANEEIVSLLARRPCTLTGIC
                     AGLGLHPHDAAKKLKKLVDEKRITTLRAGHDVFYKIVEKGRS"
     gene            9356..9967
                     /locus_tag="PLG98_09725"
     CDS             9356..9967
                     /locus_tag="PLG98_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33581.1"
                     /translation="MTKEVIENHQRYLERVALYKQHGCDIEEDRSFIIEKACPFSGNI
                     LEAGTGKGYFTLALARLGFHFTSFDISDAEQQYAKLNLAYYGLTSYVCFDVANAECLP
                     YQDNLFEVIFAVNIVHHLPSVRKVCDELSRVLSPTGKIVLSDFNANGFALVDKIHALE
                     GKRHELNAGTLAETKAVLTEYGMKVEEYHDTYQDVLVARRIIL"
BASE COUNT         2534 a         2383 c         2591 g         2261 t
ORIGIN      
        1 ctgaaagagg taaatcgtta tcctcgatta aattaaaagc tttcaaacaa taatcattaa
       61 ctatatcggc ctgatagttt accgagcatt ttccaaagca aaaaacaata ttatctcttg
      121 acttattatg agcatatgct tattattctc gtcaaaggag aatgataagc atgccccgcc
      181 ccaaatgcca tcgtcatatc tgcggaatac cggacaaaaa ttatttcaag ccgcgtggaa
      241 taccttctgt tgatctgcaa gaggttgttc tgagccttga cgaatttgaa gcgattcgac
      301 tggccgatca cgaacaggtt tatcaggaag aggcggccac tcgaatgaac atttcgcgcc
      361 agacattcgg cagaatcata ggagcggcgc acaaaaagat tgctgacgtt ctgataaatg
      421 gaaaagcact caaaattgaa ggcggcgaag tgactttaag cgaaaccggt tcttgcgaca
      481 gtgcgaaaag ccgcagggct gggaaaaata atcattaatt aaaggagaag atcatgaaaa
      541 tcgcgttacc atctaatcaa aatcaggttg atgaacattt cggacactgt gaatatttta
      601 ccgtattcac cattgatgac caaaacaaga tcctttcaca ggaaaccatc accccgcccc
      661 ccggatgcgg ttgcaaatca aatatcgctc agaccttggc gcaaatgggg gtgaagtata
      721 tgttggccgg aaacatggga caaggcgcag tcaatattct ggggagcaac ggaattcagg
      781 ttttgcgcgg ttgttccggc gatgtgaaag aagttgtgga aagctggctt aagggcgccg
      841 tcaaggattc gggcctggcc tgcgcggccc acgagcatgg ttgtcatgaa aattaactaa
      901 ggactcgata tgaaagtccg acgcgaaggc atgcctgatc ctttcgatca tctaggcggg
      961 caaaacatca tcctcgattc tatcgcggac ggtgtgttca cggtggatca tgattggcgc
     1021 atcacgtctt tcaaccgcgc ggcggaaaag ataaccgggg tcgcgcgcga tgaagccatc
     1081 ggccaattgt gcaaagacgt cttgaaggcg gatacctgcg agagaagctg ctgtctgcgc
     1141 gccaccatga aaaacggcga gccgatcgtc aataaaaaag tttatattat tgatgcgcag
     1201 ggccgacgcc ttcccatcag catttcgacg gcgcttttgc atgatgccaa agggaaactt
     1261 ctgggagcgg tggagacctt ccgtgacatc agcgtcgaag aggatttgcg aaaagccatc
     1321 cgcgcgaaat actcctttgc ggacatcatt tccaagaatc accggatgct tcgccttttc
     1381 gacatcctgc cggacattgc cgaaagcgcg agcaccgtgc tgatcgaagg cgaaagcggc
     1441 acggggaagg aactggttgc ccgggccatt cacaacttaa gcgccagaaa acgccagccg
     1501 tttgtcgcag tgaattgcag cgcattaccg gacacgcttc tggaatcaga actctttggt
     1561 tacaaggcag gtgcgtttac cgacgcaaag aaggataagc ccggccggtt tcaactggcc
     1621 gaaaacggca cgctgtttct tgatgaaatc ggcgacatca cgcctgccat gcaggtgaag
     1681 ctgctgcgcg ttgtgcagga aaaaacctac gagccgctgg gtgctacaaa aagcattgaa
     1741 cacaacgtgc gcttgattgc ggccaccaac aaaaaccttg aagatctcgt ccggcaggac
     1801 cgctttcggg aagacttgtt ttaccgaatc aatgttttta aagtcagcct gccgccactg
     1861 agagaacgca tggaagatat tcccctgctt gcggagcact tcattgcccg cttcaacacc
     1921 ctgcaaaaaa aagacatcac gggcttaagc gacgaagcga tgaccgtatt gatgtcgcat
     1981 atctatccgg gcaatattcg cgaactggcc aacatgattg aacgggcttt cattttgtgt
     2041 aaaggcggcc tgatcgaaaa gaaacatcta cccgaatctt tattcgcgaa tcaagcgatc
     2101 caagatgact tggcatcttt gcgggaggcg gcctcggcct gtctgatgtc cgcacttaag
     2161 caaaacaact ggaaccgcct gaaaaccgca aagcaactgg gtatgcacaa aagcaccctt
     2221 taccgtaaga tcaaatctct ggaactttcc gttccgaagt aagccacaaa tatgaatgca
     2281 ataatgcgac catgtcgcaa ccataagtcg cattattgcg acttatggaa gtatttaacc
     2341 gaagctggtt aatcctatct atctaatata acggtgttat tttttagagc tgtttctggc
     2401 atccttcatg caaaatcaac ttacgaaaca caggtgaacg aaccgttcac tgaatcggaa
     2461 gcgaggattt atgagaattg ccctgtcggt atggaaagat tgcatctcga cggtttttga
     2521 cgcggcggac cagctgctgg tggtggaaaa agacggtgac ggtgtgctta aacgcacgcc
     2581 tttccggctc agctccgccg acggagcatc acgggccatg cagtttaagg aaatgaaaat
     2641 cgatgtgctg atttgcggcg cgatttcccg tccgcaggaa gcggcaattg cggcatccgg
     2701 aattacagtt catccatttg tgcgcggttc tgtgcaggaa attatagacg cttacgagag
     2761 tggacaatta catacggcga cctttgcgct acccggctgc cacggacggg gttttggcgc
     2821 agggcggggg aaacaacgcg gtaaagggtg cccttggaaa tagacgggat ttttgaagaa
     2881 tgaaaacacc agactgcata aaataataag gagaaaaaac attgaaccag tatataccca
     2941 aagaaatttt ctttaccaaa ggcgtcggca cgcacaagga gcaacttcat tcttttgaac
     3001 tggcgttgcg cgacgcaggg attgaaaaat gcaacctcgt gcaggtatcg agcatcatgc
     3061 cgcctggctg ccgcatcatc tcgcgggcgg aaggattaaa gaaacttcag ccgggcgcta
     3121 tcactttttg cgtcatgagc cgctgttgca gtaacgaacc caagcggctg atttccgcgt
     3181 cggtcggttg tgcgattccc gtggataaaa aagcctatgg ctatatcagc gaacaccatg
     3241 ccttcggcta caccgaacgg cagagcggcg actacgcgga agatctcgcg gcggccatgc
     3301 tggcctcgac actgggaatc gacttcgatg tcgacgaaag ctgggatgaa aaaaaagagt
     3361 tgtttaaaat cagcggaaaa atcgtcggca cgcgcaatac cactcaatcc tgcgttttga
     3421 aaaatgggaa ttacacgact gtgctggctg ccgcggtttt tgttttctga aaaggcagct
     3481 caaaggagga aatacttatg aatgatgcaa atcgcagcgg aagaggaaat ggcggcggac
     3541 agggaatggg tggcggtcgt ggacgtaagg gcggaccgat tgcgggaggc gctgtcggca
     3601 cttgccggtg tccaaactgc ggccataccc aagctcatga acgaggcgtg ccctgcactc
     3661 aggttcagtg cccgaagtgc aagacatcga tggttcggga atagctaaac aatcgcatga
     3721 aaggaggatt tttattatgc caagaggaga ttcaacaggc cccatgggaa tgggacccat
     3781 gaccggtcgt caggccggat actgcgccgg atacaatatg cccggttttg taaataacgc
     3841 cggcaggcgc ggtacgggaa tgggttttgg acgtggagct aatttcggag ggcgcggcgg
     3901 atttaggcgg cgcaatcgct ttatcgccac aggtgtgtcg ggcagagcac cgttcggaaa
     3961 ttttgcggcg tcatttcagc ggaccgatcc tgaaacagaa aagcaggcgc tgaaaagtca
     4021 ggccgaatac ctacagtctg aaatggacgc gatcaaaaag cgcctcgacg agctgaatgc
     4081 aaaagccccg gcaaaataat gccctcgatc caaaagttca aaaaggcatg gcactgctca
     4141 tccatgcccg gcggtggcgg tgtgtttcac tcaagcttca cactgccgcc nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgccgccac cgattgtaat cataacggag
     4321 gatggatcga tcttaaaaac cttctcccaa acgcttagac gaggaactga aattgaaaag
     4381 aatagggatt atcatctgtg cccgttacca gaattgcggc ggaggaaaat gtttcagggc
     4441 tttgcgcgaa cgccagggcg gttttgcccg gtacgattca gatgaagctg tggaaatcgt
     4501 cggatattcg acttgcggcg gttgtccggg cgggaatgtg gaatatgtgc ccgcggaatt
     4561 tctaaaaaac ggctgcgata tcgtccatct ggccaccggc cttgtggtgg gataccctcc
     4621 gtgtccgcgg ctccggcaat ttaaaatttt tattgaagag cagtttaaac tgcccgtagt
     4681 cgtgggaact caccccattc cgcttaaata tctggagact cacggcagtc tccccttctg
     4741 gaaacaaaca gatatgaaag aactcgcttc tcaccttatg actgaatccc gcgagttaat
     4801 ggaatcttat aattgatgcc aaagaaagag agaatgatat gaaaatagca ttaaccacat
     4861 caggcaaaga cttaaccgca cccttggatg gccgtttcgg gcgcgcgccc ctgtttctga
     4921 tttatgatct cgaaaccaaa gctttcgaga ttgtcgataa tgagcagaat cttaacgcgg
     4981 cacagggtgc aggcattcag tccgcgcaaa acatcgcccg gcagggcgta aaagctctga
     5041 tcaccggaca ttgcggtccg aaagcgtttc gcgttcttca ggcggcaggc attaaaattt
     5101 ataacacaaa cacggcgacc gtatcggaag caatcgacct ttatagcgcc ggcaaactgg
     5161 ccgaggccgc atcagccgat gttgaggggc actgggtatg aatccttgcg atattcttta
     5221 ttggacaatt ggcatcatcc acagcgaaca tacgatcgct gaaaaaacgc ccatccagcc
     5281 tgtttatgcc aaaggctgta agggatatgt tgaactgtgg ccggatttag cccccggact
     5341 gaaagacctg gatgattttt cgcatattta cctgatttat cactttcaca aaacgcaaga
     5401 ggccaaattg attgtaaagc cgttcttgca ggatgttgat cgcggcattt tttcgacacg
     5461 cgcaccgttt cgtcccaatc ctatcggatt gagtgtagta gagcttctcg gcattgaagg
     5521 caatatcttg catctgaaca acgtggatat tctggacggc acaccgcttc tggacatcaa
     5581 gccctacaca gccaaatttg atcttcacaa cgtaaagaag aacggctggc aggacgaggt
     5641 ggacgaaccg accgctcaaa agcggggaaa acgcgggtat agccgaccat gaagacgtct
     5701 ttggattgca ttccctgttt cgttcgccag gccgcggatg cggttaaaat gtcgttttct
     5761 tcagatgaag aacggaatcg cctgatgcgc aatgttttgc aatggcttgg tgaaatcgat
     5821 ccggatcaat caccgcctgc tatttcgcaa atcattcatc gccgggtgcg cgggcttcta
     5881 acgatggacg atccctaccg cgccgccaag gattcacaca acgcattagc ggcggcgttg
     5941 cttcccgcaa tccggcgtcg gatcggcgaa tcattcgatc ctttgagtat ggcggtgcgc
     6001 tacgccatta tgggcaatat catcgacctg ggggccaaaa acggcgttgg cttcggtgac
     6061 atatatgcag agctccaaag cgcatacatg caacccattt ggggagacat gcttgccttc
     6121 gaaaaagccg ccgcttcggc gcaaacgatt ctttatctcg ccgacaacgc cggggaaatc
     6181 ttttttgatc gtctgctcat cgagcaactg cacggatcaa aagtaacttt ggccgtgcgt
     6241 ggcgcgctgg ttatcaatga cgctactcgc gccgatgcgc tggcggccgg tcttgaagac
     6301 attgtgaata ttatcgacaa cggatcagac gcgccgggca ccattctcaa cgattgcagc
     6361 gccgaattcc gtcgccactt tgacgaagct gatatgatca ttgccaaagg tcagggcaat
     6421 tttgaaagtc tttccgacga accgtgcaat atctttttcc tgtttcggat caaatgcccg
     6481 gtcatcagcc gccattcggg attttccgtg ggaacttacg tggcggcaaa tcggtttcag
     6541 catcgggaca ttcccgcaga aagaggcgtt caatgaaagc atctgtaatt gccattgaac
     6601 tcaaagcgca cgcccctttt acggcgcttg gaaccttaac aggcattgtc atcatggccg
     6661 cttttattca atatcaagta ccaaaagaaa tttcatccac tcttttctgg acgctgcatc
     6721 cgctgcatgt gctaatcagt gccctggtga ccaccgcaat gtaccggatg tacacaggcg
     6781 gcggtatttg gaggacgatt ctcattggtt atttcggttc ggtcggcata gccactttaa
     6841 gtgattcgct cattcctttt gccggagaat ggctgcttga tctgccttat cgcggtattc
     6901 atctgggttt catcgaaaaa tggtggctgg tcaatccatt ggcgttggcc ggcattgcgc
     6961 tgggctacgt catatcgcac actaagattc cccatgcggg gcatgtgctg ctcagcacct
     7021 gggcgtcgct gtttcacatt atcatggcgc tgggcacctc cattgatgtc gttaccacaa
     7081 tactgataac cgtctttttg tttttagccg tctggattcc ctgctgcacc agcgatatcg
     7141 ttttcccctt gctctggatt aaagaaggaa acggaaacgg caagcatcct gtttccttct
     7201 ccgatggagg cacgccatga agatagccat cgcttccggt aaaggcggaa ccggcaagac
     7261 caccgtatcc gtcaatctgg cgcgcacgat gggtgtcccg gttcagctgt tagactgtga
     7321 tgtggaagag cccaacgcgc acctgtttct tgctggagat aaaatcaaag atggaatcgt
     7381 tacgattccg gttccccaag tggatgaagc gctttgcgac ggttgcggcg aatgcagtaa
     7441 attttgcgag tatcacgcca tcgtcacgtt tgggacgacg ccgctgattt tcccggaaat
     7501 gtgtcacggt tgcggcggtt gcacacgggt ctgtcccaaa aaagccatca cggaggttgg
     7561 caggcggatc ggcgtagtgg aaacaagacg ctcggaaaac atcaccctca tcgggggaca
     7621 gctcgatgtc ggcgtcgcca tggccccgcc gctgatccgg gcggtcaagg aacgcctgca
     7681 aaagaattta ccggccattc tggatgcgcc gccgggaacg tcctgcccgg tcattgccgc
     7741 tattcgggat accgatctgg ttttgctggt cacggaaccc acaccgtttg gcctgaatga
     7801 tttgtcactg gccgtggaca tggtgcgcga acttcatatc cctttcggca tcatcgtcaa
     7861 ccgcatgggc agcggagatg agcgcgtgca gattttttgt caaaaggaag ggattcccat
     7921 cctgctggaa atccccgacg accggcggat taccgaggcc tattcacggg gaatcctgat
     7981 ggtggacgcg ctgccggaat accggcaact ttttgagagt ctgacggaaa aaataagcaa
     8041 acttggaaag aaaggaagga cctgaaaaat gccaacnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnngtgc aattcgaaaa gcagcaaagt atgaaagacg agccgcttac
     8221 gatgtgtccc gaatgtgggg gaaacgtcca aaggctggtc agcggcggca gcggctttat
     8281 cttaaaatcg caagggcaaa gcctgcgcga ctcacgcaag agctgctcat tggaagaaac
     8341 cggtaaaacc tgctgcggcg caagccagcg gtgcggggaa tcgcattgcg gagattgaaa
     8401 aaatgagtcc aagtgatttt aaatatcttt acggtcctgt gccgtcgcgg cggttgggcc
     8461 gctccctggg aattgatctg gtgcctttta aaacctgcac gtacgattgc atatattgtc
     8521 agttgggcag gacaacgaac aaaacgatgg aacggcgggg gtacactccc gtcaaggata
     8581 ttttagatga gttgaagctg aaactggcat cgggagactc tcccgattac atcagcatcg
     8641 ccggttccgg cgagccgacg ctgcattcct atatcggtga gctgatcggc atgatcaaga
     8701 acatgaccac catccccgtg gccgtcctga ctaatggctc tttgctttat ctgccggaag
     8761 ttcgcgcagc tttgatgcgg gcggatctgg tcatcccttc gctcgacgcc ggtgatgata
     8821 cgatgtttcg atacgttaac cgcccccaca acggtatttc ttttggtaac atggtcaatg
     8881 gcctgatcga ttttatcgat tattttccgg gacaggtgtg gctggaagtt ctgctggttt
     8941 ccggcgtaac cggcatggcc gaggacgtaa aaaaaatagc cgacttagca aaagtaatcg
     9001 gggcaaatag ggtgcagctc aataccgttt gccgtccggc tgctgaggat tatgcctgtg
     9061 ccgtggaaag aagccagatg gaaaatcttg ccaggctctt tacgggcgag gtgaatatca
     9121 taaacgatga tgaatccttt gatctttccg ttattggcgc aaaagatacc gccaacgagg
     9181 aaattgtttc cctgctggcg aggcgtccct gtacgctgac cggaatctgc gcggggctgg
     9241 ggctgcatcc gcatgacgca gccaaaaagc tcaaaaagct cgtggacgaa aaacgaatca
     9301 cgacgctacg cgcgggacac gatgtgtttt ataagatagt ggaaaaaggc cgatcatgac
     9361 aaaagaagta attgaaaatc atcagcgtta tttagagcgt gtcgcgcttt ataaacagca
     9421 cggatgcgat attgaagaag atcggtcttt tattattgaa aaggcatgcc ccttttcagg
     9481 caatatcctg gaagcgggca cggggaaagg ttattttacg ctggctttgg cgcggctggg
     9541 ctttcatttt acgtcatttg atatttcgga tgcggagcaa caatatgcca agcttaacct
     9601 cgcatattat ggcctgacaa gttacgtctg tttcgatgtg gccaatgcgg aatgtcttcc
     9661 ctatcaagac aatttattcg aagtgatctt tgcggttaac atcgtccatc atcttccttc
     9721 cgtccgcaag gtctgcgacg aactgagccg ggttctgtca ccgaccggaa aaatcgtttt
     9781 gagcgacttc aatgcaaatg gattcgccct tgtcgataaa attcacgcgc tggagggcaa
     9841 gcggcatgaa ttgaacgcgg gcacactggc tgaaacaaag gccgtcttaa cagaatacgg
     9901 catgaaagta gaagagtatc acgacaccta ccaggatgtg ctggtggcca gacgtatcat
     9961 tttataaaa
//
LOCUS       DAOSME010000076         8985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_8726_length_8985_cov_37.110526, whole genome shotgun sequence.
ACCESSION   DAOSME010000076 DAOSME010000000
VERSION     DAOSME010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8726_length_8985_cov_37.110526"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLG98_09730"
     CDS             complement(<1..655)
                     /locus_tag="PLG98_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOE33582.1"
                     /translation="MALFEGYERRIKQITPFLAKYGISSLEEAEKVCLEKGVDVRKIV
                     KEIQPISFENAGWAYLVGAAAAIKKGQKVAADIAETLGEGLQSFCIPGSVADDRQVGI
                     GHGNLASMLLREETKCFAFCAGHESFAAAEGAIGLAKSANKARKVPLRVILNGLGKDA
                     AHIIARVNGFTHVATAFDYKTGKVSVAKEKAYSKGDRAAVRCYGADDVREGVAIMHLE
                     "
     gene            complement(667..1359)
                     /locus_tag="PLG98_09735"
     CDS             complement(667..1359)
                     /locus_tag="PLG98_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33583.1"
                     /translation="MEYTHEVEQMCVVAKGAKNGPAPIPQEGKWTMVKEIKDISGLTH
                     GVGWCAPQQGTCKLTLNVKDGIIEEALVECLGCTGMTHSAAMAAEILPNKTILEALNS
                     DLVCDAINTAMREIFLQIVYGRTQTAFSEGGLPIGAGLEDLGKGQRSVIATMYGTKAK
                     GPRYLEMAEGYVLEIGLDGDSEIIGYKFVNLGVMMDSIVKGMDPAQALSKATGTYGRF
                     AEAVKVINPRKE"
     gene            complement(1650..2399)
                     /locus_tag="PLG98_09740"
     CDS             complement(1650..2399)
                     /locus_tag="PLG98_09740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33584.1"
                     /translation="MDIPRIFNITESAHRIHNPITPEKLAILGSALRLESGDRVLDLG
                     SGSGEMLCTWARDHGIIGTGIDMSRLFTEQAKRRAEELGVADQVTFIHGDAAGFVSGE
                     KVDVAACLGATWIGGGVAGTIELLAQSLKLGGIILIGEPYWRQLPPTEDVAKGCLAHT
                     IADFIMLPELLASFDRLGYDVVEMILADQDGWDRYEAAKWLTMRRWLEANPGDELAKE
                     VRAQLTSEPVRHAAYTREYLGWGVFALMSRR"
     gene            2636..3232
                     /locus_tag="PLG98_09745"
     CDS             2636..3232
                     /locus_tag="PLG98_09745"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOE33585.1"
                     /translation="MKILDRKFYHRDTLRVARELLGKKLVRRINGLELSGMIAETEAY
                     CGRADSACHAHRGKTQRNAVMFGKPGHAYVYFTYGMHYMLNLVTEEEENPCAVLIRAI
                     LPIDGIAAMEARRKKKGTELTNGPAKLCQALSIDKSLNGWNVTLGSELWVENNKKIPD
                     SKIITTPRIGIDYAKTKDREALWRFILKKDDLEGRPVR"
     gene            complement(3236..4306)
                     /locus_tag="PLG98_09750"
     CDS             complement(3236..4306)
                     /locus_tag="PLG98_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOE33586.1"
                     /translation="MNKKPGYNFQNIGFIVLLVIITLFFFYIVKPFFFAVFWAALIAG
                     IFMPLHRALNTKITNPNLCAGATLVVILLCLIIPIAFLFNLLVLEAFDIYKSFDSQGG
                     AWMKTLTATLNTLSQSALFAKLNLDQAFILGKFQDIIKAVSGYVINHISNFTQNTILV
                     IVKVAVMIYTLFYFLRDGERLLESMVGHIPVNKRYLDTFIYQFLSTAKASLKFTFVIG
                     GIQGFLGGLVFYITGIERALIWGVIMMGLSILPGVGCAFVWVPAGIIMLVLGHTWQGI
                     VILIFGSVIISSVDNLLRPVLLGRDTNMHALLIFLSTLGGLAVLGLSGFVMGPIIAAL
                     FLASWKLFLEFYQEKQEEKIES"
     gene            4652..4852
                     /locus_tag="PLG98_09755"
     CDS             4652..4852
                     /locus_tag="PLG98_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33587.1"
                     /translation="MALDEPAKDDEVFDENGTKFVIDKDIFNQAKPINVDFIETPQGS
                     GFKVTSNLSAAGGGCGSSCSGC"
     gene            4894..5832
                     /locus_tag="PLG98_09760"
     CDS             4894..5832
                     /locus_tag="PLG98_09760"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE33588.1"
                     /translation="MTDEKSTKRDLAEKLLQKGKYDEAIVLLEDIHQESPDEDPVLLM
                     LAWAYYDSGKTGQAEKCLTILFDRELERKVFTGFAFDELVRLYKQKKDFPNLVNICER
                     AVAAQPDDVGLLTELGNAYLQAGQADRARLTYEKLIAMEDDNPAFYCLWGEALFAAGL
                     NKESEDAYRKAGQLDPDQLDSYYFRIAVLFQKADNHQEARRLLNKCLNLNPSSPLYYC
                     SLGDSLIGLGKLQDAINAYNQAVEFDRADADAYYNRLGNSLMKTGNFSEAVAAFQTAI
                     RLEPLPIYYLNLSSAYRKMGLDHEADQTIGKADKIH"
     gene            complement(5834..6346)
                     /locus_tag="PLG98_09765"
     CDS             complement(5834..6346)
                     /locus_tag="PLG98_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33589.1"
                     /translation="MNTDKLAPESVEEEIDYSGRPFDYRDMDTSSAMICENDPAIRQK
                     LRENLQTLNFNVIEPMTAKEALKYTTFQTFNVIVVNEHYDTDKDGVNQVLKYLETLPM
                     SVRRQVFVVLISSTFATMDYMNTLNRSVNLIIHMEEISEIGLILKKEMEENEYFYHVF
                     KELQRKLGRV"
     gene            complement(6384..7568)
                     /locus_tag="PLG98_09770"
     CDS             complement(6384..7568)
                     /locus_tag="PLG98_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOE33590.1"
                     /translation="MRKPEIDHILTKMLESYPNISDFIITVGKPFQVEAAGQLVPVPL
                     RPHFEKLTPFQTEIFALNLVNQDHRLMTDLLKEGACDLSYELTGVARFRVNIFSQMGN
                     YSIVLRKLESLVPSCADLGLPDAYYRISREKNGIVLVTGATGTGKTTSLAAIINEINE
                     TSSVHIITLEDPVEYTHPQKKATINQRELGKDFDTFSSGLRAAMRQAPKVILVGEMRD
                     RESVEIALSAAETGHLVLSTLHTVDAGQTINRIIGMFPRDEENQIRVRLADAIRWIAC
                     QRLLPKIGGGRVAAFETLSTSLRVKDVILHGESEGKTFNDIITQGRPFGMMTFDDCII
                     DHFSKDLITEETAIAFATNRAVVNRGIDAVKKARGDQAVNIGTLEVDRSYGKAKMDTW
                     KR"
     gene            complement(7590..8672)
                     /locus_tag="PLG98_09775"
     CDS             complement(7590..8672)
                     /locus_tag="PLG98_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOE33591.1"
                     /translation="MAKIDAFFNLMHDQGASDLHLVAGQQPAIRIRGDIERIKYDVLT
                     NDALKVLLYEITPEHKIKQFEETGDVDFAYEVPNLARYRVNFFRQKNGMAAVFREIPD
                     KIQTCQQLGLPNVIAKLAQLPRGLVLVTGPTGSGKSTTLAAIIDEANRLRRDHIITIE
                     DPIEFVHKSQNCIVNHREVGVHTKTFTAALRGALREDPDIILVGEMRDLETISLAIEA
                     ASTGHLVFSTVHTTSAPKTVDRIIEVFPASEQLQIRSTLADGLRAVISQVLFKRIDVK
                     SRCVALEICIATPAVRNLIRESKTYQIPSMMQTGKKYGMQLLDDAIMDLYNRGWISGD
                     DAYIKANEKARFRPLLRQPPTDFTEA"
     gene            8910..8985
                     /locus_tag="PLG98_09780"
     tRNA            8910..8985
                     /locus_tag="PLG98_09780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8943..8945,aa:Ala,seq:cgc)
BASE COUNT         2283 a         2238 c         2186 g         2278 t
ORIGIN      
        1 cttccagatg catgatggcc acgccttcgc gcacgtcatc ggcgccgtag cagcgaactg
       61 ccgcgcggtc gcccttggaa taggctttct ccttggccac agagactttt cctgttttat
      121 aatcgaatgc cgtcgcgaca tgggtgaatc cgttgacgcg ggcgataatg tgagccgcgt
      181 cttttcccaa tccgttcaga atgacgcgca gaggaacctt gcgcgccttg ttggccgatt
      241 tggcaagtcc gatcgcgcct tccgctgccg cgaatgattc atgacctgcg caaaacgcaa
      301 agcatttggt ttcttcccga agcagcatgg aagcgagatt gccgtgaccg atgcccacct
      361 gacggtcatc ggcaaccgag ccgggaatgc agaaggactg caatccttca cccagtgttt
      421 cggcgatatc ggcggctacc ttctgacctt ttttaatggc ggcggcagcg ccgaccagat
      481 aagcccagcc ggcgttttca aatgaaatcg gctgaatttc cttgacgatt tttctgacat
      541 ccacgccttt ctcgaggcat actttttcgg cttcctccag cgaggaaatt ccatatttcg
      601 cgagaaaagg agtgatctgt ttgattcttc tttcgtatcc ttcaaataaa gccatttttc
      661 tactccttat tctttacggg gattgataac ttttacagct tcagcaaaac ggccgtaagt
      721 tccggttgct ttcgaaagcg cctgggccgg gtccatgccc ttgacgatgc tgtccatcat
      781 cacgcccaga ttaacgaatt tgtagccgat gatttcactg tcgccatcca ggccgatttc
      841 cagcacgtac ccttcggcca tttccaggta acgggggcct ttggctttgg tgccgtacat
      901 cgtggcaatc acgctgcgct ggcctttgcc cagatcctcg agtccggcgc cgatgggaag
      961 cccgccttcg gagaaggccg tctgtgtgcg accgtaaaca atctgaagaa aaatttccct
     1021 catcgcggtg ttgatcgcat cgcagacaag gtcggaattc agcgcttcca gaatggtttt
     1081 gttcggcaga atctcggcag ccattgcggc cgagtgggtc atgccggtgc aaccgagaca
     1141 ttcgacgagt gcctcttcaa tgatcccgtc tttgacgttc agggtcaact tacatgttcc
     1201 ctgctgcggc gcacaccagc cgacaccatg ggtaagaccg ctgatatcct tgatttcttt
     1261 caccatggtc catttgccct cctgtgggat cggggcggga ccgtttttcg cgcctttggc
     1321 aactacgcac atctgttcaa cttcatgagt gtattccata aactatctct ccttccggta
     1381 ataccagttc gctttcacgg ttcatttcag ccgttgaaag taatttgttt ttataatact
     1441 gccggtcaca atctagcacc ggaggggtta tttttcaaat taatttgttg taaggaagct
     1501 taagtttttg attatattga gatgttaaaa aaaagatttt ctcaagagag aacgaaaagc
     1561 aaagccgacg ccgcaaagcg gcgtcggctt gaatgcgtta tcgggtatga ttggatttgc
     1621 ccctctttct gaatcgcccg gtaatctcct catcgccgcg acatcagcgc aaacactccc
     1681 cagcccaaat attcacgcgt gtaagcggcg tggcgcacgg gctccgaggt cagttgggca
     1741 cgaacctctt tggccaattc gtcgccggga ttggcttcaa gccatcggcg catggtgagc
     1801 catttggccg cctcgtatcg gtcccaaccg tcctgatcag ccagaatcat ttcaacgacg
     1861 tcgtagccga gacggtcgaa agacgcgaga agttccggaa gcatgataaa gtcggcaatc
     1921 gtgtgggcaa gacacccttt ggcaacatct tccgtcggcg gtaactgccg ccagtaaggc
     1981 tcgccgataa ggatgatccc tccgagcttc aggctttgcg ccagaagctc gatcgtgccg
     2041 gcgactcccc cgccgatcca cgtggcccct agacaggctg ccacatcgac cttctcgcca
     2101 gaaacaaagc ccgcagcatc gccgtgaatg aacgtgacct gatcggcgac gccgagttct
     2161 tcagcgcgac gttttgcttg ctcggtgaac aaccggctca tgtcgatgcc ggtgccgatg
     2221 atgccgtgat cgcgtgccca ggtgcacagc atctcccccg aaccgctgcc gaggtcaagt
     2281 acccggtccc ccgattccag acgcagcgcc gagccgagaa tggcgagctt ttcgggtgta
     2341 atcgggttgt ggatgcggtg agcgctctca gtaatgttga agatccgggg aatatccaat
     2401 gcagaaaatc tcctttcggg tgtgaataga ttcggcgtgg gccgaaccat taatatatca
     2461 attttgtttt tccgattctt gaatattaac ctacaccatc tattgataaa agaaaattaa
     2521 aatatccttg aaaatcaatt tcaaataaac ttttttcact tggccttttg tgatagtaac
     2581 atccttacgt cacgttggtt atttattggt aaatatgtcc gaagaacaac tcattatgaa
     2641 aatcttggat agaaaatttt atcatcggga tacgctgcgg gtggcgcggg agcttttggg
     2701 gaagaagctg gtgcggcgga taaacggtct tgaactttcc ggcatgattg cggagacgga
     2761 ggcttactgc ggccgggcag acagcgcctg ccatgcccac cgcggcaaga cacagcgcaa
     2821 tgcggtcatg ttcggcaaac ccggccatgc ctatgtttat tttacctatg gtatgcacta
     2881 catgctcaat ttggtcactg aagaagagga aaatccctgt gccgttttga tccgcgcaat
     2941 tctaccgatt gacggcatcg cggcgatgga agcccgccgc aaaaagaaag gcaccgaatt
     3001 aaccaacgga ccggccaagc tttgccaggc gctgtccatc gacaaatctt taaacggctg
     3061 gaatgtaacg ctgggcagtg aattgtgggt ggaaaataat aaaaaaatcc cggattctaa
     3121 aatcatcacg acaccccgca tcggcatcga ttacgcaaag acaaaagaca gggaggcgct
     3181 ttggcgattc attttgaaga aagacgatct tgaaggaaga cccgtcagat aaaaatcagc
     3241 tttcaatttt ttcctcttgt ttttcctgat aaaactcaag aaataatttc cagctggcca
     3301 ggaaaagagc ggcgatgatc ggtcccatga caaagcccga cagtcctaaa acggccaacc
     3361 cgcccagcgt ggaaaggaat atcaaaagcg cgtgcatgtt cgtatcgcgg cccagcaaaa
     3421 ccggccgcaa taaattatcc acactgctga taatcaccga tccgaaaata aggatgacaa
     3481 tgccctgcca ggtatgcccc aaaaccagca tgatgattcc ggcgggcacc cagacaaagg
     3541 cacatcccac tcccggcaat atggatagcc ccatcatgat cacgccccag atgagagccc
     3601 tctcaattcc cgtaatgtaa aaaacaaggc cgcccaggaa tccctggatg ccgccgatga
     3661 caaacgtaaa tttcagggac gctttggccg tggaaaggaa ctggtaaatg aacgtatcga
     3721 ggtagcgctt attcacggga atatgaccga ccatagattc cagaagtctt tccccgtcgc
     3781 gcaggaaata aaaaagggtg tagatcatca cggcaacttt gacgatgaca agaatggtgt
     3841 tttgcgtaaa attcgaaata tgattgatga cgtatccgct gaccgccttg atgatgtcct
     3901 gaaattttcc cagtataaaa gcctgatcca ggttcaattt ggcaaacaaa gcactctggc
     3961 tcaatgtgtt gagcgttgcc gtaagtgttt tcatccaggc gccgccctgg gaatcgaagg
     4021 acttatagat atcaaacgct tccagcacaa gaaggttaaa cagaaatgcg atagggataa
     4081 tcaaacaaag cagaataaca acgagagtcg cgcccgcgca taaattggga tttgtgattt
     4141 ttgtattcaa cgcccggtgc aggggcataa aaatgccggc gatcagcgcc gcccagaaaa
     4201 ccgcaaagaa aaaaggctta acaatgtaaa agaagaaaag ggtgatgatg acgagtaaaa
     4261 cgataaaacc gatgttttgg aagttataac cgggcttttt gttcatattc atccatcgac
     4321 ctatcatcgc gaaccctgat gcgtctatat tgccatcaga ctgtttttgc tgcggaaggt
     4381 ataaaaacgt aattaataat aaactacagc aacttataat gtcaattcat aaataaccct
     4441 tggcaagaaa aagattttga ttgacatatt gtcaggtatg actattttac atccgagatg
     4501 aataacgact attcatcaaa aaggaggata tttgatgttt acagtgacag agaaggcatt
     4561 ggaggtaatc aaagatttta tcaaagcaaa aaaacctgat tccgctgtgc ggataacaat
     4621 gtctattggc tgatccggcc ccgcactggg gatggctctg gatgagccgg caaaagatga
     4681 tgaagtattt gatgaaaacg gaacaaaatt tgtaattgac aaggacattt ttaatcaagc
     4741 gaaaccgatt aatgttgatt ttatagaaac accgcagggc tctggattta aggtaacgtc
     4801 caacctgtct gccgccggag gcggttgcgg ttcttcctgc agcggctgct agtctttggt
     4861 aaggggagga gacatcctcc ccttttttta acgatgaccg acgaaaaaag cacaaaaaga
     4921 gatctggcgg aaaagctcct tcaaaaaggc aaatatgatg aagccattgt cctgctcgag
     4981 gatattcatc aggaatcacc ggacgaagac cctgtccttt taatgctggc ctgggcatat
     5041 tacgacagcg gcaaaaccgg tcaggccgaa aaatgtttaa cgatattgtt cgaccgcgag
     5101 ctcgagagaa aggtgtttac cggctttgcg ttcgatgaac tggtgcgtct ttacaagcag
     5161 aagaaggact tcccgaatct ggtaaatatt tgcgagaggg cagttgccgc gcagccggac
     5221 gatgttgggc tcctgacgga gttaggcaat gcctatcttc aggcgggaca ggccgatcgg
     5281 gcacgtttaa cgtatgaaaa actgattgcc atggaagacg acaatcccgc tttttattgt
     5341 ttgtggggcg aagcgttgtt tgcggcggga ttaaacaaag aaagcgaaga cgcttaccgg
     5401 aaggccggcc aactggatcc cgatcagctc gacagctact atttcaggat tgccgtttta
     5461 ttccaaaaag ccgataatca tcaggaagcc cggcgtctgt tgaataaatg cctgaacctc
     5521 aatccttcca gtcccctgta ttactgctcg ctcggggaca gtcttatcgg tctgggaaaa
     5581 ttacaggatg cgatcaacgc ttacaatcag gctgtcgaat ttgatcgggc agatgcggat
     5641 gcctactaca accgattagg taactctctg atgaaaaccg gaaatttttc ggaggcggta
     5701 gccgcgtttc aaacagccat acggctcgag ccgctgccca tctattatct gaacctgtct
     5761 tccgcctaca gaaaaatggg gcttgatcac gaggccgatc agacaatcgg caaagccgat
     5821 aaaatccact gaattaaact ctgcccagtt tgcgctgcaa ctccttgaaa acgtgataga
     5881 aatattcgtt ttcttccatt tcttttttca gaatcaggcc tatttccgaa atttcttcca
     5941 tatgaatgat taaattcacg ctcctgttca acgtgttcat gtaatccatc gtggcaaacg
     6001 tcgagctgat caggacaacg aaaacctgcc ggcggacgga catgggaaga gtctccagat
     6061 atttaaggac ctggttgacg ccgtccttgt ccgtgtcata atgttcattg acaacaatca
     6121 cgttgaacgt ctgaaacgtc gtgtacttca gagcttcttt cgccgtcatc ggttcaatca
     6181 cgttaaagtt aagcgtttgc agattttctc tcagcttttg cctgatcgcc ggatcgttct
     6241 cgcagatcat ggccgagctg gtatccatat cccggtagtc aaaaggacgg cccgagtaat
     6301 cgatctcctc ttcgactgat tctggtgcca atttatccgt gttcataatc cattcccgat
     6361 agttatttta ttaagtccgg acattatctt ttccatgtgt ccatttttgc ttttccgtaa
     6421 gaccggtcga cttccagcgt tccgatattg acggcttgat cgccgcgggc tttcttgacg
     6481 gcatcgatac cgcgattgac tacagctctg ttcgtcgcaa acgcaatggc cgtttcctcc
     6541 gtaatcaaat ccttgctgaa atgatcgata atgcagtcgt caaaagtcat catgccgaaa
     6601 ggccttccct gtgtgatgat atcattaaat gtttttcctt ccgattcgcc gtgcagaata
     6661 acatccttca cccgcaggct ggtgctcagt gtttcaaatg cggcaacacg ccccccgcct
     6721 attttcggca gtaaacgctg acaggcgatc cagcggatgg catcggccag ccgcacacgt
     6781 atctgatttt cttcatcccg cgggaacata ccgataatac ggttgatcgt ctgtcccgca
     6841 tccacggtgt gcagtgtact caatacgaga tgaccggttt ccgcggcgct taaagcgatt
     6901 tcgaccgatt cccggtcgcg catttcaccg accagaatca ccttgggagc ctggcgcatc
     6961 gcggcccgta agccgctgga aaatgtatcg aaatccttgc cgagttcccg ctgattgatt
     7021 gtagccttct tctgtggatg cgtgtattcc accgggtctt ccagggtaat aatgtggacg
     7081 gaactggttt cattgatttc gttgatgatc gcggccagtg acgtggtttt accggtgccg
     7141 gtcgctccgg tgactaacac aatgccgttt ttctcgcggg atatcctgta ataggcatcc
     7201 ggcaatccta gatcggcgca ggaaggaaca agggattcca gctttcttaa gacaatcgaa
     7261 taattgccca tttgcgaaaa aatattcacg cgaaaacgcg ccacgccggt caattcatac
     7321 gacaaatcgc atgcgccttc cttaagcaag tccgtcatga gacggtgatc ctgattcacc
     7381 aggttcaaag caaaaatttc cgtctgaaac ggcgtcagtt tctcaaaatg cggcctcagc
     7441 ggaacgggaa ccagctgacc ggcagcctcc acctgaaaag gtttgcccac ggttatgata
     7501 aaatcagaaa tgttgggata cgactccagc attttggtca gaatatgatc aatttccggc
     7561 tttctcatat acgatccttt ctcgatacat tacgcttcgg taaaatcggt cggcggttgt
     7621 ctgagcagcg gacggaaccg ggctttttca ttggctttga tataggcatc atcgccgctt
     7681 atccaaccgc ggttgtacag atccataatg gcatcgtcca gaagctgcat cccgtatttt
     7741 ttaccggtct gcatcatgga gggaatctgg taggtcttgg attcccggat taagttgcgg
     7801 accgccggcg tggcaataca gatttccaga gcgacgcagc ggcttttgac atcaattctc
     7861 ttgaataaaa cctgagaaat aacggcgcgt aaaccatcag ccagtgtcga gcggatctgc
     7921 aactgttcgc tggcgggaaa aacttcgatg attctgtcaa cggttttggg cgcgctcgtc
     7981 gtatgaacgg tggaaaaaac cagatggccg gtggaggccg cttcaatcgc cagagaaatg
     8041 gtttccagat cgcgcatttc tccgaccaga ataatatccg gatcctcgcg taatgctccc
     8101 cgtaatgccg cagtaaaagt tttggtatgc acccccactt cgcggtggtt gacgatgcaa
     8161 ttttggctct tatggacaaa ttcgatcggg tcttcaatgg taatgatatg gtcccggcgc
     8221 aggcggttgg cttcatcaat aatagcggcc agcgttgtgg acttgccgct gccggtcggc
     8281 cctgtcacca gtacaaggcc ccgcggaagc tgggccagtt tggcaattac gttgggcagt
     8341 cccagttgct gacaggtctg aattttatcg ggaatttctc gaaaaaccgc tgccatgccg
     8401 tttttctgcc tgaaaaaatt gacacgataa cgggccagat tgggcacttc ataggcaaag
     8461 tcgacatcgc cggtttcttc gaactgtttg attttgtgtt ccggagtaat ttcataaaga
     8521 agcactttca aggcatcatt ggtaagaaca tcatacttaa tccgctcgat atcgccgcga
     8581 attctgattg ccggctgctg tccggcgaca agatgaaggt cggatgcgcc ctgatcgtgc
     8641 attaaattaa agaaagcgtc aattttggcc atctatttgc tcctcgaggt gatttgattt
     8701 tccttatcat tttttctttg tgaaaagaat atcttatttg aaaatttcac attcatcgat
     8761 aaaaacatat ttcatttttt tatgtgcgta gagtataaga aaaaccctgc tgttttgtca
     8821 attcaataat catagcaact atggcttgac accgcgatca tcaataatat atgcttccgt
     8881 gcaattaccc ggcgggaatt cttaaggttg gggatgtagc tcagctggga gagcgcgacg
     8941 ttcgcaatgt cgaggccagg ggttcgatcc ccctcatctc cacca
//
LOCUS       DAOSME010000077         8453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_9367_length_8453_cov_25.998333, whole genome shotgun sequence.
ACCESSION   DAOSME010000077 DAOSME010000000
VERSION     DAOSME010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9367_length_8453_cov_25.998333"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLG98_09785"
     CDS             complement(<1..684)
                     /locus_tag="PLG98_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33592.1"
                     /translation="MKKYSSVEKMAPVGFVLLGAGSVGGGVFHQSLLTRDVDCKVICD
                     KAMEKVMALEDPRRRFEIVSTEGELADALNAGKVAVCEDAQLAAIAPGVEVFFDASTA
                     VDSASRTIESAMRSGKHVVMMNAEADAVFGPWFWHLAQKYGVAYTSSDGDQPAVIARL
                     VEELRFYGLDFVMAGNIKGFLDRYTDPVKITPEADKRFLDHKMCSSFTDGTKLCVEMS
                     IVANALGCSV"
     gene            1163..2443
                     /locus_tag="PLG98_09790"
     CDS             1163..2443
                     /locus_tag="PLG98_09790"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE33593.1"
                     /translation="MIEFIGYFCASLAIASSLIGLLSVFIFRSHIKHWTRKTPVTYPA
                     LTVISPQRGKIDPENVEAILEQNYPGVWEIIFVTTKDDASLPQLQHYLQKYKNVKIAI
                     AEDVVELATTKGIHRCQKNNNLLTAIKAASPQTEVYAIIDADARPFSDWLSCLVAPLT
                     DANSRLGVVTSARIYLPGKGLASLVQVLWILISAMFLVGKCRYIWGGGLAIPKKVFDN
                     ANLARQINGQGGSSITTDDMNIYNALRKQGYESLFVPDCIVLRHPPKHKESLFNVIGF
                     TNRQVLQTWWTTRGIWVLLCNIGIRLPMLLCALVIAWWYPWCLLALLSIVIDVFMGIS
                     ALKTVIDAEPRLSAKLIFWIMKSKGALAGRSLQPEIRIQVNFWIVLLPIVTPFVVTLN
                     TFTVPFFRKMYWSGIAYTRRKVLGYTGDFSWRTK"
     gene            2677..4179
                     /locus_tag="PLG98_09795"
     CDS             2677..4179
                     /locus_tag="PLG98_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE33594.1"
                     /translation="MSKKQLIELVSSLQAQLASADASSLSGTSGEDLYRFLAQSSQVG
                     FYIMQDGRFRFINSHFRDYAGYTEEEMLKMDPISLILPEDLRKTAKSAVMMLKEQRFS
                     PYEFRIVTRDGRIKWIMETVMSIKFKGRRAILGNSMDVTERKEAGRRLEELEALESSI
                     LDAIPQAVVGLHERRINFANHAVKIIFGWHREELIGKSIRMIYRNEEEADTIGDLFYS
                     TLEKQRTYETEFPCRCKDGREIICRMKASRIGEKLREKRIVVTFEDITAQKKAQEDLE
                     RSRQDLRNLSAHLQSVREEESTRMARKIHDELGQSLTALQMDLSWLKTRLPTSNNSIR
                     EKTKSMSELVDATIESVHTITTELRPSLLDDLGLPAAIEWQAGDFQKRSGIRCQAYIT
                     GNMETISKDLATSIFRIFQETLTNIARHSKATQCKVTLSERGKELCLEVTDNGIGITQ
                     WQIDDSRSFGIIGMRERAHLWDGTVHVRNAKPSGTTVRVLIPLGKGEKLS"
     gene            4176..4808
                     /locus_tag="PLG98_09800"
     CDS             4176..4808
                     /locus_tag="PLG98_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE33595.1"
                     /translation="MIRILVADDHAVVRQGVKQILADVTDMTVQDEAQNGPETLRKVT
                     DNNYDVVLLDISMPGRSGLEVLEDIKAQHPKLAVLILSMHPEEQYAVRALRAGASGYL
                     TKASAPQELIGAIRKVAGGGKYVTSSLAEKLADELEIDTEKLPHERLSNREHQVMLML
                     AEGKSVSDIADELCLSVKTISTYRTRVMSKMGMKKNAELTLYAVHNKLIG"
     gene            5017..7698
                     /locus_tag="PLG98_09805"
     CDS             5017..7698
                     /locus_tag="PLG98_09805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE33596.1"
                     /translation="MNQEFSTSENAQSTEKNVQSDSSFARALLKDELAEIIHQAVRDN
                     RSYLMEHEAKEILEGIGIRTTGFLVARSEDEVLAMSEKIGYPVVLKIVSPDVVHKTDA
                     GGVKLNLHSAGDVRIAYQDILEKFKYQHIEGVTVQKMAKPGIEAIIGVTRDPSFGPLI
                     MFGLGGVFVEVLRDVSFRILPITEKDASEMIAEIRGAAILEGYRGQAVDLDSLSNLLM
                     KISQLVIENPEISELDLNPLFLYPDGYITVDARMFVKETSPGENVTATGRESLRDFFY
                     PKSIAVIGASDVKGKLGYNVFWNLINHDFPGMLYPINPKKDSIKGVKCYKSILDVPDP
                     VDMAIVIVPAKAVEAAISDCCSKGVKYVVVEAAGFAETGEEGRQAQERIEKIIRAHGC
                     RVLGPNCSGVINTHHNMVQSIGLLSDLHKGNVGMVAQAGVYAAGILTGLSNVLNFGIV
                     ATIGNKMDINETDILEYLSDDDHISVIVMYMEDIRSGKRFVDVASRAAVRKPVIILKS
                     GRTEAGKKAVSSHTASLAGNDEINSAAFKQSGIIRARDNEHLFALTRAFSKQPVPKGN
                     GVLVITYTGSMGVAATDMLYLSNMRLAALEPYFQKRLRQVMPDYASIGNPIDCSFSMT
                     PRQVKDLIDIGVESGDVHGFIVIIQGEILGSFVDVMKNIDYKEKPVLCCVACKEFMID
                     DVVKMEQAGIPVYSTAEMAAEVLSQMYHYGVRRQSAIADSIARHITRNSLSVDNRAVR
                     LRLINPKDIGLWTDFVNNCSEKSLWLRFLSPFSATPERAQRFCNVNPEEEIAVVAEMK
                     DGDKYKFLGIARLIKNKRCEGEAEYAIIVSDNWQNKSLGHTLSEQCIELARKEGYKTI
                     RAETLQENYAMLRIFRHCDFVLDSKDENMVSMTLNLV"
     gene            7917..>8453
                     /locus_tag="PLG98_09810"
     CDS             7917..>8453
                     /locus_tag="PLG98_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOE33597.1"
                     /translation="MEERRKSPRIKDENEVTITVFSEENNIPKEKIIENFTKDISAGG
                     AKLQTNILMPVDTLIELEFTPKGLRQQINAIGKVKWVKVLIDDESYQAGVEFSGPPNE
                     AVSKLQDYISWKLKSHKVDYIKEKLSPVDSGDVNIEKKPEIAPIDPAAIEITKDDAIP
                     RDQSGQKDTEKIKREPRAR"
BASE COUNT         2270 a         2106 c         2107 g         1970 t
ORIGIN      
        1 aacagagcat ccgagggcat tggcaacgat tgacatttcc acacagagct ttgttccatc
       61 cgtaaatgaa gaacacattt tatgatcaag aaatcgttta tcggcttcag gagtgatttt
      121 tacgggatcg gtgtaacgat caaggaaccc tttgatgttg ccggccatca caaagtcgag
      181 accgtaaaac cgcaattctt ccactaaacg cgcaatcacg gcaggttgat caccgtcgct
      241 gctcgtatag gcaacgccgt atttctgtgc cagatgccag aaccaggggc cgaaaacagc
      301 gtccgcttcg gcattcatca taacaacgtg tttccccgaa cgcatggcgg attcgatggt
      361 ccgggaggcg gagtccacag ctgtcgaagc atcaaaaaaa acttcaacac cgggggcaat
      421 agccgccagt tgagcatcct cgcaaacggc aacctttccc gcgtttaaag catcggcaag
      481 ttctccttcg gttgagacga tctcgaagcg ccgtcgagga tcctccaaag ccatcacctt
      541 ctccatcgct ttatcgcaaa tgactttgca atccacatcc cgggtcagca aagattgatg
      601 gaaaacccct ccgccaaccg aaccggctcc cagaagaaca aaaccgacag gggccatctt
      661 ttcgacgctt gaatattttt tcatagaaga cctcaaaaaa acgaaatgtc tttccccggg
      721 gaaaatatac ttgaattgcg gatgcgcgtc aatggattac caaccttact gtcgcgggat
      781 gtaaatattt ttcccaatgc cctcaaggct gcctcatgtt tgaaataaaa acggtccctg
      841 accaattttt aaagttgcag atagatccgg atgcaaccat caccccggca gaaagcttcg
      901 tcactcgtgc tccgatgtgg cttttcggta ccgtatccgg atgtcaatga cctgttaaaa
      961 ttccaatgaa tattaccttg acagacaggt cagcatcgat tatgttcccg cagtactttt
     1021 ttaacattag taaaatggat atagacatca tgcaggatga aggccgtctt acggcgaaaa
     1081 ggtttttttc atttcaccgg acgatattgc atcactcttt ctcctcgctg caatccatga
     1141 gctgaatgga tgtggacgtt ctgtgattga attcattgga tatttttgcg catctctggc
     1201 gattgccagc tcgctgatcg gattactctc tgtttttatc ttccgctcgc atatcaagca
     1261 ctggaccaga aagacgccgg taacgtatcc ggcgctgacg gtgatttctc cgcaacgggg
     1321 caaaatcgac ccggaaaatg tcgaggctat cctggaacaa aattatcccg gcgtctggga
     1381 aattattttt gtcacgacaa aagacgatgc ttccctgccg cagttacaac actatttaca
     1441 aaaatataaa aatgtaaaaa tagccatcgc cgaagacgtg gttgaactgg caacaacaaa
     1501 agggattcac cggtgtcaga agaacaataa tttattaact gcgattaaag ccgcctctcc
     1561 gcaaacagaa gtttatgcta ttattgatgc cgatgcccgt cccttcagtg actggctaag
     1621 ctgtctcgtc gctcctctca ctgacgccaa ttccaggctg ggggttgtta cgtccgcaag
     1681 aatttacctt ccggggaaag ggctggcctc tctggttcaa gttttatgga ttttgatttc
     1741 tgccatgttt cttgtgggaa agtgccgcta tatatgggga ggcggactgg ccattcccaa
     1801 aaaagtattt gataatgcaa acctggcgcg acagatcaat ggacaggggg gaagttccat
     1861 caccaccgac gacatgaata tatataatgc cttgcggaaa cagggatacg agtctctgtt
     1921 cgtacctgac tgtatcgttc tcagacatcc tccgaagcat aaggaaagtt tattcaacgt
     1981 catcggattt accaaccggc aggtcttgca gacctggtgg acgaccaggg gcatatgggt
     2041 tttgctgtgc aatatcggca tcaggctgcc gatgctttta tgcgcgctgg tcatagcctg
     2101 gtggtatccc tggtgtctgc tggcgttatt atccattgtc attgacgtct tcatgggaat
     2161 ctccgcgctg aaaaccgtca tcgacgcaga accgagatta agcgccaaat tgattttctg
     2221 gatcatgaag tcaaaaggag ccctcgccgg ccgctcactg caaccggaaa taagaatcca
     2281 agtcaatttc tggatcgttc tgcttcccat tgtcacgccg tttgtcgtga cccttaacac
     2341 ctttaccgta ccttttttca ggaaaatgta ctggagcgga atcgcatata caagacggaa
     2401 agtcttggga tataccggcg atttttcctg gcggaccaag tgacgccaaa tcgatttttg
     2461 atattgcttt caagttgttt tccatccccc ggttctcttc acgggttatt tcgatttttt
     2521 tgaatcgaca tcctgaaaaa agcgtctcat agatacagct gccatctgtt ctggaaaaac
     2581 cggccatcaa agatatttat ttttgatgtg ttttatgatt aaataatttt ttcatggctg
     2641 agaatagaat cgggtggctc aatgaatccg gaaaagatgt ccaaaaaaca gctgatcgaa
     2701 ctggtgagca gtcttcaggc acaactggct tcagccgacg cgtcgtccct atccggcacg
     2761 tccggagaag atctttatcg attcctcgcc caaagctctc aggtcggatt ttacatcatg
     2821 caggacggcc gttttagatt catcaattca cattttcgcg actacgccgg ttataccgaa
     2881 gaagaaatgc tcaagatgga tccgatttcc ctcatcctcc ccgaggatct ccggaaaaca
     2941 gcaaaaagcg ccgtcatgat gctcaaggaa cagcgctttt ctccttatga attccgtatc
     3001 gtcaccagag acggccggat caagtggatc atggaaacgg tcatgtccat taaattcaag
     3061 ggaagacggg ccattctggg aaattccatg gatgtcaccg aacggaaaga agccggtcgg
     3121 cgcctggaag agctggaagc gctggaatcg tccatcctgg atgccattcc ccaggcggtc
     3181 gtgggacttc atgaacgccg gatcaatttt gccaatcacg cggtaaaaat catttttggc
     3241 tggcaccggg aggaactcat cggcaaaagc atccggatga tttaccgtaa cgaagaagaa
     3301 gccgacacaa tcggcgacct gttttattcc acgctggaaa aacaacgcac atatgaaacc
     3361 gaattcccct gccggtgcaa ggacggccgg gaaattatct gccggatgaa agcctcgcgg
     3421 atcggtgaaa aactgcgcga aaaacgtatc gtggtgacct ttgaagacat taccgcacaa
     3481 aagaaagctc aggaagatct ggaacgctca cggcaggact tgagaaatct ttccgcgcat
     3541 cttcaatcgg tgcgtgaaga agaaagcacg cgcatggcgc gcaaaattca tgatgaactg
     3601 gggcagtcgc tgacggcgtt acagatggat ttatcgtggt tgaaaacccg tttgccgacc
     3661 agcaacaata gtatccggga gaaaacaaag agcatgtcgg aactcgttga cgctaccatt
     3721 gaaagtgtgc acacgatcac cacggaactg cgcccgagtc tgctcgatga tctgggacta
     3781 ccggcagcga tcgaatggca ggctggcgat tttcagaaac gatccgggat tcgctgtcag
     3841 gcgtatatca ccggcaatat ggagaccatc agcaaggatc tggcaacctc catctttcgt
     3901 atttttcagg aaacactcac caatatcgcc cgtcattcca aggcgacaca atgcaaagtc
     3961 accctttcgg agagaggaaa ggaattgtgt ctggaagtta cggacaacgg gatcggcatt
     4021 acgcaatggc aaattgacga ttcccggtct ttcggcatca tcggcatgcg tgaacgcgct
     4081 catctctggg acggcaccgt tcatgtccgc aacgccaaac cgtccggaac aacggtaagg
     4141 gttttaattc cattgggaaa aggagaaaaa ttgtcatgat acgtatactg gtcgcggatg
     4201 atcacgccgt ggtgcgtcag ggtgtcaaac aaattctggc ggacgtcacg gacatgaccg
     4261 ttcaagacga ggctcaaaac ggtcctgaaa ccctgagaaa agtcaccgac aacaactacg
     4321 acgtggttct gctggatatt tccatgccgg gccgcagcgg actggaagtg ctggaagata
     4381 ttaaagccca gcatcccaaa ctggccgttt tgattctaag catgcatccg gaggaacaat
     4441 acgcggttcg ggctctgcgg gccggagcct cgggctacct gaccaaggcc agcgccccgc
     4501 aggaattgat cggcgccatc cgcaaggtgg cgggaggcgg aaaatatgtc acgtcctcgc
     4561 tggccgaaaa actggccgac gagctggaaa tcgacacgga aaaattgcca catgaacgtc
     4621 tgtccaaccg cgagcaccag gttatgctca tgctggcgga aggaaagtcc gtttccgata
     4681 tcgccgacga attatgtctg agtgttaaaa ctatcagtac ctaccggacg cgcgtcatgt
     4741 ccaaaatggg catgaaaaaa aacgcggagc tcaccctgta tgcggttcac aacaaattaa
     4801 tcggttaggt gtttacgttt ctgctttttt ccatgcccgc gctgcacgtt tgtcggacaa
     4861 aatccgtcag gcgattgcgc actgttccga caaacaaaca agctttccgc cgatattacc
     4921 ttttcaatct tcatgctaat ccgttaccat gttcacgttt ggcattaacg tgtaccgtaa
     4981 agagaaaacg tcggataatt gcagggaggc gggggcatga atcaggaatt ttcgacatca
     5041 gaaaacgcgc aatcaacaga aaaaaatgtt cagagtgatt catctttcgc aagagcactt
     5101 ctgaaagatg aattggctga aatcattcat caggccgtca gggataaccg cagctatctg
     5161 atggaacatg aagccaagga aatcctcgaa ggcatcggca tccggaccac cggctttctg
     5221 gtggcgcgtt ccgaagatga agtgctggcc atgagcgaga agataggcta ccccgttgtt
     5281 ctgaaaattg tttcacccga tgtggttcac aaaaccgacg cgggcggcgt caaactgaac
     5341 ctgcattccg cgggagatgt gcgcatagcg tatcaggaca ttcttgaaaa attcaagtat
     5401 cagcatatcg aaggcgttac ggttcagaaa atggccaagc cgggcatcga agccatcatc
     5461 ggcgtgacgc gcgatcccag cttcggtccg ctgatcatgt tcggcctcgg cggcgtattt
     5521 gtagaagtgc tgcgcgatgt atctttccgg attctgccga tcacggaaaa agatgcttcc
     5581 gaaatgatcg cggaaattcg gggggccgcc attctggaag gctatcgcgg ccaggcagtc
     5641 gatctggact ccctcagcaa tctgctgatg aagatatcgc aactggtcat cgaaaatccg
     5701 gagatcagcg aactggacct taatccgctg tttttatatc ctgacggcta tattaccgtc
     5761 gacgcccgaa tgtttgtcaa ggaaacatcc cccggggaaa atgttaccgc gacaggccgg
     5821 gaaagcctgc gggatttctt ttatccgaag agcatcgccg tgatcggcgc atccgacgtc
     5881 aaaggaaaac tgggctataa cgtgttctgg aatctcatca atcatgattt ccccggcatg
     5941 ctttatccga tcaatccgaa aaaagattcc atcaagggcg tgaaatgcta caaatcgatt
     6001 ctggatgtgc ccgatccggt ggacatggct attgtcattg ttccggccaa ggcggtggag
     6061 gcggccattt ccgattgctg ctcaaagggt gtaaagtacg tcgtggtgga ggcggcagga
     6121 ttcgccgaaa ccggcgagga gggaagacag gcgcaggaga gaatcgagaa aatcatccgc
     6181 gcccacggat gccgggttct gggaccgaac tgttccggcg tcatcaacac gcatcacaac
     6241 atggtgcagt ccatcgggct gctttccgat ttgcacaaag gaaatgtcgg gatggttgcc
     6301 caggccggcg tttacgcggc cggaattctg acaggattga gcaacgtact gaatttcggc
     6361 attgtcgcga ccatcggcaa caagatggat atcaacgaaa cagatattct ggaatatctg
     6421 agtgatgatg atcacatttc cgtgattgtc atgtacatgg aagacatccg cagcggaaaa
     6481 cgttttgtcg atgtggcttc acgggcggcc gtgcgcaagc cggtgatcat cttgaaatcg
     6541 ggacggacgg aagccggtaa aaaagccgtg tcttcccata cagcctcgct cgccggcaac
     6601 gacgagatca acagcgccgc attcaagcag agcggcatca ttcgtgcccg cgataacgag
     6661 catttattcg cactgacccg cgccttctcc aaacagccgg taccgaaggg gaacggcgtc
     6721 ctcgttatta cttataccgg ttcgatgggc gttgccgcga cggacatgct gtacctttcc
     6781 aacatgcgtc tggccgctct ggaaccgtat tttcagaaac gtctcaggca ggtcatgccc
     6841 gattatgcca gtatcggcaa tcccattgac tgttcgtttt ccatgacgcc ccggcaggtg
     6901 aaggacctga tcgacatcgg cgtggaaagc ggcgatgtcc acggttttat cgtgatcatt
     6961 cagggggaga tactcggctc ttttgtggat gtcatgaaaa acatcgatta caaggagaag
     7021 ccggttctct gctgcgtggc ctgcaaggaa ttcatgatcg atgatgtggt caagatggag
     7081 caggccggta ttcccgttta ttccacagcg gaaatggctg cggaggtgct cagccagatg
     7141 taccattacg gtgtcagacg ccagagtgca attgccgatt ccatcgcccg tcacattacc
     7201 agaaattcac tttccgtgga caaccgcgcg gtaaggctgc gcctgatcaa ccccaaggac
     7261 atcggcctct ggaccgattt tgtcaacaat tgttcggaaa agtccctgtg gctgagattc
     7321 ctgtcgccct tttccgccac acccgaacgc gctcagcgtt tctgcaatgt gaatcccgaa
     7381 gaggaaatcg ccgtcgtcgc ggagatgaag gacggcgaca agtacaaatt tctcggcatt
     7441 gcgcgtctga tcaaaaacaa gcgctgtgaa ggcgaagcgg aatatgccat catcgtctcc
     7501 gacaactggc aaaacaaatc cctggggcat actctctccg agcagtgcat tgaattggcc
     7561 cggaaagaag ggtacaagac catccgtgcg gaaacgcttc aggaaaatta cgccatgctg
     7621 agaattttcc gccattgtga ttttgtactc gattccaaag acgaaaatat ggtttccatg
     7681 acgttaaatt tagtctaaat tcaccccata gtttgcagct catacacata acggaacggg
     7741 aacaatgacc tttaccgtgc cgccccgatg ctgttttcct cccccatatc cccttaggca
     7801 tgaaacattg tgccgtcaat cttttattga cagacaaaaa tgatttcaac tatattgagc
     7861 cataggtcat cagattcggg atagaagtaa aatccggcat tcaaaggagc gaatgaatgg
     7921 aagaaagacg aaaatcgccc aggatcaaag acgaaaacga ggtcaccata accgtttttt
     7981 ccgaagagaa taatattccg aaagaaaaaa tcattgaaaa tttcaccaag gacatctcag
     8041 cgggcggcgc caagcttcag accaatattc tgatgccggt agatacgctt atcgaactgg
     8101 agtttacgcc caaagggttg cggcagcaga tcaatgccat cggaaaagtc aaatgggtga
     8161 aggtgctgat tgacgatgag tcgtatcaag ccggtgtgga attttcaggc ccgcccaatg
     8221 aagccgtcag caaacttcag gattacatct catggaaatt aaaatcgcac aaggtggact
     8281 acattaaaga aaaactttct cctgttgatt ccggcgatgt taatatcgaa aaaaaaccgg
     8341 agattgcccc gatcgatccc gctgcgatag aaatcacaaa agacgatgca attccccggg
     8401 atcagtccgg acagaaagac actgaaaaaa tcaaacgaga acctcgcgca agg
//
LOCUS       DAOSME010000078         8298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_9617_length_8298_cov_24.011646, whole genome shotgun sequence.
ACCESSION   DAOSME010000078 DAOSME010000000
VERSION     DAOSME010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9617_length_8298_cov_24.011646"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLG98_09815"
     CDS             complement(<1..625)
                     /locus_tag="PLG98_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0F1 subunit beta"
                     /protein_id="HOE33598.1"
                     /translation="MESEQINQGKVLSVRSSVVDVYFSKVPPIRNVLKAGEKEDVIIE
                     VFTQLDNNTVRGVALTPTQGLARGSKVIDTGRPLEVPVGKELMGRVFNVFGQTIDKKG
                     NVEDVEIRSIHREPIPLRDQSTVSEIFETGIKAIDVLAPLERGGKAGLFGGAGVGKTV
                     LITEMIHNTVSAHEGMSIFCGIGERCREGEELYREMEESGVLGNTVMV"
     gene            complement(656..1492)
                     /locus_tag="PLG98_09820"
     CDS             complement(656..1492)
                     /locus_tag="PLG98_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33599.1"
                     /translation="MEAYAAKLIDLIESKADDIARQWAEDVMKHNRTPSYHNLSKPMV
                     IEQGVDFYKLFRRMSMAEHPYEEAKTFSWKYAQKFYDEKIPLQEATYALILLRRNLWL
                     YAEFQGMFFTALEKQQAVESLNRTILMFDYVAYQVIQKYQERIHQDIDTRLFAVETLM
                     KKSCIGGEKKTYQYALMALFVVIAFVITFYYHSVAQTGVIFTHLFYIPIVLASICWGR
                     KGVWVSVFLAVMLLCSHAVFLEGVSFTEDIIRAVMFVVVGGVVGWLMEDVKKVRELFN
                     KR"
     gene            complement(1560..2030)
                     /locus_tag="PLG98_09825"
     CDS             complement(1560..2030)
                     /locus_tag="PLG98_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33600.1"
                     /translation="MKAFTAKLVDLTQTNADEIARQWARDVKKNAKTPSYHQAPEEKI
                     IRQAKEFYNNFQMMFFNESPYEQAKDFFDKYAEERYKEEIPLHEALYALILMRRHMWL
                     YAEFQSLFNVEVDHRQAVESLSRSMLMFDYIMYVVSMKFSKMMQQEVLSRSVKR"
     gene            complement(2380..3747)
                     /locus_tag="PLG98_09830"
     CDS             complement(2380..3747)
                     /locus_tag="PLG98_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE33601.1"
                     /translation="MRILIVDDEEVALNSIKRLLKWRGIRNVDTCANGKDAIAKIKET
                     DFDIVLLDLLMPEVDGLQVLEAAKPYRPHTEFIILTAIDDIPTTVKAIRLGAYDYLVK
                     PVDNDLLFLAIRRAYEHRGLLIGLSTTAGYDKSSIPAAFAEIVTQCPRMCSLLSYAQI
                     MAGGGNPIMITGESGTGKELIARGIHRAGPMPGGPFIAVNVSAIPASMFESQFFGHAK
                     GAFTGADTPHRGYFEQADGGTLFLDEIGELPVGLQSKLLRVLEEKSFYPLGASKPVWV
                     DVRVVSASNRDVEKACQDGSFRLDLFYRLKSVHIHLPPLREREGDIPLLALHFLRKSR
                     ENYKKTITGFNPEAMELLTARNYPGNIRELAQVVENAVLLADSDFIQPCHLGMVSTAP
                     LPLSRQLGTLKENEESHLVYVLKNVQGDRGRAAQILGITVRQLQRKIAAMKKNPQWET
                     ILNDI"
     gene            complement(3744..5885)
                     /locus_tag="PLG98_09835"
     CDS             complement(3744..5885)
                     /locus_tag="PLG98_09835"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE33602.1"
                     /translation="MKTGSSPSRKNKSPGRHESVWTGEVFSNGPQLLQSIIGSSPIPA
                     FIIGKDHLIIYWNRALEELSGIKSKEVMGTGQQWRAFYKTARPCMADLLVDKYQKKIP
                     QWYSGKFIKSKLIDGAYEATDFFPELGKRGKWLRFTSAVIRNSKGSVIGAIETLEDIT
                     QAKRAEEALLKAHEDLEIKVRERTRKLTKANKALKEAMDHLSLILEFLPIVSYTCNAD
                     ESMAITFVSNRVKEITGYTPAQFTANHTFWRNHIHPEDQSNVLKKLKPTARKKTYQLE
                     YRLQIADGSYRWISDHRRLIKPGKHSRSYMVGAWQDITEDKRIRHEGEFRLQQMIQSH
                     KLKALGEVVAGVAHEINNPVSFIANNITMLEEMWNAVEPILASDGASHPNWSEKGIGY
                     VEVCTNMKEIIEEFKTASQRIKRVISGLKEFARTDETVEKKPVKIEEVIQGVMIIVGA
                     QVRKTVSRIDLYIDSNLPPVQGHFQKIEQVIANLLINAHQAITPERKGRIVITARGLD
                     RLNAILIEIEDNGIGMKKAVLDHIFQPFFTTRRDMEGTGLGLSISYGLIKEHNGLIGV
                     LSKPGVGSRFSIYLPLDARTPISIRPAVLCCDSDTAYLKEIKMSIVNVIDWQCGEQDD
                     CDDIIAYLEMHPEVDIFISEINLPVMNGWDLSRKIKERFPLLCVILYSSDPQAGKPEG
                     RMSGKPDYVLQKPFSMTQLQTIIRETGRQRL"
     gene            complement(6100..6723)
                     /gene="yrbL"
                     /locus_tag="PLG98_09840"
     CDS             complement(6100..6723)
                     /gene="yrbL"
                     /locus_tag="PLG98_09840"
                     /inference="COORDINATES: protein motif:HMM:NF007671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HOE33603.1"
                     /translation="MLYIDRTTLIGRGRHRECYKHPEDQNLCIKITVKDSPLEIKREK
                     KYYRHLQKHGISWEMISRYHGDVETDLGQGAVFDLIMDHDGTVAKSLEYYLSSDEQTE
                     KYFSSLSDALCRLKKYLLQNRIVTMDIKPYNILCQMDASGIARLVLIDNIYNSEFIPV
                     SNYCRFFAKRKIDRKWQRFEDSLLNAFKGNKALARMLKERSARRSSR"
     gene            complement(6841..7581)
                     /locus_tag="PLG98_09845"
     CDS             complement(6841..7581)
                     /locus_tag="PLG98_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33604.1"
                     /translation="MKKLFLSILVIILTISFMSWVIYLNQVPAIKQASDSKDIVIEPS
                     PRSDDETAQRALLPDEKSKEISADQGAPATGKSVPAKDRAQVVPKSTAKKPAVSAVDE
                     TKKAPPVSGETLKKPQPAPSEKIQATPASEKSQKEMSRLQDQCEKRSKKIFRKAYGNG
                     VVEKNQNVYLYLYKSHYNKKRDQCFMLVTEDGVLERYKKLLDVDENESLGSVRLNNER
                     KNLGCYLLNSQCDSEETWDLLAKPYMEE"
     gene            complement(7940..8173)
                     /locus_tag="PLG98_09850"
     CDS             complement(7940..8173)
                     /locus_tag="PLG98_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOE33605.1"
                     /translation="MKDKKTYAVVLFKAVSYALKAEKILKQEGLPHKLIPVPKQISSD
                     CGVCLRFEQNIQNQIEAALLNKVEIEDIITLDH"
BASE COUNT         2035 a         2049 c         1863 g         2351 t
ORIGIN      
        1 agaccatcac ggtattgccc aaaacgccgc tttcttccat ttcacggtaa agctcttcac
       61 cctcgcggca ccgctctccg ataccgcaga agatgctcat gccttcatgc gcgctgacgg
      121 tattatgaat catctccgta atcaaaaccg tcttaccgac accggcgccg ccgaacagac
      181 cggctttacc gcctcgttcc agaggagcca gaacatcgat ggctttaatg cccgtttcaa
      241 aaatttccga aacggtggat tgatcgcgca gaggaatcgg ctcacggtga atggaacgta
      301 tttctacgtc ttcaacattc cctttcttat cgattgtctg tccgaaaacg ttgaaaaccc
      361 ttcccataag ctctttgccc acggggactt caaggggcct gcccgtgtca atgacttttg
      421 aacctctggc cagaccctgc gtgggagtca gggcgacgcc ccggaccgta ttgttgtcga
      481 gctgggtaaa gacctcgatg atgacgtcct ctttttcgcc ggctttgaga acattacgaa
      541 tcgggggcac cttggaaaaa taaacatcaa caacactgct gcggacggat aaaacttttc
      601 cctgattgat ctgttcgctt tccacgttca tctcctctta aataaaattg atgctttaac
      661 gtttattgaa aagttctctt acttttttca catcttccat caaccatccc accacgccgc
      721 cgaccacaac aaacattacg gcccgaatga tgtcctccgt aaacgaaacg ccttccagaa
      781 aaacagcgtg actgcaaaga agcatgacgg cgagaaacac agacacccag acaccctttc
      841 tgccccagca aatagacgcc aggacaatgg gaatgtagaa cagatgggta aaaataactc
      901 cggtttgagc cacagagtgg tagtagaatg tgatgacgaa agcgatgacg acaaaaaggg
      961 ccatcagcgc gtactgatag gttttctttt cgcccccgat gcagctcttt ttcatcagcg
     1021 tttccacggc gaaaagtctt gtgtctatgt cctgatgaat gcgttcctga tatttctgta
     1081 tgacctgata ggccacataa tcaaacatca gaatggttct attcaggctc tcgacggcct
     1141 gttgtttctc cagagcagtg aaaaacattc cctgaaattc agcatacagc caaaggttcc
     1201 tgcgcaagag gatcagggcg taagtcgctt cctgaagggg aatcttttca tcgtaaaatt
     1261 tctgggcgta tttccacgaa aatgttttgg cttcttcgta ggggtgttcc gccatggaca
     1321 tgcggcggaa cagtttgtag aaatcgactc cctgttcaat caccatcggt ttcgacaggt
     1381 tatggtagga tggggtccgg ttgtgtttca tcacatcctc ggcccattgc cgcgcaatat
     1441 cgtcggcttt gctttcaatc agatcaataa gtttggcggc gtaagcttcc atggcttcat
     1501 ctcttccaat acaaaccggc ttgataaatc atcgactgcg tcctgctgat gtctgctttt
     1561 cagcgtttca ctgatcttga caaaacctcc tgctgcatca tttttgaaaa tttcatagaa
     1621 acaacgtaca taatgtaatc gaacatcagc atcgatctgc ttaagctttc cacggcctgc
     1681 cgatgatcca cctccacatt gaacagggac tgaaattccg catagagcca catgtgtctt
     1741 ctcatcagaa tcaacgcgta cagggcctcg tgaagcggga tttcttcttt gtacctttcc
     1801 tccgcatact tatcgaaaaa atcttttgcc tgttcataag gagactcgtt aaaaaacatc
     1861 atttgaaaat tattatagaa ttccttagcc tgacgaataa tcttctcttc cggcgcctga
     1921 tggtagctgg gagttttcgc attctttttt acgtctctgg cccactgcct ggctatttcg
     1981 tccgcgtttg tttgagttaa atccacaagc tttgcggtaa aagctttcat tggtcatcat
     2041 ccttttcaaa agaataaaca gcaacgggca tgccaaaact attttcccct cttgatttat
     2101 tcgtgaccgg cttgtctatc cggacaaaaa gcgagacgaa aatcatcaga aacctgtgct
     2161 attgaagcct tacgaaaaag cggatggaaa aatctttccg gtaaaaaaat attttttctt
     2221 tgtcatgtca gcggatcagg tttttgaacg gacgagaaaa aaacgaaata aaagacggcg
     2281 gaaaggatat cgatggacag gccggggact ctttaaaacg gcaagacctt ttaaaaacag
     2341 gaacttattc aaaaagacat ttatgtcgcc cctgacgact taaatgtcat tcaggatggt
     2401 ttcccattgc ggatttttct tcatcgcggc aatttttctt tgcagttggc ggacggttat
     2461 gcccaggatt tgcgccgccc taccgcggtc gccctgaaca tttttcaaaa cgtacaccag
     2521 atgggattct tcgttctcct tgagcgtacc cagttgccgg gataaaggga gcggtgcggt
     2581 ggaaaccatt cccagatgac acggctggat gaaatcggaa tccgccagca aaacggcgtt
     2641 ttcaacaacc tgcgccagtt ccctgatatt gcccggataa tttctggcgg ttaacagttc
     2701 catggcttcc ggattaaagc ctgtaatggt ttttttataa ttctcacgcg atttgcgcag
     2761 aaaatggagc gcgagcagag ggatgtctcc ctctctttct ctcaacggcg gcaaatggat
     2821 gtgcacggat ttcaaacggt aaaaaagatc cagtcggaaa cttccgtcct ggcacgcctt
     2881 ctcgacatct cggttggaag cggagacaac ccggacatcc acccagaccg gcttggaagc
     2941 gcccaaggga tagaaagact tttcttccag tacccgcagc aatttggact gcaggccaac
     3001 gggcagttcc ccgatttcat ccagaaaaag ggtccctccg tccgcctgtt cgaaatagcc
     3061 gcgatgggga gtgtccgcgc cggtgaatgc gcctttggca tggccgaaga actggctttc
     3121 gaacatgctg gcgggaatcg cgctgacgtt cacggcaata aaaggaccgc cgggcatcgg
     3181 ccccgcccga tgaatccctc tggcgatcag ctcctttcct gtcccggact cgccggtaat
     3241 catgatcggg ttgccgcctc cggccatgat ctgtgcatag gaaagaagcg aacacatcct
     3301 cggacactgg gtcacaatct ccgcaaatgc ggcgggaatg ctgcttttat cgtaacctgc
     3361 cgtggtggat aagccgatga gcagcccccg gtgttcgtag gcccgtcgga tggccagaaa
     3421 aagcaagtca ttatccaccg gtttgaccag ataatcatac gcaccgagac gaattgcttt
     3481 caccgtggtg ggaatatcat cgatagcggt aagaataatg aattccgtat gcggccgata
     3541 gggttttgcc gcctccagga cctgcaaacc gtcaacttcc ggcatcagca gatccagaag
     3601 aacaatatcg aaatcggttt ccttgatttt cgcaatcgcg tctttgccgt ttgcacaggt
     3661 gtccacattt ctgatgcccc gccacttcaa aagacgtttg attgaattca gagcaacctc
     3721 ttcatcatct acaatcagta ttctcataat cgttgcctgc ctgtttcacg tatgatcgtt
     3781 tgcaattgcg tcatgctgaa gggtttttgg agcacataat ccggttttcc cgacatgcgt
     3841 ccctcgggtt tgcctgcctg cggatcggag gaataaagaa tgacacagag cagggggaaa
     3901 cgctctttga tttttcggga caaatcccat ccattcatga ccggcagatt aatttccgaa
     3961 ataaaaatgt caacttcggg atgcatttcc agataagcga tgatatcatc gcaatcatcc
     4021 tgctcgccgc actgccagtc aatgacattc acaatgctca tttttatttc tttcaaatag
     4081 gcggtatcgc tgtcgcagca gagaacggcc gggcgaatgc tgatcggtgt gcgggcatcc
     4141 agcggaaggt agattgaaaa acggcttccc actcccggct tggacagcac tccgatcaat
     4201 ccgttatgct ccttgatcag accgtaggag atggaaagcc ccagtccggt cccctccata
     4261 tcccggcgcg tggtaaaaaa gggctggaaa atatgatcca gcaccgcctt tttcatgccg
     4321 atgccgttat cttcaatttc aatcagaatc gcgttgagcc gatcaagacc tctggcggta
     4381 ataacaattc ttccttttcg ttccggtgta atggcctgat gcgcattgat cagcaggttg
     4441 gcgatcacct gttcgatttt ctggaaatgg ccctggacag gcggtaaatt gctgtcgatg
     4501 taaagatcga tgcgggaaac ggttttgcga acctgcgcac cgacaataat catcaccccc
     4561 tgaatgactt cttcgatttt caccggtttt ttctccacgg tttcgtcggt gcgggcaaat
     4621 tccttcagac cggaaataac gcgcttgatt ctttgggacg ccgttttgaa ttcctcgatg
     4681 atttccttca tgttcgtaca gacttccaca tagccaatgc ccttttcact ccagttggga
     4741 tgagacgcgc cgtcactggc cagaatcggt tccaccgcgt tccacatttc ctccagcatg
     4801 gtgatgttgt tcgcgatgaa actgaccggg ttgtttattt catgagcaac gcccgcgacc
     4861 acttcgccga gcgctttcaa tttatgggat tgaatcatct gctgtaagcg aaattctcct
     4921 tcatggcgga ttcttttatc ttctgttata tcctgccatg ctcccaccat gtaactgcgc
     4981 gagtgtttgc ccggtttgat caaacggcga tgatccgata tccagcggta agaaccgtca
     5041 gcgatctgta agcggtactc caactggtaa gtttttttcc tggcagtcgg tttcagtttt
     5101 ttcagaacat tgctctggtc ttcgggatga atgtgattcc gccagaaagt atgattggcc
     5161 gtaaattgag cgggcgtgta tccggtgatt tccttgacgc gattactgac aaacgtgatg
     5221 gccatgcttt cgtcggcatt gcatgtgtag gagacgatgg gaagaaattc caggatcagg
     5281 gataaatgat ccatggcttc tttcagagcc ttgttggcct tggtcagttt tctcgtgcgt
     5341 tccctgactt tgatttccag gtcttcatgc gctttcagca gcgcttcttc cgctcgcttt
     5401 gcctgcgtaa tgtcttcaag ggtttctatc gctccgataa ccgatccctt ggaatttcgg
     5461 atgacggcgg aagtaaaacg caaccatttt cctctcttgc ccagttccgg gaaaaagtcc
     5521 gtcgcttcat atgcaccatc aatcaatttt gatttgataa acttaccgga ataccattgg
     5581 ggaattttct tctgatattt atcaaccagc agatccgcca tgcacggcct ggccgtttta
     5641 taaaacgctc gccactgttg tccggtgccc atcacttcct tcgacttgat tccgcttaat
     5701 tcttcgaggg ccctgttcca gtagataatc agatgatctt tcccgatgat aaaggcgggg
     5761 atcggagaac tcccgataat gctctgcagc aattgcggac cattgctgaa tacttcgccg
     5821 gtccagacgc tttcatgacg tccggggctt ttattttttc tggacgggct gcttccggtt
     5881 ttcatcttct gctaccgaaa atacgactga cgcgtgcgtt tgaactatta aatgatgtat
     5941 taactctgct aaagcaatta tccgcggtga aggtgacgat ttgaaaaatg ttcggtatgg
     6001 tttgattata tagaacggga aactgatgtc aagaaaatta aaaacgaaaa acgcattgct
     6061 tttgccgaca atctgaatcg tactgcatgc aacaacgttt cagcgcgacg aacgccgcgc
     6121 cgaccgctcc ttcagcatcc gcgccagggc tttgtttcct ttgaaggcgt tcaggaggct
     6181 gtcttcaaaa cgctgccatt ttctgtctat tttacgtttt gcaaaaaacc ggcagtaatt
     6241 gctgacggga atgaattcgg aattatatat attatcgatc agaaccaatc gggcaatccc
     6301 ggatgcatcc atctggcaaa gaatattgta gggtttgatg tccatggtta cgatgcggtt
     6361 ctgcaggaga tattttttca gccggcataa tgcatcggaa aggctggaaa agtatttttc
     6421 ggtttgttcg tctgaggata aatagtattc caggcttttg gcaacggtcc cgtcatgatc
     6481 cataatcaga tcgaaaacag ccccctgccc cagatcggtt tcgacatctc catgataccg
     6541 ggaaatcatc tcccaggaaa ttccatgttt ttgaagatgc cgataatatt ttttttcccg
     6601 tttaatttcc agagggctgt cttttaccgt gatttttatg cagaggtttt gatcctccgg
     6661 atgtttatag cattcccgat gcctgcccct tccaatcaag gttgtcctgt ctatgtaaag
     6721 cattactcta ccgttatgat ccggatgatc cgttgcctgc cgcttaaaaa tccgcagcaa
     6781 cttaatcgag gggcaggaaa aacaggcaaa aaactcaaat ttataatttc agaaatttta
     6841 ttactcttcc atataaggtt ttgcgagcaa atcccacgtt tcttccgaat cacattggct
     6901 gtttaaaaga taacagccga gattttttct ttcattgttt aaccggacgc ttcccagact
     6961 ttcgttctca tcaacatcga gtaatttttt atatcgttcc aatacgccgt cttccgtgac
     7021 gagcataaaa cattgatccc gttttttatt gtaatggctc ttatataaat acagatagac
     7081 attttgattt ttctccacga cgccattgcc atacgcttta cgaaatattt ttttgctccg
     7141 tttttcgcat tgatcctgca atctggacat ctctttttga cttttttctg aagctggcgt
     7201 tgcttgtatc ttttccgacg gggccggttg aggttttttg agcgtctctc cggaaaccgg
     7261 cggggctttt tttgtctcat caacggccga aaccgcaggt ttttttgccg ttgattttgg
     7321 tacgacctgt gccctgtctt tggcaggcac cgattttccc gttgccgggg caccctgatc
     7381 agccgaaatt tcttttgact tttcatcagg cagcagcgcc cgttgtgctg tttcatcgtc
     7441 agaacgcggc gatggttcta tgacgatatc ctttgagtcc gacgcttgtt ttatcgcggg
     7501 gacttgattc aaatagatga cccacgacat aaacgatatc gtcagaataa taacaagaat
     7561 ggacagaaaa agttttttca cggcgttttc ctctttcaga tcataaggtt aaaaatttaa
     7621 aaccaacaat tggtcaggat tatatttgtg atattttaat ttttacactg aacgaacaat
     7681 ttaggagttg tgtacatatc agattttcac tgattttcaa aataaatttt atcatttttc
     7741 aaaaacggcc gcgcttcccg tacagaataa gattcacgat gccttcggcg gcaatcattt
     7801 ttacccggaa agtaaattca ttgagggaga agcggacttt aaggatgctt ccggacgaga
     7861 tgaagttatt gttccataag cagacaatct ttcccgcatc cggagcacga acatatattt
     7921 tttcgttatc ggctgatggc taatgatcca gtgtgatgat gtcctctatt tccactttgt
     7981 tgagaagtgc ggcttcgatt tgattctgaa tgttttgttc aaaccgcagg cagactccgc
     8041 agtccgagct gatctgcttg ggcacgggaa tcaatttgtg cggcaagcct tcctgcttga
     8101 gaattttttc cgccttcaag gcatagctca ccgctttaaa cagaacaacg gcatatgttt
     8161 ttttgtcttt catggtgtca gcaatcgtcc ggcgccactc atggtttcca caatatcgta
     8221 catgttggaa acggttccca cggccagctt atccttgatt tcaaaataat tgaggcaggt
     8281 gccgcaaacc agaatctg
//
LOCUS       DAOSME010000079         8050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_9998_length_8050_cov_24.380238, whole genome shotgun sequence.
ACCESSION   DAOSME010000079 DAOSME010000000
VERSION     DAOSME010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9998_length_8050_cov_24.380238"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>149
                     /locus_tag="PLG98_09855"
     CDS             51..>149
                     /locus_tag="PLG98_09855"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33606.1"
                     /translation="MKGKKGLTLTGQLGDVMKESVAAALSFLRANAC"
     assembly_gap    150..223
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <224..520
                     /locus_tag="PLG98_09860"
     CDS             <224..520
                     /locus_tag="PLG98_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33607.1"
                     /translation="GIKEKVLAAYRAGIKTLILPAWNRKDLDDIPANVQKKVKFHFVS
                     DMMEVLRLALEPAKEKTVKQKAGKKAAKSGGAKPAAKRGKSKTKIARKPIAKKK"
     gene            complement(603..2396)
                     /locus_tag="PLG98_09865"
     CDS             complement(603..2396)
                     /locus_tag="PLG98_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE33608.1"
                     /translation="MDPNTVYDYPNNLVDLFEESVTKFPDIRFLGTKNPEAGKYEWLT
                     RRQIAERVNNLRGALSKLGLSKGDRVGIIINNCVEWFVCEQAVHGLGAAFVPMYLQEL
                     PKTWKYIITDAAVKFLFVRDESVYEKVKHFKQEISTLQEILVVYGKGENSLSALEEMG
                     KKNPAPSVKPHWSDMAHIIFTSGTTGDPKGAMISHGNATWAARSSIDTFDLDETAQVI
                     SILPWAHVYGQNCDLHNYMAFGGGIAFVESLDKFMQNVQETKPTAINGVPRIVTKIYD
                     AIMMGVKADPMKKQFFDAALDVAAKNRDLPEKTKEFKDYDELVFSKVRAIFGGNLKFI
                     VSSSSLLKPEIAQFFHDIGQPTYDCYGMTETSGGVTMNCTKFGNKLGTVGKPIQHTNI
                     VIDKSRVGNDSPDGEICVYGPQIMMGYYNKPALNKEVMMPDQWNGFAGIRTGDRGWFD
                     EDGFLHITGRFKDEYKLENGKYVHPESIENEIKLLPQVMNAMVYGDGKQYNVAAIVPD
                     LSALKADPETKEWVKDTLEESLKNEDLKNYLSQKIMAHVRKSFGSYEVPQKFLYITED
                     FTVANSMLTQTMKLKRTNVLAKYKDQLLALY"
     gene            2708..4951
                     /locus_tag="PLG98_09870"
     CDS             2708..4951
                     /locus_tag="PLG98_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33609.1"
                     /translation="MIKNEKAITALLSTFPTAESFISFYQINDNSSICESFVGFAARN
                     GIITTPDIENLEQFIRAHTDRRENFRVTGDHNNFEELLKKKGETLRLNLSLRALCNRI
                     NALLTTHRIDLPQVTHSMLIRLKKEPLDTAYKLNVLRSIAFWLGYERPEISRTWNFET
                     LIKLFPEGAGSQKNGDYNEGVRIGFALTSRGEVIDHEIIGWLKKAVKSYIGEAIGHFL
                     YGKWGKVKAYDITTLYVDFPKEKEGGNLAHYMECLKSAVALAHQIAIRWPLSKYYSKN
                     RFLSIAVTAGEYGVLENHMLSLLNTGLPDDPMIRISDYARHGLLINDIHVILCPKPVE
                     ARLFNGESLPIWWITSIWTTHYFDFVPDLLHDETLQNSPASIEKLDRLLWPMGSEEAA
                     SGKTSDDNAIATFFKYPHHSLLGVEIAKTLYYRKRCSEAAEILRIVLSINPTDLVART
                     LRMMLLRNMALDTPSHRTAAAVFRQAIHEADNIREHCDFHTEDFYCEYAIVYLAQAMS
                     TVRYMRNHPEVRANMKEFKNLQRSVYKALDQAKQLFEKGMSVSSSGTRSSFLLKIAAV
                     LKTMLTADAELFVNPDKPILGGADIFQQESMDVQWQIGYRRSELPVQKQDELVVRITI
                     QKGDIYNAAISLGSYRPTTLFCNAVALWDFLPVHTVLTAKIVRERLQNAIDIARRAQD
                     ANVGIYAFTRTYSEMIPADEYIEQMQKALNIIDEEVGGNLSGRDNSEIIPGPADGRPV
                     KLFTLNF"
     gene            5213..6208
                     /locus_tag="PLG98_09875"
     CDS             5213..6208
                     /locus_tag="PLG98_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein RhuM/Fic/DOC family protein"
                     /protein_id="HOE33610.1"
                     /translation="MPHKGKSIEDISGGQIVLYQNNLEVRLMRETVWLNLNQMARLFE
                     RDKSVISRHLRKVFETSELDQELTVASFATVQKEGGRTIERNIEYFNLDAIISVGYRV
                     NSKRGTQFRIWATNVLRKHLVEGYSINQKRLEAQAGKIRELQEAVSLLSNVVALESIS
                     DETKGIIQIISEYSRGLGILDDYDHERLSMPGGTRRKTIIMTYEDAQEIITAMKKRFH
                     DSALVGREKDNSFKSSIGAIYQTFDGKDLYPTLEEKAAHLLYFVTKNHSFVDGNKRIA
                     AAMFISFLYKNRILLRKDGTKRIDDNALAALTLMVAASKPSEKDKLIKVILNLMH"
     gene            6362..6667
                     /locus_tag="PLG98_09880"
     CDS             6362..6667
                     /locus_tag="PLG98_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE33611.1"
                     /translation="MNTKEDILHCLLENKDILAALGVVSLGLFGSFVRGEQNPASDID
                     ILVEFSPDKHTFDNFMDVSFVLEDLLGRKVDVVTPEALSPYIGPYILKEVQRVAIAA"
     gene            6648..6998
                     /locus_tag="PLG98_09885"
     CDS             6648..6998
                     /locus_tag="PLG98_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOE33612.1"
                     /translation="MSPSPHEYLKHILDEIDYILSQTSGTDFQSFLKNETLQRAFVRS
                     LEIIGEATKKLPGSFRTDYPDIEWRKITGMRDRLIHDYFGVDYTIVWDVAVNKLPNLR
                     EKLKALMDQDLEVL"
     gene            complement(7077..7796)
                     /locus_tag="PLG98_09890"
     CDS             complement(7077..7796)
                     /locus_tag="PLG98_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOE33613.1"
                     /translation="MALEKLGEKTEREALVQHRQKEWLEVSRREMLIMSSGFVTALML
                     GNMSGAGLGHAAEIKFPEGKCTGKMEKKVLVTYASKYGSTGGVADVIAKELCARGVSA
                     DVALIKNARNIGSYQGVVIGSAIYMGQWMSEAVDFVKKNKEVLRKVPVAYFLVCMTLS
                     RPTEKNRAEVLAYMDPILKGVPEIKPVGLGTFAGAMHYNNLSWIYKKILKSKGTPEGD
                     YRDWNAIRSWAQEPVYAKFFR"
BASE COUNT         2017 a         1943 c         1987 g         2029 t
ORIGIN      
        1 tggcctggac gcccgtgggc ggcgacatgc ttttcatcga agccaccgcc atgaagggca
       61 aaaaaggcct gacgctgacc ggacaactgg gcgatgtcat gaaggagtcc gtcgcggcgg
      121 cgctgagctt tctgcgcgcc aatgcctgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcatca aggaaaaggt
      241 gctcgcggcc tatcgcgcgg gaatcaaaac cctcattttg cccgcgtgga accggaagga
      301 cctcgatgat attccggcca acgtgcagaa aaaagtgaaa ttccattttg tgagtgacat
      361 gatggaagtg ctgcggcttg cgctggagcc ggccaaagaa aaaacggtga aacaaaaggc
      421 agggaagaaa gcggcgaagt ccgggggcgc aaaacccgca gcaaaacggg ggaaaagcaa
      481 aacgaaaatt gcgcgcaaac cgattgcaaa aaagaaataa atttcccgag tttcgttaag
      541 aaaaaaagcc gcatcccgtg acggggatgc ggctttttta gcaaagtggc cggcggaaaa
      601 ttttaataaa gtgccagaag ctggtctttg tacttcgcca gcacgttggt ccttttgagc
      661 ttcatggtct gggtcagcat gctgttggcg accgtgaagt cttccgtaat gtagagaaac
      721 ttctgcggca cttcatagct gccgaaggat ttgcggacat gcgccataat tttttgagaa
      781 agataattct ttagatcctc gtttttcagg ctttcctcca gtgtgtcttt gacccactct
      841 ttggtttccg gatcggcttt caacgctgac aggtcgggaa caatcgccgc cacattgtat
      901 tgtttcccat caccgtaaac catggcgttc atcacctgcg gcagaagctt gatttcgttt
      961 tcgatgctct ccggatgcac atacttgccg ttttccagtt tgtattcgtc cttgaagcgg
     1021 ccggtgatat gcaggaaccc gtcttcgtcg aaccagccgc ggtcgcccgt gcgaatgccc
     1081 gcaaagccgt tccattgatc cggcatcatg acttctttgt tgagtgcggg tttgttgtag
     1141 taacccatca tgatctgtgg cccgtaaaca caaatctcgc cgtccgggct gtcgtttccc
     1201 acgcgggatt tgtcgatcac aatattcgtg tgctgaatcg gtttgcccac ggttcccaat
     1261 ttgttcccga atttggtgca gttcatggta acgcctccgg aggtttcggt catgccgtaa
     1321 caatcatacg tcggctggcc gatatcatgg aagaactgag cgatttccgg tttgagaaga
     1381 gagctggagc tcacgatgaa tttcaaattg ccgccgaaaa tggcgcggac cttggaaaag
     1441 accagttcgt cgtaatcttt aaactctttt gttttctccg gcaggtcccg gtttttggcc
     1501 gccacatcga gggccgcatc gaaaaactgt ttcttcatgg gatcggcctt cacgcccatc
     1561 atgatggcgt cgtaaatttt ggtgacgata cgcgggacgc cgttaatggc cgtgggtttg
     1621 gtctcctgaa cattctgcat gaatttatcc agtgattcga caaacgcaat cccgccgccg
     1681 aaggccatat aattatgcag gtcgcaattt tgtccgtaaa cgtgcgccca gggcaggatc
     1741 gagatcacct gcgccgtttc gtcgagatcg aatgtgtcga tgctggaccg ggcggcccag
     1801 gtggcattgc cgtgggagat catggcgcct ttgggatcac ctgtcgtgcc ggatgtgaaa
     1861 atgatgtgcg ccatgtccga ccagtgaggc ttgacggaag gagcagggtt tttctttccc
     1921 atttcctcca gggcggaaag actgttttcc cctttgccgt aaacaacaag gatttcctga
     1981 agcgtgctga tttcctgttt gaagtgtttg actttttcat aaacggattc gtcgcggaca
     2041 aacaggaatt taacggccgc gtcggtgatg atgtatttcc aggttttcgg cagttcctgc
     2101 agatacatgg ggacaaaagc cgcacccaat ccgtggacgg cctgctcgca gacaaaccat
     2161 tccacgcaat tgttgatgat aatgccgact ctgtcgcctt tggataaccc aagtttcgaa
     2221 agagcgccgc ggaggttgtt taccctctcg gcaatctgcc tgcgtgtcag ccattcatac
     2281 tttccggctt ccgggttctt ggtgcccaga aatcggatgt ccggaaactt ggttacactt
     2341 tcctcgaata aatcgacgag gttgtttggg tagtcataaa cagtattcgg gtccatttta
     2401 tctccttcca atctgttaag cattaaatct tcaggccttt tcagggagct gacatccatt
     2461 taaaaaagcc actcaagccg gttgaacatt ttagatcttc catgaatcag ttactgcaaa
     2521 atccttattg gaaataatct tataatgctg gcaggaagac agtcaatttg atatgttggt
     2581 ttacaaaaaa tattgaatta tctgtaaacc aataaaacaa aattaaaacg aatcgtgcaa
     2641 ataaacaggt tagggaaaaa tcatcaatat atcgtaagat aataatataa aaaggagaat
     2701 tgcaaaaatg ataaagaatg agaaggcaat aacagcactt ttgtcaacat ttcccacagc
     2761 agaaagtttc atatcctttt atcaaataaa cgataattca tcgatctgtg agtcatttgt
     2821 tggttttgcc gccagaaatg gcatcatcac aacgccggat attgaaaacc tcgaacagtt
     2881 tatccgcgct catacggatc gcagggaaaa cttcagagta acgggggatc ataataattt
     2941 tgaggaactg ctgaagaaga agggagagac gcttcgcctg aacctgtctt tgagggcttt
     3001 atgtaatcgg atcaacgcgc ttctgacaac ccaccggatt gatttgccgc aggttaccca
     3061 ttccatgctc atccgtttga aaaaagaacc actggacacg gcatacaagc tcaatgtttt
     3121 gagaagtatc gctttctggc tggggtatga gcggccggag atctcgcgga cgtggaattt
     3181 tgaaacttta atcaagttgt ttccggaggg tgccggttct cagaaaaacg gcgactacaa
     3241 cgaaggggtc cgaatcggtt ttgctttgac cagccgcggt gaagtcatcg atcatgaaat
     3301 tatcggatgg ctgaaaaaag ccgtcaaaag ttacatcggc gaggccatcg gtcactttct
     3361 ttacggcaaa tggggaaagg tcaaggccta cgatatcacg acgctgtacg tcgatttccc
     3421 gaaggaaaag gagggcggca acctggccca ttacatggaa tgcctgaaga gcgcggtggc
     3481 gctggcgcac cagatcgcca tccgctggcc gctttccaag tattacagca aaaacagatt
     3541 cttatcgatc gccgttacgg cgggagaata tggcgttctg gaaaatcaca tgttgtctct
     3601 tttgaacacc ggtctgccgg acgatcccat gatccgcata tcggattacg cgcgccacgg
     3661 tttgctgatt aatgatattc atgtcattct ttgtccgaaa cctgtagaag cccgattgtt
     3721 caacggggaa tccctgccga tctggtggat cacctctatc tggacgaccc attatttcga
     3781 ttttgtccca gatctgctgc atgatgaaac gttgcagaat tctcccgctt cgatcgaaaa
     3841 actggaccgg ctgctgtggc ccatgggaag cgaagaggcc gcgtccggca agacaagcga
     3901 cgataacgcg attgccacct tcttcaagta tccgcatcat tctttgctgg gtgtggaaat
     3961 tgccaagacc ctctattacc gcaaacgctg ttcggaggcg gcggaaattc tccggattgt
     4021 tttatccatc aatccgacgg atctggtggc ccggacattg cgtatgatgc tgctccgcaa
     4081 catggcgctg gatacgcctt ctcaccggac ggcggccgct gtttttcgcc aggccatcca
     4141 tgaagcggac aacatccggg agcattgcga tttccatacg gaagatttct actgcgaata
     4201 cgccatcgtt tatctggccc aggccatgtc caccgtgcga tacatgcgga atcatccgga
     4261 ggtccgggcg aatatgaaag agttcaaaaa tcttcaacga tctgtttaca aggcactgga
     4321 tcaggccaaa cagctttttg aaaaagggat gtctgtgtct tcttccggaa cccgctcatc
     4381 ttttttactg aaaattgccg ccgtgttgaa aacgatgctc acggccgacg cggaactctt
     4441 tgtcaatccg gacaaaccca tcctcggcgg cgccgatatc tttcagcagg aatcgatgga
     4501 cgtgcaatgg caaatcggct atcgccgcag tgaactaccc gtccagaagc aggatgaact
     4561 ggtcgtcagg atcaccatcc agaaaggcga tatctacaat gcggccattt ccctggggag
     4621 ctaccggccg accacgctgt tttgcaatgc ggtggccctg tgggattttc tgccggtcca
     4681 tacggtgctc acggcaaaaa tcgttcggga gagattacaa aatgccatcg acatcgcccg
     4741 tcgggcgcag gatgccaatg tcggcatcta tgcctttacc cgaacctaca gtgaaatgat
     4801 tccggcggat gaatatattg agcagatgca aaaagcgctg aacatcatcg acgaagaagt
     4861 cggcggcaat ctgagcggtc gcgacaattc ggaaatcatt ccgggcccgg cggacggcag
     4921 gccggtgaaa ctctttacgc tgaattttta accggcgtcc tttagatttg taagagatga
     4981 gggggggaga ctttgcacaa tcaatggttc cgttcttccg gcgaagagac tatgctgcaa
     5041 tgaatcatcg tcatggcgag tcccggcgcg tcgggacgtg gcaatctatt ttctgcgctt
     5101 tcacttgtca ccatccacca taaactattt ttattcttgc attgttttca ataagaggta
     5161 agatttttct acagttcatt tatcataaat cattttacgt gaggcgcatc ccatgccgca
     5221 taaagggaag agcatagaag atatttccgg cggtcagatt gttctttatc aaaacaatct
     5281 ggaagtcaga ttaatgcggg agacggtttg gctgaatctg aaccaaatgg caagactttt
     5341 tgaaagggac aaatctgtta tttcccggca tttgcgaaaa gtttttgaaa caagtgagct
     5401 ggatcaagaa ttaactgttg catcttttgc aacagttcaa aaagaaggcg gccgaaccat
     5461 tgaaagaaac attgaatatt tcaatctgga tgccatcatc tccgttggtt accgggtgaa
     5521 ctccaaacga ggcacccaat tccgcatctg ggccacaaac gttttgcgca agcacctggt
     5581 ggaaggttat tccatcaatc aaaaaaggct tgaggcgcag gccggcaaaa tcagggaatt
     5641 gcaggaggcg gtgagccttc tttccaatgt tgttgccctg gaaagcatct ccgacgaaac
     5701 aaaaggcatc attcagatca tttccgagta ttcgcggggg ttgggtattc ttgatgatta
     5761 cgatcatgaa cgtttgtcga tgccgggtgg taccaggcgg aaaacgatca taatgaccta
     5821 tgaagatgcc caggagatca tcaccgctat gaagaagagg ttccacgatt cggcgctggt
     5881 tggccgggaa aaagacaaca gtttcaaaag ctccatcggc gcaatctatc aaaccttcga
     5941 cggcaaggat ctttatccga ccctggaaga aaaggcggca cacctgctgt attttgtaac
     6001 caagaatcac agtttcgttg acggcaacaa acgcatcgcg gcggccatgt tcatcagctt
     6061 cctgtataag aacagaattc ttctgcgaaa agacggcacc aagcgaatcg acgacaacgc
     6121 gcttgccgcg ctcacgctga tggtcgccgc cagcaagccg tccgaaaaag ataaactgat
     6181 caaagtgatt ttgaacttga tgcattaaat tgaggcacgg cggcctgttg gagcaagaaa
     6241 ttatattgtg gaacgaatag aagcttggtc ttttacgaag ttattttgtg atattctcat
     6301 tgacttcgtc aattattcct tgtatttttc aggccataga taagtaagta atgagacgca
     6361 catgaatacc aaagaagata ttttacattg ccttcttgaa aataaagaca tcctcgccgc
     6421 tctgggcgtt gtcagcctgg gcctgttcgg ctcgtttgtg cgcggcgagc aaaacccagc
     6481 aagcgacatc gacattcttg tagaattttc tcccgacaaa cacacattcg acaatttcat
     6541 ggacgtatct tttgtgctgg aagatctctt ggggcgcaag gttgacgttg tcacgccgga
     6601 agcattgagt ccttacatcg ggccctatat tctgaaagag gtgcaacgtg tcgccatcgc
     6661 cgcatgaata tctgaaacat attctggacg agatcgatta catcctttca caaacgtcag
     6721 gaacggattt tcaatctttt cttaagaacg aaacgctgca aagagccttt gtccgcagcc
     6781 tcgaaatcat cggtgaggcc acaaagaaac ttccgggctc cttccggacg gattaccctg
     6841 atattgaatg gcgcaaaatt accgggatgc gcgaccgact gattcacgac tacttcggcg
     6901 tggactacac tatcgtctgg gatgttgccg tcaacaagct accaaacctc cgtgaaaagt
     6961 tgaaggcgct aatggatcaa gatttagaag tgttgtaaaa agtaccgtct ttgcaaaaag
     7021 agtgtttcac tgactgatcc cttggtattg tcattcttta ccggcttcgg gattttttac
     7081 cggaagaatt tggcatacac gggttcttgt gcccaggaac ggatggcatt ccagtcgcgg
     7141 taatctccct cgggagttcc cttggacttc agaatctttt tgtatatcca ggacaggttg
     7201 ttgtaatgca tcgcgcctgc aaatgttcca agacctacag gctttatttc cggtactccc
     7261 ttcaggatgg ggtccatata agccagcacc tcggcccgat ttttctcggt cggtctggag
     7321 agcgtcatgc acacgaggaa ataggctaca ggcaccttcc gcaaaacttc tttgttcttt
     7381 ttgacaaaat ccaccgcttc cgacatccac tggcccatgt agatggcgct cccgatgacc
     7441 acgccttgat aggaaccgat gttacgggca ttcttgatca aagcaacatc ggcgcttacg
     7501 cctctggcgc acagctcttt tgcgatgaca tcggcaactc ccccggttga accgtacttg
     7561 ctggcgtacg tgaccaatac tttcttttcc attttgcctg tgcatttccc ttccggaaat
     7621 tttatttccg ctgcgtggcc gagcccggcc cccgacatat ttccaagcat cagggccgta
     7681 acaaagccgc tggacatgat cagcatttcc cggcgactga cttccaacca ctccttttgc
     7741 ctgtgttgca caagagcctc cctttctgtt ttttcaccta atttttccag tgccatatac
     7801 tcctgatgcg ttcaaaatgg aagcccatac ccggaggatg atacagagat gccggaggtc
     7861 gggcgcattc aaattgttcc aaagtgaaac agcgttccag tgtttttacc tgcaaaaaca
     7921 tttcatcctg cgccgtattt ttttctgaga gtcctgtacc ggagactgtt tcgcggattc
     7981 agcaacggcg gcatggcgct gggggcggcc tcgggaacct gaagctgccg gagcgtatga
     8041 agggaagcct
//
LOCUS       DAOSME010000080         7940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_10201_length_7940_cov_15.740013, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000080 DAOSME010000000
VERSION     DAOSME010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10201_length_7940_cov_15.740013"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLG98_09895"
     CDS             complement(<1..847)
                     /locus_tag="PLG98_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOE33614.1"
                     /translation="MTDSPIKSALKDFFFFWKPSLARRLTLSFTIFGLVIGYAVLIYL
                     AVSSTNTLIRQASVSVEEYLTYMSREDLRENQGELLKFIDQKMTNIVHMAKAMQVVLL
                     PVQFELYFKKDGQWQHTFTDEQGIVTSEIISNPGLAGLLEVAKSRKMTTSSKFFYGRH
                     DKVNVKINISPDKNNYTQLLAFDIYRVGILKILRENIYKAFFFFVLLLVICHMMGHIF
                     SYLLARPIEQLSREAEAIASGQYERRFTSNRQDEIGKLADALNIMASRILDSTKEREN
                     IFIGIL"
     gene            1130..2038
                     /locus_tag="PLG98_09900"
     CDS             1130..2038
                     /locus_tag="PLG98_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOE33615.1"
                     /translation="MEYKNVDDVQSSLTDAGYIPSREIATVVFLAAATQKPVLVEGPA
                     GVGKTELAKAIARSLERDLIRLQCYEGLDEAKALYEWEYAKQLLYTQMVKDRLNDIIS
                     SAATLTEAVDQIAAQEDAFFSDRFIQHRPLLQAISSEKPVVLLVDEVDKADPEFEAFL
                     LEILSDFQVSIPELGTRKAIQIPFVLLTSNNYRDMSDALKRRCVHLYIDYPARDTEIS
                     IVRLKIPGIEEKLCDALVDAIRKIRKLDLKKNPSISETIDWAQSLIALQINELTPEVV
                     SETLNIICKYQADMEKVRKNINRVLQ"
     gene            2278..3609
                     /locus_tag="PLG98_09905"
     CDS             2278..3609
                     /locus_tag="PLG98_09905"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE33616.1"
                     /translation="MRISTSEVLDSLHQMKLINPVDEQQFRSMLRSNFAKSMREVQHF
                     DRLYHLFFHEMRIDTSDIRQAADYSTEINMTGDALKKIPHDNPIYDAVVDFLQGNPMA
                     LLREMRRIRTEEDVTMLRFNLGPLGNRLNVMLQINKAASDARTLVEDHYMKTDSSAGR
                     KLAAYFEERVSAARSMLVYEPQQGESGTRKTKTEEQKLRGLGEKSFSSFTRQEVEEMR
                     EAIDKMVRKLKNIVSRRYAVRNRGSIDIKKTLRASAKYNGVPVDVKYRSRAKRKSRIV
                     TLCDVSGSVWAAARFMLNLLYALQDCFDKVNSFVFIDHLSDVTSIFEKNEINEAIRLV
                     LEKPDINFNASTDYGETLRNFKKDYMDLLTKKTTLIIVGDGRTNYMNPEDAILGQMRE
                     RCRRIIWLNPEQENLWSSGDSEIGSYMPHCHEVRQCRNVNQLFTFIEELVL"
     gene            3611..4735
                     /locus_tag="PLG98_09910"
     CDS             3611..4735
                     /locus_tag="PLG98_09910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE33617.1"
                     /translation="MQTTTKDQSYSWISRFAIEHVASRNDLQTMQDFPLDIWQKMGEA
                     GFFKLGISPQFGGSGGGLTQLAGAGEVFVRSGFNMGLALSWLYQQIIAIHLMSGFGTE
                     EQKKRYLPLMANGSLTVSFAVSEPKHGARPKLLTSTADLQDTSYILNGEKTYLTNAPI
                     AGLFIVIAITGRDKDQKRFSAFLVPADHEGLMIKPRLKFDFLKPAPHGGIKLNNCRIP
                     ASAILGENDSAYANIVIPFSDAENTVLMGAVTGGMGAELMDLIMESDKRNIHKDKALQ
                     SEVGALDSLLETMRAIVRETAKKVDERDNAAVPLIVTFKELAAGFQARIAAAIAKWDI
                     KTPDRFKVLQTDINVLGALQEKTLQDKQAKLGDELLHGAR"
     gene            4778..5257
                     /locus_tag="PLG98_09915"
     CDS             4778..5257
                     /locus_tag="PLG98_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOE33618.1"
                     /translation="MMTLLWILAVLFILIGVAGTLIPALPGVPLILIGVLIGAWIDDF
                     QRISAATVVVMVILTVLGVVIDYVAAALSAGRAGASRQGLIGAVLGTLAGLFTGFWGL
                     LFMPLAGAAIGEFIARRDMLHAGKVGAATWFGLVISVVVKLAIAFTMVGVFIAALLI"
     gene            5307..6068
                     /locus_tag="PLG98_09920"
     CDS             5307..6068
                     /locus_tag="PLG98_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOE33619.1"
                     /translation="MNEKLLKTYRLFLKDTIRKATDFSQTDQSRGIPPPPIEKPCSGT
                     ARRVDLVRYDQFQNIGTLDLKTAIRNRESRRTYGTQPLTLGELSFLLWATQGIKQILD
                     AGHALRTVPSAGCRHALETYLCILNVQGIAEGIYRYLPLEHQLLHEQTVENLKIKIRQ
                     AVFGQRYPADAAATFIWTAVPRRMEWRYGIAAHKVIALDAGHVCQNLYLACEAIGAGT
                     CALAAYDQEEMDRLLSIDGEEEFTIYLASVGKKPF"
     gene            complement(6094..>7940)
                     /locus_tag="PLG98_09925"
     CDS             complement(6094..>7940)
                     /locus_tag="PLG98_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOE33620.1"
                     /translation="AVFWVGSRFFIGAYKAALQKTSDMNTLVAVGAFSAYAYSAAATF
                     SPQFFTTAGITPHVYYDGAAMIVTLILLGRLLEAGAKSKTSMAIKNLIQLKPKTAHLI
                     REGQELDVAVDEVRIGDVVFVRPGERIPVDGTILSGESTIDESMLTGESIPVSKVAGQ
                     KVFAATMNTTGGFTFQATGVGAETALAQIIRLVQEAQGSKAPIQRLADKVVSVFVPAV
                     FLIALMTFAVWYFLSAEGNFSRALISFVSVLVIACPCALGLATPTAIMVGTGLGAQSG
                     ILIKGGEALEKIHKLSVIVFDKTGTLTKGEPDVTDILTADGYEEKQILSFAAALEKSS
                     EHPLARAIVRKAEQKSVRIETVEKFTALSGLGAKALMDGQRVLIGNRFLMTDEGIAID
                     DWNLSTERLTGEGKTVVYLALGKSVVGLIALADTPKASAARTVQRLKSRGMKVAMITG
                     DNEGTARAIARQLGIEKVMANVLPGKKAEEIKNLQDAGEIVAMVGDGINDAPALAVAD
                     VGIAIGAGTDVAIESGDITLIRDDLLGVPDSISLSQATMRTIRQNLFWAFIYNVVGIP
                     VAAGVLYPFFGILLSPEFAAAAMALSSFSVVSNSLRLRRVWEKKIARG"
BASE COUNT         1951 a         2090 c         2026 g         1873 t
ORIGIN      
        1 tcaggatgcc gataaatata ttttctcttt ccttggtcga atccagaatc cgggaagcca
       61 tgatattgag cgcatccgcc agtttgccga tttcatcctg acgattgctc gtaaaacggc
      121 gttcatattg tcccgatgcg atggcctccg cctcccggga tagttgttct atgggccgcg
      181 ccaggaggta actgaagata tgccccatca tatgacaaat cacgagcagc aaaacgaaaa
      241 agaaaaacgc cttgtagata ttttcccgaa gaatcttgag gatgccgaca cggtaaatgt
      301 caaaggcgag aagttgcgta taattatttt tgtccgggct gatattaatc ttcacattga
      361 ctttatcatg tcgtccgtaa aaaaattttg acgatgtcgt catcttccga ctttttgcca
      421 cttccagcaa tccggcaagt ccgggattgc tgattatttc ggatgtgacg atcccctgtt
      481 catcggtaaa cgtatgctgc cactgcccgt ctttcttaaa ataaagttcg aattgaacgg
      541 gcagcagcac cacctgcatg gcttttgcca tatgaacaat attggtcatc ttctgatcaa
      601 tgaatttaag aagttcgccc tgattttcac gcagatcttc cctggacatg tacgtcaggt
      661 attcttctac cgaaaccgat gcctgtctga ttaacgtgtt ggttgaagaa accgcgagat
      721 aaatgagaac ggcgtaaccg atcaccaggc cgaaaattgt aaaagaaagt gtcagccttc
      781 gggccaggga aggtttccag aaaaagaaaa aatctttcag ggctgatttt atggggctgt
      841 ctgtcatttc agagacgtta ttttcctatt tcaaatcaat tttggtttat catagcatga
      901 aaggatggct gagaaaaatt ctttttcatg atcgttgttg acggtaggaa gcgctggtcg
      961 aatgatccag tagatttgat tatccccgac gcgaaacaaa gcggattcag gaactctctg
     1021 atgctgagaa tatatactcc ttgagtcggt gttggatctc gattcctgat tgacataccc
     1081 tgataaatca cctatgcttt gcgcaattcg aaaaggaatt cattctctta tggaatacaa
     1141 aaacgtagac gatgttcaat ccagtttgac agatgccgga tatattccgt cgcgggaaat
     1201 tgcgacagtc gtttttctgg cggcagcgac ccagaaaccg gtgctggtcg agggaccggc
     1261 cggcgtcggc aaaacggaac tggccaaagc catcgcccgc tccctggagc gcgatctgat
     1321 ccggcttcaa tgttatgaag gtctggacga agccaaggcg ctttacgaat gggaatacgc
     1381 caaacagctc ctttacaccc agatggtcaa ggacagactc aacgacatca tctcttccgc
     1441 cgccactctt acagaggcgg tggatcaaat tgccgcgcag gaagacgctt tcttctccga
     1501 ccgttttatt cagcatcggc cgctgctgca ggcgatcagt tcggaaaaac ctgtcgtgct
     1561 tctcgtggat gaagtggaca aggccgatcc ggaatttgag gcatttcttc tggaaatatt
     1621 aagtgatttt caggtgtcca ttcccgaact gggcacccgc aaggccattc agattccctt
     1681 tgtgctgctg acatccaata actaccgcga tatgagcgac gcgctgaagc gacgctgtgt
     1741 ccatctctac attgattatc cggcgcggga tacggaaatt tccattgtgc ggttgaagat
     1801 tccgggcatc gaagaaaaac tctgcgacgc tcttgtggac gcaatccgca aaattcgcaa
     1861 gctggatttg aaaaaaaatc cgagcatttc ggaaacgatt gactgggcgc agtcgctgat
     1921 tgctctgcaa atcaacgaac tgacacctga agttgtttcg gaaacgctga atatcatctg
     1981 caaatatcag gcggatatgg aaaaagtccg taaaaacatc aaccgggtcc tgcagtagtt
     2041 taaaatgtca ttccgaattc cgacgtctcg ggatgagaaa tctttacgat gctgatatct
     2101 gaagacttct ccccaacgta gcaaaacgat agccttcagt cgaattgaca taacgcttat
     2161 cgtggagtag gatcccgtat atgcggacgg ctaaatgaca gaacaatcga ttatttttat
     2221 ctgtaattta tggtcgggct tattcaacaa tttgtttcct gctgcagggc gtcgggcatg
     2281 aggatttcga catccgaagt cctggacagc ctccatcaga tgaaactgat caatccggtg
     2341 gacgagcagc aattccggtc gatgcttcgt tccaattttg ccaaaagtat gcgcgaggtt
     2401 cagcactttg accggctgta tcatcttttc ttccatgaaa tgcgcataga cacatccgat
     2461 atccggcagg cggccgatta ttccaccgaa atcaacatga cgggagatgc tcttaaaaaa
     2521 atcccccatg acaatccgat atacgatgcc gtagtggatt ttttgcaggg gaaccccatg
     2581 gcgcttctac gggaaatgcg ccggattcga acggaagaag acgtgaccat gctgcgtttc
     2641 aatctgggac cgctgggcaa ccgccttaat gtgatgctgc agattaacaa ggccgcgtct
     2701 gacgcccgga ccctggtgga agatcattac atgaaaacgg attcttcagc cgggcgcaag
     2761 ctcgccgcct atttcgaaga acgcgtaagt gccgcccgtt ccatgctcgt ttacgaaccg
     2821 cagcagggag aatccggcac ccggaaaaca aaaacggaag agcaaaaact caggggactg
     2881 ggcgaaaaat cgttttcttc tttcacccgg caagaagttg aagaaatgcg cgaagccatc
     2941 gacaagatgg tgcgcaaact gaaaaatatt gtcagccgcc gatacgccgt ccgcaacagg
     3001 ggaagcatcg atatcaaaaa aacactgcgc gcatcggcca aatacaacgg ggttccggtg
     3061 gacgtcaaat accgcagccg ggcgaaacgc aaaagccgca ttgtcacgct ctgcgatgtg
     3121 tccggttccg tctgggcggc ggcgcgtttc atgctgaatc tgctttacgc cctgcaggac
     3181 tgttttgaca aggtcaacag ttttgtgttt attgatcatc tttcggacgt cacgtccatc
     3241 tttgaaaaaa atgaaatcaa cgaagcgatt cggctggtgt tggaaaaacc ggatatcaat
     3301 ttcaacgcat caaccgatta cggggaaacg ctgcgcaatt ttaaaaagga ttacatggac
     3361 cttctgacca agaaaacaac gctgattatt gtcggcgacg gccgcaccaa ctacatgaac
     3421 cccgaagatg ctattttagg ccagatgcgt gaacgctgcc ggcggattat ctggctcaat
     3481 cccgagcagg aaaatctctg gagttccggc gacagcgaga tcgggtctta catgccgcat
     3541 tgtcatgagg tgcgtcagtg ccgcaatgtc aatcaattat ttacttttat agaggaactg
     3601 gttttataaa atgcagacga caacaaagga tcaatcgtac agctggattt cacgttttgc
     3661 cattgaacac gtcgcttcac gcaacgattt gcagacgatg caggattttc cgctggatat
     3721 ctggcaaaaa atgggagaag cgggattttt taaattgggc atctctccgc aattcggcgg
     3781 cagcggagga ggattaacgc aactggccgg cgccggagag gtattcgtcc gcagcggttt
     3841 caacatggga ctggctctgt cctggcttta tcagcagatc atcgccattc atctgatgtc
     3901 cggtttcggc acggaagaac aaaaaaaacg ttatctgccc ctgatggcca atggaagtct
     3961 gacggtttct tttgccgttt ccgaaccgaa acacggggcg cggccgaagc tcttgacgtc
     4021 cacagccgat ttgcaggata catcctacat cctcaatgga gagaaaacat atctgaccaa
     4081 tgcgccgatt gccggccttt tcatcgtgat cgccatcacc ggccgggata aagatcagaa
     4141 gaggttcagc gcctttctgg tgcccgccga tcatgaaggc ctaatgatca agcccaggct
     4201 taaattcgat ttcctgaaac ctgcgccgca cggcggcatc aaattaaaca attgccgcat
     4261 ccccgcttcc gccatcttgg gagaaaacga ttccgcctat gcaaatatcg tcatcccgtt
     4321 tagcgatgcg gaaaacacgg tgctgatggg cgcggtcacc ggaggcatgg gcgcggagct
     4381 catggatctg atcatggaaa gcgacaaacg caacattcat aaagataagg cgctgcaaag
     4441 cgaagtcggc gcgctggatt ctctgctgga aacgatgcgc gccattgtcc gtgaaaccgc
     4501 caagaaggtt gatgaacgcg acaatgccgc cgttccgctg atcgtcacct tcaaagaact
     4561 ggcagccgga tttcaggccc gaatcgccgc cgccatcgcc aaatgggata ttaaaactcc
     4621 ggaccgcttc aaggttttgc agaccgatat taacgtcctg ggagcattgc aggaaaaaac
     4681 ccttcaggac aagcaggcga aactgggcga tgaactgctt cacggcgcgc gataaagatt
     4741 gttccatcga ttcgaagagg gttgagtctt tcatggtatg atgacgttgt tatggatcct
     4801 tgctgttttg ttcatcctga tcggcgtcgc cggaacgctg ataccggctc ttccgggcgt
     4861 tccgctgatc ctgatcggcg tgctgatcgg cgcctggatc gacgattttc agcgcatcag
     4921 cgcagcgaca gttgtggtga tggtgatcct gacggtcctc ggtgtggtga ttgattacgt
     4981 tgccgcagcg ttatccgccg ggcgcgcggg ggcaagccgg cagggactga tcggtgcagt
     5041 cctgggaacc ctcgcgggcc tgtttaccgg attctggggg cttttattca tgccgctggc
     5101 cggggccgcc atcggcgaat tcatcgcccg tcgggatatg ttgcatgcgg gaaaagtcgg
     5161 tgccgccacc tggttcggat tggtcatttc tgttgtggtg aaactggcaa ttgccttcac
     5221 gatggtcggc gtgtttatcg ctgcgctgct gatctgagaa gagattgacc cggccgcaaa
     5281 tccggctccg gatacaggat gcagccatga atgaaaaact tctgaaaacc tacaggcttt
     5341 ttctcaaaga taccatccgt aaagccactg atttttctca gacggaccag agcagaggga
     5401 tccctcctcc gcccatcgaa aaaccctgct ccggcaccgc ccggcgcgtt gatcttgtgc
     5461 gctatgacca gtttcaaaac atcggcacac ttgatcttaa aacagccatc cgcaaccgcg
     5521 aaagccggcg aacctacggc acgcaacccc ttaccctcgg agaattgtct tttctcctgt
     5581 gggcaacgca ggggatcaaa caaatccttg acgccggtca cgccttgagg acggttcctt
     5641 cggccggttg ccgtcacgct ctggaaacgt atttgtgtat tctgaacgtt cagggaatcg
     5701 ctgagggcat ttaccgttat ctcccgctgg agcaccagct gttgcatgaa cagaccgtgg
     5761 aaaatctaaa aataaaaatc cgtcaggcgg ttttcggcca gcgttatccc gcagacgcgg
     5821 cggctacatt catctggacg gccgttcccc gcagaatgga gtggcgttac ggtattgccg
     5881 cccacaaagt catcgcgctt gatgccggtc atgtctgcca gaatctttac cttgcctgcg
     5941 aagctatcgg cgccggaacc tgcgctctgg ccgcttatga tcaggaagaa atggaccggc
     6001 tgctcagcat cgacggcgag gaagagttca cgatctatct ggcctctgtt ggaaaaaaac
     6061 cgttttgata ccgggaggcg gtcaacccgt ccgttacccg cgcgcgattt tcttttccca
     6121 cacgcggcgc agccgcaggg aattgctcac caccgaaaac gaacttaggg ccatggcggc
     6181 ggcggcaaac tcgggactga gcagtatgcc aaagaaagga tacaagactc ccgcagccac
     6241 gggaatgccg acaacattat aaataaacgc ccagaagaga ttctgcctga tcgtgcgcat
     6301 ggtggcctgt gataggctga tcgagtccgg tacgcccagt aaatcatcgc ggatcaacgt
     6361 gatgtcgccg gattcaatcg ccacgtccgt tcccgccccg atggcaatac cgacatcggc
     6421 gaccgccagc gcaggcgcat cgttgatgcc gtcgccgacc atggcgacaa tctcgccggc
     6481 atcctgcaga ttttttattt cctccgcctt tttccccggc aggacattgg ccataacttt
     6541 ttcaatgccc aattgtctgg cgatggcgcg ggccgtgcct tcgttgtctc ccgtaatcat
     6601 cgcgaccttc attcccctgc tcttgagacg ctggaccgtt cgcgcagccg aagctttcgg
     6661 tgtatcggcc agggcaatca accccacaac cgattttccc aaagccagat aaacaaccgt
     6721 ctttccttct ccggtcagcc tttccgtaga aaggttccag tcatcaatgg caatgccttc
     6781 gtctgtcatt aagaaacggt tgccgataag aacacgttgg ccgtccatca gggctttggc
     6841 tcccagaccg gacagcgccg tgaatttttc aaccgtttcg attctcacgc ttttctgttc
     6901 ggctttccgc acaatggcgc gggccagcgg gtgttcggaa cttttttcca gggctgcggc
     6961 gaacgacaga atctgctttt cctcataacc gtccgcggtc aaaatatcgg taacatccgg
     7021 ttctcccttc gtcagggtgc ccgttttatc aaaaacgatc accgaaagtt tatggatttt
     7081 ttccagcgct tcgccgcctt tgattaaaat gccgctttgc gctcccaggc ctgtgcccac
     7141 catgatggct gtcggtgtcg ccagtcccag agcgcaggga caggcaatca ccagcacgga
     7201 aacgaaactg atcaaggcac ggctgaaatt cccttccgcg gaaaggaaat accagacggc
     7261 aaaggtcatc aaggcaatca gaaacacggc cggaacaaaa acggagacaa ccttatccgc
     7321 cagacgctga atcggagcct ttgatccctg agcttcttgc accagacgga tgatttgcgc
     7381 cagcgcagtt tctgcgccca cgccggtcgc ctgaaacgta aacccgcctg ttgtgttcat
     7441 cgtggcggca aaaacctttt gtcccgccac tttactaacc gggatacttt caccggtcag
     7501 catggattcg tcgatggtcg attcccccga cagtatcgtg ccgtccacgg gaatccgttc
     7561 cccgggcctg acaaaaacga catcgccaat tcgtacctca tccacggcga cgtcgagttc
     7621 ctgaccctcc cggatgagat gcgctgtttt cggctttaat tgtatcaggt tttttatcgc
     7681 catggacgtc ttgctcttcg ccccggcttc cagaagtctg cccagcagaa tgagcgtgac
     7741 aatcatggcc gcaccgtcat aatacacatg cggtgtaatg cccgccgtag taaagaattg
     7801 aggggaaaaa gtcgcggcgg ccgaataggc gtaagctgaa aaagcgccga cggcaaccag
     7861 tgtgttcatg tcggatgttt tctgaagggc ggctttgtag gcgccgataa aaaagcggct
     7921 gcccacccag aagaccgccg
//
LOCUS       DAOSME010000081         7861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_10337_length_7861_cov_24.156290, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000081 DAOSME010000000
VERSION     DAOSME010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10337_length_7861_cov_24.156290"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLG98_09930"
     CDS             <1..480
                     /locus_tag="PLG98_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33621.1"
                     /translation="IAKSKPVLGICLGAQLIASALGAGVYPNKVKEIGWFPIHGIESN
                     GQSVFAFPESISVFHWHGETFDLPSGATLLAKSDACEHQAFQVGKSVIGLQFHLETMP
                     AAAQALVTNCRDELVPAPFIQTEEEILSTTPDQYTAINDLMADILSFLLTQKMTAGL"
     gene            696..2258
                     /locus_tag="PLG98_09935"
     CDS             696..2258
                     /locus_tag="PLG98_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE33622.1"
                     /translation="MTKTSKKSLEERESQLSIHTYEELAALHAIAQILAQPGDLHHQL
                     ALILKEMSSRLGMQRGMISLVDRDNKESWLDVAHDVNIDGLEITYKHGEGITGKVAET
                     GRPMAVANLGQETHFLDRTGARRHLNRAELSFLCVPIIYDFMVEGVLSADKVAVKVEN
                     LDKELALMASVAELMAKAVHIRALEEENKRLRKIIGSTRAPLMEIIGHSKAMQEVFGL
                     VAQVADSNTTVLITGETGTGKELIARAIHKNSVRKNGPMVQVNCAAIPDTLIESELFG
                     HEKGAFTGALTQRRGRFEEANGGTIFLDEVGELSAAAQVKLLRVLQEKRFQPLGSSRV
                     VNVNVRIIAATNRNLEQDILSDRFRADLFYRLNVFPIYLPPLRERGSDVIMLADHFLL
                     KYSKEMGKQVKRISPAAIEVFLSHKWPGNVRELENCIERGVLMTKTDVLDCEHLPPSL
                     QLKDGMHDRKGRGKLSSVVEAQERLLIIDALKETSGNQTKSAKLLGTTKRIIQYKISK
                     MGIDPKNYRKKS"
     gene            2505..3635
                     /locus_tag="PLG98_09940"
     CDS             2505..3635
                     /locus_tag="PLG98_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOE33623.1"
                     /translation="MCRLFGMTSNDPVSPLAAIRAIDVMREGHDGSGVGLFLTDLGSD
                     FQEFKNEPILSGIFSNEGLKTLDRFMIDHDFMVKYKLSIRPAKNPPAGTPKRDNYVIR
                     IYEYPEEWDGLCRDEIEFRLMMVQLELRRMGEQDESMLVFSFWPDVIIIKEVGDPLAV
                     AEYLGLDRKELMARVILSQGRQNTNYAINIYACHPFFIQGMATVTNGENTAFVPIREF
                     LMSRKFPGYTGYHSDSETFTHILHYMYHQLGMGMEMYKHIITPLKDGELNRHPDGVWL
                     RKLKQSCRPLIIDGPNCVIGCLPDKSMFMVQDSKKLRPGVVGGRPGVFAFSSEMCGLD
                     AALPDRYVNLDFQPMKYETVIVRSERQEIEKWNQLEPLTHPH"
     gene            3599..5233
                     /locus_tag="PLG98_09945"
     CDS             3599..5233
                     /locus_tag="PLG98_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOE33624.1"
                     /translation="MESAGTVNPSTLSVKDLPWQIMWNKEKCTLCGRCTAVCPVRAIE
                     PGVHRKRALQVPVGLEEKPAQLLSVYFGIDQRTDAAHACVGCGMCTLVCPNGAIMPVR
                     AEETDKLRFHVNQGGEPRRRGGRRNVPDSVLDKIKFIRISMLTDPALDAGRHEFELRT
                     LLGRVLPPEELLKVHRENGWVPPVREIYPLVIGGMSFGALSPNMWEGLQMGVAYLNEE
                     LGMPVRICTGEGGCPPRLLRSRFLKYVILQIASGYFGWDEIIHGLPQMKEDPCAIEIK
                     YGQGAKPGDGGLLMWYKVNKLIAAIRGVPQGVSLPSPPTHQTKYSIEEAVAKMIQSMS
                     MAWGFRVPVYPKISATSTTLAVLNNLTRNPYAAALAIDGEDGGTGAAYNVSMNHMGHP
                     IASNLRDAYLNLVKIGKQNEVPLFAGGGIGKNGNLAANAAALIMLGASGVQTGKYIMQ
                     AAAGCLGSETDRCNICNIGACPKGITSQDSRLYRRLDPEKVAQRLVDVFLSFDTELKK
                     IVAPLGRSTSLPIGMSDALGIADAGAAERLKIKYVL"
     gene            5255..7591
                     /locus_tag="PLG98_09950"
     CDS             5255..7591
                     /locus_tag="PLG98_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE33625.1"
                     /translation="MTKKESLKVTGQENGIRVESRILEERIQQAVAEGYRHIEITAYG
                     QHGIGGRLWRAGETPIKVDVTGTSGQRLGSMGFANTLIEVFGPVSDDVGWLNAGAKII
                     VHGNATNGVANAMAQGKVFIDGDIGARGMTMTKHNPRFDPPELWVFGKAGDSFAEFMA
                     GGIAVICGVGAENEDDVLGYRPCVGMVGGKIFFRGRQSSYSTQDARLITDLPDEEWRW
                     LTKNLKAFLQDVGKQYLCEELTARRSDWKLLSALKPHEKKGRALSPMTVYRKEAWEKE
                     LGTGGLIGDLTGADQSPIGLITTGDLRRFVPVWANEKYLPPCQASCPTGIPVQKRWEL
                     IRKGKMKDAVDLALRYTPFPATVCGYLCPNLCMQNCSRKIVSLPSVDVTVLGKASLEA
                     KEPKPLPATGKKIAIIGGGAAGLSVAWQLWMNGHDAVIFESRKHLGGKITQTIPQSRI
                     PDNVVEHELKRLAKNLRHIHLNASLTKEKFRKLKNEFDFIVIAAGAAKPRKLNVPGME
                     KALTALEFLKQSKGNGINVGKKVVIVGAGNVGCDAATEAFRLGAKTVTLIDVQQPASF
                     GTERKHAEAAGAIFLWPRFTKAITARGVELTDGEILPADTVIVAVGDVPDISFLPEEI
                     AVKNGFIAVDEQFRTSDDQIFAVGDVTKPGLLTEAIGAGRIAAGTIDALLRGAGETYD
                     KLPPIDVQRVKTEYFDSRIKAFSDPVSCAVNCASCGACRDCGMCETICPQNAISRKAL
                     NGENYEYVVDAELCMGCGFCAGVCPTGVWEIVDNKPLE"
     gene            7622..>7861
                     /locus_tag="PLG98_09955"
     CDS             7622..>7861
                     /locus_tag="PLG98_09955"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOE33626.1"
                     /translation="MKKTKKTTAAEREYVIEKEKFVPVSKYFGEDTFNQKTMKERLPK
                     ETFKKLMDAINEDKRLDLKTADVVAEAMKKWALEKG"
BASE COUNT         1976 a         1900 c         2191 g         1794 t
ORIGIN      
        1 atcgctaaaa gtaaaccggt gctgggaatc tgcctgggcg cgcagcttat tgccagcgcg
       61 ttgggcgccg gagtgtatcc caataaagta aaagagatcg gctggtttcc gatccatggg
      121 attgaatcaa acggccaatc cgtttttgcg tttccggagt ccatcagcgt attccactgg
      181 cacggggaga cgtttgattt gccgtcggga gcaacgcttc tggcaaaaag cgacgcctgc
      241 gaacatcagg cgtttcaggt cggcaagtcg gttatcggtc ttcagtttca cttggaaacg
      301 atgcccgcag cagcacaggc gcttgttacc aactgccgcg atgaattggt cccggcgccc
      361 ttcattcaga ccgaagaaga aatactgtcg acaactcctg atcaatacac agccatcaat
      421 gatctgatgg ccgacatttt atcttttctg cttacacaaa aaatgacggc aggactgtaa
      481 tcgagttgtc ctgtatcaaa tactcataat aaatatcttt acgtatttat gatcttgccc
      541 ggttcagcag cggcgataat ttcgcattct ttctgcattt ttttacaaag gtgtaatgat
      601 ttggcgcatt atttgcgata gagagatttt aaagaaaaaa cattggttga aatttgaaca
      661 attttgtaac aaaataaatt gctggcgaaa taaagatgac taaaacgtca aaaaaatctt
      721 tggaagagag agaaagtcaa ttgagtattc atacctatga ggaattggca gccctgcatg
      781 cgatagcgca aattttagcc caacccggag atttacacca tcagcttgca ctgattttaa
      841 aggaaatgag ttctcgtctc gggatgcagc gcgggatgat atcgctcgtt gaccgcgata
      901 ataaggaatc gtggctggat gtggcccatg atgtgaatat agatggcctt gagataacct
      961 ataaacatgg cgaaggcatt accggaaaag ttgcagaaac cggccggccc atggcggtgg
     1021 ccaatctggg tcaggaaacg catttcctgg accgcaccgg tgcccgccgt catttgaatc
     1081 gcgcggagct ttccttttta tgcgtgccga ttatatatga tttcatggtg gagggggttc
     1141 tctcggccga taaagtggcc gtcaaagtcg aaaatctgga taaggaactc gccttgatgg
     1201 catccgtcgc cgaactgatg gccaaggccg ttcatatccg cgctcttgag gaggaaaaca
     1261 aacgtttgcg taaaatcatc ggcagcaccc gagcgccttt gatggagatt atcggacatt
     1321 ccaaggcgat gcaggaagtc ttcgggctgg tggcgcaggt ggccgattcc aacaccaccg
     1381 ttttgatcac cggagaaacg ggaacgggca aggaattaat cgcccgtgcc attcataaaa
     1441 actcagtgcg taagaacggt cccatggtcc aggtcaattg cgcggctatt ccggataccc
     1501 tgattgaaag tgaattgttc ggtcatgaaa aaggggcgtt caccggagcc ttgacgcagc
     1561 gccgcgggcg ctttgaggaa gccaacggcg gcacaatctt tcttgacgag gtcggggaac
     1621 tttcagccgc cgcccaggtc aaactgctgc gggttttgca ggaaaaacgt tttcaaccgc
     1681 tggggtcgtc ccgtgtggtt aatgtcaatg tccggattat tgccgcgacc aaccgcaatc
     1741 tggagcagga tatcctttcc gaccgcttcc gggcggacct gttttaccgg ctgaatgtat
     1801 ttcccattta tttgcctcct cttcgcgagc gcggcagcga tgtgattatg ctggccgacc
     1861 actttcttct gaagtacagt aaagagatgg gcaaacaggt gaaacggatt tcccctgcgg
     1921 ctatcgaggt ttttctttcc cataaatggc cgggcaatgt ccgcgaactg gaaaactgta
     1981 ttgaacgggg tgttctgatg accaagacgg atgttctgga ttgcgagcat ctgcccccgt
     2041 ccctgcaatt gaaagacgga atgcatgatc gcaaaggtcg cggcaaactg tcttccgttg
     2101 tggaggctca ggagagattg ctgatcattg acgcgcttaa agaaacaagc ggtaatcaga
     2161 ccaaatcggc caaactgttg ggtacgacca agcggattat ccagtacaaa atttccaaga
     2221 tggggattga tcccaaaaat taccgtaaaa aaagctaaag atggccttcc cctgccatga
     2281 cctctctaca tttttcggtg caaatcgaac aaaaatgttt cacttttcta tctaagaaca
     2341 aaaatgtcac ttccattgtt ttatctgtac ggcaattctc cataaaaaat acctaaaagt
     2401 tgattaattc aggacatttt aattttcaac attgatggca cgactttagc agttagccaa
     2461 ggcggtccaa gtgtgctctt tttgtaccaa ggaggaaaga aaatatgtgt cgcctgtttg
     2521 gcatgacaag taatgatccg gtttccccgc tggcagccat tcgcgccatc gatgtgatga
     2581 gggaagggca tgacggttcg ggtgtcgggt tatttctgac ggatctggga agcgatttcc
     2641 aggaattcaa aaacgagccg attctttccg ggattttttc caatgaagga ctcaaaacgc
     2701 tcgaccgatt catgatcgat catgatttta tggtgaaata caaactttcc atcagaccgg
     2761 caaagaatcc tcccgcggga acgcccaaac gcgataatta cgttatccgg atttatgaat
     2821 atcccgagga gtgggacgga ttatgccggg acgaaatcga atttcggctg atgatggttc
     2881 aattggaatt gcggcggatg ggcgagcagg acgaatcgat gctggtgttc agtttctggc
     2941 ccgatgtgat cattatcaaa gaggttggag atccgctcgc tgttgccgaa tatctcggac
     3001 tggaccgcaa agagctgatg gcccgcgtta tcctgagcca gggacgacag aatacaaact
     3061 atgcgatcaa tatctatgcc tgccatccgt tttttattca ggggatggcg actgtgacaa
     3121 atggagaaaa caccgctttt gttccgatac gggaatttct gatgtcccgc aaattcccgg
     3181 gttataccgg ttatcattcg gactcggaaa cgttcacgca tattctgcat tacatgtatc
     3241 atcaattggg tatgggcatg gaaatgtaca agcatatcat tacaccgctt aaggacgggg
     3301 aactgaaccg tcatcccgac ggcgtgtggc tcagaaaatt gaaacagtcc tgccgcccgc
     3361 tgattattga cggtcccaat tgcgtcatcg gatgtctgcc ggataaatcg atgttcatgg
     3421 tgcaggattc gaaaaaactt cgtcctggcg tggtgggcgg tcgtcccggg gtgttcgcgt
     3481 tttcttcgga aatgtgcggc ctcgacgcgg cccttccgga cagatatgtt aacctggatt
     3541 ttcaacccat gaaatatgaa acagtgatcg ttcgcagcga acggcaggag atcgaaaaat
     3601 ggaatcagct ggaaccgtta acccatccac attaagcgtc aaagatctgc cctggcagat
     3661 catgtggaac aaagagaaat gcaccctgtg cggccggtgc accgcggtgt gtccggtgcg
     3721 tgcgatcgaa ccgggcgtgc atcgcaaacg cgcgctgcag gttcctgtcg gtctggaaga
     3781 aaaacccgcc cagttgttgt ccgtttattt cgggattgat cagcgcaccg atgcggcgca
     3841 tgcctgcgtc ggttgcggca tgtgcactct ggtttgtccc aacggcgcca tcatgccggt
     3901 gcgtgcggaa gaaacagaca aactgcggtt tcacgtcaac cagggcggcg agccccgcag
     3961 gcgcggcgga cggcgcaatg ttccggacag cgttctggat aaaataaaat ttatccggat
     4021 ttctatgctg accgatccgg cgctggatgc gggacgtcat gaatttgaac tcaggaccct
     4081 gctgggcaga gtgcttccgc cggaggagct tttgaaagtc catcgtgaaa acggatgggt
     4141 gccgccggtg cgtgaaatat atccgttggt tatcggcggg atgtctttcg gggcattgtc
     4201 cccgaatatg tgggaaggcc tgcagatggg ggtggcgtat cttaatgaag aactggggat
     4261 gcccgtgcgc atctgcaccg gagagggggg atgccctccg cgactcctga ggtcccgttt
     4321 tttgaaatat gtcattctgc aaattgccag tggctatttc gggtgggatg aaatcattca
     4381 tggtcttccg cagatgaaag aagatccctg cgcgattgaa ataaaatacg gccagggcgc
     4441 caagcccggc gacggcggat tgctgatgtg gtacaaggtc aataaactga tcgccgccat
     4501 ccgcggcgta ccgcagggcg tgagcctgcc cagcccgccg acacatcaga ccaagtactc
     4561 gatcgaggaa gccgtggcca agatgattca gtccatgtcc atggcctggg gtttccgcgt
     4621 gccggtctat ccgaaaatat ccgcgaccag cacgacgctt gccgtgctga ataatctgac
     4681 caggaaccct tatgccgccg cgctggccat tgacggagaa gacggcggaa caggcgctgc
     4741 ttataatgtc tccatgaatc atatggggca tccgatcgcc agcaatttgc gcgacgcgta
     4801 tctgaatttg gtcaagatcg gcaagcaaaa tgaagtccct ctgtttgccg gaggcggcat
     4861 cggcaagaac ggcaatctcg cggccaatgc cgccgccctg atcatgctgg gtgccagcgg
     4921 tgttcaaacc ggaaaatata tcatgcaggc ggcggccggt tgtctggggt ccgagacgga
     4981 ccgctgtaat atttgcaata tcggcgcctg tccgaaaggc attacctcgc aggattcgcg
     5041 actctatcgg agactggatc ccgaaaaggt cgctcaacgg ctcgtcgatg tatttttgag
     5101 ctttgatacg gagttgaaaa aaatcgtcgc accgctgggg cgttcgacat ctctgccgat
     5161 cggcatgtcc gatgcgctgg ggattgccga tgccggcgcg gctgagagat taaaaataaa
     5221 atacgttctg tagaatgaaa acagtgcagg gattatgaca aagaaagaat cgttaaaagt
     5281 aaccggccag gaaaacggaa tccgtgttga atcgcgaatt ctcgaagagc ggattcagca
     5341 ggctgtagcc gagggctatc gtcatattga gataacggct tacggccagc atggtatcgg
     5401 cggccgtctc tggcgggccg gagaaacgcc aatcaaggtg gatgtgacgg gaacatcggg
     5461 acagcgtctc ggttccatgg ggtttgccaa cacgctcatc gaggtgttcg gtccggtttc
     5521 cgatgacgtg ggctggctca atgccggagc gaaaatcatt gtccacggta atgccaccaa
     5581 cggggtggcc aacgccatgg cccaggggaa agtttttatt gacggcgata tcggcgcccg
     5641 gggcatgacc atgaccaaac acaatccgcg ttttgatccg ccggaactct gggtgttcgg
     5701 aaaagccggc gattcgttcg cggaattcat ggccggcggc atcgcggtca tctgcggtgt
     5761 cggcgctgaa aatgaggatg acgttttggg ttaccgtccc tgcgtgggga tggtgggagg
     5821 caagattttc ttccgcggcc gtcaaagctc ttacagcacc caggatgcgc gactcattac
     5881 cgatcttccg gatgaggaat ggcggtggct gacaaaaaat ctgaaagcct ttctgcagga
     5941 tgtcggcaaa caatacttat gtgaagaact gaccgcccgt cgttcggact ggaagcttct
     6001 gtccgctttg aaaccccacg agaagaaagg gcgtgccctg agtccgatga cggtttaccg
     6061 gaaagaggca tgggaaaagg aactgggcac cggcggtctg atcggtgact tgaccggcgc
     6121 cgaccagtcc ccgatcggtc tgatcacgac cggtgattta cgccgttttg ttcccgtctg
     6181 ggccaatgaa aaatatctgc cgccctgtca ggcgagctgt ccgacgggca ttcccgttca
     6241 aaaacgatgg gaattgatcc gcaaaggaaa aatgaaagac gcggtggatc ttgccctgcg
     6301 gtatacgcct ttcccggcga cggtttgcgg ttatctttgc cccaatcttt gcatgcagaa
     6361 ctgttcgcga aaaatcgtca gcctgccgtc ggtggatgta accgttctgg ggaaagcgtc
     6421 tctggaagca aaagaaccga aaccactgcc ggccaccggt aaaaaaatag cgatcatcgg
     6481 cggcggcgcc gcgggacttt ccgtggcctg gcagttatgg atgaacggtc atgacgcggt
     6541 gattttcgag agcagaaaac atctgggcgg taaaattacc caaaccattc cgcagagccg
     6601 tattcccgat aatgttgtgg aacatgagct caaacggctg gcgaaaaatc tgcgtcacat
     6661 tcatctgaac gcgtctttga cgaaagagaa gtttagaaaa ttgaagaatg aattcgactt
     6721 catcgtcatt gccgccggag cggcaaaacc ccggaaacta aatgtccccg gaatggaaaa
     6781 agcattgacc gcgctcgagt ttctgaaaca gagcaaaggc aacggcatca atgtcggaaa
     6841 gaaagtcgtc atcgttggcg ccggcaatgt cggatgtgac gccgcgacgg aagcttttcg
     6901 cctgggcgcc aaaaccgtta cgctgattga cgttcagcag ccggcatcct tcggcacgga
     6961 acggaaacat gccgaagcgg cgggtgcgat cttcctctgg ccccgcttta ccaaggccat
     7021 cacggccaga ggcgtggaac tgaccgacgg ggaaatcctg cctgcggata cggtcatcgt
     7081 ggctgtcggc gacgttccgg atatatcgtt tttgccggaa gaaatagccg tcaagaacgg
     7141 gttcatcgcg gtggacgaac aattcagaac aagcgacgat cagatatttg ccgtcggcga
     7201 tgtcaccaag cccggtttgc tcactgaagc catcggcgcg ggaagaatcg ccgccggaac
     7261 aattgacgca ttgctcagag gcgccggtga aacatatgac aaactgccgc cgatcgatgt
     7321 tcaacgggtg aagacagaat atttcgattc gcgcatcaag gctttttccg atcccgtttc
     7381 ctgcgccgtg aactgcgcgt cctgcggcgc ctgccgtgac tgcggtatgt gcgagaccat
     7441 ctgtccgcag aatgccattt cgcgcaaagc cctaaacggt gaaaattatg agtatgttgt
     7501 tgatgcggaa ctttgcatgg ggtgcggttt ttgcgccggc gtttgtccga ccggcgtctg
     7561 ggaaatcgtg gacaataaac cactcgagta aaaaaatcca atagcgagga taagaaagac
     7621 tatgaaaaaa acaaaaaaga ccactgcagc ggagcgggaa tacgttattg aaaaggagaa
     7681 attcgttccg gtttcaaagt atttcggcga ggataccttc aaccaaaaaa cgatgaaaga
     7741 gcgtctgccg aaagaaacct tcaaaaaact gatggacgcc atcaacgaag acaagcggct
     7801 ggatttaaag acagccgatg tcgtggctga ggccatgaag aaatgggctc tggaaaaagg
     7861 g
//
LOCUS       DAOSME010000082         7642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_10690_length_7642_cov_18.683801, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000082 DAOSME010000000
VERSION     DAOSME010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10690_length_7642_cov_18.683801"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLG98_09960"
     CDS             <1..1239
                     /locus_tag="PLG98_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE33627.1"
                     /translation="YRNILRNKRRSVVTMIAIAVGFTSISIYHGYINNAYEGLRWLAI
                     CGERLGHLRINKAGWEEQGKLEPHNYMFSKEQTDKIIQLVKEEKDVVLVTPQIQVMGT
                     VTNGLTSIIFIAQGVIPKDEEFITQFWFDVVGYVDEGYDAVIGEKLNESKTYGVLMAK
                     DMASYLKMKPGSDGIVMAQTLSGQMNAMDMQISGVYDTGNDFSNDKFMRFNFNFAQAL
                     LDTQSAERVVVMLKHWKDTEQMHDVIAKKLKNAGIDAEIRTWQDLSIAYNKMKSYLDT
                     IFIFLFFIVVTIVLVTIINTMSNTIVERTKEIGTLRALGMKRVGVAKLFALEGALLGV
                     FGSMAGVVLHTIEWSLIRLFPLHYTPPGLSTPVAMRVNMAPWALMLLIVCFIIISTIA
                     AVIPAKSAAKANIVDSLGHP"
     gene            1299..2537
                     /locus_tag="PLG98_09965"
     CDS             1299..2537
                     /locus_tag="PLG98_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE33628.1"
                     /translation="MMNWMKLAFRNILRNKRRSFVTMVAIGVGFAAIGLYHGYIHNAY
                     AGLRVIAIRGEGLGHLRINKAGWNAKGKLEPEKYMFTREETEKIMKLVGEEKDVVLST
                     PQMQVVGTVTNGATSTVFIAQGVVPKDEVIIKGDWVVFKPRAGHKISDDVLYGVEMAK
                     DLAAYLNMNAGSNGIVMAQTLAGQVNALDMQVSGVYDTGNDFSNDKFMRFNFKFAQSL
                     LDTQSAERVVVLLKEWEDTEMMRDRLLKKLKAAGIDCEIRTWKELSLGYLKMKSYLDM
                     IFIFIASIVLGIVIMTTINTMGMAILERTKEIGTLRALGLKVKGVSLLFTLEGALLGF
                     LGSIVGILLHIVVWVIIRLFPPHYVPPGFSEPVPMFIDMVPWMLLLLVFCFIILSMIA
                     AIIPARGAARKNIVDALGHV"
     gene            2559..3332
                     /locus_tag="PLG98_09970"
     CDS             2559..3332
                     /locus_tag="PLG98_09970"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOE33629.1"
                     /translation="MMSRFLKKNYFTLWSFVVLFFLNVAPLQAMTNQEMLEQADKARG
                     NEEGIQWEIKMHSVEKGRVQDRTLLVIAKGNSASGYNSLIDTLEPGNLKGQKLLMQDR
                     NMWFAKPGLSKPVPISPRQKLMGDASNGDIAATNYAGDYKIIGSEDGQFNNEDCVVLN
                     LQAVDKRATYDRIKYWISKKRVLGVKAEFYTISGKMFKTAIFEYKNRITVEGKSQEFI
                     SQMTITNAIMKDDITVVKYSRPVLKKVSDATFNLNLLTK"
     gene            3342..>4759
                     /locus_tag="PLG98_09975"
     CDS             3342..>4759
                     /locus_tag="PLG98_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33630.1"
                     /translation="MKKKHNTIIRGAGIFLIILFCSWLPSAVLAAEDPFASFLQSNVK
                     QYTATDAEYADRPYLRKLQGAFNADFRILTYGVLQDPAKSTQNPNNDPVGFPNYIGNI
                     DVRPDFRFNTKYLDLVFKPRAMFDYRYWKEGQREGEDDWNDEWYVNEWLVRLKAAERV
                     FLSYGRENLQWGPSFLYSPSNPFFFDNGRSNPQMEVAGMDFARVVFVPHMLWTVSFMV
                     NTDQGRNTLISTDPFEETYAAKIDYTGNQNYASLILSHKDDDTTLGYFGGWTVSDALL
                     LYSEGSMRQGSDALYPNLKRRSLGQSFLEALFAPNSELRPILDRYTSLRSAYMDKTRE
                     DDSKIYPTILVGGAYTFEDIGTLTLEYMYHAQGYDEDEADLYYSLRGRAAYAYLDMGA
                     YPGLLGQFTLYQTGNTGLRFLRKNYTMLQYYKGNIFNRMETTLRWTQNVDDGSAQFLG
                     LLSYSLGNHVELFSSGVLNTGK"
     assembly_gap    4760..4859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4860..5973
                     /locus_tag="PLG98_09980"
     CDS             <4860..5973
                     /locus_tag="PLG98_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33631.1"
                     /translation="KNKNRDQAPDQEQKNPVRYALPVKRPLGTILVDGKFIKPETLDA
                     ALDQQKQTRDLLGNILVNMGMLDPADLNAALMVQRDFSTIEDAINAAAGVRMMLGELL
                     LASSRITRDQIEKALEEQKRTGEKIGEILVRLGLFSSTEMDIALSFQSRQNAGYASCL
                     SLGELLIAAGYITHDDLEDALERQKGNNRKLGEILVDAGRCKPHHVEHGLRMQKQLVA
                     AALIAALSLAPVKEAVSSVNPSSTAMTTNIKVTATVLARASMHVLRQPNEIVVTDADI
                     KRGYLDISAGSLVEIKNNSRAGVDLTFETHGLPFSEVLVSGFGREVALGPNGGIITHQ
                     LLGANIIAFSYRFVFDRNSRAGTYAWPLVLSVSPVE"
     gene            6063..>7642
                     /locus_tag="PLG98_09985"
     CDS             6063..>7642
                     /locus_tag="PLG98_09985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE33632.1"
                     /translation="MKKVKAESSLKNENKQQFFELDRLILRLSRQFAGSSATEIEEKI
                     GLALQQIVECLDIGRCTFWEVSEDETELRCIRQYVVPKLEKVYGVTPQQSPWISKDQW
                     NGNCSCNVRNEDSVSGAKANKDALLLLGGKSFIISPFHIAGKAVAGMTFSTLEEDREF
                     SPELVSRLNLIGEIITGAFYRKKIEEKYQQSIGDMRQQSGPARAEDFPLREDTKKEFR
                     YQGIIGNSEAMQNVFIKMEQVAPTDSVVLILGETGTGKGVLARTIHELSNRNKKRIVT
                     VNCAALTPNLIESELFGREKGAYTGSTEMQIGRFELADKGTLVLDEFTELPLDLQAKL
                     LRAIQEGEFERLGSPRTIRVDVRILALTSRNLKEEVSKGRFRQDLYYRLDVFPITMPP
                     LRERFGDIPLLVEHFVEQFNRKMQKEIKNIPGGVMAQLMKYPWPGNVRELEHIIERSV
                     IVTRGTSLQLAEKLEDQTLGLMEENAVTNLAEVEKRHILKILEKTNWRIDGSKGAAVL
                     LGLHPNTLRGRMQKLGIRL"
BASE COUNT         2004 a         1687 c         1991 g         1860 t
ORIGIN      
        1 taccggaata ttttaagaaa caaacgccgc tctgttgtga cgatgattgc cattgccgtg
       61 gggtttacct ccatcagtat ttatcacggc tatattaata acgcttatga gggacttcgg
      121 tggcttgcga tatgcggcga acggttgggc catttaagaa tcaataaagc gggatgggag
      181 gaacagggca agctagaacc gcacaactat atgttttcga aagagcagac ggataaaatt
      241 attcaactgg tcaaggaaga aaaagatgtc gtgctggtta caccgcagat tcaggtgatg
      301 ggcacggtaa ccaacggtct gacctccatt attttcattg cccagggcgt tatcccgaaa
      361 gatgaggaat ttattacaca gttctggttt gatgtcgtcg gttatgtgga cgaggggtat
      421 gatgcggtca tcggtgaaaa actgaacgaa agtaaaactt acggtgtgct gatggccaaa
      481 gacatggcca gttatctaaa gatgaaaccg ggcagcgacg gaatcgtcat ggcgcaaacc
      541 ttgagcggtc agatgaatgc catggatatg caaatcagcg gcgtctatga taccggaaac
      601 gatttctcca atgacaaatt tatgcggttt aattttaatt tcgcccaggc cctgctcgat
      661 acgcaatcgg cggagagggt tgtggtcatg ctcaaacact ggaaagatac ggaacaaatg
      721 catgatgtca tcgcaaaaaa acttaaaaac gccggtattg atgctgaaat cagaacctgg
      781 caggatcttt ccatcgctta taataaaatg aaatcgtatc tggatacgat ctttattttt
      841 ctttttttca tcgtggtgac gatcgtgctg gtgacgatta tcaataccat gagcaatacc
      901 attgttgaaa gaacgaaaga aatcggaacc ctgcgggctt tggggatgaa acgcgttggc
      961 gtggctaagc tttttgctct ggaaggcgct ttgctgggcg ttttcggcag tatggcaggc
     1021 gttgtcttgc atacgattga atggtcgctg atcaggctct tcccgctgca ctatacacct
     1081 ccgggattgt caacgcccgt ggccatgaga gtcaatatgg cgccctgggc gttgatgctc
     1141 ttgatagtct gtttcatcat catatcgacc attgcagccg tcattcccgc aaaatcagcg
     1201 gcaaaagcga atattgtaga ttcactggga catccctgaa atatttttgg attaaaaaga
     1261 taaactgaac atttctgaaa ggcggctgga ttaaacctat gatgaactgg atgaaactgg
     1321 cattccggaa tattttaaga aacaagcggc gatcctttgt gaccatggtg gccatcggag
     1381 tagggtttgc cgcgatcggc ctttaccacg gctacattca caatgcttat gccggactgc
     1441 gggttattgc gattcgcggc gaaggacttg gccatttgag aatcaacaag gccgggtgga
     1501 acgccaaggg caagctggaa cctgaaaaat acatgtttac ccgggaagaa accgaaaaga
     1561 tcatgaaact tgtcggagag gaaaaagatg tcgtcttgtc cacaccgcaa atgcaggtcg
     1621 tcggaacggt gacaaacggg gcaacgtcta cggttttcat cgctcagggg gttgtgccca
     1681 aagacgaagt catcatcaaa ggggattggg tggtttttaa gccgcgcgcg gggcacaaaa
     1741 taagcgacga cgtactctac ggagttgaaa tggccaagga cctggctgcc tatctgaaca
     1801 tgaatgcggg cagcaacggg attgtcatgg cacagacgct ggccggtcag gtgaacgctt
     1861 tggatatgca ggtcagcggt gtctacgata ccggcaatga tttttccaat gacaaattta
     1921 tgcgcttcaa ttttaaattt gcgcagtctc tgctggatac gcaatcagcg gaacgcgtgg
     1981 tggttctgct gaaagaatgg gaagacactg aaatgatgcg cgatcgtctg ctcaagaagc
     2041 tgaaggccgc cggcattgat tgtgaaatca gaacctggaa agaactctct ttgggttatt
     2101 taaaaatgaa gtcctatctg gacatgatat ttatttttat tgcttccatt gtgctgggga
     2161 tagtgataat gacaaccatc aataccatgg ggatggccat tctggagagg accaaagaga
     2221 tcggaaccct gcgggcgctg ggactcaaag tcaagggtgt ttccttgctt ttcactctgg
     2281 agggagcgtt gctcggattt ctgggcagta tcgtgggtat tttgctgcac attgttgtgt
     2341 gggtcattat ccggctgttt ccgcctcatt atgttcctcc cggcttttcg gagccggtgc
     2401 ccatgtttat tgatatggtg ccgtggatgt tgctgctgtt ggtattttgt ttcataatat
     2461 tgtccatgat tgcagcgatc attcctgcaa gaggtgcggc caggaagaat attgtggacg
     2521 cgctgggaca tgtataaaaa ttatagatca gggagaaaat gatgagtcga tttttaaaga
     2581 aaaactattt taccctatgg agttttgttg tattattctt tctcaatgtt gcgccgttgc
     2641 aggcgatgac caatcaggaa atgctggaac aggccgacaa ggcgcggggc aacgaggaag
     2701 gcattcagtg ggaaattaaa atgcattccg tggaaaaagg acgtgtgcag gatagaacct
     2761 tgctggttat tgccaaagga aacagcgcca gcggttataa ttccctcatt gatacgctgg
     2821 aacccggcaa tctgaagggg caaaagctgc tgatgcagga tcgcaatatg tggtttgcaa
     2881 aaccgggtct gtccaaaccc gttcctattt caccgcggca gaaactgatg ggcgatgcgt
     2941 ccaacggcga tattgcggcc acaaactatg cgggtgatta taaaattatc ggatcggaag
     3001 acgggcagtt taacaacgaa gattgcgtcg ttttgaattt acaggcagtg gacaagagag
     3061 ccacgtatga ccggatcaag tactggatat ccaaaaaaag ggttttgggc gtgaaagctg
     3121 aattctacac gatttccgga aagatgttta agacggcgat attcgagtat aaaaacagaa
     3181 ttacggtgga gggtaagtct caggaattca tttcccagat gaccatcacc aatgcgatca
     3241 tgaaggatga tatcacggtc gtgaaataca gccggccggt gctgaaaaaa gtttccgatg
     3301 caacatttaa tttaaattta ctgacaaaat agaggttatt tatgaaaaaa aagcacaata
     3361 ccataatcag gggagcgggt atttttctca tcatcctctt ttgttcatgg ttgccttcag
     3421 cggtgctggc cgccgaagat cctttcgcct cttttctgca gtcaaacgtc aaacagtaca
     3481 cggcgaccga tgccgagtat gcggaccggc cttacctgag gaaattacag ggcgctttta
     3541 atgcggattt ccggatttta acgtacgggg ttcttcagga tcccgccaaa tcaacgcaaa
     3601 acccgaacaa cgatccggtt ggatttccca attacatcgg gaatattgat gtcagaccgg
     3661 atttccgttt caataccaaa taccttgatc tggttttcaa gccgcgggcg atgtttgact
     3721 atcgctactg gaaagaaggc cagcgtgaag gtgaggatga ctggaacgat gaatggtatg
     3781 tcaatgaatg gctggtcaga ttgaaggccg cagagagagt cttcttgtcc tacggacggg
     3841 aaaacctgca atggggacct tctttccttt attctccctc gaatccgttt ttctttgata
     3901 acggccgcag caacccccag atggaagttg ccggaatgga ttttgcccgt gtcgtgtttg
     3961 ttccccacat gctctggacg gtctctttca tggtcaatac ggatcaggga cgtaatactc
     4021 ttatcagcac cgatcctttt gaggaaacat atgccgctaa aatagattac acgggaaacc
     4081 agaattacgc ttctttgatt ttatctcaca aggatgatga tacgaccttg gggtatttcg
     4141 gcggctggac ggtttccgac gccctgttgc tttacagtga aggcagcatg cgtcagggaa
     4201 gcgatgcttt gtatccgaat cttaagagaa gatcactcgg tcagtcgttc ctggaagctt
     4261 tgtttgcgcc gaattccgaa ttgaggccga ttctggacag atacacctcg cttcggtcgg
     4321 cgtatatgga taaaacacgg gaggatgact ctaagattta tccgacaatt ctggtcggcg
     4381 gagcctatac gtttgaagat atcggtaccc tcaccctgga atacatgtat catgcgcaag
     4441 gttatgatga agatgaagcg gatctctatt actcgctcag aggccgggcc gcctatgcgt
     4501 atcttgacat gggagcctat ccgggactgc tgggccagtt tacgctgtat cagaccggca
     4561 atacgggctt aagatttttg cgtaaaaatt acacaatgct tcagtattac aagggaaata
     4621 ttttcaatag aatggaaact acgctgcgct ggacacagaa tgtggatgac gggtcggctc
     4681 agtttctggg acttttatcc tattccctcg gcaatcatgt cgaattgttt tcatccggtg
     4741 tgctgaatac cggaaaagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4861 aaaaataaaa accgggatca ggctccggac caggaacaaa aaaatcctgt ccggtatgct
     4921 cttcccgtga aaaggccgct cgggaccatt ctggtggacg gcaaattcat caagccggaa
     4981 accctggatg ccgctcttga tcaacagaaa caaacccggg accttctggg aaacattctc
     5041 gtgaacatgg gcatgctgga tcctgccgac ctgaatgccg ctctgatggt tcagcgtgat
     5101 ttttcgacca ttgaagatgc gataaacgct gccgcaggcg tccggatgat gctgggagaa
     5161 ctgcttctgg cctcatcgcg cattacccgc gatcagatcg agaaagcgct tgaagagcaa
     5221 aaaagaacag gcgaaaaaat aggagaaatt cttgtaaggc tcgggctttt ctcgtcaacg
     5281 gagatggata tcgcgctgtc gtttcaatcc cgtcagaacg cggggtatgc gtcctgcctg
     5341 tccctgggcg aactcctgat cgctgcagga tacatcacgc acgatgatct tgaagacgct
     5401 ctggagcgtc aaaagggaaa caacaggaaa ctgggtgaaa ttcttgttga tgccggccga
     5461 tgtaaaccgc atcacgtcga gcacggtctg cgaatgcaaa agcaactggt tgctgctgcg
     5521 ctcattgccg ctttatccct ggcgccggtc aaggaagccg tatcctcggt aaatccgtct
     5581 tccacggcca tgaccaccaa tatcaaagtg actgcaaccg tgctggcgcg tgcgagcatg
     5641 catgttcttc gtcaaccgaa cgaaattgtg gtgacggatg ccgatattaa acggggatac
     5701 ctcgacatca gcgccggctc tctggtcgaa ataaaaaaca attcccgagc gggcgtcgat
     5761 ctgacttttg aaactcatgg cctgccgttt tcagaagttc tggtcagcgg ctttggccgg
     5821 gaagtggcac tgggacccaa cgggggaatc ataacccacc agctcctcgg cgccaacatc
     5881 attgcgttca gctatcgttt tgttttcgac agaaactcgc gggccggaac gtatgcctgg
     5941 cctctggtcc tctcggtgag tcctgtcgaa taaaagatag ggttgaattg cagcggcttg
     6001 gacgtatcat tcagacgggg aaaaagccga ttttaacatg acagcctttt tgcaatcata
     6061 aaatgaaaaa agtgaaagcg gaatcttcct taaagaatga aaataagcaa cagttcttcg
     6121 aactcgacag actgattctg agactttcca ggcagtttgc cggatcttcc gcaactgaaa
     6181 tcgaggaaaa aatagggctg gcattgcagc aaatcgtcga atgtctggat atcggccgct
     6241 gcacattttg ggaagtatcg gaagatgaaa ccgaattgcg ctgtatccgc cagtatgttg
     6301 ttccaaaact ggagaaggtg tacggcgtta cccctcagca atccccatgg atttccaaag
     6361 atcaatggaa cggcaattgt tcgtgcaatg tccgtaacga agattcagtc tcgggagcga
     6421 aagccaacaa ggacgccttg ttgctgttgg ggggcaagtc tttcattatc agcccctttc
     6481 atatcgccgg caaagccgtt gccggcatga cgttttccac cctggaagag gacagggaat
     6541 tttcaccgga gcttgtttcc cggctgaatt tgatcgggga aatcatcacc ggcgcttttt
     6601 accggaaaaa aatcgaggaa aagtatcagc agtcgattgg cgacatgcgt cagcagagcg
     6661 gtccggcccg ggcagaggat ttcccgctgc gggaagacac gaaaaaagaa ttcagatatc
     6721 agggcatcat cggtaattcc gaagcaatgc aaaacgtttt tatcaaaatg gagcaggttg
     6781 cgccgaccga ttccgttgtt ctcatcctcg gcgaaacggg aacgggaaaa ggcgttttgg
     6841 cccgtaccat ccatgaactc agcaacagaa acaaaaaaag aatcgtaacc gttaattgtg
     6901 ccgctctcac gcccaatctg atcgaaagcg aactattcgg ccgggaaaag ggcgcctata
     6961 ccggatccac cgaaatgcag atcggccgct ttgagctggc cgacaaggga acgctggttc
     7021 tcgatgagtt tacggaactt ccccttgatc tgcaggccaa acttctccgg gccatccagg
     7081 aaggggaatt cgaacggctt ggttctcccc ggaccatccg ggttgatgtg cgcattcttg
     7141 ctctgaccag caggaatctg aaggaagaag tttccaaagg acggtttcgc caggaccttt
     7201 actatcgttt ggacgttttt cccatcacca tgccgcctct ccgggaaaga ttcggcgata
     7261 ttccgttgct ggtggagcat tttgtggaac agtttaaccg gaaaatgcag aaagaaatta
     7321 aaaacattcc cggaggggtt atggcgcaac tgatgaagta tccgtggccg ggcaatgtcc
     7381 gggaactgga gcatatcatt gagcgttccg ttattgttac gcgcggaacc agccttcagc
     7441 ttgcggaaaa gctggaggat cagaccctag gtctcatgga agaaaatgca gtcacgaatc
     7501 ttgccgaagt tgaaaaacgg cacatcctta aaatcctgga aaagaccaac tggcggatcg
     7561 acggttccaa aggcgccgct gttctgcttg gtttgcatcc caatacgctg cgcggacgaa
     7621 tgcagaaact gggcatccgg ct
//
LOCUS       DAOSME010000083         7568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_10818_length_7568_cov_19.907361, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000083 DAOSME010000000
VERSION     DAOSME010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10818_length_7568_cov_19.907361"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..839
                     /gene="rnc"
                     /locus_tag="PLG98_09990"
     CDS             120..839
                     /gene="rnc"
                     /locus_tag="PLG98_09990"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOE33633.1"
                     /translation="MDKSRREVLKKLEERLDYRFRDVDLLVTALTHRSYVNENRQPGV
                     SDNERLEFLGDSVLGLCVSDLLIKKYVDGSEGDLTKMRSVLVGEKNLAQLARGLQIGN
                     CLLIGRGEENAGSRARDSFLANAFEAVVAAVYLDSGYENVRAVVRKLIEPFLEKETLP
                     SAYFDYKTLLQEFCQRKFKTIPMYLLADTSGPEHAKVFEVKVVIVDKLTAIGTGTSKK
                     EAEKQAAQKAWKILQDDESQL"
     gene            820..1929
                     /locus_tag="PLG98_09995"
     CDS             820..1929
                     /locus_tag="PLG98_09995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33634.1"
                     /translation="MTKASYRKPNDNYPLIIPVFIMNSGCPHRCVFCNQKISAGNFPE
                     KITQEYFDRTVTSYLEWNKDKTRRVEIAFYGGSFTGIDSGDQEALLAMADAFIQRGLV
                     HSIRISTRPDYIFEKQLSMLKKYNVATVEIGAESFVDDVLRYAGRGHCAADTEKAVMM
                     LKKYGFKTGLHLMAGLPGDTAEGFDYSLDRTIELSPDTARIHPVLVFAGTALAEEFRK
                     GAYRPLDIASAVELCRRAWEKLAAAGIRIIRMGVQTTPEMEKQGTVLAGPVHPAFGSL
                     VRSSVFYHSTVNVLEKISGDVQEIRFYLSERDLSIFRGLNNINTEAIKKLYPRAKLII
                     ESQSDRPRGELSVAVNSGPLLHWTIPGFHQSVRGQ"
     gene            1935..2825
                     /gene="era"
                     /locus_tag="PLG98_10000"
     CDS             1935..2825
                     /gene="era"
                     /locus_tag="PLG98_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOE33635.1"
                     /translation="MFKSGFIGIIGRPNAGKSTLFNAIVGDKISIMTHKPQTTRNKIT
                     GIKNYPDAQLIFLDTPGMHKPKTPLNRAMVRTTREAIGSVDVLLMMIEAQTDVQPHDL
                     FLIESLNETKAPVLLVINKIDLIEKKFLLPLMDKFRRIYDFREIVPISALHGNGVEEL
                     LTIVKEMLPEGPKYYPDDIVTDATERFIASEFIREKITLHTKQELPYSAAVEIDLFKE
                     DEARNLIRITATINVEKDSQKAIMIGKKGAMLKKIGTQARLDMENLFGAKVYLELFVR
                     VRKEWTASERMLEEFGLLKH"
     gene            2833..4152
                     /gene="der"
                     /locus_tag="PLG98_10005"
     CDS             2833..4152
                     /gene="der"
                     /locus_tag="PLG98_10005"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOE33636.1"
                     /translation="MKIKPVIAIVGRPNVGKSTLFNRIAGKQKAIVIDEPGATRDRNY
                     MDCAYHDRAFTLIDTGGFEPASEEKILVQMREQSNLAIEEADVIIFMMDGKDGLTASD
                     IEIARQLRGKGKKVFYIANKVDGERYKAMLSDFYRLGIDEIYPVSAQHNLGVDELMNA
                     MVHDFPESSEEKRENDEQIKVAIVGKPNVGKSSLVNKILGKERTIANPIAGTTRDAID
                     STVKIHGKKYLIIDTAGIRRKSKISQTLEKYSVVQALKSINRCDIVLVLIDAEEGITE
                     QDTKITGLAYERGKACLIVVNKWDKIKKDNATVGKYVEEIKDKLKFLDFAPIIFISAV
                     TGQRAPKIFDLIDKVYAQYTQRIGTSPLNNLVEQAVRKNPLPRYQNRLMQIYYATQTD
                     IKPPTFVFFVSNSKGIHFSYERYLMNQIREKFEFDSVPLKLIFRKKS"
     assembly_gap    4683..4782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4783..5571
                     /locus_tag="PLG98_10010"
     CDS             <4783..5571
                     /locus_tag="PLG98_10010"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE33637.1"
                     /translation="GVRLDISGAVPENARTCLIVWPCMGGAVQMYRVPVEKFTDQGCA
                     CILYNPRGHGRSEGELEIQTALNDLDEILQENVPSAIPLVIVGHSAGANAGLQFGVRC
                     LPPKQFVLIAPVLDSRESLFYMYQRKTIDEFVNILCAYVQDDALVRKVLVDDQWLDAD
                     LWKARHYRHLLDRVHARMRIGTFLERLFIPGHNAFAQLERFSAQTRIFMAGEDTWYPA
                     ATTRRLADKYGIPVETLPEAQNHFFTGAWDSMWKKILDLILLPG"
     gene            5797..>7568
                     /locus_tag="PLG98_10015"
     CDS             5797..>7568
                     /locus_tag="PLG98_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOE33638.1"
                     /translation="MIYFHNISLSFLDKKLFDKMNWTIHPKSRIGLVGENGTGKTTLF
                     RMIMDDVRPDDGTLDIPGKKQKIFGYLPQDLVELESIPLIDYLKQKSGMADLETSIKD
                     LEQKLSKIKHDTAEYRELSQKYSDATALFHARDGYAFESRAKQMLKGFGFKESDFKQN
                     CDSFSGGWKMRILLTSILLAKPDIMLLDEPTNHLDTESMEWLESYLKDFRGTMIVISH
                     DHFFLDKVTTQIAELSSCAIHLYKGNYTHYLTEKEKRQDALKKEMELQKSQIKKIEEF
                     VERFRYKASKAAQVQSRIKMLEKFSEVTPEGKTKNVAMKFPEGKKSAKEVVTVTGLGH
                     RYGDLTVFGDLNFALFRGDKVAFVGMNGSGKSTLSRLLGKMENPSSGVVKYGDGVNMA
                     FYSQESSQNLNYEKTILEEINAVPTDASDQEKRNLLGTFLFSGDDIFKPVSVLSGGEK
                     SRLALLKIMLLDTNFLILDEPTNHLDIKTKDIFQNALIHYRGTVAIVSHDRYFLDHLV
                     NKVFELKDGAIHYYNGNYSYFIEQRSRPADAAGDEKPAQGVIRNQIVNAKEKKRLEAE
                     ERNKTYKIRAALKKELSDVEKKISG"
BASE COUNT         2144 a         1606 c         1874 g         1844 t
ORIGIN      
        1 ggggggcggg caaattgcct attgcgcata ttcgattttt accttattga agatttcagc
       61 aaaggtgcat catgttggga cgaaccaaaa tgtttacata aaaaatagga catatcgcta
      121 tggataaaag cagaagagag gttctgaaaa aactggaaga gagacttgat tatcgtttcc
      181 gcgatgtcga tcttcttgta accgcgctga cccatcgttc ttatgtcaat gagaaccgtc
      241 agccgggcgt gtccgataat gaacggctgg aatttctggg agattccgtt ttaggattat
      301 gcgtgagtga tttgctcatc aaaaaatatg tcgatggttc cgaaggcgat ctgacgaaaa
      361 tgagatcggt tctggtgggc gagaagaatc tggctcaact cgcacggggg ctgcaaatag
      421 gcaattgtct tttaatcggg cgcggtgaag agaatgccgg cagccgcgcc agggattctt
      481 tcctggccaa tgcgttcgag gccgtcgtgg ctgccgttta tctggattcc ggttacgaga
      541 atgtgcgggc ggttgtgaga aaattaatcg agccctttct ggagaaagag actctaccgt
      601 ccgcttattt cgattataaa accctgctgc aggagttttg ccagagaaaa ttcaaaacaa
      661 ttccaatgta tttgcttgcc gatacatccg gtccggagca tgccaaggtt tttgaggtaa
      721 aagtggtaat tgtcgacaaa ctgacggcaa tcggtacagg gacgagcaaa aaggaagcgg
      781 aaaaacaagc cgcgcaaaag gcctggaaaa tactgcagga tgacgaaagc cagttataga
      841 aaaccgaacg ataactatcc gttgattatt cccgttttta tcatgaacag cggttgtccg
      901 catcgctgcg tcttctgcaa tcagaagatc agcgccggga atttcccgga gaaaataacc
      961 caggagtatt ttgacaggac cgttacctct tatctggagt ggaacaaaga caagaccaga
     1021 cgagtggaaa tcgcctttta cggcggaagc tttaccggaa tcgattcggg tgatcaggaa
     1081 gcactgttgg cgatggctga tgcgtttatc caacgaggtc tggttcattc catccgaatt
     1141 tcaaccaggc cggattatat ttttgaaaaa caattgtcca tgttgaaaaa atataacgtt
     1201 gccaccgtag agatcggtgc ggaatctttt gttgatgatg tgttgcgcta tgccggaaga
     1261 ggccactgcg cggcagatac ggaaaaggcc gttatgatgt tgaaaaaata cggattcaaa
     1321 accgggcttc atctcatggc cggtctgccg ggtgacacgg ctgaaggctt tgattattct
     1381 ctggacagaa ccatcgaact ttctcccgac accgcaagga tacatcccgt tcttgtcttt
     1441 gcaggaaccg ccctggctga ggaattccga aagggggcgt accggcccct ggacattgcc
     1501 tctgccgtgg agttatgccg cagggcctgg gaaaaactag ccgctgccgg gatccgaatc
     1561 attagaatgg gcgtgcagac cacacccgag atggaaaaac aaggaacggt tctggccgga
     1621 ccggtgcatc cggctttcgg aagtctggtg cggtcctctg tcttttatca cagcaccgtt
     1681 aatgttctgg aaaagatttc cggggatgtc caggaaatac gtttttattt atcggagcgg
     1741 gatctgtcca tttttcgagg attaaacaat atcaatacgg aggcgataaa aaagctttac
     1801 ccgcgtgcga aattaattat agaatcacaa tccgaccggc cgcggggtga actctccgtc
     1861 gccgtaaatt cgggaccgtt gttgcattgg acgattccgg gatttcatca atcggtgaga
     1921 ggacaataaa gatcatgttt aaatcgggat tcatcggcat catcggacgt ccgaatgcgg
     1981 gcaaatcaac gcttttcaac gccattgtcg gtgataaaat atccattatg acccataagc
     2041 cgcagacaac gaggaataaa ataaccggga taaaaaatta tccggatgcc cagttgattt
     2101 ttctcgatac gcccggaatg cataaaccga aaacaccgct gaaccgtgcc atggtgcgga
     2161 cgaccagaga ggcaatcggc agcgttgatg tcctcttgat gatgattgaa gcccagaccg
     2221 atgtccagcc ccatgatctt ttcctgatag aatccttgaa cgagaccaaa gcgccggtgc
     2281 tgctggtaat caataaaatc gatttgattg aaaaaaagtt tctgctgccg ctaatggata
     2341 agtttcgaag gatttacgat ttccgggaaa tagtcccgat atccgcattg cacggcaacg
     2401 gcgttgaaga attgctgacg attgtcaagg agatgcttcc ggaggggccg aaatattatc
     2461 cggacgatat tgtgacggat gcgaccgagc gatttattgc ttcagaattt atccgggaaa
     2521 aaattacgct tcataccaaa caggagcttc cttattccgc agccgtggaa attgatttgt
     2581 tcaaggagga tgaagccagg aatcttatcc ggatcactgc gacgatcaat gtggaaaaag
     2641 attcgcagaa agcgatcatg atcggcaaaa aaggcgccat gcttaagaaa atcggcaccc
     2701 aggcgagact ggatatggaa aatctcttcg gtgccaaagt gtatctggag ttgtttgtca
     2761 gggtaagaaa agaatggacc gcttcggaaa gaatgcttga ggaattcggt ttactgaaac
     2821 attagaggct gtatgaaaat caaaccggtc atcgcgattg tcggacgtcc caatgtcggc
     2881 aaatccaccc tgttcaacag aatagccgga aaacaaaagg ccatcgtgat cgacgagccg
     2941 ggggcgacac gcgaccgtaa ttacatggat tgtgcatatc acgacagggc ctttacgctg
     3001 attgataccg gaggatttga accggcctcc gaagaaaaaa ttcttgtcca gatgcgcgag
     3061 cagagcaatc tggccattga agaggccgac gttatcattt ttatgatgga tggaaaggat
     3121 ggtcttacgg cgtctgatat agaaatcgcc cggcagttgc gagggaaggg gaaaaaggtt
     3181 ttttatatcg ccaacaaagt tgacggagaa cgttacaagg caatgctttc cgatttctac
     3241 cgtttgggaa tcgatgagat ttaccctgta tccgcgcagc acaacctggg tgtggacgaa
     3301 ctgatgaatg ccatggtgca tgatttccct gaatcgtccg aagaaaaacg tgaaaatgac
     3361 gaacagatca aggttgccat tgtcggcaag cccaatgtgg ggaaatcatc gctggtgaac
     3421 aaaatactgg gcaaggaaag aaccattgcc aacccgattg ccggaaccac acgcgatgcc
     3481 atcgattcaa ccgtcaagat tcacgggaaa aaatacctga tcatcgatac agcaggcatt
     3541 cgccgaaaga gtaaaatcag tcagacactg gaaaaataca gtgtggttca ggcgctcaaa
     3601 tccatcaatc ggtgcgacat cgtgcttgtg ctgatcgacg ccgaagaagg cattaccgaa
     3661 caggacacaa aaattacggg gctggcctac gaacgcggga aagcctgcct gatcgtcgtc
     3721 aataaatggg ataaaataaa gaaggataat gcaaccgttg gaaaatacgt cgaagaaatc
     3781 aaggataaac tcaaattcct ggattttgca ccgattattt ttatttcggc ggtaaccgga
     3841 cagcgtgcgc cgaagatttt cgatctgatt gataaggtgt atgcccaata tacgcaaaga
     3901 atcggcacat cgcctttaaa taatctggtc gagcaggccg ttcgaaaaaa tcctctcccg
     3961 cgctatcaga acaggctgat gcaaatttat tacgcaaccc agaccgatat taagccgccc
     4021 acgtttgtct tctttgtcag taacagcaaa ggaatccatt tctcctacga gcgctatctg
     4081 atgaaccaga ttcgtgaaaa attcgaattt gacagcgtgc cgctgaagct tattttccga
     4141 aaaaaaagtt agagggattc cggatttcgc gcttaatgat attattttaa gcaaatgatc
     4201 atcggcggca tttaaaattc tcctgcacct tgaacggttt tttgcgcata tggttgcgaa
     4261 gggcttcccg gcgttagaaa gaatgaattc aacgtggtct ttccagaggc aagtgaaata
     4321 tttttgacaa tcatcaatac gctttagatt ttttatccga attgtgatat gaaatgttcg
     4381 taaaattcat tatggagttg cataaaaacg atttgattat tggtcattca aaaattattt
     4441 tctaagaggg aatcgttatg aaaacatgtc ttcattgcgg aaagcaactg cgggatgaaa
     4501 cgacgaaatg ttttttctgt ggccaaatgg cggatgaatt gccgcaacag aaaaaaggtt
     4561 ttgagatgtc gaaaaagaac agggatatcg ctctattaat tatcgtccta attatctgtc
     4621 tcgttattta tatattaaaa aaataattat ttaagaatgt tgtattagaa cgatttgcga
     4681 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcgttcg cctggacata
     4801 tcaggcgccg taccggagaa tgccagaacc tgcctgatcg tatggccctg catgggcggc
     4861 gcagtgcaga tgtatcgcgt accggtggaa aaatttacag atcagggatg cgcctgtatt
     4921 ctttataatc cgcgcggcca cggtcgttcc gagggggaac tggaaattca aacggctttg
     4981 aatgacctag atgagattct tcaggagaat gttccttccg caattccgct ggtgatcgtc
     5041 ggccacagtg ccggagcgaa cgccggtctg caattcggag tccgctgtct tccgccgaag
     5101 caattcgtgc tgattgctcc ggttttggat tcacgtgaat ccctgtttta catgtatcaa
     5161 agaaaaacca ttgatgaatt cgtcaatatt ctttgcgcct atgtacagga tgacgctctg
     5221 gtaaggaaag tgcttgtcga tgatcaatgg ctggatgccg atctatggaa agcccgtcat
     5281 tacagacatc tgttggatcg tgttcatgcg cgtatgcgca taggcacatt cctggaaaga
     5341 ctctttattc ccggacacaa tgcctttgct caactggaaa ggttttccgc gcagacgcgc
     5401 atctttatgg ccggggagga cacctggtat ccggccgcga cgacacgtcg tttagcggac
     5461 aagtacggga tccctgttga aacactgccg gaagcgcaaa accatttttt taccggtgcc
     5521 tgggacagta tgtggaaaaa aattcttgat ctaatccttt tacccggcta gttttctttc
     5581 ttttcagcgt tgtttccctg ataaaaatca taaagggctt caacaagccg gcgtcctgca
     5641 aacgatttta agcgcaagcg gtcacttgat tcgtcaacgt ttgtggtcat ttctatgaat
     5701 ttcgattcat tcaggaaaca gtctgcgcga aggattactc ttgtagtttt ttgataaatt
     5761 tgtcatataa ccctcaactt agggagggta tatttattga tttatttcca caacatcagt
     5821 ctttcgtttc tggataaaaa actgttcgac aagatgaact ggaccatcca ccccaaaagc
     5881 cgcattggac tggtcggtga aaacggaacc ggtaaaacaa cactgtttag aatgatcatg
     5941 gatgacgtgc gccctgacga cggcacgctg gatattcccg gaaaaaaaca gaagatattc
     6001 ggctatctgc cgcaggacct ggtggaactg gaatcgattc ctctgattga ttatctcaag
     6061 caaaaaagcg gcatggccga cctggaaacg tccatcaaag atctggagca gaagttatcc
     6121 aaaatcaagc atgacacagc cgaatatcgc gagctttcgc aaaaatattc cgatgcgaca
     6181 gcattattcc atgccaggga cggttacgct tttgaatcca gagccaagca gatgctcaag
     6241 gggttcggct ttaaagaaag tgattttaaa caaaattgcg attcgttttc cggtggctgg
     6301 aagatgcgca ttttgttgac atcgattctt ctggcaaaac cggatatcat gctgttggac
     6361 gaaccgacga accatctgga tacggaaagc atggagtggt tggaatcgta tctcaaggat
     6421 ttccggggga ccatgattgt catttctcac gatcatttct ttttggataa agtcacgacg
     6481 cagatcgcgg agttgtcctc ctgcgctatt cacctttaca aggggaatta cacgcactat
     6541 ctgacggaaa aagaaaagcg gcaggacgct ttgaaaaagg aaatggaact gcaaaaatcg
     6601 caaatcaaaa aaatagaaga gtttgtggag cggttccgct acaaggcgtc aaaagcggcc
     6661 caggtgcaaa gccggattaa aatgctggaa aagttttcgg aggtaacccc ggagggaaaa
     6721 acaaagaatg tggccatgaa attccctgaa gggaagaaaa gcgccaaaga agtcgtcacg
     6781 gtaaccggcc tgggacaccg ctacggcgat ttgacggtgt ttggcgattt gaactttgcg
     6841 ctattccgcg gcgataaagt ggcctttgtc ggcatgaacg gctcaggaaa atcaacctta
     6901 tccagattac ttggcaaaat ggagaaccct tccagcggcg tggttaaata cggcgacggt
     6961 gtgaacatgg ctttttattc tcaggaaagt tcccagaacc tgaattacga aaaaaccatt
     7021 ctggaagaaa tcaatgccgt tccgaccgat gcgtcggacc aggaaaaacg caatctgctg
     7081 ggcacttttt tattttccgg agatgatatc ttcaaaccgg tatccgtgct ttccggcggg
     7141 gaaaagtcaa gacttgccct tctaaaaatc atgttgttgg ataccaattt cctgattctt
     7201 gacgaaccga ccaaccatct ggatatcaaa acaaaggaca tttttcaaaa cgctctgatc
     7261 cattaccgcg ggaccgtggc gatcgtgtcg catgaccggt atttcctgga tcatctggtc
     7321 aataaggtgt ttgaactgaa ggacggagca atccattact acaacggcaa ttattcttat
     7381 tttatcgagc agcgcagccg gccggcggat gccgcaggcg atgagaagcc tgcgcaaggc
     7441 gtgatcagaa atcagatcgt aaacgccaaa gagaaaaaac gtctggaagc ggaagaaaga
     7501 aacaaaacgt ataagatcag agcggcgctc aaaaaggaac tgtccgatgt ggagaaaaaa
     7561 atatccgg
//
LOCUS       DAOSME010000084         7506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_10938_length_7506_cov_51.557375, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000084 DAOSME010000000
VERSION     DAOSME010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10938_length_7506_cov_51.557375"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1871
                     /locus_tag="PLG98_10020"
     CDS             117..1871
                     /locus_tag="PLG98_10020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE33639.1"
                     /translation="MQDPIQTLKESIAAWSYVLDAAHNGIIIIDRHGVIVGCNEAARR
                     IFNDGSVFLSAGGLFSDLSKTGWQDVKNILETGQPQIGKRINLHQGAIIVNRTPIVIN
                     GQIEGVVSIFQDISEYENIITELKSYQELHRRLEAIIESSYDGLYITDGKAKTLLVNS
                     AYERITGISRNNLIGRNMKDLVKEKLYDHSVTLEVLKSKRSVTIMQHIKGGKEVIVTG
                     APIFDADGEISMVVTNVRDITELNQLRTQLEETRRLSSRYHQSILEQEELEHFLGEMV
                     VKSTSMLQVVRKAIKVAKVDTSVMLTGESGVGKSMLARFIHDMSARRENPFVTINCGA
                     IPESLIESELFGYEKGAFTGALPGGKAGLVEIGNKGTVFLDEIGELSANLQVKLLNVL
                     ENKIFTHVGGTKPVEVDIRIIAATNKNLDELVSKGLFREDLYYRVNVLPIHIPPLRER
                     RDDIPALVTKIMSKINQKHKTNKRLDPSVLESLRKFNYPGNVRELINMVERMFTMSED
                     EYICLMDIPVPDDLREPFMNQEAEDGTLPLKNALAGWEAQLIKEAIRRHKTIAESAKS
                     LRLHPTTLWRKMLKYSIK"
     gene            2252..3520
                     /locus_tag="PLG98_10025"
     CDS             2252..3520
                     /locus_tag="PLG98_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE33640.1"
                     /translation="MKQRLGIMILAVVLFMGISSFCLPVQTAAAEAPEVIKVGIIAPL
                     SGPAAWAGAAIKEGADLLVEEANNAGGIYIKDYKKKLKMEVIYGDSQSKPAIGVTVAE
                     KLITKDKVHFLVGEAFHSSVTMAVMELAPKYNIPIQSWMPTSNEISDKIKNNPKKYRN
                     FWKGDFASSAYGKTIHDTYIYLEQKGLYKPKNKTIAFVIEETDFGRSNADSAFELMQK
                     VGWKKVAYEVVPIGHNDFYPQLTKIKSLHPDVVVSCFTALNSGTSFLKQYQETGMTSS
                     HMAIYYPLYPELIKNAKTAADYLIWTPLLLNPAKIEYQAKFQERFKKRYGKDINNQNG
                     AGYDGMLGVFVAFQQASSLDPKKVVDAIAKTDYRAVLGRYQYNLDRHELKDGLDYIPI
                     PTAQILNGKNIIVWPTKMADTEYQPQPWIR"
     gene            3545..4282
                     /locus_tag="PLG98_10030"
     CDS             3545..4282
                     /locus_tag="PLG98_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE33641.1"
                     /translation="MMTTQPLLKITGVTKTFGGLVALDKVDMHVHQGEIVGLIGPNGA
                     GKTTLFNVIAGSFLPEQGEICFQGQNVTHLKAHNVCKLGIARTFQVPKPFKNLTLLEN
                     MMVGTCFGAGLGKSKDSLNKIGEILRFAGLEDKISSKADTLNLVERKKLEVSKALSTS
                     PRLVLFDEVMAGLNPSETVEMITFIGKLRDRGLTILLIEHLMKVIMTLSDRVVVLDYG
                     RKICEGNPQEVANNAQVIEAYLGKSNA"
     gene            4275..4979
                     /locus_tag="PLG98_10035"
     CDS             4275..4979
                     /locus_tag="PLG98_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE33642.1"
                     /translation="MLKINNLQVYYGDAHALWDINLQVRRGEICTLVGANGGGKTTLL
                     KAVCGLLPIREGSVLFDDSDLLAFKAEERVSLGLSMCPEGRKIFPGMTVEENLAIGAY
                     TKRAWKKRQSMMKQFYDLFPRLKERRKQPGGTLSGGERQMLAIARALMSEPKMLILDE
                     PSLGLAPNTVESVMEAVQKINGNGVTILLVEQNVNVSLQISQRGYVIESGRIVMSDRS
                     EMLLSNPHIKQAYLGL"
     gene            5002..5880
                     /locus_tag="PLG98_10040"
     CDS             5002..5880
                     /locus_tag="PLG98_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE33643.1"
                     /translation="MTSFPILFEQIVNGLVLGSMYALVASGLTLIWGTMKMLNFAHGE
                     LYMLGGFGLFFGVSYFHLAPWMAVPIVLLVIFILAALLEKGLISPMLGRPDWDVGAIV
                     VTLGIGIFVQNFAFKFWGGDFQEVPYMISGTTSFLNITLAWHRIYILSLAVIVLVAFW
                     QLMKRTRFGLALRATAQDIEAATLMGISYRRVYMATFGISASLAALAGTALAPIFSVN
                     PWMGSGPLIKAFIVVILGGLGSFAGAILGGFIIGISESLSVLFLGSGWKDLICFALLI
                     AVLMIRPSGLFGGKEW"
     gene            5877..6905
                     /locus_tag="PLG98_10045"
     CDS             5877..6905
                     /locus_tag="PLG98_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE33644.1"
                     /translation="MKNETYQDRLLKTFRAANPCLMWAFFVVLAILPLVVKSEYYLSV
                     MIICLIYATLAVSWNLMVGYAGILSFGHQAFYGLGAYFSAIMTMELHLSPWIGIPLGG
                     CFAALLSFVIGFPCLRLRIAPYIAIATLAFSEILLVICKNWVSLTRGEMGLSGIPPLR
                     DFSLFGLFPVVFSGGAKVPYYYVMLSIFFITLLFIYFQTSSRVGLAFKAMREEQDAAA
                     SLGVNITRYKLIAFLTGSFFAGVTGAFYAHYVSILAPDAVFGMGEMVEIVAITLIGGI
                     GTFFGPILGAFVLVIGLEALRGLADYRLMIYGAVMVILIIVMPNGLLAGFNHLLEKVR
                     NFGIFRKI"
     gene            6950..>7506
                     /locus_tag="PLG98_10050"
     CDS             6950..>7506
                     /locus_tag="PLG98_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE33645.1"
                     /translation="MPTVKINGINLYYEVSGQGHPLILIMGLRRNISWWYQQIPELSR
                     HFRVVAFDNRGAGRSDQPVMEYSIGLFADDTAGLMNALDIKNAHVLGISMGGYIAQEL
                     AIRHPEMVRSLILGCTSPGGNRAVMMSEERMKKFTANEGLTPEEILRKDMDIYFSDDF
                     IARQTDKMKEFIEISLRYYQPPDAF"
BASE COUNT         1921 a         1787 c         1910 g         1888 t
ORIGIN      
        1 agttgacaac attttacaag aatgccagac tgcttaccat gaatggtcat gtggcgcact
       61 tttcaatgag tcattttgac agcaattacc ctgacgcgta aacacgggtc gctatcatgc
      121 aagatcctat tcaaaccctg aaagaatcca tcgccgcatg gtcttacgtt ctggatgccg
      181 cgcataacgg cattatcatt atagatcgcc atggcgtcat tgtggggtgc aatgaagcgg
      241 cgcggcgaat tttcaatgac ggctccgtct ttctttccgc aggaggtctg ttttccgacc
      301 tcagcaaaac gggttggcag gatgtgaaga atatccttga gacgggacag cctcaaattg
      361 gcaaaaggat taacctgcat cagggcgcca tcatcgtgaa tcgaacccct attgtcatca
      421 acggccaaat tgaaggcgtg gtgtctattt tccaggatat ctccgaatat gaaaatatca
      481 tcacggagct caaaagttat caggaactcc acaggcgcct ggaggccatt atcgaatcct
      541 cttacgacgg gctgtatatc acggacggaa aagcgaaaac tcttttagtc aacagcgcct
      601 atgagagaat tacgggcatt tcccggaata atctcatcgg ccgcaacatg aaagatcttg
      661 taaaagaaaa gctgtacgat cactcggtta cgcttgaagt tctaaaaagc aagcgctccg
      721 tcaccatcat gcagcacatt aaaggcggga aagaggtcat tgttaccggc gcgcctattt
      781 tcgacgcaga cggtgaaatt tccatggtag tgaccaatgt gcgcgatatt acggaattga
      841 atcagctgcg cacacagttg gaggaaacgc gccgcctgag ttcgcgctat catcaatcca
      901 ttctggaaca ggaagagctg gaacattttc tcggggaaat ggtcgtcaaa agcacgtcca
      961 tgctgcaggt tgtaagaaag gcgatcaagg tcgcaaaagt tgatacatcg gtgatgctca
     1021 ccggcgaatc cggtgtgggc aagagcatgc tggctcgctt tattcatgac atgagcgcac
     1081 gcagggagaa tccctttgta accattaatt gcggcgcaat tcctgaatct ctcattgaaa
     1141 gcgaactttt cggctatgaa aaaggcgctt tcacgggcgc cttgcccggc ggcaaggccg
     1201 gcctggtcga aataggcaat aaaggaacgg tttttctgga cgaaatcggt gagctcagcg
     1261 caaacttgca ggtgaaactc cttaacgtat tggagaacaa gatcttcacc cacgtcgggg
     1321 gtacgaaacc tgtggaagtg gatattcgta tcattgccgc caccaacaaa aacctggacg
     1381 aacttgtttc caaaggcctt ttccgggaag acctttacta ccgtgttaac gtattgccga
     1441 ttcacatccc ccctttgagg gagaggcgcg acgatattcc ggcgctggtc accaagatca
     1501 tgtccaaaat caatcagaag cacaaaacga acaaacgtct cgatccatct gttttagaaa
     1561 gcttaagaaa gttcaattat cctggcaatg tcagagaact catcaatatg gtggagcgaa
     1621 tgtttaccat gtcggaagac gagtatattt gcctgatgga tattccggtt ccggatgatt
     1681 taagagagcc ctttatgaac caagaggcag aagatgggac cctgccgttg aaaaacgctc
     1741 tggccggatg ggaagctcag ttgatcaagg aagcgattcg tcgacataaa actattgcag
     1801 aatccgcgaa atctcttcgg ttacatccga caacgctctg gcgaaaaatg ctaaaatact
     1861 caataaaata agaatcgtta atattcttaa taggttatac gatcaatcct tgatattgca
     1921 aaaaagaaat agcattgcat aattgcattg attttaaata tatgtaatta ttactaattt
     1981 atattgatag aattttttca tgaatatatt ttgcattttt tcaatatacg gctgaatttg
     2041 ccttgatcca tctttcttaa atattcctaa taaattcttg caaataggaa ttattttacg
     2101 gctgccaata aatggtatgc ggtttgctca ctactgaatc cgtcaaaaaa cgtaaaggct
     2161 tgattggtaa gttgatcacc aggtaaaaac agctcgtcac cttaccgcga agagaacgaa
     2221 cgtgaaaact caaacaaaga aggaggcaaa gatgaagcaa cggttaggta tcatgatttt
     2281 agcggtcgtt ttgtttatgg gaatttccag tttctgcttg ccggttcaaa ctgcggcagc
     2341 tgaagctccg gaggttatca aagtaggcat tattgcgccg ttaagcggac ccgccgcctg
     2401 ggccggcgcc gccattaaag aaggagcgga tctcctggtg gaagaagcca ataatgccgg
     2461 cggcatttat atcaaagact acaaaaagaa actgaaaatg gaagtcatct acggagacag
     2521 ccagtccaaa ccggccatcg gcgttacggt cgcggaaaag ctcatcacca aagacaaagt
     2581 ccatttcctg gtgggcgagg catttcacag ctctgtgacc atggccgtca tggagttggc
     2641 gccaaaatac aatatcccga ttcagagttg gatgccgacc agcaatgaaa tttccgacaa
     2701 gatcaaaaac aatcccaaaa aataccggaa cttctggaag ggtgattttg ccagcagcgc
     2761 atacggtaaa accattcatg acacttacat ctacctggag caaaaaggcc tctacaaacc
     2821 caaaaacaag actatcgctt ttgtaattga agaaaccgac ttcggcagat ccaacgcgga
     2881 ttcggctttt gaactgatgc agaaggtcgg ctggaaaaaa gtggcgtacg aagttgtccc
     2941 gatcggccac aatgacttct acccgcagct gaccaagatc aagtctctcc atcccgatgt
     3001 cgtggtttcc tgcttcacgg cgttgaactc gggcacctct ttcctcaaac agtaccagga
     3061 gaccggaatg accagttcgc acatggccat ttattatccc ctctatccgg agctgatcaa
     3121 gaatgccaaa acagcggcgg attacctgat ctggacgccg cttttgttga atccggccaa
     3181 gattgagtac caggcgaaat tccaggaaag attcaagaaa agatacggca aagacatcaa
     3241 caatcaaaac ggcgccgggt acgacggcat gctgggtgtc tttgtggctt ttcagcaagc
     3301 aagctctctg gatcccaaaa aagtcgtcga tgccatcgca aaaacggatt accgcgccgt
     3361 tttgggacgt tatcaataca acctggacag gcatgagtta aaggatggtt tggattatat
     3421 tcctatcccg acagcgcaga tcttgaacgg gaagaatatt atcgtctggc cgacaaaaat
     3481 ggcagacaca gaatatcaac ctcagccttg gattcgatga tattccaacg caatcggaaa
     3541 gcgtatgatg acgacacaac ccttgttaaa gataacgggt gttacgaaaa catttggcgg
     3601 tttggtggcc ttggataagg tggacatgca tgtccatcaa ggcgaaatcg tcggtctaat
     3661 cggccccaac ggggccggta agaccacatt attcaatgtc atcgcggggt ctttccttcc
     3721 ggaacagggg gaaatatgct tccaggggca aaacgttacg cacctgaaag ctcacaacgt
     3781 ctgcaagctc ggcattgcca gaacatttca ggtgccgaag ccctttaaaa acctgacgct
     3841 tctggaaaat atgatggtcg gaacctgctt tggcgcaggc ctgggaaaga gcaaagactc
     3901 cttgaacaag atcggggaaa tcctgcggtt tgccggattg gaagataaaa tttcctcgaa
     3961 ggccgacacc ctcaatcttg tggaacgaaa aaaactggaa gtttccaaag ccttgtccac
     4021 gtcgcccaga ctggtgcttt tcgatgaagt catggcgggt cttaatccat ccgaaaccgt
     4081 tgaaatgatc acttttatcg gcaaacttcg cgaccgtggg ctgaccattc tgctgatcga
     4141 acatttgatg aaagtgatca tgacgctttc cgaccgggta gtggttttgg attatggccg
     4201 gaagatctgc gaagggaacc cccaagaggt tgccaacaat gcacaggtga ttgaggcgta
     4261 tctgggaaaa tcaaatgctt aaaataaaca accttcaagt atattatggc gatgcgcatg
     4321 ctttgtggga catcaacctc caggttcgcc ggggcgagat ttgcaccctg gtcggcgcaa
     4381 acggcggcgg taaaacgact ctgctgaaag ccgtctgcgg cctcctgccg atccgggaag
     4441 gatccgttct ctttgatgat tccgatttgc tggcgttcaa ggcggaagag cgggtcagcc
     4501 tcggactctc catgtgcccg gaaggaagaa aaattttccc ggggatgacg gtggaagaaa
     4561 acctggccat cggcgcctat acaaaacgag cgtggaagaa aagacaatcc atgatgaaac
     4621 agttctatga tcttttcccc cggctgaagg agcgccgcaa acaaccaggt ggaacgctca
     4681 gcggcggcga acgccagatg ctggcgattg ccagagcgct catgtccgag cccaaaatgt
     4741 tgatccttga cgagccctca ctgggtttgg cgccgaatac ggtggaaagc gtcatggagg
     4801 ccgtgcaaaa gatcaacggc aacggggtta cgatcctcct ggttgaacag aatgtgaacg
     4861 tgtctcttca gatttctcag cgagggtatg tcattgaaag cggcagaatc gtcatgagtg
     4921 accgttcgga aatgctcttg tcaaaccctc atatcaaaca ggcctatctg gggctgtgaa
     4981 aatgcacgtt caggagttta tatgaccagc tttcccattc ttttcgaaca aatcgtcaac
     5041 ggccttgttt tgggttccat gtatgcgctg gtggcaagcg gcctgacgct catctgggga
     5101 acgatgaaaa tgctcaattt tgcccatggt gagctttaca tgctgggcgg ctttggcctg
     5161 ttctttggag tctcttattt tcatttggcg ccctggatgg cggttccgat cgtcttgctg
     5221 gtgattttca ttctggcggc gctgctggag aaagggctta tctcaccgat gctgggacga
     5281 ccggattggg atgtcggagc gattgtcgta acactgggca tcggaatttt tgttcaaaat
     5341 tttgcattca aattctgggg cggtgatttt caggaagtgc cctacatgat ttcaggaacc
     5401 accagctttt taaacattac cctggcctgg caccggatct atatcctgag cctggcggtg
     5461 attgtgctgg ttgctttctg gcagttgatg aaaaggaccc gattcggcct tgcgctgcgc
     5521 gccacggccc aggatattga agccgcaacc ctcatgggta tttcctatcg tagagtatat
     5581 atggcaacct tcggcatcag cgcatctctt gcggcccttg ccggcacggc cctggcgcct
     5641 atcttttccg tcaacccctg gatgggaagc ggccctctga tcaaagcgtt tatcgtggtc
     5701 attttaggcg gcctgggcag ctttgcgggc gcaattctgg gcggattcat catcggcata
     5761 tcggagagct tatccgtttt gtttctggga tcgggatgga aggatctgat ttgctttgct
     5821 ctcctcattg ctgttttgat gatccggcca tccggacttt ttggaggtaa ggagtggtga
     5881 aaaacgaaac ttatcaggac cgcctgctca aaacgttcag ggccgccaat ccctgcctca
     5941 tgtgggcttt tttcgtcgtc ctcgcgatat tgccccttgt tgttaaaagc gaatattatc
     6001 tgagcgtgat gattatctgc ctgatctatg caacgctcgc ggtcagctgg aatttaatgg
     6061 tgggctacgc cggcatcctc agttttggtc atcaggcctt ctacggactc ggcgcctatt
     6121 tctccgccat catgaccatg gaacttcatc tgtctccgtg gatcgggatc cctttgggcg
     6181 gttgcttcgc ggccctgctc agttttgtca tcggctttcc ctgtctgcgt cttcgcatcg
     6241 caccctatat cgccattgcc acgctcgctt tttctgaaat tctgctggtg atttgcaaaa
     6301 actgggtatc cctgacgcgc ggggaaatgg gtctttccgg gattccaccc ctccgggact
     6361 tttctctttt cggcctgttt cccgttgtct tttcgggcgg cgcgaaagtg ccttattatt
     6421 acgtcatgtt gagtatcttt tttataacac tgctctttat ctattttcaa acaagctcca
     6481 gggttggcct ggcctttaag gccatgcgcg aagagcagga tgccgccgcc agcctggggg
     6541 taaacatcac gcggtataaa ctgatcgctt ttttaacggg ttcctttttt gccggagtga
     6601 cgggcgcttt ttacgcgcat tacgtgtcca ttctggcgcc ggatgccgtt ttcggaatgg
     6661 gggaaatggt ggaaatcgtc gcgatcacgc tgatcggggg aatcggcacg tttttcggtc
     6721 cgattctcgg cgcctttgtt ctggtcatcg gactggaagc ccttcgggga ttggcggact
     6781 acagactcat gatctacggc gccgtcatgg tgatcctgat tattgtaatg cccaacggtc
     6841 ttctggccgg gttcaaccat ttattggaaa aggtgcgaaa ctttggaata tttcgcaaaa
     6901 tctaaaatta acgaaattaa cgccttgaac acatcaattg aggatggata tgccgaccgt
     6961 caagattaat gggatcaatt tgtattatga ggtgtccggc cagggtcatc ccctgattct
     7021 gatcatggga ctccgccgca atatctcctg gtggtatcag caaattccgg agctgtcccg
     7081 gcatttcaga gtggtcgctt tcgacaaccg gggtgcggga cgttcggatc agccggtgat
     7141 ggaatattcc atcggtctgt ttgcggatga tacggcaggc ttgatgaacg cgctggatat
     7201 aaagaatgcc catgtcctgg gtatctccat ggggggatat atcgctcagg aactcgccat
     7261 ccgacatccg gagatggtgc gcagcctgat cctgggttgt acgagccccg gagggaaccg
     7321 ggcggtcatg atgagcgaag agcgcatgaa aaaatttacg gccaacgagg gactgacgcc
     7381 cgaggaaatt ctccgcaaag acatggatat ttatttttcc gacgacttca tcgccaggca
     7441 gacggacaaa atgaaggaat tcatcgaaat ctcccttcgg tattatcagc cgcccgacgc
     7501 tttttt
//
LOCUS       DAOSME010000085         7493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_10954_length_7493_cov_18.294300, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000085 DAOSME010000000
VERSION     DAOSME010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10954_length_7493_cov_18.294300"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..2123
                     /locus_tag="PLG98_10055"
     CDS             168..2123
                     /locus_tag="PLG98_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOE33646.1"
                     /translation="MSKAYMGKILMVDLSSKTMTEETIPDSVYQQYLSGMGLGAYILY
                     NRIPAGADPLGPDNILGFCSGLLTGTGSLFSGRWTLVGKSPLTGGWGDANCGGTFSPA
                     IKHCGYDAIFFKGISPNPVYLYVKNGKTFLKDASDLWGMDAVETEEKLKKQYGERAGV
                     AVIGPAGEKVSLISGVCTDKGRIAARSGLGAVMGVKKLKAVVLDGVQRIQAQDPKSMK
                     SLSQKCIKWTNFPMPLGLLPGVGLALMGTFMGATPVMFAQDGLLYKTILRTWGTGGMN
                     QMSIEMGDSPLKNWEGSSRDFHLGKSKTVNPDIIKKAEIVKYHCYSCPLGCGGICATK
                     GKYSHTHKPEYESILALGGLCMNEDLDSLFYLNELLNRAGMDTISAGATVAFAIECYE
                     KGILTKEDTDGLELKWGNTEAIVALIRKMVDREGIGDILADGSKVAAAKIGRGAEECT
                     VHAGGQEPAMHDGRGDPGFNVHYSVEPTPGRHTIGAHLYYEMFQLWKRVKGLPKPNLL
                     YMKGSKYKINKEKAEASAACSKFMSMGNGAGMCMFGLFIGAKRIPTFDWLNAATGWTF
                     TPEEYMTIGERIQTIKQNFNIRHGIEPRKNFISSRAQGKIPQKKGPNKGRFVDVEKLA
                     ADYWEQFGWDRTNGKPKEETLKKLGIK"
     gene            2135..2608
                     /locus_tag="PLG98_10060"
     CDS             2135..2608
                     /locus_tag="PLG98_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE33647.1"
                     /translation="MSYTITEKCNGCGACARVCPADAITGEKKKIHSINAALCIECGA
                     CGRVCPQGGVLDADGVLCVMIKRSEWTKPEIIVEKCIACGICVGTCPAGCLAMSDRPR
                     NQGVDAFPYLKDAKSCIGCGFCALECPVDAIVMQKPEKAKKESAEMKAAGSAASA"
     assembly_gap    2877..2976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2977..4361)
                     /locus_tag="PLG98_10065"
     CDS             complement(<2977..4361)
                     /locus_tag="PLG98_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOE33648.1"
                     /translation="MNKKKKIIISCLILLLIGALFYFMNIYSTVAADIKLRGSSSPPL
                     TEKKQIAKPASSDHSSYKGQIPDSIVAFSSGYAVVVDKEHQKLYVFYKSGKYSKVFET
                     VCSTGKNPGSKRIAGDAKTPNGIFFATRILENPGPSETYGSLAFTLDYPTLSDKRAGK
                     DGNNIWIHGTTKPLLPQQSNGCVVLSDEDMKKLANFIHLNRTPVIISESINWVDQDYL
                     SDSREEFEKILWSWNKAFVEKNLKEIDSLYAEGAQIRGKKREDLHYKIKNLDFIKKHF
                     VLQPRDIAILQEGDNAVIIFDQIFDVKDNNTFQGFYNKLVLEKINENWYVVDESSISE
                     PADRRLATAKDTQKDAVSPEKIAEKNIRSLVRKWRASWESGSMKTYRACYASDFQSRD
                     MGLDAWIAHKIKVRQKSSNIKISVNNLQISMSGNTARATFVQQYSSSILRTKGSKTLE
                     LKKSADGWKIH"
     gene            4593..6428
                     /locus_tag="PLG98_10070"
     CDS             4593..6428
                     /locus_tag="PLG98_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33649.1"
                     /translation="MSNTSKDINSTEKLLNVIRGKDEASFGGLGKQKISLTPDKKTGI
                     FQSGFLEKFLSKKKYTVGIDIGREFICLVKTAQDSNHQPILVDKKIITIDPRFVIGSP
                     EFNQLVKTSVLNFCGNVADCDIWTKISTSQVGVYFFTIPRVPKKQIQKVIFWTAKKEG
                     FYDEEKQIFDFEIHGELVEQGTPKYSVMFYTAQKAEVARIKSFADNIGINLKGITIVP
                     FAMQNIFRSKWIPASEEIFASLFIGDNYSRIDVYDKENLVMTRGIKTGSLSSMAEAVV
                     AGFSGRKGDLKLDQTEAKKIVCTMRPESGGAKEADDRQKFAREDILNMIAPVWERLAR
                     QIDLTLKTSPIGNKKVEKMYVLSPINFDQSLLDYMGNQLDTRIEIFDPFKNRKSVISE
                     QSLSSSERILISPALGFALSDNSRTPNAVFTYAEKRQDIKSRRINKVIFMAFLAALMV
                     CIGTLFYQGTKFSTLKKNNLALEKELALFSPMVSQETILQEVNRIKLQRDAVRQYAQK
                     YLGVAVIGEISDTTPENVRLINLRLRQAAGSPAAAGQKTEHNGLVLEGIIFNKKDMLE
                     SDLTQYILKLENSPLFNTVSVQNKNIVNFDKKDVIHFVLSAKLGS"
     gene            6428..6967
                     /locus_tag="PLG98_10075"
     CDS             6428..6967
                     /locus_tag="PLG98_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33650.1"
                     /translation="MMKNFNLNAQQKKSILYAVICSGIVLIIVLVGILPYSFKVSNQQ
                     NENEKLTSQIKEQRELGPVYKTLTGNVEQTDVLILPHAQKTALPRQDITIIQKDFQAA
                     AQKSGLKIISYAPEINTSASPSASFLHNLELKGELAGFRKLLIALGALPYVERIEEIS
                     IQQNAGAMDFKMKIWTAVQ"
     gene            6984..7484
                     /locus_tag="PLG98_10080"
     CDS             6984..7484
                     /locus_tag="PLG98_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33651.1"
                     /translation="MAKLKKREIVILAIAALFVLYAVYVYLIADRQKGKKVKPDQETV
                     KVEAVVSGITGELNKSKISDFDHYVIQRTQTDWDKNPFLKRELYRAWLAKDSKGKDGV
                     AAVPIIYSGYIETGKNRLAVLNGIEYRVGEALQEEGFVLKKIMPSKVVIFDKRIGSNL
                     EIPLQE"
BASE COUNT         2116 a         1544 c         1814 g         1919 t
ORIGIN      
        1 ttcttcctga aattgccaaa gcagccctgc ttgacggagc aaccatttat aatccggaag
       61 attcagacta tgaagataat ttattagtgt tggaagcggc atgggaaggc aagccccttg
      121 accggaataa aattaaaaaa gggtaaacat tacaagagag agtttttatg agtaaagctt
      181 atatgggaaa aatcctcatg gtggatctgt ccagcaaaac catgacggaa gagacaatac
      241 cggatagtgt gtaccagcaa tatctgtccg gcatggggct cggagcctac atcctttaca
      301 accgaatacc ggccggcgcc gaccctctgg ggccggacaa tattctgggc ttctgttccg
      361 gtctgctgac cggcacaggc agtttatttt caggacgatg gacgctggtc ggcaagtcgc
      421 ctctgaccgg aggctggggc gacgcgaatt gcggcggcac gttttctccg gccatcaagc
      481 attgcggcta tgacgccatt ttcttcaaag gcatcagccc caatccggtt tatttatatg
      541 taaaaaacgg caagacgttt ctcaaagatg cctcggacct gtggggaatg gatgccgtcg
      601 aaacggagga gaaactcaag aaacagtacg gcgagagagc gggcgtggcg gtcatcggcc
      661 ctgccggaga aaaggtttct ttgatttccg gtgtttgtac cgacaaaggc agaatcgccg
      721 cccgatcggg attaggcgct gtcatggggg tgaaaaaact taaagcagtg gtccttgacg
      781 gtgtgcaacg gattcaagct caagacccta aatcaatgaa aagtctgagt caaaaatgta
      841 tcaagtggac caacttcccc atgccgctcg gcctgcttcc gggcgtgggc ctggctctta
      901 tgggaacatt tatgggggcg actccggtca tgtttgccca ggatggtctc ctgtacaaaa
      961 ccattttaag aacatggggc acgggaggca tgaatcaaat gtccattgag atgggcgatt
     1021 cacccttgaa aaactgggaa ggttccagtc gggatttcca cctgggtaaa tccaaaaccg
     1081 tcaatccgga tatcattaaa aaggcggaaa tcgtaaaata tcactgttat tcgtgtccgc
     1141 tgggatgcgg cggcatttgc gccaccaaag gaaagtattc ccatactcat aaacccgaat
     1201 atgaaagcat cctcgcgttg ggagggttgt gcatgaatga agatctggac agcctctttt
     1261 atttgaacga acttcttaac cgggcgggca tggacaccat ttccgcgggc gcaacggtgg
     1321 ccttcgccat tgagtgctac gaaaaaggta ttctgacgaa ggaagatacg gacggtttgg
     1381 aattgaaatg gggcaatacg gaagcgattg tcgccctcat cagaaaaatg gtcgaccggg
     1441 aaggcatagg cgatattctg gccgacggat ccaaagttgc cgcggcaaaa atcggccggg
     1501 gcgcggaaga gtgcactgtt catgcgggag gtcaggaacc ggccatgcat gacgggcgcg
     1561 gcgatcccgg cttcaatgtc cattacagtg tggaacccac gccgggtcgg catacgatcg
     1621 gcgcgcattt atattacgaa atgtttcagc tctggaagcg ggtcaaagga ttgcccaagc
     1681 ccaatcttct ctatatgaaa ggcagcaaat ataagatcaa taaggagaag gccgaagctt
     1741 ccgccgcatg cagtaaattt atgagcatgg ggaatggcgc gggcatgtgc atgttcggcc
     1801 tttttatcgg agcgaaaagg attcccacgt tcgactggct aaatgccgcg accggctgga
     1861 cgtttacccc tgaagaatac atgaccatcg gcgaacggat tcagacgatc aaacagaatt
     1921 ttaacatccg gcacggcatc gaaccgagaa aaaactttat cagcagccgc gcacagggga
     1981 agattcctca gaaaaaaggt cccaataagg gacggtttgt cgatgtggaa aaacttgccg
     2041 cagattactg ggaacagttt ggttgggaca ggaccaacgg caaacccaaa gaggaaacat
     2101 tgaagaaact gggaatcaaa taagaggtaa cagcatgtca tatacaatta cggaaaaatg
     2161 caatggctgc ggcgcctgcg ctcgagtgtg tcccgcggat gccattaccg gtgaaaaaaa
     2221 gaaaattcat tcgatcaacg cagcgctatg catcgaatgc ggcgcctgcg gcagggtttg
     2281 tcctcaggga ggcgtcttgg atgccgatgg cgttttgtgc gtcatgatca aacgatctga
     2341 atggaccaag ccggaaatca tcgtggaaaa atgtatcgcc tgcggcatct gtgtcgggac
     2401 ctgtcccgct ggatgcctgg ctatgtcgga ccggccgcga aatcaaggcg tggacgcttt
     2461 cccatatctg aaggacgcca aatcctgcat cggttgcgga ttctgcgctt tggaatgtcc
     2521 ggtagatgcc atcgtcatgc aaaagccgga aaaggcaaaa aaagaatctg ctgaaatgaa
     2581 agcggcgggt tcggcagcgt ccgcgtgagg cattacatta agaaagcaat accgtcatgc
     2641 ctgcgtaaac gtgatgtccc gaaggttaac acggtatcgc aggacaggag gatgacgaaa
     2701 tactgaaaat atgactgccg tcgtttttct aaccaaatta aaaaaccggt gctataaaac
     2761 accggttttt ttatggacaa cgctgctggt ttaagtggac aacaatctct acataatttc
     2821 cctatgtatt ttccatccgt cggcactttt tttcaattcc agagttttgg aaccttnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctat gtattttcca tccgtcggca
     3001 ctttttttca attccagagt tttggaacct ttggttctta aaatggaaga actgtactgt
     3061 tgcacaaatg ttgccctggc tgtattgccg ctcatggaga tttgtaaatt attgacgctg
     3121 attttaatgt tgctgctttt ttggcgaact ttaatcttat gcgctatcca ggcatcgagt
     3181 cccatgtcac gggattgaaa atcagacgca taacaggccc gatatgtttt catgctgccc
     3241 gattcccagc ttgcccgcca ttttctgacc aggctccgga tgtttttttc ggcaattttt
     3301 tcgggggaga cggcatcctt ttgcgtgtct ttggctgttg ccaggcgcct gtccgcaggc
     3361 tcggatatcg aggactcatc aacaacatac cagttttcgt ttattttttc taaaaccagt
     3421 ttattataga atccctgaaa agtattgttg tccttcacgt caaaaatctg atcaaaaata
     3481 atgacggcat tatcgccttc ctgcaggatg gcgatgtcgc gaggctgtaa aacaaaatgt
     3541 tttttaatga aatccaaatt tttaattttg tagtgaaggt cttctcgctt ctttcccctg
     3601 atctgggcgc cttcagcata caaagaatca atttccttta aatttttttc aacaaatgct
     3661 ttgttccacg accataatat cttctcgaat tcctctctgg agtcggaaag ataatcctga
     3721 tcaacccaat tgatggattc ggaaataatg acgggtgtcc tgtttaaatg gataaagttg
     3781 gccagtttct tcatatcttc atcactcagc acgacacaac cgttggattg ttgcggaagc
     3841 agtggctttg tggttccgtg aatccagata ttattgccgt ctttgcctgc tcgtttatcg
     3901 gacagagtcg gataatccag cgtaaaggcc agtgaaccgt acgtttccga aggtcccggg
     3961 ttttccagga ttctggtggc gaagaaaatt ccattggggg tttttgcatc tcccgcaatc
     4021 cttttggaac cggggttttt accggtggaa caaaccgttt cgaagacctt ggagtatttg
     4081 cctgatttat aaaagacata taatttttga tgttctttat cgacaacgac cgcgtatcct
     4141 gacgaaaagg caacaattga atccggtatc tgacctttgt aggaagaatg gtctgatgac
     4201 gccggtttcg cgatttgctt tttttccgtc aagggcggcg atgaagagcc tcttaattta
     4261 atatctgcag caacggtgga ataaatattc atgaagtaga acagagcgcc gatcagcaga
     4321 aggatgagac aagagattat tatttttttc ttcttattca taagaacagg taaattcaga
     4381 gtttttttaa aaaaagtatt gttcttttaa caaaaaactg ataatcattc aagaaattaa
     4441 tacaaaataa aaattgctct tctgtgaggc tttctttttt ttgtgagaac aaagaatcta
     4501 ataaaatgat ttacaggaat ttggaattac attataaatt taaaaaaatg atttattaga
     4561 ttggtgcatg cgtcaacgac caggagatta ttttgtctaa taccagcaaa gacataaact
     4621 ctacggaaaa acttctcaat gtgatacgcg ggaaagacga ggcgtctttc ggcgggctcg
     4681 gaaaacagaa aatttcttta acgccggata aaaaaaccgg tattttccaa tcaggatttc
     4741 ttgaaaaatt cctttccaaa aagaagtata cggtcggtat cgatatcggg cgtgaattta
     4801 tctgcctggt aaaaacagct caagacagca atcatcagcc gattttagtc gataaaaaaa
     4861 tcatcacaat agatccccgg ttcgtcatag gttctccgga attcaatcag ttggtcaaaa
     4921 cgtcggtttt aaatttttgc ggtaatgtcg ccgattgcga catctggacc aagatatcca
     4981 caagtcaggt cggcgtttat ttcttcacaa ttccccgtgt tcccaaaaag caaattcaaa
     5041 aagtcatttt ctggacggcc aaaaaagagg ggttttacga tgaagaaaaa cagatttttg
     5101 attttgaaat ccatggcgaa ctggtagagc aggggacgcc taaatattcg gtcatgttct
     5161 ataccgctca aaaagcggaa gttgcgagaa taaaatcgtt tgccgataat atcggcatta
     5221 atcttaaagg aattaccatc gttcctttcg ccatgcagaa tatttttcgg tcgaaatgga
     5281 ttcctgcctc ggaagaaatc tttgccagtc tgtttatcgg tgataattat tcgcgcatcg
     5341 atgtttatga caaagaaaat ctggtcatga ccagagggat aaaaacaggc agtctttcca
     5401 gtatggcaga ggcggttgtt gcgggcttta gcggaagaaa aggagatttg aagctggatc
     5461 agactgaagc caagaagatc gtttgcacca tgaggcccga atccggggga gcgaaagagg
     5521 ccgatgaccg gcagaaattt gccagggaag atattctgaa tatgattgcc cccgtttggg
     5581 agagattggc gcgtcagatt gatttaacac tgaaaacgtc gccgatcggc aataaaaaag
     5641 tcgaaaagat gtatgttttg tcgccgatta attttgacca gtctttgctt gattacatgg
     5701 gcaatcaatt ggatacccga attgaaatat ttgatccttt caagaataga aaatcggtta
     5761 tttccgaaca atcgttaagt tcttccgaaa gaatactgat ttctcccgct ctggggtttg
     5821 ccctgtcgga taacagccgc acgcccaatg ccgtatttac ctatgcagaa aaacgtcagg
     5881 acattaaatc acggcggatc aataaggtta ttttcatggc cttcctggcg gcgctgatgg
     5941 tttgtatcgg tacgttattt tatcagggga caaaattcag tacgctgaaa aagaataatc
     6001 tggccctgga aaaggaactg gcgcttttca gtccgatggt gtcgcaggaa acgattcttc
     6061 aggaagtgaa caggatcaaa ttacagagag acgcggtccg gcagtatgcg caaaaatatc
     6121 tgggggtcgc ggtgatcgga gagatttcgg atacgacccc ggaaaacgtg cgtctcatta
     6181 atttgcgcct gcggcaagcc gccggttctc ccgcagcggc tggtcaaaag acggaacata
     6241 acggtcttgt tctcgaaggc atcattttta ataaaaagga tatgctggaa tccgatttga
     6301 cacaatacat tttaaaactg gaaaattctc ctcttttcaa tacggtgtcg gtgcaaaata
     6361 aaaatattgt taactttgat aagaaagacg tcattcattt tgtattaagc gccaaactgg
     6421 gttcgtgatg atgaaaaatt ttaatctgaa tgcgcaacag aagaaaagta ttctctacgc
     6481 cgtgatctgc agcggaattg tgttaattat tgttttggtg ggcatactgc cttactcgtt
     6541 caaagtatcc aaccagcaga atgaaaatga aaaattaacg tcacaaatca aggagcaaag
     6601 agagctcggg cctgtctata agacattgac cggcaatgtg gaacagaccg atgttcttat
     6661 tctgccgcat gcgcagaaaa cggcgctgcc tcgccaggac attacaatca ttcagaagga
     6721 ttttcaggcg gcagcgcaga aatccggatt aaaaatcatt tcgtatgctc cggaaattaa
     6781 tacatcggcc agtccatccg catcgttttt acataacctg gaactgaaag gggaactggc
     6841 tggattccgc aaattgctga tcgcgctggg cgcccttcct tatgtggaaa gaatcgaaga
     6901 aatcagcatt cagcaaaacg ccggcgccat ggattttaaa atgaagattt ggacagccgt
     6961 tcaataggca ggtaaaggga tttatggcca aattgaagaa aagagaaatt gtcattttag
     7021 caatagccgc gctgtttgtc ttgtatgccg tctatgttta tctgattgct gatcgccaga
     7081 aaggcaaaaa agtgaagcct gatcaggaaa cggtgaaggt tgaagcggtc gtcagcggga
     7141 taaccggtga gttgaataaa agtaagattt ccgattttga tcattatgta atccagagaa
     7201 cgcagacgga ttgggataag aatcctttcc tgaaaagaga attatacaga gcctggctgg
     7261 ccaaagacag caaaggcaaa gacggggttg ccgccgttcc gatcatttat tcgggttata
     7321 tagagacggg gaaaaacaga ctcgccgtgc ttaacggcat cgaatataga gttggtgaag
     7381 cattgcagga ggaagggttt gtcttgaaaa aaatcatgcc ttccaaagtt gtaatatttg
     7441 ataaacgaat cggaagtaat ctggaaatcc cgctgcagga ataaaatttg tca
//
LOCUS       DAOSME010000086         7449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_11027_length_7449_cov_20.480525, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000086 DAOSME010000000
VERSION     DAOSME010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11027_length_7449_cov_20.480525"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLG98_10085"
     CDS             complement(<1..290)
                     /locus_tag="PLG98_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33652.1"
                     /translation="MPYIERSDDGKIIGLRTIAGPNSTEEKSINDQEVLDFLKDNLDN
                     NFLKNLLVHSDAGLIRLLEDLIALLTKKNIILFTELPEKAQAKIIEREKIRE"
     gene            522..2696
                     /locus_tag="PLG98_10090"
     CDS             522..2696
                     /locus_tag="PLG98_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOE33653.1"
                     /translation="MSNDHDDCNENTWNVLENQDVHDDPLLDCLIVLSRIYGRNVSKA
                     SLIAGLPLVQNRLTVKLFNRAANRADLSSRTIAKPLNDISSIHLPAILLLQNREACIL
                     VEKNSSLNRLKIIIPQSGSGEQEISFEELEKIYTGYGIFVRPKYQTDQKSVADEKADK
                     SKHWFWGTIMESWRVYRDVFLAAFLINVFGLVGIFYVLNVYDRVIPNNATETLWVLSI
                     GVLVIYLFAVVMRALRSYFVDEAAKKTNLKISAMLLQKVLNLKMESRPQSIGSFTKNL
                     QEFESIRDFITSFSITAVIDLPFTLLGLLVVWWIGSYIVLIHVIIIVLLIIYAYFIQA
                     PLQKVVEKTLKASAQKNAILVEGVAGLETIKMLGAEGQIQRAWEEAVSYISKWGNKSR
                     MISSSVQDVSYFLQNLMTVAVVVAGVYMITAGSLTSGGLVALVILSRQVIAPMAQVVN
                     LATRYHHVREALKMLDDIMNLPVERPAGKTFLPRKRFDGVIGIKNLSFSYPGQTTMVL
                     NNISLEIAAGEKVGIIGPVGSGKTTLGKLMLGLYEPVSGMVTMDGTDIRQIDPAELRH
                     FLGYVPQDITLFQGTLRDNITMGVNNVDDQNVLQAAEMAGIIEFAKIHPSGFDMKVEE
                     FGRGLSGGQRQCVAMARALLLDPPVLVLDEPTSNMDNRSEIRLKRYLAQTVKEKTMVI
                     ITHRASLLDMVTRLIVIDHGSIVADGPKDFVLEAMRKGQLNL"
     gene            2712..4154
                     /locus_tag="PLG98_10095"
     CDS             2712..4154
                     /locus_tag="PLG98_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HOE33654.1"
                     /translation="MKKLFDFFKNNPFVESFRDLPETDKISFKELKNNFKMNYLFRRL
                     PEEDPDFATDVRETILAQTPQGGRLIIWATLILLVIFIVWASFSELEEVTRGEGKVVP
                     SSHVQIVQNLEGGIISEILINVGDKVKKDQLLLKMDPTRFSSSFEENRVKYLSDKAKA
                     ARLKAEATGSALVIPEDVLKERPDIAERERQLFQTRRMELGSSSEIKRQQINQRRQEL
                     KELEAKLVELNRTYSLLQKEIGMIKPLVNKGAASDVEVLQLERQASQLEGEIDRVRHA
                     IPAAQAKLQESEVALRELSLSYRSKSSTESSEVLKELDESASSSLALKDRLDRTAVRS
                     PVDGIVNRVMVKTVGGVVQPGMDLVEIVPMHGNLLIEAKIKPSDIAFLRPGQKATIKF
                     TAYDYTVYGALDAELESVGADSITDDKGNSYFLVRLRTDKNYLGTAQHPLPIMPGMVT
                     TVDILTGKKTVLSYILKPVLKARYMALRER"
     gene            4200..4838
                     /locus_tag="PLG98_10100"
     CDS             4200..4838
                     /locus_tag="PLG98_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE33655.1"
                     /translation="MTILLSSPVPGVMIRWEAHLKGHCKIKKALSIEQLRSLCGNRQF
                     DVILLHRMLIDMSSFRTLRSAFPAARFFLLTDHPDEEEALTFLRLGIVGYANTYIAAP
                     RLLEALHVITEGGVWLGQKIIQRLITGVSNTLKPEDKKDQVSLLPANLTKSQRNIAER
                     VARGQSNLEIAADLNITERTVKAHLTSIYEKTKTGNRLNLALLINLGKNPSG"
     gene            complement(4931..>7449)
                     /locus_tag="PLG98_10105"
     CDS             complement(4931..>7449)
                     /locus_tag="PLG98_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE33656.1"
                     /translation="NTATGTITVDIVDDIPSAGDQSITVDAGTVVGTNLLITLDVSGS
                     MNDPSGVDGKTRLDLAKDSLEDLIQAYEDNGPVMVRLVTFSSSSTIVGDQWMTADDVI
                     AVINGLSAGGNTNYDAAINNAKAAFDDPGAIAGAQNISYFLSDGQPNAPYGSVGINAA
                     EQASWETFLANNDITSFALGMGTGVTTSNLAPIAYDGLNTVQIDPIVVTDLSQLDDVL
                     LDTVIVPSTITGNLISGSVPATFGADGPAVLKVVSIAHDANGDGIDEIYDTSSAGYNT
                     STTTLTLSTQAGGTLAVNFSTGDFTYTGPASVTLLASDVFTYKVMDADGDTASATLTV
                     NLPTDGILVVGTNADDISTQTVDHYIDDAAPYAGVITGSGGNDVLVGDLNATSIAGKT
                     LNLVLMLDSSGSMDAMIKFNGVDMTRLAALKLSVENTLNNLANGPAENVRVHIVDFDT
                     HARDINPNSGTYFDIKINGAKVAGTLTDAINAVNAIDNGPSGPEGGTNYEAALHTAYV
                     WANSGAPYSPGSNVVSQAIFVSDGEPTYYYRGDEYSQLGGDGVYFYQENIKQILGTGT
                     NDTVSEVAQLEAKGFTIEAVGINVTDMNLNHLNQVEGEAADSNPDVATNITTGEQLSS
                     VLTDLTSTVVSDAGNDNIVGGDGDDIIFGDTINYNYTGTTSQMMADIKANHLTLSAES
                     GLTGGNDIINAGAGNDIVYGQEGNDIIYGGEGNDILSGGSGNDILSGGTGSNTLTGGA
                     GADTFTLSAQANDTVKDYSQTDGDKVDISAILDVADESVAKTYLGFHNNGDGKAVLEV
                     YNSSTDHSAGNLVGSVTFDSITDATNLDSLLGKVDIDHDA"
BASE COUNT         1968 a         1835 c         1724 g         1922 t
ORIGIN      
        1 ttttcacgta ttttttcccg ttcaatgatc tttgcctggg ctttttccgg cagttccgta
       61 aaaagaataa tgttcttctt cgtcaacagg gcgatgagat cttccagcag cctgatcaat
      121 cctgcgtcag aatggactag aaggttctta agaaaattgt tgtccagatt gtctttaaga
      181 aaatcaagaa cctcttgatc gttaatcgac ttttcctcgg ttgaattcgg accggcaatg
      241 gttctcaatc caataatttt cccgtcgtca gaacgctcaa tataaggcat cttgattcat
      301 ccttaactca aaaaagttaa aacgataatg cattatttca cgcataccgg ataacccgat
      361 agcaattcct ccattctgat tgccgatata taccttcttt cattgatgat acgattttta
      421 aaaatatata tactttttta aaaaaaatgt ataataaaaa atactgatat atttcatgtt
      481 gggaaaaata taacaaaaac tttctattgt tgatgcgatt tatgtcaaac gatcatgacg
      541 actgcaatga aaatacctgg aatgttttgg aaaatcaaga cgttcacgac gacccgcttc
      601 tggattgcct gatcgttctg agcaggatat atggccgtaa tgtatcgaaa gcaagcctga
      661 ttgccggtct gcctctggtt cagaaccgtc tgacggtaaa gctttttaat cgtgccgcga
      721 atcgggctga tttatcttcc cgcaccattg ccaaacccct caacgacatc agctccattc
      781 atttgccggc catattgctg ctacagaacc gtgaggcatg cattctggtt gaaaaaaaca
      841 gttccctcaa ccgcctgaaa attattattc ctcaaagcgg cagcggagag caggagattt
      901 ccttcgagga actggaaaaa atctatacag gatatggcat cttcgtccgc cccaaatatc
      961 agaccgatca gaaatccgtt gccgatgaaa aagctgataa atcaaaacac tggttttggg
     1021 gaaccatcat ggaatcatgg cgtgtttacc gtgatgtttt tctggcggcc tttctcatca
     1081 atgtgttcgg tctggtcggg atcttttacg tcctgaacgt ctatgatcgg gtcattccca
     1141 acaatgccac agagacgctc tgggttttat cgatcggcgt gttggtcatt tatctttttg
     1201 cggtcgttat gcgagccctc agaagctatt tcgttgatga agcggccaaa aaaaccaatc
     1261 tgaaaatttc agcaatgctc cttcagaaag ttctcaacct gaagatggag tcgcgccctc
     1321 agtccatcgg ttccttcact aaaaatttgc aggaattcga gagtattcgt gatttcatca
     1381 catcgttttc gattaccgcc gtaatcgacc tgcccttcac gcttctgggc ctgctggtcg
     1441 tctggtggat cggcagctac attgttctca tccatgtgat catcatcgtc ctgctgatca
     1501 tttatgctta tttcatacaa gcgccgctgc agaaagttgt tgagaaaact ctgaaagcct
     1561 cggcacaaaa aaatgcaatc cttgtggaag gcgtagccgg tcttgaaacg ataaagatgc
     1621 tgggcgcgga aggccaaatc cagcgggctt gggaagaagc cgtcagttat atttcgaaat
     1681 ggggcaacaa gtcccgtatg atttcatctt cggttcagga tgtttcttat tttctacaaa
     1741 atctgatgac cgtcgcagtc gtcgttgccg gcgtctacat gatcaccgca ggttctctga
     1801 cttccggcgg actggtggcg ttggttattt tgtcccggca ggtgattgcc cccatggctc
     1861 aggtagtcaa tctggccacc cgatatcatc acgtcaggga agcattaaaa atgttagatg
     1921 atatcatgaa tctccctgtt gaacggcccg ccggaaaaac atttttaccg agaaagcgtt
     1981 ttgacggcgt aatcggcatc aaaaatcttt cattttccta tccgggacag acgaccatgg
     2041 tcctcaataa catctctctg gaaattgctg ccggagaaaa agtgggcatt atcggaccgg
     2101 tcggttcggg taaaacaact ctgggcaaat taatgctcgg gttgtatgaa ccggtcagcg
     2161 gcatggtaac catggacgga acggatatcc ggcagattga tccggcggaa ctgcgacatt
     2221 tccttggtta tgttccacag gatatcacgc ttttccaggg aaccctgcgc gacaatatta
     2281 caatgggcgt taataacgtc gatgaccaaa atgtgctgca ggcggcagaa atggccggca
     2341 ttatcgaatt cgccaaaata catccctccg gattcgatat gaaagtggaa gaattcggcc
     2401 gggggctttc cggcggtcaa cggcagtgcg tcgccatggc ccgcgctctc ctgctcgatc
     2461 ctccggtgct ggtccttgat gaaccgacaa gcaatatgga taaccgctcg gaaatcagac
     2521 tgaaacgata tcttgctcaa accgttaaag aaaaaaccat ggtgatcatc acccaccggg
     2581 catcgctttt ggatatggta acccgcctga tcgtcatcga tcatggatct attgtcgccg
     2641 acggcccgaa agatttcgtc ctcgaagcaa tgagaaaagg acagctgaac ttataaccgg
     2701 gagaatgcag gttgaagaag ctatttgatt tttttaaaaa taatcctttt gttgaatcct
     2761 tcagggatct tccggaaaca gataagatta gttttaaaga attgaaaaat aattttaaaa
     2821 tgaattattt attccgcaga ctgcctgaag aggacccgga cttcgccacg gatgtgcggg
     2881 agaccattct tgcccagacg cctcagggag gaagactgat tatctgggcc acactcatcc
     2941 tgctggtcat tttcatcgtc tgggcctctt tttcagaatt ggaagaggtt acgcggggcg
     3001 aaggcaaggt ggtcccgtcc agtcatgtac agatcgttca aaatcttgaa gggggaatta
     3061 tttccgaaat cctgatcaat gtcggagata aggtcaaaaa ggaccagctg cttctgaaga
     3121 tggaccccac gcgtttctct tcctctttcg aggagaaccg cgtcaaatac ctttccgaca
     3181 aggctaaagc agcccggctg aaagcggaag caaccggaag cgccctggtt attcctgaag
     3241 atgtcctgaa agaaagaccg gatattgcag aacgcgaacg tcagttgttc caaacccgca
     3301 gaatggaatt gggttcaagc tctgaaatca agcgccagca aattaatcag cggcgacagg
     3361 agctaaagga actggaggca aaacttgtcg agctcaatag aacctactct cttttacaaa
     3421 aagaaatcgg catgatcaaa ccgctggtta ataaaggagc ggcctccgat gtagaagttc
     3481 tccagcttga gcgtcaggcc agccagctgg aaggggaaat tgatcgtgtc cgtcatgcga
     3541 tccccgcagc acaggccaaa ttacaggaaa gtgaagtcgc cttgcgcgaa ctcagtctga
     3601 gctaccgcag caaatccagc acagaatcca gtgaagtact caaggaactt gatgaaagcg
     3661 cctcttcttc actggccttg aaagacaggc ttgacagaac cgccgtacgt tcaccggttg
     3721 acggcatcgt gaaccgcgtc atggtgaaaa cggtcggcgg cgtcgttcag ccgggaatgg
     3781 atctcgtcga gattgtaccc atgcacggca atcttctgat tgaagccaag atcaaaccgt
     3841 cagacatcgc ttttctccgt cccgggcaga aagccaccat taaatttaca gcctatgatt
     3901 ataccgttta cggggctctc gatgccgaac tggaatcggt cggtgcggac agcatcaccg
     3961 atgataaggg aaacagctat tttctggtcc gattaagaac ggataaaaat tatctgggta
     4021 cggcgcaaca tcccctgccg atcatgccgg gaatggttac aacggtagat attctcaccg
     4081 gcaagaaaac agttctatcc tatattttaa aacccgttct gaaagcacgg tatatggcct
     4141 tgagggaacg ttaacccgga ggatatttcg tttcctctcc tcgttgaata ggaatgttta
     4201 tgaccatcct cttaagcagc cccgtcccgg gagtaatgat cagatgggaa gcccatttga
     4261 aagggcactg caaaattaaa aaagccctgt ccatcgaaca gctcagaagc ctttgcggca
     4321 atcgtcagtt tgacgtcatt ctccttcacc ggatgctgat tgacatgtcc tccttccgga
     4381 ctttacggtc cgcatttccg gcagccagat tttttctgct tacagatcat ccggatgaag
     4441 aagaggccct gacgtttctt cgattgggga tagtgggcta tgccaatacg tacattgctg
     4501 caccgcgcct gcttgaggca ttacacgtca ttacggaagg aggcgtatgg ctgggacaga
     4561 aaattatcca gcgtctcata acaggcgtct ccaatacttt aaaaccggaa gacaaaaaag
     4621 atcaagtctc gctgcttccg gcaaacctga ccaaatccca acgcaacatt gccgaacgcg
     4681 ttgcccgagg acaatccaat ctggaaatcg cagccgattt gaacataacg gaaagaacgg
     4741 ttaaagcaca tctgacgtcc atctatgaaa aaacaaaaac cggcaacaga ttgaacctcg
     4801 ccctcttgat taatctgggc aaaaatcctt ccggttaaga ttaaagcttc ttcatgatat
     4861 gattgcaatc atgaagaata aaaaaagccg cgaaagtttg atttttcgcg gcttttttct
     4921 ttaaagcagt ttatgcgtca tggtcaatat cgaccttgcc gagcagcgag tctaaatttg
     4981 tcgcatccgt aatgctgtcg aaagtaacac tgcccaccag gtttccggcg gaatgatccg
     5041 tgctgctgtt gtaaacctcc agcacggctt tgccgtcgcc gttattatga aatcccagat
     5101 aggtcttggc aacggattcg tcggcaacat caaggattgc ggagatatcg actttgtctc
     5161 cgtctgtctg actgtaatct ttgacggtat cattggcctg agcgcttagc gtaaaggtat
     5221 cggcacccgc acctccggta agcgtgttac tccctgttcc accgctcagg atatcattac
     5281 cagaaccgcc actgagaata tcattacctt caccgccgta gatgatatcg ttaccttcct
     5341 gaccgtaaac gatatcgttt cccgctccgg cattaatgat atcattaccg ccggtaaggc
     5401 cgctttccgc tgaaagggtc aggtgattgg ccttgatatc agccatcatt tgcgatgttg
     5461 ttccggtata attgtaattt atcgtatcac cgaaaatgat atcatcgccg tctcctccaa
     5521 caatattgtc attgcctgca tcggacacca cggtactcgt aagatcggta agaacgctgc
     5581 tgagttgttc acctgttgta atgttggtcg ccacatcagg gttggaatcg gcagcttcac
     5641 cttcgacctg attcaggtgg ttcagattca tgtctgtaac attaatgccc accgcttcaa
     5701 tcgtaaatcc cttggcttcc agttgagcca cttcgctcac ggtatcgttt gtaccggtgc
     5761 caaggatctg tttaatgttc tcctgataga aataaacgcc atcaccgcct agctggctat
     5821 actcgtcacc tcgatagtaa taggttggtt ctccatcgga tacgaagatg gcctggctga
     5881 ccacgttgct ccccggtgag tagggtgcgc cgctgttcgc ccagacatag gccgtatgca
     5941 gagccgcctc gtaatttgtg ccgccttcgg gtccgctcgg tccattgtct atggcattga
     6001 ctgcattgat tgcgtcggtc aatgttccgg caacctttgc tccgttaatc ttgatatcga
     6061 aatatgttcc gctgttggga ttgatatcac gggcgtgagt atcaaaatca acaatgtgta
     6121 cacggacatt ttccgcaggt ccgttggcca gattattgag cgtattttcc acggacaatt
     6181 tcagcgccgc caatctggtc atatcaacgc cattaaactt gatcatggcg tccatgctcc
     6241 ctgatgaatc cagcatcaga acgagattca aggtctttcc ggcaatagac gtagcattca
     6301 aatcaccgac aagaacatca ttaccgccac tcccagttat gactccggca tatggggccg
     6361 catcatcaat ataatgatcg acggtttgag tggaaatatc atcggcattg gtaccgacaa
     6421 ccagtattcc gtccgtcggc agattgactg tcagtgtggc cgaggccgta tcgccgtcag
     6481 catccataac cttatacgta aagacatcac tcgccaataa cgttacactt gccggtcccg
     6541 tataggtgaa atctccggtt gagaaattga ccgccaaggt gccgcctgcc tgggtggaca
     6601 aggtgagcgt tgtcgtcgaa gtgttgtaac cggcagacga ggtatcataa atctcatcaa
     6661 tgccgtcacc gtttgcatca tgagcaatgg aaacgacttt cagaacagcc ggaccatcgg
     6721 cgccgaaagt tgcgggaaca gaacctgaaa taagattgcc cgtaatcgtg ctcggaacaa
     6781 tcaccgtatc caacaggacg tcatcaagct gagacaaatc cgtcacaaca atgggatcga
     6841 tttgtacggt attcagacca tcgtaagcaa tcggcgctaa atttgaagtc gtcacaccgg
     6901 tccccatgcc caaagcaaag gatgtaatat cgttgtttgc caggaaagtt tcccaggagg
     6961 cctgttccgc tgcgttgatc cccacggaac catatggtgc attcggttga ccatcagaga
     7021 gaaaatagga aatgttttgc gcccccgcaa tggctcccgg atcatcaaat gcagcctttg
     7081 cattgttaat agcagcgtca taattagtat tcccgcctgc cgacaaacca ttgattaccg
     7141 ctatgacatc atcggcggtc atccattgat cgccaacaat tgtcgatgac gagctgaagg
     7201 taaccagcct aaccatgacc ggtccattgt cctcataagc ctgaattaaa tcctcgagag
     7261 aatccttagc cagatccaga cgggtctttc cgtcaacacc tgaaggatcg ttcatggacc
     7321 ctgaaacatc gagggtaatc agcagattgg tcccgacaac ggtgcctgca tcaacggtga
     7381 tgctttgatc gcctgcactt gggatatcat cgacgatatc caccgtaatg gtccccgttg
     7441 ccgtgttgc
//
LOCUS       DAOSME010000087         7371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_11171_length_7371_cov_19.504237, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000087 DAOSME010000000
VERSION     DAOSME010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11171_length_7371_cov_19.504237"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PLG98_10110"
     CDS             <1..989
                     /locus_tag="PLG98_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE33657.1"
                     /translation="TDGALAVVLPSLDKKFDEIDIDEIKSRMVHVKTLPGEHLFAVTG
                     IPVRDGIFGGISCSHTVADGISLMLFLYAWMCITEGKPFLLPSRQKLFQGQPARSIEI
                     DKVFTPPLSELSDVIQKRVKRAAIKTYAKREYFSEEFLRDMKNRAKAENEKYSISDNQ
                     IITAYLLKKYHSVMMPKTDRIVLRTPVNFRDIHPDIDPLYIGNAQYYCVTVYGKDEID
                     SLSVYDIAFRLKESISSMRNENFLKELSQLSAYGIEIKTGLFKNDPPGNMDADILSSN
                     LTHLTDLEAMGVGSDVGSVLSISLAYYKTNFIILKEKSGRIFAEITSMYPLT"
     gene            1114..1278
                     /locus_tag="PLG98_10115"
     CDS             1114..1278
                     /locus_tag="PLG98_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33658.1"
                     /translation="MKNLRGVPPKRKGGEGNISGDTIREPFAYFEVPMEEDVVVKIFA
                     GCPPTGGDAV"
     gene            1477..>2297
                     /locus_tag="PLG98_10120"
     CDS             1477..>2297
                     /locus_tag="PLG98_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33659.1"
                     /translation="MPAPQIILELIERFERNIESYKSNGYNETQVRREFIDPFFEALG
                     WDVNNTAGHAEAYKDVIHEDAVKVSGVTKAPDYSFRIGGQRKFFVEAKKPSVNLKDAP
                     AAAFQVRRYAWSAKLPLSIVTDFEEFAVYDCRIKPDQKDKSDKARITYYSWRQYADKW
                     DEIAAVFSKDVILKGSFDKYAESSKAKRGTSEVDNAFLAEIESWRDALARNIALRNTL
                     SVRELNDAVQRTIDRIIFLRIAEDRGIEDYGSLRSLCNGANIYGRLQELFYKADDR"
     assembly_gap    2298..2397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2398..4065
                     /locus_tag="PLG98_10125"
     CDS             <2398..4065
                     /locus_tag="PLG98_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOE33660.1"
                     /translation="WHLQWYTENDPEKHAKAKRPILYKGYGDTWHLTTAERKRILLNN
                     IYGVDIDTQAVEVTKLSLLLKVLEDETSDTLNRQRVLFHERALPNLGNNIKCGNSLIG
                     PDFYDGKQMNMLDSEEAQRVNVFDWQAEFPEIFKAGGFDAVIGNPPYGALFNNEDKAY
                     LGRKFESVVGKYDSYGFFIEKGILLLQKEGCLGFITPHTWLTVIEAEALRKYLLTNAT
                     IESLVRLPTRVFSNATVNTILAFVKKGKKLNNNKQCIRVLLLPRDIAISEIKESMGEV
                     RHFTESSWATSEAHMFNIEVGTKEQKLVNKIKQAGVLLTELCDFCVGVQAYDSHTGQE
                     RSIIDLRIYHSTTKRDQTFRKELNGNDVSRYQIQWTEGNWISYGPWLAHPRQEYFFKG
                     PRILVREITGSGRYRIHAAFTDEDFVNYKTILNVIIKQESRSAGYHDFFILGLLNSEL
                     LSWYFPRSSNKLVTKTFPRISILDMKRFTAPLINFSNKAEKKRHDNMVRLVEQMLALN
                     KQLAEAKTGHEQTLIQRQIDATDKQIDKLVYELYGLTEEEIAIVESK"
     gene            4311..4739
                     /locus_tag="PLG98_10130"
     CDS             4311..4739
                     /locus_tag="PLG98_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33661.1"
                     /translation="MPKYKYKQKLKEYIARHGKPDALTEYFILQGFSEDEIKEGLEPY
                     IEEWEEAIYWIGRDDGSEYDWDLWKRSSLYEVLRHATTEQIEPYRERIEKADEKFRSM
                     TYEVKDSYGDLLDKNETINRDMHWWLFRIPIHGQVWRVCR"
     gene            complement(4852..5112)
                     /locus_tag="PLG98_10135"
     CDS             complement(4852..5112)
                     /locus_tag="PLG98_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33662.1"
                     /translation="MKLDLFFASLIGAAVGCAVAYGIIRLKGIWILGALLYIAGGIMA
                     FLFAHIIFWHLVADTHYVFIPGTVGFCIWSVFIARLFFPRHV"
     gene            5319..5450
                     /locus_tag="PLG98_10140"
     CDS             5319..5450
                     /locus_tag="PLG98_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33663.1"
                     /translation="MKNRLDMSNFQGRFNFADKQIDKLVYELYGLTEEEITIIEGGK"
     gene            5604..6080
                     /locus_tag="PLG98_10145"
     CDS             5604..6080
                     /locus_tag="PLG98_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOE33664.1"
                     /translation="MTIYKRFVGTWKLKSVVRRSSSGVVSKPFGDDPLGYISYMPDGF
                     MHAILMKSGRGRIGVSPEELSDAAATKKLLISWKYLAAGLKYVKAMTSFLSYCGTYDI
                     CGNVVYHHVQAAMFPDWIGTDLTRTFVFDEDRLTLSARDDAGNVMDLVWERVSIMM"
     gene            complement(6131..>7371)
                     /locus_tag="PLG98_10150"
     CDS             complement(6131..>7371)
                     /locus_tag="PLG98_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOE33665.1"
                     /translation="FITGGDQDITENIIHLALARIEGCVSGTKGISLFVVPKYWPDAN
                     GEKGEFNDVNCGGIEHKMGLRGSATAIVNFGEDNKCRGYLLGDPPVDGRGQGMAQMFQ
                     MMNEERTVTGLAAMSAATVAYNNACAYAAGRIQGRQLTNPKAGRVPIINHEDVRRMLM
                     FQKSITEASRAMIAFSYYCMDIAENTKDENERKLAKAYIEVNIPIVKAWCSDMAFLSI
                     SEAMQCYGGYGFSEEYPIAQQLRDCRIYPIWEGTNYIQSMDLIGRKWMMAGGQVFATW
                     LGEIDKFVQANKANAVLGAQVALLEESLNTYREIQNTMAGYLGQGKLGMIGFFATRIL
                     HATGYIYGAKLLLEQALICQKKADELGKEHFEYPFYAGKVASAKFFCHNILPVVSTIL
                     RVIKEGDNSVMEVPETTFAV"
BASE COUNT         2054 a         1701 c         1786 g         1730 t
ORIGIN      
        1 gcacggacgg cgccttggcc gtcgttctgc cgtctctgga taaaaaattc gacgagattg
       61 atatcgacga aatcaagagc agaatggtgc atgtcaaaac gttgccgggc gagcatctgt
      121 tcgccgtaac gggcataccc gtccgggacg gcatattcgg cgggatcagt tgttcgcata
      181 ccgtggccga cggcatttcc ttgatgctgt ttctttatgc gtggatgtgc atcacagagg
      241 gcaagccttt tttgcttccg tcccggcaaa aattatttca gggccaaccg gctcgctcca
      301 tcgagatcga caaggtattt accccgcctt tatcggaact cagcgacgtt attcaaaagc
      361 gcgtcaaaag agccgccata aaaacctatg cgaaaaggga atatttttcg gaagaatttc
      421 ttcgggatat gaaaaacaga gcaaaggcgg aaaacgaaaa atacagtatt tccgacaacc
      481 agattatcac ggcttatttg ctgaaaaaat atcacagcgt catgatgccg aaaacggacc
      541 ggatcgtgtt gagaacgccc gtgaatttcc gcgacattca tccggatatc gatccgcttt
      601 atatcggcaa tgcgcagtat tattgcgtga ccgtttacgg aaaggatgaa atcgatagcc
      661 tgtcggttta cgacatcgcc ttccgcctga aagaatccat ttccagcatg cgcaatgaaa
      721 attttctgaa agaactatcc caactctcgg catacggcat agaaatcaaa accggcctgt
      781 tcaaaaacga tcctccgggc aatatggacg cggacattct ttccagcaat ctgacgcacc
      841 ttaccgatct cgaagccatg ggcgtcggtt cggatgtcgg cagcgtttta tccatcagtc
      901 tggcttatta caaaacaaac tttattatcc tgaaagaaaa aagcggccgg atctttgctg
      961 aaataacaag catgtatccc ctgacgtgaa aggtattgaa gaatctttcg ggcataagac
     1021 taccggcaat ttgttattga taaaacatta aaaatctttt agtattcata accggaagaa
     1081 gtgaacaaca atttaataaa aaaggagaag attatgaaaa atttaagagg tgttccaccc
     1141 aaaagaaaag gcggtgaagg gaatatcagc ggcgatacga ttcgtgaacc ttttgcttac
     1201 tttgaagttc cgatggaaga agatgttgtg gtaaaaatct ttgccggctg tccccccact
     1261 ggtggtgacg cagtttaaca atctctttat gttaaaataa aaaaggcaga atcataaacg
     1321 attctgcctt tttttgttag tatgacgatg tccgcaatcc tttgtcgtcc gtttatatca
     1381 accttcagga aggggatcgg ggttgacagt tttactttca gagagtataa aaccacttca
     1441 atcaaaataa tatccaatgt taacaaggga cctctgatgc ctgcaccgca gatcattctg
     1501 gaactaattg aacgattcga acgcaacatt gaatcctata aatccaacgg gtataacgaa
     1561 acgcaggttc gccgcgaatt catcgatccc ttctttgaag cactgggctg ggatgtcaac
     1621 aatacggcag gacatgccga agcctacaag gatgtcatcc acgaagacgc cgtcaaagtc
     1681 agcggcgtca ccaaagcgcc cgactattcc tttcgcatcg gcggacagcg caaattcttt
     1741 gtcgaagcca agaaaccatc cgtcaacctc aaggacgcgc ccgccgccgc ctttcaggta
     1801 agacgttacg cctggtcggc caaactgccg ctttccatcg tgaccgactt tgaagaattc
     1861 gccgtttacg actgccgcat caagccggat caaaaagata aatccgacaa agcccggatc
     1921 acttactact cctggcgcca gtacgccgac aagtgggacg aaattgccgc cgtcttttcc
     1981 aaggatgtca ttctcaaagg ctctttcgat aaatatgccg aatccagcaa ggccaagcgc
     2041 ggcacgtccg aagtggataa cgcttttctt gccgaaatcg aaagctggcg cgacgcgctt
     2101 gcccgcaaca tcgccctgcg caacacgctg agtgttcgag aactcaacga tgccgtccag
     2161 cgcaccatcg accgcattat cttcctgcgc atcgctgaag accggggcat tgaagactac
     2221 ggcagcttgc gctctctttg caacggcgcg aatatttacg gccgtctgca ggagcttttc
     2281 tataaggccg acgaccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg
     2401 catttgcagt ggtacaccga aaacgatccc gaaaaacacg ccaaagccaa aaggccgata
     2461 ctctataaag gatacggcga cacgtggcac ctgaccaccg ccgaacgcaa acggattctg
     2521 ctcaataaca tttacggcgt ggatattgac acgcaggccg tcgaagttac caagctttcg
     2581 ctgctcttaa aggtgctgga agatgaaacc tccgatacgc ttaaccgcca gcgggttctt
     2641 ttccatgagc gcgcgctgcc gaatttgggc aacaacatca agtgcggcaa ttcgctcatt
     2701 gggccggatt tctacgacgg caaacagatg aacatgctgg attccgaaga agcccagcgc
     2761 gtcaatgtgt ttgactggca ggctgagttt cccgaaatct tcaaagccgg cggattcgac
     2821 gccgtcatcg gcaacccgcc gtatggggct ctattcaata atgaagataa agcatatctt
     2881 ggtcgtaagt ttgagtcagt tgtcggcaag tatgactcat atggtttctt catcgaaaaa
     2941 ggaattttgt tgcttcaaaa agagggttgc ctgggattca ttactccaca cacgtggctt
     3001 actgtgattg aagcagaggc gctgcgaaaa tatcttctga ctaatgctac aattgaaagt
     3061 ttggttagac taccgactcg cgtgttcagt aatgccaccg tcaacacgat tttagcgttt
     3121 gtaaaaaaag ggaagaaact aaacaataat aaacagtgca tcagggttct tcttctgcct
     3181 cgtgacattg caatctcaga aattaaagaa agtatgggag aggttcgcca ttttaccgaa
     3241 agttcgtggg caactagcga ggctcacatg tttaacatag aagttggtac taaagagcag
     3301 aaactagtca ataagataaa gcaggccgga gtgctattga cagagctttg tgatttttgt
     3361 gtaggtgtcc aagcatatga ttctcacacg ggacaagaaa gaagcattat tgatttacgt
     3421 atatatcatt caacaacaaa aagagatcaa acatttcgta aagagcttaa tggaaatgat
     3481 gtctcccgtt atcaaatcca gtggactgag gggaactgga tatcttatgg tccatggctt
     3541 gctcatcctc ggcaagagta tttctttaag gggcctcgca ttcttgttcg tgaaattaca
     3601 ggttctggac gttacagaat acatgcggca ttcacggacg aagattttgt taactataag
     3661 acaatcctga atgtgattat taaacaagag agtcgcagtg caggttacca cgactttttt
     3721 atccttgggt tgctgaattc agaactactt tcatggtact ttcctagatc aagtaacaag
     3781 ttggtcacta aaacatttcc acgcatttca atactcgata tgaagcgatt cacggctcca
     3841 ctaattaatt tctctaacaa ggcagaaaaa aaacgtcacg acaacatggt gcgactcgtt
     3901 gagcaaatgc ttgccctgaa caaacaactg gccgaagcca aaaccggtca cgaacaaaca
     3961 ttaatccagc gccagattga cgcgaccgac aaacaaattg ataaattagt ttacgaactc
     4021 tacggcctga cggaagaaga aattgctatt gtggaaagca aatgaagaat catcggacgc
     4081 aagaagtccc acggcgctcc gcgcctggga tagttccact tacgtttcga gcagcactac
     4141 gcttgctctc aacgagtgtc actaaaataa agatagtcga agggaaataa aatttggggc
     4201 gtgctcaggg gagcacgccc tacaaaaaaa tggaaacaca attttgaaat gtagggattg
     4261 attcccgatc aatccgtaaa aactacgatg tttctaaggg attgttacca atgcccaagt
     4321 acaaatataa acaaaagcta aaagaatata ttgccaggca cggaaaacct gatgcgctga
     4381 cggaatactt catccttcaa ggtttctcgg aagatgaaat aaaagagggg ctggagccgt
     4441 atattgagga atgggaagaa gccatctatt ggattggaag agacgatgga tcggaatatg
     4501 attgggatct gtggaaacgc agctcattgt atgaggtatt acgccacgcc acaaccgagc
     4561 aaattgagcc ttatcgggaa agaatcgaaa aggctgacga aaaattcagg tcaatgacat
     4621 atgaagtcaa agattcttat ggcgatctgc ttgataagaa tgagaccata aacagagata
     4681 tgcattggtg gctgttcagg ataccaatac atggccaagt atggagggtt tgccgataat
     4741 tatgagcaag ataggagaat aaaaaacggc atgcggtggg gcctcccaca aaccccactt
     4801 tacgcgcccc cgctggaagc ggggtgctac ggccgggaga atattaccgt gttacacatg
     4861 cctgggaaaa aataagcgag ctataaaaac cgaccagatg cagaagccaa ctgtgccggg
     4921 gataaaaaca taatgtgtat cggcaaccag atgccaaaaa ataatatgcg cgaagagaaa
     4981 agccattatt ccgccggcga tataaagcag cgcgccgaga atccagatgc ccttgagccg
     5041 gatgattccg taagcgacgg cgcagccgac tgccgcgccg ataagagagg caaaaaagag
     5101 gtctaatttc atgatcgttg attccccacg ttgtgcaatc gacgtgatgt aaggcgtttt
     5161 gcccgtgtcc ctaattcggc tacaagttta gacaatgcat ccaaagaagt caagaacgac
     5221 tttggtaaca cgagcagaca ggactctgct gagtttaagt ttcagactgc acttgttaac
     5281 aagatgtccg aaacgatcat ggagaccgaa aaaaaatcat gaaaaatcgg cttgacatgt
     5341 cgaattttca aggtaggttc aattttgccg acaaacaaat cgacaaactg gtctacgaac
     5401 tctacggcct gacggaagaa gaaattacga taattgaagg cggtaaataa atatggattc
     5461 ccgccaaaac catgcgggaa tgacagtgcg gggttaattg ttacctaccc aagtaaatga
     5521 aagtgtaggg attgatccac cgatcaatcc gcaaaaaaac ggcgcgctct ggagttgcgc
     5581 cctgaaattt agggatgaga tttatgacga tctataaacg gtttgtggga acgtggaaac
     5641 ttaaatcagt tgtaagaagg tcttcttccg gcgttgtgag taaacccttc ggcgatgatc
     5701 ctctcggata catttcctat atgccggacg gcttcatgca cgccattctg atgaagtccg
     5761 gacgaggccg catcggtgtt tctccggaag agctcagcga tgccgccgca acaaagaagc
     5821 ttttgatttc ctggaaatat cttgcggctg gattgaaata cgtcaaagcc atgacgtcat
     5881 ttttatccta ctgcggcacc tatgacattt gcggcaacgt tgtctatcat cacgtgcagg
     5941 ctgccatgtt tcccgactgg atcgggacgg atctgacgcg tacgtttgtt ttcgacgaag
     6001 acagactcac gctgagcgcc cgtgacgatg cggggaatgt gatggatctg gtctgggaac
     6061 gcgtttcaat aatgatgtga aaaaaaagag ggacggggag aacgcccgtc cctcctgatg
     6121 aacctcggtt ttacaccgca aaagtcgttt ccggaacttc cataacggag ttgtcgcctt
     6181 ccttaattac tctcaaaatc gtactaacca caggcaggat gttatggcag aagaatttgg
     6241 cggatgccac cttgcccgca taaaacggat attcaaagtg ttctttgcca agttcatcag
     6301 cctttttctg acagatcagc gcttgttcga gcagaagttt tgcgccgtag atataccctg
     6361 tggcgtgcag gatgcgggtg gcgaaaaagc cgatcatgcc cagtttgccc tggcccagat
     6421 agccggccat ggtattttga atttccctgt aagtgttcag cgattcttct aaaagagcga
     6481 cctgcgcacc cagcacggca ttggctttgt tggcctgaac gaatttatcg atttcgccga
     6541 gccacgtcgc aaaaacctgg cctccggcca tcatccactt gcgtccgata agatccatgg
     6601 actggatgta attggtgcct tcccagatcg gataaattcg gcagtcacgg agctgctggg
     6661 cgatggggta ctcttccgag aaaccgtagc cgccgtagca ctgcatggct tcggagatgg
     6721 aaaggaacgc catgtccgaa caccaggcct tgacgatggg gatgttgacc tcaatgtatg
     6781 ccttggccag cttgcgttcg ttctcgtcct tagtattctc cgcgatatcc atgcagtagt
     6841 aggagaaggc aatcatggcg cgggaggctt ccgtaatgga tttctggaac atgagcatgc
     6901 ggcggacgtc ttcgtggttg atgatgggga ctctgcccgc cttgggattg gtgagttggc
     6961 ggccctgaat acggccggcc gcatacgcgc aggcgttgtt gtaagcgacg gttgccgcag
     7021 acatggcggc aagaccggtg accgtgcgtt cttcattcat catctggaac atctgggcca
     7081 tgccctgtcc cctgccgtca acggggggat cgccgagaag atacccgcgg catttgttgt
     7141 cctcgccgaa attaacgatg gctgttgccg agcctcgcaa acccatcttg tgttcgatgc
     7201 cgccgcagtt gacgtcgttg aactcgccct tttcgccgtt ggcgtccggc cagtacttgg
     7261 gaacaacaaa cagggagatg cccttggttc ccgagacgca gccttcaatt cgggcaagcg
     7321 ccaggtggat gatgttttcg gtgatgtcct gatcgccgcc ggtgatgaaa c
//
LOCUS       DAOSME010000088         7328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_11256_length_7328_cov_20.528805, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000088 DAOSME010000000
VERSION     DAOSME010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11256_length_7328_cov_20.528805"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLG98_10155"
     CDS             <1..306
                     /locus_tag="PLG98_10155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE33666.1"
                     /translation="TELSNSADDPDVSIARARVEPGVTTRSHRLKGITERYFIISGRG
                     AVEIGELPPQEVNPGDIVLIPPLCPQRITNIGKNDLIFLAICSPRYMQDAYEDVEDF"
     gene            complement(316..2724)
                     /gene="priA"
                     /locus_tag="PLG98_10160"
     CDS             complement(316..2724)
                     /gene="priA"
                     /locus_tag="PLG98_10160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOE33667.1"
                     /translation="MFVEIALNIPSDKLFTYEVPAELEQDIVVGKRVFVPFGNKKRTG
                     FITDIRHSCGLNNIKSVAEVLDDEPLFHTSDLNFYKWIAGYFIYPLGKTLAELIPAGP
                     EKKDFLWITPLISETDLHLTPAQNTLMAFLKQHPRGTTLTGIAKISGLKNALSAAKKL
                     QQAGLVSLEKKHGKPLSPQSEKMVALDEQKLPDVKLTGKQRMLVEWMQAKGSVSWGEL
                     IEGSGVTGAVLKALLDKGLIHLTKQEKIRTASFDSHIGRHKNATVLNDRQNAAMEEIS
                     RYLEKNIFTPILLHGVTGSGKTEIYLKAIENVLQTGGSAMYLVPEIALTPQLISRVAG
                     RFDRERIAILHSGIAESVRYDQWRQIRRGRIHLVIGTRSALFAPLTNLRLIIVDEEHD
                     TSYKQDDRLCYNARDLAVVKAKMSSAVVIAGSATPSIHTYFNAQTQKYHYLELPGRID
                     DKPMPVVEIVDMKLQQAKAGKVPVLSDSLIEQIDNTVRNKEQVLLFLNKRGFDTFLVC
                     ADCGYSFRCPNCAVSLKNHLAEGIVKCHYCDYTIQSMPLCPSCKSSRILSYGTGTQKL
                     EKEVQKFFPDARIDRMDSDTTTRKGSQEKILQALDKREIDILIGTQMITKGHDFPFIT
                     LVGVVSADTSLNMPDFRAAERTFQLITQVAGRGGRGSAPGRVIIQTFNPDHYALRHAQ
                     NHDYKSFYREEIKFRLDLQYPPFGRIINLRLSSIKKDVLIEEAKRLGKLAKKLCAGYG
                     NTVEIIGPAESPLSKLRGRFRWQMLIKGKDINALHQIAKDVMHKNKNTQIKMMADVDP
                     ESFM"
     gene            complement(2900..3286)
                     /gene="dksA"
                     /locus_tag="PLG98_10165"
     CDS             complement(2900..3286)
                     /gene="dksA"
                     /locus_tag="PLG98_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOE33668.1"
                     /translation="MEKQINVKQKQEKMKAYKAMLTQKINELLSEAGKTVSEMTNGKE
                     NYPDPTDRASLESDRNFELRIRDRERKLIMKMQEAIKRIDDGTFGICEVCGGPISEKR
                     LMARPVTTLCIDCKTKQEKMEKLKGE"
     gene            complement(3416..3910)
                     /gene="rfaE2"
                     /locus_tag="PLG98_10170"
     CDS             complement(3416..3910)
                     /gene="rfaE2"
                     /locus_tag="PLG98_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOE33669.1"
                     /translation="MNKILDRTTLQRKLDTLRQKGRKIAFTNGCFDILHVGHVRYLRE
                     ASKTGDVLVLALNSDSSVRAIKGEKRPLVPATERAEILAALDFIDFITIFDELTPLDV
                     IKSLKPDILIKGGDWPEEKVVGREEVKKWKGKVVIIPEVQGKSTTNIVEKILQVYGPQ
                     NQKG"
     gene            complement(3928..4671)
                     /locus_tag="PLG98_10175"
     CDS             complement(3928..4671)
                     /locus_tag="PLG98_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOE33670.1"
                     /translation="MNLAEAFNDLSQVIFPPQCLGCAEIIPSVKNQIFCRDCAKHVHF
                     IAGSICPVCGTTFPGSPAENHLCEDCLKKQPYFSQARAVFCYEAVILDAIHQFKYKRD
                     IATGEILADFMADYPFPDIAFDDYSLIVPVPLHVKRLRERGFNQSLILARRLAKRKQI
                     PVDFLVLKRHRFTSTQTQSNKQERKINIRGAFEVRGIKTIAGRNILLIDDVFTTGATV
                     NECSKTLMKAGAKKVSVLTLARVLQHVSF"
     gene            complement(4668..5063)
                     /gene="rsfS"
                     /locus_tag="PLG98_10180"
     CDS             complement(4668..5063)
                     /gene="rsfS"
                     /locus_tag="PLG98_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOE33671.1"
                     /translation="MPAKKNVTRERLILCINAVLQKKAKDIIVLNLSKLSAFTDYMII
                     CSGTSEREVQAIAQAIQLFLKKADIRPLGVEGEANGQWVLLDYDDVVISVFYEPFRTF
                     YDLENLWDAPRMQVDENKKEIKRLSREMA"
     gene            complement(5476..5808)
                     /gene="trxA"
                     /locus_tag="PLG98_10185"
     CDS             complement(5476..5808)
                     /gene="trxA"
                     /locus_tag="PLG98_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE33672.1"
                     /translation="MNKQDIKHAGDGDFEKTVLKNDKLVLVDFWAPWCGPCRAIGPIL
                     EELAGEYGDRIDIVKVNVDDNPQTASQFGIKSIPTLLVMKGGTVKDTSIGLSSKDKLA
                     DMIKRHIH"
     gene            6017..6628
                     /locus_tag="PLG98_10190"
     CDS             6017..6628
                     /locus_tag="PLG98_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE33673.1"
                     /translation="MASQALHKKFKQGDLLIGEEDPIRAFYVVTAGQLKLYKSSSEGR
                     EQTLSLLKPGDPFGLCTAFATESFPANAMAIEKSAVLIIPGELMENVAKKEPVLLLNI
                     IQILSQRLKESMTLIESLSLKEIPARLSTYILDALSRTSEDKRNKLELTISHRELSKM
                     LGATPEALSRAFKKMSNDKILKVEGRVITVLNREEMKTLAEGD"
     gene            complement(6662..>7328)
                     /locus_tag="PLG98_10195"
     CDS             complement(6662..>7328)
                     /locus_tag="PLG98_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE33674.1"
                     /translation="SWGFSCLIQHEGQNILFDTGGDGLVLAANMAKLGIDPASLDLLM
                     ISHQHWDHTGGIYYILKEKRNLRICLPHSFSARFKEDMKRYGAEVIDINEAREVITGL
                     HSTGDMEALIREQSALLKTQAGTVVITGCAHPGIVHIAETAKRILPDDALALVMGGFH
                     LLDNADEDILKIIARFRELGVRYAAASHCSGPNARELFARKYGEHFIRLGAGSTIALE
                     DLR"
BASE COUNT         1785 a         1741 c         1711 g         2091 t
ORIGIN      
        1 acggaattat ccaattctgc cgacgatcct gatgtatcca ttgcccgggc aagggtggag
       61 ccgggcgtga caacgcgcag tcatcggctg aaaggaatta cggaacgtta ttttatcatc
      121 agcggcaggg gagctgtgga aatcggagaa ctgccgcccc aggaagtcaa ccccggcgat
      181 atagtcttga ttccgccctt atgtccgcag cgcattacca acatcggcaa aaacgatttg
      241 atttttctgg ccatctgctc gccgcgttat atgcaggatg cgtatgaaga tgtcgaagac
      301 ttttaaccct gatgattaca tgaagctttc cgggtccaca tccgccatca tttttatttg
      361 cgtgttttta tttttatgca taacgtcttt tgcgatctga tgcagggcgt tgatgtcttt
      421 ccctttgatc agcatctgcc agcggaagcg cccccggagc ttggaaaggg gagattccgc
      481 cggaccgata atttctaccg tgtttccata ccccgcgcac agcttcttcg ccagtttgcc
      541 cagacgtttg gcttcctcaa tcaatacgtc ttttttgatc gaagacaggc gcaaattgat
      601 gatccggccg aacggcgggt attgcagatc cagacgaaat tttatttctt ccctgtaaaa
      661 ggatttataa tcatggtttt gagcgtgcct cagcgcatag tggtcgggat tgaacgtttg
      721 aataatgact cggccgggtg cgcttccccg tcctcctcgt ccggcgactt gcgtgattaa
      781 ctgaaacgtt ctttcggcgg cgcggaaatc cggcatgttc agggaagtgt ccgcggaaac
      841 aacgcccacc agggtaatga acggaaagtc gtgtcctttg gtgatcattt gcgtgccgat
      901 gagaatgtca atttcccttt tgtccagtgc ctgaagaatt ttttcctgag accctttacg
      961 ggtggtcgtg tcggagtcca ttctgtcaat ccgggcgtca ggaaagaatt tctgcacttc
     1021 tttttccagt ttctgcgtgc ccgttccgta acttaaaatg cggctgcttt tgcaggaagg
     1081 gcaaagcggc atcgattgaa tcgtatagtc gcagtaatgg catttgacga tgccttccgc
     1141 aagatgattt ttgagggaga cggcgcaatt gggacagcgg aaactatatc cgcagtcggc
     1201 gcaaactaaa aatgtatcaa acccgcgctt gttgagaaaa agcagaacct gttctttgtt
     1261 ccggacggta ttgtcgattt gctcaatcaa actgtccgaa agcaccggca cttttcccgc
     1321 tttcgcctgc tgtaatttca tatcaacaat ctcgacgaca ggcatcggtt tgtcatcgat
     1381 tcttccggga agttccagat aatgatattt ttgggtctgc gcgttgaaat acgtatgaat
     1441 gctcggcgta gcggaaccgg cgatgacaac ggcggaagac atttttgcct tcacgacggc
     1501 caggtcccgg gcattatagc agagccggtc atcctgcttg taagaagtat cgtgttcttc
     1561 atccacgatg atcaatctca aattggtcag cggagcgaaa agagcggaac gggtgccgat
     1621 taccagatga atgcgaccgc gtctgatctg ccgccactgg tcatagcgta cggattcggc
     1681 gatgccgctg tgcagtatgg caatgcgttc ccggtcaaac cgtccggcaa cccgcgaaat
     1741 cagctgaggc gtgagggcta tttccggtac gagatacatg gcagagccgc cggtttgtaa
     1801 aacattttca atggctttta aatagatttc cgttttcccg ctgccggtta caccgtgaag
     1861 cagtatgggc gtgaaaatat ttttttccag atagcgtgaa atctcttcca ttgccgcatt
     1921 ctgccggtcg ttgagaacgg ttgcgttttt atggcgtccg atgtgcgaat cgaatgaagc
     1981 ggttcggatt ttctcctgtt tcgtcagatg gatcagacct ttatccagta aggctttgag
     2041 cacggcgccg gtaacgccgg aaccttcaat caattctccc catgataccg agccttttgc
     2101 ctgcatccat tcgacaagca ttcgctgttt gccggttaat ttgacgtcgg gcagtttttg
     2161 ttcgtcgagc gcaaccattt tttcgctttg cggggacaac ggtttgccgt gttttttctc
     2221 cagagagacc aatcccgcct gctgaagttt ttttgccgcc gaaagagcgt ttttcaggcc
     2281 gctgattttc gcgatgccgg tgagcgtcgt gccccgcgga tgttgtttca ggaacgccat
     2341 cagtgtattt tgtgcaggcg ttaaatgcag atcggtttcc gatatcagcg gggttatcca
     2401 gagaaaatct tttttttccg gtcctgccgg aatgagctcg gccagggtct tacccagcgg
     2461 ataaataaaa tagccggcaa tccatttgta aaagttcagg tcgcttgtat gaaacagcgg
     2521 ctcatcgtca agcacttccg caacggattt gatattgttc aggccgcagg aatgacggat
     2581 atcggttatg aatccggtgc gttttttatt gccgaaagga acaaagacgc gtttgccgac
     2641 gacaatgtcc tgctccagtt cagcgggaac ttcatacgtg aaaagtttat ccgatggaat
     2701 gtttaaggcg atttctacaa acataactat tccatgatgt tcaaaaatat accgttagta
     2761 accaatcctg tccggaaaat ccatttttct gataccatct ttagtccaaa aaggaaatta
     2821 tttatcggta gctatagttt taaaaataaa aaaacgctcc ggttggggag cgttttttat
     2881 caagcggaat attttaaatt tattcgcctt tgagcttttc catcttttcc tgttttgttt
     2941 tacaatcgat acagagggtc gtgaccggtc ttgccatcaa tcttttctct gatatgggac
     3001 cgccgcagac ttcgcagata ccgaaagtgc cgtcatcgat ccttttaatg gcttcctgca
     3061 ttttcatgat cagtttgcgt tctctgtccc ggatacgcaa ttcaaaattt ctgtcggatt
     3121 caagagacgc cctgtcggtg ggatcgggat agttttcttt cccgttggtc atttccgaaa
     3181 cagttttgcc ggcttcgcta agcaactcgt tgattttttg agtcagcatg gctttgtaag
     3241 ccttcatttt ttcctgtttc tgttttacat ttatttgttt ttccaaaatc atcactcctt
     3301 gcaagaacaa cttggccgtc aaatctacat gatgaaaatt tatttgtaaa gagaaatttt
     3361 tgtccgcagt ttttttcagg caatatgcct caattcaatg atagtatgaa taaaatcaac
     3421 ccttttggtt ttgcggacca taaacctgca ggatcttttc cacaatgttg gtggtggatt
     3481 taccctgcac ttcgggaata atgacaactt tgcctttcca tttcttaact tcttcgcggc
     3541 cgacaacttt ttcttccggc cagtctcctc ccttgatcag gatgtccggc ttcagggatt
     3601 tgatgacgtc cagaggcgtc aattcatcaa atattgtaat aaagtcgatg aagtcgagcg
     3661 cggcaaggat ttccgctctt tctgttgccg gaacgagagg tcttttttct cctttgatgg
     3721 ctctgactga tgaatcgctg tttaaagcca gaaccagcac atcgccggtt ttgcttgctt
     3781 cgcgcaggta gcggacatga ccgacatgca gaatatcaaa acatccgtta gtgaaagcga
     3841 ttttccttcc tttttgccgc agtgtatcca gctttctttg cagggttgtc ctgtctaaaa
     3901 ttttattcat taccgctcct ttacgtttta aaatgagaca tgttgtaaca ctctggccag
     3961 ggtcaatacg gaaacttttt ttgctccggc cttcatcagg gttttggaac attcattaac
     4021 ggtggcgccg gtagtaaaga cgtcgtcgat gagcaggata ttgcgtccgg caattgtttt
     4081 tatccctctg acttcaaaag ctcctctgat atttatcttt ctttcttgtt tattcgactg
     4141 agtctgggtt gacgtaaatc tgtgccgttt caacaccaag aagtcaacag gaatttgttt
     4201 tctcttcgcc agacggcggg ccagaataag cgattgattg aaccctcttt cgcgcagtct
     4261 tttaacatgc agaggaacgg gaacaatcag cgaataatcg tcaaaagcaa tgtccggaaa
     4321 aggataatcg gccatgaaat ccgccagaat ttcccctgta gcgatatccc gtttgtattt
     4381 aaattgatga atcgcgtcga ggatcaccgc ttcgtaacaa aagacggccc tcgcttgaga
     4441 aaaataaggc tgtttcttca gacaatcttc gcacagatgg ttctccgccg gactgccggg
     4501 aaaggttgtg ccgcaaacgg gacagatgct gccggcaata aaatggacgt gtttagcgca
     4561 atcccgacaa aaaatctgat tcttcacgga cgggatgatt tcagcgcatc ccagacattg
     4621 aggcggaaaa attacttggc ttaaatcatt gaacgcttca gccagattca tgccatttct
     4681 ctgcttaacc tttttatttc ctttttgttc tcgtcaacct gcatccgggg agcatcccag
     4741 agattttcaa gatcgtaaaa cgttctgaac ggttcataaa aaacggaaat gacaacgtcg
     4801 tcgtaatcca gcagaaccca ttggccgttg gcttcgcctt cgacgcccag aggtctgatg
     4861 tccgcttttt tcaggaataa ttgaatggcc tgggcgatag cctggacttc cctctccgac
     4921 gttccgctgc agatgatcat gtaatctgtg aaagctgaaa gtttgcttaa atttaaaaca
     4981 atgatgtctt ttgctttttt ttgtaataca gcgttaatac acaaaatcag gcgttctctg
     5041 gtcacgtttt tctttgctgg caaataaacc tcctgtccgg cactgaattt tccgaatata
     5101 tgccacaaat gacggaaaat gacaactttg ctgttttttt gcatgaagat tataaaaaat
     5161 cgtcatgggg tattcaggac agtttctgtt ttctttgcac atgtttttac acaggatgaa
     5221 ccccttgcgt ccgattcgag aaacaggccc tgacgttgtt tatgaaacaa tatttacccg
     5281 tttaaagcag tttctgcttt cgtgttaaga atcggttgat gttttttctt tctttctgtc
     5341 ctcgaccgga cagccgggtt tcattcaggt ggatacaccc aatttaggca agagccatcc
     5401 ctgcaacaaa aaccataccg caacaatgac ggccaatatc aaaaaatcat tcatttcctt
     5461 tgccccctgg tgttatcagt ggatatgccg tttaatcata tcggctagtt tatccttcga
     5521 ggacaaaccg atcgacgtgt cctttaccgt tccccctttc atgaccagca gtgtcgggat
     5581 gcttttgata ccgaattgac tcgcggtttg aggattgtca tccacgttaa ccttcacaat
     5641 gtctatccgg tcgccgtatt ctccggccag ttcctccaga atcgggccga tcgcacggca
     5701 aggtccgcac cacggcgccc agaaatccac taaaaccagc ttgtcatttt ttaaaacggt
     5761 tttttcgaaa tcaccgtcac cggcatgctt gatgtcctgc ttattcattt gctcccctcc
     5821 cttgatataa aagtatttga tttatcaatc cgcaattgtt aaatattcgt atattcataa
     5881 tcgcaagcat atgtcccggt ttgtggaatg tcattgatct gaatcaatgt ggagaaaaat
     5941 atttgccatg gaacttctta aacaaataca gaactttggg ctgtttcaag gcctgtcggc
     6001 ggagaacttg aaactcatgg catcgcaggc gttgcataaa aaattcaaac agggtgattt
     6061 gctgatcggg gaggaagatc ccatccgggc gttttacgtg gtgaccgccg ggcagttaaa
     6121 gctttacaaa agttcatccg agggcaggga acagacgtta tcgcttttaa aaccgggaga
     6181 tcctttcggt ctttgtacag cctttgccac ggaaagcttt cccgccaatg ccatggctat
     6241 tgaaaaaagc gcagtattga ttattccggg agaattgatg gagaatgtcg ctaaaaaaga
     6301 accggtgctg cttctcaaca tcattcaaat actttcccag cgcctcaagg aatccatgac
     6361 gctgatcgaa tcgctttcgc tgaaggaaat accggcaagg ctgtcaacct acattcttga
     6421 cgcactttca aggacgtcag aagacaagag aaacaaactg gaattaacca tatcccaccg
     6481 ggaattatcc aagatgcttg gcgcaacgcc cgaggcgctt tcccgtgcgt tcaagaaaat
     6541 gagcaacgat aaaattctga aggtcgaggg acgcgtcatt accgttttga acagggaaga
     6601 gatgaaaacg ctggccgagg gcgattaagt tattccagtg ctaaacattg atgatccggg
     6661 gttatctcaa atcctccaga gcgatcgtac tgcctgcgcc gagacgaatg aaatgctctc
     6721 cgtatttgcg ggcgaagagc tcgcgggcat taggaccgct gcagtgagag gcggccgcgt
     6781 aacggacgcc gagttcccgg aaccgggcga tgattttcaa aatgtcttcg tcagcattgt
     6841 cgagcaaatg aaaacctccc atgaccaggg ccagtgcatc atccggtaaa atacgcttcg
     6901 cggtttcggc aatatgcacg atgcccggat gcgcacatcc ggtaatcacg acggttcccg
     6961 cctgcgtttt cagaagggcg ctctgttccc ggatcagggc ctccatgtcg ccggtactgt
     7021 gcagaccggt gatcacttct cgtgcttcat ttatgtcaat cacttctgcg ccgtagcgct
     7081 tcatatcttc tttgaagcgg gcggaaaacg aatgaggcag acaaatccgg agatttcgtt
     7141 tttctttcag aatataatag atgcctccgg tatgatccca atgctggtga gagatcatca
     7201 gaagatcaag cgatgccgga tcaatgccca gctttgccat atttgccgcc aggacaagac
     7261 catcgccgcc ggtatcaaaa aggatgtttt gcccttcatg ctggatcaga caggaaaatc
     7321 cccacgag
//
LOCUS       DAOSME010000089         7221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_11473_length_7221_cov_22.268351, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000089 DAOSME010000000
VERSION     DAOSME010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11473_length_7221_cov_22.268351"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..931)
                     /gene="yihA"
                     /locus_tag="PLG98_10200"
     CDS             complement(329..931)
                     /gene="yihA"
                     /locus_tag="PLG98_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOE33675.1"
                     /translation="MKITSAQFIKSAVWPPQYPPAIMPEIAFVGRSNVGKSSLMNTLV
                     GRKNLAKTSNTPGRTQLINFFTVNETISFVDLPGYGFAKVPRSVKKDWGDMMEAYLRE
                     RQSLCLVIFILDIRRDPNADDLSLRDWLDHYRIPYLYILTKSDKLSNNQAIARKRAIE
                     KMLRISSEKKTILFSAKTQKGKVEILEFLENHLRALDKHS"
     gene            complement(970..1863)
                     /locus_tag="PLG98_10205"
     CDS             complement(970..1863)
                     /locus_tag="PLG98_10205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE33676.1"
                     /translation="MISVIIPTFNRGYTLAQVLDSYYRQQFVAELIIVDDGGSDKTAV
                     VVDEFVRKYPRIKTMYLKNEKRQGAPYSRRRGLLAADGEFVLFGDDDDFLEENYTTVC
                     LNKLKSLSADIVSGRHFFRTPGETVAEAIARFGQGSIDVPPFDFRLFYINTDAKFKSD
                     VELPFTHAIYLVRRELLLSYEMDTFYSRGNGFREENDIQAKIHINGGKIIITNDTHSV
                     HMHSSEVQTGGQRVGALAKLFWSLYYTAYFFKKYYKPIMERQGENGCYLKGIVIYYAL
                     TLLTFPFLVLKEKRILRSMRK"
     gene            complement(2207..3589)
                     /locus_tag="PLG98_10210"
     CDS             complement(2207..3589)
                     /locus_tag="PLG98_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE33677.1"
                     /translation="MPSILVIDDDASIAETLDLYLTEEGYNVRTALTGTEGLNQYVQE
                     PSDVVILDIRLPDIDGFTVLEDLKEENENVKVIMITAFHDMETTIQAMKSGAFDYIHK
                     PVNVDELDLAIKKALKSLEMEKKIDGLLMEPSRSFRVGDIIGTGNEMREIFKIIGTVS
                     QSRTTVLIQGESGTGKELIAKVVHNNTSPDEPFIAVNCSAIVETLLESELFGHEKGSF
                     TGAVARKLGKFELARFGSVFLDEISEMSVNLQAKLLRVLQEMEFDRVGGKEKVKVNAR
                     IMAATNKDLKALVKEGKFRDDLYYRLNIVSITLPPLRARRQDIPPLIDYLLTKINLDL
                     HKKIIGVSDEVMEIFMQYTWPGNVRELENLLVRACVVAKGQVLGVNDFPELGKEEAAG
                     PAGDRDANLFQASEPGKLLTLDEVEERYIRKVIKETDKNKGEICEILGVSRPTLERKL
                     EKYGITFERE"
     gene            complement(3602..6214)
                     /locus_tag="PLG98_10215"
     CDS             complement(3602..6214)
                     /locus_tag="PLG98_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33678.1"
                     /translation="MTLFSKFKILLYKAAQLRILFASVTVVILFLLLALTVNNSREKD
                     IVNIFSRQQLVNVQNTSKRMMDVFSQIGKNIDLFSRLHPDLTIDEKELDSYYKMLSSG
                     WEDTFDAIVMFDAGGNIKNVYPKNVVPSINVSEHFNVLSKQQKRYLGLALPDASGTLG
                     SKQKHDRYLVLGYPLRKPNGEFSGALLISFSLSAVLHKYRQRPADDELGELWLIDEQQ
                     KILVHPRWAFVGNDIKKFLKKSDEQASMFSSENGDNFEAVVLQGDKIKQRSIISYYPV
                     KAGDKKWTMLVVAPYSKVISPLRKTFFYTLFSSLLLIIIVIATSMSFAYKEGKRLRHR
                     EERKRLKERRDWQEKLLREKKTIEGIIEGSPIPSFVINNKHKVILWNRACSELTGHSA
                     DEMLGTGNHFKPFYSVTRPMIADLIVDSDMDGLSKHYGTKNVKKSEKVVGAYEATDYY
                     ANLGGQSRYLYFLAAPIYDEEGKIIAAVETLQDVSREREMTKSLSEYAETLQNELIEN
                     IELQRQIQDLNNYLQTIITSLPDRIYEIDEKGIINFMSRGLRKKTGTSGEFKNKYFLD
                     FVASGYEDLVLSKWEEAKKGIYKPYEIEAIGKDGCKHNLLITTTPVIGTDHYIIVQRD
                     ITEFKDLERKLYDSQKLAALGQLSAGIAHEIRNPLSSIKMSLQILTKRMKPDGNDLKR
                     FRIAEKEVEHLETLVNDILAFAKPVDPRKTSVDLSKVLEQAIALSEKGITDKKIEIVT
                     QFGKVFPVTVDAAMMTDAFINVIRNAVEALPENGKIVVSLKYADETRHSAAVEITDNG
                     SGIDETDMPHIFNPFFTRKNYGTGLGLSLVKKIIDIHRGTIDISSRKNEGTKVLIVLP
                     LRLDQAFSPAANNE"
     gene            6912..>7221
                     /locus_tag="PLG98_10220"
     CDS             6912..>7221
                     /locus_tag="PLG98_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOE33679.1"
                     /translation="MSHKLGKVILTAFTCMLVLLVASPFVFAAEAIPAGGESYIKVYF
                     AVGAMIGAGLAMGLGAIGAGAGIGNAANGACQAVGRNPGVQGKIMMVMLVGMAMAESI
                     A"
BASE COUNT         1906 a         1703 c         1533 g         2079 t
ORIGIN      
        1 tttcgacgag atagactttc attccggaat tggccaaatc gagagacgcc tgaatgccgg
       61 cgattcccga acccactacc attacagcac caactttttc gttatctttc aacacctgat
      121 tacacctcat ttattctgta ttaaagaact taaaaaactt ttttaatgcc gcgccgctag
      181 gcgttttttc gctaacacaa attcaataca ctgtcaagag cacaagctga atttatttga
      241 ttttttatgc taactcattg atataaaaag actaaaaaaa tgaccggcgt tcactgaccg
      301 gcattcaaaa ataaatagta tttcttggtc atgaatgttt gtcaagagcc cttaaatgat
      361 tttccagaaa ttcgaggatc tcaactttcc ctttttgtgt ctttgccgaa aacaaaattg
      421 tctttttttc acttgatatc cgcaacattt tttctatcgc gcgtttgcgc gctatcgcct
      481 ggttgttgga taatttgtcg gacttggtga gaatatataa ataaggaatg cggtaatgat
      541 ccagccagtc gcgcaacgat aaatcatcgg cattgggatc ccgccggata tccagaatga
      601 aaatcacgag acaaagagat tgcctctccc gaagataggc ctccatcata tccccccagt
      661 cttttttgac ggaccgggga actttggcaa aaccgtagcc gggcaaatcc acaaaagata
      721 ttgtttcgtt gacggtaaaa aaattgatta actgcgtccg tcccggcgtg ttactggttt
      781 tcgccagatt tttgcggccg acgagcgtgt tcatcagcga cgatttaccg acattggaac
      841 ggccgacaaa tgcgatctcc ggcatgattg ccggaggata ctgcggcggc cacacggcac
      901 tttttataaa ttgagcactg gtgattttca taattatctg acgcaatacc ttgtcttaat
      961 ctatgcgttc tattttctca ttgaccttaa gattctcttt tctttcaaga ctaaaaaagg
     1021 aaaggtcaac agcgttaaag cgtaatatat aacgattccc tttaaataac atccattttc
     1081 cccctgtcgt tccataatcg gcttgtaata tttcttaaaa aaatatgctg tgtaatacaa
     1141 cgaccagaaa agtttagcaa gagctccgac gcgttgccca ccggtttgaa cttccgaaga
     1201 gtgcatatgc acactgtgcg tatcattggt tataataatc tttccgccgt ttatgtgtat
     1261 tttcgcctga atgtcgtttt cctcacggaa tccgttgccc cggctgtaaa aagtatccat
     1321 ctcgtacgat aataaaagct ctcttctgac cagataaatg gcgtgggtaa aaggaagttc
     1381 tacatcgctt ttaaacttcg catcggtatt gatataaaaa aggcggaaat caaaaggcgg
     1441 cacgtcaata ctcccctgtc cgaaacgggc aatcgcttca gccacagtct cgcccggagt
     1501 gcggaaaaaa tggcgtcccg aaacaatatc cgccgacaga cttttcagtt tattaagaca
     1561 aactgtcgtg taattttctt ccagaaaatc gtcgtcatca ccgaaaagaa caaactcgcc
     1621 gtcagctgcc agaagtcccc gccggcgtga atagggagcg ccctgccttt tttcattttt
     1681 cagatacatt gtttttattc gaggatattt ccggacgaat tcatcaacca cgacagccgt
     1741 tttgtccgag cctccgtcat caacaatgat cagctctgca acaaactgct gccggtaata
     1801 cgaatccaga acctgagcaa gggtgtaccc ccggttgaag gtgggaatga taacgctgat
     1861 cataaaagtc tcagtaccct tcccgttatt tgtcatctca tttcatcaat aggtacaggt
     1921 caagactctg tatcctaaat acttacgaca ccgcacgttc attatttatt atcaaacaca
     1981 gttaaatctc gggagatcga tcatacgggt tttatctcta cttcgttcag aaaggaaatc
     2041 cggaggcgac cgatgagttt tatgggctgt ttaaaagcgc cgggatgcta atgctcaggt
     2101 atcgcggatt aagtcctgct tttgtcgcat gtcgctacga tatgagtaca gcgcaataaa
     2161 gcagaccacc gcgcccttta ggaggtatgc atttttcaac agcccatcat tcccgttcaa
     2221 aagtgatgcc gtatttttcc agtttccgtt ccagcgtcgg acgggagacc cccaggattt
     2281 cgcaaatctc acctttattc ttatccgtct ctttgataac tttgcgaata tagcgttctt
     2341 ccacttcgtc gagtgtaagc aacttgcccg gctcggacgc ttgaaacaaa ttcgcgtcac
     2401 ggtcaccggc cggcccggca gcttcttcct tgcccaattc cgggaaatcg ttgactccca
     2461 gcacctggcc cttggcgaca acgcaggcgc ggacaagcag attttccagt tcacggacat
     2521 tccccggcca ggtgtattgc ataaatattt ccatcacttc atcggaaaca ccgatgattt
     2581 tcttgtgaag atccagatta attttggtca gcaggtaatc gatcaggggc ggaatatcct
     2641 gacggcgggc gcgcagcggc ggcagcgtga tggaaacgat gttcaaccgg taatacaaat
     2701 catcgcggaa tttgccttcc ttaaccagcg ccttcaaatc cttgttggtc gcggccataa
     2761 tccgcgcgtt gaccttgact ttttcctttc ccccgactct gtcaaattcc atttcctgca
     2821 gaacacgcaa aagtttcgcc tgcaggttca cggacatctc gctgatctca tcgagaaaga
     2881 ccgaaccaaa acgggcgagt tcgaatttgc ccaatttgcg ggccacggcg ccggtaaacg
     2941 atcctttttc atgaccgaac aattccgatt ccagaagcgt ttcaacaata gcggaacagt
     3001 tcacggcaat aaaaggttcg tcgggcgacg tattgttatg aacgactttg gcgattaatt
     3061 cttttccggt cccgctttcc ccctgaatca gtacggtggt gcggctctgg gaaaccgtgc
     3121 cgatgatttt aaatatttcc cgcatctcgt tgccggtgcc gatgatatcg cccacacgaa
     3181 agctgcggga tggttccatc agaagcccgt ctatcttctt ttccatttcc agggatttca
     3241 gggctttttt aatcgccaga tccagttcgt cgacgttgac aggtttatga atataatcga
     3301 aagcgccgct tttcatcgcc tggatggttg tctccatgtc gtgaaaagcg gtaatcataa
     3361 tgacctttac attttcattc tcttccttaa ggtcttccag aacggtgaat ccgtcaatat
     3421 ccggcaggcg gatatccagg ataaccacat cggaaggttc ctgaacatat tgattcagac
     3481 cttccgtccc cgtcaacgcc gtgcggacgt tatatccttc ctccgtcaga tagagatcca
     3541 gggtctcggc aatggaagca tcgtcgtcaa tgacaagaat actcggcata atacagtcac
     3601 cttattcgtt attggccgca ggagaaaaag cctgatccag ccttaagggt aaaacgatca
     3661 gcacctttgt tccttcattt tttctgcttg aaatatcaat cgtcccgcga tgaatatcaa
     3721 ttattttctt taccagagaa agccccaaac cggtaccata attttttcgg gtaaagaaag
     3781 gattgaagat gtgcggcata tcggtttcat caataccgct gccgttatcc gtgatctcca
     3841 cagccgccga atggcgcgtt tcatcggcat actttaacga cacaacgatt ttgccgtttt
     3901 ccggcaacgc ttccacggca ttgcgaatca cgttgatgaa agcatccgtc atcatcgccg
     3961 catcgacagt caccgggaaa actttcccga actgcgtgac gatttcaatt tttttatcgg
     4021 taataccctt ttcggataac gcaatggctt gttccagaac cttggacaga tccacagacg
     4081 tttttcgggg gtccaccggt ttggcaaagg ccaagatatc attgaccagc gtctccagat
     4141 gctccacttc cttttccgca atcctgaatc gcttcaaatc atttccgtcc ggtttcattc
     4201 gtttcgtcag gatctgcaaa ctcattttaa tggaggaaag agggttgcgt atttcgtgcg
     4261 cgatgccggc ggaaagttgt cccagggccg cgagcttctg gctgtcgtaa agttttcttt
     4321 ccagatcctt gaattcggtt atgtcgcgct gaacaatgat ataatgatcg gtgccgatga
     4381 ccggcgtagt cgtaatcagg agattatgtt tgcagccgtc tttcccgatc gcttcgattt
     4441 catacggttt ataaatgcct tttttggcct cttcccattt ggacaaaacc agatcttcat
     4501 aaccggaagc aacaaagtcc aaaaaatatt tgtttttgaa ttcacccgat gtgcctgtct
     4561 tttttctcag gccccggctc ataaaattga tgatgccttt ttcatcgatt tcatagattc
     4621 tgtcaggcag actggtaatg atggtttgca gataattatt cagatcttga atctgtcgct
     4681 ggagttcaat attctcaatc aattcgttct gcagcgtttc ggcatattcg ctgaggctct
     4741 tcgtcatctc tctttcacgc gacacatcct gcaatgtttc aaccgctgca atgattttgc
     4801 cttcttcatc gtagatggga gccgccagaa aatacaggta acggctttgc ccgccgagat
     4861 tggcataata atccgtcgcc tcgtaggcgc ctacaacctt ttcggatttt ttgacgtttt
     4921 ttgttccgta atgtttgctg agcccgtcca tatcgctgtc gacgatcaaa tcggcaatca
     4981 tgggacgtgt cacggaataa aagggcttga agtgatttcc ggttcccagc atttcatcgg
     5041 cggaatggcc tgtcagttcg gaacaggcac gattccacaa gatgactttg tgcttattat
     5101 tgatgacaaa gctgggaatg ggcgatcctt cgatgatgcc ctcaatcgtc tttttttccc
     5161 gaagcagttt ttcctgccag tctcttcgct ccttgagccg ttttctttct tcccggtggc
     5221 gcagtctttt tccttcctta taggcaaaag acatgctggt cgctatcacg atgatgatga
     5281 gcagcaggga gctgaaaagg gtgtagaaaa aagtttttct caacggggag atgactttgc
     5341 tgtagggagc aacaaccagc atcgtccatt tcttatcgcc ggccttaacg ggataatagg
     5401 agatgatgct ccgttgtttt attttatcac cctggaggac aaccgcttca aagttatcgc
     5461 cgttctcgga tgaaaacatc gatgcctgtt cgtccgattt tttaagaaac ttttttatat
     5521 cgtttccgac aaaagcccaa cgaggatgaa ccagtatttt ttgctgttca tcgatcagcc
     5581 acaattctcc cagttcatcg tcggcgggtc gttgccggta tttatgtaaa actgcggaaa
     5641 gagaaaatga aatcagcaac gccccgctga attcgccatt tggcttccgg aggggatacc
     5701 ccaaaaccag atatctgtcg tgtttctgct ttgaaccgag cgtcccggaa gcatccggca
     5761 gagccaggcc cagataacgc ttttgctgtt tactgagcac attaaaatgc tcggatacat
     5821 tgatcgacgg cacaacgttt ttcggataaa cgtttttgat gtttccgccg gcatcaaaca
     5881 tgacaatcgc atcaaaagtg tcttcccacc cggaagacaa cattttataa taactatcca
     5941 gttccttttc atcaattgtc aaatcgggat ggaggcggga gaataaatca atatttttac
     6001 cgatctggga aaaaacatcc atcattctct tggaggtgtt ttgaacattg acaagctgct
     6061 gccggctgaa aatattgaca atgtcttttt cgcgggaatt gtttaccgtc agagccagaa
     6121 gcagaaaaag aatcacgacg gtaacactgg caaacaatat gcgtaattgt gcagctttat
     6181 aaagaaggat tttaaatttg ctgaataaag tcacctaatc tttttaatcc tctttgtttt
     6241 ctttatgaga aagctttatt ccgatcagga cataaaccag aattgccgcc gttaatgaaa
     6301 taagaagttt ggccaagccg ctttcaattc ttaaaacata cgaacagata agccatacga
     6361 tagggtaagt tattgccgtt aaaagctcct gcataatcat cctttataac gcgtctctta
     6421 attttatttt gacgatagta acggccttgg acgaaaattt caataaaaat ataagaaaga
     6481 acgtttcctt attcatcagt gcgctgtgac cactagcagc aagagaaaaa aatcaacccc
     6541 cgtatacgga agtattctgc cgaaagccac ttgaatgttt ttttatagcc gtatttaaca
     6601 aaacattaaa cgtcgcggca aaggaatgct tttaaaatga tcaaaaaaca ttttggatat
     6661 atttttcgga tatctttttt atgtcggtta agcgcgtcaa atgaaataat ctcaatacat
     6721 tagatatgcc tcttccttgt gacctccttt ttcatctttg aaaaatattt tcatatcaaa
     6781 gatatggcac aactcatgca gtaatcttgc cgcagcaaga ggtaaaatta tttcgctttc
     6841 catcgaatgt taatttatgt tatggaaaaa agaagtaata ataaacattt tattatgaaa
     6901 ggagacaaac aatgtcacac aaattgggta aagtaatttt aacagcattt acatgcatgt
     6961 tggttctgct ggtagcaagt ccattcgtat ttgcggcaga agctattccg gcaggtggag
     7021 aatcttacat taaagtgtat tttgctgtgg gcgcaatgat tggagccggt ctggcaatgg
     7081 gactgggcgc aatcggcgca ggcgcaggta tcggtaacgc tgcaaacggc gcatgtcagg
     7141 cagtaggaag aaaccccggt gttcagggca agatcatgat ggttatgctc gtcggtatgg
     7201 ccatggctga atccatcgcc a
//
LOCUS       DAOSME010000090         7104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_11695_length_7104_cov_19.066534, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000090 DAOSME010000000
VERSION     DAOSME010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11695_length_7104_cov_19.066534"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1467)
                     /locus_tag="PLG98_10225"
     CDS             complement(82..1467)
                     /locus_tag="PLG98_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33680.1"
                     /translation="MNLRQPHGSILINLIVITTIVAVLGAGMLYLTTTSTYASLFPNS
                     QARAYYLAEAGIAHAQQLLAQNIHNPSFYPGGHRGIDPPVTYTLANGDRFILTSYDFN
                     NGGTPDPTRVVVEAIGVVHAGTWMESKKKVTKSGILRDDPNPVDPTPSKKTEEFIDFM
                     VNDQVFVYGSAMSFSGNNVSGADATVVIRGPLDTDDTNLGASIAATTIYIDGHVELNK
                     GSAGLGSSSSPGNIYINGDLTLWNGSRSIYGDVYVNGNLRLKDAKIYGNIYVNGTVEL
                     GWTPTLSANANIYYVGDGNPDTPDIIHPKNYDQSILNKCHKIPYGAVQAFPSFSMPDV
                     AIPDPRGSAWFASKGYVSSGAPASGLKIYTEGDYNSSTWRPTANNVIIASKGNISITK
                     MGGSGLSGVLYAPYGKVTFEGGSFTGMVIARDGFFVTSGGTTVTFRGIENYIPNPDDY
                     PFKPDQQIIQY"
     gene            complement(1477..2013)
                     /locus_tag="PLG98_10230"
     CDS             complement(1477..2013)
                     /locus_tag="PLG98_10230"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE33681.1"
                     /translation="MMKLSKRGFTLIELIFTLVLAGIIVSAVGMGIVHVVQGFMFTKE
                     NAAALQKGQMALSRISLELKKAAGISDASTATSISFDTYADTGGPTPTLVTQMIRLNG
                     DKVEFVGSSGNPNILTDRAAAGNGLVFRYYTHYNDPEASQCDPKEARIVAITLRLAAA
                     DGVVKTFTARVRPRNLPY"
     gene            complement(2025..2459)
                     /locus_tag="PLG98_10235"
     CDS             complement(2025..2459)
                     /locus_tag="PLG98_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE33682.1"
                     /translation="MKRYLANRRGFTLLEIIVTLTILSILGVMVFQFMRSSLVRSTEP
                     FSIVAKTFVLSEVAENITADYLQNIPRNVPALKASIGAEGSEHINSGYCPAATDCRYS
                     VVDNHYIAFDGNHEVPTTVQGLLKVTIRGEQNETITMVFIQP"
     gene            complement(2456..2977)
                     /locus_tag="PLG98_10240"
     CDS             complement(2456..2977)
                     /locus_tag="PLG98_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE33683.1"
                     /translation="MNLLPPEGRMEETEMKKRASRNDRENGFSMFEVIVVILLIGVVA
                     AFVAVRGSSKTVYDLASEAEILKGHLRYAQFRAMSDTESWGMSLSTNGYQLLKNKAPA
                     TDSLPNESGPSHALPSGITITTGAGTAIHFNERGIPVGDGDAPLTAETEIALSHGSAT
                     QTIFIKPETGFIP"
     gene            3353..3874
                     /locus_tag="PLG98_10245"
     CDS             3353..3874
                     /locus_tag="PLG98_10245"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE33684.1"
                     /translation="MDEGTLMNRICRKNNKNRQHGFTLLEVIAVIVIVSVLAAVAVSR
                     FASTSSTSLVAETDILKAHLRYAQYRAMSDAMPWRLSFSTTTYSLSYYDESTSQWKSS
                     TLPNEASGTHSLEKAGVTLTVAGGDGSSIYFNEWGNPVNASNNLLSSPQIVLTAGGKT
                     QTISITTNTGFIP"
     gene            3871..4281
                     /locus_tag="PLG98_10250"
     CDS             3871..4281
                     /locus_tag="PLG98_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE33685.1"
                     /translation="MNVNKENEKGFTLIEVIVTIVVLSILSVFAYQYMGSSLSGSSEP
                     IARLKQSLSLQQVAENITSDYKKNYSTNLPDFKTKIENPSASGYGTYTVVESKYVKFT
                     GSQETNADPNVYNMLKITIKNSQNETITMLFVGL"
     gene            4300..4821
                     /locus_tag="PLG98_10255"
     CDS             4300..4821
                     /locus_tag="PLG98_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE33686.1"
                     /translation="MKHSKKGFTIIEVVVSLVLIGIMALVLGLGFYNMVRGYLFARDN
                     EVITQKGQIAMSRIAQELKNITLVSSPSTPTSITFYSYKNDVKSKRSIRLSDDGKEIQ
                     ITESYSEEAPVYYPLTDQLAPNGFTLEYYANYNDTTQCYPPSARIIIVKLRLAGPDNY
                     ILELIDRVAPRNI"
     gene            4837..6240
                     /locus_tag="PLG98_10260"
     CDS             4837..6240
                     /locus_tag="PLG98_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33687.1"
                     /translation="MNNPDFRYNIAAYVINGIKSFSKRGFMPQRGSVLISIIVGTVII
                     GILGVGIISFTTTSTYGELFANRQVRAYYMAEAGGQYAIKLLNENKSNTSYLPPNGTY
                     KLGPNGSDGEFSLTSTSDNTDPDMPRVKVVSVGVVHKGTWLESKKKITYKIPRANPTA
                     GLDGTTDISFDGNNNDVLDPVWVPTVSQDGGTVEYDKISDAVLIKTKEALIALSPTAI
                     DFITARTNSGGLLSYEVQVKINMNFKDDKADYIAMGLNFRLDKALKRSYGISFYRAIT
                     KENQMPAWFPERFKPSFTSTPPRGVNYYVVLWVQNGTDKYTLIDYALLDNSIYGGFET
                     KQYNTILLNLREECDTPPGSATGCTTTRHNLIQVYLADSSSTSLYPKGTINWSYATNT
                     SVFKRVKWVLSNSETINDSTLTTQYWNSTWQPYSSSVPSEVGLHALYYIRPSQEEFFA
                     DFSMRGEGLGGSGGYQY"
     gene            6368..6991
                     /locus_tag="PLG98_10265"
     CDS             6368..6991
                     /locus_tag="PLG98_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33688.1"
                     /translation="MGKFSVFVFALMIIIGIGLALHRGNDDADKSGKQAPAGVAEKES
                     TGEKISIEELASKGEKVSFDEALSKIDQANAEIKEQAEKFSIRKMIERVQKDGPAGLV
                     AHFRGENGESHDPKSQPAALAELSGKGAQVQRAEKDNTGSVRLEIERLRKEEDARRRQ
                     QDKATAQKGQAPQQSIQEKPGENEKKDFLSELREMWETFKSVLASSE"
BASE COUNT         1887 a         1673 c         1796 g         1748 t
ORIGIN      
        1 acaggctcag catgacaaaa gagggacaag atgacttttt acgaaaccgt caaaattgac
       61 tttttacgaa gccgtcatct ttcagtactg gatgatctgc tgatccggtt taaaggggta
      121 atcatcgggg tttggtatgt aattttcgat tccccggaaa gtgacggttg tcccgccgct
      181 ggtgacgaaa aagccgtccc gggcgatgac catgcccgtg aaggagccgc cttcgaaggt
      241 gacctttcca taaggggcgt acaaaacgcc ggacaagccg cttcctccca tctttgtgat
      301 gctgatattg cccttgcttg cgataattac attatttgcc gtaggccgcc aggtcgatga
      361 gttgtaatcg ccttcggtat atattttcaa accgcttgcc ggcgcgccgg acgacacata
      421 gcctttggat gcaaaccagg cgctgccgcg cggatccggg atggctacgt ccggcatgga
      481 gaaggacgga aaagcctgca cagctccata gggtattttg tgacatttgt tcagaatgga
      541 ttgatcgtaa tttttgggat gaatgatgtc gggcgtatcg ggattgccgt ctcctacata
      601 gtagatgttg gcatttgcgg aaagggtggg ggtccagccc agttcgaccg ttccgttgac
      661 ataaatgttt ccgtagattt tcgcatcctt caggcgaaga ttcccattca cgtaaacatc
      721 tccatagatg ctgcgtgatc cgttccacag ggttaaatcg ccgttgatat aaatatttcc
      781 gggcgaagaa ctggatccga gccctgcgct gcccttgttc aattctacat gtccgtctat
      841 gtaaatggtc gtagctgcaa tagaagcgcc caggttggta tcatccgtat ccaagggtcc
      901 gcgaatcaca acggttgcat cggcgcccga tacgttattt ccactgaagc tcatggcgct
      961 gccgtaaacg aacacctgat cgttgaccat gaaatcgatg aactcttcgg ttttcttgct
     1021 gggcgtcggg tcaacgggat tggggtcgtc acgaaggatg ccgctcttgg tcactttctt
     1081 cttggattcc atccaggtgc cggcatgtac gacaccgatg gcttcgacga caacgcgtgt
     1141 cggatcgggg gttcctccgt tgttgaagtc atagctggtc aggatgaacc ggtcgccgtt
     1201 ggccaaagtg taggtgaccg gcgggtcaat gcctctgtgg cctccgggat aaaaggaagg
     1261 attgtggatg ttttgagcca gaagctgttg cgcgtgggca atgcccgctt cggccaggta
     1321 gtaagcgcgg gcctggctgt tcgggaaaag actggcgtat gttgaagtcg tcgtcagata
     1381 cagcattccc gcgcccagaa ccgcaacgat tgtggtgatg acaatcaaat taattaaaat
     1441 ggaaccgtga ggttgacgga gattcataca gactcctcaa tacggcaaat ttctgggccg
     1501 aacgcgagcc gtgaaagttt tgacgacgcc gtccgctgcg gcaagacgca acgtaatggc
     1561 gactattctg gcttccttcg ggtcacactg acttgcttcc ggatcgttgt aatgggtgta
     1621 gtaccgaaag accaggccgt tgccggcagc cgcccgatct gtcaagatat tgggatttcc
     1681 tgaggagccg acaaattcca ctttgtcgcc gtttaaccgg atcatctgcg tcacgagggt
     1741 cggtgtcggt ccgcccgtgt ccgcataggt atcgaaagaa atcgatgtcg ccgtcgaggc
     1801 gtccgaaatg ccggcggctt ttttcaactc cagtgaaata cggctgaggg ccatctgtcc
     1861 cttctgtaga gcggcggcgt tctctttcgt gaacatgaat ccctgaacga cgtgaacgat
     1921 gcccattcca acggccgaaa caatgattcc cgccaaaacc agtgtgaaga tcaattcgat
     1981 cagcgtaaat cctcttttgg ataatttcat cattcgacat tccttcatgg ctggataaaa
     2041 accatggtga tcgtttcatt ttgttcgcca cggatggtga cctttaataa tccctgaaca
     2101 gtggttggaa cttcatgatt cccatcaaag gcgatgtagt gattgtcgac aaccgaatat
     2161 cggcaatccg tcgcggcggg acaatatccg ctattgatgt gttccgatcc ttccgctccg
     2221 atgctggctt tcagcgcggg tacattccga gggatgtttt gcaggtagtc cgccgtgata
     2281 ttctcagcca cttcgctcag gacaaatgtt ttggcaacga tggaaaaggg ttccgtgctc
     2341 ctgacaagag aagacctcat gaattgaaac accatgacgc ccagaatgga caggatggtc
     2401 aacgtgacga tgatttccag gagggtgaat cctctgcggt tggccaggta acgtttcatg
     2461 gtatgaaccc cgtttcgggt tttatgaaga tggtctgtgt ggcggatcca tgggacaggg
     2521 caatttcggt ttctgcggtc agcggcgcgt cgccgtcgcc caccggaatt ccccgttcgt
     2581 tgaaatgaat agccgttcct gcgccggtcg tgatggtgat tcctgaaggc aaagcatggg
     2641 aggggccgct ttcattgggc agcgagtccg tggcaggcgc cttgtttttc agcagttgat
     2701 aaccatttgt ggagaggctc attccccagg attcggtatc gctcatcgcc ctgaattgag
     2761 cgtaacgcag atgccccttg aggatctccg cttccgacgc cagatcgtaa accgtcttcg
     2821 atgatccgcg gacagcaaca aaggcagcga cgacgccgat gagcaaaatg acaacgatca
     2881 cttcgaacat cgaaaaaccg ttttccctgt cattccgcga agctctcttt ttcatctccg
     2941 tctcttccat ccggccttcc ggcggtaaca aattcacgac atcaatcgag ccgccttaaa
     3001 atggttactg ccgatcctat ggaagaaaag aagatatgtc aagaagacta acaatctgct
     3061 ttttataaca gaatcttcat ctctcttcat aacagaaaat ccggctgcaa gacatccgga
     3121 cgcatacgcc cgatgcattt gctgcctctt cccggttcac gcctctttct gcttgccgtt
     3181 atgaaattac caaatatatg atatgataca taaaatcgat aacaggcaag ccggggatgc
     3241 atttggttgg ccaacggaaa gaacgacagt tatactttga attcattatt gactatgcta
     3301 aaatgtagac gcaatctcga aagctcaacg cttgcggact ttgtagcttg ttgtggatga
     3361 aggaacgtta atgaacagga tttgccgtaa aaacaataaa aaccgtcaac acggtttcac
     3421 attgctcgaa gtcatcgccg tcatcgttat tgtttccgtt ctagctgccg ttgccgtgtc
     3481 acggtttgct tccaccagca gtacatcgct tgtagctgaa actgatattc tgaaagccca
     3541 tctgcgttac gcgcaataca gggccatgag cgatgcgatg ccatggcgtt tgagcttttc
     3601 gacgactacc tattcgcttt cgtattacga tgaatcgacg tcgcagtgga agtcttcgac
     3661 gctgccgaat gaggcgtctg gaacgcacag tctcgagaaa gcgggtgtga cattgacggt
     3721 cgcaggcggg gacggttcgt ctatatattt taatgaatgg gggaatccag tcaatgcctc
     3781 taacaatttg ctcagtagcc cccaaatcgt tttgacggct ggcgggaaga cgcaaacaat
     3841 ttcgataacg acgaatacag gcttcatccc atgaacgtaa acaaggaaaa tgaaaaaggc
     3901 ttcaccttga tcgaggtcat tgtcacgatt gttgtcctct cgatcttgag tgttttcgcc
     3961 tatcagtaca tgggatcctc tctatcgggc agttctgaac cgattgcgcg cctgaaacaa
     4021 tcgctgagcc ttcagcaggt tgctgaaaat atcacatctg actacaagaa gaattattca
     4081 acaaatcttc cggactttaa aaccaagatt gaaaatccct cggcaagcgg atacggaact
     4141 tacacggttg tcgaaagcaa atacgtaaaa tttactggca gccaggaaac aaatgccgat
     4201 ccaaacgttt acaatatgtt aaaaatcacc attaagaata gccagaacga aaccatcaca
     4261 atgcttttcg taggactgta aaaagtatca ggtgttcgaa tgaaacattc aaaaaaaggt
     4321 tttacgataa ttgaggttgt tgtttcactg gtgttaatcg gcattatggc tcttgtttta
     4381 ggactgggct tttataatat ggttcggggc taccttttcg cgcgcgacaa tgaggtaatt
     4441 acgcagaagg gacagatcgc catgagccga attgcccagg aactcaaaaa catcaccctg
     4501 gtttccagtc cgtcaacgcc cacgtccatc acgttttact cttataaaaa tgatgtcaaa
     4561 agcaaacgat ctattcgcct cagtgacgac ggtaaggaga ttcaaattac agaaagctac
     4621 tcggaagagg cgccggtata ttatcccttg acggatcagt tggctccgaa tggttttaca
     4681 ctggaatatt atgcaaatta caatgataca acgcaatgtt atccgcccag cgcaagaatt
     4741 attattgtga aattgcgcct tgccggtccg gataattaca ttctggaatt aatcgacagg
     4801 gtcgctccaa gaaatattta gaatgggcaa aatataatga ataacccaga tttcaggtac
     4861 aacatagcgg catacgtgat caatggtata aaaagcttct ccaaacgagg atttatgcct
     4921 caacgcggtt cggtgctcat cagcatcatt gtcggtacgg taattatagg gatccttggt
     4981 gtagggatca tctcctttac aaccacctca acgtatggag agctttttgc gaatcgccag
     5041 gttcgggcat attacatggc tgaagcggga ggacaatacg caataaagct gcttaatgaa
     5101 aacaaatcca ataccagtta cttgccgcct aatggcactt acaaattagg gccgaacggt
     5161 agcgatggcg aattctcact caccagcacc agcgacaaca cagacccgga tatgccgagg
     5221 gtgaaagtgg tatcggtagg tgttgtgcat aaagggacat ggctggagtc gaagaagaag
     5281 attacttata aaattccgcg agccaatccc acggcgggac tcgatggaac aacggacatc
     5341 agttttgatg gaaataacaa cgatgtcttg gacccggtgt gggtaccgac ggtcagtcag
     5401 gatggaggta cggtggaata cgacaagatt agcgatgccg tcctgattaa aacaaaagag
     5461 gctttgatcg cactgagtcc gaccgccatt gatttcataa cagcgcggac gaattctggg
     5521 ggattgttga gctatgaagt ccaggtcaag atcaacatga atttcaaaga cgataaagcc
     5581 gattatatcg ccatgggcct caatttccgc ttggacaagg cattgaaacg ttcctacgga
     5641 atatcctttt acagggcgat tacgaaggag aatcaaatgc ccgcttggtt tcctgaacga
     5701 ttcaagccga gtttcacttc aactcccccg cgcggcgtaa attattatgt tgtgctttgg
     5761 gtgcaaaacg gcactgataa atacactctg attgattatg cgctgcttga caacagcatt
     5821 tatggtggct ttgaaacgaa gcagtacaat acgatactgc tcaatcttcg cgaagaatgc
     5881 gatacccctc cgggcagcgc gaccggatgc acaacaaccc gccataatct cattcaggtg
     5941 tatctggctg actcatccag tacatcacta tatcctaaag gaactatcaa ttggagctac
     6001 gcgaccaata catccgtttt caaacgggtg aagtgggtac tgtcaaattc ggagacgatc
     6061 aacgactcaa ccctcaccac tcaatattgg aattctacgt ggcagcctta tagttcctct
     6121 gtcccatcgg aggtcggcct gcatgcttta tattacattc gacccagcca ggaagaattt
     6181 ttcgccgact tttccatgag aggagaggga ttgggcggaa gcggtggtta ccaatattga
     6241 cgacgtgttt tttttagaac gaatgcgaag agcagtactc ctcaagaaca gaaagttacc
     6301 acctttgtga tgccgaccgc gataatttca atcatgcctt aaaattcttt caccttaggg
     6361 aattattatg gggaaattca gcgtctttgt ttttgctttg atgattatta tcggcatcgg
     6421 tttggcctta cacagaggaa acgatgacgc ggataaaagc ggaaaacagg ctcccgccgg
     6481 tgtcgctgaa aaagaatcaa ccggggaaaa gatatccatt gaagaattag cttccaaggg
     6541 agagaaagta tcgtttgatg aggctctgtc aaaaattgat caggccaacg cggaaataaa
     6601 agagcaggcg gaaaaattca gcatccgcaa aatgatcgag cgtgtgcaaa aggatggacc
     6661 ggcaggtctg gtcgctcatt ttcgcggaga gaacggcgaa agtcatgacc cgaaaagtca
     6721 accggccgcg cttgcggagc tttcagggaa gggggctcag gttcaacggg cggaaaagga
     6781 caacaccggt tccgtccggc tggaaataga gcggctgcgc aaagaagaag acgccagacg
     6841 ccggcaacag gataaggcga cagcgcagaa aggccaggcg ccgcagcaga gcattcagga
     6901 aaaaccaggc gagaatgaaa agaaagattt tctttcggaa cttcgggaga tgtgggagac
     6961 cttcaaatcc gtcctcgctt catcggaatg agatttacgg taaacggcaa attccgccat
     7021 ggcgccgcgg ccaagaataa ctgacagaaa cgtttgcata accacataaa aacaacaata
     7081 ttgtcattcc cgcgaaggcg ggaa
//
LOCUS       DAOSME010000091         6953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_12008_length_6953_cov_21.127573, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000091 DAOSME010000000
VERSION     DAOSME010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12008_length_6953_cov_21.127573"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..667
                     /locus_tag="PLG98_10270"
     CDS             50..667
                     /locus_tag="PLG98_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33689.1"
                     /translation="MNFLSFKNALKGFVSFKLNDIRKIDPGFDLRRLSEWQDKGFIKM
                     TRRGHYIFSDLELNESVLFLIANNIYSPSYISMEMALAYYNLIPEAVYGITSVSSCKT
                     NRFKTDFGEFIYRHVKPQLIFGYILVGHEGQNFKIAEPEKAILDYFYLNAHLDTKEDF
                     AGLRVNSEEFKEQMDRDKLKRYLVAFGNKRLEKRFNKFLKCINHA"
     gene            726..1436
                     /locus_tag="PLG98_10275"
     CDS             726..1436
                     /locus_tag="PLG98_10275"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE33690.1"
                     /translation="MREYLQYKILEIVFNSVLGTKLSFLGGTALRIAYENTRFSEDLD
                     FDNFNLSETEFVILTKEIQRGLQREGFNVEIRNVIKRAFRCYIKLPQVLFDNAMSDLK
                     NEKILIQVDTEPHNFSYAPDKLILNKFDIFTQIAVTPPDVLLAQKIYALLNRKRAKGR
                     DFYDTVFLLQKTKPNYDYLKQKIGIADAQSLKEKILDYTKDLNLKVLARDVESFLFNP
                     SDSKKIVLFPDYISQVEL"
     gene            1951..2721
                     /locus_tag="PLG98_10280"
     CDS             1951..2721
                     /locus_tag="PLG98_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOE33691.1"
                     /translation="MEQVYDIFKSFVDPVFIIFILLLIMFFIYLASSKKKIDMLPLLF
                     VIILLYGFSIFPVSNYLSYQLEKEYISPAAVKDKMTPDVIVVLSGGASDINALDETFP
                     GETTTVRLVHAVQMFKKYNARYLVCSGKSDGKVADARIMAKMAEELGVPRDKIRIEPK
                     SRNTFEHAEEFKKMFADKNLKVGLVTSASHMKRSQRQFRKYFNNVLALPTGYLYASPA
                     GTAAVRYIPHSRWLYNNALVFHEFVGQMWYSIKTVNSE"
     gene            3060..4958
                     /locus_tag="PLG98_10285"
     CDS             3060..4958
                     /locus_tag="PLG98_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOE33692.1"
                     /translation="MYAQFKNPNFYLMVITDVLLFTIALYLSYFFRFDFNFANINFEQ
                     IGDLLFWILPLKVFIFFTIGLYRGMWRYTSVKDFWLLIQACFIATVLIMVIILTISRF
                     SGYSRSIFFIDGIFTFVLTGGVRMAIRSFFAMFGNQAMNVSSSLLSKTTRVLIVGAGD
                     AGEKILREINDNYKLNYEVVGLIDDDEQKQGRTIHGVRVLGPVDRLPKILKRETIHQI
                     LIAVPSASGDRIRRIVETCQQCNLDYKILPGIGDLIDGRVSVKLLRDISYEDLLGRSP
                     VQLNVRDIRNYLDDKTILITGCGGSIGSELCRQVVKYQPHRLILLDSSESNLFNIQME
                     IINEHFFRYCEAILGHVQDEALMNNIFEKHKPEVVFHAAAYKHVPMMEKNPWQAVLNN
                     IMGSRVAMEMAIKHHVERFVLVSTDKAVRPTNVMGASKRVTELIMQCQQGNGTRFMAV
                     RFGNVIGSSGSVIPLFRRQIEQGGPVTVTHPEINRFFMTIPEAAQMILQAGTMGEGGE
                     IFILRMGTPIKIAEMARDLIRLSGKEPDVDIKIVFTGLREGEKLYEELITVGEDIMPT
                     SHKKVMVLQSNGFINGAKNSEEAKIYLYNKLDQLAGLAAQHDARGIKIKLKEIVPEYT
                     PQENESVF"
     gene            4996..5370
                     /locus_tag="PLG98_10290"
     CDS             4996..5370
                     /locus_tag="PLG98_10290"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOE33693.1"
                     /translation="MVKRAHKNLDVWKESVSLATHIYKVTEKFPQNELYGLTSQMRRA
                     VISISSNIAEGAARNSAKEFSHFLNIAGGSLSELDTQIEIAHNLLFVTSEQKQDMNIM
                     IESISGKLAGLIKYVKKKSHEK"
     gene            5561..>6953
                     /locus_tag="PLG98_10295"
     CDS             5561..>6953
                     /locus_tag="PLG98_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOE33694.1"
                     /translation="MKTIIAIFLVLVLKTPGYALSSTNIWIGNALYADVESWAAEGLI
                     ESQLSSMKPMARSELGRQLVTALDRCNTSQAPSINCVNIQQYYKKVYDAELSEARNAS
                     SVSGSFIKPVENVSFSFNYLDGPFSIYNNEGIDFGEGSNALVQLQSSARLWKAFSFFV
                     QPVLIYNHHAYTSGDDSETKLRLHRGYAKVNLFNFEIQAGRDSLWWGPGYHGSLLLSN
                     NAKPFDMIKLSNPEPVLLPWFFSYLGPFQFNLIFSQLHDERTGIELANPFLYGLRFDF
                     KPHPYLEVGLSHLSMFGGPGRRDLSIGDVLKVLYSNTNPDPSEKLDSNAQVALDLALT
                     LPNVKKYIFIADALKLYTEWGAEDTGFPPDKRAYIGGLALFKPFGLERAVLRGEYARM
                     SPGSLPGAWYSHPTYPMRYNGRVFGHHAGNDSDDIFVEWSQTFDKFSYKLSFDRERSG
                     IGTKSFVQTKNQYL"
BASE COUNT         1940 a         1464 c         1623 g         1926 t
ORIGIN      
        1 attataattc cgtaatatgt taataatcac ataatatgga aatataagta tgaatttcct
       61 gtcctttaaa aatgctttga aaggttttgt ttcgtttaaa ttaaacgata ttcgaaaaat
      121 cgatcccggg tttgatctcc ggcgcctgag cgaatggcag gataaaggct ttattaaaat
      181 gaccagaaga ggacattata tcttttctga tcttgagcta aacgaatccg tccttttttt
      241 gatagccaat aacatttatt caccgtccta tatatcaatg gaaatggcgc ttgcatatta
      301 taatctgatt ccggaggcag tttatggtat tacgtcggtt tcgtcctgta aaacaaatcg
      361 tttcaaaaca gatttcggtg aatttattta tcgtcatgtc aaaccgcagt taatattcgg
      421 ttacatatta gtcggtcacg aaggccaaaa ctttaaaatt gcagaaccgg aaaaagccat
      481 tctggattat ttttatctca acgcgcatct ggatacaaaa gaagattttg ccggcttgcg
      541 tgtcaatagc gaagaattca aagaacagat ggatcgggat aaactcaaaa gatatcttgt
      601 tgccttcggc aataaacggt tagaaaaaag attcaacaaa tttttaaagt gcataaacca
      661 tgcttagcat caatcagatc gaaaaatatt atcccgaaag tctgcgcggc tttaaacgaa
      721 acattttgcg tgaatatctg caatacaaaa ttttagaaat cgttttcaat tccgtcttgg
      781 gtacaaagct ttcatttctc ggtggcacgg cattgcgtat cgcttacgaa aacacacgat
      841 tttcagaaga tctcgatttc gataatttta acctaagcga aaccgaattt gttattttga
      901 ccaaagaaat ccaacgcggg cttcaaaggg aaggtttcaa tgtggaaatt cgtaacgtca
      961 taaagagagc tttccgctgt tacatcaagt tgccgcaggt tttgtttgat aacgccatgt
     1021 cggatttgaa gaacgaaaaa atcttaattc aggttgatac ggaaccgcat aatttttcct
     1081 acgcaccgga caagctgatt cttaataaat ttgatatttt tacacagatt gcagtcacac
     1141 ctcctgatgt tctgcttgca cagaagattt atgcgctgtt gaatcgcaaa agagcaaaag
     1201 gtcgtgattt ttatgatacg gtgttcttgt tgcaaaaaac aaagcctaat tatgattacc
     1261 tgaagcaaaa aatcggcata gccgatgctc aatctctcaa agaaaagatt cttgattata
     1321 caaaagatct taatctgaaa gtgctggcaa gggatgtgga gtcattttta ttcaatcctt
     1381 ctgatagtaa aaaaattgtt ttatttccgg actatattag tcaggttgaa ctttaaaaaa
     1441 ggcgtgaaag gcggcagtcg gcggtttgct attggtgata agcgtaaaat tttctcttga
     1501 taaaagtgaa caattattgc gttatttgtt cacttcatta gacaataatg aaagctttgc
     1561 ataaccaagt ccatttattt attctgtcat accggcgtag gccggtatcc agttatttca
     1621 ggtacttcct ggattccggt tttcaccgga atgacgaaaa aaagaattat gcaaagctct
     1681 caataatata gggcaacaga aattgctaaa taaatcaaaa ctgaacggtg cactattacc
     1741 tcggcaatta aagattgtaa taacgggcaa ttttattttt actttaatgc agcttctttt
     1801 ttattactgg attcccgccg gatgtttacc ccgcgtatgc ggagcgcggg aataacgcca
     1861 tttatgctat gttgccggcg aaataatagt cttgtaaaat gaaagatcgt attttaatat
     1921 gcaacagtaa attaaaggat aaaacaatat atggaacaag tttacgatat atttaaatca
     1981 tttgttgatc cggtttttat cattttcatt ctgttgctga taatgttttt tatttatctg
     2041 gcctcaagca aaaagaagat cgacatgctg cctttgcttt ttgttattat tttgctctat
     2101 ggtttcagca tctttcccgt gtccaattat ttaagttacc agcttgaaaa ggaatacatc
     2161 agcccagcgg ctgtgaaaga taaaatgaca ccggatgtca ttgtggttct cagcggcggc
     2221 gcttccgata tcaacgcttt ggatgaaaca tttccgggag aaacgacgac tgtcaggctg
     2281 gtgcatgcgg tgcagatgtt taaaaaatac aatgcccggt atctggtctg ttccgggaaa
     2341 agcgacggta aggtagccga cgcgcggatt atggcgaaaa tggctgagga attgggtgtg
     2401 cccagggata aaatccgtat cgagccgaaa tcaagaaata cttttgaaca cgcggaggaa
     2461 ttcaagaaaa tgtttgccga caaaaatctc aaggtcggat tggtcacctc cgccagccat
     2521 atgaaacgaa gccaaagaca gttccggaaa tattttaaca atgttcttgc tttgccgaca
     2581 ggttatcttt atgcttcgcc cgccggtacg gccgctgtcc gctatattcc tcattcccgc
     2641 tggctttaca acaatgcgct tgtttttcat gagtttgtcg ggcaaatgtg gtacagtata
     2701 aagacggtga atagtgaata gtgaatggta atgacattcg tcgagaacaa acaaagtgca
     2761 gttcgagacg taaagaaagc tttgcataac cagagccatt tacttattct gtcataccgg
     2821 tgttttccgt accgctgtca ttccggcgtg ggcctggccg gaatccagaa atattaaaac
     2881 actggttccc ggctttcgcc gggaagacgg accgggggga ttccggtttt caccggaatg
     2941 acgaaaaaaa aattatgcaa agctttcgta aatggtagtg agactcgcta aagatatgaa
     3001 gcgttagtcg aactatccaa tcccgataaa tagggattga tggttgatgg tgaataggga
     3061 tgtatgctca gtttaaaaat ccgaatttct atctgatggt cattacggat gtcctgctgt
     3121 ttaccattgc gctttatctg tcgtattttt tccggtttga ttttaatttt gccaatatta
     3181 atttcgagca gatcggcgac ctgcttttct ggattcttcc gctcaaggtt tttatctttt
     3241 ttacgatcgg gctttaccgg ggaatgtggc gctacaccag cgtcaaggat ttctggcttc
     3301 tgattcaggc ctgtttcata gcgaccgtgc tcattatggt tattattttg accatcagcc
     3361 ggttctccgg gtattcccgc agcatttttt ttattgacgg tatttttacg tttgttttaa
     3421 ccggcggcgt acgcatggcc atccggtctt tttttgccat gttcggaaat caggccatga
     3481 acgtcagctc gtcattgctc agcaaaacca ccagagtcct gattgtcggc gcaggtgatg
     3541 cgggcgaaaa aattttacgc gaaatcaatg ataactataa attgaattat gaagtggtcg
     3601 gtttgatcga tgacgacgag cagaaacagg gccggacaat tcacggggtc agagtcctcg
     3661 ggccggttga ccgcctgccg aaaattttga agcgggaaac gattcatcag atcctcattg
     3721 cggtgccttc cgccagcggc gatcggatac ggcggattgt agaaacctgc cagcaatgta
     3781 atttggacta taaaatactg ccgggtatcg gcgacctgat tgacggccgc gtcagcgtga
     3841 agctgctccg ggatatcagc tatgaggatc ttctgggacg ctcgccggtg cagcttaatg
     3901 tgcgggatat ccggaattat ctcgatgaca aaaccattct gatcaccggg tgcggcggct
     3961 ccattggttc ggagctttgc cggcaggtcg tcaaatacca gccgcaccgt ctgattctgc
     4021 tggattccag cgaatctaat ctttttaata ttcaaatgga aattataaac gaacatttct
     4081 tccgatactg cgaagccatc ctggggcacg tgcaggatga ggcgttaatg aataatattt
     4141 ttgaaaaaca caaaccggaa gtcgtgttcc acgcggcggc ctacaaacat gtgcccatga
     4201 tggagaaaaa tccgtggcag gcggtattaa ataacatcat gggaagccgg gtggcgatgg
     4261 agatggccat caaacatcat gtggaacggt ttgttctcgt ttccaccgac aaggccgtgc
     4321 gtccgaccaa tgtcatgggc gccagcaagc gcgtgacgga attgatcatg cagtgccagc
     4381 agggcaacgg cacgcggttt atggccgtgc ggttcggcaa tgtgatcggg tcgtccggtt
     4441 ccgttattcc gttgttccgc cgccagatcg aacagggcgg accggtcacg gtcacgcatc
     4501 cggaaatcaa ccgtttcttt atgaccatac ccgaagcggc acagatgatt ttgcaggcgg
     4561 gcacgatggg cgaaggcggc gagattttta ttctgcgcat gggaacgccc ataaaaattg
     4621 ccgagatggc gcgcgacctg atccgccttt ccggcaagga accggatgtg gatattaaaa
     4681 ttgtctttac cggtttgcgc gaaggcgaaa aactttatga agagctcatc acggtcggag
     4741 aggatataat gcctaccagc cacaaaaaag tcatggttct tcaatccaac gggtttatca
     4801 acggcgcgaa aaattccgaa gaggccaaaa tatatctcta caataagctg gatcagcttg
     4861 ccggactggc ggcgcaacac gatgccagag gcattaaaat aaaactcaag gagattgtac
     4921 ccgaatatac cccgcaggaa aacgagagtg tcttttgaat gtagtgaaga gtgaaaagtg
     4981 aagggtgaag agtgaatggt aaaaagagca cacaagaact tggatgtctg gaaagaaagc
     5041 gtttcacttg ctactcatat ttacaaagtt acagaaaaat ttccccaaaa tgaactttac
     5101 ggattgactt cgcaaatgcg ccgtgcagtt atttctattt caagtaatat tgcagaagga
     5161 gcggcaagaa attcggcgaa agaattttct cactttctga acatagctgg cggctcactg
     5221 agcgaattgg atacacaaat tgaaatagcc cataatcttc tttttgttac cagtgaacaa
     5281 aagcaagata tgaatattat gatagaatcc atatccggca aattggcggg attaattaaa
     5341 tacgtgaaga aaaaatcaca tgaaaaataa ctttttagta agaccagttg agaacgagtg
     5401 caacgaaggt cgaaacgtaa atcgtagtga cacccgcggc gaacaagcca aacaccgttc
     5461 aaagcgtcgg tggaactatc ccgggggtga atactctaaa gggtactata ggcgccgtgg
     5521 gggaccattc accgttcacc attcactatt cactttctta ttgaaaacaa taatagcaat
     5581 tttccttgtc ctggttttaa aaacgccggg gtatgcgctg tcgtcgacaa atatctggat
     5641 cggcaacgcg ctgtatgccg atgtggaatc atgggcggcg gaaggcctga ttgaaagcca
     5701 gttatcttcg atgaagccga tggcccgcag cgaacttggc aggcaactcg tgacagcgct
     5761 ggataggtgc aatacatcgc aagcgccttc cattaattgt gtcaatattc aacagtatta
     5821 taagaaagtt tatgacgcgg aactcagcga agcaaggaac gccagtagcg tatccggcag
     5881 tttcatcaaa ccggtggaga atgtttcttt ttccttcaat tacctcgacg gccccttttc
     5941 catttataat aacgagggaa tcgatttcgg cgaaggaagc aatgcgctgg ttcaactcca
     6001 gtcgtcagcg aggctctgga aggcgttttc ctttttcgtg cagccggtat tgatttacaa
     6061 ccatcacgcc tatacctcgg gggatgacag cgaaaccaaa cttcgcctgc accggggtta
     6121 cgcgaaagtg aacctcttca attttgaaat ccaggccgga cgcgattcgc tctggtgggg
     6181 accgggttac cacggctcgc ttttgttgtc caacaacgct aaaccttttg acatgatcaa
     6241 attgtccaat ccggaaccgg tgctcctgcc gtggtttttt tcatacctcg gcccctttca
     6301 gttcaacctg attttttccc agcttcatga tgaacgcacc ggtattgagc tggccaatcc
     6361 ttttttgtac ggtctgcggt ttgattttaa gccgcatcct tatctggaag tgggcttgtc
     6421 gcatctttcc atgttcggcg gaccgggacg acgcgatttg agtatcggcg atgtgctcaa
     6481 agttctgtac agcaatacca atcccgatcc ttctgaaaaa ctggacagca acgcccaggt
     6541 cgctctggat cttgcgctga ctctgcccaa tgttaaaaaa tatattttca ttgccgacgc
     6601 tttaaagctg tacacggagt ggggcgcaga ggataccggc ttcccgccgg ataaacgcgc
     6661 ttacattggc ggactggcgc tgttcaaacc tttcgggctg gaaagagcgg ttttgcgcgg
     6721 cgagtatgcc agaatgtcgc ccggcagtct gcccggtgcg tggtattccc accctacgta
     6781 tcccatgcgt tataacggtc gtgttttcgg tcaccatgcc ggcaatgatt cggatgatat
     6841 cttcgtggaa tggtcgcaaa catttgataa gttttcttat aagctgagtt ttgaccgcga
     6901 gcgaagcggc atcggcacaa aaagttttgt gcagaccaaa aatcaatatc tgg
//
LOCUS       DAOSME010000092         6930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_12051_length_6930_cov_25.009745, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000092 DAOSME010000000
VERSION     DAOSME010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12051_length_6930_cov_25.009745"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /gene="gyrB"
                     /locus_tag="PLG98_10300"
     CDS             complement(<1..1729)
                     /gene="gyrB"
                     /locus_tag="PLG98_10300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOE33695.1"
                     /translation="MTHNYDADSIKVLEGLEAVRKRPAMYIGSTGKEGLHHLVYEVVD
                     NSIDEAVGGFCDTITVKIRVDNSIVVDDNGRGIPIDMHKTEGVSAAEVVMTKLHAGGK
                     FSNDSYKISGGLHGVGVSVVNALSKTCELEVRRDGNVYMQSYKRGIPDAPLEKIGKTR
                     GKGTKVTFLPDDEIFEETEFSYDIIANRLRELAFLNSGVKIILIDERANKQNEFFYKG
                     GIISFVEYINRNKKVLHKNPIYVSGAREDCSVEVALQYNETYLENIFSFANSINTTEG
                     GTHMIGFRSALTRVFNNYATNNKILKDGKESLRGEDLREGLACVISVKVKNPQFEGQT
                     KTKLGNSEVRGLVEGIIYDKIGSYLEENPSVAKQILNKCMEAARAREAARRARELTRR
                     KSALEVGALPGKLADCQEKDPALSEIYLVEGDSAGGSAKQGRDRKNQAILPLRGKVLN
                     VEKARFDKMLQNEEIKTMVTAFGAGIGADDYDISKLRYHKVIIMTDADVDGAHIRTLL
                     LTFFFRQMRELIEKGYLFIAQPPLFKVVEKKQEFYLQNEEQMKNYILENGVGKVKLMM
                     GDGSPFPVNG"
     gene            complement(1757..2866)
                     /gene="dnaN"
                     /locus_tag="PLG98_10305"
     CDS             complement(1757..2866)
                     /gene="dnaN"
                     /locus_tag="PLG98_10305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOE33696.1"
                     /translation="MEFKINREIFLDGIQKTLGIVEKKTTIPILNNILIKAEANKIKI
                     IATDREIILISDYEAEVMEKGEITISAKKIYEMIREIQGEFINFIQKNNLVKISAQRA
                     FYKIPGLPADDFPSIADDQDIPFSKIQGSIIKDLINKTSFAMATDETRKNLNGVLMEA
                     GLDGSNYLLRMVATDGHRLAMARGFTPEPTLKTVKGIIIPRKGLMEIKKIIDENKEVQ
                     IGLHKNMFLVKTENTLLKVSLVDADYPDYKKVIPTEKGMSVVLEKDYFLHALRRMSVV
                     SSERYGGVILSFSKGKLTLNSTNLDVGEATEEIDIDYNGEAIDSGFNVNYLIDSIAVV
                     NKENIVFEVGLGLKPSIIKQAEDDNYLCIIMPLKI"
     gene            complement(3018..4391)
                     /gene="dnaA"
                     /locus_tag="PLG98_10310"
     CDS             complement(3018..4391)
                     /gene="dnaA"
                     /locus_tag="PLG98_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOE33697.1"
                     /translation="MFVDKGFNMDAFWDKTSKIIQEKISKQNFDTWIKPIQIVAMEEQ
                     CVKLAVPNKFFRDWLVDNYSSTIKQSLKDASGLDVNIDFILSKTKETDHEPKAKQTAA
                     AKTAAPAVHSRSKTFSFLNDNYSFERFVVGSCNQFAHAASVAVAKQPAKNYNPLFIYG
                     GSGLGKTHLLNAIGLMANSIHPSLNIMYVSAEEFMNEMINSIRYDRMPKFREKYRNIS
                     CLLIDDIHFLAGKDRTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEHRLRSRFEW
                     GLIADIQPPEIETKIAIIERKIQENKIELPNNVIHYIASHVESNIRELEGFLVRIGAY
                     SSLTGREIDLDLVKEVLSNLVKHNHKGEISIEEIMKVISGKLNIKISDIKAHNKNKNI
                     VFARQIVMYLARKLTNQSFPDIGQKIGGRDHSTVIYANNKIANNIKSDIKLKNLIQEI
                     EDILIHK"
     gene            complement(4426..5283)
                     /locus_tag="PLG98_10315"
     CDS             complement(4426..5283)
                     /locus_tag="PLG98_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOE33698.1"
                     /translation="MAQKNVLGKGLGAIFPDLINNESDKKKSLYCGIEELHPNRYQPR
                     KTFHDDKQKELIASIKSSGIIQPIIVRKTNSGYEIIAGERRWRAAQAAGLKDVPIVIR
                     EASDREAAELSLIENIQREELNPLEEAGAYVTLMDQFKLSQEDISARVGKDRSTISNT
                     IRLLKLPAKIQSALMANQISAGHARCLLACPSPEEQIAALTLILKKDLNVRETERLIQ
                     RAKKIPLDKKQPVKKDRFLNDLEKAMTVKLQAKVQINGSDKKGLIEIRYTSMDELNRL
                     SGLILDDLQ"
     gene            complement(5284..6051)
                     /locus_tag="PLG98_10320"
     CDS             complement(5284..6051)
                     /locus_tag="PLG98_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE33699.1"
                     /translation="MNKIICIANQKGGVGKTTTAINLSASLAAAEKKTLLIDGDSQGN
                     STSGVGVDRAVLETANLYHAMIGDVPLKDVIMHTAIPHLDVVPSTQDLIGIEVEFVGL
                     EDKEKRLKQLLNDLDARYDFIVIDCPPSLGVMTVNALVAADYLIVPLQCEYYAMEGLG
                     YLLNTVKLVKAQLNPHLVLGGILLTMFDQRNLLSHQVSDDVRKHFGDKVFKTVIPRNV
                     RLSESPSHGLPVILYDIKSRGAMAYMEMAKEIIQGGL"
     gene            complement(6136..6873)
                     /gene="rsmG"
                     /locus_tag="PLG98_10325"
     CDS             complement(6136..6873)
                     /gene="rsmG"
                     /locus_tag="PLG98_10325"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOE33700.1"
                     /translation="MTRWTPMTGHEYNLKFAAVDLFCYCTGMNHDIAFLKNQARQLNI
                     ALSNGQLQQFDVYKNELMDWNAKTNLISEKSARDIIARHFLDSLTALRYITPPDAKII
                     DVGSGAGFPGLPLKIAQPSLDLYLLEANRKKVSFLKHIVRLLNLSSVDIIHDRVENII
                     RENVYQEKFDVLISRASFKLPQLCSFCRYFLTPAGRLIALKGPGVEAEIQKCFEDRNP
                     SGIYQLIQQPIEAPFLNSTRKIIIGQR"
BASE COUNT         1769 a         1621 c         1420 g         2120 t
ORIGIN      
        1 tgccgttgac cggaaacggc gagccgtcgc ccatcatcag cttgaccttg cccacgccgt
       61 tttccagaat ataatttttc atctgttctt cattctgcag atagaattcc tgctttttct
      121 ccaccacttt aaacagcggc ggctgcgcga taaacaaata ccccttttca atcaactccc
      181 gcatctgccg gaagaagaag gtcagaagca gcgtgcgaat atgcgcgccg tccacatccg
      241 catccgtcat gatgatgact ttatgatacc gcaatttact gatgtcgtaa tcgtcggcgc
      301 cgataccggc tccgaaagcc gtcaccattg ttttgatttc ctcattctgc agcattttat
      361 cgaaacgcgc tttctctacg tttagaactt taccgcgcaa cggcaatatc gcctgattct
      421 tacggtcccg gccctgcttg gccgaaccgc cggcggaatc gccctccacc agatagattt
      481 cgctaagcgc cggatccttt tcctggcaat cggccagttt gcccggcagc gcgccgactt
      541 ccaaggcgct tttacggcgg gtcagttccc gggcccgtct ggccgcttca cgggcccgag
      601 ccgcttccat acacttgttg agaatctgtt tggccacgga ggggttttct tccaggtagc
      661 tgccgatttt atcgtagatg atgccttcca ccaggccgcg gacttcgctg ttgcccagct
      721 tggttttcgt ctgaccttcg aactgcggat ttttcacctt gacactgatc acacaggcca
      781 gcccttcccg aagatcttcg ccgcgcagcg attctttgcc gtctttgaga attttattgt
      841 tggtcgcgta attattgaac acgcgggtca gagcggaacg aaatccgatc atgtgcgttc
      901 cgccttcggt ggtgttgatg ctgttggcaa atgaaaaaat gttttccagg tacgtttcat
      961 tgtactgcag cgctacttcc acagagcagt cttcccgtgc gcctgaaaca taaatcggat
     1021 ttttatgcag aactttttta ttgcgattga tgtattccac aaaggaaatg atgcctcctt
     1081 tgtagaaaaa ttcattctgc ttgttggcac gttcatcaat gagaataatt ttaacgcctg
     1141 aatttaaaaa ggccaattcg cgcaaacgat tggcaataat atcgtagctg aattccgttt
     1201 cttcaaagat ttcatcatcc ggtaaaaaag tgactttggt gcccttcccc cgggttttac
     1261 cgatcttttc cagaggagca tccggaatac cgcgtttgta ggattgcata taaacattgc
     1321 cgtcccgacg cacttccagc tcgcaggttt tggaaagcgc gttcacaacg gacacgccca
     1381 cgccatggag acctccggat attttatagg agtcattgga aaatttcccg ccggcgtgca
     1441 gcttggtcat gacgacttct gccgcagata cgccttccgt tttatgcata tcaatcggaa
     1501 taccgcggcc attgtcatct acgacaatac tgttatccac cctgattttc acggtaatgg
     1561 tatcacaaaa accacctacg gcttcatcaa tactattatc aacgacttca taaacaagat
     1621 ggtgaagacc ttctttaccg gtggaaccta tgtacattgc cggccgctta cgaacagctt
     1681 ccagaccttc taatacctta atactatccg catcataatt atgtgtcatg tttgattttc
     1741 tgcttcctct ctgaaattat attttcaacg gcataataat gcataaataa ttatcatctt
     1801 ccgcctgctt aatgatggac ggtttcaatc ccaaaccgac ttcaaaaaca atattctctt
     1861 tattgacgac agctatggaa tcaattaaat agttgacatt aaatccactg tcaatcgcct
     1921 cgccattata atcgatatcg atttcttccg tggcttctcc aacatccaga ttcgttgaat
     1981 tcagtgttaa ttttcccttg gaaaaagaaa gaatcacccc gccgtaccgt tcggaagata
     2041 caacgctcat tcttctcaac gcatgcagaa aataatcttt ttccagtaca acactcatcc
     2101 ccttttcggt tggaatcact tttttataat caggataatc ggcgtccaca agactgacct
     2161 tcagaagggt attttccgtt ttgaccagaa acatgttttt atgaaggccg atctgaacct
     2221 ctttattctc gtcaataatc tttttgattt ccatcaaccc tttacgggga ataataatcc
     2281 ctttgaccgt ctttaacgtc ggctccggtg taaatcccct ggccatggcc agacgatgcc
     2341 catccgttgc caccatccgc aataaataat tcgagccatc cagtcccgct tccatcaaga
     2401 caccgtttaa attttttctt gtctcatccg tcgccatggc aaaagacgtc ttgttgatta
     2461 gatctttgat aatacttccc tgaatcttgg aaaacggtat atcctgatca tcggcaatac
     2521 tcgggaaatc atcagcaggc aatcccggta ttttataaaa agctctttgc gcggatatct
     2581 tcacaagatt attcttctga ataaaattaa taaactctcc ctgtatttca cggatcattt
     2641 cgtatatctt cttggcggaa atggttattt ctcccttttc cattacttcc gcttcgtaat
     2701 cggaaattaa aataatctct ctatctgtgg caataatttt gattttattg gcttcagctt
     2761 taataaggat attatttaaa ataggaattg tggttttttt ctcaacaata cctaaagttt
     2821 tctggatccc atcaagaaag atttctcgat tgattttaaa ttccatatag cctccttatt
     2881 tttagtaatt ctttttataa ataaaaaaat agtattaata gtatagtttg tttatatgtt
     2941 gataaatgtt ttttctttaa ttaaattcat attatatttt tcttccgcct gtttataatt
     3001 tttgtattaa aagaggatta tttgtggatt aaaatatcct ctatttcctg aataaggttt
     3061 ttcaacttta tatccgattt tatattgttt gctattttat tgtttgcata aatcactgtg
     3121 gagtgatcac gaccgccgat tttctgcccg atgtcgggaa aagattgatt ggttagtttt
     3181 ctggctagat acataacaat ctgacgtgca aaaacgatat tcttattttt attatgggct
     3241 ttgatatccg agattttaat atttaatttt ccggagatga ccttcataat ctcttcaata
     3301 ctaatttccc ctttatgatt gtgtttcacg agattactca gaacttcctt aactaaatcc
     3361 aggtcgattt cccggcctgt cagcgaggaa taagcgccga tacgcaccag aaaaccttcc
     3421 agttcgcgaa tgttggactc aacatgagac gcgatgtagt gaatgacatt attgggcagt
     3481 tcaattttat tttcctgaat tttccgttca atgatggcaa ttttggtttc aatttcaggc
     3541 ggttgaatat cggcaatcag gccccattcg aagcgtgaac gcagtctatg ttccaggttg
     3601 ggaatatctt tgggaaattt gtcgctggtg acgacaattt gttttccgga gtcgtgcagg
     3661 gtgttgaatg tatggaaaaa ttcttcctgg gttctgtctt tgcccgccag aaaatgaatg
     3721 tcatcgatca acaggcagct gatattccgg tatttttccc gaaacttggg cattctgtcg
     3781 taacggatgg agttgatcat ttcattcata aattcttccg ccgaaacata catgatattg
     3841 agtgacggat gaatggaatt ggccatcaga ccgatcgcgt ttaataagtg tgtttttcca
     3901 agtcctgacc cgccgtaaat aaaaagagga ttgtaatttt tggcgggctg cttggcaacc
     3961 gcaacggagg cggcatgggc aaattgatta caggacccga ccacgaatcg ttcaaaactg
     4021 taattatcgt ttaaaaaaga aaaggtttta ctgcggctat ggactgccgg agcagccgtt
     4081 ttggcggcag cggtctgttt tgctttgggc tcgtgatcgg tttctttggt tttggaaagg
     4141 ataaaatcaa tattcacatc aagaccggaa gcgtccttta gcgattgttt aatggttgag
     4201 gaataattat ccaccagcca gtccctgaaa aatttattcg gtacggccaa cttgacacac
     4261 tgctcttcca tcgccacaat ttgaatgggc ttaatccacg tgtcaaaatt ctgcttactt
     4321 attttttcct gaatgatttt actagttttg tcccaaaacg cgtccatatt aaaaccttta
     4381 tcaacaaaca tatccacagc tgttgatata cctaattaac ctgatttact gtaaatcgtc
     4441 aagaattaaa ccggacaagc ggttcaattc gtccatagac gtataccgga tttcaatgag
     4501 cccttttttg tccgatccgt tgatttggac tttggcctgt aattttaccg tcatggcttt
     4561 ttccaaatcg ttgagaaacc ggtctttttt aacaggctgt tttttgtcta gcgggatttt
     4621 tttcgcccgt tgtatcagcc gttccgtttc ccggacattt aaatcttttt ttaagatcag
     4681 ggtcaaagcg gcaatctgtt cttccggtga aggacatgcc agcaaacagc gcgcatgacc
     4741 ggccgaaatc tgattggcca tcagagcgct ttgtatttta gccggcaatt ttaacagccg
     4801 aatggtgttg gaaatcgtcg accggtcttt tccaacccgt gcggaaatgt cctcctgtga
     4861 aagcttgaat tggtccatca gggtcacata agcgccggct tcttcaagcg gatttaattc
     4921 ttcccgctga atgttttcaa tcagcgaaag ttctgccgct tccctgtcgc tggcttcacg
     4981 gattacaatc ggtacatctt ttaaacccgc agcctgagcc gccctccagc gccgttcgcc
     5041 ggcgataatt tcgtacccgc tattggtctt acggacgata atcggttgaa tgatcccgct
     5101 gcttttgatc gaggcgatca attccttctg tttatcatca tgaaaagttt tacggggctg
     5161 ataacgattg gggtggagtt cttctatgcc gcagtacaaa ctttttttct tatcggattc
     5221 attgttgatt aaatcgggaa atatagcgcc caagcctttt cccagcacat ttttttgtgc
     5281 catttacaat cccccttgga tgatttcctt ggccatttcc atataggcca tcgcgccgcg
     5341 cgatttaata tcgtaaagaa tgacgggcag tccgtgactg ggactttccg agagccgcac
     5401 gttacgcgga atgaccgttt taaatacttt gtcaccgaaa tgtttgcgca catcatcgga
     5461 cacctgatgc gacagcaaat tgcgctgatc gaacatggtg agcagaatgc cgcccaaaac
     5521 cagatgggga ttcaattgcg ccttgaccag tttaacggtg ttgagaagat accccagacc
     5581 ttccatcgca taatattcac attgtaaagg aacaatcaaa taatcagctg cgaccaaagc
     5641 gttgactgtc ataacgccca gggacggcgg gcaatcgatg acgataaaat cataacgcgc
     5701 gtccagatcg ttcaataact gtttcagccg tttttcttta tcttccagac cgacaaattc
     5761 cacttcgatg ccgatcagat cctgtgtgga cgggacaacg tccagatgcg gaattgccgt
     5821 gtgcatgatc acgtctttga gcggcacatc gccgatcatg gcatgataca aattggccgt
     5881 ctccaaaacc gctctgtcaa ccccgacacc gcttgtggaa ttgccctgag agtcgccgtc
     5941 aatcagcaac gttttttttt ccgcggcagc cagagaggcg gataaattta ttgcagtcgt
     6001 ggttttcccg acaccaccct tctggttcgc tatgcatatg attttattca tttattattt
     6061 agtatcgcaa acaatgttct ttagcaggaa ctgtggatag cactagcata aaaaaatgct
     6121 ttttagaagg attttttatc tttgcccgat aataattttg cgcgttgaat tcaaaaaagg
     6181 agcctctata ggctgttgaa tcaattgata aattccggat ggatttcggt cctcaaaaca
     6241 tttttgaatt tccgcctcca ccccgggacc ttttaaggca atcaaccggc ctgcgggggt
     6301 gagaaaatac cggcagaatg aacacagttg cggcaatttg aacgaggccc gggaaatcag
     6361 aacgtcaaat ttttcctgat aaacattttc ccggatgatg ttttcaacgc ggtcatgaat
     6421 aatatcgact gaagaaagat taagcagccg cacgatgtgt tttaaaaagg acactttctt
     6481 gcgattggct tccagaagat aaaggtccag cgaaggctgc gctattttta agggaagacc
     6541 gggaaacccg gcgccgcttc ccacatcaat gatttttgca tcaggcggtg tgatgtacct
     6601 caaggccgta agagaatcca ggaagtgtcg ggcaataata tcgcgggccg atttttcaga
     6661 aatcagattt gtcttggcgt tccagtccat caattcgttt ttatagacgt cgaactgctg
     6721 cagttgcccg ttcgaaagag cgatatttaa ttgccgggcc tggtttttca aaaacgctat
     6781 gtcatgattc attcctgtgc aataacaaaa aagatcaaca gccgcaaatt ttaaattata
     6841 ttcatggcct gtcatcggcg tccatcgtgt cattacaagg gctctccccg ataagattcg
     6901 cttgacgcac cagcgcggag gtcatatact
//
LOCUS       DAOSME010000093         6861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_12203_length_6861_cov_22.934617, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000093 DAOSME010000000
VERSION     DAOSME010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12203_length_6861_cov_22.934617"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLG98_10330"
     CDS             complement(<1..212)
                     /locus_tag="PLG98_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33701.1"
                     /translation="MQAKTNILNLPTQEFVHPGTRACTGCGLSIAYRVGLKALGKDTI
                     LVVPPSCLTVLQGLFPVASTKLPCLNV"
     gene            complement(263..1456)
                     /gene="porA"
                     /locus_tag="PLG98_10335"
     CDS             complement(263..1456)
                     /gene="porA"
                     /locus_tag="PLG98_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOE33702.1"
                     /translation="MIENGNTAAALGVKLCRASVIAAYPITPQTPLTEKLSEYVELGD
                     MKAEYIPVESEHSALAVCIAASSAGARVFTATSANGLLYMHEQIQWASGARLPIVMCV
                     VNRAVGAPWNVWNDQQDSISQRDTGWIQIYCCDHQQIIDTVIKAYWLAEKISIPIMVC
                     YDGYILSHTYMPFDLPEQEKVDAFLPPFQPDYALNPDEPANLNTVTLPDVRIDTKGQL
                     AHGYMEIRYLLQEELRTTLQIAADAEKRFAEIFGRGGNPYIEPYRCDDADYVAVGLGS
                     ITYQLRVCVDTLRKEGIKVGVMGVRYYRPFPDAVIAGALKGKKGIIVFEKALSYGYEG
                     ALASDLKSALYEHLAGTGSLPAVQNLIAGIGGREIHTDDLTQNLRAATQGRISREPAW
                     IGLKI"
     gene            complement(1468..>1763)
                     /locus_tag="PLG98_10340"
     CDS             complement(1468..>1763)
                     /locus_tag="PLG98_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE33703.1"
                     /translation="RKTSHSEHGPGDGGRTGSWRVERPVLDQSLCIPCKRNKEACFIC
                     WLYCPEIVISRTIPPQFNLEYCKGCGICAEECPTKAITMVDEAHFSEQKKDKE"
     assembly_gap    1764..1863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1901..2461)
                     /locus_tag="PLG98_10345"
     CDS             complement(1901..2461)
                     /locus_tag="PLG98_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOE33704.1"
                     /translation="MNRDFIEIRWHGRGGQGAITAAKIVAGAAFVSGYEGVVMAPTFG
                     TERRGAPVMTSLKISREKIYDLSPIETPDIVVVLDHLLLAEADVTHGLKEGGIIVLNT
                     PMAHDAYDFKNFRLATADVTAISVEAGLPHGMINTGIIGSLEKATTLLGMDILIQGIE
                     EEFRTRKPQKNSLAAKIAYERTVTGG"
     gene            complement(2661..>3650)
                     /gene="hflX"
                     /locus_tag="PLG98_10350"
     CDS             complement(2661..>3650)
                     /gene="hflX"
                     /locus_tag="PLG98_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOE33705.1"
                     /translation="CGVEVFDSMIQYRPQPDPRFMVGRGKLSAIDLRASQIGANMLIF
                     DHDLTPAQARSISDFTGLKIIDRTQLILDIFARRAHSREGKIQVELAQLKYRLPRLTH
                     ADTSLSRLAGGIGGTGPGETKLEIDRRRVRERIHRLDKDLKTITKSRRQRYGRREKTG
                     LPIISIVGYTNAGKSTLLNTLTQSSVLAEDKLFATLDTKSARLRFPRDTEAIITDTVG
                     FIRDLPEELFTAFRATLDELFEADILLHVIDASNAQFEDQIAAVEKILTDLEIHDRPT
                     IRVLNKSDRVADKELLKHLCRRLDAVAVSALDKKTLFALMEKIESVLPRKDDF"
     assembly_gap    3651..3750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3751..4195)
                     /locus_tag="PLG98_10355"
     CDS             complement(<3751..4195)
                     /locus_tag="PLG98_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOE33706.1"
                     /translation="MRFKGLRLIHTHLNGENLSDEDMTDLSHLRLDLIGALDVREDGS
                     PGLLHWAHLVPENPAGDYWLFMKPEEPHQIKINFLSFIQALEDEFAKKQQSRKIDATN
                     KAILLRVEKNPLAGAEASLEELAQLAHTCGVEVFDSMIQYRPQPDP"
     assembly_gap    5072..5171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5172..5873)
                     /locus_tag="PLG98_10360"
     CDS             complement(<5172..5873)
                     /locus_tag="PLG98_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33707.1"
                     /translation="MKKLWLVLLSLGLLMAFSVSASAADVKFSGEYYAAGLYMNHVNL
                     QDPALTYSRSTAFFYQRLRLGTDFVVSPCLRLVTRMDIMERVWEPDNTGNDAFGRPAA
                     PGITNDARNLDFDVVYIDYVSPIGKFQAGYMPDYVWGTIWGNRATGPTTGQIKYFLPI
                     GPVVLFAGFAKEDENDYSVQYPGAVETDRDQNSYRVGPIFQFKGNNASGEAGVLFTYD
                     DVRQYGAVFEQDVFTI"
     gene            6638..>6861
                     /locus_tag="PLG98_10365"
     CDS             6638..>6861
                     /locus_tag="PLG98_10365"
                     /inference="COORDINATES: protein motif:HMM:NF027503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOE33708.1"
                     /translation="MADAKVQLKEIYPIPEKAKKKAWISGRDAYDKLWKKSVEKPEEF
                     WAEIAEQQVTWFKKWDKVMDYNFDIKKGPI"
BASE COUNT         1557 a         1624 c         1699 g         1681 t
ORIGIN      
        1 acattcaggc agggcagttt tgtggacgcc acggggaaaa gcccctgcaa aacggtcagg
       61 caactgggcg gtacgaccag aatcgtatcc ttcccgaggg ccttgagtcc gacccggtag
      121 gcaatggaaa gtccgcaacc cgtgcaggcc cgtgtgcccg gatgaacaaa ttcctgagtc
      181 ggtaaattta aaatgtttgt cttcgcctgc atttgcctct cctgacctcc atcaccggga
      241 tgcgtttgtt attgcggatg tgctaaatct tcagaccaat ccacgccggt tcccgggaaa
      301 tcctgccctg tgttgccgct ctgagatttt gtgtcaaatc atcggtatga atttcgcggc
      361 cgccgatgcc ggcaatcaaa ttctgcacgg ccggcagaga gccggttccc gccagatgtt
      421 cataaagcgc cgatttcaga tccgacgcca gcgcgccctc gtagccgtag ctgagcgcct
      481 tttcaaaaac gatgattccc ttcttccctt ttaatgcgcc ggcaataacg gcatcgggaa
      541 aaggacggta ataacgaacg cccatcacgc cgacttttat gccctctttt ctcaatgtgt
      601 ccacgcagac gcgcaattga taggtgatgg atcccaggcc gacggcgaca taatcggcat
      661 cgtcgcagcg gtagggttct atatacggat tcccgccccg cccgaatatt tcggcaaatc
      721 gcttttctgc atcggctgcg atttgcagcg ttgttctcaa ttcctcctgc agcagatacc
      781 ggatttccat ataaccgtga gccaactggc ctttggtgtc gatccggaca tccggcaggg
      841 tgacggtgtt caggttggcc ggttcgtcgg gatttaaggc gtagtccggt tgaaacggcg
      901 gcaggaaggc atcgaccttt tcctgctcgg gcagatcaaa cggcatgtag gtatgggata
      961 aaatatatcc gtcgtagcag accatgatcg gaatgcttat tttttccgcc agccagtagg
     1021 ctttgatcac cgtgtcgata atctgctgat gatcgcagca gtagatctga atccagccgg
     1081 tgtcgcgctg agagatggaa tcctgctggt cgttccagac attccaggga gcgcctaccg
     1141 cgcggttgac cacacacatc acgatgggca gtcgcgcgcc ggaggcccac tgaatctgtt
     1201 cgtgcatgta caaaagaccg ttggcgctgg tggcggtaaa aacgcgcgcg ccggcggatg
     1261 atgccgcgat gcacaccgcc agcgcggaat gttcgctttc caccggaata tattcagcct
     1321 tcatgtcccc cagttccaca tattcggaga gcttttccgt cagcggcgtc tgcggggtga
     1381 tgggataagc cgcgatgaca ctggcacgac aaagcttgac tccgagggct gccgcggtat
     1441 ttccgttttc gatcatgtgc atggcggcta ctccttgtcc tttttttgtt ctgagaaatg
     1501 cgcttcgtcc accatcgtga tggctttggt cggacattct tcggcgcaga tgccgcagcc
     1561 cttgcagtat tccagattga actgcggcgg tatcgtccgc gaaataacga tttccgggca
     1621 gtaaagccag cagataaaac aggcttcctt gttgcgcttg caggggatgc agagactttg
     1681 gtccagcacg ggacgttcca cccgccagga gccggttctg ccgccgtcgc cgggaccgtg
     1741 ttcgctgtgc gatgtttttc ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnntgcgatg tttttcttct gtaatttctg gtgcccatga tcagcctccc gtcaccgttc
     1921 gctcgtaggc gattttcgcc gccagcgaat ttttctgggg tttgcgtgtg cggaattcct
     1981 cctcaatgcc ctggatcaat atgtccatgc ccagcagggt cgtggccttt tccagcgatc
     2041 cgatgatacc ggtgttgatc atgccgtgag gcagtcccgc ttccacggaa atggctgtga
     2101 catccgcggt ggccaggcga aaatttttaa aatcataggc gtcgtgcgcc atcggcgtgt
     2161 ttaaaacaat gatgcctccc tctttcaggc catgcgtgac atccgcctcc gctaaaagca
     2221 gatgatcgag gacgacgaca atgtccggcg tttcgatggg agacaaatcg tagatttttt
     2281 ccctggatat tttcagcgat gtcatcaccg gcgctccccg gcgctccgtg ccgaatgtcg
     2341 gcgccatgac cacgccctca tagccggaga caaaagccgc tcccgcgacg atcttcgccg
     2401 ctgtgatggc gccctgtccg ccgcgtccgt gccagcggat ttcaataaaa tccctgttca
     2461 tgcgatattc cttacgttcc ggggttgcct ttcagcgatt gagcttggag gacaagcttt
     2521 tctggcatta tgggggaggc tgcaaccagg ctccggccgt ctttgccatg ccggacattt
     2581 catatataca aaatactcaa aaaaacgcaa aagtcaaaaa atcatttgca cgggattcca
     2641 ataacacaaa atcggcaaag ttaaaagtcg tctttacggg gaagaacaga ttctattttt
     2701 tccatcagcg cgaaaagcgt ttttttatcc agagccgaga cggcaaccgc atccagcctg
     2761 cggcagagat gtttcagcag ctccttgtcg gcaacacggt cggatttgtt cagcacccgg
     2821 atcgtcggcc tgtcgtggat ttccagatcc gtaagaattt tttccaccgc cgcaatctga
     2881 tcctcgaact gagcgttgct ggcgtcaatg acatgcagca ggatatccgc ttcgaacagt
     2941 tcgtcaagcg tcgcgcggaa ggcggtgaat aattcttccg gaagatcgcg gataaatccc
     3001 accgtgtcgg tgatgatggc ttccgtgtcc cggggaaaac gcagacgcgc gcttttcgtg
     3061 tcgagtgtcg caaacagttt gtcttccgcc aggacggaac tttgcgtcag cgtgttgagc
     3121 aatgtggact tgcccgcgtt ggtataaccg acgatggaaa taatcggcaa tcctgttttt
     3181 tcccggcgac catagcgctg acgccgtgat tttgtgatgg tctttaaatc tttgtccaga
     3241 cgatgaatgc gctcgcggac gcggcggcgg tcgatttcca gtttggtttc cccgggaccg
     3301 gtgccgccga tgccgccggc cagccgcgac agcgacgtgt cggcatgcgt cagccggggc
     3361 agccggtatt tgagctgtgc cagttccacc tgaattttcc cttcccggct gtgggcgcgc
     3421 cgggcaaaaa tatccagaat gagctgtgtg cggtcgatga ttttcaggcc cgtaaagtcg
     3481 ctgattgaac gcgcctgcgc gggtgtcaga tcgtgatcga agatgagcat gttggcgccg
     3541 atctgcgaag cgcgcagatc gatcgcggac agcttgcccc gtcccaccat gaacctggga
     3601 tcgggctggg gccggtactg aatcatggaa tcgaaaacct ccacaccgca nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgggatcggg ctggggccgg tactgaatca
     3781 tggaatcgaa aacctccaca ccgcaggtat gcgccagttg agccagttct tccagcgatg
     3841 cttcggcacc ggccagcgga tttttttcga cccgcagcag aatggcttta ttcgtcgcat
     3901 caattttacg cgattgctgt tttttggcaa actcatcctc cagcgcctga atgaaggaaa
     3961 gaaaattaat tttgatctgg tgcggttcct ccggtttcat gaaaagccag tagtctcccg
     4021 ccggattttc cggaaccagg tgcgcccagt gcaacaggcc cggtgatccg tcttcccgga
     4081 cgtccagcgc gccgatgagg tccaggcgca gatgggacag gtccgtcata tcttcatcgg
     4141 atagattttc accgtttaaa tgtgtgtgaa tgaggcgcag tcccttaaaa cgcactgaag
     4201 aagaacggaa tccgtccagc gatggaatga atatgctttt gtaatcgccg agaataactt
     4261 tgctgatttc gcccttgcgg ctgatcagca ggccgatttg ccggttgatt tcacgggaca
     4321 gcgaagagac ctgccgggca agatcattcg taatgatgtt ttccggcggg atacctttat
     4381 atccgaggcg ctccagttgt ttgatgtgcg ccgcttttaa tcctgtcgta tttccggaaa
     4441 ttttgttgat ggcagtggtc ctgttaaaaa tattttcaca tcacattctt cgcggccagt
     4501 ggaggatgtg atgcgttgaa attgctttac cacattttgc aggatttagt caaaagatgt
     4561 gaagggcagt tgatgattga tggttgatgg tgaatagtga atagtgaata gtgaatagtg
     4621 aatagaaaaa acggttctgg attcaaaatt caagctgcat cccaccgaat aggggcttgt
     4681 taccggcgag ggtcatttcc taatgtcgct gcactcctag gatagttcgg cttactagat
     4741 tcggtggcag gcgtttataa aatgaagttc acaatcccac tccgcttcgc accaagcggg
     4801 tctcatttcc tacgtcgtcg aagatgccct ttagggtact tcgctcctag gataattcca
     4861 ctagcgcttc aagcgagtgt cacttcccac gtccctgcgg gactgggata gttcgctcta
     4921 agattatagt caaggctcat taccccacgt cgctgcgctc ctggggtaat tcgtccagcc
     4981 agatattact gcgatgttgt tgattcttaa attcaggaat cccgctaaag cgggatagtt
     5041 caaccatcaa gttctagtgc gcttcgcttc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn naattgtgaa cacgtcttgt tcaaaaacgg ccccatactg acggacatcg
     5221 tcgtaggtga acagaacgcc tgcttcaccg gatgcattgt tgcccttaaa ttgaaaaatc
     5281 gggccgacgc ggtaagaatt ctgatccctg tcggtttcta ctgcgccggg atactgcacc
     5341 gagtaatcat tttcgtcttc ctttgcaaat ccggcaaaca acacaacggg tccgatcggc
     5401 aggaagtatt tgatctggcc tgtggtgggg ccggtggctc tgttgcccca gatggttccc
     5461 catacgtaat ccggcatata accagcctgg aatttgccga tgggagaaac ataatcaatg
     5521 tagacgacgt caaagtccag attgcgggca tcattggtga tgccgggagc agctggtctg
     5581 ccaaatgcat cattccccgt gttgtctggt tcccagactc tttccataat gtccatgcgg
     5641 gttacgagtc tgaggcaggg cgatacaaca aagtcggttc ccaggcgcag tctctgatag
     5701 aaaaaggctg tgctgcggga atacgttaat gcgggatcct gtaaattaac atgattcata
     5761 tacaggccgg cggcataata ttcgccgctg aatttaacgt cagctgcgga agctgacaca
     5821 ctgaaggcca tgagtagccc cagtgacaac aaaactaacc aaagtttctt catgttttcc
     5881 tccttaaata aagagtcatt aattaccgct tttcagaaaa cgggcatttt cttcaagcga
     5941 cttccacaca ctgaataaat tacgggaaaa actcccgtga attacagtca aaaatacgtc
     6001 tgtattaaaa tagcactgaa taattcattt tgtcaagaat gtttttacgt tcgaaaatgt
     6061 tttcaaataa acccaaagac gaatcataaa aatttgatca tttgaaaatg gagacaggtg
     6121 tttttgtgtg cggataaaat taatctctcg ctttgcgctt acagtgtcca cctgattgag
     6181 gtcgtacgat acatagtaaa gcagaaacgg ttttgtttga attccatcgc atcgaatcaa
     6241 gggggttgaa gtttctgtat gaaggtatgg gccgtttttc ggaagtttga atatttgcca
     6301 aaaacccaac ggaaaaagtc tggataatag cataatatta ttatgataat ttttaatttg
     6361 tgtttttgat ttttgattgt caaaaggcat ctgtaggcca gtccggcttt ttaaaaggga
     6421 aattcataaa ttgcaggacg tgatttttaa atatttcttg acatcaaata aatcttagga
     6481 taggaaaaat ccgaggcatc tctgactttc agtcaagttg tgacctttgt tcaaaaacac
     6541 cgttagtatc aaacagttag aatgtttgtt tggccggtat caaggcgtaa taaaaacgtt
     6601 tgaaatcata aaaattttta gggaggaata aattatcatg gctgatgcaa aagttcaatt
     6661 aaaggagata tatccgattc cggaaaaggc gaaaaagaaa gcctggatta gcggaagaga
     6721 tgcttacgac aagttgtgga aaaaatcggt cgaaaaaccg gaagagttct gggcggaaat
     6781 cgccgagcag caggtgacct ggttcaaaaa atgggacaaa gtgatggatt acaactttga
     6841 cattaaaaaa ggccccatct a
//
LOCUS       DAOSME010000094         6854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_12219_length_6854_cov_21.770260, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000094 DAOSME010000000
VERSION     DAOSME010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12219_length_6854_cov_21.770260"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLG98_10370"
     CDS             <1..795
                     /locus_tag="PLG98_10370"
                     /inference="COORDINATES: protein motif:HMM:NF005881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE33709.1"
                     /translation="AASGIGRATAAAMGLAGCRLFLTDINAEGLKKTADMISSAGDNV
                     CLSRPFDVGDYEAMRAFASEIHAEFGSLDILVNVAGVALFAKIEDMQHRDWEKIIRIN
                     LWGVIHGLECFVPEMIRAGKGGHVVSVSSTAGLIGLPWHVAYAATKHALVGMSEVLRY
                     DLRKYKIGVSVVCPGAVHTGLVNTADIRTDDHEATNKARKMFVKIAMTPEKVADLIIQ
                     AILKNKYLVITSLDVKLFYFIKRTCFPLYHLVMLIINRFMDKALKK"
     gene            822..1526
                     /gene="radC"
                     /locus_tag="PLG98_10375"
     CDS             822..1526
                     /gene="radC"
                     /locus_tag="PLG98_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOE33710.1"
                     /translation="MIQSESKHSKGINSWPEDDRPREKLLRQGAETLSNSELLAILLR
                     TGVKGASAIDLARRIVEKFGTFRNMIHTDVREWKEFKGLGPAKMAQIQAALEIGRRFR
                     EVEALKGKQKIASCCDVVEIVMPQMRDRKTELFKVLYLNSNNKIIDISDVAEGTVNHA
                     IPIVREIIHAALQKFAAAIICVHNHPSANISPSAEDRKFTQDLLAAGKVMGIKVLDHI
                     IIGDGNYYSFAEEGIL"
     gene            1614..2831
                     /locus_tag="PLG98_10380"
     CDS             1614..2831
                     /locus_tag="PLG98_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE33711.1"
                     /translation="MTYYIIIAFCVVILLAYFFDVTSRQTKIPGVLLLILTGMAINSI
                     DSYFNFEVSQIDVILPVMGTVGLILIVLEASLDLTLSKQKKSLILKSMSSAIVLFLVF
                     TGLFALMLSYGLGYDVRTSLVNIIPVAVISSAVAIPSAVGLSGNNREFVIYESSISDI
                     FGILAFNYVVISNGHLFSGILNFLLEIIVTLFASVLFSAFLAVILHKINHHVKYIIIM
                     TAIILVYALSRLLHLPSLLVVLIFGLVMNNNNLFENSITAQFINFQDFNTELNAFKNI
                     TSEMTFIVRSFFFVLFGYYTSLFDLLNLNNLLFSVLIVAAIYALRALYLKSIIRISMT
                     PLLYFAPRGLITILLFLSIPQAMALPYMNTGVVTQIIFITILIMAYGNIVFGKTGEAI
                     PTGTPQDKKNLSG"
     gene            3091..3408
                     /locus_tag="PLG98_10385"
     CDS             3091..3408
                     /locus_tag="PLG98_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33712.1"
                     /translation="MIIRKSAVVAVMLIALFLLSCGMSDPFSGKWFNDVIEKEMLTIT
                     KSGSAYKVDTALGSFPCIKEGDLLKCQMGIGDTMLQINENRCLVMKTPAGVEFKFRRQ
                     ENK"
     gene            3442..4509
                     /locus_tag="PLG98_10390"
     CDS             3442..4509
                     /locus_tag="PLG98_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE33713.1"
                     /translation="MIFENVALHNVDGLYKHKDIEGLLLGRIPEHVSARLSKAGQMMM
                     ICPSGSEIRFVSETYPVKITLSVDKITKHLGDGIITDARVFFGTFQTRQRFVIKRIKT
                     LLEIIRPPKFIELAEKIATDAPFSPHVCRIRFWGTTMGAPIRFHGIETEGKIRPPHAE
                     ELPGLSYLAYGTSLTQGAYASESHLSYPNLVGCRLGVDVINLGSSCSAYCEPEMADYI
                     AGRKDWSFATLDLSLNMVDTFSPEEFSRRVSYMINRIAGSNKSRPVFCITLKPYLGDY
                     IESKKPDLYRKLLRKAVENSALDNLYLIEGQDLMPDVAGGLCPDMVHLGDFGTIQIAE
                     KLAAKIKPVLQSHHLLFKHSR"
     gene            4692..5684
                     /locus_tag="PLG98_10395"
     CDS             4692..5684
                     /locus_tag="PLG98_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOE33714.1"
                     /translation="MYDHDTSKFQSVKEFIKHNYRHFNSAVVVDAAEAYANHINNGGK
                     MLMALAGAMSTAELGITLAAMIRSGKIHAVCCTGANLEEDLFNLVAHKHYKRVPNYRD
                     LKPEDEAELLRNKLNRVTDTCIPEEEAMRKVEGKLFSFWKKAEDENKRYFPYEFIMQI
                     ISSGVLKDQYEIDEKDSWLVAACEKNIPIFVPGWEDSSLGNYFVSSVRRKQLKHYSIV
                     KSGLETMDALIDWYLDASRHSETGFFQIGGGIAGDFSICVVPLIQQDMKMDCRLWSYF
                     CQISDSTTSYGSYSGAVPNEKITWGKIRPDSKKFIIESDATIVAPLIFAYVLAE"
     gene            complement(5712..6755)
                     /locus_tag="PLG98_10400"
     CDS             complement(5712..6755)
                     /locus_tag="PLG98_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOE33715.1"
                     /translation="MKDLFFEPVKGGKKPYLESLRKLLQHLRSPFAAGDTVGIKLHWG
                     EKGNKGFLPPDYAREIATWLIAHGTKPFVFDTTVLYSGGRRTAEDSLITAASHGYTHD
                     FLGCPVIIADGMDSRNLVDIPAGFNHFRTVQVANVFENAQGFVIFSHFKGHMVSLFGG
                     AIKNMSMGFASRAQKQRMHSDYHPVLNKKKCTRCGVCAEICPTGAATIATSDYPTYDL
                     KKCIGCAQCIAMCPEMALQIFWNSDMTVFQEKLVETAAAVWKIIKDKTIVINSLLNIT
                     VECDCLPGKNPVIHEDMGFIGGYHPVPVDAESLKMTGADIFEKVHPGIPWQRQFTYAR
                     EIGFSPARNITGF"
BASE COUNT         1797 a         1533 c         1675 g         1849 t
ORIGIN      
        1 gccgccagcg gaataggtcg tgctaccgct gcggccatgg ggctggcggg ctgccggctg
       61 tttctgacag atattaatgc tgaaggtctg aagaagacgg cggatatgat cagcagcgct
      121 ggtgacaacg tgtgtctgtc ccgtccgttt gatgttggcg attatgaagc catgcgggcg
      181 tttgcctcgg agattcatgc ggaattcggt tcgctcgata ttctggttaa tgttgcgggt
      241 gtggcgcttt ttgcgaaaat agaagacatg caacataggg actgggagaa aatcatcaga
      301 ataaatctgt ggggcgtcat tcacggtttg gaatgcttcg tgccggagat gattcgtgca
      361 gggaaaggcg gacatgtcgt ttcagtatcg tcgacggcag gtcttatcgg gcttccctgg
      421 catgtggcct atgccgccac aaaacatgct ctggtgggta tgtccgaagt attgcgatat
      481 gatttaagaa agtacaagat cggagtcagc gttgtctgtc cgggtgccgt ccatacaggg
      541 ttagtcaata cggcggacat tcgcacggat gatcatgagg caaccaataa agcacgcaag
      601 atgtttgtga aaatcgccat gacgccggaa aaagtcgctg atttgattat tcaggcgatc
      661 ctgaaaaata agtacctggt catcacgtca ctggatgtaa aattatttta ctttatcaaa
      721 aggacatgtt tccctttgta tcatcttgtc atgctgatca taaaccgttt catggataag
      781 gcgctcaaaa aataataaaa acgtatctga cggagtatca cgtgattcaa tctgaatcaa
      841 aacattcaaa gggcatcaat tcctggccag aggacgaccg tccccgggaa aagcttctgc
      901 ggcagggtgc agaaacactg agcaattcgg aactgctggc aattcttttg cgaacgggcg
      961 tcaagggtgc aagtgccatc gatctggctc gccggattgt ggaaaaattc ggcacgttcc
     1021 gtaacatgat tcatacggat gtgcgcgaat ggaaggaatt taagggattg ggacccgcca
     1081 aaatggcgca gattcaggcc gctctggaaa tcggccggcg ttttcgcgaa gtggaggcgc
     1141 tgaaaggaaa acaaaaaata gcctcctgct gcgatgtcgt tgaaatcgtc atgccgcaga
     1201 tgcgagatcg caaaacggaa ttatttaaag tcctgtatct taacagcaac aataagatca
     1261 tcgacatcag cgacgttgcg gaaggcacgg tgaatcatgc cataccgatt gtccgggaga
     1321 tcattcacgc tgcattgcag aaatttgcag cagccattat ttgtgttcat aatcatccca
     1381 gtgccaacat ttcgcccagt gcggaagatc gaaaatttac gcaggatctg ctggcggcgg
     1441 gaaaagtgat gggcatcaaa gttctggatc acatcatcat cggagacggg aactattaca
     1501 gctttgccga agaaggaata ctgtaaaatc gttaaaatta cgtccacgaa tgtgcgcccg
     1561 tttgattgga ctgcaaatca ccacatcgct tgctcgaaaa aggatcctct gcaatgacct
     1621 attacatcat tattgccttc tgtgtcgtta tcctgctggc gtattttttt gacgtgacat
     1681 cgcggcaaac aaaaattccc ggtgttctgc ttctgattct tacgggaatg gccatcaatt
     1741 ccatcgacag ttattttaac tttgaagtat cacaaattga tgtgatcctg ccggtcatgg
     1801 ggacggtagg gctcatcctg attgttctgg aagccagtct cgatttgacc ctgagcaaac
     1861 aaaaaaagtc cctgatcctc aagtccatgt cttcagccat tgttcttttt ttggtattca
     1921 cgggcttgtt tgcgcttatg ttatcctatg gtctgggtta cgatgttagg acctctctgg
     1981 ttaacattat tccagttgcc gtcatcagca gtgccgtagc gattccatcg gcggtcggtt
     2041 tgtcgggaaa taaccgggaa tttgtcatat atgaaagttc tatctctgat attttcggta
     2101 ttctggcatt caattatgtg gtcatcagta acggtcatct tttttcaggc attctgaatt
     2161 ttcttctgga aatcattgtc acattgtttg cctctgtgtt attcagcgca tttctggctg
     2221 tcattttgca taagatcaac catcatgtaa aatacatcat cattatgacc gcgattattc
     2281 ttgtctatgc gctgtccagg ttgcttcatc tgccgtctct gctggtggtc ctgatttttg
     2341 gcctggtgat gaacaacaat aatcttttcg aaaacagcat caccgctcag ttcattaatt
     2401 ttcaagactt caacacagaa ttgaatgctt ttaaaaatat tacgtcggaa atgaccttta
     2461 tcgtccgttc cttctttttt gtcctgttcg gatattatac gagccttttt gatttgctga
     2521 atttaaacaa tttattattt tccgttctga ttgttgccgc aatatatgcg ttacgagctt
     2581 tgtatttaaa gagcattatc cggatttcaa tgaccccgct gctttatttt gcaccgcgag
     2641 gcctgataac cattttactt tttttgagca taccgcaagc catggcgctg ccctatatga
     2701 ataccggcgt ggtgacgcag atcatattca ttaccatttt aatcatggcg tacggcaaca
     2761 tcgtatttgg gaaaaccggc gaggccattc cgaccggcac gccacaggat aaaaaaaact
     2821 tgtcgggatg aggaaggtat gcgcatatat ttcattgtac gcgccatgga tgcaaccaaa
     2881 ggcgatgaaa tcggtcgttg cattgcagga aatatccggt gtttcgcgca acggtaataa
     2941 tgatattgcg ccgcatacag aaatgccgct gcaaacgtta caggttaaat aacgcgatcg
     3001 ctgtgttgac aaaaaacttg catgtgatat cagctaaacg ctctgcgaca acgggcatcg
     3061 caatcagtat atttgaaaaa ggaggtccat atgattatca gaaaatctgc cgttgtggcg
     3121 gtgatgctca tagccctctt ccttttatcg tgcggaatga gcgacccgtt ttcgggtaaa
     3181 tggttcaacg acgtgataga aaaggaaatg ctgaccataa ccaaaagcgg cagtgcttat
     3241 aaagtcgata ccgctttagg atctttcccc tgtatcaaag agggggacct gttgaaatgt
     3301 caaatgggaa tcggagatac gatgctccag attaacgaga atcggtgtct ggttatgaaa
     3361 acaccggccg gcgttgaatt caaattcaga cgccaggaaa acaaataaag aattaagtca
     3421 atcctgaaaa cgggagaacg gatgattttt gaaaatgtgg ctcttcataa cgtagacggg
     3481 ctatacaaac acaaagatat tgaagggctt ctgttaggac gtattcccga acacgtttcc
     3541 gcacgtctca gcaaagccgg acaaatgatg atgatctgtc cttccggttc ggaaatccgt
     3601 tttgtcagtg aaacgtatcc tgtgaaaatc accctgtccg tggataaaat cacaaagcat
     3661 ctcggcgacg gaattattac cgatgcccgg gtttttttcg gaacctttca aacgaggcag
     3721 cgttttgtga ttaagagaat caaaaccctg ctggaaatca tccggccgcc gaaattcatc
     3781 gaactggcgg agaaaattgc gaccgatgcg ccgttttctc cccatgtctg tagaatcagg
     3841 ttttggggaa cgaccatggg cgctcccatc cggtttcatg gcattgaaac ggaaggaaaa
     3901 ataagacctc cccacgctga ggaattgccc ggcctgagct atctggccta tggaacgtcg
     3961 cttacccagg gagcttatgc ttcggaatcg catttgagct atccgaatct ggtcggctgc
     4021 cgtctcggcg tggacgttat caatctgggt tcttcctgct cggcatattg cgaaccggaa
     4081 atggccgact atatcgccgg aagaaaggac tggagctttg cgacgcttga tctttccctg
     4141 aacatggtcg acacattttc gccggaagaa ttttcgaggc gagtcagtta catgatcaac
     4201 aggatcgccg gcagcaacaa aagccgtccg gttttttgca tcacgctcaa gccgtatctg
     4261 ggagattata tcgaaagcaa aaagcccgac ctgtaccgca aattgcttcg gaaagccgtg
     4321 gaaaattctg cgctggataa tctctatctg atagaaggtc aggacctgat gccggatgtt
     4381 gcgggcggtc tttgcccgga catggtgcat ctgggagact tcgggacgat tcaaatagcc
     4441 gaaaaactgg ccgcaaaaat aaagccggtt ctgcaatctc atcatcttct ttttaaacac
     4501 tcccgatagg gattcgttga atttgccgcc ttcccccgtg tccggctttg ttgaaacggc
     4561 ttccgtttga taaagaatgt agacacgatg ctcataatac gttaaaaata cttatatttt
     4621 cattttgtca ttgagaacat tttgcattta ttgtaaggtg ccaatgaaag tgctggtttg
     4681 gggggtatta aatgtatgat cacgacactt ccaaatttca atccgtcaag gaattcataa
     4741 aacacaatta tcgacacttc aattccgctg tcgtcgtgga tgcagcggag gcctacgcga
     4801 atcatattaa taacggcggg aaaatgctga tggcactggc cggggccatg agcaccgcgg
     4861 aacttggtat cactctcgcg gcaatgattc gcagcggtaa aattcatgcc gtatgctgca
     4921 caggcgcaaa tctggaagag gatcttttca atctggtcgc ccacaagcat tacaagcgtg
     4981 ttccaaatta cagggatttg aaacccgagg atgaagcgga actgctgaga aacaagctga
     5041 accgggtgac ggacacctgc atccccgaag aagaagccat gcggaaggtc gaaggcaagc
     5101 ttttcagttt ctggaaaaaa gctgaggatg agaataaacg gtacttcccc tatgaattca
     5161 tcatgcagat catcagcagc ggcgtgttga aagatcagta tgaaatcgat gaaaaagatt
     5221 catggctggt tgccgcctgt gaaaaaaaca ttccgatttt tgttcccgga tgggaggact
     5281 cctctctggg caattatttt gtttccagtg tccgcaggaa acaacttaag cattattcga
     5341 ttgtgaaatc cggtctggaa acaatggatg ccttgattga ctggtattta gatgcatccc
     5401 ggcacagcga aacgggcttt ttccagatcg ggggaggtat tgccggagat ttttccattt
     5461 gtgttgttcc cctgattcag caggacatga aaatggactg ccggttatgg tcctatttct
     5521 gtcagatttc ggacagcacg acatcatacg gctcgtatag cggcgccgtt cctaatgaaa
     5581 aaatcacctg gggaaagatc cgccccgatt caaaaaaatt catcattgaa agcgatgcca
     5641 caattgttgc ccctctgatt ttcgcttacg ttcttgcgga ataagcggga ttcgcccggc
     5701 ggttttcaaa tttaaaatcc ggtgatgttc cgggcaggag aaaaaccgat ttcccgggca
     5761 taggtgaatt gccgttgcca gggaatgccg ggatggactt tttcgaaaat atctgcgccg
     5821 gtcattttga gcgattccgc atccacgggc acgggatgat aaccgccgat aaatcccata
     5881 tcctcatgaa tcaccggatt cttaccgggg agacagtcgc attccacggt aatattcagc
     5941 agtgaattga tgacgatggt cttgtccttg attattttcc acacggccgc cgccgtttcc
     6001 acaagctttt cctgaaaaac cgtcatgtcc gaattccaga aaatctgcag tgccatttcc
     6061 ggacacatcg caatacactg ggcgcagccg atgcattttt ttaaatcata tgtgggatag
     6121 tcggatgttg cgatagttgc cgctccggtc ggacagattt ccgcgcaaac tccgcagcgt
     6181 gtacattttt tcttattcag gacgggatga taatcggaat gcatgcgttg cttctgcgcg
     6241 cgggacgcga aacccataga catattttta atggcgcctc cgaaaagcga aaccatgtgt
     6301 cctttgaaat gggagaagat gacaaacccc tgcgcgttct caaaaacatt ggccacctgc
     6361 acggtcctga aatgattgaa cccggcggga atatccacaa gattgcggct gtccatgccg
     6421 tcggcgataa tcaccggaca acccagaaag tcatgcgtgt agccgtggga ggcggccgtt
     6481 atgagcgagt cttcggccgt acggcgtccg ccggaataga gaaccgttgt atcaaaaaca
     6541 aacggtttag ttccgtgggc aattagccag gtagcaattt cccttgcata atcaggcggc
     6601 agaaaacctt tattaccttt ttctccccag tgtagtttta tgccgacggt gtcgccggcg
     6661 gcgaagggag accggaggtg ttgaagaagt tttctcagcg attcaaggta aggttttttt
     6721 ccgcctttta ccggttcaaa gaataaatct ttcatggtcc gtctccgttc cttatttgga
     6781 taacatattt taaatctttt tcttttttat tgcatcaaaa aatcatcttc gagtctgttc
     6841 aaagaatcga taat
//
LOCUS       DAOSME010000095         6664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_12649_length_6664_cov_19.354365, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000095 DAOSME010000000
VERSION     DAOSME010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12649_length_6664_cov_19.354365"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..2108)
                     /locus_tag="PLG98_10405"
     CDS             complement(117..2108)
                     /locus_tag="PLG98_10405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOE33716.1"
                     /translation="MKKILVLILLGITIQAYAQLPPIIDRELFFGDPKISGAQISPDG
                     KFITFIKPFNNVPNIWVKERKQKFEEARPLTNDTKRPVRNYFWSRDSRYVLYVQDKGG
                     NENYRLYAVDPTVKGDPVPQTRDLTPMENVKAYIYDVPRKTPNEILIGLNDRHPQLHD
                     VYKLNLTTGEKTLIRKNDENVARWVFDLDAKLRLGSRQTQDGGTEILKVDGDKLVSIY
                     SVNNEETVSRYRFTVDGKSFYMISNKGSKADKTELLLYNLDDNTTKFIERDPENEVDF
                     GGALFSDVTNELLATYYIADHFKIYFKNKEFEKSYNQLKKAVPEGEIRFTSMTSDENL
                     WIVNVSRDVDPGSTYLFDRTTGKAELLYKSRPDLPTENLAQMKTIKYRAQDGIMIPAY
                     LVLPKGIAAKKLPLVIYPHGGPWERDYWGYNPTAQFLANRGYAVLLPNFRGSTGYGKK
                     FLNAGNKQWGRAMQQDLTAGVNYLIKEGIADPKKVAIAGASYGGYATLAGLAFTPDLY
                     AAGFDIVGPSNIVSLLKSIPPYMAPLKKAFAIRVGDMDNPEEKKMLDEQSPFNYANNI
                     KAPLYVVQGANDPRVNKAESDHIVVALRDLGRDVEYMVAPDEGHGFAGRENRLAMVVA
                     MEKFFSKHLGGRLQQDVKPEIQKKLDEITVDVKTVTIPK"
     gene            complement(2141..2371)
                     /locus_tag="PLG98_10410"
     CDS             complement(2141..2371)
                     /locus_tag="PLG98_10410"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE33717.1"
                     /translation="MPQRDHQYNMHSAYDTLRYGSSVEAQTAKYIEKVKILINSGIDV
                     NCKSKAGYTALHWAQRAGMGRIVDILKKAGAR"
     gene            complement(2346..3716)
                     /locus_tag="PLG98_10415"
     CDS             complement(2346..3716)
                     /locus_tag="PLG98_10415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE33718.1"
                     /translation="MTPKPTYKDLEKRIRILEAEIIKCQRIDELLRDNDLRFKKLSAH
                     IPGIVYQLMRRPDGTYCIPFTTDAITHFFGCLPQDVAEDITAITRVIFPDDLDNVLGL
                     IESSAASMTIYHCEFRVQIPGQPIRLMYGHATPEKMADGGIIWHGVIMDITDRKMIEE
                     ELRKSEEQNRALLDGMIDTVWVIDFDANLIDVNKAAVEQLGYSKEELLAIGLPGIDFS
                     LKPNDIKTLASTMPSDELQIFETSHTAKNGRTIPVEVCSSLVTYRGKRAILSIARNIT
                     ERKQMEEQIRHMANHDPLTALPTLRLANDRLDMAISEATRNNTMVAVLFLDLDDFKEV
                     NDTFGHSAGDSVLKQVALRMLDCVRKADTVARIGGDEFLVIASGIHAPKDAALVAEKI
                     IRVVPQPIMIDTKQIVVGVSIGIALFPDHSEDKEQLLELADEAMYMIKKSGKNGYRFA
                     TARSPV"
     gene            complement(3893..>4657)
                     /locus_tag="PLG98_10420"
     CDS             complement(3893..>4657)
                     /locus_tag="PLG98_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE33719.1"
                     /translation="IFEKKEKTAVIKLNRPKEFNALDYELTGDLARALEICKNERDIR
                     AIVITGVGKAFCSGGDVAMFGSAKDKGEAIRQLVELLHNVTVGIRQMPKPVIAAINGV
                     AAGGGLSMAAACDLRISASTAKFKQAYTSIGLAPDGAWSLTVPLLIGFGKATELAFLD
                     PTFDAKKALDLGLVHQVVDDAELEKVAMDVAGKLSRGPATAFAVVKDNLNRSMMGLLA
                     QQLELERRGMSRLGKTADAEEGITAFVERRKPLFST"
     assembly_gap    4658..4757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4823..6220)
                     /locus_tag="PLG98_10425"
     CDS             complement(4823..6220)
                     /locus_tag="PLG98_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOE33720.1"
                     /translation="MSKKQVIGSCLAVIVGIVVALITPPQGLTPPAMYGLGILLWAVT
                     CWMFEVAPDYIIAMAMCTLWVLFQCVPFKTAFATFSDPTWWLLLGAMGMGVAVSKSGL
                     LRRISLRVMRTFPATFNGQVLAMISAGTVIAPLVPSMTVKAAIVAPISMGISDAMGYP
                     RKSRGAGGLFGAMYLGFVLTGPMFISASFIGYMMRGLLPQDIQSQFTWTLWFTASLVW
                     SVVVLILMYIAIRFFYTPKEKDSLPPDYAAKQLAALGPMQRNEKITAAVLIMALLCWM
                     TEALHGIHATVIALSGLVVLLICKVFDRQDFRSGMGWDNMIFIGGIINLGSVLPVLKI
                     DKWIAQMASPVIGPMISNIYLFIVVLCFAIYAIRFILVSFTATTAIFTVLFVPFAQQA
                     GVNPWITGFIVYVSTGIWHVFYQNSTFLTSFYAVEGKMVTHRQMVPLSLAYSAISIAA
                     SLLSVPLWRYLGLVP"
     gene            6409..>6664
                     /locus_tag="PLG98_10430"
     CDS             6409..>6664
                     /locus_tag="PLG98_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOE33721.1"
                     /translation="MVAKNKSAAIKGKPFLALAFLSIVWGYNWVVMKEGLRFCDPFVF
                     AALRVALAAAFLLPLLMWSGKQLRPRQIKGTILLGLFSTTL"
BASE COUNT         1619 a         1666 c         1475 g         1804 t
ORIGIN      
        1 gtctcattcc tcaggattcg cgcgcctcgc ctgcggatct ttttacgatg ccgtctttat
       61 taatgtatta tctgaaagtg ttttataatt tttagtgtcg cacccaaatg tccagcttac
      121 ttaggaattg ttaccgtctt aacgtcaacg gttatttcat ccaatttctt ttgtatttct
      181 ggtttaacat cttgttgaag acgtccaccg agatgtttgg aaaagaattt ttccattgcc
      241 actaccattg ctaaacggtt ttccctacct gcaaacccgt gaccttcatc cggtgcaacc
      301 atatattcca catccctgcc aagatcacgc aaagcaacga caatatgatc cgattccgct
      361 ttatttacac ggggatcatt ggcaccctga acaacataca atggagcttt aatattattt
      421 gcataattga atggtgattg ttcatcgagc attttcttct cttcggggtt atccatatcg
      481 ccgactctta tggcaaaagc ctttttaagc ggcgccatgt acggaggaat tgattttagt
      541 agtgacacga tgtttgaagg accaacaata tcgaaaccgg ccgcgtataa atcgggagtg
      601 aatgctaaac ccgctaatgt tgcataacct ccgtacgaag ctccggcaat ggcaactttt
      661 ttaggatcag caattccttc tttgattaaa taattaacac cagcggttaa atcctgctgc
      721 attgctcttc cccactgttt attaccggca tttaggaatt tttttccgta cccggttgaa
      781 cctctgaagt taggaagaag aaccgcataa cctctgtttg ccaaaaattg cgcggtgggg
      841 ttatatcccc aataatcgcg ctcccaggga ccgccatgcg gataaataac caaaggtaat
      901 tttttcgcgg caattccttt gggcaagaca agataagcgg gaatcataat cccatcttgg
      961 gctctatatt ttattgtctt catctgggca agattttctg taggaagatc gggacgcgat
     1021 ttatatagaa gttcagcctt tcctgttgtt ctatcaaata aatacgtaga accgggatca
     1081 acatctctcg atacattaac gatccacaga ttttcatctg aggtcattga tgtaaatctg
     1141 atttcacctt caggaactgc cttctttaat tgattatagg atttttcaaa ttctttgttc
     1201 ttaaaataaa ttttaaaatg atccgctatg taatatgttg ccaacaattc attggtaaca
     1261 tcggaaaaaa gagctccgcc gaaatcaact tcattttcag gatcacgctc aataaatttt
     1321 gtggtattat catcaaggtt ataaaggagt aattctgttt tatcagcttt actgccctta
     1381 ttcgaaatca tataaaaact ttttccatcc actgtaaaac gataccgcga caccgtctct
     1441 tcgttgttaa cggaataaat agatacaagc ttatctccat caactttaag aatttctgtt
     1501 ccgccgtcct gggtctggcg tgaacccaat ctaagcttag cgtcaagatc aaaaacccat
     1561 ctggcaacat tctcatcgtt ttttcgaatt aaggtttttt ccccggtggt aagatttagt
     1621 ttataaacat catgaagctg gggatgtcga tcatttaatc cgataagaat ttcgttggga
     1681 gtttttctcg gtacatcata aatataagct ttaacatttt ccataggagt tagatctctg
     1741 gtttgaggaa cagggtctcc ttttaccgta ggatctactg catatagtct ataattttca
     1801 tttccaccct tatcttgaac ataaagaaca tatctgctat cccgagacca gaagtaattt
     1861 ctgaccggtc ttttagtatc gtttgtaagc ggtctcgctt cttcaaattt ctgctttctt
     1921 tcctttaccc aaatattagg cacattgtta aacggtttga taaaagtaat gaactttcca
     1981 tccggcgaaa tttgcgcacc ggatattttg ggatcgccaa aaaataattc acgatcaatt
     2041 attggtggta attgtgcata tgcctgtata gtaattccta ataaaattaa aactaaaatt
     2101 ttcttcataa tacgctccta acttttggta aaaatcaaaa ttaacgtgct ccggcttttt
     2161 taagaatatc cacgattcta cccattccgg cccgctgtgc ccaatgaagc gccgtatatc
     2221 cggcttttga tttacagttc acatcaatgc cgctgttgat aagaatttta accttttcaa
     2281 tatattttgc cgtctgagct tctacagacg aaccatacct cagggtatca taagccgaat
     2341 gcatgttata ctggtgatct cgctgtggca aagcggtatc cgttcttgcc tgattttttg
     2401 atcatataca tagcctcatc agcgagttcg agcagctgtt ccttatcttc gctgtggtcg
     2461 ggaaacaatg cgattccaat gcttacgcca acgactatct gtttcgtatc gatcatgatc
     2521 ggttggggca caacccggat tattttttca gcaaccagcg ccgcatcctt gggcgcatga
     2581 attccggatg caataaccaa aaactcatcc cctccgatcc gggcgactgt atccgctttc
     2641 cgtacgcaat caagcatgcg tagggcaacc tgcttgagaa cgctgtcacc ggcactatgg
     2701 ccgaaagtgt cattcacttc cttgaagtcg tccagatcga gaaacagtac ggccaccatg
     2761 gtattgtttc gggtcgcctc gcttatagcc atgtcaagac gatcattggc caatctcagc
     2821 gtgggcaaag ccgtcagagg atcatgattc gccatgtggc gtatctgctc ttccatctgt
     2881 ttgcgttcgg taatattcct ggcaatgctc aggatcgccc gcttcccccg gtacgtcaca
     2941 aggctggagc atacctcgac cggaatagtt ctgccatttt tggccgtatg ggatgtttca
     3001 aaaatctgca actcatcgga aggcatcgtg ctggcaagcg ttttgatgtc attgggtttg
     3061 agcgaaaaat cgatgccggg taaaccgatg gcaagcaact cctcttttga atagcccaac
     3121 tgttccacgg cggctttgtt cacatcgatg aggttcgcat caaagtcgat aacccaaacc
     3181 gtatcgatca tgccgtcaag cagagctcgg ttttgttctt cgctcttcct gagctcctct
     3241 tcgatcatct tgcggtccgt gatgtccatg atgacaccat gccagatgat gccgccgtca
     3301 gccatttttt cgggtgttgc atggccatac atcaatctga tcggctggcc gggaatctgc
     3361 actcgaaatt cacaatgata gatcgtcatg gatgcggcgg aagattcaat caagccaagg
     3421 acattatcca aatcgtccgg gaagatgacc ctggttatag cagtgatgtc ctcagccaca
     3481 tcttgaggca aacaaccaaa aaagtgcgtg atggcgtccg tggtgaacgg tatgcaataa
     3541 gtgccgtcag gccgcctcat caactgataa acgatcccgg ggatatgagc agagagtttt
     3601 ttgaaccgga ggtcattgtc cctcaggagc tcatcaatcc gctggcattt gatgatttct
     3661 gcttcgagta tcctgatcct cttttcaagg tccttgtagg taggttttgg ggtcatgggg
     3721 aagtggccct ccgatttcac cccgaggcgt aggatgaagg ttggtttctc cggttgccga
     3781 atgttttccc atcggattat ggcttaaatc ttaacatgtt tctcaaaatg aatcaatcga
     3841 accgttccgg caaccatttt ggaagcgatg taccagctga attttctccg ctctatgtgc
     3901 tgaacagggg ttttcttctc tccacaaagg cggtaatacc ctcttcggca tccgccgtct
     3961 tcccaaggcg gctcatgcct cttcgttcca gctcaagctg ctgtgccaac aatcccatca
     4021 tggacctgtt gagattgtct tttacaaccg caaaagcggt tgccggacct cgggaaagtt
     4081 tccctgccac atccattgcg actttttcca actccgcgtc atcgacaact tgatgaacca
     4141 gtcccaggtc caatgccttt ttcgcatcga atgttggatc cagaaaggcc agttccgtgg
     4201 ctttcccaaa gccaatcagc aagggaacag tcaaggacca agccccatcg ggtgccaagc
     4261 ctatggaggt ataagcttgc ttgaacttcg cagttgaagc gctgatcctt agatcgcagg
     4321 ctgcggccat gctcaatcca ccaccggcag ccacgccgtt gatggcagca ataaccggtt
     4381 tgggcatttg tctgattcct accgtgacat tatgcaacag ctccaccagt tgcctgattg
     4441 cctccccttt atccttggcg ctgccgaaca tggcgacatc tccgccggaa caaaaagcct
     4501 ttcctacccc ggtgataact atcgcgcgga tatctcgctc attcttgcag atctcaagcg
     4561 ctcttgcgag gtcgccggtt aattcataat ccagggcatt aaattctttt ggcctgttga
     4621 gcttgatcac ggctgtcttt tctttcttct cgaaaatnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnggt gtatctgact gcggcttcgc tcttcatcgt atggtccttt
     4801 gttgcgattt gaaaaaaatg gattatggca ccagacccag gtaccgccac agggggacac
     4861 tcagcagcga ggcggcaatc gatattgccg agtacgccag agacaggggc accatttgtc
     4921 tatgggtcac catcttcccc tccacggcat agaaagaggt aagaaacgtg ctgttctggt
     4981 aaaacacgtg ccagataccg gtcgagacgt agacgatgaa ccccgtgatc cagggattga
     5041 cgcccgcttg ctgggcgaag ggcacaaaga gaaccgtaaa gatcgccgtc gtggccgtga
     5101 aggataccag gatgaaccgg atcgcgtaga tcgcaaagca caggacgacg atgaagagat
     5161 agatgtttga aatcatcggc ccgatcacgg ggctcgccat ctgggcgatc catttgtcga
     5221 tcttcaatac gggcaagaca cttcccaggt tgatgatccc gccgataaat atcatattgt
     5281 cccaacccat tcccgagcgg aaatcctgcc ggtcaaaaac cttgcatatc agcaggacga
     5341 ctagccccga cagggcgatt acggtggcat ggatgccgtg gagcgcttcg gtcatccagc
     5401 acaggagggc catgatcagg actgctgcgg taattttttc gttgcgctgc atcgggccga
     5461 gagccgccaa ctgtttggct gcgtaatccg ggggaagtga atccttctct ttgggcgtgt
     5521 agaagaaccg gatggcgata tacatgagga tcagcacaac cacggaccag acaaggctgg
     5581 ccgtaaacca caaggtccaa gtaaactggc tctggatatc ttggggcagc agtccccgca
     5641 tcatgtaacc gataaaggac gcgctgataa acatgggacc cgtcaatacg aagccgagat
     5701 acatggcgcc gaacagcccg cctgccccgc gactctttcg tggataaccc attgcatcgc
     5761 tgatccccat actgatcgga gccacgatgg ccgccttgac cgtcatgctg ggaaccagcg
     5821 gagcgatgac ggttccggcg ctgatcatgg ccaggacctg gccgttgaag gtggccggga
     5881 atgttcgcat gacccgcagg gagatccgcc ggagcagacc gcttttcgac acggccactc
     5941 ccatgcccat cgcaccgagg agaagccacc aggtcgggtc ggaaaacgtc gcaaacgccg
     6001 ttttgaaggg aacacattga aacaggaccc ataatgtgca catcgccatg gcaatgatat
     6061 agtccggagc aacctcgaac atccaacacg tcaccgccca aagaaggatg cccaggccgt
     6121 acatggccgg cggggttaaa ccctggggag gcgtgatcaa cgctacaacg atgcccacaa
     6181 taaccgccaa acacgatccg atcacttgtt ttttcgacat cttcaaatac ctttaatatg
     6241 aggccacttc gttgaacagg cggagtattt cccctccaag tgtccattcc tgtagcgttg
     6301 ttccattgat ttgtccagat tgatcttagc gtttccggct tcatctattc acgatggatc
     6361 atcttcattt cttgacatta cgggtttgtt cttttatttt tgatcctcat ggtcgccaag
     6421 aataaatccg ccgccatcaa aggcaaaccc tttcttgccc ttgcttttct aagcattgtc
     6481 tggggctaca actgggtggt gatgaaggag gggctccgct tctgcgatcc tttcgtcttt
     6541 gccgcccttc gcgtggccct ggccgccgcc ttccttttgc cgctcctgat gtggtcaggg
     6601 aaacagctcc ggccccggca gatcaaaggg acgatcctcc tgggcctgtt ttccactacc
     6661 ctgg
//
LOCUS       DAOSME010000096         6611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_12767_length_6611_cov_20.793319, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000096 DAOSME010000000
VERSION     DAOSME010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12767_length_6611_cov_20.793319"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLG98_10435"
     CDS             <1..227
                     /locus_tag="PLG98_10435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOE33722.1"
                     /translation="TVSRARKIQRFLSQPFFVAAQFTGTEGKFVSVPDTIRGFKEILE
                     GKHDDLPEQAFYMVGGIEEAVEKAKKIGGE"
     gene            249..656
                     /locus_tag="PLG98_10440"
     CDS             249..656
                     /locus_tag="PLG98_10440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOE33723.1"
                     /translation="MADELLMEIVTPEKMIFSGNIEEVTLPGTEGEFGVLRGHEAFLT
                     SVEIGEMNYVKDGKKSYYAVNTGYVEVTGDKVTVLIETAEKSDQIDKERALKAKDNAE
                     SRLSQMNKDDVEFEIMRAALARAIARINVAEKQ"
     gene            complement(761..3742)
                     /locus_tag="PLG98_10445"
     CDS             complement(761..3742)
                     /locus_tag="PLG98_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOE33724.1"
                     /translation="MSEKSDFPEPTLREGDELYGFTVTRIKTFPTIHTTAYEMKHNKT
                     GAKLLHLHCFDRENLYAIGFRTPPYDSTGLPHILEHSVLAGSEKYPLKDVFKELMRST
                     MQTFINAFTYPDKTVYPVASQIKTDFYNLARVYTDLVLHPRMLRETFLQEGHHLEFSV
                     LDDLSSDLIISGIVYNEMKGAYSSPDSLIYKTLQENVYPDSVYSFDSGGDPNVIPSLT
                     YEQFCEFHSKYYSPSNARFFIYGDIPTKDHLVFLSEILKNFNYIDINSAIGSQKRFTQ
                     PRSVVGGYPIGKDESVERKTVVNIAWLMSENTDFETSLILQIISALLVGSAASPLRKA
                     LIDSGLGEDLTPVSGVEADLKQLMFCVGLRNTQRSDAQKIEQLILKTLKDIIDSGYDT
                     QLIEGVLHQVEFHGKEIVRGSYPYGISLMGAVFQTWLYDGDPLIGLDFSQIIENIRRC
                     WADDPLIFQKMTEKWLLNNPHRVLAVMEPDPDFNEKKETVYREKMDTLKASLCEEELR
                     QINAKAKKLKEFQNQQDPPEAASALPRLNMEDIPKSIEKTPTRNSLIGDVPVWEHDLF
                     TNGIAYVDLAFDLADIPEELQIYLPLLGKMMTGMGAAGLTYEEMAKRIALKMGGLGYN
                     LGAGFTANGDSTWQKMIFSFKALYRNLPEAMSIISDVLCRGDLSHEARMRDLISERKN
                     NLQSAILPSGHLFARMAAGAALTLPAYRDEQWHGRTQFKFVQATAGEFVSSKSALRDK
                     LETLKQTLFNKNNMVINVTAEHQGLEIAKEHLPCLLDRLPASSQAAPEEEPSLNPVFA
                     GISVPAQVSYVAYILKTPCYTDPSSPLLMIAAKELSNNYLYKYIRVQGGAYGGMSSFD
                     ASMGLFSFLSYRDPRISETLQVFKNAQTFYSQNEISSPDMEKAIISTISMIDKPSDPA
                     SKGYTAMMRNFAGITDKIRQKFRDDILSATPQKLKKVLSGYFSQAFKSASVAVYSSPE
                     KLDEANRQLEDKLHIESIFEN"
     gene            complement(3809..4651)
                     /locus_tag="PLG98_10450"
     CDS             complement(3809..4651)
                     /locus_tag="PLG98_10450"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOE33725.1"
                     /translation="MPERKSILCPNCRKLISMDEPSCPYCGLQRPGMHNIAGNIGKLF
                     MASNPVMIIIYVNVALYIFSIFLDPQGIFGGSGPFGILAPSNQSLFLLGASGTFPVIG
                     AHRFWTLISASFLHGGIFHILFNMMALYQLGPFIIREFGFHRFINLYIVTGICGFSAS
                     VISGTPFTIGASAAICGLIGAIIYYGKSRGDSYGEAIYKQAMGWVIALVIFGLIFRGI
                     DNWAHAGGLLSGIGFSFLMGYNDNKQETAWNKMLAYACILLTAAVLLWSVVNSLFIGL
                     NISI"
     gene            4802..5974
                     /locus_tag="PLG98_10455"
     CDS             4802..5974
                     /locus_tag="PLG98_10455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOE33726.1"
                     /translation="MKNRSVIKIESVAFGGSGVGRADNLVVFVPFSAPGDELEIEITQ
                     RKKNFLRGRIRNIIKPSPWRTDPVCRYYGVCGGCCYQHIHYAYQLAIKKKQVEDAFRR
                     IGKVDAPPVLETVASPRMYHYRGKARYHVGDDPQTGHIGFLDISGGKIVDIEHCAIMQ
                     ETINEQLSILRENRQNLWHKDELTIWSRYPDVTSEDQTSVVRIVQGKRILAPDDGFFQ
                     ANEYLTDRLVDEVCRLVRPGKAENAIDAFCGSGLFSIFLSPFAEDITGIEMDKESVKY
                     AKINAANLNARNVRFLDGNVEIVLREKFLSLGSKIDLIVLDPPRVGCEMTLLKTLMDL
                     QPSNIIYISCNPATQARDIKYLCEGGYHLQSLHPLDMFPQTEHIEVIGYLKRIMAL"
     gene            6077..6553
                     /locus_tag="PLG98_10460"
     CDS             6077..6553
                     /locus_tag="PLG98_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33727.1"
                     /translation="MASKSKDVRIEQLRIFEKKLAVRLQQLEQKGISPEKAQNDAIVK
                     SLKAKIKQTKARIATFDKFVQQTQALAQAKAAKQAAVAEEVKASAEEAKPKAKKKEDG
                     AAKEPKKQPAAEDGAAKKAKKQPADGQEAAPKKAKKQPDEGDDAAPKKKATKKKEE"
BASE COUNT         1891 a         1423 c         1626 g         1671 t
ORIGIN      
        1 tgacggttag ccgcgccaga aagattcaga gattcctgtc ccagccgttc ttcgtcgccg
       61 ctcagttcac cggtacggaa ggcaagtttg tttccgtgcc cgataccatc agaggcttca
      121 aagaaatcct ggaaggcaaa catgacgatc tgcccgaaca ggccttttac atggtcggcg
      181 gcattgaaga agccgttgaa aaagccaaaa agattggcgg cgaataatta ttgatctgga
      241 gaatgtaaat ggcagatgaa ttattgatgg aaattgtaac gccggaaaag atgatttttt
      301 cgggcaatat cgaagaagtt acccttccgg gaacggaagg cgagttcggc gtattgcgcg
      361 gtcatgaggc ttttttaacg tccgtggaaa tcggcgagat gaattatgtc aaggacggca
      421 aaaagtcgta ttatgcggtg aataccggtt atgtggaagt tacaggcgat aaagtcaccg
      481 ttctgattga aaccgctgaa aaatcagatc agattgataa agaacgggcc cttaaggcca
      541 aagataatgc tgaaagccgt ttatctcaga tgaacaaaga tgacgtggaa tttgaaatta
      601 tgcgggcggc actggcgaga gccatcgcga gaatcaatgt agcagaaaag caataaaaaa
      661 atgcgataag gattttgttg cgattgcgat ttaaaatccc agaagaacat gaaagaggct
      721 aaagtatatt tagcctcttt ttttatgaag agctattgga ttaattttca aaaatacttt
      781 caatatgcag tttgtcttcc aattgtctgt tggcttcgtc cagtttttcc ggggaggaat
      841 aaacggccac tgacgctgat ttgaacgcct gagagaaata accggataat acttttttca
      901 gtttttgcgg tgtggcggat aaaatgtcgt ctctgaattt ctggcgaatc ttatcggtta
      961 ttccggcaaa attgcgcatc attgccgtat accctttgct ggccggatcc gaaggtttgt
     1021 caatcatgct gatggtgctg atgatcgcct tttccatgtc cggtgaagaa atttcattct
     1081 gactgtaaaa cgtctgtgcg tttttaaaca cctgaagcgt ctcggaaatg cgcgggtcgc
     1141 gataggacaa aaacgaaaac aatcccatgg aagcgtcgaa agaagacatg ccgccgtaag
     1201 cgcctccctg aacgcggata tacttgtaca gatagttatt ggataactct tttgcggcaa
     1261 tcatcaaaag agggcttgac gggtcggtat aacaaggcgt ttttaaaatg taggcgacat
     1321 acgatacctg tgcggggaca gagattcctg caaataccgg attcaaagaa ggttcttctt
     1381 ccggagcggc ctgtgaagac gccggaagcc tgtcgagtag acacggcagg tgttctttgg
     1441 caatttccaa cccctgatgc tcggcggtga cattaatgac catgttgttt ttattgaaca
     1501 gggtttgctt taaagtttcc agtttatcgc gcaaggcact ttttgatgaa acgaactcac
     1561 ccgctgttgc ttgaacgaat ttaaattgcg ttcgtccgtg ccattgttca tcccggtaag
     1621 ccgggagggt gagtgccgcg ccggctgcca ttctggcgaa tagatgaccg gagggaagaa
     1681 tcgctgattg aagattgttt tttctttctg aaatcagatc ccgcatccgg gcttcgtgac
     1741 ttaaatcacc gcggcagagc acatcgctga tgatgctcat ggcttccgga agattgcgat
     1801 atagcgcctt aaacgagaaa atcatcttct gccatgttga gtcgccattt gcggtaaatc
     1861 ctgcgcccag attatagccc aacccgccca tcttcagagc aatccgtttg gccatttcct
     1921 cataggtcaa accggcagcg cccatgccgg tcatcatctt tcccaaaagc ggcagataga
     1981 tctgcagctc ttcagggata tctgccagat caaaagccag atcaacatag gcaattccgt
     2041 tggtaaacag atcgtgctcc cagaccggaa cgtcaccgat caaagaattt cgggtcggcg
     2101 ttttttcaat ggatttcgga atatcctcca tgttcagcct gggcagggct gaggccgctt
     2161 ccggcggatc ctgttgattt tgaaattcct tgagcttctt ggcttttgca ttaatctgtc
     2221 ttaattcttc ttcacaaaga gacgccttga gcgtgtccat tttttctctg tacacagtct
     2281 ctttcttttc gttgaaatcg ggatcgggtt ccataacggc cagaacgcga tggggattat
     2341 tcaaaagcca tttttcagtc attttctgga aaatcagggg atcatcagcc cagcagcggc
     2401 ggatattctc tatgatttgt gaaaaatcca gtccgattaa cggatcgccg tcataaagcc
     2461 atgtttgaaa aaccgcaccc atcagagaaa ttccgtaagg ataagaaccg cgaacaattt
     2521 ccttgccgtg aaattcgacc tgatgcagaa cgccttcgat gagttgcgtg tcgtaaccgg
     2581 aatcaataat gtcttttaaa gtctttagaa tcagttgttc gattttttgt gcgtccgacc
     2641 gctgtgtgtt tctcaacccg acacagaaca ttaattgttt caggtctgct tcgacgccgc
     2701 tcaccggagt cagatcctct cccagaccgg agtcaatcag cgctttgcgc agcggactgg
     2761 ccgcgctgcc aacgagaaga gcggatatga tttgcaggat caatgaggtc tcaaaatcgg
     2821 tattttcaga catcagccaa gcgatgttaa caacggtttt acgttcaaca gattcatctt
     2881 ttccgatggg ataccctccc acaaccgaac gcggctgggt gaaacgcttc tggcttccga
     2941 tggcggaatt tatatctatg taattgaaat tctttagtat ttcagaaaga aacaccagat
     3001 ggtccttcgt gggaatgtca ccgtaaataa aaaaccttgc attggagggt gagtaatact
     3061 tgctgtggaa ttcgcaaaat tgttcatagg tcagcgaggg gataacgttc ggatccccac
     3121 cggaatcaaa tgagtaaaca ctgtcggggt agacattttc ctgaagcgtc ttatagatca
     3181 gagaatcggg ggaggaatag gcgcctttca tttcattata aactattccg gaaatgatca
     3241 gatcactgga cagatcatcc aaaacagaaa attccagatg atggccttcc tgaagaaaag
     3301 tctccctgag catccgcgga tgcagaacca gatcggtata aacgcgggcc agattgtaaa
     3361 aatcggtttt tatctgactg gctaccggat aaacggtttt atccggatag gtaaacgcgt
     3421 tgatgaatgt ttgcatggtt gagcgcatta actccttgaa aacgtctttt aagggatatt
     3481 tttcagagcc ggccagcacc gagtgctcca gaatatgcgg caggccggtg gaatcgtacg
     3541 gaggcgtgcg gaatcctatc gcgtataaat tttcgcggtc gaagcagtgc agatgcagta
     3601 gcttggcgcc ggttttattg tgtttcattt cataagctgt cgtatgaatc gtggggaagg
     3661 tttttatgcg tgtaaccgtg aagccgtaaa gctcatctcc ttcacgtaat gtcggttctg
     3721 gaaaatctga tttctctgac attcaattaa actcctcttg aatagatata tggcgaagcc
     3781 gtcttcaaaa aattagccgc cagcatgttc agatggatat gttcaggccg atgaaaagcg
     3841 agttgacaac cgaccataat aaaactgcgg ccgtgagcag gatacaggcg taagccagca
     3901 ttttgttcca tgcggtttcc tgcttattgt cattgtaccc catcagaaac gaaaaaccga
     3961 tacccgagag cagtccgccg gcatgagccc agttgtcgat acctctgaaa atcaggccga
     4021 aaatgaccag ggcgatgacc cagcccatgg cctgcttgta aatggcttct ccgtaggaat
     4081 cgccgcggct tttcccgtag taaatgattg caccgatcag accgcagatc gctgcagaag
     4141 ctccgatcgt gaatggcgtt cctgaaatca ctgaagcgga aaatccacat atgccggtaa
     4201 cgatatacag attgataaac cggtgaaacc cgaattcccg gataataaac ggtcccagct
     4261 gatacaatgc catcatattg aacagaatgt gaaaaattcc gccatgtaga aaagaagccg
     4321 aaatcagggt ccagaaacgg tgggctccga taacgggaaa ggtgccgctt gcacccagca
     4381 gaaaaagact ttgattggac ggtgccagta tcccgaaagg accgctgccg ccaaaaatgc
     4441 cctgcgggtc cagaaatatc gaaaaaatat acaaagcaac attaacgtaa atgatgatca
     4501 tgaccggatt ggacgccata aaaagttttc caatattgcc ggcgatattg tgcatcccgg
     4561 gacgttgaag tccgcaatag gggcaggagg gttcatccat gctgatcaat ttacggcaat
     4621 tgggacagag tattgatttt cgttccggca tttttatcct tttttaaaaa aaattgttct
     4681 gtaaccgttt caggactgtg tttaattaac aacattatta tgagttgtaa agaaaaatac
     4741 catcatttat tgacgattga tttattgatg tgttacgtca gacacggagc attgttttga
     4801 aatgaaaaat cgatctgtga taaaaattga atcggtggct tttggcgggt cgggagtcgg
     4861 tcgtgcggat aaccttgtcg tttttgttcc tttttcagcg cccggtgatg aactggaaat
     4921 cgaaatcacc caacggaaga agaactttct gcgcggccga atccggaaca tcatcaagcc
     4981 gtcgccctgg cggacggatc ccgtctgccg ttattatggc gtatgcggcg gctgctgtta
     5041 tcagcacatc cattatgcgt atcaactggc catcaagaaa aaacaggtgg aagatgcttt
     5101 tcggagaatc ggcaaagttg acgcgccgcc tgttttggaa accgtagctt ctccacgaat
     5161 gtatcattat cgcggcaagg cccggtatca tgtaggagac gatccccaaa cagggcacat
     5221 cggatttctg gatatatccg gcggaaaaat tgtcgacatt gagcattgcg caatcatgca
     5281 ggaaaccatt aatgagcaat tgagtatctt gcgggagaat cgacaaaatc tgtggcataa
     5341 agatgagctg acaatctggt cccgatatcc ggatgttacc tctgaagatc agacgtcggt
     5401 cgtccgtatt gtccagggaa aaaggattct ggcgcctgat gacgggtttt tccaggcaaa
     5461 tgaatatctg acggatcggc tggtggatga agtttgtcga ctggtaagac ctggaaaagc
     5521 agaaaacgca attgacgcct tttgcggaag cggccttttc tccatttttc tttcaccttt
     5581 tgccgaggac attaccggga tagaaatgga caaggaatcc gtaaaatacg caaaaataaa
     5641 tgctgcaaat ctgaatgccc gaaatgtccg ttttcttgac gggaatgttg aaattgtatt
     5701 gcgggaaaaa ttcctgtcct tgggcagtaa aattgatctg atcgttttgg atcccccgcg
     5761 tgtcggttgt gaaatgacgt tgttgaaaac tctaatggat ttgcaaccgt cgaacattat
     5821 ttatatttca tgtaatccgg ctacgcaggc gcgggatatc aaatacttgt gtgaaggcgg
     5881 atatcatttg caaagtcttc atccgttgga catgtttccg cagacagaac atattgaagt
     5941 aatcgggtac ctaaaacgca tcatggcatt gtaacttttt ttgggggaaa tcggcccgtc
     6001 gatgacaata atatttatag atttaaataa aaaaaattgt tattaaaaac cacctaaata
     6061 aacaaggaga gggaagatgg catcaaaaag caaagatgta agaattgaac aactccggat
     6121 tttcgaaaag aaactggctg tgcgtttgca gcagcttgag cagaagggaa tcagccctga
     6181 aaaagcccag aatgacgcaa tcgttaaaag cctgaaagca aaaatcaaac agaccaaggc
     6241 aagaatcgca acattcgata aatttgtcca acagacgcag gcattggccc aggcaaaagc
     6301 ggccaaacag gctgctgtcg ctgaagaagt caaggcgtct gctgaggaag caaagccgaa
     6361 agcgaagaag aaagaggacg gtgccgcaaa agaacccaaa aagcagcctg ctgcagaaga
     6421 tggcgctgcc aagaaggcca aaaaacagcc cgctgacgga caggaggcgg caccgaaaaa
     6481 ggcaaagaag cagccagacg aaggcgatga cgccgctccg aaaaagaaag caaccaagaa
     6541 aaaagaagaa tagcgttgaa attgaaatca agaaaaaagg gggaagctgg aaaacttccc
     6601 cctttttatt t
//
LOCUS       DAOSME010000097         6465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_13107_length_6465_cov_14.744306, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000097 DAOSME010000000
VERSION     DAOSME010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13107_length_6465_cov_14.744306"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..916
                     /locus_tag="PLG98_10465"
     CDS             467..916
                     /locus_tag="PLG98_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOE33728.1"
                     /translation="MKRILILCLAMMFLLPCIAMPEESPVGKTKTSKGSVFVVRDGRQ
                     MPVAVGDRLFQNDTIRTGASSSVGIIFEDNTILSLGPDSEIVIDQYVFAPEKGVFSMI
                     ARMVRGTASYLSGIIGHQSPESVKFRTPEATIGIRGTHFLVKVDGCP"
     gene            996..1598
                     /locus_tag="PLG98_10470"
     CDS             996..1598
                     /locus_tag="PLG98_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE33729.1"
                     /translation="MKQLIGTVFCLGLVLVLAGCAVRSTVVLIPDPDGHVGQLVVANE
                     GGQQILNEKNQSVRVADLKTAPGKVKTMSAEEIQDTFADALAAEPLPPVKFILHFMPD
                     SDQLTDESRAVLPDIFQTIQSRQSTDIVISGHTDTVGEKNYNYTLSLERAQAIFKILE
                     AQGAVPAHITVTSHGEGNPLIKTADEVAEPKNRRVEVTIR"
     gene            1621..3675
                     /locus_tag="PLG98_10475"
     CDS             1621..3675
                     /locus_tag="PLG98_10475"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOE33730.1"
                     /translation="MKQASDKNQTDPAITTSKPERRRILIWGILCTLCFAVIFAFKPN
                     LMQRLDYLEYDFLLAKFPNNNASSKLVIVDLDEKSLSRYGQWPWPRYRVAGLLDKVIA
                     MQPDIISLDIVFAEPDRTSAGPLLKDLGDVYRIKIDLHGLPRSLKDNDQILAQTLAGG
                     PFVLGTIFHFDELKKGSDRCVLHPVKSSYINDDVNQNAHTEIPVSTGVLCNLPMLSDK
                     VSSSGFFNFSPDSDGVLRRLPMLIQYHGQLYPNLALATVLKLKNENHVILKKDRNHLQ
                     SIHYRGNAVPVDRHGQMLIKFRSSAQKYEYVSAADILDNNVSPERLRGRIVFVGTSAV
                     GLKELLNTPLGPVFPGVEVHATAVDNLLKGDFISLPGWSNGFILLLVMVSGLAMSLLI
                     SYQKAATGFVAMLLFAGGLWMTTHQVFFRMGFFVATAFPIASVICNYMFLTALKYRME
                     EKKNLAHMQELLLTQDITIESLANLTEHRHQETGGHIKRTRLYVRLLADHMKHRDKYR
                     TFLNDENIEMLDKSAPLHDIGKVGIPDKILLKPGKLTGEEFEIIKTHTTNGRNLIRSS
                     VRQFGKISFLNIAEEIAYTHHEKWDGSGYPQGLKGDAIPISGRLMALADVYDALISKR
                     VYKEAYPHAEAVEIIVNAKGKHFDPDMVDAFLEIHEQFRNIAREHADSQEAKESPKPD
                     AA"
     gene            complement(3760..4752)
                     /locus_tag="PLG98_10480"
     CDS             complement(3760..4752)
                     /locus_tag="PLG98_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33731.1"
                     /translation="MLKVVADQKAIKKYAGLFNKKFKPFVAATIKVKLGHQGASFSAK
                     VLWCEELGIWKFSRAVNDIRYWNAFGIGKPGTAGVLSIASEINFPWAQIDRKTGGAFA
                     EDAWGNLFVIHRGKIGGGKKGVGKSLFEQNYRGVWSFMEDGDSLSEVAVIGNLKSERF
                     AAQTALFVKKIEMMKLSAAESAQTEMDFAEISFREDLIGNESPLPEDEIADACDHDLV
                     VSKLAVHLQRQKIKIGNDTESELFAVDVSGNRISHIFEIVADAKEKNVMAAAGRLMLQ
                     TSAGALNPLPVLVLPDDIMNHYKSDLRRIHICVIGFHWQDTQAVFSGLEKISIR"
     gene            complement(4803..5924)
                     /locus_tag="PLG98_10485"
     CDS             complement(4803..5924)
                     /locus_tag="PLG98_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE33732.1"
                     /translation="MNWLRIRELVRKEFIQLFSDKRNRALMVVFPFIMMLVFGYVVNY
                     DIRNVRMAVLDYSKTHESRSLIKSFEGSNIFHVTHNVIHDRQMTELLLRQKIDMGIKI
                     SHDFASVIRAGQTAPVQLLVDGSMSNMTSIRVAYTATILEKFNREELRRLYPMDMRYG
                     RVDARIRTWYNPNLDSQYFFVPGIVAFLIMLTSFIFTSIAIIREKEDGTMEQLIVTPI
                     KSHEFIIGKTIPYTIIALFQLFAVTGVAVYWFEIPFKGSFLLLLLAACLFLLSTLGIG
                     LFISTISSTKQQAMMTAFFFVLPFFMLSGFVFPISNMPDVVQWLTYLNPLRFFLVIIR
                     DIFLKGVGFDVLWPQYLALTILGAVVFTGAIARSKKRLD"
     gene            complement(5921..>6465)
                     /locus_tag="PLG98_10490"
     CDS             complement(5921..>6465)
                     /locus_tag="PLG98_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE33733.1"
                     /translation="LLTSLVIAREYENGTMETLKSLPVRAGELLLGKALPYFFIGLTD
                     VLIAILLSQLLFGIVMKGSFWLMILSSSLYLGVALSLGLLISNVTQSQLLANQIAIMA
                     TYLPSFLLSDFVFPIVNMPAVIQAITFITPARYFIDILSGIYLRNLGFAYLWPSMLVL
                     LLMFIVLTSMNYRLLRKEGL"
BASE COUNT         1686 a         1625 c         1627 g         1527 t
ORIGIN      
        1 gattctgatt tcgtttcttg agagttgata ctataccgcg ccccggcctc ctatgtagaa
       61 gctaattata atattgataa taatgatggc acgaatatct ttcttcatca aaaatctctt
      121 gatatcaatt ttaactgcat cataattata ccccatgatt ttgtcaatag aaatgttgtt
      181 tcaggtgttt aatgaatggt tgcaaaaaaa ttgccggcat atttgatttt tacgggaccg
      241 ggaaaacagc ttctccttac gtccgatgaa aaccgataac tttattaatg caaacagtta
      301 tcggtctata gggattaaag attgttcttt tttaaggggg gctgttatga tatacataat
      361 ttgtaaaaaa acacaccttc catcgaagga aaaagagaca tccggagggc gatcctccga
      421 atgtgatacg ggtgccccgc aacgtcaacc ataagggagt ctacctatga aaagaatctt
      481 gatcctttgt cttgccatga tgtttctgct gccatgcatc gccatgccgg aggaatcgcc
      541 tgtaggaaaa accaaaacaa gcaaaggctc tgtttttgtc gtccgcgacg gcaggcaaat
      601 gcccgttgcc gtcggcgaca ggcttttcca aaatgacacc atacgcacgg gcgcgtcaag
      661 ctctgtggga atcatttttg aagacaatac tatcttgtct ttggggccgg acagcgaaat
      721 cgtgattgac caatatgtgt ttgcccccga aaaaggggtg ttttccatga ttgcgcgaat
      781 ggtgagaggg accgcgtctt atctttcagg cattatcggt caccagtctc cggaatcggt
      841 caagttccgg acgcctgaag cgaccatcgg tatccgcggc acacatttcc tggttaaggt
      901 ggatggttgc ccgtaacgca tggtgatacg attttcaatc acacgctttt cgccggacgg
      961 cgatttgccg gacatttctg atgatggagg cggacatgaa gcaattaatc ggaacagtat
     1021 tctgtttggg gcttgtcctt gttttggcgg gctgcgcggt ccggagcacg gttgtattaa
     1081 ttccggatcc tgacggccat gtcggacaat tggtggtggc caacgagggc ggacagcaga
     1141 ttttgaacga aaaaaatcaa tcggtcaggg tcgcggattt gaaaacagcg ccgggtaagg
     1201 taaagaccat gagcgccgag gaaatccagg acacttttgc cgacgctctg gctgccgaac
     1261 ctctgccgcc ggtgaaattc atcctgcatt ttatgccgga ttcggaccag ctgaccgatg
     1321 agtcccgggc ggttctgccg gacatttttc agaccataca aagcaggcaa tcgacagata
     1381 ttgtgatttc aggccatacc gatacggtcg gcgaaaaaaa ctacaactac accttgtcgc
     1441 tggaaagggc tcaggctatt tttaaaatcc tcgaggccca gggcgccgtg cctgcccaca
     1501 tcaccgtgac ctcgcacggg gaaggaaatc ccctgattaa aaccgctgat gaagttgcgg
     1561 aacctaaaaa ccggagagtc gaggtcacca tccgataaac agcattcgca ctgatcattc
     1621 atgaagcaag catcggacaa aaatcaaacg gaccccgcga tcacaaccag caaaccggaa
     1681 agacggcgta ttctgatctg ggggatcctc tgcaccctgt gctttgcggt catctttgcc
     1741 ttcaaaccga atttgatgca gcggcttgat tatctggagt acgattttct tctggcaaaa
     1801 tttccgaaca ataacgccag cagcaaactg gtcatcgtgg acctcgatga aaaaagtctc
     1861 agccgctacg ggcaatggcc ctggccccgg taccgcgtgg ccggactgct ggacaaggtg
     1921 atcgccatgc aaccggacat catcagcctc gacattgttt ttgccgagcc ggacagaacg
     1981 tcagcgggac cgctgctgaa agatctgggc gacgtctaca ggattaaaat tgatcttcac
     2041 ggactgcccc ggagtctgaa ggacaacgat cagattcttg ctcagactct ggccggaggg
     2101 ccctttgtcc tcggcacgat ctttcacttc gacgagctga agaaagggtc ggatcgctgt
     2161 gttctgcatc ccgtcaaatc gtcatacata aatgatgacg tgaatcaaaa cgcccatacg
     2221 gaaattccgg tgagtacggg cgtgctgtgc aatttgccca tgctgtcgga caaggtcagc
     2281 tcgtcgggct ttttcaactt cagtcctgat agtgacggcg tgctgcggcg tctgcccatg
     2341 ctgattcagt atcacggaca gctttatccc aatctggccc tcgccaccgt gctgaaactt
     2401 aaaaacgaaa accatgtcat tttgaaaaaa gaccgcaatc atctgcaatc catccattac
     2461 cggggcaacg ccgtgcccgt tgaccggcac ggccagatgc tgatcaagtt ccggagttcc
     2521 gcgcaaaaat acgagtacgt atcggcagcg gatattctgg ataacaacgt gtcgccggaa
     2581 cggttgcggg gccggatcgt gtttgtcggc acatcggcgg tcggcctgaa agaattattg
     2641 aacaccccgc tgggtcccgt attcccgggg gtcgaggtgc atgccacggc tgtcgacaat
     2701 cttctgaaag gggatttcat ctccctgccc ggttggtcca atggctttat cctcctgctg
     2761 gttatggtgt cggggttggc catgagcctg ttgatcagct accagaaagc cgccaccggt
     2821 tttgttgcca tgctgctttt tgccggcgga ttatggatga cgacccatca ggttttcttc
     2881 cggatgggtt tttttgtggc caccgcgttt cccattgcgt cggttatctg caactacatg
     2941 tttctgaccg cattaaaata ccggatggag gagaaaaaaa atctggccca tatgcaggaa
     3001 ctgcttctga cacaggatat caccatcgag agcctggcca atttgacgga acaccgccat
     3061 caggaaacgg gcggccatat caagcgcacg cgactttatg tcaggctgct ggccgatcac
     3121 atgaagcacc gggataagta cagaactttt ctcaatgacg agaacattga gatgctcgat
     3181 aaatcagctc ccctgcacga tatcggcaag gtcgggattc ccgataaaat tttgctcaag
     3241 ccgggcaaat tgacgggcga agagtttgaa attatcaaga cacacaccac caatggccgc
     3301 aacctgatcc gatcctccgt ccggcaattc ggtaaaattt cattcttaaa cattgccgaa
     3361 gagatcgcct atacgcacca tgaaaaatgg gacggctccg gctatcctca gggcctcaaa
     3421 ggggatgcca ttcccatcag cggaagactg atggcgctgg cggatgtcta tgatgccctg
     3481 atcagcaaaa gggtctataa agaagcctat ccgcatgccg aggctgtgga gattatcgtc
     3541 aacgcaaaag ggaagcactt tgatcccgac atggtggacg cgtttctgga aattcatgaa
     3601 cagttcagaa acatcgcccg tgaacatgcg gattcgcagg aggcaaagga gtcccccaag
     3661 ccggatgcgg cgtaaaacat ctgacgcagg ggcattatct tttccgcgtt agacctcaat
     3721 ccaattcatt ttaaaaaatc aacatgccgg acgacgatct tagcggatgc tgattttctc
     3781 cagtccggaa aaaaccgcct gtgtgtcctg ccagtgaaaa ccgataacgc aaatgtggat
     3841 tcgtctcaaa tccgatttgt aatgattcat gatatcgtcc ggcaacacca aaacgggaag
     3901 agggttcaac gcgcctgccg atgtctgcaa cattaatcga ccggcggctg ccatcacgtt
     3961 tttttctttg gcgtcggcga caatttcgaa aatatgagaa attctgtttc ccgatacatc
     4021 aacggcaaaa agttcacttt ccgtatcatt gccgatcttt attttctgcc gctgcaggtg
     4081 gacggcaagt ttgctgacaa cgaggtcgtg atcgcaggcg tcagcgattt catcttccgg
     4141 cagagggctc tcgtttccga tcaggtcttc ccgaaaactg atttccgcaa aatccatttc
     4201 cgtttgcgcc gactcggcgg cggacaattt catcatttct atttttttaa cgaacagagc
     4261 cgtctgcgca gcgaaacgct ctgatttgag attgccgatc accgccactt cactcaggct
     4321 gtcaccatct tccatgaagg accagacgcc gcggtaattt tgttcgaaca gcgatttgcc
     4381 gacgcctttt ttcccgccgc cgatcttccc gcggtgaatg acaaacagat tgccccaggc
     4441 atcctcggca aaagcgcctc cggttttcct gtcgatctgc gcccagggga aattgatttc
     4501 ggaggcgatg gaaagaacgc cggcggttcc gggtttgccg atgccgaaag cgttccagta
     4561 gcggatatcg ttgacggccc gggaaaattt ccagatgccc agttcttcgc accaaagaac
     4621 tttggcggaa aaactggcac cctgatgacc aagcttgacc ttgatcgttg cggcgacaaa
     4681 cggtttgaac tttttattga aaagtccggc atattttttg atggcttttt gatccgctac
     4741 gaccttgagc atgtgtccat ttcctgagat gttatatttt aataatatat gagaacgctt
     4801 cactaatcca gccgtttttt agaccgggcg atggcaccgg taaaaacaac tgcccccagg
     4861 atggtcaggg cgaggtattg cggccagagc acatcaaagc caacgccttt cagaaaaata
     4921 tccctgatga tgaccagaaa aaatctcagc ggattaagat acgtaagcca ctgtacgacg
     4981 tcaggcatgt tggaaatggg aaagacgaaa ccgctgagca tgaagaacgg cagcacgaaa
     5041 aagaaagccg tcatcatggc ctgctgtttg gtcgaggata tggtggagat gaagagtccg
     5101 atgcccagcg tgctcagcaa aaacaaacag gcggccagca aaagaagcag gaaacttcct
     5161 ttaaagggaa tctcaaacca gtaaacggcg acaccggtta cggcaaacaa ctgaaaaagc
     5221 gcaatgatcg tatagggaat ggttttgccg ataataaatt cgtgtgattt tatgggggtc
     5281 acaatcaatt gctccatggt gccgtcttcc ttctcccgga tgatggcgat ggacgtaaag
     5341 ataaaactcg tcaacatgat cagaaaagcg acgatgccgg gaacaaaaaa atactgactg
     5401 tcaagatttg gattgtacca ggttcggatg cgtgcatcca cccgcccgta acgcatatcc
     5461 atgggataaa gcctgcgcaa ttcttcgcgg ttgaatttct ccagaatcgt cgcggtatac
     5521 gccacccgga tggacgtcat attgctcatc gaaccgtcca caagcaactg aaccggcgcc
     5581 gtttgtcccg cgcggatcac cgatgcaaaa tcatggctga tcttgattcc catatcgatt
     5641 ttttgccgga ggagaagttc cgtcatctgc cggtcatgga tgacgttgtg cgtcacgtgg
     5701 aagatgttgc tgccttcaaa acttttaatc agcgaacggc tctcatgggt tttggaataa
     5761 tccagcaccg ccatccggac gttccggatg tcgtaattga cgacgtaacc gaacaccagc
     5821 atcatgatga agggaaaaac caccatgagc gcgcggttgc gtttatcgct gaaaagctga
     5881 atgaattcct tgcggaccag ctctctgatt ctcagccagt tcatagccct tcctttctca
     5941 gcagtctgta attcattgat gtcaggacaa taaacatgag cagcaacacc agcatgctcg
     6001 gccacaagta ggcaaatccg agattgcgca gataaatccc ggataggatg tcaatgaaat
     6061 atcgcgccgg cgtgatgaag gtgatggcct ggatgacggc cggcatattg acgatgggaa
     6121 aaacaaaatc ggaaagcaga aacgacggca agtacgttgc catgatcgca atctgattgg
     6181 ccagcaactg cgattgggtc acattggaaa tcaaaagccc cagcgacagc gcgacgccta
     6241 aatacagtga agacgataaa atcatcagcc agaagctgcc cttcatcacg atgccgaaca
     6301 gcagttggct caacagaatc gcaatcagca cgtcggtcaa cccgatgaag aaatagggca
     6361 gcgctttgcc cagcagcaat tctccggccc ggaccggcag agatttcaga gtctccatgg
     6421 tgccgttttc atattcacgc gcaatgacca gggacgtcag cagcg
//
LOCUS       DAOSME010000098         6317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_13514_length_6317_cov_17.674066, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000098 DAOSME010000000
VERSION     DAOSME010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13514_length_6317_cov_17.674066"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLG98_10495"
     CDS             <1..554
                     /locus_tag="PLG98_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOE33734.1"
                     /translation="FEVGDYGPSCVGKVFYVTDGGLHGLEAAPPNWYQGSEEASSVAW
                     INGDPQVDENDDIYQKTQSTTNGNTSEAIGTGLANSNAIVAQVEEAGGTDIPYAAALC
                     LDYSVGAGDYLFNDWFLPSRDELAQLFTNRDVKRSGGFLDEGYWSSSEYSQWDAWSQY
                     FSNGAQTGTYKSQGRLVRPIRKF"
     gene            815..1939
                     /locus_tag="PLG98_10500"
     CDS             815..1939
                     /locus_tag="PLG98_10500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE33735.1"
                     /translation="MSKILLKILTNMLLLISVFILPSFYPAGPAVYFRLFLLTVILIL
                     SVNLFKVLFIDRNIPKIIANTATVCMAVFVTFIVLEAAFMFIPRSHSADYTLASKLWY
                     AKYWKPINSMGFRDKEPAKSNHVILFVGDSFTAGHGLKSADERFSDIVAKKLNKKKND
                     FTVMNIAKPNLDSREEYDTMMHVLYLNRIKPEKIILQYCGNDIEGAAARHGLIFEGFK
                     PPSDMNRFLLLVGSGSYFFNYVYFLFPREYLGIPYLTYLDTAYKNNKVLSDHKKDLML
                     FIDYARKNSIQIIAVVFPFLADLAMSDAMYVNDITGFLESNGTNVINVSSLTANMPIP
                     ERIVNKNDGHASAKVNRMVAVEILKIMDGQNESHSSLSER"
     gene            complement(1960..3936)
                     /locus_tag="PLG98_10505"
     CDS             complement(1960..3936)
                     /locus_tag="PLG98_10505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE33736.1"
                     /translation="MIMRSSIDFVKKPLLAAALVAAIILVSGVLRILFYVKSPDVIFL
                     VNRAGAQWIKYDDEFELQAKPSGRIQCHFKYVFHTNETIREARISVQALKKAEVSFDG
                     VKIYSANDQFSRWKQVHDIKIPLDIAPGGHEIVISATSENSYAAILAYSKELQVRTGT
                     GWFASYDGKNWRAAVPVTTFTPPAVIMKFPTSFEALRAVWPYLSVVFIMVFCFSWTYG
                     SGKKVFPKLLQWIVQPCHVRWGLLGLWAILSINNMFKLNYQTGSDVWGHIDYIDYIAT
                     KGTLPLAPEGWQMFQAPLNYLLSAPLYAVLSKWLDLPSVVKIMTIIPVLCGLLQIEIV
                     YRTARLVFTQRKDLQIIAVIAGSLLPVHTYVCQYVGNEPLTACLVSVLILLSVELILP
                     DRKEHGNGYFVVMGVVWGLALLSKMTALLLAPVVLLVLIFHVWSLQGKLKNPLRPVLI
                     VFLVPVIVAGWYYLRNYLKLGNAFMGLFDPLQMMQWWQDPGYRIWPHLLSFGEALYRP
                     FYAGLTSFWDMFYSTLWLDGINSGLIDFIPWNENFMIAGSLLALLPSVFILTGLVNLA
                     LNKKTEYRNAVILSAASILIFLMAMIDMYIACPVYSRTKAGYTLGLLPCYAILIAAGA
                     EPFLRHRIIRSFALAFFACWAFAAYAAYFVVNAP"
     gene            4207..>4545
                     /locus_tag="PLG98_10510"
     CDS             4207..>4545
                     /locus_tag="PLG98_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33737.1"
                     /translation="MTKKTYIWLWSAFLACLILLPYLQMHSHDFIIWDDSFYIYDNPM
                     VTQGLNLLGVGWAFITFATANWHPLTWISHMLDSSIFGPTPFAAHLMNSLWYAGCILL
                     SFFLFLQLGAS"
     assembly_gap    4546..4645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4646..5483
                     /locus_tag="PLG98_10515"
     CDS             <4646..5483
                     /locus_tag="PLG98_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33738.1"
                     /translation="ISYLIYVRQTVWPSGLTVFYPYPLHIHLGDAFLACFFLIIVTIV
                     VVRQKQQYPYLLWGWLFYLGVLFPVIGIVQVGSQAHADRYMFLPQLGLILAVGLFLDK
                     TVVKMKLRRGLAVLMMIIAAVLMIITFRQVSYWKNSKSLFYRNLEVAGENELAHFNLG
                     SAYLENNELDMAVIHLTATAQMNPQDATTFNNLGIAYARKKIDALAEACFHRAILLDP
                     RTVQPYVNLAVLKTNQGRFEEALDHINTAVQLAPDYKEARDIRNALEKAVLRHKNNAD
                     GQ"
     gene            5473..6216
                     /locus_tag="PLG98_10520"
     CDS             5473..6216
                     /locus_tag="PLG98_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE33739.1"
                     /translation="MANNNKQEKVIPEISVVILCFQEGRRLSAFVNRTIEILESLPVS
                     WEIVLVANWDNENDDTPGEAKKIAAQKNHIKVVSEFKKGGMGWDARKGFEQASGRFIC
                     LIDGDGQMPPESIIGVYHKIIYDHCDLVMTYRTQRGDSLLRKINSYTYNFAFHILFPA
                     VNVRDANSKPKIFTRNTYSKMQLNADDWFLDAEILIQCTRLKLKIAEIPVTFDKCLSR
                     KSFVKIDAIWEFIKNLLSARIKEYFIKNN"
BASE COUNT         1786 a         1480 c         1398 g         1553 t
ORIGIN      
        1 cgtttgaggt aggtgattac ggacccagct gtgtcggcaa ggtattttat gtaaccgacg
       61 gaggactgca cggtctggag gctgcaccgc cgaactggta tcagggcagt gaagaggcat
      121 cttctgtggc atggattaat ggggatcccc aggttgatga aaatgacgat atttaccaga
      181 aaacacaatc cacaaccaac ggaaacacct cagaggcaat cggaacaggc ttggccaaca
      241 gtaatgccat tgtagcgcaa gtggaagagg ccggcggcac cgatattcct tatgcggcag
      301 cattatgtct ggattacagc gttggcgcag gagactatct gtttaacgac tggtttctgc
      361 cttccaggga cgaactggcc cagttattca ccaaccgcga cgtgaagagg tccggaggat
      421 ttctcgatga agggtactgg agttcctcgg aatacagcca gtgggatgcg tggtctcagt
      481 atttcagcaa cggggcacaa accggaacgt acaagagcca gggacggttg gtccggccga
      541 ttcgcaagtt ttaattttac gttttttgcc aatgacttaa aaaacgataa aggcgacatc
      601 gcctttatcg tttttttgta agatctgccc ggctttgatg gtgtttgtcc tgaagcgccg
      661 ccgtgatgca gaaaaaatca ttcacgcatc ttaaaggcat gccggcgcaa actgtccctg
      721 caaacagaac cggggacaga tcctatattc atatggcaat gataaaaata tttttcttat
      781 attccggaca aaattaattt ataaatacaa agccatgtca aaaatattat taaaaattct
      841 gaccaacatg cttttgctga tatccgtctt tattttaccg tcattttatc cggccggccc
      901 ggctgtttat ttcagattat tcctgcttac agtaatacta atattaagcg tcaatttgtt
      961 taaagtcctt ttcattgacc gcaacatccc taaaatcatc gccaacacgg caaccgtctg
     1021 catggcggtt tttgtaacgt ttattgtgct ggaagcagca ttcatgttta ttccccgttc
     1081 ccattccgcc gattacactt tagcatccaa actgtggtac gcaaaatact ggaaaccgat
     1141 caattccatg ggattcagag acaaagaacc ggcaaaaagc aaccatgtta ttttatttgt
     1201 cggtgattca tttaccgcag gccatggctt aaaatccgct gacgaacggt tttccgacat
     1261 cgtggcaaag aaactcaata aaaagaaaaa tgattttacc gtcatgaaca tcgcgaaacc
     1321 caatctggat tcccgggaag aatatgacac catgatgcat gtcctttacc tcaaccggat
     1381 caagcccgag aaaataatat tgcaatattg cggaaatgat attgagggag cggccgccag
     1441 acacgggctg atatttgaag gctttaaacc gccgtccgat atgaaccggt ttctcctgct
     1501 cgtcggatcc ggttcttact ttttcaacta cgtttatttc ctttttccca gagaatacct
     1561 gggcattccc tatttgacgt atctcgacac ggcatacaaa aataataagg ttttgtccga
     1621 ccataaaaaa gatctgatgc tgttcattga ttacgccaga aaaaactcca tacaaattat
     1681 cgccgtcgtt tttccgtttc tggctgacct tgcaatgagc gacgcgatgt acgttaacga
     1741 tattaccggt tttctggaaa gcaacggtac aaacgtcatt aatgtttctt ccttaaccgc
     1801 gaacatgccg atcccggaaa gaatcgtcaa taaaaatgac ggacacgcct ccgcaaaagt
     1861 caacagaatg gttgccgtcg agattttgaa aatcatggac gggcagaatg aatcgcatag
     1921 tagtctttct gagcgctaaa aaaacattcc agcagattat tatggggcgt tgacgacaaa
     1981 ataggccgcg tacgcggcga aagcccagca cgcaaagaaa gccagcgcaa aggaacgaat
     2041 gattcggtgg cgcagaaaag gttccgcgcc cgccgcaatg aggattgcat agcagggcag
     2101 cagccccaac gtatagccgg cttttgttct gctgtacacc ggacaggcaa tatacatgtc
     2161 aatcatggcc atcagaaaaa tcaggatcga agcagcggac aatatgacgg cgtttcggta
     2221 ttccgtcttc ttattcaggg caaggtttac cagacccgtc aggataaaga cactgggcag
     2281 taaagccaac agagatccgg caatcataaa attctcattc cacgggatga aatcgatcaa
     2341 tcccgaatta atgccgtcaa gccataaggt ggaataaaac atgtcccaga agctcgtcag
     2401 gccggcataa aagggtctgt acaaggcttc gccgaaggac agcaaatgcg gccatatccg
     2461 ataaccggga tcctgccacc attgcatcat ttgcagcgga tcaaatagcc ccataaaggc
     2521 attgccgagt ttcaggtaat ttctcaaata gtaccagccg gcaacgatca ccggaacaag
     2581 aaaaacaatc agaaccgggc gcaacgggtt ttttaatttg ccttgaagcg accatacatg
     2641 aaaaatcaac accagcagca ctaccggcgc cagcagcaaa gcagtcatct tggaaagaag
     2701 ggccaatccc catacaaccc ccatcacgac aaaataaccg tttccgtgtt ccttccgatc
     2761 gggcagaatc agttctacgc tcaacaaaat cagcacagac accagacaag ccgtcagagg
     2821 ttcattgccc acatactgac agacgtaggt gtgaaccggc agcagggaac cggcgatgac
     2881 ggcaatgatc tgcaaatctt ttctttgtgt aaaaaccaat cgcgccgtgc ggtaaacaat
     2941 ttctatctgg agaagcccgc agaggaccgg aatgatcgtc atgattttaa ccacagacgg
     3001 taaatccagc catttgctga gcacggcgta aagcggcgca ctcaacagat aattgagcgg
     3061 ggcttgaaac atctgccagc cttccggagc cagaggaagt gtgcctttcg tcgcgatata
     3121 atcgatataa tcaatatggc cccagacgtc gctgcccgtc tgatagttga gtttgaacat
     3181 gttgttgatc gataatatcg cccacaagcc caacagaccc cagcgcacgt gacaaggttg
     3241 aacgatccat tgcagaagtt ttggaaatac tttcttacca ctgccgtagg tccaactaaa
     3301 acaaaacacc atgatgaaaa caacggacag atagggccat acggcgcgaa gcgcctcaaa
     3361 cgatgtcgga aatttcataa taaccgcggg cggtgtgaac gttgtcaccg gcacagccgc
     3421 acgccagttc ttaccgtcgt aagacgcaaa ccatcccgtt ccggtccgga cctggagttc
     3481 tttactgtac gccaaaatgg cggcataaga attttcactg gttgcggaaa taacgatttc
     3541 atggcctccc ggtgcaatgt ccaagggtat tttgatatca tgaacctgct tccatcgact
     3601 gaattgatca ttcgcggaat aaatttttac gccgtcaaag gagacctctg cttttttcag
     3661 cgcctgaaca gagatccggg cctcccggat tgtttcattc gtatgaaaaa catatttgaa
     3721 atgacattgt atccggcccg acggtttggc ctgcagttcg aattcatcgt cgtattttat
     3781 ccactgagct ccggcacgat tgacaagaaa aatgacatcg ggagatttta cataaaacag
     3841 gattcgtaag acaccggaaa caagaatgat cgcggcaacc agagccgcgg ccagtaaagg
     3901 ttttttaacg aaatcgatgg atgaacgcat gatcatttgt aaaaaaaccg tctttccgaa
     3961 aaatctaagt tatcctgtat attgtataca agtcaaaata acgggatttt gatcgttgga
     4021 aaagttatat gggatatccc gttgacgtgt caatcagaaa ctgatgatgc gggttcacga
     4081 ccgtttgaac atcaaacgga ttgtgccgat tgttttatgt tgacaccccg caacagattc
     4141 atataaattc gcatcataca gatcaattga cataaagtgt ggccaggcac atcattggaa
     4201 aacggaatga cgaaaaaaac atacatatgg ctatggtcgg cgtttctggc atgtttgata
     4261 ttgttaccct acctgcagat gcattcgcat gattttatca tttgggatga ttcattttac
     4321 atttacgaca atccgatggt cacgcaaggc ctaaatttgc tcggcgtcgg ttgggctttc
     4381 atcacatttg caacggcaaa ctggcatccg ttgacctgga tcagccatat gctggattcc
     4441 agtattttcg gcccaacgcc gtttgccgct catctgatga acagcctctg gtatgcaggc
     4501 tgtattctct tgtccttctt tttatttttg cagctcggcg cctctnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnngatct cttatctgat ttatgttcgc cagacggtct
     4681 ggccttccgg actgaccgta ttttatccct atcctcttca cattcatctc ggcgatgcct
     4741 ttctggcctg cttctttctg atcatcgtaa ccatcgtcgt cgtccgtcag aagcaacaat
     4801 acccctatct tttatggggc tggttattct atctgggcgt tttgtttccc gtaatcggca
     4861 ttgttcaggt gggcagtcag gcccacgccg accggtatat gtttttaccc caactgggac
     4921 tgattctggc ggtcggactt tttctggaca aaaccgttgt aaaaatgaaa ctgcgccgcg
     4981 gattggccgt tctgatgatg atcattgccg cggttctcat gattattaca ttcaggcagg
     5041 tgtcctactg gaagaacagc aaatctttat tctaccggaa tctggaggtt gccggcgaaa
     5101 atgaactggc ccatttcaat ctgggctcgg cctatctgga aaacaacgaa ttggacatgg
     5161 ccgtcatcca tttaacggcc acagcgcaaa tgaaccctca agatgccaca acgttcaaca
     5221 acctgggcat cgcttacgca cggaagaaaa tagacgcgct tgccgaagca tgttttcacc
     5281 gggccatttt actcgatccc cggacggtgc aaccgtatgt caatcttgcc gttctgaaaa
     5341 caaatcaggg acgctttgaa gaagcgcttg accacatcaa cacagccgta caactggcgc
     5401 cggattataa agaggcaagg gacatacgaa acgcattaga aaaggcggta ctccgtcata
     5461 aaaacaacgc cgatggccaa taacaataaa caagaaaagg taattccgga gatatcggtc
     5521 gtcatcctgt gctttcagga aggtcgaaga ttaagtgcgt ttgtcaatcg aacgatcgag
     5581 atactggaga gcctgccggt atcctgggaa attgtcctgg tcgccaactg ggacaacgaa
     5641 aacgacgata cccccggaga agccaaaaaa atagctgcgc aaaaaaatca tatcaaagtt
     5701 gtcagcgaat tcaagaaagg cggcatgggc tgggatgccc ggaaaggctt tgagcaggcc
     5761 tccggaagat ttatctgtct tatcgacgga gatggtcaaa tgccgccgga aagcatcatc
     5821 ggagtctatc acaaaataat atatgatcat tgcgatctgg tcatgaccta ccggacgcag
     5881 cggggagaca gcttgttgag aaaaatcaat tcgtatacct ataatttcgc cttccacatt
     5941 ctatttccgg ccgtcaacgt cagggatgcc aactccaaac cgaagatttt cacccggaac
     6001 acctactcga aaatgcagct taatgccgac gactggtttc tggatgcgga aatattaatt
     6061 caatgcacaa gattgaaact caaaatcgca gagattcccg tgacctttga taaatgcctc
     6121 agccggaaat ctttcgtgaa gatcgatgcc atttgggaat ttatcaagaa ccttttatcc
     6181 gcaagaataa aggaatattt tatcaagaat aactaaaaaa atagacattg ctcttttcag
     6241 cgcaacattt ccttgacacg aaattttata ttacttatat cttaaaaact taaacgtatt
     6301 gctttaatcc ataatcc
//
LOCUS       DAOSME010000099         6206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_13822_length_6206_cov_15.445293, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000099 DAOSME010000000
VERSION     DAOSME010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13822_length_6206_cov_15.445293"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..940)
                     /locus_tag="PLG98_10525"
     CDS             complement(128..940)
                     /locus_tag="PLG98_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE33740.1"
                     /translation="MLKYFYIESTKISCWTNTENVDAQKNNLVFIHGSGGNYSIWSHQ
                     YGKLHKRYNIAAVNLPGHGPSEGDGEQTIQNYCDWMRKILAALGLTKPVLVGHSLGAA
                     IALDFALRYPSDIAGIVCIGAGLKMPVNAFFLEYLQTHPPDIPAEIIDLICKFSLAKE
                     NRERLSGPLRKSLALSRVDVLYGDLLACNTLDLSHDAARISVPVFIVCGAEDKMTPPD
                     LSRALARQIGGAKLSMIEGAGHTVMIEKPVEFNNALEKFAESVFPASTPLKN"
     gene            1311..2129
                     /gene="proC"
                     /locus_tag="PLG98_10530"
     CDS             1311..2129
                     /gene="proC"
                     /locus_tag="PLG98_10530"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOE33741.1"
                     /translation="MLKDKKAVIIGGGKMGGIIARSLVSRQIISAKNIHVTDIDASRL
                     KVLRSSLKIKTSSDNPRAAGESDIVILAVKPQNMAATLADIKSSMDKSKVVISIAAGI
                     TTKFIETHLGKGIRVVRVMPNTPALIGEGAAAVAAGRHAKAGDLRIARTIFNAVGISV
                     EVQEKLMDAVTGLSGSGPAYFFLIIEALIDAGVRNGLSRDLAKKLATRTMLGAARLCI
                     ESDKEPAELRAMVTSPNGTTFAGLNVMESKNIHAILAATVTAATRRSRELSKGT"
     gene            complement(2144..3925)
                     /locus_tag="PLG98_10535"
     CDS             complement(2144..3925)
                     /locus_tag="PLG98_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOE33742.1"
                     /translation="MLVNAVHKEQRRMATVNEKGILVDFNIEMPTKEPITGNIYKGKV
                     LKVERGLQAAFVDYGGSKDGFLPLKDVSHDYFTEPEKGQSNGKPALKVGQEIIVQAVR
                     EAIGNKGALLTSYISLPGRYLVLLPNKPGSGISRKVEDEEDRQKIKNMMKQIKIPETM
                     GFIVRTAGYNRTKQEISRDYQHLVRLWKEINKKAKSIGKTSMIYQETDFGVRSIRDYL
                     TSEIEEIWVDDLETFRKMRAYLRAISPRYIKLLKHYKEKTPLFDRHNLEDQIRVIYQE
                     RVELKSGGHIVINPTEAMITIDVNSGRGSNKKNIEETAFKTNIEACEEIARQLRLRDL
                     GGLIAIDFIDMMDKKHIAEVEKSFKKALSIDKARIQLSRISKFGILELSRQKKQSTIQ
                     EISYMKCPYCKGSGLRPSLEYAALGAFRKVESEAVKGLCCELKINLPHEIADYLLNRK
                     RSELLDLESSFDVTLTICGNSEIPWDRVDIQQIAKEHIDSADHPQEADEPKGQEAPAT
                     AEEEKAVPGGEKPARKRGRRRPRHKKRKPAESAPAGVLPDNVSDGAAEDGLSNLLIIQ
                     PAENQKDAASEKKASPDDPAAAPEDHK"
     gene            complement(4033..4641)
                     /locus_tag="PLG98_10540"
     CDS             complement(4033..4641)
                     /locus_tag="PLG98_10540"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOE33743.1"
                     /translation="MLFSINSQNPQMRLIKKAADILRDGGIVIYPTDTVYGLGCDLSN
                     KKGIEKIYELKRRNRKQPLSFVCSDLKHISEYAKVTDYAYKTMKRLLPGAYTFILEAS
                     RLVPKIILPKRSTTGIRVPDNQICLALVRELGQPIISTSVKTEKGETLGDPAVIKEYF
                     GKTVDLIIDGGIIVPEPSSVISLVDDNIDIIRKGKGDISAFL"
     gene            complement(4807..>6206)
                     /gene="cimA"
                     /locus_tag="PLG98_10545"
     CDS             complement(4807..>6206)
                     /gene="cimA"
                     /locus_tag="PLG98_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOE33744.1"
                     /translation="NAAFKNSRLTAFGSTRRAHTKTESCPNLKALIKAGTPACAIVGK
                     SWDLHVTDILKTDLNENLAMIADSIAYLKSMDKEVLFDAEHFFDGYKNNSRYATRVVK
                     TALAAGADFIVLCDTNGGCLPHEICALVKRISAHVPFEKMAIHVHNDGGLAVANTLAA
                     VQKGVAMVQGTVNGYGERCGNADLIAVIANLQLKMGKKCLPPESIKQLTNLSHYISDV
                     ANVPPLNSRPFVGRSAFAHKGGVHVSAVVKNSAAYEHIEPELVGNHRRVLMSDLAGKS
                     NVEYKAKELGIDLEKNDAVAKKIVHEVKLMEDRGFQFDAADGSLSLLIKKTIGEFVAP
                     FNLETFSVITSRKENNPCLSQATIKISVAGEEELTAAEGNGPVNALDHALRKALTKFY
                     PQIKNMRLVDFKVRTLEGSEGTGAGVRVLVDSTDGEDVWSTIGVSENIIEASWQALVD
                     SIQYKLSKENNLNRR"
BASE COUNT         1358 a         1646 c         1606 g         1596 t
ORIGIN      
        1 ggattgaccc aggagatcag ggcacggaag ataatgatcc acatgtaaac cgtcaaaaca
       61 atatcggcga ctttggccac agcgatgata aaattgctca aaacaaacat aacgcgtctc
      121 ctgtcattta attttttaac ggcgtcgaag ccgggaaaac ggattcggca aatttttcca
      181 gagcattgtt gaattccaca ggtttttcaa tcatcaccgt atgaccggcg ccttcaatca
      241 tggacagttt cgccccgccg atctgtcggg caagcgcccg cgataaatcc ggcggcgtca
      301 ttttgtcttc cgcaccgcaa acgatgaata cgggaacgct gatcctggct gcgtcgtgcg
      361 ataagtccag cgtgttgcag gccagcaggt cgccgtataa aacatccacc cgggaaagcg
      421 caaggctttt gcgcagaggc ccggaaagcc tttcgcgatt ttctttggcc agtgaaaatt
      481 tgcagatcag gtcgatgatt tcagccggta tatccggcgg atgggtctgc aggtactcca
      541 ggaagaaagc attcaccggc atcttcaatc ccgcgccgat acagacaatg ccggcaatat
      601 ccgaaggata gcgcagggca aaatccagcg cgatggccgc gcccagcgaa tgtcccacca
      661 gcaccggttt tgtcagaccg agcgccgcaa ggatttttct catccagtcg cagtagtttt
      721 gaatcgtctg ctcgccgtca ccttcggaag gaccatgccc cggaagattg acagcggcga
      781 tgttatatcg tttgtgcagt ttgccgtact gatgagacca gatgctgtaa ttgccgccgg
      841 agccatgaat aaaaaccaga ttattttttt gcgcgtcaac attctccgta ttggtccagc
      901 aggaaatctt cgttgattcg atataaaaat atttgagcat gccgtcaccc tttcacccgt
      961 taactatcac aaaaacaaaa tcgaatgcaa cggaagaatc atggatttgc cgccctccgc
     1021 tgcgcgggat aatttaatga gccttgattt ttaccctgaa gggcactgcg ccgaaacgaa
     1081 gtgcccggaa aatcttagcg cgaactatcc cgctcagctc agcttcgggg atagttctct
     1141 ttgggatccc attcgggatc cacaggttca ctatcccgcc accttcaggc ggtagggaca
     1201 aacttcggca cactccattt ctgaagtttg gctatcatga taaaccgctt gacctgaccg
     1261 gcgtaagcag gataatgaac ggcataccaa ggcatggagg cattgagcgt atgttgaagg
     1321 ataaaaaagc ggtgatcatc ggcggcggta aaatgggcgg tatcatcgcc cggagcctgg
     1381 tgagccgtca aatcatttcc gccaaaaaca ttcacgttac cgatatcgat gcctcgagac
     1441 tcaaggttct gcggtcgtcc ctgaagatca agacctcgtc cgacaatccg cgggcggcgg
     1501 gcgaatccga cattgttatt cttgccgtca aaccgcaaaa tatggccgcg acgctggcgg
     1561 acattaaatc gtctatggat aagtcgaagg tggtgatttc gattgccgcc ggtatcacga
     1621 caaagtttat cgagacgcat ctgggcaaag gaattcgtgt ggtcagagtg atgcccaata
     1681 cgccggcgct tatcggtgaa ggagccgcgg cagtggccgc cggccgccat gccaaagcgg
     1741 gtgacctccg gatcgcgcgc acgatcttca atgccgtggg catcagcgtg gaagtgcagg
     1801 aaaaactcat ggacgcggtg accggcttga gcggcagcgg ccccgcctat ttttttctga
     1861 tcatcgaggc gttgattgac gccggggtcc gaaacggatt atcccgggat ctggcgaaaa
     1921 aactggcgac gcgaaccatg ctgggagctg cgcgtctgtg catcgaaagc gataaggaac
     1981 cggcggagct cagggccatg gtgacatcgc ccaacggcac gacattcgcc ggattgaacg
     2041 tgatggaatc gaaaaatatt cacgcgattc ttgccgcgac cgtgacggca gccacccggc
     2101 gctccagaga actgtctaaa ggaacgtaat cttcgcaagt ttgttattta tggtcctccg
     2161 gcgcggccgc agggtcgtcc ggagaggctt tcttttcaga agcggcgtct ttttggtttt
     2221 ccgccggctg tatgatcagc aggtttgata acccgtcctc ggcggcgcca tcggagacgt
     2281 tatccggcaa aacaccggca ggtgctgatt cagcgggctt ccttttttta tgacgcggtc
     2341 tgcgccgtcc tctttttctg gcgggttttt ccccgccggg cacagccttt tcctcctcgg
     2401 cggttgccgg agcctcctgt cccttgggct catccgcctc ttgcggatgg tcggcgctgt
     2461 caatgtgctc cttcgcgatc tgctgaatgt ccacccgatc ccagggaatt tcagaattcc
     2521 cgcaaatcgt cagcgtgaca tcaaatgacg attccagatc aagcagttcg cttctcttgc
     2581 gattgagcag gtaatcggcg atctcatggg gaaggtttat tttcaattcg cagcagagcc
     2641 ctttgacagc ttccgattcg actttccgaa aggcccccag cgccgcgtat tccagagaag
     2701 gacgcaagcc gctccctttg caatacgggc acttcatgta gctgatttcc tgaatggtcg
     2761 attgtttttt ctgccgggat aattcgagga ttccgaactt ggaaatccgg gaaagctgaa
     2821 tgcgggcctt atcgatgctc agcgccttct tgaaggattt ttccacctcg gcgatatgtt
     2881 ttttatccat catatcgata aaatcgatgg cgatcagccc gccgaggtcg cgtagcctga
     2941 gctgccgggc aatttcttca caggcttcaa tattcgtttt aaaagcggtt tcttcgatat
     3001 ttttcttgtt ggagccgcgg ccggaattga catcaatcgt gatcatggcc tccgtcgggt
     3061 tgatgacgat gtgtccgccg gatttcagtt ccacgcgttc ctgatagatg acgcggattt
     3121 gatcttccag attgtggcgg tcgaaaagcg gcgtcttctc cttgtaatgc ttgagcagtt
     3181 tgatgtaacg cggcgagatg gccctcaggt aggcgcgcat cttacggaat gtctccagat
     3241 catccaccca gatttcttcg atttccgatg tgaggtaatc gcggatactg cgcacgccga
     3301 aatccgtttc ctgataaatc atggaggtct tgccgatgct tttcgccttt ttgttgattt
     3361 ccttccagag acgaaccaga tgctgatagt cgcgggagat ctcctgcttg gtccggttgt
     3421 agcccgccgt ccggacgata aatcccatcg tttcggggat ttttatctgc ttcatcatat
     3481 ttttgatttt ctggcggtct tcctcatctt ccactttgcg ggaaatgccg ctgccgggtt
     3541 tgttgggcag caaaaccaga tatcgtccgg gcagggaaat gtaggacgtg agcagcgccc
     3601 ccttgttgcc gatggcttcc cggacggcct gcacgatgat ttcctgtccg acttttaaag
     3661 ccggtttgcc gttggattgt cctttttccg gttcggtgaa atagtcgtga ctgacgtcct
     3721 tgaggggaag aaaaccgtct ttgctgccgc cgtaatccac aaaggcggcc tgcaggccgc
     3781 gttcaacttt cagaaccttg cctttgtaaa tgttgccggt gatcggttcc ttggtcggca
     3841 tttcaatatt gaaatcgacc agaattccct tttcgttaac cgtagccatc cgtctctgtt
     3901 ctttatgaac agcgttaacg agcataatat gtttcataaa tgtccttttt gcggcggctt
     3961 ttttgcgcgc acccgcgctc cggcagcgca tttaaacatc tgcaggttaa aattccgttc
     4021 cgcgttttat atttagagaa aagcgcttat atcgcctttt ccttttctta tgatgtcgat
     4081 attgtcatcc accaggctga tgacgcttga cggttccggc acaatgatac cgccgtcgat
     4141 aatcagatcg actgttttac cgaaatattc cttaatgaca gcagggtcgc ccagagtttc
     4201 tcccttttcc gttttcacgc tggtgctgat aatgggctgt ccgagttccc ggaccagcgc
     4261 cagacaaatc tgattgtcgg gtacccttat acccgttgtt gaccgtttag gcaaaataat
     4321 ttttgggacc agcctggagg cctccaaaat aaaggtgtac gctcccggca gaagcctttt
     4381 catggtttta taggcgtaat cggttacttt ggcgtactcg ctgatatgtt taagatcgga
     4441 acagacaaag cttaacggtt gctttcggtt tctccgtttc agttcgtaaa ttttttcaat
     4501 cccttttttg ttggacaaat cgcagcccag accgtaaacc gtgtcggtag gataaatgac
     4561 gatgccgccg tcgcgcagaa tatcggcggc cttcttgatc aggcgcattt gcggattctg
     4621 gctgttgatg gaaaaaagca ttctctgtcc gtcccttttg aaccttgaag aaactcatat
     4681 accaaaaaag gttcttgcgc aacggaagct tcacgggacg tccgccatgc aggatttgtg
     4741 atcatccccg gttgtctgtt ccgcgcggca ccgcgcccga cacgaaagcc caaagaaggt
     4801 tgagcatcat cttctgttga gattgttctc cttgctcagc ttgtattgaa tgctgtccac
     4861 cagcgcctgc cagctggcct cgatgatatt ttcggagacg ccgatggtgc tccagacgtc
     4921 ttcgccgtcg gttgaatcca cgagcacccg gacacccgcg ccggtgcctt ccgacccttc
     4981 cagcgtgcgc accttgaaat ccaccaggcg catatttttg atttgcggat aaaatttcgt
     5041 cagcgctttg cgcagggcgt gatccagcgc attgaccggg ccgtttcctt ccgcggccgt
     5101 caactcctct tcgccggcca ccgagatctt gatggtcgcc tgagacagac agggattgtt
     5161 ttccttacgc gacgtaatca cgctgaaggt ttccagattg aacggcgcga caaattcgcc
     5221 gatggttttc ttgatcagca gagaaagcga accgtccgcg gcgtcaaact gaaatccgcg
     5281 gtcttccatg agcttgactt catgcacgat ctttttggca acggcgtcgt ttttctccag
     5341 atcaatgccc agttctttcg ctttatattc cacgttgctt tttcccgcca ggtcggacat
     5401 cagcacgcgc cggtgattgc cgacgagttc cggctcaatg tgttcgtagg cggcagaatt
     5461 tttcaccacg gcgctgacat gaacgccgcc tttgtgcgcg aacgcgctgc gcccgacaaa
     5521 cggccgggaa ttcagcggcg gcacattggc cacatcgctg atataatgag acagattcgt
     5581 cagttgtttg atggattccg gcggcagaca ttttttcccc atctttaact gcagattggc
     5641 aatcaccgca atcaaatcag cgttaccgca tcgttcaccg taaccgttga ccgttccctg
     5701 aaccatcgcg acacctttct gaacggcggc aagggtattc gcaacggcaa gaccgccgtc
     5761 attgtgtaca tgaatggcca ttttctcaaa aggaacatgc gcggaaattc tcttcaccag
     5821 ggcacaaatt tcatgaggga gacatccgcc gttggtatcg caaaggacga taaaatcggc
     5881 tccggcggcc agcgccgttt tcaccacccg ggtggcatac cgggaattgt tcttatagcc
     5941 atcgaaaaaa tgttcggcat caaagagaac ttctttgtcc atggatttca gataggcgat
     6001 ggaatcggca atcattgcca gattttcgtt gaggtctgtc ttgagaatat cggtgacgtg
     6061 caagtcccac gatttcccga caatggcgca tgcgggcgtt ccggccttga tcaaagcctt
     6121 taaattcggg cacgattcgg ttttggtatg cgcgcggcgc gtgctgccga aggcggtcag
     6181 ccgcgaattc ttaaatgcgg cgtttt
//
LOCUS       DAOSME010000100         6133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_14054_length_6133_cov_18.399967, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000100 DAOSME010000000
VERSION     DAOSME010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14054_length_6133_cov_18.399967"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..413
                     /locus_tag="PLG98_10550"
     tRNA            327..413
                     /locus_tag="PLG98_10550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:361..363,aa:Leu,seq:tag)
     gene            463..1788
                     /gene="tig"
                     /locus_tag="PLG98_10555"
     CDS             463..1788
                     /gene="tig"
                     /locus_tag="PLG98_10555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOE33745.1"
                     /translation="MSQVVINVEDLSPVKKKVSLEIPWDDVKNELDAVYQEVGKKAKI
                     KGFRPGKIPRKILETYFKDQAEEETITNIVNKYYWQALEGKGIVPVSRPEINQDGIKD
                     NTNFSFSASFETEQDFEPRHYKGIDVEKEQIKVTDDDVQKRIDEIRQMFATMEEVTDD
                     RPADKGDFVVMDFEGTLNGEKYKELKADDYFLEIGSQKFVPGFEDQLIGMKKGEARDI
                     HVSFPTDYHESRFAGQEVVFHVTVKSIREKKLPENDDSLIKNFEKYESFEDFKNDVRK
                     TLEDKAREMDKVKLQNKITDILVSENELEVPSALVERQIYYMMMETQQKMLSAGIDEN
                     TAMDFNLKMKDKYKPDAEKIVKSFLVLKKIAEKESITATDGDLESYLQELAQHSGRDY
                     ESFQKMYNIEERKDSLMMELVQKKVFDFIEQNANIKVVEKVGLNTEAKS"
     gene            1800..2399
                     /locus_tag="PLG98_10560"
     CDS             1800..2399
                     /locus_tag="PLG98_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOE33746.1"
                     /translation="MVVEQDGRGERAYDIYSRLLKDRIVFLGTAIDDNVANLIVAQML
                     YLEAENPDKEIFFYLNSPGGIVSAGMAIYDTMRYIKSPISTVCMGQAASMAALLLAAG
                     ERKKRFALPHSRILIHQPLGGFQGQATDIDIQAKEILRLKDELNKILADLTQQPLAKI
                     VNDTERDYFMSSEQAKEYGLIDEIIIRKPDASAVRAKEK"
     gene            2400..3662
                     /gene="clpX"
                     /locus_tag="PLG98_10565"
     CDS             2400..3662
                     /gene="clpX"
                     /locus_tag="PLG98_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOE33747.1"
                     /translation="MIKKGKNNRMDDGMLFCSFCGKMQSEVRKLVAGPTAYICDECIE
                     LCRYIVEEEDDKLIEKDAQKALPYPKEIKKFLDQYVIGQEKTKKILSVAVYNHYKRLF
                     SRVESDGIEIQKSNVLLIGPTGSGKTLLAKTLAKFLNVPFTIADATTLTEAGYVGEDV
                     ENIILSLLQNADYDVARASKGIVYIDEIDKIAKKSGNPSITRDVSGEGVQQALLKIME
                     GTMANIPPKGGRKHPQQEFIQVDTTNILFICGGAFNDLESIISKRLGVNAMGFGAEIK
                     SKKEKRIGELLSEVRSEDLLKFGLIPEFIGRLPVIATLDELDEAALIKILVEPKDAII
                     KQYKKLFALENVVLKFTDGALKAVASLSTERKSGARGLRSILENTMLEVMYDIPSQNN
                     IKECVINEDVVLKKEKPILIYESKSESA"
     gene            3681..6086
                     /gene="lon"
                     /locus_tag="PLG98_10570"
     CDS             3681..6086
                     /gene="lon"
                     /locus_tag="PLG98_10570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOE33748.1"
                     /translation="MFDEKIENEQEKTAIIPLLPLRDVVMFPHMIVPLFVGREKSIAA
                     LESAMKYEKSIFMVAQKNAKDDDPKKEDIYHVGTIGIIIQLLRLPDGTVKVLVEGKTR
                     GMIKEYLPNSDFFLVKVNEVDDIDDGNDVKKQALMRSIKESFELYLKLSKKIHVEMLG
                     TVVAIEDASKLADVVITHLNVKLEDKQKIIEIFDISERLEAIYSLMLSEIEILQVEEK
                     IKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFRNEINELEKRLKQKKMSEEAVKK
                     VKQELKKLQMMTPMSAEATVVRNYIDWILDMPWSEKTENKYTLKESEEILDADHFGLK
                     NVKERIIEYLAVQSLVKKNKGSILCLVGPPGVGKTSIAKSVARATNRKFVRISLGGVH
                     DEAEIRGHRRTYIGAMPGKIIQLLKKAGSNNPVFCLDEVDKLSSDFRGDPSAALLEVL
                     DPEQNFAFNDNYLDLDYDLSEIMFITTANVLQTIPAPLQDRMEVIRIAGYTEPEKLQI
                     AKKYLIAKQVEANGLVMENIEFTHGALLKIIREYTREAGVRNLDREIASICRKVAKEI
                     VNNDKKGRVVIGSKSVGKYLGVPKFHHGEIEEKNKIGLTVGLAWTEVGGELLSIEASI
                     MEGNGKMIMTGKLGDVMQESVQAALSYIRSRADEFGLTKNFYRKIDIHVHVPEGAIPK
                     DGPSAGVAMTTSIASVLLRKKVRADLAMTGEITLRGRILPVGGIKEKVLAAHRGNIKT
                     VIIPKDNQRDLAEVPQNVQKALKIIFAEHIDDVLKLAFVQDEGEMRPAVDADVPMRSQ
                     SVN"
BASE COUNT         1902 a         1254 c         1565 g         1412 t
ORIGIN      
        1 gtatctgaat ggggttcaga gggccggtgg ttcaaatcca ctcgccccga ccaattaatt
       61 taaggggcaa accacctatc cggtttgccc tttgtgttta aataccggtt gaatgaaatg
      121 ggcattcgac aaggcatcct ttcgcgattc atctggtttc tgtacgatga gcgtccggaa
      181 aagaaaattt attttttctt gcataaaagc agacaatcaa ataaataatg gggccgtaaa
      241 atcttaaata gatgaaattt cttgtgattt tgcaaaataa tctgcgttga caatcttaaa
      301 ctaagtgaat atataagaat gtccatgcga gagtggcgga actggcagac gcactggact
      361 taggatccag cccgcgatgc ggaatagggg ttcaactccc ctctctcgca ccagatttta
      421 aaaaacgaaa aagcataatt caacgataaa ggagaaggga tagtgagtca ggttgttata
      481 aatgttgagg atttaagccc ggtcaaaaag aaggtgtcac tggaaatacc ctgggatgac
      541 gttaaaaatg aactggatgc cgtttatcag gaagtgggta aaaaggctaa aattaaaggc
      601 tttcgccccg gcaaaattcc ccgtaaaatt ctggaaacat attttaaaga tcaggcggaa
      661 gaagagacca tcaccaatat tgtcaataaa tactactggc aggctttgga aggaaagggg
      721 attgtgcccg tttccagacc ggaaatcaat caggacggta tcaaggataa tactaacttt
      781 tctttttcag cgtctttcga aacggaacag gattttgagc cccgtcatta caaaggcata
      841 gacgtggaga aagaacagat taaagtgacc gatgatgacg tgcaaaaaag aatcgacgaa
      901 ataaggcaga tgtttgccac catggaggaa gtgaccgatg accgcccggc ggataagggt
      961 gatttcgttg tgatggattt cgaaggaact ttgaacggcg aaaaatacaa ggaactgaaa
     1021 gcagacgatt attttctgga aatcggatcc cagaaatttg tccccggttt cgaagatcag
     1081 ctgatcggaa tgaaaaaagg tgaagcaaga gacattcacg tatcgtttcc gacggactat
     1141 catgaaagca gatttgccgg ccaggaagtg gtatttcacg ttaccgttaa gagcatcaga
     1201 gaaaaaaaat tgcccgaaaa cgacgacagt ttgattaaaa attttgaaaa atatgaatct
     1261 tttgaagatt tcaagaatga tgtccggaaa accctggaag acaaagcccg cgagatggac
     1321 aaggttaagt tgcaaaacaa aataacagac attctggtca gcgaaaatga attggaagtt
     1381 ccctccgcgc tggttgaacg gcagatctat tacatgatga tggaaacgca gcaaaaaatg
     1441 ctctctgccg gtatcgatga gaataccgcc atggacttca acctgaaaat gaaggacaaa
     1501 tacaaacccg acgcggaaaa aatcgttaaa tctttcctgg tgttaaagaa gattgcggaa
     1561 aaagaatcca ttaccgcgac ggacggcgat ctggaaagct atcttcagga actggcgcaa
     1621 cacagcggga gagattacga gtcgttccag aaaatgtata atattgagga aagaaaagat
     1681 tccctgatga tggaattggt tcaaaaaaag gtatttgact ttattgaaca gaatgcaaat
     1741 attaaggtcg tggaaaaagt cggactgaat acggaggcga aatcgtgacg ttgattccta
     1801 tggtggttga acaggatggc cggggcgaac gggcatacga tatctattcc cgtttgttga
     1861 aagaccgtat cgtatttctc ggtacggcga tcgacgacaa tgtggccaat ctaattgtcg
     1921 ctcaaatgct ttatctggaa gccgaaaatc cggacaagga aattttcttc tatctgaatt
     1981 caccgggagg cattgtcagt gccgggatgg ccatttatga tacgatgcgc tacatcaaat
     2041 caccgatttc tacggtatgc atgggacagg cggccagtat ggcagccctc cttctggcgg
     2101 cgggagaaag aaaaaagaga ttcgcactgc cgcattcgcg gattttaatc catcagcctc
     2161 tgggcggttt tcagggacag gcaacggata ttgacattca ggcaaaagaa atattaagat
     2221 taaaggacga gttgaacaaa atcctggccg atttgacaca acagccgctg gctaaaatag
     2281 tcaatgatac ggagcgggat tattttatga gcagtgaaca ggccaaagaa tacggtttaa
     2341 ttgatgaaat cattatcaga aagccggatg cttcagccgt cagggccaag gagaaataag
     2401 tgataaaaaa aggcaagaat aacagaatgg atgacggaat gcttttttgt tccttttgcg
     2461 ggaaaatgca aagcgaggta cgcaagctcg tagcggggcc gacagcctat atttgcgacg
     2521 agtgcattga actctgccgt tatattgttg aagaagaaga cgataagctg attgaaaaag
     2581 acgctcaaaa agccttgccg tatccgaaag agattaaaaa atttctggat cagtacgtca
     2641 tcggtcagga aaaaacaaaa aagattttgt cggttgccgt ctataaccat tacaaacgac
     2701 ttttttcgcg cgtcgaatcc gacggtatag aaattcagaa aagcaatgtg ctgctgatcg
     2761 gtcccaccgg ctccggtaaa accttgctgg ccaagactct ggccaagttt ctgaatgtcc
     2821 cgttcaccat tgccgacgca actaccctga ccgaagcggg ttatgtcggc gaggatgtgg
     2881 aaaatattat tttaagtctg ctccaaaatg cggattacga tgtcgcccgt gcgtcgaagg
     2941 gaatcgtata cattgacgag atcgataaaa tcgcgaagaa gtcgggaaat ccgtccatca
     3001 cacgcgacgt atccggcgaa ggagtgcagc aggccctttt gaagatcatg gaagggacca
     3061 tggccaatat tccgcccaaa ggcggtcgca aacatcctca gcaggaattt atccaggtgg
     3121 acacgaccaa tattttgttt atctgcggcg gcgctttcaa tgatctggaa agcatcatct
     3181 ccaagaggct gggcgtcaac gccatggggt tcggcgcgga aattaaaagc aagaaagaaa
     3241 aacgcatcgg tgaactgttg agtgaagtcc gatcggaaga tttgctgaaa ttcggtctga
     3301 ttccggaatt catcggccgg ttgcccgtca ttgccactct ggatgaactg gatgaagccg
     3361 cgctgatcaa gattctggtc gagccgaaag atgccatcat caagcagtac aagaaattat
     3421 tcgcgctgga aaacgtggtt ttgaaattta ccgacggcgc cttgaaggcg gtggccagct
     3481 tatccacgga gagaaaatcc ggcgcccgcg ggttgcgttc gattctggaa aataccatgt
     3541 tggaagtgat gtatgacatt ccatcgcaaa ataatatcaa agaatgtgta atcaatgaag
     3601 acgtcgttct gaaaaaagaa aaaccaattt taatttacga atccaaatcg gaatccgctt
     3661 aaatgcagta aagggaaagt atgtttgatg aaaagataga aaacgaacag gaaaaaacgg
     3721 ccatcatccc tcttttgccg ttgagagatg ttgtcatgtt tcctcatatg attgttcctc
     3781 tgtttgtggg ccgtgagaaa tccattgccg cactggagtc ggcgatgaaa tacgaaaaga
     3841 gtattttcat ggtcgcgcag aagaacgcca aggatgacga tccgaagaaa gaagatattt
     3901 atcatgtcgg aaccatcggc atcattattc agcttttgcg cctgccggac ggcacggtga
     3961 aggtgctggt tgaaggcaag accagaggca tgatcaaaga ataccttccg aacagcgatt
     4021 tttttctggt gaaagtcaac gaagtggacg atattgacga cggcaacgac gtcaagaaac
     4081 aggccctgat gcgcagcatc aaagaatctt tcgaactgta cctcaagctc agcaaaaaaa
     4141 ttcacgtcga aatgctgggc acggttgtgg cgattgagga tgcttcaaag cttgccgatg
     4201 tggtcatcac tcatctcaat gtcaaactcg aagacaaaca aaaaatcatt gagatatttg
     4261 atatcagcga gagactggaa gcgatttatt ccctgatgct ttcggaaatt gaaattctgc
     4321 aggtcgagga aaagatcaag cgccgcgtca aaaaacaaat ggagaagacg cagaaggatt
     4381 actaccttaa cgagcagatg cgcgccatcc aaaaggaaat gggcgaaaaa gacgatttcc
     4441 gaaatgagat caacgagttg gaaaaacgcc tgaagcagaa gaaaatgtcc gaggaggcgg
     4501 tcaaaaaagt caagcaggaa ctcaaaaagc tgcaaatgat gacccccatg tccgcggaag
     4561 ccaccgttgt ccggaattac atcgactgga tactcgacat gccctggtcg gaaaaaacgg
     4621 agaacaaata cacgctgaaa gaatccgaag aaattctgga tgccgatcat ttcggattga
     4681 agaatgttaa ggagcgcatc atcgaatacc ttgccgttca atctctggtc aagaaaaaca
     4741 agggatctat tttatgtctt gtcggtcctc cgggtgtggg aaaaacatcc attgccaaat
     4801 cggtggccag ggcgacgaac agaaaatttg tccgtatttc tctgggtgga gttcacgatg
     4861 aggcggagat cagaggacac cgccggacct acatcggagc gatgcccgga aaaatcatcc
     4921 agcttttgaa aaaagccggt tccaataatc cggtcttttg tctggatgag gtggataaac
     4981 tcagctccga tttcagaggc gatccgtcgg ctgcgcttct ggaggtgctc gatccggagc
     5041 agaactttgc gttcaacgac aactatctgg atctggatta cgacctgtcg gaaatcatgt
     5101 ttattacgac ggcgaatgtt ttgcagacga ttcctgcgcc gcttcaggac agaatggaag
     5161 tgatccgcat tgccggatac acggaaccgg aaaaactgca gatcgcgaaa aaatatctga
     5221 ttgccaagca ggtggaagcc aacggcctgg tgatggagaa tattgaattt acacacggcg
     5281 ccttgttgaa aatcattcgc gaatatacac gcgaagccgg cgtccggaat cttgatcggg
     5341 aaatcgcttc gatctgccgc aaagtagcca aggaaatcgt caataacgat aaaaaaggaa
     5401 gggtggtcat cggatctaaa tcggtcggta agtatctggg tgtgcccaaa ttccatcacg
     5461 gtgaaataga agaaaagaat aaaatagggc tgacggtcgg tctggcctgg acggaggtcg
     5521 gcggagaact tctgtcgatc gaagcctcca tcatggaagg aaacggaaaa atgattatga
     5581 ccggcaaact tggcgatgtt atgcaggaat ccgtgcaggc ggcgttgagc tacatccggt
     5641 cgcgtgccga cgaattcggt ttgaccaaga atttctatag aaaaattgat attcacgttc
     5701 atgtgccgga gggtgctatt ccgaaagacg gtccttccgc cggtgtcgcc atgacgacgt
     5761 ccatcgcctc cgttttgttg cgcaaaaaag tgcgtgcaga tctggccatg accggggaaa
     5821 tcaccctgcg aggcagaatt ctgccggtcg gcggtatcaa ggaaaaggtt ctggcggctc
     5881 atcgcggcaa catcaaaacg gtcattatcc cgaaggacaa ccagcgggat ctggcggaag
     5941 tccctcagaa cgtgcaaaaa gctctgaaaa ttatctttgc ggaacatatt gatgatgttt
     6001 tgaaactggc tttcgttcag gacgagggag agatgagacc ggctgtcgat gccgacgttc
     6061 cgatgcgttc ccaatcggtc aattaaaaag cttttttaag cttttatgac ttgacaagaa
     6121 atatcaatgt tgc
//
LOCUS       DAOSME010000101         6079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_14214_length_6079_cov_28.865704, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000101 DAOSME010000000
VERSION     DAOSME010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14214_length_6079_cov_28.865704"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1836)
                     /locus_tag="PLG98_10575"
     CDS             complement(187..1836)
                     /locus_tag="PLG98_10575"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HOE33749.1"
                     /translation="MKGKSLNGSRYGFQLTIKELLNGPRLCAQHQEIVYRDRIRYTYK
                     DLFDRINRLGSALTGLGVRKGNTVAVFDYDSHRYLECFFAIPMMGAALFTVNWRLSPD
                     QIEYTINHAEAGVILIHTDFLPLLASIRGKLTSVKKIILLSDDETLPKTDLAIDGEYE
                     AILGAASDNFDFPDLDEETKATTFYTTGTTGLPKGVYFSHRQIVLHAMSVCIGVSGHD
                     SPLRFSTSDVYMPITPMFHVHAWGFPFIATMMGVKQVYPGKYEPEMLMKLIVGEKVTY
                     SHCVPTILQMIVSSPVAKKVNLSNWKVIIGGARLSKGLAMAGIDMGIQIMTGYGMSET
                     CPVISVSNLKPSMKNYDNEQKSDIAIKTGMPVALVDVRIASSDGNFLPNDGQATGEII
                     VRAPWFTKGYYKDREKTEELWQGGWLHTGDVGYMDHEGYIQITDRLKDVIKTGGEWVS
                     SLDLENAISLHGAVQEAAAVGIPDPKWGERPMMLVVLTPPFRGDSITPEVLKKHMKEC
                     ADQGKIPRYAIPDRYIITDNIPKTSVGKTDKRVIRTLYASV"
     gene            complement(1974..2666)
                     /locus_tag="PLG98_10580"
     CDS             complement(1974..2666)
                     /locus_tag="PLG98_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE33750.1"
                     /translation="MAASKTISTGKHRILIVDDHPVFRHGLAQLINQENDLFVCGEAE
                     DYQSALKAVKDLQPAMIIVDITLKDMSGIDLIKEIHKTRRDIAMLVISMHDESLYAER
                     ALRAGARGYIMKQEASESVVRAIREVRQGSIYASKKMTDQIFSRFIEGNKKLAESPLH
                     SLTDRELEVFQLIGEGLGISEIGTRLHLSVKTIGTYRERIKEKLNLKTSTELLRYAVN
                     WVENKKLSDTSP"
     gene            complement(2672..3778)
                     /locus_tag="PLG98_10585"
     CDS             complement(2672..3778)
                     /locus_tag="PLG98_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE33751.1"
                     /translation="MAKEVMTKAQLVEELQEAHRKIKRLRSQARKYKRIEDKLTRSEQ
                     FYRTLFENSGTATIVIEKDTTISMMNNDFSNFTGYPRNEILNHSWTLYVAEDDVDRLL
                     FYHRTRREGPTSAPRNYEFKIRNKDGKHLHVYMTIAIIPGTSQSIASMVDMTAIKKLE
                     KEVLDTSERERQKIGYELHDDLGQHLIGIDVMTKVLRDKLTPISDENARYAGEINHLV
                     REAIDKTRRLARGLCPVHLTSLGLEASLENLATSTSSIFNITCLFRCPRHIPIHDNTL
                     ATHLYYIAKESVHNAIEHGKSSRIIMELLNNSSSVSLSILDNGSGIKDIARATGIGLR
                     IMHYRARMIGAALRIESEPDGGTLVTCAFNLRKN"
     gene            complement(3802..4068)
                     /locus_tag="PLG98_10590"
     CDS             complement(3802..4068)
                     /locus_tag="PLG98_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33752.1"
                     /translation="MKIEIEILAKKAKGNRTFPVYLLYPTRCLEIKFHYENANLRNVR
                     AESFFAGRHPQAAVSSKRGKSIEIQLSNEEWVFPTSGVIFIWDV"
     gene            complement(4415..5620)
                     /locus_tag="PLG98_10595"
     CDS             complement(4415..5620)
                     /locus_tag="PLG98_10595"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE33753.1"
                     /translation="MTAAICIARVKCKDCGHEYLLAFSCKRRHFCPSCHQKRVVEFGE
                     WLCMDVLKKAPHRHFIFSLPKILRRYLLYDRKLLAGLSRCAWESLKVFMQQAVPEKAP
                     LPGAAIAIQIFGGFLGFNPHCHILLTDGCFYGDKGMFRVAPPLELKKLEMIFRHKVFR
                     MLLNKGKIKKEMIAMLSTWRHSGFNIFCGNRILPKDETAIENLARYIIRASFSQERMR
                     YLEKEGAVIYSSKDGRTSKTFPALDWLAAMCSHIPNRGEQMVRYYGYYSNVTRGRRKE
                     AGTVDAIPSMLEPAESNKALRKNRARLIQKIYEADPLVCPKCKGAMRIISFIEDQQVI
                     REILTHLELRLVRSRPPPAGASHADRPKICAATLLDSKASDSYTHTPHPNTDAYADPE
                     YSWDDYIQS"
BASE COUNT         1391 a         1439 c         1645 g         1604 t
ORIGIN      
        1 gacgcgacat taacgtttaa cggcagtaaa tgaagtgcct tcatcagtgc tcggacgggg
       61 ggcatggatt cgtttcattt tgcgccttta attttcaggc aggattgagc agagaggaga
      121 gcgggccttc agccggcccg ctcatggatc caggggtaca gacgggcaaa agttggattt
      181 atccatttac acgctggcat acagagtccg gatgactctt ttgtcggttt tcccgacgct
      241 ggtttttggt atattgtccg taataatata tcgatcgggg atggcatatc gggggatttt
      301 cccttgatcg gcgcattcct tcatgtgttt tttcaggacc tccggtgtaa tgctgtcgcc
      361 acggaacggc ggggtcagaa caacgagcat catgggcctt tctccccatt tgggatcggg
      421 aatgccgacg gctgccgcct cctgcaccgc gccgtggaga ctgatggcat tttccaggtc
      481 aagggatgag acccattcgc cgccggtttt aatgacatcc ttaagcctgt ccgtgatctg
      541 aatgtaccct tcgtgatcca tataccccac atcgcccgta tggagccagc cgccttgcca
      601 gagttcttcc gtcttttccc gatctttgta atagcctttg gtgaaccagg gagcgcgaac
      661 aataatttcg cccgtggcct ggccgtcatt gggaaggaaa ttaccgtcgg aagaagcaat
      721 gcgcacatcg acaagagcga caggcattcc tgtcttgatg gcaatatcgc ttttctgttc
      781 gttgtcataa tttttcatgg atggtttcag gttggatacg ctgatgaccg ggcaggtttc
      841 cgacatgccg taccctgtca taatttgaat ccccatatcg atgcctgcca tggccaaccc
      901 cttggaaaga cgggctccgc cgatgatcac cttccagttg gaaagattaa cttttttggc
      961 aacgggactg gatacaatca tctgcaggat cgtgggaacg cagtgggaat aggtgacttt
     1021 ttcgccgacg atgagcttca tcagcatttc cggttcgtac ttgccgggat aaacctgttt
     1081 gacacccatc atggtggcga tgaagggaaa cccccaggca tggacatgaa acataggagt
     1141 gatcggcata tagacatctg aagtagagaa tctgagcgga ctgtcatgcc cgctgacgcc
     1201 gatgcagacg ctcatggcgt ggagcacaat ctggcgatga gagaagtaca cgcctttcgg
     1261 aaggcccgtt gttccggttg tatagaatgt agtggctttg gtctcttcat caagatcagg
     1321 aaaatcaaag ttgtctgacg ccgcgcccag aatcgcttcg tattcgccat cgatggccaa
     1381 atctgttttg ggtaatgttt catcgtccga aagcagaatg attttcttta cggacgtaag
     1441 ttttccccgg atggaagcga gcaggggaag gaaatcagta tgaatgagga tgacgcccgc
     1501 ttccgcgtgg ttgatggtat attcgatctg gtcaggagac aggcgccagt tgaccgtaaa
     1561 cagcgcggct cccatcatgg gaatggcaaa aaagcattcc aggtagcggt gggagtcata
     1621 gtcgaagacg gcgaccgtgt tcccctttct gacgccaagt cccgtgaggg cgcttcccag
     1681 tcggttgatc cgatcaaaca ggtctttata agtatagcga atcctgtccc ggtatacaat
     1741 ttcctggtgt tgtgcgcaaa gccttggccc gttgagcagt tctttaatgg tcagttgaaa
     1801 cccgtaacga cttccattca aagatttccc cttcatattg ccctcctttg gttgcagttt
     1861 cctctggaaa gcatgatcgt ctaaagcggg aatggaaaca atccgcagga agctgttttg
     1921 tctgtgcttg aatggatgaa aaaaaagtag gccgatgacc gacaaaaaaa ggctcagggg
     1981 gatgtgtcgg ataatttctt gttctcaacc caattgacgg cgtaacgaag aagctcggtg
     2041 ctggttttga ggttcagttt ttccttgatc cgttcccggt aggttccaat ggtttttacg
     2101 ctgagatgga gccgggtgcc gatttcgctg atgccgagtc cctctccgat gagttggaaa
     2161 acctcaagct cccggtctgt cagggaatgg aggggcgatt cggcgagttt tttattgccc
     2221 tcaatgaagc gggaaaagat ctggtccgtc atttttttgc tcgcatagat gcttccttgc
     2281 cgcacctcgc ggattgcccg gacaaccgat tcggaagcct cctgtttcat gatgtacccc
     2341 ctggcgccgg cgcggagagc gcgttcggcg taaagggatt catcgtgcat ggagatgacc
     2401 agcatagcta tatcccggcg ggttttgtga atttccttga tgagatcaat gccgctcatg
     2461 tccttgaggg tgatatccac gatgatcatg gccggttgca ggtccttgac ggcctttagg
     2521 gcgctttgat aatcttccgc ttccccgcag acgaacagat cgttttcctg gttgatgagc
     2581 tgcgcaagac catgcctgaa aacgggatga tcatcgacaa tgaggattct gtgtttgccg
     2641 gttgagatgg ttttgcttgc ggccattttc tttagttctt ccttaaattg aaggcgcagg
     2701 tgaccagggt cccgccatcc ggttccgatt caatgcgcag cgctgccccg atcatgcggg
     2761 cccgataatg catgattcgc aatccaatgc cggtggctct ggcgatgtcc ttaattccgc
     2821 tgccgttgtc aagaattgac agggagacgc tgctgctgtt gttcaaaagc tccatgatga
     2881 ttctggagga tttgccgtgt tcgattgcat tgtgaacgga ttccttcgcg atataataaa
     2941 ggtgggtcgc cagggtattg tcgtggatgg gaatgtgccg ggggcagcgg aagaggcagg
     3001 tgatgttaaa aatggatgat gtcgaagtgg caagattttc cagcgaggcc tcaagaccca
     3061 gggaggtgag atggacggga caaagtcccc gggccaaccg acgcgtcttg tctatggctt
     3121 ccctgacaag atggttgatc tcgcccgcat aacgagcatt ttcatcagaa atcggggtaa
     3181 gcttgtcccg caggactttg gtcatcacat caatgccgat caggtgctgg ccgagatcgt
     3241 catgaagttc gtatccgatc ttttggcgct cccgttccga tgtgtcaaga acttcctttt
     3301 ccagtttctt gatcgctgtc atgtccacca tcgaggcaat gctttgtgag gttccgggaa
     3361 tgatggcaat ggtcatgtaa acatgcagat gtttgccgtc cttgttccgg attttgaatt
     3421 cataatttct gggtgccgag gttggaccct cacgcctcgt tctgtggtag aataacagac
     3481 gatcaacgtc atcttcagcc acatacaggg tccaggaatg attaaggatc tcattacggg
     3541 ggtacccggt gaaattggaa aaatcattgt tcatcatgga gatggttgtg tctttttcga
     3601 tgacgatggt cgctgttccg gaattctcaa acaatgttcg gtaaaactgc tccgagcgcg
     3661 tcagtttatc ctcaatcctt ttatacttgc gggcctgtga acgaagcctt ttgattttcc
     3721 tgtgcgcctc ctgcagttcc tccacaagct gcgcttttgt catgacttcc tttgccatcg
     3781 cttttcctcc ggttctctcc cctatacgtc ccagatgaaa atgacgccgc tcgttgggaa
     3841 tacccactct tcgttggaaa gttgaatttc gatggatttg ccgcgcttag aggaaacggc
     3901 tgcctgcggg tgacgccccg caaagaagct ttcggcccgg acattcctga gattcgcgtt
     3961 ttcgtaatgg aatttaattt caaggcagcg tgtcggatac agaaggtaaa cagggaacgt
     4021 ccggttgcct ttggcttttt tggcgagtat ttcaatttca attttcactt ccctgttgat
     4081 tttcggcttg agcttttcgc tgccgaacca gacttcatat ccacgatggg ttttctttgc
     4141 ttcaatgatc ggaatatcct ccccgtcgat tgattgattg attgatcaga acttgctttt
     4201 agttgtcggc agtataagaa acgttcactt gtgtcaagaa gatattgggt acaagatata
     4261 gggtgtgcac acaatgaccg ttttggggat gcgtcgaggg agttaacttt taaacctgcc
     4321 tgcctgttta tccgccgtgg tagatcattt acaccggaat tcagacagac ctctccggtg
     4381 cgccttaaat aattcttgaa aaaagcacaa cgcgttatga ctggatgtaa tcatcccagg
     4441 aatattccgg atcggcatag gcgtcagtgt tcgggtgcgg ggtgtgcgtg taagaatcgg
     4501 aggctttgga atccagtaat gtggcggcac agattttggg cctgtctgcg tgcgacgcac
     4561 cggcaggcgg gggccttgaa cgcacaagcc ggagttccag gtgcgtgaga atctcccgga
     4621 tgacctgctg atcttcaata aagctgatga tgcgcatggc ccccttgcac ttgggacaaa
     4681 ccaaagggtc agcttcatat atcttctgaa tcaaccgtgc ccggtttttc cggagagctt
     4741 tattgctttc cgccggttcg agcatggagg gaatggcatc aaccgtgccc gcctctttcc
     4801 gtctaccccg cgtgacgttg ctataatagc cgtagtagcg caccatctgt tcgccgcggt
     4861 tggggatatg ggaacacatg gccgccagcc agtccaacgc ggggaatgtc ttgcttgtcc
     4921 tgccatcttt ggatgaatag atgaccgcgc cttccttttc cagatagcgc atccgttcct
     4981 gcgagaacga cgcccggatg atgtaacgcg ccagattctc tatggcggtt tcatctttcg
     5041 gcaaaatccg gttgccgcag aatatgttga agccggaatg ccgccatgtc gagagcatgg
     5101 cgatcatttc tttcttgatc tttcccttgt tcaggagcat ccggaaaacc ttgtgccgaa
     5161 aaatcatctc caactttttg agttccagcg gcggggcgac acgaaacatc cctttgtcgc
     5221 catagaagca gccgtcggtc aggagaatat ggcagtgcgg attaaaaccc aaaaagccgc
     5281 cgaagatctg gatagcgatg gcggcgcccg gcagaggcgc tttttcgggc acggcttgtt
     5341 gcatgaaaac cttcagggat tcccaggcgc agcgactgag tccggcgaga agttttctgt
     5401 cataaagaag ataccggcga aggattttgg ggaggctgaa gatgaaatgc cggtggggcg
     5461 ccttcttgag cacatccatg cagagccact ccccgaattc cacgacgcgc ttttgatggc
     5521 aggaagggca gaagtggcgg cgcttgcagg aaaaagccaa caggtattcg tggccgcagt
     5581 ctttgcattt gacacgggct atgcagatcg ccgcagtcaa gataacggta gatcaccttt
     5641 tcgatataag gccgccagaa gccgtactgt gtggagaagt gttcgtcata ggattgaacg
     5701 aaggtttcaa aatagtcctc cacgcagtgg taataatccg aatcttgcgg ttgtcggggc
     5761 tggtatgagg ctgccggcaa tgtcatatcg tcctcactgg ataggataaa tgaactgcga
     5821 aggagtgatt tatagtagaa cgaaagttct attgtcaaca gaaaaataat gatacccatt
     5881 tcatgaacaa atgtttgaca gttatgccgg atttggataa agattgctag tcataaaata
     5941 ggccgtgtaa taaaccggcc aaacactgtt aaaagacgca gagattctgc aaaaaaaata
     6001 tgagaagagg aattcaatat ggctcacagt aattggttgt atcagacgcg ggacatcatg
     6061 ttccagatca aagaatggc
//
LOCUS       DAOSME010000102         5696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_15503_length_5696_cov_17.514802, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000102 DAOSME010000000
VERSION     DAOSME010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15503_length_5696_cov_17.514802"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..3397
                     /locus_tag="PLG98_10600"
     CDS             347..3397
                     /locus_tag="PLG98_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE33754.1"
                     /translation="MENIFFEKPILNSPYEYPTRHWELDTQGQPTQQIKEKRRRAEYI
                     TPIPKPKKRRGSASQQDIIFDEGKGLSSQEQQYDPTPVINNLRIHVDKWRSIPNPNDW
                     KVTPETSRLLQHWRKHKFSAYRPFFCQVEAVETVIWLTEVAPTEKAGKDFLAHLENAN
                     HYANPDLMRLALKLATGAGKTTVMAMLIAWQTINAVRHPGSKKFTRGFLVVTPGITIK
                     DRLRVLQPNDPDSYYKNRELVPNDMLRELERAKIVITNYHAFKLRDRLELSKGGRSLL
                     QGRGGEELKTLETEGQMLQRVMPGLMGMKNVLIINDEAHHCYREKPDTEEDEDLKGDD
                     KKEAESNNEAARLWINGIETVTRKIGATQVIDLSATPFFLRGSGYAEGTLFPWTMSDF
                     SLMDAIECGIVKLPRVPVADNIPGGDMPMFRNLWEHIRKDMPKKGRGKASGLDPLKLP
                     TRLQTALEALYGHYEKTYNLWKESGMKLPPCFIVVCNNTSTSKLVYDYISGFEYPPKE
                     NGVTPPPYLGKFSLFSNYDEYGNPLARPRTLLIDSEQLESGEALDDNFRTVAKEEIER
                     YRREIIERTGDRSQAENLTDQDLLREVMNTVGKKDSLGDSIRCVVSVSMLTEGWDANT
                     VTHVLGVRAFGTQLLCEQVIGRALRRQSYDLNEDNLFNVEYADVLGIPFDFTAKPVVA
                     PPQPPRETIRVKAVRPERDPLEIRFPRVAGYRVELPEEKITASFTKDSELDLTPDLVG
                     PTETRNQGIIGEGVDLNLIHTGDLRMSTLIFHLTTRLINTKYRDPGNEPKWHLFGKLK
                     RITKDWLDRCLKCKGGTYPAQLMYQELADMACERITAAIMSSLEGAHPIVAVLDPYNP
                     VGSTIHVNFNTSRTDRWETDARRCHINWVILDSDWEAEFCRVAEAHPKVKAYVKNHNL
                     GLEVPYRYASETRIYLPDFIVQIDDGHGEKDLLNLIVEIKGYRREDAKEKKNTMDTYW
                     VPGVNNNGQYGRWAFAEFTEIYQIESDFKAKVAKEFDKMIEKII"
     gene            3419..4594
                     /locus_tag="PLG98_10605"
     CDS             3419..4594
                     /locus_tag="PLG98_10605"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE33755.1"
                     /translation="MKKIQKKTVLDLHLKGYQEFLSDLKKRIRDAQVKATLSVNRELI
                     LLYWEIGCCLDAVQKKQGWGAAVIPRLAKDLKNELPELKGFSERNISRMLAFFREYPE
                     LKAFLPQAVAKKKPSNILPQPVAEIPVSETNLSRTMESLLLALPWGHNILLIEKVKDC
                     DLRLWYAQKTLENGWSRNVLDLMIQDSAHERQGKAITNFDRSLPPGQSVLAKQTLKDP
                     YIFDFLTIEQPFHERELETGLIRHLERFLLELGAGFAFVGRQVHLEVGDDDFYIDLLF
                     YHLKLRCYVVIELKSGKFKADYAGKMNFYLNVVDDRFRHISDQPSIGLILCQDKNKIL
                     AEYALHGMNKAIGVSQYKLTRALPKEFKTSLPSIAQIEAELTEISAQTTKKNEKGEE"
     gene            5095..>5696
                     /locus_tag="PLG98_10610"
     CDS             5095..>5696
                     /locus_tag="PLG98_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33756.1"
                     /translation="MQNKKNGKTIEALTHTDARRRNIPTAEFQSVVQKEANDPLTVAY
                     PRNAEGLDEEKTNRNRDLDPQLVWRGKDEQDWSDLVVHVPPVYIQEKVHPKVLIDDLL
                     KRTASDKQAAEVQTDLFADFNGVPTEAAKTEFYQHDQNWSNRMILGDSLQVMASLAER
                     EGLRGKVQCIYFDPPYGIKFNSNFQWSTTSRDVKDGNVEH"
BASE COUNT         1603 a         1388 c         1420 g         1285 t
ORIGIN      
        1 cacttccaat ttaagggact ataaaaaaat cgagggattg aagatagaag aaaagaatta
       61 aagcgggttt ggcggccggg actggaaatc gtttctgatt ggcaacgctt ttttgcgcga
      121 ttctctttgc cggttcattc ttgcagcttg aattgaaaag cttcggagta atcacgatga
      181 ttgctccagc aggaggccgg acaggtatga cgaatcataa ttttttacca ttatttcgtt
      241 gacatacgga tacgttcttc ctatactctg cctcaccatt agtaaaatct ggactgacat
      301 ttagctaccg catcactgct tcctctctca ggataaggaa atgaacatgg aaaacatctt
      361 ttttgaaaag cctattctga attccccata tgaatatcca acccgccact gggagctcga
      421 tacccagggg cagccgacgc agcaaattaa agaaaaacgc cgacgcgctg aatacatcac
      481 gccgatacca aaacctaaaa aaaggagagg ttctgcatcc cagcaggata ttatctttga
      541 tgaaggcaag ggtttgtcat ctcaagagca gcagtatgat ccaacgccgg tcatcaataa
      601 cctccgcatc cacgtggata agtggcgcag cattccaaat cccaacgact ggaaagtgac
      661 gcctgaaaca tcccggttgc tgcagcactg gcgcaaacac aaattcagcg cctatcgacc
      721 gtttttctgt caggtcgagg ctgtggaaac cgttatctgg ttgaccgaag tcgcgcccac
      781 ggagaaagcc ggcaaagatt ttctggccca cctggagaat gccaaccatt acgccaatcc
      841 cgacctgatg aggttagcgc tcaaactggc gaccggcgcc ggcaaaacca ccgttatggc
      901 catgttgatt gcctggcaga ccattaacgc cgtgcggcat ccgggaagca agaagtttac
      961 gcggggcttt ctggttgtca cgccggggat cacgattaaa gaccgtctgc gcgtgcttca
     1021 acccaatgat ccggacagct actacaaaaa ccgggagctg gttcccaatg acatgctccg
     1081 cgagctggaa cgcgctaaaa ttgtcataac gaattaccac gcctttaaac tgcgggaccg
     1141 gctggagctt tccaaaggag gacgctcatt gctgcaaggc agaggcggcg aagaactcaa
     1201 gacgctggaa accgaaggcc agatgcttca gcgcgtgatg cctggtctga tggggatgaa
     1261 aaatgtcctg atcatcaacg acgaagcgca ccactgttat cgcgaaaagc cggatacaga
     1321 ggaagatgaa gacctcaagg gcgacgacaa aaaggaagcc gaaagcaaca acgaagccgc
     1381 ccggctctgg atcaacggca tcgaaaccgt tacccgcaaa atcggtgcga cacaggtgat
     1441 tgatttatct gcaacgcctt ttttccttcg gggctccgga tatgccgaag gcacactgtt
     1501 tccctggacg atgagtgatt tctcgctgat ggacgccatc gaatgcggca tcgtcaaatt
     1561 accgcgtgtt ccggtagctg ataatatccc gggcggcgat atgccgatgt ttcggaacct
     1621 gtgggaacat atccgcaagg acatgcccaa aaagggcagg ggcaaggctt ccggacttga
     1681 cccgctgaaa cttccgacac gtcttcagac cgctctggag gccctttacg gccactacga
     1741 gaaaacgtat aacctctgga aagaaagcgg catgaagttg ccgccgtgtt ttattgtcgt
     1801 ctgcaacaat acatccacgt ccaaactggt ctatgactat atatccggct ttgagtatcc
     1861 acctaaagaa aacggcgtga cgcccccgcc ttatttgggc aaattctccc tgttttccaa
     1921 ttacgatgaa tacggcaacc cgctggccag gccgcgaacg ctcttgattg acagcgagca
     1981 actggaatcg ggtgaagcac tggatgacaa ttttcgaaca gtggccaaag aagaaattga
     2041 acgctatcgc cgtgaaatta ttgagcgcac cggcgaccgc agccaggcgg agaatctgac
     2101 cgaccaggac ttgcttcggg aagtcatgaa tacggtcggc aaaaaagaca gcctgggcga
     2161 ttctatccgc tgcgttgttt ctgtttccat gctgaccgag ggctgggacg ccaacaccgt
     2221 tacgcacgtt ctgggcgtcc gcgctttcgg cacgcagctt ttatgcgaac aggtgatcgg
     2281 ccgggcgttg cgccgtcaat cctatgattt aaatgaagat aatctattta atgtcgaata
     2341 tgccgatgtt ctgggcatcc cgtttgattt caccgccaaa cctgtggtag cgccgcccca
     2401 gccgccacgg gaaaccattc gtgttaaagc cgtccgcccg gagcgcgacc ctctggaaat
     2461 tcgcttcccc cgtgtggccg gttaccgtgt ggagctgccg gaagaaaaaa taacggcatc
     2521 gttcaccaaa gattccgaac tggatttaac gcctgatctc gtcggcccga ccgaaactcg
     2581 caatcagggc attatcggcg aaggcgtaga tttaaatttg atccacaccg gcgacctgcg
     2641 catgtcaaca ctcatctttc atctgactac ccgtttgatt aatacaaaat atcgcgatcc
     2701 cggcaatgaa cccaaatggc atcttttcgg gaaattgaaa cgcatcacga aagactggct
     2761 ggatcgctgc ctgaaatgca aaggaggaac ttatccggcg caactcatgt atcaggaact
     2821 ggccgatatg gcctgcgaaa ggattactgc cgcaattatg agttcgctgg agggcgcgca
     2881 tcctattgtt gccgttcttg atccttacaa tccagtcggc tccaccatcc acgtcaattt
     2941 caatacctcc aggactgacc gctgggaaac cgacgcccgg cgctgtcata tcaactgggt
     3001 cattctggac agcgactggg aagcggaatt ctgccgggtg gcggaagcgc atccgaaagt
     3061 aaaagcttac gttaaaaatc ataatcttgg tctggaagtg ccatatcgct acgcttcgga
     3121 aacacgcatc taccttcccg attttatcgt ccagattgat gatggccacg gtgaaaaaga
     3181 cttgctcaat ctgattgtgg aaatcaaagg ttaccgaaga gaagacgcca aggaaaagaa
     3241 aaacacaatg gacacctact gggttcccgg agtcaacaat aacggccaat atggccgctg
     3301 ggcctttgcc gaatttacgg aaatctatca aatcgaatcc gactttaagg cgaaagtggc
     3361 gaaagagttt gacaagatga tcgagaaaat tatttgataa agttggtatt tggagatgat
     3421 gaaaaaaata caaaagaaaa cggtgttgga cctccacctc aaaggatacc aggaatttct
     3481 cagcgatctc aaaaaacgta ttcgtgatgc ccaggtaaag gcaaccttgt cagttaaccg
     3541 ggaattgatt ctactatact gggaaatcgg ttgttgcctg gatgctgtac aaaaaaaaca
     3601 gggatggggc gcagcggtta ttcctcgtct ggcaaaagac cttaagaatg agttgcctga
     3661 actcaaagga ttttcggaac gcaacatcag tagaatgctc gcatttttca gggagtatcc
     3721 tgaactgaaa gcctttttgc cacaggctgt ggcaaaaaag aagccctcta atattttgcc
     3781 gcaacccgtg gcagaaattc cggtttccga gaccaatctt tcccggacga tggaatcctt
     3841 attactggcg ctgccttggg gacacaacat cctgcttata gaaaaggtta aggattgcga
     3901 cctgcgtcta tggtatgcac aaaaaacact tgaaaacggc tggagcagaa atgtcctgga
     3961 tctaatgatt caagacagcg ctcatgaaag acagggaaag gcgattacca acttcgaccg
     4021 aagtttgccg cccggtcaat cggttttggc taaacaaacg ttaaaagatc cttatatttt
     4081 tgatttttta acaatcgaac agcctttcca cgaacgcgaa ctggaaaccg gactcattcg
     4141 gcatctggaa agatttcttc tggaacttgg cgcaggcttt gcctttgtcg gcaggcaagt
     4201 tcatctggaa gtaggcgatg atgatttcta tatcgatctg cttttctatc atcttaaatt
     4261 gcgttgttat gtggtcatcg aattaaaatc gggaaaattc aaagccgatt atgccggtaa
     4321 gatgaacttt tacctcaatg ttgtggatga ccgtttcagg catatatcgg atcaacccag
     4381 tatcggttta attttatgcc aagacaaaaa caagattctt gcggaatatg cactgcacgg
     4441 tatgaataaa gccattggcg tgtcgcaata taaactgacg cgcgctttgc caaaagagtt
     4501 caaaaccagt ctgccgtcta tcgcacagat cgaagcggaa ctcacagaaa taagtgcgca
     4561 gactacaaaa aagaatgaga agggtgaaga gtgaagggtg aaaaataaat ggagcagccg
     4621 aggcgagatt tgccggaacg cacgtttgaa tttgcgcggc gcattgtcac attttgccgg
     4681 aaaatggaac aaaagccggg ttcaagccgt ctattggcaa accagctatt aagggcagga
     4741 acgtccatcg gggccaatgt gcaggaaggt caggcaggcc agagccgggc cgatttcatt
     4801 tctaaatatt ccattgcctg taaggaagcg cgtgaaacgc attactggct aaggctaata
     4861 gcatctcaac ggatgggcgc gaagatgccg aattgcagtc aatgattgaa gaagcaaacc
     4921 agcttgtggc tatcttgacc gttatagtga agaaatgccg agagaaaaag gacaaatgca
     4981 aagagtgaag tcagaattga gaagagtgat gtaaggagga attttctctt ttttcttttt
     5041 cactcttcac tctttaaact tcacacttct gcgacgagag gactatggaa agtgatgcaa
     5101 aataagaaaa acggtaaaac aatagaggca ctgactcaca ctgatgcacg tcgcaggaat
     5161 attcccactg ccgagtttca gtctgttgtg caaaaagaag cgaatgaccc gttgaccgtg
     5221 gcctatccgc gcaatgctga aggcctggat gaagaaaaaa caaaccgcaa ccgtgacctg
     5281 gacccgcaac tggtctggcg cggcaaagac gagcaggact ggtcggactt ggtggttcat
     5341 gtgccgccgg tttacattca ggaaaaagtt catcccaaag tgctcatcga cgacctgctg
     5401 aagcgtaccg catccgataa acaggcggcg gaagttcaaa ccgacctctt tgccgatttc
     5461 aacggcgtcc ccacggaggc cgccaagact gaattttatc agcacgacca gaactggtcc
     5521 aaccggatga ttctggggga cagccttcag gtcatggcgt ctttggccga acgcgaaggc
     5581 ctgcgcggca aagtgcaatg catttatttt gacccgccct acggcatcaa gttcaactcc
     5641 aacttccagt ggtccaccac cagccgcgat gtaaaagacg gcaatgtcga gcacat
//
LOCUS       DAOSME010000103         5638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_15724_length_5638_cov_19.993015, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000103 DAOSME010000000
VERSION     DAOSME010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15724_length_5638_cov_19.993015"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1301
                     /gene="galE"
                     /locus_tag="PLG98_10615"
     CDS             237..1301
                     /gene="galE"
                     /locus_tag="PLG98_10615"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOE33757.1"
                     /translation="MTEVLVTGGTGYIGSHTAVSLMEAGYRPVIVDNLSNSEASVVKC
                     IARITGKQPPFYKLDLRNANALNKVFEKHRIDSVIHFAGLKAVGESVDAPLLYYENNI
                     MGALSLCRVMAANRVKKIIFSSSASVYGEANIIPVTEDCPPAPTNPYARTKLMIEQIL
                     QDSHRADPAWRIILLRYFNPVGAHFSGLIGESPRGIPNNLMPFLSQVAVGRRPHINVF
                     GNDYPTPDGTGVRDYIHIMDLAEGHIAALKKIEKAAAPKGQKPLIVNLGTGKGYSVLE
                     MIRAFEKSSGQKIPYRIARRRPGDVATLYADTQLARKALGWKAKRNLADMCTDAWRWQ
                     SSHPEGYAGSSARPVKKNPG"
     gene            1372..2871
                     /locus_tag="PLG98_10620"
     CDS             1372..2871
                     /locus_tag="PLG98_10620"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOE33758.1"
                     /translation="MEAFSTLLLLFVCSLFLSLVLTPAVRALGIRLGAMDIPMERKMH
                     TEPIPRIGGLAVFLSFTITGMLFNFFHPFMGDLYVLNFSSFMGHLGAVVILSCGLWDD
                     FRRLNPWTKLLVQILAATCAFMGGATINSFLIGGVGFEFNTYGSYAITVFWFLLFINA
                     INLIDGLDGLAGGLIVFTCIVMTVSTFMHQDYLSAFYFAVLGGSVLGFLKYNFNPATI
                     FLGDGGSYFLGYTVAILAIRSSAKSHVGILMLIPLLALGIPIFDSILSPIRRFMLGKP
                     IFHPDKGHIHHIMMKMGLSSQRVVYMIYGITMALCILAIMIIILRDQTFEGLVLGGLL
                     LGMLILVRKLGYFEYLALDKFQGWFQDVTDVAGFSQNRRSFLALQIATSKSKNMDELW
                     HNIVEAMEMLKFHHAKLMVIDEPVREWTMPEDGEHAYFCAPSSEADLDGMLRFEIPLR
                     EYGSDTFMGKLILIKDLKKGFLKSYTIRRVEHLRRSLIPVLKKLKLKDG"
     assembly_gap    2875..2974
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3274..3618
                     /locus_tag="PLG98_10625"
     CDS             3274..3618
                     /locus_tag="PLG98_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33759.1"
                     /translation="MEIINRLLEEELKRLGTLHDFYEKKIMESPRGSLSVKARGKNRY
                     LYLARREDKKVVFEYVGKDVAYVRHALNEQMKQRREYQAKLREVKENIKEVKRSLRTK
                     RNRDGPAAVNSS"
     gene            3766..5043
                     /locus_tag="PLG98_10630"
     CDS             3766..5043
                     /locus_tag="PLG98_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE33760.1"
                     /translation="MKKFIFLSIILSLFAVFSVAQAEVRDRSFSLTPYVGGYFYEGNQ
                     DLKDSALFGLRAGYNFTRHFGIEGFASYTQTEIDDSYYSGTSPWQNIYNFGVEAIYHF
                     LPEKRFVPFVAMGLGGVHYEEARNYKSHYGAPDYGKIFEATKLAVDYGAGMKYFVTDN
                     IALRLDVRHILPLNGRWNNPHYIHNELQATFGVNFAFGGGPKVAVQEAAVEEAPVQPV
                     VSAADGDKDGVPDFRDKCPKTPAGRTVDADGCMPDSDGDGILDYEDHCANTPAGVKVD
                     ADGCPSDSDFDGVSDYRDKCPGTPHGSPVDEDGCLFKKTSMFLKIEFDSGKSVIKPRY
                     HQEIKRVADFMKKNSKATATIVGHTDNTGGRLANIRLSQNRADSVRQYLISKFGIAAQ
                     RIKAMGYGPDRPIAGNKTDAGRKKNRRVVASFE"
BASE COUNT         1340 a         1246 c         1484 g         1468 t
ORIGIN      
        1 tggataccgg cctacgccgg tatgacagac tgggtggatg ccggcctcgg catgacaaac
       61 atcgggccca ttcgggaatg acaaaagaat ctgtcattcc aggatgctcc tctacatgac
      121 atatttatat tttatttaaa aagacacgtt atgttgcttg ttaaaatgtg atatgtacac
      181 tcattgcttt aaaataatct aaaacattca aagtcaaatc tgtaaggatc atgttgatga
      241 cggaagtgct ggtcacagga ggtacaggat acatcggaag ccatacggcg gtgtcgttga
      301 tggaggccgg atatcggccg gtcatcgtgg ataatctttc caacagcgag gcgtcggttg
      361 tcaaatgcat cgcccgcatc acgggaaagc aaccgccttt ttataaactg gacctgcgca
      421 acgccaacgc gctcaacaag gtttttgaaa agcacaggat cgattcggtc attcattttg
      481 ccggtctgaa ggcggtgggt gaatcggttg acgctcccct gctctattac gaaaataata
      541 ttatgggcgc gctgtcgctg tgccgggtca tggcggccaa ccgggtgaag aaaattattt
      601 tcagttcgtc cgcgtcggtt tacggggaag caaatattat tccggtgacg gaagactgtc
      661 cgcctgcccc gaccaatccg tacgcccgca ccaagctgat gatcgaacag attctgcagg
      721 atagtcaccg ggcggatccc gcgtggcgga ttattctttt gcgctatttc aatcctgtgg
      781 gtgcgcattt cagcgggctg atcggcgagt ccccccgggg aatcccgaat aatctgatgc
      841 cgtttctcag tcaggtggcc gtcggccgcc gtccgcacat taatgttttc ggcaatgatt
      901 atccgacgcc tgacggcacc ggcgtccgcg actatattca tattatggat ctggcggaag
      961 gccatattgc cgcattgaaa aaaatagaaa aggcggcggc gcccaaagga caaaagccgc
     1021 tgattgtgaa tctcggaacg ggcaaagggt attccgttct ggagatgatc cgggcgtttg
     1081 aaaaatcgtc cggtcaaaaa ataccgtatc gcatcgcccg gcggcggccc ggcgatgtgg
     1141 caacgcttta tgccgatact caactggcgc gcaaggcgct gggctggaag gcgaaaagaa
     1201 atttggcgga tatgtgcacc gatgcgtggc gctggcagtc ttcgcatccc gaagggtatg
     1261 ccggttcctc cgcacgcccg gtgaaaaaaa atccggggta aagttgaagc ggcaatgttt
     1321 gccgtaaatg taacagaaaa agtataagat tcgatgtagg tgtgtggcag tatggaagct
     1381 ttttcgacat tgttgttgtt gtttgtgtgt tctttgtttc tttctcttgt tttaacgcct
     1441 gcggtgcgag cgctggggat ccggctgggc gcgatggata ttcccatgga gcgcaagatg
     1501 cataccgaac cgataccgcg catcggcggc ctggctgttt ttctttcctt caccatcacg
     1561 ggcatgctgt tcaatttctt ccacccgttc atgggagatt tatacgtgct gaatttcagt
     1621 tccttcatgg gacatctggg cgccgttgtg attttgagtt gcggcctctg ggacgatttc
     1681 cggcgtctta atccctggac gaaattattg gttcaaatcc tggcggcaac atgcgccttt
     1741 atgggcggcg caacaatcaa tagttttttg atcggaggcg tcggcttcga gtttaatacg
     1801 tacggcagtt acgcgatcac ggtcttctgg tttttgttgt ttatcaacgc catcaatctg
     1861 atcgacgggc tggacgggct ggccggcggg ctgattgttt tcacctgcat tgtaatgaca
     1921 gtttcgactt tcatgcacca ggattatctt tccgcatttt attttgccgt gctgggcgga
     1981 tcggtgctgg gatttttgaa atataatttt aatccggcca ccatttttct gggcgacggc
     2041 gggagttatt ttctcggcta tacagtggcc attctggcca tccgcagttc cgcaaaatcg
     2101 catgtgggca ttttaatgtt gattcctctg ctggcgctcg gcattccgat tttcgattcc
     2161 attctgtcgc cgatccgccg ttttatgctc ggcaaaccga tcttccatcc ggataaaggg
     2221 catattcatc acatcatgat gaagatgggg ctttcatcgc aaagggttgt ctacatgatt
     2281 tacggcatca cgatggcgct gtgcatcctg gctattatga tcattatttt gcgcgatcaa
     2341 acgttcgaag ggcttgttct cggcggcctt cttttgggaa tgcttattct ggtgcgcaag
     2401 ctgggttatt tcgagtatct ggctttggac aagttccagg gctggtttca ggatgtcacg
     2461 gatgtggcgg gcttcagcca gaaccggcgc agctttcttg ccctgcagat tgcaacaagc
     2521 aagtccaaaa atatggatga attgtggcac aatattgtcg aagcgatgga gatgctgaaa
     2581 tttcatcacg caaaacttat ggtgatcgat gaacctgtcc gcgaatggac gatgccggaa
     2641 gacggcgaac atgcttactt ctgcgccccc tcttccgaag cggacctgga cgggatgctc
     2701 aggtttgaaa ttccgcttag agaatacggc tccgatacgt ttatgggcaa attgattttg
     2761 atcaaagatc tgaaaaaagg gtttttgaaa tcgtacacga tacgccgtgt tgaacatctg
     2821 cgccgatcat tgataccggt tttgaaaaaa ctgaaactca aggatgggtg atggnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgg tatgacggac agggcgtcat
     3001 cacccggctt agaataattg cctaacattt attacatcat taatataatg ttaggcattt
     3061 ttattattgt attattgcct aacatttagc atatattaat tattattgtt aggcattttt
     3121 tagtcgctat ttatagaaaa tttgactttt cggggccatc cgggcttatt tgcctaactt
     3181 atatttaatt taaatatatt tgttaggtaa atttttgttg actttttgcc taacagtttt
     3241 ataatagcgc atatttgtta ggcataatat tatatggaaa tcataaatcg gcttctggaa
     3301 gaagaactca aacggctggg tacgcttcat gatttttatg aaaagaaaat catggaatcg
     3361 ccccgcggat cgttatcggt aaaagccagg ggcaagaatc gatacctgta tctggcgcgc
     3421 cgcgaagata aaaaagtggt cttcgagtat gtcgggaagg atgtcgctta cgtgcggcat
     3481 gcgttaaatg aacagatgaa gcagcgccgg gagtatcagg cgaagcttcg cgaggtgaaa
     3541 gaaaatatca aggaggtaaa acggtcgctt cgcacaaaaa gaaaccggga cggtccggca
     3601 gccgtaaatt catcttaggg ggccgccggc aagaaaagcg tgcgggcaag aaatggaatg
     3661 tttaacaagg agggatggct tatgtttcag atcgtcaacg gtttttttaa tgatatctca
     3721 aacataaaca ttttaaattt aatcatcaaa aagggagaat ggattatgaa aaaatttatc
     3781 tttttaagca ttattttgtc gttgtttgcg gttttttcgg tggcacaggc ggaggtcagg
     3841 gacaggtcct tttcactcac gccgtatgtg ggcggttatt tctatgaggg caatcaggac
     3901 ttgaaagatt ccgccctgtt cggtttgcgc gccggttata attttaccag gcatttcggg
     3961 attgaagggt ttgccagtta tacccagacc gagatcgatg attcgtatta ttccggcaca
     4021 tccccctggc aaaacattta taatttcggc gtggaggcga tttatcattt cctgccggaa
     4081 aaacgttttg ttccgtttgt cgccatgggg ctgggcgggg ttcattatga agaagccaga
     4141 aattacaagt cgcattacgg tgctcccgac tacggcaaaa ttttcgaggc caccaaactg
     4201 gccgtcgact acggcgcggg catgaaatat tttgtgacgg ataatatcgc gctgcggctt
     4261 gatgtgcgcc atattctgcc cctgaatgga cgctggaata atccgcacta catccacaat
     4321 gaactacagg cgacgttcgg cgttaacttt gccttcggcg gaggacctaa ggttgcagtg
     4381 caggaagccg ccgttgaaga agcgcccgta cagccggtgg tcagcgctgc cgacggcgat
     4441 aaggacggcg ttcccgattt ccgggataag tgtcccaaga cgcctgcggg ccgcacggtt
     4501 gatgcagacg gctgcatgcc ggattcggat ggagacggca ttctcgatta cgaggatcac
     4561 tgcgccaata cgccggccgg cgtgaaggtt gacgctgacg gctgtccctc ggattccgat
     4621 tttgacggcg tttccgatta tcgggataag tgccccggca cgccccacgg ttcgcccgtt
     4681 gatgaggacg gctgtctttt caaaaagacc tcgatgttcc tgaaaatcga attcgattcc
     4741 ggcaaatcgg tgatcaagcc gaggtatcat caggaaatca agagggtggc agactttatg
     4801 aagaagaatt cgaaggcaac ggcaaccatc gtcggccata cggataacac cggcggccgc
     4861 ctggccaata tccggctgtc gcagaaccgc gccgacagcg tgcggcagta tctgatcagc
     4921 aagttcggca ttgccgccca acgcatcaaa gccatgggat atggaccgga cagaccgatt
     4981 gccggcaaca aaacagatgc aggacgcaag aaaaaccgca gagtggtcgc ttcttttgaa
     5041 tagatttttg ctgtagtttt ccgattctcc cgaatcagag ccttccggtg tcgttacgat
     5101 gccggaaggc tagctgtcat tagatttccc gtctctccgc tgttaataaa gttcaaggtt
     5161 caatgttcaa ggttcaaggt taaagaaaaa tagttttaag ttttaggttt taggtaaaaa
     5221 acacaaggct tcgcacatgc gttcgaaatg acatactctc gatgttgtga caaaggaatg
     5281 ttcgaaggga cacggttccc ctgtaattcc cgaatggata caacattctc atccctgagt
     5341 gggccacacc ttttgtcatc cccgagtggg tttgtcgggc catggtgccc tctgggtaga
     5401 tctagtattt attcttttct ggattccggc taggcccatg ccggaatgat aatgagccaa
     5461 gctgatagtg aggactcgtt gaaatcgagt gcaacgatga ttgaaacgtt agccgaacta
     5521 tcctaggagc gtagcgacgt agggaagcaa gcgaagcgct ggtcgaagcg taaggcgaat
     5581 tatccccgaa gcgtagcgga tggggacggt ggtgctggat tcccgccagg gacatgcg
//
LOCUS       DAOSME010000104         5619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_15798_length_5619_cov_22.333932, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000104 DAOSME010000000
VERSION     DAOSME010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15798_length_5619_cov_22.333932"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLG98_10635"
     CDS             complement(<1..197)
                     /locus_tag="PLG98_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33761.1"
                     /translation="MSKSDYHDFKPILAEIEERPTNPLGTYFLWTIISLMIIVLLTLF
                     LVKIDVVVSARGKIIPIGDVKV"
     gene            complement(194..2299)
                     /locus_tag="PLG98_10640"
     CDS             complement(194..2299)
                     /locus_tag="PLG98_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOE33762.1"
                     /translation="MAETGLVGIEVISKIHTVSIDMQAVKRRYFIETELSREEVLRIL
                     KDHGIRSRLKKLKSVDDLLKYPCPLIIISPENEFHILLGKKEDKIFIFDCNEKKPREM
                     TTDEFLKFWNGEAIAVYPRFTKTEFFLNMKWLFKEFYKHRPVFTAVITASFFIQLFGL
                     VTPLFIQVIIDKVLAHHALTTLQVVAGAFLVVLVFDTIMNLMRNYLLYHTANKVDAGL
                     GAKVYRHLLSLPFRYFETRKVGNIIARVRELENLRQFMTNISLTVLLDTVFSVVFIVI
                     MWVYSGYLTLIVLGIVFLIALISFIATPMIKQRLDEKFQKGAHNQSFLVESITGIQTV
                     KSLAIEGKMIKDWEKSLGEYIMSAFRLSNLGNVAVTSSQTLQKAMSLAVIYFGVSLVF
                     DKSMSVGQLIAFQMFASQLSGPILRLVRMWQDFQQARLSLDRIGDIINTPPEVVGGAI
                     TVKELKGEIVAKDVRFRYAPEALLVLDGVNFTIQAGMMVGIVGRSGSGKSTIAKLIQR
                     LYLPVEGTMMVDGVDIRHMDPLFLRYRTGVVLQECYLFSGTIKENIAMAAPNAGMERI
                     IQAARIAGAHDFISEMPMGYDTYVEERGSSLSGGQKQRIAIARALIMNPNILIFDEAT
                     SSLDYESEKIIQQNLNLIRQGRTVIFIAHRLSIMRDCDMIIVIDRGKIVETGNHEALM
                     QKDGLYAYLYKQQEENRLR"
     gene            complement(2271..3635)
                     /locus_tag="PLG98_10645"
     CDS             complement(2271..3635)
                     /locus_tag="PLG98_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE33763.1"
                     /translation="MLKFSSIVSRVFLLFICFTALPCRADSLDYRDVITDSIGYCASL
                     RVKSEDVFISDAQYRSNFAALYPVVTLGGRAERYENIDNRDNTTINTIDNEVVGGYDS
                     AWRSSVSLSGQYAVSNWYKKIYETGYYKKLKDTSIHECEAEVKKTIRDVTDIFRSVAE
                     SKIKLMYAREILSRLNEIFRMKQAAFTNGQFSHEDVLTAQTEVLNMEKDITGIHKDMR
                     ESLEKLSNYTGKSYSVDVDIISLPLSSSLPTPDETEIIAQTPEYKARLNEIEALRSRK
                     KSVSNNFLPDISVYGRYDFYNSSPDNMHDSLSDVRATDYSVGLHISLPLFDGGVRKWE
                     REKSFHELKKAQEYARLVFNEKNKDIKTLRVGYAELSKSYSHYKKINEQYEKIKDINR
                     KSKVLGERSDLDVIEFEKDALLAERDLKIIEHTMAVYEKQLALELNFNQFAGEYGGNG
                     TCRH"
     gene            4948..5364
                     /locus_tag="PLG98_10650"
     CDS             4948..5364
                     /locus_tag="PLG98_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33764.1"
                     /translation="MRETEDIISNLSFSHDGKNVVFDRCHEGDCQIQVYNLETGELAA
                     YQSPRNEQWTMGKYSYDGKKITFSVIPIKPNDDLDLGNMQIAVMDADGKNLKKVTTGS
                     GAKLYPTFSHNGKKILYACAARIREKGKTPAADYDA"
BASE COUNT         1577 a         1249 c         1248 g         1545 t
ORIGIN      
        1 accttgacat cccctatcgg gatgattttc ccgcgtgctg aaacgacaac gtcgatttta
       61 acaagaaata gagttagcag aacaataatc atcagcgata tgatagtcca cagaaaatag
      121 gtgcccagcg gatttgtcgg ccgttcttca atttccgcca gaatgggctt aaaatcgtga
      181 taatcggatt tgctcatctc agtctgttct cctcctgttg tttatagagg taagcgtaaa
      241 ggccgtcttt ttgcatcaga gcttcatgat tgccggtttc cacaattttg ccccgatcaa
      301 tcactataat catatcgcaa tcgcgcatga tcgaaagcct gtgcgcgata aatataacgg
      361 ttctcccttg cctgatcagg ttcaggttct gttggatgat cttttccgat tcataatcca
      421 gagagctggt tgcttcgtcg aaaattaaaa tattgggatt cataatcagc gcgcgggcaa
      481 tggcgattcg ctgcttttgg ccgccggaca atgaagagcc tctttcctcg acataagtat
      541 cgtaccccat gggcatttcg gaaataaaat catgagcgcc ggcaatccgc gccgcctgga
      601 tgattctttc catgccggcg ttgggcgccg ccatggcaat attttcttta atggtgccgc
      661 tgaaaagata acattcctgc aaaacaacgc cggtgcgata gcgcagaaac agcgggtcca
      721 tgtgacggat atccacgccg tcgaccatca tggtgccttc gaccggcaga taaagccgct
      781 gaatcagttt ggcaatcgtg cttttgccgg aaccgcttcg tccgacaata cccaccatca
      841 tacctgcttg gatagtgaaa ttgacaccgt ccagcaccaa cagtgcctcc ggcgcatagc
      901 ggaaacggac atccttggca acaatttcac ctttcagttc tttgaccgta atggcgccgc
      961 cgactacttc cggcggtgta ttaatgatat cgccgattct gtccagtgaa agcctggcct
     1021 gttgaaagtc ctgccacata cgcaccaggc gcaggatcgg tccgctcagt tgcgatgcga
     1081 acatctgaaa agcgattagt tgccctacgc tcatgctttt atcgaaaaca aggctgacac
     1141 cgaaataaat aacggcaagc gacatggcct tttgcagcgt ctgcgaagac gttaccgcca
     1201 cattgcccag gttggacagc ctgaacgcgg acataatgta ttcgccgagg cttttttccc
     1261 agtccttaat catcttgcct tcaatagcca gcgattttac ggtttgaata ccggtaatgg
     1321 attctaccag gaaagattga ttatgcgcgc ctttctggaa tttttcatct aatcgttgct
     1381 tgatcatcgg tgtggctata aatgaaataa gcgcgataag aaaaacaatg cccagcacaa
     1441 tgagtgtcaa atatccactg taaacccaca tgatgacgat aaacaccacc gaaaacaccg
     1501 tatcgagcaa aacggtcaaa gaaatattgg tcatgaactg ccgcaggttt tccagttcgc
     1561 ggacacgggc gatgatattg cctactttcc gggtttcaaa ataacgaaac ggcagcgaaa
     1621 gcagatggcg gtaaaccttt gccccgagtc ctgcgtcaac cttgttggcg gtatgataaa
     1681 ggaggtaatt gcgcatcagg ttcatgatgg tatcgaaaac cagcacaacc agaaaagctc
     1741 ccgcgacgac ttgtagcgta gttaacgcgt gatgggcaag gaccttgtca ataatgacct
     1801 ggataaataa cggcgtgacc aggccgaaca actgaatgaa aaaagaagcc gtaatgacgg
     1861 cggtaaaaac cggacggtgt ttatagaatt ctttaaagag ccatttcatg ttcagaaaaa
     1921 attccgtctt cgtaaaacgg ggatacaccg cgatggcctc gccgttccag aatttaagaa
     1981 attcatcggt tgtcatttcc cggggctttt tctcattgca gtcgaagatg aagatcttat
     2041 cttccttttt ccccaggaga atatggaatt cgttttcggg agagatgatg atcaagggac
     2101 acggatattt cagcaggtca tcaaccgatt tcagcttctt cagacgcgaa cgaatgccgt
     2161 gatccttcag gatacgcaaa acctcctccc ggctcagttc ggtttcgata aagtagcgcc
     2221 gtttaacggc ttgcatgtct atgcttaccg tgtggatctt ggagataact tcaatgccga
     2281 caagtcccgt ttccgccata ttcacctgca aattgattaa agttcaactc gagcgccagt
     2341 tgtttttcat aaacagccat tgtgtgctcg atgattttca gatcgcgttc cgccagcaga
     2401 gcgtcttttt caaattcaat aacgtcaaga tcgcttcttt cgccgagaac ttttgatttt
     2461 ctgttgatgt ccttaatttt ctcatattgt tcgttgattt ttttataatg cgagtatgat
     2521 ttcgaaagtt cggcgtatcc gacccggagc gttttgatgt ccttattttt ttcgttgaaa
     2581 acgaggcggg cgtattcctg cgccttcttc agttcatgaa aagatttttc cctctcccat
     2641 ttacggacgc ctccgtcaaa caagggcagg ctgatgtgaa gtccgacgct gtaatcggtc
     2701 gcacggacat cactgagcga atcatgcata ttatcggggc tgctgttgta aaaatcgtat
     2761 cggccgtaaa cggaaatatc cggcagaaaa ttattgctca ccgatttttt cctggatctg
     2821 agggcctcga tttcgttcag ccgcgcttta tactcggggg tttgtgcaat aatctcagtt
     2881 tcgtccggtg tcggcaagga ggatgacagc ggcaaagaaa tgatatccac atcgacggaa
     2941 taagatttgc cggtatagtt gctcagtttc tcgaggctct cgcgcatgtc tttgtgaatg
     3001 ccggtaatat ccttttccat attcaaaact tctgtttgtg ccgtcagaac gtcctcatga
     3061 gaaaattgtc cgttggtgaa agctgcctgt ttcatcctga aaatttcgtt aagacgcgac
     3121 agaatttcac gggcgtacat cagcttgatt ttgctttctg cgacagagcg gaaaatatcc
     3181 gtaacatccc ggatcgtttt ttttacttcc gcttcacatt catggatgct cgtgtccttg
     3241 agttttttat aatagcccgt ttcataaatt ttcttgtacc agttggaaac ggcatactgt
     3301 cccgataaag aaacggacga tctccacgcg ctatcataac cgccgaccac ctcattgtct
     3361 atcgtgttaa tcgtcgtgtt gtcacgatta tcgatatttt catatctttc cgcgcgcccg
     3421 ccgagggtga cgaccggata gagagcggca aagttgcttc ggtattgcgc atcggaaata
     3481 aaaacatcct cactcttgac acgcagagac gcgcaataac cgatgctgtc ggtgatcaca
     3541 tcgcggtaat ccagagaatc agcccggcag ggaagagctg taaagcaaat gaacaataaa
     3601 aaaacgcgtg aaacaatgct gctgaacttt aacaattatt cacactgtct aactatgttt
     3661 atttttgctg ctgtaaatgg aaaaatattg cctcgactgc gacaatcgcg ctgcatatat
     3721 caatttgcaa tcaaggatgc aaataaaatt tgctaaacaa ataggcctct gtccctgatc
     3781 aatatttatc cggttatcat caggaaatgc gttgagggaa ttccggcgcg gttgccggga
     3841 aattcttcgg cttttcggca gtttcgaaca atctgaaaat agggcactga cagttgtttg
     3901 ccgaaataaa atcccgtttc tatatgtaaa aaagtcaaga ctaatttaca caatcatgta
     3961 aaaaatcgaa acacttcatt ttgtcatttc ccacgatgat cgggcgaagt gcgactcaca
     4021 gatcatatag ggtaaattca gaagcctttt ctcgccactt ttttttctat cattttcatt
     4081 tcggctaaag ataacatcat tcttgataaa acaaatatca tttttaatga attttattga
     4141 caagaagata atttttattt attaaattcg cgtcttatag aatcttatag aatcaaaaag
     4201 cttcacaaga aaagtttcac tgattcacgg ttgttcaaat caaaaggatg aactgcttta
     4261 aacggttcat gctaaggaaa aagagtgaaa gttccttttg atcaacagaa ttgttatgtg
     4321 attcgctatg ctgatcgttt tcttacctca gtttatcatt cggaatacca tggtaatcca
     4381 agaagcattt ctaatattat tccgcgaata attaatcata ataaaacatt tgttttggaa
     4441 cgtaactgtg tcgctacagg actgcgagta tctttatgcg aaaaatgaag gaggaattaa
     4501 tgagaaaaaa taaggaactt atttcaaaaa atagaaccgt cccattatgg acatatttaa
     4561 tgagaaaaca taccggagct tcgctatcag aaattgggaa tcggttcgta atgcgcagtt
     4621 acagcagcgt gagcagtgtt attcaaagga tggaacaggc attaagagag gatgtaggtt
     4681 tgcggaaaag aatagcggac gtggaggcaa tattgccatg agccaagagg agacttgacc
     4741 ccttggactg cctggaaggt cgcgtgtttc attgcgcgaa aaattaagga gggcggaatg
     4801 aaaaaaaggt acaacgaaaa catcaataga ttcattttca atatctctaa atctaatttt
     4861 cagcagataa tcagcagagt aattttatac tgtataacaa cattcatttt tataaacatg
     4921 atgacatcca taggaggttg ttttgccatg cgagaaacag aagatattat cagtaattta
     4981 agcttttccc atgacgggaa gaatgttgtt tttgaccgct gccatgaagg cgattgccag
     5041 atacaagtct ataatctgga aacgggagaa ctggcggcgt accaatcacc ccgaaatgaa
     5101 caatggacga tgggaaaata ctcctacgac ggcaagaaaa taactttttc tgtcattccc
     5161 atcaagccca atgacgacct tgatctgggc aatatgcaga tcgccgtcat ggatgccgac
     5221 gggaagaact taaagaaagt aaccaccggt tccggcgcca agctctatcc caccttttcc
     5281 cataacggaa agaaaatact ctatgcctgc gcggcccgta tcagggagaa aggcaaaaca
     5341 cccgccgccg actacgatgc ctgagaagtc ggtctgacca ccggcaaaca gacgcaactc
     5401 accttttttg aatattttta catgggttat ctgacctatt tccccgatga tgaacgattt
     5461 atttattatg gagatcgtcc gagcgtattt cagggcttag cgtcgtttgg taacgagacg
     5521 gccttcggga acaaaatgat tgagttggaa cgtcagggta gggggtcaag ggggtcaagt
     5581 ctttgcttgg ctcttgttcg ttcagaaaga atgcttatg
//
LOCUS       DAOSME010000105         5517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_16165_length_5517_cov_21.006957, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000105 DAOSME010000000
VERSION     DAOSME010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16165_length_5517_cov_21.006957"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..763)
                     /locus_tag="PLG98_10655"
     CDS             complement(89..763)
                     /locus_tag="PLG98_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE33765.1"
                     /translation="MALIEMKSITKDYHLGETVVHALRGIDLQIDKKEFVAVWGPSGS
                     GKTTLLNLIGAIDDPTSGELAIAGKNVQKLTDDQKSEHRNETIGFVFQSFNLVPVLSA
                     LENVMLPMQIRGASVAEAKKQALMRLEEVGLKDLIHNRPAKMSGGQQQRVSIARALVN
                     NPSLVIADEPTANLDSETAHMVIDIMRELNERDHITFIFSTHDQRLLDKVKRLVRLED
                     GKIIQE"
     gene            complement(996..1904)
                     /locus_tag="PLG98_10660"
     CDS             complement(996..1904)
                     /locus_tag="PLG98_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOE33766.1"
                     /translation="MASKFSKNYFVVILCAVFLLFSFNDVFAKSSKKSSHHRKHSTSQ
                     SVQKKKGGLVLRSAAVIVKDQQTGKLLIQKQAKAVMPIASITKLMTAMVVLDAGLDPD
                     ETITIEEEDATAVRGTSFRLAVGTDLTREEALLLALMSSDNRCAHALGRTYPGGYWNC
                     VRAMNAKAAALGLAHTRFRDTVGLSSNNVSSAKDLARLVDTAYQYEMIRDFTTRKKAT
                     IFTGWQMLKFRNTNQLVRDGCGWKIGLSKTGFTNEAGRCLVMQAKVAKRPLLIVLLDC
                     RNNKARINDANRIKSWLESKSHSQHT"
     gene            complement(2083..2922)
                     /locus_tag="PLG98_10665"
     CDS             complement(2083..2922)
                     /locus_tag="PLG98_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE33767.1"
                     /translation="MRVPENWASVIRLASFTIADEARQKSFIAILIICALFILMVRGC
                     YHGDYMMNGQVVDSAKIIIMVSKITFHIIAGGVLFLAALLSMRIFRRDRGDGTQSCIL
                     SKPVSRRQYVSGKILGLWVLLTAVMIVLHGIVFLITSVSANMVMPEFFSASIWCSLNV
                     LFVVLTVLLLSMMMPDIAALLCVIGIGMIGVAADGVFAVRHSPIGQAMMQHDAPSDFS
                     VWQIIYTVWPKLFGVQSFAASRIGAEGFGGPVFAVPILNVGIYCMIIGALLFWRFGKE
                     DID"
     gene            complement(2924..3631)
                     /locus_tag="PLG98_10670"
     CDS             complement(2924..3631)
                     /locus_tag="PLG98_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE33768.1"
                     /translation="MIELDAVTKKYGSLVAVDNVSFSVGAGECFALLGPNGAGKTTIV
                     KLLLDFTRPTAGHLSLNGLSTTNAKSRAFVGYLPENHRIPTHLSGWQYLKRCAALLDI
                     KGADADKQCQWAVTLTGMQGREHDKSGTYSKGMIQRFGLGAALIGRPKLLILDEPAAG
                     LDPIGIRDIRRLLESLKKDGMTVFLNSHLLSEVEKICDNAAVIRRGRLMVKDKISSLV
                     RDGDTLEDVFIRFVKEG"
     gene            3779..5314
                     /locus_tag="PLG98_10675"
     CDS             3779..5314
                     /locus_tag="PLG98_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOE33769.1"
                     /translation="MKTFPTLIRILLFFFLTASSCFGAEQVQFRILHLNDFHGFAEPQ
                     RLSGSAQTAGGLSFLAGSINQFRKHQPTLLLAAGDFIQGHPWTNLFEGKSTIEAMNVM
                     GFSAMVLGNHEFDFGQDVLKKRIAEAAFPVLAANVRGFPSVKPYVIKDISGLKVGIIG
                     LITEETSTTTHPKNVEGLIFSPVIDTAQQLVRELRNKTDLIIILSHLGLPADVALAGA
                     VEGIDIIVGGHSHTRVEKPMIVRNTLIVQAWEHGKVLGVLDVTVRNKKISGHEGRLIV
                     IDPVQNKPDPPVAQIVDRYQTQAAGILNHVIGEARVDLPGKESRCRETSLGNLVADIL
                     RQQTGADIALINGGGIRADIWKGPVRMNDILSVLPFSNRAIVLKVSGQEIKDIFEHGI
                     SDPEGKAGRYPQVSGMQLTYNPANPAGQRITGFTINGKPLQTDQRYTLATSDFLAAGG
                     DGYAAFTRILKTMNDGGQNTASENRALFFASGQDIRDMVIDFIRDNKMISAAVENRIR
                     KED"
     gene            5318..5512
                     /locus_tag="PLG98_10680"
     CDS             5318..5512
                     /locus_tag="PLG98_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33770.1"
                     /translation="MRVWLNNEPVDVVPGMKVREALIQAGFLPEVEKGGIVLDEWGNE
                     VGLDGALQDGSRYAFKKKAD"
BASE COUNT         1424 a         1404 c         1344 g         1345 t
ORIGIN      
        1 caatggcaat catcgtcaca acagagcggc gtttgtttct taaaatattc cggtaggcca
       61 gcttaatcca ctgcagcatc agacgttatc actcctgaat aattttgccg tcttccagac
      121 gtaccagccg tttgacttta tccagaagcc tctggtcgtg tgtggagaaa atgaacgtga
      181 tgtggtctct ttcattcagt tcacgcataa tatcgattac catatgggcc gtttccgaat
      241 cgagattggc cgtcggttca tcggcaataa ccagtgaggg attattgacc agcgcccggg
      301 caattgaaac tctctgctgc tgaccgcccg acattttcgc cggacgatta tgaatgagat
      361 ccttcaggcc gacctcttcc agacgcatca gcgcctgttt tttggcttcc gccaccgacg
      421 cgcctctgat ctgcatgggc agcatgacat tttccagtgc ggagagaaca ggcaccagat
      481 taaagctctg aaaaacaaaa ccgatggtct cattccgatg ctcgcttttc tgatcgtccg
      541 tcaacttctg gacatttttc cctgcaatgg ccaattctcc ggatgtggga tcatcaatgg
      601 caccgataag attcaggagg gttgttttgc ccgatccgga cggtccccaa acggccacaa
      661 attctttctt gtcgatctgg agatcaatgc ctctcagcgc atggacaacc gtctcgccaa
      721 gatgatagtc cttggtgata ctcttcattt caattaaagc cattttatac cccctggaaa
      781 aactcaacgg taaaccgtca tggattgcgg ccgacacttt gcaaagcaat catttttttg
      841 gcttgcggag catataaaga aatgtcctat atgtcaatta aatatcaaaa aaaaatagat
      901 aaaaaaataa aggcggtggt taggatgtta ttaattttca attgaaaacc gcttcacacc
      961 ggctctcaat ttcaaccgat acgatatacc cattattatg tatgttgaga atgtgatttt
     1021 gattccaacc aggattttat ccggttggca tcgttaatgc gtgccttatt gtttcggcaa
     1081 tcaagcagaa cgatcagcag cggacgtttc gccactttgg cctgcatgac cagacacctt
     1141 ccggcttcat tggtaaatcc tgttttggac agaccgattt tccagccgca accgtcccgg
     1201 acaagctggt tggtgttgcg gaatttcagc atttgccagc cggtaaagat cgtggccttt
     1261 ttacgggtcg taaaatcacg gatcatctca tactgatagg cggtatcaac cagtcgcgcc
     1321 aggtcttttg cggaagaaac gttattgctc gacaggccaa ccgtatctct gaaacgcgta
     1381 tgggctaatc ccaacgcagc ggcttttgcg ttcatcgccc ggacacagtt ccagtaaccg
     1441 cccggatacg ttctccccag tgcatgggcg caacggttat ccgaagacat cagcgccagc
     1501 aacaacgctt cctcgcgggt gaggtcggtg cccacggcaa gccgaaagct tgtcccgcga
     1561 accgctgtcg cgtcttcctc ctcaatggtg atcgtttcat caggatccag tccggcgtca
     1621 agaaccacca tggcggtcat cagtttggtg atagaggcaa tgggcatgac ggctttggcc
     1681 tgtttctgaa tcaacaactt accggtttgc tgatccttca caatcactgc cgccgaacgc
     1741 aaaaccagcc cgcccttttt cttctggacg gattgtgatg tggaatgttt tcgatgatgg
     1801 gacgattttt tggaggattt tgcaaatacg tcgttaaagg aaaataataa aaaaactgca
     1861 cataaaatga ctacaaaata atttttactg aatttacttg ccatgcattc tcccgatctt
     1921 caagatacgt ctttagtttt gcaacaaaga cctgagtgtc aatttaatgc cggcatttgc
     1981 tctgccaact tgtgtttcaa agttaatttt ttggaatcat acagaatttt ttcgaattag
     2041 tcaattttga atttgatttt tttgcaaaaa attccaggga tgtcaatcaa tgtcttcttt
     2101 tccgaaacgc cagaacagca gtgcgccgat aatcatgcag tagatgccga cattcaggat
     2161 cgggacggca aaaaccggtc cgccgaatcc ttcggcgccg atccgggaag cggcaaagga
     2221 ctgcacgccg aaaagtttcg gccacaccgt ataaattatt tgccaaacgg aaaagtcaga
     2281 tggcgcatcg tgctgcatca tcgcctgtcc gatcggacta tggcgaaccg caaagacgcc
     2341 gtcggctgca acaccgatca tcccgatgcc gataacgcaa agcaacgcgg caatatcagg
     2401 catcatcatc gacaaaagaa gcacggtcag caccacgaaa agcacattca gcgagcacca
     2461 gatcgacgcc gaaaaaaatt ccggcatgac catgttggcg ctgacggatg tgatcaaaaa
     2521 aacgatgcca tgcagaacaa tcatgacggc cgtcagcaaa acccacaggc cgagaatttt
     2581 tccggagaca tactgccggc ggctgacggg ttttgaaagg atgcaggatt gcgtgccgtc
     2641 accgcggtcg cggcggaaaa tgcgcatgga cagcagagcc gccagaaaca aaacgcctcc
     2701 ggcaatgata tggaatgtga ttttcgaaac catgatgatg atttttgctg aatcgacgac
     2761 ctgtccgttc atcatgtaat cgccgtgata acaaccgcgc accatcaaga taaacaaagc
     2821 gcagatgatc agtatcgcga taaaactttt ttgacgggct tcgtccgcaa tggtgaatga
     2881 ggccaggcgg atgacggaag cccagttttc cggaactctc atgtcaacct tccttcacaa
     2941 aacggataaa aacatcttcc agggtatcgc cgtcccggac gagggaagat attttatcct
     3001 tgaccatcag ccttccgcga cggatcactg cggcgttatc gcatattttt tccacttccg
     3061 acaaaagatg ggaattcaga aaaaccgtca taccgtcctt tttcagcgat tccagtaacc
     3121 ggcgtatgtc ccggataccg atgggatcaa gtcctgcggc aggttcatcc aggatcagca
     3181 gtttcggccg gccgatcaga gcggcgccca gaccaaatcg ctgaatcata cccttggaat
     3241 aggttccgga tttgtcatgt tcccttccct gcatgccggt cagtgtgacc gcccactggc
     3301 actgcttatc cgcatcggca cctttgatat cgagcagtgc cgcgcagcgc ttcagatact
     3361 gccacccgga aagatgcgtc gggatacggt gattttccgg cagatagccg acaaaagcgc
     3421 ggctcttcgc gtttgttgtg gaaagaccgt tcaacgacaa atgtcctgca gtcgggcggg
     3481 taaaatcaag cagcagttta acgattgtcg ttttccccgc tccgttcggt ccgagaagag
     3541 caaaacactc gcctgcgccg acggaaaatg aaacattatc cacggcaacc agggagccat
     3601 acttttttgt cacggcgtcg agttcgatca tcacgaatga acatatggtc cgaaggacat
     3661 tctgtcaaca aaattatcat aaaaacgcct gccgctcttt ccgtcccgta aacagctctg
     3721 gaattcttcc ggccattggg ctatattaaa aaaagtttgg aaaagaagga atttttccat
     3781 gaagacattt ccgacattga tccgcattct tttgtttttc tttctgaccg cttcatcctg
     3841 tttcggtgcg gaacaggttc agtttcggat tctgcatctg aatgactttc atggttttgc
     3901 cgagccgcaa cgcctgtccg gatcggcgca gaccgccggc ggattatctt ttcttgccgg
     3961 atcaatcaat cagttcagaa aacatcagcc gacactgctt ctggctgccg gagactttat
     4021 tcaggggcat ccgtggacaa acctttttga aggcaaatcc accatcgagg cgatgaatgt
     4081 catggggttt tcggcaatgg tcctcggaaa tcacgaattc gatttcggac aggatgttct
     4141 caaaaaaagg attgccgaag cagcttttcc ggttctggcc gccaatgtcc gcggtttccc
     4201 gtccgtcaaa ccgtatgtga tcaaagacat ctccggcctg aaagtgggga tcatcggatt
     4261 gatcacggaa gaaacatcca ccacaacgca tccgaaaaat gttgaaggcc tgattttttc
     4321 tccggtcatt gataccgcgc aacaacttgt ccgcgagttg agaaataaga ccgatttgat
     4381 catcattctt tcccatcttg gcctgccggc cgatgtggcc ctggccgggg ccgttgaagg
     4441 aattgacatc attgtcggcg gacattcgca tacccgcgtt gaaaaaccga tgatcgttcg
     4501 taacacactg attgttcagg cgtgggaaca cggcaaagtg ctgggggttc tggatgtaac
     4561 ggtccgcaat aaaaaaatca gcggacatga aggacggctg atcgtcatcg atccggtcca
     4621 aaacaagccg gatccgccgg tggcacagat tgttgatcgc tatcagacac aggcggccgg
     4681 cattctcaac catgtgatcg gcgaagcccg ggtcgatctg ccggggaagg agtcccgctg
     4741 ccgggaaact tctctgggca atctggtcgc cgatatttta agacagcaaa ccggcgccga
     4801 tatcgctttg atcaacggcg gcggcatccg ggcggatatc tggaagggtc cggttcgtat
     4861 gaacgatatt ctttccgtcc tgcctttctc caatcgcgct attgtcctga aggtcagcgg
     4921 acaagaaatc aaagatatct tcgaacacgg catttccgat ccggaaggga aggcgggacg
     4981 ttatcctcag gtttccggaa tgcaactgac ctacaatccc gccaaccccg ccggtcagag
     5041 aattaccggc tttacgatca acgggaaacc gttgcagacc gaccaacggt acacactggc
     5101 gaccagtgat tttctggccg ccggaggcga cggctatgcc gcgttcaccc gaattcttaa
     5161 gaccatgaat gacgggggac aaaacaccgc atcggaaaat cgcgcgctgt tttttgcatc
     5221 cggacaggac attcgcgaca tggtcattga ttttatcaga gacaataaaa tgatttccgc
     5281 cgccgtggaa aacaggatca gaaaggagga ttgattgatg cgtgtctggt taaataatga
     5341 accggtcgat gtggttcccg gaatgaaagt tcgcgaagcc ctgattcaag caggctttct
     5401 gccggaagtg gaaaagggcg gaatcgttct tgacgagtgg ggcaacgaag tcggtctgga
     5461 cggggctctg caggacggaa gccgttatgc atttaaaaaa aaggcagatt aaaaaaa
//
LOCUS       DAOSME010000106         5434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_16470_length_5434_cov_19.391151, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000106 DAOSME010000000
VERSION     DAOSME010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16470_length_5434_cov_19.391151"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1039)
                     /locus_tag="PLG98_10685"
     CDS             complement(116..1039)
                     /locus_tag="PLG98_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE33771.1"
                     /translation="MKRNILFKLLILVSFIFLLPAAGLARDIAPIVSTDWLSANLKNP
                     KLIILDVRRVEDYREGHIPGALNAFYGSWAYMKDGLFGSLPEKDDIDDTISYMGIDFD
                     NWVVVMGCMDTPRLSYQSARVACTLQYAGIENVSLLDGGINKWIAEKKPLSKKVEPRK
                     AKKFQGKYTGEVFADKNYVKERLGKLIILDVREREFFSGEKKMDCVPMRGHIPGAFNM
                     PTSCAFNENKTFKTKEELNAIVEAAAGKDHLAAIVTYCDMGQCCPIWSYLMKQVLGYT
                     NVKLYIGSMQEWMQDPQAPVTDNGKPLRKGE"
     gene            complement(1187..1669)
                     /locus_tag="PLG98_10690"
     CDS             complement(1187..1669)
                     /locus_tag="PLG98_10690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOE33772.1"
                     /translation="MTKKAFQDYYPDDLSHCYGCGSLNQQGMKIKSYWDGEESVCIHE
                     PKPYYTALPGYVYGGLIASLIDCHSTGTAAAAKYRAENREMDTMPPFRFLTASLHVNY
                     LLPTPLAGPIEVRGRVKEIKGRKVVVESRLIAGGKVCATGEVVAVQAPEHMMQALLKK
                     "
     gene            complement(1725..2678)
                     /locus_tag="PLG98_10695"
     CDS             complement(1725..2678)
                     /locus_tag="PLG98_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE33773.1"
                     /translation="MAEAALKVVEKVEILTLQDNYIEMTAMDNNAVVTRAMPLKDGEI
                     KASILAEHGFSALVKTTVEGKTHTLLFDFGFSENGAAQNVKTLGADLKEVEAVALSHG
                     HSDHTGGMSKMAELIGRTNVPFFVHPAAFQSPRYLKFSEDFKINFPKLTRDMIQQAGL
                     SLIETEKPCPMLDGTILFLGEIPHATDFEKGWPLPHCQKDGKEIWDSIEDDTSVVMNL
                     KDKGLIILSGCAHAGIINTVKYAIEVTGIDKIHVVMGGFHLSGPLFEPIIDRTTEELQ
                     NLNPAYVIPTHCAGRKAIMEMEKKMPQQFILNMAGTKLTFA"
     gene            complement(2842..3180)
                     /locus_tag="PLG98_10700"
     CDS             complement(2842..3180)
                     /locus_tag="PLG98_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE33774.1"
                     /translation="MKIAIPLAEGKLTMHFGHCASFALIDVDPQTKNILKREDIAAPP
                     HEPGLLPPWLAERGVNMIIAGGMGQRAQQLFAQQSIQVVVGAPAGTPEKLITGYFAGT
                     LQVGENVCDH"
     gene            complement(3194..4492)
                     /locus_tag="PLG98_10705"
     CDS             complement(3194..4492)
                     /locus_tag="PLG98_10705"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOE33775.1"
                     /translation="MSELVNLEESYPPAKKHANNPHNMGPMDTFNGHAKITGPCGDTM
                     EFWILADENKIKIVSFITDSCDPSRACGSMTTCLAEKKTIDEAKAISQQDVLKAVEGL
                     PEDHDHCALLATNTLKAACEDYLSKQKQMEDTKVEDKKTCDSCGDSSCSAQKKGAGES
                     EQEYADRRRLQMRLCHINHKIVVLSGKGGVGKSTVAVNLAMALALNGLRVGLLDVDIH
                     GPSVPTMLGLENETLKGTEGELQPVEFGGLKVMSIGFLLQNPDHAVIWRGPMKMGVIK
                     QFMTDVNWGDLDYLIVDAPPGTGDEPLSLCQLIQPLDGAVIVTTPQKVAAIDVRKSIS
                     FCQQVHVPVLGVVENMSGFVCPKCGELTQILPEGGGRKIATDMNVPFLGAIPMDPGIA
                     QSGDSGKVFIYHSSETPTGKIMQDIIDQITNFDKTKKLNS"
     gene            complement(4512..5369)
                     /locus_tag="PLG98_10710"
     CDS             complement(4512..5369)
                     /locus_tag="PLG98_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33776.1"
                     /translation="MKELVIISGKGGTGKTSVTASFAVLADRPVVCDCDVDAADLHLV
                     LEPTIRERHEFKSGHEAVIRQNDCTGCGICQDLCRFGAISNTVDLFSIDSVSCEGCGV
                     CVRFCPEGAMDFPQRHCGEWFVSNTRCGPMVHARLGIAAENSGKLVSTVRREAKHLAE
                     EENHKMVIVDGPPGLGCPVIASLTGASQVLVVTEPTVSGEHDLERILSLTRHFQIPTA
                     ICVNKWDINEEMTQQIEDKARRANAHVAGRIRYDRAVTRAQIQKKAVVEIDAPSAGDI
                     RQLWKNLNF"
BASE COUNT         1233 a         1406 c         1308 g         1487 t
ORIGIN      
        1 cacctacaac taaatgtatt tctgtttaga attggtgcgc ggattaccaa tagtaacaga
       61 gtattcttga tgtatacggc agatgaaatt agtgcacatc tgcctgcatc catgtttact
      121 cacctttgcg gagaggtttt ccattgtctg tcacaggcgc ctggggatcc tgcatccatt
      181 cctgcatcga accgatatag agttttacat ttgtgtaacc taggacctgc ttcatcagat
      241 acgaccaaat cggacagcat tgtcccatat cgcagtaggt cacaatcgca gccaagtggt
      301 ctttcccggc ggccgcttcg acgatggcat tcagttcctc tttggtcttg aaggttttat
      361 tttcattgaa ggcgcaggag gtcggcatat taaacgcgcc ggggatgtgt cccctcatgg
      421 gaacgcaatc catctttttc tcgccagaaa agaattcgcg ttcacggacg tccaggatga
      481 tcagtttgcc cagtctttcc ttcacatagt ttttgtctgc aaatacttca cccgtgtatt
      541 tgccctggaa tttttttgcc ttgcgtggtt caactttttt ggacaaaggc tttttctccg
      601 ctatccattt attgatgccg ccgtcaagca gggaaacgtt ttcgatgccg gcatattgaa
      661 gcgtgcaggc gacgcgggcg ctttgatagc taaggcgcgg cgtatccatg cagcccataa
      721 ccaccaccca gttgtcaaaa tcaatgccca tgtagctgat cgtatcatca atatcatctt
      781 tttccggaag cgaaccgaac aagccgtctt tcatataggc ccaggaacca tagaaggcgt
      841 tcagtgcacc gggaatgtgt ccttcgcgat aatcttcgac gcggcggaca tcgagaataa
      901 tgagtttagg attttttagg ttggctgaaa gccagtcagt ggagacgatc ggggcgatat
      961 cacgagccag gcctgccgcg gggagtaaaa agatgaacga aacaagaata aggagtttga
     1021 acaaaatatt tcttttcata ttacaatctc catttcataa tttcttgctg ttatttaatt
     1081 catttcatca ttcctgcctg tatgaaacac agtatatcat accctaatgt aaaaagaacc
     1141 agccgatatt gatccagatc aaagctggga catttatttt taggagctat tttttcaata
     1201 aggcctgcat catatgttcc ggtgcctgga ccgcaacaac ctcgcctgtc gcgcagactt
     1261 tgccgccggc gatgaggcgg gattccacta ctaccttgcg tcccttgatt tccttaacgc
     1321 ggccgcgcac ttcaatcggc ccagccaaag gcgtgggaag tagatagttc acgtgcaacg
     1381 aggcagtcag aaatcggaac ggcggcatcg tatccatttc acgattttca gcgcgatatt
     1441 tggctgccgc agccgtgcct gtcgagtggc agtcgatgag cgacgcgata agcccgccat
     1501 aaacataacc gggcaacgct gtgtagtatg gtttcggttc gtggatgcaa acagattctt
     1561 caccgtccca atagcttttg attttcatac cctgttggtt gaggctaccg cagccatagc
     1621 aatggcttag atcatccgga tagtagtcct gaaacgcctt ttttgtcatg taatgtcctt
     1681 gtttgttatg agttaaaaaa atcatccttt acgcttcacg agttttacgc aaaagtcagt
     1741 tttgttcccg ccatatttaa aataaattgc tgcggcatct ttttttccat ttccataatc
     1801 gcttttcgtc ctgcgcagtg cgtcggaatc acatacgccg gattcagatt ctgaagttcc
     1861 tcggtcgtgc ggtcgataat cggttcaaaa agcgggccgg agagatggaa tccgcccatg
     1921 actacgtgaa ttttgtctat ccccgtgact tcaatggcat atttgaccgt attgatgatt
     1981 cccgcatgcg cgcatcccga aaggatgatc agtcctttgt cctttaagtt catgacaaca
     2041 gacgtgtcgt cttcgatgga atcccatatt tccttgccgt ctttctggca atgagggagg
     2101 ggccaccctt tttcaaaatc cgtggcgtgt ggaatttcgc ccagaaaaag aatggtgcca
     2161 tcaagcatcg ggcagggctt ttccgtttcg attaaggaaa gtcccgcttg ttgtatcatg
     2221 tcacgcgtca atttcggaaa attgattttg aagtcttcgc tgaatttgag gtagcgcgga
     2281 gactggaagg ctgctggatg cacgaagaaa ggaacgtttg tgcgccctat taactctgcc
     2341 atcttactca tgccacctgt gtgatcactg tgcccgtgcg aaagggccac agcctcgacc
     2401 tccttcagat cagcacctag cgttttgacg ttctgcgccg cgccgttttc ggaaaagccg
     2461 aaatcgaaaa gcagtgtgtg cgttttacct tccacagtgg ttttaaccag cgctgaaaaa
     2521 ccatgttccg ccagaatgga ggctttgatt tcgccatctt ttaaaggcat ggccctggtg
     2581 acaacagcgt tgttgtccat ggctgtcatc tcaatgtagt tgtcctgcag ggttagaatc
     2641 tcaacttttt ctacaacttt taatgctgct tccgccatta ctttactcct ccttttttag
     2701 ggctagccga aaaacgacta tttcaattcc atgaaaatat tccggcaaga tgtcagaatt
     2761 atacatcatg aacttatttc gtcaaacggt agcacagatt ttaaaacgtt ttggttcaat
     2821 cgtaagcgcc atgtgtccta ttcaatgatc gcagacgttt tctccaactt gcagcgtgcc
     2881 cgcaaagtag cctgtgatca gcttctcagg cgttccggcg ggtgcgccga cgaccacctg
     2941 aatgctctgt tgggcaaaca attgttgggc tcgctggccc atcccacccg caatgatcat
     3001 gttcacgcca cgttcggcga gccatggcgg caagagcccc ggttcatgag gcggcgcggc
     3061 gatgtcctcg cgcttcagaa tgtttttggt ttgcgggtcc acgtcgatca aggcgaaact
     3121 cgcacagtgc ccaaaatgca tggtcagctt tccttcggct aatggtattg caattttcat
     3181 ggtatttttc tccttatgaa tttagttttt ttgttttatc gaaattcgta atctgatcaa
     3241 tgatgtcctg catgattttt cccgtcggtg tttcggacga gtgataaatg aacactttgc
     3301 cgctatcgcc ggattgtgca atgcccggat ccatcggaat cgcgcccaga aacggcacgt
     3361 tcatatcggt ggcgattttg cggcctccgc cttccggcag gatttgggtc agctcgccgc
     3421 atttggggca gacaaagccg ctcatatttt ccacaacccc cagcaccggc acatggacct
     3481 gctgacagaa gctgatggat ttgcgcacat caatggcagc caccttctgc ggcgttgtga
     3541 caatcaccgc gccgtcgagc ggctgaatca actggcaaag cgagagcggc tcatcgcccg
     3601 tgccgggagg ggcatccacg atgaggtaat ccagatcacc ccagttaacg tccgtcataa
     3661 actgcttgat gacgcccatc ttcattgggc cgcgccagat caccgcgtga tcggggtttt
     3721 gcagcagaaa gccgatggac atcactttca gaccgccaaa ttccactggc tggagctctc
     3781 cttcagtgcc cttgagggtt tcgttttcca gacccagcat ggtgggcacg ctcgggccgt
     3841 ggatatcgac atccagaagt cccacgcgca gaccattgag agccagcgcc atggccaggt
     3901 tgaccgccac tgtgcttttg ccgacacccc ctttgccgga gagtaccacg attttgtgat
     3961 tgatgtggca aagcctcatt tggagacggc ggcggtcggc atattcttgc tcgctttcac
     4021 ccgccccctt tttttgggcg gagcagcttg aatctccgca cgaatcacat gttttcttat
     4081 cttccacttt tgtatcctcc atttgttttt gtttagaaag gtaatcttca caagccgcct
     4141 ttaaggtatt tgtcgccaga agcgcgcagt ggtcgtgatc ttcgggaaga ccttccactg
     4201 ctttgagaac atcctgttga cttattgctt tggcttcatc aattgtcttt ttctcggcca
     4261 gacaggttgt catactgccg caagcccgcg aaggatcaca gctatcggtg ataaaagaga
     4321 ctatcttgat tttgttttcg tcggctagaa tccagaactc catggtatcg ccgcagggac
     4381 cggtgatttt tgcgtggccg ttaaaagtat ccatcggccc catattatgg gggttgttgg
     4441 cgtgtttctt tgccggagga taagattctt ccagatttac taattcgctc atttttactt
     4501 ttttatacct cctaaaaatt aagatttttc cagagttgtc gaatatcgcc ggcgctgggc
     4561 gcgtcgattt caacaacggc tttcttttgt atttgcgccc gggtgacagc tcggtcgtag
     4621 cgaattcgcc cggctacgtg cgcattggcg cgtcttgctt tatcctcgat ttgttgtgtc
     4681 atttcctcgt taatatccca cttgttgaca caaatggccg taggaatttg aaagtggcgt
     4741 gtcaaagaca atatcctttc cagatcatgc tcgccggaaa ccgttggttc cgtaacgacc
     4801 agaacctgtg aagcaccggt gagtgaggcg atgaccggac agcctagccc aggcgggcca
     4861 tccacaataa ccattttatg gttttcttcc tcggccaggt gtttggcttc ccgacgcacg
     4921 gtggaaacca gcttgccgga attttccgcg gcaatgccca ggcgcgcatg caccatcgga
     4981 ccgcagcgcg tgtttgaaac aaaccattcg ccgcaatgcc gctggggaaa atccatggcg
     5041 ccctccggac aaaaccggac gcaaacgccg caaccttcgc aggaaaccga atcgatggaa
     5101 aacaaatcca cagtattgga aatcgcgccg aaacggcaca ggtcctggca gatgccgcaa
     5161 ccggtgcagt cattctggcg gatcacggct tcatgcccgc ttttaaattc atggcgctcg
     5221 cggatagtcg gctctaaaac cagatgcaga tccgcggcgt ccacgtcgca atcgcacacg
     5281 acaggccggt cggccaacac ggcgaaagaa gccgtgacgc tggttttgcc cgtccccccc
     5341 ttgccgctga tgatcactaa ttctttcatt gcaaaccttt tttataaaat gatacgtctg
     5401 gccaccagca catcctggta ggtgtcgtga tact
//
LOCUS       DAOSME010000107         5430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_16483_length_5430_cov_19.474791, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000107 DAOSME010000000
VERSION     DAOSME010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16483_length_5430_cov_19.474791"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLG98_10715"
     CDS             <1..648
                     /locus_tag="PLG98_10715"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HOE33777.1"
                     /translation="PERIRNLICAISKAGFFDRSALIGSWLMPIYQELYGTRYVLRTM
                     DIDFAVHIAHTKIAPRADLNQCITDLGFVDYFTADGMQRFTGGGYEVEFIGQRAGGRD
                     KGFLSVPEWHLTAMPLPFINILIDYSTTTMLCDFSIRFPVPEAFFLHKLITAQKRLSD
                     NKRMKDIEQCSVIAKIINDDRLQQIIHNQRFSKATKKYIALSCAAIEFPLHRIGM"
     gene            824..3049
                     /locus_tag="PLG98_10720"
     CDS             824..3049
                     /locus_tag="PLG98_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE33778.1"
                     /translation="MFKKRRRHVHHTQPVHGNLDKNERFIKRQCCIYLERLLAVFPLT
                     DRDTLEMLTYVLGPKISELKKTISKKVYSKQRHTPVFDTDDELDILDDDSDDFYQMIK
                     HAGKKIQKEFITLSRTVLAKRVKTLSYAGKSEMEKKLDTLDKMFALSNEEKAFTTLLY
                     INTVWPRADEYFVDHLHCQDIFGRRYLKVMLQITDSGINRILSGTLARIEFFEFSNHS
                     FNLSDDYLGFFQKPLKDVLKKKDFVRFSGKTIPLKNHLIDPGVTKHILSLLRTKRESS
                     NHILLYGPPGTGKTSYALGLVNKLKIPAYAIMDDSCNQTRTRRAAIIACRNMTNDGEG
                     SLIIVDEADSLLNTEESWSSGGEIKDKGWLNQLLEEPGARMIWITNSIEDMEDSVLRR
                     FAFSVHFPAFNRRQRFSLWESVLCRHHVRKLFKDDEINKLTARYPVSAAIINMAVTKA
                     LESAAADHIAFKEIMIMNLDAHWKLLNGGAKPKTGDRIEEHYSIEGLNIEGNLPVILA
                     QLDSFDRCLRAPDKSGVRNFNLLFYGPPGAGKSELARYVAKRLDRELMVKRASDIVSP
                     YVGETEQNISRVFSEAESMEAVLVIDEADSFLFSRDRAVRSWEISHTNEFLTQMERFR
                     GILICTTNRFADLDMASVRRFNQKIGFGCLKPDGNMIFYQKLLCELCKTPVNRNQEEL
                     IGRIHDLTPGDFRIVRDRFAISDEKCVTHEQMIQALEAEARVKKTQQGNRPIGFMRRT
                     A"
     gene            3111..3863
                     /locus_tag="PLG98_10725"
     CDS             3111..3863
                     /locus_tag="PLG98_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33779.1"
                     /translation="MEEGKHFIGVGDTGHRAAKELCRIYEEMRGCLFESEQDRPHNHL
                     RIHSYPCAETACFDDKDHLVILVGSLIDPGLQDARTSFHESRPYFMLTVGIDHQTRVD
                     ADDIQPLPDECLIYPDTSLSVPVKLAQLVLQIFLIHTPWYISKRGALIGYDLYDTKQL
                     FGGKVVKAISMTSDRAHYRQNFSMFLDKNRADIRRTKGILMSVWGLSIMQANEILKET
                     RLLLTAGADELFTCHNLPADEPNILVTLFLAF"
     gene            4211..5428
                     /locus_tag="PLG98_10730"
     CDS             4211..5428
                     /locus_tag="PLG98_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33780.1"
                     /translation="MKVTGGINWLVMGVLKNKEKFCAVLDLEMRPLFRKCGLANLVKH
                     TELELARREKCDFIQTWHWAANPNFNAAIAPGLKSGFILYHGQPQDGDVYEDRGHVHL
                     RYYFDETKKRSVEVKTKDGKVFMSPIDNHAIIDYLEACPNKYPGRMIQSIEEYSKGKA
                     MTPIKKNKRIAEKHNGGSERQRIFIAQGAAGFEYTRQRNTYRIGDSISFYPILQIDHF
                     KKSSQNAPYLKHVVYNIYEFCFKVDAVQRLEETHGMMRNDYLMELLPNFKVCCRDWNK
                     ENQFIVDKFYEGYGRLTIGDHRSSMHYKQPPHQQNILMKGKLVGISDDLMQRDFSTKY
                     AHQVKRFRYGKDVYGDEDALEWFGYYRDLKKFKKNVISYTTNPVEYESTDSFALYGYS
                     PKLIFCIEIIAPQ"
BASE COUNT         1554 a         1175 c         1308 g         1393 t
ORIGIN      
        1 cccgaaagga tcaggaatct tatttgcgcg ataagcaaag ccggattctt tgatcggtct
       61 gctttaatcg gaagctggtt aatgccgata tatcaggaac tctatggcac caggtatgtt
      121 ttgcgaacca tggacattga ttttgctgtg cacattgctc atacaaaaat tgcaccgcgg
      181 gccgatctca atcaatgcat aaccgattta ggatttgtgg actatttcac cgctgacgga
      241 atgcaaagat ttaccggcgg cggttacgag gtggaattta tcggacaaag agccggaggc
      301 cgagacaaag gcttcctttc tgttccggaa tggcacctga cggccatgcc gcttccattc
      361 atcaatattc tcattgatta ttcgacaacg acgatgcttt gtgacttttc aattcgcttc
      421 cctgtgcccg aagcattctt tcttcataag cttatcacgg cccagaaacg tttatccgac
      481 aacaaacgaa tgaaggatat tgaacaatgt tcagtcattg caaagattat caatgatgac
      541 cgattgcaac agatcatcca caaccagcgc ttcagcaaag cgactaaaaa atacattgca
      601 ctatcatgcg ccgctatcga atttccgcta catcgaattg gaatgtaacc ggcagtagtg
      661 gaaattatca agtcatatca aatctctatc ctatcttctt ttttccaata aataatcaac
      721 attcgtgccg ccaaatggaa cgatccggtg ttatgattta tccaaaagca cgagcctcct
      781 ctattgggct tggtgctgaa aatcggaaag gataaatcat acgatgttca agaaaagaag
      841 acggcatgtc catcacacgc aaccggttca tggcaatctc gacaaaaatg agcgttttat
      901 caagcgccaa tgttgcattt atctggagcg cctgctggcc gtctttccat tgacggacag
      961 agatacgttg gagatgttaa catatgttct ggggccgaaa atatccgagc ttaagaaaac
     1021 tatcagtaaa aaagtttatt ccaagcaacg tcacacacca gtttttgaca cggatgatga
     1081 actggatatc ctggatgatg attccgacga cttctaccag atgatcaagc acgcgggaaa
     1141 gaaaattcaa aaagaattca tcacactcag tcgaaccgtt ctggcgaaac gtgtcaaaac
     1201 gctatcttat gcaggcaaaa gcgaaatgga aaagaaactc gatactttgg ataaaatgtt
     1261 cgctttgtcg aatgaggaaa aggcctttac tacattgctt tacatcaata cggtctggcc
     1321 gcgtgccgac gagtattttg tggatcattt gcactgccag gatattttcg gcaggcgtta
     1381 tctgaaggtg atgctgcaga ttaccgactc gggaatcaac cggattctgt ccggcacgct
     1441 ggcgcgtatt gaattctttg aatttagtaa tcattctttc aatctgagcg atgattattt
     1501 gggatttttt cagaaaccat taaaagatgt gctgaaaaag aaggattttg tccgcttttc
     1561 gggcaaaacc attcctctga agaatcacct gattgatccc ggcgttacca aacatatcct
     1621 tagccttctc agaaccaaac gggaatcatc aaaccatatc ctgctttatg gtcctccggg
     1681 cacggggaaa acaagttatg ctctgggttt ggtcaataaa ttaaaaattc ccgcctacgc
     1741 cattatggac gattcatgta atcagacacg gacgcgcagg gcggcgatta ttgcctgccg
     1801 gaatatgacg aacgatggcg aaggatcttt aatcatcgtt gatgaagcgg acagtctgct
     1861 caataccgag gagtcatgga gctccggcgg cgaaataaag gacaagggct ggcttaatca
     1921 actgctggaa gagccgggtg cccgaatgat ctggattacg aacagcattg aagatatgga
     1981 agattccgta ctgcgccgtt ttgcctttag tgtccatttt ccggcattca accggagaca
     2041 gagattctcg ttgtgggaat cagtgctttg tcgccaccat gtccgaaaac ttttcaaaga
     2101 tgatgagatc aacaaactga cggcgcgcta tccggtcagc gcggccatca tcaatatggc
     2161 cgttaccaaa gcgctggaat cagcagcggc tgatcacatt gcctttaaag aaatcatgat
     2221 catgaatctg gatgctcatt ggaagctttt aaacggcggc gcaaagccca aaaccggcga
     2281 ccgcattgaa gaacattatt ccatcgaagg tttgaacatc gaaggaaatc tgccggttat
     2341 cctggcgcag ctggactctt ttgaccgctg cctgcgtgcg cctgataaga gcggcgtccg
     2401 caacttcaat ctcctttttt acggaccgcc cggcgcggga aaaagcgaat tggcccgcta
     2461 tgtcgccaag cgtctggaca gagagttaat ggttaagcgc gccagtgata ttgtcagccc
     2521 ttatgtaggc gaaacagagc agaatatcag ccgggtgttt tccgaggcgg aatcaatgga
     2581 agcggtgctg gtgattgacg aggcggattc tttccttttc agccgcgacc gcgctgttcg
     2641 ctcctgggaa atcagccata ccaacgagtt tttgacgcag atggaaagat tccggggcat
     2701 tttgatctgc acaaccaacc gctttgccga tctggatatg gcttccgtcc gccgttttaa
     2761 tcaaaaaatc ggatttggct gtctgaaacc ggacggtaat atgatcttct accaaaaatt
     2821 gctttgcgaa ttatgcaaaa caccagtgaa caggaaccag gaagagctga ttggacgtat
     2881 ccatgatctg acgccgggag acttccgtat tgtgcgagac cgttttgcga tcagtgatga
     2941 aaaatgtgtg acgcacgagc aaatgatcca ggcgctggag gcagaagctc gcgtcaaaaa
     3001 aacacaacaa ggcaacaggc cgattggttt tatgcggcgt accgcatagg cgaatacaaa
     3061 gaagtcaatc aacaatcagt tttatagttc ttaatcatgc gggattattg atggaagaag
     3121 gcaagcattt tatcggtgtg ggtgacaccg gacacagagc ggcaaaggaa ttatgtcgga
     3181 tttacgagga aatgcgggga tgtctttttg aatcagaaca ggacaggcca cacaaccatt
     3241 tgcgtattca ttcctatccc tgtgccgaaa cggcctgttt tgatgataaa gatcatcttg
     3301 tcattctagt ggggtcgctc atcgatccgg gtttgcagga tgcgcggacg tcttttcatg
     3361 agagcaggcc atattttatg ttgacggttg gtattgatca tcagacaaga gtggacgctg
     3421 acgacattca gccactacct gatgaatgtt tgatttatcc tgacacgtct ctttctgttc
     3481 ctgtcaaact ggcgcaactg gttttgcaaa tctttttgat ccacacgcca tggtacataa
     3541 gcaaaagagg tgcgctgatc ggttatgatc tttacgacac aaagcagtta tttggcggca
     3601 aagttgtaaa agccatatcc atgacatctg atagggcaca ttaccggcaa aacttctcca
     3661 tgtttcttga caaaaataga gccgacatca ggcggacaaa gggcatcctg atgtcggtct
     3721 gggggctgtc tataatgcag gcaaatgaga ttttgaagga aacaagattg ctgctgacgg
     3781 ctggggcaga tgaattgttt acgtgtcata atctgccagc ggatgagccg aatattttgg
     3841 taacgctgtt tcttgcattt taaaacgatt ttttcggggt actgtactga tgacaacgga
     3901 aatgatgaca gagccaatca taaaatattg ccgggacaag attcaacggc ggttgaattt
     3961 aagcattacg ccggacatac agatacggat tagaaatttt gatggaccgg atcgctgcgg
     4021 tcattacacg atccagccga atgcatggaa cgtcattgaa ccggctgaat tctggtgtgt
     4081 aaccggatat tttaagaagg ttatcgggca gctccgcaaa gaataccctc agttgcataa
     4141 ggataccgat tggcggattc ctctgaagcc tttcctgatt gaaccgatta tcagttttgg
     4201 cagttcggcc atgaaggtca caggcggcat caactggctt gtcatgggcg tattgaagaa
     4261 caaagagaaa ttctgtgccg ttttagactt ggagatgcgc ccgctgtttc ggaaatgtgg
     4321 gctggcaaat cttgtaaagc atacggaatt ggaattggcc aggcgtgaga aatgtgattt
     4381 cattcagaca tggcattggg ctgccaatcc taattttaat gcggccatcg cacccggttt
     4441 aaaaagtgga ttcatcctgt atcatgggca accacaggat ggcgacgtat atgaagaccg
     4501 gggccatgtc catctgcggt attactttga tgaaacaaaa aaacgctctg tggaggtaaa
     4561 aaccaaagac ggcaaagtat tcatgagtcc gatcgataat cacgccatca ttgattatct
     4621 ggaagcttgc ccgaataagt atcccggtcg gatgattcag agcattgagg aatacagcaa
     4681 gggaaaagca atgacgccga ttaagaagaa caagcgcatt gcggaaaaac acaacggggg
     4741 ctcagaacgg caaagaatat ttattgccca aggcgcggcc ggatttgaat atacaagaca
     4801 aagaaataca tacaggatcg gcgatagcat ctcattttat cccatccttc agattgatca
     4861 ttttaagaaa agcagccaaa atgcgcctta cctgaagcat gtcgtctata atatctatga
     4921 gttttgcttc aaagtggatg ccgtgcaacg attggaagag acccacggga tgatgcgcaa
     4981 tgattacctc atggaactgc tgcccaactt caaggtatgc tgccgcgatt ggaataaaga
     5041 gaaccaattc atcgttgata aattctatga gggatatggc aggctgacca tcggtgatca
     5101 ccgtagctca atgcattata agcagcctcc ccatcagcaa aatattttga tgaagggtaa
     5161 attggtcggt atttcggatg acttgatgca aagagacttc tcaacgaaat atgcccacca
     5221 ggttaagcgc ttccgttatg gcaaagatgt ttatggtgat gaggatgctc tggaatggtt
     5281 cggctattac cgggacttga agaaattcaa gaaaaacgtg atttcttata caacgaaccc
     5341 ggtggaatat gaatccacag attcatttgc actgtacggc tacagtccaa aactaatttt
     5401 ctgtattgag attatagcgc cacaataaat
//
LOCUS       DAOSME010000108         5227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_17284_length_5227_cov_16.935422, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000108 DAOSME010000000
VERSION     DAOSME010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17284_length_5227_cov_16.935422"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLG98_10735"
     CDS             <1..640
                     /locus_tag="PLG98_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE33781.1"
                     /translation="TSAQDGIKIVRMIRKNAALAAIPAVMIVSTSFQDDTKTVAEKLG
                     VKYFLKRPFHRSNLLDAMMNIFGQTIKKVSPAVRNPVKAPATEALAGQRILLVEDNFI
                     NQQVAREILQQAGLKVVVAENGKDALGKIRQEQFDLVLMDVQMPEMDGYQATKIIRRN
                     KKFKSLPIIAMTAQAMSGDKEKCLNAGMNDYIAKPISIQNTLSVLSRWLYSK"
     gene            811..1710
                     /locus_tag="PLG98_10740"
     CDS             811..1710
                     /locus_tag="PLG98_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE33782.1"
                     /translation="MISQEAKNVMNLMSTMKLPDAGELPIELCRRVLADMMSLSLPPA
                     GCRFEPVNAGGPEALWVKAVSADDTAVILYLHGGGYAMGSPQTHAGFTGLLSELSRLS
                     VLSVDYRLAPENPYPAAIEDSLSAYCWLLRQGVPAESIVVGGDSAGGGLTFATMLKLK
                     EREEALPAAVFAVSPWVDLAVTGESIEINAARDPMITRSGLDYMAGLYAGHADVRLPF
                     ISPLYADLRGLPPVLIHVGTCEMLLSDSLRIAASLENSGVDCTLRVWEDLFHVFHSVV
                     SLPEAIMATEDIAAFVRNRVGGY"
     gene            1741..>2339
                     /locus_tag="PLG98_10745"
     CDS             1741..>2339
                     /locus_tag="PLG98_10745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE33783.1"
                     /translation="MKKETLVLKTTDGMQMFVYKWTPDGADAVKGAVQIAHGMAEHAA
                     RYERFGGALTEAGYAVYANDHRGHGRTAGSPDNRGYFADENGWAKVVEDMHLLTGLIK
                     KDYPQKPFFLFGHSMGSFLSRHYAMLYGRELNGLILSGTGGDPGVAGRAGLFIARMDA
                     RLHGKKAKSEIMNKLSFGAFNHAFKPNRTEYDWLSRDHA"
     assembly_gap    2340..2439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2440..4178
                     /locus_tag="PLG98_10750"
     CDS             <2440..4178
                     /locus_tag="PLG98_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33784.1"
                     /translation="MATQIPRKRTIFFIRLMVVITTAYFILLSPVEGKELEIYGYIFI
                     AVYLMTNLIVAYIPEKYFHDNRIFYGFILCDSILLPAGIYFSGYIGSDLYLMFFFIIS
                     LTTMSSRFGYLMINTFLFCLIYLWLLHEKGVLTGPKAGAYLIQIPFIIVIAIFYGYLI
                     TTRLKDKDMRINEVRQRYEQVVQATDVLMSIVDRDGKLKFANDIFARFYGYHDDKRLA
                     GLTIAQVNAEDASEADKLLDYVQSVYQKNDMVRYESYNKKHNLWFANTLGPIRDPSTG
                     DVLDVCVISKDITDRIEKEKKLNNTVELLIKTRDQLIQQDKMAALGRMASGIAHEIRN
                     PLEIIYMGIDYLENNIAHQDPQIDGSIEKIYKAANRADNIINNILSFSRKSEYKITQI
                     PVCQLLDNTLVLANQALHKGHVTVKREYETESLEAAGDYNMLEQVFLNLINNAIDAMK
                     ESTEKRLTIRASGKIISEIGYKTGYRYTDFFRIGEKKIVVEISDTGKGMSKDVQSKIF
                     EPFFTTKSVNEGTGLGLSIVHMIIERLSGTIDVASEENKGTTFYVNIQPQIKKVVAEE
                     EQHDKSDQSSGH"
     gene            4147..4536
                     /locus_tag="PLG98_10755"
     CDS             4147..4536
                     /locus_tag="PLG98_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE33785.1"
                     /translation="MTNQIKVLAIDDEADFCYFIKKNLTQSGLFDVVVANNGADGIKL
                     ARKEKPDIILLDLFMPDIPGEDVAAELKDHPATADIPILYITALASNDEIVEGESNKM
                     GDNYIMAKPVRTKKLIETIMKILGQSY"
     gene            complement(4645..>5227)
                     /locus_tag="PLG98_10760"
     CDS             complement(4645..>5227)
                     /locus_tag="PLG98_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE33786.1"
                     /translation="FKYRPRKIPDFTMLDDGSVIEIGDYELQCILTPGHTQGHICLYE
                     EDKQILFSGDHILYDITPHIESWSYEVNSLENYMQSLDKVRDLPVKLVLPGHRNFFTD
                     FKGRIDELKNHHEMRAKEVLDVLGKNTMSAYDIAAGMTWNIDCESWEQFPVAQKWFAV
                     GEAIAHLLYLESRGKVKRHPGEKTDLFSAIRME"
BASE COUNT         1370 a         1156 c         1326 g         1275 t
ORIGIN      
        1 aacgtcggcc caggacggca taaaaatcgt caggatgata agaaaaaatg ccgcgttggc
       61 cgcgattcct gcggtgatga tcgtttcaac atcctttcag gatgatacca aaaccgttgc
      121 tgaaaaactg ggcgtgaaat atttcctcaa aagaccattt caccggtcga atcttttgga
      181 tgcaatgatg aacatcttcg gccaaacgat caaaaaagtc tcccccgcgg tccgtaatcc
      241 ggtcaaggct cctgcgacgg aggcgcttgc cggccagcgg atattgctcg tggaggacaa
      301 cttcatcaat cagcaggttg cccgtgaaat tcttcagcag gcggggctga aagtagtcgt
      361 tgccgaaaac ggaaaggacg ccctgggtaa aatcagacaa gagcaatttg acctggtgct
      421 tatggatgtc cagatgcccg aaatggatgg ataccaggcg acgaaaataa tccggcggaa
      481 taagaaattc aaatcgctgc ccatcattgc catgacggct caggctatga gtggcgacaa
      541 ggaaaaatgc ctgaacgcgg gaatgaacga ttatatcgcc aaaccgatca gtatccaaaa
      601 tactttaagc gttttaagcc gctggctgta ttccaaatag agattcatgg tatcggaaac
      661 gatggctgac ggaggggaaa aggttctgtc catcggttga gattggcgga taacaaacca
      721 cagataaacc atagcgtatg acattgaaaa aggcgatacg tctgttatat tttttttcat
      781 gatacggaca tcggtccggg aggatgtgcc atgatcagtc aggaagcaaa aaacgtcatg
      841 aatctgatgt cgacgatgaa actgcccgac gccggcgagt tgccgataga gctctgtcgg
      901 cgggttctgg cggacatgat gtccttgtct ttgccgccgg ccggatgcag attcgaaccg
      961 gtcaatgccg gaggaccgga ggcattatgg gtaaaggctg tatcggcgga cgatacggcg
     1021 gttattttat atttgcacgg aggcggttat gccatgggct ctccgcagac ccacgcggga
     1081 ttcacggggc tgttgagcga gttatcccgc ctgagcgtat tatccgttga ctaccgtctc
     1141 gctccggaaa atccctatcc tgccgcgatt gaagattcgc tctcggcata ttgctggctt
     1201 ttgcggcagg gtgtgcctgc ggaatcgata gttgtcggcg gggattccgc cggaggggga
     1261 ctgacgtttg cgacgatgct gaagttgaaa gaacgggaag aagctctccc ggccgcggta
     1321 tttgccgttt cgccgtgggt ggatctggcc gtgaccggcg agtccattga aataaatgcc
     1381 gccagggatc ccatgatcac gcgatccggt cttgattaca tggcgggcct gtatgcggga
     1441 catgccgatg tgcgcttgcc gtttatttct cctctatacg cggatctgag agggctgccg
     1501 ccggtgctta ttcatgtagg aacctgcgaa atgctcctga gtgactcgct ccgtattgcc
     1561 gcttccctcg aaaattcagg cgtggactgc acgctccgcg tatgggagga tctgtttcat
     1621 gtttttcatt cggtggtcag tcttcccgag gcaataatgg ccacggagga cattgccgct
     1681 tttgtcagga atcgtgtggg cggatattaa ccttgaataa tgatgtgcag gagaggaaaa
     1741 atgaaaaaag agacccttgt tttgaaaaca accgacggga tgcagatgtt cgtttacaag
     1801 tggacgcctg acggcgcgga tgccgtgaag ggggctgtgc agatagcaca cggcatggct
     1861 gagcatgccg cacgttatga acgttttggc ggcgccctga ccgaagcggg atatgccgtt
     1921 tacgccaatg atcaccgggg gcacggcaga accgccggat cgccggataa tagaggctat
     1981 tttgccgacg aaaacggctg ggcaaaagtt gtagaggaca tgcacctgct gaccggcctt
     2041 attaaaaagg actatccgca aaagccgttc tttctcttcg gtcacagcat gggatctttt
     2101 ctttcccggc attatgccat gctttacgga cgggaactca acggccttat tctgtcggga
     2161 acgggcggtg atcccggcgt ggcaggtcgg gcggggcttt tcattgccag gatggatgcc
     2221 aggcttcacg gcaaaaaagc gaaaagtgaa atcatgaaca aattgtcgtt tggcgctttc
     2281 aatcatgctt ttaaacccaa ccgcacggaa tacgactggc tttcccggga ccatgccgan
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt catggcgaca cagattccaa
     2461 ggaaaagaac gatatttttt atccggctga tggttgtgat taccacggcc tattttattc
     2521 tgctgtcacc ggtagagggc aaagaactgg aaatttacgg atacatcttc atcgccgttt
     2581 acctgatgac caatctgatt gtcgcgtata tcccggaaaa atattttcac gataacagaa
     2641 ttttttacgg ttttatctta tgcgatagta ttcttcttcc ggccggaatt tatttttccg
     2701 gatatatcgg atccgactta tatctgatgt ttttctttat tatttcattg accaccatga
     2761 gctcgcgttt cgggtatctg atgatcaata catttttatt ttgtctcatc tatctctggc
     2821 tgctgcatga gaagggggtg ttgaccggtc cgaaagccgg cgcttatctg atccagattc
     2881 cattcatcat cgtcatcgca attttttacg gctatctgat cacgacgcga ttaaaagaca
     2941 aggacatgcg gataaatgaa gtccggcaga ggtatgaaca ggttgttcag gccaccgatg
     3001 tcctgatgag tattgtggat cgcgacggaa agttaaaatt tgccaatgat atatttgccc
     3061 ggttttacgg ttatcatgac gacaagcgtc ttgccggact gaccattgcg caggtgaatg
     3121 ccgaagatgc atccgaagcg gataaacttc tggattacgt gcagtcggtg tatcaaaaaa
     3181 acgacatggt gcgatacgaa tcctataata agaaacataa tttgtggttt gccaatacgc
     3241 tcggtccgat cagggatccg tcaaccggtg atgttctgga cgtctgcgtt atctcgaaag
     3301 atatcaccga ccggattgaa aaagaaaaaa aattgaataa cacggttgag ctgttgatca
     3361 aaacgcggga ccagctgata caacaggata aaatggccgc tctggggcgc atggcgtcag
     3421 gcattgccca tgaaatcagg aatcctcttg aaattattta catggggatc gactatctgg
     3481 agaacaatat tgcccaccaa gaccctcaga tcgacggctc catcgaaaaa atatataaag
     3541 cggcaaaccg cgccgacaat atcataaaca acattctgtc tttttccaga aaatcggaat
     3601 acaaaattac gcagattccg gtctgccaat tgctcgacaa cacgctggtg ctggccaatc
     3661 aggcccttca caagggacat gtcactgtga agcgcgagta tgaaaccgaa tcgctggagg
     3721 cggcgggcga ttataacatg ctggagcagg tttttctgaa tcttatcaac aacgccattg
     3781 acgcgatgaa ggagagtaca gaaaagagat tgaccattcg cgcctccggg aaaatcatat
     3841 cggaaatcgg ttacaaaacc ggttatcgct atacggattt tttcagaatc ggcgagaaaa
     3901 agattgttgt agagatatcg gacaccggaa agggaatgtc caaagatgtt cagtcgaaaa
     3961 tattcgaacc gttttttacc acgaaatccg tcaatgaagg aaccggtctg gggctcagta
     4021 tcgttcacat gatcatcgag cgtttgagtg gaacgatcga tgtcgccagt gaggagaata
     4081 agggaacgac attttatgtc aatattcaac cgcagattaa aaaggtagtt gccgaggagg
     4141 aacaacatga caaatcagat caaagttctg gccattgatg atgaagccga tttttgttat
     4201 ttcatcaaaa aaaatcttac gcagtccggt ctgttcgatg tggttgtggc aaacaacggc
     4261 gcggatggaa taaaactggc cagaaaagaa aagccggaca tcattctgct ggatttgttt
     4321 atgcccgaca ttccgggtga agatgttgcc gctgaattga aagaccatcc tgccacggcc
     4381 gatatcccga ttctctatat tacggctctt gcgtccaatg acgaaattgt tgaaggcgaa
     4441 agcaataaaa tgggagataa ttacatcatg gcgaaacccg tcagaaccaa aaagctgatc
     4501 gaaaccatta tgaaaatact ggggcaatcg tattagcggc ctaaaacatt cagaatacag
     4561 aaacaatcaa cagaaaaaaa ccacaggaag ggcggtttgc tctgagcggt ctgtgccgga
     4621 aaatacaata agccggacaa aaaatcattc catccggatt gccgaaaaaa gatccgtttt
     4681 ctcgcccgga tgtcttttta ctttgcctct gctttccaga tagagcaggt gcgcaatggc
     4741 ttccccgacg gcaaaccatt tctgggccac cggaaactgt tcccaacttt cgcaatcgat
     4801 attccaggtc atgcctgcgg caatatcata cgcactcatg gtatttttgc ccaaaacgtc
     4861 aagtacttct ttagccctca tttcgtgatg attcttcaat tcgtctatgc gtcccttgaa
     4921 atcggtaaag aagttgcggt gtccgggcag aacaagtttg accggaagat ctcttacctt
     4981 gtcgaggctc tgcatatagt tttccagaga attcacttcg tacgaccagg attcgatgtg
     5041 cggagtaata tcgtaaagaa tgtgatctcc ggaaaatagt atttgcttgt cttcttcgta
     5101 caggcagata tggccttggg tatggccggg ggtaagaatg cattggagtt cataatcgcc
     5161 gatttcgatc acgctgccgt cgtccagcat ggtgaagtcg ggaattttcc tcggtctgta
     5221 tttaaat
//
LOCUS       DAOSME010000109         5109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_17791_length_5109_cov_18.682825, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000109 DAOSME010000000
VERSION     DAOSME010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17791_length_5109_cov_18.682825"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLG98_10765"
     CDS             <1..366
                     /locus_tag="PLG98_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE33787.1"
                     /translation="ADMGVLQRLPKIVGQGFAREMAYTAANYTARDVEKKGLLNGIYA
                     DQESLMAAAEKLAAQIAANAPLGIKYTKEVLNFSRYVNVYEGMALAVQKNAILLMSED
                     LREAMMSFLERRPPDFKGK"
     gene            complement(503..1525)
                     /locus_tag="PLG98_10770"
     CDS             complement(503..1525)
                     /locus_tag="PLG98_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HOE33788.1"
                     /translation="MKKLIFQLLEKKGRVATADLVNKTGLSRAYAQRFLKELADEGVI
                     LLIGKANQAHYVLTPEKGIPLPPRVRKVLLNKGLSEDKVLDAVKSESSVFQGISGNVS
                     SIVNYAFTEMLNNAIEHSASDKIDLVVLKTSTHIRFTVTDRGVGIFNNIMNQNKLAST
                     MDAIHDLLKGKETTAPQAHSGEGIFFTSKIADQLTIASFEKKIVFDNVGRDVYIRDIK
                     SARGTRVDFALSLTSENTLSDLFHQYTDESFQFSKTGVRVKLYHRGVEYVSRSQARRI
                     VAGLEKFKMIELDFEGVETVGQAFADEIFRVWQNAHKGIKVIIKNTNENILFMIRHAQ
                     HSLTTG"
     gene            1868..>2374
                     /locus_tag="PLG98_10775"
     CDS             1868..>2374
                     /locus_tag="PLG98_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33789.1"
                     /translation="MKRNQWTPYCHLPRRKQRDSFIRLRQKIRNRIAVDGGQFLSRHI
                     LDEPGRPAIFNQWADVYFLGGNGLTIWNASIVTAAHEFWETVEEMAHSRAWEMLTPEE
                     QQAEAEMKFEPIWYHGKRMYRMLERPVLTYDKFGGLTFRDYQEKINEEIIKNEPPAIF
                     ESFTLDRSY"
     assembly_gap    2375..2474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2475..2673)
                     /locus_tag="PLG98_10780"
     CDS             complement(<2475..2673)
                     /locus_tag="PLG98_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33790.1"
                     /translation="MNDSISLLTGLIFRKALLSDCLGLRSVNFIYITPAAICLLPFRV
                     DGQHQQSIDRTDISLLPSNPAS"
     gene            2684..>5109
                     /locus_tag="PLG98_10785"
     CDS             2684..>5109
                     /locus_tag="PLG98_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33791.1"
                     /translation="MLLLDKISVIESQPKDESEEHLKNDLRDFLRDTYYRDSNAINTK
                     DKKDLVIHLGKTTDSEVGVIIEAKRPSNKSEMLTKDNINVKAFHELILYYLRERITQK
                     NLQIKYLIATNIYQWFIFDAQIFDKLFAQNKIFVEQFQNIEGKRLSDNRTVAFYEEIA
                     KPFVNNIEAEITFTWFDISDFEKPLRNADRRDDNSLIALYKLLSPQHLLKLSFANDSN
                     SLDKSFYTELLHIIGLEETKEGGKRLIGRKAAGSREQGSLMENAITQLDSLDRISRME
                     KPQRYGETDSERLFNLALELSITWINRLLFLKLLEAQLIKYHKGDKSFAFLNIEKVTN
                     FDELNSLFFQVLARKPEERSEQVKKMFANVPYLNSSLFEPTESEHNGLFISMLKDAPL
                     PVLNSTVLKDQAGRKQTAQRGTLEYLFEFLDAYDFADDGAEEVREDKKTLINASVLGL
                     IFEKINGYKDGSVFTPGFITMYMSREAMRRAVVQKFNAKYNWKAANIGELKNYLADRN
                     SSREKLEFNAVINALRICDPAVGSGHFLVSALNEIILIKYELGLLVDDQGARLSDYEV
                     TLENDELIVTDENGKIFEYNPLKKESCRIQKTMFHEKQTIIENCLFGVDINPNSVNIC
                     RLRLWIELLKNAYYKAPDFTELETLPNIDINIKCGNSLISRYPLDADIKNALRNSKWN
                     VNGYRLAVMAYRNAKSKDDKKAMEKLIEEIKSNFESEVAAGDKRVVRLKNLKDELKTH
                     ATQTHIFELSKAERAAWEAKLKKLTAEIKKLETTLEEVKNNKIYENAFEWRFEFPEVL
                     NDDGEFVGFDV"
BASE COUNT         1420 a         1153 c         1168 g         1268 t
ORIGIN      
        1 gcggacatgg gcgtattgca gagactgccg aaaatcgtcg gccagggctt tgcccgcgaa
       61 atggcctaca ccgcagccaa ctacacggcg cgggacgtcg agaaaaaggg gcttctaaac
      121 ggcatttatg cggatcagga aagtctcatg gccgcagccg agaaactggc cgcgcagatt
      181 gccgccaacg cgccgctggg catcaaatac accaaagaag tcctcaactt cagccgttat
      241 gtgaatgttt acgaaggcat ggcgctggcc gtccagaaaa atgccatcct gctcatgtcg
      301 gaagacttga gagaagccat gatgtctttt ctggaaagac gcccgccgga tttcaaggga
      361 aaatgatgct gattcctgtt ttgcagggaa cggtcgcgac cgttccctac cttttcagcg
      421 tccggagcgt cttgatgtcc atattccgga gttccatttc cgtgcggttg acaacttttc
      481 tgaaaatcca ttataaaagt tgtcaaccag ttgtcaacga atgttgtgcg tggcgtatca
      541 tgaacaatat gttttcattg gtgtttttta taattacctt gatgcctttg tgcgcattct
      601 gccagacgcg gaagatttca tcggcaaagg cctgtcctac ggtttccacg ccttcgaagt
      661 caagttcaat cattttgaat ttttccagcc ccgctactat tctgcgggct tgcgaccggg
      721 aaacgtattc aaccccccgg tgatacagct tcaccctgac gccggtttta ctgaactgga
      781 atgactcatc ggtatattga tggaaaagat cggagagcgt gttttccgat gtaaggctca
      841 gggcaaaatc aactcttgtc ccccgagcgc tcttaatgtc tctgatataa acatcccggc
      901 cgacattgtc gaaaacgatc tttttttcaa agctcgcaat cgtcaactgg tcggctattt
      961 ttgatgtaaa gaatattcct tcgccgctgt gcgcctgcgg cgcagtggtt tctttcccct
     1021 tgagcagatc atgaatggca tccatcgtcg aagccagttt attttgattc ataatgttgt
     1081 taaaaatacc aaccccccgg tccgtcaccg taaagcggat atgcgtggag gttttcagca
     1141 ccaccaggtc aatcttgtcc gacgcggaat gttcgatggc attgttcagc atttcggtaa
     1201 atgcgtagtt gacaatggat gacacattgc cggagattcc ttgaaaaacg gaactttcac
     1261 ttttgacggc gtccagcacc ttgtcctcgg acaacccctt gttgagcaac acttttcgaa
     1321 ccctcggggg caacgggatt cccttttcag gggtgagaac ataatgcgcc tggtttgcct
     1381 tgccgatcag aagtatcacg ccctcatccg ccagctcttt gagaaatctt tgcgcatagg
     1441 ctctggaaag ccccgttttg ttcacgaggt ccgccgttgc aacacgcccc tttttctcca
     1501 gcaattgaaa tatcagtttt ttcatgtcca tagcgttcgc cctgaaggat ggtttacgac
     1561 tttattgtaa acaaatttaa aaagttgtca acaagttgta tgtccttaaa gagtcctgcc
     1621 gcaatatatt gggcgggcgc tcgcggacgc aaccgctccc tattggaatt ccggatcatc
     1681 tgttgacgat gttgacaaat tgtcgaggcg tgattttggg ccgaattgcc tcaaatctat
     1741 ttttttaaac ttgattcgct tggtgttgta gtgcattgta gcgcaggagt tgcaggatgg
     1801 gagcggtgtt ggacaccaat gttctgattg ccgccttttg aaaatcgttg tatgaaatca
     1861 aacgcctatg aaacgtaatc aatggacgcc atactgccac cttccgcgcc gcaaacagcg
     1921 cgacagcttt atccggcttc gtcaaaaaat acgaaaccgc attgcggttg acggcgggca
     1981 atttttaagc cgccacatcc ttgacgaacc gggccgccca gccatcttca atcaatgggc
     2041 cgatgtatat tttttgggcg gcaacggctt gaccatctgg aacgcatcca ttgtcaccgc
     2101 cgctcacgaa ttttgggaaa ccgtcgagga gatggcgcac agccgtgcct gggaaatgct
     2161 taccccggaa gagcagcagg cggaagcgga aatgaaattt gaacccatct ggtatcatgg
     2221 caaacgcatg taccgaatgc tggaaaggcc ggtgctcacc tatgataagt ttggtggtct
     2281 gacctttcgc gactatcagg aaaaaatcaa cgaggaaatc attaaaaatg agccgccggc
     2341 catctttgaa tcgtttaccc tggaccgctc ttatnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnncggaag caggatttga aggcaataaa gaaatatctg tgcggtcaat
     2521 agattgctga tgctgtccat cgacacgaaa aggcaaaagg cagatggctg ctggagtaat
     2581 ataaatgaaa ttgacggaac gtaatcccag gcaatcgctt aataaggctt ttctaaaaat
     2641 taaacccgtc agaagcgaaa ttgaatcatt caagacaaat cttgtgttat tgctcgacaa
     2701 aatcagcgtc atcgaaagcc aacccaagga tgaaagcgaa gaacacttaa aaaacgatct
     2761 gcgtgatttc ctgcgcgaca cttactaccg tgacagtaac gccatcaaca ccaaggataa
     2821 aaaagattta gttattcatt taggtaaaac cactgatagt gaagtcggtg ttattattga
     2881 ggccaagcgt ccgtccaaca aatccgaaat gctgaccaaa gataatatca acgtcaaagc
     2941 ttttcatgaa ttgattttat actacctgcg tgaaagaatt acccagaaaa atctgcaaat
     3001 aaaatatctg attgccacca atatttatca atggtttatc tttgacgccc agatatttga
     3061 caagctcttc gctcaaaata aaatcttcgt tgaacaattt cagaatattg agggcaagcg
     3121 tttatccgat aaccgaaccg ttgcgtttta tgaggagatt gccaagccat ttgtcaataa
     3181 cattgaagcg gaaataactt ttacctggtt cgatatcagt gactttgaaa agcccctgcg
     3241 caacgcggac aggcgagacg acaacagcct gattgcgctt tacaaacttc tctcacccca
     3301 acacctgtta aaattgtctt ttgcgaatga cagcaacagt ctggataaaa gcttttatac
     3361 cgagttgctg catattatcg gccttgaaga aacaaaagaa gggggcaaaa gactgatcgg
     3421 tcggaaagca gcgggcagtc gtgaacaagg ctctctcatg gaaaatgcca ttacccaact
     3481 ggatagtttg gatagaattt cgcgaatgga aaaaccgcaa cgctatggcg aaacagatag
     3541 cgagcggctg tttaatctgg cactggaact ttccattacc tggatcaacc gtctcctttt
     3601 tttaaaactc ctggaggcgc aactcatcaa gtaccacaag ggggacaaat cattcgcgtt
     3661 ccttaatatc gaaaaagtaa ccaattttga tgaactcaac agccttttct ttcaggtgtt
     3721 ggcgcgtaag ccggaagaac gaagcgaaca ggttaaaaaa atgttcgcca acgttcccta
     3781 tctgaacagt tcactttttg aaccaacgga aagcgagcac aatggtttat tcatcagcat
     3841 gctgaaagat gcaccgctgc ctgttctcaa ttccaccgtc ttaaaagatc aggcaggtag
     3901 gaaacagacc gctcagcgcg gcacactgga atatttgttt gagtttcttg acgcttatga
     3961 tttcgcggac gacggtgcgg aagaggtcag ggaagataag aaaaccctga tcaatgcttc
     4021 agtcctgggg ttgatttttg agaaaatcaa cggttacaaa gacgggtctg tttttactcc
     4081 cggctttatc actatgtata tgtcccgcga agcaatgcgc cgggcggttg ttcagaagtt
     4141 caatgcgaaa tataactgga aagccgccaa tatcggagag ctgaaaaatt acctggccga
     4201 tagaaacagc agtcgggaaa agttggagtt taacgcagtc attaacgccc tgcgcatctg
     4261 cgaccccgcc gttggaagcg ggcacttttt agtttcggcg cttaacgaaa ttattttgat
     4321 caagtatgaa ttggggctgc tggttgacga tcagggcgca cgactatcgg attatgaagt
     4381 cacccttgag aacgacgaat tgattgtaac agacgaaaac gggaaaatat tcgagtacaa
     4441 cccgctgaaa aaagaaagct gccgcattca aaaaacaatg tttcacgaaa agcagaccat
     4501 cattgaaaac tgtctgtttg gcgtggatat caatcccaac tccgtcaata tttgccgtct
     4561 tcggttatgg attgaactgc tcaaaaacgc ctactacaaa gccccggact ttaccgagct
     4621 ggaaaccttg ccgaatatcg acattaacat caaatgcggc aactctctga tcagccgtta
     4681 tccgctggac gctgatatta agaatgcctt gcggaatagc aaatggaacg ttaacggcta
     4741 ccgccttgcg gtcatggcct atcgaaacgc caagagcaaa gatgataaaa aggctatgga
     4801 aaaactcatt gaggagatta agagcaactt tgaaagtgaa gttgccgccg gtgacaagcg
     4861 tgttgtccgg cttaaaaatt taaaagatga attaaaaact catgccacgc agacacatat
     4921 ttttgaactt tccaaagccg aaagagcggc atgggaagca aagctcaaaa agctgactgc
     4981 ggaaatcaag aagctggaaa ccacccttga agaagtcaag aataacaaaa tttatgaaaa
     5041 cgcttttgaa tggcggtttg aatttcctga agtgctcaat gacgacggtg aatttgtcgg
     5101 gtttgatgt
//
LOCUS       DAOSME010000110         5052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_18063_length_5052_cov_26.790875, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000110 DAOSME010000000
VERSION     DAOSME010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18063_length_5052_cov_26.790875"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLG98_10790"
     CDS             complement(<1..274)
                     /locus_tag="PLG98_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33792.1"
                     /translation="MYKKNAGQVVFVVMFIILMVSGIAAAQSAGRNDGASNSGEACYK
                     CHFQIKSLKEGSKHAMLSCAVCHSGTKEHVQSFRNKPVTAIDQAVCG"
     gene            complement(555..2066)
                     /locus_tag="PLG98_10795"
     CDS             complement(555..2066)
                     /locus_tag="PLG98_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOE33793.1"
                     /translation="MHASWMKMSVVGLLAFVLVVTACGGRDSLTVSPGTGMLALARQA
                     KARGEFTTATGLYRQARTLLTNEGKNDAALECLNSLRDIQLITAQYPYRLGETRAMLA
                     DAFPQVTPEEREAWITQGKLESTMIDGESRFFSDFISNVKFRNVDLFRQDANMYKGFE
                     DHYKLLKQVVDAGPGADPWQPFIRPISYTATCTLAVPRGKLPAGGILKLWFPIAIVTG
                     PQRNVSVLSITPMTYVKGSPSIDQDIGLIYMEVPLSELTQDLHIVIRFTFDHYEQWFQ
                     IDPDRVGAYDRKSDLYLKYTASCGNIAVTNEIRRTAQTVIGGETNPYLAAKKIYDYVL
                     ATVTYSYMPHLALWPRGEPESVYVHRNRYGDCGAQSMYFSALCRSVGIPARTTGGWQL
                     FTGNLSGHFWAEFYLPNYGWIPVDPTAADMAGYLTDVSDEDKKAFQDFFFGNQDHFRC
                     NVQKDVDVPLIPPSRERPFMTSSIQYPAATCDTMEDDPGIMIMDYWSTTVSVN"
     gene            2953..3543
                     /locus_tag="PLG98_10800"
     CDS             2953..3543
                     /locus_tag="PLG98_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE33794.1"
                     /translation="MMENFLLITLLSEKYKMDELKNGTLRTDRLELTAATFDHIHAEM
                     ESPEHLVRLLRTQVEPGWPPGEYDRDAQEFFGCRLKEGGASVVGWYVWYAVRREEENQ
                     PSMLVGTGGYFGPPNEGGEVEIGFSIMPSARKDGYATEMARALVSNAFYDIRVHKVIA
                     RTTSDNMASVKVLTKSGFRYIRRDPSGNDVFEILRS"
     gene            3652..4653
                     /locus_tag="PLG98_10805"
     CDS             3652..4653
                     /locus_tag="PLG98_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE33795.1"
                     /translation="MPRSARIDAADAVHHIMVRGIERKKIFESDEDRKHFLHRLGEIL
                     QDTGTTCFAWSLMPNHFHLLCRTGPVPLSTVMRRLLTGYAVWFNRSRRRHGHLFQNRF
                     KSILCQEEAYLLELVRYIHLNPLRAGLVRGLDELDSYDYSGHSTLMGKNKNPWQETEE
                     ALRYFSSRPASARKAYRRFVEAGIAQGRRPDLAGGGLIRSAGGWDSLKQKRREGVYQR
                     SDERILGDSEFVTRVLARSKEQMNRRHVLRAAGMDLDRIAKHVSTITGLAPKDILSAG
                     RQRPIVRARSLFCYWAVRELGVSMSGLANRFGISVVAVSKSVVRGREIAEQEGISLL"
     gene            complement(4648..>5052)
                     /locus_tag="PLG98_10810"
     CDS             complement(4648..>5052)
                     /locus_tag="PLG98_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33796.1"
                     /translation="IKKLKSANLALKGKSEREFEQAVIGHLQSSPTLRKNLITQVGTD
                     EVEKIMQANLFGFCHRPDASIGNDGTAIEIKVISGGQSARDILGQSIAYRMQYRFVII
                     VLVDGTEDRKIVELCKDKKRKKHRDAVNFLTL"
BASE COUNT         1206 a         1262 c         1370 g         1214 t
ORIGIN      
        1 tgccgcagac ggcctgatcg atggccgtta ccggcttgtt ccggaaactc tgcacgtgct
       61 cttttgtgcc gctgtggcag acagcgcagg agagcatggc atgtttggaa ccttccttca
      121 gggacttgat ctggaaatgg cacttgtagc aggcttctcc ggagttgctt gccccgtcgt
      181 tcctgcctgc gctttgcgcc gccgcaatcc cggaaaccat cagaataatg aacatgacga
      241 cgaagacgac ctgcccggca ttttttttat acatatccga cctccttgag tttgccccgg
      301 caatcgatgg gttggtgtgt ttccgtttgc ccgcaactga gcaattatcc tgcgaccaca
      361 gaaagattct catgaaactg agggtcaaag tcaaggggtt tcccctgtaa agcccctata
      421 aatcgtttga caaaccgtat cccataaaac gcccttcgga tatgcaagaa atcctgcaga
      481 cggaatacta caggaaatga ttcgataagc gcaaagacaa gaacgcacgt gcgcggccat
      541 ttcgtcctgt gtgcctaatt gacgcttacc gtcgtgctcc agtaatccat gatcatgatc
      601 ccggggtcat cttccatggt atcacaggtc gccgcaggat attgaatgga tgaggtcatg
      661 aagggccgct cccgcgaagg cgggatgagg ggcacgtcga cgtctttctg gacattgcac
      721 cggaagtggt cctggttgcc gaaaaagaag tcctgaaaag ccttcttgtc ctcatcgctc
      781 acgtcggtca ggtatccggc catatccgcg gcggtgggat cgacaggaat ccaaccgtaa
      841 ttgggcagat agaattcagc ccagaagtgg ccgctcaagt tccccgtgaa gagctgccat
      901 cccccagtcg tgcgcgcggg aatgcccaca gaacggcata aggcgctgaa atacatggac
      961 tgggcgccgc aatcgccgta acggttgcga tggacgtaca cggattccgg ctcgccgcgc
     1021 ggccagagag ccaaatgagg catgtagcta tacgtcaccg tcgccaggac gtagtcatag
     1081 atttttttcg ctgcgaggta agggttggtt tcgcctccga tcacggtttg cgcagttcgg
     1141 cggatctcat tggtcacggc aatatttcca caggaagccg tatatttcaa atagagatca
     1201 cttttgcggt cgtacgcccc cactctatcc ggatcgatct gaaaccattg ctcatagtgg
     1261 tcgaaggtga agcggatcac aatatggagg tcctgcgtca gttcagacag gggaacctcc
     1321 atgtagatca atccgatatc ctggtcgatg gacggagaac ctttcacata ggtcatcggc
     1381 gttatggaaa gcacggaaac attcctctgc gggcctgtaa cgatggcgat gggaaaccac
     1441 agtttcaaga tgccgccggc gggcaatttg ccccggggaa cggcaagcgt acaggtcgcc
     1501 gtgtaggata tcggcctgat gaaaggctgc cagggatcgg cgcccgggcc ggcatccacg
     1561 acctgtttga gaagcttgta gtgatcttcg aagcctttgt acatattcgc gtcctggcga
     1621 aagagatcga cgttccggaa tttgacattg gaaataaaat ccgaaaaaaa gcgcgattcg
     1681 ccgtcgatca tggtgctctc cagtttcccc tgggtgatcc aggcctcccg ctcttccggc
     1741 gtcacctgtg gaaaggcatc ggccagcatc gcccgcgtct cgccgagacg gtaggggtac
     1801 tgagccgtga tcagttggat atcccgcagg ctgttgaggc attccagcgc agcgtcattt
     1861 ttcccttcgt tcgtcagcag cgtccgcgcc tgccggtaaa gacctgtagc cgtggtaaac
     1921 tcgcctcgtg ccttggcctg cctggccagg gcaagcatcc ctgtccccgg agacaccgtc
     1981 agcgaatccc ttccgccgca ggccgtaaca accagcacaa aggccaaaag accgacaacg
     2041 gacatcttca tccaagatgc atgcatggtc gtaccctcgc ttgctttttt caacagacaa
     2101 attgatgact gaatcgataa gtgcttcttt aatggacgcc gattatagga ggcggaatat
     2161 gcgatgtcaa ggcgattctt ggctgaaaaa aacgccactt taaaatctgg caattctgca
     2221 tgaagccaga tcatccaggg ggctcaagca gcgtgaagga aggttaaaat taaaaagtgc
     2281 actacgtgcc tgtgtttcct tgagggcgca tttcccggcc ggagcgtgga tcggtcgatc
     2341 ttgcttttgc cggaaaaacc ctttgccggc cgttagacct tgctgcgttg ggatatttct
     2401 tagccagagt atccggaaca tcaccgtcgg gggatgaatt actttcctgc ttcgtgtcgg
     2461 ggcaatttct tcttccagag gaatgtccag aacgtgtttg taaagaaaga ccagagcgtt
     2521 tagcgcctgc cgctgtgttg aggacgtgcc ctgtccgtcg gtagccagat gagcaaggaa
     2581 ggtcgcgaca ttcttcgccc caagaagctg cggatgggtt ctcccgccga agtattggat
     2641 ataacgaaga attcattgac agtaggtctg ttctgtgcga taggcgcaat ggtgataccg
     2701 aaggacctcc cgaacctggt ccattagcct gagcttcgga ttcgggtgga atttcggttg
     2761 catttgcctg ttcatattaa attttaaaac aatattcaga tggtgctttt agaaacccgc
     2821 caaaggcggg accggcgaca atggggctta caagcaaatt tttccatgtt tgccccttta
     2881 atggcgtaat aagcggaata tggcaagcgg aaaggtaata agtggaaaat ccgggcgtct
     2941 atagggtctg gtatgatgga aaattttcta cttattactc tgttatcaga gaaatacaaa
     3001 atggatgaac taaaaaatgg cactttacgg acagaccgac ttgagttaac agcggcgaca
     3061 tttgaccata ttcacgccga aatggaatcc cctgaacacc tagtacggtt gttgagaacc
     3121 caggttgaac ccggatggcc tccaggggag tatgaccggg atgcacagga gttcttcggc
     3181 tgtcgcctca aagaaggtgg cgcgtcggtt gttgggtggt atgtttggta tgcggtacgc
     3241 cgagaagagg agaatcagcc ttcaatgttg gtgggaacag gcgggtactt cggcccccca
     3301 aatgaaggag gcgaagtaga gattggcttt tccataatgc cgtcggcgcg aaaggatggg
     3361 tatgcgactg aaatggccag agcacttgtc tcgaatgcgt tttacgacat ccgagtgcac
     3421 aaggttattg cccgcacgac ttctgacaat atggcgtcgg tcaaggtgct tacgaaatct
     3481 ggcttccgtt acattcgtcg agatccgtct ggcaacgatg tttttgaaat actgaggagc
     3541 tgataacaac cgcatgaacc cgatcgtgtg taataacata ataacatagg gacgctgtta
     3601 acatttaaac ttattcttga catattgcta aagaatggct aagaggaagg catgccgaga
     3661 agcgcaagaa tagacgccgc tgatgccgtc catcacataa tggttcgggg catcgaacga
     3721 aagaagatct ttgaatcgga tgaggaccgg aagcactttc tccatcgctt gggagaaatc
     3781 cttcaggata ccggcacgac ctgctttgcc tggtcgttga tgcccaacca ctttcatctg
     3841 ctttgccgca ccggcccggt tccgctctca accgtcatga ggcggctctt gaccggctat
     3901 gcggtgtggt tcaaccgcag ccgccgtcgc cacggtcatc tgtttcaaaa ccgctttaaa
     3961 tccattttat gccaggaaga ggcctacctt ctggaactcg tgcgctatat ccatctcaat
     4021 ccgctgcgcg ccggattggt gcgggggctg gatgagcttg attcctatga ttattccggc
     4081 cacagcaccc tgatgggcaa aaacaaaaat ccgtggcagg agacggaaga agctctcaga
     4141 tacttcagct ccaggccggc ttcggcccgg aaagcctatc gcaggtttgt tgaagccggc
     4201 atcgcgcagg ggagacgccc cgacctggcg ggcggcggtc tgatacgaag cgccggaggg
     4261 tgggacagct taaaacaaaa aagacgtgaa ggcgtctatc agcgcagtga tgagcggatc
     4321 ctgggggaca gcgagttcgt caccagagta ttggcgcggt cgaaggaaca gatgaatcgg
     4381 cgccatgtcc tgcgcgcggc ggggatggat ttggacagaa ttgccaaaca tgtttcaaca
     4441 attacgggac tggcgccgaa agacattctg tcggccggca gacaacggcc gattgtgagg
     4501 gcgcggagtc ttttttgtta ctgggcggtt cgggagttgg gcgtttcgat gtccggtctg
     4561 gcgaaccgat tcggaatatc cgttgtcgcc gtaagcaaat cggttgtccg cggccgcgag
     4621 attgcagagc aggaaggaat ttccctgcta taaagttaag aagttaacag cgtccctatg
     4681 tttcttcctt tttttatcct tgcatagctc aactatcttt ctgtcttcgg tgccgtccac
     4741 taatacgatt attacaaacc gatattgcat gcggtaggca atggactgcc ccaaaatatc
     4801 tctcgctgat tggccgcctg aaataacttt aatttcaatt gcagtaccat catttcctat
     4861 ggaggcatca ggcctatgac aaaatccaaa aagattagct tgcataattt tttcgacttc
     4921 gtctgtacct acttgcgtta ttaaattttt tcgaagtgtt ggagaagatt gcagatgacc
     4981 tattacagcc tgttcaaact cccgttccga ttttccctta agcgcaagat ttgcactttt
     5041 taattttttt at
//
LOCUS       DAOSME010000111         5045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_18099_length_5045_cov_16.173146, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000111 DAOSME010000000
VERSION     DAOSME010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18099_length_5045_cov_16.173146"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PLG98_10815"
     CDS             complement(<1..1252)
                     /locus_tag="PLG98_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOE33797.1"
                     /translation="MKNINPQAVSIKKTDLSYFPLNDLRQASENGLLYDKYSPNADED
                     DAKLYWSIKANGIREPLHISADGYILSGHRRYGAASWLGLAVVPCIVADDIIWGDLSA
                     DDRLGVLALYNKQRSKSQAELLREAMQEISPEEAYQKLLQYRIGQRQIDIDENVELGE
                     CKRRARITTQAFLQAAQRAIESEKEYWPLTVRRVHYLLLNAPPLRHDKKPGSTYRNDL
                     RSYKALTNLLLRARLAGEIPQEAIEDELRPIRVLKTYQNAADFIKDEATDFLRGYARD
                     LLRGQKNHIEIMVEKNAIRKHVEAVADEYCIPCTTGRGYSSLTPRLKMARRFLRSGKD
                     QLILLILSDFDPDGEEIAASFPRSLRDDFGLSNIKSFKVALSGADVKKYDLPSDMEAK
                     RSSPNYRKFVERHGTHVAELDAAPV"
     gene            complement(1261..1536)
                     /locus_tag="PLG98_10820"
     CDS             complement(1261..1536)
                     /locus_tag="PLG98_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33798.1"
                     /translation="MKRKTLLLILIAIVLLLPIPIPTPIPLPALIPIPIPTTPMMKPM
                     LEKDYSKSVVIKNDRFFPLDVIQQMLPTTLPGGDHLPGRNPYQYNGN"
     gene            complement(2623..3483)
                     /locus_tag="PLG98_10825"
     CDS             complement(2623..3483)
                     /locus_tag="PLG98_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33799.1"
                     /translation="MDFNKRLAETMIKAVNNPDYINTEIFKWKHEKEMSQKLSDDKIQ
                     FLNNLIKNIEEILKQNPRKISSYPHIREADLLDINGRPYGDWCNFRRQRREAGIFFPL
                     AHYDFRDPSGFLILRDDCSDLLDLLQSGRFNSINLISGILTNRCDFIMARFIYLNAYT
                     GITPNSAPDYLKAGFSTFLASKRGKSGGEKPKMENAIYKALEQFIKEDPDLIQASNNS
                     LARKFAKWLLSDDRYIEHATREWYIFYVEDSKLIFAEPDKTTKKSKITSLKLGTIRTE
                     YIPKIKNTEK"
     gene            complement(3584..4816)
                     /locus_tag="PLG98_10830"
     CDS             complement(3584..4816)
                     /locus_tag="PLG98_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HOE33800.1"
                     /translation="MPKRVKPLTDTKVRTAKPQHKEYKLFDGEGLFLLITPTGGKLWN
                     FKYRFDKKEKKLALGSYPEISLLDARRKRDDARRLLANDVDPGAVRKAQKQAKVEETE
                     TFEVIAREWHEKFKQKWVDGYADKLMRRLERNVFPWIGSKPIKDLKAPELLAFLRRIE
                     SRGALELAHRIRNVCGQIFRYAVATGRAERDPSSDLRGALTPVKTIHMAAITDPVKVG
                     ELLRAIDAYQGSFVVQCALKFAPLTFVRPGELRHAEWSEIDFEKAEWNIPGHKMKMDQ
                     PHLVPLSSQAIEILTALKPLTGEGRYLFPSERSSDRSMSNNAILAALRRMGYTKEEMS
                     GHGFRAMARTILDEVLQVRPDLIEHQLAHAVRDPLGRAYNRTQHLNERRKMMQTWADY
                     LDGLKEGAKVLPFKAKEA"
BASE COUNT         1223 a         1183 c         1096 g         1543 t
ORIGIN      
        1 caacgggtgc agcatccaat tcagcaacat gagttccatg ccgttcaaca aattttcgat
       61 agttcggaga ggatcttttt gcttccatat ctgacggcag gtcgtatttt tttacgtccg
      121 cgccggatag tgcaaccttg aagctcttga tattcgataa cccgaagtca tcccggagtg
      181 aacgtggaaa gctcgctgct atctcttcac cgtccgggtc aaagtctgac aggatcaaaa
      241 gaataagttg atccttgccg gatcgtaaga agcgtctggc cattttcagc cgtggagtta
      301 agctgctata tccgcgcccg gtagtgcaag gaatacagta ttcgtcagcc actgcttcaa
      361 cgtgctttct gattgcgttt ttctctacca tgatttcgat atgatttttt tgaccccgca
      421 acaggtcgcg ggcataaccc ctaagaaaat ccgttgcctc atccttgatg aagtccgccg
      481 cgttctgata tgttttaaga acgcggatag gtcttaactc atcctcaatc gcttcttgtg
      541 ggatttcacc ggccagccgc gcccggagta aaaggtttgt caatgccttg tagctgcgaa
      601 gatcatttcg atatgtcgat ccgggttttt tgtcgtgccg caaaggtgga gcgttcagca
      661 acaggtaatg gacacgccgc accgtcaacg gccagtattc cttttctgat tctatggccc
      721 gctgcgccgc ttgcaagaaa gcctgcgtcg tgatccgcgc ccgtcgcttg cattcaccca
      781 gttcaacgtt ttcatcaatg tcaatttgcc gctgtccgat ccgatactga agcagttttt
      841 gatatgcctc ttcaggtgaa atttcctgca tggcctcgcg gagcaactct gcctgtgatt
      901 tgctccgttg cttgttataa agggccagca cacccagacg gtcatcggcg ctaaggtcgc
      961 cccatataat gtcatcggca acaatgcagg gcacaactgc cagccctaac catgaagcag
     1021 caccataacg tctatgcccg gataaaatat agccgtcggc ggagatatga agcggctccc
     1081 gaatgccgtt agccttgatg ctccaataca gtttcgcatc atcttcgtct gcgttcggcg
     1141 aatatttatc gtatagcaac ccgttttcag aagcctgccg aagatcgttc aatggaaaat
     1201 aggacaagtc cgtttttttt atggagactg cctgcggatt tatgtttttc atgctcgatc
     1261 ctaatttccg ttatattgat aggggtttct tcccggcagg tgatccccac cgggaagagt
     1321 tgttggaagc atttgctgaa tgacatccaa cggaaaaaat cggtcgtttt ttatgacaac
     1381 gcttttgctg tagtcctttt ccagcatcgg tttcatcata ggggtagttg gtatgggtat
     1441 tggtattagt gctggtaatg gtataggtgt aggtataggt ataggtaata gtaaaactat
     1501 tgctatgagt atgagtaaaa gagtttttct tttcattcta ttctcttctt aatgtaatcc
     1561 acaacggagt aatggaaaca ttcgggtttc tccgttccat cgggaaattt tggtttgaca
     1621 gtcctgacaa cagcctcgaa aacaccgttc ataaatattc ttgatgacat tcggtcgttc
     1681 cgtgccgggt gaatattcct gtttgcaatg acccattgct cataatattt cgacccgcta
     1741 ggcacttgct tgaatggttt gcccgtccgc gtgttgatta ggttcatggc cataaaaagt
     1801 tccgtcccgc tgtattgccc gaagtttatg atcttgaaag tcaaaaacat tttgaatgac
     1861 cgaaaatgcg aaacggcttt ttcagcctta acgcaacaaa cgcggtaaac cccttcggga
     1921 ataagtggcc gcgcttcaga cgccacaaat ttttcatcat tttgttcatc ctgaattgaa
     1981 acgttttttt taagctcata tgccgtgttt gtcattgccc cttgcccctc atatgccttt
     2041 ttggttttat gtccctgaaa gcctcatttt aacgtgctcc agaaggaaag aggggcacta
     2101 ttggacaagg ggaagcttcc gggtttgtcg ttcctcgcgg aatggcatga atgggtaaca
     2161 ggggcacttc gggattttgc aatccatcaa gccatctgcg aaataacaac aacactcaaa
     2221 acattttgcc gcaatcgctt ttcggggtgt gatccgcttg ccctctaaat gctcgatcaa
     2281 ttctcgcttg ccccgtgctc gtttgccata cttgcgaatt tcagctatga gttcttcatt
     2341 cattgtggtc atacctcacc atttatcagg cgacgcacat cttctgattt ccagcaagtt
     2401 gtccgttcac ccaatttgac cggtttagga tatttgcccg ttcgcacccc ctgccaccat
     2461 gaagaacggc ttacaggtat gaatttcaat acctgcttaa tcctcaatag gcctgtctgt
     2521 ggaagttctt ggagctgtgg aattttggaa tctcttgaca tttgatgccc tccttttttg
     2581 atttttggag agcataaaga aggggctgtg cgggaaagcg gcttacttct cagtattttt
     2641 tattttggga atatattctg ttcggattgt accaagtttt agggaagtaa tcttactttt
     2701 tttggtggtc ttatctggtt cagcaaaaat taatttactg tcttcaacat aaaaaatata
     2761 ccactcacga gttgcatgtt cgatatatct gtcatcagat aaaagccact ttgcaaattt
     2821 acgcgcaagg gaattgtttg acgcctgaat taaatcaggg tcttctttta taaattgttc
     2881 cagtgcttta taaatggcat tctccatctt gggtttctca ccgcctgatt taccgcgttt
     2941 cgatgctaaa aaggttgaaa accctgcttt aagataatct ggcgcactat tcggggtaat
     3001 gcctgtgtaa gcattcaaat aaataaacct tgccattata aagtcgcaac gattcgtaag
     3061 aatgccggat attaaattta tagaattaaa ccttcctgat tgtaatagat cgaggagatc
     3121 ggaacaatcg tctcgaagga tgagaaagcc agatggatct ctgaagtcat aatgagccag
     3181 agggaaaaat attcctgcct cccgccgttg tcttcggaaa ttacaccaat cgccataagg
     3241 cctgccgtta atatcgagca aatcagcctc ccgaatatgg gggtatgagc ttatttttct
     3301 tggattctgt tttaagattt cttcaatatt tttaatgaga ttgtttaaaa attgaatttt
     3361 atcatcactt aacttttggg acatttcttt ttcgtgcttc cacttgaata tctcggtatt
     3421 gatgtaatct ggattattta cggccttaat catcgtttcg gccaggcgct tgttaaaatc
     3481 cattttgctt tccctctcgc aagctcccca taaagttata tcagggaagc ggaaaagggg
     3541 ttcttcccgc ttatcggccc gtcggcctat ccctgaatta tgtttatgct tctttggcct
     3601 taaacggcaa aacctttgcg ccttccttca atccgtccag ataatccgcc catgtctgca
     3661 tcatttttcg gcgttcgttt aaatgctgtg tccggttata agcgcggccc aatgggtcac
     3721 gaacagcatg ggcaagctga tgctcaatca aatccggtct gacctgcaag acttcatcaa
     3781 ggatcgttcg cgccattgcc cggaatccgt gacctgacat ttcctctttc gtatatccca
     3841 tgcggcgaag tgcggccaat attgcattat ttgacataga acggtctgaa gatcgttcag
     3901 aggggaaaag atagcggcct tcacctgtta atggtttgag cgccgttaat atttcaatag
     3961 cctgactcga caatggcaca aggtgtggtt gatccatctt catcttgtgc cccggaatgt
     4021 tccattcagc cttttcaaaa tctatttctg accattcagc atgacgcaat tcccccggac
     4081 gaacaaatgt gaggggcgca aactttagcg cacactgtac aacgaagctt ccttgataag
     4141 cgtcaatcgc tcttaacagt tccccgactt ttacaggatc ggttatagct gccatgtgaa
     4201 tggttttaac cggtgtcaat gcaccgcgca aatctgagga tgggtcacgt tcagctcgac
     4261 cggttgccac tgcatagcgg aatatctgac cgcaaacatt tcttatccgg tgggcaagtt
     4321 ccaatgcacc ccttgattct atacgcctta agaatgccag caattcaggt gcttttaaat
     4381 ctttaatcgg tttagaacca atccaaggaa aaacattccg ttctaaacgc cgcatcagtt
     4441 tgtctgcata tccatctacc catttttgtt taaacttttc gtgccattct ctggctatga
     4501 cctcaaaggt ttctgtttct tcaacttttg cttgtttttg ggctttacga accgcaccgg
     4561 ggtcaacgtc gtttgccagc aaccgccggg catcatctcg cttacgtctg gcatcaagca
     4621 aacttatttc cgggtaactg ccaagggcaa gtttcttttc ttttttatcg aagcggtatt
     4681 taaaattcca caacttaccg ccggtgggag taataagcag aaaaagccct tcaccgtcga
     4741 aaagtttata ttccttgtgc tgtggttttg ctgttctgac ttttgtgtct gtaagcggct
     4801 taactctttt aggcataagc gtttcctcgt ttttgggggc ttccaattat ttttgggggc
     4861 tttttgcgct aaaacagccc ccaaaactac cggatttcgg gaaacaatat aacacgtggt
     4921 tggatgaatg gcaataaaaa acccgctatt tctaacgggt tttgtactaa tccagacttt
     4981 actggattat gtattggctc cccagcgcgg actcgaacca cggacatgct ggttaacagc
     5041 cagcc
//
LOCUS       DAOSME010000112         4949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_18550_length_4949_cov_21.352268, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000112 DAOSME010000000
VERSION     DAOSME010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18550_length_4949_cov_21.352268"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLG98_10835"
     CDS             complement(<1..538)
                     /locus_tag="PLG98_10835"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOE33801.1"
                     /translation="MSANGLVQIAIYFLALIALAVPLGNYMARVYAGEYVFLSSIAGP
                     LERFIYRCCRIDEKQEMSWQNYAAGLMIFNLIGFLALYLLQRCQDLLPFNPQGFPGVP
                     PALAFNTAVSFVTNTNWQAYGGETTLSLLTQAAGLTVQNFASTASGMAALIAFTRGLK
                     RTLANTIGNVWVDLTRSLI"
     gene            complement(535..627)
                     /gene="kdpF"
                     /locus_tag="PLG98_10840"
     CDS             complement(535..627)
                     /gene="kdpF"
                     /locus_tag="PLG98_10840"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HOE33802.1"
                     /translation="MTLFYLAGLIIVIGLAVYLFLALLKPEWFQ"
     gene            complement(1004..2815)
                     /locus_tag="PLG98_10845"
     CDS             complement(1004..2815)
                     /locus_tag="PLG98_10845"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOE33803.1"
                     /translation="MYIRRTTHADKKNKKEYHTFKLIESVRTERGPRQRMLLNLGTDF
                     SLPEDRWKDLANRIEEIITGQDALFAYPADIENLAVRYARKVIDYQGEPGGGGIKSTD
                     PDYHRVDIDTIDNERSRSAGAEHVIYETIKSLGINDLLMALGFNKPALDAAIGVIAAR
                     LIAPSSERATHLWLQEMSAMDELLGADFTDLSQDRVYKTSDLLLRHKKQIEDHLCAKE
                     CRLFNLEEKIILYDLTNTFFEGSGRYNGKAHFGPSKEKRSDCPLVTLGMVLDADGFPK
                     RTEVFAGNVSEPGTLEGMIKTLAFADMFTRPLIVMDAGIATQSNILWLKEKGFDYIVV
                     SRKKRIDLPLETEMITVREDNRRLIRAAITKGPDDDEVTLYCHSTDKEIKERGIKNLF
                     ESRFEEKLEQIRNALSKKHGTKVYEKVVEKIGRLKERYKRISSRYEIAVTKDENTNWA
                     TTIAWERKEQEKPAGVYCLRSNRIDFKEQELFNIFAMLTDIEDAFRSMKSELGMRPVY
                     HQNENRTDGHLFITVLAYHILHSIRVHLRSQGVNDCWTTVRKRLSTHVRITTTMKRDD
                     GKSIHIRKSTRPEAYHKIIYDALQLSPHPGITIKTIL"
     gene            complement(3217..4173)
                     /locus_tag="PLG98_10850"
     CDS             complement(3217..4173)
                     /locus_tag="PLG98_10850"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOE33804.1"
                     /translation="MDTRRYFIKKIAIVAAALCLPVSLPCENLAAYPDLKTRNPKRVL
                     VLWYSQTGQTRRYARLIGCILEGKGLTVDARDMQEFDKKLLTNYDLIVFGTPVFYYDT
                     PSNVSEWLKTIPSIEGTPVAAFVSFGGPEGNQYNSSSHILKLLAGRGGVPVGRDTFRN
                     VASYPTPKWDNPKQISGQHLPDAGTYDQVRRFTAEVMKRITRGETISVGYEVALREGL
                     SLLPLVWMNKKAISKHTVNAIKCIGCQTCVRKCPTKAINPSMQTVDRDKCLACFGCLN
                     NCPADAVVMEYRGERLYGFPEYLKRNKITIMEPPEFKTCKLS"
     gene            complement(4204..4521)
                     /locus_tag="PLG98_10855"
     CDS             complement(4204..4521)
                     /locus_tag="PLG98_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE33805.1"
                     /translation="MKGRKLSDKEIELHEEKRDVWREVLDGIDEIKAGGGKRFVVAPK
                     TEVAKARIKTGLSQSQFAALMGVSKRTLEQWEQGRREPSGAAKTLIKIADLRPDVLKE
                     MTV"
     gene            complement(4523..4828)
                     /locus_tag="PLG98_10860"
     CDS             complement(4523..4828)
                     /locus_tag="PLG98_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33806.1"
                     /translation="MFTFIESSIFSRELANYLDDDEYGDLQQHLINYPESGDVIPGSG
                     GVRKLRWQRRGMGKRGGVRIIYYVMAKQDEIWMLTLYAKSVLDNIPAHIVKAMKERF"
BASE COUNT         1228 a         1225 c         1144 g         1352 t
ORIGIN      
        1 aaataagcga acgggtcaga tccacccata cattgccgat ggtattggcg agggtacgtt
       61 tcagccctct ggtaaaggcg atcaacgccg ccattcccga tgccgtggag gcaaagttct
      121 gaacggtcaa tcctgccgcc tgggttaaaa ggctcaaggt cgtttccccg ccgtaggcct
      181 gccagttggt attggtcaca aagcttaccg cggtgttgaa ggcaagggca gggggaacgc
      241 ccgggaatcc ttggggattg aaaggaagaa gatcctggca ccgctggagc agatagaggg
      301 caaggaagcc gatcaggttg aagatcatca agcctgccgc atagttttgc caggacattt
      361 cctgcttctc gtcaatccga caacagcgat agatgaacct ttccaggggg ccggcgatgc
      421 tgctcaggaa aacgtattcc cctgcataga ccctggccat gtagtttccc aacggtacgg
      481 ccagggcaat aagggccagg aagtatatgg caatctgaac aagtccattg gcgctcattg
      541 gaaccactcc ggtttcagta aagcaaggaa aagataaacg gcaagaccga tgacaataat
      601 caatccggca agatagaata gcgtcatttc cgcccctccc taaagatcgt cgaatttcct
      661 gatgaagaac caggttattc cggaaaatat gccgatcagg catagaaata ttaaatccat
      721 gagcttgttc ctctcctttt ggatttgata ttaacaatca agatataagg atgctgttaa
      781 aaaagggggt gaaaatataa agattccgtt aagaaaacgt ttttctcccg aagggaaatg
      841 accaggaccg gatgggggcg acaagccgcc gtcccaggga gggacgccgc gggtatggga
      901 aaagataagg ttatcccaag ttcatggttg tgaaaataat aacgagtgat atcgggcgca
      961 tgtgtggact tggtttcatg tttaggcact acggattttg atcttacaag atagtcttga
     1021 tcgttatacc tgggtgcggg gacagttgca gggcatcata gattatctta tggtatgctt
     1081 ctggacgtgt ggattttcgg atgtggatgc ttttgccgtc atcccgtttc atggtggtag
     1141 tgatgcgcac atgagtggac aaccttttcc ggacagtggt ccaacagtca ttgactccct
     1201 ggcttctcag gtggacgcga atactgtgca ggatatgata agccaagacc gttatgaaga
     1261 gatggccgtc tgtccggttt tcattttgat gatataccgg gcgcatcccc agctctgatt
     1321 tcatgctccg gaaggcgtct tcaatgtccg taagcatggc aaagatgtta aataattcct
     1381 gctctttgaa atcaatacgg ttggatctca ggcagtaaac accggcaggt ttctcttgtt
     1441 cctttcgttc ccaggcgatg gtcgttgccc agttcgtgtt ttcatccttt gtcaccgcaa
     1501 tctcataacg agaagaaatc cgtttgtagc gttccttgag ccgaccgatc ttttcgacta
     1561 ccttttcata aaccttggtt ccgtgtttct tggatagggc gttacgtatc tgttccagct
     1621 tttcctcaaa acggctttca aaaagatttt tgattcccct ttccttgatt tccttgtccg
     1681 tcgaatggca atagagggtg acctcatcat cgtcgggtcc ctttgttatg gcggcccgga
     1741 tcagtctccg gttgtcctcc cgaacggtaa tcatctccgt ctccagggga aggtcgatcc
     1801 tctttttgcg ggaaacaaca atgtagtcaa aacccttttc tttcagccaa agaatgttgc
     1861 tctgggtggc aatgcccgca tccatgacga tcagcggcct tgtgaacata tccgcaaaag
     1921 caagggtttt gatcatcccc tccagcgtac ccggctcact tacattgccc gcaaagacct
     1981 ccgtacgttt gggaaaaccg tcggcatcga gaaccattcc cagggtaacc agaggacaat
     2041 cacttcgttt ttcctttgaa gggccaaaat gggccttgcc gttataccgg ccggagccct
     2101 caaaaaaggt gttggtcaga tcatagagga tgattttctc ttccagattg aacagcctgc
     2161 attcctttgc gcacagatga tcttcaatct gtttcttgtg acgaagcagc aaatcggagg
     2221 tcttatacac ccgatcctgg gaaagatccg tgaaatcggc gcctaagagt tcatccatgg
     2281 cgctcatctc ctgaagccat agatgggtcg cccgctccga agaaggggca atcaaacggg
     2341 ccgcgatcac cccgatagcc gcatcaaggg ccggcttatt gaatccgagg gccatcagca
     2401 gatcgttaat gcccaaagat ttgatcgtct catatatgac atgctccgcc ccggcggatc
     2461 gggaacgctc gttatctatc gtgtcgatat ccacccggtg gtaatcggga tcagtagatt
     2521 ttatcccccc gccacccggt tccccttgat agtcaatgac cttacgggcg tagcggacgg
     2581 caaggttttc aatatccgcc ggatatgcaa acagggcatc ctggccggtg atgatctctt
     2641 ctatccgatt ggccaagtcc ttccagcgat cttcgggcaa ggagaaatcc gttcccaggt
     2701 tgagaagcat ccgctgcctg ggtccccgct cggtgcgcac ggactcgatg agcttgaagg
     2761 tgtggtattc ctttttattc tttttgtcgg catgcgtggt tcttctgatg tacatggaaa
     2821 gtggttaaca tgaaattcac ttcttgtcaa ggcccatcaa taagattttg gcactacaaa
     2881 tgactttttg acgggccata acttataagt atccgatatt attagaagtg actcttgaaa
     2941 aatgaccccc aaaagccatt ttttgaggaa aaaccatgaa cttgggttaa tatctttcct
     3001 cagcctgctt tggaatgtcc gaaccttgcc ctttttcaca tttcacctcc tgcaggaaat
     3061 ctccgtatcc attcttccat gcgtttttca gccaagtcga tgtcctgctt tttcaatgac
     3121 ggcgttttct tggataatgc aatttaaaag taaggaactg cacaggacct tgtgtctaaa
     3181 agtaaacgac gtttcaaaat caaaaagaca gtggaatcat gataacttgc atgttttaaa
     3241 ttccggcggt tccatgatgg ttattttgtt gcgcttcaga tattccggga aaccatatag
     3301 acgctcgcct ctgtactcca taactacagc atcggcagga caattattca aacaaccaaa
     3361 gcaggccaag catttatccc gatcaacggt ttgcatggag ggattgatgg ccttagttgg
     3421 gcattttctc acgcaagttt ggcagccaat acattttatt gcattaaccg tgtgcttgct
     3481 gattgccttc ttgttcatcc agaccaaggg cagcaaactc aatccttccc tcaacgccac
     3541 ttcatatccg acagaaattg tttcgccgcg agtaattctt ttcataactt ctgctgtaaa
     3601 gcgtctcacc tgatcatatg ttcccgcatc gggcagatgc tgcccggaaa tctgtttagg
     3661 gttatcccat ttgggcgttg gatacgaagc aacattccta aaggtatccc ttccgacagg
     3721 aactccgcct ctgcctgcca aaagtttgag aatgtgactg gaagagttat actgattgcc
     3781 ttccgggcca ccgaaggaaa caaaggctgc aaccggcgtt ccttcgatcg aaggtatcgt
     3841 tttaagccac tcgctgacat tggagggggt atcataataa aataccggtg taccgaacac
     3901 gatgagatca taattagtta gcaatttttt atcaaattcc tgcatatcac gtgcatctac
     3961 cgtaagacct ttcccttcca ggatgcagcc gataagcctg gcatacctgc gcgtctgacc
     4021 tgtctgacta taccataaga ccagcaccct tttcggattg cgtgttttca aatcaggata
     4081 agctgccaag ttttcacatg gcagagaaac cgggaggcac agcgcagcgg ccacaatagc
     4141 aattttctta atgaagtatc gtcgtgtatc catatttgag tcgagttgat cagtttctct
     4201 ggatcaaacg gtcatttctt tcaacacgtc agggcgtaga tccgctattt tgattaatgt
     4261 ctttgccgca ccggacggtt ctctcctccc ctgttcccat tgttccaacg ttcgtttcga
     4321 cacgcccatc aatgcagcaa actggctttg ggaaagccct gtctttatgc gcgccttggc
     4381 aacttctgtt ttcggcgcga cgacaaatct tttcccgcct ccggccttaa tttcgtctat
     4441 gccgtccaat acttcccgcc atacgtcccg tttctcctca tggagttcaa tctccttgtc
     4501 tgacaactta cgccctttca tattaaaacc tctctttcat agccttgaca atgtgcgccg
     4561 gaatattgtc aaggaccgac tttgcataaa gggtcaacat ccatatttca tcctgttttg
     4621 ccataacgta ataaataatg cgaactcctc cccgtttgcc catgccccgg cgctgccatc
     4681 gaagcttacg aaccccaccg gaacccggaa taacatctcc tgattctgga tagttgatca
     4741 agtgttgttg caggtcgcca tattcgtcat cgtcaagata attcgcgagt tctcgtgaga
     4801 atatcgatga ttcaataaat gtaaacatag ctcccatgta cgcctaaagc gtataggtgt
     4861 caagatttat ctttttgaaa ctctaaaagg ctttgtaaag ttatccccat agtcaagaca
     4921 aaaaattggg ttgtatttgg tgcatagtc
//
LOCUS       DAOSME010000113         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_20034_length_4661_cov_25.375163, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000113 DAOSME010000000
VERSION     DAOSME010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20034_length_4661_cov_25.375163"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLG98_10865"
     CDS             complement(<1..1053)
                     /locus_tag="PLG98_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33807.1"
                     /translation="MTETRIDYSLGEYQAAVKSALDKMCRDNVIERIRSKDYTLWKFR
                     PDEIVNRLGWIDAPAETLAKINDIRSVVDALQKDKISDIVLIGMGGSSLAAEVFGNIF
                     GSKPGYPRLHVVDTTDPVFISEVTQHLNLEKTLFLVSSKSGSTLEITSLFKYFYNLCL
                     KKFGGEAGRRFIFITDEGSPLIKTAESISARHIFLNNPDIGGRYSALSLPGIVPAALI
                     GIAVETLLQNVSSNLEALMSAGASLGAALGMLANQGRNKMTFVLPPHWKHFGDWLEQL
                     IAESTGKEGRGILPVLNETLLDASGYGKDRIFVIFQNKNEEEPSQMETLKKDGHPVIT
                     VKINDDYELGQQMYIWE"
     gene            complement(1195..3252)
                     /gene="tkt"
                     /locus_tag="PLG98_10870"
     CDS             complement(1195..3252)
                     /gene="tkt"
                     /locus_tag="PLG98_10870"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOE33808.1"
                     /translation="MSDKNELEKQCIDAIRFLAADAIEKAKSGHPGMPLGAAAAAFTL
                     WTKHLKHNPKNPQWPDRDRFVLSAGHASMLLYALLHLTGYDLTLDDIKTFRQWGSRTP
                     GHPEYKHVPGVEVTTGPLGQGLANAVGMAVAEAHLAARFNGEDQKIVDHFTYVMAGDG
                     DMMEGVAGEACALAGHLRLGKLIVLYDDNKVTLAGSAALSSTENIEQRFKAYGWQVLK
                     VADGNDVAAIDKAIKKAKREAEKPSLICIASTIGYGAPCKQGSCDAHGSPLGADELQK
                     AKEALCWLADSPFSVPENVYTFFRKAVSRGKKDEKKWQESFNNFRQNEPALAAEFDRI
                     MKGDLSDGWESALSEFPNGSKDVATRKAGEIVMQSIAGKIPELAGGSADLNPSCFTWL
                     KGMGDFQSPKLSKEGLHGMVGGAWDYSGRNIHFGVREHAMGSIAVGMALHGGIIPYTA
                     TFLTFADYMRPPMRLAAMMGLRAVFVFTHDSIGVGEDGPTHQPVEQIMNLRQVPNMTV
                     IRPADANETLEAWKIAITNIKGPTTLIFSRQNLPVLDRSVYASASGTKQGGYVLWESA
                     SDPDVILIATGSEVSLTLTAARKLAEAGTKTRVVSMPSWELFDRQPRDYRDKVLPPQI
                     SARIAVEAGIKLGWEHYVGLNGKIIGMETFGASAPGPVLYEKFGFTVEKIIEAARDLL
                     HRS"
     gene            3459..>4661
                     /locus_tag="PLG98_10875"
     CDS             3459..>4661
                     /locus_tag="PLG98_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOE33809.1"
                     /translation="MTISAFEPATLAGIKLKNRIIRSATYEGMADEKGFPTEKLKKLY
                     INLAKGDVGAIITGYAATQADGKVSNAMAMVDHDDKINVFKEITDAVHEYGTPVIMQI
                     AHCGRQTRSRITGFPTVAPSPVRNMYFMEDKPKALSEEGINEIIDNFVAAIVRAKKAG
                     FDGVQLHLAHGYLLSDFLSSHANRRKDRWGGSTENKYRIVGEIFKRARGKVGDYPILV
                     KMNAYDGRRKGMRIEEAVVIARMLENSGCAAIEVSCGCVEDGLFTIRGEKLPVEAVVE
                     YNFNFKNYPAFAKKTIVWFAKTFLKTPKPLLKYNLDAAMQIKKAVNIPVIVVGGINNV
                     EDVEDIIGNDKADFVSMSRPFIIEPDIVSKFKNRTQTTSKCIMCNFCVITIEKEPLKC
                     RYGKLPKTK"
BASE COUNT         1167 a         1180 c         1184 g         1130 t
ORIGIN      
        1 ttcccagatg tacatttgtt gacccagctc ataatcgtcg ttaattttaa cggtaatgac
       61 gggatgaccg tcctttttca gagtttccat ttgagagggc tcttcttcat ttttattttg
      121 gaaaataaca aaaatccggt ctttcccata tcctgaagca tccaaaagcg tttcgttcag
      181 cacagggaga attcccctgc cttccttgcc tgtgctttcc gcaatcagtt gttccagcca
      241 gtcgccaaaa tgtttccagt gcggcggcag gacaaacgtc attttgttgc gaccctggtt
      301 ggccagcatt cctaaagcgg cgcccagaga agcgccggcg ctcatgagcg cttccaaatt
      361 ggatgaaacg ttttgcagca gcgtttccac agcaatgccg atcaatgccg ccggaacgat
      421 tcccggcagc gacagcgccg agtaacgtcc tccgatatcg ggattgttga ggaaaatatg
      481 acgggcggag atactttcgg ctgttttgat cagcggactt ccctcatcgg tgataaaaat
      541 gaaccggcgt cccgcttcgc cgccgaattt tttcagacac aggttataaa aatatttgaa
      601 gagcgatgtt atctccaggg ttgaacctga ttttgatgaa acaagaaata atgttttttc
      661 aagatttaaa tgttgtgtaa cttcagaaat aaaaacggga tccgtcgtat cgacgacatg
      721 caaacgggga tagcccggtt tgctgccgaa gatgttgccg aaaacttctg cggccagcga
      781 tgacccgccc atgccgatta aaacgatatc gctgatttta tctttttgca gggcatcgac
      841 aacgctgcgg atgtcattta tttttgccag cgtttcagcc ggagcgtcaa tccatcccag
      901 gcgattgacg atttcatcgg gccggaattt ccagagggta taatcttttg accggatgcg
      961 ttcaatgacg ttgtcacggc acattttatc caaggcgctt ttaacggcgg cttgatactc
     1021 tcccagagaa taatcaatgc gtgtttcagt catttaggga aactccaatg taatgagact
     1081 cgctaactac acgaagcgtt agttgtagag acgctcgcta aaaaaagcgt agcgcagttt
     1141 gaagcgttag cggaactatc caatcccgat ttatcgggat tggaaactat cccgtcagga
     1201 gcgatgaagc aagtctcttg ccgcttcaat aattttttca acggtaaagc cgaatttttc
     1261 gtaaagaacc ggtccgggtg cggaagcgcc gaatgtctcc atgccgatga tttttccgtt
     1321 caaaccgaca tagtgttccc accccagctt gatgcctgct tccacggcaa tgcgggcgga
     1381 aatctgcggc ggaagaacct tatcgcgata gtcgcgcggc tgacggtcga atagttccca
     1441 actgggcatg gaaacaactc tggttttcgt gccggcttcc gcaagcttgc gggctgcggt
     1501 cagggttagt gaaacttccg aaccggtggc aatcagaatc acgtcgggat cggacgcgga
     1561 ttcccacaga acataaccgc cctgtttggt gccggacgcg gatgcgtaaa cgcttctgtc
     1621 cagcacggga aggttctggc ggctgaagat cagtgtcgtc ggccctttta tattggtaat
     1681 ggcgattttc caggcctcca gtgtttcatt ggcgtccgcc ggacggatta ccgtcatgtt
     1741 gggtacctga cgcagattca taatttgttc gacaggctgg tgtgtcgggc cgtcttctcc
     1801 gacgccgatg ctgtcgtgcg taaagacgaa tacagcgcgc aggcccatca tcgccgccag
     1861 gcgcatgggc ggacgcatgt aatcggcaaa ggtcagaaag gttgccgtgt agggaatgat
     1921 gccgccgtgc aacgccatgc cgacggcaat cgagcccatc gcatgttcgc gtacgccgaa
     1981 atgaatatta cgtccggagt aatcccaggc gccgccgacc atgccgtgca gcccttcttt
     2041 ggataattta ggactctgaa aatcgcccat gcctttgagc caggtaaaac aggacggatt
     2101 cagatcggcg gagccgcccg ccagttcggg aattttgcct gcgatggact gcatcacgat
     2161 ttcgcccgct ttgcgggtgg ccacgtcttt tgatccgttg gggaattcgc tcaaagccga
     2221 ttcccagcca tcggacaggt cgcctttcat gatgcggtca aattctgctg ccaaagcagg
     2281 ttcattttgt ctgaagttgt taaacgattc ctgccatttt ttttcgtctt ttttgccgcg
     2341 cgacacagct ttgcggaaaa acgtataaac gttttcggga acagaaaaag gagaatcggc
     2401 cagccagcag agagcctctt tggccttctg cagctcgtcg gcgcccagcg gtgagccgtg
     2461 cgcatcgcag cttccctgtt tgcagggtgc gccgtaaccg atcgtggatg caatgcaaat
     2521 caatgaaggc ttttcagctt cgcgcttggc ttttttaatg gccttgtcga tggcggcaac
     2581 gtcgttgccg tcggcgactt tgagcacctg ccagccgtag gccttaaaac gctgttcgat
     2641 gttttccgtg gaagaaaggg ccgcggatcc cgccagcgtc actttattat cgtcatacag
     2701 aacaatcagt tttcccaagc gcagatggcc ggccagagcg caggcctcac cagccacgcc
     2761 ttccatcatg tcgccgtcac cggccatgac ataagtgaaa tgatcgacta ttttctgatc
     2821 ttccccgtta aaacgcgcag ccagatgcgc ttcggcaacc gccataccca ccgcattggc
     2881 cagtccctga cccagcgggc cggttgtgac ttcgacaccg ggcacatgtt tgtattccgg
     2941 atgccccggc gtgcggcttc cccactgccg gaaagtttta atatcgtcga gcgtcaaatc
     3001 gtaaccggtc agatgcagaa gagcgtataa aagcatggag gcatgaccgg cggaaagcac
     3061 aaaacggtcg cggtcgggcc attgcggatt tttggggtta tgtttcaggt gttttgtcca
     3121 gagcgtaaac gctgcggctg ctgcgcccag cggcataccg ggatggccgg atttggcctt
     3181 ttcgatcgca tcggcagcga gaaagcggat ggcatcaata cattgtttct ctaactcgtt
     3241 tttatcagac atgaagactc ctttaattat cgtaaggatg gtgatgtact aacacatttg
     3301 aagttttttt taaacttttc ttggaataaa ttttaccgcg gtgcacggaa aatgattgca
     3361 aaataaacat ttagagatta tgcattataa acaataagtt agcttatgtc taaaccaatg
     3421 attcaactca tcaaaataaa cgggggaagt tatcatatat gacaatttcc gcatttgaac
     3481 cggctacatt ggcaggaatc aaattgaaaa accggataat ccgttcggcg acctatgagg
     3541 gcatggccga tgaaaaagga ttccccaccg aaaaactaaa aaaactttac atcaatctgg
     3601 caaaagggga tgtcggcgca atcataaccg gatacgccgc tactcaggcg gacggcaagg
     3661 tcagcaacgc catggccatg gtcgatcatg acgacaaaat caatgttttt aaagaaatta
     3721 cagatgccgt tcatgaatac ggcacgccgg ttatcatgca aatcgcccac tgcggcaggc
     3781 aaacccgctc cagaataacc ggctttccga ccgttgcccc ctccccggtg cgcaatatgt
     3841 atttcatgga ggataagccg aaggccttat ccgaagaagg catcaacgaa atcatcgaca
     3901 attttgttgc cgccatcgtc cgggcaaaaa aggccggttt tgacggggtc cagcttcatc
     3961 tggcgcacgg ctacctgctg tccgattttt tatcctcgca tgcaaatcgc cgtaaagacc
     4021 gctggggcgg ctcaacagaa aacaaatacc gcatcgtcgg cgagattttc aaaagagcca
     4081 ggggaaaagt cggcgactac ccgatccttg taaaaatgaa cgcctatgac gggcggcgaa
     4141 aaggcatgag aattgaagaa gccgttgtca tcgcccggat gctggaaaac tccggctgcg
     4201 cggccatcga agtgtcctgc ggttgcgtgg aggacggcct tttcaccatc cgcggagaaa
     4261 agcttcccgt ggaagccgtc gtggaataca atttcaattt caaaaattat ccggcctttg
     4321 cgaagaaaac catcgtctgg tttgccaaaa catttctgaa aacgcccaaa ccgctgttga
     4381 aatacaatct cgacgcggca atgcaaatta aaaaagccgt gaacattccc gtgatcgttg
     4441 tcggcggcat caacaatgtc gaagatgttg aggacattat cgggaatgat aaggcggatt
     4501 ttgtctccat gagccggccg tttatcatcg agccggacat tgtgagcaaa ttcaaaaacc
     4561 ggacgcagac tacatcaaaa tgcatcatgt gcaatttttg cgtgatcacc atcgagaaag
     4621 aaccgctgaa gtgccgctac ggcaaactgc cgaaaacaaa a
//
LOCUS       DAOSME010000114         4613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_20295_length_4613_cov_20.669373, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000114 DAOSME010000000
VERSION     DAOSME010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20295_length_4613_cov_20.669373"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1144)
                     /gene="bamD"
                     /locus_tag="PLG98_10880"
     CDS             complement(41..1144)
                     /gene="bamD"
                     /locus_tag="PLG98_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOE33810.1"
                     /translation="MHAKKFVKTAPLKSVLTLLLLSLILGGCSSFSLFGKRDLPQASP
                     EGLYATAAEEYKDGNYKKAREYFSRVKEEYPLHELSVLAEIGIADSLYSNKDYEEAKD
                     AYSDFISLHPINENVPYAVYQTGMCHYHRMEAIDRDQTETINARKEWEKLIARYPESK
                     FSIMAEKMLKEVKQRLAEREFYIGNFYLNHKKYKAALARFEKIAAEYSNVGLDYKIEN
                     YIKEAKTGVDEEEKEKLREDEAQKSKEQDTIKKEEEKKKIAEEKRLKEEEAKRVKEEA
                     RIKKEEEKKRKVEEKRLQEEEAKRVKEEARIKKEEEKKKKAEEKRLREEEAKRVKEEA
                     RIKKEEEKKRKAEEKRLKEEEEKRLKEQAKMKN"
     gene            complement(1176..1505)
                     /gene="trxA"
                     /locus_tag="PLG98_10885"
     CDS             complement(1176..1505)
                     /gene="trxA"
                     /locus_tag="PLG98_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE33811.1"
                     /translation="MSSELLAAATDSNFENEVLKSDKPVLVDFWAPWCGPCKAIGPIV
                     EEIAGQYKDSVKIMKLNVDDNQKMASHFGVLSIPTLILFKEGKVLEKIVGLVPKARLE
                     DLIKKAL"
     gene            1650..2360
                     /locus_tag="PLG98_10890"
     CDS             1650..2360
                     /locus_tag="PLG98_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOE33812.1"
                     /translation="MGMLGIISTRLVLEQSELLKKSSAKPMENGFGKTTVFTTDKTAL
                     ILRHGMDPDNYILPHLINHPANLQALKDIGVSEIVSINSTGSLKKEIKPGMIVVPDDF
                     LTLTPSPTIHVNRPVHIIPSLNEGIRKKLIRAALKNDFEVVQQGTYWQTQGPRLETKA
                     EISTMTRFADIVGMTMANEAVIAQEMDIPYACACSVDNFGNGLMAEPLSMEEIISHTR
                     KNAQRMIQLLDRYLQMFF"
     gene            complement(2362..2796)
                     /locus_tag="PLG98_10895"
     CDS             complement(2362..2796)
                     /locus_tag="PLG98_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33813.1"
                     /translation="MTHEDKGKYFKKHPEGTRIDDELKDEIISQVKNNNITCKKAEEI
                     ASALGCSLAETGRAIDILNINITKCQLGLFGYGDTKKIVQPVKEVAPELKESIMSALE
                     NERLSCAAVWGIAKKFNMPRMKVCAACEALDIKVKPCQLGAF"
     gene            complement(2821..3252)
                     /locus_tag="PLG98_10900"
     CDS             complement(2821..3252)
                     /locus_tag="PLG98_10900"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOE33814.1"
                     /translation="MVAYQLSIFAENKPGKLARVTGVLAKEKINIRATTIATADTFGV
                     INLIVDDPRRAEKALSDAGMTVHLREVLAVLISDQPGALDSLTQLFQKEGVNVNNAYG
                     FVLEGSKKAVFVVDVDQIRKTEKLLEHEGFKTLDTQSLSSV"
     gene            complement(3276..>4613)
                     /locus_tag="PLG98_10905"
     CDS             complement(3276..>4613)
                     /locus_tag="PLG98_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOE33815.1"
                     /translation="ALAVPLNYRYTEDEIKYCLDLAECDVLVFGPEFTERIDAIKDTI
                     PNVKIRLFVGQDCPAFAESYYALTACCSSANPKISLFDGDDAAIYFSSGTTGFPKAIL
                     HSHSALMMSCEVEMNHHGQTRTDNFLCIPPLYHTGAKMHWFGSFLAGSRAVLLRGVKP
                     EWIFKAVSEEQITIVWLLVPWAQDILDAVERGDIKLENYKLDQWRLMHIGAQPVPPSL
                     IKRWKKYFPVHEYDTNYGLSESIGPGCVHLGTENIHKVGAIGVAGYKWDVQIVDEKGT
                     PVKQGEVGELCVKGAGVMKCYYRNPEATAEVLKNGWLYTGDMARMDEDGFIYLVDRKK
                     DVIITGGENIYPVQIEDFLRTHDAIKDVAVIGLPDQRLGEIVAAVIEFKPGFAATESE
                     IAEFCNALPRYKRPRRIIFEKVPRNPTGKIEKPKLREKYFGIGSLVEAQTENL"
BASE COUNT         1055 a         1211 c          943 g         1404 t
ORIGIN      
        1 ttacagtctc taattcatat acccgcatat ccttctgttt ttaattcttc atcttcgctt
       61 gttctttaag tcttttttcc tcctcttctt tcaaacgctt ttcttcagct tttctttttt
      121 tctcttcttc tttctttatt ctggcttctt ccttaactct ctttgcctct tcctccctca
      181 atcttttttc ctcagctttt ttcttctttt cttcttcttt ctttattctg gcttcttcct
      241 taactctttt tgcctcttct tcttgcaaac gtttttcttc aacttttctt ttcttctctt
      301 cctctttctt tattctggct tcttccttaa ctctttttgc ctcctcttct ttcaaacgct
      361 tttcttcagc tatcttcttt ttttcttctt ctttctttat tgtatcctgt tctttacttt
      421 tttgggcctc atcctctctt aatttctctt tctcttcttc gtcaacaccg gtttttgctt
      481 ccttgatgta attctcgatc ttgtaatcga gcccgacgtt gctgtattcg gcggctatct
      541 tttcaaaacg cgctagggct gctttatatt ttttgtgatt gaggtaaaaa ttcccgatat
      601 agaattcgcg ttcggccagc ctctgtttca cttctttcag catcttttcc gccataatgc
      661 tgaatttact ctccggatag cgggcaatta acttttccca ttcttttctg gcattgattg
      721 tttccgtctg atcccgatct attgcttcca tacgatgata atgacacatt ccggtctgat
      781 agacggcata aggaacattt tcattgatgg gatggagaga aataaaatcg ctgtaggcat
      841 cttttgcctc ttcataatcc ttattgctgt atagcgaatc ggcaatgcct atttccgcca
      901 gtactgacag ttcgtgcagg ggatattctt ccttgacacg tgaaaaatat tcccgtgcct
      961 ttttgtaatt cccatcctta tattcctcgg cagccgttgc atagagtcct tccgggctag
     1021 cctggggaag gtcgcgtttg ccgaaaaggc tgaaagacga acaacctccg agaattagtg
     1081 ataataacaa aagcgtcaaa acacttttta atggcgctgt ttttacgaat ttctttgcat
     1141 gcatcaacgt taacctctcg gactgtattt tcggcttaca atgctttttt gattaaatct
     1201 tcaagtctgg ctttcggcac caggccgacg atcttttcca gaactttacc ctctttaaag
     1261 agaatcagcg tgggaatgct gagaacgccg aaatgcgacg ccattttctg attatcatcg
     1321 acgttcagct tcattatttt cacactgtct ttatactgtc cggctatttc ctcaacaatc
     1381 ggaccgatgg ctttacaggg accgcaccac ggtgcccaga aatcaacaag cacgggtttg
     1441 tcgcttttca aaacttcatt ttcaaagtta ctgtcggtgg cagcagccag tagttcactg
     1501 ctcattataa agccctcctt agatttttag agtgaatctt tatgtcacaa taagccccat
     1561 aaagtcaaat gcaatctggc aacagatccg caacgacctt tgacggtttc ccgttaattt
     1621 gctatataat cgggctcaaa ggagaatcta tgggcatgct gggtattatt tctacaaggc
     1681 tggttctgga acaaagtgaa ttgctgaaaa aaagttccgc taagccgatg gaaaacgggt
     1741 tcggtaaaac gactgtgttc acgacggata aaacagcgct gattctgaga cacggcatgg
     1801 atccggacaa ctatatttta cctcatttga tcaatcatcc ggccaatctt caggcgctga
     1861 aagatatcgg ggtttccgaa attgtaagca tcaattccac cggatcgcta aaaaaagaaa
     1921 tcaaaccggg gatgatcgtc gttccggatg attttctgac tctgactccc tcgccgacca
     1981 ttcacgtgaa cagacccgtc cacattatcc cgtcattaaa tgaaggcatt aggaaaaagc
     2041 ttatccgagc cgctttgaaa aatgatttcg aggtggtgca gcaaggaacc tactggcaga
     2101 cacagggccc tcggctggaa acaaaagccg aaatcagcac gatgacccgc tttgccgaca
     2161 ttgtcggcat gaccatggcc aatgaagctg tcattgctca ggaaatggac attccctatg
     2221 cctgcgcctg ttccgtcgat aacttcggaa acggcctgat ggctgaacct ttaagcatgg
     2281 aagaaatcat cagccacacc cgcaaaaacg cacagcggat gattcaactg ctggaccgtt
     2341 acctgcaaat gtttttttaa atcagaaagc acccagttga cagggcttaa ccttgatgtc
     2401 cagcgcttcg cacgcggcgc ataccttcat ccgcggcata ttaaatttct tcgcaatgcc
     2461 ccagacggcc gcgcaggaca gcctttcatt ttccagagcg gacatgatgc tttctttcag
     2521 ctccggcgcc acctccttca ccggttgaac gatttttttg gtatcaccgt aaccgaaaag
     2581 tcccagttgg catttggtaa tattaatatt cagaatatct atcgctctgc ccgtttctgc
     2641 cagactgcat cccagtgccg aggctatctc ttccgccttt ttgcaggtga tattattatt
     2701 tttaacctga ctgatgatct catctttcaa ttcatcatcg attcgggtcc cttccggatg
     2761 ttttttaaaa tatttgcctt tatcctcgtg cgtcatgttg cctcctcctt gttgaatttt
     2821 tcatacagaa gaaagtgact gagtgtccag tgtcttaaaa ccttcatgct ccaaaagttt
     2881 ctccgttttc cgtatctgat cgacatcaac gacaaatacg gctttcttcg acccctccag
     2941 cacaaagccg taggcattat tgacattgac cccttccttt tgaaatagtt gagtcagact
     3001 gtccagcgcg ccgggttgat cggaaatcaa aaccgccaac acttcccgca aatgcaccgt
     3061 catgcccgcg tcggatagtg ctttctcggc acgccgggga tcgtcgacga tcaggttgat
     3121 cacgccaaac gtatccgccg ttgctatggt tgtggcgcgg atattgattt tttcttttgc
     3181 cagaacaccg gttacccggg ccagtttccc gggtttgttt tccgcgaaaa tagaaagctg
     3241 ataagcaacc attcccttct ctccttcctg ttgagttaga ggttctccgt ctgcgcctcc
     3301 acaaggcttc caattccgaa atacttttca cggagtttcg gcttttcgat ttttcccgtg
     3361 ggattgcgag gcaccttttc aaaaataatc ctgcgcggac gcttatagcg cggcaatgca
     3421 ttacagaatt ccgcaatttc ggattctgtc gcagcaaaac ccggtttgaa ttcaatgacc
     3481 gccgcaacga tttcccccaa gcgctgatcg ggaagtccaa tgaccgcgac gtccttaatg
     3541 gcgtcatggg tgcgcaggaa atcctcgatc tgcacgggat aaatattttc gccgccggta
     3601 ataatgacat cctttttgcg gtccaccaga taaatgaatc cgtcttcatc cattctcgcc
     3661 atatctccgg tgtaaagcca gccgtttttg agaacttcag cggtggcttc cggatttctg
     3721 taataacatt tcatcacacc cgcgcctttg acgcaaagtt cacccacttc accctgcttc
     3781 accggtgtgc ctttttcatc aacgatctgc acgtcccatt tataaccggc tacgccgatg
     3841 gcgccgacct tatggatatt ttcagtgccg agatggacac accccggccc gatggattcc
     3901 gaaaggccgt aattggtgtc gtattcatgt acgggaaaat attttttcca gcgtttaatc
     3961 aggctgggcg gaaccggctg tgcgccgatg tgcatcagcc gccactgatc gagcttgtaa
     4021 ttttccagct tgatgtcacc gcgttcgacg gcgtccagaa tatcctgcgc ccacggaacc
     4081 agcagccaca cgatggtaat ctgttcttcg gaaaccgcct tgaaaatcca ttccggcttc
     4141 acaccgcgca gcagaacggc gcggcttccg gccaggaagc tgccgaacca gtgcattttt
     4201 gcgcccgtat gataaagagg cgggatgcag agaaaattgt ctgtgcgcgt ctggccatga
     4261 tgattcattt ccacttcgca cgacatcatc agggcgctgt gcgaatgaag aatggccttt
     4321 gggaaaccgg tcgtgcctga cgagaaataa atggccgcat cgtcgccgtc gaaaaggctg
     4381 attttcggat ttgccgagga acagcaggcg gtcagcgcat aataactttc agcaaacgcg
     4441 ggacaatcct gacctacaaa cagccggatc ttgacgttcg gaatcgtatc cttgatcgca
     4501 tcgatccttt cagtaaattc cggtccgaag accaacacat cgcattcggc cagatccagg
     4561 cagtatttga tttcatcctc ggtataacgg tagttgagcg gcacggccag cgc
//
LOCUS       DAOSME010000115         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_20388_length_4597_cov_26.292602, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000115 DAOSME010000000
VERSION     DAOSME010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20388_length_4597_cov_26.292602"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..3710
                     /locus_tag="PLG98_10910"
     CDS             75..3710
                     /locus_tag="PLG98_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33816.1"
                     /translation="MVDIDALTKKYGKQKEEVQEIEKSFMRITDAVYRITYSGLELGE
                     MLGLDPDLIRGFNALIGSVLDMVDAANLLQDALDGVAKGGSDTADAMADASASVGNAS
                     MGIVSAFFAIASAMLKIGTAIADMKNAREDAKIEAYIDDVNKRLETLQRNLENIANIT
                     GIGDSWFTEDNFNNVRMLVDSINEAIIGAQQFADAMEQSFIGIPDMIKANETFKALQE
                     GYAALKGKLDMGGLTFDVGEIDKLIELAEQFKDSGKLAEQAAADVDAFIAAMKEAKKY
                     YDELGSLIESMMSNVSSGLLSTVSSVWKEFRNKGVDAIGEVTSAAKKSITEIIDQMVS
                     QQIWAETMAPYFTQLGKDLTNAIAGGVDLMDVFDNFWTKMQGGLSDYTRMYESFLQSA
                     ESHGWNMGSDSTGTGDTEAAKDSIKAMREELAMLQEQWESLSDAERKSGDGKELFGSI
                     EDLEKKIEAAENLYNITNNIAGSINDLNAQLSRLNEEWNALSPEDRNGAIGQKIQSDI
                     AYYEDLLNRAQGMQESELEALQSLMESAGAKYGLHQKWAELYGSEEADRMMGQMLDDA
                     QGYVDQLRSGINNLRTRVTEGAATDEEISQLEAWEQQLNDILSQGTQAAINAANEAFQ
                     GWNDALDNMLNGIENDYDRLAILQGEYLSALENYEGLDAGPMKDAAKEYLDYLEGMLK
                     DQSALIEKDLQERYKTQEQASAETLRTFEDDLKYARDILQDEALTKEIERQRNEFLSE
                     QAAAALEASEEYKLIFGDLTNISEEAFRDAMDKIKKSIEESTDLTDEAKAEILKNLDA
                     IGAGFDDSLGEKRINKIKQSFDDLVSVMNDTVSLMQSLGADDSVVQMLQGVVKVVEGY
                     RDLKLAIEEAALAQTALSLSNVFTSIVAVINGLIMALSALGVKLVQLVDVDSISGLAK
                     AYDKLTDAISRSVGAQRLQKQEAARQNLEQQKAEVERALAREKAKWGFKLDLGIFGSY
                     QILGPDQGAVNEMEAQIADLNKALNQLGDTMKQDYLQTDAQSFSSELSNILTSEYDSY
                     ADMMADVEKLTQRTLNNITRQWLTAHFLENSIQDALDALYGSGKEPTAQAYETFQRKV
                     NEASENFMREMDRMNLMFGGEDTASGTSNAGLISKSITENTATWIKGLMMNQNLELND
                     MNKTMQYISKYSERTANGVEDLTRINSQIAQHTSVLPKMAQSMVNLAMDVSQIKNKVG
                     LSIENRN"
     gene            3925..4506
                     /locus_tag="PLG98_10915"
     CDS             3925..4506
                     /locus_tag="PLG98_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33817.1"
                     /translation="MNFEKLKNELFDLYEPNELVALCDEMYTSFLAHDRGNKSENEAG
                     FYLTNTMREFIREFHPDSDNGGETGLTLESVSRKDQGAKKPISKGSNQQTIKERMSAV
                     TISDSTRDFLRLIGGLDDLMMDEFWYAEMKRLGYDENSEETSKMWEKHVNEDKSMTAI
                     TDGYTSLRKLIVQHIASFVVDRLEDDPYEIKEI"
BASE COUNT         1429 a          880 c         1226 g         1062 t
ORIGIN      
        1 agatgtcaaa aacacccatt ccatccgtta caggtggaga tgtcacggga aaagtcacca
       61 caacgaagca gcaagtggtt gatatagatg ctcttaccaa aaagtacggc aagcagaaag
      121 aggaagtcca ggaaattgaa aaaagtttca tgcggattac tgatgcggta tatcgcataa
      181 catactcagg tcttgagctt ggcgaaatgc tcggtctcga tcccgatctt attaggggat
      241 ttaatgcttt gattggttcc gtattggaca tggtagacgc agctaactta ttgcaggatg
      301 cgcttgacgg ggtagcgaag ggtggtagtg ataccgcaga tgctatggca gacgcttcag
      361 caagtgttgg aaacgcatct atgggcatag taagtgcctt ttttgccatt gcttctgcta
      421 tgctcaaaat aggcacagca atagccgaca tgaaaaatgc cagggaagat gcaaagattg
      481 aggcatatat tgatgatgta aacaagcgac tggaaacttt acagcgaaat cttgagaaca
      541 ttgccaacat aacgggcata ggagattcct ggttcaccga agataatttc aataacgtaa
      601 ggatgctggt tgattcaatc aatgaggcga ttatcggtgc tcagcagttt gctgatgcta
      661 tggaacaatc gttcataggt attccagaca tgattaaggc taatgaaacg ttcaaggctt
      721 tgcaggaggg atatgccgca ttgaagggca aacttgatat gggtggtctt acgtttgatg
      781 ttggcgaaat cgataagctg attgagctgg cggagcagtt taaggacagc ggcaagttgg
      841 cagaacaggc ggcggcagac gtggatgctt ttattgcggc catgaaagag gcaaagaagt
      901 attacgatga gctgggaagt cttatagaat ccatgatgtc caatgtgtca agcggcctct
      961 taagcacagt tagttccgtg tggaaggagt tccgcaataa gggcgttgat gccattggag
     1021 aggtgacaag tgcggcaaag aaaagcatta cggaaatcat tgatcagatg gtttcgcagc
     1081 agatatgggc ggagaccatg gctccctact ttacacagct tggaaaagac cttacaaacg
     1141 caatagccgg tggcgtggat ttgatggatg tctttgacaa tttctggaca aagatgcagg
     1201 gtggtctttc cgactatacc cgaatgtatg aatccttttt acaaagtgcc gaatctcatg
     1261 gttggaatat gggaagcgat agcaccggaa caggagatac agaagccgct aaagattcca
     1321 ttaaggctat gcgtgaggaa ttggcaatgc tccaggaaca atgggaaagc ctgagcgatg
     1381 cagaacgcaa aagcggagac gggaaagaac tgtttggaag cattgaggac ctggaaaaga
     1441 aaatagaggc agccgaaaac ctatataaca taaccaacaa tattgcgggt tccataaacg
     1501 atttgaacgc ccagctttca cgtttgaatg aggagtggaa cgccctttcc cctgaagatc
     1561 ggaatggagc catagggcaa aaaatccagt ccgatatcgc ctattatgag gacttgttga
     1621 atagggcaca agggatgcag gaaagcgaac tggaggccct gcagtcactc atggaatcag
     1681 cgggggcaaa atatggtctg catcaaaaat gggccgaact gtacgggtcc gaggaagcag
     1741 acagaatgat ggggcaaatg ttggatgatg cccagggcta tgtggatcag ctgcgatccg
     1801 gcataaacaa ccttagaaca agggtgacag agggagcggc cacagatgag gaaataagcc
     1861 agttggaagc atgggagcaa caattgaatg acatactgtc tcagggaaca caggctgcca
     1921 taaacgccgc aaatgaagct ttccaggggt ggaatgatgc cctggacaac atgctgaacg
     1981 gcatagagaa tgactacgac agactggcga tcctgcaggg tgagtatctc tctgcgcttg
     2041 aaaattacga agggctggat gcggggccga tgaaagatgc tgcaaaggaa taccttgatt
     2101 atttggaagg tatgttgaag gaccaatccg cactcattga aaaggacctg caggagcgtt
     2161 ataaaacaca ggagcaagcc agcgccgaga cgttgaggac ctttgaagat gatttgaagt
     2221 atgcccgtga tatcctacag gatgaggcgc taaccaaaga gatagaacgc cagcggaatg
     2281 agtttctttc cgaacaggca gctgcagccc ttgaagccag cgaggagtat aagctaatat
     2341 ttggggatct taccaacatt agtgaagaag ctttccgtga tgcgatggat aagattaaaa
     2401 agtcaataga agaaagtacc gatttaaccg atgaggcaaa ggccgagatt ttgaaaaacc
     2461 tggatgccat tggtgccggg tttgatgatt cgctgggcga gaaacgtata aataagatca
     2521 aacaatcttt tgatgatctt gtttcggtaa tgaatgacac ggtgtctctc atgcagtcct
     2581 taggtgctga tgattccgtt gtccagatgc tgcagggtgt tgttaaggta gtggaaggct
     2641 accgtgatct aaagcttgct attgaggagg cggcattggc acaaacagcg ttaagcttgt
     2701 ccaatgtgtt cacttctatt gtagctgtga taaatggtct tattatggcc ttatccgccc
     2761 tgggtgtgaa gctggtccag ttggtggatg tggacagtat ttccggactg gccaaagcct
     2821 atgacaaact tacggatgcg ataagcagat ccgttggcgc tcaaaggcta cagaagcaag
     2881 aggcggcacg gcagaacctg gagcagcaaa aagccgaggt tgaacgggcg ttggcacggg
     2941 agaaggccaa atgggggttt aagcttgatt tagggatatt tggaagctat cagatacttg
     3001 gacccgacca gggagcagta aacgagatgg aggctcaaat agcggattta aacaaggccc
     3061 tgaaccagct tggcgacacc atgaagcagg actatctgca gacagatgca caaagctttt
     3121 catccgagct gtccaacatc ctgacatcag agtatgattc gtatgccgat atgatggccg
     3181 atgtggagaa gcttacacaa aggacattga ataatattac ccgtcaatgg cttacggctc
     3241 acttcctgga gaatagtata caggatgccc tggatgcgct ttatggcagc gggaaggaac
     3301 cgacagccca ggcctatgag acattccaga gaaaggtaaa cgaggcgtca gagaacttca
     3361 tgagagagat ggaccgcatg aatttgatgt ttggtggcga ggacaccgca tcaggcactt
     3421 ccaatgcagg attgatttcc aagtccataa ccgaaaacac ggccacgtgg ataaagggac
     3481 tgatgatgaa ccagaatttg gaactgaatg atatgaataa gaccatgcaa tacataagca
     3541 agtactccga aagaaccgca aatggcgttg aagacctgac ccggataaac agccagatag
     3601 cgcaacacac aagcgttctg ccaaaaatgg cgcaaagtat ggtcaacctg gcgatggatg
     3661 tcagccagat aaagaataaa gtgggtttgt cgatagaaaa ccgcaattaa ttccctttcc
     3721 tccattttcc gggggcacct gaaacacggt gtcccttttt tatgtcaaat acattgatta
     3781 ctaagggtaa aaagcgataa ggtagggcat agtgccccac atttataagt tactgaaaac
     3841 aatcgaatta tgcaatagca aattgtgttg ttagtgtcga aagtttgtct tttctttgca
     3901 ccatcaacaa taaaaaatac tgtcatgaac tttgaaaaat taaaaaacga actgttcgac
     3961 ttgtacgaac ctaacgaact tgtcgcattg tgcgatgaga tgtacacatc attcctggcc
     4021 catgatcggg gaaacaaaag tgaaaatgaa gccggatttt acctgacaaa cacaatgagg
     4081 gagtttatca gggagtttca cccggattcg gataatggcg gagaaacggg attgacctta
     4141 gaaagcgtat cacggaaaga ccagggtgcc aaaaagccca tatctaaggg gtctaatcag
     4201 caaacaatca aagaacgtat gtctgcagta accatttcag attcaaccag ggattttcta
     4261 aggttaatcg gaggattgga tgatctgatg atggatgagt tttggtatgc cgaaatgaaa
     4321 aggcttggct atgatgaaaa ttccgaggag accagcaaga tgtgggaaaa gcatgtgaat
     4381 gaagataaat ccatgactgc gataactgac ggatatacca gtctcagaaa gcttatcgtt
     4441 cagcatatcg catcctttgt ggtcgatcgc ctggaagatg atccctacga aataaaagag
     4501 atataaaaat ttttgtatct ttgtatcagc aacacacata aaataaaaat gtcattgaaa
     4561 atgggcgctc aaatgggcgc ctttttttat taagtcg
//
LOCUS       DAOSME010000116         4550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_20660_length_4550_cov_22.604894, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000116 DAOSME010000000
VERSION     DAOSME010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20660_length_4550_cov_22.604894"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..339
                     /gene="rpmA"
                     /locus_tag="PLG98_10920"
     CDS             85..339
                     /gene="rpmA"
                     /locus_tag="PLG98_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOE33818.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRRGIKVFGGEQIHSGSIIVRQVGTK
                     IHPGNNVGMGKDFTLFSLINGVVKYERLDKTRKKVSVYAA"
     gene            535..1530
                     /gene="obgE"
                     /locus_tag="PLG98_10925"
     CDS             535..1530
                     /gene="obgE"
                     /locus_tag="PLG98_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOE33819.1"
                     /translation="MKFVDEAKIYVKAGHGGSGCVSFRREKFVPKGGPDGGDGGKGGD
                     VIFRTTESHHTLLDLKYKQHQIAKNGGHGSGNHRTGKSAPDLEISVPVGTIIKNFDTG
                     EELADLSKAGQTYIAARGGIGGKGNSHFATSTHQTPRFAQEGMEGDEFTLKLELKLLA
                     DVGIIGFPNAGKSTFISRVSAAKPKIADYPFTTLTPHLGVVQYADDKNFVIADIPGLI
                     PGAHEGMGMGDKFLKHVERTSLLLHIIDVSMTPHTDAWSNFSTINDELKKYNPALAEK
                     EQIAAINKIDLPFVREKIKKEVALFKKKGIILHAFSAVTGEGVKEILNRIAEKLK"
     gene            1550..2695
                     /gene="proB"
                     /locus_tag="PLG98_10930"
     CDS             1550..2695
                     /gene="proB"
                     /locus_tag="PLG98_10930"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOE33820.1"
                     /translation="MENIRQNIITRAKKILIKVGSAVLTGDDGLDLKIIDSLVLEMAN
                     LAKKGYAVVLVTSGAIVSGKHRLKITENLKSIPEKQAAAAIGQGRLMRVYSKAFEKNG
                     IYVAQILLTLSDLTDRQRYLNIRNTLSTLMAWGTIPIINENDTVAVDEIKFGDNDNLA
                     AMIANIIEADLFINLTSIDGLYTCNPASSKKSKLISVVSEFSEEIEAAATEETSSSGT
                     GGMKSKVQAAKKVTAIGIPCIIAPGKKKNILTDIMTGREIGTLFLPMTDRLNSKKYWI
                     AFTLRPRGKLVIDDGAKKALLEEGKSLLPSGIIDIEGRFDHGDPVICVDNNGAILAKG
                     LVNYNSIEINKIKGLKTKQIGQVLGHKDYDEIIHRDNMAVTGQHYKK"
     gene            2715..3194
                     /locus_tag="PLG98_10935"
     CDS             2715..3194
                     /locus_tag="PLG98_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33821.1"
                     /translation="MLKDYQMQLLGFLEQLEMEISNLYKSFAEQFPGHQDLWAEMSRE
                     ELQHAEYVRTLKAFALSGHVTFDEKMTKTYTIRSIIDDIRDKYKKTENHQYSVVNALS
                     FSLSLEQSIIEHKFYNYFSSNDPGAVLLINNIREETSAHESQVKKALDEEKKRITVR"
     gene            complement(3221..3631)
                     /locus_tag="PLG98_10940"
     CDS             complement(3221..3631)
                     /locus_tag="PLG98_10940"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOE33822.1"
                     /translation="MSEKYYKLFSDGACRGNPGIGGAGAVITDDADHVIWEGKEYLGH
                     CTNNIAEYKALILGLKGALSQGYRNLQIFLDSELLAKQINGAYRVKNENLQTLMKEVR
                     HLLVSFDRVEVKHVARLYNSHADKLANLAIDEKN"
     gene            complement(3637..4356)
                     /locus_tag="PLG98_10945"
     CDS             complement(3637..4356)
                     /locus_tag="PLG98_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOE33823.1"
                     /translation="MKEQLLLLIKIQECDSELVKLSAKKKTLPEKIETLNKEFNAFED
                     GIKTSKVKYDELKLRHTDSENKIKKINESIVKTKERMLEVKNNKEYQAMLKEIEIAEK
                     SRGEIETSIISILDELDKLSVLVKKDDDVLKERRHQYEQEKKTIEDELNAVDTDAANW
                     EQKRGDLQKNVPEDLLVRYERIKKRNRGVGVISVWKSVCNGCHMNIPPQLYNEVQKSE
                     ELFSCPNCNRIIYFKDMVKPS"
BASE COUNT         1296 a         1061 c         1000 g         1193 t
ORIGIN      
        1 aaaggtttcc ataaaaaaac cggtcatcgt caaaatctaa ccagcatgaa aattaagaaa
       61 atttcgatat aagcggagga agtcatggca cataaaaaag gtcagggaag ttcccgcaat
      121 ggcagagaca gcaattccca gagacgcgga attaaggtat tcggcggaga acaaattcac
      181 tccggctcga tcattgtgcg acaggtagga acaaaaattc acccgggcaa caacgtggga
      241 atgggcaaag atttcactct tttttccctg attaacggcg ttgtaaaata tgaaagactt
      301 gataagacga ggaagaaagt aagcgtttac gcagcataag aatattcgat cggatcgatt
      361 acaaccattt aaggcgctta cttcaagcgc cttttgtttt taatgaggct ccaattccaa
      421 aaacgaagtg cattggaatt ggctagccgg attatcccgc gtatgcggaa taagggatca
      481 acgtattaaa tatagtttat cttaactcaa aagaacatac ggctgaacaa ggttatgaaa
      541 ttcgtcgatg aagcaaaaat ttatgtcaag gccggacacg gcggaagcgg ttgcgtgagt
      601 ttccggaggg aaaaatttgt cccgaaaggt ggtccggacg gaggagacgg cggcaaaggc
      661 ggcgatgtca tctttcgcac aacggaaagt catcacacgc tgttggactt aaaatacaag
      721 cagcatcaaa tcgccaagaa cggcggccac ggttccggca atcaccgcac cgggaaaagt
      781 gctccggatc tggaaatctc cgttccggtg ggaacaatca tcaaaaattt tgataccgga
      841 gaagagcttg ccgatctgtc aaaagcggga cagacgtata ttgccgcacg tggcggcatc
      901 ggcggcaagg gcaattctca ctttgccacc tcaacgcatc agacgccccg cttcgcccag
      961 gaaggaatgg aaggcgatga attcacgctc aaactcgaac tgaaacttct ggccgatgtg
     1021 ggcattatcg gtttccccaa tgcgggcaaa tcgaccttta tctcccgtgt ttccgcagcc
     1081 aaacccaaga ttgccgatta ccccttcacc acgcttacgc cgcatctggg cgtcgtgcaa
     1141 tatgcagacg ataaaaattt tgtcattgcc gacattcccg gactgattcc gggcgctcat
     1201 gagggcatgg gcatgggcga taaatttctg aaacacgtcg aaagaacgtc attgctgttg
     1261 catattatcg atgtctccat gaccccccat accgacgcct ggtccaattt ctcaacgatt
     1321 aacgacgagc tgaaaaaata taacccggct ctggccgaaa aagagcaaat cgcagcgatc
     1381 aacaaaattg atcttccgtt tgtccgggaa aaaatcaaaa aagaagttgc tctattcaaa
     1441 aaaaagggca taatattaca cgctttttca gcagtcaccg gcgaaggggt aaaggaaata
     1501 ttaaacagga ttgctgaaaa gttaaaataa accggttaga gttgatgtca tggaaaatat
     1561 tcggcaaaat atcatcacgc gcgcaaaaaa aattctgatc aaagtcggtt cggccgtact
     1621 gacgggagat gacggtctcg atctgaaaat catcgattct ctggtgctgg aaatggcgaa
     1681 tctcgccaag aaaggttatg cggtcgtgct ggtaacctcc ggagcgattg tctcgggaaa
     1741 acaccggttg aagatcacgg aaaatctgaa aagtattcct gaaaaacagg ctgccgcagc
     1801 catcggtcag ggcagattga tgcgcgtcta ttccaaagcc ttcgagaaaa acggtattta
     1861 tgtggcgcag attcttttga cgctttccga tttgaccgat cgccagcgat acctgaatat
     1921 ccgcaacacc ctttccacac tgatggcgtg gggaacgatt cccattatca atgaaaatga
     1981 cacggttgcg gtggatgaaa taaaattcgg cgacaatgac aatctcgccg cgatgattgc
     2041 taatattatc gaagccgatc tctttattaa cctgaccagc attgacggcc tctacacctg
     2101 caatccggcc tcatccaaga aatcgaaatt gatcagtgtc gtctctgaat tttctgaaga
     2161 aattgaagcc gcggcgactg aagaaacatc ctcctcggga acgggaggaa tgaaaagcaa
     2221 ggttcaagcc gccaagaaag tgacggctat cggcattccc tgcatcatcg cgcccggaaa
     2281 aaagaaaaat atcctgacgg atattatgac gggccgcgaa atcggaacct tatttttacc
     2341 catgacggat cgtttgaaca gcaagaagta ctggatcgct ttcaccctgc gtccccgcgg
     2401 caaactcgtg atcgacgacg gggcgaaaaa agcactgctg gaagagggca agagccttct
     2461 tccttcagga atcattgaca ttgaggggcg ctttgaccac ggagacccgg tcatctgcgt
     2521 ggacaacaat ggtgcgatcc tggcgaaagg actggtgaat tataattcca tagaaattaa
     2581 taaaatcaaa ggattaaaaa caaaacaaat cggtcaggtt ctcggacaca aggattatga
     2641 tgaaatcatt catcgcgaca atatggccgt caccggacaa cattacaaaa aataacgcgg
     2701 aaaagtaacg tcacatgctg aaagattatc aaatgcagtt gctgggtttt ctcgagcaat
     2761 tggaaatgga aatctccaat ctctataaat cgttcgccga gcaatttccc ggacatcagg
     2821 atctctgggc tgagatgagc cgggaagaat tgcaacacgc cgaatacgtc cgcactttaa
     2881 aagcattcgc gttgagcggc catgtgacct ttgatgaaaa aatgaccaaa acctacacga
     2941 ttcgatcgat tatcgatgat atccgcgata aatataaaaa gacagagaac catcaatact
     3001 ccgtcgtcaa cgctttatcg ttcagcctca gcctggagca atccatcatc gaacacaaat
     3061 tttataatta tttcagttca aatgatcccg gtgcggtcct gctgatcaat aacatcagag
     3121 aagaaacctc ggcgcatgaa tcgcaggtaa aaaaagccct ggatgaagaa aaaaaacgga
     3181 taactgttcg gtaatgatct ttcgtttcaa tccctaaaaa ctaatttttt tcatcaatgg
     3241 ccaggttggc cagtttgtct gcatggctgt tgtacagacg cgcgacatgt ttcacctcaa
     3301 cgcgatcaaa agacaccaga agatgacgga cttctttcat gagtgtctgc agattttcat
     3361 tcttgacacg gtaggcgccg ttgatttgtt tggccagaag ctccgaatcc aaaaatattt
     3421 gaaggtttct atatccctga gacagggcac ctttgagtcc cagaataagc gctttgtatt
     3481 cggcaatatt attggtgcag tgaccgagat attcctttcc ttcccaaatc acatgatcag
     3541 catcatcggt gattacagcc cccgcgcccc cgatgcccgg attcccccgg caggcgccgt
     3601 cgctgaacag tttgtaatat ttttcagaca tatcccttat gatggcttga ccatatcctt
     3661 aaaatagata atccgattgc agttcgggca actgaacaac tcctccgact tctggacttc
     3721 gttatagagt tgagggggga tgttcatgtg gcacccgttg cagaccgatt tccagaccga
     3781 tatcacgcca acgcctctgt ttcgtttttt gattctttcg tatcggacca gcagatcttc
     3841 aggaacattt ttctgtaaat cccctctttt ttgttcccag ttcgccgcat cggtgtccac
     3901 ggcattcaat tcatcttcga tcgttttctt ttcctgttcg tattgatgtc ttctctcttt
     3961 caatacgtcg tcatcttttt tgaccagcac agaaagttta tccaattcat ccagtatgga
     4021 aatgatcgag gtttcaattt cgccacgcga tttttctgca atctcaattt ctttgagcat
     4081 cgcctgatat tccttattat ttttaacttc cagcattctt tctttggttt tgactatgct
     4141 ttcgttgatt ttcttgattt tattttcgct atccgtatga cgcaacttga gttcatcata
     4201 cttaactttg ctggttttta tgccatcttc aaatgcatta aattctttat tgagtgtctc
     4261 gatcttctcg ggcagtgttt ttttctttgc ggacaacttc accagttcgg aatcacattc
     4321 ctgaattttt atcagtaata ataattgctc tttcaatcat cttctccttc tttttttcaa
     4381 tccctgacat gaagttcagg gtcatttttt ttatttcgtc ttgctcttaa caagaatcca
     4441 ttccgcttcg ctgcaggaat agtatccaac cccgatacac cgggattgga tagttcggtt
     4501 tacgcttcac tatcgttcag cttgcctcac taccgcttgc gcttcgagct
//
LOCUS       DAOSME010000117         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_20733_length_4537_cov_22.526997, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000117 DAOSME010000000
VERSION     DAOSME010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20733_length_4537_cov_22.526997"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /gene="lepB"
                     /locus_tag="PLG98_10950"
     CDS             <1..567
                     /gene="lepB"
                     /locus_tag="PLG98_10950"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOE33824.1"
                     /translation="IIAILIALFIRTFIICAYKIPSRSMVPTLLVGDHILVNKFIYGV
                     KIPLLRRTIIPVSEPKRGEVVVFIYPNDRSKDFIKRVIGVAGDKIEIKNKKIFINDEE
                     FKDSYGIFSDSLVLPQSLQPRDNFGPIIVPEKSIFVMGDNRDESLDSRFWGFVNLKDV
                     EGKAFVIYWSWNRDENNLRWNRLGQLLH"
     gene            824..1372
                     /gene="pyrR"
                     /locus_tag="PLG98_10955"
     CDS             824..1372
                     /gene="pyrR"
                     /locus_tag="PLG98_10955"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOE33825.1"
                     /translation="MSNTKSTKTVMDKEGIERCLTRIAYEILEKNKGMENLVLVGIRT
                     GGVYLAERLQKKMSEIEHGKIPMGILDVTLYRDDISVSNKKPRLGETKINFSLDEKIV
                     ILVDDVLFTGRTIRAAMDALIDFGRPRMIQLAVLIDRGHRELPIRADFVGKNLPSSLW
                     EAVRVDLVEKNGVDEVVIEEGT"
     gene            1396..2322
                     /locus_tag="PLG98_10960"
     CDS             1396..2322
                     /locus_tag="PLG98_10960"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOE33826.1"
                     /translation="MKFERKDILGIKDLSVDEINLILETAGSFLEVSTRKIKKVPTLR
                     GKTVINLFYEASTRTRTSFEIAGKRLSADTINISSSTSSVMKGETLIDTARNLEAMNP
                     DVIVIRHSAAGAPHLLASLVKQSIINAGDGAHEHPSQALLDMMTIKERKGNFPGLKVA
                     IIGDIEHSRVVRSNIYGLSKMGATVVVAGPATMLPRDIEQMGVKVFDKMEDAITDADV
                     VMMLRIQLERQKQNIFPSLREYSQRYCLNRNNIKLAKKDVIVMHPGPINRGVELSPDI
                     ADDPSFSVILDQVNKGVAVRMALLYLLTGGSE"
     gene            2319..3617
                     /locus_tag="PLG98_10965"
     CDS             2319..3617
                     /locus_tag="PLG98_10965"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOE33827.1"
                     /translation="MKLLLTGARVIDPAQKIDSRLDVFLENGKIARVGANLSKSTKSK
                     DAEKITKIIDLAGMIIVPGLIDMHTHLREPGFEYKETVASGAAAAVAGGFTSIACMPN
                     TNPVNDNRSITEFIKAKAAEANLANVYPIGAISRNSDGDQLTEFWDLKEAGVIALSDD
                     GKPVMNAALMRRAMEYAHSLQLPVINHCEDIHLSANGAMNESYYSTILGLNGIPAIAE
                     EVMVARDILIAEYTGTSVHIAHVSTAGSVRLIRSAKERGVNVTAETAPHFFTLTDEAL
                     QGYDTFFKVNPPLRGEKDVKAIKEGLADGTIDVIACDHAPHARTDKEVEFEYAANGIS
                     GLETSLSLSLNLIHSKILSWPELVSRMSTRPARILNLPKGTLKEGADADITVIDPLLP
                     WTVDVQAFRSRGKNSPFHGRQMQGKALMTIVGGEIKYDGR"
     gene            3604..4380
                     /gene="recO"
                     /locus_tag="PLG98_10970"
     CDS             3604..4380
                     /gene="recO"
                     /locus_tag="PLG98_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOE33828.1"
                     /translation="MTAVREHFKTTGFVLRTLSYSESDLIVSFYSHDFGKLKGIAKGA
                     KRSKKRFANVFEPFSLTDIIFTRKSRDTLAFIESCDIVDHYDAIRANMEKTLIASYFI
                     DLTDHFSPEGKKNEDIFRLLQDFLVLLGREKVSDSIVRFFEIRLLKLAGFEPALDHCV
                     LCRTPVTNGNAYFFNAGRGGITCSACARTQKNEQPISAGTLRTLLLGKDMDIDKIKLV
                     TLTDSSATESRCLLTGFISHVLGREVKSWKVLEQVRRYCT"
BASE COUNT         1202 a         1183 c         1142 g         1010 t
ORIGIN      
        1 atcattgcca ttctgatcgc tttgtttatc agaacattta tcatatgcgc ctataagata
       61 ccgtcccgtt ccatggtgcc gacactgctg gtcggcgacc atattctggt caataaattc
      121 atctacggcg tgaaaatccc gctgttacga agaaccatta ttcccgtcag cgaacccaaa
      181 agaggcgaag ttgtcgtttt tatttatccc aacgaccggt ccaaagattt catcaaacgg
      241 gtgatcggtg ttgccggcga caaaattgaa ataaaaaaca agaaaatatt catcaatgac
      301 gaggagttca aggattccta cggcattttc agcgatagcc tcgttttacc gcaatccctg
      361 cagccgcggg acaatttcgg accgatcatc gttcccgaga aatccatttt cgtcatgggc
      421 gacaaccgcg atgaaagcct ggacagccgt ttctggggat ttgtcaacct gaaagacgtc
      481 gaagggaaag cctttgtcat ttactggtcc tggaaccgcg atgagaacaa tcttcgctgg
      541 aaccggctgg gtcaattgct gcactaggcc gttctccgga aggtttcggc gctttccgcc
      601 gtttgtcatt gcgtcatatc agcagacatt cgggaaatta ttttaaaatc ttgacacaca
      661 aacggtttgg ttatataacc atccccaata aagcctttta atttaatccg gagagattga
      721 aaaaggaaac gttgatgaga aaatcaaaga tccatgaaga ttatgaagcc tttaccgttt
      781 tggggaaagg cttttttttt aaccgggcaa ggggttagcg cacatgtcga atacgaaaag
      841 cacaaaaacc gtcatggata aggaaggcat tgagcggtgc ctgaccagaa tcgcctatga
      901 aattctggaa aagaacaagg gaatggaaaa ccttgtcctg gtcggcataa gaacgggcgg
      961 cgtgtatctc gccgaacggc tgcaaaagaa aatgtccgaa atcgagcacg gcaagatccc
     1021 gatgggcata ctggatgtca ccctttatcg cgacgatatt tccgtgtcca acaaaaaacc
     1081 gaggctgggc gaaacgaaaa taaatttttc actggatgaa aaaatcgtca tccttgtgga
     1141 cgacgtgctc ttcaccggca gaaccatccg cgcggccatg gacgctctga ttgatttcgg
     1201 acgcccccgg atgattcaac tggcggttct cattgaccgc ggtcatcgcg agctgcccat
     1261 ccgggcggat ttcgtgggca aaaatctgcc ctcctcgctc tgggaagccg tccgggtcga
     1321 cctggtggaa aaaaacggcg tcgatgaagt cgtcattgaa gaaggaacat aaacaagaat
     1381 tttttaaggc cccctatgaa atttgaacga aaagatattt taggaataaa agatttatcg
     1441 gtcgatgaaa tcaacctgat tctggaaacg gccggatctt ttctggaggt gtcgacgcgc
     1501 aaaataaaga aagtgccgac cctgcgcggg aaaacggtca tcaatctgtt ttacgaagcc
     1561 agcacccgca cccgaacatc ctttgaaata gccggcaaac gattaagcgc cgacacgatc
     1621 aatatttcat cgtccaccag ctccgtcatg aagggagaaa cgctgatcga cacagcccgc
     1681 aacctggaag ccatgaatcc tgacgtgatt gttatccgtc acagcgccgc cggcgctccg
     1741 catcttctgg cgtcgctggt gaaacagtcg atcatcaatg ccggcgacgg cgcacatgaa
     1801 catccttcac aggccctgct ggacatgatg accatcaaag aacgcaaagg aaatttcccc
     1861 ggattgaaag tggccatcat cggcgatatt gaacacagcc gggtggtccg gtccaacatc
     1921 tacgggctct ccaaaatggg cgccaccgtc gttgtggccg gtccggccac catgctgccc
     1981 cgcgatattg agcaaatggg ggtcaaggtc tttgataaaa tggaagacgc catcaccgat
     2041 gccgatgtgg tcatgatgct gcgcattcaa ctggagcggc agaagcaaaa catctttccg
     2101 tctctgcgtg aatattccca gcgctactgc ctgaaccgca acaacatcaa actggcgaaa
     2161 aaagatgtta ttgtcatgca tccgggaccg atcaaccgcg gtgtcgagtt atcccccgac
     2221 attgccgacg atccctcttt ctctgtaatt ctcgatcagg taaacaaagg cgttgccgta
     2281 cggatggcgc tgctctacct gctcaccgga ggtagcgaat gaaactctta ttgaccggcg
     2341 cccgggtcat cgatccggcg caaaagatag attcccgcct ggacgttttt ctggaaaacg
     2401 ggaaaatcgc ccgggtcggc gccaatcttt ccaaatccac aaaatccaaa gacgcggaaa
     2461 agataacaaa gattatcgat ctggccggaa tgatcattgt acccggcctg attgacatgc
     2521 acacgcattt acgcgaacct ggcttcgaat acaaggaaac cgtggcttcg ggtgctgccg
     2581 ccgcggtggc cggaggcttt acgtctatcg cctgcatgcc caataccaac cccgtcaatg
     2641 ataaccgctc cattacggaa ttcatcaagg caaaggcggc agaagccaac ctcgccaacg
     2701 tctatcccat cggcgccatc agcagaaatt cagacgggga tcagctgacg gaattctggg
     2761 acctcaagga agcgggcgtg attgcacttt ccgacgacgg caaaccggtc atgaatgccg
     2821 ctctgatgcg ccgggccatg gaatacgcgc attcgctgca attacccgtt attaatcact
     2881 gtgaagacat ccatctttcc gcaaacggag ccatgaatga aagttattat tcgacgattt
     2941 taggattaaa cggcatcccc gccatcgccg aagaagtgat ggtggcgcgg gatattttga
     3001 ttgccgaata taccggaacc tccgtgcata tcgcgcatgt cagtaccgca ggttccgtcc
     3061 gtttgattcg cagtgccaag gaaagaggtg taaacgtcac cgcggaaaca gccccgcact
     3121 ttttcacgtt aaccgatgag gccctgcagg gctacgatac gttttttaaa gtgaatcccc
     3181 cgttaagggg cgaaaaggat gtcaaagcca tcaaggaagg actggccgac ggaacgattg
     3241 atgtcattgc ctgcgatcac gctccgcacg cgcgcaccga taaagaagtg gaattcgaat
     3301 atgccgccaa cggcatcagc ggattggaaa catcgctgtc gctgagtctg aacctgatcc
     3361 acagcaaaat cctgagctgg ccggaactgg tttccaggat gagcacccgg ccggcgcgta
     3421 tcctgaacct gccgaaagga acgctgaaag aaggcgccga tgcagacatc acggtcattg
     3481 atcccctgct gccgtggaca gtggatgtgc aggcgttccg gtcgcggggt aagaattctc
     3541 ctttccacgg acgtcagatg cagggcaaag ccctcatgac catcgtcggc ggtgaaatca
     3601 agtatgacgg ccgttaggga acattttaaa actaccgggt ttgtcctgcg caccctgagt
     3661 tacagcgaat cggatttaat tgtcagcttc tacagtcatg acttcggcaa gctcaaggga
     3721 atcgccaaag gggccaagag aagcaaaaaa agatttgcca acgtctttga accgttttcc
     3781 ctgaccgata tcatttttac ccgaaaaagc cgcgataccc tcgcgttcat cgaatcgtgc
     3841 gacatcgtcg accactacga cgccatccgc gccaatatgg agaaaacgct tatcgcgtct
     3901 tacttcatcg atctcaccga ccacttcagc ccggaaggga aaaagaatga agatatattc
     3961 cgattgcttc aggattttct cgtcctgctg ggcagggaaa aggtctccga ttcgattgtc
     4021 cgtttttttg aaatccgtct gctgaaactg gccggtttcg aaccggcgct cgatcactgc
     4081 gttctttgca ggacgccggt gaccaacggc aatgcctatt ttttcaacgc cggccgcggc
     4141 ggcatcacct gcagcgcctg cgcgagaacg caaaaaaatg agcagccgat ttccgcaggg
     4201 accttgcgca ccctgctttt gggaaaagac atggacatcg ataaaatcaa actggtcacc
     4261 ctgaccgact cgtcagctac ggaatcacgc tgcctgctca cgggtttcat atcccatgtg
     4321 ctcggacggg aagtgaaatc gtggaaagtc ctggaacagg ttcgtcgata ctgcacatga
     4381 tggaaaatta aaatgcattc tccggaatta ttgattcccg cggggaatct ggaaaaattg
     4441 cgcacagccc tgctctacgg agccgatgcc gtttatgtcg gcgtggaagg attgagcctg
     4501 cgggcaagtt catccgaaat gtctctgacc gatttag
//
LOCUS       DAOSME010000118         4505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_20933_length_4505_cov_24.470562, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000118 DAOSME010000000
VERSION     DAOSME010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20933_length_4505_cov_24.470562"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..2134
                     /locus_tag="PLG98_10975"
     CDS             68..2134
                     /locus_tag="PLG98_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOE33829.1"
                     /translation="MKTNRLINEKSPYLLQHAKNPVDWYPWGKEAFEKARREDKPVFL
                     SIGYSTCHWCHVMERESFEDDDVAGLLNETFVNIKVDREERPDIDAVYMTVCQVMTQS
                     GGWPLTIMMTPDQEPFFAATFIPRTTRYGRAGMLQIVPRIKEVWKTKRADVLSAASEI
                     TGILNQSATDKGNRLSEETLRQANRSLSMRFDKTFGGFGAAPKFPSPHHFFFLLRYWK
                     RTGDADALHMAEHTLRNMRSGGIYDHVGFGFHRYSTDERWVVPHFEKMLYDQALMAMA
                     FIELFQATGKAEYEATVREIFTYVLRDLTDEQGGFYCAEDADSEGVEGKFYLWTEKEI
                     RGVLNEKEADLFLSLYRHPEDGDLPGMHEIPAGCFIPHLTPSAGDKKDLSGLLFSMEY
                     IREKLFSAREKRVHPHKDDKILADWNGLMIAALARGAQVLDEPVYAKAACRAMDFILE
                     TMQTGDGKLLHRYRSGEAAIDANVDDYAFVIRALLELYEATFQTQYLKKALEYNDDLF
                     SNFLDQKNGGLFFTSLKAERLLTRPKELYDGAVPSGNSVAYGNVLKMFRMTGNADMEA
                     KADAICKAFEAPAGAMPEAFTQFLCGLEFAAGPSGQVIITGDLGKPDTREMLRTLRKQ
                     FAPNNVVIFRPEKPVQPDIETIAPFVKAHLPLNGKATAFVCTDFACHLPTNDPQKMLE
                     LLDTRK"
     gene            2250..2639
                     /locus_tag="PLG98_10980"
     CDS             2250..2639
                     /locus_tag="PLG98_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33830.1"
                     /translation="MKKWLISIMVIAFTLSLAGCNFPNFIKKQSPLQEQCNKQCEAWS
                     KSYMQKYPSDKLTYESHYNKRLNKCFMLVTYSKSQLKSLKEISENKMYGSLLVKQNSK
                     TLICNVLENKCKTEKEWDALVKPYMEE"
     gene            2780..3817
                     /locus_tag="PLG98_10985"
     CDS             2780..3817
                     /locus_tag="PLG98_10985"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOE33831.1"
                     /translation="MNGLDFLGSMVDEMFLAPGRFIAWLQARRKTDWPAFAREIAFYH
                     EQGFVKNPSSFFVLPDQAPEFVVEKQASYRKGFYQVITFPSRYEPRNPFLRKRYLNHP
                     ENCTGYLIRWIHGDKPRKTVLCVHGFMMGNPREAEQMFKVRKLFSMGLDVALFIHPFH
                     WKRIAGPRASRRVYLTLGDAAFTNECIAHSIHDLHNSFLILHQLGVQDIGLVGASLGG
                     YVTGLYVCLSSLPEFAALMVPAVTFLRPISPALFYNQAPYGPQWRKQVYHAADFHSPL
                     HFYPKIPAEKILVVASRGDRICPADPVEQLRIKWGLSRCHYRTGGHWLVFDKIRGRVW
                     YGLLRDMGFIA"
     gene            4008..4268
                     /locus_tag="PLG98_10990"
     CDS             4008..4268
                     /locus_tag="PLG98_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOE33832.1"
                     /translation="MRVFILILCLFAAFLTFANAAELYRCVDQHGNAIVTDRPQAGME
                     NCITNDVDEEDSPADNKTDEENASADRPENNQEASDHQAAEP"
BASE COUNT         1174 a         1086 c         1172 g         1073 t
ORIGIN      
        1 aaagacgcga ttgaaaaagc attttccgcg gcgctgaaat aaatcgaagc aagtttctgg
       61 cacaaaaatg aaaaccaacc gcctgatcaa cgaaaaaagt ccttatctgc tgcagcacgc
      121 aaaaaatccg gtggactggt acccgtgggg aaaagaagca tttgaaaaag cccggcggga
      181 agacaagccg gtttttcttt ccatcggcta ttccacctgc cactggtgtc atgtcatgga
      241 acgagaatct ttcgaggatg acgacgtcgc cgggctgctg aacgagacat ttgtgaatat
      301 caaggttgac cgggaagagc gtcccgacat tgacgcggtc tacatgaccg tctgccaggt
      361 catgacgcaa agcggcggtt ggcccctgac gatcatgatg acgccggatc aggaaccgtt
      421 ttttgccgca accttcattc ccagaacaac cagatacggg cgcgccggaa tgcttcagat
      481 cgttcccaga ataaaagagg tctggaaaac aaaacgcgcc gatgttcttt ccgccgcgtc
      541 ggaaattacc ggcatcctga atcaatccgc caccgacaag ggcaatcggc tgtcggaaga
      601 aacgctgcgg caggctaacc ggtctttgtc tatgcgtttt gacaaaacat tcggcggctt
      661 cggcgcggct ccgaaatttc cctcgccgca tcatttcttt tttctgctgc gctactggaa
      721 gcgcaccggc gacgcagacg cgctgcatat ggccgagcat acgctccgaa acatgaggag
      781 cggcgggatt tacgatcatg tcgggttcgg ttttcatcgc tattcgaccg acgaacgctg
      841 ggtggtgccg cattttgaaa agatgctcta cgatcaggcg ctgatggcca tggcctttat
      901 cgaactgttt caggcgacgg gaaaagcgga atatgaagca acggtccgcg agatttttac
      961 ctacgtctta cgtgatttga cggatgaaca gggcgggttt tactgcgctg aagacgccga
     1021 cagcgaaggc gtagaaggaa aattttatct gtggacggaa aaggaaatcc gcggcgtcct
     1081 caacgaaaaa gaagccgact tattcctgtc actttatcgc catcctgaag acggcgattt
     1141 gccgggcatg catgaaattc ccgccggttg ttttatcccc catctgacgc cgtccgccgg
     1201 ggataaaaag gatttatcgg gattgctttt ttcaatggag tacatccgcg aaaaactttt
     1261 ttccgcgcgg gaaaaacgcg ttcatccgca caaggatgac aaaatattag cggactggaa
     1321 cggtctgatg attgcggcgc tggcgcgcgg cgcacaggtc ttggacgaac ccgtttacgc
     1381 taaagccgcc tgccgggcga tggatttcat tctggaaacc atgcagaccg gggacgggaa
     1441 actcctgcac cgttaccgca gcggcgaggc cgcgattgat gccaatgtag acgactacgc
     1501 tttcgtcatc cgggcgttgc tggaacttta tgaggcgacg tttcagacgc aatatctgaa
     1561 aaaagctctt gagtataacg atgatctgtt tagtaatttt ctcgatcaaa agaacggcgg
     1621 attatttttt acttccctga aggcggaaag gcttttgacc cggccgaagg aactttatga
     1681 cggcgccgtc ccgtccggga attccgtggc ctacggcaat gtactgaaaa tgttccggat
     1741 gaccggcaat gccgatatgg aggcaaaggc ggatgcaatt tgtaaagctt ttgaagcccc
     1801 ggcgggtgcg atgcctgagg ccttcacaca atttctctgc ggtctggagt ttgcggcggg
     1861 accttccggc caggtcatca ttaccggaga tttgggaaaa ccggataccc gggaaatgct
     1921 gcggactttg agaaaacaat ttgccccgaa caatgtcgtt attttccggc cggaaaaacc
     1981 ggtgcaaccg gatattgaaa ccatcgcgcc gtttgtgaaa gcccatttac cgctcaacgg
     2041 gaaagcaacc gctttcgtct gcaccgactt cgcctgccat ctgccgacga acgatccgca
     2101 aaaaatgctt gaacttttgg atacgagaaa atagtattgt tttgtatttt ccgtttgccg
     2161 gcgcttctca aaaccaagcg ttaaaaaagc gcctcaaagc agagcaatcc gaaattccat
     2221 gccggtcacg gttcttcagg aggcacatca tgaaaaaatg gttgatatct attatggtca
     2281 ttgcgtttac actatcactg gccgggtgca attttcccaa tttcataaaa aaacaatctc
     2341 cgttacagga gcaatgtaac aaacagtgcg aggcgtggtc aaaatcttac atgcagaaat
     2401 acccgagcga caaattaacc tatgaaagcc attacaataa gcgattgaat aaatgtttta
     2461 tgctggtaac ctactcaaaa tctcagttga aaagtttgaa agagatcagt gagaataaaa
     2521 tgtacgggtc cctccttgtc aaacagaaca gtaaaacgct tatttgcaac gttctggaaa
     2581 ataaatgtaa aacggaaaag gaatgggacg cgcttgtaaa accttatatg gaagaatagt
     2641 cgtgcaacaa aaaacggcat gataaaaaaa acgattttaa acaacgacct ccttttgcca
     2701 tcgtatttcc gtcgacacaa catcatgatt gatatttcat tgacagggga aattcttgtt
     2761 tatatattcg cgtatgcgaa tgaacggact ggatttttta ggcagtatgg ttgacgagat
     2821 gtttctggcg ccgggccggt ttattgcgtg gctgcaggct cgccggaaaa cagactggcc
     2881 ggccttcgcc cgtgaaattg ccttttatca cgagcaggga ttcgtcaaaa atccttcctc
     2941 tttctttgtg ttgcctgatc aggcgccgga gttcgtcgtt gaaaaacagg cgtcttatcg
     3001 aaaaggtttt tatcaggtca ttacttttcc cagccgatat gaaccccgga atcctttttt
     3061 acgaaaacgc tacctcaatc atcctgaaaa ctgtaccggc tatctgattc gctggataca
     3121 cggcgacaaa ccccgcaaga cggtattgtg cgtgcacggt tttatgatgg gcaatccgcg
     3181 ggaagcggag cagatgttca aggtccgcaa attattttct atgggccttg atgttgcgct
     3241 ctttattcat ccttttcact ggaagcgaat tgcaggcccc cgcgcgtcga gacgcgttta
     3301 tctgacgctg ggcgacgccg cttttaccaa cgaatgtatc gcacacagca ttcatgatct
     3361 gcacaattcc tttctcattc tgcatcaact gggtgttcag gacatcggcc tggtcggcgc
     3421 cagtctgggc gggtatgtca cgggattgta tgtctgtttg agcagtctgc ctgaatttgc
     3481 agccctgatg gtgccggcgg tgacgtttct gcgacccatt tctccggccc tgttttataa
     3541 tcaggcgccg tacggtccac agtggcgcaa acaggtttat cacgccgccg attttcattc
     3601 gccgcttcat ttttatccga agatcccggc tgaaaaaata ctggtagtgg catcgcgcgg
     3661 cgaccggatt tgtcccgccg atccggtgga acaactgaga attaaatggg gcttgtcgcg
     3721 ctgtcattac cggaccggcg ggcactggct ggtcttcgac aaaatccgcg gccgggtctg
     3781 gtacggtctg ctccgggaca tgggatttat tgcctgaaca acgagccggt tttgccggct
     3841 atgaatcagg ccaataaagg ggcttgtttt tcttccggat aagaattaaa aacaaagcat
     3901 caccagagaa gacgaagggg aagatacttc ccgaaaaagg atcttacgga gaaaatccgg
     3961 ctgtgaaatg gtacgttacc gttgcccgga tccggaagga gtgtggcatg cgagtattca
     4021 ttttaatcct gtgtttgttc gcggctttcc tgaccttcgc caacgccgca gagctgtaca
     4081 gatgcgttga ccaacacggc aacgccatcg tcacagaccg cccgcaggct ggaatggaaa
     4141 actgcattac gaatgatgtt gatgaagaag attcgcctgc ggacaataaa acagacgagg
     4201 agaacgcctc ggcggaccgg ccggaaaata atcaggaagc gtcggatcat caggcagcag
     4261 aaccttgagg ccggtaaaat acgcagttgc tcagacagtg gataaaatca tgacgttatc
     4321 atcaaatgaa gtaaatcttt ttttccggag gccgatatga gtgaaaccgt gattagcgag
     4381 aaagaccgaa agatggcgca acgttgtgtc gaatgcccgg tgtgcaaaaa ggccagaaaa
     4441 gagcagcggg gattcgccta ctggtttgtt aaaaatgtcg aagacggcct gtgcccgaac
     4501 tgtgc
//
LOCUS       DAOSME010000119         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_21516_length_4412_cov_14.886160, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000119 DAOSME010000000
VERSION     DAOSME010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21516_length_4412_cov_14.886160"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLG98_10995"
     CDS             <1..1235
                     /locus_tag="PLG98_10995"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOE33833.1"
                     /translation="LRNYEKEMQALPNVERDLARLTRVSSVNAGIYTFLLQKHEEARI
                     AKASTISNIDIVDQAIAPTHPVKPKKSQNLLIGLLFGCMLGIGLVFFQEYLDDTIKDA
                     EEAKRVMGLPLLGVIPHINGRDPKQNGSIPKENPLITQSEPKSPIAESFRSLRTNLHF
                     TAINKQKKIMLFTSTFHGEGKSVITANEAVVISQTGARVLIIDCDLRRSSLHEKFSHS
                     KTPGLSEILTGDVTFEKAKHNSGIPGLDLITAGTTPPNPSELLGSEAMRQFLLTQREN
                     YDYILIDAPPVLAVTDAPVLTTISDIVLVVIEAGRVPVKAAQHMREMLANVQAPVAGF
                     VMNDKTGRGETYGYYGSRYYRYGGYGRGYGRGYGYGYGYGYYSDEEQKPPSKKRWKKL
                     VNLLPEKLRTQIRNRLRL"
     gene            1339..1689
                     /locus_tag="PLG98_11000"
     CDS             1339..1689
                     /locus_tag="PLG98_11000"
                     /inference="COORDINATES: protein motif:HMM:NF008912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOE33834.1"
                     /translation="MGYRSFEELDVWKLGCNLAVRIYEALKDCKDFGLKDQMTRAAIS
                     ISSNIAEGAERNSKQEFIRFLHIAKGSAAELRTQTYIAYRIGIIDNSVQTDLTEELKK
                     ISAMLHSLIKSIKT"
     gene            1789..2490
                     /locus_tag="PLG98_11005"
     CDS             1789..2490
                     /locus_tag="PLG98_11005"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33835.1"
                     /translation="MIDFHSHILPGVDDGADDMNESVAMARALQAAGFETIHCTPHLI
                     KGCFDADNRMILSAVSSFQSRLNEDNIALEILPGREYYLDEFLDDHLMQPMPLGGTPF
                     IMIEIPNNAPEDFVKESCFKIKRSGFIPMIAHPERCRLFTAPAKNKTSRLWFSGGENK
                     NPEPDNETTALMDYLQDIGCAFQGNFGSFQGMYGPEVQHTANLLKKHNLFTHFGTDAH
                     SLKAVHFITEKNFER"
     gene            2812..4110
                     /locus_tag="PLG98_11010"
     CDS             2812..4110
                     /locus_tag="PLG98_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33836.1"
                     /translation="MKNIISQLIDDFHERKLPEPVPRNKSFSEVKGKADVVIGMRRTG
                     KTWFCYQKIKDLIAAGIKKEQILYLNFEDDRLLEFNVHNFQNIIDVYFGKYPEHRKTR
                     CYFFFDEIQRIDQWEMFIRRLLDTENIQIFITGSSSKLLGSEIATSLRGRSLAIEIFP
                     FSFEEYLKYHGHFFDRPLKYGAKTSSILRKAVKDYLEAGGFPEVQKTNRDLRIEILQG
                     YIDTVLLKDIIERHKVSNVLVLKYLVRHIMNSSGGQFSVNKFFNTMKSMSIKCTKNSL
                     YEYLDHLTDAFLFYKVPVHSRSEKSRLINPAKIYTIDTGLLNAMTFRHSSNYGHLLET
                     MVFMHLRRGAYDVEYINTKDGREVDFLTRHRISGKTQLIQVCWEISDKKTFERELRGL
                     KSAMDELSLPTGTIVTWDDETVIDKKINVMPVWKFLLTWD"
BASE COUNT         1326 a         1034 c          916 g         1136 t
ORIGIN      
        1 agcttcggaa ttacgaaaaa gaaatgcagg ctctgcccaa tgtcgaacgc gatcttgctc
       61 gtcttacgcg tgtttccagc gtcaatgcag gtatctacac cttcctacta cagaagcatg
      121 aggaagcgcg cattgccaag gcatccacca tcagcaacat tgacatcgtt gatcaggcta
      181 tagctcccac ccatccggta aagccgaaga aatcgcaaaa tctgcttatc gggttacttt
      241 tcggctgtat gctgggcatc ggtctggttt tctttcagga atatctcgac gataccatta
      301 aggacgccga ggaagcgaag cgggttatgg gtctgccttt gctcggagtc attccgcaca
      361 ttaacggccg ggatcccaaa caaaacggca gcattcctaa agagaatccg ctgataacgc
      421 agagcgagcc gaagtcgcct attgccgagt cgtttcgttc attgcgcacc aatttacatt
      481 ttactgccat caacaaacaa aagaagataa tgctttttac gagcacattt catggtgagg
      541 gaaagagcgt cattaccgcc aatgaagctg ttgtcatttc ccagacagga gcgcgcgttc
      601 tcattatcga ctgcgatttg cgccgctctt ccttgcatga aaaattcagc cacagcaaaa
      661 caccgggatt gagcgaaatt ttaaccggag acgtaacatt tgaaaaagca aaacataaca
      721 gcggtattcc cggccttgac cttatcactg ccggaacaac tccgcccaac ccttcggaac
      781 ttcttggttc agaggcgatg cgtcaatttc ttttaaccca gcgggaaaac tacgattaca
      841 ttttaataga cgctccgccg gttctggccg tcaccgatgc cccggttctt actacgatat
      901 cggacatagt gcttgtagtc atcgaggccg gccgcgtacc agtcaaggca gcccagcaca
      961 tgcgcgagat gctcgccaac gtccaggccc ccgtagctgg tttcgttatg aacgacaaaa
     1021 ccggtcgcgg cgagacttac ggctactatg gcagccggta ttacagatac ggtggatacg
     1081 gacggggata cggacgcggc tacggatacg gttacggata cggctactat tccgacgaag
     1141 aacaaaaacc accctcaaaa aaacgctgga aaaaactcgt taacctcctc cctgaaaaac
     1201 tgagaacaca aatcagaaac aggttgagac tttaagttca aagtgaaaaa agaaagtttt
     1261 acgttttacg tgttaggtgt taagtaacaa caactgaaat ttattctact ataacgttta
     1321 ccctggatat tttgattgat gggatatcgg tctttcgaag aactcgacgt ttggaaatta
     1381 ggatgcaatc tggctgttcg gatttacgaa gcactaaaag attgtaagga ttttggcctg
     1441 aaggatcaga tgacaagagc ggccattagc atttccagca atatcgccga aggtgcggaa
     1501 cgaaattcca aacaggaatt tatcagattt ttacacatcg ctaaaggttc tgcagcagag
     1561 ctccggactc agacttatat agcctacaga attggcatta tcgataacag cgtccaaaca
     1621 gaccttacag aggagttaaa gaaaatatca gctatgcttc acagcttaat caaatctatt
     1681 aaaacataaa aacagtttta ggttttacgt gttacgtgtt acgttttacg taaaaataaa
     1741 caactatttc tattttacct aaaacctaaa acttaaaacg ttttttctat gatcgatttt
     1801 cactctcaca tactgcccgg ggttgatgac ggggcggacg acatgaatga atccgtcgcc
     1861 atggcgcggg cgcttcaagc tgccggattt gaaaccatcc actgcactcc gcatctgatc
     1921 aagggctgct tcgacgcgga caaccggatg attctttccg ccgtctcctc ctttcagtcc
     1981 cgactcaatg aagacaatat tgcgctcgaa atccttcccg gacgggaata ttatctggat
     2041 gaatttcttg atgatcatct catgcaaccg atgccgctgg gcggcacgcc atttattatg
     2101 atagaaattc ccaacaacgc gccggaagat tttgtgaaag aatcatgctt taaaataaaa
     2161 cgctccggct ttattccgat gatcgcgcat cctgaacgtt gccggctttt tactgcgcct
     2221 gccaaaaaca aaacatcacg cctctggttt tccggcggag aaaacaaaaa tcccgaaccg
     2281 gataatgaaa ccaccgcgtt gatggattac cttcaagaca tcggatgcgc atttcaggga
     2341 aacttcggca gttttcaggg catgtacgga ccggaggtgc agcacactgc gaatctattg
     2401 aagaagcaca acctcttcac ccatttcggc accgacgccc actccctgaa agccgttcac
     2461 ttcattacgg agaaaaactt tgaacggtga actctctatg tcactccgac tgccctttat
     2521 gccacgtcga tcgcccttta tgtcacttag accgcccttt atgtcatttc gacctaaggg
     2581 agaaatcttg aaacctcgaa taaaaccacc aataggtgat aattcgttct gcaagcttca
     2641 gtgcacttca tttctgaagt ttgggactca ttagatttct cgtcgctccg cttctcgaaa
     2701 tgacacaaaa agagtttgtc acttcgaacg catgcgagaa gtccttattt gctctaataa
     2761 agagataaaa aataattgca ttatctctaa taaagagata atattcccaa catgaaaaat
     2821 atcatttcac aacttattga cgattttcac gaaagaaagc tccctgaacc ggtgccgcga
     2881 aacaagtcat tttctgaagt taaggggaag gcggatgtcg tcatcggcat gagacgcact
     2941 ggaaagactt ggttttgtta tcaaaaaata aaagacctga ttgccgccgg aataaaaaaa
     3001 gaacaaatcc tctatcttaa ctttgaagat gaccggctgc tggagtttaa cgttcataat
     3061 ttccagaaca ttattgatgt ctatttcgga aaataccctg aacatcgaaa aacacgatgt
     3121 tactttttct ttgatgagat acagcgaatt gatcaatggg aaatgttcat ccggcgtttg
     3181 ctggatactg aaaatattca aatattcatt accggatcat catcaaaatt gcttggttcg
     3241 gaaatagcca ccagtttgcg cggacgttct ttagccatag aaatattccc gttcagcttt
     3301 gaggaatatt taaaatatca tgggcatttc tttgacagac ccctaaaata cggtgcaaaa
     3361 acatcttcca tattacgcaa agccgtaaaa gactatcttg aagcaggagg cttcccggaa
     3421 gtacaaaaaa ccaatcgcga cctgcgtatc gaaatattac aaggttatat tgataccgtg
     3481 ttgctgaaag acattatcga acgccataag gtgagcaatg ttctggtgct caaatatctt
     3541 gtccgccata ttatgaattc ttccggcggg caattcagtg tcaacaaatt cttcaatact
     3601 atgaaaagca tgtcgatcaa atgcaccaag aacagcctgt acgaatacct tgatcatttg
     3661 acagacgcct ttctgtttta caaagttccg gttcacagtc gttctgaaaa atcaaggctc
     3721 ataaaccctg ccaaaatcta caccattgac accggacttt taaacgccat gacctttcgt
     3781 cattcatcta attacggcca cctgctggaa accatggtct ttatgcacct gcggcgcggg
     3841 gcgtatgacg tcgaatacat caacacaaaa gacgggcgag aagtcgattt tcttacccgg
     3901 cacagaattt ccggcaagac gcaacttatt caggtctgct gggagatatc ggataaaaaa
     3961 acctttgaaa gagaactccg cgggttgaaa agtgcaatgg atgaactttc actcccgaca
     4021 ggtacaatag taacctggga tgatgaaacc gtcatagaca aaaaaatcaa cgtaatgcct
     4081 gtctggaaat ttcttctaac ctgggattga gaacatcgaa ccttcaactt tttaagagcg
     4141 tagcgaatgg gacaatggaa caagatttct cgtcgctccg cttctcgaaa tgacacaagg
     4201 tgtgaatgtc aaaattcttt tgtcacttcg aacgaacgtg agaagtcttg tcttttactt
     4261 aatacctaaa acttaacacg taaaactgct tttttaacct tgaactattt tttatcgaaa
     4321 ggaacttatg aaaaatcaca aacaaacctt attaacaaaa ataaatgaag gcaaagccct
     4381 gatcggcatc atcggcctgg gatacgtcgg cc
//
LOCUS       DAOSME010000120         4395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_21642_length_4395_cov_15.268894, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000120 DAOSME010000000
VERSION     DAOSME010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21642_length_4395_cov_15.268894"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLG98_11015"
     CDS             complement(<1..201)
                     /locus_tag="PLG98_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE33837.1"
                     /translation="MAAAACRRFSKNRRKQENRRGTQGTTSRSLHLPASQERFMSIIE
                     IDQDKCIGCGTCRADCPTANIAI"
     gene            complement(502..1509)
                     /locus_tag="PLG98_11020"
     CDS             complement(502..1509)
                     /locus_tag="PLG98_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOE33838.1"
                     /translation="MPDIPILLVIIIVVALVFDYTNGAHDSANAIASVVSTKVLTPKA
                     AVIMAGVLNFTGAFLGTHVAETIGKGIVRPEMIMGCHTLLLAALAGAICWNFLSLYIG
                     LPSSSSHALIGGLMGSGIVYSGWSSLEYGSILEKVIIPLVLSPLVGFIAGYIIMLGLA
                     WLTCRFRYQSTNSVFKKLQLIAAAFMALSHGMNDAQKTMGIIALALVIFHQIPTLYVP
                     FWVKVACASVIMLGTLNGGWKIIKTMGQKIFKMQPVHGFAAQSATAGVILSASLFGAP
                     ISTTQVISSSILGVGSSKRFSAVRWGVAGNMVMAWFVTIPAAALVAGLCMFLFKLIGL
                     A"
     gene            complement(1502..2131)
                     /locus_tag="PLG98_11025"
     CDS             complement(1502..2131)
                     /locus_tag="PLG98_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOE33839.1"
                     /translation="MSISFFPKIVKFFDLFEKQSGTLKDAAVVLDSIFQDFNEVTSKC
                     DRINKLETRGDSLSREISTELAMTFITPLDREDIYAINMAQEETLNMIKAVSSRIGLY
                     KFKTLEQSAVDLVEYLRLIIEETEKMIRKLGSKKIVEEHSKTVHKIKNEAELQLLVAL
                     GELYDSHSASPDRNLYILMWTQIYDRIEQALEKAEFLANTIEGISIKNA"
     gene            complement(2443..3621)
                     /locus_tag="PLG98_11030"
     CDS             complement(2443..3621)
                     /locus_tag="PLG98_11030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE33840.1"
                     /translation="MEQAINEKKLWTKDFITIAVINAIVFLTHFLLIVTIASYAVDAF
                     RASTDMAGLVAGIFIIGALIGRLGTGRIIEGIGSKRVLIISTMLFIVTSALYFAAVSL
                     SLLILIRLLHGIVFGAASTATGTIVAQIIPPSRRGEGIGFYSIGAILAVAVGPFIGIF
                     LIHHADFRMIFVLTSLLAAASFALSFAVNKPVYEKPVVESAAADRRFRLSDFLEYKAI
                     PISLIILIIGFSYSGLLTLISLYAKEIRLEKAASFYFLVYSVAVLVTRPLSGRLMDIK
                     GANFVVYPCLLLYAAGMLVFSQATAGIALLAAAILIAMGYGNFFSVAHAASIRGIPPH
                     RMGLAITTFFIFVDLGFGIGPYALGALVAVTGYRGLYVMMTLVILASIPLYYFLVGRK
                     "
     gene            3789..4115
                     /locus_tag="PLG98_11035"
     CDS             3789..4115
                     /locus_tag="PLG98_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33841.1"
                     /translation="MTAMHNNWKIDETGITFPAADGFPLAGTLLLPDQPLTAVLISAA
                     TGYPRDFYLPFARYGSARGAACLVYDYRGVAASASKDLQSFKMDCPDWGRLDMAAALD
                     RLKPHC"
BASE COUNT         1127 a         1123 c         1145 g         1000 t
ORIGIN      
        1 gatggcgatg ttggcggtgg gacaatccgc ccggcaagtt ccgcagccga tgcacttatc
       61 ctgatcaatt tcgatgatgc tcatgaacct ctcctgtgat gccggaagat gaagacttct
      121 tgacgtcgtt ccctgtgttc cccggcgatt ttcctgcttc cgcctgtttt ttgaaaaccg
      181 ccggcaagca gccgccgcca cggcgatttc cggttggaaa taatatgggg cttttccctt
      241 gtcaagacaa aagaacgggg tgcttaattt tcagtatgga tgcgccatga cctttcattc
      301 cttcaggttc aattgtcacc tcgtatcggc tgtggcacga tcacgagaac agggttgggc
      361 aaaattccac tgcaggatga gctgcgtgac tgagaaatgc ggaaaaacaa tgttgtttgc
      421 cgggcagtga tcacgatatc attgggggag gatgaatgga aggacatctt tgggtgatga
      481 cggcagcgac tccgaaaaga atcaggcgag tccgatcagc ttgaacagga acatgcacag
      541 ccccgccacc agcgctgcgg ccggtatggt cacaaaccag gccatcacca tgtttcccgc
      601 cacgccccac cgaaccgcgg aaaacctttt tgaagaaccg acaccaagga tggaggatga
      661 aataacctgc gtggtgctga tgggggctcc aaacagcgac gcgctcagaa tgacgccggc
      721 cgttgcgctc tgggcggcaa agccgtggac aggctgcatt ttgaaaattt tctgtcccat
      781 ggtcttgatg attttccatc cgccgttaag tgtccccagc atgatgaccg acgcgcaggc
      841 aaccttaacc cagaacggaa catacagggt tggaatttga tgaaaaataa ccagcgccag
      901 ggcaataatg cccatcgttt tctgcgcgtc attcatgccg tggcttagcg ccatgaacgc
      961 ggcggcaatc aattgaagct ttttaaaaac ggaattggtt gattgataac gaaaccggca
     1021 ggtgagccac gcaaggccaa gcataatgat atatccagca atgaatccca ccaaagggga
     1081 tagaaccaaa gggataatta ctttttccag aatcgaacca tattccagcg acgaccatcc
     1141 actgtaaaca atgcctgagc ccatcagtcc gccgattaat gcgtgcgagg aggaagacgg
     1201 cagaccgatg tacaagctca ggaagttcca gcatattgcg ccggccagcg cggccagcag
     1261 cagcgtgtgg cagcccatga tcatctccgg ccggacaatg cccttgccga tggtctcggc
     1321 aacatgggtt cccagaaaag cgcccgtaaa attaagaacg ccggccatga tcaccgctgc
     1381 tttcggcgtg aggacttttg tggagaccac cgatgcgatg gcattcgcgc tgtcatgggc
     1441 cccgttggtg taatcaaaga ccagcgccac cacgataatg atcacaagta aaatcgggat
     1501 gtcaggcatt ttttatgctg atcccttcaa tggtgttggc caggaattcg gctttttcca
     1561 gcgcctgttc aatgcgatcg taaatctgcg tccacatcag aatatacaga tttcgatccg
     1621 gagatgccga atgcgaatca tagagttcac caagcgcgac aagcagttgc agctccgctt
     1681 cgtttttaat tttatgaacg gttttggaat gttcttcaac gatctttttg ctgccaagct
     1741 tccggatcat cttttccgtt tcctcgatga tgagcctcag atattcgacc agatcgacgg
     1801 cggattgctc cagcgtttta aatttgtaca gcccaatcct tgaggaaaca gccttgatca
     1861 tatttaaagt ctcttcctgc gccatgttaa tcgcgtagat gtcctcgcgg tcgaggggcg
     1921 tgataaatgt catggccagt tcggtggaaa tttctcttga aaggctgtcg cccctggttt
     1981 ccagtttatt gatacgatca catttggaag tcacttcgtt aaaatcttgg aagatggaat
     2041 ccaggacgac cgcggcgtcc tttaaagttc cgctttgttt ttcaaacaaa tcaaaaaatt
     2101 taacaatttt tgggaaaaag ctgatgctca tgtcaacctc cgcactccat ggaagccaga
     2161 aattaggatc atatctttac ggattgttgg gcaaatatcc ctttcgcttg cttaaaatcg
     2221 ttctggataa attgaaggct tgcatcaggc aatgacaaaa cgatttcatt ggccgctcaa
     2281 atggatatgg tttgggaaac aatctgttaa aacatccgat ttcagctttc cggttaccct
     2341 gatgtaatgt tcatgttgcg ctgcgaataa tataacccct taaataacgc aagaaaaaac
     2401 aggaaatgaa gcgagcttcc cctcgtgtgc gttcttgcct gatcattttc tccccacaag
     2461 gaaataatac aggggaatgg aggccagaat gacgagagtc atcatgacat acaggccgcg
     2521 gtaccccgta acggcaacca gcgcgcccag cgcatagggg ccgatcccga atcccagatc
     2581 cacaaagatg aagaaggtcg tgatcgcgag ccccatccgg tgaggaggaa tcccccggat
     2641 ggaagcagca tgcgcgacgg aaaaaaagtt tccgtagccc atcgcgataa gaatcgccgc
     2701 cgcgagcagc gctattcccg ccgtggcctg actgaagacc agcatcccgg cggcgtaaag
     2761 cagcagacag ggatagacga cgaaattggc gcctttgatg tccatcaggc gcccggacaa
     2821 cggccgcgtg acaagaaccg cgacggaata cacaagaaaa tagaagctgg cggctttttc
     2881 cagacggatt tccttggcgt acagggagat gagcgtcaac aggccggaat agctgaaacc
     2941 gataatcagg atgatcaggg agatcgggat cgctttatat tccagaaaat cggaaagacg
     3001 gaatcgcctg tccgccgctg cggattccac aacaggcttc tcatagaccg gtttgttgac
     3061 cgcgaaagac agcgcaaaac tggccgccgc caaaagcgag gtcaaaacga agatcatcct
     3121 gaaatccgcg tgatgaatca ggaagatgcc gataaaaggg cctaccgcca ccgctaaaat
     3181 tgcgccgatg ctgtaaaagc cgatgccttc tccacggcgg ctcggaggaa taatctgggc
     3241 gacaatggtt ccggtggccg tgctggccgc accgaagaca atcccgtgca gcagccggat
     3301 gaggatcagg agagacaggc tcacggccgc gaagtacagc gccgaagtga cgatgaaaag
     3361 catcgtgctg atgattaaaa ctctcttgct gccgatgcct tcaatgatgc gcccggttcc
     3421 caggcgtccg atcagcgcgc cgataatgaa gatgcccgcg acgagccccg ccatgtctgt
     3481 agaagcgcgg aatgcatcca cggcgtaaga cgcgatcgtg acgatcagga gaaaatgcgt
     3541 cagaaagaca atcgcattga tcacggctat tgtgataaaa tcctttgtcc acagtttttt
     3601 ttcgttgatg gcctgttcca cgaccgactg ctttcgtttt gtgtttgccc gggactatag
     3661 gagcataact tccgcatgtc aacaagaatg gatgaacctg cttttattct tgcattgttt
     3721 tttcaaagtt gtaagatggc gaataatttg aaaatcaatt cttcggaaag tctggagaat
     3781 ccgtttcaat gacagccatg cataacaact ggaagatcga cgaaaccggg ataacgtttc
     3841 ccgccgcaga tggatttccg cttgccggca cgcttttgct gccggatcag ccgctaacgg
     3901 cggtactcat ctccgctgcg accgggtatc ccagagattt ctatcttccc ttcgcgcgat
     3961 acggatcggc gcggggcgcg gcctgcctgg tgtacgacta tcgcggcgta gcggcttcgg
     4021 cgtcgaagga tctgcaatcg ttcaaaatgg actgcccgga ctggggcagg ctggacatgg
     4081 ccgcagccct cgaccggctg aaaccgcact gctaaatgac atcagggcgc cgatcgcctc
     4141 ttacgttttt tagcgacgat ccgctgacca ccgccgaaag cgcaaaaatt cctgacgtgc
     4201 atgcccaacg cccggacgga gattcatgcc cttgcggcct ttagtaaaat gagattctga
     4261 ccgatgtcga tgagtttggt tataaaaatt tcagatggat ttttccgctg gtgataaaga
     4321 ttccaggggg aggagatcag catgaatacc gatcagttat ttcttcgggg gagcagtctc
     4381 aaggccttca tcgtc
//
LOCUS       DAOSME010000121         4292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_22331_length_4292_cov_20.489261, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000121 DAOSME010000000
VERSION     DAOSME010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22331_length_4292_cov_20.489261"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1753)
                     /locus_tag="PLG98_11040"
     CDS             complement(284..1753)
                     /locus_tag="PLG98_11040"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOE33842.1"
                     /translation="MCEIGKSAYRGHEVIYPQPFGVITCAGEKKYPVDYSHFNIMGTI
                     TGAHGIEADSDKAIFPAVNLEVTIGHDKGIKFRLPWIISGIGSTNIAKNNWEGLAIGS
                     ALAGTGLTIGENVVGMDPEAKFKNGKIIDTVDLKRRVTLYRDYQSNGYGAIVVQANVE
                     DGRLKVHEYAIEKLGVECVELKWGQGAKDIGGEVKIKDLKKAQMLHDRGYIVLPNPYD
                     PNVIKAFEQGEFKEFERHSRVGMVTEESFASTIDRLRKTGAKYIFLKTGAYRPVDLAR
                     AIAFSSRYKLDLLTVDSAGGGTGMSPWRMMNEWGVPPVELHSLVYQYAKKLAEKKKYL
                     PAIAVNGGFTFEDQIFKGLAMGAPFVKMIGMARAPIAAAMVGKTIGLSIEAQQVPVYI
                     ERFGNSKEEIFVTASSLRQKLGDKDFEKLPTGAIGLYTYYERLAQGLRQLMAGSRKFS
                     LEHISRNDLAALTPEAARISGINYIMDVDKDEAFRVLDI"
     gene            complement(1890..2567)
                     /locus_tag="PLG98_11045"
     CDS             complement(1890..2567)
                     /locus_tag="PLG98_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOE33843.1"
                     /translation="MINLTINGLPVSVEKGTTILEAAKFYGFPIPTLCHKEGLSPYGA
                     CRLCVVEIGEGSAAKLVSSCTYPVEDGLKVRTASERVLKARRIIIELLLASCPQSKTI
                     QDIASRHNVRQQRFRQEYEDCILCGLCVRMCKEQMMAGAIGFRGRGQNRSIGTPFDIK
                     SEVCRLCGGCIEVCPACQTRCTYTEPEKAICGGCANLAPPCIEKEQFHDMMCYMSPCV
                     ACEIKKV"
     gene            complement(2560..>4292)
                     /locus_tag="PLG98_11050"
     CDS             complement(2560..>4292)
                     /locus_tag="PLG98_11050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE33844.1"
                     /translation="ILLEELAQTVKDTTMCGLGQTAANPVLSTLRYFRDEYERHINDK
                     RCDAFVCKELTGAPCQAACPVDTEPWHYVALVEKGEYEEAYRVIRSANPFPSVSSRVC
                     DRKCESRCNLGISGGEAVGIRAIKRFVTDRIDSSAYKPKLGRKKKQKVAVVGAGPAGL
                     TAAHCLSLLGYGVTVFEAEDRPGGMLSCSLPSYRLPKNIVEKEIKTILNKNIKMEYGR
                     ALGKDLTIQDLRQKFSAIFLSIGAHESWPLNLENEDVEGVFPSIQFLKAFNMRGEELA
                     RGHVGVIGGGNSAVDAARVALRQKEVISVSLLYRRTREEMPAYEEEIDAALEEGVRIE
                     TLISPVKIHVRQEEIEAAIREGVELETLVTPIKIYSKEGRVVGIECIKNRLGEIDASG
                     RRKPTPIPGSEFRLELDTLIVAIGEKPKSEFLAHMGLEIDKGGRIKINRKTLETSMKG
                     VFAGGDLVTGPNTVIDAIAAGKKAAQSIDRFLQNKPLTIPATPKLPSVFIEPTAVDET
                     AAQALRRVEPAVLPPSARIKNFREVTMALPEEQARAECRRCLRCDLAFTSEQTIGQPM
                     AAATGGVQHD"
BASE COUNT          961 a         1108 c         1062 g         1161 t
ORIGIN      
        1 aaattgtccg gttgtcgggt ttgcggcagg ctccaatgca gatgagttat ttatccggca
       61 aaaaggtttg ttggctggta tgactgacga agtattaagc ttttaccagg agaagagcga
      121 tctgctgtat ggcttagatc acacataaat aattgtttgc cacagattat tgtgaatcag
      181 attattgcat gtcaataaca aaagatgagg atataatcaa taaaggaaca gaaatcgatt
      241 ccccgtggct attgaaaccg gaggaatcga ttaccgcaaa attttatata tcgagaacac
      301 ggaatgcttc gtccttatca acatccataa tataattaat gccgctgatg cgcgctgcct
      361 cgggggtgag agcggccaga tcgttacgcg atatatgctc cagagaaaat tttctgctgc
      421 cggccataag ctggcggagt ccttgggcca atctttcata gtatgtatag agtccaatgg
      481 cgccggttgg cagtttttca aaatctttgt cgcccagttt ctggcgcagc gagcttgcgg
      541 tgacgaaaat ttcttccttg gaattgccga aacgttcgat gtaaactgga acctgctggg
      601 cctcaatgga aagtcctatc gttttcccga ccattgccgc cgcaatggga gcgcgcgcca
      661 tgccgatcat tttgacgaaa ggcgcgccca tcgccaggcc cttgaatatt tggtcttcaa
      721 atgtaaatcc gccgttcacg gcgatagcgg gcaggtattt tttcttttcc gccagctttt
      781 tggcgtattg atacacaagg gaatgtaact ccacgggagg gacgccccat tcgttcatca
      841 tccgccacgg gctcatccct gtgccgccgc ccgcgctgtc cacggtaagc aaatccagtt
      901 tgtatctgga ggaaaaagcg atggctctgg ccagatcaac gggacgataa gcgccagttt
      961 tgagaaagat atatttcgct ccggttttcc gcaagcgatc aatggttgag gcaaaagatt
     1021 cttccgttac catgccgaca cgcgagtgcc tttcaaactc tttgaattcc ccttgttcaa
     1081 aagctttaat gacattcggg tcataagggt tcggcaggac aatatatcca cgatcatgta
     1141 gcatctgagc tttctttaaa tccttgattt tgacttcgcc gccgatatct ttcgcgccct
     1201 gaccccactt aagctccacg cactcgacgc cgagcttttc gatggcatat tcatgcactt
     1261 tcaagcggcc gtcttccacg ttggcctgta cgacaatagc gccataaccg ttgctctggt
     1321 aatctctgta gagagtaacc cggcgtttta aatcaaccgt gtcgatgatt ttgccgttct
     1381 tgaatttggc ctcgggatcc atgccgacga cgttttcccc gatagtaagg ccggttcccg
     1441 ctagggcgga acctatggct agtccttccc agttattttt ggcgatgttg gtggagccga
     1501 tcccagaaat gatccacggg aggcggaatt ttatgccttt atcatggccg atggtcacct
     1561 caagattaac ggccggaaag atggctttgt cgctatcggc ttcaataccg tgtgcgccgg
     1621 tgatcgttcc cataatatta aagtgcgaat agtcgacagg atattttttc tcgccggcgc
     1681 aagttattac cccaaagggt tggggatata taacttcatg gccacggtaa gctgatttgc
     1741 ctatttcaca catgccgaca caaccgtcga tacatgttac gcacataccc gaagttgacg
     1801 ttattgactc ctcggttctg tttttggtaa gtgttgcgct tgaggtgttt attcttgaaa
     1861 aagacatata taaatactcc tttctgggtt taaacttttt ttatttcgca cgccacacag
     1921 ggtgacatgt aacacatcat atcgtggaac tgttccttct cgatgcaagg cggcgccagg
     1981 ttcgcgcaac caccgcagat agctttttcc ggttctgtat acgtgcagcg ggtctgacag
     2041 gcggggcaga cttctataca gccgccgcag agccggcaca cttcggattt aatgtcaaac
     2101 ggtgtaccaa tgctcctgtt ttgcccgcgg ccgcggaatc ctatggctcc ggccatcatt
     2161 tgttccttac acatccgcac gcataatccg cagagaatac agtcttcgta ctcctggcgg
     2221 aatcgctgct ggcgcacgtt atgccgcgat gcgatatcct ggatagtctt ggactgcgga
     2281 caggacgcga gcaaaagttc gatgattatt cttcttgcct tgaggacgcg ttcagaggcg
     2341 gttctcacct taagaccatc ttcaacggga taggtgcagg aggaaactag tttagcggca
     2401 gatccttcgc cgatttcgac cacgcagagc ctgcatgccc cgtaaggtga gagcccttct
     2461 ttatgacaca gcgtcgggat gggaaagccg taaaactttg ctgcctcgag aattgtagtg
     2521 cccttttcca ctgatacggg caaaccattt attgtcagat taatcatgtt gcacacctcc
     2581 cgtggcagca gccattggtt gtccgatcgt ttgctcgctg gtgaaagcga gatcacagcg
     2641 caggcaacgt ctgcattctg cacgtgcctg ctcttccggc aaggccatgg tgacttcacg
     2701 gaagttcttt attcttgctg aaggcggcag caccgcgggc tcgacgcgtc ttaatgcctg
     2761 ggccgccgtc tcgtcgactg cggttggttc gataaagaca ctaggtagct taggcgtggc
     2821 cggtattgtc aaaggcttgt tttgcaaaaa gcggtcaata ctttgtgcgg cttttttgcc
     2881 ggcggcgatg gcgtcaatca cggtgttggg accagtcacc aggtcaccac ccgcgaacac
     2941 gcctttcatg cttgtctcca gagtttttct gttgatcttg attctgccac ccttatcgat
     3001 ttccagaccc atgtgagcaa ggaattcgct ttttggtttt tcgccgatag ccactatcag
     3061 ggtgtccagt tccagtctga attcgcttcc gggaatcggg gtgggcttgc gacgacctga
     3121 ggcatctatc tcgccgagac ggtttttaat gcattcaatc cccaccacgc ggccttcttt
     3181 agaataaatc ttgatgggcg tcacaagagt ttccaactcg acgccttcac ggatagctgc
     3241 ctcaatttct tcctgacgga catggatttt gactggcgag attagggttt caattctcac
     3301 gccttcttca agggccgcgt caatttcttc ttcataagca ggcatttctt cacgtgtgcg
     3361 tcgatagaga agagacacac tgatgacctc tttctgccgc agcgccactc tggcggcatc
     3421 cactgccgaa ttccctccgc cgatgacgcc cacatgaccc cgcgcaagct cctcgcctct
     3481 catgttgaat gctttcaaaa actgtatgga agggaaaacg ccttcgacat cttcattttc
     3541 cagattcaga ggccagcttt catgcgcccc aatggacaga aatatcgcgc tgaatttttg
     3601 ccgcagatcc tggatagtca aatctttccc cagtgctctc ccgtattcca ttttgatgtt
     3661 tttgttgaga atggttttga tttctttctc cacgatgttt ttgggtagcc gatatgacgg
     3721 cagcgagcaa gacagcatgc cgcccggtct gtcttccgct tcaaagacgg taactccgta
     3781 tccgaggaga gaaagacaat gagccgcggt aagtcctgcc ggtccggcgc cgacgacggc
     3841 gactttttgt ttctttttcc ttcccaactt cggtttatag gcagaagaat cgattctgtc
     3901 ggtgacaaac cgtttgatgg cacggatgcc gacggcctct ccgccgctta tgcccagatt
     3961 gcagcggctt tcgcatttgc ggtcacatac ccgagagcta acggagggaa acggattggc
     4021 cgaacggatc acacggtaag cttcctcgta ttcgcctttt tccacaaggg caacatagtg
     4081 ccacggttca gtgtccacgg gacatgccgc ctgacagggg gctccggtga gttctttaca
     4141 gacgaaagca tcgcatcgtt tgtcgttgat atgccgttca tattcgtcac ggaaatatct
     4201 caacgtgctc aaaacgggat ttgccgcagt ttggccaaga ccgcacatgg tggtgtcctt
     4261 aacggtctgt gctaactcct ccagaagaat ca
//
LOCUS       DAOSME010000122         4254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_22574_length_4254_cov_18.843296, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000122 DAOSME010000000
VERSION     DAOSME010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22574_length_4254_cov_18.843296"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            442..1218
                     /locus_tag="PLG98_11055"
     CDS             442..1218
                     /locus_tag="PLG98_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33845.1"
                     /translation="MTWRPQKPVSEAPIVFVAGWVSAVSGWMPLLKVMASRRPVYYIE
                     TREKRSALIEKRKPKPDDFSIKRMAEDLISVCHQLKIATPTTTVVGSSLGATALLEAL
                     KGGHLTAGTAFLIAPNSDFKAPWYLQWILFLPAFLYHGIKYFVLWYLRTFMVDVKKEP
                     EQMMRYNDTLRVAHPQRFKLSARSAIYDHYEVWPNLSSVTIPVALAYAPTDTFHSSDN
                     IRLLADRLPKATVVSCPSNKYMHSAEVMQDIEAFVFKSAS"
     gene            complement(1371..1790)
                     /locus_tag="PLG98_11060"
     CDS             complement(1371..1790)
                     /locus_tag="PLG98_11060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOE33846.1"
                     /translation="MNQKDRETILKKAQNEPFAKKFQLTFVDLQKGYAKVAMVFTPDM
                     ENIFGMAHGGAIFSLMDAAFEIASNSHGTMAVALNMSINYLASPEKGATLTAEAREIN
                     RTKKTAAYDIRATDDSGKLLASCQALVYRLEKPLPFL"
     gene            complement(2395..3555)
                     /locus_tag="PLG98_11065"
     CDS             complement(2395..3555)
                     /locus_tag="PLG98_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOE33847.1"
                     /translation="MYRIDLLNKISEKGLSLFSDQYEYRTDIPDPDGILVRSKEMKDM
                     TLPLSLKAIARAGAGVNNIPIDKCSEKGIVVFNTPGANANGVKELVILGMLMACRNVV
                     GGIAWAKGLVGEGDKVPDLIEKGKSKFAGTEIMGKKLGVVGLGAIGTMVANTAEGLGM
                     EVWGYDPFLSIEAAWRLSRNTKKAASLDKLLAECDFITLHVPQNKDTKGFMNTEKFAV
                     MKKGAVLLNFARGGLVDTAALKKAIADGIVGCYVTDFPDEEVIKTDKVIPIPHLGAST
                     EESEENCAVMAAMQLMDFLENGNIKNSVNFPECALDKSGKKRITIANQNTPGMIEKIT
                     HLLADNKLNIGDMINKSRGNLAYNIIDLEGDISEDLINKITSIEGIIAVRVI"
BASE COUNT         1098 a          951 c         1001 g         1204 t
ORIGIN      
        1 gacctgcccc aagactttgt accacaagtc aagttagaaa tccgttattt tttgttgttc
       61 catttatata cgcttccggt gcaggggcgt tagttcaatg tactgtacag cctgaatgtg
      121 ttgtattgaa gcctccattg ttcagtcagc ttccgggcct cattactgac cctgcctttc
      181 ttacgcagat tataaatgga cataattaaa ttttgcggac aagaaaaatt gtattttagt
      241 aggtaacggc aagattcttc ctgaaaataa gtgtccgcta ttttcaatag acattcaacc
      301 ttccaatatg taataaatac aaatatcttc tccataaggg ggattaatgt gaaagactcc
      361 atagcggatc cggtggcatt accggattca gtttttagca ggttgtcagt ttgcgtgcag
      421 agcggtgtcc gccttcgtgt catgacctgg cgaccccaaa aacccgtgag tgaagctccc
      481 attgtttttg ttgcgggatg ggtttcagcc gtatccggat ggatgccctt gttgaaggta
      541 atggcttcca gaaggccggt ttattatatc gaaacacggg aaaagcgatc agcactcatt
      601 gaaaaaagga aaccgaagcc ggatgatttc agtatcaagc ggatggcgga agacctgatc
      661 agtgtttgtc accaactgaa aatcgccaca ccaaccacca ccgtcgtcgg cagttccctg
      721 ggggcgacag cactgttgga ggcgctaaaa ggggggcacc tgaccgcggg aacagcattc
      781 ctgattgcac ccaatagtga tttcaaagcc ccatggtatt tgcagtggat tctttttcta
      841 ccggcttttc tatatcatgg gattaagtat tttgttctct ggtatttaag gaccttcatg
      901 gtggatgtta aaaaagaacc cgaacagatg atgcgttata acgatacgct gcgggtagcc
      961 catccgcaga gatttaagct gtcagccaga tcagccattt atgatcatta cgaagtctgg
     1021 ccaaacttga gcagcgtgac catacctgtc gctttggctt atgcccctac ggatacgttt
     1081 cactcatcgg acaatatcag actcctggcg gacagattac ccaaggcaac cgtcgtttcc
     1141 tgcccgtcaa acaaatatat gcatagcgcg gaggtgatgc aggatattga ggcgtttgtc
     1201 ttcaaatcgg catcataact ctgggaaaaa atggacagat aataaggttt attgtgctca
     1261 atgaaggctt ataatgttaa ctttcaagcg gttatcattg atatttagtg atgcaaataa
     1321 gtatgacatt tttaggtaaa atcagcgacc acttcataaa taattgccgc ttataaaaac
     1381 ggcagcggct tttccagccg ataaaccagc gcctgacagg acgccagaag ctttcccgaa
     1441 tcgtcggttg cgcgtatatc gtaggcggcg gtttttttcg tcctgttaat ttctctggct
     1501 tcggcggtca gcgtagcgcc tttttccggc gatgccagat aattgatgct catgttaagg
     1561 gcaactgcca tggtgccgtg tgaattggag gctatttcaa aagccgcatc cataagcgaa
     1621 aaaatagcgc cgccgtgagc catcccgaaa atattttcca tgtccggtgt aaaaaccatt
     1681 gccacttttg cgtatccttt ttgcagatcg acgaacgtta actggaattt tttggcgaaa
     1741 ggttcgttct gcgctttttt taaaatagtc tccctgtctt tttgattcat gagtattact
     1801 cctgcatgtt tttctggaca attgatacgt tcttgtaacg ctataataaa aaggggggta
     1861 atctgtccag ctgaaaacaa aaacgctcaa ttttgatctt gcttttgtgg tacaggctgc
     1921 aatattccga ctactaccta tagtagtgca aaaatacgat taccaccaca tattgatttt
     1981 tttcttgctt tttcaaaaaa atattataaa ataactgaaa gtttgaaaga aaatcttttg
     2041 gcagagaaat tgaggtttcg gtttggaagg ttctttgaag caaacggttt ttagatggca
     2101 aaatgccaaa gtttgtttaa actggagagg gaccgcttct gacggggaat tttcgatgta
     2161 tcgtcgggga gatagcaaaa cagaacatga tcatatggca aacgccgaat gaagcggttg
     2221 caaatggtta accaaatttt aaacccttgc ctgaagggcg gatcgtaaaa gatccgccct
     2281 ttttctttcg ttaaatgttc aggcaagtta aaatccgcca ttgctgtatc gaaaaaaaag
     2341 tgtctgacga aggccgccag acactttttg atatgacacg tgacagatgt tttcttagat
     2401 gactctaaca gcgatgattc cttcaatgga cgtgatttta tttatcagat cttcgctgat
     2461 atcaccttcc aggtcgatga tgttgtacgc cagattccct ctgctcttgt tgatcatgtc
     2521 gccgatgttg agcttgttat cggccagcag atgcgtgatc ttctcaatca tgcccggcgt
     2581 attctgattg gcaatggtaa ttcttttctt gcccgatttg tccagggcgc actcagggaa
     2641 atttaccgag tttttgatgt tgccgttctc caggaaatcc atgagctgca tggcagccat
     2701 cacggcgcag ttttcttccg attcttcggt ggaggccccc agatgcggaa tggggatgac
     2761 tttgtctgtt ttgataactt cttcatcagg gaaatcggta acgtaacagc ccacaatacc
     2821 atcggcaatc gccttcttca gggccgctgt gtccacgagc ccgcctctgg caaaattcag
     2881 gaggacggcg ccttttttca tgacagcgaa cttttcggtg ttcataaatc ctttggtatc
     2941 tttgttttgc ggaacgtgca gcgtgatgaa atcgcactca gccagaagct tgtcgagact
     3001 ggctgccttt ttggtgttgc gggagagcct ccaggccgct tcaatggaga ggaacgggtc
     3061 atatccccag acttccatgc ccaagccttc ggcggtattg gcgaccatcg tgccgatagc
     3121 gcccagaccg accacgccca gtttcttgcc cataatttca gttccggcaa acttggattt
     3181 gcccttctcg ataagatcag gcactttgtc gccttcgcca accagacctt tagcccaggc
     3241 gattcccccg acaacattgc ggcaggccat cagcatgccc aggatgacca gttccttcac
     3301 gccgttggcg ttggcccccg gtgtattaaa aacgacaatg cctttctctg aacatttgtc
     3361 gatgggaata ttgttgacgc cggcgccggc gcgggcgatt gctttcaatg ataacggcag
     3421 ggtcatatct ttcatttctt tacttctcac caggatgccg tccggatcgg gaatatcagt
     3481 gcgatactcg tactgatctg aaaaaaggct taaccccttt tcagaaatct tgtttaacag
     3541 atcgatgcga tacataaata cctcctattc ctttctaatg agacccaaat ttcagaaacg
     3601 atgtccactg aaacttgtag gtcgaatttt tccgaggcga agcatggcgg gacattgagc
     3661 catcccgaac agcgtagatg atgaacctca aaatttgctt cggggttcat acccgtgaat
     3721 aaggagttct ggcaggaaaa ctcctgagct ttgtcgccat agtacaatac agattgaatt
     3781 tatctcatgg atacgaaacc atctgttttg caggacgagt atagaatggg tgcttttacg
     3841 tgtcaaggaa atcttaatga gatccattga gagcaaacga agtgatgctc gaaatgcgag
     3901 ggatactgtc caataaccgg agtttattgg acagtatccc ccggtgcata gcgccgtgga
     3961 aactgtgttt acggtaagga aatatgaatt ttaacgagac ccagatttca gaaacgaagt
     4021 gcaccgaaat ttgatggtcg aattatcccc gaggcgaagc cgagcgggat aaatgtgggt
     4081 ctaattcccc gccccctaga gcgcagttcc tttggatacc atgctgttgt cggcggggtt
     4141 gtttatcata tcaaatgagt cttggtagat gtaaaaaaag gttcccttag ggggaaccct
     4201 tatttttcct ggtgggctgt gcggggattg aacccgcggc caatggatta aaag
//
LOCUS       DAOSME010000123         4223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_22764_length_4223_cov_21.878839, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000123 DAOSME010000000
VERSION     DAOSME010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22764_length_4223_cov_21.878839"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLG98_11070"
     CDS             complement(<1..1115)
                     /locus_tag="PLG98_11070"
                     /inference="COORDINATES: protein motif:HMM:NF033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retention module-containing protein"
                     /protein_id="HOE33848.1"
                     /translation="MAEIKNKGAMPSMDEKGHQVTGKVAILYGTVKAISPDGTVRLLK
                     VNSPVFADDRIITGDDGSVSIVFDTFPPTQLDLGRVSDVVIDEDVYGPVSPDVVAEAS
                     AEQKSIQEALLAGDQPIELDPTAAGVEQSTGGGHSVFVVSPDWIHVTPESGAETRGIS
                     WGLPSTEKYVAAQEDNSPPTITVYTGNDDGNGGSGDGSGDGAPGNVIGSNDVVYESGL
                     STGSNATANTEFAAGSFIISDPDGLDDIQSVTINGTEIAINNLSGTVITGTNGQLTIT
                     DYNSSTGVGTYMYELTTPTTDAPGVETDVFILTTTDGKSTSGEAKITIEINDDVPHAI
                     ADTNSIMEDAVEAITGNVLTNDQSGADTPATFVNWDST"
     gene            1420..2109
                     /locus_tag="PLG98_11075"
     CDS             1420..2109
                     /locus_tag="PLG98_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HOE33849.1"
                     /translation="MAPPKSIYNSHKIKLVSFAIFILVGLIVTGSVFTREKFHLNEKV
                     LQKAYEKYGQDAKMRLIHWENSLKKDSIPEREKLETINSFFNQQMVFVNDTDLYGVKD
                     YWATPVEFISRGAGDCEDFAIAKYFSLKIMGVDEAKLRIAYVKALRQNIFHMVMLYYS
                     DPAAEPLVLDNLVDSIRSASERRDLLPIFTFNGAGLWLAHDRRQGKLAGKSSRLTAWN
                     DLLQRMAGTGI"
     gene            2128..4086
                     /locus_tag="PLG98_11080"
     CDS             2128..4086
                     /locus_tag="PLG98_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE33850.1"
                     /translation="MTLYRQLVIFTVSLFFILFAGTWFAKFESTRLFLTNQLESHAQD
                     TASSLGLAISQYPDDLVSVETMVNAIFDRGYYETIRFMDPHGKILLERNLKVVIEGVP
                     AWFINLVPLKVPEANAYVTAGWRQAGTIYVKSHAGYAYKSLWQDIKHTTLLFLFCGMF
                     ILIAGGFGLRMLLRPLVLVEQQAEALCRKEYEIQTKIPRTRELRRVVHVMNRMTEKVK
                     EMFSEQVTQAEGLRERAYNDSLTGIGNRRYFESQVAAYLDNKENDFKGILMLIRIHGL
                     EILNEQRGFQVADELLKKVAASLQEMSVNLAGSVLSRLSGSDFVLFLPDYPSSESDRI
                     SGEVAKKLSGLEATGITASENVSNVGTCAFDRSVTLGQLLAEADLALAAANQAGANTW
                     HVRTIMESTAKIPFGQQQWKDALEKVLQNRRIVLYSQPVVKAGDKNSILHLEIFSRIV
                     LDTGEIINAGIFIPIAERLKLISSLDRLIIEEVSKLDRKKLGADTIAVNLSPSSLQDE
                     SFIQWIRDTLLHLPENAPRIIFEFAEFGAVQNENLVRSFIAVIRECGHEMGLDHYGQS
                     CGNLRHLQSLRPDYVKIDRAYTGELKDEDSDSRFFISSICSVAHSIDIAVIAEGVETD
                     NQWQLLKELNVDAVQGYIINSSKPITKV"
BASE COUNT         1075 a          984 c         1002 g         1162 t
ORIGIN      
        1 gtgctgtccc aatttacaaa tgtcgccggc gtgtctgccc ctgactggtc gttggtaagc
       61 acattgcctg taatggcctc gacagcgtct tccatgatgc tgtttgtgtc ggcgatggca
      121 tgcggaacat cgtcgttgat ctctatggtg attttcgctt caccggacgt gcttttcccg
      181 tccgttgtcg tcaagataaa gacgtcggtc tccacgccag gggcgtccgt tgttggcgtg
      241 gtgagctcat acatgtaagt accgacgccc gtcgaactgt tataatcggt tatcgttaat
      301 tgaccattcg taccggttat gaccgtaccc gaaagattat taatggcaat ttccgtcccg
      361 ttgatcgtta cactttgaat atcgtccagt ccgtccggat ccgatatgat aaaagaaccg
      421 gcggcaaatt ccgtattggc cgtcgcattg gaaccggtag aaagaccgga ttcgtatacc
      481 acatcattgc tgccgatcac gttacccggc gctccgtcac ctgaaccgtc tccggatccg
      541 ccattgccgt cgtcattccc tgtataaacg gtgatggtcg gaggagaatt atcttcctga
      601 gcggcgacat atttttctgt cgacggcaag ccccatgaga ttcctcgtgt ttcagccccg
      661 ctttccggag taacatgtat ccagtccgga ctgacaacaa acacagaatg gccgccgccc
      721 gtactctgtt caacgcccgc ggctgtcgga tccagttcta tgggttgatc accggccagc
      781 agcgcttcct gaatggattt ctgctccgcg gacgcctctg cgaccacatc cggagaaaca
      841 ggcccataca catcttcatc gatcaccaca tcggacaccc ggccgagatc caattgtgta
      901 ggggggaagg tgtcgaaaac aattgagaca ctcccatcat ctccggtaat gatacggtca
      961 tctgcaaaaa caggactgtt gaccttcaac agccgaacgg taccgtcagg ggatatcgct
     1021 tttacggtcc cgtacagaat ggcaactttt cctgttacct gatgcccttt ttcatccatt
     1081 gacggcattg cgcccttatt tttaatctcg gccataactt ttctccttga atgtttgata
     1141 acaataatgg tggttatatc tattaagtca tttttacctt atacttttct tgcgcgcagt
     1201 ctattgtacc ttggtacaat aaacctgttt tttttggggt ttgagtcctg atgttttctt
     1261 cgtttcctgc gggacggatc ggataaaaat caattgcctt gacatgagat atgatctgtg
     1321 attataaaaa cttacaagag tccatcatta ttcctttttc tgagaactga agtcaacaag
     1381 ctgctctcgt tcgttatttt ttaaaaaggg tcacgaatct tggcgcctcc taagtccatt
     1441 tataattcgc acaaaatcaa attagtatcg tttgcgatct tcattctcgt gggactcatc
     1501 gttaccggtt ctgttttcac ccgggaaaaa ttccatttga acgaaaaagt gcttcaaaaa
     1561 gcatatgaaa aatacggaca ggacgcaaag atgcgtctta tccactggga aaacagccta
     1621 aaaaaagaca gcataccgga gcgggaaaaa cttgaaacca taaactcttt ctttaatcag
     1681 caaatggttt tcgttaacga tacggatctt tatggcgtga aggattactg ggcaacccct
     1741 gttgaattca tctccagggg ggccggggat tgcgaggatt ttgccattgc caaatacttc
     1801 agcctcaaaa ttatgggcgt ggatgaagca aaactcagaa ttgcttatgt taaagccctg
     1861 cgacaaaaca ttttccatat ggtgatgttg tattacagtg atcccgcagc cgaaccgctg
     1921 gttctcgaca atcttgttga ttccatcaga tcagcctcgg agaggcggga tcttttaccg
     1981 atatttacgt ttaatggtgc cgggctctgg ctggctcacg accggcgaca gggaaaactg
     2041 gccggcaaaa gcagccgtct gacggcttgg aatgatcttc tgcaacgcat ggcaggaacc
     2101 ggaatttaaa ttcacaggag gggctccatg actctttaca ggcaacttgt tatatttacc
     2161 gtttcgttgt ttttcattct ttttgcagga acctggttcg ccaagtttga gagcacacga
     2221 ttgttcctga ccaatcagct tgaatcccat gctcaggata cggcaagttc tctggggctg
     2281 gccatttctc aatacccgga cgacctggtc agcgtggaaa ccatggtgaa cgccattttc
     2341 gacagagggt attacgagac catccgcttt atggatcccc acggaaaaat cttactggaa
     2401 cggaatctga aagtggtgat tgaaggcgtt cctgcctggt ttattaacct ggtcccgtta
     2461 aaagttccgg aagccaatgc ctacgtcacg gcaggctggc gtcaggccgg aaccatctat
     2521 gtaaaaagcc atgcgggcta tgcctacaag agcctctggc aagatatcaa acatacaacc
     2581 ctgctttttc tcttttgtgg aatgttcatt ttaatcgcag gcgggtttgg attgcgcatg
     2641 ctgctgcgtc cgctggtgct tgtcgaacag caggcagaag ccttatgcag aaaggaatat
     2701 gaaatccaga ccaaaatacc aagaacgaga gaactgcggc gcgtggtgca tgtaatgaac
     2761 cgaatgacgg aaaaagttaa agaaatgttt tccgaacagg tgacgcaggc ggaggggctc
     2821 agggagcggg cctataacga ttcattgacc ggtatcggca atcgccgcta tttcgaaagc
     2881 caggtcgccg cttatcttga caacaaagaa aacgacttca agggaatctt aatgcttata
     2941 cgtattcatg ggttggaaat actcaatgag cagagaggtt tccaggttgc cgacgaatta
     3001 ttaaagaaag ttgccgcgtc actgcaggaa atgtctgtaa atcttgccgg ttcggttctg
     3061 tccagacttt ccggaagcga ctttgtccta ttcctgcccg attatccctc atcagagtca
     3121 gatcggattt ccggtgaagt cgctaaaaaa ttgagcgggt tggaagccac ggggataacg
     3181 gcttcagaaa atgtttccaa tgtgggcacg tgtgcttttg accgttccgt gactctaggc
     3241 caacttcttg cagaagctga tttggcttta gctgcggcca atcaggccgg agcaaataca
     3301 tggcatgttc gtacaatcat ggagagtacc gccaagattc ccttcggcca gcagcagtgg
     3361 aaagacgctc ttgaaaaagt tctccagaac cgtcgcattg tcctgtattc acagcccgtg
     3421 gtcaaagccg gagacaaaaa cagtatcctt cacctggaaa ttttttcccg tattgttctt
     3481 gacacaggtg aaatcatcaa tgccgggata tttattccga ttgccgaacg tttgaagctt
     3541 atctcttctt tggatcgttt gattatagaa gaagtttcga aacttgatcg caagaagctc
     3601 ggcgcggaca ctatcgccgt caatttgtcg ccttcttctc tgcaggacga atcttttatt
     3661 cagtggataa gagatacttt actgcattta ccggaaaacg cgcccaggat catttttgaa
     3721 tttgcggaat tcggagccgt acagaatgaa aatctggtca ggtcatttat agcagtcatt
     3781 cgtgaatgcg gtcatgaaat gggattggat cattatggtc agagttgcgg aaatttaaga
     3841 caccttcagt cactgcgtcc cgattatgta aaaatcgacc gggcatacac cggagaactg
     3901 aaagatgaag acagcgacag ccgtttcttc atcagttcca tttgcagcgt cgcccatagc
     3961 atagatattg ctgttattgc cgaaggcgtc gaaacagata atcaatggca gctgttaaaa
     4021 gaactgaatg ttgatgctgt tcagggatat attatcaata gttcgaagcc gattacaaaa
     4081 gtttaagttt ctttgtgtcg ggcatgaaac ttaaagctgg ctttggagat attcgcagca
     4141 tatagaattt tgtattccgt ttatacgggt tgttctgccc tattcttaac ttgacggata
     4201 gtgttcaata aagatgtgcc tta
//
LOCUS       DAOSME010000124         4199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_22928_length_4199_cov_18.789817, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000124 DAOSME010000000
VERSION     DAOSME010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22928_length_4199_cov_18.789817"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLG98_11085"
     CDS             <1..188
                     /locus_tag="PLG98_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33851.1"
                     /translation="QNREYGLLKMSRKVGVSTYRITVKFDDDNFTIKGDIDTDIFNPF
                     IGEDAKIIEEAIKNAVK"
     gene            280..996
                     /locus_tag="PLG98_11090"
     CDS             280..996
                     /locus_tag="PLG98_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33852.1"
                     /translation="MENQNHYYIIWAIRLLLIISILANIVFITLHLLSDDLVTKKEII
                     EELSQRIELLFWSVVTLTLTFLPDYIEYKNIHLPHILEIIIVIFIFAGIFLSVRFELY
                     YNVFWWDDVLHTLSGVIIGFLGFIIIYKINSKYSMDISPLLVAVFAFTFAMTMGVIWE
                     IFEFAMDVFFGTTMQSWDLPHDTTLIGRAFQGSGLRDTMSDLIIDSIGALSTSIICYF
                     LYKEEKKKTLALMHEIFPDA"
     gene            1231..1845
                     /locus_tag="PLG98_11095"
     CDS             1231..1845
                     /locus_tag="PLG98_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE33853.1"
                     /translation="MIGNRQNIIDATDRLLQTHGLARLTTREIAREAGVAEGLIYHHF
                     KDKAELIFEVIETRVRETKNYMQNLPLEVGKNTLSKNLEDVLLSVYHAHYEITPIINT
                     VFADQKLRVRLQEIVKERNMGPRYAIEGLEVYLSAEQRLGRLSDAIDTGVLAKCLWMI
                     SIQSAMLDRWTENEVDEASVSAEIRKYIQTLMTGFAPRPKPKKR"
     gene            1892..3073
                     /locus_tag="PLG98_11100"
     CDS             1892..3073
                     /locus_tag="PLG98_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE33854.1"
                     /translation="MKRNKQVVPLIAVIILLSCLIVDGCGKKEETMAPPSPEVAVVTV
                     QSRQVVTTTELTGRTASNLIAEVRPQVGGIIQKRLFTEGADVRAGQPLFQIDPAIYQA
                     AVSNAGAALARSEAQLTTVQLRADRLRELLAEKAVSQQDYDDAAAALKQTQADIQYGK
                     ANLETARINLQYTTITAPISGRIGRSGITEGALVTAHQPLALATIQKIDPMYVDVTQS
                     TTDILKLRRKLQEGQLSRNSNQQQVRLILDDGSEYPFKGKLQFQDITVDPTTGSVILR
                     IVFPNPKGLLMPGMFVRAIVEEGVHKNAILVPQQAVSRDPKGNPLTLTVDKEGKVQQK
                     PLTLDRAIGSEWLVSSGLEPGDKVIVEGMLKVKPGIPVKAVPFDTGGKTEQANPPAKT
                     K"
     gene            3086..>4199
                     /locus_tag="PLG98_11105"
     CDS             3086..>4199
                     /locus_tag="PLG98_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE33855.1"
                     /translation="MLSKFFLDRPVFAWVIAIIIMAAGALAIYNLPISQYPPIAPPTI
                     AIDSFYAGASAETVENSVTQIIEQKMTGFDNLLYMSATSDSAGSSRIELTFKAGTDPD
                     LAWSQVQNKLQLAMSSLPETVRNQGVTVSKSTANYLMVVGLISEDGSMDRSDLEDYAK
                     SNLEKILARVPGVGEVEIFGSGYAMRIWLNPDKLTNYHLTIEEVLAALKSYNVEVSAG
                     QLGGAPALEGQRMNASIVVQNMLRTPEEFGAIPIRTNPDGSIIRMRDIGRTELGTEIY
                     DIQVEHNGKPSTGLAIRQAAGANALDTADRVKAKLAELSKFFPGGLKVVYPYDTTPFI
                     RVAINEVVKTLFEAILLVFLVMLLFLGNIRATLIPTI"
BASE COUNT         1144 a         1019 c         1027 g         1009 t
ORIGIN      
        1 atcaaaacag ggaatatggt ttgttgaaaa tgagccgtaa agtcggcgtc agcacttaca
       61 ggataacggt aaagtttgat gacgacaatt ttacaatcaa gggagatata gacaccgata
      121 tctttaatcc ctttatcggg gaagatgcca aaatcatcga agaagccatc aaaaatgccg
      181 taaaataatc gtctgcggcc caaacagacc atttttggtt ttcacatccg cgtgggatcg
      241 gcaaatattc aacaggacat gaatcagaaa aacagacaaa tggaaaatca gaatcattat
      301 tatattatct gggcaatcag gttattgctg atcatttcga tattagcaaa tattgttttc
      361 atcaccctgc atctcttgag cgatgacctc gtcaccaaaa aagaaatcat agaagaatta
      421 agccaaagga ttgaactgct tttctggtcg gttgtgacac tgacattgac attcttgccc
      481 gattatatcg aatacaaaaa tattcatctt ccccatattc tggaaatcat tatcgtgatc
      541 tttatctttg ccggaatttt cttaagtgtc cgttttgagc tgtattacaa tgtcttctgg
      601 tgggacgatg ttctgcatac cctttccgga gtcattatcg ggttcttagg attcatcatc
      661 atatacaaaa taaacagtaa atacagcatg gatatcagcc cgcttctggt ggccgtgttt
      721 gcttttacgt ttgccatgac catgggggtc atctgggaaa tttttgaatt tgccatggac
      781 gtatttttcg gaaccaccat gcagtcttgg gatttacccc acgatacaac gcttatcggc
      841 cgggcgtttc agggcagcgg cctgcgcgac accatgtccg atcttatcat tgacagtatc
      901 ggcgctttgt caacatccat catttgctac tttctctata aagaggaaaa gaaaaaaacg
      961 ctggcgctca tgcatgaaat atttcctgac gcttagagca acaaaataaa ccgcggtctg
     1021 aatcttgaac gaaacgtctg tgccataagc tattttttct gaagtttaag gcataaaata
     1081 ctcgcggctg agaaaacgac ggacctttgc aaaaaatcct tgacaatcat tgggtgtttg
     1141 tttagtactc ctgagtaaac actcagtgac ggacattgtt ttcatgatat ttccatgctt
     1201 atttgcgttt gaaatttcgg aaacccccaa atgatcggca atcgacaaaa tatcattgac
     1261 gccacagatc ggcttctgca gacccatggt ctcgcgcgac tgaccacacg tgaaatagcg
     1321 cgggaagcag gtgtcgccga aggtctgatt tatcaccatt tcaaagacaa ggcagagttg
     1381 atctttgaag ttatcgaaac ccgcgtacgt gaaacgaaaa actatatgca gaatctgccg
     1441 ctggaggtgg gcaaaaacac actctccaag aatttagaag atgttttgct ttcagtttat
     1501 catgcccact atgaaataac accgattatc aacacggttt tcgctgatca gaagttacgc
     1561 gtccgtctgc aggaaatcgt caaagaacgg aatatgggac cgcgatacgc cattgaagga
     1621 ctggaagtat atctgtctgc ggaacagaga ctggggagac tctcggatgc catcgatacc
     1681 ggcgtgctcg ccaagtgcct ttggatgatc agcattcagt ccgccatgct tgaccggtgg
     1741 actgaaaatg aagtcgatga agccagtgtc agcgctgaaa tccgcaagta cattcaaacc
     1801 ctgatgaccg gtttcgcgcc gcgcccgaag ccgaagaaaa gataaaatca aaaatatgca
     1861 atattgtttt atattacgaa agaggtgcct gatgaaaaga aataagcaag ttgtgccgtt
     1921 gattgcggtg atcattcttc tgagttgtct gatcgtagac ggttgtggca aaaaagagga
     1981 aaccatggct ccgccaagcc cggaagttgc cgtggtgaca gttcagtcca gacaggtcgt
     2041 gacaacaacc gaactaaccg gccggacggc ctctaattta atcgccgaag tccgcccgca
     2101 ggttggcggc atcatccaga aacgcctgtt tacggaaggc gccgatgtcc gggcgggaca
     2161 gccattgttt cagatagatc cggcaattta tcaggccgcg gtaagcaatg ccggggcggc
     2221 actggcccgt tccgaagctc aactcacgac cgttcaatta agagccgatc gcctgcggga
     2281 attgctggct gaaaaagccg tcagccagca ggattatgat gatgccgccg ccgcgctgaa
     2341 acaaacgcag gcggatattc aatacggcaa agcaaatctc gaaaccgccc gaattaatct
     2401 tcaatatacg acgataaccg cacccatttc cggtcgcatc ggtcgatccg gcataacgga
     2461 aggcgccctg gtcacggcgc atcagccttt ggcgctggcc accattcaga aaatcgatcc
     2521 gatgtatgtg gatgtcacgc agtccacgac tgacattctg aaattgcgcc gtaaacttca
     2581 ggaaggacaa ctgtccagaa acagcaatca acaacaggtg cgactcattc tggatgacgg
     2641 ttcggaatat ccatttaagg gcaaactgca atttcaggac atcaccgttg atccgacgac
     2701 cggatcggtc atcctgcgta ttgtatttcc taatccgaaa ggtttattga tgccgggcat
     2761 gtttgtccgg gccattgttg aagaaggcgt ccataaaaat gccattctgg ttccccagca
     2821 ggcggtttcc cgcgatccca aggggaatcc tctgacgctg acggttgata aggagggaaa
     2881 agttcaacag aagccgctaa ccctggaccg cgccatcggc agtgaatggc ttgtttcatc
     2941 gggactggaa cctggcgata aggtgatcgt ggaaggcatg ttgaaagtaa aaccgggaat
     3001 tcccgtaaaa gccgttcctt ttgataccgg cgggaaaacc gagcaagcga acccgcctgc
     3061 aaaaacgaaa taacggagga gcctgatgct atcaaaattt tttctggatc gtccggtttt
     3121 tgcctgggtc atcgccatca tcattatggc ggcgggagcg ctggccattt acaatctccc
     3181 catttcccag tatcccccaa tcgctccgcc gacgattgcc atcgattcct tctacgccgg
     3241 cgcatccgct gaaaccgtcg aaaacagcgt cacccagatc attgaacaga aaatgaccgg
     3301 ctttgataat ctgctgtata tgtcggccac cagtgattcg gcaggatcgt cgcgcatcga
     3361 actgactttc aaagcgggga cggatcccga cctcgcctgg tctcaggttc agaacaagct
     3421 gcagctggcc atgtcgagcc taccggaaac agtcagaaat cagggagtca ccgtcagtaa
     3481 gtcgacggcc aattacctga tggttgtagg cctgatttcg gaagacggca gtatggatag
     3541 aagcgacctg gaggactacg caaaatccaa tctcgagaaa atcctggcgc gggtcccggg
     3601 tgtgggtgaa gtggaaattt tcggcagcgg ttacgccatg cgcatctggc ttaatcccga
     3661 caaacttacc aattatcatt taacgattga ggaagttctg gcggcgttga aatcctacaa
     3721 cgtggaagtt tccgccggtc agctgggtgg cgctccggcg ctggaaggac agcggatgaa
     3781 tgcctccatt gtcgtccaga acatgttaag aacccccgaa gaattcggcg ccatccccat
     3841 ccgcaccaac ccggatgggt ctatcattcg gatgcgcgat atcggacgga cggaattagg
     3901 gacggaaatt tacgacatcc aggttgaaca taacggcaag ccttcaaccg gtctggcaat
     3961 ccgtcaggcc gcgggcgcga acgccctcga tacggcggac agggtcaagg caaaacttgc
     4021 cgagttgagc aaattcttcc ccggaggact gaaggttgtc tatccttatg acaccacgcc
     4081 tttcataaga gtagcgatca atgaagtggt taaaacgctc ttcgaagcca ttctgctggt
     4141 ctttctggtc atgctgcttt ttctgggcaa tatccgggcc acgctgattc cgaccattg
//
LOCUS       DAOSME010000125         4174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_23097_length_4174_cov_23.865259, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000125 DAOSME010000000
VERSION     DAOSME010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23097_length_4174_cov_23.865259"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..699
                     /locus_tag="PLG98_11110"
     CDS             235..699
                     /locus_tag="PLG98_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOE33856.1"
                     /translation="MELKSLSFQHENMIPAQYTCDGRNVSPPLSWSGAPDKTKSFALI
                     CDDPDAPVGLWVHWVLFDIPASVNALAENFSRTEDIPGFGKNGKNTSGRFGYDGPCPP
                     DGTHRYYFKLYALDTMLNLKPGLTKDDLLQAIKGHVLAEAQLMGRYKRPAGR"
     gene            complement(734..1621)
                     /locus_tag="PLG98_11115"
     CDS             complement(734..1621)
                     /locus_tag="PLG98_11115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33857.1"
                     /translation="MMQHIEYLKMLAESGGPRESQYEELNRFYQLFGQQRKQKIISEN
                     EVVSIWHELSDVFCTQDTMQGYAVLKPHGYAGDFIIIERIYQHWLSPKEHLVNWDRFF
                     HAQAAPRAILNRKQYFIDILNALKKKDGRCDVLNIGCGPCQDVVEFLAQGKSHVFFEC
                     VDYDKNAIDYSYNKIKENGFLSNLEFHRDNAFKFRPSRQYDLIWSAGLFDYLDEKTFK
                     MLLTKQLAFLKEGGEMVIGNFSLNNPTRDYMEFGNWFLHHRSPEELIQIACDCGIPED
                     TIDVKSEPLGVNLFLHISK"
     gene            complement(1689..3338)
                     /locus_tag="PLG98_11120"
     CDS             complement(1689..3338)
                     /locus_tag="PLG98_11120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE33858.1"
                     /translation="MNMKLHPITLRFSDENAHLEALFQKDYYRISLSTMRLFVFLGAV
                     LYASFGILDAYVMPEQKIIIWFFRFIIVGPVLLGVLLISFTRLFKKYMQPILAALYVL
                     AGTGIVAMIVVAPPPVSYSYYAGMMLVFMWGYTLIRLLFIWASLAGWFQVALYEITAV
                     FINPTPLDILISNNFFFISANLVGMMACYAIEFYARRDFFMKKQLESERENVSRINAE
                     LEERVAKRTEDYRVVNRALQEEVAGHREAEKALRDSEERYRALVENASDMVFRTDIHG
                     RFTFANVSTIRVSGYDESEIIGKHYLELLRPDMREDAAGFFGLQIKNRIRNTYSEYPV
                     IKKDGSEIWLGQNTQLITEDGKVIGFQAVSRDVTERRRLEKELKESEERYRELSIIDD
                     LTQLYNSRHFYSQLQMELGRIERREYPLTLLLLDIDDFKVFNDTYGHIEGDRVLVRLG
                     QVIKKCLRKEDSAYRYGGEEFTIILPMTTKEEGAVTAERVREELRKENFSSVPDKEIY
                     LTVSIGLAQYEKNESIKSFVNRVDHFMYRSKKNGKDRVYFE"
     gene            complement(3534..>4174)
                     /locus_tag="PLG98_11125"
     CDS             complement(3534..>4174)
                     /locus_tag="PLG98_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33859.1"
                     /translation="LSVPSLRAAYSSSGMLDRSDSLYFYRKTGLGITEVYGSTETGGV
                     ALRSVSEQTESWKPFDVVRWKLAGSRLSVRSDFTSPEMEYDAEGFCLTGDEAREDRDN
                     RFILLGRADGIVKIAGKRVDLADVQNKIKSLPTVNDAYVLALTTDKGRSSDIAALVAC
                     DLTETHLKKLLMEKLEPYAVPRRIKIVSSITRTATGKIDRRRVEKLFIPDNK"
BASE COUNT         1135 a         1038 c          891 g         1110 t
ORIGIN      
        1 gattaaggtt catgatacca caggatcatc aggtgtcaac agcgttcgtt ggcgctattg
       61 acgattttaa aatctccggt taaaataccg gcataagctt ggcagatcga aaatggtgaa
      121 acaatgaaaa tttatttctt ttgtttttct gtgattattt gtttcattat tggcaacgcc
      181 gtcaccgttc aagcccatca acgaggcagt ttcaaaacta aaggaggaaa gaccatggaa
      241 ttaaaaagtc tgtcttttca gcatgaaaat atgattccgg ctcaatatac ctgtgacggc
      301 cggaatgtat ctccgccact ttcgtggagc ggcgctcccg acaagacaaa aagtttcgcg
      361 ctgatctgcg atgatcccga cgcaccggtc ggtttgtggg tgcactgggt ccttttcgac
      421 atccccgcat cggtcaatgc ccttgcggaa aatttttccc gaacggaaga cattcccgga
      481 ttcgggaaaa acgggaaaaa cacgtccgga cgtttcgggt atgacggccc ctgccctccg
      541 gacggcacac accgctatta ttttaaatta tatgcgctcg atacgatgct gaatctcaag
      601 ccgggcctga ccaaagacga cctgctccag gcgatcaaag gacatgtcct ggcggaagca
      661 cagttgatgg gccggtataa aagaccggca ggcagataac gtcgttcgac ggaaaagaaa
      721 tccgcaaggc ttcttacttg gaaatgtgca ggaaaagatt gacgcccagc ggttccgatt
      781 ttacatcgat ggtgtcttcg ggaatgccgc aatcgcaggc tatttgaatc aattcttccg
      841 gcgaacggtg atgcaggaac cagttgccga actccatata atcgcgggtg ggattattga
      901 gcgaaaaatt gccgatcacc atctccccgc cttccttgag aaaggcaagt tgtttggtca
      961 gcagcatttt gaaagttttt tcatccagat aatcaaaaag gccggccgac cagatcaaat
     1021 catattgccg cgacggcctg aatttgaaag cgttgtcgcg atggaattcc aaattgctca
     1081 aaaagccgtt ttctttaatt ttattatagg aataatcgat ggcgtttttg tcgtaatcca
     1141 cgcattcaaa aaacacatgg gatttcccct gggctagaaa ttccaccaca tcctgacagg
     1201 gaccgcagcc gatatttaaa acatcgcatc ggccgtcttt tttcttgagc gcattcagga
     1261 tatcaataaa atattgtttc cggttcaaaa tggcgcgcgg ggcagcctga gcatgaaaga
     1321 agcgatccca gttgaccagg tgctccttcg gcgacagcca gtgctgataa attctttcaa
     1381 tgataataaa gtcaccggcg taaccatgag gttttaaaac agcgtatccc tgcattgtat
     1441 cttgcgtgca aaacacatca gacaattcat gccagatact gaccacttcg ttctcactga
     1501 tgattttttg tttgcgctgc tgaccgaaca gttgataaaa gcgattcaac tcttcatact
     1561 gactctcccg ggggcctccg ctttcagcca acattttcag atattcaata tgttgcatca
     1621 ttcgttctcc ttaaccttca ttattgcatt cataccataa ttacagcatc agttctatcg
     1681 atttatcatt attcaaagta cactctgtcc ttgccattct ttttgctgcg atacataaaa
     1741 tggtcaaccc ggttgacgaa cgattttatg ctttcatttt tctcatactg cgccaggccg
     1801 atactgaccg taagatagat ttctttatcc gggacagaag aaaaattttc ttttcttaac
     1861 tcttctctga cccgttccgc cgttacagcg ccttcttcct tcgtcgtcat cggcaaaatg
     1921 atggtaaatt cttcaccgcc gtaacgatag gcggaatctt ccttgcgcag gcacttttta
     1981 atcacctggc ccagccgcac taaaacacga tctccttcaa tatgtccgta cgtgtcgttg
     2041 aagactttaa agtcatcaat atcaaggagt aaaagggtca acggatattc acgtcgttca
     2101 atccggccca gctccatttg aagctgacta taaaaatgtc tggaattata aagctgcgtc
     2161 agatcgtcaa tgatactcag ctcgcgatac cgttcttcgc tctcttttag ttctttttcc
     2221 agacgtctgc gttcagtaac atcacgggat accgcctgaa aaccgatcac cttgccgtct
     2281 tccgtaatca actgtgtatt ttgtcccagc cagatttcgc tcccgtcctt cttgattacg
     2341 ggatattccg aataagtgtt tctgattctg tttttaatct gcagaccgaa aaatccggcg
     2401 gcatcttcac gcatgtccgg gcgtaaaagc tccagatagt gtttgccgat aatttcagat
     2461 tcatcatatc cggaaacgcg aatcgtcgat acattggcaa aggtaaaccg tccgtgaata
     2521 tcggtacgaa aaaccatatc actggcattt tccaccaggg ctcggtaacg ttcttcgcta
     2581 tcccgcaacg ccttttcagc ctccctgtgg ccggcgacct cctcctgaag agcccggttc
     2641 acaacccggt aatcctccgt gcgcttggca accctttctt ccagctccgc attgatcctg
     2701 ctgacatttt cccgttcgct ttccagctgt tttttcatga agaaatcgcg gcgcgcgtaa
     2761 aactcaatgg cataacatgc catcataccc accagattag cactgatgaa gaaaaaattg
     2821 ttgctgatca aaatatcaag aggtgtggga ttaatgaaaa cggcggtgat ttcatacaac
     2881 gctacctgaa accagccggc cagtgacgcc catataaaca gcagacgaat cagcgtgtac
     2941 ccccacatga aaacaagcat cataccggcg taataggaat aactgaccgg tggaggggcc
     3001 acaacgatca tcgcaacaat gccggttccc gccagaacat aaagcgcagc cagaataggc
     3061 tgcatgtatt ttttaaatag acgggtgaag gatatcagta aaacgcccag caacactgga
     3121 ccgacaataa taaaacgaaa aaaccatatg ataatttttt gctccggcat gacataggcg
     3181 tccagaattc cgaaagacgc ataaagtacc gcacccagaa agacaaagag ccgcatcgtg
     3241 gacagcgata tcctgtaata gtctttctga aacaatgctt cgagatgagc gttttcatct
     3301 gaaaaccgga gggttatcgg atgcagtttc atgttcataa attattctgc aatctgaata
     3361 tgaggtaacg tcttacatta attacggatg ccggacagcc ttctacattg ggaattattt
     3421 ttgatacgta tcataattac cggcaaataa caatacaagc acaaaaactt tcataatatt
     3481 tataaatgtt tgtaatattt tctgacgaga aaaccggctt tttgaaccgc cggttattta
     3541 ttgtcgggta tgaaaagttt ctctacacga cgacggtcta tcttaccggt cgctgttcgg
     3601 gtaattgaag acacaatttt gatacggcgg ggaacagcgt aaggttccag tttttccatc
     3661 aaaagctttt tgagatgcgt ttctgtcaga tcgcaggcaa ccaacgccgc aatatcgctg
     3721 gaccgcccct tatccgtggt cagcgccaga acatacgcat cattgaccgt gggcaacgat
     3781 ttgattttat tctggacatc ggccagatcg actcttttcc cggcaatctt gacaatacca
     3841 tcggctctgc ccagaagaat aaaacgattg tcccgatctt cgcgggcttc gtcgccggtc
     3901 aggcaaaatc cctccgcatc gtactccatt tcaggcgagg taaaatccga ccgtaccgat
     3961 aatctgctgc ccgccagttt ccaacggaca acatcgaagg gcttccagct ttccgtttgt
     4021 tcgctgacgg aacgcagagc aactccgccg gtttctgtgg aaccgtaaac ttccgtaatg
     4081 cccaggcctg tctttcggta aaaatacaaa ctgtcagaac gatccagcat tccggaagaa
     4141 gaatatgccg ctctcagtga aggaaccgac agat
//
LOCUS       DAOSME010000126         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_23503_length_4122_cov_28.263339, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000126 DAOSME010000000
VERSION     DAOSME010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23503_length_4122_cov_28.263339"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1397)
                     /locus_tag="PLG98_11130"
     CDS             complement(93..1397)
                     /locus_tag="PLG98_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOE33860.1"
                     /translation="MLDLLSLPGICPVDLQSKAGVIIISAESPDVDLPDCPICGKPMY
                     RHGRRMNMFADTPMQMQPVRLEITRPRYRCFTCGKMLMSELPFLDENRRATKRLVDSI
                     RERCLGMTFISLARQTGLAVNTIKNIANDLIKELDRTIHYETPVIMGIDEVNLAGGYR
                     CVITNLATNNVFQMLEHRTQAHLKPFFASLPDRDKVEWVCTDMWRPFKESFGLYLPNA
                     RLVIDKFHVIKMASEALDMERKIYQEDLSRNERIHIKKSLRWITLKRPDKLTSAEKSA
                     MEVIREKYPQLAEAYDIKEEFYRIYDSPDKESAINAFKAWKDSLPSKGLNGFHKLVRT
                     VENHCEDIFAYWDSPNQITNAYTECLNGLVKVANRMGRGYSYEIIRAKTLYEKHARKV
                     GSGVRSKSELQAPGSTIDTTQSVEYGPHIPTLVNLVETGQVS"
     gene            complement(1390..>4122)
                     /locus_tag="PLG98_11135"
     CDS             complement(1390..>4122)
                     /locus_tag="PLG98_11135"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE33861.1"
                     /translation="MEQYRTDRLPYDPKTKRREPLLTFKRGAIVHFAMSDSEIQMATK
                     LDGENTVFLPFNQGNEGRAGNPSRTDGEYPVAYFWEAVCQRDAWLRIFHSFIYVEKKD
                     VVDLKGNWSKKETLIFPRFHQWAAVNAMIEDAKTHGSGMQYLCEHSAGSGKTSTIAWI
                     AHDLIKLRRDNGDAIFNSVIIVTDRKVLDSQLQDAVKQIDHQFGVIAAIDRKKSSKSK
                     SKLLAEALFAGTPIIVVTIQTFPYAMEAIVTEKKLRNKSFAVIIDEAHASQTGSTATK
                     LQAALSLSAKGGMSNLTVEELLEKLQKSRVRPNNISHFAFTATPKHSTFMLFGRPDDL
                     LKPASKDNIPHAFHRYPMRQAIDEGFILDVLRGYVPYKTAFNLAKQIEDTKRVSGKQA
                     KRALAQWMALHPTNVTQKVQFIIEHFSKNIEHLLDGKAKAMVVTSSRAAAVRYKKGFD
                     RYIEKHPEHAGIRSLVAFSGKLTGKQVIHADDERLTGDVFIVDEKEEFTEISMNPEIA
                     GQDLRLAFERPEYRVMLVADKFQTGFDQPRLVGMYIDKKIANDVEIVQTLSRLNRTAP
                     GKDEIFIIDFVNDPANIRRAFSIYDEGAVIDDVQDLNVVYEIKESLDAQGIYETEDLE
                     NFKQARFKTIRDITQAQEPQHKALFTATDRPTRLFNQRLKMMRDAAATWESAYEKARH
                     SPGDKAGMQSADHHRRDYAEQIANLMTFKSGLGRFCRTYSYVSQLIDFGDPELENFAA
                     FAKLLQKRINGEPLETVDLKGLVLTGFDIKAKMDMIPEETDKPSLKPVGPGGCVGCDD
                     PNYLREIIERLNRLFGDATPIRDQAIFVNHIVAIAKENDVVMAQVENNAREQALKGNL
                     PGAVQQGVVRALSSHQKLATLVLKSDRQAMSALTDVIYDLIHDHSTIDVDKIDA"
BASE COUNT          990 a         1013 c          961 g         1158 t
ORIGIN      
        1 acatatctta tcgcctaagc aagccggatg ccggcaagag aatggtggaa tctatggcta
       61 tgtgcaatac agggttgaaa tcatcctaat aactacgaaa cttgaccagt ctcaaccaaa
      121 ttgaccaagg ttggaatatg cggtccatat tcgacggatt gagtagtatc aatggttgaa
      181 cccggggctt gaagctcgct tttggagcga actccactgc caacttttcg ggcatgtttc
      241 tcatacaacg tcttagctct tatgatttca tagctatacc cccgccccat ccggttggct
      301 actttcacca gaccattcaa gcattctgta tatgcgttag ttatttgatt gggagaatcc
      361 caatacgcga agatgtcttc gcaatgattt tcaactgtcc taaccagttt atggaatcca
      421 ttgagcccct tagaaggaag ggaatctttc catgccttga atgcattgat cgctgattct
      481 ttatccgggc tatcgtagat ccggtaaaac tcttctttaa tgtcataagc ttcagctaat
      541 tgtggatatt tctcacgaat cacttccatt gcagattttt cggctgacgt taatttatct
      601 ggccttttca aagtgatcca gcggagtgac ttcttaatgt ggatacgttc gttccgagac
      661 aaatcctcct gataaatttt acgctccata tccagtgctt ccgatgccat tttgatgaca
      721 tgaaacttgt cgataaccaa acgagcgttc ggcaggtata gaccaaatga ttccttgaac
      781 ggtctccaca tatccgtgca aacccattca actttatcac gatcaggcaa tgaagcaaaa
      841 aagggtttca ggtgggcctg ggtgcgatgc tctaacatct gaaagacgtt gttggtcgcg
      901 agatttgtaa taacacaacg atagccgccc gcaaggttca cttcgtcaat tcccatgatg
      961 acaggcgttt cgtaatgaat cgtccgatcc agttccttga tcaagtcgtt cgcaatgttt
     1021 ttgatagtgt ttactgccag acctgtttgg cgcgcaagac ttatgaatgt catcccaagg
     1081 caacgttctc ggatggaatc gacaagccgt tttgttgctc tgcggttttc atccaggaat
     1141 ggcaattctg acattaacat cttgccgcaa gtaaagcagc gatagcgagg tcgcgtaatt
     1201 tccaaacgta ccggttgcat ctgcatggga gtgtcggcaa acatattcat cctgcgtcca
     1261 tgccgataca taggtttccc acaaataggg caatccggta aatcgacatc tggcgactcg
     1321 gcagaaatga taataacccc agctttagac tgaaggtcca ctgggcatat tccaggcaaa
     1381 cttaataaat caagcatcta ttttgtccac atcaattgtg ctgtgatcat gaattaggtc
     1441 ataaattaca tcagtaagag cagacatagc ctgcctatcg gatttaagaa ccaaggtggc
     1501 tagtttctga tgagagctga gtgcccttac gacaccctgc tgtactgcac cgggcaggtt
     1561 gcctttcaac gcctgctcgc gtgcattgtt ttccacctga gccatgacca catcattttc
     1621 tttagctatt gctacgatat gattgacgaa tatggcttgg tcccgtattg gcgtagcatc
     1681 accaaaaagt cggttcagac gctcgatgat ctcgcgaagg taattgggat catcacatcc
     1741 cacacaccca ccaggtccca ccggcttcag tgaaggcttg tctgtttctt caggtatcat
     1801 gtccattttg gccttgatgt caaaaccggt cagcaccaga ccttttaaat ctactgtttc
     1861 gagcggttcc ccgttaatcc gcttttggag aagcttggca aacgctgcga agttctccaa
     1921 ctccggatct ccaaaatcaa tcagttggga aacgtaggaa taagtgcggc agaaacggcc
     1981 cagcccgctc ttgaaggtca taaggttggc aatctgctcg gcataatctc tacgatgatg
     2041 gtcagcagat tgcattcctg ccttatcccc tggactatga cgagcttttt cgtaagccga
     2101 ttcccaggta gctgcggcgt cacgcatcat cttcagccgc tgattaaaga gacgggtcgg
     2161 ccggtctgta gctgtaaaaa gagctttatg ttgtggttcc tgcgcctgcg ttatgtctcg
     2221 gatcgtcttg aaccgtgcct gtttgaaatt ctcaaggtct tctgtctcgt atatcccttg
     2281 agcatccagt gattccttga tctcgtaaac cacgttgaga tcctgcacat cgtctatcac
     2341 cgctccttcg tcgtaaatag agaacgcgcg ccgtatattg gcagggtcgt tcacgaagtc
     2401 gataatgaat atctcgtcct tacccggcgc cgtccggtta agtcgggaga gtgtctgtac
     2461 gatttccacg tcgttggcaa ttttcttgtc gatatacata cccaccagcc tgggttgatc
     2521 aaagccggtc tggaacttgt ctgctaccag catgaccctg tattccggac gctcgaaggc
     2581 caagcgaaga tcctgccctg caatctccgg gttcatactg atttcggtga actcttcttt
     2641 ctcatccacg atgaatacat caccagtcag gcgttcgtcg tcagcgtgta tcacttgttt
     2701 tccggtaagc ttaccggaaa aggcaaccaa agagcggatg cccgcgtgtt cgggatgctt
     2761 ttcaatgtag cggtcgaacc cctttttgta tcgcacggct gcggcacggg agcttgtaac
     2821 aaccattgct ttggctttgc catccagcaa gtgttctata tttttactga aatgttcgat
     2881 tataaactgt actttttgag tgacattggt tggatggagt gccatccact gtgccagggc
     2941 tctctttgcc tgcttgccgc tcaccctctt ggtgtcctca atctgtttgg cgagattgaa
     3001 agcggttttg taaggcacgt aaccgcgcag aacgtcgagg atgaaaccct catcaattgc
     3061 ctgccgcatc gggtaacggt gaaaggcatg cggtatgttg tcctttgagg ctggctttaa
     3121 tagatcgtct ggcctgccaa acaacataaa ggtagaatgt ttgggcgttg ctgtgaaagc
     3181 gaaatggctg atattattgg gccgaacccg cgatttttga agtttttcca gtaattcctc
     3241 caccgtcaga tttgacatgc cacccttcgc gctcaacgaa agggcagcct gaagtttagt
     3301 ggctgttgat ccggtctgag atgcgtgagc ttcgtcgata atgacagcaa agcttttatt
     3361 cctcagtttc ttttcagtga cgatggcctc cattgcatac ggaaaggtct gaatcgttac
     3421 gacgatgata ggtgttccag caaacagagc ttccgccagt aatttacttt tggacttaga
     3481 tgatttcttt cggtcaattg cagcaattac tccgaactgg tgatcaatct gctttaccgc
     3541 gtcctgaagc tggctatcaa gcaccttgcg gtcggtaaca atgatcacgc tattaaatat
     3601 ggcgtcgccg ttatcgcggc gaagcttgat gagatcgtga gcaatccatg caatggtgct
     3661 ggtcttacca gaaccagcac tgtgttcgca gaggtactgc atgcccgatc catgggtctt
     3721 ggcatcctcg atcattgcat tgaccgctgc ccactgatgg aaacgtggaa aaatcaaagt
     3781 ttccttcttt gaccaattgc ctttcagatc caccacgtcc tttttttcga catagatgaa
     3841 gctgtgaaag atgcgtaacc aggcatcgcg ctggcacact gcttcccaga agtaagccac
     3901 cgggtattca ccgtctgttc tcgacgggtt gccggcacgg ccttcattac cttgattgaa
     3961 gggcagaaag acggtatttt cgccatccaa cttggtcgcc atttggatct cggaatccga
     4021 catcgcaaag tggacaatag cgcctcgctt aaaagtcagc agaggttcac gacgcttggt
     4081 cttgggatcg taaggaagac ggtcagtgcg atactgttcc at
//
LOCUS       DAOSME010000127         4100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_23652_length_4100_cov_28.992089, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000127 DAOSME010000000
VERSION     DAOSME010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23652_length_4100_cov_28.992089"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="PLG98_11140"
     CDS             complement(<1..1556)
                     /locus_tag="PLG98_11140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE33862.1"
                     /translation="MENFELRQYQKEALDKLDQELKVKQNVLFVAATGAGKTVTICRM
                     INTYYKETDRRFLILVNKQELIMQFHADLMRKTSIPERELTICCAGLKSKYVDGRVTI
                     ATVQSFIGMMDSYPGADLVILDEVHGVTVDGEYGKVLNYLKSKKPHMRILGCTATPYR
                     LGHGMIYGSACVAPEKNLFDSISHRITYQQLRDAGYLVSLRGKVAHADSLTRDLSMVT
                     VSGDYVLNQLGEIMCREVHLSTGREAVKEYCKEFKRVCVFCCTIDHAEKLKKLINEDE
                     PCVTVHSQLSQLERIIALNTWKSGNARICTSVNILAEGFDYPALDCLVFARPTLSARL
                     FVQAIGRVLRTSPGKESGFLLDLTDNTARFGTDIDKIKADIPKRVIEGKEKKDAIWKF
                     CPQCAAECHQSLRKCESCGYEWPAPEIVEAAFVPEMKDVSFEPDPPVVVRPDEIYMTI
                     HQSKNGKQLGKCSFICNRFKFLSVWFCMEDYYNGYAVTAGAKRWKEMGGLDPYPTSCE
                     EFERRAVNEFV"
     gene            complement(1538..>1850)
                     /locus_tag="PLG98_11145"
     CDS             complement(1538..>1850)
                     /locus_tag="PLG98_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33863.1"
                     /translation="ATEAQIQRGVLDYLELLSRKKPIYYFRSAAGMVRTEQGRVFKTG
                     KPGAPDISVCFDGLFIGLEIKTTTGRQSALQKKAQAEIEAAGGKYYIIRCLEDIWKIL
                     S"
     assembly_gap    1851..1950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1951..2378
                     /locus_tag="PLG98_11150"
     CDS             <1951..2378
                     /locus_tag="PLG98_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33864.1"
                     /translation="ISVAKYRNEDANWTASGFLKGGNMKDLLKNFLKAKSIEDAAREG
                     RIAAEEAIIAKMGNLKLEGTTTKEVENYKVSVTTKLTRTLDYDKYVALGLPKALQFVD
                     LKPTINLTAYRAASLVNPAIALCVTSKPAKTSVKVEVIE"
     gene            2375..3094
                     /locus_tag="PLG98_11155"
     CDS             2375..3094
                     /locus_tag="PLG98_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33865.1"
                     /translation="MNLQDLIKTTRQSKPPRIVLHGIHGIGKSTWAAGAPAPIFIQTE
                     DGLVAIDVPHFPVAKTLDEFFGYMDMLINQSHEYKTVVIDTADWLEKLIWKTVCDENK
                     VTSIEKIGYGKGYVFAMQYWEKFFNGLNLLRDKGMACVILAHNEIKLFSPPDGDPYDR
                     FQIKLHKTAAARLEEWADVVLFAGFAVTVNADTGKAINNAERVIHTTNKPAWRAKTRY
                     VLPDTLPLNFVELLTAIKSNQ"
     gene            3110..3565
                     /locus_tag="PLG98_11160"
     CDS             3110..3565
                     /locus_tag="PLG98_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HOE33866.1"
                     /translation="MANLQGINIDANVPEAGSFIVVPEGVYKAVIAGDKIVSTKDGKG
                     KILELTIQIIDGSHTGSTIVDRLNIVNASQQAQNIAQGTLKRICGVLRAPFPPQNTDS
                     LMGKPMLVTVGVEEFTSNKTGNVLKSNKIKNYAPIPSVTSTTPAAVQGW"
     gene            3559..>4100
                     /locus_tag="PLG98_11165"
     CDS             3559..>4100
                     /locus_tag="PLG98_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33867.1"
                     /translation="MVNSIIDMIEAKRAGDNIPRQYLGLSEIGHKCPRWLWYAHHNTP
                     SKPIEGRIIRLFRTGNIIEDAIISDLESIGIEVTDRQREVEIVNGDIVLKGHIDGIVS
                     GQLLEIKSAGEKYFKQLLKVGYEKWNPKYRAQAHVYMVLCDLEECMVVVENKNDSNLY
                     IETLKLDRDYVTKLLIDVFAA"
BASE COUNT         1135 a          977 c          898 g          990 t
ORIGIN      
        1 acaaactcat tgaccgcccg gcgctcgaat tcttcgcacg atgtcggata cggatcaagc
       61 ccgcccattt ccttccagcg tttcgctcca gccgtcactg catagccatt gtaataatct
      121 tccatgcaaa accagactga taaaaacttg aaacgattac agataaatga gcatttgcct
      181 aactgcttcc cgtttttaga ttggtggatt gtcatataga tttcatcggg ccggacaact
      241 accggcggat caggttcaaa cgaaacgtcc ttcatctctg gcacaaacgc cgcttcgact
      301 atttccgggg ctggccactc gtagccgcat gactcgcatt tacgcaatga ctgatgacat
      361 tcggcagcgc attgcgggca gaatttccat attgcgtctt tcttttcctt gccttcaata
      421 acacgttttg ggatatccgc cttgatttta tcgatgtccg tcccaaatct ggccgtatta
      481 tccgtcaggt caagcaggaa gccgctttct ttgcccggac tggtacgcaa taccctgccg
      541 attgcctgaa cgaacaggcg ggcgctcagg gtgggcctgg cgaataccag acaatccagg
      601 gccggatagt caaatccctc cgcaaggata ttgacgctgg tgcaaattcg ggcatttccc
      661 gacttccatg tattcagggc aattattcgt tcaagttgtg ataattgact atgaacagtc
      721 acgcaaggct cgtcttcatt aattaatttt ttcagctttt cagcgtgatc gatggtgcag
      781 caaaaaacgc atactcgttt aaattcttta caatattctt ttactgcttc ccgcccagtc
      841 gataaatgaa cttctcgaca cataatttca cctaactggt ttaaaacata atcgccggac
      901 accgtaacca ttgataaatc tctggtcagg ctgtcggcat gagcaacctt gcctctcaat
      961 gataccagat acccggcatc acgtagctgc tgatatgtga ttcggtgcga aatactgtca
     1021 aacaggtttt tttccggagc gacgcaggcc gaaccgtaga tcattccatg acctaagcgg
     1081 tacggagtgg cagtgcatcc gagtattctc atgtgtggtt tttttgattt taaataattg
     1141 aggaccttac cgtattcacc atcaactgta acaccatgaa cctcatcaag aataacaaga
     1201 tccgcgccgg gataactatc catcatcccg ataaacgatt gaacagtggc aatagtaacg
     1261 cgcccatcga cgtattttga tttcaatcca gcgcaacaga tagtcaattc acgctctgga
     1321 atgctggttt ttctcatcag gtcggcatga aactgcatga tcaattcctg tttgtttacc
     1381 agaatcaaaa aacgtcggtc agtttccttg taatatgtgt tgatcatccg acagatcgta
     1441 actgttttgc ccgcccccgt tgccgcgaca aacaaaacat tctgcttgac ctttaattcc
     1501 tgatctaatt tgtccagggc ttccttctgg tattgtctta actcaaaatt ttccatatat
     1561 cctcaaggca acgtattatg taatattttc ccccggcggc ttctatttct gcttgcgctt
     1621 ttttctgcaa tgctgattgc cgtccggtgg ttgtttttat ctccagacca atgaacaaac
     1681 catcaaaaca cactgaaatg tcaggagctc caggctttcc tgttttaaaa acccttccct
     1741 gctctgttcg taccattcca gcggcggacc ggaaataata gataggtttt tttcttgaca
     1801 ggagttcaag atagtccaag actccccgtt gaatctgcgc ttctgttgct nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttataagcgt agccaaatac agaaacgaag
     1981 atgcaaactg gactgcgtcg ggatttctca aaggaggcaa catgaaagat ttattgaaaa
     2041 atttcctcaa agcaaaaagc attgaggacg cggcgcgtga aggacgcatc gcagcggaag
     2101 aggccatcat cgcaaagatg ggaaatctta aactcgaagg cacgacgaca aaggaggtcg
     2161 aaaactacaa agtctccgtc acaaccaaac tcacccggac gctcgactat gacaaatacg
     2221 tcgccctggg cttgccgaaa gcactccagt ttgttgatct taaacctaca atcaacctga
     2281 cggcatacag ggcagcttcg cttgttaatc ccgccattgc cctctgtgtg accagcaaac
     2341 cggcaaagac cagcgttaaa gtggaggtga tcgaatgaac ctccaagact tgatcaaaac
     2401 cacccgacaa agtaagccgc cccggatcgt tctgcatggg atccacggca tcggaaagtc
     2461 cacatgggca gcaggcgcac cggcgccgat attcatccag accgaagatg gcctggtggc
     2521 tatcgatgtt ccgcattttc cggtagcaaa aacccttgat gaatttttcg gctatatgga
     2581 catgctgatc aatcagtcac acgagtataa aacggtcgtt atcgacaccg ccgactggct
     2641 tgagaaactt atctggaaga cggtttgcga tgaaaacaag gtaacttcca tcgagaaaat
     2701 cggttatgga aaaggttatg ttttcgccat gcagtactgg gaaaagtttt tcaacggact
     2761 gaacctgctc cgcgacaaag gcatggcctg cgttatcctg gcgcataacg agattaaatt
     2821 attttccccg ccggatggag atccgtatga tcgttttcaa atcaaactcc ataaaacagc
     2881 ggcggcgagg cttgaggaat gggccgacgt tgtattgttc gccggatttg ctgtaacggt
     2941 caacgccgac acaggcaaag cgatcaacaa tgccgagcgt gtgatacaca caacaaacaa
     3001 accggcgtgg cgcgcaaaaa cacgctatgt tttaccggac actttaccgc ttaactttgt
     3061 tgaattatta acagctataa aatcaaacca ataaaggaga ataaaaatca tggcaaattt
     3121 acaggggata aacattgacg cgaatgttcc ggaagccgga agttttatcg tagtcccgga
     3181 aggcgtatat aaagcggtca tcgccggaga caagatcgta tccacaaaag acggcaaggg
     3241 gaaaatattg gaattgacca ttcagatcat tgacggatcc cataccggat caacaatcgt
     3301 tgacaggctc aacatcgtca acgccagcca gcaagcccag aacatcgcac agggcacgct
     3361 caaacggatt tgcggggtat tacgcgcccc gttcccccca cagaatacag acagcctgat
     3421 gggtaaaccg atgcttgtca cagtcggcgt tgaagaattt accagcaaca aaacaggaaa
     3481 tgttttgaaa agcaacaaaa tcaaaaacta tgcaccaatc ccgtccgtaa cgtcaactac
     3541 tccggcggcg gtgcagggat ggtaaactca atcattgaca tgattgaggc caagcgagcc
     3601 ggggataaca ttccccggca atacttggga ctttccgaga tcgggcataa atgcccacgg
     3661 tggctgtggt atgcccatca caacacgccg tcaaagccga ttgaaggccg catcatcaga
     3721 ttatttcgga cggggaatat tattgaagat gcgatcatca gcgatctgga atcaatcggc
     3781 attgaagtga cagaccgaca gcgggaagtt gaaatcgtca acggggacat agttttaaag
     3841 gggcatattg acgggatagt atccgggcaa cttttagaaa tcaaaagcgc aggagaaaag
     3901 tatttcaaac aactcttaaa agtcggttat gaaaaatgga atccgaaata tagggctcag
     3961 gcgcacgttt acatggtgct gtgtgacttg gaagagtgca tggtggtagt cgagaacaag
     4021 aatgactcaa acctctatat tgaaacactg aaacttgacc gcgactatgt gaccaaatta
     4081 ttaatcgatg tatttgcagc
//
LOCUS       DAOSME010000128         4069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_23872_length_4069_cov_19.805680, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000128 DAOSME010000000
VERSION     DAOSME010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23872_length_4069_cov_19.805680"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1251
                     /locus_tag="PLG98_11170"
     CDS             229..1251
                     /locus_tag="PLG98_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33868.1"
                     /translation="MNKLCYDIPVLLLHNMNPEWTPEETLETEQDVATLGNALKDLGH
                     PLEIVTVRDDRLAQQLHNFNPRDYVVFNWCEELPGVPHSDPDVAAVMERMGFVFTGSS
                     RDVLALAHDKPEIKRVLDDANIPTPPWAVFDNTDVGEWNIFPSIVKAANEHCSIGISP
                     ESVVTSPKELENRVAFILDKYQQPAIVEQFINGREFLNAVWGNGHISMLPAVEMDYSE
                     LTDLRDRLFSYEAKYVSGSRLYESIDMRVPAELEPSAMAQLENVVIATYRATGCRDYG
                     RIDVRYLNGIFYVIDVNPNPDINPQTSMTHAAAEIGYSYGQMGSRIVNLAAARHPIFS
                     QQYNPV"
     gene            1588..3192
                     /locus_tag="PLG98_11175"
     CDS             1588..3192
                     /locus_tag="PLG98_11175"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOE33869.1"
                     /translation="MAIKDIFVIVSGVILFLIGMVNLSSSIRKLIDVRIKKFIKYAVG
                     RPISGLLTGIVSTIAFQSSSASTILTIGLVSAGLISFSNSLAIILGADIGTTLTVQLV
                     IWRFTDISPLIISLGGLLWLIGRNKWETIGEIIFYFGLMFFGLDLVNKAVEPLKQSPL
                     LIELFTKTQNPLIGIGMGIAVTGIVQASAIPISIMVLLAQQDIVSLENAVAVVMGANI
                     GTTITALLAGTVATRSGKRTAFSHLIFKSTGIGLCFMAMPSFVWILQHISSSVAQQIA
                     LAHFLLNLFIVVIFIFLLSPFAAMMTRFFPGKDDILPVWPEYLDVQDITNPQKALDNV
                     HKELKRQTNMVRDMYLKSMRMINDYSPSDDKSLFYIAMVVKNIREQIVGYLWKLSVQD
                     LSEYYSKNVFAYTAMTSDIESIGNHVLCISKLTAQKTGKNIKFSDCGEVELHEIIDLV
                     SDNFNDAFSVIDKPLDKTIMDVIAREEQVDIKVKEARDNHLERFHKRLCDPEAGPIFV
                     ELLLHLERISDLCNNIAEYMLDVKED"
     gene            3304..3822
                     /locus_tag="PLG98_11180"
     CDS             3304..3822
                     /locus_tag="PLG98_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOE33870.1"
                     /translation="MNKRISALKFALDNELHERDFYLANARRTKNLAGKNMFKQIADE
                     EKEHYDMLKKLFEKWEAKEKWPATIPLKVKKSVAGNIIKAMSEKKGARIKGNEDDLKA
                     IRTAIDFEARGMALYTKLEQGSDDPKEKAFFNLLASIERQHFLSLRDTEEFLTDPASW
                     YQNMEKASLDGG"
     gene            complement(3819..>4069)
                     /locus_tag="PLG98_11185"
     CDS             complement(3819..>4069)
                     /locus_tag="PLG98_11185"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HOE33871.1"
                     /translation="IRAALKKELSDVEKKISGLEDRKTRQETALCDPQTKKDARTIKS
                     INLELKDINRDLEQSYTIWTDLHMKLEQISANAEPFSS"
BASE COUNT         1112 a          960 c          913 g         1084 t
ORIGIN      
        1 tgtataatta ctatatgcaa ataaaagccg gctgtcaatc ccttaagcat atttcaccac
       61 cgtttaaaat atctgaacgg ataagattca tttttccttg aactgtttgg ataaaatctg
      121 ttatagtaaa aacggtcgta aacattttgc atcacaaaaa cgatttcgcc cggtaaaatg
      181 gcatcaacag gcaaaagtcg tttagccgga cgttcagaca ggcgtgttat gaacaaactc
      241 tgttacgaca taccggttct tttgctgcac aacatgaatc ccgaatggac acctgaagaa
      301 actcttgaaa ccgaacagga cgtggcaaca ctcggcaatg ccctgaagga tctgggacat
      361 cccctcgaaa tcgtcacggt gcgggacgat cgtctggccc aacagctcca taacttcaat
      421 ccgcgggatt atgtcgtgtt caactggtgc gaggaacttc ccggcgtgcc gcacagcgat
      481 cccgatgttg ccgcggtcat ggaacgtatg ggctttgtct tcaccggttc ctcccgcgac
      541 gtgctggccc tggcgcatga caagccggaa atcaagcgag tcctggacga cgccaatatt
      601 ccgactccgc catgggcggt ttttgataac accgatgttg gggaatggaa tatttttccg
      661 tccattgtca aagcggccaa tgaacattgc agcatcggta tctctccgga atcggtcgtc
      721 acgtctccga aagaactgga aaatcgtgtg gcgtttattc tggataaata tcaacaaccg
      781 gctattgttg aacaatttat taacggccgc gaatttctca atgccgtctg gggaaatgga
      841 cacatttcca tgttacccgc cgtggaaatg gattacagcg agctgacgga tctgcgggac
      901 cgtctttttt cctatgaggc caaatacgtt tccgggtcgc gtctttatga aagcatcgat
      961 atgcgcgtac ccgcggaact ggagccgtcg gcgatggccc aactcgaaaa tgtggtgatt
     1021 gccacctacc gggctacagg gtgtcgcgat tacggacgca ttgacgtccg ttatttaaac
     1081 ggaatttttt acgttattga tgtcaacccg aatcccgata tcaatccgca aacgtcgatg
     1141 acccatgctg cagcagaaat cggttactcc tacggacaaa tgggaagccg catcgtcaat
     1201 ctggcagcgg cacgccatcc aattttcagt caacagtaca atcccgtata aaacagattt
     1261 tcctcatcgg gaagcatcgc gtcgcatcag aatgataacg catcatatcc gatccgaata
     1321 aaaaacatga tccgttcttt ctgaacgatc ttatgtattc atcattgccc ttgtcttttt
     1381 aagataaatt tgtcaatatt cattcatcaa cacaataacg ttattcaaga aaacgatctt
     1441 gtcaaaaacg caaaagatgc aacacgatct tttcattttg ttaacgatgc tgtgtaaaag
     1501 agaaactgtt caaatcgttt gacggataga ttaaaaaaat gacaacccgg cgccaaacgg
     1561 aaatttaaac ttaaccagga gtaaaatttg gcaataaaag acatttttgt catcgtcagt
     1621 ggcgtcatct tatttctgat cggcatggtc aatctttctt cctccataag gaaattgatc
     1681 gatgtccgca ttaaaaaatt cataaaatat gccgttggca ggccgatttc cggattgctg
     1741 acgggcatcg tttcaacaat tgcttttcaa agctcctccg catcgactat cctgaccatc
     1801 ggactggtca gcgccgggtt aatcagcttc tccaattctc tggccattat tctgggcgcg
     1861 gatatcggca caacattgac cgtccaactg gtcatctggc gcttcacgga tatatcgccg
     1921 ctgatcattt ccttgggcgg attattgtgg ctgatcggca ggaataaatg ggaaacgatc
     1981 ggagagatca tcttttattt cggtctgatg ttctttggtc tggatctggt caacaaggcc
     2041 gtggaaccgc tcaagcaatc ccctctgctc attgagctgt ttacgaaaac acagaatccg
     2101 cttatcggca tcgggatggg aattgccgtc accggcattg tccaggcatc ggccatcccg
     2161 atcagtatca tggttctttt ggctcagcag gatattgtct cgctggaaaa tgccgtggct
     2221 gtggtcatgg gagccaacat cggaactacg ataaccgccc tgctggccgg aacggtcgct
     2281 acgcgcagcg gaaaacgaac ggctttttct cacttgattt ttaaatcaac cgggatcgga
     2341 ttgtgtttca tggcaatgcc ctcttttgtc tggatcctgc aacatatttc gtcaagcgtc
     2401 gcgcagcaga tcgcccttgc ccattttctg ttaaacttgt ttattgtcgt gattttcatt
     2461 tttctgctgt ccccgtttgc cgccatgatg acccggttct tccccggcaa ggacgacatt
     2521 ctccctgtct ggccggaata tctcgacgtt caggacatta ccaatccgca gaaagcactg
     2581 gataacgtcc acaaagaact gaagcgtcaa accaacatgg ttcgcgacat gtatttaaag
     2641 agcatgagaa tgattaacga ttacagtccg tcggacgata aatccctttt ttatatcgcc
     2701 atggtggtga agaatatccg ggaacaaatc gtcggttatc tctggaaatt atccgtacag
     2761 gatctttctg aatattattc taaaaacgtt tttgcatata ccgccatgac cagtgatatt
     2821 gaaagtatcg gcaatcacgt tttatgcatc agcaagctta ccgctcaaaa aaccggtaaa
     2881 aatatcaaat tcagcgactg cggagaagtc gaacttcatg aaatcatcga cctggtcagt
     2941 gataatttta acgacgcttt ttccgttatc gataagccgc tggataaaac catcatggac
     3001 gtcattgccc gtgaggaaca ggtggatatc aaagtcaaag aagcccggga caatcatttg
     3061 gagagattcc acaaacgcct ttgcgatccg gaagccgggc ccatttttgt tgaactgctt
     3121 ttgcacctcg aacgcatttc tgatctttgc aacaacatcg ccgaatatat gcttgacgtg
     3181 aaagaggact gagtcctgag cacatgacct ttaaaacaaa cctattgccc tcatgcgtaa
     3241 tatctcatat aatatgacgt ggagggactg tttacttgga catccgctcg aggaggtatc
     3301 aagatgaata aaagaatcag cgctttaaaa ttcgccctgg acaatgagtt acatgaaaga
     3361 gatttttatc tggctaacgc ccgtcgaaca aaaaatctgg ccgggaaaaa catgtttaaa
     3421 cagattgccg acgaagaaaa agaacactat gatatgctga aaaaactctt cgaaaaatgg
     3481 gaggcgaagg aaaaatggcc ggcgaccatc cctctgaaag tcaaaaaatc tgttgccggt
     3541 aatattatca aagcgatgtc ggagaaaaaa ggggcgcgta tcaaaggcaa cgaagatgac
     3601 ctgaaagcca tacggactgc tattgacttt gaggccagag gcatggctct ctacaccaaa
     3661 ctggagcagg gaagcgatga tcccaaagaa aaagcttttt tcaatctgct cgccagcatc
     3721 gaaagacagc actttctttc gttacgtgac acagaggaat ttctgaccga tccggcgtcc
     3781 tggtatcaga atatggagaa agcatctctt gacggcggtt aagaggaaaa aggctcagcg
     3841 ttcgcggata tttgttccag cttcatatgc aaatccgtcc agatcgtata ggattgttcc
     3901 aggtcgcgat tgatatcttt taattccagg ttgatcgatt ttattgtccg ggcatctttc
     3961 ttagtttgcg gatcacataa tgccgtttcc tgtctggttt tccggtcttc cagtccggat
     4021 atttttttct ccacatcgga cagttccttt ttgagcgccg ctctgatct
//
LOCUS       DAOSME010000129         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_23978_length_4055_cov_16.241500, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000129 DAOSME010000000
VERSION     DAOSME010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23978_length_4055_cov_16.241500"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1139
                     /locus_tag="PLG98_11190"
     CDS             57..1139
                     /locus_tag="PLG98_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOE33872.1"
                     /translation="MNHSKALIFKVIFSSVCIVLVLAGCARYRDIAPGTVKQIPSSSD
                     SAVVSEETVTIPFPDDGTPPAEYVIGPGDVLFVNVSGKPEFSSLNSTSSYSQLGNVQA
                     PSGSRVDGNGNIQMPYLGTIHVGGMSLTQAQKQIQDAAKKYLNDPWVVVEVGQYKSHP
                     LYLLGQFRNSGTFYMDRPMNLLQGVALGNGHDANADLTCARLIRDKKTMPVDINDLLT
                     KGDQRNNIWLRPGDTIFIPDNKNRLVFVFGAVKKGGPVPIPPGGLTLAQAIATAELRD
                     TGHDFQYIRIIRSHSATQGELLVVDFDKILRGESMPMPLHQGDIIFVPKSGLGSWNDA
                     INEILPSLQAFSALLQPFVSIKYLSD"
     gene            1150..3597
                     /locus_tag="PLG98_11195"
     CDS             1150..3597
                     /locus_tag="PLG98_11195"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOE33873.1"
                     /translation="MNTKTPLTPANPAPQQENIFEYIAVIMRRWKIFLTAFCAVFIAV
                     ALYTFLMKPVYEASSMLHIKDSKGNDHILSELSLNTTNPTNAEIEIVKSRSNAEHVVR
                     RLHLNANILKKSDGLSFRILEFKTQARTPVYDITLTGQNTFDVRDDEGDLVGKGTGGV
                     LMKQNGFSLLLDNIKGNPGDSFRLELAPFENVVDAVRNNIKAVEQGRQTGIIKVSYTS
                     TDPVLARDIVNTLVQAYLEQSVSFKSEEASRAVSFIDDQIKSLRGHLDTSEKDLQVYK
                     SSTGVVALDGEANALIEKISGLEQNRTNVNLQRQQIEFAREALKKAMKEDKVYVPGIM
                     MSNPNVETLAAKLADLEVQKKALLTSYTEAHQAVKTVQNQIDEVQNKLLGIYETSRFN
                     LAKQEEAIRSQLSAYEKEMQALPNVERDLARLTRVSKVNSDIYTFLLQKHEEARIAKA
                     STISNIDIVDPAITPGRPVKPKVGQNLLLGLLFGLALGIGLAFFQEYLDDTIKDAEEA
                     KRATGLPLLATIPHIPGYDAPAKNGFTPPKQALITQKEPKSMVAEAFRSLRTNLHFTA
                     IHKEKKIMLFTSTFPREGKSTITANEAVVIAQTGARVLIIDCDLRRSSLHEKFGHSKT
                     PGLTEILTGDAAFDKAKHNTGIPGLDLISAGTTPPNPSELLGSEAMRRFLLAQRENYD
                     FIIIDAPPVLAVTDAPVLATISDIVILIMEAGRVPLKAAQHVRETLATLQASVAGLII
                     NDKTGKGETYGYYGSRYYRYGKGYGYGYGYGYGQDYGYGYYSDEEKKPLRNTRWEKLI
                     NLLPEKLRTQIRNRLRM"
     gene            3699..4049
                     /locus_tag="PLG98_11200"
     CDS             3699..4049
                     /locus_tag="PLG98_11200"
                     /inference="COORDINATES: protein motif:HMM:NF008912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOE33874.1"
                     /translation="MGYRSFEELDVWKLGCNLAVRVYEALKDCKDFGLKDQMTRAAVS
                     ISSNIAEGAERNSKQEFIRFLHIAKGSAAELRTQIYIACKIGIIDNSVQKEFAEELKK
                     ISSMLHSLIKSIKT"
BASE COUNT         1102 a         1213 c          945 g          795 t
ORIGIN      
        1 ttgaacattg aacattgaac atacgttaac catcttacat aaaggaaggc aataccatga
       61 accacagcaa ggcattgatt tttaaagtta tattttcaag cgtctgcatc gttttggtgc
      121 tggccggctg cgcgagatac cgcgatattg cacccggcac tgtcaaacag attccctctt
      181 cgtctgacag cgcggtcgtt tcggaagaaa ccgtcaccat ccccttcccc gacgacggca
      241 caccgccggc ggaatacgtc atcggtcccg gcgacgtcct ttttgtcaac gtcagcggca
      301 aaccggaatt ctccagcctg aattcgacct catcctattc gcaactgggc aatgttcagg
      361 cgccatcggg aagccgggtg gacggcaacg gcaacattca gatgccctat ctcggaacga
      421 ttcatgtggg cgggatgtcc ctgacccagg cgcaaaaaca aatccaggat gcggctaaaa
      481 aatacctcaa cgatccctgg gtggtcgtcg aagtcggaca atacaagagc catccgcttt
      541 acctgctcgg gcagttccgg aactccggca cgttttacat ggaccggccg atgaaccttc
      601 tgcagggtgt cgcgctgggc aacggccatg acgccaacgc cgatctgacc tgcgcccgtc
      661 tcatccggga caaaaaaacc atgccggtgg acatcaacga tcttttgacc aaaggcgatc
      721 agcgcaacaa catctggctc agaccgggcg acaccatctt catccccgac aacaaaaacc
      781 ggctggtctt tgttttcggc gcggtcaaaa aaggagggcc cgttcccatt ccccccggcg
      841 gcctgaccct ggcgcaggcc attgccactg cggaactgcg cgataccgga cacgactttc
      901 agtacatccg catcatccgt tcgcattcag ccacccaggg cgaacttctg gtggttgatt
      961 tcgataaaat tttacgcggc gaatccatgc cgatgccgct tcatcagggc gacatcattt
     1021 ttgttccgaa aagcggattg ggaagctgga acgatgccat caacgaaatc ctgccgtccc
     1081 tgcaggcctt cagcgcactg cttcagcctt ttgtttccat taaatactta agcgattaaa
     1141 aggattatca tgaacacaaa aacaccgctg acacccgcga atcctgctcc ccagcaggaa
     1201 aacatcttcg aatacattgc cgtcatcatg aggcgctgga aaatattcct caccgcgttc
     1261 tgcgccgttt tcattgcagt cgctttgtac accttcctga tgaaaccggt ttacgaagcc
     1321 tcctccatgc tgcacatcaa ggacagcaaa ggcaacgacc acatcctcag cgaactgtcg
     1381 ctcaacacca ccaatccgac caacgcggaa atcgaaatcg tcaaatcccg ctccaatgcc
     1441 gaacacgtag tccggcggct gcacctgaac gcgaatatct tgaaaaaatc ggacggtctt
     1501 tccttccgga tcctcgagtt taaaacccag gcccgaaccc ccgtttacga catcacgctt
     1561 accggtcaaa acacctttga cgtcagagac gacgaaggcg atctcgtcgg caaaggaacc
     1621 ggcggcgtcc tgatgaaaca aaacggcttc agcctgcttc tggacaacat caagggaaat
     1681 cccggcgaca gcttccgttt ggaactcgcc ccgttcgaaa acgtggtgga cgcggtcaga
     1741 aacaacatca aggccgttga acagggccgc cagaccggca tcatcaaagt ctcctacacc
     1801 agcaccgatc cggttctggc gcgcgacatc gtcaacaccc tggtgcaggc ctacctggag
     1861 cagtccgtct ccttcaagag cgaagaagcc agccgggccg tctcgtttat cgacgatcag
     1921 atcaaatcgc tgcgcggcca tctggatacc tccgaaaaag accttcaggt ctacaaaagc
     1981 tccaccggcg ttgtcgccct ggacggcgaa gccaacgccc tgatcgaaaa aatatccggg
     2041 ctggaacaaa accgcaccaa cgtcaacctg cagcggcagc agatcgagtt tgcccgcgaa
     2101 gccctgaaaa aagcgatgaa agaagataaa gtctacgtgc ccggcatcat gatgagcaac
     2161 ccgaacgtag aaaccctggc cgcgaaactg gccgatctgg aagtgcagaa aaaagccctt
     2221 ctgaccagct acaccgaagc gcatcaggcc gtcaaaaccg tgcagaacca gattgacgaa
     2281 gtgcaaaaca aactgctggg catttacgaa accagccgct tcaacctggc caaacaggaa
     2341 gaagccatcc ggagccagtt gagcgcctac gaaaaagaaa tgcaggccct ccccaatgtc
     2401 gaacgcgacc tggcacgcct cacccgtgtt tccaaagtca actccgacat ctacaccttc
     2461 ctcctgcaga aacatgaaga agcccgcatc gccaaggcct ccaccatcag caacatcgac
     2521 attgtcgatc cggccatcac ccccggccgt cccgtcaagc cgaaagtcgg acagaacctg
     2581 ctgctgggac tgctgttcgg actggcgctg ggcatcggcc tggccttctt tcaagaatat
     2641 ctggacgaca ccatcaagga tgccgaagaa gccaaacgcg ccacggggct gccgcttctg
     2701 gcaaccattc ctcatatccc gggttatgat gcgcccgcta aaaacggttt cacaccgccc
     2761 aaacaggccc tgatcaccca gaaagagccc aaatccatgg tggctgaagc attccgctcg
     2821 ctgcgcacca acctgcactt caccgccatc cacaaagaaa agaagatcat gctcttcacc
     2881 agcactttcc cgcgggaagg caaaagcacc atcaccgcca acgaagccgt cgtcatcgcc
     2941 cagacgggcg cccgcgtcct catcatcgac tgcgatttgc gccgttcctc cctgcacgaa
     3001 aaattcggcc acagcaaaac gccggggctg accgaaattc tgaccggcga cgccgccttt
     3061 gacaaagcca aacacaacac cggcataccc ggcctggatc tcatcagcgc cggcacaacc
     3121 ccgcccaacc cttccgaact tctcggatcg gaagccatgc gccggttcct gctcgcccag
     3181 cgggaaaact acgatttcat catcattgac gctccgcccg ttctggccgt caccgacgcg
     3241 ccggtactgg ccacgatatc ggacattgtc attctcatca tggaagccgg gcgcgttccc
     3301 ctgaaagccg cgcagcacgt gcgcgaaacc ctcgccaccc ttcaggcttc cgttgccgga
     3361 ctcatcatca acgacaagac cggcaaagga gaaacctacg gatactacgg cagccgctac
     3421 taccgctacg gaaaaggtta cggatacggc tatggttacg gatacggaca ggactacgga
     3481 tacggctact actccgacga agaaaaaaaa ccgctcagaa acacccgttg ggaaaaactc
     3541 atcaacctcc tcccggaaaa actgagaaca caaatcagaa ataggttaag aatgtgaagg
     3601 atgagaagat agaaagtttt acgttttacg tgttaggtgt taagtaacaa taactgaaga
     3661 ttatttttat tataacgttt acctggatat tttgattgat gggatatcgg tctttcgaag
     3721 aactcgacgt ttggaaatta ggatgcaatc tggctgttcg ggtttacgaa gcactaaaag
     3781 attgtaagga ttttggtctg aaggatcaaa tgacaagagc ggctgttagt atttccagca
     3841 atatcgccga aggtgcagaa cgaaattcca aacaggaatt tatcagattt ttacatatcg
     3901 ccaaaggttc cgccgcagag ctccggactc agatttatat agcctgcaaa attggcatta
     3961 tagataacag cgtccaaaaa gaatttgcag aagagttaaa gaaaatatca tctatgcttc
     4021 acagtttaat caaatctatt aaaacataaa aacag
//
LOCUS       DAOSME010000130         3957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_24757_length_3957_cov_18.633521, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000130 DAOSME010000000
VERSION     DAOSME010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24757_length_3957_cov_18.633521"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1461)
                     /locus_tag="PLG98_11205"
     CDS             complement(1..1461)
                     /locus_tag="PLG98_11205"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE33875.1"
                     /translation="MNSNLTTMWNAMQSGMGNHIPALLGALAVLVLGWLLAVLARAGV
                     RRGLGKLNLNTRFAQLTGQSINLENGLAIAIFWIVMLFIITAVLNSLDLAMVSAPFAA
                     LLTQFLAYIPHLLAGLMLALIAWLLASIVRRFVTKALSKTTLDEKLSEHAGMSPISDG
                     LSQALFWLIILLFVPAIMGALQMDGLLDPFRDMITKVLDFLPNAAAAVAIGFVGWILA
                     TVLRNLTTNLLRTAGSDKLSQKVGLAESVKLSNAIGLLVFVAVFLPALIAALDALKIE
                     AISKPAMDMLDMILMAVPHIVAAGLILVITWMVASFASRLLASLLNAMGFDNIPKHIA
                     LEHAFQKTPASVFVGYVVLFFAMLFATVEAANQLGFTHVSDTVDTFIRFGGDVLLGSL
                     ILVIGFWLANLAYEAMDKASGPNTKGLARIGRFAILGLVLALGLRAMGIADDIVNLAF
                     GLTLGAIAVTVALSFGLGGREPAGKLMEHWLSRFRQ"
     gene            complement(1809..2210)
                     /locus_tag="PLG98_11210"
     CDS             complement(1809..2210)
                     /locus_tag="PLG98_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33876.1"
                     /translation="MKKAFLQSNRVVWRVILPGQCRSLLLVVLIVQSFLLCSCASTPS
                     ESFLKEAAIYSCYGGGSYADTVLNEYDKNTVRLEKFKVTEKYDKKIDDENYTIYKYLI
                     SVHDTKTQLTDNLSCSAAVIKRGPKWYYRLP"
     gene            complement(2778..3053)
                     /locus_tag="PLG98_11215"
     CDS             complement(2778..3053)
                     /locus_tag="PLG98_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33877.1"
                     /translation="MKKTLGTIIVAAAVVLLTATFSFAEYAAAGAANFPFFQLGCLVV
                     GGLILVSLKRKYEKMYIGEVVGVFALYTILMALFTNPVIEVVKTIVT"
     gene            3419..>3957
                     /locus_tag="PLG98_11220"
     CDS             3419..>3957
                     /locus_tag="PLG98_11220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOE33878.1"
                     /translation="MSHYQIAVVVGSLRQDSFNRRLADAIVKLAPPDFSFRQARISDL
                     PLYNQDDDAHQAEAVKRLKNEIKNAHGVLFVTAEYNRSIPGVLKNAIDHASRPYGQNV
                     WAGKPAGVIGISIGVIGTAMSQQHLRNVLAFLDMPTMGQPEVFLQAKDGLFDETGNIG
                     AGSKQFLQDWMDKYVAWVK"
BASE COUNT         1093 a          929 c          880 g         1055 t
ORIGIN      
        1 ttattgacgg aacctcgaca gccagtgttc catgagtttc cctgccggtt cacgtccgcc
       61 cagaccgaag gataacgcca cggtcaccgc aatggcgccc aacgtcaatc cgaacgcgag
      121 gttgacaata tcatcggcaa tgcccatagc acgcagacca agcgccaaaa ccagacccaa
      181 aatggcgaaa cgtccgattc gcgccagccc ctttgtgttg ggtccgctgg ctttgtccat
      241 ggcttcatag gcaaggttgg ccagccagaa tcctatcaca agaataagcg atcccaaaag
      301 cacatcgccg ccgaagcgga tgaatgtatc caccgtatcg ctgacgtgcg tgaatccgag
      361 ctgatttgcg gcttcaaccg tggcgaaaag catggcaaaa aacaagacca cgtatcccac
      421 aaaaaccgat gccggtgttt tttgaaacgc atgttccaat gcgatgtgtt tcggaatgtt
      481 gtcaaagccc attgcattga ggagactagc caaaagccgg gaagcaaaag aagcaaccat
      541 ccaggtgatc accagaataa gaccggccgc aacgatatga ggcacagcca tcaaaatcat
      601 gtccaacata tccattgccg gtttggaaat cgcttctatt tttaatgcgt ccagagcggc
      661 gatcagcgcc ggtaaaaaca ccgccacgaa aaccagcagg cctatcgcgt tggaaagctt
      721 aacggattcc gccaacccga ccttctgact gagtttgtca cttccggcgg tacgcaaaag
      781 attggttgtc agattacgca gaaccgtggc taaaatccaa cccacgaagc caatggcaac
      841 ggcggctgcc gcgttgggca gaaaatcaag cactttggta atcatatccc gaaacggatc
      901 cagaagaccg tccatttgca gtgcgcccat aatggccggt acgaaaagca ggatgatgag
      961 ccagaacaag gcttgactca gtccgtcact gatgggcgac atgcccgcat gttcggaaag
     1021 tttttcatcc agtgtggttt tggaaagcgc cttggtgacg aacctgcgta cgatgcttgc
     1081 cagtaaccag gcaatcagag caagcatcaa cccggccagc agatgcggaa tgtatgccag
     1141 aaattgtgtc aagagcgcgg caaagggggc ggaaaccatc gccagatcaa gcgagtttaa
     1201 cacggccgtt atgataaaaa gcataacgat ccaaaaaata gctattgcca agccattttc
     1261 aaggttgatg ctctgacctg taagttgagc gaaacgcgtg ttcaaattaa gcttgcccag
     1321 tccccggcgg acgcccgccc gtgccaatac ggcaagcaac caccccagaa ccagaacagc
     1381 caatgctccc aaaagggccg gaatgtgatt tcccatgcca ctctgcatgg cattccacat
     1441 cgttgtcaaa tttgaattca tgtttttccc tcctttttat aatatttgtt taataattat
     1501 atttcgggtt gaaaaaacca attaacgact atgtattgat tatctgaagc gttggttgtt
     1561 tcgttgcaaa tatgcgtttt actatttgat gatattgaag cttatttgtg aatgaatata
     1621 ttataaatga aaatacattt ttttaccagc gaaagaatac cgcctgcaag aagcgcataa
     1681 attgtcctgt ttgcattaca taaactgtcc gatgggtcag ttgccttata aaaaaagccg
     1741 gcccaatcat tcttcaattg aaccagctaa aaaaaatgtc aagagtaaaa attttaccgc
     1801 agatccggct acggcaaccg gtaataccat ttaggcccgc gtttaatgac agccgctgaa
     1861 cacgacaggt tgtccgtcaa ctgggttttt gtgtcgtgaa cgcttatcaa atacttgtaa
     1921 atcgtataat tttcatcatc gattttcttg tcgtactttt ccgtaacctt aaatttttcc
     1981 agcctgaccg tatttttatc atattcattc agcactgtat ccgcgtagct tcccccgcca
     2041 tagcaactat aaatcgcggc ttctttgaga aacgattcgg aaggagtcga tgcgcaactg
     2101 cacaataaaa atgattgaac gatcaggaca accaacagca atgacctgca ttgaccgggc
     2161 aaaatcaccc gccacacgac tctattcgat tggaggaatg cttttttcaa tatggcctct
     2221 ttttcaatcc agtgaatttt tatgaagtgt aatctactta tccgccatcc gtcaacagca
     2281 attatttttg atggccggaa agataatatg gattcaaaaa agcatgaggt tttttcttca
     2341 tgtttttctg ctgctcttat tgcaaggcgg ccgtcaatcg tcgtgtccat ctaatgagcc
     2401 tgtggcgtcc tgttcagcca ggtctttttg acttggctgc cttcatcatc ggctatcctt
     2461 ccaggaatgc tctgatagcc ggttgcacgt aaacatcgct caggtcgaat tgccgcaatg
     2521 ccttaattgc aacctgaact ttttctgtta ctgccgttat ggtattgaaa aaccatgcgg
     2581 cggattgatg aaaaggtgat tgacgatatg gaagatgaaa ggatcggatt tcatatcacc
     2641 ccatctcctt ttgaaataaa aaccccctca agctctgcca gccttgaggg ggcgtataac
     2701 aaacagatca tgtgtttgtc gtagcggtta ctttcttgcc gtgttactgt acctcttgtt
     2761 cgtttttgtt gcgtctgtta agtaacgatt gtttttacaa cttcaataac cgggttggtg
     2821 aacagcgcca tcagaatcgt gtagagagcg aagacgccga caacttcacc aatgtacatc
     2881 ttttcatact tccgtttcag ggaaaccaga atcaacccgc caaccactaa acagcccagt
     2941 tggaagaagg ggaaattggc tgcacctgct gccgcatatt cagcaaagct gaaggttgcc
     3001 gttaaaagaa ctaccgctgc cgctacgatg atggttccta atgttttctt catgacctgt
     3061 tacctccttt gggtaatttg ttttatttgc ctttatatta atgaaaatac cgtgccaatt
     3121 tttcagaata gatctataat ttttatatct gcttgattta ttggatgatt ttgccacatt
     3181 tgttgccgac gagaaatcat gaagaagcgt ttatttcatc ttttcttgaa tggaatgttc
     3241 aaaccggtta aacgaaatgt ggaagacaga gaatctatag atgtgtccat ttattttttt
     3301 aaacttttcc taaaaagata ttttagatgt tctttgcatg cgaaatactg taagatgaac
     3361 ttcaataaat tacaaagcat aaacaataag ttatctcttt atacgccggg aggatgtcat
     3421 gagtcattat cagatcgctg ttgttgtcgg aagtcttcgt caagattcat tcaatcgcag
     3481 gctggctgat gctattgtga agttggcgcc gccggatttc tctttccggc aagcgcgaat
     3541 cagcgatttg ccgttataca atcaggatga tgatgcccat caggctgaag ctgtaaagcg
     3601 attgaaaaac gagatcaaaa atgcccacgg cgttttgttt gtcacggctg aatacaaccg
     3661 ttcaattccc ggcgttctca aaaacgccat cgatcacgcc tcccgtcctt acgggcagaa
     3721 cgtctgggca ggcaaaccgg ccggggtgat cgggatttcc atcggcgtta tcggtacggc
     3781 catgtcccag cagcatttgc gaaacgttct tgcttttctg gatatgccga cgatgggtca
     3841 gcctgaagta tttcttcagg caaaggatgg attgtttgat gagacgggta acatcggcgc
     3901 cggcagcaaa caattcctgc aggactggat ggataaatat gttgcctggg tgaaaaa
//
LOCUS       DAOSME010000131         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_26175_length_3799_cov_21.965545, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000131 DAOSME010000000
VERSION     DAOSME010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26175_length_3799_cov_21.965545"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1002..1556
                     /locus_tag="PLG98_11225"
     CDS             1002..1556
                     /locus_tag="PLG98_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOE33879.1"
                     /translation="MKETESRQLTKNEIRSGKVCRKEFHKLKRNPIYLVLDGLKCAHN
                     VGTILRLSDALLVEKVLICGDAALPPSRKVRKGSLGAERWIDWEHRENAFDTVLQLKE
                     KGVFIVTAEIATISIDYRNADYQFPVCLVLGREDRGVSQKIIALSDAVVHLPMYGMVN
                     SLNVSTVAAVLMYDLNNKLNQTAS"
     gene            complement(1992..2438)
                     /locus_tag="PLG98_11230"
     CDS             complement(1992..2438)
                     /locus_tag="PLG98_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33880.1"
                     /translation="MRFQPAKNIALFLSFIALSLIISMPAYCWDRDDNIEKAVRDFKG
                     SYQESGMSLMRSQVSSCYKSALKTKNKKKFEYCAAYDLSAYFLDNQMAQQLNYPPFEG
                     FDLDTASKRIESGLIKLGYSEQQRKSEAKFIGETTAKWFNTVLRTD"
     gene            complement(2445..3464)
                     /locus_tag="PLG98_11235"
     CDS             complement(2445..3464)
                     /locus_tag="PLG98_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33881.1"
                     /translation="MRKIFLVIVVFLLITANAWAVVVTGSGSTKEEAINNGLRDAVEM
                     YTGTLVYGVTDVEDYQVRKDQIVAASLGHVKKYRIIKTSKIDDLVLVTLDVSLAEEKI
                     ERIVRDNVKLLTYEDVLRDYNNVTQRQDQIRKLMDMMRVLAGRPVHEKYGVIYEGYEI
                     KRIGATQVDLILNARVTLNSFYSRAYNEILKHLSLQEDSSDTWLAGGNYRIESGRLTN
                     DRYYIADDMEASTIDDVRAQVHVNGMPVDQCRKYRDNLMVVYKPAAIAGAFVKGFPRA
                     FKQAWNDEPPTTDKKLINVGIKNSKILPPEGLPLKIKYRIQDSKDIKSLWTLKLKMGN
                     CAEGK"
BASE COUNT         1066 a          830 c          874 g         1029 t
ORIGIN      
        1 ataatctgaa tactgaagaa aactactgaa gattgaatcc gtaaactgga tgaccaattg
       61 tttcttgccg gcaatcaact taacaatcac agaaatgaca cggtggccca atttcccata
      121 gtgaaaatga aagcatgaaa tgtttttatg atcggcaaga gccggcgcgt ggaacacaaa
      181 gggaaaatct tattgttctg aagctgacgt cggtccgaga tggagtctta tcaatctctg
      241 aagcaccaaa ttatctgtca tgtacatccg actcatgccg gagctggaaa aatgaatcat
      301 acctggagga tgggggttca attttttctt tttcttacgg ggtaaaatgg agaggtgaaa
      361 cagaaatgga tctggattga aagattatca taaggcaggc aatttagact tttgttgagc
      421 ggaaagaaac aggcgggatg atttgcggta aaaaaaggat gatcaaaagg cggcacgaca
      481 agccatgaat actattccac gctctgtcat ggggtttgcc agctcgccaa tcggcagcat
      541 tcgccctttg atcatccatt ccaatccctt ccatcttcaa aaatgggctc cgggtaagta
      601 gcccgtgagc aaatgccaaa cagcgactga aaccagctca tgaaactgac tcttgccgct
      661 gtagaagatg aaaaggactg gttactgcaa tgggagttga ctagtatcgg cctgattaaa
      721 gtcgctagcc catgacgact gtggaaattt gaacggccga ccgatacaca aactttattc
      781 ccgtaaatat atagttagaa aaattatctc ctgggaaacc gtataagggt tctaatcttt
      841 tggaatgcgg cgcgatctgg cagtgatata ggaataattg cttaaaactt gttatttttg
      901 acgttccaaa acccgccctc aaccaccaac tgacaccaat ctgagattcc ttcgttaagt
      961 atagaaatta ttgagaaaac taaacttaac ggaggtgtca catgaaggaa actgaaagca
     1021 gacaattaac caagaatgag atccgtagcg gcaaggtctg ccggaaagag tttcacaaac
     1081 ttaaaagaaa cccgatctat cttgtactgg atggtctgaa gtgtgcccat aacgtgggaa
     1141 cgatcctccg actgtccgat gcgcttctgg tggagaaggt gttgatctgc ggagacgcag
     1201 cattgccgcc cagcaggaag gtccgcaaag gatcacttgg agcggaacga tggattgact
     1261 gggaacacag agaaaatgct tttgatacgg ttttgcaatt gaaggagaag ggcgttttta
     1321 tcgtcacggc agagattgca accatcagca tcgactaccg gaatgcagat tatcagtttc
     1381 cagtgtgcct ggtactgggg agagaagaca ggggtgtcag tcagaaaatt attgcattat
     1441 ccgatgccgt tgttcacctg cccatgtatg gaatggtcaa ctcattgaat gtatcaacgg
     1501 tagcggcagt gttgatgtat gatctcaata ataagctgaa ccaaacagct tcatgagctg
     1561 atctgaacca tgtaaaaccg gaaaggagta tctgacaatg aagaaaacag tgacgatatg
     1621 gatcatgatt ctatccgcag tattgctcgt ctattggctt tgctttggaa ctgaagcatt
     1681 aagatggctg tatggacctt tggtattggg aatcggcaaa taataaccgt ataaagctcg
     1741 cgccggtaaa agaaataatg gttcggccgg atatgctgtt gatcagtatg tccggccatc
     1801 tatttgtgat tgctgaatct gttttataaa ttaaaatgaa tcgcaggcgc tgctgccaat
     1861 aatgaatgaa aggtgagaat atatctgtac gttttgagat ttcaatactt gtttaaaatg
     1921 caaaagggca tttgttatgg tgtcatggac agatattttt taccggcgag agctgactgt
     1981 gctttcatgc attaatcggt tcgcaagacg gtattgaacc acttcgctgt agtctcgcca
     2041 ataaacttgg cttcactttt gcgttgctgt tcactgtagc ccagttttat caatccggat
     2101 tcaatccttt tggaagctgt atccagatca aatccttcaa atggcgggta attcaactgc
     2161 tgtgccatct gattgtccag aaaatacgct gacaggtcgt aagcggcgca gtattcgaac
     2221 ttctttttat tcttcgtctt cagggcgctt ttataacagc tgctgacctg actgcgcata
     2281 aggctcatgc cgctttcttg ataactgcct ttgaaatccc ggactgcttt ttcaatattg
     2341 tcgtcgcgat cccagcaata cgcaggcatg gagattatca aactcaaagc gatgaagctt
     2401 agaaacagcg cgatattttt cgccggttga aaacgcatac ctgcctattt cccttctgca
     2461 cagttgccca ttttcagctt cagcgtccag agcgacttga tgtcttttga atcctgtatt
     2521 cggtatttga ttttcagcgg caaaccctcc ggaggaagaa tcttcgagtt tttgatcccc
     2581 acgtttatga gctttttgtc ggtggtcggc ggctcgtcat tccatgcctg cttgaaagcc
     2641 ctggggaaac cctttacaaa tgcgccggca atcgcggccg gtttgtaaac caccatcaga
     2701 ttgtcacggt acttcctgca ttgatccacc ggcatgccgt tgacgtgaac ctgagcgcga
     2761 acatcatcga tggtcgaagc ctccatatca tcggcaatgt aatatcggtc attggtgaga
     2821 cggccggact cgatccgata atttccccct gccagccagg tatcagacga gtcttcctgc
     2881 agggagagat gcttaagaat ttcattgtag gccctgctgt agaaggaatt cagcgtaacg
     2941 cgggcattca gaatcaaatc cacctgagtg gcgccgatgc gcttgatctc gtagccttca
     3001 taaatgacgc cgtatttctc atgaaccgga cgtccggcca gaaccctcat catgtccata
     3061 agtttccgga tctggtcctg ccgctgggtc acgttgttgt agtcgcgcag gacatcctcg
     3121 taagtgagca gtttgacatt gtcgcgaaca atcctttcta tcttttcctc cgcgaggctg
     3181 acatccagtg tgacgaggac aaggtcgtca atctttgacg tcttgattat ccggtatttc
     3241 ttcacatggc ccagcgaggc ggcgacgatc tgatctttcc ggacttgata atcttcgaca
     3301 tccgtcaccc cgtaaaccaa agtgcctgtg tacatctcta ccgcatcccg caaaccgtta
     3361 ttgatggctt cttcttttgt cgatcccgat cctgtgacca ccaccgccca ggcgttagca
     3421 gtgatcagta aaaaaacgac tatcacgagg aatatctttc tcataggcat accctcattc
     3481 atcaggaaga aaaatacgct ttggaatatc gccaaaatgc aggcgctaaa acatcaatac
     3541 accgtagggc ggaaaaagta aagaaaaaga tgaatataat cgaatagatg aaaacatttt
     3601 cctatggaaa taagtgattt ctatatgaat atttggcggc ggcgagagcc ggccggtgaa
     3661 acagaaaaag agctaaaaat atttttcaac atttttgttt ttcattttcg accgcttttt
     3721 tcacaaagcg aacaatatca aaagcataag caacattttt taatattcag cgccgttcat
     3781 atagaaattt taattttta
//
LOCUS       DAOSME010000132         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_26278_length_3787_cov_25.943998, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000132 DAOSME010000000
VERSION     DAOSME010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26278_length_3787_cov_25.943998"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1402
                     /locus_tag="PLG98_11240"
     CDS             164..1402
                     /locus_tag="PLG98_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOE33882.1"
                     /translation="MERRVVITGMSAITPIGHGKDQIVQSLLNGTSGIKPLREEEFLS
                     SFIHSKVYGTVDYPVEYDFKRNHRKTMGPVAYYACQVAREVLEQSGLTEDFITSGRLG
                     VAFGSIHGSPTIQRNIYKIFFGAQDKIAVKEIGAVDYLKSMVHTTAVNITKMFGITGR
                     VISSGTACTTSSQSIGYGYESVKFGMQDAMLCGGADEYDTTTVAVFDNLLACSTAFND
                     TPSKTPRPFDKDRDGLVVGEGAGAVMLEEYEHARKRGATILGEVIGFACNNNGGDMIL
                     PNLTGITNTLKLGVETAKINPEEVDLISAHATATKMGDIIEAQAINAVFGYNPHVIGL
                     KSYTGHTMAACGAIETIMALYMMQEGFIAPTLNLENIDERCAMLKHVQKLTDHPVKTA
                     AIQNFAFGGVNTMLMIKKFS"
     gene            1479..2201
                     /locus_tag="PLG98_11245"
     CDS             1479..2201
                     /locus_tag="PLG98_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE33883.1"
                     /translation="MFGYLFILLFLYLPVYVFAKNRATALQNLNHIHLKIFFTLTKRL
                     APKTKYNIPDNLREIHSSVVVCNHLSYLDPILLVSLFKKQKTIVKHTFFKVPIFGWFL
                     KNYGYISSGSDTMMGAAMINNLEDIKQHLSSGGVLFVFPEGTRSRDGSLSPFNKGVFT
                     IARYCNAPLKLILIRGTNKLFQPGHFLFHTQEHNEIELRLIGSLNPDYKSKDFSIAAL
                     ADETRKIFEEKIDHLKSDNKGD"
     gene            2205..2762
                     /locus_tag="PLG98_11250"
     CDS             2205..2762
                     /locus_tag="PLG98_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE33884.1"
                     /translation="MELQEAILKRRSVRKFTDDVVTDEELKQIFEAVRWSPSWANTQT
                     WEFIVVRDKALMEKVAGTYSEKNPATKCSLSASALIVACAKTAISGCYGGKQVTSISN
                     WEFFDLGIAVQTLCLKAHEMGLGTVVVGLMNHEACEKILAVPEGHKVYAVIPIGRPAT
                     EPKAGPPRKSLSEMVFLNNLNKPMF"
     gene            3091..3555
                     /locus_tag="PLG98_11255"
     CDS             3091..3555
                     /locus_tag="PLG98_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE33885.1"
                     /translation="MLNEDYKDILHALSDENVRFLLVGAYALAAHGYPRATMDIDIWV
                     MPSPENADAVLRALQRFGASLQNLTKEDLQKDGTIFQIGVAPRRIDIITAASGLQFEP
                     AYQHSIQLNIEEMEVRIPSIDDLILNKKATGRTKDLADIEILESLKNSEKRL"
BASE COUNT         1071 a          927 c          834 g          955 t
ORIGIN      
        1 gacgccatcg aattgctgct ggctattgaa acctttctga atacggaaat cactcaggaa
       61 gaaaaaaaac aggccatcag tatccgcacg atcaatcaga tttgcgacta cattgtcgaa
      121 atcgcaaaaa aaagaaatct tttcacgccg gcataaaatc attatggaac gcagagttgt
      181 tatcacagga atgtcagcca ttacgccgat cggtcacggg aaagatcaga tcgttcaaag
      241 cctgttaaac ggcacctccg gcatcaaacc gctgcgcgaa gaagaatttc tcagctcctt
      301 tattcattcc aaagtttacg gaacggtgga ttatccggtg gaatacgatt ttaaacggaa
      361 tcaccgcaag accatggggc cggtggccta ttacgcctgt caggtcgcca gggaagttct
      421 ggaacagtcc ggtctgaccg aggattttat cacatccgga agactgggcg tagccttcgg
      481 ttccattcat ggttcgccga ccattcagag aaatatttac aaaatctttt tcggcgctca
      541 ggataaaatc gcggtcaagg aaatcggcgc ggtcgattat ctgaaatcca tggtgcatac
      601 caccgccgtc aatatcacca aaatgttcgg catcaccggc cgggtgatat cgtcgggaac
      661 cgcctgcaca accagttcgc agtcgatcgg ttacggctat gaatccgtca aattcggcat
      721 gcaggatgcg atgctctgcg gcggcgccga cgaatacgac accaccaccg ttgctgtttt
      781 tgataacctg ctggcctgtt ccacggcctt caacgacaca ccgtcgaaaa cgccccgtcc
      841 gtttgacaaa gatcgcgacg gcctggtcgt gggcgaaggc gccggagcgg tcatgctgga
      901 ggaatatgaa catgccagaa agcgcggcgc gactattctg ggagaagtca tcggttttgc
      961 ctgcaacaac aacggcggcg acatgatttt gccgaatctc accggcatca ccaacacgct
     1021 taagctgggt gtggaaaccg ccaaaatcaa tccggaggaa gtcgacttga tcagcgcgca
     1081 cgccacggcc accaagatgg gcgacattat tgaagcgcag gccatcaacg ccgtcttcgg
     1141 ttataatcct cacgtcatcg ggttgaaaag ctacaccggt cacaccatgg ccgcctgcgg
     1201 cgccattgaa accatcatgg ccctctacat gatgcaggaa ggctttattg cgccgacgtt
     1261 gaatctggaa aatatcgatg aacgctgtgc catgttaaaa catgtacaaa aattaaccga
     1321 tcatccggta aaaacagcgg ccattcaaaa tttcgctttt ggcggcgtca atactatgct
     1381 gatgattaaa aagttttcct gagttgaaga tgtttctgtc ccgtattttt cattttgcag
     1441 ttaatttatc cgtaacactg attttgtggt gctatttcat gttcggatac ctttttattt
     1501 tactgttttt atatctgccc gtttacgttt ttgcaaaaaa ccgcgctact gctcttcaga
     1561 atttaaacca tattcattta aaaatattct tcacgttgac gaaacggctt gcgcctaaaa
     1621 caaaatataa tattcccgat aatttgcggg aaattcattc atcggttgtt gtctgcaacc
     1681 atttgtccta tctcgatccc atcctgctgg tgtccctttt caaaaaacag aaaaccatcg
     1741 tcaagcatac ttttttcaaa gttcccatct tcggctggtt cttgaaaaac tacgggtata
     1801 tttcctccgg atccgatacc atgatgggcg cggccatgat caacaacctg gaagatatca
     1861 aacagcatct gtcgtcgggc ggcgttttat ttgtttttcc ggagggcacc cggagccgcg
     1921 acggcagtct ttcgccgttc aacaaaggcg tctttacgat tgcccgttat tgcaacgcgc
     1981 cattgaaact cattttaatc cggggaacca acaaactgtt tcaacccggt catttccttt
     2041 tccacactca agaacataat gaaattgaac tccggttaat cggttcgctc aatccggatt
     2101 acaaaagcaa agatttttcc atcgctgccc tggctgacga gaccagaaaa atattcgaag
     2161 aaaaaataga ccatttaaaa tcagataaca aaggggacta ggttatggaa cttcaagaag
     2221 ccatcttaaa acgcagaagc gtcagaaaat ttaccgacga tgttgtcacc gatgaagaac
     2281 tcaaacaaat ctttgaggcc gtacgctggt cgccctcctg ggccaatact cagacttggg
     2341 aattcatcgt cgtccgcgac aaagcgctga tggaaaaagt cgccggcacc tattccgaaa
     2401 aaaacccggc cacaaaatgt tctctaagtg cgtccgctct aattgtcgcc tgcgcgaaaa
     2461 cagccatttc cggttgctac ggcgggaaac aggtcacttc aatttccaac tgggaatttt
     2521 tcgatctggg catcgccgtt cagacgcttt gcctgaaagc ccatgaaatg ggacttggaa
     2581 ccgtggttgt gggtttaatg aaccacgaag cctgtgaaaa aattctggcc gtgcccgaag
     2641 gtcacaaagt ttatgccgtc attccgatcg gccgaccggc caccgaaccc aaggcaggac
     2701 cgccgcgcaa atccctttcc gaaatggtat ttttgaataa cctcaataag ccgatgttct
     2761 aaatcattcc aacccaccgg cagggagcta tctccctgcc tttccaaaaa gcgtgtaact
     2821 tcagcttccg gctgcgttat tcataaatga agatataaaa aatagcaaag tgtccccaga
     2881 ttttcgaaat tttcggaacc ggccacaggg aatatccggt tgattttact tataaaaaat
     2941 ggtaatttaa atttatgaat aaaaagcaaa ccgccataca ccgaatgacg gatcatcagg
     3001 acgacaatta tgtccccggc acgccggaag agcggattct gcttgtttgg ccgttgacac
     3061 gtgaaatatc ctccctgagt ataaagcatc atgctgaacg aagactacaa agacatttta
     3121 cacgccttat ccgacgagaa cgttaggttc cttcttgtcg gagcgtatgc acttgcggct
     3181 catggttatc cccgggccac gatggatatc gatatctggg tcatgccatc tccggaaaac
     3241 gctgatgcgg ttctgcgggc tttgcaacgt tttggcgctt cattgcagaa tttaaccaaa
     3301 gaagaccttc agaaggacgg aacaattttt caaatcggag ttgccccaag acggattgac
     3361 attatcaccg cggcctcggg gctccagttc gaacctgctt atcaacactc aattcagctg
     3421 aatatagagg aaatggaagt ccgtattcct tcgatcgacg accttatcct caataaaaaa
     3481 gcaacaggaa gaacaaaaga tcttgccgat attgaaatct tggaatcttt aaaaaattcc
     3541 gaaaaacgtc tttgaaacaa atgcacaaat aattctgata ctggtttagt cgtccttcga
     3601 ttgaagccac tatgaatctc gataagccga tgtgttaata tttaatttaa agcgatcatc
     3661 cgcaaaatac ccagcgatga tgaaaatatt ttcaaaataa gaaggggaag ttgaatgtta
     3721 tgaaagcctc ttttatccgc aaagttctga tcatgatcgt cagtctgtat gtgacgctga
     3781 gaattac
//
LOCUS       DAOSME010000133         3771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_26430_length_3771_cov_31.652583, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000133 DAOSME010000000
VERSION     DAOSME010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26430_length_3771_cov_31.652583"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1063
                     /locus_tag="PLG98_11260"
     CDS             113..1063
                     /locus_tag="PLG98_11260"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOE33886.1"
                     /translation="MDTRRDFIKKSVVVCAALSLPLQGAANAQTVQREMKTRDPKRAL
                     VVSYSQSGFSARYGKLIACLLKDQGLAVELADMRRFDSKRLPDYDLILVGSPVFYYDI
                     PENVVDWLSSAPKLTGIPVAAFVSFGGPEGNQHNALCHALHLLAEKEAVPVGMEAFRS
                     IPSYPGSWDSANQRSGEHLPDEATYNRVREFAKQIPEKVRQGQSFSYSSEIALREIVR
                     MLPLVWLNKKAIGKHTVDGAKCIGCRTCVKMCPVGAIHPERQFVDQDKCIACFGCLNN
                     CPADAVVVEYGGKKLYGFPEYLKRRKITILEPREFQHCKL"
     gene            complement(1305..1481)
                     /locus_tag="PLG98_11265"
     CDS             complement(1305..1481)
                     /locus_tag="PLG98_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33887.1"
                     /translation="MLRYQIWSTTKGMLIAVSAPDAAGNINVQQAILMGAQVAKQALN
                     VELSSLSSALFVRN"
     gene            complement(1554..1781)
                     /locus_tag="PLG98_11270"
     CDS             complement(1554..1781)
                     /locus_tag="PLG98_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOE33888.1"
                     /translation="MKKIKLHVELTKAEEGGYVVYCPELDITTEGETVEEAMAMIRDA
                     AAGYIDVVGFDNIPAFSGKILKEELEMVVNG"
     gene            complement(1916..2305)
                     /locus_tag="PLG98_11275"
     CDS             complement(1916..2305)
                     /locus_tag="PLG98_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE33889.1"
                     /translation="MNEPSKIYQELAEENYLLRQKVQELEIIAGERRKAEKGLRDSEN
                     KYKILTEKMSDIAWIADMNLKTLYVTPSIQTVLGFSQEERIFQNITEQLTPDSLSRVM
                     MIESFVSTPRSHAPHGIAVLARRACMS"
     gene            complement(2302..3006)
                     /locus_tag="PLG98_11280"
     CDS             complement(2302..3006)
                     /locus_tag="PLG98_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOE33890.1"
                     /translation="MERLGVLSGTFMLERSDWIQKGKKHRIQNAWGEATAVETDSIIF
                     ILRHGDDPADYILPHRVNHPANLQALKDLGVTEVVGINSTGSLKRNLQPGMIVIPDDF
                     IVFTPTPTIFHSEPIHVAPSFNEPVRQKLIAAARRIDLNVVETGIYWQTTGPRLETRA
                     EIRMMSGFADIVGMTLASEAVIALEMDLPYASACSIDNFGNGLVEKPLSLEEIIAGTR
                     NNADLMIRLLEEYVHR"
     gene            3159..3488
                     /gene="trxA"
                     /locus_tag="PLG98_11285"
     CDS             3159..3488
                     /gene="trxA"
                     /locus_tag="PLG98_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE33891.1"
                     /translation="MSGQFLGVATDANFEQEVLKSDKPVLIDFWAPWCGPCKAIGPLV
                     EEIAGEYANRVKVMKLNVDESQKSAVAFGVRSIPTLILFKDGKVLDTLVGMTPKEKLE
                     EFVKKGL"
     gene            3548..>3771
                     /locus_tag="PLG98_11290"
     CDS             3548..>3771
                     /locus_tag="PLG98_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33892.1"
                     /translation="MKLKYAGVILVVLLICTGCSFNMATIKSFFVKSDPVRSTPEGLY
                     SRGTAEYQNGSYKKAREYYTRLKEEYPLHNL"
BASE COUNT          883 a          959 c          997 g          932 t
ORIGIN      
        1 gctatccgat gaccacgcat ctggacatgg gagtctatga gttgaaaatc aattttggcg
       61 gtgaagaaaa gaaagaataa tcagacgctg cggaaaggct ggaaatgatt ctatggacac
      121 cagaagagac ttcatcaaaa aaagcgtggt cgtctgcgcc gctttgagcc tgccgttgca
      181 aggggccgcg aacgcacaga ccgtccaacg ggaaatgaaa acccgcgatc cgaaacgcgc
      241 gctggttgtg agctacagcc agtcgggatt ttccgcgcgc tacggaaagc tgatcgcctg
      301 cctgctgaag gatcaggggt tggcggttga attggccgac atgcgccgct tcgattcaaa
      361 gcgcctgccg gattatgatt tgattctggt cggctctccg gttttctatt acgatattcc
      421 ggaaaacgtg gtggactggt tgtcttccgc gccgaagctg acggggattc ccgtggcggc
      481 gtttgtgtcc tttggcggtc cggaaggcaa tcagcacaac gcgctttgcc atgcgcttca
      541 tctgctggcg gaaaaggaag ccgtacccgt cggcatggag gccttccgca gcattccgtc
      601 ctatccggga agctgggaca gcgccaatca acgctccggc gagcatctgc ccgatgaggc
      661 gacctacaac cgggtgcggg agtttgccaa acagattccg gaaaaagtca ggcagggcca
      721 atctttttcc tattcctccg aaattgcgct tcgggaaatc gtgcggatgt tgcctttggt
      781 ctggctcaac aaaaaagcca tcggcaaaca tacggtggac ggcgccaaat gcatcggctg
      841 ccggacttgc gtgaaaatgt gtccggtcgg cgcgattcat ccggagaggc agtttgtgga
      901 tcaagataaa tgcattgcct gtttcggctg tctgaacaac tgcccggcgg atgccgtggt
      961 ggtggaatac ggcggcaaaa aactttacgg gtttccggag tatctcaaac gcagaaagat
     1021 caccatcctg gagccgcggg agttccagca ttgcaaactt tgatgaaacc gtaaaaaatc
     1081 ataatttact actaattccc tctcccttga gggagggcct ccacaacttc ccttctgcgt
     1141 cttttaatcg cccctcccct tgtgggaggg gcggggggag ggggtcaatt aaactcgtat
     1201 cttccgaatt aagctgactc atttttcacc ctcaccctaa ccctctcccc tcaagggaga
     1261 gggaagtaaa ggatgtccca cttcctcttt tatctccccg cgcctcaatt cctcacaaac
     1321 agagccgagg acagagagct cagttccaca ttgagcgcct gcttggccac ttgtgcgccc
     1381 atcaggattg cctgctggac attgatgttg ccggccgcgt ccggagcgga aaccgcaatc
     1441 aacattcctt ttgttgtcga ccagatctga tagcgcagca tgaaccgtaa aatgtagatc
     1501 agagtatcag tgatagcaaa aaggaaagtc ttccttttga aattcccgct ttcttatcca
     1561 ttaaccacca tttccagttc ttctttcaaa atcttaccgg aaaaagccgg aatgttatca
     1621 aaacctacaa catcaatata accggcggcg gcatccctta tcatggccat ggcctcctcc
     1681 acggtttcac cttccgtggt tatgtcaagc tccgggcagt aaactacata tccaccttct
     1741 tctgccttgg tcagttcaac atgcaatttt atttttttca tcgccaaaat cggacctcca
     1801 tgatttccgt caatgggttc tatacgttga tatggacttc tttgtttctg aaaataagca
     1861 ataactttat aagtgtcaat gcattttttg gtctggttca atcttccgaa ttgagctaac
     1921 tcatacaggc gcggcgcgcc aagactgcaa tcccatgcgg cgcgtgggaa cgaggggtag
     1981 aaacgaaaga ttcaatcatc atcaccctgg acaaggaatc gggcgtcaac tgctctgtaa
     2041 tgttctgaaa aattctttcc tcctgactga atccgagcac ggtttggatg gatggcgtga
     2101 catagagggt tttcagattc atgtccgcta tccaggcgat atcggacatt ttttcggtaa
     2161 gaatcttgta tttgttttca ctgtcgcgga ggcccttttc agcttttctt cgttctccgg
     2221 ctatgatctc cagttcctga actttttgcc ttaacaggta attttcttcg gccagttcct
     2281 gatagatttt ggatggttcg ttcatcgatg aacatactcc tcgagcaggc ggatcatcag
     2341 gtccgcgttg ttgcgcgttc cggcgatgat ttcctccagg ctcaaaggtt tttccaccag
     2401 tccgtttccg aaattgtcga tggagcaggc ggatgcgtag ggcagatcca tttccagggc
     2461 aatgaccgcc tcgctggcca gggtcattcc gacgatgtcg gcaaaaccgg acatcatgcg
     2521 gatttcggcc cgcgtctcca ggcgggggcc cgtggtctgc cagtagattc ctgtttccac
     2581 aacgttcaga tcgattctcc gcgccgccgc aatcagtttt tggcggacgg gttcattgaa
     2641 agaaggggcg acatggatcg gctcgctgtg aaaaatggtg ggggtcggcg tgaaaacgat
     2701 aaaatcatcg gggatgacga tcatgcccgg ctgcaggttt cttttcaggg atccggtcga
     2761 attgatgccg accacctcgg tgacgccgag atccttcagc gcctgaagat tggcggggtg
     2821 attgacccgg tggggtaaga tgtaatccgc cggatcatcg ccgtgtcgaa ggataaagat
     2881 gatggagtcc gtttcgacgg ccgttgcctc accccaggcg ttttggatcc ggtgcttttt
     2941 tcctttttgg atccaatcgc ttcgctccag catgaatgtt cccgacaaga cgcccaaacg
     3001 ttccatacga aacctcccca tgtgtgcttt tttatagcag ataaaccgga cggcgtcaaa
     3061 agaatcaggg cggctcgagg cggcggtcaa atagcatttg actttttgaa acccgctgtg
     3121 acatataatc gcgaaatcaa caaaggaggt tgcgaatcat gagcggccaa tttctgggtg
     3181 tggcgacgga tgcaaatttt gaacaggaag tcctgaaaag cgacaagccg gtcctcattg
     3241 atttctgggc gccctggtgc ggaccgtgca aggccatcgg ccctcttgtg gaagaaatcg
     3301 ccggcgaata tgcaaaccgg gtcaaggtca tgaagctgaa tgtggacgag agccagaagt
     3361 cggccgtcgc cttcggcgtt cgcagcattc cgaccctgat tttgttcaag gacgggaaag
     3421 tgctggacac gctggtcggc atgacgccga aggaaaagct cgaagagttt gtcaagaaag
     3481 gcctgtgaag gctttcggcc cagaccgcaa aggtaaaaaa tttattttgg atgaaacggg
     3541 tatccttgtg aagctaaaat atgcaggcgt cattctggtt gtgctcttga tctgtaccgg
     3601 ctgctctttt aacatggcga ccatcaagtc gttttttgta aagagcgatc cggtccggtc
     3661 aacccccgaa ggcctgtatt cgcggggcac ggccgaatat cagaacggct cttacaaaaa
     3721 ggccagggaa tactataccc ggctcaaaga ggaatatccg ctgcacaatc t
//
LOCUS       DAOSME010000134         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_26464_length_3768_cov_25.501481, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000134 DAOSME010000000
VERSION     DAOSME010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26464_length_3768_cov_25.501481"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..871
                     /locus_tag="PLG98_11295"
     CDS             200..871
                     /locus_tag="PLG98_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE33893.1"
                     /translation="MPKKPRTKEEIENIREKILDTALHIIIEEGFNNLSLRKIASRLN
                     VTATTIYNYYTNKDEINLMIRIRGFEKLHDLLIARSAALKKIEDRLKAMIRAYIEFGL
                     TYPSYYDIMFNLHTPKYLDYAGTEIEPLAFREKQNALKCLTLFIDPVTSYIPIKGKKK
                     EHFILYQVIKFWSDLHGLITLNNSRLFHEVTDDVDDFIAKRTADIVDNFLKLKDSLDE
                     GKKIF"
     gene            905..>3768
                     /locus_tag="PLG98_11300"
     CDS             905..>3768
                     /locus_tag="PLG98_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOE33894.1"
                     /translation="MQNVQPVQKSYANTLARMFFKFVALNFNNRPSLNRYLKSDDGWI
                     NFTFGIKTENGSVEQAMRFENGKVTVLKNIPETLDSQLIFVDENALKEAATQPPNKLM
                     LALMENRMITIGNLGYLQLINLYLSLLVKQIQIAKLKKEAKTENRKYDAAKASRYCIE
                     ARKPRQLKAVNVDPGVRFLNDPYLAQYSLDDFPRVKEFLDIHLKIKPAICHERAELVT
                     QWFKENGFEKDKNGKPWVSELRQAQAFQYLMANKKPIIRKNDLIAGTTTTKDIGVIIY
                     PEAHGTMIWGELLTVPYRPLNPYNIDADTLDVLHNKVFPFWLKRNFREWVRDHKDYPL
                     CQKLDERFAACFLWKTVALSHTILNYPEVLSLGTSGIISKIDKELSETPESEKQKRDT
                     LQAMKISLEGMNAYAGNLAQEAWRLASQETQSVRKNELLHLAEICKRVPAHPCTTLDE
                     AVNALWIIWVGVHMENTNAGFSLGRLDQWLQPYFTADMKKIQDEKERQEYVKHAIELI
                     GCFYMRCTDHLPLIPDIGNYLFGGSSSDQAITLGGVTPDGEDAVNDMTYIFLKVTEML
                     SIRDPNVNARYNKSKNSATYLKRLCEVNINTTATPSIHNDEAVMASLAEFNYPPEHLR
                     DWSATGCVEPTLSGRHIGHTNCMMFNMVAALEMALNNGRHPLMRWDLGPKTGDIRAGA
                     FPDFESFFNAFAAQLRFLADQTCEYNNLLGEAHQHIRPTPFISALIEGPLKKGKDVTK
                     GGARYNSSGTACIGLADITDSLMVVKKLVFEEKKITFERLLDAVNTNFRNDSALLAQI
                     KNKIALFGSGNEEALATANRVIKLAHDIFGEHTNYRNGKYTAGFWSMSNHVAFGTLTG
                     ALPSGRLAGKAFTPGLTPEPHASKNILDNIRDVAKLKAENMNNNIAFNVKVVPGAQES
                     HQKVVDNIYSYVKTYSDLGGMQMQLNVVSSKTLRDAMEHP"
BASE COUNT         1098 a          950 c          849 g          871 t
ORIGIN      
        1 cggactctga ctccgtttat taaggttcga atccttattc cccagccata acttcaccat
       61 ttaatatcag ttatttcaat attttacatg aatattatgc ggccgggcaa tttctgccgg
      121 tcccgttgat attgcgcttg actttttaaa ataacactgt taagttaaca tcgtaatatt
      181 taaaaataag aatcaaatta tgcccaaaaa accgagaacg aaagaagaaa tagaaaatat
      241 tcgggagaag atcctggata cggctcttca cattattatt gaggaaggat ttaataatct
      301 cagtcttcgc aaaatagctt cccgtttgaa tgtcacggcc acgaccatct acaattatta
      361 taccaataaa gatgaaatca atctgatgat cagaattaga ggctttgaga aacttcacga
      421 cctcctgatc gcacgctcgg ctgccttgaa gaaaatagaa gacagattaa aagccatgat
      481 ccgtgcctac atagaattcg gcctcaccta tcccagctac tacgacatca tgttcaatct
      541 tcataccccc aagtatctgg actatgcggg aacggaaatc gaaccgctcg cctttcggga
      601 aaagcagaat gcgttaaaat gtctcacgct ctttattgac cccgtcacgt catacattcc
      661 catcaaggga aagaaaaagg agcattttat tttatatcag gtaattaaat tctggtctga
      721 ccttcacggc cttatcacgt tgaacaacag ccgcctcttt cacgaagtaa cggatgatgt
      781 ggatgacttt attgcaaaac gaacggcgga catcgtagac aatttcctga aactgaagga
      841 cagcctggat gaaggaaaga aaatttttta actgatcatt aaacccgtta ttcggaggtc
      901 tattatgcaa aatgtccagc ctgtgcaaaa aagttatgcc aacacccttg cccgcatgtt
      961 tttcaaattc gttgccttga atttcaacaa ccgtccgtcg ctgaaccgat atctgaaaag
     1021 cgacgacggc tggattaatt ttacattcgg catcaaaacg gaaaacggca gcgtcgagca
     1081 ggccatgcgt ttcgaaaacg gcaaggttac cgtgttgaaa aatattccgg aaaccctgga
     1141 ttcgcaattg atattcgtgg atgaaaacgc ccttaaggaa gccgccacgc aaccgcccaa
     1201 caaattgatg ttggcgctca tggaaaacag aatgatcacg attgggaacc tcggctatct
     1261 gcagttgatt aatttgtatc tttcgctcct tgtgaaacag atccagattg caaaactcaa
     1321 gaaagaagcc aagacagaaa accggaaata cgatgcagcc aaagcatccc gatactgtat
     1381 cgaagccaga aaaccccgcc agctcaaggc cgtcaacgtt gatccgggcg tccggtttct
     1441 aaacgacccc tatctggccc aatattccct tgatgatttt ccccgggtca aagaatttct
     1501 cgacatacac ctgaaaataa aaccggccat atgccacgaa cgggcggaac ttgtcacaca
     1561 atggtttaaa gaaaacggat ttgaaaaaga taaaaacggc aaaccctggg tttccgaact
     1621 gcgccaggcg caggcttttc aatatctgat ggcaaacaaa aaaccaatca tcaggaaaaa
     1681 tgatctgatc gccggaacaa ccaccacgaa agacattggc gttattatct atccggaggc
     1741 gcacggcacc atgatctggg gagagttgct cacagttcca tacaggccgt tgaacccgta
     1801 taatattgat gccgatacgc ttgacgttct gcacaacaaa gtctttccgt tctggctcaa
     1861 gcgcaacttc cgcgagtggg tgcgggacca taaagattat cctctttgcc agaagcttga
     1921 tgagcgtttt gccgcctgct ttttatggaa aaccgtcgcg ctgtcgcata cgattttaaa
     1981 ttatcctgag gtcttaagcc tgggcaccag cggtattatc tcgaagatag ataaagaatt
     2041 aagcgaaaca cccgaatcag aaaaacaaaa acgtgacacg ctgcaagcca tgaaaatcag
     2101 tcttgaggga atgaatgcct acgcgggtaa tctcgcgcaa gaagcgtggc ggctggcgtc
     2161 tcaggaaact cagtctgtcc gcaaaaacga actgctgcat ctggccgaaa tctgcaagcg
     2221 cgttccggcc catccctgca caacgctgga tgaagcggtc aatgccctgt ggatcatctg
     2281 ggtcggcgtc cacatggaaa acacgaacgc cggattttct ctcggaagac tggatcaatg
     2341 gcttcagcct tattttacgg ccgacatgaa aaaaattcag gacgaaaaag aaagacagga
     2401 atatgtcaag catgcgatag aattgattgg atgtttttac atgcgctgca cagaccacct
     2461 gcccctgatt ccggacatcg gcaattatct gttcggcggc tcctcctccg atcaggccat
     2521 tacgctgggc ggcgtaacac ccgacggcga agacgccgtc aacgatatga cctacatttt
     2581 tctgaaagtg acggaaatgc tttccatccg cgatcccaac gtcaatgccc gttacaacaa
     2641 gagcaaaaac agcgcgacct acttgaaaag gctttgcgaa gtcaatatca acacaacggc
     2701 aaccccgtcc attcataatg atgaggcggt catggcctcg cttgcggaat tcaattatcc
     2761 gccggaacat ttacgcgact ggtcggcaac cggctgcgtg gaaccgacct tgtccggacg
     2821 gcatatcggt cacaccaact gcatgatgtt taatatggtg gccgcgttgg aaatggccct
     2881 caacaacggc cggcatccgt tgatgcgctg ggatctggga ccgaaaacag gcgacatccg
     2941 cgccggcgca ttccccgatt ttgaatcctt cttcaacgcg tttgcagcgc agctccgttt
     3001 tctggccgat caaacctgtg aatacaacaa tctgctgggc gaagctcacc aacacatccg
     3061 cccgactcct tttatctcgg cgctgatcga aggtccgttg aaaaaaggaa aagacgtaac
     3121 caagggcggg gcccgttaca attcatccgg aacggcctgc atcggccttg ccgacattac
     3181 cgactcgctg atggtggtca aaaagctggt ttttgaagag aagaagataa cgtttgagcg
     3241 gctgctggat gcggtcaata caaatttcag aaacgactcc gctctgctgg ctcagattaa
     3301 aaacaagatt gctcttttcg gatcaggcaa tgaagaggcg ctggcaacag ccaatcgcgt
     3361 catcaaactg gctcacgata ttttcggaga acacacaaat taccgcaacg gcaaatacac
     3421 agccggtttc tggtcgatgt ccaatcacgt tgctttcggt acgctgaccg gcgcgcttcc
     3481 gtcaggacgg ctggccggca aagccttcac accgggtctc accccggaac cccatgcgtc
     3541 gaagaatatt ctggacaaca tccgggatgt ggccaaactc aaagcggaaa acatgaacaa
     3601 caatatcgct ttcaatgtca aagtcgtccc cggtgcacag gagtcgcatc agaaagtcgt
     3661 ggataatatt tattcctacg tcaaaaccta cagcgatctg ggcggtatgc agatgcagtt
     3721 gaacgtcgtg tcgtcaaaaa cgcttcgcga cgccatggaa catccgga
//
LOCUS       DAOSME010000135         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_26530_length_3761_cov_19.784404, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000135 DAOSME010000000
VERSION     DAOSME010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26530_length_3761_cov_19.784404"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1141
                     /locus_tag="PLG98_11305"
     CDS             386..1141
                     /locus_tag="PLG98_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE33895.1"
                     /translation="MLTLNKVFFTAPGGDGENSAPRNIIDGLDFTFEDGKFYAITGPN
                     GSGKTTLAKLIMGINPITQGSIVFNGQDISGLSITDRAKLGIAYSFQQPARFKGISFR
                     DLLSIATGTDEEKKFLEIVSRVGICPLSFLDKEVDSKLSGGEIKKIELATTIATNPKL
                     AIYDEPDTGIDLWTIGPMVSLLKKELTPKTTTIVVSHNKAFLDVADVIIILNQGRIAY
                     TGNLKDALPLLNDMSICAYHNKLEGFENAECFR"
     gene            1122..2042
                     /locus_tag="PLG98_11310"
     CDS             1122..2042
                     /locus_tag="PLG98_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOE33896.1"
                     /translation="MLNALDKKLLKTVADLESLPKGAYNIRKNGKLLHREVSANINIE
                     TNADGTGIIVTIKPGTKNESVHIPVILSQAGLYDVVYNSFIIGEGAEVTIVAGCGIHC
                     GSSEAEGHAGIHDFHVGKKAKVTYVEKHFAMGEGKGRRSLNPTTKVFLDEGATAEMEL
                     TQISGVDEANRLNEAELGPDSLLLIMERVLTEGEQSAVSTNNIILKGFGSRANMISRS
                     VMKGNSKQIFHANMEARNKCFGHIECDAIIMDNGTNETMPALKALHPDAELTHEASIG
                     KIANDQLTKLMSLGLTYDQAINRIIQGFLK"
     gene            2077..3159
                     /gene="rfbB"
                     /locus_tag="PLG98_11315"
     CDS             2077..3159
                     /gene="rfbB"
                     /locus_tag="PLG98_11315"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOE33897.1"
                     /translation="MTKNIHPPVTLHNLLVTGGCGFIGSNFIRYLIDRIHHSGRIINV
                     DKLTYAGNPENLAEIAANGASRYIFQKADICDRARLEEIFSQYDIDGVCHFAAESHVD
                     RSIKTPGDFIQTNIVGTFNLLECAKARGDRFKIFHHVSTDEVYGSLGADGLFREDTPY
                     RPNSPYSASKAASDHLVRAYYKTFGLPVTISNCSNNYGPYQFPEKLIPLIVLNALEGK
                     PLPVYGDGKNVRDWLYVEDHCEAIWTIMTSGRHGETYNVGGKAEMENIAIVQLICDIL
                     DDIQPASDNSPRRRLITFVKDRPGHDRRYAIDFSKLQKELNWAPKESFSSGLQKTVNW
                     YLDQKKWVENIKTGAYLNWIKEQYGA"
     gene            complement(3211..3627)
                     /gene="rnk"
                     /locus_tag="PLG98_11320"
     CDS             complement(3211..3627)
                     /gene="rnk"
                     /locus_tag="PLG98_11320"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOE33898.1"
                     /translation="MADRTIYITKFDMDRLLELIDGVRSTPRHNKANIDLLEKELCNG
                     TVVDSEDVPADVITMNSRVSVTDIESGEQMTYTLVFPSEANIAENKLSILVPLGMALI
                     GYRKGDIIEWPVPSGVRKIKVNDVIYQPEAAGNYNL"
BASE COUNT         1066 a          896 c          861 g          938 t
ORIGIN      
        1 aaagaaagca gctcccaaga aaaaagtggc aaagaaagca gcaaagaaga aataaagaaa
       61 atttcattta aacttttttt gacgaagggg gcataacggt gcaataattt tcccctgaaa
      121 aaggaaaatg ctttttgtcg ttgtgcctcg aaagaaaaat caggcggcat ttgttttcaa
      181 aacatctgcc gcctgatttt atttggccca tcacccatgt ttcttcaatt ccactcctga
      241 aaaataccgg cagggatgta tcatttttca gcgttgcttt ttagacgaaa ttgtgtctat
      301 agtgccaccg aaaattgtga tattttcaac agttgatggt gttcatatat tttttaatat
      361 tttaagataa gggcaggttc aacgaatgtt aacattaaat aaggtttttt ttaccgctcc
      421 cggcggcgat ggtgaaaaca gtgccccacg caatatcatt gacggcctgg attttacctt
      481 tgaagacgga aaattttacg ccattacggg ccccaacggc agcggaaaga caacgctggc
      541 caaactgatc atgggcatta atccgatcac acaaggctcc attgttttta acggccagga
      601 tatctccggt ctttcgatta cggacagagc caaactgggc atcgcatata gttttcaaca
      661 gccggcccgt tttaaaggca tttcgttccg tgatttgctg tcgatcgcca cgggaacgga
      721 tgaggagaaa aagtttctgg aaattgtatc ccgggtgggc atttgtccgc tttcttttct
      781 cgataaagaa gtcgattcaa aactttcggg cggcgaaatc aagaaaatcg agctggccac
      841 cacgattgcc accaatccga agctggctat ttatgacgaa ccggatacgg gcattgattt
      901 atggaccatc ggtcccatgg tcagtcttct caaaaaagaa ctgacgccta aaacaacaac
      961 gatcgttgtc agccacaaca aggcttttct ggatgtcgca gacgtgatca tcattttaaa
     1021 tcagggacgc atcgcctata ccggcaatct gaaagatgcg ctgccgttgc tgaatgacat
     1081 gagtatttgc gcataccaca acaaattaga gggatttgaa aatgctgaat gctttagata
     1141 aaaaactgct gaagaccgtt gccgatctgg aaagcctgcc taaaggcgcc tacaatatca
     1201 ggaaaaacgg taaactgctg catcgcgaag tctcggccaa tattaatatt gaaaccaacg
     1261 ccgacggaac gggtattatt gtgaccatta aaccggggac caaaaacgaa tccgttcata
     1321 tcccggtcat tttgagccag gccggacttt atgatgttgt ttataattca ttcatcatcg
     1381 gcgaaggagc agaggtaacg atcgtggccg gttgcggcat tcactgcggt tcgtccgaag
     1441 ccgaaggcca tgcgggcatc catgattttc atgtaggaaa gaaggcaaaa gtcacctatg
     1501 tggaaaaaca tttcgccatg ggagagggaa aaggaagacg atcgcttaat ccaacaacaa
     1561 aagtctttct ggatgaggga gcaacagcgg aaatggagct gacgcagata agcggtgtgg
     1621 atgaagccaa ccgtcttaat gaagcggaac tcggtccgga cagtctgtta ctgattatgg
     1681 aacgcgtcct gacggaaggc gaacaatcgg ctgtatccac caataacatc atactgaaag
     1741 gcttcggcag cagggccaat atgatttccc gctcggtgat gaaaggcaat tccaagcaaa
     1801 tctttcacgc gaacatggaa gccaggaaca aatgcttcgg acacatcgaa tgcgatgcta
     1861 ttatcatgga caacggcacc aacgaaacca tgcccgcact gaaggccctg catccggacg
     1921 cggaactgac gcatgaggcc tccatcggca aaattgccaa tgaccagttg acgaaactca
     1981 tgagcctggg actgacttac gatcaagcca tcaacagaat tattcagggc ttcctgaaat
     2041 aaaaaaattg atccgtccca acaagagtaa atccgaatga caaaaaacat ccatccccct
     2101 gtcacattgc ataacttact ggttacggga ggctgcggtt ttatcggcag caatttcatc
     2161 cggtatttaa tcgaccggat tcatcattca ggaagaatta tcaatgtcga caaattaact
     2221 tacgcgggga atcctgagaa tcttgcagag attgccgcaa acggtgcatc ccgatacatt
     2281 tttcaaaaag cggatatatg cgacagggcc cgcctggaag aaatcttttc tcaatacgat
     2341 attgacggcg tttgccactt tgcggccgaa tcgcatgtgg atcgttccat caagacgccc
     2401 ggagatttca ttcagaccaa catcgtcgga accttcaatc ttctggagtg cgccaaagcg
     2461 cgcggcgacc ggttcaagat ttttcaccac gtcagtaccg acgaggtcta cggcagtctt
     2521 ggagcggacg gccttttccg tgaagacacg ccttatcgtc cgaacagccc ctattccgca
     2581 tccaaagccg cttccgatca tctggtgcgg gcttactata agacgttcgg attgcccgtg
     2641 accatttcca actgttccaa taattacggt ccctatcagt ttccggaaaa gctcatcccc
     2701 ctgatcgttc tcaacgccct ggaaggcaaa ccgcttcccg tttacggtga cggtaaaaat
     2761 gtccgcgact ggctttatgt cgaggaccac tgtgaggcca tctggaccat catgacgtcc
     2821 ggccggcatg gcgaaacgta caatgtcggc ggcaaggcgg aaatggaaaa tattgccatt
     2881 gtgcagttga tctgcgatat tctcgatgat atacaaccgg cgtccgacaa tagcccgagg
     2941 cgccggttga ttacctttgt gaaggatcgc cccggacacg accgccgtta tgccattgat
     3001 ttttctaagc tgcaaaagga actgaactgg gcgccgaaag aatcgttttc ctccggtctg
     3061 caaaaaaccg ttaactggta cctcgaccaa aagaaatggg ttgaaaacat caaaaccggt
     3121 gcttacctga attggatcaa agagcagtat ggtgcgtagt tttctcgaga aaagaatcac
     3181 cctttgcccc ggggcaagaa ggttctctgt ttataaatta taattccccg cagcctccgg
     3241 ctgataaata acatcattga cctttatttt cctgacgccg gacggcacag gccattcgat
     3301 gatatcccct ttacgatacc ctatgagagc cattcccagc ggaaccagaa tggacagttt
     3361 gttttccgca atattggctt cggatggaaa aaccagggta taggtcattt gttctccgga
     3421 ttcaatgtcc gtaacgctga cccgggaatt catcgtgatc acatccgccg gcacatcctc
     3481 agaatcaacc accgtaccgt tgcacaattc cttttccaac aggtcgatat tggctttgtt
     3541 gtgcctgggc gttgaccgca cgccgtcaat taattccagt aaacgatcca tatcgaattt
     3601 tgtaatgtat atcgtgcgat ctgccatgat gatccatctc cctgaagttc attgcttcgt
     3661 tattatgttt ggataaaaat aaggttaaat gctttttcaa atccgttgta taattactat
     3721 atgcaaataa aagccggctg tcaatccctt aagcatattt c
//
LOCUS       DAOSME010000136         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_26596_length_3755_cov_20.283514, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000136 DAOSME010000000
VERSION     DAOSME010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26596_length_3755_cov_20.283514"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLG98_11325"
     CDS             <1..908
                     /locus_tag="PLG98_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOE33899.1"
                     /translation="GGHLSAFWIVVANSWMQTPAGYIINEAGKIILGNFGDAVFNPST
                     VVRYVHTVIASWITSSVMVAGIAGYYVRKGLHGQTARTMLKIGIILFALTPLLQLGAG
                     HAHAVKVIDLQPEKAAAMEGHFETAKGAKIYALGYVDEKNRMTYGIYIPKGLSFLYNF
                     DWNSEIKGLNDFPKEDWPPVNFVFQVYHIMVGAGVVMIGLGLLGAYLLWKGRLYENQW
                     YLFILPLLIPLPHIAHETGWIAAELGRQPWIIYRLMKTSDAASVVVSAGEITFSLIMF
                     SLIYLLLGVMFIKLAITVVRNGPAA"
     gene            953..1963
                     /gene="cydB"
                     /locus_tag="PLG98_11330"
     CDS             953..1963
                     /gene="cydB"
                     /locus_tag="PLG98_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOE33900.1"
                     /translation="MDNIQCYQELWFVLILVLLMGYSLLDGFDLGIGALLPFLGKTKA
                     EKDILINSIGPVWDGNEVWLITGGGALFAAFPHAYATAFSGFYLAMMLVLFALIFRAV
                     SMEFRAHDEKRSLLWEKAFIAGSALAALLFGVALGNVVYGVPLDDQMEFTGNFFTLLR
                     PVPLMFGVTGFAAVLLQGAAYAVMKTEGELRERSFKAVSFLTVINLITAMIYFGTIAF
                     TFDDIVTNWLFYLAFMCTVLWLSSIFFSVRHKNDSAPFWESSFAFIGLWLVVAAVHFP
                     NLIRASNDQSMSLTIYNSSTSLLTLKVMSVIALIGMPIVIAYTIFVYRIFKGKTKSSS
                     HY"
     regulatory      2014..2115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2290..3093
                     /gene="thiM"
                     /locus_tag="PLG98_11335"
     CDS             2290..3093
                     /gene="thiM"
                     /locus_tag="PLG98_11335"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOE33901.1"
                     /translation="MRMTAKEIYRSIELIRSRAPVIHNITNYVVMNNTANALLAIGAS
                     PVMAHAQEEVEDMVDIASALVINIGTLSESWIRSMFKAAHQARKKGIPVILDPVGAGA
                     TNYRTNTARALMAQEPPSIIRGNASEIMALYDAQSKTKGVDSASSSDQAIDTAQKLSE
                     MHECVVCVSGATDYIICPRTILKIKNGNPMMSRVTGLGCTASALCGAFAAVGESTFAS
                     TAQAMALMGIAGETAAEVAAGPGSLQVHFLDALYRISEDDIARRLKVED"
     gene            3096..3719
                     /gene="thiE"
                     /locus_tag="PLG98_11340"
     CDS             3096..3719
                     /gene="thiE"
                     /locus_tag="PLG98_11340"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOE33902.1"
                     /translation="MRGLYLVTDRRLCKDKSVEEIVLQAVKGGVSYVQLREKEMSTRD
                     FVEEALRIKKSLTPHKVPLIINDRVDVALACGADGVHIGQGDMPYDLARRLLGPKAII
                     GLSVETWADFIASQALDVSYIGVSPVFATPTKTDTKGVWGLEGLSRIKAFSRHPLVAI
                     GGIHEANIREVIEAGAPCIAVVSAICSSPDPETAAGRLARLINAALK"
BASE COUNT          885 a          840 c         1029 g         1001 t
ORIGIN      
        1 tcggcggcca tctttccgcc ttctggatcg tggtggccaa ttcctggatg cagacaccgg
       61 ctggttatat cattaatgaa gcgggtaaaa tcattttagg gaattttggc gatgcggttt
      121 ttaatccgtc aacagtcgtc cggtatgtcc atacggtcat cgcttcatgg atcaccagtt
      181 cggtgatggt ggcgggtatt gccgggtatt atgtgagaaa aggtctccac ggtcagacgg
      241 ccagaaccat gcttaaaatc gggatcatcc tttttgctct gacaccgctt ctgcaattgg
      301 gggcggggca tgcccacgcg gtgaaggtca tcgatctgca gccggaaaaa gcagccgcga
      361 tggaagggca ttttgaaaca gcgaaaggcg ccaagatata cgccctgggt tatgttgatg
      421 agaagaaccg gatgacgtat ggcatctaca ttcccaaggg gctgagtttt ctttacaatt
      481 ttgactggaa ttcggaaata aaaggcctca atgactttcc gaaagaagac tggccgccgg
      541 taaactttgt gtttcaggtg tatcatatca tggtgggtgc cggagtcgtc atgatcggcc
      601 tgggattgct gggggcgtat ctgttatgga agggccgact ttatgagaat caatggtatc
      661 tttttatttt gcccctcctg ataccgctgc cgcatattgc ccatgagacc ggctggatcg
      721 cagcggaatt gggacgccag ccgtggatta tctacaggct catgaagacc tcggatgctg
      781 catcggtggt tgtttccgcg ggagaaataa cgttcagcct gatcatgttt tctcttattt
      841 atctcctctt gggagtgatg tttatcaaac tggccatcac cgtcgttcgg aatggaccgg
      901 ccgcttaatg aaaaaattct aaaaactgga aattattctg gaggcaacat ccatggacaa
      961 tattcaatgc tatcaggagc tctggtttgt tctgatctta gtgcttctca tggggtattc
     1021 tcttctggac ggatttgatc tgggcatcgg agcgctgctt ccttttttgg gcaagaccaa
     1081 agcagagaag gatatcttaa tcaattccat cggtccggtt tgggacggca atgaagtctg
     1141 gctgattacc ggcggcggcg ctttatttgc cgctttcccg catgcctatg ccacggcttt
     1201 ttccggtttt tatctggcga tgatgctggt gttgttcgct ctgattttcc gggctgtttc
     1261 catggaattt cgtgcacatg acgaaaaaag atccctcctt tgggaaaagg cgttcattgc
     1321 cggcagcgcg ctggcggctt tgttgttcgg cgtcgcactg ggcaacgtcg tttacggggt
     1381 gccgctggat gatcaaatgg aatttacagg aaacttcttt acactactgc gtccggtgcc
     1441 gctgatgttc ggggtaaccg ggtttgccgc tgttttgttg cagggagcag catacgcagt
     1501 gatgaaaacg gaaggtgagt tgcgagaacg gtcctttaaa gcggtgagtt ttctaacggt
     1561 aataaatctc ataacagcca tgatctattt tggaacgatt gcctttacct tcgatgacat
     1621 cgttacaaat tggttattct atctggcctt tatgtgcacc gtgctttggc tgtcatcgat
     1681 tttcttttcc gtcaggcata aaaacgactc tgctccgttt tgggaatcgt cattcgcctt
     1741 tatcggcctg tggctggtcg tggccgccgt tcattttccc aatctgatta gggccagcaa
     1801 tgatcaaagt atgagcttga caatttataa cagttccacc agtttgttaa ccctcaaagt
     1861 catgagcgtt attgctctga tcgggatgcc gattgtaatt gcctatacca tttttgttta
     1921 tcgtatcttc aaaggcaaaa cgaaatcttc aagtcattat tgaaaggcag ccatagtgaa
     1981 tgtgattgac gccaatcaat cacattggtc agatattttg cataggggtg gtccggaaga
     2041 taacggatcc gagaagatac ccgttgaccc tgatctggat aatgccagcg aagggagaat
     2101 gtttgttgca tgtaaaccct tcccggtatg gcccgtccgg aagggtttta aatgagggac
     2161 aaactccgga aacggagtgc accgaagact gctgatcgaa tgatcccctc ggggcgactt
     2221 gtttttgcgg ggaagtatcc ctttaaatat tgctttgggt tttgcaaacc atcaaggagt
     2281 tggaagatca tgagaatgac ggcgaaagaa atatacagat cgatagagct gattcgttcc
     2341 agagcgccgg tgatccataa cataaccaat tatgtggtca tgaacaatac ggccaacgcg
     2401 cttttggcaa tcggcgcatc cccggtcatg gcgcatgcgc aagaggaagt tgaagacatg
     2461 gtggacatcg cttcggctct ggtgatcaac atcggaacct taagtgaaag ctggatccgc
     2521 tcgatgttca aagccgcgca tcaggcccga aaaaaaggca ttcccgtaat tctggatccg
     2581 gttggcgctg gagcgaccaa ctaccgtacg aatacggcgc gagcattaat ggcgcaagaa
     2641 ccgccttcga tcatcagggg taatgcgtcg gaaatcatgg ctctttacga tgcgcaatcc
     2701 aaaacaaaag gggtggacag cgcctcgtct tccgatcagg ccatagatac agcgcaaaaa
     2761 ttaagcgaaa tgcatgagtg tgtcgtgtgc gtgagcggag caacagatta cattatctgt
     2821 ccgcgaacaa tattaaaaat caaaaatggt aacccgatga tgtccagggt cacaggattg
     2881 ggctgtacgg catcggcgtt atgcggagcc tttgccgcgg tgggagaatc gacttttgct
     2941 tccaccgctc aggcgatggc gctcatgggg attgccggag aaacggcggc agaagttgcc
     3001 gccggaccgg gaagtcttca ggttcatttt ttggatgctc tttaccgtat atcggaagat
     3061 gatatcgcga gacgtttaaa ggttgaggat tagacatgcg gggattgtat ctggttaccg
     3121 accgccgttt gtgcaaagac aaatcggtag aggaaatcgt tcttcaggcg gtcaaaggcg
     3181 gagtgtcata tgttcagctt cgcgaaaaag aaatgtcgac ccgtgatttt gttgaagaag
     3241 ctctgcgtat taagaagagt ttaacgcccc ataaagttcc cctgatcatc aatgaccgtg
     3301 tggatgttgc tttggcctgc ggcgccgacg gcgttcatat cggacagggg gatatgcctt
     3361 atgacctggc gcgcagactg ctgggaccca aagcgatcat cggtctttcc gtagaaacgt
     3421 gggcggattt tattgccagt caggcgcttg atgtcagtta tattggcgtc agtccggttt
     3481 ttgccacacc gaccaaaacg gatacgaaag gtgtatgggg tttggaaggt ctttcccgca
     3541 tcaaggcgtt cagtcgtcat ccgctggtgg cgatcggcgg tattcatgaa gccaatatca
     3601 gggaggtcat tgaagccgga gcaccgtgca tcgcggttgt ttctgcgata tgttcctcgc
     3661 ccgatccgga gaccgctgcc ggtcggttgg cgcgcttaat caatgccgcc ttgaaataaa
     3721 ttgagttatc caaagacgga tgcgcctccg acgga
//
LOCUS       DAOSME010000137         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_27331_length_3675_cov_31.830663, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000137 DAOSME010000000
VERSION     DAOSME010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27331_length_3675_cov_31.830663"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="PLG98_11345"
     CDS             complement(<1..1699)
                     /locus_tag="PLG98_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33903.1"
                     /translation="MMHTPEKILEILPLLDEQIADDLENETLDFKECQTEAKLREFAK
                     EMAVCLGNASGGTVVFGVKDRVLGCDKAIVGLDFAPDLGALGATLYDSIDPKLPVQFE
                     WLLFGNRRLLLMHVHPSMPPYTTTSGKGWMRVGKECKPLTGSLLRQLREKAGLSDPTA
                     NVLPLDSPFRAISPAAIEILRREMKGMGSPGDLLSCDDMDLCGKLGFLKNGRLTMAGL
                     LIAGRDELLAEHVPNHEWKYSRMRTDTDYESPPVGGRDCILVALDKMMLLLGQQNPIT
                     TIPSGLFHAEFPQYPTIALREALMNAFAHRDYNIPGMVFIRHFHDRFEINSPGGFVGG
                     VTPENILHHPPVTRNRYLVETVLLATRLVNRQNLGVPRIFRALLEEGKEPPAFDEIGQ
                     AVRVTFPGQKIDQAFKALLSFLTDQQGAYLDVDDLLLLHFFRRRHEARWNEVREVYPY
                     DERRLREKLAYLETHLLMLEHSGTGRGTVYRLSRRASAILNEGVTYDMTRRLDKEAIK
                     VRILSLLKDRSLRNADIRNFTDLGRQQAYRIMKELESDDLVYQEGHGQAALWHLKQSL
                     "
     gene            complement(1969..2418)
                     /locus_tag="PLG98_11350"
     CDS             complement(1969..2418)
                     /locus_tag="PLG98_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33904.1"
                     /translation="MSRREKHVGKSQNAREFYSRYLKNIDCEPTVDDLIALAPSTEGG
                     EELREPTSKRKRTVSASQKLQEHFQEHWVEWLFGSIVAITAWSVIYARIDLARIETNQ
                     ITQKECVTVLQSGTQRASDKNHEQDLILREHALRISNVEEKVNKPKK"
     gene            complement(2419..3129)
                     /locus_tag="PLG98_11355"
     CDS             complement(2419..3129)
                     /locus_tag="PLG98_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33905.1"
                     /translation="MIDKVDENLVGMVMGIRFRANFSIEDQVGQMVDHLLYSKKAFFD
                     PKVFPVVRSGVGKKMLLNEATNDSLRIDNSNIILEITFGGTFTAADTTKLQQQFNSEV
                     ITGIMRVFKIKEIVRLGYVQRYVFKMEELAKKFVDKTIGQTLGGINDINLSFSKRLPV
                     NEALVKQYVNDYDNAIFNVIKKADLNEIFMSIDYQRYYEPFLESSSQIQFDEFISQAD
                     GFTQKKYLAWLNSNYIED"
     gene            complement(3472..>3675)
                     /locus_tag="PLG98_11360"
     CDS             complement(3472..>3675)
                     /locus_tag="PLG98_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33906.1"
                     /translation="YKTAEEDDPRPGLDYLKQEVANYWDRRQMIIALLNFLSHKPSSA
                     MPHWQKDVEAAHLLLGAVEGDGV"
BASE COUNT          923 a          925 c          829 g          998 t
ORIGIN      
        1 tcaatgattg cttcaaatgc catagggcag cttgcccatg cccttcctga tagactagat
       61 cgtcgctttc cagttccttc ataatccgat aggcctgctg acgccccaaa tccgtaaagt
      121 tccgaatatc ggcgttgcga agtgaacgat ctttgagcaa tgacaggatc cggaccttga
      181 tcgcctcttt gtcaagcctt cgcgtcatgt catacgtaac accctcgttc aaaatagcgg
      241 acgcccggcg ggatagacga tacactgtgc cgcgacctgt accgctatgt tccagcatga
      301 ggagatgggt ctccagatag gccagcttct cccgtaaccg ccgttcatcg taaggataaa
      361 cctctcgaac ttcgttccat cgcgcttcgt gacgcctcct gaaaaagtgg aggaggagca
      421 agtcatccac atccagataa gcgccttgct gatcggtgag aaatgacaac agggctttga
      481 aagcttgatc gattttctgg cctggaaagg ttacccgtac cgcctgaccg atttcatcaa
      541 aagcaggggg ttcttttccc tcttccagga gtgcgcggaa gatacgagga actcccaaat
      601 tctgccgatt caccaaacgg gtggccaaaa gcacggtctc gaccagatat cgatttctcg
      661 tgaccggggg atggtgcaga atattttccg gcgtaacgcc gcccacaaag ccgcccgggc
      721 tgttgatctc aaaacgatcg tgaaagtgac ggatgaatac cattcccgga atattgtaat
      781 cccgatgcgc gaaagcattc atcaaggctt cccggagagc aatggtcgga tattgaggaa
      841 attcggcatg gaaaagtccg gaagggattg ttgtaatggg attttgttgc cccaggagaa
      901 gcatcatttt atccaatgcc accagaatac agtctcttcc gccaacagga ggcgattcat
      961 aatccgtatc cgtgcgcatc cgactgtact tccattcatg attcggaaca tgttcggcaa
     1021 gcaattcgtc tcttccagcg atcagcaaac ccgccatcgt caagcgaccg tttttcagga
     1081 atcccaattt gccgcacaga tccatatcgt cacaggatag cagatcgcct ggagatccca
     1141 tgcctttcat ttcgcggcgg aggatttcga ttgccgccgg cgaaattgcc cggaagggac
     1201 tgtccagggg caggacattt gccgtagggt cgctcaaacc agccttttcg cgtaattgac
     1261 gcaacaggga accggtcagc ggtttgcatt cttttccaac cctcatccac cccttgccgg
     1321 aagtggtcgt atagggaggc atggaaggat gaacatgcat cagcaaaagt cggcgatttc
     1381 cgaacaacaa ccattcgaat tgcaccggca gtttcgggtc aatgctgtcg taaagggttg
     1441 cacccaacgc ccccaaatca ggcgcgaagt cgagccccac aatcgccttg tcacaaccga
     1501 gcacccggtc cttgacacca aaaacaaccg ttcctccaga ggcgttgccc agacagacgg
     1561 ccatttcctt ggcaaattcc cgtaattttg cttccgtctg acattccttg aaatcaagag
     1621 tctcattttc aagatcatca gcgatttgtt cgtcaagcag aggcaaaatt tccagtattt
     1681 tttcaggcgt atgcatcata tatacccttg gttttcgagg aaaagagttt ttgcagcatg
     1741 gtcatctcaa ttgatgttca aattacatca atatttgctg cggcatttaa tccaagttct
     1801 ccctagtacc tgatgatgcc gaccgcctct tagatgccga tacagcattt ccctttttga
     1861 cgagatccaa tatggttatc tttgccgtca tgggcttttc ttccaaatga attaggcagt
     1921 ggccagccaa aaaccagcaa tggtaagggg cgatgcgttg ttgggggctt atttcttagg
     1981 tttgtttacc ttctcttcta cattggatat tcggagtgcg tgctcgcgaa gtatcagatc
     2041 ctgttcatga ttcttgtcgg aagccctctg tgtcccactt tgaaggacag ttacacactc
     2101 cttctgcgtt atttggtttg tctcaattcg cgcaaggtcg attcttgcat agataactga
     2161 ccatgcagta atcgcaacaa ttgaaccaaa taaccattcg acccagtgtt cctgaaagtg
     2221 ttcctgcaat ttctggcttg cactgaccgt tctctttcgc ttcgatgttg gttcacgaag
     2281 ctcctccccg ccctccgtag aaggcgcaag ggctataagg tcgtcaacag ttggttcgca
     2341 gtcgatattt ttcaggtatc gcgagtaaaa ttccctcgca ttctgtgatt tgcctacgtg
     2401 tttttctcgt ctgctcattc agtcctcaat gtagttgcta ttcagccagg caaggtattt
     2461 cttttgggtg aagccgtcag cctgtgaaat aaattcgtca aattgtatct gtgatgatga
     2521 ctcaagaaac ggctcatagt atcgttgata gtcaatcgac atgaatatct cattgaggtc
     2581 agctttcttg atcacattga agatggcgtt gtcgtagtca ttcacatact gcttaacaag
     2641 tgcctcattg accggtaatc gtttggaaaa actaaggtta atgtcattta tacctcctaa
     2701 tgtctgtcca atagtcttgt cgacaaactt tttggccagt tcttccattt tgaagacata
     2761 gcgctgtaca tacccaagac ggacaatctc tttgatctta aagacgcgca ttatgccggt
     2821 gattacctcg gaattgaact gttgctgtag cttggtcgta tcggcggcag tgaacgtgcc
     2881 gccaaaagtt atctcaagta tgatgttaga gttgtctata cgcagagaat cattcgtcgc
     2941 ttcattcaat aacatcttct taccaacacc actcctgact acaggaaaca ctttgggatc
     3001 aaaaaaggct ttttttgaat acaagagatg gtccaccatt tggccgactt ggtcctcaat
     3061 tgagaagttc gcacgaaaac ggatccccat caccatacct acgagatttt cgtcaacttt
     3121 gtcgatcatt taattgcctc cttacatatc ccccaaaaca gaactaaact gtgccattaa
     3181 ctcgtctggt tagaatgtgg acctttcatg tgagttgcca acttcatcaa atgaaacagc
     3241 ccattctttt gtaagaaacg ggggggcagc ccctttgtcc atatttacct aaaacgcggt
     3301 cataagggat ggaacttatt aaacgacatt taatgatgtt ttttcttatc acgattcgaa
     3361 ctcacaatct actatggaat agattattcg gccagtaagt ttttcagaaa acaaacgcgc
     3421 ctttacgtca taaatcctct aaaaatctcg gtggaaggag tagattataa atcatacccc
     3481 atccccctcc acagcgccca agagcagatg ggcggcttcg acatcttttt gccagtgggg
     3541 catggccgat gagggcttgt gggagagaaa gttcagcagg gcaatgatca tttggcgccg
     3601 gtcccaatag ttggcaacct cctgcttcaa ataatccagg ccggggcggg gatcgtcctc
     3661 ctcggcggtc ttgta
//
LOCUS       DAOSME010000138         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_27454_length_3662_cov_23.540061, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000138 DAOSME010000000
VERSION     DAOSME010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27454_length_3662_cov_23.540061"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLG98_11365"
     CDS             complement(<1..572)
                     /locus_tag="PLG98_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOE33907.1"
                     /translation="MFQLNKKHSIQYYESSFLSGCDFLTHAFCTRLGGLSEDDYKSLN
                     ISFKEGDLESRVLQNWNRLAMAFAIPLENFLTLNQVHGDDIFIIKPYGDYLPSDGALN
                     YDAIVTPRENLAICIKTADCVPVFIVDRAKKIIAAVHAGWKGTALEISAKVVRLFLDK
                     YGSYPHDILAAVGPSIGLCCFEVDEDAADS"
     gene            complement(576..1589)
                     /locus_tag="PLG98_11370"
     CDS             complement(576..1589)
                     /locus_tag="PLG98_11370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE33908.1"
                     /translation="MVGQAEKIKFTVDKSQGGQRLDIYLAQSEAALSRSQVKYAIEEG
                     DVLVNDKVPKVSQHLKEGDVIVLTKRPPVEAIAVPQEMPLDIVYEDDAVIVINKPAGM
                     VVHPAPGNPDKTLVNALLFHCHDLSGIGGVLRPGIVHRLDKDTSGLIVAAKSDDAHRN
                     LSSQFEKHDVHKKYLALVWGDVKGNSGEIVLPVGRHTNNRKKMSTKSRHGKGALTLWK
                     VRERFGVATLLDVEIKTGRTHQIRVHLSDRGYPVVGDAVYGNSAKINTVKDPLLKAKI
                     KEFNRQALHAAQLSFIHPQKGERVVFTADMPQDLADLCAQLRTSIKSVDSKKDMWKDK
                     LKG"
     gene            complement(1727..2320)
                     /locus_tag="PLG98_11375"
     CDS             complement(1727..2320)
                     /locus_tag="PLG98_11375"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOE33909.1"
                     /translation="MATVKRIKSDNVFQLKITLNHIKPPIWRRVLVDSDIKLPDLHKI
                     IQTVMGWTNSHLHQFIIGNQYYSLPSDESFYKVVDYRRIKLDSLFNTPKSNFIYEYDF
                     GDGWEHSIVIEKILPRKKNTYYPICIDGKRSCPPEDCGGTFGYENLITIINNPEHEEY
                     DEMMEWLGDYFDPKEFNIDEVNEFLREKNFGCVELFD"
     gene            complement(2492..3622)
                     /locus_tag="PLG98_11380"
     CDS             complement(2492..3622)
                     /locus_tag="PLG98_11380"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE33910.1"
                     /translation="MYIAQKQLEKLRNLAVPGKVVVIYGARRTGKTTLLKEFLKDETK
                     DHLLVNGEDITVHSYLSSQSVEKLTSFVGNAKLLIVDEAQKIPNIGINLKLIVDHLSG
                     IRMVVTGSSSFDLARHTGEPLTGRKYTLKLFPLAQMEIGQIERIHQTQAHLENRLIYG
                     SYPEVVLMQDNQGREKYLKEIVSSYLYKDILELDGIRQSVKIIRLLQLLAFQIGKEVS
                     LTELGTSLSMSKNTVERYLDLLEKAFVLHRLTGFSRNLRSEIIKNCRYYFLDNGVRNA
                     LINNFNPLSQRNDCGELWENYLIMERLKRQEYLDTPANNYFWRTYTRQEIDFVEERQG
                     RLFGYEMKWGKAKPKPPKLWQSAYPESSYELINRENYLKFIT"
BASE COUNT          914 a          864 c          799 g         1085 t
ORIGIN      
        1 aaagaatccg ccgcgtcttc atcaacttca aagcagcaga gtccgatcga ggggccaacg
       61 gccgccagaa tatcgtgcgg atatgatccg tatttgtcca ggaaaagacg aacgactttg
      121 gcgctgattt ccagcgcggt gcctttccat ccggcatgga ctgccgcaat gatttttttt
      181 gcccggtcaa cgatgaacac cggcacgcaa tccgcggttt tgatgcagat cgccaggttc
      241 tcacggggcg ttacaatagc gtcgtaattc agggctccgt ccgaaggtaa ataatcgccg
      301 tagggtttga ttataaaaat atcgtcgccg tgcacctgat tcaaggtcag gaagttttcc
      361 aggggtatgg caaaggccat ggccagacga ttccagttct gcaaaaccct gctttccaga
      421 tcgccttcct tgaaactgat gttcagcgat ttataatcgt cttccgagag accgccgagc
      481 ctggtgcaga aggcatgcgt caaaaaatcg catccgctca aaaacgagga ttcgtaatac
      541 tggatggaat gctttttgtt cagttgaaac atggattacc cttttaattt atccttccac
      601 atatcttttt ttgagtcaac tgacttgatg cttgtccgta attgagcgca caaatccgcc
      661 agatcctgcg gcatgtccgc ggtaaatacc acccgctcgc ctttttgcgg atgaatgaaa
      721 gaaagttgtg ccgcgtgcag tgcctgccgg ttaaattcct tgatctttgc cttcagcagg
      781 ggatctttaa ccgtgttgat tttggcggag ttgccgtaaa cggcatcgcc caccaccgga
      841 taaccgcggt cggacaaatg cacgcggatc tgatgcgtgc gtccggtttt aatctccaca
      901 tccagaagcg ttgccacgcc gaagcgttcg cgcactttcc aaagagtgag cgcgcctttg
      961 ccgtgtctgc tttttgtgga catttttttg cggttgtttg tgtgcctgcc gacaggcaga
     1021 actatttctc cactattgcc tttcacgtct ccccagacga gcgccagata ttttttgtgc
     1081 acatcgtgtt tttcaaactg cgaagaaaga ttgcggtgcg cgtcatcgga ttttgccgcc
     1141 acaataaggc cggacgtgtc cttatccagc cggtgcacaa tgcccggacg caagacaccg
     1201 ccgatgccgg ataaatcatg gcagtggaaa agcaaagcat tgacgagcgt tttatcggga
     1261 ttcccgggcg ccggatgcac caccataccc gccggtttgt tgatgacgat aacggcgtcg
     1321 tcttcgtaaa cgatgtccag cggcatttcc tgcgggacgg ctatggcctc caccggcggc
     1381 cttttggtca gcacaatcac gtcgccttcc ttgaggtgct ggctgacttt gggaacttta
     1441 tcgttgacca gcacatcgcc ttcctcaatg gcgtacttca cctgcgaacg cgaaagcgcc
     1501 gcctcgctct gcgcgagata tatatccagc ctctgcccgc cctggctttt atcaactgta
     1561 aattttattt tttccgcctg tccgaccatc ttattgcctc aattttgtca ttcccgctaa
     1621 ggcgggaatc cagtcttttt cattttgaaa aacttgataa ctcaaaggcg ttccatgttt
     1681 ccataaaaat agaaacgaat atattataaa ttatcaatac cttcttttag tcaaataatt
     1741 cgacacaacc aaaatttttt tcacggagga attcattcac ttcatctata ttgaactcct
     1801 tcgggtcaaa ataatctcca agccattcca tcatttcatc atattcttca tgttcgggat
     1861 tgtttattat cgtaataaga ttttcataac caaaggtgcc accacaatct tcaggtgggc
     1921 aacttcgttt gccgtcaatg catattggat aataagtgtt ttttttacga ggcagtattt
     1981 tttcaataac gattgaatgt tcccaaccat ccccaaaatc atattcataa ataaaatttg
     2041 attttggcgt attgaataat gaatccaatt taattcggcg ataatcaact actttgtaaa
     2101 atgattcatc gcttggaagt gagtaatatt gattgccaat aataaattga tgtaaatgag
     2161 aattagtcca gcccattaca gtctgtataa ttttatgtaa atcaggcagc ttaatatctg
     2221 aatcaaccaa tactcttctc caaatcggtg gcttaatatg attaagagtt atttttaatt
     2281 gaaatacatt atcagattta attcgtttaa cagttgccat gtcaacacct tgttctttta
     2341 gattaaatat tgaaatgttt gtcccactga aaaatcagcc tctgcccgct ctggctttta
     2401 tccactataa aactgatttt ttcagacgtc cctaacaagt atttgctccg gtattttagt
     2461 tatggaactg ttcgggaaag cagttcctgc atcaagtaat aaacttgaga taattctctc
     2521 tgttaatcag ctcataggaa gattccggat aggccgactg ccagagtttc ggtggcttgg
     2581 gttttgcctt gccccatttc atttcatagc cgaacaggcg gccttgacgc tcttcgacaa
     2641 aatcaatttc ctgtctggta taagtccgcc agaaataatt attagccggg gtgtccagat
     2701 attcctgcct tttcaatctt tccatgatca ggtaattctc ccacaattcg ccgcaatcgt
     2761 tgcgttgcga gagcggattg aaattgttga tcagcgcgtt gcgcacgccg ttgtccagaa
     2821 aatagtagcg gcagtttttt atgatttcac tgcgcaggtt gcggctgaaa ccggtcaagc
     2881 gatgcaggac gaatgctttt tccaggaggt ccagatagcg ctccacggta ttcttgctca
     2941 tggataatga tgttcccaat tcggtcagtg atacttcctt gcctatctgg aaagcaagaa
     3001 gctggagcag gcggataatc ttgacggatt gtctgatgcc gtcgagctcc agaatatctt
     3061 tatacaaata agaagacacg atttctttga gatacttttc ccgcccttga ttatcctgca
     3121 ttaagacgac ttcgggataa gaaccgtaaa tcaggcggtt ttccagatgg gcttgagttt
     3181 gatgtattct ttcaatctga ccgatttcca tttgcgccag cggaaacaat ttgagcgtgt
     3241 attttcgtcc ggtcagcggt tcgcccgtgt gacgcgccag atcgaaggac gacgagccgg
     3301 tgacgaccat gcggataccg gatagatgat caacaatcag cttgagattt attccgatat
     3361 tgggaatttt ctgcgcttcg tcaacaatca gaagttttgc gttgccgaca aaagatgtga
     3421 gcttttccac cgactggctg gataaatagg aatggacggt aatatcttct ccgttgacca
     3481 gcagatgatc ttttgtttcg tctttcagaa attctttcag cagtgtcgtt ttacccgtgc
     3541 gcctggcgcc gtaaataaca accaccttgc ccggaacggc cagatttctt aacttttcca
     3601 attgtttttg tgcaatgtac atataattac cttggtcatt gtgttgaccg tattatatat
     3661 aa
//
LOCUS       DAOSME010000139         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_27496_length_3657_cov_19.737646, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000139 DAOSME010000000
VERSION     DAOSME010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27496_length_3657_cov_19.737646"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLG98_11385"
     CDS             complement(<1..583)
                     /locus_tag="PLG98_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOE33911.1"
                     /translation="MEMGILGLPQSGKSTLFEIMTGVKSNQSHNETVLRGQASVPDER
                     FDKLVEIYKPAKISPAKVPFVDVHAVGEHPWEAIRQNLSGTDGILHVVDGFSLPDIQL
                     SIEAYHKLEDELVLSDLLIIEKKMEKLAKMPRKAIPAQEAAQNELLPRLKDCLEAGKP
                     LRAAGLTPTEIFTLKSFSFWTIKPELVVVNTAED"
     gene            complement(749..>3657)
                     /locus_tag="PLG98_11390"
     CDS             complement(749..>3657)
                     /locus_tag="PLG98_11390"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOE33912.1"
                     /translation="AIGLLIFLVVLGIAGFFIAPPLMKPYIMKKMSEALHRKASIEKI
                     SINPYAVSITLKKFSLEDPGKPKPFVAFDELYVNADLMSSLFRRALILKQITLTNPYV
                     GISRKPEGSYNFSDLLPKDEAKPDKEDKPFLFSLNNIQIINGNIDFEDAPKKTNHTVR
                     ELNVSVPFISNIEYHLEKYVEPKFSTNINNNKFELAGKTQPFLTSRATSFDINIQDID
                     IPYYLNYVPAKMNCQLKSARLDTNIKLNFIVNEDKSPALTLNGNVVLRDVILDDLKNN
                     KILHLPSLNVNMTSVEPFVSNIHLARIAIESPELVIRRDKEGEINLLNLTKKQVEKLP
                     QKEAAAASKKKSGFKFRVDDVLLYKADIIYMDSQPSDPVKLHFSPLSFKAANLSTEKG
                     ATGKVELEGILNKKGEFKDRVTLTLEPLSVDVQSDWKNIGIRAFQPYFTDKVKMDITR
                     GTISSTGNFSLSRNKKDELVIKCTGSVSVSNLAALDKAQSNDFLKFKRLSLDKIKFGY
                     NPFSLSINTISLADFYTRIIINPDASLNVQDIFGDNQASKETAIKKEEKKPVTAETKK
                     KDNQETADIKIGKVVFKNGHIDFSDNNIQPNYSANMFNLTGSVTGLSSKEFSRADVAL
                     KGNLGYGSPIEIAGKINPLAKDLFVDLKVGFKDIELSPITPYSNKFLGYPITKGKLTF
                     NVSYLIDKRKLDSQNKVLIDQLTFGEKVESPQAVKAPVTLAATLLTDRNGQINLDLPV
                     SGSLDDPKFKVWSVIWQIIVNLITKAVTSPFALLSSLTGGGEELSFIEFDYGSAVISA
                     EETKKISMIGKALNDRPNIKLDIESYVDAEQDKYALKKAELDRRIKAQKMKETMSKDD
                     QQIAVENVVLTEQEYDKYLKQVYRSADFSKPRNILGMQKTLPPAEMEKLMLASIEVTD
                     SDLRQLAARRAQNVKELMLQSDIAADRIFIVESKSLTPEKKEKVKNSRADFKLK"
BASE COUNT          901 a          816 c          838 g         1102 t
ORIGIN      
        1 tgtcctccgc tgtattgacg acaaccaact ccggcttgat cgtccagaag gaaaagctct
       61 tcagtgtaaa aatttcagta ggcgttaaac ccgccgcacg caacggtttt ccggcttcta
      121 gacaatcttt taagcgcggc aataactcgt tttgagcggc ttcctgtgcg ggaatggctt
      181 tccggggcat ttttgcaagc ttctccatct ttttttcaat gattaataag tcggaaagta
      241 ccaattcatc ttccagtttg tgataggctt cgatactcaa ctgaatatcg ggcagggaaa
      301 aaccgtcaac gacatgcaga atcccgtcgg tgccgcttaa attctgacgg atggcctccc
      361 acggatgttc gccaacggcg tgtacgtcga caaaaggcac tttggccgga gaaatttttg
      421 ccggtttgta tatttcaaca agtttatcaa aacgttcgtc aggcacggaa gcttggccgc
      481 gcaagacggt ttcgttgtgc gactggttgc ttttcacgcc agtcataatt tcgaataagg
      541 tactttttcc gctttgggga agacccagta tgcccatttc cataacaaat ctcttttcta
      601 cttaaaaaaa ttatttagaa taaccaaact aatgttcttg taagaaagtc aaaaaatcta
      661 taacatcgtc attccggcga aggccggaat ccagtattga taagctgatt cggaccccgg
      721 cttccgccgg gaatacgaca tttggtgttt atttcagttt aaaatcagcc ctgctattct
      781 tcactttttc ttttttctcc ggtgtcagac ttttcgactc cacaataaat atccggtccg
      841 cggcgatatc cgactgaagc atcagctctt ttacgttttg cgcccgccgg gctgccagtt
      901 gacgaagatc gctgtccgtt acttcaatac tggccagcat taatttttcc atctccgcgg
      961 gcggcaacgt tttttgcatg cccagaatat tgcgcggttt ggaaaaatcc gccgatcggt
     1021 aaacctgttt caggtactta tcatattcct gttctgtaag caccacgttt tcaacggcaa
     1081 tctgttgatc atcttttgac atcgtttctt tcattttctg cgctttgata cggcggtcaa
     1141 gctccgcttt cttcaaagca tatttgtcct gttccgcgtc cacgtagctt tcgatatcca
     1201 gtttgatgtt ggggcgatca ttcagggctt tgccgatcat gctgatcttt tttgtttctt
     1261 ccgcgctgat cacggcgctt ccgtagtcaa attcgataaa actcaattcc tcaccgccgc
     1321 cggtaagaga ggaaagcagg gcaaaggggg aggtgaccgc cttggtaatc agattgacga
     1381 taatctgcca gataaccgac cagaccttga atttcggatc gtccagactt cccgataccg
     1441 gaagatccag gttaatctgt ccgttacggt cggttagcag cgttgccgcc agcgtcaccg
     1501 gcgctttgac agcttgcgga ctttccactt tttcgccgaa ggtcaactgg tcaataagca
     1561 ctttattttg ggagtccaat tttcttttgt cgatcaaata tgaaacgtta aacgtcagtt
     1621 tgcccttggt gatgggatat ccgaggaatt tgttggaata gggggtgatg gggcttaact
     1681 cgatgtcctt gaaaccgact tttaaatcga cgaagagatc cttggccagc ggattaattt
     1741 tccccgcgat ttcaatcggc gagccgtatc ccagattccc cttgagggcc acatcggcgc
     1801 gggaaaattc tttcgaggaa agtccggtaa cgcttccggt cagattgaac atgttcgccg
     1861 aatagttcgg ctgaatgtta ttatcgctga aatcgatgtg accgtttttg aatacgacct
     1921 tgccgatttt aatatcggca gtttcctggt tatctttctt ctttgtttcc gcagttaccg
     1981 gcttcttttc ttcttttttg atcgctgttt cttttgacgc ttgattatca ccgaatatat
     2041 cctgaacatt caatgacgca tcgggattga tgataatccg cgtataaaaa tcagcaagcg
     2101 atatggtgtt gatgctcaga gaaaaaggat tgtagccgaa tttgatttta tccagggaga
     2161 gccttttgaa tttcagaaaa tcattggatt gcgccttgtc cagcgcggcc aggttggaga
     2221 cggaaacaga ccctgtgcat ttaatgacca gttcatcttt tttgtttctg ctcagggaaa
     2281 aatttcccgt tgatgaaatc gttccgcgcg tgatatccat tttaaccttg tcggtgaaat
     2341 acggttgaaa cgcccggatg ccgatatttt tccaatcaga ctgcacatcc acactcagcg
     2401 gctccaaggt cagcgttacc ctgtctttaa actcaccttt cttgttcaaa atgccttcca
     2461 gctcaacttt tcctgttgct cctttttccg tggaaagatt ggcggctttg aaactcagcg
     2521 gtgaaaaatg aagtttgacc ggatcggacg gttgggaatc catgtaaata atatcggctt
     2581 tatacagcaa cacatcatcc acgcggaatt taaacccgga tttcttttta gatgcagcgg
     2641 cagcttcttt ttgcggcaat ttttctactt gcttctttgt cagattgaga agattgattt
     2701 caccttcttt atcacggcgt atgacgagtt ccggagattc gatggcgata cgcgccagat
     2761 gaatattcga tacaaaaggt tcgacagagg tcatattgac gttcaaagaa ggcaaatgaa
     2821 gaattttatt gtttttcagg tcatcgagaa tcacatctct caaaacgaca ttgccgttaa
     2881 gagtcagtgc cggggattta tcctcattga caataaaatt aagttttata ttggtgtcga
     2941 gccgcgcgga tttgagctga cagttcattt tcgccggcac atagttcaga taatagggga
     3001 tgtcgatatc ctgaatattg atgtcgaaac ttgtggcgcg cgaggtcaga aagggctgtg
     3061 ttttaccggc aagctcaaat ttgttattgt tgatattagt ggaaaacttc ggttccacat
     3121 atttttccag atgatattcg atattggata tgaaggggac ggagacattg agttcccgca
     3181 cggtatgatt ggttttcttc ggcgcgtctt caaaatcaat attgccgtta ataatctgaa
     3241 tgttgttcaa cgagaaaaga aaaggtttgt cttctttgtc cggttttgct tcatccttgg
     3301 gaagcaggtc tgagaaattg taggagcctt ccggtttgcg ggaaattccg acgtaaggat
     3361 tcgtcagcgt tatttgcttc aggatcagag cgcggcggaa aagggaggac atcaaatcgg
     3421 cattgacata aagttcgtca aacgcgacaa aaggtttagg cttgcccgga tcttccagtg
     3481 aaaatttctt taatgttatg gaaacagcgt aaggattgat gctgattttt tcaatggacg
     3541 ctttgcggtg cagcgcctcg gacatcttct tcatgatgta cggcttcatc agcggcggcg
     3601 cgataaaaaa tccggcaatt cccagaacaa ccagaaaaat aagaagacca atcgcga
//
LOCUS       DAOSME010000140         3648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_27584_length_3648_cov_21.980518, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000140 DAOSME010000000
VERSION     DAOSME010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27584_length_3648_cov_21.980518"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLG98_11395"
     CDS             complement(<1..605)
                     /locus_tag="PLG98_11395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33913.1"
                     /translation="MKTDETSVKEEMADILPHRLYYESKIKSVHWLNRKRIEHCLDLA
                     GDMTGKRILDAGTGDGYMLQEIKCRELWGIDISRKRLERAAKKAPYAILKQDDLNQSQ
                     LESNFFDGIICADVLEHMRTPANAIREMIRICKPDGFIVVCLPQEINNILARMILWKP
                     PMINPDHYHLVTGFFLQREFKIRPSRVRNIPDVPGPFCLHRV"
     gene            801..2435
                     /locus_tag="PLG98_11400"
     CDS             801..2435
                     /locus_tag="PLG98_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOE33914.1"
                     /translation="MKKVCLLLFLFFIHITLTACGTKQESVSSKPPEEKPPAYGDILV
                     RGDIGDASNLIPILASDSASHNIAGMIYNGLVKYDKNMNIVGDLAESWDISSNGLVIT
                     FHLRKGIKWHDGKPFTSADVLYTYQATIDPKTPTAYAGDFLKVKKVEAPDDYTFRATY
                     EKPFAPALISWSSAILPRHLLAGKDITQSSLSRHPIGTGPYKFKEWVAGQKIVLVSNP
                     DYFEGQPYIDGRMTRIIPDTATMFLELRAQNIGMMGLTPLQYTRQTENNLFKNNFNKY
                     RYLSFAYTFMGYNLKNPLFADKRVRQAISYAINKDEIISGVLLKLGKPASGPYKPGTW
                     AFNDKVKVYNYNPQKALALLNEAGWKKTGSEGLLKKDGQPFIFEIVTNQGNETRQKCA
                     EIIQRQLKEIGITVKIRVLEWSAFVTDFINKRRFDAVILGWTIPLDPDAYDVWHSSKT
                     APEELNFISYNNPEVDEMLEKGRSTFDQKERKKYYDRFQEILAEDQPYTFLYVPEALV
                     IINKRFRGIEPAPIGLEHNFIKWYVPKDEQKYTLTR"
     gene            complement(2490..3281)
                     /locus_tag="PLG98_11405"
     CDS             complement(2490..3281)
                     /locus_tag="PLG98_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOE33915.1"
                     /translation="MQNNNDQQPRRIKIGLFGFGNTGRMVADEFYRDGLFDLEWVVRR
                     THKDNHKYATRLLGYEADDAPIFSVDEVSPEFFSHRPVDIIVDFSSARAHNSYAPAAD
                     VGIKIISAISKYGGKDMEELKQLSAKTAVLYSPNITLGINFLLVASQILQKVAPHADI
                     EIVEEHFRSKKEQSGTAVKIANILGLEKDKHVNSIRVGGIVGKHEIIFGLPNQTIRLI
                     HESISKRAFGQGAIFVSKWLIDAPIGLYSMENIIIDMFKKNIPVY"
BASE COUNT          996 a          890 c          835 g          927 t
ORIGIN      
        1 acccgatgca ggcaaaacgg cccgggaaca tccggaatat ttctgacgcg gcttggacgg
       61 attttaaatt cacgctgcag aaaaaagccg gtcaccagat gataatgatc cggattgatc
      121 atcggcggtt tccacagaat catgcgggca aggatgttgt tgatttcctg cggcaggcaa
      181 accacgataa atccatccgg cttgcatatc cggatcatct cgcggatcgc gttcgccgga
      241 gtgcgcatgt gctccagcac atcggcgcaa atgattccgt caaaaaaatt gctctctaat
      301 tgcgattggt tgaggtcatc ctgcttcaga atggcataag gggccttttt tgcggcgcgc
      361 tccagacgct tccgcgagat atcaattccc cacaattctc tgcattttat ctcctgaagc
      421 atgtacccgt cgccggtacc ggcatcgagt atgcgtttac ccgtcatgtc tccggccaga
      481 tcgagacagt gttcgattcg tttgcggttt agccagtgga cggatttaat cttggattca
      541 taatacaaac gatgcggaag aatatccgcc atttcctctt tgacgcttgt ttcatccgtt
      601 ttcataaatg ataaggctga tccgatgatg ctgtattgtg tgtcgacttt tcaaggccgg
      661 actaggacta taggaagtct gctttttcat gtcaagggaa acccggtcgg ccgataccgc
      721 gatgattgta ccctgagaat ttaaggaatg catcataaaa tttcgttgca atgacatcat
      781 cactgcggta ataaaatgcc atgaaaaaag tctgtctctt attatttctc tttttcatcc
      841 atatcacgct gacggcttgc ggcacaaagc aggaatccgt aagcagcaag ccgccggaag
      901 aaaaaccgcc cgcttacggg gatattctgg tgcggggcga tatcggagac gccagcaacc
      961 tgattcccat cctggcttcc gattcggctt cccacaatat cgcaggaatg atttacaacg
     1021 gtctggtcaa atatgacaaa aatatgaaca tcgtcggcga tctggccgaa tcgtgggaca
     1081 tttcatcgaa tggtctggtc ataacattcc accttcgcaa aggcattaaa tggcatgatg
     1141 gcaagccttt tacctccgcc gatgttcttt acacgtatca ggccaccatc gatccgaaaa
     1201 ctccgaccgc ttacgccggt gattttttaa aagtcaagaa agtcgaggct cccgatgatt
     1261 acacgttccg ggcaacgtat gagaaaccgt ttgcgccggc actgatcagc tggagcagcg
     1321 ccattctgcc gcgacacttg cttgcgggaa aagacattac acaaagttct ttatcacgac
     1381 atccgatcgg caccggtccg tacaaattta aagaatgggt ggccggtcag aaaattgttc
     1441 ttgtttccaa ccctgattat ttcgaggggc aaccctatat cgacggacgg atgacgcgca
     1501 ttatccccga cacggccacg atgtttctgg agttgcgcgc acaaaatatc ggcatgatgg
     1561 gcctcacgcc tctgcaatac acgcggcaaa ccgagaataa tttatttaaa aataatttta
     1621 acaagtaccg gtatttgagc tttgcctata ccttcatggg ctacaacctg aagaatccgc
     1681 tttttgccga caaaagagtg cgacaggcga tttcctacgc gatcaacaag gatgaaatca
     1741 tcagcggcgt attgctgaag ctggggaaac ccgccagcgg cccgtataaa ccgggtacgt
     1801 gggccttcaa cgacaaggtg aaggtgtaca attacaatcc gcagaaagcc cttgcactgc
     1861 tcaacgaagc gggatggaaa aaaaccggca gcgaaggact cctgaaaaaa gacggacaac
     1921 cgtttatttt cgaaatcgtc accaatcagg gcaatgaaac acggcaaaag tgcgccgaga
     1981 tcatccagcg gcagttgaaa gaaatcggca tcaccgtcaa aatccgcgta ttggaatggt
     2041 ccgcgtttgt aacggatttt atcaacaaac ggcgcttcga tgctgtgatt ctgggatgga
     2101 ccatcccgct tgatccggat gcctacgacg tctggcactc gagcaaaacg gcgccggaag
     2161 aattaaattt catttcctat aacaatccgg aagtcgacga aatgctcgag aagggacgca
     2221 gcaccttcga ccagaaggaa agaaaaaaat actatgaccg ctttcaggaa attctggccg
     2281 aagatcagcc ctatacattc ctgtatgttc ctgaagcgct ggtaattatc aacaaacgtt
     2341 tccgtggaat cgagccggcg ccgatcggac tggaacacaa tttcatcaaa tggtacgttc
     2401 cgaaagacga acaaaaatat accctgaccc gatagccgca acaaatcaat acacggttac
     2461 gcccgcggca tcatttccga aatgaaaatt taataaacag gaatgttttt tttgaacatg
     2521 tcgatgatga tattttccat ggaataaaga ccgatgggtg catcgatcag ccatttggaa
     2581 acaaaaatgg cgccctgtcc gaaagccctt ttgctgatgc tttcatggat gagcctgatg
     2641 gtctgattgg gcaggccaaa aataatctcg tgtttgccca cgatgccccc tacccgaatg
     2701 gagttgacat gtttatcttt ttccagtccc aggatattcg ctattttgac ggcggttccc
     2761 gattgttcct tcttggaccg gaaatgttct tccacaattt caatatctgc atggggagca
     2821 actttctgga ggatttgaga tgccaccagc agaaaattga tgcccagcgt aatattggga
     2881 gaataaagaa ccgctgtttt ggcggaaagt tgttttaatt cttccatatc ctttccgccg
     2941 tatttggaga tggcagaaat aattttaatt ccgacatcgg cagccggcgc ataactgttg
     3001 tgcgctctgg cgctggaaaa atccacgatg atatccaccg gacgatgaga aaagaattcc
     3061 ggcgatactt catccaccga aaagatcggg gcatcgtctg cttcatatcc caaaaggcgg
     3121 gtggcatatt tatgattgtc cttatgggtt cgtctgacca cccattcgag atcaaacaat
     3181 ccatccctat aaaactcatc cgccaccatc cggccggtat tgccgaatcc aaaaagcccg
     3241 atcttgattc ttctgggctg ttgatcattg ttgttttgca tggctaaatc ctttaaattc
     3301 cgcagctgtt tgatcctgca cggttttacc aaccgataaa gctttcataa taaaaaatta
     3361 aatgccctgc catcaggcag atggcgtccg attatcttaa cgcttcattt gtatgttata
     3421 agttttggaa ccgcttttat gtgaaaaaat ttcttcagac cgcatgatat tgcttttttt
     3481 agttaaataa catgcagatc ataaacatgc aaggcttgcg ttgccctcgg acacagcgtt
     3541 tttaaaaaat aaaaaagcgc ggatcactga ccgatccgcg ccgtttttta ataatggtgc
     3601 cgaagactgg atttgaacca gcacgggtga tacccaccac cccctcaa
//
LOCUS       DAOSME010000141         3643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_27625_length_3643_cov_19.707079, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000141 DAOSME010000000
VERSION     DAOSME010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27625_length_3643_cov_19.707079"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..78)
                     /locus_tag="PLG98_11410"
     tRNA            complement(3..78)
                     /locus_tag="PLG98_11410"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Glu,seq:ttc)
     gene            complement(309..1010)
                     /locus_tag="PLG98_11415"
     CDS             complement(309..1010)
                     /locus_tag="PLG98_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HOE33916.1"
                     /translation="MSKRNVCITVVLMLLMTAVTCFSADKYQWKLVDTEDNCQIYTSA
                     VPGKDYIAAKATCLIPARIEVIGTILRDIPNYPEWMHDCKATKMLKTVDDEKDVFIFW
                     FRQHIDLLTDRDMILKSKTVIDMQNGTNLVYADSTNELPYDSGKGYVRMPSFYSLFTL
                     QWVDRERTRVTFMIDPNLGSGVPSGIANSKIKSTPFKTLQNMMKMVKKSKYIEAAKTS
                     KYNKMAEDFVKAGHK"
     gene            1228..1593
                     /locus_tag="PLG98_11420"
     CDS             1228..1593
                     /locus_tag="PLG98_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOE33917.1"
                     /translation="MSEEIHKKSHKEPDEKHLDKMTVKELREIASEIPHEKAVHEMKK
                     EELIAFIKEARGIKDDEPVKKKHIGKVKMTKPELKAKIRECKASRLKALESKETERAV
                     WFRHQISQFKKKLRRIAGA"
     gene            complement(1792..2835)
                     /locus_tag="PLG98_11425"
     CDS             complement(1792..2835)
                     /locus_tag="PLG98_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOE33918.1"
                     /translation="MTNVNDFIGKYRNIITIALSLIGIVLMAYYDYCDTECSYLRGDL
                     LGIDLKWVGIAYMAIIIIFAAFKQTPFVRALLAAGLGVEVHLYAFQIQNNVYCPFCLA
                     FSVMLILSFIINYEVPSAWREKRGRMWLYFLGEVDFPMFKIHKLPLLIFSLLGYLTVF
                     LTFNGSVTPAYGQTPSGAIPSLGKGPYEIIIFTDYFCPPCYRIDTKAEPLLKELLATQ
                     RVKLTFVDVPFNRSTPVYAKYYLYAVQAHSDATSVFRVRKILFDAAQSKKIQKEADLV
                     AYLKNQKVAWKTMDEKSVFPSLTAVIQKNDIRATPSCVIKYSNKDSQKLIGDVEIWKG
                     LTELKARLSAGNK"
     gene            complement(2963..>3643)
                     /locus_tag="PLG98_11430"
     CDS             complement(2963..>3643)
                     /locus_tag="PLG98_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOE33919.1"
                     /translation="IFRKDDAEHETGELFLPEEEGNGRALWQDALYMLTMVLILVFAS
                     FARPTENDGIVWQMIFSVKWYLVIALVLVLAVMLKKWFVKEELKGWVDSTWGFAKQIL
                     PLLLGGVLVAGFLLGRPGHPALIPEQYIQGLVGGNSLWANFFASIVGAFMYFATLTEV
                     PILQGLIGSGMGQGPALALLLAGPALSLPSMIAIAGIMGIKKTSIYCIIVVILSTIAG
                     MTYGWMVA"
BASE COUNT         1041 a          793 c          822 g          987 t
ORIGIN      
        1 attggcgtcc ccacgcggat tcgaaccgcg gttacaggcg tgaaaggccc gtgtcctagg
       61 cctctagacg atggggacgt ccttaagccg tttcaagcaa gttgtgtctt atatattgat
      121 aaaatcagaa tgtcaagcgt tgactcaaaa aaataacgga aagaatccgc catttttttg
      181 ttgttttaaa tacccggtta ttatcagatt ttcccgctgt ctgtcgctat atatcaatcc
      241 ataaaaatag gaaaggcgga tcatctttga accgcctttc ctaagtgatt tatgctgaac
      301 ggcaatgttt atttatgccc ggcttttacg aaatcctcgg ccatcttgtt gtacttggag
      361 gttttggccg cttctatata ttttgatttt ttcaccatct tcatcatgtt ctgtaaagtt
      421 ttaaagggtg tcgatttgat tttgctgttg gcaattccgg atgggacgcc ggatccgaga
      481 ttcggatcga tcataaacgt gaccctggtg cgttcccggt ctacccactg gagagtgaac
      541 agtgagtaaa atgaaggcat ccggacatat cccttgccgg aatcataggg cagttcattt
      601 gtggagtcgg cataaaccag gttggttccg ttttgcatat caatcacggt tttacttttc
      661 agaatcatat cccggtctgt caacagatca atatgctgac ggaaccagaa gataaaaaca
      721 tccttttcat catcgaccgt tttcaacatt tttgttgctt tgcagtcgtg catccattcg
      781 ggatagttgg ggatgtctct caaaattgta ccgatcactt ctatgcgtgc aggaataagg
      841 catgtggctt tggcggcaat gtaatctttg ccgggaactg cgctggtata gatttggcaa
      901 ttgtcttctg tgtcaacgag tttccattga tatttatcag cggaaaaaca ggttacggca
      961 gtcatcaaaa gcatcagcac gacggtaata cagacatttc ttttagacat aaaaatcctc
     1021 ctgtagtgta tttttaatac ccttcttctg ctcttcttat gcggtgatgt tgatgtggtg
     1081 tatcgtttcc ttacacggtg cggttgagat tgtcaagaaa tatggaagcg gattgaacca
     1141 cgtgaaaact tatgatataa ggtaggcaca ctcattcgtc gcaaatttga ttcctgaata
     1201 taaaaaatgt aaggaggtct atgcgcgatg tcagaagaaa tccacaaaaa aagtcataaa
     1261 gaaccggatg agaagcacct ggacaaaatg acggtcaaag aacttcggga aattgcgtcc
     1321 gaaattcctc atgagaaggc tgttcacgag atgaaaaaag aggaactcat cgcctttata
     1381 aaggaggccc gggggattaa agatgacgag ccggtcaaga agaaacatat cggcaaagtc
     1441 aaaatgacca aacccgaatt aaaagctaaa atacgagagt gcaaggcatc aagattaaaa
     1501 gcgctggaat cgaaggagac tgaaagagcc gtctggttca gacatcagat cagtcaattt
     1561 aagaagaaat tgcggcgtat cgcaggggct tgatttgcac tgctggaagg tgtcggcgtc
     1621 acggtgtgat tgcccatgca aatcgcacat atgatctttt gcgccgtgcg ggatcctaca
     1681 atgcgtgaat tggattgtgc cggaaaagat tccggcacaa tgttttttaa atttgggtct
     1741 tggcaaagag tatcgggtgt ttatatggac ttcgggacat cgctcaggtc atcacttgtt
     1801 tccggctgat aggcgtgcct tcaattccgt caatcccttc catatttcta catcaccgat
     1861 aagcttttgg ctatccttgt ttgaatattt tatgacacat gacggtgtcg cccggatgtc
     1921 atttttttga atcacagccg tcagcgatgg gaaaacggat ttttcatcca ttgttttcca
     1981 cgcaactttt tggtttttta aatatgcgac aagatctgct tctttctgaa tttttttgga
     2041 ttgcgccgca tcaaaaagga tcttacggac tcgaaaaaca cttgttgcat cggaatgggc
     2101 ttgcaccgcg tacagataat atttagcata aacaggtgtc gatcggttaa aggggacatc
     2161 aacaaatgtc agttttaccc gctgggtggc caataattct ttcagcaggg gctcggcttt
     2221 tgtatcgatt cgataacagg gcggacaaaa atagtcggta aagataataa tttcataggg
     2281 ccctttgccc agcgaaggaa tggcaccgga cggcgtttga ccataagccg gtgtgacgga
     2341 tccgttgaag gtcaaaaaaa cggttaaata tcccagtaaa ctgaaaatca gcaggggcaa
     2401 tttatgaatt ttgaacatcg gaaaatcaac ctccccgaga aaatacagcc acatccgacc
     2461 gcgtttttcc cgccatgcgg atggaacttc atagttaatg ataaatgaca aaattaacat
     2521 taccgaaaat gcaagacaaa acgggcaata gacattattt tgtatctgaa aagcgtataa
     2581 atgaacctcg acgccaagac cggcagccag aagagcccgg acaaaaggtg tctgtttaaa
     2641 agcggcgaag attatgatga tggccatata agcgataccg acccatttga gatcgattcc
     2701 cagcaggtcg cctctcagat agctgcactc tgtatcgcaa taatcataat aggccatcag
     2761 aacgatgccg atcagcgaca acgcaatggt aataatattc cgatattttc caataaaatc
     2821 atttacattt gtcatgcctg atgtcctcaa taccttatgt tttattttag ctcaagaaaa
     2881 atatttttca agatatgttg agcatatttt atcgttaatc ttttgtcccg acaatttggc
     2941 gtgttcatcg cctgcggatt tttcaggcaa ccatccatcc ataggtcatg ccggcaatgg
     3001 ttgagagaat gaccacaatg atgcaataga tggacgtttt ttttatcccc atgatgccgg
     3061 cgatggctat catactgggc aatgaaagcg ccggtcctgc cagaagaaga gccagcgccg
     3121 gtccctggcc cataccggaa ccgatgagtc cctgtaaaat aggaacttcg gtcagtgtgg
     3181 caaagtacat aaaagcgccg acaatggaag caaaaaagtt tgcccataac gagttgccgc
     3241 cgacaaggcc ttgaatatat tgctcgggaa tcagtgccgg atgaccggga cgtcccaaaa
     3301 gaaagcctgc caccagtact ccacccagca aaagcggcag tatctgtttg gcaaaccccc
     3361 aggtggaatc cacccatcct ttgagttctt ccttgacaaa ccatttcttg agcatcaccg
     3421 ccaaaacaag cactaatgca atcaccagat accatttgac gctgaaaatc atttgccaga
     3481 cgatcccatc gttttcggtc ggtcgtgcga acgaagcaaa aacaagaatc aacaccatcg
     3541 tcagcatata aagcgcatcc tgccagagcg cccgtccgtt tccctcttct tcaggaagga
     3601 atagttcgcc tgtttcatgt tcagcatcat ccttgcggaa aat
//
LOCUS       DAOSME010000142         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_27639_length_3642_cov_10.520491, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000142 DAOSME010000000
VERSION     DAOSME010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27639_length_3642_cov_10.520491"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLG98_11435"
     CDS             complement(<1..289)
                     /locus_tag="PLG98_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOE33920.1"
                     /translation="MGRDVHDGEMKRYALDKIKDFKATKTCFKCIPATLDGDLKNSAN
                     IWFAVERTIEILILVDQSCADYFKRRKVFPAQEIKEVKPDGSLIVSFLVGNY"
     gene            complement(392..991)
                     /gene="lpoB"
                     /locus_tag="PLG98_11440"
     CDS             complement(392..991)
                     /gene="lpoB"
                     /locus_tag="PLG98_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOE33921.1"
                     /translation="MKTRPIGILLMIIISFIISGCAATIQHGIGDNETVTGSDWSAKD
                     LKEVADHMANSIARRANAPGSELREGKPRWILARDLRNETDEHVNTRTIMEKVRTKLI
                     NDGIARFVDDQALNDILNQLKFQQSGLFDNKTVVQVGKLVGAKYVLRGAISSIRKRSD
                     RIDIIYYNITLQLVNIQTGEIVWTDEKEIQRLTEKSFFR"
     gene            complement(1054..2973)
                     /locus_tag="PLG98_11445"
     CDS             complement(1054..2973)
                     /locus_tag="PLG98_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE33922.1"
                     /translation="MKSKSPLSYNKYFSFFMLVILLATVGCATMRTPLISAVERRDLA
                     AINRLLDAGANINEPSTGKWRASPLYWATYSCMPDVVDLLLKRGASINVVAPDGGTLL
                     LAASVCGEEMHPVVKSLIEKGDKVNAQDTYQGWTPLISASYYNAPKIAKTLLKGGADV
                     NIKAKDGRTALDYAKYYQHNDIIALLSPGNAALLAKDDSQNVIIPVPKEVATDSVAGT
                     EQKIAAAPVAEPATIPAPVPQNAVEPVKIPENIVKSGYTLEYYAMVTSLASGSFDAAF
                     AHTDKLSKQYLSADEGKKNRYLSLLERGKVALAARKYDLAIADLQEAEKRFLSIEGTI
                     SLTEGFGSILTDDTAMEYEAEMHEKLMISPYLIVAYLGKGDFNGAIVERNRTITKINQ
                     FIEEKPVERAYLENPFARYLTALMYEMEDRKDDAKIEYKKLKWDHEIARLESKTGKTT
                     DLVILMDIGLAPQKFQTKWGPAPIPGVGVVGYAYAEYAPTPTRITTCNINIDGKSAGS
                     AKLLYDLENTIITQYQRSKPDIQAKIMARMTAKAATQFAAKMAIKEVAKNIPFASLFA
                     EVAVDAAGAAWIAAEQADLRGWLSLPKKINYLRVTGLEPGEHTISIDYGCGVETKNIR
                     VEKDKINIAHFVYAK"
     gene            3178..3423
                     /locus_tag="PLG98_11450"
     CDS             3178..3423
                     /locus_tag="PLG98_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOE33923.1"
                     /translation="MNYLAAKSSVSITDLKKNPSAVIKEAEGAPVVILNHNRPSAYIV
                     PADAFEAMLERLDDLEIKKTVRKRRSEKSIPVALVAL"
     gene            3413..>3642
                     /locus_tag="PLG98_11455"
     CDS             3413..>3642
                     /locus_tag="PLG98_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33924.1"
                     /translation="MPYKLAFKESALKEWGKLDRPIREQFKKKLAKRLERLRVEADRV
                     NGTRRQNRGKADAERNEKADQMAKATDEIRQIV"
BASE COUNT          853 a          907 c          844 g         1038 t
ORIGIN      
        1 cgtaattacc gactaagaaa gagacgatga gtgatccatc gggttttact tcttttatct
       61 cctgggcagg aaatacctta cgccgtttga aatagtcggc gcaggattga tcgaccagga
      121 tgagaatctc gatggtcctt tccacggcga accagatatt ggcgctgttt ttcaagtcgc
      181 catccagcgt ggcgggaatg catttgaaac aggttttggt agccttgaaa tccttgatct
      241 tgtccagggc atagcgtttc atctccccgt catggacgtc cctgcccacc agacccctct
      301 gcaggagaaa agttaaaaca tcttgttaca aatccaaatc ttgtgaagct catgttatag
      361 attcgttcta tactccccat tgttattggg gctatcgaaa gaacgatttc tccgttaatc
      421 tttgaatttc tttttcatcg gtccagacta tttcaccggt ttgtatgtta accagttgca
      481 gcgtgatatt gtaatagatg atatcgatac gatcacttcg ttttcgaata ctggaaattg
      541 cacctcttag aacatatttc gcgccaacca gtttgccgac ttgaacaacc gttttgttgt
      601 caaacaggcc ggattgctgg aattttaact ggttcaagat atcgtttaat gcctgatcat
      661 ccacaaatct tgcaatgccg tcattaatta attttgtccg gaccttctcc ataatggttc
      721 tggtgttcac atgttcgtcc gtctcgtttc gcaaatccct ggcaaggatc catcgtggtt
      781 ttccttccct taattctgat ccgggtgcgt tggcacgtct ggcaattgaa ttggccatgt
      841 ggtcagccac ttcttttaaa tctttggcag accagtctga tcctgtgacc gtttcattat
      901 ctccaatgcc gtgctggatc gttgctgcac aaccagaaat tatgaagctt atgatgatca
      961 tcagtaaaat tcctatcggt cttgttttca tgtcttattc gcctgctatt attatttaga
     1021 ttattaagaa gaatgctctc ctatccgcaa tccttacttt gcatatacaa aatgcgcgat
     1081 attgatcttg tccttttcca cacgaatatt cttcgtctcc acaccgcatc cgtaatcgat
     1141 gctaatcgtg tgttcaccgg gctcaaggcc ggtaaccctg aggtaattga ttttcttcgg
     1201 cagtgacagc catccccgaa gatccgcctg ctcagccgct atccatgccg cacctgccgc
     1261 gtctacggcc acctctgcga ataatgacgc gaagggaatg ttttttgcga cttctttgat
     1321 cgccatcttc gccgcgaact gtgttgcggc cttggcggtc attctggcca ttattttggc
     1381 ctggatgtcc ggcttgctcc tttgatactg tgtgatgatc gtattctcaa gatcgtaaag
     1441 cagttttgca ctaccggctg attttccatc aatgttaatg ttgcacgttg taattctggt
     1501 cggtgtcggc gcatattcgg cataggcata tcccacgacg ccaacgcccg gaatcggcgc
     1561 gggcccccac ttggtctgga atttctgagg ggctaatcca atgtccatta aaatgaccag
     1621 atcggttgtt ttgccggtct tgctctccag tctggcaatt tcatgatccc atttcagctt
     1681 tttgtattcg atttttgcat catcttttct atcttccatt tcatacatga gagcggtcag
     1741 gtatcgcgcg aacgggtttt ccagataggc cctctccacc ggcttctctt caataaattg
     1801 gttgattttt gtaatcgtcc ggtttcgttc gacgatggcc ccgttaaaat cccccttgcc
     1861 gagataggca acaatcaggt aggggctgat catcagtttt tcatgcattt ccgcttcata
     1921 ctccatcgcc gtatcatccg tcaggataga gccgaatcct tcggttaatg atatcgttcc
     1981 ttcgatggag aggaatcttt tttccgcctc ctgaagatca gcgatagcca gatcgtattt
     2041 gcgggcggcc agggccacct tgcctctttc cagcaaagac agatacctgt ttttcttgcc
     2101 ctcgtcggca ctcaggtatt gtttggacag cttgtcggtg tgtgcaaaag cagcatcaaa
     2161 ggagccactc gccaggctcg tcaccatcgc atagtactcc agcgtgtagc cgctctttac
     2221 aatattctcc ggaatcttca ccggttcaac cgcattctgc ggaacgggcg ccggaattgt
     2281 tgccggttca gccacgggtg ccgcggcgat tttctgttca gttcccgcaa ccgaatccgt
     2341 tgccacttcc ttcgggacgg gaataatcac gttttgcgaa tcgtcttttg ccagaagcgc
     2401 ggcattgccg ggactcaaga gagcgatgat atcattgtgc tgataatatt ttgcataatc
     2461 gagagccgtt cgcccgtctt tggctttaat attcacatcg gccccgcctt tgagcagtgt
     2521 ttttgctatc ttcggggcgt tgtaataaga tgcgctgatc aaaggggtcc agccctggta
     2581 agtgtcctgg gcgtttacct tatctccctt ttcaatgaga gatttgacga ctggatgcat
     2641 ctcttctccg cagaccgatg ccgccaataa cagagtgccc ccgtcaggtg caacgacgtt
     2701 gatgctcgca cctcttttca gcagaagatc aacaacatca ggcatacagc tataggttgc
     2761 ccagtataga ggcgatgcac gccatttgcc cgtgctcggt tcgttgatgt ttgcaccggc
     2821 atcaagaagc ctgttgatag cggccagatc ccgtctttcc acggcactga tgagaggggt
     2881 tctcatggtg gcgcacccaa ctgttgcaag caagatgacc aacatgaaaa aagaaaagta
     2941 tttgttgtaa gataaagggc ttttgctttt cataattggt ttcttcctgc gtaaaaggcc
     3001 atctctatgg caataacctg ctgcatgaaa acggctgaac tattatcaac agtttccatg
     3061 tttttgtcaa catatgtgat tatgcacata tgtaattatg cccgtaaaat accttgccct
     3121 cgtagccgat atgacatata ttcagagccg aaaataggtt ttattggagg atgagtaatg
     3181 aactatctgg cggcaaaatc gtcggtcagc atcacggacc tgaaaaagaa cccttcggcc
     3241 gtcatcaagg aggcggaggg ggcgcccgtg gtgattctca accacaatcg gccttccgct
     3301 tacatcgtac ccgccgatgc cttcgaggcc atgctggaaa ggctggatga tcttgaaatc
     3361 aaaaagaccg tccgcaagcg acggagcgaa aaatcgatcc ctgtagccct cgttgcccta
     3421 taaactggcc ttcaaggaat ctgccctgaa ggagtggggc aaactggacc ggccgattcg
     3481 ggaacagttc aaaaaaaagc tggcgaaacg gctggagcgt ctgcgtgtgg aagcggacag
     3541 ggtcaatgga acaagacggc aaaaccgcgg aaaagcggat gctgagcgaa acgaaaaggc
     3601 tgatcaaatg gctaaagcaa cagatgaaat cagacaaata gt
//
LOCUS       DAOSME010000143         3617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_27887_length_3617_cov_24.423358, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000143 DAOSME010000000
VERSION     DAOSME010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27887_length_3617_cov_24.423358"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLG98_11460"
     CDS             <1..834
                     /locus_tag="PLG98_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOE33925.1"
                     /translation="KFWKDQGVRRIILARELNLKEIGEIAAAVPDMELEIFIHGAMCM
                     SYSGRCYLSAFRNRRSANEGDCSQPCRWEYKLYESTRPDDPLILEEDERYSYLLSSKD
                     LCLIEHLPDVLAAGVTSLKIEGRMKSAYYVAVVTRTYRQALNALLHEKDEYKFRREWS
                     DELGTISNRGYTTGFVFAEEKINETNPDIKYAQTHEPAGIVLQYDAAQKRMLIGVRNR
                     IVAGSPIELILPDETVLIDTRILMDENGQSRTEAHNDQRIYLPVEKEAPVGAILRQKA
                     K"
     gene            complement(1084..1623)
                     /locus_tag="PLG98_11465"
     CDS             complement(1084..1623)
                     /locus_tag="PLG98_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE33926.1"
                     /translation="MTKNFKSTQPGIPYFVTFTVVEWLNVFTKTEYLEILWDSLQYCR
                     KEKGLRLFGFVFMTNHVHLIISAHDENIKLWQIIRDFKKFTAQQIIQVMQKEENRHWI
                     LDVMKNVGKQNNANTQYQFWIQDDGAEEIQSDKFFIQKLNYIHDNPVRARIVTDACGY
                     VWSSARLYEKDDFSLIDRI"
     gene            complement(1826..2965)
                     /locus_tag="PLG98_11470"
     CDS             complement(1826..2965)
                     /locus_tag="PLG98_11470"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE33927.1"
                     /translation="MTIDEKYKKAGEIISTAGGTPLPVNDTLIKLLKFFIMEDELDFI
                     AAFAEKKSQTMEQLKQSSGLSEDEINKKVKDLAKRGVIFNQPNSKGIMVFRLLPLFNV
                     GTFEYNFMGKLVIDDRNKEIAGLFKQMFADLNNMVQDNYDVIVPMLMAGPAIDRTVPV
                     FENKPKSEPVKIVINEKLEEAKDRVVPSQRVEELIQKFDEIALGHCFCRHYEDIKGTP
                     CKQTDARETCFTFGKSARFTSEQGFSRLITKEEALKVLKEAEEAGLVHKAYHPNFDTT
                     KDETSICNCCRCCCGNGVDHMIAPIANVTSFMAVVNDDLCIGCGTCVKKCHTYAAYLN
                     DDKKARRQEDRCIGCGVCAHFCPQNAITMVKGAERIVRIQPPKRK"
     gene            complement(3021..>3617)
                     /gene="hypB"
                     /locus_tag="PLG98_11475"
     CDS             complement(3021..>3617)
                     /gene="hypB"
                     /locus_tag="PLG98_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOE33928.1"
                     /translation="NLQKTKTMLVNLMSSPGSGKTSLILQTIAGLKDSVKLGVIEADI
                     DSMVDAEKVAAKGIRAVQLKTGGFCHLDATMVTQGIDALSVGDLDLVIIENVGNLVCP
                     AEFDTGAHKNVMILSVPEGDDKPLKYPLMFSICDVLLVNKIDYLSLSDFDMEAMRKRV
                     LALNPKIEIIEVSCKTGAGIDQWVGWLKKEVAAFKNLK"
BASE COUNT          891 a          915 c          820 g          991 t
ORIGIN      
        1 aaattctgga aagatcaggg cgtgcgccgc attattctgg cgcgggaact gaatttaaaa
       61 gaaatcgggg aaatcgccgc cgctgttccc gacatggagc tggaaatttt cattcacggc
      121 gcgatgtgca tgtcctactc cggccgctgc tatctctcgg cctttcgtaa tcgtcgcagt
      181 gccaacgaag gcgactgctc ccagccctgc cgctgggagt ataaactcta cgaatccaca
      241 aggccggacg atcctctgat tctggaagaa gacgagcgct acagctattt gctcagttcc
      301 aaagatttat gcctcatcga acatctaccc gacgttctgg ccgccggtgt gaccagtctc
      361 aaaatcgagg gccggatgaa atcagcttat tacgtggccg tggtcacgcg aacataccgg
      421 caggcgctga atgccctttt gcacgaaaaa gacgaataca aattcaggcg ggaatggagt
      481 gatgaactgg gcaccatttc caaccgcggt tatacgacag gctttgtttt tgccgaagaa
      541 aaaataaacg aaacgaatcc ggacatcaaa tatgctcaga cccacgaacc ggcgggcatc
      601 gttttgcagt atgatgccgc ccaaaaaaga atgctgatcg gcgtccgcaa tcgtatcgtt
      661 gccggaagcc caatcgagtt gatcctgccc gatgagacgg ttttaatcga cacgcgtatt
      721 ttaatggatg aaaacggaca atcgcggaca gaagcccata acgatcagcg gatttacctg
      781 cccgtggaaa aagaagcgcc ggtcggcgcc atcctgcgac aaaaggcaaa ataaaacaga
      841 accattcgca actagatcaa tccatcaatt ttgtcatacc ggcggaggcc ggtatccagt
      901 tttctattta taacttggtc cccggcccac cgttaggtcg agtctttttg actcgaccgt
      961 gataatttga cagccaagtc aaaaagactt ggctgaacca agagggaact gcgccgccgt
     1021 taggtcgagt cttttcgaca aattgttagg tcgagtcttt tcgactcgac cttcaaacac
     1081 aactcaaatt ctatcaatca ggctgaaatc atctttttca taaagccttg ccgaactcca
     1141 cacataaccg caggcatcgg tgacaattct cgcgcgcacc ggattgtcat ggatgtagtt
     1201 cagtttttga atgaaaaact tatcgctttg aatttcttcc gcaccgtcgt cctgtatcca
     1261 gaattgatat tgcgtattgg cattgttttg ttttccgaca tttttcatca cgtcgagaat
     1321 ccaatgcctg ttttcttctt tttgcataac ctgaattatt tgctgagcgg taaatttttt
     1381 aaaatccctg atgatctgcc agagtttaat gttttcgtca tgagcgctga tgatcagatg
     1441 cacatggttg gtcataaaaa caaacccgaa aagccggagc cctttttctt tgcggcaata
     1501 ttgcaggctg tcccagagaa tctccaaata ctccgttttt gtaaaaacat ttaaccattc
     1561 aacgacggta aatgtaacaa agtaaggaat accgggttgt gtggatttga agtttttagt
     1621 catggaaggc ttttagcata gagggttact tcactcgagt cgaaaagact cgactgaaca
     1681 atgacacaga agggttaatt cggtcgagtc gaaaagactt gactgaacaa taagcaaaac
     1741 aatagacaaa ccgttgggtc gagtcaccgt taggtcgagt cttttcgact cgacctaata
     1801 atgagttttt tccgcaccgg acacgttatt tccgtttcgg cggctggatt cttacaattc
     1861 tttctgcgcc tttcaccatg gtgatcgcgt tttgcggaca gaaatgcgcg catacgccac
     1921 agccgatgca gcggtcttcc tgtctgcggg cttttttatc gtcattcaaa tacgcggcgt
     1981 aggtgtggca tttcttcacg caggtgccgc agccgatgca gagatcgtcg ttcaccactg
     2041 ccataaaact ggtcacattg gcgatgggcg caatcatatg atcaacgccg ttgccgcagc
     2101 agcaccggca gcagttgcag atgctggttt catccttggt ggtgtcgaaa ttgggatgat
     2161 aggccttgtg caccaggccc gcttcctcgg cctctttcag gaccttcagg gcttcttctt
     2221 ttgtaattaa tcttgaaaat ccctgctcgg acgtaaaccg cgccgacttg ccgaacgtaa
     2281 agcaggtttc cctggcatca gtttgtttgc agggcgttcc cttgatatcc tcgtaatggc
     2341 ggcagaagca atgcccgagg gcaatttcgt caaacttctg aatgagttct tcaacccgtt
     2401 gggacggcac tactctgtct ttggcttctt cgagtttttc attgatgacg attttaaccg
     2461 gttcactctt gggtttattt tcaaagacag gcaccgttct gtcgatggcc ggtccggcca
     2521 tcagcatcgg gacgatcaca tcgtaattgt cctgaaccat attgttcaaa tccgcaaaca
     2581 tctgcttgaa caatccggca atttccttat tcctgtcgtc aatgaccagt ttacccatga
     2641 aattgtattc gaacgtgcca acgttaaaca gcggcagcaa acggaaaacc atgatgcctt
     2701 tgctgttggg ctgattgaaa ataacgcccc ttttcgccag gtctttcact tttttattga
     2761 tctcgtcttc ggacaggccg ctggactgct ttaactgttc catggtctgg gatttctttt
     2821 ccgcaaaagc ggcaataaaa tccagttcgt cttccatgat aaaaaacttc agaagtttga
     2881 tcagcgtatc gttgacggga agcggcgtac ctcccgctgt gctgataatt tctccggctt
     2941 ttttatattt ttcatcaatc gtcatgtttt ctgccctctc ttgttttttg tgaatgtttg
     3001 tcccgttaat gataggcaat ctatttcaaa tttttaaacg ctgcaacttc tttcttcagc
     3061 cagccgaccc attgatcgat tcccgctccc gttttgcagg aaacctcgat gatctcaatt
     3121 ttgggattca acgccagaac ccgtttgcgc atggcttcca tatcaaaatc gctcagcgac
     3181 aaataatcaa ttttattgac cagcagcaca tcacagatgg aaaacatcag cgggtattta
     3241 agcggtttgt cgtccccttc gggcacactc agaatcatca catttttatg ggcgccggta
     3301 tcgaattccg ccgggcagac cagattgccc acgttttcga taataacaag atccagatcg
     3361 cccacgctta aagcgtcaat gccctgcgta accatcgtgg catccagatg acagaaccct
     3421 cccgtcttga gctgcacggc gcggattccc ttggcggcga ctttttccgc gtccaccatc
     3481 gaatcgatat ccgcctctat cacgcccagt ttcacgctgt ctttcaaacc ggcaatggtt
     3541 tgcagaatca ggctggtctt gcccgacccc ggcgaggaca tcagattgac cagcatggtt
     3601 tttgtctttt gcagatt
//
LOCUS       DAOSME010000144         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_28023_length_3603_cov_18.931229, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000144 DAOSME010000000
VERSION     DAOSME010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28023_length_3603_cov_18.931229"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLG98_11480"
     CDS             complement(<1..297)
                     /locus_tag="PLG98_11480"
                     /inference="COORDINATES: protein motif:HMM:NF023834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HOE33929.1"
                     /translation="MAHSNWLYQTRDIMFQIKEWLGVEKLLALDAYKDYFGMDDIDSF
                     LDVNFKVCRDVMCPANKDADEPGAKFVGGNEHAVTTPDSFKNVYKTIMDAELGPQ"
     gene            complement(495..1421)
                     /locus_tag="PLG98_11485"
     CDS             complement(495..1421)
                     /locus_tag="PLG98_11485"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOE33930.1"
                     /translation="MSQKKNFRKKLLNRGHGYPFQISISGVDRRQSFPYTAGQFILFR
                     DVFGMTVNVKIEDHLLLIEINRPEKYNAMTQGMYHDLARAYFRLDQEEDARVGLVYAV
                     GPHFTSGLELTDWGGTFDAGKGFPFVAENEIDPFYVMSQRRCRKPVVMAVQGYCYTWG
                     FEMMLSTDIRVAANDTRFAMLEVKRGFFPAAGATLRLPREIGWANAMRYMLTGEEMSA
                     AEAYRLGLVQHLTEPGKQLDLALEIARSVARAAPLGVQGALASCRKAAEEGDKAALAV
                     MFRDVAQVMKSEDMKEGLQSFVERRTAVFKGK"
     gene            complement(1432..3150)
                     /locus_tag="PLG98_11490"
     CDS             complement(1432..3150)
                     /locus_tag="PLG98_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOE33931.1"
                     /translation="MKFRKMDRFQKVVKGCLVFFFIALVSCSGGNQDSAYTLTLVHLN
                     DTHSHLEPVAVNFWVNDVKTTVQAGGFARLKTAIDRMRAIWPDLLLLHGGDAVQGTLY
                     FTLFKGEVEFDFLKLLGIDAMTFGNHEFDRGCQPIPDWITRSGFPWLSANIDFTGEPA
                     IAVLVAPYLIKTVSGERVAIIGVTTETTPQTTLDVGGAVFHDAVASTRAQVAELTAMG
                     INKIILLSHLGYQEDLALARQVAGIDIIVGGHSHSLLGDDQGLSLIGLTPAGPYPSEQ
                     MAPGGGKVLVLQAWQWGHVLGRISVRFTPTGEIISHQARMTIPVGDVFVQNGATVEPG
                     SQAYGDILAALDAAGSARIYPEDPQVKARLEPYATQVAVFRQEKVATALNLIARGINS
                     GPGPLACDSMLGSVPDTRAALLNYGGVRRDLEAGDITVGDILEVMPFGNTLMLLDLTG
                     AEIKTALEEGIDFLIKNYGSDYMPYVAGMKFTVYVSAGAGERVSDLTIRDAEGVYRAA
                     EASKIYRIVTIAFVAGGGDGFATLKKATGFRSDTGIIDSDAFRDYLHRLVAVENPTDE
                     RIVVVH"
BASE COUNT          912 a         1041 c          853 g          797 t
ORIGIN      
        1 ctgggggccc agctccgcat ccataatggt tttataaaca ttcttgaacg agtcgggggt
       61 cgtcacggca tgctcgttgc cgcccacaaa tttcgcgccg ggttcatctg cgtctttgtt
      121 ggccggacac atcacgtcgc ggcagacttt aaagttgaca tcgaggaagc tgtcgatgtc
      181 gtccatcccg aagtaatcct tataagcgtc aagggccagg agcttttcca cgccaagcca
      241 ttccttgatc tggaacatga tgtcccgcgt ctgatacaac cagttactgt gtgccatctt
      301 caattcctcc tgatgaaaat aaaaggcata gaatgaaacg aatcatcttc cccgtaagat
      361 taaaattcct cttacccgat tcgtgtacat ggcccctgcc agagcatgga ttcatgcaaa
      421 gccgttgttc attgactgcc gttagaaaat tatcgtcctg tccgccttct ttttttacga
      481 ccgggccctc cgcgttattt ccccttaaat accgcggtgc gccgctcgac aaaagattgc
      541 agaccctcct tcatatcttc acttttcatg acctgggcga catcccgaaa catgaccgct
      601 aaagccgctt tgtcgccttc ctcggcggct ttgcggcacg aggccagagc cccctgcaca
      661 cccagcggcg cggcccgcgc cacgctacgg gcgatttcca gcgcaaggtc caattgtttg
      721 cccggctccg tcagatgctg aaccagaccc aggcggtaag cctcggccgc gctcatttct
      781 tccccggtca gcatgtaacg catggcattg gcccagccga tttcgcgagg cagcctcagc
      841 gttgccccgg cggccggaaa gaaaccgcgt ttgacttcca gcatggcaaa acgggtatcg
      901 ttggctgcca cccggatatc ggtactcagc atcatctcga aaccccaggt gtaacaatag
      961 ccctggacgg ccatgacaac cggtttgcgg cagcgccttt ggctcatcac ataaaacgga
     1021 tcgatttcat tttctgcgac aaaaggaaat cctttgcccg cgtcgaaggt cccgccccag
     1081 tccgtcagct ccagaccgga tgtgaaatga ggtcccaccg catagaccag acccacacgc
     1141 gcatcttctt cctgatccag acgaaagtaa gcccgggcca gatcgtgata catgccttgt
     1201 gtcatggcat tgtatttttc aggcctgttg atttcaatga gcagaagatg atcttcgatt
     1261 ttaacattga cggtcatgcc gaatacgtcc cggaataaaa taaactgacc ggcagtatat
     1321 ggaaaggatt gacgcctgtc aacgccggaa attgaaatct gaaacggata accgtggccc
     1381 cggtttaaga gttttttccg gaagtttttt ttctgcgaca tttttcactt cttaatgaac
     1441 aaccactatg cgctcatccg tcgggttttc cacggcaacc agacgatgca gatagtcccg
     1501 aaacgcatcg ctgtcgataa tccccgtatc actgcggaaa ccggtcgctt ttttcaaggt
     1561 cgcaaagccg tcgcctccac cggcaacaaa cgcaattgtt acaatgcgat agatcttcga
     1621 cgcctccgcc gcccggtaga cgccttccgc atcccggatg gtcagatcag acactctctc
     1681 tcccgcccca gccgacacat agacggtaaa tttcataccg gccacgtaag gcatataatc
     1741 agagccataa tttttaatca gaaaatcgat gccctcttcc agggcggttt tgatttcagc
     1801 cccggtcaga tcgagaagca tcaatgtatt gccaaacggc atgacctcca gaatatcacc
     1861 gacggtaatg tcgcccgctt caagatcgcg ccgaacgccc ccgtagttga gaagcgccgc
     1921 ccgggtatcg ggtacggagc ccagcatgga atcgcaagcc agcggaccgg gaccgctgtt
     1981 gatgcctcgg gcaatcaggt tcagtgcggt cgcaactttc tcctgccgga acactgctac
     2041 ctgcgtggcg tagggctcca accttgcctt gacctgcggg tcttccggat aaatccgtgc
     2101 cgaaccggcg gcgtcaaggg cggcaaggat atccccgtaa gcctgcgaac cgggctcaac
     2161 cgtcgcgccg ttctggacaa aaacatcgcc cacgggaata gtcatccggg cctgatgact
     2221 gatgatttcg cctgtcgggg tgaaccgcac ggaaatccgg cccaggacgt gtccccactg
     2281 ccaggcctga aggaccagaa ccttaccccc gccgggcgcc atctgctccg agggataggg
     2341 acccgcaggc gtcagtccga tcagcgaaag tccctgatca tcacccagca gcgaatggct
     2401 gtgacctccg acaataatat caatgcctgc aacctgccgg gccagcgcca gatcttcctg
     2461 atagcccaga tgcgacaaaa gaataatctt attgatcccc atcgcggtca actcggccac
     2521 ctgggcccgg gtactggcca ctgcgtcatg aaacacagcg ccaccgacat caagagtggt
     2581 ctggggcgtt gtttccgtgg tcacaccgat aatggccacg cgttcgcccg aaaccgtctt
     2641 aataaggtaa ggcgcaacca gaaccgcaat ggccggttcg ccggtaaagt cgatgttggc
     2701 ggaaagccac ggaaatcccg aacgggtgat ccagtcggga atcggctggc agccccggtc
     2761 aaattcatgg ttgccgaaag tcatggcatc aatgcccaga agcttcagga aatcgaattc
     2821 aacctcccct ttgaaaagcg taaaatacag cgttccctga accgcatctc caccatggag
     2881 caacaaaaga tccggccaga ttgcgcgcat ccggtctatc gcggtcttca gccgggcaaa
     2941 gccccccgcc tgcacagtgg ttttgacgtc attgacccag aaattaaccg ccaccggctc
     3001 cagatgggaa tgggtatcgt tcagatgcac cagcgtcagc gtataggcgg aatcctgatt
     3061 tccgccggag caggacacaa gcgcaatgaa aaaaaagaca aggcaaccct ttacaacttt
     3121 ttgaaaccgg tccatttttc ggaatttcat cgggcatcgc tcccttaaga aaaaatcggc
     3181 tctccgctct tgcgtgggcg tcgggctaac cctatatcgt caagtgcaat ggttttcttg
     3241 actaaaaaag cccgattcca tcaggccttt ggagaagtag ccctcgcaga caaaacactc
     3301 tgaaaaacct acatgcagta aacccgtccg tcgcaacggg gggggcgcgc agaatttcgt
     3361 cgaaaccata ataatatcaa aatgattaga ctcatggacg aagggtattt ttattccctt
     3421 taatattcca cttgacagat tatggtttaa aaccaaaact tacaaaaaaa ctattatatt
     3481 tggcagaaaa actttcgaaa atattaaagc attaaatcca aataacaatt acctagttct
     3541 tacaaaacaa aaaaatttta aagtttttga aaaaaatata aaaataatag atacccttga
     3601 aaa
//
LOCUS       DAOSME010000145         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_28494_length_3559_cov_20.280822, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000145 DAOSME010000000
VERSION     DAOSME010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28494_length_3559_cov_20.280822"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLG98_11495"
     CDS             <1..291
                     /locus_tag="PLG98_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOE33932.1"
                     /translation="VQKHYYKHLKGEETSSNSMALIFAWSGGLRQRGHMDGTPDVVTF
                     ADTLEEAAIATVERGIMTGDLLALAEKKASNKKVNTEGFIDAIAATLQEKLP"
     gene            288..1382
                     /gene="rlmN"
                     /locus_tag="PLG98_11500"
     CDS             288..1382
                     /gene="rlmN"
                     /locus_tag="PLG98_11500"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOE33933.1"
                     /translation="MTGRAEHSLPNLLDFTQEELQDFISVLGKEKFRAQQIMKCLYQS
                     GYTSFDEMTTLSRDFRTGLSQIARISRPHIVKVQTSADGTKKALLRLKDGLFIESVLI
                     PGKNNWTICVSTQAGCAMDCKFCLTARQGFQRNLNPSEITGQILTLKHELPGCPDIKN
                     IVMMGMGEPLANYDNVVTAIKNITGDFGLGFSNRKVTVSTCGLAPQIIRLGKDICINL
                     AVSLNAHDNDTRSGLMPVNKKYPLEILLDACRQYPMPGRRLLTFEYILMDGINSSVQD
                     AKKLAALLKGQRCKLNLIVFNEYPGSPFRSPSPKTVEAFQKILIDAHYTTMLRASKGS
                     DILAACGQLSGTYNDQVSHCSFQKGSKFLT"
     gene            1472..2032
                     /locus_tag="PLG98_11505"
     CDS             1472..2032
                     /locus_tag="PLG98_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1904..1906,aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOE33934.1"
                     /translation="MRFDSTLKEVKMMNVFREKTWSPYAAGALAGLLLILSVFITGKY
                     FGASTTFVRVAGYVEQTVTPDKIAGMEYFIKNKAKIDWQFMFVVGIFFGALAAAWLSG
                     EKRMVAVPKMWEERFGPSRAKRWTAAFLGGIVLMFGARLADGUPSGHGLSGLAQIAFS
                     GYVSLICFFLGGILIARIVYGGNHHE"
     gene            2025..2552
                     /locus_tag="PLG98_11510"
     CDS             2025..2552
                     /locus_tag="PLG98_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOE33935.1"
                     /translation="MNELVIGAVTGILFGFVMQKAQVIRYDRQLGALRFKDMTIVKFM
                     LTTILVAMVGIYLLFDLGLVKLSIKPLILGGNVLGGLIFGLGWGVVGYCPGTAMGALG
                     EGRYDAVFGLLGMILGAGIYAELYPTMKTTVLTWGDFGKITLPAVLGINHWFVIIIVA
                     AAFLGLLGWFEKKGL"
     gene            2809..3300
                     /locus_tag="PLG98_11515"
     CDS             2809..3300
                     /locus_tag="PLG98_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33936.1"
                     /translation="MKNTNFLIIILLTVFLLSAADSWAKNWTLLGTDPNGTEYYKPSD
                     VKKVTKNIVHVRTKIIYNERGKFRNFSRLTKTEKNLRNPYILNFEMKWIEFNCTTRQY
                     RILSGGIYDKRGKLISDLPPSGKKWSRIPAGSPAEKLMDILCGNTDVTKNNGSKNKKV
                     DPE"
     gene            3339..>3559
                     /locus_tag="PLG98_11520"
     CDS             3339..>3559
                     /locus_tag="PLG98_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33937.1"
                     /translation="MMKKFFLFLAILFLFSCAQIVRNPVKYDQQVFINHYDKMFSAAM
                     NQGTRMSYKIDYQNREYGLLKMSRKVGVST"
BASE COUNT          967 a          828 c          839 g          925 t
ORIGIN      
        1 gtacagaagc attattacaa acatctcaaa ggcgaagaaa cgtccagcaa ctccatggcg
       61 ctcattttcg cctggagcgg cggtttgcgc cagcgcggtc acatggacgg aacaccggac
      121 gttgtcacgt ttgccgacac actggaagaa gccgccattg ccaccgtcga gcgcggcatc
      181 atgaccggcg atcttctggc gctcgctgag aaaaaagcgt ccaataaaaa agtgaatacg
      241 gaaggtttta tcgacgctat tgccgcaaca ttacaagaaa agcttccatg accggtcgcg
      301 ctgaacattc gcttcccaat ctgctggatt ttacgcagga ggaattgcag gactttattt
      361 ccgttctggg caaggagaaa ttccgcgcac agcagatcat gaaatgtctg tatcaatccg
      421 gctacacgtc tttcgatgaa atgaccaccc tttcccgcga tttccgcacc ggtctttctc
      481 aaatcgcccg catcagccgc ccgcacatcg tcaaagttca aacatccgca gacggaacaa
      541 aaaaggccct gttgcgcctg aaagacggac tttttattga aagtgtcctc attccgggga
      601 aaaacaactg gacgatctgc gtatccaccc aggccggatg cgccatggac tgtaaattct
      661 gtctgacggc gcgccagggc tttcaaagga acctgaatcc ttcggaaatt acgggacaga
      721 tcctgactct gaaacatgaa ttgcccggat gcccggacat caaaaatatc gttatgatgg
      781 gcatgggcga accgctggcc aattacgaca atgtcgtcac ggcaattaaa aatatcaccg
      841 gcgacttcgg gctgggattt tccaaccgca aagtgaccgt ttctacctgc ggtcttgccc
      901 cgcagattat ccggctggga aaagatattt gcattaatct ggctgtatcg ctcaacgctc
      961 atgataatga caccagaagc gggctaatgc cggtcaacaa aaaatacccc ctggaaatcc
     1021 tgctggatgc ctgccggcaa tatcccatgc ccggtcgtcg attgctgact ttcgaataca
     1081 ttttaatgga cgggataaac tcttccgtgc aggatgctaa aaagctggct gcattgctga
     1141 aaggacagcg ctgtaaactg aatctgatcg tttttaacga atatccgggc tcccctttcc
     1201 ggtcgccatc gccgaaaaca gtggaagctt ttcagaaaat cctgattgac gcccactaca
     1261 caaccatgct tcgtgccagt aaaggaagtg atattctggc cgcctgcggg caattgagcg
     1321 gaacctataa cgatcaggtc agtcattgtt cctttcagaa gggatcaaaa tttttaacct
     1381 gattgccgta cgttctgatt ggacgttttc agctattgac actgaatatc aaatccttta
     1441 taagtattga aaagttttgt ttttttacgc aatccgcttt gactcaaccc tcaaggaagt
     1501 aaagatgatg aatgtctttc gtgaaaaaac gtggtcaccc tatgccgccg gcgccctggc
     1561 gggacttttg ctcatccttt cggttttcat cacagggaag tattttggcg cctcaacaac
     1621 ttttgtccgc gttgccggtt atgtcgaaca gaccgtaacg ccggataaaa ttgccggcat
     1681 ggaatacttc atcaaaaaca aagcaaaaat cgactggcag ttcatgtttg ttgtcggaat
     1741 tttcttcggc gctttggccg ccgcctggct atccggagag aagcgcatgg ttgccgtgcc
     1801 gaagatgtgg gaagaacgat tcggtccgtc gcgcgcaaaa cggtggaccg ccgcatttct
     1861 tggaggcatc gtcctgatgt tcggcgcccg gcttgccgac ggctgaccga gcggacacgg
     1921 actgagcggt ctggctcaga tagccttcag cggttatgtt tcattaattt gtttctttct
     1981 gggcggcatt cttatcgccc ggattgtata tggagggaat catcatgaat gagctggtta
     2041 tcggggcagt cacgggaatt ctcttcggct ttgtaatgca gaaagcccag gtcatccggt
     2101 atgaccgcca actgggcgct ttgcgtttta aagatatgac tattgttaaa ttcatgctga
     2161 ccactatact ggtcgccatg gtggggattt atctgctttt tgatctcggg cttgtcaaac
     2221 tctccatcaa gccgctcatt cttggcggaa acgtattggg cggtctgata ttcggccttg
     2281 gctggggagt ggtgggatat tgtcccggaa cggcaatggg cgccctgggc gaaggccgct
     2341 atgatgcggt ctttggttta ctgggaatga ttctgggagc cggcatttac gccgaactat
     2401 atccgacaat gaaaacaacc gttctcacct ggggcgattt cggaaaaatc accctgccgg
     2461 ccgtactggg gatcaatcac tggtttgtta ttatcattgt ggctgcggct tttttagggc
     2521 ttttgggctg gtttgagaaa aaaggtttat aaaacgtttt tttacaaaaa ccttgtttgt
     2581 atcacagccg gtcgccggct gtgatacaaa caaccgctca ccaaagatgc ttcttcaaca
     2641 atcaatttat ctttccctct tttaaaaaaa ctgtttttgc gttgcctgcc ttccacaata
     2701 aaagaaaaac gccggatttt cctctttaaa aaaataggat tagtgctatg atagaaaaat
     2761 cgaatgacag gtacaaaaca ggaattccat tttccgggag aagaaacgat gaaaaatacc
     2821 aattttctaa tcatcattct tctgactgtt tttcttttat cggctgccga ttcctgggcg
     2881 aaaaactgga ctttactggg aacagaccct aatggcacgg aatattacaa gccaagcgac
     2941 gttaaaaaag ttacgaagaa tattgtgcac gtcagaacga agattatcta taacgaaagg
     3001 ggaaagttca ggaatttctc ccggctgaca aaaacggaga aaaatctccg caatccctac
     3061 attttaaatt ttgaaatgaa atggatcgaa ttcaactgca caaccaggca atacaggata
     3121 ctgtcaggcg gtatttatga caaaagagga aagcttattt ccgatttgcc gccatccggt
     3181 aaaaaatgga gcagaattcc cgccggttca cctgcggaga aactcatgga tatcctttgc
     3241 gggaatacgg atgtgactaa aaataatggt tctaaaaata aaaaggttga tccagagtag
     3301 atgactgaaa attctattga agcaagggag acaacattat gatgaaaaaa tttttcttat
     3361 ttctagccat actatttttg ttttcttgcg cgcagattgt cagaaatccg gtcaaatacg
     3421 atcaacaggt ctttatcaat cattacgaca aaatgttttc agccgccatg aatcaaggca
     3481 ccagaatgtc atacaaaatt gattatcaaa acagggaata tggtttgttg aaaatgagcc
     3541 gtaaagtcgg cgtcagcac
//
LOCUS       DAOSME010000146         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_29662_length_3452_cov_17.985576, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000146 DAOSME010000000
VERSION     DAOSME010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29662_length_3452_cov_17.985576"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="PLG98_11525"
     CDS             <1..1534
                     /locus_tag="PLG98_11525"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOE33938.1"
                     /translation="LEAIDEELKEDYEGNKELLFKIKSINDELKKGENAPIRDIMEIR
                     DEAKRKGNLSLVENLDAILKKIKKSCKLELADGMTVRDAILNSGGLTNNYFMDRAEII
                     RVNEKKAYTTLYFNVAKAMSGDAAENLALQHKDTIVIHSIYEYEPQRIVYVDGEISRP
                     GIYQYTINMTVRDLVFKAGLLESAYLNEAEVSSQVVLGGKIVQTEYRKLNLKEALQGN
                     PAHNVVLRPHDRLMIKRITNWRETRIVEVAGEVNYEGKYVIKKGDKLSSLIERAGGYT
                     EEAYLRGAVFTREKVREMQQKSIEEMADRLEKELLSATAVAASTALNAEEVVAKKAEM
                     EQKKALIENMRKAKALGRMTIRLAHLRLLKGSEYDFELEDGDKLYIPELSSTVGVVGA
                     VMSQGAHVFSSKADYQDYIDMCGGYSSYADKSKVFILKVDGSARKAHRGVINWNEKND
                     RMEMAAFSEADTYHVEPGDVIAVPEDFARVAWLREIRDITQILMNTAVASGNLVLLFR
                     SF"
     gene            2054..2218
                     /locus_tag="PLG98_11530"
     CDS             2054..2218
                     /locus_tag="PLG98_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33939.1"
                     /translation="MQALVQVQVQVQAQVLVQAQRVLVLVQRVLLQVVLRRRALALVR
                     LQQGLPLQVS"
     gene            2674..3129
                     /locus_tag="PLG98_11535"
     CDS             2674..3129
                     /locus_tag="PLG98_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33940.1"
                     /translation="MRFGSAQNIDSALQEQVLSATENYFVQLKNKNYAAVWSSLSAKS
                     RAKIISDVRKENKKIGIESSEDSLAKDFAAGGVMAKAYWDSFLFVFNPDIVLNESKWE
                     IGKVGSDEAEIVLQYKKSEKPAVLKLYRENGSWKFGLDESFGARSLTVF"
BASE COUNT          976 a          654 c          931 g          891 t
ORIGIN      
        1 tctggaagca atagatgaag aactcaagga agattatgaa ggaaataaag agctgctgtt
       61 caaaatcaag agcatcaatg atgaactaaa aaaaggcgaa aatgctccca tcagggatat
      121 aatggaaata agagatgagg ctaagcggaa gggaaatctt tcccttgtag aaaacctgga
      181 tgccattctg aaaaaaataa aaaagagctg caaactggaa ttggcggatg gcatgacggt
      241 gagggatgct attttaaatt ccggaggcct gacgaataat tacttcatgg atagagccga
      301 aatcattcgc gtcaatgaga agaaggcata cacaacccta tattttaatg ttgccaaggc
      361 catgtccggc gatgccgcgg aaaacttagc gctgcagcac aaagatacaa tagtgattca
      421 ttcgatctac gagtatgagc cgcagagaat agtttatgtt gatggagaaa tatcccgtcc
      481 gggcatttat caatatacaa taaatatgac ggttagagat cttgttttta aagcaggcct
      541 tctggaatcc gcctatttga atgaggcgga agtttcatcg caggttgtgc tgggcggtaa
      601 gatcgttcag accgagtatc ggaaattaaa tctgaaagaa gccctccagg gtaatcccgc
      661 ccataatgtt gttctgagac ctcatgacag gctgatgatc aaacgcatca ccaattggag
      721 agaaaccagg attgtcgaag tcgcgggtga agtgaattat gagggaaagt atgtcattaa
      781 aaaaggagat aaattgtctt ctttgattga gcgggccggc ggttacacgg aagaagccta
      841 cttgcgcggg gcggtcttta cccgggaaaa agtgagggaa atgcagcaga aaagcatcga
      901 ggaaatggct gatcgtctgg aaaaagaact gctttccgcc actgccgtgg cggcctcaac
      961 ggccttgaac gctgaagaag tagtggcaaa aaaagctgaa atggaacaga aaaaagcgct
     1021 tattgagaat atgcgaaaag ctaaagcttt gggaagaatg actatcagac tggcgcattt
     1081 gcgtctgttg aaaggcagtg aatatgactt tgaactggaa gacggcgata agttgtacat
     1141 tccggagttg agcagcaccg ttggcgttgt cggcgccgtg atgagtcagg gcgcgcatgt
     1201 tttctcgtca aaggcggatt atcaggatta tatcgatatg tgcggcggct acagcagcta
     1261 cgcggataaa agcaaggttt ttattttaaa agttgacggc agcgcccgca aagctcatcg
     1321 cggcgtcatt aactggaatg agaaaaatga tcgtatggag atggctgcat tttctgaagc
     1381 ggacacttac cacgtcgaac cgggtgatgt aatcgccgtg ccggaggact ttgcacgcgt
     1441 cgcttggtta agggaaatac gtgatataac acagattttg atgaatacgg cggttgcgtc
     1501 gggtaacttg gtactgttgt tcagatcgtt ttaggataaa agatttaagg tttaagtatt
     1561 aagtttaaag tgttaagatt taagtggttg atatttaaaa ggaaattgta aaaaaggcga
     1621 tgggtgagag gttgtgggga acggtttcaa accgttcctg gggcatgggc aataggcaat
     1681 gggtgagatg cagagaacgc gtgcgagtgt tacctgcaac cgaaagtcgc aaaaacaagt
     1741 acaatcatac ttgaaaatag ctcattttag tttgtgattt ccgcttgaca aatgaaggct
     1801 ggttgaggca tatagcaatc gaaaataaat cgtatttaaa aattaaccaa aattatgaaa
     1861 ctttttattg aattgaaaaa caattgatga tggtaacttt ttcttgtgat tgaaaacata
     1921 ggaaaagaaa tttataaata atatagaaag ctggaggggt atgaaatgaa aaagctggtg
     1981 aattttgttg gaatggtggc aatgattttc tgcttggtgg catttgttcc gtcgatggca
     2041 ttttgtcagg caggtgcagg cgctggtgca ggtgcaggtg caggtgcagg cgcaggtgct
     2101 ggtgcaggcg cagcgggtgc tggtgctggt gcagcgggtg ctactgcagg tggtcttacg
     2161 gcggcgggca ttggcgctgg tacgattgca gcaggggttg ccgttgcagg tgtcgtagca
     2221 gccgttgcgg ttgcggcatc ggcctcgtcc gatacgactc cggtacacca cggaacgaca
     2281 tctcatcact aacagactct tggtttgaaa agatttcaag aggcgaacac aggtcgcacg
     2341 aagcgatctg tgttttcgcc tttttttata acaggctgca ttaggattgt ttcgaattcc
     2401 tttttatgga ggagactgaa cgctttctga cgcttgttct gccaatgttt tctggagtcg
     2461 agggcgctaa attttccgat atcgcctaac caattttatc gaatgaaaac tttgccaaac
     2521 cacataaacc caataaattt gtcattccgg cgcaggccgg aatccagaga attcttttca
     2581 atcaacaatt cggttttcag ccgctgtgac aaaggcaggg ctttagcatg taccactgcg
     2641 ctggttctga tcgtatttgt tttgttctcc cccgtgcgtt tcgggtcagc ccaaaatatc
     2701 gattcagcgc tacaggaaca agttttatcg gctactgaaa attattttgt tcaactgaag
     2761 aataaaaatt atgcggcggt ctggagttcg ctctccgcta aatcgcgggc gaaaattatc
     2821 agcgacgtgc gcaaggaaaa taaaaaaatc ggcatcgaaa gcagtgaaga ctcactggca
     2881 aaagattttg cagccggcgg ggtgatggcg aaggcttatt gggacagttt tctgtttgta
     2941 tttaatcccg atattgttct gaatgaatcc aaatgggaaa ttggaaaagt tggttctgac
     3001 gaggcggaaa ttgtcctgca atacaagaaa tccgaaaagc cggccgtcct gaagctgtat
     3061 cgggaaaacg gttcatggaa gttcgggctg gacgaaagct ttggcgcccg gagtttgact
     3121 gtgttttgag ggcttttata ttgtcatcct gagccgctct cttgtcatcc tgagcccgtc
     3181 gaaggatgac gcctctttat ttctattatg gttcgactgg ctcaccactc gaccagctca
     3241 gcatgacaag ttgggctcac catgacaaga taaatgtagg gcgtgccgct tgtgaccttc
     3301 aggttatcag gcgcgccaac atatccgtag ggaatatttg tagggaacgc tcgcgagcgt
     3361 tcctcgtgta acgcatttgc aaggaacggt ctcgaccacc cctgcgggtg gcaagccgtt
     3421 ccctacacat tgcttttttg gcttacgatg cg
//
LOCUS       DAOSME010000147         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_29990_length_3423_cov_23.255344, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000147 DAOSME010000000
VERSION     DAOSME010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29990_length_3423_cov_23.255344"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3423)
                     /locus_tag="PLG98_11540"
     CDS             complement(<1..>3423)
                     /locus_tag="PLG98_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33941.1"
                     /translation="NMSMNASTIGLVVAAIAALTTVIVPFNRKAKETNAEMEAIVSAT
                     KKATEKFAEEKAKIEALNTILHDTNQSYKERKRALDEIQQLAPDYHAKLTTEGELIND
                     NTQSIEDHVKSLEKDIQAEMLRAEIVEQIRQREMEMETARERIARGYQVLVSAGASGA
                     GFQVQNWVTQAYTEAEAQEILTEAYNNRQSAIDALTKKLAELFSSDSKTAQKSKQLSK
                     EELEELKKKKEQKQKIQEEYDKWLLDATNAANDELVNLQLQAERDRVDAMAEGASKRK
                     AQFSLEQKKAIIEMQRYVRNVLNEQAKLEKNKGMSIGYGTFEVGADGTLAYKLAESGL
                     TLTPEQQNVINEMVRKFNQDIVDTWVTEVTDLDIDSGYNLADKLLEQLDEEMISGLEK
                     GGFNPLKQQASLIFADISDLSAEMIQSIIANAEQYLASNDLPEDIVSNFRGYIDKANE
                     YLVEKNPWEALDIAQERYKKAQEDLKNAMATGDFEKQQQAILEMSNALSIAGKALDEI
                     NGIYDDMLGLLGEVAQAMGMDSGLASSIQNLVSAASKFAGEAVKSLGSIEKIGDAAQD
                     ATKKIGTGAATGASMGGSMGGLIGAFIGLAIAIVKVSSAIAEAEDAASEEQISRYIDK
                     INAKVDSLKRNLEAIASASGPGDGWFTEDFFNNVQRANDVVVQAMKDITGTLDDTYKR
                     WNEVFQRMYGRSIYNKGGGENIKNLLDLIKQIRSTLGYKDVDYDLIPDYLRNGESSKA
                     LEFSIDNLTKYVNLLKDLEAEYTTDGADVLAAEARALYEGLESYINAYKSLQESIVAL
                     TGDISGNILSTVNSMWKEIREGGETTFADLTAAAKKNISEVIDQMVSQQLWATTMSKY
                     FDQLGVDLTAAIAGGGDPSAILGVFDTFWTGMEAGLKDYTKYYEAFLQKAEERGWDMT
                     SGNKKEENPAAGSIKAMRDELSKLQEQWDSLSAAERESAEGEKLYGDIGNLEKRIQAA
                     EDLYNVNIDVADSINGINAELSRLEAEWNAMSKAEREGAKGMKNRADKTYYEGLLNQA
                     QGVQTSELEKMQALLDSAEAKYSLYQKWVQLYGKDTADQMMGDMLDDAESYVDELRSG
                     IAELTAKVTEGTATDEEIAQLEAWEGQLEDIMNQGAQAAITAANEASQAWNDS"
BASE COUNT          727 a          927 c          685 g         1084 t
ORIGIN      
        1 gaatcattcc aagcctgtga tgcttcgttt gctgcggtta tagcagcttg tgcgccctga
       61 ttcattatgt cctccaactg accttcccaa gcctccagtt gtgctatttc ctcgtctgtg
      121 gccgttcctt cggttacctt tgccgtcaat tcagcaattc cggaacgcag ctcatccaca
      181 tagctttcag catcatccag catatcaccc atcatctggt ctgccgtatc ttttccgtat
      241 agttgtaccc atttctgata aagcgagtat ttcgcctctg cggaatccaa aagggcttgc
      301 attttttcaa gttcggaggt ctgtacgcct tgtgcctgat tcaatagacc ttcataatat
      361 gtcttgtctg ccctattctt catccccttc gcgccttccc gctctgcttt gctcattgcg
      421 ttccactcgg cctccaagcg gctcaactcg gcatttattc cgtttattga atcggcaaca
      481 tcaatgttta cattgtacaa gtcctcggct gcttgtatcc tcttttccaa attcccaata
      541 tccccgtaca gcttctctcc ctcggcactc tcacgctctg cggcacttaa actatcccat
      601 tgctcctgca attttgacaa ttcgtcacgc attgccttaa tggaaccggc agcagggttt
      661 tcttccttct tattaccaga ggtcatatcc caaccacgtt cttccgcttt ttgtaggaac
      721 gcctcatagt atttagtgta atcctttaat ccggcttcca ttccggtcca gaaagtgtca
      781 aacaccccta aaatggcaga tggatcacca ccaccggcaa tggcggcggt aagatcaacg
      841 cctaactggt caaagtattt tgacatggtg gtggcccaaa gctgctggga taccatctgg
      901 tcgattacct ctgaaatgtt cttctttgcg gctgccgtaa ggtctgcgaa agtcgtttct
      961 ccaccctccc ggatttcctt ccacatggaa tttaccgtgg aaaggatatt gccggaaatg
     1021 tctcccgtta gcgcaacaat ggattcctga agggatttat aggcgtttat atagctttca
     1081 agtccttcat aaagagccct cgcctcggca gcaagcacat cagctccatc tgtggtatat
     1141 tcggcctcca ggtctttcag caagttgacg tatttggtaa ggttgtcgat actgaattcc
     1201 aacgcttttg acgattcccc gtttctcagg taatccggga tcaggtcata atccacatcc
     1261 ttatacccta acgtactgcg aatctgttta atcagatcga gcaggttttt tatgttctcc
     1321 ccaccgcctt tgttgtaaat ggaacggccg tacattctct ggaaaacctc attccacctt
     1381 ttataggtgt catcaagcgt tccggttatg tctttcatag cctgaaccac tacatcatta
     1441 gccctttgca cgttgttgaa gaaatcttcg gtaaaccagc cgtcacccgg gccggatgcc
     1501 gatgcaatgg cctccaggtt tcttttcagg ctgtccacct tggcattgat cttatcgata
     1561 taccttgata tctgttcctc cgaggcggca tcctcggcct cggcaatggc acttgaaacc
     1621 ttaacgatag caatagccaa cccgataaaa gcgcctatga gaccacccat tgaaccaccc
     1681 attgatgcac ccgtggccgc ccctgtccct atctttttcg ttgcgtcttg tgctgcatct
     1741 cctatctttt caatcgaacc taaggacttc accgcttcac cggcaaattt ggaagctgcg
     1801 cttacaagat tttgaataga gcttgccaat ccgctatcca tacccatggc ctgcgccacc
     1861 tctcccaata aaccaagcat atcgtcatat atgccattta tttcatcaag agcctttcct
     1921 gcaatggaca aagcattgga catctcgaga atggcttgtt gctgtttctc aaaatcaccc
     1981 gtagccattg cgttcttcaa atcctcttgc gcctttttgt atctctcctg agcaatgtct
     2041 aaagcctccc atggattctt ttctaccaaa tactcattgg ctttgtctat gtatcccctg
     2101 aaatttgaaa ctatatcttc cggtaagtca ttggaagcaa ggtattgttc ggcattagcg
     2161 attatgctct gtatcatctc ggccgagagg tctgatatat cggcaaaaat cagtgaggct
     2221 tgctgtttca aggggttaaa gcctcccttt tcaagcccgg aaatcatttc ttcatccaac
     2281 tgctccagga gtttgtcggc cagattgtat ccggaatcaa tgtcaagatc ggtaacctca
     2341 gtaacccacg tgtcaacgat gtcctgattg aacttacgaa ccatctcatt tattacgttc
     2401 tgctgttcag gggttaaggt taatccgctt tctgccagct tatatgcaag ggttccatcg
     2461 gccccgactt caaaggttcc gtatccaatg gacattcctt tgttcttttc cagcttcgct
     2521 tgttcattga gcacattcct tacatatcgc tgcatctcta taatggcttt cttttgttcc
     2581 aaagagaact gagccttgcg cttggaggct ccttcggcca tggcatcaac ccggtctctc
     2641 tcggcctgaa gctgtagatt taccagttca tcgttggcgg cattggtggc atcaagaagc
     2701 cacttgtcat actcttcctg tattttctgt ttctgctcct ttttcttttt gagttcttcc
     2761 aactcttcct tggaaagttg tttgcttttc tgggctgttt tgctgtcact ggaaaacagt
     2821 tcggcaagtt tctttgtcaa ggcgtcaatg gcagactgtc ggttgttgta agcttcggta
     2881 agaatctcct gagcctcggc ttccgtatag gcttgtgtta cccaattctg aacctggaat
     2941 cctgccccgg aagctccggc agataccaaa acctgatacc cccttgctat acgttctctt
     3001 gcagtttcca tctccatctc cctctgccgt atctgctcga caatctcagc ccgtagcatt
     3061 tcggcctgta tatctttttc aagagactta acgtggtctt ctattgattg agtgttatca
     3121 tttatcagct cgccctcggt tgtgagtttg gcgtgataat ccggagcaag ctgctgtatt
     3181 tcatcaagag cccttttgcg ttctttatac gattggttgg tgtcgtgaag aatggtgtta
     3241 agagcttcta tctttgcctt ttcttcagcg aatttctccg tagctttctt tgttgccgag
     3301 acaatggctt ccatctcggc attggtctct ttagccttgc gattgaacgg tacaataacc
     3361 gttgtcaatg cggcaatagc ggccactaca aggccgattg tgcttgcgtt catcgacata
     3421 ttc
//
LOCUS       DAOSME010000148         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_30036_length_3419_cov_19.059453, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000148 DAOSME010000000
VERSION     DAOSME010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30036_length_3419_cov_19.059453"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2962)
                     /locus_tag="PLG98_11545"
     CDS             complement(<1..2962)
                     /locus_tag="PLG98_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOE33942.1"
                     /translation="MKRKSLKMIGAGFLAAVMFFAAGASVCFAQQTKTSDRSEKPEIF
                     IQLGHSGNIRSVAFTSDGTYLVSGSDDRTVKIWEAATGKEIRTLRHDEEVKAIAISPD
                     NKTILAGDRQGNITLWETATGKSLGKLSAKGLGELASLSFSPDGRHAVATDFFKLVYF
                     DVMAKKEIFALDRGQDKFTKDLAAQVNVAAFTKDSRYLVLGIKKTDSYYNSQGISIGI
                     YDIQRRKRIKNFDLLKGNTEIYSLAISPDEKFFLVSASLARSKDMFEKSGKVFVVDAQ
                     NGGIIKEFYFPEPFFEVAYSPDGHHAAIGDHGEIIIYETNHWNVARKIRKHLPLAFSP
                     DGQSLIYGNDNTWAYYGQWVHKTGLDLMDLKTGNSVNRYAANVDQVTEASFAANEREI
                     MSRSWILLNWDRQNGTPKKPLILKDSSNRMITANAASPDGKYLIVGNDYGSYIYHTSD
                     GRMRRMWEKPLFRPDFSEDGKTLFFSTYDRKIILWDIENNRQLRQFADWGRREQLYIN
                     FNKISPDGRYAVVLFEDHEQQGSKAIMIVWDANTGKEIRRQEIKNSIFSLVLSPDSRH
                     ILTGESQYGVQAAKDKHVTVLRSITDNRIVKKFPGHTGIISALAFSRNGEFILTGSWD
                     QQIILWEVKSGKRIKTFTGHAGIIYTLEFSSDNKSFVSTSDDNTVRLWDIPAGRESAK
                     FISFVDGEWIVITPEGYFNASQGGAKHLSVRLGNRVFPIDNLYEKFFDPVYVASVLQG
                     KKVEATADIRKGIQPPPDIRILSPAPDAVMATDTVTVQVGARDTGGGIDEIRLYHNGK
                     ALGDDRRGVKLVSRGGETVRDFQVTLVDGANTFRAVGFSSDRTESNPAELTIQLSAPS
                     KEVSLYVFAVGINQYRNPALNLNYAVPDAKGIAGFFRQKGQLFKKVNIIEIYDEEATK
                     DGILAKLRELEKTNPQDAVLIYLAGHGENVKDIWYFIPHELTYPEREEHVQTKGISST
                     EFSEAMKKIRAQKIL"
BASE COUNT          763 a          847 c          873 g          936 t
ORIGIN      
        1 tcaggatttt ttgcgcccga atcttcttca tggcttccga aaattccgtg gaagaaatgc
       61 ccttggtctg aacatgttcc tcgcgttccg gataggtcag ctcgtggggg atgaaatacc
      121 agatgtcctt gacgttctcg ccgtggccgg ccagatagat cagcacggcg tcctgcggat
      181 tggttttctc cagttcgcgc agtttggcga ggattccgtc tttcgtcgct tcttcatcgt
      241 aaatttcgat gatgttcact ttcttgaaga gctgaccctt ctgccggaag aatccggcaa
      301 tgcctttggc gtccggcacg gcatagttga ggttcagcgc gggattgcgg tattggttga
      361 tcccgacggc aaaaacataa agagacactt ctttggaagg cgcggaaagt tgaatggtta
      421 attccgcggg attggattcg gtgcggtcgg agctgaagcc cacggcgcgg aaggtgttgg
      481 ccccgtccac cagcgtcacc tgaaaatccc ggacggtttc gccgccgcgg ctgacgagtt
      541 tcactccccg ccggtcgtcg cccagcgcct tgccgttgtg ataaagccga atttcgtcga
      601 tgccgccgcc cgtgtccctg gcaccgactt gaacggtgac ggtgtcggtg gccatgacgg
      661 cgtcgggcgc gggcgaaaga atccggatat ccggcggcgg ttgaatgccc ttgcggatgt
      721 cggctgtcgc ctccactttt ttcccctgca ggacggaagc gacgtaaacg ggatcgaaga
      781 atttttcata cagattgtcg atggggaaaa ctctgttgcc caagcggacg gagaggtgct
      841 tcgctccgcc ctgcgaggcg ttaaaatagc cttccggggt gatgacaatc cattcgccgt
      901 ccacaaaaga gatgaatttt gcgctttccc tcccggcggg aatatcccag agacggactg
      961 tattgtcgtc gctggttgat acaaaactct tattgtccga agaaaattca agcgtgtaga
     1021 taattcccgc gtggccggta aaggttttga ttcttttgcc gcttttaact tcccacagaa
     1081 tgatttgctg atcccagctt ccggtgagaa tgaattcacc gttgcgggaa aaggccagcg
     1141 cgctgattat acccgtgtgt cccgggaatt ttttaacgat ccggttgtcc gttatggaac
     1201 gcagaacagt aacgtgttta tccttggctg cctggacgcc gtattgtgac tcacctgtca
     1261 gaatatgacg ggaatcaggt gaaagaacca gagaaaagat gctgtttttg atctcctgcc
     1321 gtctgatctc ttttccggta ttggcatccc agacaatcat gatggctttc gatccttgct
     1381 gttcgtggtc ctcaaacaaa acaacggcgt aacggccgtc cggggatatt ttattgaaat
     1441 tgatatagag ttgttccctg cggccccagt ccgcaaactg tcgcagctgc cggttgtttt
     1501 caatatccca caaaatgatt tttcggtcat atgtggaaaa gaaaagcgtt ttgccgtcct
     1561 ctgaaaaatc cggccggaaa agtggttttt cccacatcct gcgcattcgg ccgtcggagg
     1621 tgtgatagat atagcttccg taatcgttgc cgacgatcag gtatttaccg tccggacttg
     1681 cggcgtttgc ggtaatcatt cggtttgacg agtctttcag aatgagaggc ttcttcggcg
     1741 ttccattctg tctgtcccag ttcagcagaa tccagcttcg ggacatgatt tccctctcgt
     1801 ttgccgcaaa agaagcttcc gtcacctggt caacattcgc tgcgtaacga ttgacggaat
     1861 ttcctgtttt gaggtccatt aaatccagtc cggttttgtg aacccactgg ccataatatg
     1921 cccacgtatt gtcattgccg taaatcagag attgtccgtc cggcgagaaa gccaggggca
     1981 ggtgtttccg gattttcctg gcgacattcc agtggttcgt ttcataaatg ataatctctc
     2041 cgtgatcgcc gatcgccgca tgatgaccgt cgggggaata ggcgacttca aaaaacggct
     2101 cgggaaaata aaactccttg atgatgccgc cgttttgagc gtcaacgaca aacacttttc
     2161 cggatttttc gaacatatcc tttgatcgag caagtgatgc ggaaaccagg aagaactttt
     2221 cgtcgggaga aatcgcaagg gaataaattt cggtgttacc ctttaaaagg tcaaagtttt
     2281 ttatgcgttt cctcctttga atgtcgtaaa tcccgatact gatgccctga ctattatagt
     2341 agctgtcagt ttttttgata cccaaaacca ggtatcttga atcttttgta aaggcggcaa
     2401 cattgacctg agcggctaaa tctttggtaa atttgtcctg ccctctgtcc agggcaaaaa
     2461 tttccttttt cgccatcaca tcaaagtaaa caagcttgaa aaagtccgtg gcaacggcat
     2521 gcctgccatc cggtgaaaaa gaaagcgatg caagctcacc caagccttta gccgacagtt
     2581 tccccaatga ttttccggtc gctgtttccc agagggtgat attcccctgc cggtcaccgg
     2641 caagaatcgt tttattgtcg ggagagattg cgatcgcctt gacctcctcg tcatgacgca
     2701 gcgtcctgat ttccttgcct gtggccgctt cccagatttt gaccgtccgg tcatcactgc
     2761 cggaaaccag ataggtgccg tcggaagtga aggcaacgga tcggatgttg ccggaatgcc
     2821 ccaactggat gaagatttct ggtttctcgg agcggtctga cgttttcgtt tgttgggcaa
     2881 aacagacgga tgctccggcg gcaaaaaaca ttacagccgc aaggaatccc gcgccgatca
     2941 ttttcaatga ttttcttttc atagtgcttc tcccgatttg ccggctgtct gtacggtaca
     3001 agccgtcatt aagcgccttt gtttcttttt aatcaaaccc ggtccatttt gcaaggccgg
     3061 tttccggtgt tgtcaaattt tggttaaaag gagagaaatt gatatgttgg attctatcag
     3121 ccgtgggatt tttttaatct ctacgggtca atttcccgaa gcttgcttcg ttcctttggg
     3181 tttggatacc ccgtagcttg ctgcggggta gttcattatc ccgtattcct cttagggaac
     3241 ggtttcaaac cgtttcctaa gcctcatcac ctatcgcctt gatccataat ttctcgtccg
     3301 aaaaaacttc gtggcaattc ttaccgggat cggataccat gcaggcggcc gccggctaat
     3361 tcaatgaagg aaaggaggca acgatgaagc ttaaaacaaa agacggagga tggaatcct
//
LOCUS       DAOSME010000149         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_30439_length_3384_cov_17.159507, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000149 DAOSME010000000
VERSION     DAOSME010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30439_length_3384_cov_17.159507"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLG98_11550"
     CDS             complement(<1..747)
                     /locus_tag="PLG98_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOE33943.1"
                     /translation="MAQTGNCRNKNGNDQDTAWDATALRPFAFIGRTVLGWVGNLGAA
                     AIFLGMALWQIFRRKQFAKTIRQIYYIGARSITIIMLVSLFTGMVLGLQAHHALSKFG
                     AEGAVGSLVALSLIMELGPVLTAIMITARAGSAIAAEIGIERISEQIDALQTMRINPL
                     RYIVSPKISASIISFPLLTAVFDLIGILGGYLSAVLLLGLNSGVYWHSTKVYVALEDV
                     TNGFIKAIVFAVIVATVCCYQGYFAHMRRDS"
     gene            932..1468
                     /locus_tag="PLG98_11555"
     CDS             932..1468
                     /locus_tag="PLG98_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33944.1"
                     /translation="MIRYFNATHIPVAARAFSDAEKLVSRHFRLTEDDLRKNKYDVKT
                     LAFLEQHEVNDGAFAHLCKYSYEKSSDSKPEEKEGFEFYRVCLQDHIILDALERANTF
                     IKLSPLMLYIAVHELIHVLRFSTGAADFSAPQEEKEQEEKIVHNLTRSALQPARQTDM
                     NMVLDCFSSEYKISNLYN"
     gene            1486..1764
                     /locus_tag="PLG98_11560"
     CDS             1486..1764
                     /locus_tag="PLG98_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE33945.1"
                     /translation="MPIYEYQCRKCGRQFEAFQGITEPDLKTCRFCRGKVQKMMSLSS
                     FSLKGTGWYATDYKGKKPQTVKPKSTETAESSPVSSDASATVKGVSDD"
     gene            complement(1873..>2083)
                     /locus_tag="PLG98_11565"
     CDS             complement(1873..>2083)
                     /locus_tag="PLG98_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33946.1"
                     /translation="NNFMSFNLSLYAPMLPEKNFWVQGKIIPRFEINPLGTSDLNYFE
                     KLHNEIICPIVPEQLCKELQAFFQE"
     assembly_gap    2084..2169
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2170..2825)
                     /locus_tag="PLG98_11570"
     CDS             complement(<2170..2825)
                     /locus_tag="PLG98_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33947.1"
                     /translation="MKDIGFHRETVTGRYLNPFDGFEPREMTIEERYDETTGVASRIL
                     PYRVRPAQKPDTGVYLEKSPESICPFCPELFDKMTPRFTPDIIEGGKFTRGSAFLFPN
                     AFPYDATNTVAIFSHRHFMGLDELTPDAMHDGFAVCRDYFYRMAELQQGYHYGSINWN
                     YMPPAGGGLIHPHLQTIAGKKPTNFMRRLLASAQNYEAASGGGNLWKDLLDREQDAGE
                     R"
     gene            complement(2822..>3384)
                     /locus_tag="PLG98_11575"
     CDS             complement(2822..>3384)
                     /locus_tag="PLG98_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33948.1"
                     /translation="GIKTDTDNFDRETLAEDIRAYTYHSRHANLRLIRRIELNQTPHS
                     YLKYFDYAFHSMTHFRGRRVGFLGRVESPDVCVQVADFYLHVIGTSYVVVAGIVDDRL
                     VIIFRGDGYRQDCGAMAQQACGQIGQGGGHRSAARLEIPLDVLKTELKEDLSRQSVER
                     FLHQCLRKSHLPKEDPPAAGSGREKT"
BASE COUNT          791 a          890 c          871 g          746 t
ORIGIN      
        1 gctgtcccgg cgcatgtgcg cgaaataccc ctgatagcag caaaccgtcg cgacgatcac
       61 cgcaaaaaca atcgctttga taaaaccgtt ggtgacgtct tccagagcca cataaacctt
      121 cgtgctgtgc cagtaaacgc ccgaattcag ccccagcagc agaaccgcgg aaagatagcc
      181 gcccagaatg ccgatcagat cgaatacggc cgtgagcagc ggaaagctga tgatggaggc
      241 ggaaatcttt ggactgacga tgtaccgcag cggattgatc cgcatcgtct gcagggcgtc
      301 aatctgttcg ctgatgcgct ctatgccgat ttcggcggca atggccgaac ccgcccgcgc
      361 cgtaatcatg atggcggtaa gcaccggacc gagctccata atcagcgaaa gggccaccag
      421 agacccgacc gccccctccg cgccgaattt cgacagggcg tggtgggcct gaaggcccag
      481 caccatcccg gtaaacaggc tgaccagcat gatgatcgtg atggatctgg ccccgatata
      541 ataaatctgc cggattgtct tggcaaactg cttcctccgg aaaatctgcc acagcgccat
      601 cccgagaaaa atggccgccg ccccgaggtt acccacccag cccagaacgg ttcttccgat
      661 aaaagcaaaa ggacgaagcg cggttgcatc ccaggcggtg tcctggtcgt ttccattttt
      721 attccggcag tttccggttt gtgccatggg tatgtttcct tgatgaaaat cgccttttga
      781 aggcggtttg ggatactaac atcggcaatc ttatttgaca agcatttcct aacacctgtt
      841 tcccgtccgt atgtttcccg tccgtattga cagaaacacg gagaatgaat atactaccgt
      901 caaatcgggc agcggcattt aaggtgtttt tatgattcgc tattttaacg cgacgcatat
      961 accggtggcg gccagggcgt tttccgacgc tgaaaagctc gtgtcgcgtc atttccgtct
     1021 gacggaagat gacctgcgga aaaacaaata cgatgtaaaa acccttgctt ttttggaaca
     1081 gcatgaagta aatgacggcg catttgccca cttgtgcaaa tattcctacg aaaagtcttc
     1141 cgacagcaag ccggaagaga aggaaggttt cgagttttac cgggtatgcc tgcaggacca
     1201 tatcatcctg gacgcgctgg agcgagccaa tacgtttatc aaattgagcc ctttgatgct
     1261 gtatattgcc gtccatgagt tgattcatgt gctgcgcttc tccacggggg ccgctgattt
     1321 cagcgctcct caggaagaaa aggaacagga agaaaaaatc gtccacaacc tgacccgaag
     1381 cgcgctgcag ccggcaaggc aaacggatat gaacatggtt ttggactgct tcagcagcga
     1441 atataaaatc agcaatcttt ataattaatt tcaggaggtt gatgtatgcc gatctatgaa
     1501 tatcaatgca ggaaatgcgg ccggcaattt gaagcgtttc agggcattac cgagccggat
     1561 ttgaaaacct gccggttctg caggggaaag gttcagaaaa tgatgtcgct ttcatccttc
     1621 agtctgaagg gcaccggctg gtacgcgacg gactacaagg ggaaaaaacc tcagaccgtc
     1681 aaaccgaaaa gcacagaaac ggcagaaagt tccccggttt cttccgatgc ttcagcgacc
     1741 gtcaaaggcg tcagcgacga ttaaaagcat tttatcaaaa agaccaaata aaaaagcggc
     1801 atcggaatgc cgctttttta ttgaccacac tggagcaata aaacgcagag cgttctaatc
     1861 ccactgtcaa atttattcct gaaaaaacgc ctgtaattct ttgcacaact gctccggcac
     1921 aatcggacag atgatctcat tgtgcagttt ttcaaaataa ttcaaatcgc tcgtacccag
     1981 gggattgatt tcaaaacgcg gaatgatttt tccctgcacc cagaagtttt tctccggcag
     2041 catcggcgcg tacagggaaa gattgaagct catgaaatta ttgnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnc gctcgcctgc gtcctgctca cgatccagca ggtctttcca aagattgccg
     2221 ccgccggagg cagcttcgta attttgtgcg ctggccagca gccgccgcat gaaattggtc
     2281 ggttttttgc ccgcgatcgt ctgcaggtgg ggatgaatca gtccgccgcc cgcgggcggc
     2341 atgtagttcc agttgatcga accgtaatgg tacccttgct gcagttcggc catccggtaa
     2401 aaataatccc ggcagacggc aaatccgtcg tgcatggcgt cgggggtcaa ttcatccagc
     2461 cccatgaagt gtcggtgcga aaagatcgca accgtgtttg tcgcgtcgta gggaaacgcg
     2521 ttgggaaaca gaaaagcgct tcctctcgtg aatttgccgc cttcgatgat gtccggcgtg
     2581 aaccggggcg tcatcttgtc aaagagctcc gggcaaaacg ggcagatcga ttccggcgat
     2641 ttttccagat agacgcctgt gtccggtttc tgggcgggcc gcacacggta aggaagaatg
     2701 cggctggcca cgccggtggt ttcgtcgtag cgctcctcaa tggtcatttc cctgggttca
     2761 aacccgtcaa agggattgag gtaccgtccg gtgaccgttt ccctgtgaaa tccgatgtct
     2821 ttcatgtttt ttccctccca cttccggccg caggcggatc ttctttcggc aaatggcttt
     2881 tcctcaggca ttgatgaaga aaacgctcca cgctctgccg ggacaaatcc tctttcagct
     2941 ccgttttgag aacgtccagg gggatttcca ggcgcgcggc gctgcggtgt ccgcctccct
     3001 gtccgatctg gccgcaggct tgctgcgcca tcgcgccgca gtcctgccgg tagccgtccc
     3061 cccggaaaat aatgaccagc cggtcatcca cgattcccgc aaccaccacg tacgacgtgc
     3121 cgatcacgtg caggtagaaa tccgccacct gcacgcagac atccggactt tccacgcgtc
     3181 ccaggaaacc gacgcggcgg ccgcggaaat gcgtcatgga atgaaacgcg taatcgaaat
     3241 atttcaaata gctgtgcggc gtctgattga gttcgatccg gcggatcagc cgcaggttgg
     3301 cgtgccggga atgataggtg taggcgcgaa tgtcttcggc cagcgtttcg cggtcgaaat
     3361 tatccgtgtc cgtcttgatg ccgt
//
LOCUS       DAOSME010000150         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_30616_length_3368_cov_24.858436, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000150 DAOSME010000000
VERSION     DAOSME010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30616_length_3368_cov_24.858436"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PLG98_11580"
     CDS             complement(<1..1577)
                     /locus_tag="PLG98_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOE33949.1"
                     /translation="MNAFQTVAEFKAYRKSIIENHKKHPAKPCLVICGGTGGQASGSN
                     DLIRIIKRRILEQNLQDKLALRITGCLGFCEMDPFIIVEPGYNLYPKLKMVDVSRIVD
                     AAVKGEVVEELLYREPGSTVKRFTEQDIPFFQGQTRIVLGQNQHLDPIRISDYLKTGG
                     YEALEKALEKPDGNWIVEEILKSRLRGRGGAGFLTGKKWELARNVNKGHTPKYIICNA
                     DEGDPGAYMDRSMLEGNPHAVIEGMIIASIAIGANNGIIYVRTEYPLAIKHASIALRE
                     ARRLDLLGKNILGTGIDFDIEIFQGAGAFVCGEETALIKSIEGKMGEPRQRPPFPIQK
                     GLFGHPTCINNVETLSNIPYIIKHGADEYLKIGTANNSGTKIFSLVGKIKNTGLVEVP
                     LGTPISTVVYDIGGGPSGSAKIKAIQTGGPSGGCIPSSMFDLPIDYDSLAKVGSIMGS
                     GGMIVMDQNTCMVDVAKYFMNFLKNESCGKCFTCRKGTQRLYEILEDITLGKGTPQHL
                     ILLEELAQTVKDTTMCGL"
     gene            complement(1574..2452)
                     /locus_tag="PLG98_11585"
     CDS             complement(1574..2452)
                     /locus_tag="PLG98_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE33950.1"
                     /translation="MDTKNILDIIKKHNKNKVSLISILEEIQGAYTYLPEKALRLLAK
                     ETGCSLTDIYGVATFYKAFSLKPKGKHCVSVCLGTACHVRGAQNIVEEFKRELSISPG
                     ETTPDNEITLETVNCLGACALGPTVVSDGQYFPHVSKSSVKEIITRTKGGGRSIAAEA
                     DIRIFSLQVSCPLCGKSLMDHDYIINGHPSVKFNVSMNGKKVWLRMSSLYGKHTVELE
                     NQLPPNELSRFHCPHCHNEFPSFSTCPECGASMAALFVREKCSWEVCTRQGCQGHILN
                     LDQINLHKKSNKGKNK"
     gene            complement(3034..>3368)
                     /locus_tag="PLG98_11590"
     CDS             complement(3034..>3368)
                     /locus_tag="PLG98_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE33951.1"
                     /translation="VQALMGYAWPGNIRELENLIQRLIIMTDGQKIEITDLPSVMRFS
                     ITPGSVPFRPLAEEEAAYIRRVLASVGGNKSRAAEILRIDRKTLRDKLKPNDSEINIA
                     TKKNNKSV"
BASE COUNT          787 a          791 c          821 g          969 t
ORIGIN      
        1 agaccgcaca tggtggtgtc cttaacggtc tgtgctaact cctccagaag aatcagatgt
       61 tggggcgttc ccttgccgag tgtgatgtct tccagaattt catacagcct ctgcgttcct
      121 ttgcggcagg tgaagcattt cccgcaggac tcgtttttaa gaaagttcat aaaatatttg
      181 gccacgtcca ccatgcaggt gttctggtcc atgacaatca tgccgcccga ccccatgatg
      241 gagccgacct tggccaggct gtcgtagtct atgggcaagt cgaacattga agaaggtatg
      301 cagccgccag atgggccacc ggtctgtatg gctttaattt tagctgatcc cgaggggcca
      361 ccgccgatat cataaaccac cgtactaatc ggtgtgccga gcgggacctc aaccagtccg
      421 gtatttttta tcttgccgac cagggagaaa atcttggttc cggaattatt tgccgtgcca
      481 atcttcagat actcgtcggc gccgtgtttg atgatgtagg gaatgttcga cagtgtttcg
      541 acgttgttga tgcaggttgg gtgaccgaaa agtccttttt gaatcggaaa gggaggacgc
      601 tgacgaggtt cacccatctt gccttcgatg gatttgatca atgccgtttc ctctccgcag
      661 acgaatgcac cggctccttg aaaaatttca atatcgaaat caatgcccgt acctaaaatg
      721 tttttcccca gaagatccaa acggcgggct tctctcaggg cgatggaggc atgcttgata
      781 gccagaggat attccgtgcg cacataaatg atgccattat tggctccgat ggcgatagac
      841 gcgatgatca tgccttcgat gaccgcatga ggattgcctt cgagcatgct gcggtccatg
      901 tacgcgcctg gatccccctc atccgcattg catattatgt acttcggcgt atgcccctta
      961 tttacattcc gcgccagttc ccattttttc ccggttagaa accccgcacc tcctcgtccg
     1021 cgcagcctgg atttcaggat ttcttccacg atccaattac cgtccggttt ttccagcgct
     1081 ttttctagtg cctcataacc gcccgttttc aaataatcgc tgatccggat ggggtccaga
     1141 tgctggtttt ggccgagaac aatccgggtt tgcccttgaa agaagggaat atcctgttct
     1201 gtaaagcgtt tgaccgtcga acccggctct cggtatagga gttcttcaac tacctcgcct
     1261 tttacggctg cgtcgactat tctggacacg tctaccatct tcaacttcgg gtacagatta
     1321 tatcccggtt cgacgatgat gaacgggtcc atttcgcaga agcccaggca acctgtgatg
     1381 cggagagcaa gtttgtcttg tagattttgc tccagaatcc gacgtttgat gattcgtatt
     1441 aaatcgttcg agccactggc ctggccgcct gtcccgccgc aaataacgag gcagggtttg
     1501 gccggatgtt ttttatgatt ctcgataatg gatttgcggt acgccttgaa ttcagcgacg
     1561 gtttgaaaag cattcattta tttttccctt tgttggattt tttatgcaga ttgatctgat
     1621 ccaggttcaa tatgtgcccc tgacaccctt ggcgcgtaca cacttcccat gagcactttt
     1681 cgcggacaaa cagggcggcc atggaagcgc cgcattccgg gcaggtggaa aaactcggga
     1741 attcattatg gcaatgcgga caatgaaagc gggatagctc gtttggcggg agctgatttt
     1801 ccaactcaac ggtgtgtttg ccgtaaaggg atgacatgcg cagccacact tttttaccgt
     1861 tcatcgaaac attgaattta acggacggat ggccgttgat aatataatcg tgatccataa
     1921 ggctttttcc gcagagggga caggaaacct gcaatgaaaa tatacggatg tccgcttcag
     1981 cagcgatgct tctcccgccg cctttggttc tagttatgat ttcttttacg ctgcttttac
     2041 tgacatgtgg aaaatattgg ccatcagaca caacagtcgg tcccagagcg caggctccga
     2101 ggcaattgac cgtttctaaa gttatttcat tgtcgggcgt ggtttcgccc ggagatatgc
     2161 tgagttcacg tttgaattcc tcgacgatgt tttgtgcgcc acggacatgg catgccgtgc
     2221 ccagacatac agaaacgcaa tgtttgcctt tgggtttcaa actgaaggct ttgtagaagg
     2281 tggcgacgcc gtaaatatcc gtcaatgaac atccggtttc tttggccaac agccgcaaag
     2341 ccttttcggg taggtatgta taggctcctt ggatttcttc cagtatggaa ataagcgaga
     2401 ccttgttttt gttgtgcttt tttataatat caagaatgtt ttttgtatcc atgtatatcc
     2461 tttcctcgat tgaacagatc ttcttttgtt tcgcgaaatg tcttacctgt attcctcgca
     2521 atgccataca cccgactctg gtttgggaaa gctacataca ggatattgag cacaattgct
     2581 gcacaggcct agaattttgg aaggtgcctg tttaaatctt tcggaaggct gttcgggcga
     2641 acggacggca gcaggaagac aagcggtttc gtattcctca caatgctgaa cacccattat
     2701 gttgtcttta gcacgcttga gtatacattt gactgaatgc gcacaggtcg aacataaacc
     2761 ccgtgtcttt ttggtcatat tgcccccctt atcttttgag ttattctcat gggcaaactg
     2821 cgtgccatat ggataaatgc taaagctaat tacaaggaag aaggaaagtg ttggaaatta
     2881 aaggctaaaa gaaacaagaa ttcgatacgg tgaagcaatt acagcgataa taggaacaaa
     2941 aaacagctag taaatgggga tattttcccc acctggggaa atattcctca atttcgttat
     3001 tggcttcttg ccgcggaaaa gactgtggtt tttttaaaca cttttgttgt ttttcttggt
     3061 tgctatgttg atttcactat cattcggctt gagcttgtct cgaagtgttt tgcggtcaat
     3121 acgtaagatt tctgctgcgc gtgatttatt gccgccgacg cttgccaaaa ctctacgtat
     3181 ataggcggct tcttcttcgg caagcggtcg aaaagggacc gaaccgggcg tgatggaaaa
     3241 acgcataacc gaaggcaggt cagtgatttc aattttttgt ccgtcggtca tgataatgag
     3301 tcgctggatt agattttcga gttctcgaat atttcccggc catgcatagc ccatgagggc
     3361 ttgcacaa
//
LOCUS       DAOSME010000151         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_30617_length_3368_cov_19.697555, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000151 DAOSME010000000
VERSION     DAOSME010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30617_length_3368_cov_19.697555"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="PLG98_11595"
     CDS             <1..2070
                     /locus_tag="PLG98_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HOE33952.1"
                     /translation="GRLSQIANINHQEGVDVLSITAEVDKGVFEVVPSAPLFNDIKGI
                     LELKRRQFSIKNIRAYFGKSPCTVEGNISDFARPEPNIYTAYMKVQPVRDDVVWLLGE
                     KTFNDMDFRGTSLLTLSGKGPDTGFRIRADWNLTDVAYAFPGVLEKPRSRKNQFTADL
                     VIDDQAVDFQSFKHVMPPVNVTGNMRLRYEGDSPASFSIQSGTFDIREAAPLLPLLQK
                     FDPTGAAQIAVTGQGNLNEPSSLQWKGNIALKNVSLDVSDKFSRMQGLTGSVSFKNRS
                     MQTSLFRTQIGRSKIQGNFWIEDFSDSRVFCRVNSEALKASDLGLKSAEGDVTLRDVK
                     GQLSFEDELIHVERLQFGAGTSTFILSGDITEFDLPKVTLELSSMYVHSEDLSRLMAL
                     SYPSKDQKAKSTMDLNAVFLLDGGTFGDAPFQKLYAVMKLKNDILDVDELEVGIFDGK
                     FKAKGRVVLPAAGPNRYDANITVDKISLDKLQQYLELGDRTVTGDLSLSANISMSGAN
                     AGELKQSAMGTVKVRAEKGVLKKFAVLSKIFSLLNIVQLAKLKLPDMATGGMPYSEIT
                     FHATFKGGVISSEDFFIDSNAMQISGTGSIDFVRKKLDFVAGVHPLQSLDLIASKIPI
                     AGWIITDERGKLITAHFKVDGTWDDPKVSTITAKSIGKGTLDIFRRIFQLPEKLVTDT
                     GEVILGH"
     gene            complement(2108..2995)
                     /locus_tag="PLG98_11600"
     CDS             complement(2108..2995)
                     /locus_tag="PLG98_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE33953.1"
                     /translation="MKLEKDLYAIDKTLLVQAGEPISRKTLQRVAALSGNITYVPIKD
                     TWLMKDLVETFKDERYTNILHPPDINRKIIRSIARIRMPEKILSELAYLKTRSPYTYQ
                     HIMVIAILAGKVSFDHSLKNKYMHDLAVLLSLFHDLGKSRIPLSILDKPTPITIKERR
                     ILTSHPLIGYVLLHYYFGKDHKKYSFSSFQHHERLDGSGYPLGIKTLNKYSQLIGVVD
                     ALDALISERPYRRKPFSLRAAIDLLLDEAERGRFRKSLVRTLIIYSRRENPGAKIVIA
                     QRGRDQAPLDNAYGKTARS"
BASE COUNT          794 a          749 c          962 g          863 t
ORIGIN      
        1 ggccggctga gtcagatcgc caacatcaat catcaggaag gcgtcgatgt gctttccatt
       61 accgccgagg tggataaagg tgtctttgag gtggtgcctt ccgcgccgtt gtttaatgac
      121 atcaaaggca ttctggagct caagagaagg cagttttcta tcaaaaacat ccgggcctat
      181 ttcggcaagt cgccctgcac ggtggaaggc aatatttccg atttcgcccg gcccgagccc
      241 aatatttata ccgcttacat gaaggttcag ccggttcgcg atgatgtggt ctggctgctg
      301 ggcgagaaaa ccttcaacga tatggatttc cgggggacct cgctgttgac gctttcgggc
      361 aaaggtccgg ataccggttt ccggatcagg gccgactgga atctgacgga tgttgcttac
      421 gcatttccgg gtgttctgga aaaaccgcgc tccagaaaaa atcagtttac ggccgatctg
      481 gtgatcgatg accaggcggt tgactttcaa tcgtttaagc atgtgatgcc gccggtgaat
      541 gttacgggga atatgaggct gcgctatgag ggggactcgc ctgcatcgtt ttccattcag
      601 tcggggacgt ttgatatccg tgaagccgca ccgctgctgc cgttattgca aaaattcgat
      661 ccgacgggcg cggcgcagat tgctgtgacc ggtcagggta atctcaacga gccgtcttcg
      721 ttgcagtgga aggggaacat tgctctgaaa aacgtttcgc tggatgtgtc cgacaagttc
      781 agccggatgc aggggctgac gggcagtgtg tctttcaaaa accggtcgat gcagacctcg
      841 ctgtttagaa cgcaaatcgg gcgcagtaaa attcagggca acttctggat tgaagatttc
      901 agcgattcca gagtgttctg ccgggtcaac agcgaggcgc tcaaagcgtc ggatctgggc
      961 ctgaaaagcg cagaaggcga tgtgacgctg cgtgatgtca agggacaatt atcctttgaa
     1021 gatgaactga ttcatgtgga gcgtctgcaa ttcggagcag ggacgtcaac ctttattctg
     1081 tcgggagata ttacggagtt cgatctgccg aaagtaacac tggaactgag ttccatgtat
     1141 gtccattccg aggatttgag ccgtctgatg gctttgagtt atccgtcgaa agatcaaaag
     1201 gcaaaatcaa ccatggacct gaatgccgtc ttcctgctgg atggcgggac cttcggcgat
     1261 gcgccgttcc agaaactgta cgcggtcatg aagctgaaga acgatattct ggatgttgat
     1321 gaactggaag tgggaatctt tgacgggaag ttcaaggcca agggcagggt ggttttgccg
     1381 gcggcaggcc cgaaccgtta tgacgcgaat atcacggtcg ataaaatatc gctggataaa
     1441 cttcaacagt atctggaatt gggcgatcgc acggtgaccg gagatttatc cctgtccgcc
     1501 aatatctcga tgagcggcgc caatgccggg gaactcaaac agtctgcgat gggaaccgtg
     1561 aaggtgcgtg ccgaaaaagg ggtgttgaaa aagtttgcgg ttctgtcaaa aatattttca
     1621 ttgctgaata ttgtgcaact ggccaagctg aagctgccgg atatggcaac cggcggcatg
     1681 ccctacagtg agatcacgtt tcatgcgacg ttcaagggcg gcgtgatatc ctcggaagac
     1741 ttttttattg acagcaacgc gatgcagatt tcggggacag gcagcattga ttttgtccgg
     1801 aagaagctcg attttgttgc cggtgttcat cctctgcaat cgcttgatct gatcgcttcg
     1861 aagattccca ttgccgggtg gattatcacc gatgagcggg gaaaattaat tacggcccat
     1921 tttaaagtgg atggaacctg ggacgatccg aaggtgtcca ccatcacggc gaaatccatc
     1981 ggcaaaggca cgctcgatat tttccgccga attttccagt tgccggaaaa gctcgtcacg
     2041 gatacgggcg aagtcattct ggggcattaa aaatgtttgc cgaaacgaaa catcggaaga
     2101 aaaattttca ggacctggct gtcttgccgt aggcgttatc cagtggcgcc tgatcgcgtc
     2161 ctctttgcgc aatgacaatt ttggcccccg ggttttcccg gcgggaataa ataatcagcg
     2221 tgcggaccag actttttctg aatctgcctc gttccgcttc gtcaagcagc aaatcaatgg
     2281 cggctctcag cgaaaagggt tttctcctgt agggcctttc cgagatgagc gcatccagcg
     2341 catccaccac cccgatcaac tgggaatatt tgttgagggt ttttatgccc agcggataac
     2401 cggacccgtc gagccgttca tgatgctgaa aagaagaaaa agaatatttc ttgtgatcct
     2461 tgccgaaata ataatgcagc agaacataac cgatcaacgg atgcgaggtc aggatccggc
     2521 gttccttgat ggtgatcggc gtcggtttgt ccaggatgct caacgggatg cgcgatttgc
     2581 ccaggtcgtg gaaaaggctc agcagtacgg ctagatcgtg catgtatttg tttttcagcg
     2641 aatgatcgaa ggataccttg ccggccagaa tggcaatgac catgatgtgc tggtatgtat
     2701 agggggagcg tgttttcaaa taagccagct cggataatat cttttccggc atccggattc
     2761 gggcgatcga gcggatgatc ttccggttga tgtccggagg atgaagaata ttggtgtaac
     2821 gttcgtcttt gaaggtctcg acgagatcct tcatcagcca ggtgtctttg atcgggacat
     2881 acgttatatt tcccgataat gccgccacac gctgcaaggt ttttctgctg atgggttcgc
     2941 cggcttgaac cagaagcgtt ttgtcgatgg catacaaatc tttttccagt ttcatatgtc
     3001 ttctccgatg acgataggca tagttgattt ggttttttct gtcaaatact ttgccggtga
     3061 tttttaaaaa ttctccggac ggtgcgtcaa aaaaaattga ttttaagagg caagatcagt
     3121 ataaggtttg tcggtctgat gttgaaatga actgtcggcg ttgtcgatga aagattaaac
     3181 gaggagcatg ccatgaacga ggtgaagaag gatcgtgaac attgggcgag tcgcgtcggt
     3241 tttattctgg cggcggcggg atccgctgtc gggctgggca acatctggcg gtttccctat
     3301 atgacggggg aaaacggcgg agcggcgttt attgtgattt atctgctggc cgttttttta
     3361 ctcggtta
//
LOCUS       DAOSME010000152         3316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_31295_length_3316_cov_22.173260, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000152 DAOSME010000000
VERSION     DAOSME010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31295_length_3316_cov_22.173260"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLG98_11605"
     CDS             complement(<1..895)
                     /locus_tag="PLG98_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOE33954.1"
                     /translation="MNIVISGSMAYDRIMDFPGYFSDHILPDKIHMLNVCFQVNGMKE
                     KFGGTAGNIAYALRMLGEHPIICATIGHDYQKYFKWLTKNGISTESIKVIDEEFTAGA
                     YITTDRSDNQITGFNPGAMKYQCKIDFDKFDSRETIVIVSPGNFNDMVEYPRACKAKG
                     IDYILDPGQSLPMWDKNDLIQAIEGCRILIVNDYELSLIMSKTGLNKNALLKMAGTVI
                     TTLGELGSQVSTLTSDIAIPPVKLKKVVDPTGAGDAYRGGLLSGLVQGKDIVTSAKMG
                     SVSASYAVETYGTQEYSYTSEE"
     gene            complement(977..2314)
                     /locus_tag="PLG98_11610"
     CDS             complement(977..2314)
                     /locus_tag="PLG98_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOE33955.1"
                     /translation="MPENNFDIIISGGTLLTMSAGMDIIEDCFVGIHDGIITDIRSLK
                     KTRSTSIRAHETINADGCIVMPGLVNTHTHLPMVCFRGLADDLPLMEWLTGHIWPAEQ
                     RFVSKKMVYDGATLAMAEMILSGTTTFCDGYFFEDSLAEAVHVAGMRAVVAQGFADFV
                     MPDSAAVKKMMAAANRFVKKWYPHAPLLTPAFFCHSPYTCSPATLSAVKQSAREHGIL
                     YLVHLLENKDEVHTIRQRYGRTPVQHLLNLGVLDEQTVAVHCNWLTSEDMKIFADLGV
                     KVSHNPESSMKLASGVAPVPDMLKEGISVGIGTDGCASNNDLDMFREMDTTAKIHKVI
                     SLDPTVMNAESVCRMATIGGAHVLGLDKRIGSIETGKQADIILVDMNQPHLTPVYHYY
                     SQLVYAARGADVKTSIVNGKVIMKDRRLLTIDVPTAMKNVRTVAGGIIRKDSH"
     gene            complement(2301..2693)
                     /locus_tag="PLG98_11615"
     CDS             complement(2301..2693)
                     /locus_tag="PLG98_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33956.1"
                     /translation="MKRKIIIAAFALSFLFLVSMICFSTAEVCYADAPKDVKIEYDSG
                     AQTLTVTITHKSAFPGFHHIKNVDVKKNAVSVSQNAYSSQPKEVPFSYVYKVAAAPGD
                     KLDVTVVCNMSGSKTATLTVPAAGNAGK"
     gene            2862..>3316
                     /locus_tag="PLG98_11620"
     CDS             2862..>3316
                     /locus_tag="PLG98_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOE33957.1"
                     /translation="MSAVILARLQFALTICFHFLFPAMTLGTTLIILISETLYLVKKD
                     ETYKKITDFLVRLLALIFVVGAATGIVMEFSFGTNWSGYSRAVGDIFGPILAAEGVLA
                     FFLESVFLGVLLFARNRVSPKVYWLSALLVFIGGHLSAFWIVVANSWMQT"
BASE COUNT          739 a          796 c          872 g          909 t
ORIGIN      
        1 attcttcgga cgtgtagctg tattcctgcg tgccgtaggt ttccacggcg taggacgccg
       61 aaacgctgcc catttttgcg ctcgtcacaa tgtccttccc ctgaaccaga ccgctcaaca
      121 gaccgccacg gtaggcgtct ccggcgcctg tcggatcgac cacttttttt aatttgaccg
      181 gagggatggc gatgtcgctg gtcagcgtgg agacttgcga acccagttct cccagcgtgg
      241 tgatgacggt tccggccatt ttcagcagcg cgtttttatt caggccggtt ttgctcatga
      301 tgaggctgag ttcgtaatcg ttgacaatca gaatccggca gccttctatg gcctgaatca
      361 gatcgttttt gtcccacatc ggcagagact gcccgggatc cagaatgtaa tcgatgcctt
      421 tggctttgca ggcacgcgga tactctacca tgtcgttgaa attgccgggc gagacgatga
      481 cgattgtttc ccgggagtcg aatttgtcaa aatcgatctt acattggtat ttcattgcgc
      541 cgggattgaa cccggtaatc tgattatcgg accggtcggt ggtaatgtag gcaccggcgg
      601 taaactcctc atcgatgact ttgatgcttt ccgtggagat gccgtttttt gtcagccact
      661 tgaagtattt ctggtaatcg tggccgatgg tggcgcagat gatgggatgt tcgccgagca
      721 ttctcagagc gtaagcaatg ttgccggccg tgccgccgaa tttttccttc atgccgttta
      781 cctgaaagca gacattgagc atgtggattt tatccggcag aatatggtcg gaaaaatatc
      841 cgggaaaatc cataattctg tcatacgcca ttgaaccgga aataacgata ttcataattt
      901 aaatcagtcc ttcagattat tttgaaaaca tataggaaat attgattttt gtgtcaatga
      961 cattgttgga caatattcag tgcgaatctt ttctgatgat gccgccggct accgtccgca
     1021 cattcttcat ggcggtcgga acatcgatcg tgagcaaacg ccggtctttc atgatcacct
     1081 tgccgttgac aatgctggtt ttgacatccg cgccgcgtgc ggcatagaca agttgagagt
     1141 aataatggta gacaggcgtc agatgcggct ggttcatatc cactaaaatg atgtccgctt
     1201 gctttcccgt ttcgatggaa ccgattcgtt tgtccagtcc cagaacgtgt gcccccccaa
     1261 tggtggccat ccggcagacg gattcagcat tcatgaccgt cgggtccaga gaaattactt
     1321 tatgaatttt tgccgttgtg tccatttccc ggaacatatc caggtcattg ttgctggcgc
     1381 agccgtccgt tccaatgccg acggatattc cttccttcaa catgtcggga accggcgcaa
     1441 cgccggaggc gagtttcatg gaactttccg gattgtgtga aacctttact cccagatcgg
     1501 cgaaaatttt catatcttct gatgttagcc agttgcagtg aacggctacg gtttgttcgt
     1561 ccagcacgcc caggttcagc agatgctgga ccggtgtccg gccgtagcgc tgccggatgg
     1621 tgtggacttc gtccttgttt tccagtagat gaaccaaata taaaataccg tgttcgcggg
     1681 cggattgctt gactgcggag agtgttgcgg gagaacaggt atagggcgaa tgacagaaga
     1741 aagctggagt caagagcggc gcatggggat accatttctt tacaaaacgg ttggctgccg
     1801 ccatcatctt ttttacggcc gcgctatccg gcatgacaaa atcggcaaag ccctgggcga
     1861 cgacagcgcg catcccggcg acatggacgg cctcggcgag gctgtcttca aagaaatatc
     1921 cgtcgcagaa ggttgtcgtt cccgacagaa tcatttcggc catggccagc gttgcgccgt
     1981 catacaccat ttttttgctg acaaaacgct gttccgccgg ccagatatgc ccggtcagcc
     2041 attccatgag aggcagatcg tcggccagtc cccggaaaca gaccattggg agatgtgtgt
     2101 gcgtattcac caggccgggc atcacgatac agccgtccgc gttgatggtt tcgtgcgccc
     2161 ggatggaggt cgaccgggtt tttttcaggg aacggatatc ggtgatgatg ccgtcgtgaa
     2221 tgccgacaaa acaatcctcg atgatgtcca tgccggcgga catggttaat agtgtgccgc
     2281 cggaaatgat gatgtcgaaa ttattttccg gcatttcctg ctgcgggcac cgtcaatgtt
     2341 gccgtcttgc ttccggacat gttgcaaaca accgttacat ccagcttatc gccgggagcg
     2401 gcagccacct tatatacgta gctgaacggc acctctttgg gttgcgatga gtaggcgttt
     2461 tgactgacgg aaaccgcatt ctttttcacg tcgacatttt taatgtgatg aaatccgggg
     2521 aacgcggatt tatgcgtgat cgtgaccgtc agggtttgcg ctccggaatc gtattcgatt
     2581 tttacatcct tgggcgcgtc ggcgtaacag acttcagcgg ttgaaaagca aatcattgat
     2641 acaagaaata aaaaactcaa agcaaaagca gcgatgataa tttttctttt catggcagac
     2701 ctcctgatct ttaatgttct gtgctgttca cgttaaatga taatatattc gttgcgcaaa
     2761 taacagcgaa aaattatgag ttgaaaagtt gcggataatt ggctagtgtt taaaagaatt
     2821 gaacagtaca agccgacttc aactgccgaa atgaggtgct tatgagcgca gtcatccttg
     2881 cccggttaca gtttgccctg acgatatgtt ttcattttct tttccccgcc atgacgctgg
     2941 gcacaactct gattatcctg atatccgaaa cgctttatct ggtgaagaag gacgagacgt
     3001 ataaaaaaat aacggatttt ctggtcaggc ttctggcgtt gatttttgtt gtcggggctg
     3061 caacaggtat tgtcatggaa ttttcattcg gcaccaactg gtcgggatat tcccgcgcgg
     3121 tcggagacat tttcgggccg atcctggctg cagagggtgt attggcgttt tttctggaat
     3181 cggttttcct tggcgtactg ctttttgccc gcaacagagt gtccccgaaa gtatactggt
     3241 tatccgctct gctggttttt atcggcggcc atctttccgc cttctggatc gtggtggcca
     3301 attcctggat gcagac
//
LOCUS       DAOSME010000153         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_31578_length_3292_cov_19.108125, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000153 DAOSME010000000
VERSION     DAOSME010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31578_length_3292_cov_19.108125"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1204)
                     /locus_tag="PLG98_11625"
     CDS             complement(290..1204)
                     /locus_tag="PLG98_11625"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE33958.1"
                     /translation="MKKNIANVQASVRRRLQNVAKETHRPFAEVLQYYGMERFLFRLC
                     KSAHARQFILKGALMFTAWHLPQRRTTRDIDLLARCDNRTDNIEKIIKDICKTSVPSD
                     GLVFDHQTIHSQRIKEDADYEGVRVRFTGLLERARIPMQIDIAFGDTVYPVPGIVDFP
                     VILDFHKPRLKGYPPESVISEKFEAMVKLGLLNSRMKDFYDIWLMTRQFDFEGMNITA
                     ALSKTFEHRKTPLPVKRPLFAEEIYDEKSDRQTLWTAFLRKNDIKHAPKKLSAAAKVI
                     EKFLLKPLEAIHKNLEFKQEWNAAGKWQ"
     gene            complement(1201..1803)
                     /locus_tag="PLG98_11630"
     CDS             complement(1201..1803)
                     /locus_tag="PLG98_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33959.1"
                     /translation="MRAKKQNLIDYFHKKGGIVRFSAMRKAGFHPDTIAALEKNGQIS
                     KISRGLYQLAGYEPAAHPDLVMVSLLAPRVVICLISALAFHEITSEIPKYVEIAIPKQ
                     SHANIITYPPVRVYRFDHQAWREGVEEHRIDGHTVRVYSPAKTIADCFKFRNKIGVNV
                     ARDAIKTAIAEKRVRPQEIMKYAKICRVSNIIKPILETLI"
     gene            complement(2129..3100)
                     /locus_tag="PLG98_11635"
     CDS             complement(2129..3100)
                     /locus_tag="PLG98_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOE33960.1"
                     /translation="MASKIILERFLWFQNEIKQGRYPNTKTLAEKFEITRKTAQRDIE
                     FMRDRLNAPLMYIPGHRGFTYEDHTYDLPGFWLGEEALISLLLAYRLVSAVPDKSLKT
                     SLRSFLEQLLTKISHSQTMTVKDLNSKISVRNIAYSKTSEKIYHAMLEALLNAGPVKI
                     EYHSPHNSQITTRDILPLHLLHYMGTWHIIAHCALKNELRDFVLSRIQTIAPCAHKIT
                     ARFSSGHVRDYIRKTFGIFHGKQTKSVCLCFSKDVSPWISEQVWHPAQKTFIKKDGRL
                     CLTIPVADFREIKREILKYGSQVEVISPKALRNEIRNEIGKMKKIYR"
BASE COUNT          771 a          717 c          800 g         1004 t
ORIGIN      
        1 accaccccca atttatgtcc catcaaaacg ttgctcaaaa gatattttat ggatttaaaa
       61 tgataaaacg ttccggaaat ttccgagaca gcgtttattc atttcaaaac atcgggggtt
      121 tgatggtttg ttattatact tctttaaaat attttcaatc cctcgaaaga gggatattga
      181 taattatcac cgtatccggt gaaacgattc tggattcccg ccgaaagcat gcgggaatga
      241 cttcattttt tttcagactg atcggggaac aatccaaaca taatttccct tattgccatt
      301 tgccggcggc attccattct tgcttgaatt ccagattctt gtgaatcgcc tccagtggct
      361 ttagcaggaa tttttcaatt accttggccg ctgccgatag ttttttcggc gcgtgtttta
      421 tatcgttctt ccttaaaaat gccgtccaca atgtctggcg atcggatttt tcatcataaa
      481 tttcctctgc aaacagtgga cgtttcacag gcagtggcgt tttacgatgt tcaaaggttt
      541 tgcttaaagc cgcggtaata ttcatgcctt caaaatcaaa ttgccgtgtc atcagccaga
      601 tatcatagaa atctttcata cggctgttta acaagcccaa tttcaccatg gcctcgaatt
      661 tttcactgat gacactttcc ggcggatatc ctttgagacg cggtttgtga aaatcgagga
      721 tgaccgggaa gtcaacgatt ccagggactg gataaaccgt atcgccaaag gcgatgtcaa
      781 tctgcatggg tatgcgcgca cgttccaata atcctgtaaa ccggacccgc acaccttcgt
      841 aatcggcatc ttctttaatc cgttgactgt gtatcgtttg atgatcgaaa acaaggccgt
      901 cggaaggcac gctcgttttg caaatatctt ttatgatttt ctcaatgttg tctgtccggt
      961 tatcgcatcg cgccagtaaa tcaatgtcgc gggtcgtgcg ccgctgaggc aggtgccagg
     1021 cggtaaacat tagcgctcct ttaagaataa actgacgcgc atgggcggat ttgcacagcc
     1081 ggaaaagaaa tctttccatg ccataatact gcaagacttc cgcgaagggc cggtgcgttt
     1141 ctttggcaac gttttgaagc cttctccgga cggatgcctg cacattggcg atattctttt
     1201 tcatatcaat gtttccagaa tcggcttgat gatgttgctt acgcgacata ttttcgcgta
     1261 tttcatgatt tcctgcggcc tgacacgttt ttctgcaatg gcggttttga tggcgtccct
     1321 ggctacattg acgccgatct tgtttctgaa tttgaagcaa tccgctattg tttttgccgg
     1381 tgagtaaact ctgaccgtat ggccgtctat acgatgttct tcaacgcctt ctcgccatgc
     1441 ctgatgatca aaccggtaaa ctcgcaccgg cggatacgtg atgatattcg cgtgggattg
     1501 tttgggtatg gcaatctcca cgtatttggg gatttcgctg gtgatttcat gaaaggccag
     1561 cgcggaaatc aggcatatca cgacgcgcgg cgccagaaga gagaccatga ccagatcggg
     1621 atgcgcggct ggttcgtaac cggcaagttg atacaggccc cggcttattt tggagatttg
     1681 cccgtttttc tccagcgcgg caatggtatc cgggtgaaac ccggcttttc gcatggccga
     1741 gaaacgaaca atgccgcctt ttttatggaa ataatctata agattttgtt tttttgcgcg
     1801 catttccttt ccgtatataa tgtcaattgt tttaaatatg tgtatatgat ttatacggta
     1861 ttgtcaatac ggatgttcta atgtcctgcc tgaccgttca tcaatatttg tttgtatagg
     1921 atccggtaaa agcctcgtac accataaaat cccccctacg cggtgctccg gggatagttc
     1981 ggcttacgct tcatactttg ttgcattcgt cttcagcttg cctcacgacc ccttcatccc
     2041 cctttacaaa agggggagat taaggcctgg atttacccta acgggcacca tggcccgaca
     2101 agaccattcg ggaatgacag gcaagatgtt atctgtatat ttttttcatt tttccaatct
     2161 cgtttcttat ttcgttgcgc agggctttgg gggatatcac ttccacctgc gagccgtatt
     2221 tgagaatttc tcttttgatc tcccggaagt cggcaacggg aatggtcagg cagagcctgc
     2281 cgtctttttt gatgaatgtt ttttgcgccg gatgccaaac ctgttcggaa atccaggggg
     2341 aaacatcttt ggaaaaacac aggcagacgg attttgtctg ttttccatga aagatgccga
     2401 aggttttccg gatatagtcg cgcacgtgtc cggaagagaa tcgggcggta attttatggg
     2461 cgcagggggc gatggtttgt atgcgcgaca aaacaaaatc ccgcagttca ttttttaacg
     2521 cgcaatgggc gataatatgc caggtgccca tgtagtgcag cagatgcagc ggcaaaatgt
     2581 cgcgggtggt gatttgggaa ttgtgcggcg aatgatattc tattttcacg ggcccggcat
     2641 ttaaaagagc ttccagcatg gcatgatata ttttttccga cgttttggaa tatgcgatgt
     2701 tgcggacgga tattttgctg ttcaggtctt tgacggtcat ggtttgggaa tgcgatattt
     2761 ttgtcagcag ttgttcaaga aacgagcgca gggatgtctt gaggcttttg tccggcacgg
     2821 cggaaaccag acggtatgcc agaagaagtg aaattaatgc ttcttcaccg agccagaaac
     2881 ccggcaggtc ataggtatgg tcttcgtagg taaatccgcg atggccgggg atatacatca
     2941 gcggcgcatt cagcctgtcg cgcatgaatt caatatcgcg ctgggcggtt ttgcgggtga
     3001 tttcaaattt ttccgcaagg gttttggtgt tgggatacct tccctgtttt atttcgtttt
     3061 gaaaccagag aaatcgttcc agaatgattt ttgaagccat gtcccctcct ttgccggatg
     3121 aatttttaca gactgattga attgtttcgg gtgcaggcgg ctttcctctc tgtttggttt
     3181 atgtttattt ttttaggtga gagaggattc cggttatcaa tagtgaatta tgccggtaag
     3241 cgcaagaatt ttttgaattg gcttttttga ggacgaaaaa aaaccgggga tg
//
LOCUS       DAOSME010000154         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_32951_length_3189_cov_14.611040, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000154 DAOSME010000000
VERSION     DAOSME010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32951_length_3189_cov_14.611040"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="PLG98_11640"
     CDS             <1..1664
                     /locus_tag="PLG98_11640"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOE33961.1"
                     /translation="NPGVIADCTGDNAGDSVGPTADGFETYGVTGVALITFLALALAK
                     NPEMGGKLIIWIFAMRILMILTALLSYFVNNGISKAAFAGKKEFNFEHPLTNLVWITS
                     ILCIVVTFVASYYLLGDLNAPYTSLWWALSIIISCGNLAGALIPEFTKIFTSTSSRHC
                     EEVVNASKQGGPSLNILSGLVAGNFSGFWLGLVVAFLMYVAYMVSGVESVRALMPPAF
                     TFAAPVFAFGLVAFGFMGMGPVTIAVDSFGPVSDNAQSIYELSMIESRPDAKAVVKKE
                     YGKEPDFELAKHFLESADGAGNTFKATAKPVLIGTAVVGATTMVFGIIIMLEGIFGNV
                     VQQLSLVQPDIIVGLILGGCVIYWFTGASMQAVTTGSYQAVVYIKKNINLDKKEASIE
                     DSKKVVQICTQYAQKGMINIFIVIFFMCLALAFFNPYFFIGYLFGIAFFGLFQAVFMA
                     NAGGCWDNGKKIVEVDLKMKNTPLHEASVVGDTVGDPFKDTSSVSLNPVIKFTTLFGL
                     LATEIAVTMTNVNLKYALAAIFFFIALVFVYRSFYSMRISAEKLG"
     gene            complement(1727..2812)
                     /locus_tag="PLG98_11645"
     CDS             complement(1727..2812)
                     /locus_tag="PLG98_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33962.1"
                     /translation="MCQRTKRKCSVVTVCFLLLISLFLVSTLSYAAVKVTPPGMINSV
                     LGPISPEKLGTTLVHEHFVFAYPGWLADDTIAPVDPKAIFKTDLAVIKAAQKYGIKTI
                     IDATTNDVGGRDPALYKKLALKTRINIICSTGLYTEHEGSPAYWTTKAIWGMDISKMM
                     SELFIKEITQGIGKTGVKAGVIKIGSGPKMSAYETAVHKAAVVAQKATGVPIITHTEG
                     PTGGVEQADLLLAEGANPKKVMIGHVSNSKDIEYHKAILAKGVYIAFDRIGLDIITPT
                     DVNVKNIAELCKQGYTKQIMLSHDTVNVWLGRQTVLPEKFLPAFANWRIDHISQNVLP
                     ALKAQGVTDEQIKTMMVENPKNLFLGK"
     gene            complement(2906..>3189)
                     /locus_tag="PLG98_11650"
     CDS             complement(2906..>3189)
                     /locus_tag="PLG98_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33963.1"
                     /translation="PKLNCFGVNASKPLSYWAAKGWIHPDDPRGWFQWYCRYYLGRRC
                     PDDERQIKRWRAMQRHISQLTKHCPAGDLSCRRKQRQALLHWAYDSRKM"
BASE COUNT          686 a          798 c          839 g          866 t
ORIGIN      
        1 aaaacccggg cgttatcgcc gattgtacgg gtgacaacgc gggtgacagc gtcggcccca
       61 cggcggacgg ttttgaaacc tacggtgtta ccggtgtcgc cctaatcact ttcctggcac
      121 tggctttggc caaaaacccg gaaatgggcg gcaaactcat tatctggatt ttcgccatga
      181 gaatccttat gatcctgacg gctcttcttt cttatttcgt caataacggc atcagcaaag
      241 ctgccttcgc cgggaaaaaa gagtttaact ttgaacatcc gttaaccaat ctggtatgga
      301 ttacttccat cctgtgcatc gtcgtaacat ttgttgccag ttattacctc ctgggcgacc
      361 tgaatgcccc ttacacaagt ctgtggtggg cgttgtccat catcatcagc tgcggaaacc
      421 tcgccggtgc attgattccg gaattcacca agatattcac cagtacctct tcccgtcact
      481 gcgaagaagt cgttaatgcc tccaaacagg gcggcccttc gctgaatatt ctttccggtc
      541 tggttgcggg taatttctcc ggtttttggc tgggtctggt tgttgcgttc ctgatgtacg
      601 tcgcttatat ggtttcggga gttgaatctg tcagagctct gatgcctccg gcctttacct
      661 ttgcggcgcc ggtgtttgct tttggtctgg tggctttcgg tttcatgggc atgggccctg
      721 tgaccatcgc cgtggacagt tttggtcctg tttcggacaa tgctcagtcg atttatgaat
      781 tgtcgatgat tgaatcgcgt cccgatgcaa aagccgttgt gaaaaaagaa tacgggaaag
      841 aacctgattt tgaactggcc aagcacttcc tggaatctgc agacggtgcg ggcaatacct
      901 ttaaagcaac agccaagcct gtgttgatcg gcaccgccgt tgtcggcgcc accaccatgg
      961 tgttcggcat catcattatg ctggaaggca tcttcggcaa tgttgttcaa cagctttctc
     1021 tggtccaacc ggatattatt gtaggcttga ttctgggcgg ttgcgtcatt tactggttta
     1081 ccggcgcgtc catgcaggcc gtaacgacag gctcctatca ggctgttgtg tacatcaaaa
     1141 agaacattaa tctggacaag aaggaagctt cgattgaaga ctccaagaag gtcgttcaga
     1201 tttgtacgca gtatgcgcag aagggcatga tcaacatctt catcgtgatc ttctttatgt
     1261 gtctggcgct ggccttcttt aacccctact tcttcatcgg ttatctgttc ggtatcgcct
     1321 tcttcggcct gttccaggcg gtctttatgg ccaatgccgg cggttgctgg gataacggca
     1381 agaagattgt tgaagttgac ctcaaaatga agaacacgcc gctgcatgaa gccagcgtag
     1441 tcggcgacac cgtcggcgac cccttcaaag atacgtcttc cgtttctttg aacccggtta
     1501 tcaaattcac gaccctgttt ggtcttctgg caacggaaat tgccgtgacc atgacgaacg
     1561 tcaatttgaa atatgctctg gcggctatct tctttttcat cgccctggtt ttcgtgtatc
     1621 gttcgttcta cagcatgaga atctccgctg agaaactggg ctaataattt tacatcacct
     1681 ttaaacaaaa gggcgctccc tcaaaaggag cgcccttttt tataccttat tttcccagaa
     1741 ataaattttt tggattttcc accatcatgg ttttgatctg ttcatcggtg acgccctggg
     1801 ctttcagcgc gggcagaaca ttctgcgaga tatgatcgat ccgccagttg gcaaacgcgg
     1861 gcaggaattt ttcaggcaat accgtttggc ggcccagcca gacattcacg gtgtcatgcg
     1921 aaagcataat ttgctttgtg tagccctgtt tgcaaagttc cgcgatgttc ttcacgttga
     1981 catccgtggg cgtgataatg tccagtccga tgcggtcgaa cgctatataa acgcctttgg
     2041 ccagaatggc cttgtgatac tctatatctt tggaattgga gacatggccg atcatgactt
     2101 ttttgggatt cgctccctcg gccagcagca gatccgcctg ttcgacgccg cctgtcgggc
     2161 cctcggtgtg tgtgatgatc ggaacgccgg tcgccttctg ggcgacaacc gccgccttat
     2221 gaacggcggt ttcgtaggcg gacatcttcg gaccggagcc gatcttgatc acgcccgcct
     2281 tgacgccggt tttgccgatg ccctgcgtaa tctctttgat gaaaagttcc gacatcatct
     2341 ttgaaatatc cataccccag atggcttttg ttgtccaata ggccggagag ccttcatgct
     2401 ccgtgtagag gcctgtggaa cagatgatgt tgatcctggt tttcagagcc agttttttgt
     2461 acagtgcggg gtctcgtcct ccgacatcat tggtagtcgc gtcaatgatg gtcttgatgc
     2521 cgtatttctg cgccgccttg atgacggcca gatcggtttt gaagatcgct ttaggatcaa
     2581 cgggtgcgat cgtatcgtcg gcaagccagc cgggataggc aaacacaaaa tgctcatgca
     2641 ccagagttgt tcccagtttt tcgggagaaa tgggtcctag cacggagttg atcatgcccg
     2701 ggggcgtcac cttgacggct gcataagata gtgtggaaac caggaaaagg gaaatcaaca
     2761 aaagaaaaca tacggtaacg acggaacact tacgcttggt tcgttgacac atgatgaatc
     2821 ctccttttca aatgttctaa ccggccgcaa gcggcaagtc gaaaaaacga aaagattgga
     2881 aacaacaata ccagccggaa tccagtcaca ttttccgact gtcgtatgcc cagtggagca
     2941 gggcctggcg ttgttttcgc ctgcaggaga ggtcgcccgc ggggcagtgt ttggtgagtt
     3001 gggagatgtg ccgctgcatg gctctccagc gtttgatttg tctttcgtcg tcggggcagc
     3061 ggcggcccag gtaatagcgg cagtaccact gaaaccaccc ccggggatcg tcgggatgaa
     3121 tccagccttt ggccgcccag taggagaggg gtttggaggc gttgacgccg aagcagttga
     3181 gcttcggat
//
LOCUS       DAOSME010000155         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_33247_length_3168_cov_40.628333, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000155 DAOSME010000000
VERSION     DAOSME010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33247_length_3168_cov_40.628333"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            797..1585
                     /locus_tag="PLG98_11655"
     CDS             797..1585
                     /locus_tag="PLG98_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33964.1"
                     /translation="MKNFLKKISKLPESSFFGISIVCCFVILTFHVCSSMGAYRAYYM
                     QPVASMFFLIPAIQISIRNNKRFYINALCIFLIVSNLLIQNIFSYSQPIKTSFTEAFI
                     SLPLRGLFYEKEYVLAQNEFVQEASRHIQPGSKIFMPTQEYGLLLALNARPQYVSYCI
                     PANYLDKLRKGDRPEWILLHPHPWWEAVIRQENNIRFLPEKDLIFAPISSLRYIKDHL
                     QPNSVIYAYIDIVYFLRKIVFPKEYELVIKHNHYHLYRYKGQAK"
     gene            1612..2019
                     /locus_tag="PLG98_11660"
     CDS             1612..2019
                     /locus_tag="PLG98_11660"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOE33965.1"
                     /translation="MKWPKEFIRYAVVGAAANIFGFLLYALFTELGVSPVLTISIFYP
                     VHIGIAFYLNKRWSFNFKGRLSASAVRYLAAYAGCYGLNVAMLKFFNGYLGYSHLIVQ
                     AIAIVVIAVLLFLAQKMWVFRAQGGSFPFERSL"
     gene            2106..2897
                     /locus_tag="PLG98_11665"
     CDS             2106..2897
                     /locus_tag="PLG98_11665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE33966.1"
                     /translation="MPIFASVLANSSSGFKASYFAPLAAREASHFWFRARNALIVWAL
                     AKYGANVTSFMEIGCGTGFVLQGIARRFPAFRLVGSEIFAEGLTFAAERIPGGEFLQM
                     DAREIPFLEEFDAIGAFDVIEHIEEDETVLKQIQCALKPGGLLLLTVPQHRWLWSAVD
                     DHAMHVRRYEARELHAKVVRAGFRVVRSTSFIVSLLAPMFAYRLMQRGEGNTFNPHEG
                     LKMNPILNKIFEQCLYLERVLIQGGISFPIGGSRLVVAIKKATTD"
     gene            2916..>3168
                     /locus_tag="PLG98_11670"
     CDS             2916..>3168
                     /locus_tag="PLG98_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE33967.1"
                     /translation="MDSIPFNKPFIAGKELFYIAQAVIENRHTAGDGPFTKKCQQWLE
                     ERLGCKKALLTHSCTAALEMAAMLAGIQPGDEVIMPSFTF"
BASE COUNT          774 a          668 c          690 g         1036 t
ORIGIN      
        1 atattataca ttgtctcgtc tccttgttat aatttacctt tatcttttta tttatttggt
       61 tagtcgagaa gcagacacgg tggctcccta tttttttttg ggggcgttaa attcaattct
      121 tatattctgt ttacctcaat tagtcattta ttgtgccatc ccgttgattt atttttcgat
      181 agaatctttt gttttcaaat cgcatgaatt ccgttggaag gaatttctct ttattatagg
      241 cggcttttcg ttttctctat tgctgatgct cgcagtattt aacatgtttg gatatttcga
      301 atatcctgaa tcattttttt cctttcatgc taatgcaatg tcttttttcg atcttttgta
      361 tagcaattat catgatcttt ttcagttgca ttggttcgac atcgtctgga tcatattgat
      421 tgttttatta tttatggatg agaaaaggtt taaatttaaa agactcctgt tctgtctgac
      481 actttgcttt ccgatatgct atcttatctt gaagatttcg tccattggtg aaaaagatgt
      541 tttcaatatc tggcacatgc agttcttttt atacagcgat atgaatcgaa cgattatatt
      601 gtttccttgt ttccttgcca ttttcctcat aatttatcac ttctatccgt tgtgtaaaaa
      661 tatctttctt aagagaaaca ttatttttgg gaattcaaaa gtcagaatgt ctttcacgtt
      721 taagatgtca cctaaacatg gacatggtga attatctcaa gatcttacga ccgactcgca
      781 aattcggtgt catccgttga aaaatttttt aaaaaaaatc tcaaagcttc ctgaatcaag
      841 tttttttgga attagcattg tttgttgttt tgttatattg acatttcatg tctgttcctc
      901 catgggagct tatagagcat attacatgca gccggtagcg tcgatgtttt ttttgattcc
      961 tgcaattcag atctcaatcc gtaataataa acgcttctat atcaacgcct tgtgcatttt
     1021 tctcatagtg tcaaatctct tgatccagaa tattttctct tattcccagc cgattaagac
     1081 ttcatttaca gaagctttca tttccctccc gttgcgcgga ttgttttatg aaaaagaata
     1141 tgtactcgca cagaatgaat ttgttcaaga agcatcccga catatccaac ctggaagcaa
     1201 gattttcatg ccgactcagg aatatggttt gcttcttgct ctaaatgcac gacctcaata
     1261 tgtttcatat tgtattccag cgaactattt ggataaattg cgaaaaggag atcgaccgga
     1321 atggattctt ttgcatcccc atccctggtg ggaagcagtt atccggcaag aaaataatat
     1381 tcgatttctt ccggaaaaag acttaatatt tgcaccgatt tccagcctgc gttacataaa
     1441 agatcatttg caaccaaata gcgtcattta tgcatatatt gacatagttt atttcttgcg
     1501 aaaaatagta tttccaaaag aatatgaatt ggtcataaaa cataatcatt atcatcttta
     1561 tcgatacaaa ggacaagcaa agtaaaagaa aataaggatt gggactgaat tttgaagtgg
     1621 cctaaagagt ttattcgtta tgccgttgtc ggtgccgcgg cgaatatctt cggattttta
     1681 ctttatgccc tgtttacgga actcggtgtt agtccggtgc tgaccatcag catcttctat
     1741 cccgtccaca tcgggatcgc tttctatctc aacaaaaggt ggtcgttcaa ttttaaagga
     1801 cgcctttcgg cgtcggctgt ccgatatctg gcagcctatg ccggatgcta tggtttgaat
     1861 gtagccatgc tgaaattctt taacggttat ctgggctatt ctcatctgat cgtccaggcg
     1921 attgccattg tggtcattgc cgtgctgctc tttttggcgc agaaaatgtg ggttttcaga
     1981 gcgcagggcg gatcatttcc ctttgagcgg tctttatgaa acggtgtctt cattgcagtc
     2041 ggtcctttga aagccaagac tggctttgtc catattgttc ctggcagccg gacaagagcg
     2101 gttccgtgcc gatattcgct tcggtactgg ccaacagcag cagcggcttc aaggcctctt
     2161 actttgcgcc gctggccgcc cgggaagcga gccatttctg gtttcgagca cgtaatgcat
     2221 tgatcgtctg ggctctcgca aaatacggtg caaacgtcac atcgtttatg gaaatcggct
     2281 gtggcaccgg atttgtgctg cagggcattg cccgccgttt tccggctttt cgtctggtcg
     2341 gcagtgagat ttttgcagaa ggcctcacct ttgcggcgga aaggattccc ggaggggaat
     2401 ttctgcagat ggatgcgaga gaaatccctt tcttggaaga atttgacgct atcggcgctt
     2461 ttgatgttat tgaacatatc gaagaagacg aaaccgtgct gaaacagatc caatgtgcgc
     2521 tgaagcctgg cggcctgctt ttgctcaccg tgccgcagca tcgttggctt tggagtgcgg
     2581 tggacgatca tgccatgcat gtccggcgtt acgaggctcg tgaactgcat gccaaagttg
     2641 tgcgcgccgg ttttcgcgtt gtgcggagca cctctttcat tgtaagctta ttagccccca
     2701 tgtttgccta tcggctgatg cagcggggag agggaaacac gttcaacccc catgaaggat
     2761 tgaagatgaa tcccatcctg aacaaaatct ttgaacaatg tctttatctg gagcgggtgc
     2821 tgattcaagg ggggatctca ttcccgatcg gcggttcccg gctggtggtg gcaatcaaaa
     2881 aagcaacgac agactgatga tttgagaggt aacccatgga ttcaattccc ttcaacaaac
     2941 cattcattgc cggcaaagag cttttttaca ttgcccaggc ggttattgaa aatcgccata
     3001 ctgcgggaga cggccctttc acgaagaaat gtcagcaatg gctggaggag agacttggct
     3061 gcaagaaggc gcttctcacc cattcctgca cggctgccct tgaaatggcc gctatgctgg
     3121 cggggattca accaggagac gaagtgatca tgccttcctt tacctttg
//
LOCUS       DAOSME010000156         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_33546_length_3146_cov_15.552896, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000156 DAOSME010000000
VERSION     DAOSME010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33546_length_3146_cov_15.552896"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLG98_11675"
     CDS             complement(<1..1004)
                     /locus_tag="PLG98_11675"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE33968.1"
                     /translation="MKKRIIFVVFIIFFLIVGALVYFGQKSNREKELVYSGTIDAIQA
                     NLSFQVAGRVSQVFVQEGQSVSQNQMIAELDRSEFESRFEQARANLERAQKTRRQLET
                     VLELSRKTLPAEVARAKAGVQSAKDSLKDAEKNYRRFEELFGKNVVSEKERDALRLQY
                     DVAQSRLAESESVLKLAQGNLTRIDAVKQDIDVAAAQINAAAASLNQAEIQLNYTQLK
                     SPANGVVTSRNIEPGETVTPGREVITVSDLSRVDLKIFVDETEIGKVKPGQKVDVQVD
                     TFPGKTYTGYVSFISPEGEFTPKVIQTTKERVKLVYLVKVSIGNPHLELKAGMPADAW
                     L"
     gene            complement(1032..1613)
                     /locus_tag="PLG98_11680"
     CDS             complement(1032..1613)
                     /locus_tag="PLG98_11680"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE33969.1"
                     /translation="MAAARTVFAQKGYNGARIDEIAARANVNKATIYYQIGDKDTLYT
                     GALHQVIGHVARDIADAVSRKVHPEEKMKAYITFIAGAVEANPDLPPMMMREIASGGA
                     TLPRLVIEDIASVMSILAGILEAGKEQGIFIETSPFLIHMMIMGTILFFKKGSPIKDK
                     QSWLPEALLAYDQKISANLGEEVASLVLKAIKQ"
     gene            complement(1861..2805)
                     /locus_tag="PLG98_11685"
     CDS             complement(1861..2805)
                     /locus_tag="PLG98_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE33970.1"
                     /translation="MSKTLGKIFTILAVVILAGVFNANAAEEARDGRFIAYDDGTVWD
                     SQSNLLWASHDNGSNINWSDAEEYCKTYDGSGYKGWRLPTKDELLTLYDRSIFGNNGY
                     RLTKLITLSGSYPWTSDVQGSEALVVGFDKDRQMWFPESVNHKSRNRVLPVRFGKEGK
                     MTVAEPKQEEVPPLPPPPPPETIAPPPPPPPVQEKVSISLNIEFDTAKSFIKKKYHNN
                     IKQVADFMQTYPDTNVVIEGHTDNVDRFNNPENNIKLSQARADSVRKYLIDKFGIDES
                     RITAVGYGPSRPIADNNTPEGRMKNRRVEAVIEKMVTK"
BASE COUNT          694 a          787 c          748 g          917 t
ORIGIN      
        1 agccaggcat ctgccggcat gccggctttg agttcaagat ggggattgcc gatggatacc
       61 ttaaccagat aaacgagttt taccctttcc ttggtcgtct gaataacttt cggggtaaat
      121 tccccttccg gagaaataaa cgacacatag cccgtgtagg ttttccccgg gaaggtgtca
      181 acctgaacat cgactttctg ccccggcttg accttgccga tttccgtttc atcaacgaag
      241 attttcaaat caacgcggga cagatcggaa acggtaatga cttccctgcc gggcgttacc
      301 gtttcccccg gctcgatatt gcggctggtg acgacgccgt ttgccggcga tttcaattgc
      361 gtgtagttca actggatttc cgcctgattt aaggaagccg cagccgcgtt gatctgtgcg
      421 gccgccacgt cgatatcctg tttgaccgca tcgattctgg tcagattccc ctgcgcaagc
      481 ttcagaacgg attcgctttc agccaggcgt gattgcgcaa cgtcatattg cagtctcagg
      541 gcgtcgcgtt ctttttccga aaccacgttc ttgccaaaca gctcttcaaa gcgcctgtaa
      601 tttttttccg catccttcaa ggaatctttt gcgctctgga cgccggcttt agcccgggca
      661 acttccgcgg ggagcgtttt ccggctgagt tccagcaccg tctccagttg tcttctggtt
      721 ttttgcgcgc gttccagatt cgcccgggcc tgctcgaagc gggattcgaa ttccgaacgg
      781 tccagttcgg cgatcatctg attttgcgag accgactggc cttcctgaac gaaaacctgc
      841 gaaacgcggc cggctacctg aaacgacaga ttggcctgga ttgcgtcgat cgtgccggaa
      901 tatacgagct ctttctcccg gttacttttc tggccgaaat aaacgagcgc tccgacaatc
      961 agaaagaaaa tgataaagac aacaaaaatg attctttttt tcaatgccga cctccgtttt
     1021 gattgagatg gttattgttt tatcgctttg agcactaaag acgccacttc ctcccccagg
     1081 ttcgcgctta ttttttgatc atacgccaga agcgcttccg gcagccagga ttgtttgtct
     1141 ttgatgggcg accctttttt aaaaaaaaga atcgttccca taatcatcat gtgaatcaga
     1201 aaaggcgatg tttcgatgaa tatcccctgt tctttaccgg cctccagaat gcccgccagg
     1261 atgctcataa ccgaggcaat atcctcgatg acaagtcgcg gaagcgttgc gccgccggaa
     1321 gcgatttcgc gcatcatcat cggcggcaga tcgggattcg cctccacggc gccggcgatg
     1381 aaagtgatat aggccttcat tttttcttcc ggatgaactt tgcgcgaaac ggcgtcggcg
     1441 atgtcccggg cgacatgacc gatcacctga tggagcgcgc cggtataaag cgtgtccttg
     1501 tcgccgatct ggtaatagat ggttgctttg tttacattgg cgcgcgctgc aatttcatca
     1561 atgcgtgcgc cgttgtatcc cttttgggca aaaaccgttc tggctgctgc cataatgcgt
     1621 tctgatgttt tatctgattt atctttgcgc ttgcggtcca tatttttatg ttaaattaga
     1681 atattataaa atacaaagga ttgcggtata ttttaaacta aacagttatt ttaactattt
     1741 atttaaatta aagtaaaaaa tccttttttt gtcaagagat tttcttgtgt gagaagagaa
     1801 atgccccgtg attgcgcaat cacggggcat tttgtgttga atttctgaaa gctttggaaa
     1861 ctattttgta accatttttt ctataaccgc ttcaacccgg cgattcttca tacgtccctc
     1921 aggtgtatta ttgtcggcga tcggcctgga gggtccgtaa cccacagcgg tgatgcggga
     1981 ctcatcgatg ccgaatttat cgatcaaata tttgcggaca ctgtcggccc gtgcctggga
     2041 gagttttata ttgttttcgg gattattgaa acggtcgacg ttatccgtat ggccttcgat
     2101 aacaacgttg gtatccggat acgtctgcat gaaatccgcg acttgcttga tgttattgtg
     2161 atactttttc ttgatgaacg attttgcagt atcaaattca atattgaggg aaatggagac
     2221 cttctcctgc accggcgggg gaggaggagg cggcgctatc gtttccggcg gcggaggtgg
     2281 aggcagcggc ggcacttctt cctgcttcgg ttcggctaca gtcatcttgc cttccttgcc
     2341 gaaacgcacg ggcagtaccc tgtttctgct tttgtggttg acggactcgg gaaaccacat
     2401 ttgacggtct ttgtcgaaac cgaccaccag ggcttccgaa ccctgaacgt cggatgtcca
     2461 tggatacgag ccgctcaggg taatcagttt tgtcagacga tacccgttat tgccgaagat
     2521 agaccggtcg tataaggtca gcaattcatc ttttgtcggc aggcgccatc ccttgtaacc
     2581 gctgccgtca tacgtcttgc aatattcctc cgcatccgac cagtttatat tgcttccgtt
     2641 atcgtgggag gcccataaca aattgctctg cgaatcccat actgtgccgt catcgtaggc
     2701 gatgaaacgg ccgtcgcgcg cttcttctgc agcattggcg ttaaaaacac cggccagaat
     2761 aacgaccgcc agaatagtaa aaattttccc taatgtttta ctcattagcc gttaactcct
     2821 cctctgttaa tttgttttgt tctgagacga gttatttttt ttgtgatgtc gcaataattt
     2881 aattgcctga aatattgcat aattttcaga attcgtcaat tacggaaaca gcaccgcttt
     2941 tttcttcagt tcacgaaaga aaatcttctc cggcaggcca tccggttatt ttcatgccgg
     3001 caacgtcatt cctcacgggg gcttcatttt ttcatcagcg cttgtatctg cttgtccttt
     3061 tcacgccaga gtttctgcac ccatttgtgc atggaatcac gaaacgcctg atcattttca
     3121 taatcgccct gcaaaagggt cttcgg
//
LOCUS       DAOSME010000157         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_33793_length_3129_cov_19.832791, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000157 DAOSME010000000
VERSION     DAOSME010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33793_length_3129_cov_19.832791"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2328
                     /gene="fdnG"
                     /locus_tag="PLG98_11690"
     CDS             <1..2328
                     /gene="fdnG"
                     /locus_tag="PLG98_11690"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOE33971.1"
                     /translation="YAPLRSGTDIAFLGGMIKYILDNKLYFAEYVKNYTNASFLVNPD
                     LKLPGENNGVFSGLTDGKYDKGTWSYQKGPDDVIKKDPMLSDPHCVFQLLKKHYSRYT
                     PDVVSRITGTPKDKLLEVYKLYASTGKPNKAGVDLYAMGWTQHTVGTQNIRTMCMIQL
                     LLGNIGIAGGGVAAMRGESNVQGSTDHGLLFHIWPGYLGTPTGSLATLKDFIEKRTPK
                     TKEKDSLNWWKNTPKYVAGFLRSMYGSDMTLEDAYEALPKLDDGANYSWLTIFDQMYK
                     GKFTGFFAWGMNPACSGAHSNKVRQALGKVDWMVNVNLFDNETGSFWRGPGMDPSKIK
                     TEVFMLPCASSVEKEGSIANSGRLQQWRYKAIPYYGDSKPDGDIMSEIFFKVKKLYEK
                     QGGPNAAAITKMTWPYGKHEGKEFHYEPRKVAAEINGFFLEDKTVENPTKKGEFKSFK
                     KGDLVPTFAWLQDDGSTSSGCWIYCGSVSEKGILPMRRGQADPTGLGLYSEWAWSWPV
                     NRRIIYNGASVDLNGKPWDPSRAVITWDAKEEKWKGDVPDGAGNPGKGRPPFIMKPDG
                     VASLYGPGLADGPFPEHYEPLECPVEKNLLSPQKNNPAIKRFDKEGVGSDMDVYSGVG
                     TCDPRFPFICSTYRVSEHWQTGVLTRWCPWLAEMQPGMFVEMSQELAKMRGIETGDKC
                     TVSTARGKVVCTAIVTPRFKPFDIDGNEIHEIGIPWHFGWITTKDRPFNKGDKVAEVF
                     TTGDAANLLTPTIGDANTMIPESKAFMANVVKGVK"
     gene            2330..>3129
                     /locus_tag="PLG98_11695"
     CDS             2330..>3129
                     /locus_tag="PLG98_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE33972.1"
                     /translation="MTEKIKLYDASKCTACRGCQLACKQWNGLKAGQTEHTGTYQNPP
                     SLQHNTYMIIHFQDYVAPEGDVRWIFRKEACMHCTNAACVTVCPSGALYYNKEFGTVG
                     MDREKCIGCKECISACPFEVPQYDKKTEKVFKCDMCETRITNNLTPACVKACPTGALK
                     WLDKEEAVKTAEARVQQLGGDANVYGDKFVGGTHMMYVLKEKPEVYEGIKQDPKVPFS
                     LTVWKSWLKPLSLLGAGGVIGGALLHYILHGPHKPHPEHYDQDTKQGGA"
BASE COUNT          799 a          748 c          862 g          720 t
ORIGIN      
        1 tacgcaccgc ttcgttcagg aacggatatc gctttcctgg gcggcatgat caaatatatt
       61 ctggataata aactttattt tgctgaatac gtcaaaaact acaccaacgc ttctttcctg
      121 gtcaatcccg atctgaaact tcccggagag aacaacgggg tattttccgg tttgacagac
      181 ggcaaatatg ataagggtac ctggtcttat cagaaaggcc cggatgatgt gatcaagaaa
      241 gatcctatgc tgtcggatcc ccactgcgta ttccagcttt tgaaaaaaca ttattcccgt
      301 tacacgccgg atgttgtttc caggattacg ggaacaccca aagataaact tcttgaagtt
      361 tacaaactgt acgcttcaac cggcaaaccc aataaggcgg gcgttgatct ttatgccatg
      421 gggtggacgc agcacacggt cggcacgcaa aacatccgga ccatgtgtat gatccagctt
      481 ttgttgggca acatcggtat cgcgggcggc ggggtcgcgg ccatgcgcgg cgagtccaac
      541 gttcagggtt ctaccgacca cggtctgctt ttccacatct ggccgggcta tctgggaacg
      601 cccaccggtt cactggcaac tctgaaagat tttattgaga aacggactcc gaaaaccaag
      661 gaaaaagatt ctttgaactg gtggaagaac acacccaagt atgtcgccgg tttccttcgc
      721 tccatgtatg gatcggatat gactctcgaa gatgcctatg aagccctgcc caaacttgat
      781 gacggcgcca actactcgtg gctgacgatt ttcgatcaga tgtacaaagg caagttcacc
      841 ggcttctttg cctggggcat gaatcctgcc tgttcaggcg ctcattccaa taaggttcgt
      901 caggcattgg gcaaagtgga ctggatggtg aatgtcaacc tgttcgacaa tgaaaccggt
      961 tctttctggc gcggtcccgg aatggatccg tcaaaaataa aaacagaagt tttcatgctg
     1021 ccgtgcgcgt cttcggtgga gaaagagggc agtatcgcca acagcggacg tctgcagcag
     1081 tggcgttata aagccattcc gtattacggc gattccaagc cggatggcga catcatgagc
     1141 gaaatctttt ttaaggtcaa aaaactttat gaaaagcagg gcggtcctaa tgctgcggcc
     1201 atcactaaaa tgacgtggcc ctacggcaag catgagggca aggaattcca ctatgaaccc
     1261 cgtaaagtgg ccgcggaaat caacggtttc ttccttgaag acaaaactgt tgagaatccg
     1321 accaagaagg gtgagttcaa gtcctttaag aaaggcgatc tggttccgac gttcgcctgg
     1381 ctgcaggatg acggttccac ttcatcgggc tgctggatct attgcggctc cgtgagtgaa
     1441 aaagggattc tgcccatgcg tcgcggtcag gccgatccga caggtctggg actttattcc
     1501 gaatgggcct ggtcctggcc ggtcaatcgc cgcatcattt ataatggcgc gtcagttgat
     1561 ttgaacggca aaccctggga tcccagccgc gctgtgatta cttgggatgc caaagaagaa
     1621 aaatggaagg gcgatgttcc cgatggagcc ggcaatcccg gcaaggggcg tccgccgttt
     1681 atcatgaaac cggacggcgt ggcctctctg tatggtcccg gtttggccga cggtccgttc
     1741 cccgagcatt acgaaccgct ggaatgcccg gttgagaaga atctgctttc accgcagaag
     1801 aacaatccgg cgatcaagcg ttttgataaa gaaggtgtcg gctccgacat ggatgtgtat
     1861 tccggcgtcg gaacctgcga tccccgcttc ccgtttattt gttccaccta tcgggtcagt
     1921 gaacattggc agaccggtgt tttaacccgc tggtgtccgt ggctggcgga aatgcagccg
     1981 ggcatgtttg tggaaatgag tcaggaactg gcgaagatga gaggtattga aaccggagat
     2041 aaatgtacgg taagtacagc gcgcggcaaa gtggtctgta cggccattgt gacaccccga
     2101 ttcaagcctt ttgatattga cgggaatgaa atccatgaaa tcggtattcc ctggcatttc
     2161 ggttggatta cgacgaaaga ccgtcccttc aataaagggg ataaggttgc ggaggtattc
     2221 accaccggtg acgcggcgaa tctcttgacg ccgacaatcg gtgatgccaa cacgatgata
     2281 ccggagagca aagccttcat ggcgaatgtt gtaaaggggg tgaagtaaaa tgactgagaa
     2341 gattaaatta tacgatgctt cgaaatgtac agcgtgccgc ggttgccagc tggcctgcaa
     2401 gcagtggaat ggattaaagg ccggacagac ggaacatacg ggcacgtatc agaatccgcc
     2461 ttctttgcag cacaatacgt atatgattat tcactttcag gattatgtcg caccagaagg
     2521 cgatgtcaga tggattttcc ggaaggaagc ctgcatgcat tgtaccaatg ccgcctgcgt
     2581 gacggtatgt cccagcgggg ctctttacta taataaagaa ttcggtacgg tgggtatgga
     2641 ccgggaaaaa tgcatcgggt gcaaggaatg tatttccgcc tgtccctttg aagtgcccca
     2701 gtacgataaa aagacagaga aagtttttaa atgcgatatg tgcgaaacaa gaattaccaa
     2761 caatttgact ccggcctgcg tcaaggcctg tccgaccggc gctctgaagt ggctggataa
     2821 ggaagaggcc gtcaaaactg ccgaagcccg cgtacagcaa ctgggcggcg acgccaatgt
     2881 atacggcgat aaattcgtag gcggcacaca tatgatgtat gtgttgaaag agaagccgga
     2941 agtttacgag ggtatcaaac aggatccgaa agttccgttc tccctgaccg tatggaagag
     3001 ctggctcaag cccttgagcc ttttgggagc cgggggcgtg atcggcggag cgcttctgca
     3061 ttacattctg cacggtccgc acaagcccca tccggagcat tacgatcaag atacgaagca
     3121 gggaggtgc
//
LOCUS       DAOSME010000158         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_34229_length_3099_cov_22.170499, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000158 DAOSME010000000
VERSION     DAOSME010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34229_length_3099_cov_22.170499"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..420)
                     /locus_tag="PLG98_11700"
     CDS             complement(154..420)
                     /locus_tag="PLG98_11700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOE33973.1"
                     /translation="MDSISLVAMASIIGAGLAMGIGSIGPGLGMGSAIAQALAAIAQQ
                     PDERNSLTRTLFVGLAMIESIAIYCFVISMILIFANPFWSHVIK"
     gene            complement(448..1179)
                     /locus_tag="PLG98_11705"
     CDS             complement(448..1179)
                     /locus_tag="PLG98_11705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOE33974.1"
                     /translation="MHIVQLSQISPDQVILWSWGFITLNVTILYTWLVMAILVVGSWL
                     VTRNLSSEINVSRWQHFLEVVISIIRGEISEMTKKGADHYIPLVGTLFLFICTSNVLA
                     VVPGYVAPTSSITTTAALATCVFIAVPFYGISRNGILHYLKEYFQPTFIFFPFHVMGE
                     LSRTLALTVRLFGNIMSHEKVIGILLAVTPLLFPVVMQILGLLIGVIQAYIFAILSMV
                     YIASALSAEEEHHAPAEIKQESVAA"
     gene            complement(1221..1532)
                     /locus_tag="PLG98_11710"
     CDS             complement(1221..1532)
                     /locus_tag="PLG98_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOE33975.1"
                     /translation="MTLSLDTKMILYLLASFTAGAALGAAYFTGLWWTVRRLSKVQHP
                     SKLLMGSFGVRIAMVLAGFFLIMHGGHWERLALAMLGFIVVRKILTFALGPQHVAETA
                     Y"
     gene            complement(1516..1869)
                     /locus_tag="PLG98_11715"
     CDS             complement(1516..1869)
                     /locus_tag="PLG98_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOE33976.1"
                     /translation="MVEIRRTSGNRRRQIADRFAEQVAKKEALRRRGIRHQGETVWFG
                     LGMFGIVGWAVAIPTLIGIALGLWIDRTWPSQYSWALMFLVAGVIVGCFNAAYWVKKV
                     RSKIIEDDGEDDVES"
     gene            complement(1954..2334)
                     /locus_tag="PLG98_11720"
     CDS             complement(1954..2334)
                     /locus_tag="PLG98_11720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOE33977.1"
                     /translation="MMLKILLPAEVMLTQEVNKIVAEAENGSFCLMPNHIDFVATLAP
                     GIFTYVPVNGSQELLAMDVGTLIKKGSDVLVSTRNAVRAPDLGKLKEVVVQQYDVLDE
                     REKMVRSAAARLEASLIRRFVELK"
     gene            complement(2324..>3099)
                     /locus_tag="PLG98_11725"
     CDS             complement(2324..>3099)
                     /locus_tag="PLG98_11725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOE33978.1"
                     /translation="VMVFGQMNEPPGARFRVGHSALTMAEYFRDDAKQDVLLMIDNIF
                     RFIQAGMEVSGLLGQLPSRLGYQPTLATELAELEERICNTKTGAITSIQAVYVPADDF
                     TDPSAVHTFGHLSATIALSRKRASEGLYPAIDLLQSGSKMLMPNIAGERHYKIAQEIR
                     KTLAIYEDLKDIIAMLGISELSREDQRTVFRARRLERFFTQPFFVTEQFTGTAGKMVS
                     REDTLKGCERILNDEFADYPERALYMIGSIDEAKISHDA"
BASE COUNT          816 a          860 c          732 g          691 t
ORIGIN      
        1 tcggcaaacc gtgcggcaat cttcgcttcc ctgtcatcca tggccttgat gatccgcccg
       61 tacaaaaaac gtttcagcag atacatgagc aacaggaagt taaagatctg acaaaagaaa
      121 acaaaccagt cgatatgcat ggatccatcc ccctcattta atgacatgac tccagaaagg
      181 attggcgaaa atcagaatca tggagataac aaaacagtaa atggcaatgg attcaatcat
      241 ggccaatccg acaaaaagtg ttctggtcag agagtttctt tcatccggct gctgtgcaat
      301 agccgccaga gcctgggcga tggctgatcc catacccaac ccagggccaa ttgaaccgat
      361 tcccatcgcc aaacccgcac cgataatcga ggccatcgcg accaaactta tactatccat
      421 aaaaactcct cctttataaa attattatca agcagcaacg ctttcttgtt ttatttcagc
      481 aggagcatga tgctcctctt cagcagacag cgccgaagcg atataaacca tggaaagaat
      541 ggcaaagatg taggcctgaa tgaccccgat gagcagtccc aggatctgca tgaccaccgg
      601 gaataaaagg ggagtaacgg ccagcagaat gcctatgact ttctcatggc tcataatgtt
      661 gccaaacagt cgaacggtca gcgccagtgt ccgggacagc tctcccatga cgtggaacgg
      721 gaagaagatg aatgtgggtt gaaaatattc cttcagataa tgaagaatgc cgttgcgcga
      781 aatcccgtaa aatggaacgg caataaaaac acacgtcgcc agcgccgcgg tcgtggtgat
      841 cgatgacgta ggggcaacat acccgggcac aacggccagc acgtttgacg tacagatgaa
      901 aagaaaaagg gtcccgacca gcgggatgta atgatccgcg ccttttttcg tcatctcgct
      961 gatttctccg cggatgatcg aaatcacaac ttccagaaaa tgctgccacc gggatacatt
     1021 aatctccgag gaaagattgc gcgttaccag ccatgagccg accaccagga tcgccatgac
     1081 caaccaggta tataaaatgg tcacattgag cgtgataaac ccccagctcc aaaggatgac
     1141 ctgatcagga ctgatctgac taagttgtac aatatgcata tggaatcctc atccggcgac
     1201 ccggggctgc gcccggattt ttaatatgcc gtttccgcaa catgctgcgg ccccaaggca
     1261 aaggttaaaa tctttcggac aacgataaat cccagcatgg caagagccag ccgttcccag
     1321 tgtccgccat gcatgatcag aaaaaatccg gccagtacca ttgcaattcg aacaccaaaa
     1381 ctccccatga gcagctttga cggatgttgg acctttgaaa gcctgcgcac ggtccaccaa
     1441 agcccggtaa aataggccgc cccgagcgcc gcacccgcgg tgaacgatgc caataaataa
     1501 aggatcatct tcgtatcaag actcaacgtc atcttctcca tcatcctcaa taattttact
     1561 gcgtactttt ttcacccagt aagcggcgtt gaaacatccc acaatgaccc cggccacgag
     1621 aaacatcagc gcccatgaat actgactggg ccaggtgcga tcaatccata agcccagcgc
     1681 aataccgatc agcgtgggaa tggccaccgc ccagccgaca atgccgaaca tgcccagacc
     1741 aaaccatacc gtttcgccct gatgcctgat gcctcggcgt cgcagcgctt cttttttggc
     1801 cacctgctcg gcaaaacgat ccgctatttg ccggcgccgg ttgccggatg ttcgtctgat
     1861 ttcaaccatt tttaacccct tgttctatgt cctacgttct gcgtttctcc cgcgttaact
     1921 ttgaactttg aacattgaac gtagaacgtt tttttatttc aactccacaa accgtctgat
     1981 taaacttgcc tccagtcttg ccgccgcgga tctgaccatt ttctcccgtt catccaggac
     2041 gtcatattgc tggacgacga cttctttgag ttttcccaga tccggcgcgc ggacggcgtt
     2101 gcgtgtggat accagcacat ccgatccttt ttttatcagg gtgcccacat ccatcgccag
     2161 cagttcctgg ctgccgttga ccggcacata ggtaaagata ccgggcgcaa gagttgccac
     2221 aaaatcaata tgattcggca tcagacagaa ggaaccgttc tccgcctcgg cgacaatttt
     2281 attgacttcc tgcgtcagca tgacttccgc cggcaataaa attttaagca tcatgggaaa
     2341 ttttcgcctc gtcaattgag ccaatcatgt aaagtgcgcg ttccggataa tcggcaaatt
     2401 catcattgag tatccgttca cagcctttta aagtatcctc tctcgacacc atcttgcccg
     2461 ctgttcccgt aaactgctcc gtcacaaaaa acggctgggt aaagaatctt tccagacgcc
     2521 gggcgcggaa cacggtgcgc tgatcctcac gggacagttc ggaaattccc agcatggcga
     2581 tgatatcctt caggtcctca taaatcgcca gcgttttgcg gatttcctga gcgatcttgt
     2641 aatgacgttc cccggcaatg ttgggcatca gcatcttgga gccggactgc agcagatcaa
     2701 tggccggata caacccttca ctggcgcgct ttcgtgaaag agcaatggtt gcggaaagat
     2761 ggccgaaggt atgcacggcg gatggatcgg taaaatcatc cgctggaaca taaacggctt
     2821 gaatggacgt gatggcgccg gtcttggtgt tacagatgcg ttcttccagc tcggccagtt
     2881 ccgtggccag cgtcggctga tagccgagac gcgaaggcag ctgccccagc agaccggaca
     2941 cttccatacc ggcctgaatg aaacggaaga tgttgtcgat catcaggagc acatcctgct
     3001 tggcgtcgtc gcggaaatat tccgccatgg tcagcgccga gtgtccgacg cggaaacgcg
     3061 cgcccggcgg ttcgttcatc tgaccgaaga ccatcacgg
//
LOCUS       DAOSME010000159         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_34249_length_3098_cov_21.479790, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000159 DAOSME010000000
VERSION     DAOSME010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34249_length_3098_cov_21.479790"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLG98_11730"
     CDS             <1..210
                     /locus_tag="PLG98_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOE33979.1"
                     /translation="VDIDELVEGDLVFFHTNRSFGHVGIYIGNNEFIHASSRNRRVRI
                     DSLDHPFYQERFQRAVRIKELPVGG"
     gene            414..2345
                     /locus_tag="PLG98_11735"
     CDS             414..2345
                     /locus_tag="PLG98_11735"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOE33980.1"
                     /translation="MAQNLVEKILSKHLVSGTLIPGEEIGIRIDQTLTQDATGTMAYL
                     QFESMNMPKVKTELSVSYVDHNTIQIGFENADDQLYLQTVAQKYGMVFSRAGNGICHQ
                     IHLERFGKPGKTLIGSDSHTPTCGALSMIAIGAGGLDVALAMAGKPFYLVCPKVIKIK
                     LTGKLKPWISAKDVILKVLEIFSTKGNVNTVFEYGGKGVESLTIPERATIANMGAECG
                     VTTSVFPSDKMTRLFLRSQHREKDFVELKPDKDAKYVKVVNIDLSKIEPLTAKPHSPD
                     NISTVKKMQKIDVHQICLGSCTNSSYKDLVTVAKILKGKTVHPNVSFILAPGSRQVLK
                     ELSKNGYLTDLISSGARLMENACGFCIGNSQSPPSAAVSLRTSNRNFQGRSGTMDANV
                     YLVSPETAAAAVITGKFTDPRDLKMRYPKVTLPKKFSIDDTMIIRPDKKKKEIEVIRG
                     PNIGILPRSPELSDTIHGPVTIKVGDKITTDHIIPAGARMKYRSNIPKYSEFVFENVD
                     ATFAKRAMSIRQDGKQNIIVAGHSYGQGSSREHAALCPMYLGVRVVIAKSFERIHTAN
                     LINFGIIPLTFCSESDYDIIEMEDLLEITETKETIAGDGKFLMRNKTKGTAFYVTCDL
                     SERQKQIILAGGALNINNA"
     gene            2488..>3098
                     /locus_tag="PLG98_11740"
     CDS             2488..>3098
                     /locus_tag="PLG98_11740"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOE33981.1"
                     /translation="MAAKIKVKTTVVEMDGDEMTRVMWQMVKDKLLFPYLDMDIDYYD
                     LHVKHRDDTDDKVTVDAAKAIMKYGVGIKCATITPNEDRVKEYRLKKAWKSPNGTIRA
                     MLDGTVFRKPILVNNIKPNVSSWKKPIIIGRHAYGDVYSNTEFDVPSAGKLELVFTPQ
                     DGGKAVNVPVFDFPGKGIAQVNYNLEQSILSFARACFTYALSE"
BASE COUNT          878 a          766 c          725 g          729 t
ORIGIN      
        1 gtggatatcg acgaactggt cgaaggagat ctggtattct ttcatacgaa tcgttcgttc
       61 ggacatgtcg gaatttacat aggaaataat gaattcatcc atgcctcttc aagaaataga
      121 cgcgtccgga ttgactcgtt ggatcatccc ttttatcagg aaagatttca acgcgccgtc
      181 aggataaaag agttgccggt tggaggctga aaatctggat aaccttttgt ttttaaatgg
      241 gtttatccat cattcatttc cataaataac ggggctttgt aaaacgattg caaagccctt
      301 tattttttta aaatttctta ccttttgaca gttgattatc ggttaagatt attatataag
      361 ttcgcggctg taaatgcttt agaacattaa ttcttctctt caaggaataa aaaatggcac
      421 aaaacctggt agaaaaaatt ctgtcgaagc atttggtctc gggtactctt atccccgggg
      481 aagaaatcgg aattcgtatt gatcagaccc tgactcagga tgccacaggc accatggcgt
      541 atctgcagtt tgaatccatg aatatgccca aggtaaaaac ggagttgtcc gtcagttacg
      601 ttgatcacaa taccattcag atcggctttg aaaacgcgga cgatcaactg tatctgcaga
      661 ctgtagcgca gaaatacggt atggtgttct cccgtgcggg caacggcatc tgtcatcaga
      721 tccatctgga acggttcggc aaacccggaa agaccctgat cgggtcggac agccacacgc
      781 ccacctgcgg ggcactctcc atgatcgcca tcggcgccgg aggactggat gtggcgctgg
      841 cgatggccgg aaagccgttt tatctggtct gcccgaaagt gattaaaata aaactcaccg
      901 gcaagctcaa accctggatt tccgcaaagg acgttattct caaggtgctg gaaatctttt
      961 ctacaaaagg caacgttaac acggtatttg agtacggcgg caagggcgtt gagtctttga
     1021 ccatccccga gcgcgcaacc atcgccaaca tgggggccga atgcggcgta acaacatccg
     1081 tctttcccag cgataaaatg acgcgcctct tcctgcgatc acagcacaga gaaaaagact
     1141 ttgtcgagtt gaaacccgac aaagacgcta aatacgttaa agtcgttaat atcgatttat
     1201 ccaaaataga accgttaaca gccaaacccc atagtccgga caatatcagc acggttaaaa
     1261 aaatgcagaa gatcgacgtc catcagattt gcctgggaag ctgcaccaac tcgtcttata
     1321 aagatctggt taccgtggca aaaattttaa agggcaaaac cgtgcatccc aatgtcagct
     1381 ttattttggc tcccggttcg cgtcaggtgc tcaaggagct gtccaaaaac ggttacctga
     1441 cggatctgat ctcctcggga gcacgcctta tggaaaatgc ctgcggattc tgcatcggca
     1501 acagccagag ccccccgtca gccgccgtgt cgttgcgcac gtccaaccgt aattttcagg
     1561 gacgctccgg cacgatggat gccaacgttt atctggtcag cccggaaact gccgcagcgg
     1621 cggttataac cgggaaattc acggatccgc gcgatctgaa gatgcgctat ccgaaagtca
     1681 cgctgccgaa gaagttttcc attgatgaca caatgattat tcgtccggac aaaaaaaaga
     1741 aggagatcga ggtcatccgc ggtccgaaca tcggcattct gccgcgcagt ccggaacttt
     1801 ccgataccat tcacggaccg gtaaccatca aggtcggtga caaaattacg acagaccaca
     1861 tcattccggc cggtgcgcgg atgaaatacc gttcgaatat tccgaaatat tcggaatttg
     1921 ttttcgaaaa tgtcgatgcg acattcgcca agcgggccat gtccatcagg caggacggga
     1981 aacaaaatat catcgtcgcc ggtcattctt acggccaggg atcttcccgc gaacatgccg
     2041 cgctgtgccc gatgtatctg ggagtacggg tcgtcattgc caaatctttt gaaagaattc
     2101 acacggccaa tctgatcaac ttcggcatca taccgctgac attctgttca gagagcgatt
     2161 acgacatcat tgagatggaa gatctccttg aaattactga aacaaaagag accattgccg
     2221 gggacggaaa atttctaatg agaaacaaaa caaaggggac cgctttttat gtcacctgcg
     2281 atttgtccga aaggcagaaa cagatcattc tggccggagg agctctgaat atcaataatg
     2341 cataaggtcc cgcggcagac tgcggaagtt cattcaaccc aaaacttcaa tacgccaggc
     2401 tgttgaagtt cacatttcat tataataagc aagcggcagc gcactttgtt ctgaagcttg
     2461 ggtctcatga aggagcagta tttttttatg gcagcaaaaa ttaaagtgaa aaccaccgtc
     2521 gtcgaaatgg acggcgatga aatgacccgc gtgatgtggc aaatggtaaa ggacaaactc
     2581 cttttcccct atctggacat ggatatcgac tattatgatc tgcatgtaaa acaccgcgac
     2641 gatacagacg acaaagtcac cgtcgatgca gccaaagcga ttatgaaata cggcgtcggc
     2701 atcaaatgcg caaccatcac ccccaacgaa gatcgtgtca aagaataccg cctgaaaaaa
     2761 gcatggaaaa gccccaacgg caccatccgc gccatgctgg acggtacggt tttccggaaa
     2821 cccattctgg tcaacaatat caaaccgaat gtttcctcct ggaaaaaacc catcatcatc
     2881 ggccgtcacg cctacggcga tgtatacagc aatacggaat ttgatgttcc gtccgccggc
     2941 aaacttgaac tggtctttac gccgcaggac ggcggcaagg ccgtcaacgt tccggtattt
     3001 gactttcccg gcaaaggcat tgcccaagta aactataatc tggaacaatc catcttgagt
     3061 tttgcccggg cctgtttcac ctacgccctc agcgaaaa
//
LOCUS       DAOSME010000160         3069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_34670_length_3069_cov_18.959190, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000160 DAOSME010000000
VERSION     DAOSME010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34670_length_3069_cov_18.959190"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..594
                     /gene="ectA"
                     /locus_tag="PLG98_11745"
     CDS             61..594
                     /gene="ectA"
                     /locus_tag="PLG98_11745"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HOE33982.1"
                     /translation="MKNDSHHILFRLPEKKDAQAVRRLIEDSPPLDLNSLYCYLLLCT
                     HFAPTSAIAEYHQEVCGFVSAYIRPDQKDTLFVWQVAVQRDFRGKSIAKGMLLSCLQR
                     AHLSSLRYLETTVNPSNSSSNALFSSLAEFLNAGLAKSALFSADDFGKTSHEEEILYR
                     IGPFNISKEGESINENN"
     gene            581..1834
                     /gene="ectB"
                     /locus_tag="PLG98_11750"
     CDS             581..1834
                     /gene="ectB"
                     /locus_tag="PLG98_11750"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR00709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HOE33983.1"
                     /translation="MKTIRRLESEVRSYSRSFPVLFEKAKDAFLFDAGGTRYIDFFAG
                     AGTINYGHNNEYMKRKIFDYLENDGIVHGLDMATVAKEELLLKMEATLFKPKNLDYKV
                     QFTGPTGTNAVEAALKLARMVKKRSNIISFTNGYHGLTMGSMSVTANAYYRDEAFINR
                     SDVSFMPYDGYLGKHVNTAKYLRRFLEDNSSGVDIPSAIILETVQGEGGIRVAGDEWL
                     REVEKICRDFDILLIVDDIQVGNGRTGNFFSFEKSGIKPDIVTLSKSFSGFGLPMSVV
                     LFRRDLDIWKPGQHTGTFRGNNLGFICASVALDYWKDDRFSKEIFRKGKILHKRLNKI
                     SAKYPQLHTEVRGRGFVYGMGLSTGEIAKKILTECFHNKLILESAGARNNVIKFLAPL
                     TITDEVLEEGLDILERSIDNVMKKH"
     gene            1846..2235
                     /locus_tag="PLG98_11755"
     CDS             1846..2235
                     /locus_tag="PLG98_11755"
                     /inference="COORDINATES: protein motif:HMM:NF009806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HOE33984.1"
                     /translation="MLVRTIDDIRGTRREVFAENKNWVSRRLLLKKDGMGFSFHETII
                     FAGTETHIWYKHHLEAVFCVEGEGEVETVADGKIHRITPGTLYALNDHDEHYLRAQKD
                     MRLLCVFNPPVTGEEVHLADGSYPAPE"
     gene            complement(2272..>3069)
                     /locus_tag="PLG98_11760"
     CDS             complement(2272..>3069)
                     /locus_tag="PLG98_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOE33985.1"
                     /translation="DGVNDAPALKQANIGIAMGITGTDVSKEAADMVLTDDNFASITR
                     AVEEGRRIFDNLVKFITWTLPTNGGEGLVILAAIFSGVTLPILPVQILWINMTTAILL
                     GLMLAFEPGEPDVMDYPPRAPQSPILSWTLISRICLVIALMLAAVFGIFIWQKQQGYS
                     VEAARTVAVNLFIMIELAYLFNCRSLTKSMFTIGVFSNPWVLFGSAAMIVLQLLYTYA
                     PFMNRIFQSEPISGRDWLLIIAVSLAVHIVIGAEKWIRHHVFKQSHA"
BASE COUNT          781 a          794 c          793 g          701 t
ORIGIN      
        1 attaacctcg tcgttcaata acagcggcaa accaaaaata ataaacatca ggagacggat
       61 ttgaagaatg attcccatca tattttattt cgcttgcctg aaaaaaaaga cgcgcaggcg
      121 gtaaggcggc ttatcgaaga ttctccccct ctggatctga attcgctgta ctgttacctt
      181 ctgctctgca cgcatttcgc gccgacatcg gcaattgccg agtatcatca ggaggtttgc
      241 ggattcgttt ccgcttatat ccgcccggat caaaaagata ccctgtttgt ctggcaggtg
      301 gcggtccaaa gggatttccg gggaaaatcc atcgctaaag gcatgttgtt atcgtgtctg
      361 cagcgcgctc atttatcttc cctgcgttat ctggaaacga cggttaatcc gtccaactca
      421 tcatcgaacg ccctgttttc ctcgctggcc gaatttctaa atgccggtct ggccaaaagc
      481 gctttgtttt ccgcggatga tttcggaaaa acgtctcatg aggaggaaat tctgtatcgt
      541 atcgggccgt tcaacatttc taaggaagga gaatctataa atgaaaacaa ttagacgact
      601 ggaatccgag gttcgcagtt attcgagatc tttccccgtt ttgttcgaaa aggccaagga
      661 cgcctttttg tttgacgcag gcggaacccg ttatatcgat tttttcgcag gcgcggggac
      721 gatcaactac ggacataaca acgaatacat gaaaaggaaa attttcgatt atctggaaaa
      781 cgacggcatc gttcacggtc tggacatggc cacggttgcc aaggaggagc tgctcctcaa
      841 aatggaggcc accctcttca aaccgaaaaa tcttgattac aaggtgcagt tcacgggtcc
      901 cacgggaacg aacgccgtgg aggcagcgct caaactggcc cgcatggtaa agaaacgctc
      961 caacattatc tcttttacca acgggtatca cggtctgacg atgggctcga tgtccgtgac
     1021 cgccaacgcc tactaccgcg acgaggcgtt catcaaccgt tccgatgttt cctttatgcc
     1081 ctacgacgga tatctgggta aacacgtcaa caccgccaag tatctgcggc ggtttctgga
     1141 agataacagc agcggggtcg atattccttc ggccatcatt ctggaaacgg ttcagggtga
     1201 aggcggcatt cgcgtggcgg gggatgaatg gctgcgcgag gtggaaaaaa tctgccggga
     1261 cttcgatatc ctgctgattg tggacgacat tcaggtcggc aacggacgga ccggcaattt
     1321 tttcagcttt gaaaagtccg gcattaaacc ggacattgtt acgctctcca aatcgttcag
     1381 cggtttcggc ctgccgatgt ccgttgtgct gtttcgccgc gatctcgata tctggaagcc
     1441 cggccagcat accggaacgt tccgcggcaa caatcttggc tttatctgcg cgtccgtggc
     1501 gctggattac tggaaagacg accgcttctc caaagagatc ttccgcaaag ggaaaattct
     1561 gcacaagcgg ctcaataaaa tcagcgcgaa atatccgcag cttcacacgg aggtgcgcgg
     1621 gcggggtttt gtctacggaa tgggcctgtc caccggcgag atcgccaaga agattctgac
     1681 cgaatgtttc cacaacaaac tgattctgga atcggccggc gccagaaaca acgtcatcaa
     1741 atttcttgct cctctgacga tcacggatga agtgctggaa gaaggcctgg atattctgga
     1801 gcgcagcatc gacaacgtta tgaaaaaaca ctgaaaggat ttctcatgct cgtaagaaca
     1861 attgacgata tccggggaac ccgccgggag gtattcgccg aaaataaaaa ctgggtcagc
     1921 cgcagactgc ttttaaaaaa agacggcatg ggattttcgt ttcatgaaac gatcatcttt
     1981 gccggcaccg aaacgcacat ctggtacaag catcatctgg aagcggtttt ttgcgtagag
     2041 ggagaaggcg aggtcgaaac ggttgcggac ggaaaaatcc accgcatcac acccggcaca
     2101 ttatacgccc tgaacgacca cgacgaacat tatctgcggg cccaaaagga tatgcgcctg
     2161 ttgtgcgttt tcaacccgcc ggtcaccggg gaagaagttc atctggcgga cggatcctat
     2221 cctgcgccgg aatagtggaa cgtcctgccc cctgtctgca gccggaattc tttatgcgtg
     2281 cgactgctta aaaacgtgat gccggatcca tttttccgca ccgatcacga tatgaacggc
     2341 cagggaaaca gcgatgatca acagccagtc cctgccggaa atgggctcgc tctggaaaat
     2401 tctgttcatg aacggcgcat aggtgtagag caattgcaga acaatcatcg ccgcactgcc
     2461 gaataaaacc cacgggttcg agaaaacacc gattgtgaac attgacttgg tcagagagcg
     2521 gcagttgaac aaataggcca gttcgatcat gatgaaaaga ttcaccgcca cggtgcgcgc
     2581 ggcctctacg gaatagccct gctgtttctg ccagataaaa atgccgaata ccgccgccag
     2641 catcagggca atcaccaggc atattctcga tataagcgtc cagctcagga tggggctttg
     2701 gggtgcccgc ggagggtaat ccataacatc cggttcgccg ggctcaaagg ccagcatcag
     2761 acccagcaga atcgcggtcg tcatgttaat ccagagaatc tgtacaggta atatgggcag
     2821 ggtgacgccg ctgaaaatgg ccgccaggat caccaggcct tcaccgccgt ttgtcggcaa
     2881 tgtccacgtg ataaatttta caagattatc aaaaattcga cggccttcct ccaccgcccg
     2941 cgtgatgctg gcaaaattat catcggtgag caccatatcg gccgcttctt tggacacatc
     3001 cgttccggta atgcccatgg caatgccgat gttggcctgt ttcagcgcgg gcgcatcgtt
     3061 gaccccgtc
//
LOCUS       DAOSME010000161         3069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_34671_length_3069_cov_13.636032, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000161 DAOSME010000000
VERSION     DAOSME010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34671_length_3069_cov_13.636032"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLG98_11765"
     CDS             <1..418
                     /locus_tag="PLG98_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOE33986.1"
                     /translation="AISSPLLEDNTIQGAQGILLSITGGPDMSLYEVNEASSLIQAEA
                     HEDANIIFGTVIDENMKDEIRITVIATGFEDAKKKQVPSNVTHLGGFRREDLSTPAFM
                     RKSKSGDPANVVTMGVNDENENVDIDIPTFLRRQAD"
     gene            477..2165
                     /locus_tag="PLG98_11770"
     CDS             477..2165
                     /locus_tag="PLG98_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE33987.1"
                     /translation="MNWKEKKKYDALLARERGVMKKVWGTCLTVCLAYPNLYRIGMAN
                     LGFQTVYKAINDLPSLLCERVFLHAGDGAEYAAGAVEPLSLESRKPLRDFDILAFSVS
                     FENDYPAILKMLEWSGIPLKSKDRADGHPLVIGGGIALTLNPEPLADFFDIFVLGEAE
                     EILPQFARVFAEARRLELDRKDLFNDLQKQIPGIYVPSLYAVSYFPEGKIKDIAPRTE
                     GLPKKIQISPIRNINAFCTTEVVSGADAEMADMFLVEVNRGCPHLCRFCAAGFVYAPP
                     RFRGYEEIVAAVDRGLMIKNKIGLVGTAVSDHPDLIKICRYIVGRGAQAGIGSLRLDK
                     IDESIVDLLKTGGIETVALAPEAGSQRLRDLLRKGITTDDILNAAKLLIEKEMSNLRL
                     YFMVGLPSEEDSDIDAIIDLVKKIQHTALAQTKGKRKFRRITLSINQFIPKPRTPLQW
                     CPLADVQDVKAKIKKIIQAFRADKQINVIAGVAKWNYVQALLSSGDRRVGDILLAAHR
                     LNGNWMRALKEVTINPDFYVYRQKDVNEFLPWDIIEAGLPKKALIKEYQKALPD"
     gene            2218..2511
                     /locus_tag="PLG98_11775"
     CDS             2218..2511
                     /locus_tag="PLG98_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33988.1"
                     /translation="MKAFSVMPTEAGIDLILISEDFRNHDYWSRIDILSEAIYDVFVP
                     IEVVGFTPEEWDNQTSPLYDYAKQGEILYAARPCQCYDAFPYFSLRKFISEPV"
     gene            complement(2580..>3069)
                     /locus_tag="PLG98_11780"
     CDS             complement(2580..>3069)
                     /locus_tag="PLG98_11780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE33989.1"
                     /translation="VANVFLPGAGYLNHIINPTITNNYSKTQEYDADQMASDHCAQCF
                     DMSIEKQITVMQKIEKISKSSGGGFWATHPSWENRIKNVATTTKSSSQIRNIKTTAQS
                     PSPLMEGDQKHKEIKGIVLNDGNVVEGQLLEMSTDTVMIQTEDGKVSTYSFEKEVRGF
                     VK"
BASE COUNT          739 a          772 c          799 g          759 t
ORIGIN      
        1 ggcgatttcc tcgccgcttt tggaggacaa caccatccag ggcgcgcagg gcatcctgct
       61 cagcatcacc ggcggtcccg acatgagcct gtacgaagta aacgaggcct cgtccctgat
      121 tcaggccgag gcgcacgagg atgccaatat catcttcggc acggtcattg acgaaaacat
      181 gaaagatgag atccgcatta ccgtgatcgc aaccggcttc gaggacgcca aaaagaagca
      241 ggttccctcc aatgtgacgc atctgggcgg cttccggcgg gaggatctgt caacgccggc
      301 gtttatgcgc aagagcaaat ccggcgatcc ggccaatgtg gtaacgatgg gcgtcaacga
      361 cgaaaacgaa aacgtggata tcgacattcc cacgttcctg cgccggcagg cggactagga
      421 aaacatcgtg gctgtccgaa tgcgggctgg atcgcaaagg aggccatgaa ctgtccatga
      481 actggaagga aaagaaaaaa tacgatgccc tgctggctcg cgagcggggc gtgatgaaaa
      541 aagtctgggg aacctgcctc acggtttgcc tggcctatcc caatctttac cggatcggca
      601 tggccaacct gggcttccag acggtttata aagcaattaa cgatctcccc tcccttcttt
      661 gtgagagagt gtttctgcac gccggcgacg gagccgaata cgccgccggc gcggtggaac
      721 ctctcagcct ggaaagccgg aagccgcttc gcgattttga tattctggcg ttttccgtct
      781 cttttgaaaa cgattacccc gccatcctga aaatgctgga atggagcggc atcccgctga
      841 aatcgaagga ccgcgcggac gggcacccgc tggtgatcgg cggcggcatt gcgctgacgc
      901 tcaatccgga gcctctggcg gattttttcg atatctttgt tttaggtgaa gccgaggaaa
      961 tcctgccgca gtttgcgcgg gtgtttgctg aagcccggcg gcttgaactc gaccggaaag
     1021 acttgttcaa cgatttacaa aaacaaatac cgggaattta cgttccctcg ctctacgcgg
     1081 tgtcctattt tcccgaaggc aagattaagg atattgcgcc gcgcacggag ggactgccaa
     1141 agaaaattca gatttcaccc atcagaaata ttaatgcttt ttgcacgacc gaagttgtca
     1201 gcggcgccga tgcggaaatg gcggacatgt ttcttgtcga agtcaaccgc ggctgtcctc
     1261 acctctgccg gttttgcgcg gcaggttttg tttacgcgcc gccgcgcttc cgcggctatg
     1321 aagaaatcgt ggccgccgtc gatcgcggtc tcatgataaa aaataaaatc ggcctggtcg
     1381 gcacggcggt ttccgatcat cccgacctga taaaaatctg ccggtatatt gttggtcgcg
     1441 gagctcaggc gggcatcggc tcgttgcgcc tggacaagat tgatgaaagc attgtagatc
     1501 tgctcaagac cggcggcatc gagacggtcg ccctcgcgcc cgaggcggga agccagcggc
     1561 tgcgcgatct gctgcgcaag gggatcacaa cggacgatat cctgaacgcc gcgaagcttt
     1621 tgattgaaaa agaaatgtcc aacctgcgtc tgtactttat ggtgggcctg ccttcggaag
     1681 aagattccga tatcgacgcc atcatcgatc tggtcaaaaa aatccagcac acggcgctgg
     1741 cccaaacaaa aggaaaacgg aaattccggc gcatcacgct gagcattaat cagtttattc
     1801 ccaaaccgcg aacgccgctc caatggtgcc cgctggcgga cgtgcaggac gtgaaggcca
     1861 aaataaaaaa aatcattcag gcgttccgtg ccgacaaaca aatcaacgtg atcgccggtg
     1921 tggcgaagtg gaattatgtt caggcgctct tgtcttccgg ggatcgtcgt gtgggtgaca
     1981 ttctgctggc cgcgcatcgt ctaaacggca actggatgcg ggcgctcaaa gaggtgacca
     2041 tcaatcccga cttctacgtc tatcgtcaaa aggatgtaaa tgaattcctg ccgtgggaca
     2101 tcatcgaagc cggccttccc aaaaaagcgc tgatcaaaga atatcaaaag gctctacctg
     2161 attgatcttc cctccctcgt gcgcttttta gagtatgagg ggagggattg agggagggtg
     2221 aaagcctttt ccgtcatgcc gacggaggca ggcatcgatc tgatcctcat ttcagaagac
     2281 ttccggaacc atgattactg gtcaaggatt gatatactgt ccgaagccat ctatgatgtt
     2341 tttgtcccga ttgaggttgt cggtttcacg cctgaagagt gggacaacca aacatcaccg
     2401 ttatatgatt atgccaagca aggtgaaatt ttatacgcag cgcgaccatg tcaatgttat
     2461 gatgcttttc cttatttttc attgaggaag tttataagtg agccagttta atcaatgtca
     2521 ttagtgattt tctgaaaaga tacgaagagc cctgcccgtg tacccccgta ctttatctgt
     2581 tatttgacaa acccacgaac ttctttttca aatgaatagg ttgatacctt tccgtcctct
     2641 gtctgaatca tcacggtgtc cgtgctcatt tcaagtagtt gcccttcgac aacatttcca
     2701 tcatttaaca cgatgccttt tatttctttg tgtttctgat ctccttccat tagcggcgaa
     2761 ggagattgcg ctgtcgtttt gatgtttcta atctgtgaag aagacttcgt tgtcgttgct
     2821 acgtttttaa tccggttttc ccatgacgga tgtgtcgccc aaaaaccacc accacttgat
     2881 ttagatattt tttctatttt ttgcattacg gtgatctgtt tttcgatgga catatcaaag
     2941 cattgagcgc aatgatctga tgccatttga tccgcatcgt attcttgggt tttgctgtaa
     3001 ttgttagtaa tagttggatt aatgatatga ttcaaataac cagcgccggg aaggaaaaca
     3061 tttgccacg
//
LOCUS       DAOSME010000162         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_35112_length_3039_cov_25.205764, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000162 DAOSME010000000
VERSION     DAOSME010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35112_length_3039_cov_25.205764"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..950)
                     /locus_tag="PLG98_11785"
     CDS             complement(141..950)
                     /locus_tag="PLG98_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE33990.1"
                     /translation="MNDSYRAFYGLTKEPFGSEISLKEILETTDLKDVKNRFDYAVRL
                     GAVALVTGEIGSGKSTALRYAAGHLHPSAYCVFHVVATSGSIIELYRQILGEMGTDTA
                     SFSKAVLLKRIRREITEIVGNKKIHAVLVIDEASLLRMDVLAEIHTLCQFEQDSKPWL
                     PLIMAGQLNLVDKLSYRSSMPLASRVVAKAHLVSVNRQGMEAYLNHHLNIAGVKTPLF
                     DETAITAIHQGSGGLFRKANHLARGALIAAAHEQTSSVSANHVRLAATEIF"
     gene            complement(943..>1383)
                     /locus_tag="PLG98_11790"
     CDS             complement(943..>1383)
                     /locus_tag="PLG98_11790"
                     /inference="COORDINATES: protein motif:HMM:NF020859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HOE33991.1"
                     /translation="NEAFDAWLRDVYHIRKHSSTGQSPFERFTANMACLRTAQADLRD
                     HFRTVARRTVAKDRTLTINGKLYEAPVALIGKRVEVLYHENDPERVEVRFSKQSYGHL
                     VPVDIHVNCRVKRRDGKTIVETPETQNNYRGGMLWSNNREVNHE"
     assembly_gap    1384..1483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1484..2294)
                     /locus_tag="PLG98_11795"
     CDS             complement(<1484..2294)
                     /locus_tag="PLG98_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOE33992.1"
                     /translation="MTEEQKKRVAVFRFGVIHDLIDGNGVNQETQEELLRGKCARKWD
                     IPFSGRSRIGRSTILNWVRLYKDSDGKIESLYPADRSDRGKSRTMDEETTLALVNLRR
                     EMPKTTVPLLIGKMIERKLITPGTTLNLSGVYRLLNWHGLMKVKEHQTEDRRKFEAEL
                     PNDIWQSDVMHGPMVSVGDKTKKSYLIALLDDHSRLITHGEFYLSENIHSYVNALEQA
                     LLKRGLPRKLYLDNGPAFRSLHLEHITASLGIALIHSRPYKPQGRGKIERFF"
BASE COUNT          690 a          770 c          745 g          734 t
ORIGIN      
        1 tgttgatttt cacattaaat ggacgggtat tgtctgatta aatgggacgg atttcagttt
       61 tgttagatta tatggacggg aagcccgagg agggcctccc ttgtttgcat attttaaata
      121 tccgttatta tgttttgtag ttagaagatt tccgttgcag ccaggcggac atggttggcg
      181 gagacggaag atgtttgttc atgagcggcg gcgatgaggg cgcctctggc gaggtggttt
      241 gcttttcgga acaggccgcc ggatccctgg tggatggcgg tgatggcggt ttcgtcgaag
      301 agaggtgttt tgacgccggc gatgttcagg tggtgattca agtaagcttc cattccctgg
      361 cgattgacgc ttacgagatg agctttggct actactcgag aggcaagggg cattgatgat
      421 ctataggaga gtttatcgac gagattgagt tgtcctgcca tgatcagggg cagccatggt
      481 tttgagtcct gttcgaactg acagagggta tggatttcgg ccaggacatc catacgcagc
      541 agggatgctt cgtcgatcac caagacggcg tggatttttt tattgccgac tatttccgtg
      601 atttctctac ggatgcgttt gagcaggacg gccttggaga agctggcggt atcggtgccc
      661 atttctccca ggatttgtct gtacaactcg atgatggagc cggaggtggc gacgacatga
      721 aagacgcaat aggcggaggg gtgcagatgc ccggcggcgt atctcagggc ggtggatttg
      781 ccgctgccga tttccccggt taccagggcc acggctccca aacggacggc atagtcgaaa
      841 cgatttttga catctttcag atcagtggtt tccaggattt ccttgaggct tatctctgag
      901 ccgaagggtt cttttgtaag gccgtagaag gcgcgatagg agtcattcat gattgacctc
      961 ccggttgttg gaccacagca tcccgccgcg atagttgttt tgagtctccg gtgtttccac
     1021 gattgttttg ccgtcccggc gcttgacccg gcaattgacg tggatatcca ccgggacaag
     1081 atgcccgtat gattgtttgc tgaagcggac ctcgacccgt tccggatcat tttcatggta
     1141 caggacttcg acgcgtttgc cgattaatgc cacgggagct tcatagagct ttccattgat
     1201 ggtcagggtc cgatctttgg ccacagtgcg cctggctacg gtgcggaagt ggtcgcgaag
     1261 gtcagcctgc gctgttcgca ggcaggccat gttggcggta aaacgctcaa aagggctctg
     1321 cccggtggag ctgtgtttcc ggatatgata gacgtccctc agccacgcat caaaagcctc
     1381 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaaaaaa tctttcaatt
     1501 ttcccgcgac cttgtggctt gtaagggcgg gaatggatca gggcaatgcc cagagacgcc
     1561 gtaatatgct ccagatggag ggatcggaag gcggggccgt tgtccaggta tagcttcctg
     1621 ggcagtccac gcttcaaaag cgcttgctcc aaagcattta cataggaatg gatattctcg
     1681 gaaagataaa actccccatg ggtgatcaga cgggaatgat catcgagcag ggcaatcaga
     1741 taactttttt tcgtcttgtc tcccactgaa accatgggac catgcatcac gtcggactgc
     1801 cagatgtcat tgggcagttc cgcttcaaac ttacgacgat cctccgtttg atgttctttg
     1861 accttcatga ggccatgcca attcaataaa cggtaaaccc cggaaagatt cagcgttgtc
     1921 ccgggggtaa tcagcttacg ttcaatcatc ttgcctatga gcaagggtac cgtcgttttc
     1981 ggcatctccc ggcgcaagtt gaccagagcc agggttgttt cctcatccat ggtacgactc
     2041 tttccccggt cggaacggtc agcgggatac aatgattcaa tcttcccatc actatccttg
     2101 tataaccgca cccagttcaa gatcgtactg cgaccgatcc ggctcctccc tgaaaagggg
     2161 atgtcccatt tccgggcgca cttccccctg agcaactcct cttgagtctc ctgattgacc
     2221 ccgttcccgt cgatcaggtc gtgaatcacc ccgaaccgaa acaccgccac ccgctttttc
     2281 tgctcttccg tcacaaaaaa gcctcctttc cttttttcca gaggccttta taacaaccca
     2341 acgagcccgg cgatgacact cttcggtagg gtgtatcaaa accacccctt ctttcaggtt
     2401 atatgaaacg actcacggga atctgaccca gcgccataag acgatcgaat gcgcaaagaa
     2461 ggccgcctcg ccagcggttg tctaaatatg ccgccacacg cttcaccaat gcccgcaacc
     2521 agtgcctttg gcgattgcgc gtcatccccg gcggccatcg cccgctcaat aacctaaacg
     2581 acaaccgaga acgaatgtct tcaatagaag cctgaaatcg acgaaaatat ccacagggac
     2641 gaacacgaat cacacaacgg caatgaggac aacgccatct ccgcaaatat acaaaaccag
     2701 aaaaaccatc aaaatacgca gaaacaaaac cgtgacccca tacatgactc gcccggcaat
     2761 taggacatcg ctccggcttg ggccaaacat aatcctttcc cttttcaaaa atctccttga
     2821 ccgcgcagca aacgttgatt accatctggc cacctcatat gtgattccta tgtttcccta
     2881 tgagaaaggg cggtgcctct tcgaaaagtt caccgccctc ccaataataa aaaaacctct
     2941 ccaaagagag gtccatatat tctgacattc caactctact tgtcaatcca ttccgaaatt
     3001 atcaccccta tccttgctca tatattgtag aagcctaca
//
LOCUS       DAOSME010000163         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_35342_length_3024_cov_17.874368, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000163 DAOSME010000000
VERSION     DAOSME010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35342_length_3024_cov_17.874368"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLG98_11800"
     CDS             complement(<1..307)
                     /locus_tag="PLG98_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOE33993.1"
                     /translation="MPCDLHHVHLFASNLDGGIGFYREMFGAEIVFDEIVAGVRNVLV
                     RIGAGHINFYDQTPREDGKNAVHHLGILTDDIAALVAHRIAKGFTFRSPVRDFGDLKY
                     "
     gene            complement(321..923)
                     /locus_tag="PLG98_11805"
     CDS             complement(321..923)
                     /locus_tag="PLG98_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE33994.1"
                     /translation="MKQPKTFAHLKNGEKLARQTVIIQAAERVFAVKPFNKVNIRDIA
                     KEAGISHASIYRYFPDQQSLFIEAFLQGAKEITLTLEGLIEEAKIPDIIKISDAYTTY
                     LMDNDQYFRMMTHFMLDGSLSPEKIEKLNAAERQIFDQFDKLFRKMNCPEPVRLLSHA
                     FFAALNGVLITFRNYPGRTSKAVRRHMLNIARIIAGSFGK"
     gene            1049..2557
                     /locus_tag="PLG98_11810"
     CDS             1049..2557
                     /locus_tag="PLG98_11810"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOE33995.1"
                     /translation="MEINVGKMLANRAYLSPGLEAFSGIDYRFNFREANERANRFGAY
                     LKKSGLASGDRIAVLCKNNEHATTALFGAAKIGIITLMLNWRLQVPELDYILKDSGAS
                     LLLYDSEFAPVAEALKGQTAVNHFLNVEPGNRNNDFEKALAGVSAAEPPYAGFGADPA
                     VLMYTSGTTGRPKGVMLTHDNLCWASVGLAHTIDWAHKYRWLSVAPLFHIGGLSPIFG
                     NVHAGCTTFFVPAFDPVKIWDVIVDEKINFMMTVPAMLQFMLLAPDIAKKDLSSIRHI
                     VCGGSAVPQSLIETYDKMGIQVEQVYGITEYSGAVTFWIPDMGMEKSNSAGKTVFHGQ
                     VKILRPESDEEMAAGEVGEICCFGPQVFKGYWNNPDATKAAVGRGYYRSGDMGKIDAD
                     GFLYVVDRLKDMIISGSENIYPAELEQVLIQHPDVADVAVVGRPDAKWGEIPVAFVVK
                     KPDATLTAEEIIAHCKKNLASYKCVKEVQFVGAIPKNSLGKVLKKDLRSQFN"
     gene            2568..>3024
                     /locus_tag="PLG98_11815"
     CDS             2568..>3024
                     /locus_tag="PLG98_11815"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33996.1"
                     /translation="MSFADRNNPYSFNEFLAWRQGVDYYSDDPFIQKVVRHFTGDIWR
                     EVDAEARAVSRKASFRWRDIAERIARPEERPYMLHYDGHHNRIDRIVRPHDTEIMEKE
                     IFSEAFFADKTPPWVKLIKMYLIYQNGEACIGCPVTCTEGLVELLAKYAD"
BASE COUNT          730 a          786 c          824 g          684 t
ORIGIN      
        1 catatttcag atcgccgaaa tcccgaacag gcgatcggaa ggtaaagcct tttgctatcc
       61 tatgagcgac cagcgcggca atgtcatccg tcaggatgcc aagatgatgc acggcatttt
      121 tcccgtcctc ccgcggcgtc tggtcgtaaa aattgatgtg gccggccccg atgcggacga
      181 ggacattgcg cactcccgca acaatctcgt caaagacaat ttccgctccg aacatttcac
      241 gataaaaccc gatgccgcca tcaagattgg acgcgaagag gtggacatga tgcagatcgc
      301 agggcatgaa aattcctcca ttacttccca aaactcccgg caatgatgcg ggcaatgttg
      361 agcatgtgcc tgcgaaccgc tttggatgtg cggccgggat agttgcgaaa cgtaatgaga
      421 acaccgttta aagccgcaaa gaaggcgtgg gacaacagac gaacgggctc cggacaattc
      481 attttccgga agagtttgtc gaattgatca aaaatctggc gttccgcggc attgagtttt
      541 tcgatctttt ccggcgacag agatccgtcc agcatgaaat gcgtcatcat tctgaaatac
      601 tgatcattat ccatcagata ggtggtataa gcgtcgctga ttttgatgat atctggaatt
      661 tttgcctcct cgatcaatcc ctccagcgtg agcgtaattt ccttcgcccc ctgtaaaaaa
      721 gcttcgatga acaacgactg ctgatccgga aaataccggt agatggaggc atgagaaatt
      781 cccgcttcct ttgcaatatc gcgaatgttg actttgttga aaggtttgac ggcaaatacg
      841 cgctcggcgg cctgaatgat gaccgtctgg cgggcaagtt tttccccgtt tttcagatgg
      901 gcaaatgttt taggctgttt catggtcata tttcatatca taaaattttc tcaggatgaa
      961 aaaaaacttg acatcgtaac cagtggttag tacggtaacc aaaggttaca gattcgtcaa
     1021 aatcttttta tctaaggagg tcatgctcat ggaaatcaat gtaggcaaga tgctggcaaa
     1081 ccgcgcttac ctcagtcccg gcctggaggc tttctccggg atcgattatc gattcaactt
     1141 ccgcgaagcc aacgaacggg ccaaccgttt cggcgcttat ctgaagaaaa gcggcctggc
     1201 ctcaggcgat cggattgctg tcttatgcaa aaacaacgaa cacgccacga cggctctgtt
     1261 cggcgcggcc aagatcggga tcatcacgct catgctgaac tggcggttgc aggttccgga
     1321 actggactat attctcaaag acagcggcgc gtcgctgttg ctttacgatt cggaattcgc
     1381 gcccgtcgcc gaagcgctca aagggcagac ggccgtgaat cattttctga acgtcgagcc
     1441 gggaaacagg aataatgatt ttgaaaaggc cctggccggc gtatctgcgg ctgaaccgcc
     1501 ctacgcgggc ttcggagccg atccggcggt tctcatgtac acgtccggaa cgaccggcag
     1561 gcccaagggc gtcatgttga cgcatgacaa tctttgctgg gcgtcggtag gtctcgcgca
     1621 taccattgac tgggcgcaca aataccgttg gctttccgtc gcaccgctgt ttcacatcgg
     1681 cgggctgtcg ccgatctttg gcaacgtcca cgccggttgc acaacctttt ttgtgcccgc
     1741 gttcgacccg gtgaaaatct gggatgtgat tgtcgatgaa aaaataaact tcatgatgac
     1801 cgttccggcg atgctgcagt tcatgctgtt ggcgccggac atcgccaaaa aagacctgtc
     1861 gtcgatcagg cacatagtct gcggcggctc cgccgtgccg cagagtctga tcgaaaccta
     1921 cgacaagatg ggcatccagg tggagcaggt ttacggcatc acggaatatt caggcgccgt
     1981 gacgttctgg attcccgata tgggcatgga aaaatccaac tcggccggca agacggtctt
     2041 tcacggccag gttaaaatcc tgcgcccgga gagcgacgag gagatggcgg caggcgaagt
     2101 tggtgaaatc tgctgcttcg gcccgcaggt gttcaagggg tactggaata atccggacgc
     2161 gacaaaagcg gctgtcggcc gcggctacta ccgttccggc gacatgggca aaatcgacgc
     2221 cgatggtttt ctttacgtgg tggaccgcct gaaggacatg attatcagcg gcagtgaaaa
     2281 catttatccc gccgaactcg agcaggtttt gattcagcac ccggatgtgg ccgacgtggc
     2341 cgtggtgggc agaccggatg ccaagtgggg agagatcccc gtggcgttcg tggtgaagaa
     2401 gccggacgcg acgctcacgg cggaagagat catcgcccac tgcaagaaga acctggcatc
     2461 ctataaatgc gtcaaagaag ttcagtttgt cggggcgatc cccaaaaatt ctctggggaa
     2521 agtcctgaaa aaagatttac gttcacagtt caattgagga ggtcattatg tcgttcgcgg
     2581 atagaaacaa cccttattcc ttcaacgaat tcctggcctg gcgccagggt gtcgattact
     2641 attccgatga tccgttcatt caaaaagttg tgcgccattt taccggcgat atctggcgcg
     2701 aggtggatgc cgaagcgcgc gccgtctccc ggaaagcatc gtttcgctgg cgggacatag
     2761 ccgagcggat cgcgcgtccg gaagagcgcc cctacatgct ccattatgac ggccatcaca
     2821 accgcatcga ccggatcgtg cggccccacg acacggaaat catggaaaag gaaatttttt
     2881 ccgaagcgtt ctttgcggat aaaacacctc cctgggtcaa gctgattaaa atgtatctga
     2941 tttaccagaa cggcgaagcc tgcatcggct gccccgtcac ctgcacggaa ggcctggtcg
     3001 aactgctcgc caaatacgcc gatt
//
LOCUS       DAOSME010000164         2989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_35896_length_2989_cov_21.332311, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000164 DAOSME010000000
VERSION     DAOSME010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35896_length_2989_cov_21.332311"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PLG98_11820"
     CDS             <1..815
                     /locus_tag="PLG98_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE33997.1"
                     /translation="TGGLYYLFTFELMSQWVTYSFIHLLETMKGLFVYFLPLFALIVY
                     AIVKKEFRYGALNVFWIYILAGIPIFMFFLLNTGAETYYWYAVIPALTLAGVDVLHKE
                     CQKEKSWFAVAMLVLLFSLMTNKMIISETIKDGMVFPSEKLAVQWQPVVAEFDRNADR
                     ILNDDATAMLAIRAGKEPYRVGLGYLNIKNTLEKRFGYKFKDINAETANREYALIINP
                     ENESYVKQYYRLDRVHPVPVQLGKSYVLKMYVPGCCREKTASFNPAGLKNAK"
     gene            939..1739
                     /locus_tag="PLG98_11825"
     CDS             939..1739
                     /locus_tag="PLG98_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOE33998.1"
                     /translation="MSMLKKVFLRISGNRAVQGAIENKVQSLQELLGIGSGDGVLFSG
                     EQAVFDILRQKFNPPYCIFDVGANQGQFLRLLLEHIAVDDFTVHCFEPGRETFKILVA
                     SLSDDRRIQLNNTGLGKEQGEAILHFDSAGSGLASLTKRNLDHFGIDFNQSENVKIDT
                     IDHYCSENFIKHIHLLKIDVEGHELDVLAGAGRMFDEKSIDMVTFEFGGCNIDTRTFF
                     RDFWHFFRKINMKIMRITPSGYFYPIESYREIYEQFRTTNFIAVSGNS"
     gene            1753..>2989
                     /locus_tag="PLG98_11830"
     CDS             1753..>2989
                     /locus_tag="PLG98_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOE33999.1"
                     /translation="MLFNSYLFILVFLPVTAAVFFFLGRSGRSTWALGWLIGASLLFY
                     GWWNPWFVTVLIVSILFNYFWGIAVARGSKSLLAVGIAANLGLLGYFKYTNFFVENLS
                     AATGLSIELSPVVLPLAISFYTFQQISYLVDAYHGKISRHDFLNYCLFVTFFPRLIQG
                     PIVYYNELAPQFAQNEGFRLNPRMVAAGITIFFFGLFKKVIFADGLAAYATPVFEAAR
                     HGYVLSLMEAWEGALAYTLQIYFDFSGYSDMAIGLAFLFGIRVPLNFNSPYQSLNVID
                     FWRRWHMSLSRFLRDYLYIPLGGNRKGETMRFVNLMITMLLGGLWHGAAWTFVLWGGL
                     HGLFLIINHGWVKIKKRLGYEGKGGRAGRFFSGALTFLAVTVAWVFFRADSAATAFRI
                     IGSMAGQNGFALPEKYLDKF"
BASE COUNT          711 a          687 c          728 g          863 t
ORIGIN      
        1 tgaccggagg actgtactat ctctttacat tcgaactgat gtctcaatgg gtgacatatt
       61 cgtttattca ccttcttgaa acgatgaaag gcttgttcgt ttatttcctg cccctcttcg
      121 ccctgatcgt ctatgcgatc gtcaaaaagg aatttcgtta tggcgccctg aacgtttttt
      181 ggatttatat tcttgccgga atcccgatct ttatgttttt cctgctgaat acaggggcgg
      241 aaacctacta ctggtatgcg gttattcccg cgctcaccct ggcgggcgtc gacgttctgc
      301 acaaggagtg ccaaaaagaa aaatcatggt ttgccgtggc gatgctcgtt cttctttttt
      361 ctctcatgac gaataaaatg atcatttcgg aaaccatcaa agacggaatg gttttcccgt
      421 ctgagaaact ggccgtgcaa tggcagccgg ttgtggctga gtttgatcgc aacgcagatc
      481 gaatcctcaa cgacgatgca acggccatgt tggcgattcg tgcaggcaaa gaaccgtatc
      541 gggttggcct tggctactta aatatcaaaa atactttgga gaaaagattt ggttacaaat
      601 tcaaagacat taacgcggaa accgcaaata gagagtatgc cctgatcatt aacccggaaa
      661 atgaatctta cgtgaaacag tattatcgtc tggaccgagt ccaccctgtt ccggtccaac
      721 ttggaaaatc ctatgtcttg aaaatgtatg taccggggtg ttgtcgggaa aaaacagcct
      781 ctttcaatcc ggccggcctg aagaacgcaa aataatcttg tcttcgacaa caaaaggata
      841 tagtcagagg gcgaaatttc taatgaatca tttcactttc cggacgggaa ggcgatggcg
      901 gatcaggaag tatttcatga cggaggagaa gaaatcaaat gtcgatgctg aaaaaagtat
      961 ttctccggat ctcagggaac cgcgccgtcc aaggtgcaat cgagaacaag gttcagtcgc
     1021 tgcaggaact gttggggatt ggctccggcg acggcgtgtt gtttagcgga gagcaggccg
     1081 tttttgatat tctgcgacaa aaatttaatc ctccgtactg catttttgat gtcggtgcca
     1141 accaggggca attcctacgc ttgttgcttg agcatattgc cgttgatgat tttaccgttc
     1201 actgttttga acccggacgg gaaacattca aaatactggt cgcttcttta agcgatgatc
     1261 gacggattca actgaataat accggtttgg gcaaagagca aggagaagcc atactgcact
     1321 tcgacagcgc cggatccggt ctggcttcac tgacgaaaag aaacctggac cattttggca
     1381 tcgatttcaa tcaatcagag aatgtaaaaa tcgacactat tgatcattac tgttcagaaa
     1441 atttcataaa acacatccat ttactcaaga ttgatgtaga agggcatgaa cttgatgtcc
     1501 tcgccggggc aggaagaatg tttgatgaaa agtccatcga tatggttact tttgaatttg
     1561 gcggatgcaa tatcgacacc cgaacatttt tccgggactt ttggcatttc tttcgcaaga
     1621 tcaatatgaa aatcatgaga attacgcctt cgggatattt ttatcccatt gaatcctata
     1681 gagaaattta tgaacaattt cggacaacaa attttatagc agtgtcgggg aattcataaa
     1741 ggttgccttc ccatgctctt taattcctat cttttcattc tggtgttcct gccggtcact
     1801 gcagcggtat ttttctttct tggccgcagt gggcgctcga cttgggcgtt ggggtggttg
     1861 atcggcgctt cccttctttt ttacggctgg tggaatccct ggtttgtgac ggtgttgatc
     1921 gtctccattc tgtttaacta cttctggggg atcgccgtcg cccgcggttc aaaaagcctt
     1981 ctggcggttg ggattgcggc aaacctcggg ctgctcggct atttcaaata caccaatttt
     2041 tttgtagaga acctgtccgc ggccacaggt ttgtccatcg aactctcccc ggtcgtgctg
     2101 cctttggcga tttcgtttta tacgtttcaa caaatatcct atctcgtgga cgcctaccat
     2161 ggaaagatca gcaggcatga ttttttaaac tactgcctgt ttgtgacttt tttccccagg
     2221 ctcatccagg gacccattgt ttactataat gaacttgcgc cccagttcgc gcaaaacgaa
     2281 ggattccgat tgaacccgcg tatggtggcg gcgggcatca ccattttttt ctttggcttg
     2341 tttaaaaaag tgatctttgc cgacgggctg gcggcatacg cgacgcccgt atttgaggcg
     2401 gcgcgacatg gctacgtgct ttcattgatg gaagcctggg aaggagccct agcctataca
     2461 ttgcaaatct attttgactt ctccggctac tccgatatgg ccattggcct ggcctttctg
     2521 ttcggcatcc gcgttcccct gaattttaat tctccctatc aatccttgaa cgtcatcgat
     2581 ttctggcgaa gatggcatat gtcgctttcc cgttttttgc gggactatct ttatattccc
     2641 ttgggcggca accgcaaggg agaaaccatg cgctttgtca atttgatgat caccatgctg
     2701 ctgggagggc tttggcacgg cgcagcctgg acctttgtcc tgtggggcgg tttgcacggt
     2761 ttatttctaa tcatcaatca cggatgggta aaaatcaaga agcggttagg ttacgaagga
     2821 aaaggcggaa gggccggccg ttttttctcc ggagcgctta cgtttcttgc cgttacggtt
     2881 gcctgggttt ttttcagggc cgacagcgcc gccaccgcat ttcgcatcat cggttccatg
     2941 gcgggccaaa acggctttgc cctgccggaa aaatacctcg ataagtttg
//
LOCUS       DAOSME010000165         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_35913_length_2988_cov_26.986362, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000165 DAOSME010000000
VERSION     DAOSME010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35913_length_2988_cov_26.986362"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLG98_11835"
     CDS             <1..618
                     /locus_tag="PLG98_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34000.1"
                     /translation="SYAVLDHRIIGFCAGSLFVHDAIFIYSNDETMVLREFHHRNIAR
                     NMVFCNMRWILQSRHDEGIKHYAFMSISGNPRIVNGYYNHSFLIKILFDCSFKASDKL
                     IRALDAYRHQYKISLVHEDYPFCLKNMFPGSNTFNPSDQRFQFLKRVKRNMPADFDHM
                     ERGDAFAFLVKLPAWFTRTFVFGLMVLAFGRRFFSRRGIGFFAGN"
     gene            complement(671..1690)
                     /locus_tag="PLG98_11840"
     CDS             complement(671..1690)
                     /locus_tag="PLG98_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE34001.1"
                     /translation="MTSPEKIAQPVLVTGATGFIGSRVVQKLLQNETAVKILALPGEA
                     VPAAWAEKIEIVRGSISDPRAVEKAANGAKTIIHLVAIVTDWGDEKEYWEFTVEGSRL
                     IFDEAVKQNARIVLASSIAVYGDNIYKQPCPEDTPYGNTLGPYSRTKQAQEKLAWNYY
                     RNKGMKLTVVRPANVYGPRSGPWLHDVVNVLRSGSPGLISGGQMNAGLAYVDNVADIL
                     ILAGTFEKALGNVYNACDGLDVTWKRYFTDIANIIGVGNPKSLPRPLAVLGAYLLEGI
                     YKLLGIKKRPLITREALNLVGSDNRIPIDKIRRELGYEPVISYAEGLKHIREYLHQDP
                     PGKKT"
     gene            complement(1724..2662)
                     /locus_tag="PLG98_11845"
     CDS             complement(1724..2662)
                     /locus_tag="PLG98_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOE34002.1"
                     /translation="MKENEIKSPLILEIKGNSLDDGPGIRSVVFYKGCPLSCVWCHNP
                     ESKKAVKEISFDAKTCIDCGACREVCPKNALGRDNPFYIDRSKCDLCFLCLDVCPSGA
                     LDSVGKAMSINDIMKKILPDKPFFDTSGGGVTLSGGEPTLFMDFTSGLLKSLKQNNIH
                     TLLETCGFFDIHKFMDRLYPYLDAIYFDIKIIDREAHKKYCGVSNETILDNFRLLSRQ
                     AADDGKMFLARTPLIPDMTDTESNIQGIASFLKSLNITEAALLAYNPLWHEKSDKVGV
                     NDPYKHSKVMTSFRDNAVMERCKTIFGNAGITETKR"
BASE COUNT          685 a          725 c          744 g          834 t
ORIGIN      
        1 agttatgccg tgctggatca ccggattatt ggtttttgcg cgggcagtct tttcgtccat
       61 gacgccatct ttatctacag caatgatgag accatggtgc tcagagagtt tcatcaccgt
      121 aatattgcca gaaacatggt cttctgcaat atgcgctgga tattgcaaag caggcacgat
      181 gaaggcatca aacactacgc gttcatgtcg atttcaggca atcccagaat cgtgaatgga
      241 tattacaacc atagttttct aataaaaatc ctattcgatt gttcctttaa ggcgtcggat
      301 aaattaatcc gtgccctgga cgcctaccgt catcaataca agatatcgct ggtgcatgag
      361 gattatccct tttgtctcaa aaatatgttt ccgggatcga acaccttcaa tccgtcggat
      421 caaagatttc aatttctgaa aagggtcaaa aggaatatgc cggctgactt tgatcacatg
      481 gagcggggag atgcctttgc gtttctggtg aagttgccgg catggtttac acgtacgttt
      541 gtcttcggcc tgatggttct tgccttcggc aggcggtttt tctcccgtcg cggcataggc
      601 ttttttgccg ggaattaact ctgaataaac gcgaacgact tcaaaacggt catgactcca
      661 aaaaacgcgt ttaagttttt ttacccggcg ggtcttgatg aagatactcc cgaatatgct
      721 ttaatccttc ggcataggaa atgacgggtt catacccgag ctcgcgtcga attttgtcga
      781 tcggtatccg gttgtctgag cccaccagat tcaaggcttc gcgcgtaatg agaggacgtt
      841 ttttaatgcc cagcaattta taaattcctt ccagtaaata agctccaagg actgccagcg
      901 gccgggggag ggattttgga ttgcccacgc cgatgatgtt ggcgatgtcc gtaaaatatc
      961 gtttccacgt tacgtccagc ccgtcgcagg cgttgtaaac gttgcccagt gccttttcga
     1021 aagttcccgc aaggatgagg atatccgcca cattatcgac ataggccagt cccgcgttca
     1081 tctgtccgcc ggagatcagg ccgggcgatc cgctgcgaag gacattcacc acgtcgtgaa
     1141 gccaggggcc ggagcgcggc ccgtagacgt tggccggacg aaccacggta agtttcatgc
     1201 ccttgttccg gtaataattc caggcgagct tttcctgcgc ctgttttgtc ctgctgtagg
     1261 gtcccagtgt gttgccgtaa ggcgtatctt ccgggcaggg ctgcttgtaa atgttgtcgc
     1321 cataaacggc tatgcttgag gcgagaacga tccgggcgtt ttgttttacc gcctcatcaa
     1381 aaatcaaacg gctgccttcg acggtaaatt cccagtattc tttttcatcg ccccagtcgg
     1441 ttacgatagc caccagatga ataatggttt tagctccgtt ggcggccttt tccacagccc
     1501 tgggatcgga gatgcttccc cggacaattt caattttttc cgcccacgca gccggcacgg
     1561 cttcaccggg cagcgccagt attttgacgg cggtttcgtt ttgtaatagt ttctgaacga
     1621 ccctgctgcc gatgaatccc gtggcgccgg tgaccaaaac cggttgtgct attttctctg
     1681 gcgacgtcat tgtgtgattt cctcccgttt atgatacgcg gtattaacgt ttggtttctg
     1741 ttatgcccgc atttccgaag atggttttac atctttccat gacggcgttg tcgcgaaaag
     1801 acgtcatgac cttgctgtgt ttgtaaggat cattcacgcc gactttgtcg cttttttcat
     1861 gccataacgg attataagcg agaagagcgg cttcggtgat gttaagactt ttcagaaacg
     1921 acgcaatgcc ctgaatgttg ctttcagtgt ctgtcatatc gggaatcagc ggggtgcgcg
     1981 ccagaaacat ttttccgtcg tccgctgcct gccgggaaag gagtctgaaa ttatccagaa
     2041 tcgtttcgtt ggacacgccg cagtattttt tatgcgcctc acggtcgatg attttgatat
     2101 cgaaataaat agcgtccaga tacggataaa gcctgtccat aaatttatgg atgtcaaaga
     2161 aaccgcaggt ttccaaaagg gtgtgaatgt tgttttgttt gagcgatttc aaaaggccgg
     2221 aggtaaagtc cataaaaagc gtcggctcgc ctccggaaag ggttaccccg cctcccgatg
     2281 tatcgaagaa gggtttatcc ggcaggattt ttttcataat atcgttgatg gacattgctt
     2341 tcccgacgct gtccagcgca cccgaggggc agacatcaag gcacagaaaa cacagatcgc
     2401 atttgctgcg gtcgatgtaa aaaggattat cgcggcccag cgcgtttttc ggacaaacct
     2461 cgcggcaggc tccgcagtcg atacaggttt ttgcgtcaaa ggatatttcc ttgacggctt
     2521 ttttactttc cgggttgtga caccacacgc aggaaagagg gcagcctttg taaaacacga
     2581 cggaacggat acccggtccg tcatccaggc tgttgccttt gatttcgaga attaaagggg
     2641 atttgatttc attttctttc ataatcaatt gtttttccca ctagtgactg tttcgtaaaa
     2701 aaaatcacaa ctcatggacc cgtcattccg gcggaggccg gaatccagtg ttatctgaac
     2761 tggcccccgg ccgacgccgg ggtgactaca ttttgcactt ttgccgagtt catccaatat
     2821 tgatctatat gccgaattcc gcgcgttcga tcaattcgat ctgcatttct ctgttcaacg
     2881 ttacaaaata ggcgttgtag ccggagatgc ggacaagcag attctgataa ttctccggat
     2941 gttccatggc gtcgcgaagc gtttttgacg acacgacgtt caactgca
//
LOCUS       DAOSME010000166         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_35952_length_2985_cov_25.250171, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000166 DAOSME010000000
VERSION     DAOSME010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35952_length_2985_cov_25.250171"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2597)
                     /gene="alaS"
                     /locus_tag="PLG98_11850"
     CDS             complement(<1..2597)
                     /gene="alaS"
                     /locus_tag="PLG98_11850"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOE34003.1"
                     /translation="MAKTGNEIRESFLKYFESKGHTVVASSSLIPKDDPTLLFTNAGM
                     VQFKNAFLGLENRGYTRAATCQKCARAGGKHNDLENVGVTARHHTFFEMLGNFSFGDY
                     FKEEAIAWAWEYLIDVVKLPQEKLWITIYEDDDEAFEIWNKKMNVPADRIVRMGEKSN
                     FWMMGETGPCGPCSEILFDQGEGTGCGRPECDVHCDCDRHLEIWNLVFTQFDRDENGK
                     LTPLAKPSIDTGMGLERLTAVIQGKKSNYDTDLFTPIIRFVEKISGKTYGQNTEDDIS
                     IRVIADHSRAVTFLIGDGITPSNEGRGYVLRRILRRAARHGKMLGINKPFLNESARVV
                     IDMMKGVYPDLVEKASFITKMILNEEQRFMETLDAGLRILNEETEALKKAGKSVLPGA
                     LVFKLYDTYGFPTDLTADIVKRDGFTLDNEGFEKEMEAQRERARGAWRGSGEEAVAEC
                     YLKVSSSGVISEFCGYEKIKKDNAEIRAIFIDGKPVETAGEGQKAEIVLDNTPFYGEA
                     GGQTGDTGYLKGAGFNFTVEDTKKPVENFIVHKGVVSKGAINVGAKAKLVVDEERRKA
                     IAANHSGTHILQAALKAVLGDHIKQSGSQVTAEGLRFDFTHFSKIGDEEMKQIEEIAN
                     EMIRKNVDVKTEVCALEDALKTGATAVFDEKYGSTVRIVKMGKMSMELCGGTHVKRTG
                     DIGFLKVVHESAIAAGVRRIEAVTGREAFKHFRRTEEELKRTAGLFKASQMEVYDRAE
                     KLQKHTKELEKEIETLKGKLAAKDSGDLLGQAKEINGVNVLATEVSITDAKTLRDFGD
                     KLRDKLGSGIILLGSKAGDKVMLLCMVTKDLTGKYHAGNIIKELAPMVGGSGGGRPDM
                     AQAGGSQPE"
     gene            complement(2578..>2985)
                     /locus_tag="PLG98_11855"
     CDS             complement(2578..>2985)
                     /locus_tag="PLG98_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOE34004.1"
                     /translation="LREKGFPAVVIKETLEKLHDLKYLNDASFANQWARNLAVNKLWG
                     NKKIIASLREKGLTAELMELSIEKAREELPEEAALEILIKKKAAKNKSAALDFKEKQK
                     IFQALMGRGFPPGLILNKLGRIKKEDIDGKDWE"
BASE COUNT          594 a          831 c          710 g          850 t
ORIGIN      
        1 ttttccggct gggaaccgcc ggcctgagcc atgtcgggac ggccaccgcc gctgccgccg
       61 accatcggtg ccagttcctt gatgatgttt ccggcatgat atttcccggt taaatccttg
      121 gtcaccatgc atagcagcat gactttatcg ccggccttgc tgcccagaag gatgataccg
      181 gagcccagct tgtcgcgcag cttgtcgccg aagtcgcgca atgttttggc atcggtgatg
      241 ctgacttccg tcgccagcac gttcactccg ttgatttctt ttgcctgacc gagcagatcg
      301 ccggaatcct tggcggcgag cttccctttg agcgtttcga tttctttttc cagttctttg
      361 gtgtgctttt gcagtttttc ggcgcggtcg tagacttcca tctgactcgc tttgaacaat
      421 cctgccgtac gttttaattc ttcttcggtt cgccggaagt gcttgaaagc ttctctgccg
      481 gtgacggctt caattcggcg tacgcccgcg gcaatggctg attcatgaac aactttcaaa
      541 aagccgatat ctccggtgcg tttcacatgt gtaccaccgc agagctccat gctcattttg
      601 cccattttca caatgcgcac agtggagccg tatttttcat cgaacacggc cgtcgcgccg
      661 gttttgagcg catcttcaag cgcgcagact tccgttttga catccacatt tttgcggatc
      721 atctcgttgg cgatttcttc aatctgcttc atttcctcat cgccgatttt ggaaaaatgg
      781 gtgaagtcga accgcaatcc ttccgccgta acctgcgaac cggattgctt gatgtggtct
      841 cccaataccg ctttgagtgc cgcctgcagg atgtgcgtgc cggaatggtt ggcggcaatg
      901 gctttgcgtc tttcctcgtc aacaaccagt tttgcttttg cgccgacatt gattgcaccc
      961 ttgctgacca cccctttgtg cacgatgaaa ttctcgacgg gctttttcgt atcttcgacg
     1021 gtgaaattaa atccggctcc cttgagataa ccggtatctc cggtctgacc tccggcttct
     1081 ccgtaaaaag gcgtattgtc cagaacgatt tccgccttct gtccttcacc cgccgtttcc
     1141 accggtttgc cgtcaataaa tatcgccctg atttccgcat tgtctttttt gattttttca
     1201 taaccgcaga attcagagat aacaccggaa ctggaaactt tgagatagca ttcagcaacc
     1261 gcttcttcgc cgctgcccct ccaggcgccg cgggctcgtt cgcgctgcgc ttccatttct
     1321 ttttcaaagc cttcattgtc cagcgtaaag ccgtcgcgct tgacgatatc cgccgtcaaa
     1381 tccgtgggga agccgtaagt gtcgtagagt ttaaaaacca aagcacccgg gaggaccgat
     1441 ttaccggctt tcttcagcgc ttccgtttct tcattcagga tgcgcagacc cgcgtcgagc
     1501 gtttccataa aacgctgttc ttcatttaaa atcattttgg tgatgaagga ggccttctcc
     1561 accagatcgg gataaacgcc cttcatcatg tcaatgacga cccgggcgct ttcgtttaaa
     1621 aatggtttgt tgatgccgag cattttgccg tggcgtgcgg cgcgccgcag aatgcgtctg
     1681 agcacgtagc cgcgtccttc gttgctgggc gtgatgccgt cgccgatcag gaaagtcacc
     1741 gcgcggctgt gatcggcaat gacacggatg gaaatgtcgt cttcagtatt ctggccgtaa
     1801 gtcttgcccg atattttttc cacaaagcga atgatcggcg tgaaaagatc cgtgtcgtaa
     1861 ttgcttttct tgccttgaat gacggcggtc agacgttcca gtcccatacc ggtgtcgatg
     1921 ctgggtttgg ccagcggcgt taactttccg ttttcatcgc ggtcgaattg cgtaaagacg
     1981 agattccata tttccagatg acggtcgcaa tcgcagtgga catcgcattc cggacggccg
     2041 cagccggtgc cttcgccctg atcaaacagg atttcggagc aggggccgca ggggccggtt
     2101 tcgcccatca tccagaagtt gcttttctcg cccatgcgga cgatgcgatc cgcgggaaca
     2161 ttcatctttt tgttccagat ttcaaacgcc tcatcgtcat cttcataaat cgtgatccac
     2221 aatttttcct ggggaagttt gacgacatca atcagatatt cccacgccca ggcgatggct
     2281 tcctccttga aataatcgcc gaaggaaaaa ttgccgagca tttcgaaaaa ggtatgatga
     2341 cgggcggtaa cgccgacatt ttccaggtcg ttgtgctttc cgccggcgcg cgcgcatttc
     2401 tggcaggttg ccgcccgggt gtagccccgg ttttccagtc ccagaaacgc gtttttgaac
     2461 tgtaccatgc ccgcgttggt gaaaagcaga gtaggatcgt ccttgggaat cagcgacgaa
     2521 cttgccacca cggtgtgccc cttgctttcg aaatatttta aaaaactttc cctgatttca
     2581 ttcccagtct ttgccatcaa tatcttcctt ttttatcctt cccaatttat ttaaaatcag
     2641 ccccggcgga aaaccgcgtc ccatcagagc ctgaaaaatt ttctgttttt ccttaaaatc
     2701 caaagcagcc gacttatttt ttgccgcttt ctttttgatt aaaatttcaa gcgccgcttc
     2761 ttccggcaac tcctcgcgcg ccttttcgat ggacaattcc attaattcag ccgtcagacc
     2821 tttttcccgc aagctcgcga tgatcttctt gtttccccag agtttgttga cggcaagatt
     2881 gcgcgcccac tggttggcaa aagacgcgtc attgagatac ttcaaatcgt ggagtttttc
     2941 cagtgtttct ttaataacaa ctgcgggaaa acctttttcc cgcag
//
LOCUS       DAOSME010000167         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_36824_length_2930_cov_17.871652, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000167 DAOSME010000000
VERSION     DAOSME010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36824_length_2930_cov_17.871652"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLG98_11860"
     CDS             <1..971
                     /locus_tag="PLG98_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOE34005.1"
                     /translation="FDQDDEINLLELWQVIWKRRKFIAYFVGVVVVLTAIVSLFMTNI
                     YESKAVITPVDSKSSAGGGMAAALAAMGGGLGGLGGLIGLPESASSTEIMLLLNSNIL
                     REKIIKDYNLMPVLFSDQWDAEKKAWKKGGGFSLNPLTWISKLIAAVKPADKKGVKKE
                     PGVPDMWDGLRALKDLVRIQSNVKEKSITISAQFDDPEIAARLAGYFVAALNDHMSKE
                     ARRVAAINKKYLEEQLVLNTDPLIDQKIYTMIAQQLETSMMAEVKENFSFKVIDPPFV
                     PDRKIKPKRAQMVMLSFVVALFLAVFIVFFLEYLEKMKKVDGPKTE"
     gene            1382..>2930
                     /locus_tag="PLG98_11865"
     CDS             1382..>2930
                     /locus_tag="PLG98_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOE34006.1"
                     /translation="MFNNLTPAQKQALMQQQSKQDNLTPAQRQALLKQQADQTGLTPG
                     QQQVEKIELTPEQLRVIQHVLSKTRGLLTPQAIEVLRQSPEFREFKWEDFAKSKDLIE
                     KSFIPQELPEPKPSFFEKSRMVGKYQDIDLQLLPFGYEFFKDAAVGVVTERKDVPVPA
                     TYIVGPGDEVKIMLWGRVNAQYNLTIDRNGSINIPSIGPLYVAGMTFEKMSTYLIQQA
                     TQIVGANIDVSMGALKTIPVFILGDVKRPGAYTIGSFSTITDALLMAGGPTEIGSMRN
                     VQLRRKDRIVTTFDLYSLLLRGDKSADAVLRAGDVVFVPVSGPLVGIAGNVKRPAIYE
                     LKENHNLHNALELAGGILPSAYTQQIQISRIQKNQREVVVDIDDRHMQKAEAFHLQDA
                     DLVKIFTIVDKDQNSVYLEGNVKKPGRYEFRKGMKISDLIKDNADLLDETYYDYALVK
                     RLILPQRSTQLVPFNLGKIILNHDKQENLELHPEDRVYIYSKWLFKEKPYVTIEGEIR
                     GLCSSSFE"
BASE COUNT          801 a          591 c          769 g          769 t
ORIGIN      
        1 attttgacca ggatgacgag atcaatctgc tggagctgtg gcaggtcatc tggaagcgca
       61 ggaagttcat cgcatatttt gtcggcgtcg tggtggtgct gacggcgatc gtatcgcttt
      121 ttatgacgaa catctatgaa tccaaggctg tcatcacgcc ggtggattca aaaagcagcg
      181 cgggtggcgg catggcggcg gcattggcgg caatgggggg cggattgggt ggcctgggtg
      241 gacttattgg tcttcctgaa tcggcatcgt caaccgaaat catgctgctt ttgaactcca
      301 atattctgcg tgaaaaaatt atcaaagatt ataatctgat gccggtttta ttcagcgatc
      361 aatgggatgc agagaaaaaa gcttggaaaa aagggggagg attcagcctt aatcctctga
      421 cgtggatttc caaactgatc gcagcggtaa aacctgcgga caaaaaaggt gttaaaaaag
      481 agccgggtgt tcccgatatg tgggatggat tgcgggcgct gaaggatctg gttcggatcc
      541 agagcaatgt caaggaaaaa tcaattacga tctcggcgca gtttgatgat ccggaaattg
      601 ccgcccgtct ggccggatat tttgtcgcgg cgctcaacga tcatatgagc aaagaggcga
      661 gaagggttgc ggctatcaac aagaagtatc tggaagaaca gttggtgctg aataccgatc
      721 ctctgatcga tcagaagatt tacacgatga tcgcgcagca gttggagacg tccatgatgg
      781 cggaggtgaa ggaaaatttc tctttcaagg tgatcgatcc gccgttcgtg ccggacagga
      841 agattaagcc caaaagggcg cagatggtaa tgctgagttt tgtggttgcg ctgtttttgg
      901 ccgtgtttat cgtgttcttt ctggaatatc tggagaaaat gaaaaaggtt gatggtccga
      961 agactgaatg aaaaacaagg ggttgcaacc ccttgttcta agggttggtc gactgaagac
     1021 tgaaggtaaa gagagagctt tgcataatca caattttgtc attccggtga aaaccggaat
     1081 ccagtattat tggtgatttc atagataccg gctcgcgtgc cctttagggt attcgccaga
     1141 ccttatgaca agtttttggg tttttttata agactatcga gacatggtct ttatgtagta
     1201 cgggccttca ggctcgttac tcattgctca aataataggg cgctgtccct aatttttcgg
     1261 aggggtgaca ttgtataaaa aaatatcagc aatcattgca ttaatgattt tatttgtttt
     1321 gcatagcgct gtttatgctc aggcacctgt ttcacctgcc aatcaacagg cggctcagga
     1381 aatgttcaac aatttaacgc ccgcgcaaaa gcaggctctc atgcaacagc agtctaagca
     1441 ggataattta acgccggcac aaaggcaggc tcttctgaaa cagcaggctg atcaaactgg
     1501 tttaacaccg ggacaacagc aagttgaaaa gattgaatta acaccggagc aactgcgggt
     1561 tattcaacac gtcctcagca agaccagagg tcttttaacg ccgcaggcaa tcgaagtgtt
     1621 aaggcaatcg ccggaatttc gagaattcaa gtgggaagat tttgctaaat cgaaagatct
     1681 gatagaaaaa agttttatcc ctcaagaact gcccgagccc aagccttctt tttttgaaaa
     1741 gtcgcgcatg gtaggaaaat atcaggacat tgatctgcaa ttgctgcctt tcggctatga
     1801 attctttaag gatgccgccg taggtgttgt aacggaacga aaagatgttc ctgttcctgc
     1861 gacgtatatt gtcggtcccg gcgacgaggt gaagataatg ctctgggggc gggttaatgc
     1921 ccagtataat cttactattg accgaaacgg cagtatcaat attccctcca ttggtccgct
     1981 ttatgttgcc ggaatgacct ttgaaaaaat gtccacctat ctgatccagc aggcgacgca
     2041 aattgtgggc gccaatattg atgtcagcat gggcgcttta aaaacgattc cagtgtttat
     2101 tctgggggac gtgaaaaggc cgggcgccta tacgatcggt tctttttcaa ccattacgga
     2161 cgctcttctg atggccggag ggcctacgga aataggctcc atgcgaaatg ttcagttaag
     2221 gcgaaaagat cgaatcgtta caacttttga tttatatagc ctccttctcc ggggcgataa
     2281 atcagcggat gcggttctgc gggcggggga tgtcgttttt gttccggtct ccggtccgct
     2341 ggtgggtatc gccggtaatg tgaaaaggcc ggccatctac gagcttaaag aaaatcacaa
     2401 tctgcacaac gccctggagc tggccggcgg cattctgccc agtgcctaca cgcagcagat
     2461 acaaatttcg agaattcaga aaaatcaaag agaggttgtt gttgatattg atgatcgcca
     2521 tatgcaaaag gcagaggcgt ttcatttgca ggatgccgac ctggtgaaaa tatttaccat
     2581 tgtcgataaa gaccaaaatt ccgtatatct tgaggggaat gttaaaaagc cgggtcgcta
     2641 tgaatttaga aagggaatga aaataagcga tctaattaag gataatgccg atcttcttga
     2701 tgaaacgtat tatgattacg ccttggtgaa gaggctaatc ttaccccagc gcagcaccca
     2761 gcttgtgccg tttaatttgg gcaaaattat tttaaaccat gataagcagg agaatctgga
     2821 acttcacccc gaggaccgtg tttacattta ttcgaagtgg ttattcaaag aaaaaccata
     2881 tgttacgatt gaaggtgaga taaggggtct ttgctcatcc tcatttgagg
//
LOCUS       DAOSME010000168         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_36915_length_2924_cov_13.066574, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000168 DAOSME010000000
VERSION     DAOSME010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36915_length_2924_cov_13.066574"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..989)
                     /gene="lipA"
                     /locus_tag="PLG98_11870"
     CDS             complement(114..989)
                     /gene="lipA"
                     /locus_tag="PLG98_11870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOE34007.1"
                     /translation="MTMISGQPKSQLLRKPPWLKVRPPYDDACRRIRSTLSGLQLHTV
                     CQEAACPNMAECFACGTATFLILGNICTRNCLYCNVAHGRPQPPDEQEISRLISAVKQ
                     MRLQYVVLTSVTRDDLPDGGAGAFADAIRRLRGEIPGCKIEVLIPDFGGDPNALGLVI
                     DAAPDVINHNLEAAEPMFDKLRPQGNYRLSLDLLSRVAAAGLVTKSGFMVGFGEQKED
                     VLRLMDDLAEVSCARLTIGQYQQPTRRHWPVARYYTPEEFAEFGKIAYAKGFEHVESG
                     PLVRSSYHAARAVSR"
     gene            complement(998..2227)
                     /locus_tag="PLG98_11875"
     CDS             complement(998..2227)
                     /locus_tag="PLG98_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE34008.1"
                     /translation="MYIKRTLETKITKYLKTPEIIAVTGARQVGKTTLLQQIHQGLDN
                     SLFITFEDVSYRQLFDTDIQAFIELYIQPYKYIFIDEFQYANNGGQQLKRIFDTVQGK
                     KIFISGSSVLDLTIHAVKHLAGRLFAFVLHPLSFEEFLAFKNQELHRYLSGKKENAKF
                     SSIMLEKLYAYLEEYIVYGGYPRVLLSPDHEEKKEVLRNILNVYLLRDVRDLMGLFDD
                     YKMISLMKALSLQTGNVISYQELSQITSLNFSSLKKNLNLLEKTYVIGLPRPFFTNKR
                     IELVKNPKIYFHDTGLRHAVIDDFRRMEMRQDKGALWENFIYAELMKEGNDIKYWRTK
                     SKAEVDFIVNEKIPVEVKSSPTKTAVGKSLHSYIEKYNPDNAYIFTKKTEESFMIGKT
                     AVHFLHHFSKIPFAEQH"
     gene            complement(2327..>2924)
                     /locus_tag="PLG98_11880"
     CDS             complement(2327..>2924)
                     /locus_tag="PLG98_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34009.1"
                     /translation="GVAEKLVDFLPVPRVVKNGEEYSLSKDFPYTIGRVRAFYGNFTV
                     IVKAYAYLRSLGEEGLRRATEMAVLNANYIKERLKSYFDLPYDRVCMHECVFSGNRQV
                     AESGVHTSDIAKRLIDFGYHPPTIYFPLIVPEAIMIEPTETESKETLDAFCDAMIAIA
                     KEAKENPQRIKDAPLSTPVGRLDEVLAARKPDVCWERS"
BASE COUNT          678 a          701 c          779 g          766 t
ORIGIN      
        1 gaagcaagag agacagaaga tcaaaggcat gcggtaggcc atcatacgat ctcctttaag
       61 tgccgccgag tatatggggc ggcacagggg ctgtcaagag aatgtttggg cgattaacgg
      121 gagacggcgc gggccgcgtg gtaggaactg cgcaccagcg gaccggattc cacgtgctcg
      181 aagcctttgg cgtaagcgat tttcccgaat tcagcgaatt cctcgggcgt gtaatatctg
      241 gcgaccggcc agtgccgccg cgtcggctgc tgatactgcc cgatggtaag ccgcgcgcag
      301 gacacctcgg cgagatcgtc catcaggcgc aggacatctt ctttttgctc cccgaaccca
      361 accataaaac cgcttttggt gacgaggccc gcggccgcta cgcgggaaag aagatccagg
      421 gagaggcggt aattcccctg cgggcgcaat ttgtcaaaca tcggttctgc cgcctccagg
      481 ttgtgattga tcacatccgg cgccgcatcg atgacgagac ccagcgcatt cgggtctccg
      541 ccgaaatcgg gaatcaatac ttcaattttg cagccgggaa tttctccgcg caggcggcga
      601 atggcgtcgg caaaagcccc cgcgccgccg tccggcaaat catcccgcgt tacggatgtc
      661 agcaccacgt attgcagccg catttgcttt acggcgctga tcaggcggct gatttcctgc
      721 tcgtccggcg gctgcggccg gccgtgggca acattgcagt acaggcagtt gcgcgtgcaa
      781 atatttccga gaatcagaaa ggtggccgtg ccgcaggcaa aacattccgc catgttcgga
      841 cacgcagcct cctggcagac ggtgtgcagt tgcaggccgg agagcgtcga gcggattcgg
      901 cggcaggcgt cgtcataagg cggccgcacc ttgagccagg gcggcttgcg caagagttga
      961 gatttcggct gtccgctaat cattgtcatt tttccgctta atgttgctcg gcaaacggta
     1021 tttttgaaaa gtgatgaaga aaatgaacgg cggtcttgcc aatcatgaac gattcttcag
     1081 tttttttggt gaaaatatag gcgttgtccg ggttgtactt ttctatatag ctgtgcaggg
     1141 acttcccgac ggccgttttt gttggcgagg attttacttc caccggaatt ttttcgttta
     1201 caataaaatc cacttccgct ttggattttg ttcgccaata tttgatatca ttgccttctt
     1261 tcatcaattc cgcatagatg aaattttccc agagagcgcc tttatcctgt ctcatttcca
     1321 tccggcgaaa atcatcaatg acggcatgac gcagcccggt atcatggaaa tatatttttg
     1381 gattttttac cagttcaatc cttttgttgg tgaaaaaggg cctcggtaat ccgataacat
     1441 aagttttttc cagaagattg agattttttt tcaaactgct gaaattgaga gatgtaatct
     1501 gggaaagctc ctgataggag atcacattgc cggtttgcag gctaagcgct ttcatcaggc
     1561 tgatcatctt gtagtcgtca aacaatccca tcagatctct gacatcgcgc aaaagataca
     1621 cgttgagaat gttgcgcaga acttcttttt tttcttcgtg atccggagaa agcaaaacgc
     1681 gcgggtatcc gccatacacg atgtattctt caagataggc atagagcttt tccagcatga
     1741 ttgagctgaa tttggcattt tctttcttgc cgcttaggta acggtggagc tcctgatttt
     1801 taaaagcaag aaattcttca aagctgaggg gatgaagtac aaacgcgaac aggcgccccg
     1861 caagatgttt tacggcatga atggtcagat cgaggacaga cgaaccggaa ataaaaattt
     1921 ttttcccctg gacggtatcg aatatccgct tcagttgctg tccgccgttg tttgcgtatt
     1981 gaaattcatc aataaatata tatttgtaag gttggatata aagttcgata aatgcctgaa
     2041 tatccgtatc gaacagctgc cggtatgaga catcttcaaa ggtaataaaa agagaattgt
     2101 ccagaccttg atgaatttgc tgtaagagtg tcgtttttcc tacctgacgg gcgccagtaa
     2161 cggcaatgat ttcaggtgtc ttcaggtatt tggttatttt tgtttcaagg gtccttttga
     2221 tatacattat tttacctgtt attttgtttt ctgaaataat agcagggtta tcattatgtt
     2281 tcaagaaata atgacatgct tgttgcaggc atggaaaatg acgccgctac gacctttccc
     2341 agcacacgtc cggttttcgg gcggccagaa cttcgtcgag gcggccaacc ggcgtggaaa
     2401 gcggcgcgtc cttgattcgc tgcggatttt ctttggcctc ttttgcaatg gcgatcatgg
     2461 cgtcgcagaa ggcatcgagc gtttctttgc tttcggtttc ggtaggttcg atcatgatgg
     2521 cttccggcac gatgagcggg aaatagatcg tgggcgggtg gtagccgaaa tcgatcagcc
     2581 gcttggcgat gtcgctggtg tgcacgccgg attcggccac ttggcggttt ccggaaaaaa
     2641 cgcattcgtg catgcaaacc cggtcgtagg gcagatcaaa ataagatttt aatcgttcct
     2701 tgatatagtt ggcgttcagc acggccattt ccgtcgcgcg ccgaaggcct tcctcgccca
     2761 gcgagcgaag gtaggcatag gctttgacga tgaccgtaaa gtttccgtaa aaggcgcgga
     2821 cgcggccgat ggtataggga aaatctttcg acagactgta ctcctcgccg tttttcacca
     2881 cgcggggcac cggcaggaaa tcgaccagct tttccgccac gccc
//
LOCUS       DAOSME010000169         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_37169_length_2910_cov_15.983187, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000169 DAOSME010000000
VERSION     DAOSME010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37169_length_2910_cov_15.983187"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..2033
                     /locus_tag="PLG98_11885"
     CDS             129..2033
                     /locus_tag="PLG98_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOE34010.1"
                     /translation="MTLNPQLKNPKFYLMVVMDILLFITALFSSYLIRFEFNLAAIDL
                     KQVYSLLLWMVPLKFIIFLAVGLYHGMWRYTSVKDFWLLARACFLSMALILLILLVIN
                     RFQGYSRAIMIADGIMTFLLTGGIRLVIRSFYARRVNQLTNISLPFNVRLTKVLIIGA
                     GQAGEKILREIMDNYTLPYEVVGFIDDDPGKQGLTIHGIRVLGTVECLPEIINRESIQ
                     QIFIAVPSASGEQIRRIVEVCQQCNTSYKILPGIGALIDGRVSVKLLRDISYEDLLGR
                     SPVHLDIKGIRNYLDGKTILITGCGGSIGSELCRQVVKYQPHNLILMDSSEANLFQIQ
                     MEMQNENYCRHCEAVLGRVQDEALMNSVFEKYKPEVVFHAAAYKHVPMMEKNPWQAVF
                     NNVVGSRVVMEMAVKHHVERFVLVSTDKAVRPTNVMGASKRVTELIMQCQQGNGTKFM
                     AVRFGNVVGSSGSVIPFFRRQIEQGGPVTVTHPEVNRFFMTIPEASQMILQAGTMGEG
                     GEIFILRMGTPVKIVDMARDLIRLSGKEPDVDIKIVYTGLREGEKLYEELITAGEDIL
                     PTGHKKVMVLRSNGYFNGAGNPQEAKEYLYREIDELVTIADRHDSKGIKKKLQEIVSE
                     FAPQENESVL"
     gene            2104..>2910
                     /gene="rfbB"
                     /locus_tag="PLG98_11890"
     CDS             2104..>2910
                     /gene="rfbB"
                     /locus_tag="PLG98_11890"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOE34011.1"
                     /translation="MNKILVTGGCGFIGSNFVRHTLQHYPDFRIINLDKLTYAGNPGN
                     LTDIEKNPRYRFVKGDIGDAVLVRQIFAEEQIDTVVHFAAESHVDRSITGPAEFVQTN
                     IMGTFNLLEAAREHWLKDSADAAKKTCRFLHVSTDEVYGSLGETGAFQETTPYDPRSP
                     YSASKASSDHLVSAYFHTYGLPTLITNCSNNYGPYQFPEKLIPLIINNALQGKSLPVY
                     GDGKNVRDWLYVVDHCEAILTVLQKGKIGETYNIGGNSEKQNIEIVNLICD"
BASE COUNT          747 a          700 c          739 g          724 t
ORIGIN      
        1 cggggacggc tgccgtccgt tacattccgc aatcgcaatg gctttttaac aacacacttg
       61 ttttcaaaga gtatatcggt atgttctggt acagcatcaa agatatataa ggagtcggat
      121 aaaatactat gaccttgaat ccccagttaa aaaatcctaa attctattta atggtagtga
      181 tggatatttt gcttttcatc actgcacttt tttcatccta tctgattcgc tttgaattta
      241 accttgctgc aattgacctc aagcaggttt acagcctgct cctctggatg gtccccctga
      301 agtttatcat cttccttgct gtcgggcttt accacggcat gtggcgatac accagcgtga
      361 aggacttctg gctgctggcc cgggcctgtt tcctgtcaat ggcgctcatt ctgctgattc
      421 tgctggttat caacagattt caaggatatt cccgcgccat aatgatcgcc gacgggatta
      481 tgaccttcct gctgaccggc ggcatccgcc tggtaattcg ttccttttat gcgaggcgtg
      541 tgaatcagct gaccaatatt tctctgccgt tcaatgtccg gttgacaaaa gtattgatta
      601 tcggcgccgg gcaggcagga gaaaaaatat tgcgggaaat catggataac tacaccctgc
      661 cgtatgaagt tgtcggcttt attgatgacg atccgggaaa acagggattg accatccatg
      721 gaatacgggt attgggcacg gtcgagtgct tgcctgaaat tatcaacagg gaaagcatcc
      781 agcaaatatt tattgcggtt ccttccgcta gcggcgagca aatcaggcgg attgtggaag
      841 tctgccaaca atgcaatacc tcttataaaa tattgcccgg catcggcgcg ttgattgacg
      901 gccgggtcag cgtgaaactg ctccgggaca tcagttacga agatctgttg ggacggtcgc
      961 cggttcatct ggatattaaa ggcatccgca attatctgga tggcaaaacc attctgatca
     1021 ccggttgcgg cggctccatc ggctctgagt tatgcaggca ggtcgttaaa taccagcctc
     1081 acaaccttat cctgatggat tcgagtgagg ccaatctgtt ccaaatccag atggaaatgc
     1141 aaaatgaaaa ttattgccgc cactgtgagg ccgttctggg acgggtgcag gatgaagcgc
     1201 ttatgaatag tgtgtttgaa aaatacaaac ccgaagttgt gtttcacgcg gcggcttata
     1261 agcatgtgcc catgatggag aaaaatccct ggcaggccgt tttcaataac gtggtcggca
     1321 gccgcgtggt tatggaaatg gcggtcaagc atcatgtgga gcgatttgtt ctggtttcga
     1381 ccgataaggc cgttcgtccg accaacgtca tgggcgccag caagcgggtg acggaattga
     1441 tcatgcaatg ccagcaggga aacggcacga agttcatggc cgtgcgattt ggcaatgttg
     1501 tcggttcgtc cgggtccgtc attccctttt tccgccgtca gatcgagcag ggcgggccgg
     1561 tgaccgtcac gcacccggag gtgaaccgtt tcttcatgac catacccgaa gcgtcgcaga
     1621 tgatcctgca ggccggcacg atgggagaag gcggcgaaat ctttattttg agaatgggaa
     1681 cgcccgtcaa aatcgttgac atggctcgcg acctgatccg cctttccggt aaagaaccgg
     1741 atgtggacat caaaattgtc tataccggtt tgcgggaagg tgaaaagcta tacgaagagc
     1801 tgatcacggc aggagaggat attctgccca ccggccacaa aaaggtcatg gttttgcggt
     1861 ccaacggtta tttcaacggc gcgggcaatc cgcaggaagc gaaagaatat ctttatcgtg
     1921 aaattgatga attagtgaca atagcggacc gtcacgactc aaaaggcata aagaaaaaac
     1981 ttcaggaaat tgtatccgaa tttgcaccac aggaaaacga aagcgttctg taaatagctc
     2041 atggctcgta gctgatagct catagtgatt gcggggtaac aactttaaac aggagtaaca
     2101 aatatgaata aaatacttgt aacaggcgga tgcggattta tcggatcaaa ttttgtccgc
     2161 cacaccctgc agcattatcc ggactttcgc atcattaatc tggataagct gacctacgcc
     2221 ggcaatcccg gcaatttaac ggatattgaa aagaatcccc ggtatcgctt tgtgaagggt
     2281 gatatcggcg atgccgtttt agtcaggcaa atctttgccg aagaacaaat agacaccgtt
     2341 gtccattttg ccgccgagtc ccatgttgac cgcagcatca ccggcccggc tgaatttgtc
     2401 caaactaata ttatgggaac cttcaatcta ttggaagcgg cgagagagca ttggctgaaa
     2461 gattcagcag acgcggcaaa aaaaacctgc cgttttctgc atgtcagcac ggacgaggtt
     2521 tacggctctc tgggcgaaac cggcgctttt caggagacta caccctatga tccccggtcg
     2581 ccttattccg ccagcaaggc gtcctccgac catctggtca gtgcgtattt ccatacctat
     2641 gggctgccga cgctgattac caactgctcc aacaactatg gcccgtatca gtttccggaa
     2701 aagcttattc ctctgattat caacaacgcc cttcagggaa agtctttgcc ggtttatggc
     2761 gacggcaaaa atgtccgcga ctggctttat gtcgtcgacc actgcgaggc cattctgacc
     2821 gtcctgcaaa aaggcaaaat cggtgagacg tacaatatcg gcggcaacag cgagaagcag
     2881 aacatcgaaa tcgtcaacct catctgcgac
//
LOCUS       DAOSME010000170         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_37408_length_2896_cov_21.500528, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000170 DAOSME010000000
VERSION     DAOSME010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37408_length_2896_cov_21.500528"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1431)
                     /locus_tag="PLG98_11895"
     CDS             complement(259..1431)
                     /locus_tag="PLG98_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOE34012.1"
                     /translation="MKIQDIKTIAVIGAGDMGHGIAQVCAMAGMKINLYDIKQEFVDR
                     GMAKMKASFEKQVAKQKMSVDDLNKMMGNINGFISLKDAVADIDYMIEAAPEILDLKI
                     KIFKEVDENAPKHAILASNTSNMSITEMGAATKRPDKVLGIHFFNPPVMMALVEVIRG
                     KQTSLETMNVSFELMSKLKNFRGAMVPVRVEKDSPGFIFNRINAPVGLYLAQIYEQGL
                     VKPEAVDAKIRSIGSPMGPYETMDFSGLDVYMHGTEYFAKTLSPEFKPTNWLKKMNET
                     GTLGKKSGKGIFVWPEGNRPQIDMSKADPNFDPMDIVCLQVNEGTKLLEEGVTGSAAE
                     IDKAMVNGGGSPFGPFALAKGMGWPQVAERCEAISKKLGIQWFLPTETLKKGNIQV"
     gene            1669..>2896
                     /locus_tag="PLG98_11900"
     CDS             1669..>2896
                     /locus_tag="PLG98_11900"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOE34013.1"
                     /translation="MNDFKNNREHWATRAGFILAAAGSAIGLGNIWRFPYMTGEHGGA
                     AFIVIYLIAICLIGYPLIVNETVLGRVSQRNPVGAFKALAPNSPWWLVGALGVFTGFV
                     ILSYYVVVAGWSLAYVYKVIVATGSPDINHAEIFKNHIASIWEPICWQALFMILTIGI
                     IAAGVVKGIQRWSTILMPVLFIILLILILRSVTLPGASEGIIYYLKPDFSEVTGRTLL
                     GAISQAFFSLSLGMGVFITYGSYLQSKDEIPFSAASVAGLDTAVALLAGFAIFPAVFA
                     LGYAPGAGPGLVFITLPAVFAQMPLGIVFGVLFFVLLSIAALTSAISLLEVVSAWLID
                     EKGWQRKKAAICMGLVIFIAGLPAGLGYNVLDGVHCPGLKTDLLDTYDWFANSIFLPL
                     GGLLSAIFVGYIWGTKK"
BASE COUNT          637 a          639 c          699 g          921 t
ORIGIN      
        1 taagtcaaaa ttgattgttt ttgttttgtg aaaaatgggc aaagaaaaag gatatgcttt
       61 ttctgtcgga tatctttcgg ggtggaaggt cacgctgttt atatgcattt agcactctga
      121 acgaaaaact ttaatctgcc gcaatttata tatcaataaa tacgcgatta caagaaagtt
      181 gtgtcaccgg ttgcgggatt agaaaatagg gagttcatca ctttgatgaa ctccctatcg
      241 tgattttcgg aaacttcttt aaacctgaat atttcctttc ttcagtgttt cggtgggaag
      301 aaaccattgg atacccagtt tcttagagat agcttcgcag cgctcggcaa cttggggcca
      361 gcccatacct ttagctaggg caaaaggccc gaagggagaa ccgccgccgt tgaccatggc
      421 tttgtcgatt tccgctgcgc tgccggtcac gccttcttcc aaaagttttg ttccctcatt
      481 tacctgcagg cagacgatat ccatcggatc aaaattagga tcagccttgc tcatgtcaat
      541 ttgcggcctg ttgccttccg gccagacgaa tattcccttg ccgctttttt tgcccagtgt
      601 cccggtttca ttcatttttt tgagccagtt ggtgggcttg aattcaggag agagggtttt
      661 ggcaaaatat tccgtgccat gcatataaac atccagcccg gaaaaatcca tcgtttcgta
      721 aggtcccatc ggagaaccaa tagatctgat tttggcgtcg accgcttccg gttttaccaa
      781 accttgttcg taaatctggg ccagataaag gcctaccggc gcgttgatcc gattgaagat
      841 aaagcccgga gaatcttttt ccacccttac cggaaccatc gcgccgcgga agtttttcaa
      901 ttttgacatc agttcgaagc tgacgttcat ggtttcgagg gatgtttgct ttcctctgat
      961 gacctcgacg agtgccatca tgactggcgg gttgaaaaag tgaattccca aaactttatc
     1021 cggtctcttt gtcgccgcgc ccatctccgt gatgctcata ttggatgtat tggaggccaa
     1081 gatagcgtgt tttggcgcgt tttcgtcaac ttctttgaag atttttattt taaggtcgag
     1141 tatctccggc gcggcctcaa tcatataatc gatgtcggcg acggcatctt tcaaagagat
     1201 gaagccgttg atgtttccca tcattttgtt caaatcgtcc acggacattt tttgcttggc
     1261 cacctgcttt tcaaagctgg ctttcatttt tgccatgccc ctgtccacaa attcctgctt
     1321 gatgtcgtaa agattaattt tcatgccggc catagcgcag acctgagcta tgccgtgacc
     1381 catgtcgccc gcgccaatga cggcgattgt cttgatgtcc tgaattttca tagatggcac
     1441 tcctttcttt tttattttta caatgtcgtg attttaattt aagaggttta tgaatatata
     1501 atgaaaatat taaagctgtg tcaagttaat taagatattg gaaaaaacgc gcttgtccgg
     1561 gaaatgtttt caatatgtgt tttgtcttga caccaggaaa tgaaatttct atagttcgcg
     1621 tcgaaaaatt tatttgccct tgttttgatt ttaaagagga gaaacacgtt gaacgatttc
     1681 aaaaacaaca gagaacattg ggcgactcgt gccggattca ttttggcggc ggcaggttcg
     1741 gcaataggtc tggggaatat ctggcggttc ccgtacatga cgggtgagca tggcggcgcg
     1801 gccttcattg ttatttacct gattgccatc tgtttgattg gctatccatt aattgttaat
     1861 gaaacagtcc ttggtcgtgt ttcccagcgg aatcctgtgg gagcctttaa agctctcgcg
     1921 cccaacagcc cctggtggct cgtaggcgcg ttaggtgttt ttaccggctt tgtcattctt
     1981 tcctactatg ttgttgttgc tggctggtct ttggcttatg tttataaagt catcgtcgca
     2041 acaggcagcc cggatattaa tcacgccgaa attttcaaga atcatatagc aagcatttgg
     2101 gaaccgattt gctggcaggc tctgtttatg attttaacca tcggcattat tgccgccggc
     2161 gtggttaagg ggatacaaag atggtccact atccttatgc cggtactttt catcatattg
     2221 ctcattttga ttctgcgttc tgtcaccctg cccggggcga gtgagggtat tatctattat
     2281 ctgaaaccgg atttcagcga ggtgaccggt aggacgcttc ttggcgctat ttcccaggct
     2341 tttttttctc tgagtttggg catgggtgtt ttcattacct acggaagtta tctgcaaagc
     2401 aaagatgaaa ttccattcag cgccgcttct gtggccggtt tggacactgc ggtcgccttg
     2461 ttggctggtt ttgccatatt tccggctgtg tttgctttgg gatatgctcc gggagcgggc
     2521 ccgggtctgg tttttattac gctccccgct gtttttgcgc aaatgcctct ggggattgta
     2581 tttggcgttc ttttttttgt tttgctgagc attgccgctt tgacttccgc tatttctctg
     2641 ttagaggttg tatcggcctg gctgattgat gaaaaaggat ggcagcgtaa aaaagccgca
     2701 atctgcatgg ggctggtaat ttttatcgcc gggctcccgg ctggtttagg ctataacgta
     2761 cttgacggag ttcactgtcc cggtctcaag acggatctgc tcgataccta tgactggttt
     2821 gccaacagca ttttcctgcc gctgggggga ctgctgtcgg caatatttgt cggctacatc
     2881 tgggggacaa aaaaag
//
LOCUS       DAOSME010000171         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_37952_length_2862_cov_18.736730, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000171 DAOSME010000000
VERSION     DAOSME010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37952_length_2862_cov_18.736730"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..2648
                     /locus_tag="PLG98_11905"
     CDS             228..2648
                     /locus_tag="PLG98_11905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE34014.1"
                     /translation="MKISNSSKGFAVIVILLLALIGFAVFKALGIQENIILSEQHRLR
                     SFRLAIELFQSSEDLTRMARSYVSTADPTYERRYFEILDIRNGARPRPANYNATYWHL
                     AGAGRGGAVAPGETIALQELMRREGFSEKEFSLLRESQANSDHLVHMEKQAFAAMKGL
                     YDDGRGRFTVRRAPDRKYAVNLLFSEQYSNEKARIMLPIQYFMDEINKRTKTHLNDLQ
                     SELFRIIVAVLALVLITLLVVLIHTFRTFRHILSPTKRLLGKINEIASGNYAARCPVS
                     SSNEIGELSSKFNYMADALQTDVLRREEVEKNLKESEAQVRLLLNSTAEAIYGIDLQG
                     NCTFANQACIRMLGYADIQQLLGKNMHNLIHHSYPDGRTMPNETCRIYSAFRQGKGVH
                     VDDEVLWKADGDSFPAEYQSFPQIEDGRVIGAVVTFTDITERKRVEKYREMGNEVLNV
                     LNNPDFQQDVMQGVVDVLKSRTGFDAVGIRLRKGDDFPYFAQSGFSDSFLQTENSLVN
                     RDAVGGLCRDQNGRILLECNCGLVLSDRTQPDCPCFTRFGSFWTNDSSPLADLPSDQD
                     PRVSPRNQCLRQGYASLALVPIREKGSIIGLIQFADRKKNCFSIAEIEQMENVAANIG
                     EALLRREAEEQIRHMATHDGLTDLPTMRLATDRLSMSILRARRNKTQTAVMFVDLDGF
                     KTVNDTLGHEAGDYVLKEIAGRLLSCVRESDTVARVGGDEFLVIAGGIHAPENASRIA
                     ENILRSVPRPVLFNGSPAVVGASIGIALYPDNGDDMEQLIRASDQAMYRVKKGGKNGF
                     CFADATAK"
BASE COUNT          706 a          721 c          792 g          643 t
ORIGIN      
        1 cgggaatatc gaacgggcga aacagaaggg ctggcaaacc atccattacg cggatgaagc
       61 tctttttttt gcggaaataa accggctgcc gggccttgaa acgcgggaag aacgcggata
      121 ggaaaaagga agctccgttt gcctgttgac actcaaaaaa acccgggata tgctcaaaat
      181 gactgaagac ggaatcgaag gatgtctgat caagggatgc gatttccatg aaaatcagca
      241 attcaagcaa gggattcgct gttatcgtga tcctgctact ggcgctgatc ggatttgctg
      301 ttttcaaagc gctagggatt caggaaaata ttatcctcag tgaacagcat cgtctgcggt
      361 catttcggtt ggccattgaa ctgtttcaaa gctcggaaga tctgacccgc atggcgcgca
      421 gctatgtctc gacggccgat cccacatacg agagacgcta tttcgaaatc ctggatatcc
      481 gcaatggtgc acggcctcgg ccggcaaatt acaacgcgac ctactggcat cttgccggcg
      541 caggaagggg cggagccgtc gcgccggggg aaaccatagc ccttcaggaa ttgatgcggc
      601 gggagggctt ttcggaaaag gaattcagcc tgctgcggga gtctcaggca aattcagatc
      661 acctggtcca tatggaaaaa caggcttttg ccgccatgaa gggcttgtat gacgatggcc
      721 gtggccgttt caccgtgcgc cgggcgccgg accgcaagta tgcggtcaat ctgctgttca
      781 gcgaacagta ttcaaatgaa aaagcccgga tcatgctgcc gattcagtat ttcatggatg
      841 aaatcaataa gagaaccaaa acccatttaa acgatcttca gtctgaactt ttcaggatca
      901 ttgtggcggt gctggctctc gtcctgatca cgctgctggt ggtcctgatc catacctttc
      961 gcacgtttcg gcacatctta tccccgacaa aacgtttgct cggcaagatc aatgaaatcg
     1021 catccggcaa ttacgcggcg cgttgccctg tatcatcctc caacgagatc ggcgaattgt
     1081 catcaaaatt caattatatg gcagatgctt tacaaacgga cgtcctcagg cgggaggagg
     1141 tggaaaaaaa tctgaaggaa agcgaggcgc aggttcggct tttgctcaat tcaaccgcgg
     1201 aggcaattta cggcatcgat ctgcagggca actgcacctt tgccaatcaa gcctgcattc
     1261 gtatgctggg gtatgcggat attcaacaac tgctcggaaa aaacatgcac aatctgatcc
     1321 atcattccta tccggacggc aggacgatgc ctaatgaaac atgccggata tatagcgcct
     1381 tccgtcaggg caagggtgta cacgttgacg atgaggtgtt gtggaaagcg gacggcgaca
     1441 gttttcccgc ggaataccag tcttttccac aaatcgagga cggccgggtt atcggagccg
     1501 tggtgacgtt cacggatatt accgaacgaa aacgcgttga aaaataccgc gagatgggca
     1561 atgaagtcct gaacgttctc aacaatcccg actttcagca ggatgtgatg cagggcgtcg
     1621 tcgatgtgct gaaatcgcga accggcttcg acgcggtggg aatccgcctg cggaaaggtg
     1681 atgactttcc atattttgcc caaagcggtt tttccgattc ttttctgcag actgaaaatt
     1741 cgctggtcaa ccgggacgcc gtcggcggac tgtgccggga tcagaacggc cggattcttt
     1801 tggagtgcaa ctgcgggctg gtcctttccg accggacgca gccggactgt ccatgcttca
     1861 cccggtttgg cagtttctgg accaacgatt cctctccgtt agcggatctt ccgtccgatc
     1921 aggacccgag agtttctccg cgcaatcaat gcctgcggca gggctatgcc tccctggcgc
     1981 tggtgcccat tcgggaaaag ggatccatta tcggcctgat ccagtttgcc gaccggaaga
     2041 aaaattgttt ttccatcgct gaaatcgaac agatggaaaa cgttgcggcc aatatcggcg
     2101 aggccctgct gcgcagagaa gcggaagagc agatccggca catggcaact cacgacggct
     2161 tgacggacct gcccactatg aggctggcca cagaccgcct gtccatgtcc atcctccggg
     2221 cgcgcagaaa caagacgcag acggccgtca tgtttgtgga tctggacggc ttcaaaaccg
     2281 tcaacgacac cctggggcat gaagcggggg attatgtcct caaggaaatt gccggtcgtc
     2341 ttttgtcctg tgtacgggag agcgacacgg ttgcgcgggt tggaggggac gagtttctgg
     2401 tcatcgcggg cggcattcat gcccccgaaa acgcatcccg gatcgccgaa aacatcctcc
     2461 gctcggttcc ccgccctgtt cttttcaacg ggagtccggc cgtggtcggc gccagcatcg
     2521 ggatcgcgct atatcccgac aacggcgacg atatggaaca gctgatcagg gcgtctgatc
     2581 aagccatgta tcgcgtcaaa aaaggaggta agaatggttt ctgctttgcc gatgccacgg
     2641 caaaataacc ttatcatgcg accagttcag aaagggattt cggatgatat gaatgcgggc
     2701 tgaaggtttg ccttgacaaa ccctttgagt cattttaaat ttcacaagca aaaaattgga
     2761 ttttccataa gcgacaggcc gtcatcgcaa caaaagaaag ggagaagagc aaatgaaaaa
     2821 aatcgcaggt tatgttgttt cgttgttttt gctgtttgct gt
//
LOCUS       DAOSME010000172         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_38323_length_2841_cov_16.703877, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000172 DAOSME010000000
VERSION     DAOSME010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38323_length_2841_cov_16.703877"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLG98_11910"
     CDS             <1..222
                     /locus_tag="PLG98_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34015.1"
                     /translation="MMSGWKTAKQLAFMANGFHAIRVATEDGDTKEGVLPVGQVMGLL
                     HDEPTVAELMERMAVEAEKQQQKLNAHFA"
     gene            445..1194
                     /locus_tag="PLG98_11915"
     CDS             445..1194
                     /locus_tag="PLG98_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE34016.1"
                     /translation="MYQGKKVVVVMPAYNAEKTIIKTYNEVMAQGVVDKVILVDDASP
                     DNTVKVAERLPATKVFVHERNLGYGGNQKTCYRLALEDGADIIIMVHPDYQYTPRLIP
                     AMTSMIGNGLYHCVLGSRILGGYALKGGMPYWKYIANRFLTFTENILIGAKLSEYHTG
                     YRAFSRDLLLKLPLDKNSDDFVFDNQMLAQIVWFGYTVAEVSCPTSYFAEASSINFSR
                     SVKYGFGCLSTAFAFRLAKMKLIKNNLFPET"
     gene            complement(1273..1932)
                     /gene="rpe"
                     /locus_tag="PLG98_11920"
     CDS             complement(1273..1932)
                     /gene="rpe"
                     /locus_tag="PLG98_11920"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOE34017.1"
                     /translation="MKLIAPSILSADGSKLGEEIIAVEKAGADWIHVDVMDGNFVPNI
                     TMGPSIISNLRKVTKLPFDVHLMIADPDRYIESFFKAGADIITVHVEAAGHLHRTIDL
                     IKSYGARAGVSLNPATPLDQVSEIVHYIDLLLIMTVNPGFGGQQFIGTSLAKIARARK
                     MLDELPHQPLLEVDGGVNLKNIGDIAQAGTDVIVAGNAVFGSGDYEKNIAALKTIANS
                     R"
     gene            2064..>2841
                     /locus_tag="PLG98_11925"
     CDS             2064..>2841
                     /locus_tag="PLG98_11925"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOE34018.1"
                     /translation="MYILYNIILFVAALIAAPYFLLKIIFTGKYRNSFIQKLGGKQKA
                     ILGRLKNKPRVWIHAVSVGEVTAAAPIVASLKKKRPDVDVIFSTSTETGQDMARKFIK
                     DASALIYFPLDFPPVVGKMIKSAAPDVFVLVETELWPNFLRGCDRRGISALMVNGRIS
                     PRSYRRYRLTRFLWKGVLKDLDFAGMISSVDATRLESIGMDPAKITVLGNAKYDSLAS
                     MVSPVLQEEMARRLNVTAQEKFFVAGSTHPGEEKIIIHVYR"
BASE COUNT          689 a          678 c          744 g          730 t
ORIGIN      
        1 atgatgtcgg gttggaaaac cgccaagcaa ctggcgttca tggccaacgg tttccacgca
       61 atccgggtcg ccaccgaaga cggggatacg aaggaaggcg ttcttcccgt gggtcaggtg
      121 atgggcttgc tgcacgatga gccgaccgtg gccgaactga tggaaagaat ggcggtggag
      181 gcagaaaagc agcagcagaa acttaacgct catttcgcgt agcaatggtt tcccccgctg
      241 tttgtcatgg tctccggctt gaccgtttca tgcaggtata tgtgtggcgc gttcggcaga
      301 acgcgcccct ttgtctcaga tgttgtttcg aaccgctgaa agcgagtaag gaatctcagg
      361 tttctctcgt cgctttgctc ctcgacatga cgcaaaaccg cgttatgcga atttctcata
      421 accggagaat tggttgggca gggaatgtat cagggcaaaa aagtcgtggt ggtgatgccg
      481 gcttataatg ccgaaaaaac cattatcaaa acatacaatg aagtcatggc gcagggtgtc
      541 gtcgacaagg tgatcctggt tgacgacgcc agcccggaca ataccgtaaa agtagccgag
      601 aggcttccgg caacgaaagt tttcgttcat gagcgcaatc tgggctatgg cggcaatcaa
      661 aagacctgct accgtctcgc tcttgaagac ggcgccgata tcatcatcat ggtacatccc
      721 gattatcagt acacgcccag gctgattccg gcgatgacgt ccatgatcgg caacggtctt
      781 tatcattgcg tgctgggttc acgcatcctg ggcggttacg ctctcaaagg cggcatgccg
      841 tactggaaat acatcgccaa ccgtttttta accttcacag aaaacatttt aattggcgca
      901 aagctttccg aataccacac cggttaccgc gctttttccc gcgatcttct gttgaagctt
      961 cctcttgata aaaattccga tgatttcgtg tttgataatc agatgctggc gcagatcgtc
     1021 tggttcggtt ataccgttgc ggaagtgagt tgtccgacca gctattttgc cgaggcctca
     1081 tccattaatt tcagccgaag cgtcaagtac ggcttcgggt gcctgagcac agcgtttgct
     1141 tttcgtctgg caaagatgaa attgatcaaa aacaaccttt tcccggaaac gtaaacagta
     1201 ggaagtgaaa tgtgaaatgt gaaacgtgaa ggaataatct tttcactatt cacccttcac
     1261 tcttcacgtt ttttagcggc tgttggcgat ggttttcaat gcagcgatgt ttttctcata
     1321 atctccgctt ccaaagacag cgttgccggc gacgatgacg tcggttccag cttgagcgat
     1381 atcgccgata tttttcaggt ttacaccccc gtcaacttcc agcagcggct gatgcggcag
     1441 ttcatccagc atttttcttg cacgggcgat tttagccaga gaggttccga taaattgctg
     1501 gccgccgaat ccggggttaa ccgtcatgat caacagaaga tcaatatagt gaacgatttc
     1561 tgaaacctga tccagcggcg ttgcgggatt cagagaaaca ccggcccgtg cgccgtagct
     1621 tttaatcagg tcgatggtcc gatgcagatg gccggccgct tccacatgaa cggtaataat
     1681 atcggcgccc gccttgaaaa aggattcgat gtagcgatca gggtccgcaa tcatcaaatg
     1741 gacgtcgaag ggcagtttgg tgacttttct cagattggag ataatggatg gtcccatggt
     1801 aatgttgggc acaaaattgc cgtccatcac atccacgtgg atccagtcgg ctccggcttt
     1861 ttccacggcg atgatttcct cgcccagttt gctgccgtcc gcggaaagaa ttgaaggggc
     1921 aattaatttc attttttctc tccccgagaa ttaatatacg ctaaattata ataaagagaa
     1981 aagtttgtca atgaatgcat tttttatgct ttgaagggcg gatagtgctt gacacccgtg
     2041 cggaaaaatc taaaaatgca ttcatgtaca ttctttataa tattattttg tttgttgcgg
     2101 ctttgattgc cgctccttat tttctgttga agattatttt tacaggcaag taccgcaaca
     2161 gctttattca gaaactcggg ggaaagcaaa aggccatcct gggcagactg aaaaacaagc
     2221 cgcgggtctg gattcacgcg gtttccgttg gcgaggtgac ggccgctgcg ccgattgtgg
     2281 catctttgaa aaagaaaaga ccggacgtgg atgtcatatt ttcgacgtcc acggaaacag
     2341 gtcaggacat ggccagaaag ttcatcaaag acgcttcggc gttgatttat tttcctctgg
     2401 attttcctcc ggtggtcggc aaaatgatta aatcggcggc gcccgacgtt ttcgtcctgg
     2461 tggaaacgga gctctggccc aattttttgc gaggctgcga tcgtcgcgga atcagcgcgc
     2521 tgatggtcaa cggccgcata tcgccgcggt cataccgacg gtaccgcctc acccgctttt
     2581 tatggaaagg cgttttgaag gatcttgatt ttgcggggat gatttccagc gtcgatgcga
     2641 caagattgga gagcatcgga atggatccgg cgaaaataac ggtgctgggc aatgccaaat
     2701 acgacagcct ggcctccatg gtctcgcccg tgttacagga agaaatggcc cgtcgcttaa
     2761 acgtcaccgc gcaggagaaa ttttttgtcg cgggcagcac gcatccgggc gaggaaaaaa
     2821 tcatcattca tgtctaccgt g
//
LOCUS       DAOSME010000173         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_39804_length_2760_cov_28.080961, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000173 DAOSME010000000
VERSION     DAOSME010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39804_length_2760_cov_28.080961"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>2760)
                     /locus_tag="PLG98_11930"
     CDS             complement(1..>2760)
                     /locus_tag="PLG98_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE34019.1"
                     /translation="YKGKACEIVGDKEVFGQRIAWIRLMVDGSFHEVPYDELEKPTTE
                     FSMPYLRFVSIAAKIKDEVARKNLLAPYESSLIPLPHQILVLEKVMQSNQNRFLLADE
                     VGMGKTIETGLILKELKIRGDIKRILCVVPKSAMLQWQSELKEHFNEVFHLYDSEMIT
                     SMARTFANINADEEYNFWTQHNQIIVSTDALKPLEKRQGWSNERVDEYNKYRMQAVLE
                     ADFDLVIMDEAHKMGGATSLVSRFILAQELCNSVPNVLLLTATPHRGKSDHFRRILQL
                     LDPDAFAGEGLPDIEELEPYVIRTERRFAVDYEGKKLFNERQTRRFDVMLDINKHQKQ
                     MALYDAVTDYVRRGFNTAKRAGNPAKGLIMILFQRLVSSSTAAILSAMQGRLERLRLG
                     EESTLDDFAHEVDHEFDENEEVDFEDMVQRNALGLASEEALLSDLIAQARDCLATETD
                     AKADALLKKIEELKQVQITQELKILVFTEFRTTLRMLVDYFKGKGYVVSYIHGGQDLD
                     ERKRALTQFKNESQILIATDAAGESLNMQFCHIVFNYDLPWNPMMIEQRIGRVDRIGQ
                     KYPVQAHNMLTNNSVDLRVYEVIEEKLNNILEQLGIDKTSDVLDSTIDMKKVNKLHLQ
                     SLLDPERFEFAGERWLHDIKSKLRQYKSTEGILPQVAESEIEYKKAAEVKHSPLPFWL
                     ETLVQQYTMVHHGTLTKNLLGYADIKVQGESRKITFDAEVSLGNPGVEHITLQHDWIR
                     KLLNDLGEFDLSNKIPVIKSSTGEETPGYWSLWQISARNNLESKIQYQCYFLADNGKQ
                     YSAYAHDIWNRLINGSGKFSIDGSQAFNEPESFQKEMNQSLFLSFQNLEADVMIRINT
                     KKENRLNSYCFQKSRIERIGIDNIRQAKLQRLEKEHQAWMKEFDSGSKIIPGIKQLLM
                     IRING"
BASE COUNT          698 a          681 c          519 g          862 t
ORIGIN      
        1 ttagccattg atacgaatca ttaatagttg tttaatgccc ggaatgattt tgctccccga
       61 atcaaattct ttcatccatg cttgatgctc tttttcaagt ctttgaagtt tggcttggcg
      121 aatgttgtcg attccgatcc tttcaattct ggatttctga aaacaatagg aattcaacct
      181 gttttccttt ttggtgttaa ttcgaatcat tacatctgct tcaagattct ggaatgaaag
      241 aaacaaggat tgattcatct ctttttggaa cgactccggt tcattgaacg cctgtgagcc
      301 atctatgcta aattttccag atccgttgat caaacgattc cagatatcat gggcataagc
      361 actgtattgc tttccattat cggctaaaaa gtagcattga tactgaatct tactttccag
      421 gttattgcgg gcacttattt gccacaaact ccaatagccc ggagtttctt ctccggtgct
      481 tgacttgatt acaggaattt tatttgacag atcaaattcg cccagatcat ttaatagttt
      541 cctgatccag tcgtgctgca gcgtaatatg ctcaacgcca ggattaccta atgaaacctc
      601 ggcatcaaag gtgatcttcc tgctttcgcc ctgtacttta atgtcagcat accccaatag
      661 attttttgtc aaggtgccat gatgcaccat ggtgtactgt tgcaccaaag tttccagcca
      721 aaaaggaagc gggctgtgtt ttacctctgc cgcctttttg tattcaattt ccgattctgc
      781 tacttgtggc aatatgccct cggtgctttt atactggcgc agcttactct ttatgtcatg
      841 caaccatcgt tccccagcaa attcaaatct ttccggatct aacaaactct gcaggtgtag
      901 tttatttact ttcttcatat cgatggtaga atcgagcaca tccgaagttt tgtcaatgcc
      961 caactgctcc aaaatgttgt tgagcttttc ttcaatcacc tcatatacac gcaagtcaac
     1021 actattgttg gtgagcatat tatgggcctg gaccggatat ttttgaccaa tcctatcaac
     1081 ccggccaatg cgttgttcaa tcatcatggg attccagggc aagtcataat taaacacaat
     1141 atgacaaaac tgcatgttca gggattctcc tgcagcatcg gtagcaatca ggatttgcga
     1201 ttcgttttta aattgtgtta aggctctttt tcgctcatcc aaatcctgtc cgccatgaat
     1261 ataagagact acgtaccctt tccctttgaa atagtccacc aacattctca gtgtggttct
     1321 gaactctgta aagaccagta ttttcaattc ctgagttatc tgaacctgct ttaattcctc
     1381 gattttcttc agaagcgcat cggctttggc atcggtttct gttgccaaac aatctcttgc
     1441 ctgcgctata aggtctgaca gcaacgcttc ttcactggct aagccgagcg cattgcgctg
     1501 taccatgtct tcaaaatcta cctcttcatt ttcatcaaac tcatgatcta cttcgtgcgc
     1561 aaaatcgtcc agagttgatt cttcgcctaa tcgcaatcgt tccagcctgc cttgcatggc
     1621 agaaagtata gcggctgtgg aactgctcac caaacgttga aacaagatca tgatcaatcc
     1681 ttttgcagga ttaccagccc ttttagccgt gttaaatccc cttcgtacat aatccgtaac
     1741 ggcatcatac aaggccattt gtttttgatg cttgttgata tccaacataa catcaaagcg
     1801 cctcgtctgc ctttcattaa agagcttttt cccttcataa tccacagcaa aacgcctctc
     1861 ggttcggatg acataaggct ccagttcctc aatatcgggc aacccttcac ccgcaaatgc
     1921 atccgggtct aatagctgca atattcttct gaaatggtct gatttgcccc tgtggggcgt
     1981 tgccgtaagc aacagcacat tcggcacaga attgcataat tcctgcgcca ggataaaacg
     2041 tgaaaccagg gatgttgcgc cgcccatttt atgggcttca tccataatga ccaagtcaaa
     2101 atctgcttcc agcaccgcct gcatgcggta tttgttgtat tcatccaccc gctcatttga
     2161 ccagccttgc cttttctcca aaggcttcag cgcatcggtt gaaacaataa tttggttgtg
     2221 ctgtgtccag aaattgtatt cttcatcggc attgatattg gcaaaagtgc gtgccatgga
     2281 ggtaatcatc tccgaatcgt agagatgaaa cacttcattg aaatgctctt tcaactcact
     2341 ctgccattgc agcatagcag actttggaac aacacacaat atgcgcttga tatcgcctct
     2401 gattttaagc tccttcaaaa tcaaaccggt ctcaatggtc tttcccatgc ccacctcatc
     2461 ggccaacaaa aatcgatttt gattggactg catcactttt tccagcacca gtatctgatg
     2521 cggcaagggg atcaaactgc tctcatatgg agccaatagg ttttttctgg ctacttcgtc
     2581 tttaattttt gccgcaatgg atacaaagcg taagtagggc atggaaaact ccgtagtggg
     2641 tttttccaac tcatcatagg gaacttcatg gaagctgccg tccaccatca ggcgtatcca
     2701 ggcaatgcgt tgcccgaaca cttctttatc tcctactatt tcacaagcct tgcctttgta
//
LOCUS       DAOSME010000174         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_40288_length_2736_cov_20.728460, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000174 DAOSME010000000
VERSION     DAOSME010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40288_length_2736_cov_20.728460"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLG98_11935"
     CDS             <1..227
                     /locus_tag="PLG98_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34020.1"
                     /translation="TQEVLIVRHGCLKVKLFTSDREYIGDRILCSGDLILLCGGGHSF
                     EMLEETAMIEVKQGPFAGDGDKTKFDEACS"
     gene            224..1381
                     /locus_tag="PLG98_11940"
     CDS             224..1381
                     /locus_tag="PLG98_11940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE34021.1"
                     /translation="MIPVSEPLIGSREIEYVNECLQTGWISSAGRFIEEFESRWAEYC
                     GVKYGVAVANGTVALQVAVECIGLSPGDKVIMPAFTIISCAQAIIRSGGIPLLVDSDP
                     RTWCMDVKQVREKVEAEMKSGDGKLKAIMPVHIYGHPVDMDPILELAEKYDLKIIEDA
                     AEVHGAEYLTGRNTPTPLWKKCGGLGHISVFSFYANKLITTGEGGMVLTNDPVYADKA
                     RSLRNLCFRPEQRFLHTDLGYNFRMTNLQAAIGLGQLERLQEIIDKKRWVGKAYTERL
                     CGIQCLQLPVEQTWAKSVYWMYGIILDENTGYTASQFAQKLREKGIETRPFFWGMHEQ
                     PVLRKMRFFTSEQYEITERIARQGLYLPSGLTITIEQIDMVCDAVQALLGQ"
     gene            1445..2329
                     /locus_tag="PLG98_11945"
     CDS             1445..2329
                     /locus_tag="PLG98_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34022.1"
                     /translation="MPFLINTANKFIKAVARRFSFESDLRQKNSPEVKINQRLLYNFY
                     RHAISTGFRCSLADTGFRVYSQFEEDGILLYIFAAIGIEHQTFIDIGSGNGINSNCAN
                     FAVNFGWHGLFIDGNQKNITKGKKYYEGNPDTWAYPPVFVNAFIQRENINELIQQNGF
                     ASKGIDLLSIDIDGNDYWIWDALNVVDPRVVIVETHIEFGLNSIVVPYDKNYNYPGKH
                     PDYHGASPVAMEKLAQRKGYRLVGANNYGFNTIYVKNGIGEDILPAVSVESIIRHPRN
                     QERQKLFDEIKDWEYSHV"
     gene            2322..>2736
                     /locus_tag="PLG98_11950"
     CDS             2322..>2736
                     /locus_tag="PLG98_11950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE34023.1"
                     /translation="MSDRIKKLSDENPQCAAEVFSDYAPYYDLLYRDKDYKAEAEYVA
                     NLIREFHPATRNILELGSGTGIHANLLAEMGFSVHGIERSQEMYTRSLDLVDKQNSQY
                     GRPTFTHGDIREIRLNKSFDAVISLFHVISYQTTNE"
BASE COUNT          758 a          627 c          658 g          693 t
ORIGIN      
        1 atacgcagga agttttaatt gtccgccatg ggtgtctcaa agtcaaactc ttcacatcgg
       61 acagggaata tatcggcgac cgcatcttgt gctcgggaga tctgattctc ctttgcggcg
      121 gcggccactc gttcgaaatg cttgaggaaa ccgctatgat cgaagtcaaa caaggcccgt
      181 ttgccggcga tggtgataaa accaaatttg atgaggcatg ttcatgatcc ccgtcagcga
      241 gcctctgatc ggttcaagag aaattgaata cgtcaacgag tgtctgcaga caggatggat
      301 ttcctccgcg ggacgcttta tcgaagaatt cgaatccaga tgggccgaat actgcggtgt
      361 gaaatacggc gtcgccgtcg ccaacggtac tgttgcgctt caggtggcgg tcgagtgtat
      421 cggactgagc ccgggggata aggtcatcat gcccgcattt accatcatct cctgcgcaca
      481 ggcaatcata cggagcggcg gcattccgct tctcgtcgat agcgatcccc gcacttggtg
      541 catggatgta aaacaagttc gtgagaaggt tgaagcggaa atgaaaagtg gggatggaaa
      601 acttaaagcc atcatgcccg tccacattta cggccatccc gttgacatgg acccgattct
      661 ggagcttgcc gaaaaatacg atttaaaaat tattgaagac gcagccgaag ttcacggcgc
      721 cgaatatctg accggccgca acacaccgac ccccctttgg aaaaaatgcg gtggcctcgg
      781 tcatatcagc gttttcagtt tctatgccaa taaactcatc actacaggcg aaggcggaat
      841 ggtcctgacc aatgatccgg tttacgccga taaagcgcgc tcgctacgca atctttgctt
      901 tcgtccggag cagcggttct tgcacaccga cctcggttac aacttccgta tgactaatct
      961 gcaggcagcc attggtctag gtcagttgga acgtttgcag gaaatcatcg ataaaaagcg
     1021 ctgggtgggc aaagcttaca cagaaaggct ctgtggcatt cagtgtcttc aattgcccgt
     1081 ggaacaaact tgggcgaaat cggtctattg gatgtatggc atcattctcg acgaaaatac
     1141 aggctatacg gccagccaat tcgctcagaa attgcgggag aaaggtattg agacgagacc
     1201 ttttttctgg ggtatgcatg aacagccggt cctcagaaaa atgagattct tcactagtga
     1261 acaatatgaa attaccgaac gcattgcccg ccagggcttg tacctgccgt caggattaac
     1321 tataacaatt gaacaaattg atatggtgtg tgacgcagtt caagctcttc ttggccagtg
     1381 aacctacttt ttaattgttc cagcacacaa acagtcctgc aacatcaatg aggatggagg
     1441 atttatgccc tttttaataa acacggcaaa taaatttatt aaggcggtgg cccgtcgatt
     1501 ttcattcgaa tcggatctgc ggcaaaaaaa cagccctgag gtaaaaatca accagcggct
     1561 cctgtacaat ttctaccggc atgcaatcag caccggcttt cgatgctcct tggctgacac
     1621 gggattccgc gtatattctc agttcgaaga agacgggatt cttttgtata tttttgcggc
     1681 tatcggtatc gagcaccaaa cgttcattga tataggctcc ggaaacggca tcaacagcaa
     1741 ttgtgcaaac tttgccgtca acttcggctg gcatggtctt tttatcgacg gcaatcaaaa
     1801 gaacattaca aaagggaaaa aatattatga gggcaatccg gacacttggg cttaccctcc
     1861 ggtatttgta aacgcattca ttcagcgtga aaacattaat gaacttattc agcaaaatgg
     1921 ttttgcatca aaaggcatag acctgctttc tattgacatt gatggaaatg attactggat
     1981 atgggacgct ctgaacgttg tggacccgcg ggtggtgatc gtcgaaacgc atattgaatt
     2041 cggtttgaac agtattgttg ttccgtatga taaaaactat aattatcctg gaaagcatcc
     2101 tgattaccat ggggcatcac ctgttgccat ggaaaaactc gcccagagaa aagggtatcg
     2161 actcgttggg gcaaataatt atggatttaa tacgatttat gtaaaaaacg gtattggtga
     2221 agatattctc cccgccgtct ccgtggaaag catcattcgt catccacgca atcaggaacg
     2281 gcagaagctg ttcgatgaaa taaaagactg ggaatatagt catgtctgat agaataaaga
     2341 agctttccga tgaaaacccc cagtgcgcgg cagaggtttt ttccgattat gctccctatt
     2401 atgatttgct ctatcgggac aaagactaca aagcggaagc cgaatatgtt gcaaatctta
     2461 ttcgcgagtt tcatccagcg acacgaaata ttcttgaact gggctctggt acgggtattc
     2521 atgcaaatct actggcagaa atggggtttt cagtacatgg aattgagcgc agtcaggaaa
     2581 tgtatacacg atccctcgac ctggttgata agcaaaattc ccaatatggc cgaccgactt
     2641 tcactcatgg agacattcgt gaaattcgcc tgaacaagtc gtttgacgcc gttatctccc
     2701 tctttcatgt catcagctac cagacaacca acgaag
//
LOCUS       DAOSME010000175         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_40345_length_2733_cov_14.133308, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000175 DAOSME010000000
VERSION     DAOSME010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40345_length_2733_cov_14.133308"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLG98_11955"
     CDS             <1..271
                     /locus_tag="PLG98_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOE34024.1"
                     /translation="GIRVPDNQICLALVRELGSPIISTSVKTEKGETLGDPAVIKEYF
                     GKTVDLIIDGGIILPQPSSVISLVDDNIDIIRKGKGDISAFLPRE"
     gene            506..2290
                     /locus_tag="PLG98_11960"
     CDS             506..2290
                     /locus_tag="PLG98_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOE34025.1"
                     /translation="MLVNAVHKEQRRMATVNEKGILVDFNIEMPTKEPITGNIYKGRV
                     LKVERGLQAAFVDYGGSKDGFLPLKDVNPEYFTEQENGQSNSKPVLKVGQEIIVQAVR
                     EAIGNKGALLTSYVSLPGRYLVLLPNKPGSGISRKVEDEEDRQKIKNLMKQIKIPETM
                     GFIVRTAGYNRTKQEIARDYQHLVRLWKEINKKAKSINKTSMIYQETDFGVRSIRDYL
                     TSEIEEILVDDLETFRKMRAYLRAISPRYIKMLTHYKEKTPLFDSYNLEEQIRVIYQE
                     RVELKSGGHIVINPTEAMITIDVNSGRGSNKKNIEETAFKTNIEAGEEIARQLRLRDL
                     GGLIAIDFIDMMDKKHIAEVEKSFKKALSIDKARIQLSRISKFGILELSRQKKQSTIQ
                     EISYTKCPYCKGSGLRPSLEYAALGAYRKVESEAVKGMCSELKITLPHEIADYLLNRK
                     RSELLDLESSFKVALNICGSSEIPWDRVEIQQIVKEPTDNHEHLPEAVEAKGEETPTT
                     ADDEKAAPGGEKPVRKRGRRRPRHKRRKPAGSTAAIASPETASDDAAGEGLSSLMIIQ
                     PAENKEDGVPGGKETPESHIAATTTDNE"
     gene            complement(2302..>2733)
                     /locus_tag="PLG98_11965"
     CDS             complement(2302..>2733)
                     /locus_tag="PLG98_11965"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOE34026.1"
                     /translation="EGTAAVAAGRHAKANDVRITRAIFNAVGISVEVREQLMDAVTGL
                     SGSGPAYFFLMIEALIEAGVKTGLSKDLAKKLATQTMLGAARLCIESDKEPAELRAMV
                     TSPNGTTFAGLKVMEEKNVRGIMAAAVKAATRRSKELAKGK"
BASE COUNT          766 a          681 c          703 g          583 t
ORIGIN      
        1 gggtataagg gtacccgaca atcagatttg tctggcgctg gtccgggaac tcggatcgcc
       61 cattatcagc accagcgtga aaacggaaaa gggcgaaacg ctgggcgacc cggccgtgat
      121 caaggagtat ttcggtaaga cggttgattt aattattgac ggcggcatta ttctgccgca
      181 gccgtcaagc gttatcagtc tggttgacga caatatagat attataagaa aaggaaaagg
      241 cgacataagc gcttttctgc cacgggaatg aactctttag tattttttgt gattcattcc
      301 ctccgctacg cgggattgtt caccttacca attccgatac gcgtcgcctg gcaacaccag
      361 gcttcgcttt cgtaattgga ggtttactga taaatataaa aggctgagcg gtatttttaa
      421 ccgaaagacg catcaaaggt ctgccggagc gcgggtgcgc gcggaaaaat atcagccgcc
      481 gaaaaggaca tctatgaaac atattatgct cgttaacgct gttcacaaag aacagagacg
      541 aatggctacg gttaacgaaa agggcattct ggttgatttt aacatcgaaa tgcctaccaa
      601 agaacccatc accggaaata tttacaaagg cagggttctg aaagttgaac gcggcctgca
      661 ggcggccttc gttgattacg gcggcagcaa agacggtttt cttccgctta aggacgtcaa
      721 tcccgaatac ttcactgaac aggaaaacgg tcaatccaac agcaagccgg ttttaaaagt
      781 cggacaggaa attattgttc aggccgtccg ggaagccatc ggcaacaagg gagccctgct
      841 cacctcctat gtttccctgc ccggccgata tctggtcctg cttcccaaca agcccggcag
      901 cggcatttcc cgcaaagtgg aagatgaaga agaccgccag aaaataaaaa atctcatgaa
      961 gcagattaaa attccggaga cgatgggatt tatcgtccgg acggcgggct ataaccggac
     1021 aaagcaggaa atcgcccgcg attaccagca cctggtgcgc ctctggaaag aaatcaataa
     1081 aaaggcaaaa agcatcaaca aaacgtccat gatttaccag gagacagatt tcggcgtgcg
     1141 cagtatccgc gactatctga cgtcggaaat tgaagaaatt ctcgtcgacg atctggaaac
     1201 gttccgcaaa atgcgcgctt atttgagggc gatatctccc cgttacatca aaatgctgac
     1261 gcattacaag gagaagaccc cgcttttcga cagttataat cttgaagagc agattcgcgt
     1321 catttatcag gaacgcgtgg aactgaaatc cggcggacac attgttatca atccgacgga
     1381 agcaatgata acgattgatg tcaattccgg acgcggctcc aataaaaaaa atatagaaga
     1441 gacggctttt aaaaccaaca tcgaggcggg ggaggagatc gcccgtcaat tgagactcag
     1501 agacctgggt ggattgattg ccatcgattt tatcgacatg atggataaaa aacacatcgc
     1561 cgaggtggaa aaatccttta aaaaagcgtt gagcatcgac aaagccagga tccagctttc
     1621 ccgaatttcc aagttcggca ttctggaatt atcccggcag aagaagcaat ccaccattca
     1681 ggaaataagt tacacaaaat gtccttactg caaaggcagc ggcctgcgtc cgtctctgga
     1741 atatgcggcg ctgggcgcgt accggaaagt cgaatccgag gccgtgaagg gaatgtgttc
     1801 cgaattgaag ataacgctgc cgcatgaaat cgccgattat cttctcaatc gcaagagaag
     1861 cgaattgctg gatctggaat cgtcatttaa ggtggcgtta aatatctgcg gaagttctga
     1921 aataccctgg gaccgggtgg aaattcaaca gatcgtaaaa gaacccacag acaaccacga
     1981 gcatctgccg gaggctgtcg aggcaaaagg ggaggaaacg ccgaccaccg ctgacgatga
     2041 aaaagctgcg cccggcggag agaagcccgt caggaaaaga ggacggcgca gaccgcgaca
     2101 taagagaaga aaaccggcgg gatcaaccgc agccattgct tcgccggaaa ccgcctccga
     2161 tgacgccgcc ggagaagggt tatcaagcct gatgatcata cagccggcgg aaaacaaaga
     2221 agatggtgtt cccggaggga aagaaactcc ggaaagtcat attgccgcaa ccacaacgga
     2281 taacgaataa caggcaggaa gttattttcc tttcgccagc tctttggagc gtctggttgc
     2341 cgctttcacg gcggcagcca ttatcccgcg aacgtttttt tcttccataa cttttaatcc
     2401 ggcgaaggtc gtgccgttgg gcgatgtcac catggccctg agctccgccg gttctttatc
     2461 gctttcaatg cacagacgcg cagctcccag catggtttgc gtcgccagtt ttttcgccag
     2521 atctttcgac aatccggttt taaccccggc ctcaatcagc gcttcgatca tcaggaaaaa
     2581 ataagcgggg ccgctgccgc tcaatccggt caccgcgtcc atgagctgtt cccgcacctc
     2641 cacactgatg cccacggcgt tgaagatcgc gcgtgtgatc ctgacgtcgt tagctttggc
     2701 atggcgtccc gcagccaccg ctgcggtgcc ttc
//
LOCUS       DAOSME010000176         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_41460_length_2678_cov_29.347693, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000176 DAOSME010000000
VERSION     DAOSME010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41460_length_2678_cov_29.347693"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLG98_11970"
     CDS             complement(<1..1185)
                     /locus_tag="PLG98_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOE34027.1"
                     /translation="MPSTPNIPPSAAPRLPKEEIDLFGYIDVILRRWKICALAFCVVF
                     VLVALYTFFMKPVYESFALLHVKDNKSKAGLLSELSFSSTNPINTEIEILKSRTNAEN
                     VTRQLHLNWRVSKKSEGLTFKILEFSSDAKQPVYKIRLTGSDTFDVYNNGDLVGRGKS
                     GSLLRKDGFSLLLTDLRGSKGDDFQLALSKFEDVVASLQAGIKAAEQGRMTGIIRVSY
                     ASTNPVLARDIVNTLVQAYLEQSISFKSEEASRAVGFVEEQLKNLRGDLDTSEKNLQG
                     FKSTTGVIKLDSEAEQLITKLADLEKSRAEVVLQQNQIKFALDALENAVKTGGAYAPV
                     SINSDPILSGMAVRLNELEVQKRSLQTDYTESHPAVKTLQGQIEAMQKKMLAGYKTSL
                     SNL"
     gene            complement(1251..2393)
                     /locus_tag="PLG98_11975"
     CDS             complement(1251..2393)
                     /locus_tag="PLG98_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOE34028.1"
                     /translation="MRANSKYFYFLIVLLFAAATLLPAGCARHRDMVSGTVKDITEPT
                     PGAPLKQPEAKMIYSANAKSPVVELENVTIPPLKEPPPPPDYIVGPNDTIMVTVAGHP
                     EFSPGMFPSTTTGGATGTQRGSQVDGSGYVQIPTLGLVRVAGLTLPQVRSHMQELLKQ
                     YLREPSVVVEVVQYRSQPLYILGQFKNTGVFYMDRPFNVLQGLAFGGGYDLATANPKA
                     ARIIRNNRVLPVDVYELLMRADQSQNVWLKPNDTIFMPDNKNQMVFVFGAGKAGMTIP
                     LPASGMNLLQAIAAAGFQEIGYHSRRVFLIRSLSATRGQLMVVDVDMMVRGDAIPLEL
                     CEGDVIYMPKSAMTSWNEAVGEILPTLQAVGAIISPFVQLKYLFDK"
BASE COUNT          588 a          640 c          766 g          684 t
ORIGIN      
        1 cagattagac aggcttgttt tgtagccggc cagcatcttt ttttgcatcg cttcgatctg
       61 tccctgaagg gtctttacgg ccggatgcga ttccgtgtaa tcggtttgaa gcgagcgctt
      121 ctggacttcc agttcgttta gcctgacggc cattcctgac agaatcggat cgctgttgat
      181 cgaaacgggg gcataggcgc cgccggtctt tacggcgttt tccaaagcat ccagtgcaaa
      241 ctttatctgg ttttgctgaa ggacaacttc tgcccggctt ttttcaaggt cagcaagttt
      301 tgtgatcagt tgttcggctt cgctgtccag tttgatcacg ccggtggtac ttttaaagcc
      361 ctgaaggttt ttctccgatg tgtccaaatc gccccgcagg tttttaagct gctcttccac
      421 aaagccgacg gcgcggctgg cttcttcgga cttgaaggaa atggactgct ccaggtaagc
      481 ctgcacaagc gtgttgacga tgtccctggc aaggacggga ttggtgctgg cgtaggacac
      541 ccggatgatg ccggtcatcc tgccctgttc cgccgcctta atgccggcct gaagactggc
      601 aacaacatct tcgaatttgg aaagagccag ttgaaagtcg tcgcccttgc tgccgcgaag
      661 atcggtcaaa aggaggctga aaccgtcttt ccgcagcagt gagccggact ttccccggcc
      721 gaccagatcg ccgttgttgt aaacgtcaaa cgtatcgctg ccggtcagtc gaattttgta
      781 gaccggttgt ttcgcgtcgg atgaaaactc caggattttg aaggtcagac cttctgattt
      841 ttttgaaacc cgccagttaa gatgaagctg cctggttacg ttctctgcat tggtgcgcga
      901 tttcagaatt tcaatctctg tgttgatggg attggtggag ctgaacgaca gttcgctgag
      961 caggcctgct ttacttttgt tgtccttgac gtgaagaaga gcaaaggatt cataaaccgg
     1021 tttcatgaag aacgtgtaga gggcgacgag gacaaaaaca acgcaaaagg ccagggcgca
     1081 aattttccat cgcctcagga tgacgtcgat gtagccgaac agatcgattt cttcttttgg
     1141 aagacgcggt gccgcagacg gcggaatgtt gggagttgag ggcataatat cctttaaagt
     1201 attaagtatt aagggttaag taaaaacagg ctatgggcca tggttttgcc ttatttgtcg
     1261 aacaggtact ttagctggac gaagggcgaa atgatggcgc cgacggcctg cagtgtgggc
     1321 aggatttcgc ccacggcttc gttccaactg gtcatggcgc tcttgggcat gtaaatgacg
     1381 tcgccttcgc acaattccag gggaattgca tcgccccgca ccatcatgtc cacatcgacg
     1441 accatcaact gcccccgcgt tgcggaaagg gagcggatca gaaaaacgcg ccgtgaatga
     1501 tagccgattt cctgaaagcc ggccgcggca atggcctgca gaagattcat ccccgaagca
     1561 ggcagcggta tggtcattcc ggctttgccc gcgccgaaaa caaacaccat ctggtttttg
     1621 ttgtccggca tgaagatggt atcgttgggt ttgagccaaa cattctggct ctgatcggcc
     1681 ctcatcagca attcgtaaac atcaacggga agaacgcggt tgttgcggat gattctggcg
     1741 gctttgggat tggctgtggc cagatcgtac ccgccgccaa aggccagccc ctgcaggaca
     1801 ttgaaggggc gatccatgta gaaaacgccc gtgtttttaa actggcccaa aatgtacaac
     1861 ggttggcttc tatattgaac cacttccacc acgacagaag gctcgcgcag gtactgcttc
     1921 agcagctcct gcatgtggct gcgtacctgg gggagcgtca ggcctgccac gcgcaccagt
     1981 cccagcgtgg gaatctgaac gtagccgctg ccgtccacct ggctgccgcg ctgtgtgccg
     2041 gttgcaccac ctgtggttgt cgatggaaac atccccgggg aaaactcagg atgcccggca
     2101 accgtaacca ttatcgtatc gttgggcccg acaatgtaat cggggggagg aggcggttcc
     2161 ttgagcggcg gtatggtgac gttttccaat tccacaacgg ggcttttggc gtttgcgctg
     2221 tagatcatct ttgcttccgg ttgttttaag ggagcgcccg gtgttggttc cgttatgtcc
     2281 ttgacggtgc ccgacaccat gtcccgatgc ctggcgcagc ctgccggcag cagcgtggcc
     2341 gcggcgaaaa gaagaacgat caaaaaatag aagtattttg aatttgcgcg catcagatta
     2401 tgccccatga atgttcttgt ttaatgattt taggttcata gcaaataacg gaaggagtga
     2461 caaggaattt agaagtttag agggttagag ggttagaggg ttagaggttt ggaggtttgg
     2521 aggattagag gattagaggc ttggaggttt ggaggcttgg aggttttaga tttcattgtt
     2581 gcctcattac tgtttcctct ctggcatttt aatcaaagtt ttaatacagc tccgccctct
     2641 gggttacagg aaacgggtta ggggtacgag gttatggg
//
LOCUS       DAOSME010000177         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_41824_length_2660_cov_19.578887, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000177 DAOSME010000000
VERSION     DAOSME010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41824_length_2660_cov_19.578887"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLG98_11980"
     CDS             complement(<1..559)
                     /locus_tag="PLG98_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE34029.1"
                     /translation="MNDEILKYWIALKSIKGIGHVSFPALVDRFGSPAAVFGASVSDL
                     QQTEGISRQIAEAIVHFRGWDNVKAELELIRKNDVTIVTYEDELYPGQLLNIYDRPPY
                     IYVRGNLSREDINLAIVGSRAASTYGKYTTERISRDLALQGVTVVSGMARGIDSSAHR
                     GAITAHGRTVAILGSGLDVIYPPENK"
     gene            767..2500
                     /locus_tag="PLG98_11985"
     CDS             767..2500
                     /locus_tag="PLG98_11985"
                     /inference="COORDINATES: protein motif:HMM:NF040132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34030.1"
                     /translation="MFKVKNILISFITIFFIYLPAGFAQNVTKTQKPSVAPKQGVQKS
                     VDQATKKPKPGLTIMPAGINLGLVAFDKSVEATVSLKNTGTGAIRWSIQGPKSWDKVK
                     SKAISGTLEKETDVLNVGVSIQGSDESKDEKKAGHSLANVEMRIASGEEEIICTKKLP
                     LGIHKEEIEINAADGPKKITLSFFIAYTQSAPRINLSTLRLDMGKILPNKTVSRKIIL
                     NNSGKETLTWSVEPPAHAREDMPDDFRQGRYLSFMNDEVKGAAQYSVPEPLKDTLELI
                     GSWSETGGYPTSGPGDNLIKVNFTGTGILLYLSEHSREGKLTLALDKNPIARAALFEE
                     QRKNGEEILIADQLEFGSHTLTITNKYEGLVVEGVKILGLNTAFFPAQSIKIFPASGA
                     TTLQTNYLTVLFNTGQAFPGFYEDYLVFKTNNGEAIVEMYAEVLPDVTSAIIDVYRYY
                     NGNDYLFTSNPQAEINRLYQNKYTKEGIAFRLFQPGTPGTASFYRWYHPRKKCHFYHY
                     DYNGHKQDLHGYILEGTLGNIATSKLTNTRELYRWYNAKTGHYFYSTELQGEKINKKA
                     YRFDGIAGYVR"
BASE COUNT          789 a          618 c          635 g          618 t
ORIGIN      
        1 ttttattttc cggaggataa atgacatcca ggccgctgcc cagaatcgca acggttctgc
       61 catgagcggt tatagcgccg cgatgcgccg acgaatcgat gccccgagcc atcccgctga
      121 ccaccgtcac gccctgaagg gccaggtccc ggctgattct ttccgtcgtg tatttaccgt
      181 aagtgctcgc tgcacgggaa ccgacaatgg caagattgat gtcttctctg ctcaaatttc
      241 cccgcacata tatataagga gggcggtcat agatattcaa taattgcccc ggatagagct
      301 catcttcgta agtaactatt gtaacgtcat ttttgcgaat aagttcaagt tctgctttaa
      361 cattatccca gcctctgaaa tgaacaatgg cctctgcaat ctgacgggaa attccctctg
      421 tttgctgcag gtcggaaacg gaagcaccga aaacagcggc aggagagccg aacctgtcca
      481 ccagtgccgg aaaagagacg tggccgatgc ctttgattga ttttaacgca atccagtact
      541 ttagaatctc atcattcata aatgacccga acgagaagcg ttatggtgat gattggggat
      601 tttgaaaaat tgacagcaac ctacatcttg tctgccggat gtgtcaagta ttttcaaaat
      661 gatgatccgc cggatgaaca aagagtgaaa aatgtgaaat tcttaaaaca gttgcttaat
      721 tttaagcttt gagctaggaa accatcaacg tttcatagag gtcgtcatgt tcaaggtaaa
      781 aaatattctg atttctttta tcacaatttt ctttatttac ctgcccgccg gttttgctca
      841 aaacgtcaca aaaacgcaga aacctagcgt tgcgcccaaa cagggtgttc aaaagtcagt
      901 tgaccaggca acgaaaaaac caaaaccggg cctgaccatt atgccggcgg gaattaattt
      961 gggtcttgtc gcctttgaca aatcggtgga agcaaccgtc agtttaaaaa atacgggtac
     1021 cggcgcgatc cgctggtcca tccaggggcc taagagctgg gacaaagtaa aatccaaagc
     1081 gatatcggga acactggaga aggaaactga tgttttaaat gtaggcgtga gtatccaggg
     1141 cagcgatgaa tccaaagacg aaaaaaaagc cgggcattcg ctggcgaatg tcgaaatgag
     1201 aattgcctcc ggagaggaag aaattatctg taccaagaaa cttccgctag gaatacataa
     1261 agaggaaatt gaaatcaacg ctgcggacgg gccgaaaaaa ataaccttgt cttttttcat
     1321 tgcctacacg cagagcgctc cccggatcaa cctcagtacg ctgagattgg atatgggaaa
     1381 aatcctgccg aataaaacag tttccagaaa aataatatta aacaacagcg gtaaggaaac
     1441 gttgacgtgg tcggtcgaac cgccggcaca cgcccgggaa gacatgccgg atgatttcag
     1501 gcagggaagg tacctgtcct tcatgaatga tgaggtcaaa ggcgccgcac aatacagcgt
     1561 tcccgagcct ttgaaagaca cactcgagtt aatcggaagc tggtcggaaa ccggcggcta
     1621 tccaaccagc gggccgggcg ataatctgat caaagtcaat tttaccggaa cggggatcct
     1681 tttatacctg tccgaacatt cccgggaagg aaaactgacc ctggcgctgg ataaaaatcc
     1741 aatcgcgagg gccgccctgt ttgaagagca gagaaagaac ggggaagaaa tcctgatagc
     1801 cgatcagctg gaatttggtt cccataccct cacgatcacg aacaaatatg aaggtctggt
     1861 cgtggaaggc gtaaaaatac tgggactgaa cacagccttt ttccccgctc agagcataaa
     1921 aatctttcct gcgtccgggg ccaccaccct gcagaccaat tatctgaccg ttttatttaa
     1981 taccgggcag gctttcccgg gcttttatga agactacctg gtctttaaaa ctaataatgg
     2041 cgaggcgatt gtcgagatgt atgcggaagt gctgccggat gtcacctcgg cgatcatcga
     2101 tgtttacaga tattacaacg gaaacgacta tcttttcaca tccaatccgc aggcggaaat
     2161 caacagattg tatcagaaca agtacacaaa agaagggatc gctttccgtt tattccagcc
     2221 cggcacgccg ggaaccgcga gcttctaccg ctggtatcat ccccggaaaa aatgtcattt
     2281 ttatcattat gactacaacg gccataaaca agatttgcac ggttatattc ttgaagggac
     2341 gctcggcaac atcgccacct cgaaactgac caacacccgg gagctttacc ggtggtataa
     2401 tgcaaagacg ggccattatt tttattcaac ggagttgcag ggagaaaaaa ttaacaaaaa
     2461 agcatatcgt tttgacggca tagccggtta cgtccggtaa ccatcggaat gtttcggaaa
     2521 aataatgcct caagggccgt atttcatctt gacaaaaaaa agaagagacg atattaggct
     2581 aagtcttttt aggcacaggt aaaacatgcg cctgtagctc agttggatag agcaacggac
     2641 ttctaatccg tgggccgaga
//
LOCUS       DAOSME010000178         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_41875_length_2658_cov_16.906262, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000178 DAOSME010000000
VERSION     DAOSME010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41875_length_2658_cov_16.906262"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..500
                     /locus_tag="PLG98_11990"
     CDS             81..500
                     /locus_tag="PLG98_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34031.1"
                     /translation="MKKLQMVMAVIFVLCAGAAVSAQDVKPAYDMTGIWRSQDPGTAQ
                     VFQNGLEIRSIYINRGFSHFLTGTYINPTTIKGVQYRRNRSNGCLTVMAITMNVQSPD
                     ASTGDWEAMDGNCDLRQGQKGSWRSQRDRQLEQSTWY"
     gene            888..2069
                     /locus_tag="PLG98_11995"
     CDS             888..2069
                     /locus_tag="PLG98_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE34032.1"
                     /translation="MTADPKTMSEGDRSTYIHFIDWLKQSWYGVPDADVVFPYIAARY
                     TPEEALLLTGFPYAPTALADLAALKKMSSDALRPILDSLTAKGLLNKQRKGDVFRYNL
                     HDIFTIYRGFGWPGKTDDYSKTVGPLQHRYIMEGLMSPWRNAKEKGLRVLPIGVTIQD
                     QRGVVPYEAVGRILDQVEYFTVSHCPCRHANNLNPDAPDSPFPTDVCLHFDKLGHYIV
                     ENGMGREITRQETEAILKQCADLGLIHGISNYQEKPDTICNCSSDCCIWFMAMKKYGH
                     DGTLTPSNFRVSVNRSACTGCGLCVKRCLMEALHLEEDLAAKGRETLVPGKDGKQRTL
                     INQTGKISQLAPERCIGCGVCAYKCPSGAITLERNPVEHAPPQTGRDWIMQFLTDTRT
                     A"
     gene            2151..2369
                     /locus_tag="PLG98_12000"
     CDS             2151..2369
                     /locus_tag="PLG98_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOE34033.1"
                     /translation="MPKVLHIRLTDDLSSRLDFLAEKTKRPKSFFIKEILGSYLPELE
                     DTYLALDRHNDRNAKYYSTEDMEKILDL"
BASE COUNT          689 a          669 c          668 g          632 t
ORIGIN      
        1 tgggggttca tgtccatttt ttcaactaga aaaagtacgc atcaaccgga tgaacttgat
       61 tttacaaggg aggggattgc atgaaaaaat tacagatggt gatggccgtt atttttgtgt
      121 tgtgcgcggg agccgcggtt tctgcccagg atgtcaaacc ggcttacgat atgaccggaa
      181 tctggcgttc acaggatccg ggcaccgccc aggtattcca gaacgggctt gagatccgat
      241 cgatctatat caacagggga ttcagccact ttcttaccgg aacctacatc aacccgacaa
      301 cgatcaaagg cgtgcagtac cggcgcaacc gctccaacgg atgcctcacc gtcatggcca
      361 taacgatgaa tgttcagtcg ccggatgcct cgacgggcga ctgggaggcg atggacggca
      421 actgcgactt gcgccaggga caaaaaggct cctggcgatc gcaacgggac aggcaattag
      481 aacaaagcac gtggtattga ttcggctggt ttgagagcaa aaaggcgggc cttgtcaggt
      541 tttcggccgg gttggaaaaa cgtttgttac gttttttccc cgccgtgttc ctgaaaaggc
      601 ccgcctggtt tcaagatgga tattcttttt cgttgcgatc gctcacgttt ggaaaggctt
      661 ccttgtagat accttcatgc tgattacttc gagagaatct atccccggta atgtccccga
      721 ggcaatcctt aacattgaag acagatctgt ctggccttat caagcatttc cggcgtcata
      781 tcaactgaag acattcatcg cttgctgatc cgtgaattcc gtgctagtca gcacgcgtat
      841 ctaaaataac atcgatgaac gctttttgtt ctcctgaagg gttttgtatg actgccgatc
      901 caaagacaat gtcagagggg gaccgatcca cctacataca ttttatcgac tggctcaaac
      961 agagctggta cggcgtgccg gatgcggatg ttgtgttccc gtatatcgcg gcgcgctaca
     1021 cgcctgaaga ggcgcttttg ctgaccgggt ttccatacgc tcccacggcg cttgcggacc
     1081 ttgcggcgct caaaaagatg tcatccgatg cacttcgccc gatcctcgat tcgcttaccg
     1141 caaaagggct cttaaacaaa cagagaaaag gcgatgtctt ccgctacaac ctgcacgata
     1201 ttttcaccat ctaccgggga ttcggctggc cgggaaaaac ggacgattac agtaaaacgg
     1261 tcgggccgct gcagcacagg tatatcatgg aagggctgat gtcgccgtgg cggaatgcaa
     1321 aagagaaggg actgcgggta ttgcccatcg gggtcaccat tcaggaccag cgcggtgtgg
     1381 tgccctatga ggcagtcggc cggattctcg atcaggttga atatttcacg gtcagccact
     1441 gtccgtgtcg tcatgccaac aacctgaacc cggacgcgcc ggattctcca tttcccaccg
     1501 acgtctgcct gcatttcgac aaactcggcc actatattgt cgaaaacggg atgggccggg
     1561 agatcacccg acaggaaacg gaagcgatcc tcaagcaatg cgccgacctg ggactcatcc
     1621 atggaatctc caattaccag gaaaaacccg atacgatctg caactgctct tcggattgct
     1681 gcatatggtt catggccatg aaaaaatatg gtcatgatgg aacgctgacg ccgtcgaact
     1741 ttcgcgtttc cgtcaatagg tccgcttgca ccggctgcgg actgtgcgtc aagcggtgtt
     1801 tgatggaagc tcttcattta gaggaggatt tggccgccaa aggacgggaa accctggttc
     1861 cgggcaaaga cggcaaacag cgcaccttga tcaatcagac cggaaaaatc agtcaactcg
     1921 cgccggagcg ctgcatcgga tgcggtgtat gcgcttataa gtgtccgtca ggagcgatca
     1981 cgctggagcg caatccggtg gagcatgccc cgcctcaaac cggtcgcgac tggatcatgc
     2041 aattcctgac ggatacccga acagcctagg gcgcctgaag ggactgaaaa ggttttccac
     2101 tctgacgtgc cataggcata tccggctttt cacaatagag aggttaaacc atgcccaaag
     2161 tattacacat ccgtctaact gatgacctat catctcggct tgatttttta gccgaaaaaa
     2221 caaaaagacc aaaaagtttc ttcataaaag aaattcttgg ctcgtatctt cctgagctcg
     2281 aagacactta tcttgccctt gatcggcata atgacagaaa tgccaaatat tactcaacag
     2341 aagacatgga gaagattctt gatttataat atccgttggc atgaagaagc aataaatgat
     2401 ctcaaaagaa ttgaccggca aacataaaag aagatcatcg ccaggataaa agattatctt
     2461 tcaaaggatc ctttgagtct tggtaagcca ctccaggggg gaatctttaa gggtctttat
     2521 cgttatcgct acggatcata ccgcataatt tacgccatag acagagattc cgtaattatt
     2581 ctgatattga gaatagctcc acatacaacg tcttcttccg atacttcagt atccatacca
     2641 cgtgatactt gcatttcc
//
LOCUS       DAOSME010000179         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_43592_length_2579_cov_20.152139, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000179 DAOSME010000000
VERSION     DAOSME010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43592_length_2579_cov_20.152139"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLG98_12005"
     CDS             <1..687
                     /locus_tag="PLG98_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34034.1"
                     /translation="DYVNVLINCRKAIYVEFETDYDISLYGDEREKIMGTMAIMLTGM
                     KAPFYVCNKKYIDEYVKEPTDYIVYDHDRLDMDLLRAGISRVDYWNVWRLTPSLYRKN
                     KESDWIVKKDFQKLDEEGIKDRAEYVLDSSINLILTKHLDIKRSKSPDYKKYYVSLKN
                     ESVPIYEKADNKSKVVGTVPANCKKVFVDSKVSALNGKGIFWHVVHVSVEDNFYLWGY
                     IDNDDIINSN"
     gene            complement(822..1985)
                     /gene="rpsA"
                     /locus_tag="PLG98_12010"
     CDS             complement(822..1985)
                     /gene="rpsA"
                     /locus_tag="PLG98_12010"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOE34035.1"
                     /translation="MTEEKSFSEMLDETSLKPRRFSAGEKIDTIIVKITEEWMFLDLG
                     AKSEGYLDKKELLDENGNLTVKEGDPITAYFVSSRHGEKLFTTKLLTSKSVDDFMFKA
                     YKNQIPVEATVEKEIKGGFSVRINQNVSGFCPYSQMDVRRIDDVTAYVGKKFEFIVAE
                     YSEKGRNIILSRRPLLEKIEQEKKAALKNSLQQGMTVSGVVTSVQKFGAFVDLGGVQA
                     LMPVSEMGWGRVEDPKVLYSPGDKIEAVIINLDWEANRITLSAKASLPNPWDGFVRKY
                     TEGSLLKGKVTNLTNFGAFVSLEDGVEGLLHISKLARGKKIKHAGDVMKTGEELEVKI
                     EKIDRENRKISLDLTGSDKDTSAASGNEDDFRGYIGKSPKTMGTLGDLIKKKK"
     gene            2110..>2579
                     /locus_tag="PLG98_12015"
     CDS             2110..>2579
                     /locus_tag="PLG98_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOE34036.1"
                     /translation="MRFEISRGGSGLTYEIRNIVLIANKLKEYGVDIIWENIGDPVQK
                     GEKIPDWMKEVLFEIIKDDESFAYSPTKGVNATREFLTERLNARGNVRITPEDIIFFN
                     GLGDAIARAYSSIQVDARMIMPEPTYSTHFMAEVLHASFPPNTYRLNPYNGWHP"
BASE COUNT          731 a          563 c          526 g          759 t
ORIGIN      
        1 gattatgtta atgtccttat caattgcagg aaagccattt atgtagaatt tgaaacggac
       61 tatgacataa gtctatatgg agatgaaaga gaaaaaataa tgggtactat ggctattatg
      121 ctaacgggaa tgaaagctcc attttatgtt tgcaataaaa aatatatcga tgaatatgtt
      181 aaagaaccga cagattatat tgtctatgat catgaccgtc tagatatgga tctgttaagg
      241 gcaggaatta gccgggtcga ttattggaat gtttggcggc ttactccatc tttatatcga
      301 aaaaacaaag aatcagattg gattgtaaag aaagattttc aaaaacttga tgaggaaggt
      361 ataaaagata gagctgaata tgttctcgac tcttcaatta atttaatttt aacaaaacac
      421 ttagatataa aacgttcaaa atcaccagat tataaaaagt attatgtgag tttaaaaaat
      481 gaatcagtcc caatatatga aaaagctgat aataaaagca aagttgtcgg aactgtgcca
      541 gccaactgca aaaaagtatt tgttgactca aaagtttcag ctctaaatgg aaaaggtatt
      601 ttttggcatg ttgttcacgt atcagtagaa gataactttt atctttgggg atatattgac
      661 aatgatgata ttataaatag taactgacaa cggcattaat ggcaacaaca aaaagccgcc
      721 acacctcata tcaggcgttg cacctttcaa ctagtaatcc tcaattttct ttattctata
      781 aaaatgtgga cttgtaggta ttttcttttt caacccagaa tttatttttt cttcttgatc
      841 aggtcgccca gcgtgcccat cgtcttgggt gatttgccga tataaccgcg gaaatcatct
      901 tcatttcctg aagcagctga agtatctttg tcgcttccgg tcagatcaag agaaattttt
      961 ctgttctcgc ggtcgatttt ttcaatcttc acttccaatt cttcgccggt tttcataaca
     1021 tcgccggcgt gtttgatttt tttgccgcgc gccagtttgg aaatatgcaa aagcccttcc
     1081 accccgtctt ccagcgaaac aaaagcgccg aaattggtca ggttggttac cttgcccttg
     1141 agcaggctac cttcggtgta ttttctcaca aagccgtccc acggattggg aagagaggct
     1201 ttagccgata acgtgatgcg attagcttcc cagtccagat tgatgatcac ggcttcaatc
     1261 ttgtcgcccg gcgagtagag tactttggga tcttcaaccc gtccccagcc catttccgaa
     1321 acaggcatga gggcctgcac accgccgaga tcaacgaaag caccgaattt ctgaacgctt
     1381 gtcactacgc cgctcaccgt catcccctgt tgcagggaat ttttcaacgc ggcttttttc
     1441 tcctgttcga ttttttccag caaaggccga cgtgacaaaa tgatattgcg acccttttcc
     1501 gaatattcgg ccacaataaa ttcaaatttc ttgccgacgt aggcagtgac atcatctatt
     1561 cttctgacat ccatctgcga gtagggacag aacccgctga cattctggtt aatcctgacc
     1621 gaaaagccgc ctttgatttc cttctcgaca gtcgcttcaa cgggaatctg atttttatag
     1681 gctttgaaca taaaatcatc aacactcttg ctcgtcagca gtttggtggt aaaaagtttt
     1741 tccccgtgac gagaagagac aaaataagcc gtgatgggat ctccttcctt cacggtgaga
     1801 ttgccgtttt catccaatag ttccttttta tccagatagc cttcactttt cgctcccaga
     1861 tccaggaaca tccattcctc tgtaattttt acgatgatag tatctatttt ctctccggcg
     1921 gaaaaacgcc ggggttttaa gctggtttca tcaagcattt cagaaaaact tttttcttcc
     1981 gtcatattgt cctctttatt tttacgcccg acaggcgttt cgtttggtgc tccgatagca
     2041 taaaaaactg gaaatttaaa caaatatgta ttatgaaata caaatatcat ctaaggggtt
     2101 aaaatattta tgcgttttga aatttcccgc ggcggcagcg ggctgactta tgaaatacgg
     2161 aatatcgtac tgattgccaa caaactgaag gaatacggcg ttgatatcat ctgggaaaat
     2221 atcggcgatc cggtgcaaaa gggagaaaaa attcccgact ggatgaagga agtcctcttt
     2281 gaaatcatca aagatgatga gtcttttgcc tattccccga cgaaaggtgt taacgccacc
     2341 cgggagtttc tcaccgagcg tcttaatgcg cgcggaaatg tgcggattac ccccgaagat
     2401 atcattttct tcaacggtct gggcgatgcc attgcccgcg cctacagctc cattcaggtt
     2461 gacgcccgca tgatcatgcc ggagccgacc tattccacgc acttcatggc ggaagtgctg
     2521 cacgcgtcgt ttccgcccaa tacctaccgg ctgaatccat acaacggatg gcatccgga
//
LOCUS       DAOSME010000180         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_44408_length_2544_cov_19.634793, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000180 DAOSME010000000
VERSION     DAOSME010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44408_length_2544_cov_19.634793"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLG98_12020"
     CDS             complement(<1..341)
                     /locus_tag="PLG98_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOE34037.1"
                     /translation="MADMDRSKAVDLAITQIEKQFGKGSIMKLGGSEKVDVPAISTGS
                     LSLDIALGTFGVPRGRVVEIYGPESGGKTTLALHIVAEAQKKNGIAAYIDAEHALDVG
                     YAQKIGVNTDDL"
     gene            complement(545..1372)
                     /locus_tag="PLG98_12025"
     CDS             complement(545..1372)
                     /locus_tag="PLG98_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HOE34038.1"
                     /translation="MNDLQKMEHNQIVVFGSKKIRRIWHDEEWYFSVIDICGALTNSP
                     DAGAYWRKLKQRLNVEGSEVVTFCHGLKLEAADGKKYATDCANTESMFRIIQSIPSPK
                     AEPFKRWLARVGYERVQEIENPELAQERMKLLYEQKGYPRDWIDKRLRGIAIRQNLTD
                     EWRERGIISERDYAILTAEISKATFGITPADYKKLKGLFKKNQNLRDYMTDLELIFTM
                     LGEKVTTEISQKEKPDTFEKNRKVAKRGGGVAGTARKETEKELGRSIITNQNYLKEK"
     gene            complement(1531..2118)
                     /gene="thpR"
                     /locus_tag="PLG98_12030"
     CDS             complement(1531..2118)
                     /gene="thpR"
                     /locus_tag="PLG98_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOE34039.1"
                     /translation="MTADNEKNIRAFLAIEPPENILNEISRLQEKLKREINGRLSWTR
                     PQGQHLTLKFFGDISKEDINSICATVQKRVVAEQKLNLKVEKMGVFPDARRPRVLWCG
                     VTGDVEKLITLQKKLDADFATLGFLEEDRSFKAHLTLARIKDPRDITGMSEALKKYDS
                     FKAGEFVADKLFLFQSKLSPQSAVYTKLAEFALGG"
     gene            complement(2350..>2544)
                     /locus_tag="PLG98_12035"
     CDS             complement(2350..>2544)
                     /locus_tag="PLG98_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOE34040.1"
                     /translation="IAGPTGGTKEKPVGTVYLAVADAQKTICRHYTFRWDRKRNKEVF
                     SEAALFLLKNFCETQIAKTK"
BASE COUNT          597 a          643 c          508 g          796 t
ORIGIN      
        1 aggtcatccg tattcacgcc gattttctgg gcgtaaccga cgtccagagc atgttcggcg
       61 tcaatgtagg cggcgatgcc gtttttcttc tgcgcttcgg cgacgatgtg cagcgcaagt
      121 gtcgttttgc ctccggattc cggcccgtaa atttccacca cacggccgcg cgggacacca
      181 aacgttccca gcgcgatgtc gaggctcaag gagccggtgg aaatggccgg tacatccact
      241 ttttctgaac cgcccagttt cataatcgag cccttgccga actgtttttc tatctgagta
      301 atcgccaaat caacggcctt tgatctatcc atatccgcca taatacaatg ccctccttat
      361 tttcttttta tattaataaa tgcttaaaaa ttacaactct aatttatgaa aacaacttat
      421 gctcattata tcctcaaagc aaactccgcc agttttgtgt aaacagcgcc ccgcggcgca
      481 agtgcttatg tttggaccaa cttatttctg caaccagtgg ttgcagtttt tcatacgctt
      541 taatctattt ctccttcaaa taattctgat tagttatgat actgcggcct aattcttttt
      601 ccgtttcttt gcgcgccgta cctgccacac cgcctccgcg tttggccacc tttctatttt
      661 tttcgaaagt atccggtttt tctttttgcg atatttccgt ggtaactttt tcgccaagca
      721 ttgtaaaaat caactccaga tcagtcatgt agtcccgcag attctgattc tttttgaaca
      781 atccttttaa cttcttatag tccgctggcg ttatcccaaa agttgctttt gatatttcag
      841 ccgtaagaat ggcataatcc cgttcgctta taatgcctct ttctctccac tcatcggtca
      901 ggttttggcg aatggctatt ccgcgcaacc gtttatcgat ccaatccctg ggataacctt
      961 tttgttcata caataatttc atgcgttctt gcgccagttc aggattttca atttcctgta
     1021 cccgctcata cccgactctt gccagccaac gcttgaacgg ctcagctttc ggtgaaggaa
     1081 tggattggat gatacggaac atactttctg tgttggcgca gtcagtagca tattttttgc
     1141 cgtcggcagc ttctaatttc agaccgtgac aaaatgtcac gacctcactg ccttctacgt
     1201 tcaaacgttg tttcaatttt ctccaataag cgcctgcatc agggctgttc gtcaatgcgc
     1261 cgcaaatatc tataacagag aaataccatt cctcatcatg ccagattcgc ctgatctttt
     1321 tacttccaaa aacaactatt tgattgtgtt ccatcttctg taaatcattc actgtatttt
     1381 tcttttaacc tcctgcttaa ttcggagatg ctatacttat tttctggatt cccactcgga
     1441 ggagggaatg acagccgggg agtatgttcc caaatggaac atactgaact ttcatttcga
     1501 actgtttcaa aaaatgcaac agttcgaatg ttatccaccc aaagcaaact ctgccagttt
     1561 ggtgtaaaca gcgctctgcg gagaaagctt gctttgaaat aagaacaatt tatcggcgac
     1621 aaattctccg gctttaaacg aatcatattt tttcagcgct tcgctcatgc ctgtaatgtc
     1681 acgcgggtct ttgattcgcg ctaacgtcag atgcgccttg aaagaccggt cttcctcaag
     1741 aaagcctaaa gtcgcgaaat cggcatcgag ttttttctga agagtaataa gtttttccac
     1801 atcgcccgtt acaccgcacc acaaaacacg cggtctgcgg gcatccggga aaacacccat
     1861 cttttcaact ttcaaattca gtttctgttc ggcaacaact cgcttctgca cggtggcgca
     1921 aatactgttt atatcttcct tcgaaatatc gccgaaaaat tttaaggtca gatgctgacc
     1981 ctgcggcctt gtccagctga gccggccgtt gatttcccgt tttagttttt cctgaaggcg
     2041 ggagatttca ttaagaatat tttccggcgg ttcaatcgcc agaaaggcgc ggatattttt
     2101 ttcgttatcg gctgtcatac tctaaacttt ccttatgttc tgtcgtcttc ccggtcgtag
     2161 atgaccttta ggttacgaag gccgggaacc agttcataat tcctggatgc cggatcaagt
     2221 ccggcatgat aaaggataat tttcgtgacg cttcctatag tcagcgagtg tcattaagct
     2281 ttctcctgca aatcacgggg attatagtac cctccgctgt gcgggattgt tcgacttaca
     2341 aatttcaatt cacttcgttt ttgcaatttg agtctcacaa aagtttttca gcaaaaaaag
     2401 cgccgcttcg gagaaaacct ccttgttcct tttgcggtcc cagcgaaagg tgtaatggcg
     2461 gcaaatcgtc ttttgtgcat ccgcgactgc cagataaact gtgcccacgg gtttttcctt
     2521 agtgccgccg gtcggtccgg caat
//
LOCUS       DAOSME010000181         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_46581_length_2453_cov_12.500417, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000181 DAOSME010000000
VERSION     DAOSME010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46581_length_2453_cov_12.500417"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1443
                     /locus_tag="PLG98_12040"
     CDS             259..1443
                     /locus_tag="PLG98_12040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE34041.1"
                     /translation="MSDNRNPDLLIFEPQIAGHRLTWLQYITEDFLHAGYRVAWAIDS
                     RNGAKRAIQERLASAFDDIEAISVLDEKNGWRGGSKINAIAECLELSGAKHVFVNELD
                     EVASNIFRKAAVGVYPPENLKGRLSGVYFRPRFLTDPVWPPGNMIKAAGFRKLLEQEW
                     FENIYLVDEYLMASSRIRSAGHFHFLPDPWSGEYFCSSPNARRDIQIPADKFVFLHYG
                     IGDRRKGLHLAAAAMEKTPSDSRLFLLCAGQIDLEHSLLNHIAALEKKGKARLINRYV
                     SDEEERMIFAATDAVLLPYIHHYGSSGVLSRAAAAGKLVIASDEGLLARRVRDHGLGL
                     LFPTNDVPALQQRMTEAAALSREQRAGYQEKLLHYAGTCSRKNFRQALIAPWVRTGKT
                     DM"
     gene            1449..>2453
                     /locus_tag="PLG98_12045"
     CDS             1449..>2453
                     /locus_tag="PLG98_12045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOE34042.1"
                     /translation="MKNSPLLSKAPKKILLITTRRIGDVFLTAPLIASLREAYPDAKI
                     DMLVFKGTEGAVFANSALSRIVTVEEGAALPKQIKTVCRMFRKYDLALTTLTGDRPVF
                     YARIAGKKSVGFVENGIKHFWKRLLLNRYELFDNDDTHTVIMNLSLLKHLGIEPVCQL
                     AAVWTNADESAVRKLLPFDPAQKRYAVVHCYPMFPYKMWTFEGWKDIIFRLSDSLGLP
                     VILTGGNAADERDYISDLHRETAGRSIDVAGKISLGGVAFLLSKAAVYVGLDTAVTHM
                     AAALGVPTVALYGPTNPVKWGPMPGNHVFPGKSPYRMKGSQVVKNVFLLQGYGDCVPC
                     R"
BASE COUNT          569 a          613 c          678 g          593 t
ORIGIN      
        1 aaaaagatta aatatgtaat caaattgata ttataatgtc aattgctaag ttcccgtagc
       61 ggtgggcgct ccggcggaaa ttacctgttt atattcaaaa ttatttatgt taaatgcccg
      121 gatatacatg acggcgcctg cattactttt ttaaatcaat tgatttcccg gtgacggtat
      181 attcaggacc gtcgtaaatt gaaatatttc tcacccgtcc tgtttgcaga atgccgctga
      241 cgggaaaaag agcatgatat gtctgacaac cgaaatccgg atctgctgat attcgagccg
      301 caaattgccg ggcaccgcct gacgtggctt cagtatatta ccgaagattt tcttcacgcg
      361 ggataccgcg ttgcctgggc gatcgactcg cgaaacggcg cgaaacgcgc gattcaggaa
      421 cgtctcgctt ccgcgtttga cgatattgag gcgatttctg ttttggatga gaaaaacggc
      481 tggcggggcg gcagtaagat caatgccatc gcggaatgtc tcgagctcag cggggctaaa
      541 catgtttttg tcaacgaact cgatgaggtg gcctccaata tcttccgaaa agcggcagtg
      601 ggcgtttacc cgccggaaaa tctcaagggc agactcagcg gggtttattt ccgtccacgc
      661 tttttgaccg acccggtctg gccgccgggc aacatgatca aggccgccgg cttccggaaa
      721 ctcctggagc aggaatggtt tgaaaatatt tatctggtgg atgagtatct gatggcgtct
      781 tcccggatac ggtctgccgg ccattttcat tttttgccgg atccctggtc gggagagtat
      841 ttttgttctt ctccaaacgc aagacgagat atccagattc ccgcagacaa gtttgtgttt
      901 ctgcactacg gcatcggaga ccggcgcaaa ggcctgcatc tggccgcggc ggccatggag
      961 aaaacacctt ccgactcccg cctgttttta ttatgcgccg gacagattga tctggaacat
     1021 tccctgctta accatatcgc cgcactggag aaaaaaggga aggcccgact gatcaatcgc
     1081 tatgtgtccg atgaggaaga acgcatgatt ttcgccgcaa cggacgcggt tttgctgccc
     1141 tatattcatc attacggatc aagcggcgtc ctgtccagag cggcggccgc cgggaaattg
     1201 gtgatcgcct ccgacgaagg cctgctggcc aggcgtgtgc gggatcatgg cctgggcctg
     1261 ctttttccga ccaacgatgt tcccgccctg cagcagcgca tgacggaagc ggcggcgttg
     1321 agccgcgagc agcgagccgg gtatcaggaa aagctgctgc attatgccgg gacgtgttcg
     1381 cggaaaaatt ttcgtcaggc attgattgcg ccctgggtcc gtacgggaaa gacagatatg
     1441 taaaaacgat gaagaacagt cccttgttat caaaagcgcc gaaaaaaatt ctgttaatca
     1501 cgaccagacg catcggcgac gtttttctga cggcgccgct gattgcctcg ttgcgcgagg
     1561 cctacccgga tgccaaaatc gatatgctgg ttttcaaagg aacggaaggt gctgtttttg
     1621 caaacagcgc gctcagcagg attgtcacgg tagaggaagg cgccgcgctc ccgaaacaaa
     1681 tcaaaaccgt ttgccggatg ttcaggaaat acgatctggc cttaacgacg cttacgggag
     1741 accggccggt tttctatgcc cgcatcgccg gaaaaaaatc ggtcgggttt gtggaaaacg
     1801 gaataaagca tttctggaaa cggctgcttt tgaaccgcta tgagttgttc gataacgatg
     1861 acacgcatac cgtcattatg aatctttctc ttctaaagca tctggggatc gagccggttt
     1921 gtcaactcgc cgctgtgtgg accaacgcgg atgaatcggc ggtcagaaaa cttctgccct
     1981 tcgatccggc gcaaaaacgg tacgccgtgg ttcattgtta tcccatgttt ccctataaga
     2041 tgtggacgtt cgagggatgg aaagatatca ttttccggct gagcgattcg ctggggctgc
     2101 cggtgatact aaccggcggc aatgccgcag atgaacgtga ctatatttcc gaccttcatc
     2161 gggaaaccgc cggcagaagt atagatgtcg ccggcaaaat cagcctgggc ggcgttgctt
     2221 ttttgttgag caaagccgcc gtctatgttg gtctcgatac ggcggtcacg catatggccg
     2281 cggcactggg cgttccgacg gtcgcgctct acgggccgac gaatcccgtc aaatggggac
     2341 cgatgcccgg caatcatgtc ttccccggca agagtcctta ccgcatgaaa ggatctcagg
     2401 ttgtcaaaaa tgtttttctt ttgcaggggt atggggattg cgttccgtgc cgc
//
LOCUS       DAOSME010000182         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_47243_length_2428_cov_15.282764, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000182 DAOSME010000000
VERSION     DAOSME010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47243_length_2428_cov_15.282764"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLG98_12050"
     CDS             complement(<1..759)
                     /locus_tag="PLG98_12050"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOE34043.1"
                     /translation="MLFRLNASHCLALSLLLLFFSAAATESVSHAAEMIPESIISISS
                     GYVVAVDKKLQKLYVFKKNGDFSRVFEAGCSTGKNHGSKQLSGDAKTPTGIYFATRIL
                     TNPGPPETYGTLAFSLDYPTITDRKAGKNGTNIWIHGTTKPITPFQTNGCVVLKDKDI
                     QALAKFIHLNKTPVIIVESIRWIPQNKTTPVKQELEKVLSDWTKGYLDGNIKAMDKLY
                     LKNFQIAGKKRERLASHLSNAQNIKQHFVLEPRDI"
     assembly_gap    1096..1105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1106..>2428
                     /gene="pilM"
                     /locus_tag="PLG98_12055"
     CDS             <1106..>2428
                     /gene="pilM"
                     /locus_tag="PLG98_12055"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOE34044.1"
                     /translation="PFQVGVDIGANAVTMVKMGKTSDGKALLVDQKVVKLGEGISKKS
                     PEFNNLLKSSLEAFAGSLDDCDIWAMMSAAEVNVFRLKIPIVPANQMENVIYWAAKKE
                     NPIDEKEAFFDYEVQGKISDQGIPKYAVMVYSAPRSEPEKVKKLFSSIGIRLAGVTIA
                     PFAIQNYFRTEWITAGEGAFASIFIGHDFSRIDIYRKNDLVMTRGIKTGISSMMEAID
                     EAIAEAPSDAREEKERAKALLNEIAADPEKWMKEEGGVNWAETGILEMTTPALERLLR
                     QIERTMEYYATSVGFEKVEKVYLSSAISVFYYPLLHYLREQIGSISEYFDPFAGKSFP
                     VKSGLSSLAERALLIPAIGLALSDSKHTPNAIFTYAQKKQELIRQRINRGVFSAFAAA
                     LSVCIVIIFLQVGEARQLNLQRGKLEKELLLLGPKLSKDKIAEMAKEMK"
BASE COUNT          640 a          531 c          628 g          619 t
ORIGIN      
        1 aatgtctctt ggctccaaaa caaaatgttg tttgatgttt tgagcattgg acaggtggct
       61 cgcgagccgt tcccgctttt tacccgcaat ctggaagttt ttcagataaa gcttgtccat
      121 cgctttgatg tttccgtcca ggtatccctt tgtccagtcg gaaagcactt tttccagttc
      181 ttgtttcacc ggggtcgttt tattttgcgg aatccagcgg atggattcaa cgatgatgac
      241 cggtgttttg ttcaagtgaa taaatttggc cagcgcttga atatctttat ccttcaagac
      301 aacgcatccg ttggtttgaa aaggagtgat cggtttggtt gtgccgtgga tccagatatt
      361 ggttccgttc ttgcccgctt ttcggtccgt aatggtcgga taatccagcg aaaaggcgag
      421 ggttccgtag gtttcgggcg gtccgggatt ggtgaggatt ctcgttgcaa aataaatccc
      481 tgtcggagtt ttggcgtctc cggacaattg cttgctgccg tgatttttac ccgtagagca
      541 tccggcctcg aatactctgg aaaaatcgcc gtttttcttg aaaacgtaaa gcttctgcag
      601 ctttttatcc acggcaacca cgtacccgga agagatggaa atgatggatt cggggatcat
      661 ttcggcggca tgagaaacgc tctctgtagc agcggcggaa aaaaacagca ggagaaggga
      721 cagggcaaga cagtgggacg cattcaatct gaaaagcata aatttacctt attgtgtatt
      781 ttcgaggcgc tgccttttta gcaaaaaccg atatgcgttt caagttcttt cgccgtgtgc
      841 aaaaccaaat ataaacattt gtaataatta tcgaagtgtt gtatgaaaaa aacatttata
      901 aaaagtaact attgttcggg gtagaatcaa catttattgg agatatttgg ctggcggttt
      961 gtcgcaaaaa aggttgtttt ttgggtgagc tacttggaaa agacaaaaga aattaactca
     1021 acggaaaagt tgttaaacgt cattcgcggc gcgaaaccgc cttttccggc aacgccgggc
     1081 agcggtccga atattnnnnn nnnnnccttt tcaggtaggc gtggatatcg gggccaatgc
     1141 cgtcacgatg gtcaaaatgg gaaaaacctc tgacggcaag gccctgctgg tcgatcaaaa
     1201 agtggtgaaa ctcggtgagg gaatttccaa aaaatcccct gaattcaaca atctgcttaa
     1261 atcctcgctg gaggcgtttg ccggatcact ggacgattgt gatatctggg cgatgatgtc
     1321 cgccgcggaa gtgaatgttt tccgcctgaa aataccgatt gtccccgcaa atcaaatgga
     1381 aaatgtcatc tactgggcgg caaaaaagga aaatccaatc gatgagaagg aagccttttt
     1441 tgactacgaa gttcagggaa aaataagcga tcagggcatt cccaaatacg ccgtcatggt
     1501 gtacagcgca ccccggtccg agccggagaa agtaaaaaaa ctgttttctt ccattggcat
     1561 tcgacttgcc ggagttacca tcgcgccttt tgccattcaa aattatttcc ggacggagtg
     1621 gatcacggcg ggggaaggcg cctttgccag catttttatt ggacatgatt tttcgaggat
     1681 cgacatctac cggaaaaatg acctcgtgat gacacggggc atcaagacag ggatcagcag
     1741 catgatggag gccatcgacg aagccattgc ggaagcgccg tcggatgcca gggaagagaa
     1801 ggaaagagcc aaagccttgt tgaatgaaat tgccgccgat ccggaaaaat ggatgaaaga
     1861 ggaaggcggc gtcaactggg ccgaaaccgg aatcctggaa atgacgactc ccgccctgga
     1921 gcgactgctg cgtcagatcg aaagaacgat ggaatactac gccacctccg tcggttttga
     1981 aaaggtggag aaagtatatc tttcgtcggc gatcagcgta ttctattatc ccctgctgca
     2041 ttacctcagg gaacagatcg gttccatcag tgaatatttc gatcccttcg cgggcaaatc
     2101 atttccggtt aaaagcgggc tttcgtctct ggcggaaagg gccttgttga ttccggccat
     2161 tggactggcc ctgtccgaca gcaagcacac gccgaatgcc atctttacct acgcgcagaa
     2221 aaaacaggaa ctgattcgcc agcggatcaa ccgcggcgtt ttttcggcgt tcgcggccgc
     2281 tttgtccgtt tgcatcgtca tcatttttct tcaggtcggt gaagcacgac aactgaatct
     2341 ccagagaggg aaacttgaaa aagaactctt gctgttgggc ccgaagttgt ctaaagataa
     2401 aattgctgaa atggccaaag aaatgaaa
//
LOCUS       DAOSME010000183         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_48628_length_2374_cov_16.181544, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000183 DAOSME010000000
VERSION     DAOSME010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48628_length_2374_cov_16.181544"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..524)
                     /locus_tag="PLG98_12060"
     CDS             complement(213..524)
                     /locus_tag="PLG98_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HOE34045.1"
                     /translation="MKKFSIFIAVIAVIALMGCATMIPIGGLYTATKLPGMATSNTGA
                     ALKTGEAQCTSILGLVAYGDCSISTAKKNGKIKTVHSVDWDAKNILGIYGTYKVIVTG
                     Q"
     gene            complement(794..1426)
                     /locus_tag="PLG98_12065"
     CDS             complement(794..1426)
                     /locus_tag="PLG98_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOE34046.1"
                     /translation="MCGRFALVTDLKSIQKHFNIQEVFCQYQPSWNVLPGQLIPAVIN
                     DHGKKRLVCFRWGLIPSWSKNPSPADGLINARAETVDRKPSFKDALKKRRCLIVADGF
                     YEWKKEDNKKIPFYYYLKSGRPFGFAGLYDTWLSPDKKEISTCTIITTAANEFIFPVH
                     DRMPVILSPEREKIWLAGETNDVSVLKSVLKPYPSGEMDAKPGQGPGSIK"
     gene            1831..>2374
                     /locus_tag="PLG98_12070"
     CDS             1831..>2374
                     /locus_tag="PLG98_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOE34047.1"
                     /translation="MKKRMDIHAVEQKFAVFFREQRRMPTYSEMLPLLGVKAKSAAEY
                     WTKKLLAKGALEKDAKGFLKPAHWSLNLPLVGNVAAGFPSPAEEELRDTISFDEYLVN
                     NRASSFVLSVTGDSMIGEGIKEKDLVIVERGREPKNGDIILAEVDGRWTMKYFHKKGK
                     KVTLEAANPKYPPIIPQEELR"
BASE COUNT          596 a          552 c          583 g          643 t
ORIGIN      
        1 aattcattgt gtgtctcctt ttatcaataa tctgtttgcc gtgaaggctt taggggtaca
       61 tattgcagaa atgtatagaa gaagtaacaa aatctgtctg gcgaagtcaa gcgaaaaccg
      121 gcacaggaga tgatctgaaa gatgttgatc agggcctgtg ccggtcaaga cggacaaggt
      181 tcattttaga aaacaatttt ccttccggtt ttttactgac cggtaacaat cactttgtac
      241 gttccgtaaa ttccgagaat gtttttagca tcccagtcaa cgctgtgcac ggttttgatc
      301 ttgccgtttt tcttggcggt actgatgctg cagtcgccgt atgcaaccag tcccaggatg
      361 ctggtacact gggcttcgcc ggtcttgagt gccgcgccgg tattgctggt tgccatcccc
      421 ggcagttttg tcgccgtgta gagtccgcct atcggtatca tggtggcgca ccccatcagc
      481 gcgataacgg caatgaccgc aataaaaatt gaaaattttt tcatggcacc tccttgagaa
      541 tttaaggttt aaattgagtt aacctcttgt acggatagct gtttgttaaa ccagactcgg
      601 ataaatcaat cttcctaaaa tttaccacca ttccttttga tattcaagag ttttacatgg
      661 aaattttaca aggttataaa ttttttatgg agaaaaaaat ttctttgtga tgaaatgccg
      721 tccggctttc cgcaaaagag ccggaagaaa aatcgtagta agatgcacgt catcccgtaa
      781 atatagaaaa agattatttg atgcttcccg gaccctgtcc cggttttgca tccatttctc
      841 cggagggata gggtttcaga acagatttga gaacggaaac atcgtttgtc tcgccggcca
      901 accagatttt ttcccgttcc ggagacaaga tcaccggcat gcggtcatgc acggggaaaa
      961 tgaactcgtt ggcggccgtg gtgataattg tgcaggtgct gatttctttt ttgtccggcg
     1021 acagccatgt gtcgtaaagt cccgcaaacc cgaagggccg tcctgatttt aggtaataat
     1081 aaaacggtat tttcttgttg tcttctttct tccactcgta aaaaccgtcg gcgacaatca
     1141 ggcagcgcct ttttttaagc gcatccttaa aactgggttt ccggtccacg gtttcggcgc
     1201 gggcgttaat caagccgtcg gcaggggagg gattcttaga ccatgaagga atcaggcccc
     1261 agcggaagca gaccagccgt ttcttcccgt gatcgttgat gacggcgggg atgagctggc
     1321 cgggcagaac gttccaactg ggctggtatt ggcagaagac ctcctgaata ttgaaatgct
     1381 tctggatact cttcaaatcg gtgaccagtg caaatcgtcc gcacatactt ttatccttaa
     1441 gttgcttgca gtcataccgg cgcaggccgg aatccagtca gttaaaatta taccagattt
     1501 cccggctgac cgtcctttta aatttgagcg tcattaaaac tcgggcatca tccccattcg
     1561 ctccgcttcg gggataattt ccaatcccga tataccggga ttggataatt ccaccaacgc
     1621 ttcgcgtgtc gtttctctcg cgctcagcgg gtgtcattac cacttacgct ttaatgagcc
     1681 ttgattttcc gactcgcagt gatcggaaaa tcttagagcg aattatcccg ccacctttag
     1741 gtggcaggga caaactgcac tgcgtttgtt tttggcgagt ctcatcattt tttcttgaca
     1801 ataggacgag cgtcctatat gctgctttcc atgaagaaaa gaatggacat ccatgcggtg
     1861 gagcaaaaat ttgccgtatt tttcagagaa cagagaagaa tgcccaccta ttcggaaatg
     1921 cttccgcttc tgggcgtcaa ggccaaaagc gccgccgaat actggaccaa gaaactgctg
     1981 gccaaaggcg ctctggaaaa agatgccaaa ggatttctga agccggcgca ttggtctttg
     2041 aatcttcccc tggtgggcaa tgtggcggcc ggctttccct ctcctgcgga agaagaactg
     2101 cgcgatacga tttctttcga tgaatatctg gtcaacaacc gggcgtcgtc ttttgttctc
     2161 tctgtgaccg gcgattccat gatcggcgaa ggcatcaaag agaaagacct ggtcattgtg
     2221 gagcgcggcc gggaaccgaa aaacggggat atcattctgg cggaagtgga cggtcgctgg
     2281 accatgaagt attttcacaa aaaagggaaa aaagtgacac tggaagcggc caacccgaag
     2341 tatccgccga ttattcctca ggaagaatta cgta
//
LOCUS       DAOSME010000184         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_48833_length_2367_cov_17.734429, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000184 DAOSME010000000
VERSION     DAOSME010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48833_length_2367_cov_17.734429"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1062
                     /locus_tag="PLG98_12075"
     CDS             283..1062
                     /locus_tag="PLG98_12075"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOE34048.1"
                     /translation="MINVNTENHIMLIEVDRVKKLNALTIEMYSQMAKAYYELDTNDD
                     LWVGLIYAKGPHFTSGIDLAAWSEQFAKGTGNPVAPEGEIDPYAMNNAVYCRKPLVMA
                     VQGYCFTWGFELLLNTEIRVAATDTRFCAMEVRRGFFPTGGGTLRLPKEIGWGNANRY
                     ILTGEEMTAEEAYRLGLVQFLTEPGKQFDKALEVTRRIASVAPLGVQGALKHSRITYL
                     KGEDAVKDVLWEDTSAIMKSEDMKEGVQSFLERRPAVFKGK"
     assembly_gap    1076..1175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1189..2226
                     /locus_tag="PLG98_12080"
     CDS             1189..2226
                     /locus_tag="PLG98_12080"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOE34049.1"
                     /translation="MKTRLTEMVGIKYPIVLSGMSWISVPKMVAAVSNAGGLGILATG
                     PLNAGQTREAIREIRKLTDKPFGANASLMFPGAVENAKVLLQEKVPVINFALGKGDWI
                     VNEAHKYGGKVFATVVNLKHAKRAQEFGTDAVIATGQEAAAHGEDVTSLVLIPHLAEN
                     LKIPVIAAGGFADGRGIAAALALGAEGVAMGTRLMTTKESPLHENSKKLTQEKDVYDT
                     LFSERFDGILCRVMKTDTAKKAMKKGLNWPAAFFNSQAIAKQMDIPYFKLFIGVLLSG
                     YKNAKQMAYMANAHKAFKTATEDGDTKIGLLPVGQVQGLIHDQPTVSELFERIMKEAK
                     AAQAKVNSALE"
BASE COUNT          648 a          510 c          604 g          505 t
ORIGIN      
        1 gattacgcgc ctttgatggt cggccagtcc atcggcctga tcaacgatat ccccacctgc
       61 gcggagctgc tggaaagaat ggcggcccag gccaaagaaa tctctgcaaa agcatcaaag
      121 ctctgtggaa actgatacca ggaagaatta agtaaactta agtcgaagcg caactcatca
      181 gaaaaggaca caaggaagga tacgaattac atgaaatttg aagtctttcc gaataattgg
      241 cagtaaataa attttttgta aatactttcg aaggaggata atatgattaa tgttaatacc
      301 gaaaaccata taatgctgat tgaggtcgat cgcgtaaaaa aattaaacgc gctgacaata
      361 gaaatgtatt cccaaatggc gaaagcctat tacgagctgg atacgaacga tgatttatgg
      421 gtcggactca tttatgccaa aggcccccat tttacttctg gaattgatct cgctgcttgg
      481 tcggagcagt ttgcgaaagg cacgggcaat cctgtggctc cggaaggtga aattgaccca
      541 tatgccatga acaatgcggt atattgccgc aaacctcttg tcatggccgt gcagggctat
      601 tgttttactt ggggttttga actgctgctc aatacagaaa tccgggtagc ggccacggac
      661 acacggttct gtgcgatgga agtgcgccgc ggatttttcc ctaccggtgg cggaacttta
      721 cggttgccca aagaaatcgg ttggggtaac gccaatcgtt atatattaac cggcgaggag
      781 atgaccgcag aggaggccta tcgtttaggg ctagtccagt ttctgaccga accagggaag
      841 cagttcgaca aagccctcga ggttacccga cgtattgcga gcgtcgcccc tctgggtgtg
      901 caaggtgcat taaaacacag ccgaatcacc taccttaaag gtgaagacgc cgtaaaggac
      961 gttttatggg aagatacgtc cgccattatg aaaagtgaag atatgaagga aggggtacag
     1021 tcttttctgg aacgccgtcc tgccgtgttt aaaggaaaat agctttcact ttatcnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaag gagaaattat gaaaactaga
     1201 ttaacagaaa tggtcggtat taagtacccc atcgtgcttt ccggcatgag ctggatcagc
     1261 gtgcccaaaa tggtggcggc ggtttccaat gccggaggct tgggcattct ggccaccggt
     1321 ccgctgaacg ccggacagac ccgggaagcc attcgcgaaa tccgcaaact gacggataag
     1381 ccgttcggcg ccaatgcgtc gctgatgttt cccggcgcgg tggaaaacgc caaagtcctg
     1441 ctgcaggaaa aagtacctgt cattaacttt gcactgggca aaggcgactg gattgtaaac
     1501 gaggcacata aatacggcgg aaaggttttt gccacagttg ttaatctgaa gcatgccaag
     1561 cgcgctcagg aattcggcac ggacgcagtg atcgccaccg gacaggaagc ggcggcgcac
     1621 ggcgaagacg tcacctcgct ggttttgatt ccgcatctgg cggaaaatct gaaaattccc
     1681 gtcattgcgg ccggtgggtt tgccgacggc cgtggcattg cggcagcgct cgctctgggc
     1741 gcggagggag tcgcgatggg aaccagactg atgacgacga aagaaagtcc actgcatgaa
     1801 aactcgaaaa aactgacgca ggaaaaagac gtttatgata cgctgttttc tgaacgcttt
     1861 gatggcattt tatgccgggt catgaaaacg gatacggcca aaaaagcgat gaaaaaaggc
     1921 ctaaactggc ctgctgcatt tttcaactcc caggccatcg cgaagcagat ggacatcccg
     1981 tattttaaac tctttatcgg ggttctgctg tccggttaca aaaatgcaaa gcagatggca
     2041 tacatggcca acgctcacaa agcgtttaag acagccacgg aagacggtga taccaaaatc
     2101 ggtctactgc cggtgggaca ggtacaggga ttgattcatg accagccgac ggtttccgaa
     2161 ctttttgaac gaatcatgaa agaagcaaaa gccgcacagg ccaaagtaaa ctcggcgctc
     2221 gaataaaacg gcgttgccga aaagctgtaa aaagttgatg atgcattcac gcaaaatgga
     2281 aaggagtaat tttatatgtc catcgaatcg gtactaaaag aattgaagcc gcacctcggg
     2341 aaggaagttt ttgtctccga ctggaaa
//
LOCUS       DAOSME010000185         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_49175_length_2355_cov_24.288261, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000185 DAOSME010000000
VERSION     DAOSME010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49175_length_2355_cov_24.288261"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..433
                     /locus_tag="PLG98_12085"
     CDS             62..433
                     /locus_tag="PLG98_12085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOE34050.1"
                     /translation="MNEKRKASRLMEESEVTITLIAGGKKFFREKVIYNSSKDISESG
                     ARVHSSIFLPIDTLLKIDFKLKNLQHMITAMGKVKWIKIICEGEAYDAGVEFVNTPTE
                     AIRKIADYIAWRQKQKTTKRH"
     gene            586..780
                     /locus_tag="PLG98_12090"
     CDS             586..780
                     /locus_tag="PLG98_12090"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOE34051.1"
                     /translation="MEQNMGTVDKVIRTIVAILIIVAYLMGMLPGIAGLLLIISGSLL
                     SSVVSGYCPLYTKIGVNTNR"
     gene            894..2273
                     /locus_tag="PLG98_12095"
     CDS             894..2273
                     /locus_tag="PLG98_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOE34052.1"
                     /translation="MNIAVLNGSPKGDVSVTMQYVHFLQQRFPETEFRIFDVALKIKK
                     LENDDRAFQEVIANVADADAVLWAFPLYFLLVCSQYKRFIELIFEKNAVSAFHGKPAA
                     CLTTSINFFDYTAHNYIHAVSEDLGMNYFGSYSAEMNDLLKTKERRRLKTFGELFLKA
                     IREKTPAIRSYKPLVAPEVVFDTEAIRPVDAAGKKVLILADDLAANSNLRKMVERLQK
                     TFSPAAELVQLKDVDIKGGCLGCIKCGFDNVCVYEGKDGYIDFFNHKIKPADIVFLAG
                     AIHDRYLSSLWKCFFDRSFFNGHTPFLKDKQIGFVISGPFGQIHNLREIFDATTKFQL
                     AGLAGFVSDDTGGNPEINAALQTLAVQAAEQSRLGYIQPPTFYQVAGAKLFRDVIYGR
                     LRPVFQADHRYYKEHGMYNFPQKYYKWRMINSTLVFLTKIPAFRREFFKRIKKEMIKP
                     AAGALKKTE"
BASE COUNT          652 a          505 c          585 g          613 t
ORIGIN      
        1 tttttcggtt ctcgaaagtg taattaaccc ggaaacgatt acccaaaagt aacggattgt
       61 tatgaacgaa aaaagaaaag ccagccgcct gatggaagaa agtgaggtaa caatcaccct
      121 gattgccggt ggcaaaaagt ttttcaggga aaaagtcatt tataacagca gcaaggatat
      181 ttcggaatca ggcgccaggg ttcactccag catatttttg ccgatcgaca ctctcctcaa
      241 gatagatttt aaattaaaaa accttcaaca catgattacc gccatgggta aggtgaagtg
      301 gatcaaaatt atctgtgagg gcgaagcata tgatgccggg gtggaattcg tgaacacgcc
      361 gacggaagcc atcagaaaaa tagcggatta cattgcatgg cggcaaaagc agaaaaccac
      421 aaaacgtcat tgaccctatt ggctcccgtt gtttgaatac gttccggaag gcggtcttgc
      481 cgtgaaagtg taatgtgcgg catcggtttg gtgtgaaaac ggcagatggt tgattccggc
      541 atcttgaaat gtccggaaga agagaaatca tcatcaggag ggatcatgga acagaatatg
      601 ggtaccgttg acaaagtgat cagaacgatt gtggcgattc tcattattgt cgcctatctt
      661 atgggtatgc tgccgggaat cgcggggctg ctgctcatca tcagcggatc gcttctctcc
      721 agtgtggtaa gcggatactg cccgctttat acaaagattg gagtgaacac gaaccggtaa
      781 tccgcctgtt tgcaatcgat tgcgcaagct gttttaccgg gtattttctt gagccgagac
      841 cctccctgtg ataagagtcc cgatgaatcc catatggaat aaaaggtgat aatatgaaca
      901 ttgccgtttt aaacggttcg ccaaaaggtg acgtcagcgt gaccatgcag tatgtccatt
      961 tccttcagca aagatttccg gaaacggaat ttagaatttt tgatgtggca ttgaaaatta
     1021 aaaaactgga aaacgacgac cgggcgtttc aggaggtcat tgcgaacgtg gcggatgcgg
     1081 atgctgtgct ctgggcgttt cccctttatt ttcttctggt ctgttcgcag tataaaagat
     1141 tcatcgaatt gatttttgaa aaaaatgccg tttccgcttt tcacggcaag ccggcggcct
     1201 gtctgacaac ctccatcaat ttcttcgact atacggccca taattatatt catgctgtca
     1261 gcgaagatct gggaatgaat tatttcggtt cctattcagc ggaaatgaat gacctgctga
     1321 aaaccaagga gcgccgccgc ctgaaaacgt tcggtgaatt gtttctaaaa gcaatcagag
     1381 aaaaaacgcc ggcaatcaga agctacaagc cgctggtcgc tcctgaagtc gtttttgaca
     1441 ccgaagcgat tcgccctgtg gatgcggccg gtaaaaaagt tttgattctg gcagatgatc
     1501 ttgcggcaaa ttccaatctc cggaagatgg tggagcgatt gcagaaaacc ttttctcctg
     1561 ccgccgaact ggtgcaactc aaggacgttg atatcaaagg aggatgcctc ggctgtataa
     1621 aatgcggttt cgataatgtt tgtgtttacg agggcaagga cggttacatc gattttttca
     1681 atcacaaaat taagccggcc gatattgtgt ttctggccgg cgccattcat gaccgttatc
     1741 tgtcttcgct gtggaaatgc ttttttgacc gcagtttttt taacgggcat acaccgtttc
     1801 tgaaagacaa gcagatcgga tttgtcattt ccggtccgtt cggacagatt cataatttgc
     1861 gtgaaatatt tgatgcgacc acgaaatttc aactggccgg tcttgccggc tttgtcagcg
     1921 atgacacggg cggaaatccg gaaatcaacg cagccttgca gactctggcc gtccaggcgg
     1981 ctgagcagag ccggctcggt tatattcaac cgccgacatt ttatcaggtt gccggcgcga
     2041 aattattccg ggatgtcatt tacggcaggt tgcgccctgt ttttcaggcc gatcaccggt
     2101 actacaagga acacgggatg tacaattttc ctcaaaaata ttacaaatgg cgaatgatca
     2161 acagtacgct ggttttcctt acaaaaattc cggcgtttcg ccgcgagttc ttcaaaagaa
     2221 taaaaaagga aatgataaaa cccgcagccg gcgcgttgaa gaaaacagaa tgatggttgt
     2281 tgcatctgaa aagcatctat ttcaataaaa ctctggagga aacatgaatc atcaggatgt
     2341 cgtggcgttc gtgac
//
LOCUS       DAOSME010000186         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_49306_length_2350_cov_19.376035, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000186 DAOSME010000000
VERSION     DAOSME010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49306_length_2350_cov_19.376035"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2350
                     /locus_tag="PLG98_12100"
     CDS             <1..>2350
                     /locus_tag="PLG98_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE34053.1"
                     /translation="IMALRQQSPIRLLIADDVGVGKTIEALLIIKELLERREINRFAI
                     IALPHLCEQWQGELKDKFGINAVIIRSNTQARLDREIQGDRSVYDYYPYQIISLDYIK
                     SDQRRQVFIQEAPELVIVDEVHTCAKPTGANVAQQQRHHLVYDLAQKKNQHLILLTAT
                     PHSGKAEQFSSLLGLLKEEYSLLDLPQATQNQRKELAEHYVQRRRADVLKWMDEDTPF
                     PKRDPGEYQYDLSPAYSAFYENVLNFALGLIKGTEQKAGRARYRYWSVLALLRGVMSS
                     PATGLEMITNKITGKETDLPDEADVLPDAETNPIMDEDYGAAKDFTPTTVFEKTDWST
                     SENKQLANLAAQIHNLHGLEYDKKAVRAQEIVSSWIKEGFNVVIYCRFIATANYLGEV
                     LKPSLQKIHSDINIQVVTSEDPDEVRKSRIDDMESSKHRVLIATDCLSEGINLQDKFT
                     AVLHYDLPWNPNRLEQREGRIDRFGQTAPIVKAYLLYGNNNPIDGVVLKVLLRKVREI
                     RKATGISIPFPEDSQSLIDSVLQAVLLSAPAQTQSQQLTLQFNNADEVQEKELIATKA
                     IEAAAARETASRNIFAQHAIKAHEIEQDLQQSDEAIGTPQAVESFVTDALNHLLGAQI
                     TKDKKGYVLFTANLPPVLKDTLPQTAQLKVSFYSPTPEGYLYIGRNHLFVEQLCQHLM
                     AHSLKQDKHYGPARASVIRCRYVAIKTTLLLFRVRNVIEEKQTGKQLVAEEMLTWGYQ
                     GNAGDNQILSADEIKHLMENAVPTANLTEQAKEEFLTNELGR"
BASE COUNT          666 a          578 c          573 g          533 t
ORIGIN      
        1 tgattatggc gctcaggcaa caaagcccca ttcggcttct cattgccgat gatgtcggcg
       61 tcggcaaaac cattgaagcc ctgcttatca ttaaagagct tttggaacgc agagaaataa
      121 atcgtttcgc cattatcgcg ctgccgcacc tgtgtgaaca gtggcagggg gaattaaaag
      181 acaaattcgg cattaatgcc gtgattatcc gttccaatac acaggcgcgt cttgaccggg
      241 aaattcaggg cgaccgcagc gtttatgact attacccgta tcaaattatt tccttagact
      301 atatcaaatc ggatcagcgt cgtcaggtct tcatccagga agcccctgaa ctggtaattg
      361 ttgacgaagt gcacacctgt gctaaaccga ccggtgcaaa tgtcgcgcaa cagcagcgac
      421 atcatcttgt ttatgatttg gcacaaaaga aaaatcagca tttaatcctg cttacggcaa
      481 cgccgcacag tggcaaggcg gagcagttca gttctctatt ggggttgttg aaagaggaat
      541 acagcctgct tgatctgccg caggcgacgc agaaccagcg taaggaattg gcggaacatt
      601 atgttcagag gcgtcgggcc gatgttttaa agtggatgga tgaagacaca ccctttccta
      661 aacgtgatcc aggagaatat cagtatgatc tatctcctgc ctattccgcc ttttatgaaa
      721 acgtgcttaa ttttgctctg ggcttgatca aaggcacgga acagaaggct ggcagggcaa
      781 gataccgcta ctggtcggtg ctggctcttt tacgcggcgt gatgtccagc ccggcaacag
      841 gtctggagat gatcactaac aaaataactg gaaaagaaac cgatcttccg gatgaggcgg
      901 acgtcttacc cgatgccgaa acaaatccca ttatggacga agactacggc gcggcaaaag
      961 actttacacc caccactgtt tttgaaaaaa cggattggtc aacctcggag aacaagcaac
     1021 tggctaatct ggcagcccag attcataatt tgcacggtct ggaatacgac aaaaaagctg
     1081 tgcgcgccca agaaatcgta agctcgtgga taaaagaagg attcaatgtg gtaatttatt
     1141 gccggtttat cgccacggcc aattaccttg gcgaagtgct caaaccgagc cttcagaaaa
     1201 tccattcgga catcaatatc caggttgtga ccagtgaaga tccggatgaa gtgcggaaaa
     1261 gccgcattga cgacatggaa tcatcgaaac atcgggtatt gattgccacg gattgcttga
     1321 gcgaaggcat caacctccag gataaattta cagccgtgct tcattacgat ttaccctgga
     1381 atcccaaccg gctcgaacag cgcgaaggcc gcattgaccg cttcggacag actgcgccga
     1441 ttgtaaaggc ctatttgctt tatggaaaca acaaccccat cgacggcgtt gttttaaaag
     1501 tgcttttgcg caaagtgagg gaaatccgta aggcaaccgg tatatccatc ccctttccgg
     1561 aagacagcca gtcgttaata gattcagtgc ttcaggcggt gcttttgagc gcgccggcac
     1621 agacccagag ccagcagctt acattacaat tcaataatgc ggatgaagtt caggaaaaag
     1681 aactgattgc caccaaggcc attgaggctg cggcagcccg tgaaacggcg tcccgcaaca
     1741 ttttcgcgca gcacgccatc aaggcgcacg agatcgaaca ggatcttcag caatctgatg
     1801 aagccatcgg aacccctcag gcagttgaat cattcgtgac agacgcattg aatcatctgc
     1861 ttggtgctca aatcacaaaa gacaaaaaag gatatgtcct tttcaccgca aatctgccgc
     1921 cggttttaaa agacacgctc ccgcagacag cccagcttaa ggtaagcttt tattcgccaa
     1981 caccagaagg ctacctttat atcgggagaa atcatctctt tgtggagcaa ctctgccagc
     2041 atctgatggc ccattcttta aaacaggaca aacactatgg tccggcacgt gcctcggtga
     2101 tccgctgccg gtatgttgca atcaagacca ccttgcttct tttccgtgtg cgcaatgtca
     2161 ttgaggaaaa gcagacgggt aaacaactgg ttgccgaaga aatgctgacc tggggctatc
     2221 agggcaatgc cggagataat caaatcctct cggctgatga aataaagcat ctcatggaaa
     2281 atgctgtgcc cacagccaat ctgaccgaac aggccaaaga agagtttctg accaatgaac
     2341 tggggagaat
//
LOCUS       DAOSME010000187         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_49656_length_2338_cov_26.622865, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000187 DAOSME010000000
VERSION     DAOSME010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49656_length_2338_cov_26.622865"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /gene="rfbB"
                     /locus_tag="PLG98_12105"
     CDS             complement(<1..751)
                     /gene="rfbB"
                     /locus_tag="PLG98_12105"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOE34054.1"
                     /translation="MKNVLVTGGCGFIGSNFVRYTLQQYPEYRIVNLDKLTYAGNPGN
                     LVDLEKNPQYRFIQGDITDAVLVRKIFAEEQMDTVVHFAAESHVDRSITGPAEFVQTN
                     IVGTFTLLEAAREAWLADLNKANKSYRFLHVSTDEVYGSLGDTGSFQETTPYDPRSPY
                     SASKASSDHLVSAYFHTYGLPTLITNCSNNYGPYQFPEKLIPLVINNALQGKALPVYG
                     DGKNVRDWLYVVDHCEAILTVLQKGRIGETYN"
     gene            1016..2197
                     /locus_tag="PLG98_12110"
     CDS             1016..2197
                     /locus_tag="PLG98_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOE34055.1"
                     /translation="MYTGPLVFTQVMDFMPLKTFQRCVEKYQGNFSVKNFTCLDQFRI
                     MAFAQLTYRESLRDIEACLRAQSNKLYHMGIRSKVSRSTLAEANETRDWRIYADFAHH
                     LIGIARKLYQKEPLAVELQNTVYALDATTIDLCLSIFPWARFRTAKGAVRLHTLLDLR
                     GNIPSFIHISDGKLHEVNVLDIIPLEAGAFYIMDRGYTDFFRLYAVTQASAFFVIRAK
                     SNLKSRRLYSHSADKSTGVVCDQSILLTVVKSAGDYPDKLRRVRYYDAETNKTLVFLT
                     NNFLLPAITIAQLYKQRWQVELFFKWIKQHLRIKSFFGTSENAVKTQIWIAVSVYLIV
                     AIIKKRLNLQESLYTILQVLSISLFERASMFQLLTFYDYTSNMDENSNQLNLFTDYSG
                     Q"
BASE COUNT          607 a          584 c          543 g          604 t
ORIGIN      
        1 tgttgtaggt ttcgccgatt ctgcctttct gtaaaacggt gaggatggct tcgcaatggt
       61 cgaccacgta aagccagtcg cggacatttt taccatcacc gtacaccgga agcgccttcc
      121 cctgaagggc gttgttgatc accagaggaa tgagcttttc cggaaactga tacgggccat
      181 agttgttgga gcagttggtg attaatgttg gcaagccgta agtgtggaaa tacgcgctga
      241 ccaagtgatc ggatgaggct ttgctggccg aataaggaga ccggggatcg taaggggtgg
      301 tctcctgaaa ggaacctgta tcccccaaag agccataaac ttcgtctgtg ctgacgtgca
      361 aaaagcggta gcttttgttt gccttgttca aatccgcaag ccaggcttcc cgcgcggctt
      421 ccagaagagt aaaggtgcca acgatattgg tttgaacaaa ttccgcaggc cctgtaatgc
      481 tgcgatccac gtgggattcg gcggcaaaat gcacaacggt atccatttgc tcttcggcaa
      541 agattttcct gaccaaaacg gcgtccgtaa tgtcgccttg aataaaacgg tattgcggat
      601 tcttctccaa atcgactaga ttgccggggt taccggcata ggtcaactta tccaaattga
      661 cgattctgta ttccggatac tgttgcagag tgtagcggac aaagttggag ccgataaacc
      721 cacaacctcc tgtaactaat acatttttca taattttttc cccctgttca gtctttcagg
      781 atctgatccg actctcaaaa ggcggaagtc agccccaaac tttctcaaat tgcaggtgat
      841 tcattagcat aatttttgat aatcaggttc actactgtcc ggaatagtta gtatataaaa
      901 tttgtaagag cttaacatat ttcattaaag ataccattta tgtttgtcgt cgacttgaaa
      961 atgcataacg gaatttgtac aacttcccga agaatatttc cggggaggag tgctcatgta
     1021 tacaggcccg cttgtattta cccaagtcat ggacttcatg ccgttgaaaa cctttcagcg
     1081 ctgcgtggag aagtaccaag gcaacttcag cgtcaaaaat ttcacctgcc tagaccagtt
     1141 ccgcattatg gctttcgcac aactgacgta tcgggagagc ctgcgggaca tcgaagcctg
     1201 ccttcgggca caaagcaaca agctatatca catgggcatc cgcagcaaag tttcccgcag
     1261 caccttggcg gaagcgaacg aaacgcggga ttggcgtatc tacgccgatt ttgcccatca
     1321 tctgatcggc atcgccagga agctctacca gaaggaaccg ttggcagtgg aacttcaaaa
     1381 cacggtttac gccctggatg caacgacgat cgatctgtgc ctgtccattt ttccctgggc
     1441 acgcttccgg acagcaaagg gagcagtccg acttcatacc ctgctggatt tgcggggcaa
     1501 tatcccgagc ttcatccaca tctccgacgg aaaactccac gaggtcaatg ttctggacat
     1561 cattcctctg gaagcaggcg ctttctacat catggatcgc ggctacacgg atttcttcag
     1621 actctacgct gttactcaag cctccgcttt cttcgtgatc cgagccaagt ccaatctcaa
     1681 gagtcgcagg ctttattccc attcagcgga caaatccaca ggggttgtgt gcgatcaatc
     1741 catcctgcta accgttgtga aatcggctgg ggattatccc gacaaactac gacgggtgag
     1801 atattacgac gccgagacca acaaaacatt ggtgtttctg accaacaact ttctgctgcc
     1861 cgccatcacc atcgcacaac tctacaaaca acgatggcaa gtagagcttt tcttcaaatg
     1921 gatcaagcag cacctgcgca tcaaaagttt tttcggcact tcggagaacg ccgtgaagac
     1981 ccaaatctgg attgctgttt ccgtttatct gatcgtggcg atcatcaaga aacggctgaa
     2041 tctccaggaa agtctctaca caattttaca ggttttgagt atctccttat ttgaaagagc
     2101 gtccatgttt cagctactta cattttatga ttacacaagc aatatggacg aaaatagtaa
     2161 ccagttgaac ttattcacgg attattccgg acagtagtga ttttagtttt atcttgctcc
     2221 agaagaaccg cgcctgaccc ggccagttca ttttgcgctt ttgacgctga tgaatccttg
     2281 tcagaagtcg gcaatgcgcc gacaaaacgt tccgtcaact ccgccggaaa aagcgggc
//
LOCUS       DAOSME010000188         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_49846_length_2331_cov_15.154218, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000188 DAOSME010000000
VERSION     DAOSME010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49846_length_2331_cov_15.154218"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..1117)
                     /locus_tag="PLG98_12115"
     CDS             complement(380..1117)
                     /locus_tag="PLG98_12115"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE34056.1"
                     /translation="MNSPGSFAGGVTPENILHHPPVTRNRYLVETVLLATRLVNRQNL
                     GVPRIFRALLEEGIEPPAFDEIGQTVRVTFPGQKVDKAFKALLTFLTDQQGAYLDVDD
                     LLLLHFFRRRHEAHWHDVRQAYPYDDRRLREKLASLETHLLMLEHSGSGRGMTYRLSR
                     RASAILNEGVAYDMTRRLDREAIKVRILSLLKDRPLRNADIRNFTDLGRQQVYRIMKE
                     LESDGLVYQEGHGQTALWHLKQSLSKQ"
     gene            complement(1124..2089)
                     /locus_tag="PLG98_12120"
     CDS             complement(1124..2089)
                     /locus_tag="PLG98_12120"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOE34057.1"
                     /translation="MVYTSEKIMALLPLLEEQTADDLENETLDFKECPTETRLRELAK
                     EMAVCLGNASGGTVVFGVRERVCGCVKAIVGLDFAPDLEALNATLYDSIDPKLPVQFE
                     WLTYGPRRLLLMHVHPSMPPYTTTSGKGWMRVGKDCKPLTGSLLRQLREKAGLSDPTA
                     KVLPLDNPLQAVSPSAIEILRREMKNMGSPGDLLANNDADLCGKLGFFKDGRLTVAGL
                     LVAGRDEFIADHAPNHEWKYSRLRTDTDYETPPVSGRDCILAALDKIMLLLGQHNPMT
                     TVPSGLFHAEFPQYPTIALREALLNAFAHRDYTIPGMVFIRHFRD"
     gene            complement(2128..>2331)
                     /locus_tag="PLG98_12125"
     CDS             complement(2128..>2331)
                     /locus_tag="PLG98_12125"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HOE34058.1"
                     /translation="DDGGIEIYIAARKPAGIPGENWLPINREDKDLDVILRVYLTNLG
                     KMMTWNKPKAALLKRINGDGSIF"
BASE COUNT          565 a          595 c          587 g          584 t
ORIGIN      
        1 gccccgccga tcggccggac ggcggcgcgg aaagaattat aaaaaacggc gcgcagtgtc
       61 gtcaaatagc tattaatatc atacatttaa cctctgccgg tgctccggca aagattttcc
      121 ccaggcggaa aacgagcgga aaagcccaat ctgtaaaatg aattattgat gatttaggtg
      181 tggaaataac aatcaaaatt tgactatctg tgataccctg atgtggacaa cccctgggag
      241 tgtttttatt gaaagaagcg agaatttggg gaaatatgct tctgtggcag atttcttttg
      301 gtaatgctca agcatgacct gccactctac ttggtttgca acaacagatt gattcatgat
      361 gcaaacaaga gcctttgatt cattgtttgc tcaatgattg cttcaaatgc catagagcgg
      421 tctggccatg cccctcttga tagaccagcc cgtctgattc cagctccttc atgatcctat
      481 agacctgctg acgtcccaag tctgtaaagt ttctgatgtc ggcattgcgg agaggcctat
      541 ctttaagcag agacaggatg cggaccttga ttgcttcacg atccagccta cgcgtcatgt
      601 cgtaggctac gccttcattc agaatggcgg acgcccggcg ggaaagacga tacgtcatac
      661 cccgcccgga gccgctgtgt tccagcatca ggagatgggt ttccagagag gccagttttt
      721 cacgcaaacg tcggtcatcg tatggatagg cttggcggac gtcatgccaa tgcgcttcat
      781 gacgccgcct gaaaaaatgg aggagcagaa gatcatccac atccagatag gccccctgct
      841 gatcggtcag aaaggtgagc agcgctttga atgccttatc caccttctgg ccggggaagg
      901 tcacccgcac cgtctgtcct atttcgtcaa aggccggcgg ttctatccct tcttccagaa
      961 gggcgcggaa gatacgcggg acaccaaggt tttgccggtt caccaaccgt gtggccagca
     1021 atacggtctc gaccaaatat ctgtttcttg tgaccggtgg atggtgcagg atattttccg
     1081 gtgtgacacc accggcaaaa ctgcctggac tgttgatctc gaatcaatcg cggaagtgtc
     1141 tgataaaaac catccccgga attgtatagt cccggtgggc gaaagcgttc agcaaggctt
     1201 cccggagtgc aatggtcgga tattgaggga attcggcatg aaaaagtccg gaaggaacag
     1261 tggtcattgg attatgttgc cccagaagaa gcatgatctt atccagcgcc gccaaaatgc
     1321 agtcacggcc actcaccggc ggtgtttcgt aatccgtatc tgtacgcaat cggctgtatt
     1381 tccattcatg gttgggggca tgatcggcta taaattcgtc tcgtcctgca accagcaagc
     1441 cggctacggt caggcggcca tccttgaaga aacccaattt gccgcaaagg tcggcatcat
     1501 tatttgccag cagatctccc ggagagccca tatttttcat ttcgcggcgg agtatttcaa
     1561 tcgctgatgg agagaccgcc tgcaacggat tatcaagggg cagcaccttt gccgtgggat
     1621 cgctcaagcc cgctttctcg cgtaactggc gcaacaaaga gccggttaaa ggtttgcagt
     1681 ccttaccgac tcgcatccaa cctttaccgg atgtcgtcgt ataaggtggc atggatggat
     1741 ggacgtgcat aagcagaaga cggcgtggac catacgtcag ccattcaaat tgcaccggca
     1801 gctttgggtc aatactatcg taaagggttg cgttcagcgc ctccaaatcg ggcgcgaagt
     1861 caagccccac tattgctttg acgcaaccgc agacccgctc cctgacaccg aaaacaactg
     1921 tcccgccaga ggcgttgcca agacaaactg ccatctcttt agccagttcc cgtaaccttg
     1981 tttctgtcgg gcactctttg aaatcgaggg tttcattttc gaggtcatcg gcggtttgtt
     2041 cttcaagcaa aggcagaagg gccattattt tttcagatgt atagaccatg ataagtcctt
     2101 ccatgttttt ggcatttatt tctgttatca aaaaatagaa ccatccccat ttattctttt
     2161 taacagcgct gccttcggtt tattccaggt catcatcttt ccaagattgg tgaggtaaac
     2221 acgcagaata acgtcgaggt ccttgtcctc tcggttgata ggcagccagt tttcgcccgg
     2281 gatgccggca ggtttccggg cagcaatata aatctctatc ccaccgtcat c
//
LOCUS       DAOSME010000189         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_50559_length_2306_cov_18.912039, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000189 DAOSME010000000
VERSION     DAOSME010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50559_length_2306_cov_18.912039"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..481
                     /locus_tag="PLG98_12130"
     CDS             251..481
                     /locus_tag="PLG98_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34059.1"
                     /translation="METTITAKLRIISLILLLLLILPAAARASSKPRLIFQKTFGVHP
                     AILKYSEYAYDENIVSVIHSAQCNARLWHSEE"
     gene            588..1784
                     /locus_tag="PLG98_12135"
     CDS             588..1784
                     /locus_tag="PLG98_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOE34060.1"
                     /translation="MNNQKTERILSIDILRGFCLILMVLVHFVVYWGDSAAVNTWPYF
                     FLNHVIADWGAACFLMMMGMSQVFSAQHHEGENYIPAFKRALIRGGFIFTVGICMLAL
                     AWGPAHIWQWDILTLMGFATVMLFFCRLLPSWTILLAVVGIAVCTPLLRKGIDFNAVW
                     GGGFIDVPVISSYLSGMLIDPASEYEVVWRTKEIVQGFVFTGEFPVMPWFIFPPLGFV
                     LGRRIVNGKMRNDLPFLAITGGLLALLGLGWAYASIGRDPSAIITGYIAPLSFYPDSF
                     TMICYQTGIAILIFSGMFYIYDVRKQDKNKAGFFAGIFNLASRSSLTFYFIHYLLIGW
                     PLSLIYVLTGWYYKYNLLGAYGALLAGIGAVILLLSLMKLWGNSKNKYSLEWFLDGLI
                     KRYISS"
     gene            1974..>2306
                     /locus_tag="PLG98_12140"
     CDS             1974..>2306
                     /locus_tag="PLG98_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOE34061.1"
                     /translation="MQERMPYFDHVRGILVALMIMCHTVIVYGTDRVVESPLGIFLAK
                     IMGTWPGAPVFMILMGIFFVYPNAKPASVRIARGVKLFLLGLVLNCLRLVFPYLIAKL
                     VNPDALLNF"
BASE COUNT          528 a          525 c          556 g          697 t
ORIGIN      
        1 gcgattgtaa gattgcataa gaaagagaat atcatttttg tggatttcag gaagctatac
       61 gtagcattca cgaacgggga aactgccgtt aagaaattgc acgtttcagt tcgaaagaaa
      121 tgtttctgac atctccagat ccaaccggca gttcatcttt ttgaaaacat gtggtataga
      181 ttagccatca ggcgcgttaa cgtctgaaaa attcatcacc gctggagact gaaatcttga
      241 aggaagcgca atggaaacga cgataacggc aaaacttcgc attatttcgt tgattctttt
      301 gctcttgctc attttgcccg cggccgcccg ggcctcttcc aaacccaggt tgatttttca
      361 gaaaaccttt ggcgttcatc ccgccatcct caaatattcc gaatatgcat atgatgaaaa
      421 tattgtcagc gtcatccatt ccgcgcaatg taatgcgaga ctatggcatt ccgaagaata
      481 aaattgcaaa tcgataaata actttttctt gattattctc tttgcattct ataacttaca
      541 tcattccttc actgaaacgt tgggacagta gtttagagtg atgccaaatg aataatcaaa
      601 aaaccgaacg gattctttcc atcgatattc ttcgcggctt ttgtctgatc ttaatggtgc
      661 tggttcactt tgtggtttat tggggcgact cagccgccgt gaatacctgg ccttatttct
      721 ttctcaacca tgtgatcgcc gattggggtg ccgcctgttt tctgatgatg atgggaatga
      781 gccaggtttt ttcggctcag catcacgaag gcgaaaatta cattccggcc ttcaagcgcg
      841 ccctgatccg ggggggattc attttcacgg tgggaatatg catgctggcc cttgcctggg
      901 gacccgctca catctggcaa tgggatattc tgacactgat gggatttgca accgtgatgc
      961 tctttttctg ccgcctgctg ccctcctgga cgatcctgct tgcggtcgta ggcattgctg
     1021 tttgcacacc cctgctgagg aaggggatcg atttcaatgc cgtttgggga ggcggtttta
     1081 tcgatgtccc cgtcatctca agctatctgt ccggtatgct gattgatccc gccagtgaat
     1141 atgaggtggt ctggcggaca aaagaaattg ttcagggctt cgttttcacc ggcgaattcc
     1201 cggttatgcc ctggttcatc tttccgccgc tgggtttcgt tctcgggcgc aggattgtca
     1261 acgggaagat gcggaacgat ctgccttttc tggcgataac cggcggcttg ctggccttgc
     1321 tggggctggg ttgggcctat gcctccattg gccgggatcc gtcagccatc attaccgggt
     1381 atatagcgcc gctcagtttt tatcctgact ctttcactat gatctgctat caaaccggga
     1441 ttgcgattct cattttctct ggtatgtttt acatttatga tgtccgtaaa caagataaaa
     1501 acaaggcggg gttctttgcc ggcatcttta atctggctag ccgctcctcc cttacgttct
     1561 acttcattca ttaccttctg atcgggtggc ccctgtcact gatttacgtt ctgaccggct
     1621 ggtattacaa gtataatcta ttaggcgcgt atggcgcgct gctggccggc attggggctg
     1681 tcatcttgct cttgtcgctc atgaagctat ggggaaatag caaaaacaag tacagcctgg
     1741 aatggttcct cgacgggttg ataaaacgtt atatctcctc gtgagaaaag ccgcttttga
     1801 tgttcaagtt aatttttaca aatgaggagt taaaccggcg aagccgcatc ttcgcactgg
     1861 aaaacttttg catcacttgc taaacctttt ttacaattag caggaaatca attcaatgca
     1921 tcatttaaat gttttctgaa agcgcaggaa aaaagcagaa gcattggggg tatatgcaag
     1981 aacgaatgcc gtattttgac catgtccgcg gtatactggt ggcgttaatg attatgtgcc
     2041 acacggttat tgtatacggc acagaccggg tggtggaaag ccctcttggg atttttttag
     2101 caaaaatcat gggaacctgg cccggtgcgc cggtttttat gattttaatg ggcatttttt
     2161 tcgtttatcc gaatgccaaa ccggcgtcgg tgaggattgc cagaggcgtc aagctatttt
     2221 tgctggggct tgtgctgaat tgtctgcgcc tggttttccc gtacctgatt gccaaacttg
     2281 tcaatcctga tgccctctta aatttt
//
LOCUS       DAOSME010000190         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_53094_length_2222_cov_12.585141, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000190 DAOSME010000000
VERSION     DAOSME010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53094_length_2222_cov_12.585141"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..894)
                     /locus_tag="PLG98_12145"
     CDS             complement(100..894)
                     /locus_tag="PLG98_12145"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOE34062.1"
                     /translation="MMITGKKKKSIGRSPGIFLAVFFMLWMVLPVHESWAATGYPKSR
                     GAVNDFAGVIDAENTAKMEALAQEVLQKTGTAVVVATVETIGENEDYNMYANGLYQAW
                     GIGKKGEDKGVLIFLTVKERKIRIETGYGVEGILPDGRVGEILDTYVVPFLKDGHYGK
                     GLYNAMYACSAYIAREAGVQLSGSPMPYRANAQLTQRKITVFEILFFLIAAAVLLGTR
                     RGREMLPWLLVVLMSGGRGGGGGGFGGGFGGFGGGMSGGGGAGRSF"
     gene            1045..>2222
                     /locus_tag="PLG98_12150"
     CDS             1045..>2222
                     /locus_tag="PLG98_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE34063.1"
                     /translation="MTPKIILIHPPVAKCSEPPAGIARLTSCLNANAIPYETIDANLE
                     GLLYLLKSASGNNTLSDRWTKRACKNLDRHLAAMKNIGTYQNPGRYGQAVKDINRLLN
                     VAAKPFHVALSLTDYRDRRFSPVKSADLIASAHNPQANPFYPYFKNRLTNALWSNPDY
                     IGLSLNYLSQALCTFAMIGFIKNIHPRQKIILGGSLVTSWMKLGAHLHTFGGLVDEIV
                     EGAGEQKLLEILGTQNNLIHNAPAYDDFPLDQYLSPGLIIPYSGARGCYWRQCAFCPE
                     KAEGNAYHPLEASQANAELQELTRQMNPTLIHLLDSSISPSLLQTLSQNPPGAPWYGF
                     VRVTAHLADEDFCLSLKKSGCAMLKLGIESGDQNVLDQLHKGIDLLTASAVLKNLKKA
                     G"
BASE COUNT          572 a          611 c          489 g          550 t
ORIGIN      
        1 aatatagtcc cacatggctt cgcatgtggg ataattcgac taaccaatct gactgcgctt
       61 cgcttgtcat atcggagtct cattaaaatt tgggtctcat taaaaactcc ggccggcgcc
      121 gccgccgccg ctcatgccgc ccccgaaccc tccgaaaccg ccgccgaaac cgccgccacc
      181 accgccgcgt ccgccgctca tcaatacaac cagcagccag ggaagcattt cgcggccccg
      241 ccgggtaccc agaagcactg cggcggcaat taaaaagaaa agaatttcaa aaacggttat
      301 cttcctttgc gtcaactgag catttgcccg gtaaggcatc ggcgaaccgc tcaactgaac
      361 accggcctct ctggcaatgt atgcagaaca ggcatacatt gcgttataaa gtcccttgcc
      421 gtaatgtccg tctttcagaa acggcacaac atacgtatca agaatttcgc cgacccgtcc
      481 gtccggcaga atgccttcca cgccgtaacc ggtctcaatg cggattttcc gttctttgac
      541 ggtcagaaaa attaatacgc ctttatcttc gccttttttg ccgatccccc aggcctgata
      601 aagcccgttg gcgtacatat tgtaatcttc attctcgccg atggtttcaa ccgttgccac
      661 gacaactgca gtgccggtct tttgcaaaac ttcctgagcc agcgcctcca tttttgcggt
      721 gttttccgca tcaatgacac cggcaaaatc attaaccgct ccgcgcgact tcggataccc
      781 ggtcgccgcc catgattcat ggacgggcag aaccatccaa agcatgaaga agacggcaag
      841 gaaaatcccc ggagaacggc cgatactttt ctttttcttg ccagtaatca tcatgtgtct
      901 gtcagagcgg tagtttatta ataccacgaa ctattgctta aagttttacg gctgtcaagt
      961 tcaaagcaga aagccaaaat ccttcttgcg tatgcgcaga ttacgtacta gaaaaaaaca
     1021 gtttttcaaa ggtaaggttt tattatgacg cctaaaatca ttctgattca tccgccggtt
     1081 gcaaaatgtt cggagccgcc cgcggggatc gccagactga cttcctgttt gaatgccaac
     1141 gccatcccat atgaaacgat tgatgccaat ctggaaggtc ttttgtatct gttaaagagc
     1201 gcttcgggaa acaacacctt atccgacaga tggacaaagc gcgcgtgcaa aaatctggat
     1261 cgtcatcttg ctgcaatgaa aaacatcggc acgtaccaaa atccgggtcg ctatgggcag
     1321 gccgtcaagg acatcaaccg tcttttaaat gttgcggcaa aaccgtttca tgttgccttg
     1381 tcactgaccg attaccggga cagaagattc tctccggtta agagcgccga tctgatagcg
     1441 tccgctcaca atccgcaagc caaccccttt tacccttatt ttaaaaatcg tctgacgaac
     1501 gccctttggt caaatcctga ttacataggc ttgtctttaa actacctgag tcaggcgctc
     1561 tgcacctttg ccatgatcgg ttttataaag aacattcatc cgcgacaaaa aattattctg
     1621 ggcggatcgc ttgtcacatc gtggatgaag ctgggcgcac atctccatac cttcggcggt
     1681 ctggttgacg aaattgtgga aggcgcagga gagcaaaaac tgctcgagat actgggtaca
     1741 caaaacaatt tgattcacaa cgcccctgcc tacgatgatt ttccgcttga tcaatattta
     1801 tcgcccggcc tgatcatccc ctacagcggc gcgcgcggct gttactggcg ccaatgcgct
     1861 ttctgcccgg aaaaagccga aggaaatgct tatcaccctt tggaagcctc tcaggccaac
     1921 gcagaactgc aggaattaac ccgccaaatg aatccaacgc tgattcatct tctggatagt
     1981 tctatttcgc cgtcgctctt acagactctg tcacaaaatc cgcccggggc gccctggtac
     2041 ggctttgtcc gggtcactgc acatttagct gatgaagatt tctgtttatc tttaaaaaaa
     2101 agcggctgtg ccatgctgaa gctgggtatt gaatccggcg atcaaaacgt tctggatcaa
     2161 ctccataaag gaattgattt actgacagcc tctgccgttt taaaaaattt gaaaaaagcg
     2221 gg
//
LOCUS       DAOSME010000191         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_54058_length_2192_cov_19.164717, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000191 DAOSME010000000
VERSION     DAOSME010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54058_length_2192_cov_19.164717"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..550)
                     /locus_tag="PLG98_12155"
     CDS             complement(155..550)
                     /locus_tag="PLG98_12155"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOE34064.1"
                     /translation="MRERRKEERLKILNWVSVSVVSEETQCSERIIPFNYSENISSSG
                     MKIRGSAFLPVGTRVKIDYRMNSGSDKTITVTGKIVWIRMIIENKYYEAGVEFAQLSD
                     ETAGKLKDYIQSKIDKKDKAPFKVDLDHK"
     gene            complement(810..2102)
                     /locus_tag="PLG98_12160"
     CDS             complement(810..2102)
                     /locus_tag="PLG98_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE34065.1"
                     /translation="MGKTKQGKPNKLQKTKTTGKKGKTVKGAAPAGRKNPVVPLTADE
                     IYRQLFNLSPNAIAVFKPEDGTICNVNDFLCQGSGYSREEIIGRTSQDLQLFSQDIRE
                     KALACIAKDGCFRGLEVQHRLKDGSMVTCLDSGDLVKIGDEYYVIVVAQDITHIKNIE
                     EALHNEHNLNKAIINNLPGLFYVIDEYFRFQLWNDNYTRITGYTSDDLRRMSALNLAP
                     PDKRSSTVEKIKSGFDIGESSGEGEIVCKDGSFKTFLFNSRIINYKGKRCMMGTAMDI
                     TDKKKSEEDLKRFAESLEDANIALRVLINHSNNDKKMLEDKLQTNINDLVMPYIDKLK
                     NMHLDERSRKFISILETNLGSVLSPFMTDLREYHKKMTPQEMQIIDLIRKGKITKEIA
                     SILNVSVNTIATHRNNIRKKLNLRKTKSNLRSYVRSIK"
BASE COUNT          562 a          543 c          438 g          649 t
ORIGIN      
        1 cacgccggaa tgacagtggt acggtacacg cgggaatgac agtggtacgg agcatgaggg
       61 aaggacagcg gtactttgaa ccatgaacca taaacccgtt ttttacttaa aacgtaaaac
      121 gtaaaactga aaactatttt gcatgaacca taaactattt atgatccaag tcgactttga
      181 aaggcgcttt atctttttta tcgattttac tctgaatata atccttcaac tttccggcgg
      241 tctcatccga aagttgcgca aattccaccc ccgcctcgta atacttattt tcaataatca
      301 ttcttatcca gacaattttc ccggtaacgg taatcgtttt atccgagccc gaattcatgc
      361 ggtaatcaat tttgacccgc gtgccgaccg gaaggaacgc acttccgcgg attttcatac
      421 ccgacgacga aatattttcg ctgtaattga agggaattat tctttcggaa cattgcgttt
      481 cttcggaaac caccgacacg gaaacccaat ttaaaatttt caaccgctcc tcttttcttc
      541 tttcccgcat tccaacgact ccctgtacga caaattggat aaaatgtaat gataaaggcc
      601 ctgcggaagg ttggttcacg aacacaaaaa tggaggagat atcttctcct gatttccccc
      661 aggaaagggc catccgcagg gcacaatccc ctgttccatt cacgatcaca tgaagaaaac
      721 ttttaaagca atacggcaaa cagaataata tttcaacaag gcagacacat ggaattttga
      781 tgataaaacg tgtacagaaa aatacacatt tatttgatgc ttctcacata agaccggaga
      841 ttgcttttgg ttttcctgag gttgagcttc ttgcggatgt tattgcggtg cgtcgcgatc
      901 gtgttcaccg agacgttaag aatggaggca atttcttttg taatttttcc tttgcggatc
      961 agatcgataa tctgcatttc ctgcggcgtc atttttttat gatactcccg cagatccgtc
     1021 ataaaaggcg aaagcacgct gccgagattg gtttccagaa tgctgataaa ttttctgctg
     1081 cgttcatcca gatgcatatt cttgagcttg tcgatataag gcatcaccag atcgttgatg
     1141 ttggtttgca gcttgtcctc cagcatcttc ttgtcgttgt tactgtgatt aatcagcacg
     1201 cgcagcgcga tattggcatc ttccagactt tcggcaaagc gcttcagatc ctcttcggat
     1261 ttctttttat ccgtgatatc catcgccgtg cccatcatac agcgcttgcc tttataattg
     1321 atgattctcg aattaaacaa aaatgtttta aagctgccgt ccttgcagac aatctcgcct
     1381 tcgccggaac tttctcctat atcaaatccc gattttatct tttccacggt actgctgcgt
     1441 ttatcgggcg gtgcaagatt cagcgccgac atccggcgca aatcgtcgga tgtgtatccg
     1501 gtgatgcggg tataattatc attccagagc tggaacctga aatattcatc aatcacataa
     1561 aacaaccccg gcagattatt aataatcgcc ttgtttaaat tgtgttcatt atgcagagct
     1621 tcttcaatat tcttaatgtg cgtaatatcc tgcgccacca caatgacata atattcatcg
     1681 ccgattttga ccagatcgcc cgaatcaagg caagtgacca ttgaaccgtc ctttaaccgg
     1741 tgctggactt ccaggccccg gaaacaaccg tccttggcaa tacacgccag cgctttttcg
     1801 cggatgtcct gagaaaataa ctgcaaatcc tgactggtcc tgccgataat ctcttcccgt
     1861 gaatagcccg atccctggca caaaaaatca ttgacattgc agatcgttcc gtcttccggt
     1921 ttaaaaaccg caatcgcatt cggcgacaga ttgaagagct ggcgataaat ctcatcggcg
     1981 gtgagcggca ccaccggatt ttttcgaccc gcaggcgctg cccctttaac cgttttccct
     2041 tttttgccgg ttgttttggt tttctgcaac ttattcggtt tcccctgttt tgtttttccc
     2101 ataacgtctt ctcctaaccc cttgtgtttt caaatagtgt catccccgaa tgactccctg
     2161 tttgtcatac cggcgcaggc cggtatccag aa
//
LOCUS       DAOSME010000192         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_55048_length_2161_cov_22.751662, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000192 DAOSME010000000
VERSION     DAOSME010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55048_length_2161_cov_22.751662"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..583)
                     /locus_tag="PLG98_12165"
     CDS             complement(80..583)
                     /locus_tag="PLG98_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOE34066.1"
                     /translation="MGKIIRSNTQKVYDKSPSSNGKRRIQVRQSGIHGKGVFAVHPID
                     AGETLLEYKGEIITWTKALARHPHDKSQPHHTFYFHLDDHHVIDAKYNGNSAKWINHS
                     CQPNLEAEQKGNRVFLKALRHIKAGEELSYDYSLVVDSRKTPRLKKEYACYCGAANCR
                     GTMLDCK"
     gene            complement(834..1166)
                     /locus_tag="PLG98_12170"
     CDS             complement(834..1166)
                     /locus_tag="PLG98_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE34067.1"
                     /translation="MFYKANQNGFKEVLPGIKLKTLVYGEKSLFSEFRMTAGSILPKH
                     AHFHEQTGYLTEGKIRLTIGNETFDVEIGDSWCIPGNVEHSAEILKDAVAIEVFTPVR
                     EDYLPGNP"
     gene            complement(1193..1981)
                     /locus_tag="PLG98_12175"
     CDS             complement(1193..1981)
                     /locus_tag="PLG98_12175"
                     /inference="COORDINATES: protein motif:HMM:NF038213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE34068.1"
                     /translation="MAAQNKGRYKSNKPLTGKTAIVTGASSGIGKATAMTLAQAGAAV
                     VIQARRKDRLVKLASDIEAKGGKALAISGDASLTSDTDRLLEKTLEWKPGGRKYDIVV
                     VNAGRGLAGGMLSSDHYQWEEVYHTNVLGAAYLMRSAAKYFVERKKGDIVAIGSVVGR
                     NISPFSAFYGSSKFAVGAIVEGLRQEVCSHDVRVSLVMPGIVKSEFQKVAGYNEDNFY
                     KGIAHMGKLLDPQAIADGIYWLLTQPRNVNISEIMIRPTGQNYP"
BASE COUNT          488 a          522 c          519 g          632 t
ORIGIN      
        1 tgttttttta aatacaccct gcctggtgtt ttatgcaata agaaaatccg tataggaggc
       61 gtcgggatca aaaatgccct tatttacaat cgagcatggt gccccggcag ttggcggcgc
      121 cgcagtagca ggcgtattct tttttaagcc ggggtgtttt acgggagtcg acgaccaggc
      181 tgtaatcata agagagttct tctccggctt ttatatgccg taacgctttg aggaaaacgc
      241 gattgccttt ctgttcggct tccagattag gctggcacga gtgattaatc catttcgcgg
      301 aattgccgtt atacttggcg tcgatgacgt ggtgatcgtc caggtgaaaa taaaaggtgt
      361 gatggggttg acttttgtcg tgcgggtgac gtgccagagc tttcgtccac gtgatgattt
      421 cgcctttgta ttccagaagc gtttcaccgg catcaatggg gtgaacggca aaaacccctt
      481 tgccgtggat gccggattgg cgcacctgaa tacggcgttt gccgtttgat gacggcgatt
      541 tgtcataaac tttctgtgtg tttgatctga ttatttttcc catcttcata aagcgcttgt
      601 tcatcgataa gttttgtggg cgtgtataca aaaggtgcgt ccggtagtca agatgaaatt
      661 tgcgggacag cggatcattt acgatatcag cctttgtgtc tagatatttt ttgagcggaa
      721 aaaattcttg atgccgcata ttgcagtatg gcaaggacac tgtccggaaa taccttcgcc
      781 gaaacgcttc cccaacttca gtgtcacact taccgatttc gatggccttc tgtctatgga
      841 ttgcccggaa gatagtcctc gcgaacgggc gtgaagactt caatggcaac ggcgtctttc
      901 aatatttcgg cgctgtgttc cacattgccc gggatacacc aactatcccc tatttcaacg
      961 tcaaacgttt cgttgccgat ggtgagccgg attttacctt ccgttaaata tcccgtctgc
     1021 tcatgaaaat gcgcgtgttt cggaagtatc gagcctgcgg tcattctgaa ttcagaaaag
     1081 agagactttt ctccgtaaac aagagttttg agctttatac ccggcaatac ttctttaaat
     1141 ccgttttgat tcgccttgta aaacaatcga ttctcctttt tgacgttaat cattatgggt
     1201 aattctggcc cgtgggcctg atcatgattt cgctgatatt gacgttcctc ggctgtgtga
     1261 gcagccagta aatgccgtcg gctatggctt gcggatccag taatttcccc atatgcgcga
     1321 tccctttata gaagttatct tcgttgtaac cagccacttt ttgaaattcg ctttttacga
     1381 ttcccggcat gaccagagat acgcgaacat catgcgagca aacttcctga cgcaaacctt
     1441 ctacgattgc accgacggca aatttgctgg agccgtaaaa cgcgctgaag ggagaaatgt
     1501 ttcttcccac aacggatcct atggcgacga tatcgccttt ctttcgttcg acgaaatatt
     1561 tggccgcgga gcgcatcaaa taagcagcgc cgagcacatt ggtatgataa acctcttccc
     1621 attgatagtg atcgctggac agcatgccgc ctgccaagcc gcgaccggca ttaaccacta
     1681 caatatcata cttacgaccg cctggtttcc attctaaagt tttctccagg agcctgtctg
     1741 tatcggacgt caggctggca tcgcccgata tggctaaggc cttgccgccc tttgcctcga
     1801 tatccgaagc cagtttgacg agcctgtctt tacgccgtgc ctgaataacg accgccgctc
     1861 ctgcctgcgc cagcgtcatc gccgttgctt tcccgatgcc ggaactggcg ccggtgacaa
     1921 ttgcggtttt tccggtcaga ggtttgttgg atttatacct gcctttattt tgcgccgcca
     1981 tatttacctc cttttgttct cttatcgggg aaaaagaaat gaatgtcctt ttggatatgg
     2041 ttcagccaaa ttttacgctg cttttccgta ctggtaacaa tgacattgaa catccggcga
     2101 tgaaaattgg tgatgccgca gagaccgaag atgcaattct tccagatggt ttccaacgga
     2161 t
//
LOCUS       DAOSME010000193         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_55049_length_2161_cov_20.830484, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000193 DAOSME010000000
VERSION     DAOSME010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55049_length_2161_cov_20.830484"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLG98_12180"
     CDS             <1..186
                     /locus_tag="PLG98_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34069.1"
                     /translation="GRGHERGLPVPPLRRRGMILSCGEIRYKSMMETVFQQFNIWAQP
                     NDTLRRLCAINQDAVYN"
     gene            204..449
                     /locus_tag="PLG98_12185"
     CDS             204..449
                     /locus_tag="PLG98_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34070.1"
                     /translation="MARWHYKTNIIDLDKVVPPEAIECDLTGSCLVSDLQGKGLGHFQ
                     GMLDKEGESEWELVQCQYHGGKLLCIWKKEVKEAFAS"
     gene            557..1438
                     /locus_tag="PLG98_12190"
     CDS             557..1438
                     /locus_tag="PLG98_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOE34071.1"
                     /translation="MAFNAPPKMVGLVGNAGVYKSKLSIFQFLLRGFMAGAYIAIGGA
                     LATVCSTGAAAYLGPGVAKLITGAVFPVGLIAIVLTGMELFTGDCMLVPMAAMMKRCT
                     WGAVWKNWLWVYIGNVIGSFFYAYVMAVGPFVDGTASGAMAVNVFGLNAVNIAVAKIL
                     TYKAVGSAGIWSCFMKAIGCNFLVNIAILLAITADDVIGKFFAIWFPIMAFVSSGFEH
                     CVANMYFLPAGKFLTDWHPEIIAKVGGLLNANNGLSWSDVWFWNIIPVTIGNIVGGFL
                     FIGVAYFLMFRKEVPKD"
     gene            1451..1945
                     /locus_tag="PLG98_12195"
     CDS             1451..1945
                     /locus_tag="PLG98_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34072.1"
                     /translation="MSALAWIAFIVCSIGVWLHYLEVKDSRFLMYAIPGLILLIVIPM
                     TLAWMSRRTFTRAERNYGQGARAYKIGRINTSLSLLGEVVIIRGEVQKISFKWLNRPH
                     YHVKDDTGTIRVIMFTAPAENIEQGATVEALGTVVRNVFDRRRQAISAVSIKKQISRT
                     KKTE"
     gene            complement(1948..>2161)
                     /locus_tag="PLG98_12200"
     CDS             complement(1948..>2161)
                     /locus_tag="PLG98_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34073.1"
                     /translation="LFTMPSSIAPALCYGDDLKKAVEAATIMPSSIIISVFPAARCFA
                     KTGTTPNVMKPIKKTSSRVSIITPCS"
BASE COUNT          541 a          457 c          580 g          583 t
ORIGIN      
        1 ggaagaggcc atgaaagagg cctgccggtg cctccgctgc gacgcagagg tatgatttta
       61 tcttgcggag aaatacgcta taaatcgatg atggaaaccg tttttcagca gttcaatatt
      121 tgggcgcaac ctaacgacac attgcgtcga ttatgcgcaa tcaatcaaga cgcggtgtat
      181 aattaagtgt ggggaggatg atgatggcaa gatggcatta caaaacgaat attattgatc
      241 tggacaaggt tgtacccccg gaagccattg aatgtgatct taccggaagc tgcctggtct
      301 ctgacctgca gggaaagggt cttggtcact ttcaaggcat gctggacaaa gaaggcgaga
      361 gcgaatggga gttggttcag tgtcaatatc atggcggcaa actgctttgc atttggaaaa
      421 aggaagttaa agaggctttt gccagttaac cctgcaaaaa cctttgtttc tttcttattt
      481 tattaagtta cggtgtcatc aaaaatcaag atagggacac cagtgattta ttttactaaa
      541 aaatggagga atcgagatgg cttttaatgc accacccaaa atggtaggtc ttgtcggcaa
      601 cgccggagtt tacaagtcga aactttcaat ttttcaattt ctgcttagag gctttatggc
      661 gggggcctat attgccatag gaggggcgct ggcgacggta tgttccacgg gagcggcggc
      721 atatcttggc cccggggtag caaagctgat tacgggagcg gtgttccccg tcggtttgat
      781 cgctattgtg ctgacgggca tggaactctt cacgggggat tgcatgctgg ttcccatggc
      841 cgccatgatg aagcggtgca cgtggggcgc cgtatggaaa aactggttat gggtttacat
      901 agggaacgtt atcggttcct ttttttatgc ctatgtgatg gccgtcggac cctttgttga
      961 cggcacagca agcggcgcca tggcggtcaa cgtctttggc ctgaatgccg tcaatattgc
     1021 cgtggcgaaa attctcacct acaaagcggt gggttcggcg gggatatggt cttgtttcat
     1081 gaaggcaatt ggttgtaact tcctcgtcaa catcgcaatt cttttagcta ttaccgccga
     1141 tgacgtgatc ggcaaattct tcgccatctg gtttcccatc atggcatttg tttccagcgg
     1201 cttcgaacac tgcgtggcca acatgtactt cttgcccgcc ggtaagtttc tgaccgattg
     1261 gcatccggag attatcgcga aagtgggcgg attgttgaac gccaataatg gattgagctg
     1321 gagtgatgtc tggttctgga acatcatccc cgttaccatc gggaatatcg tgggtggttt
     1381 cctcttcatc ggggtcgctt atttcctgat gttccgaaaa gaggtaccta aagattaaaa
     1441 aggcgacgct ttgtcagcat tagcttggat agcctttatc gtttgttcca tcggggtgtg
     1501 gctacattat cttgaggtca aagattcccg atttttgatg tatgccatcc ccgggttaat
     1561 tctgctcata gttatcccga tgacattggc atggatgagc cgcaggacat ttacccgggc
     1621 ggaacgtaac tacggtcaag gggccagagc atacaagatc ggcagaataa atacgtcttt
     1681 gtccttattg ggggaagtcg tcatcattcg cggcgaggtg caaaagatct ccttcaagtg
     1741 gctcaataga cctcactatc atgtaaaaga cgatacagga acaataaggg taatcatgtt
     1801 taccgcgcca gcggaaaata tcgagcaggg agcgaccgta gaggcccttg gcaccgtagt
     1861 caggaatgtt tttgatcgcc gccgccaggc catatcggct gtcagtataa aaaaacagat
     1921 ctctcggacc aaaaaaacag aataatctta actacagggg gtaatgatgg aaacacgtga
     1981 actggtcttt ttaattggct tcattacatt gggtgtggtg cctgttttcg caaagcatct
     2041 tgccgcggga aacacggaaa ttattatgct gctaggcatg attgttgccg cttcaacggc
     2101 tttttttaaa tcatcaccat agcataaagc gggagcgatg cttgacggca tcgtaaaaag
     2161 t
//
LOCUS       DAOSME010000194         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_56575_length_2119_cov_15.230136, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000194 DAOSME010000000
VERSION     DAOSME010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56575_length_2119_cov_15.230136"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLG98_12205"
     CDS             complement(<1..823)
                     /locus_tag="PLG98_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE34074.1"
                     /translation="MKVIRLKAIIRKEFYHLIRDYRSLYLAFAIPLLLIILFGYALSL
                     DVDNVETVFIDYDRSDLSRDFIRRLDASSYFHVSGVLPSTNDAVHYLDQDRARLAIVI
                     PPDFKKSIGADRATPLQIIIDGSDPTFGNIMRGYITAFVEQYNQKLLIDFLNRQGQEK
                     INPPADGRIRIWFNEDMESRNFIIPGIIAVIIMIVGALLTSLVIAREYENGTMETIKS
                     LPVRAGELLLGKALPYFLIGLADVLIAILLSQVLFGIVMKGNFWLMILSSSLYLSV"
     gene            complement(820..1575)
                     /locus_tag="PLG98_12210"
     CDS             complement(820..1575)
                     /locus_tag="PLG98_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE34075.1"
                     /translation="MTPSNSDSISVTHLEKRFGNFVAVNKISFSVKKGEIFGFLGSNG
                     SGKSTTIRMLCGIITPTSGGGTVAGHDIVTEAEEVKLSIGYMSQKFSLYEDLTPFENL
                     RFYLGVYNVPSSQWRERIDWVLNMTRLQDARDRKTRELPPGWRQRLALGCSLLHKPDI
                     LFLDEPTSGVDPVTRRHFWRFIRTLAEGGMTIFVTTHYMDEARFCERIVMINEGNIVA
                     AGTPADIVAGTCPDLTDADLTDAFIKIMKRDKM"
     gene            complement(1556..>2119)
                     /locus_tag="PLG98_12215"
     CDS             complement(1556..>2119)
                     /locus_tag="PLG98_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE34076.1"
                     /translation="DGLHVVAEKQKIKNIIGYMPQRFGLYQDLTVEENMDFFMDIFAV
                     PRSQRKIRKDKYLGFSNLLPFTDRMAGNLSGGMKQKLGLACVLVHEPQLLILDEPTNG
                     VDPVSRSEFWEILENMQKEGMTIIVSTAYLDEGEKCNRLGLMHDSVLLDLAAPSVIRG
                     GFPTLEEAIIERIKQADGALAHDTFQL"
BASE COUNT          514 a          548 c          558 g          499 t
ORIGIN      
        1 cgacgcttaa ataaagagag gaggagagga tcatcagcca gaaatttcct ttcataacga
       61 ttccgaacaa cacctggctt aacagaatag caatcagcac gtccgccagt ccgatgagaa
      121 aataaggcag cgctttgccc agcagcaatt cgccggcccg gaccggcagc gatttaatgg
      181 tttccatcgt gccgttttca tattcccgcg caatgaccag ggacgtcagc agcgcgccga
      241 cgatcatgat gatgaccgca ataatgcccg gaatgatgaa gttgcggctt tccatgtctt
      301 cattgaacca gatgcggatt cgtccgtccg caggcggatt gatcttttcc tgtccctgcc
      361 gattgaggaa atcaatcaaa agcttctggt tgtattgttc aacaaaggcg gtgatatagc
      421 cgcgcatgat gttgccgaag gtcggatcgc tgccgtcgat aatgatctgc aacggcgtcg
      481 cccggtcggc gccgatgctt tttttgaaat ccggcgggat gacaatggcc agccgggcac
      541 ggtcctgatc cagataatga accgcgtcat ttgttgacgg tagtacgccg gaaacgtgaa
      601 aataggaaga ggcatcgagc ctgcggatga aatcccggct gaggtcgctg cggtcgtaat
      661 caatgaaaac tgtttccaca ttgtccacat cgaggctcag cgcatagccg aacagaataa
      721 tcagtaaaag cgggatggca aaggccagat agaggcttcg ataatcacga atcaggtgat
      781 aaaattcctt gcgaataatg gctttgagtc tgatgacctt cacatcttat ctctcttcat
      841 gatttttata aacgcgtcgg tcaggtcggc gtcggtcagg tcgggacagg ttccggccac
      901 aatgtcggct ggtgttcccg ccgcgacgat attgccttcg ttgatcatca cgattctttc
      961 gcagaaacgc gcttcatcca tgtaatgcgt tgtgacaaag atcgtcatgc cgccttcggc
     1021 cagtgtccgg atgaaacgcc agaaatgcct gcgggtgacg ggatcaacgc ccgacgtcgg
     1081 ttcgtccaga aacaaaatat ccggtttatg taaaagcgag cagcccagcg ccagtcgctg
     1141 acgccagccc ggcggaagct cccgggtttt ccggtcgcgt gcgtcctgca ggcgggtcat
     1201 gttcaatacc caatcaatgc gctcgcgcca ttgtgacgag ggaacattgt agacgcccag
     1261 gtaaaagcgc agattttcga aaggcgtcaa atcttcataa agagaaaatt tctgcgacat
     1321 ataaccgatg gacagtttga cctcttctgc ttcggtgacg atgtcatgac cggcgacggt
     1381 gccgccgccg gatgtcggtg tgatgatgcc gcaaagcatc cggatggtcg tggatttgcc
     1441 ggaaccgttc gagccgagga atccgaagat ctcgcccttt ttgacggaaa aagaaatttt
     1501 gttgacggca acaaaattgc cgaatctttt ttccagatgg gtgacagaga tggaatcaga
     1561 gttggaaggt gtcatgcgcc agcgctccgt ccgcttgttt gattcgttcg ataatcgcct
     1621 cctccagcgt gggaaatccg ccgcgaatga ccgagggcgc cgccaggtcc aaaagaaccg
     1681 aatcatgcat taaccccagt ctgttgcact tttctccctc gtcgaggtag gcggtggaaa
     1741 caatgatggt catgccttct ttctgcatat tttccagaat ctcccagaat tcgctgcgtg
     1801 ataccggatc aacgccgttg gtcggctcat ccaggatcag caactgcggc tcatgcacca
     1861 gcacgcaggc cagtcccagc ttctgtttca tgccgccgga aaggttgccc gccatgcggt
     1921 ccgtaaacgg caacagattg gaaaacccca gatatttgtc cttgcgtatt ttcctctgac
     1981 tgcgcggcac ggcaaagatg tccatgaaaa aatccatatt ttcttcgacg gtcaaatcct
     2041 ggtagagccc gaaccgctgg ggcatataac cgataatatt ttttattttt tgtttttcag
     2101 caacgacatg gagaccgtc
//
LOCUS       DAOSME010000195         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_56760_length_2114_cov_15.376882, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000195 DAOSME010000000
VERSION     DAOSME010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56760_length_2114_cov_15.376882"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PLG98_12220"
     CDS             complement(<1..1151)
                     /locus_tag="PLG98_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HOE34077.1"
                     /translation="MREGVEPLVNDGFAGDAAKRCLSYIQSQREKGKNVVGIYCGYAP
                     MEVIRAADIVPAVLCAFADKTIAAGETVLPANLCPLIKSSYGFIRTDTCPFYALSEAV
                     IAETTCDGKKKMFELISDIKPTHVMDLPQLPDQKEAEDNWTTMIRKLAGFLETTFKTK
                     ISDDRVEQEIKNTNLKNKLLNQIFDFAAFPSVPLTWQEVYDLTYFGQVATTEDMLSML
                     EASLAKLQKRVADGVYHGKKDSPRVLVSGCPVGGDATKVFNIIEEAGGVIVYLDACTG
                     MKAYAGHFEEGAPDPYRSVSKRYLDVPCSCMTPNDRRLSELDLIIDKFKPDVIIDVVL
                     HACHSYNIESSKVKTHVETRHKKPFLKIETDYSQGDVEQIRTRVEAVLET"
     gene            1408..>2114
                     /locus_tag="PLG98_12225"
     CDS             1408..>2114
                     /locus_tag="PLG98_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOE34078.1"
                     /translation="MGDYTKMWTELGLDLKGHDALLSVLGGAYTNIFLSQKNRPEGMR
                     YFDFVMSEVHGLRIQELMDAKAQGRKVIGSYCVFVPEELILAVDGVSVGLCAGAEFNF
                     EGAETMLPRNTCALIKSAFGFKLGQVCPYLEASDVVVGENTCDGKKKSYEMLAPLVKD
                     LYVMDMPQMKSESGRTLLKEEYRKLIDKLEKVSGKKVTVESLKKGIDIVNAKRAAVRR
                     LTELRAADPAPISGLDAL"
BASE COUNT          527 a          555 c          512 g          520 t
ORIGIN      
        1 gtttcgagca ccgcctccac gcgagtacgg atttgctcca catcgccctg cgaataatcc
       61 gtttcaattt tcaaaaacgg ctttttatgt ctggtttcca cgtgcgtctt caccttggat
      121 gattcgatat tatacgaatg acatgcgtgc aacacaacgt caataataac atccggtttg
      181 aacttgtcga tgataagatc gagttccgaa agcctccggt cattaggcgt catacaggaa
      241 cagggcacgt cgagataccg tttggaaacc gaccggtagg gatccggcgc gccttcctcg
      301 aaatgtcccg cataggcctt catgccagta caggcatcca gataaacgat cactccgcct
      361 gcttcttcaa tgatgttaaa aaccttggtg gcatcgccgc caaccgggca ccccgaaacg
      421 agcacacgag gtgaatcttt tttcccgtga taaacgccgt cggcaacccg tttttgaagt
      481 ttggccagtg aggcttctaa catcgacagc atatcttcag tggtggctac ctgcccgaaa
      541 tacgtcagat cgtaaacttc ctgccaggtc aaaggcacag acggaaaagc cgcaaaatca
      601 aaaatctgat tgagcaactt gtttttgaga tttgtatttt taatttcctg ctcgacccgg
      661 tcgtccgata ttttcgtctt gaatgtcgtc tccagaaaac cagcgagctt tctgatcatc
      721 gtcgtccagt tgtcctcggc ttccttctga tccgggagct gcggcagatc catcacatgc
      781 gtcggtttga tgtccgaaat cagctcgaac atttttttct tgccgtcgca ggtcgtctcg
      841 gcaatgaccg cctcagaaag ggcataaaac ggacaggtgt ccgtcctgat aaagccgtag
      901 ctcgatttga tgagcggaca gagattggcc ggcagcaccg tctcgcccgc ggcaatggtt
      961 ttatcggcaa aagcgcacaa aacggcaggc acaatgtccg cggcgcgtat tacttccatc
     1021 ggcgcgtacc cgcaatagat ccccactaca tttttccctt tttcacgctg ggattggata
     1081 taactcaagc accgtttcgc cgcatccccg gcaaaaccgt cattaaccag cggctcaact
     1141 ccttctctca tactgctgcc tcccgtaatg tcttgccgat tagctatatg atccaccgtc
     1201 atatatccaa tagataatag ttataatccc cgacgcatta tttcttttat tcattggcgc
     1261 aatccaaatc acaatgatac gaaggcgaag cttgtgcaac ccgcgcaacg aacatccgga
     1321 cttcttcact tttctgacgt ttgcggttaa cggtcaaaca tttcatcgat ccggataaaa
     1381 gactaaaata acggttggag gtttattatg ggggattata caaagatgtg gacggagctg
     1441 ggacttgacc tgaaaggtca tgatgcgctt ctttccgttc ttggcggcgc ttacacaaac
     1501 atttttcttt cacaaaaaaa tcgtcccgag ggcatgagat attttgattt tgtcatgagt
     1561 gaagtgcacg ggctgcgcat tcaggaactg atggatgcca aggcccaagg cagaaaagtg
     1621 atcggttcct attgcgtttt tgttccggag gaattgatcc tggccgttga cggcgtatcc
     1681 gtgggtctgt gcgccggagc ggaattcaat tttgaaggcg cggaaacgat gcttccccga
     1741 aacacctgcg cgctcatcaa atccgccttc gggtttaaac tgggccaggt ctgtccgtac
     1801 ctggaagcca gcgatgttgt cgtcggtgaa aacacctgcg acggcaagaa gaaatcctat
     1861 gaaatgctcg cgccgctggt caaggatctt tacgtgatgg acatgcccca gatgaaatcg
     1921 gagtcgggca ggacgctgct caaagaggaa taccgaaaac tcatcgacaa actggaaaag
     1981 gtcagcggga aaaaagtaac cgtggaatcc ctgaaaaagg gcattgacat cgtcaacgcg
     2041 aaacgcgccg ccgtccggag actgaccgaa ctgcgcgccg ctgatccggc gccgatatcc
     2101 ggactggatg ccct
//
LOCUS       DAOSME010000196         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_57632_length_2091_cov_18.731336, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000196 DAOSME010000000
VERSION     DAOSME010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57632_length_2091_cov_18.731336"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..953
                     /locus_tag="PLG98_12230"
     CDS             369..953
                     /locus_tag="PLG98_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34079.1"
                     /translation="MHKIFFRKMNEKWKTFFFLALIFAISSCYYYPQEQMVTRPSPDE
                     NIRAITQIYFYPNRNQSADLQSRDHYACYTWAMEQTGFDPSVSSIVPEQRVRVVPMPP
                     PGQDTIAMSIAGAVLGALIAGPRHAGGGALIGAAGGAMAGMVSDASRVEAARQMEEAY
                     NNRNQGRDFRREKKALQFRRAMSACMEGRGYTVK"
     gene            978..1724
                     /locus_tag="PLG98_12235"
     CDS             978..1724
                     /locus_tag="PLG98_12235"
                     /inference="COORDINATES: protein motif:HMM:NF040452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HOE34080.1"
                     /translation="MISVKFNFKKCFVVVLSLFMLTFVSELVFTPASQAQPSRKSSVR
                     TVKKGSTVHHRKQFFDARHSHNRAYPVRGHYFRSLPRDHRVIRHGSSRYYSTSHGVWY
                     RPYRGLYEVIAPPIGIFVPFLPLFYTTVRVQGTPYYYANGTYYTQTPGGYVVVHPPQG
                     EVMENPPVTDDEDEGFMDDKLFIYPSKGQSEKQRDLDRYECHQWALEQADYDPTQISS
                     GRSADEVMQGRVEYRRAIAACLESRGYAVK"
     gene            1806..>2091
                     /locus_tag="PLG98_12240"
     CDS             1806..>2091
                     /locus_tag="PLG98_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34081.1"
                     /translation="MERQKILQQMIDLNKIFFDSWFSMMAMFQHQTEEILKPFVDCMP
                     GMDGRMKKFIRHWSEECKKNRETFKRDIDAGYAKAEAFFDYHAILKFQEQN"
BASE COUNT          563 a          467 c          536 g          525 t
ORIGIN      
        1 acctttgagg gaaagaatca gaaccctcgc ttgtttgatc aggaaggatt tgccggcaga
       61 aagctgggaa cggcgaattc tccagatgtc atgggcgatg aaattggcga gctgtcggat
      121 ctttttcata atgtctttca tgatggcgct ccgcgaatcc ggctgaaaga taattttcag
      181 acaggaagat aagaataaag ataacagata gatatgttaa tcgcaaaaga tttttcatcc
      241 gtgttgaggc agcataagcc caaagggctc attttgatta atgattttaa ttgatttaat
      301 taaaaattga aaagtgctat tattgtaaaa cagccgtaaa aaaattaaca ggaccaggga
      361 gggaaaacat gcataaaata tttttcagaa agatgaatga gaaatggaaa acattttttt
      421 tccttgccct catttttgca atcagttcct gttattatta tccacaggag caaatggtga
      481 cccggccgtc tcctgatgaa aatatcaggg ctattacgca aatttatttt tatcccaacc
      541 gtaatcagtc ggcggatttg caatcgcgcg atcactatgc ctgctatacc tgggccatgg
      601 aacaaaccgg ctttgatccc agcgtgtcct ccattgtgcc ggaacagcgt gtccgcgttg
      661 ttcccatgcc gccgccggga caggatacca tagccatgtc gattgccgga gcggttctgg
      721 gtgcgttaat tgccggaccg agacacgccg gcggcggggc attgatcgga gcggccggtg
      781 gcgccatggc cggaatggtt tctgacgctt cccgggttga agcagccagg caaatggaag
      841 aggcgtacaa caaccgtaat cagggacgcg atttccgcag ggagaaaaaa gccctgcagt
      901 ttcggcgggc catgtccgcc tgcatggaag gtcgcggata tacggtaaaa tagttttgca
      961 tgcataagga ggttacgatg atttcagtaa aattcaactt taaaaagtgt tttgtcgttg
     1021 tcctttcctt attcatgttg acttttgtga gcgaattggt tttcacccct gcatcacagg
     1081 cgcagccttc caggaaatcg tcggtacgta ccgtgaagaa aggatcgaca gtacaccatc
     1141 ggaagcaatt ctttgatgcg cgtcacagcc ataatcgcgc gtatcccgtc cgcggtcatt
     1201 atttcagatc gcttccccgc gatcaccggg tgatccgtca cggcagctct cgttattact
     1261 ccacgtctca cggcgtctgg tacagaccgt accgcggact ctatgaagtg atcgctccgc
     1321 cgatcggaat ttttgtgccg tttttgcctc ttttttacac caccgtccgg gtgcagggca
     1381 cgccgtacta ctatgcgaat ggaacctatt acacgcaaac cccgggaggc tacgtggttg
     1441 ttcatccgcc tcagggagag gtcatggaaa atcctccggt aacggacgat gaagatgaag
     1501 gtttcatgga tgacaaactc tttatctatc ccagcaaggg gcagagcgaa aaacagcgtg
     1561 atctggaccg gtatgagtgc catcagtggg cattggagca ggccgactat gatccgactc
     1621 aaatttcttc aggtcgttct gccgatgagg tgatgcaggg aagagtagaa tacagacgcg
     1681 ccattgcggc ctgtctggaa agccgcgggt atgccgtaaa ataatcgctt aaagcggtcg
     1741 aaaataaaat tccccataat cccctttttc atgaaagggg cctgtcaggc ctggaggaat
     1801 taacgatgga aagacaaaaa attctgcagc agatgatcga tctgaataaa atattttttg
     1861 acagttggtt ctccatgatg gcaatgtttc agcaccagac ggaagagatc ctgaaacctt
     1921 ttgtggattg tatgccggga atggatggcc ggatgaagaa attcatccgg cattggagcg
     1981 aggaatgtaa aaagaatcgt gaaacgttca aacgtgatat cgatgccggc tacgcaaagg
     2041 cggaagcttt tttcgactat catgccattc tcaagtttca ggaacaaaac g
//
LOCUS       DAOSME010000197         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_58690_length_2063_cov_23.732570, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000197 DAOSME010000000
VERSION     DAOSME010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58690_length_2063_cov_23.732570"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLG98_12245"
     CDS             complement(<1..368)
                     /locus_tag="PLG98_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34082.1"
                     /translation="MKQTRVRPLFFKIFCALFAVTLFVAFSSSTGYAPTRMSDSELSE
                     IEGQAFFKIEHFPASSEFWDVVDPSGAPVGRKYNTGSQNVIRISMGIDVEAHAFIESQ
                     KMGYYNGGWDQDHTNFWVGDR"
     gene            complement(582..1448)
                     /locus_tag="PLG98_12250"
     CDS             complement(582..1448)
                     /locus_tag="PLG98_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34083.1"
                     /translation="MRTKLLFPLLVFVLLFCAMPVDAKTIVKIGRDVDISEDQQATGV
                     IAVGGQITVRGLVEKNVVAVGGSVVITGDAVVRGNVLCIGGVVVRGSGARIFGNVTEV
                     NSANFLAAVSSAFYEEEEEWSWLTDIIYFCFFTFLLMLALMLTFLFPRPLNAIIDSVA
                     AQKTKSFFWGILGALMIAPLFMLLALSFIGIPLIPLAFSLVLLAFIFGFLAMSALLGR
                     FVLTNFFRHHKPSLMKEILLGMILWWVIGWAPFYTGMMIKAAVITMGFGGVLVALIDR
                     GRNRQKTAHQAF"
     gene            complement(1461..>2063)
                     /locus_tag="PLG98_12255"
     CDS             complement(1461..>2063)
                     /locus_tag="PLG98_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOE34084.1"
                     /translation="LPLIIQSFGIWAYVIMFLVIFCETGLVVTPILPGDSLLFALGAF
                     AAQGALNIEILLISLCIAAVAGDTVNYSIGKFLGPKVFHYPDSRFFKREYLVRTHQFY
                     EKHGGKTIIIARFIPIIRTFAPFVAGIGTMKYLRFISYNVVGGISWICLFLLAGYYFG
                     GIPAVKRNFTLVIIAIIIISVMPGVIEYVRQKYFVSRAKN"
BASE COUNT          626 a          521 c          483 g          433 t
ORIGIN      
        1 cgatctccca cccagaaatt cgtgtgatcc tggtcccatc cgccgttata gtaacccatt
       61 ttctgagact cgataaacgc atgggcttcc acgtcaattc ccatggagat gcgaatgaca
      121 ttttggctgc cggtattata ttttctccca acgggagcac ccgacggatc gacgacatcc
      181 cagaattcac tggatgcggg aaaatgctct attttgaaaa atgcctggcc ttcaatttca
      241 gaaagctcgg agtcggacat ccgggtcggc gcataacctg tggatgaaga aaaggcgaca
      301 aacaacgtta ccgcaaacaa agcacaaaat attttgaaaa aaaggggcct cactcttgtc
      361 tgcttcaagt tcattttcat cccgaaacgg gctccttaag aaatcataat cagttttatg
      421 ttttaagggc taatattttt acgagtggtt taccatatac agaccaaaaa taaatgtcaa
      481 gcgcaaattt ttccggttta tttcataatc atcttattgc tttaacacct taaaaaaaca
      541 gagccgtctt tcagacaggc gcgaatgtct tttacgggtg actaaaacgc ctgatgagcc
      601 gttttttgcc ggttgcgtcc ccggtcgatg agggcaacca gcacgccgcc gaaacccatg
      661 gtgataacgg cagccttgat catcatcccc gtataaaaag gcgcccagcc gataacccac
      721 cataatatca tgcccagcag gatttctttc atcaatgatg gcttatgatg acgaaaaaaa
      781 tttgtcaaga caaaccggcc cagaagggcg ctcatcgcca ggaatccaaa aataaacgcc
      841 agaagcacaa gagaaaaagc gagcggaatc aaaggaatgc cgataaagga aagcgcaagc
      901 aacatgaaaa gaggagcaat catcagggcg cccagaattc cccagaagaa agattttgtt
      961 ttctgagcgg caacggagtc aataatcgca ttcaacggac ggggaaacaa aaacgtcagc
     1021 atcagcgcca gcatcaggag aaaagtgaaa aaacaaaaat aaatgatgtc cgtcagccag
     1081 gaccattcct cctcttcttc gtaaaaggcg gaagaaaccg cggccagaaa attcgccgag
     1141 ttcacttccg taacgtttcc gaaaatacgg gccccgctgc cccggacaac aacaccgccg
     1201 atacagagca cgtttccccg cacaacggcg tcaccggtga tcacaacgga gccgcccacg
     1261 gcgacgacat ttttttcaac cagcccccgg acggtaatct gaccgccgac ggcgatgacg
     1321 cccgtcgcct gctgatcttc ggaaatgtcg acgtccctgc cgattttgac aatggttttg
     1381 gcgtcaaccg gcattgcaca aaacaacaat acgaaaacga ggagggggaa aagcaacttt
     1441 gttctcatgt tgcaccgttc ttaatttttt gcacgcgata caaaatattt ctgccggaca
     1501 tattcaatga cacccggcat cacggaaatg atgataatcg cgatgatcac cagggtaaaa
     1561 ttccttttga cggcgggaat gccgccgaag taatatcccg ccagcagaaa aaggcaaatc
     1621 caggaaatgc ccccgacaac attatagctg atgaatcgca gatatttcat tgtgccgata
     1681 ccggccacga aaggggcgaa ggtgcggata atgggaatga accgggcgat gatgatggtt
     1741 ttgccgccgt gcttttcgta aaactgatgg gttcgcacaa ggtattcccg tttgaaaaag
     1801 cggctgtccg gataatgaaa caccttggga ccgagaaatt tcccgatgga atagttgacc
     1861 gtatcgcccg caacggcggc aatgcagagc gatatcagta aaatttcaat attcaacgcg
     1921 ccctgcgcgg caaaagcgcc cagggcgaac aaaagcgaat cgccgggcag aatgggtgtg
     1981 accaccaggc cggtctcgca aaaaataacg agaaacatga tgacgtaggc ccaaatgccg
     2041 aaactctgga taatcagcgg cag
//
LOCUS       DAOSME010000198         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_60690_length_2012_cov_17.955033, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000198 DAOSME010000000
VERSION     DAOSME010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60690_length_2012_cov_17.955033"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLG98_12260"
     CDS             complement(<1..383)
                     /locus_tag="PLG98_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE34085.1"
                     /translation="MKTTSKAKAANKPKRKSSAQKNPSEKTGKTQRKSSRKTDLSHLA
                     EVLIANSGNGIYIVQEGKFVFVSPLYQQLTGYSENELIGTDSLDNVHPDDRDRVRQEA
                     ISCLKAPHREPYEYRFITKQKEILWV"
     gene            complement(380..664)
                     /locus_tag="PLG98_12265"
     CDS             complement(380..664)
                     /locus_tag="PLG98_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34086.1"
                     /translation="MPSPAILTAAKESPLPGYFTVWSNVFERGGLKPGENFLVHGGAG
                     GIGYTAIQLAKAFGAKVFKEGVSSLHRAFFHVVISIHHRNTFSINLGEAT"
     gene            717..1040
                     /locus_tag="PLG98_12270"
     CDS             717..1040
                     /locus_tag="PLG98_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34087.1"
                     /translation="MAVVLKLSKVRQSARADDGGELMAEKAALLERIGNIAKITMNNP
                     ASLNSMIQALLDDTRAALRSDKLDLQAGIELETGIQLFCLGTEDHREGMDAFLAKRPP
                     VFKDR"
     gene            complement(1413..1667)
                     /locus_tag="PLG98_12275"
     CDS             complement(1413..1667)
                     /locus_tag="PLG98_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34088.1"
                     /translation="MKTKGIEELRPEYEFDYSKAVRGKYFKRLIEEGANVVVLEPEVA
                     KVFTSSAAVNEALKSLLDITKSTQRLTKHSTGRAKKLRAG"
BASE COUNT          498 a          480 c          502 g          532 t
ORIGIN      
        1 acccacagga tttccttttg cttggtgatg aaccggtatt cgtagggttc gcggtgcggc
       61 gcttttaaac aggagatagc ctcctgccgg accctgtccc ggtcgtcggg atgaacatta
      121 tccagggaat ccgtgccgat caattcgttt tcggagtatc cggtcagttg ctggtacagt
      181 ggactaacaa agacaaattt gccctcctgg acaatataga ttccgttgcc ggagttggca
      241 atcagaactt cggccagatg cgatagatcc gttttccggg aggatttccg ctgcgtcttt
      301 ccggttttct cggatggatt tttttgagcc gacgatttcc gtttaggttt gtttgcggcc
      361 tttgctttgg atgttgtttt catgttgctt cccccaagtt tatggaaaaa gtattccgat
      421 ggtgtatcga tatgactaca tgaaaaaacg ctctatgtaa agaagacaca ccttctttga
      481 agactttggc gccgaaggct ttggccagtt gaatggcggt atagccgatg cctcccgcgc
      541 cgccgtgaac cagaaaattt tcccctggct tgaggccccc gcgttcaaaa acattcgacc
      601 agacggtaaa atatcccggc aacggggatt ctttagcggc ggtgagaatc gccggtgatg
      661 gcaacttacc cctgccggcc gggcagggca aaacgtaacc gtcagacgga ctcatcttgg
      721 cggttgtttt aaaattgagt aaggtgcggc agtcggccag agccgacgac ggaggtgaac
      781 tgatggcaga gaaggcggca ttactggaaa gaatcggcaa cattgccaag attaccatga
      841 acaatcccgc ctcgcttaat tccatgatcc aggcgctcct cgacgatacc cgggcggcgc
      901 tgcggagcga caagctggat cttcaggccg gcattgaact ggagacaggg attcaactgt
      961 tctgcctcgg cacggaagac catcgggaag ggatggatgc cttcctggcc aaacggcccc
     1021 ccgtattcaa agaccggtag tgaaaatcat aaaagtcaca gcgaaacaag cttggttgcg
     1081 ggatggaaag acaggcttaa caaacgatat gactttgaaa aagtaccagc cggaaagggc
     1141 ggatttgctc atcaacacaa atagtatagt ggacatgcga tggcaaccca tagcgccgat
     1201 ggttcgccgg aatgtttttt gcgttaatct gcatacaaga gaatgttggc atccacgagg
     1261 atttcgcttt gtccagaaca tcgtcgtcta tggtaagtgt tgttctcata gtgtcatcat
     1321 cacatcatgt ggtgtgatgt caaggcatca tttcgatgtt ggagatgaca ttacctctca
     1381 cgcttcaact ccggatagaa cataacgaaa agctaaccgg cgcgcagctt tttcgcgcgt
     1441 ccagttgagt gttttgttag gcgctgtgtt gactttgtaa tatcgaggag cgatttcaat
     1501 gcctcattga ctgctgccga acttgtgaac actttggcta cttcgggttc gagcactacg
     1561 acattagcgc cttcctcgat cagcctttta aagtacttac cccggacagc ctttgaataa
     1621 tcgaactcat attcggggcg caattcctct attcctttag ttttcatgtc ttttcctctc
     1681 tgaaatggct gcaggtcgtg cactgataat tcgtatcgta tcccctcttt ccttatgcga
     1741 aacaacgagc aaatggcctt ggggtgaata accaacagta atgaacctat cctctgtaat
     1801 ggagtggtct tggtcatgaa acgttgccga taaaggatcg taaaaaatgg taacggcttc
     1861 gtcaaaagaa atggcgtgct ttctaatgtt tcccttttct ttttccctat ccccctcgaa
     1921 tttcacaatg taatccgata cgaatgactg accaaagata gatcgctttc aataatttct
     1981 ccaaaatgag atggccgacg aacaagcacc ag
//
LOCUS       DAOSME010000199         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_62328_length_1971_cov_14.138831, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000199 DAOSME010000000
VERSION     DAOSME010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62328_length_1971_cov_14.138831"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLG98_12280"
     CDS             complement(<1..853)
                     /locus_tag="PLG98_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOE34089.1"
                     /translation="MIRKTRIKDFTKPGGQVAELDAADWMKLPAPYERADKPLRKLTD
                     DQRKRMDATLDGVVAIGIAPPATPEEEKAFIDKFLAGVEKLFDAADNWTFLQPLFHTL
                     EYCVKCQICSDACPAYVASGRQDIYRPTMRPELLRRIISTYVKKEHPLMRKFKASDIE
                     LNGQALIRLAELAYRCTLCRRCAQVCLLGCDNALVSREIRKIFSQQMGIAPKEVHEKG
                     SAQQIKAGSSTGMTPPALADILEFTEEEIEEKTGKKIKIPVDKKGAEILLLHNAGEFL
                     AWPENLEA"
     gene            complement(850..>1971)
                     /locus_tag="PLG98_12285"
     CDS             complement(850..>1971)
                     /locus_tag="PLG98_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOE34090.1"
                     /translation="RSSMNFSRPARQKEPANWPGCPNRQVACNVASLSGFAGAGRRGS
                     AVNHEPEEKQTGKEDQSMGTFLIVLTYIAYVFIVAVYAIKVRKWIKLPTHIRWDLYPV
                     IHEEQYRYGGSYYEKPEWWMQPRPKNTGRSFWYALKDNFYMGEYFKRNPLYWLFLLPW
                     HLGFIFIILFHITCFFAAAAMVGGVTISATSSQPGGQIVYGLLMVFAVIAFIGGTFGC
                     IGMPVIRLVDRGLRSYAMPMNYFNYVFFLIVYASGLYSWLALDPTLAEYRMYWVGLIT
                     LTPPELNPGTIFHILLFDIFLIYLPFTRSTHYITRFLAYFKIRWDDEPNFRGSELEKK
                     LNMLLEQKLSWDAPHMSKGKNWGEAAVSSGLPEPKKDNV"
BASE COUNT          441 a          542 c          529 g          459 t
ORIGIN      
        1 aggcttccag attctccggc caggccagaa actcgccggc attgtgcaaa agcaatattt
       61 cagcgccttt tttatccacg ggaattttga ttttttttcc tgtcttttct tcaatctctt
      121 cttcggtgaa ttcgagaatg tccgccagcg ctggcggcgt cattcccgtg ctggaacccg
      181 ccttgatctg ctgggcggag cctttttcat ggacttcttt cggggcaatc cccatctgct
      241 ggctgaaaat tttacggatt tcccggctga ccagggcgtt gtcgcagccc agcagacaaa
      301 cctgcgcgca gcgccggcac agcgtgcagc gataagccag ttcggctaag cgaatcaggg
      361 cctggccgtt gagttcgatg tcgctggctt tgaatttgcg catcagcgga tgttcctttt
      421 ttacatacgt actgataatt cggcgcagga gttccggcct catcgtcgga cggtagatgt
      481 cctggcgccc gctggccaca taagccggac aggcatcgga acagatctgg catttcacgc
      541 agtattctag cgtgtgaaaa agtggctgga gaaaggtcca gttgtctgcc gcatcaaaaa
      601 gcttttcgac acctgccagg aatttatcga taaacgcctt ttcctcctcc ggcgtggctg
      661 gcggcgcaat gccgatcgcc acgacgccgt cgagcgttgc gtccatccgc ttgcgctggt
      721 catccgtcag tttccgcaac ggtttgtccg cacgctcgta cggagcggga agcttcatcc
      781 aatcggccgc atccagctcg gcaacctgtc cgccgggttt tgtaaagtct ttgatacgcg
      841 ttttcctgat catacattat ccttttttgg ttccggcaga ccagacgaaa cggcggcttc
      901 accccagttc ttgcctttcg acatgtgagg agcgtcccag gaaagctttt gctccagaag
      961 catgttcaat tttttttcca gttcactgcc gcgaaaattc ggctcgtcat cccagcggat
     1021 tttaaaataa gcgagaaacc gcgtgatgta atgcgtagag cgggtaaaag gcagatagat
     1081 caggaaaatg tcgaacagaa gaatgtggaa aatcgtgccc ggattcagtt cgggcggcgt
     1141 cagtgtgatc agccccaccc agtacatccg gtattcggcg agcgtcgggt ccaaggcgag
     1201 ccaggagtaa agaccgctcg catagacgat caggaagaaa acatagttga agtaattcat
     1261 cggcatggcg taggaccgca ggccgcggtc caccagccgg atgaccggca tgccgatgca
     1321 gccgaaggtg ccgccgataa aggcaatgac ggcaaagacc atcagcaggc cgtacactat
     1381 ctgccctccc ggttgcgaag acgttgcgga aatcgtcacg ccgccgacca tcgcggccgc
     1441 ggcgaaaaag cacgtgatgt gaaaaagaat gatgaagata aaccccaggt gccacggcaa
     1501 aaggaaaagc caatacagcg ggttacgctt gaagtattcc cccatgtaaa aattatcctt
     1561 gagggcatac cagaagctgc gtcccgtgtt tttcggtcgc ggctgcatcc accactccgg
     1621 cttttcatag tacgagccgc cgtagcggta ctgttcttca tggatgacgg gataaagatc
     1681 ccagcggatg tgcgttggca gttttatcca cttacggacc ttgatcgcgt acaccgccac
     1741 gataaacaca taggcgatgt atgtcagaac tatcagaaac gttcccatgg attgatcttc
     1801 ctttccagtt tgcttctctt ccggttcatg attaacggca ctgccgcgcc ggccggcgcc
     1861 ggcaaaaccg gataacgacg cgacgttaca cgcaacctgt cggtttggac agcccggcca
     1921 gtttgcaggc tccttttgcc gggccggtcg ggaaaagttc atagagcttc t
//
LOCUS       DAOSME010000200         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_63328_length_1947_cov_17.124736, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000200 DAOSME010000000
VERSION     DAOSME010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63328_length_1947_cov_17.124736"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..696)
                     /locus_tag="PLG98_12290"
     CDS             complement(58..696)
                     /locus_tag="PLG98_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOE34091.1"
                     /translation="MENKNCKNKRVHVVLFDFGGVLSEEGWKKGLRIIAKANGVHPDS
                     FLQTAADTIYETGYILGKGSQSDFWKSLKTKTGIRGDVASLSGELISRFALNDAMMET
                     VRNLKSNNFIVGILSDQTDWLDGLNARFDFFRHFDYVFNSYHLGKGKRDPSLFDDIAG
                     LLRETPENILFIDDDPGNIQRARQKGWKAILYTDFKSFREEINEIIQERTLV"
     gene            complement(1242..1850)
                     /locus_tag="PLG98_12295"
     CDS             complement(1242..1850)
                     /locus_tag="PLG98_12295"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HOE34092.1"
                     /translation="MRSNRLPNHNTHCSRCYQRREICLCPIIPAVETSVEFLILRHIY
                     EAEKPSNTGRLAALALTNSKLISCGGGQRIGLACVDDDLLNAPGTWLLWPDGAGTLPR
                     TSDRQSPARVVVIDATWQQARRLYSSLPAFWRMPRLALEAPVAQRKRLRDQHCPNYMS
                     TLEAVAAAVAMLEGGGKNVPLDNLYDELVRRRRSLRWGSKES"
BASE COUNT          482 a          524 c          415 g          526 t
ORIGIN      
        1 cgcagacaat cctccacctg acctgttatg ttgataaact cgcgtctgtg cgcggtatta
       61 aaccagagtt ctttcctgta tgatttcatt aatctcctcc cggaatgatt taaaatccgt
      121 gtaaagaatc gccttccatc ctttttgtcg ggcacgttga atattgcccg gatcgtcatc
      181 aataaataaa atgttctccg gtgtttctct cagtaagccg gcaatatcat caaaaagcga
      241 cggatctctt ttccctttgc cgagatgata actgttaaag acataatcga aatgcctgaa
      301 gaaatcaaaa cgtgcattca gcccgtccag ccagtccgtc tgatcgctca gaatgccgac
      361 aataaaattg tttgacttca gattccggac agtctccatc atggcatcat tgagcgcaaa
      421 cctcgaaatc agttcacccg ataacgaagc gacatccccc cggatgccgg ttttcgtttt
      481 gagagatttc cagaaatcgc tttgcgagcc ttttcccaga atataaccgg tttcataaat
      541 cgtatcggcg gctgtttgaa ggaaactgtc ggggtgaact ccgttcgctt ttgcgatgat
      601 tctaagccct tttttccaac cctcttccga gagcacgccg ccaaaatcaa acaaaacgac
      661 atgaacacgt ttatttttac aatttttatt ttccaacaat ttataactcc ctgaactcgg
      721 ccatccttct tatcattaca tccattttga ccgttttcca tttttcattt caaacatcct
      781 ttgatcaccg aaatgacaca aagaaccatc aaccattaac tatcaactat caactaaata
      841 attttgtcgg acatccctcc gtttgtcatg cccgagtggg cttgtcgggc atccagtctt
      901 cttttttcct gtataccggc caggcccgcg ccggtatgac tcggaggtgt tgtgttactt
      961 cgaacgaatt taagaagtct taaagatttc tcgccactgc gtttctcgaa attacacgga
     1021 ggggtgcttt gcacccgacg tgacgctccg atttaacgtg aaaaaaaata tccaataacg
     1081 caaagcatga taaacacggc gatggctgaa taaagtatga tgcgaagttt tgttatgtct
     1141 ttcatgattc attttccttg acggcttgta ttctattgtg aatttcgggc aggaagaccg
     1201 gaacctttgc tgcctgatcc ggctatatcg gcaaaaaaac gtcaggattc tttgctcccc
     1261 catcgcaagg aacgtcttcg gcgaaccaat tcatcgtata aattatccag cggcacattt
     1321 ttcccgcctc cctcaagcat cgctaccgca gccgccaccg cctccagggt ggacatataa
     1381 ttcggacagt gctgatcgcg caatctcttt ctttgggcga caggcgcctc cagggccagg
     1441 cgaggcatcc gccaaaaagc gggcagactg ctgtacaacc ggcgggcctg ttgccatgtt
     1501 gcatcaatca ccaccacccg agccggggat tgcctgtcgg atgtccgcgg aagcgtgccg
     1561 gctccgtccg gccacaacag ccaggttccg ggagcattca gcagatcgtc atccacgcac
     1621 gccagaccga tgcgctgacc tcctccgcag gaaatgagtt ttgaattcgt aagcgccagc
     1681 gcagccagac ggcctgtgtt gctgggcttt tccgcttcgt aaatatgccg gaggatcaga
     1741 aattccacgc tggtttccac cgcggggatg atgggacaca ggcagatttc ccgacgctga
     1801 taacagcgtg agcagtgtgt gttatgattc ggcaacctgt tcgaacgcat tcaaaatact
     1861 ttcacattat tttgttgccc agtatacgta aagccttttt atctgtcaac ggccccgccg
     1921 tctaaaattc tgagctgcca tccctgc
//
LOCUS       DAOSME010000201         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_66178_length_1885_cov_25.271585, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000201 DAOSME010000000
VERSION     DAOSME010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66178_length_1885_cov_25.271585"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..>1885)
                     /locus_tag="PLG98_12300"
     CDS             complement(329..>1885)
                     /locus_tag="PLG98_12300"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOE34093.1"
                     /translation="RSGYDTVIRNGTVYDGTLANPFIADIGIRGDRIAAIGSNLGEGA
                     QSIDARGLIVTPGFIDVHNHGDLTFQRLGKNVVTTASGIPSLAENRSYAYQGVTTIVT
                     GNCGFGYTNVDGFYDILDKVHFGSNVYHLAPHGAIREDLFGPNQPKKLSPQQLEAMKR
                     RVAEEMEKGAVGLSTGLEYAPGALTETEELVELAKIVKRYNGLYVSHIRDLTGAIYAD
                     GRIGVIEALKEAIAIGRKAEVSVHLSHIQINEPINAMTAKPMIELLTKARAEGLDITA
                     DAYPYDAGVTWITMLTPAKYKTDKGIKEEYKDLKGRAELKKAVEKTFAYLGPERTTII
                     IYPERPHYEGKTIEEIADMEKKAPSDLYVDIVCSNDAPQAIMNDQDIRNVREFLMCTD
                     FVFVSSDGWTTIKDVGKPHPRCYGAYPRMIKKFVMEEKILDLSAVIRAMTSLPAGKFR
                     MKDRGKLAVGYIADIAVIDPQKLADHATYNNPHQYSTGVEHLFVNGTPSISQGKNTNQ
                     RGGRASKRQM"
BASE COUNT          401 a          507 c          441 g          536 t
ORIGIN      
        1 aacctgcggt cattgaagcc gacgcggaag attccggcga tgactgggaa aaacgtgttc
       61 tatgttcgga cgaggcctgc atcggtgtca ttggcccgga tggccgttgc aaggagtgcg
      121 gaaaatccct gaaacaaact catcttgtta gccaatgatg tatgaaagcg gcaatcagca
      181 aaactattcc aattttcttc gcgttataac gccatgatga gtcgcgacgg taaggagtcg
      241 gttccatcaa gtggttgggc gatgctctgt gctgtaaatc tcacatttca atatttcaag
      301 agcaccgtct tacagtcgcc gccgcgcttt acatttgcct tttcgaagcc ctgcctcccc
      361 gttgatttgt gtttttgccc tggctaatgg aaggtgtccc gttgacaaaa agatgctcca
      421 ctcctgttga atactgatgg ggattattat acgttgcatg atctgcgagc ttttgcgggt
      481 ctataactgc aatgtccgcg atatacccca cggccagttt ccctctgtct ttcatcctga
      541 actttcctgc aggcagcgag gtcatggctc tgataacggc agacaagtcc agaatctttt
      601 cttccatgac aaacttcttg atcattcttg ggtatgcgcc atagcatcgc ggatgcggtt
      661 tccccacatc cttgatggtt gtccatccgt ccgagcttac aaatacaaaa tcagtgcaca
      721 tcaggaattc ccttacgttc cgaatgtctt gatcgttcat tatagcctgc ggcgcatcat
      781 tggaacagac tatatccacg tacaggtcag aaggtgcttt tttctccatg tcggcaattt
      841 cttctatggt tttcccttca tagtgcggtc tttcaggata tatgatgatc gtcgtcctct
      901 ccggtccaag gtatgcaaat gtcttttcaa ccgccttctt tagctcggcc cggcctttta
      961 aatctttata ttcctccttg atccctttgt cggttttgta cttcgcggga gtcagcatgg
     1021 ttatccaagt tacaccggca tcatacgggt aagcatcagc ggtaatgtcc agcccttccg
     1081 cccgtgcctt ggtgagcaat tcgatcatcg gctttgccgt catggcattg atcggttcat
     1141 tgatctggat gtgggaaaga tgaactgaga cctccgcctt tctgccaata gcgatcgctt
     1201 ctttcagcgc ttcgatcacc ccaatccgcc cgtccgcgta aattgccccc gttaaatccc
     1261 gaatatgaga tacatacaac ccgttatatc tcttaactat ttttgccagt tcaacgagtt
     1321 cttcagtttc cgtgagtgca ccgggagcat attccaggcc tgttgacaga cccacggcac
     1381 ccttttccat ttcttcggca acccttctct tcattgcctc aagctgctgt ggggacagct
     1441 ttttcggttg atttgggccg aagaggtctt ccctgatcgc cccatgggga gccagatggt
     1501 acacgttgct cccgaagtgc accttgtcga ggatgtcgta aaacccatcg acatttgtat
     1561 agccaaaccc gcagttcccc gtgactatgg tggttacacc ctggtaggca tagctgcgat
     1621 tttccgcaag gctgggtatt ccggatgcgg tggtcactac gtttttcccg agacgctgaa
     1681 aggtgaggtc gccgtgatta tgaacatcga taaagccggg cgtcacgata agccctctgg
     1741 catcgatact ttgtgcacct tcgcccaggt tgctgccgat ggcagctatc ctgtcacccc
     1801 gaatcccgat gtcggcaatg aaaggatttg ccagagtacc gtcatagacc gtcccgttcc
     1861 gaataacggt atcgtacccg ctcct
//
LOCUS       DAOSME010000202         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_67199_length_1865_cov_23.535359, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000202 DAOSME010000000
VERSION     DAOSME010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67199_length_1865_cov_23.535359"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLG98_12305"
     CDS             <1..312
                     /locus_tag="PLG98_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34094.1"
                     /translation="KEDIMPFEMMKKVMLTGIGLALKTRSEMETVAKDIIKKSKMSEA
                     EGRKFVADLTKKYEQSRRDMEKTIQKGIADYMASADIASRKELNALKKEIGNLKKARK
                     K"
     gene            380..>1865
                     /locus_tag="PLG98_12310"
     CDS             380..>1865
                     /locus_tag="PLG98_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOE34095.1"
                     /translation="MLSIKKIGVLGRTYRNFSRYRQILSILFRYGFDDLVERLKIDQY
                     IEIGLQMISRHQRENVERLSRPERVRLLFEELGPTFIKFAQILSTRPDLVPADVIQEF
                     EKLQDNVPPFPFSEAKAIIESELGGDLDNFFLSFEEKPLASASIAQVHKAVLNDGEVV
                     AVKIQRPGLKKIIEVDLEIMLHLASLMEKNIEEISFQKPTRVVEEFARTLERELDFTT
                     EASSMERVAGQFLSDRTIYIPKVFSELSGRRVLTMEYVDGIKVSDMEKLEKAGMDLKK
                     ITSRGAHFVIKQVFEFGFFHADPHPGNVFVLPNNVICVLDFGMTGSVDKRQRALFVDI
                     METLGRKDAEQCARLMLELGEYDDEPDIRLLEREIDDFMGRHLFKSLKDIDLARLIQD
                     LLDIATSNKIRIPPVIFLMMKAFAAMEGIARLLDPEFDMIAYAEPFIKRAKLARYAPE
                     KIAADLLTVVSESMHMIQALPREVLQVTRLIRQNKMTINMEMRWI"
BASE COUNT          524 a          432 c          466 g          443 t
ORIGIN      
        1 aaggaggaca ttatgccgtt tgaaatgatg aaaaaagtta tgttgacagg aatcggcctt
       61 gctctgaaaa cccgctcgga aatggaaacc gtggccaaag acatcatcaa aaaaagtaag
      121 atgagcgaag cagaaggcag aaaatttgta gccgatttga caaagaaata cgagcagtcc
      181 agacgtgaca tggaaaaaac aattcaaaag ggcatcgccg actacatggc cagcgcggat
      241 attgccagca ggaaagaact caatgccctt aagaaagaga tcggcaattt gaaaaaagcc
      301 cgcaagaaat aagtccgggt atcctggatc tgttgcccgg cgcaaacggc ttcagaacgt
      361 gcaagaggat ttcagcggaa tgttaagcat caaaaaaata ggtgtgctgg gtcggacgta
      421 ccgcaatttc agccgctatc gacaaatcct ctccattctt ttcagatatg gatttgatga
      481 tctggtggaa agactgaaaa tcgatcagta tatcgagatc ggtcttcaga tgatttcccg
      541 tcatcagcgc gaaaatgttg aaagattaag ccgtccggaa cgggtgcggc tgctttttga
      601 agaactcggt ccgacattca tcaaattcgc ccagattctt tccacgcgtc cggaccttgt
      661 tcctgcggat gttatccagg aattcgaaaa actccaggat aacgtgccgc ctttcccctt
      721 ctccgaggcg aaagcaatta tcgaatccga gctcggcggt gatctggata attttttctt
      781 gtcgtttgag gagaagcctt tggcttccgc ctccatcgcg caggtgcata aggccgtgct
      841 caatgacggc gaggttgttg ccgttaaaat ccagcggccg ggactcaaaa aaatcatcga
      901 agtcgatcta gagatcatgc tccatctggc gtcgctgatg gagaaaaaca tcgaagaaat
      961 atcatttcag aagcccaccc gtgtcgtcga agaatttgcc cgaacattgg aaagagagct
     1021 ggattttacc actgaagctt ccagcatgga acgtgttgcc ggacagttcc tcagcgacag
     1081 aaccatctac attcccaaag tcttttcaga actgtccggc aggcgggttc tcaccatgga
     1141 atatgtggac ggcatcaagg tgtcggatat ggaaaaactt gaaaaggccg gcatggatct
     1201 taaaaaaatc acctcccgcg gcgctcattt cgttataaaa caggtttttg aattcgggtt
     1261 tttccatgcc gatccgcatc cgggtaacgt ctttgtcctg ccgaacaacg tcatttgtgt
     1321 tctcgatttc ggcatgaccg gttccgtgga caaacggcaa cgcgcactct tcgtggatat
     1381 tatggaaacg ctcggcagaa aagatgcgga acaatgtgcg cggctgatgc tggagcttgg
     1441 agaatacgat gatgaaccgg atatccgcct gctggaaaga gagattgatg attttatggg
     1501 caggcatctt ttcaaatcgc tcaaagacat tgatctggcc cgtttgatcc aggatttgct
     1561 cgatattgcc accagcaata aaatcagaat ccccccggtt atatttttaa tgatgaaagc
     1621 cttcgccgcg atggagggca tcgcccgcct gctcgatccg gaattcgata tgattgccta
     1681 cgccgagcct tttattaaac gggcgaaact ggcccgatat gcgccggaaa aaatcgccgc
     1741 ggatttattg accgttgtgt ccgaatccat gcatatgatt caggcccttc cccgggaagt
     1801 gttgcaggta accagactga ttcgtcagaa caaaatgacc atcaatatgg aaatgcgatg
     1861 gatcg
//
LOCUS       DAOSME010000203         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_67365_length_1862_cov_4.065855, whole genome shotgun sequence.
ACCESSION   DAOSME010000203 DAOSME010000000
VERSION     DAOSME010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67365_length_1862_cov_4.065855"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLG98_12315"
     CDS             <1..468
                     /locus_tag="PLG98_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34096.1"
                     /translation="MNNPLSPDAFKINDQISIEAEFNLISKSYGTGRGDVFTSPVNVL
                     PWDASKKEKIPSVITADDYFQKNMKLGATPVTIVGAVSMKRVDANEAWIILGAAGGKI
                     HGELHKSKIDSEVKQQYESLQVGDRVAFKGSFNMEMGNTAYFTIEQIILNPPY"
     gene            476..979
                     /locus_tag="PLG98_12320"
     CDS             476..979
                     /locus_tag="PLG98_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34097.1"
                     /translation="MQPSIECKPYQRRWRDWAVDRNLEDLWLERLNVLETLDLVSICE
                     GHLDARPNSVKRRPCIILRPKKDYLMPLTVGWYDLKAALAAEIKRIWPDAETIVEFEI
                     QHRLVQNGDDPEDIEDIIIRITSKRKRDAASLPEWAALWFQHSLIRIGIFDGFLKTLI
                     IVNPEQG"
     gene            987..1520
                     /locus_tag="PLG98_12325"
     CDS             987..1520
                     /locus_tag="PLG98_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34098.1"
                     /translation="MKNSPTKHFYQQSLFLRWASRNRRLFSHQPYLLRRKKGSFFIGF
                     RGVSKHITCCFTKVGAIMICVDYRNTNFDIIREFDLFEERTREGQWLCSMCRDHPNPD
                     KTEPLIEYEERKKLWIEHSFAPLAAWTRKSFTKDAMLCLYRSGGTTWAAIEKKPHLTK
                     TEQSPYLFKKFPVLTVR"
BASE COUNT          540 a          427 c          469 g          426 t
ORIGIN      
        1 atgaataacc cgctcagtcc cgatgccttc aaaatcaacg atcaaatttc aatagaagcg
       61 gaatttaacc tgatcagcaa atcatacggc accggcaggg gcgatgtctt taccagtccg
      121 gtaaatgtcc ttccctggga tgcctcgaag aaagagaaaa taccttcggt cattacagcg
      181 gacgattatt ttcagaaaaa catgaagttg ggcgccacgc cggttacgat tgtcggcgct
      241 gtgtcgatga aaagagtgga tgcaaacgaa gcctggatca tactgggagc agcgggagga
      301 aagattcacg gcgaactgca taaatcaaaa atcgacagcg aggttaagca gcagtacgaa
      361 agccttcagg tgggcgaccg cgtggctttc aagggcagct tcaacatgga gatgggcaat
      421 accgcgtatt ttaccatcga gcaaatcatc ctgaatccgc cttactgaaa tgaccatgca
      481 gccgtcaata gaatgcaaac cctatcaaag acgctggcgg gattgggccg ttgaccggaa
      541 tctggaagac ctctggctgg aacgtctgaa cgtactggaa acgctcgatc ttgtcagcat
      601 ctgcgagggg catcttgacg cccggccaaa ctccgtcaag agaagaccct gcatcatcct
      661 cagaccgaaa aaggattacc tgatgccgct taccgtcggc tggtatgatc tgaaagcagc
      721 gctggccgct gagattaaga gaatctggcc ggatgctgaa acgattgttg aatttgagat
      781 ccagcaccgg cttgtccaaa atggcgatga tccggaagat attgaagaca tcattatcag
      841 gattacatca aaacggaaaa gagatgcggc gagcttgccg gaatgggccg ccttgtggtt
      901 ccagcattcg ttaatcagga ttggaatctt cgacgggttt ttgaaaacgc tgatcattgt
      961 caatccagaa caaggataaa aaaaagatga aaaacagtcc aacaaagcat ttctaccagc
     1021 agtcactgtt tttgcgttgg gcttcgcgca accggcggtt gttttcccat cagccctact
     1081 tgctacggcg gaagaagggc tcctttttca tcggctttcg tggtgtcagc aagcatatta
     1141 cctgttgttt taccaaggtc ggtgccatca tgatctgcgt tgactaccgg aatactaatt
     1201 ttgacatcat aagggaattt gacctttttg aagaacgtac cagggaaggc caatggctgt
     1261 gcagcatgtg ccgcgaccac cccaatccgg acaaaaccga acccttgatc gaatatgaag
     1321 agcgcaagaa attgtggatc gaacattcct ttgcgccact ggcagcctgg acgcggaaat
     1381 ccttcacaaa agatgcaatg ctttgcctgt atcgctccgg tggcacgacc tgggccgcaa
     1441 ttgaaaagaa accacatctg acgaaaaccg agcaaagccc ttacttattc aagaagttcc
     1501 ccgttttgac ggtgaggtaa ggaaatcgga aataatcttg atggtcacat caagcgagag
     1561 cacgggtatc gaggtgggaa ttctatgttg cggaatctat tcttttattg tattaaataa
     1621 cttaaggacc atctggttac aatcgcagga taaaagagca gtgcccacgc acgaaatgca
     1681 ggacttgaat catgagtgaa aagacaatcc aatactggat gttgataaaa cacaggggct
     1741 gctccaatgg ctaaaagctc ggattttgta gaaaaagaat tgaaagactt cgttgccctg
     1801 atcaggaaag catacgacat ttacgctgtt gtcctctacg gatcttatgc ggaaggccgg
     1861 gc
//
LOCUS       DAOSME010000204         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_67858_length_1851_cov_19.425947, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000204 DAOSME010000000
VERSION     DAOSME010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67858_length_1851_cov_19.425947"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1851
                     /locus_tag="PLG98_12330"
     CDS             <1..>1851
                     /locus_tag="PLG98_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE34099.1"
                     /translation="IYETSVTLIKNAQGKAIGFRGVSRDITQRKNMEEALRQSEERYR
                     SIIENMTDGYFEVDLYGNFTYLNDAQCQNLGYTREELIGMSYKKYADEKISKELAHLF
                     YKIYKTEIPVKSYDLSFIRKDGTRAYNEISVTVLKNAKGEIIGFRGIARDITERKQME
                     ESLRQSEERYRTVIEQIEDGYFETDLRGNFTFVNEAESRNLGYTGEELIGMNRHQYTD
                     EKNVKALFRLFNNVYKTGIPVKTHEVELIKKDGAIAYNEISVNLIRNKNGEAIGFRGI
                     SRDITERKRQQEKIHYLATHDSLTGLPNRMMLNQLLSHAINAAKRHNRKLAVFFIDLD
                     RFKIINDMLGHEAGDRLLQEIALRFRQALRSMDIVARMGGDEFVVLIEEIENCEQIST
                     VAHKLMAEAAKPFSLMSEEFRVTASIGISIYPKDGETEQSLLKNADIAMYFAKEEGKN
                     NFQFYSKDIKSHSIEKLSIETQLRFALERNELFLMYQAKVDFKTALITGVEALLRWNN
                     PTLGAITPLQFIPVAEETGLIVPIGRWVLNTACGQNIAWQKEGLPPVCTSVNLSLRQL
                     LDEHLLYDIENALKNSGLSPSLLELEITESMIMHNPGRMISVLGKIKELG"
BASE COUNT          589 a          418 c          405 g          439 t
ORIGIN      
        1 taatttacga aacatcggtt actttgataa aaaatgctca aggcaaagct attggatttc
       61 gcggcgtttc ccgcgacatc acccagcgta aaaacatgga agaagctctt cgccagtccg
      121 aagaaagata tcgcagtatt attgaaaaca tgacggacgg ttattttgaa gtggatcttt
      181 acggaaattt tacatatctt aatgacgcgc aatgccagaa tctcggatat acccgggaag
      241 aattaatcgg gatgagctac aaaaaatatg cggacgaaaa aatcagcaaa gagctggctc
      301 acctctttta taaaatttat aaaacggaaa taccggtaaa atcctatgat ctttctttca
      361 tcagaaaaga cggaaccagg gcatacaacg aaatttccgt aacagtgctc aaaaacgcaa
      421 agggagaaat catcggcttc cgcgggatag cacgcgatat caccgaacgc aaacaaatgg
      481 aagaatctct tcgccagtcc gaagagagat accgcactgt tattgaacag attgaggacg
      541 gctatttcga aaccgacctg cgcggaaatt ttacttttgt caacgaagcg gaaagccgca
      601 atctcggcta caccggtgaa gaactcatag gcatgaaccg gcatcaatac actgatgaaa
      661 aaaacgtaaa agccttattc cgtcttttca ataacgttta taaaaccggg atcccggtca
      721 aaacacacga agttgaactt ataaaaaaag acggcgcaat cgcttataac gaaatttcgg
      781 taaatctgat aagaaacaaa aatggagaag caataggttt tcgcggcatt tcccgggaca
      841 ttaccgaacg taaacggcaa caagaaaaaa ttcattatct ggccacccac gattctctta
      901 ccggtcttcc caaccgtatg atgctcaacc agcttttaag ccatgccatc aacgcggcaa
      961 aaaggcataa tcggaaactg gccgtttttt tcattgactt ggatcgtttt aaaattatca
     1021 acgacatgct gggacatgaa gcgggagacc ggctcctcca ggaaatcgcc ctgcgtttcc
     1081 gacaagcttt gcgttccatg gatattgtcg ctcgtatggg aggggacgag tttgtcgtcc
     1141 ttattgaaga aatagagaac tgtgagcaaa tatccaccgt tgcccataaa cttatggctg
     1201 aagcggcaaa acctttttcg ctcatgtccg aggaattccg cgttacagcc agcataggca
     1261 tcagcatcta cccaaaagat ggagaaacag aacaaagttt gttgaaaaac gccgatattg
     1321 cgatgtactt tgccaaggaa gaaggcaaga acaattttca gttctattcc aaagatatca
     1381 aatcacattc tatcgaaaag ctatcaattg agacacagct tcgcttcgcc ctggaacgaa
     1441 acgagctctt tttaatgtat caggccaagg tggactttaa aaccgcctta ataaccggtg
     1501 tggaagccct tttgcgctgg aacaacccga cattgggagc gattacaccg cttcagttca
     1561 ttcccgtggc tgaagaaaca ggtctaatcg tgcccatcgg cagatgggtt ttaaacacgg
     1621 cctgcgggca aaacattgca tggcagaaag aaggtttgcc gccggtttgc acgtctgtaa
     1681 acctgtcgct gcgccagctg ctggacgaac atcttcttta tgatattgaa aatgcgctga
     1741 aaaattcagg tctgtctccc agccttttag agttggaaat caccgaaagc atgataatgc
     1801 acaatcccgg ccgcatgatt tccgttctgg gcaaaataaa agaactgggc g
//
LOCUS       DAOSME010000205         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_68015_length_1848_cov_24.602900, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000205 DAOSME010000000
VERSION     DAOSME010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68015_length_1848_cov_24.602900"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLG98_12335"
     CDS             complement(<1..240)
                     /locus_tag="PLG98_12335"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOE34100.1"
                     /translation="MKKTKQTTAAERQYLIEKEKFVPVSQYFGEDTFNRKAMRERLPK
                     EAFKKLMDAIEARKTIDAKTADVVAEAMKKWALEKG"
     gene            806..1639
                     /locus_tag="PLG98_12340"
     CDS             806..1639
                     /locus_tag="PLG98_12340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE34101.1"
                     /translation="MMSGFSNKLAFMTGGSSGIGLATANLLAAQGCNLVLFARGQKML
                     DEACKTVKTKMKSSSQKVNAVSMDVADNDDVQQKIKLAVEKFGVPDILINSAGVGSGD
                     YFENISYEQFDRLLKINVYGTRNTVSAILPFMKQKGGGHIVNIASVAGLIGMYGYSLY
                     CTSKYAIVGFSECLRSELKRFNINVTLICPPEVKTPFIEEEAKTLPPEARIVKNLAGL
                     LTPEQAAKAIVRGIQGRKFLFVPGLMARSLLFWHRISNGWLTRTPSDLIIKLAARRSR
                     Q"
BASE COUNT          459 a          472 c          419 g          498 t
ORIGIN      
        1 ccctttttcc agagcccatt tcttcatggc ctcagccacg acatcggctg tctttgcgtc
       61 gatggttttg cgcgcttcga tcgcgtccat cagcttcttg aaagcctctt tgggaagtct
      121 ttccctcatg gcctttcggt taaaggtgtc ctccccgaaa tactgcgata cgggaacaaa
      181 cttttccttt tcgatcagat attgtctttc tgccgcggtg gtttgtttcg tttttttcat
      241 aatcgtatca gcccctctgt ttaaaatagt gtattccggt catttgtttt cctcgatctt
      301 ccggacaccg gtcgtgatct gccggcgtgc aaatccctgc ccgtacaact atctccgttt
      361 agcttatatc catgcacgtt tcgccgggaa acattacccc cttttcaacg gacgccggac
      421 tcaccggttg tgatgtttcc tatactccag aaaagacgtt tttttcaagc ctttatccgg
      481 aatatctttg acctgcggac atttcgcaaa atccgcactc atcgcacgca attgtactac
      541 agaaagctgc gaatcgaccg aaaaatgccc tgagagaaac tccgaccgga aaagaatgcc
      601 ccagcctgat caatccaact tgttaaaatt cttctgcaga aaaatgaatt ttaggatata
      661 aagaaagtcc aatcgcacaa aaaccgccgc catgaagatg ctttctttcg cagaatcgtg
      721 tgcagcgatt gaaacaatcc gggcgcgcaa gaaccattcc ggatcaggct tctttcagat
      781 cagattatga catgtcggga ggacaatgat gagcggcttt tccaataaac tcgcctttat
      841 gaccggaggg tccagcggca tcggtctggc aacggccaat ttactcgcgg cgcagggatg
      901 caaccttgtt ttatttgccc gagggcagaa gatgctggat gaagcatgca agacggtcaa
      961 aacaaaaatg aaaagctctt ctcagaaggt caacgccgtc tccatggacg tcgcggacaa
     1021 tgatgatgtt cagcagaaaa tcaaactggc cgtcgaaaaa ttcggcgttc ccgatatttt
     1081 aatcaacagc gccggtgtcg gttccgggga ttattttgaa aatatttcct acgaacaatt
     1141 cgacaggtta ctcaaaatca atgtgtacgg cacccggaat accgtaagcg ccattctgcc
     1201 tttcatgaaa caaaagggcg ggggtcacat cgttaatatt gcatccgttg ccggattgat
     1261 cggcatgtac ggttattctc tgtactgtac aagtaaatat gccatcgtgg gattttcgga
     1321 atgcctgcgc agcgaactca agcggtttaa tattaacgtg acgctcattt gtccgccgga
     1381 agttaagact ccttttatcg aagaagaagc aaagacactc ccgcccgagg cgcggatcgt
     1441 aaagaatctt gccggtctgc tgacaccgga acaggcggca aaggcaattg tccgcggcat
     1501 ccaaggaagg aagtttctct tcgtacccgg cttgatggcc aggtctcttc tgttctggca
     1561 ccgcatcagc aacggttggc ttacgcgaac accttctgat ttgattatca aactcgccgc
     1621 cagacgatca cgccaataaa gatatctgca aaaatattct gtctgatgcg atgttttttg
     1681 acgtggagtt tctttctatc gctctttatt tttctttttt ttcttcttga ccggttgaac
     1741 gatgccggaa ggcagcggca gagaggtcat cagtcgatac gcaatagcca ctcccagcac
     1801 aaacaggatc atgcttccat attgtccggg cgtgagcgcc ccataccg
//
LOCUS       DAOSME010000206         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_70912_length_1795_cov_19.037356, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000206 DAOSME010000000
VERSION     DAOSME010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70912_length_1795_cov_19.037356"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLG98_12345"
     CDS             complement(<1..619)
                     /locus_tag="PLG98_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE34102.1"
                     /translation="MRKMMWLKAIVLAVLLMLPLALTAPVQAARSIDPVVSTEWLQKN
                     LNNANLVVVDVRKLEEYKAGHIPGAVNIVYGTWAVKKGDLLNELPAIDDLADAMGSAG
                     IDQNSLVVIVGKTDKVPDQFDMTRVAWTLKYLGVPNVAILNGGQNHWVKENKPVSQDM
                     ARPKEKSFTPSVNKNLFVDKASLAGKIGQAVILDNRAPAFFEGKEK"
     gene            complement(713..1408)
                     /locus_tag="PLG98_12350"
     CDS             complement(713..1408)
                     /locus_tag="PLG98_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOE34103.1"
                     /translation="MTEKILSEKNRVVRHSRLELIEHWAIAISGLILLATGIFELPVA
                     KRYYIITVPGLGWTADFITSLYLHYAAALVFTAAGIFHLVYHGGARGETGLLPQKGDM
                     KASVTVIKSLFGFGQEPPMHKYLPEQRWAYVGMALIIAMLIVSGLIKTYKNLYAPDLS
                     LAIVLTATWIHNICFVLFFLAFLAHMAAILLRPNWPMVRGIFTGNVRLDYARHRHALW
                     IADLEKNKKSVDP"
     gene            complement(1401..>1795)
                     /locus_tag="PLG98_12355"
     CDS             complement(1401..>1795)
                     /locus_tag="PLG98_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOE34104.1"
                     /translation="CNDRLKEGKKPGCIEACPRGAMLIGPKKEIEAQAQARSSKINGH
                     IYGQKENGGTATLYVSPVSFELLNQTMIRKPGQPDMKPGVERRMAGTDPLGKAVLASP
                     VLGLALAGVAGWFSRRRDRKTRQEEDHD"
BASE COUNT          370 a          522 c          480 g          423 t
ORIGIN      
        1 gcttttcctt tccttcaaaa aaggccgggg cgcggttgtc gagaatgacc gcctggccga
       61 ttttacccgc aagcgaagcc ttgtccacga acagattctt gttgacggac ggcgtgaaag
      121 atttttcttt gggtctcgcc atgtcctgcg aaaccggttt gttttccttg acccaatgat
      181 tctggccgcc gttgaggatg gcgacattgg gtacgcccag gtatttcagg gtccaggcca
      241 cacgggtcat gtcgaactga tcgggaactt tgtccgtttt cccgacaatg accaccagag
      301 agttctgatc gatgcccgcg cttcccatcg cgtcggccag atcatcgatc gccggcagtt
      361 cgttcagcag gtcgcccttt ttaacggccc aggttccgta aacaatgttg acggctcccg
      421 gaatatggcc tgccttgtat tcttccagct tgcgcacatc gacgacgacc aggtttgcat
      481 tgttcaggtt cttctgcagc cactccgtgg atacaacagg atcaatgctt ctcgctgcct
      541 gtacgggagc ggtaagcgcc agcggcagca tcaacaggac cgccaggaca atcgccttca
      601 accacatcat ttttctcata acgcactacc tcctttggtt aattcattgt ttgcttttcg
      661 ctatgttgaa gcctttaacg gccctcaaac ctttccgaag cgcagctccc ggttagggat
      721 caacggattt tttgtttttt tcaagatcgg caatccacag agcatggcgg tgccgggcat
      781 agtccaggcg gacattgccg gtgaagatgc ctcgcaccat cggccagttc ggccgcagca
      841 gaatcgccgc catgtgcgcg agaaaagcca ggaaaaagag aacgaaacaa atattatgaa
      901 tccaggtggc cgtaaggacg atcgccagcg acagatccgg cgcatacagg ttcttgtagg
      961 ttttgatcag gccggacaca atcagcatcg cgatgatcaa agccattccg acgtacgccc
     1021 agcgctgttc cggcagatac ttgtgcatcg gcggttcctg gccaaatccg aaaaggctct
     1081 tgatgacggt gacggatgcc ttcatatccc ctttttgcgg cagcaaaccg gtttcgccgc
     1141 gtgctccgcc gtggtacacc agatgaaaaa ttcccgcggc cgtaaaaacc agcgccgccg
     1201 cgtaatgaag atacagggac gtgataaaat ccgccgtcca gcccagcccc ggaacggtga
     1261 tgatgtaata gcgtttggcc acgggcagtt caaagattcc ggtggccagc aaaatcaggc
     1321 cggaaatcgc gatggcccag tgttcgatca attccagccg gctgtggcgg acgacccggt
     1381 tcttctccga aaggatcttt tcagtcatgg tcttcctcct ggcgggtctt gcggtccctg
     1441 cgtcgcgaaa accagccggc cacgccggcg agcgccaatc ccagaacggg ggaagccagc
     1501 acggcttttc ccagcgggtc cgttcccgcc atgcgccgct cgacacccgg cttcatgtcc
     1561 ggctgcccgg gttttctgat catggtttga ttcaacagct cgaacgatac aggcgacaca
     1621 tagagcgtgg ccgtaccgcc gttttctttc tgaccgtaaa tgtgtccatt gatcttgctg
     1681 gagcgggcct gcgcctgggc ttctatctct tttttggggc cgatcagcat ggcgccgcgg
     1741 ggacaggctt cgatgcatcc cggctttttt ccttccttta acctgtcatt gcaga
//
LOCUS       DAOSME010000207         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_71842_length_1779_cov_19.220418, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000207 DAOSME010000000
VERSION     DAOSME010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71842_length_1779_cov_19.220418"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLG98_12360"
     CDS             complement(<1..235)
                     /locus_tag="PLG98_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34105.1"
                     /translation="MAIFEYGEYSNVYEPPDNLVDFFESSAAKYTNNPLIGEKDSAGV
                     YQWVTYGDIARRVDHLRAGLASIGVGAKDTVGII"
     gene            complement(498..1481)
                     /locus_tag="PLG98_12365"
     CDS             complement(498..1481)
                     /locus_tag="PLG98_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOE34106.1"
                     /translation="MLKKYEVMSEQVNLTKTLMAKSVVDRYLNRTNLICYSELSRLIG
                     CEAFIKHENHNPTGSFKIRGALNFFHHMSREELETGILVATRGNHGLAMAWAGQWFNV
                     PCTVVVPEGNNPEMNTTIESYGAEVIVHGGDFYDAQSYCEELVDTAGYYYVQQGNEPE
                     ILNGLATMGLEILEDLPDVDTIVCPIGGGAGCASILKTVRAFRSDIEIIGVEPQNVPS
                     FYKSWKSGEIVTLDEAHTVADGLAARTVFHLPYVIMRDAINDVVLLSEEEILEGVRMA
                     LTTTHNLAECAGAVALMAAYKIKDRLAGKKVAMIMSGGNMNMEILKQALQG"
     gene            complement(1498..>1779)
                     /locus_tag="PLG98_12370"
     CDS             complement(1498..>1779)
                     /locus_tag="PLG98_12370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE34107.1"
                     /translation="GGQNILEAAAWGKVIFYGPSMEDFSAEKDLLEEAGCGMTIRSEE
                     ELLNGIRRLLADPEEMKSRGEKGRSVVLDNIGASARYADLISRYVEADV"
BASE COUNT          416 a          512 c          392 g          459 t
ORIGIN      
        1 caatgatacc gactgtatct ttggcaccca cgccgatgga cgccaacccg gccctcaaat
       61 gatccaccct ccgggcaata tcaccgtagg tgacccattg ataaactccg gcactgtctt
      121 tttctccgat gagcggatta ttcgtgtatt tcgccgcact ggactcaaaa aagtccacca
      181 ggttatccgg tggttcatac acattactgt attcgccata ttcaaagatc gccataaacc
      241 tattcctccc tttcggttta aatcctgaac tgatacaaat ccggtacgtt atgggttggg
      301 gttgtctggt aaacgtttaa aacgaagttt caatagaaag acgatcgcaa aaactctgtt
      361 gtcgtaaaag gatagcgccc aaaagattgc cgacactctt tgatcggctt tgcgttttgg
      421 ataagttaat tatacgggca atgactcgcc aagtcaagcc aattcgaata cctcgaattg
      481 gccgttccag aggtgcatca gccttgaagc gcctgtttca aaatttccat attcatattc
      541 ccgccggaca tgatcatcgc cacttttttg ccggccaggc gatctttgat tttataagca
      601 gccatcagcg caaccgcgcc cgcgcattcg gccagattgt gcgtggtggt caacgccatt
      661 ctcacgcctt cgagaatctc ctcttcgctc aacagcacca catcgttgat cgcatctcgc
      721 ataatcacat acgggagatg gaatacggtt cttgcggcaa gaccgtctgc aacggtgtgc
      781 gcttcatcca gagttacaat ttcaccgctt ttccaggact tgtaaaagga tggaacattt
      841 tgcggctcca cgccgatgat ttcaatatcg gaccggaacg cgcgcactgt tttcagaatg
      901 gaagcgcatc ccgcgccgcc gccgatggga cagacgatcg tatccacgtc gggcagatcc
      961 tccaggattt ccaggcccat cgtcgccagg ccgtttaaga tttccggttc attgccctgt
     1021 tgaacatagt aatacccggc cgtgtccacc agttcctcgc agtaggactg ggcatcataa
     1081 aaatcgccgc cgtgaacaat cacctctgcg ccgtaacttt caatggtcgt gttcatttcc
     1141 ggattgttgc cctccggaac cacgaccgtg cagggcacgt tgaaccattg tcccgcccag
     1201 gccatggcca gtccgtgatt gccccgggtc gccaccagaa tacccgtctc gagttcctcg
     1261 cgcgacatgt gatggaaaaa attgagcgcc cccctgattt tgaaagagcc tgtcggattg
     1321 tgattttcat gtttaataaa tgcctcgcag ccgatgagac gggaaagctc cgaataacag
     1381 atcaggtttg tccggttgag ataacggtcc acaaccgatt tggccatcag ggttttagtc
     1441 agattcacct gttcactcat gacttcgtat tttttcagca tacattcctc ctgaacatta
     1501 aacatccgcc tcaacatacc ggctgatcag atcagcgtat ctggcagaag ctccgatgtt
     1561 atccagaaca acgctcctgc ccttttcgcc gcggctcttc atttcttcgg gatcggcaag
     1621 cagcctccgt atcccgttca ataattcttc ttcgcttctg atggtcatac cgcaaccggc
     1681 ctcttccagc aaatcttttt ctgcactgaa gtcctccatc gacgggccgt aaaaaatcac
     1741 cttgccccag gctgccgctt ccagaatatt ctggccgcc
//
LOCUS       DAOSME010000208         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_79597_length_1655_cov_20.800000, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000208 DAOSME010000000
VERSION     DAOSME010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79597_length_1655_cov_20.800000"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1516)
                     /locus_tag="PLG98_12375"
     CDS             complement(50..1516)
                     /locus_tag="PLG98_12375"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOE34108.1"
                     /translation="MKNSNRKTFFMFLMAFLVAFFIVSLVGVLRSSFTSSGAPEIPAA
                     QAVPSLSGGPGTAPSSFSDLVERVKPAVVNISTSKTYKGRGGFGAPFGGSPFGDDFFD
                     RFFGDMPRREFKQRSLGSGFIISNDGYIFTNNHVVEQADKIRVKISDGKEYDAKIIGT
                     DANTDIALIKIKPDNSLPVADIGDSEKVRVGEWVVAIGNPFGLDATVTAGIVSAKGRV
                     IGAGPYDNFIQTDASINPGNSGGPLFNMEGKVIGINTAIVAQGQGIGFAIPINMAKSI
                     LADLKTKGKVTRGWLGISVQDITDDIAQNLNHKNKNGALVSDVFKGDPADKAGIKIGD
                     IIIEINGKPIKDTHDLLLTIAALQVGQKMNVRAIRDGKEMTFQVVVAERKDNVTVAME
                     RSGLGHFGIAAQDITPEMAKQLGIARDGVIVTDVQAGSPADEVGIQPRDIIVQVNRVK
                     IASMKDYTREITKAAQKKSVTLFIKRGRSGYSVTIRIN"
BASE COUNT          376 a          441 c          390 g          448 t
ORIGIN      
        1 tataaaaaag gcaggcccga aggcctgcct ttttccgaag gacggatctt taattgatgc
       61 ggatcgttac ggaataaccg gatcgacccc gcttgataaa gagcgtgacg ctttttttct
      121 gagccgcttt cgtgatttca cgggtgtagt ctttcatcga cgctatttta accctgttta
      181 cctgaacgat gatgtcccgg ggttgaatgc cgacctcatc ggccggactt cccgcctgaa
      241 cgtccgtaac tataacgcca tcgcgcgcta ttccaagctg ttttgccatc tccggagtga
      301 tgtcctgcgc ggcaatgcca aaatgcccaa gcccggacct ttccatggcc acagttacat
      361 tgtctttgcg ctccgccact accacctgga aagtcatttc cttgccgtcg cgaatggctc
      421 ttacattcat cttctggcca acctgcaggg cggcaattgt caggagcaaa tcatgcgtgt
      481 ctttaatggg ctttccatta atttctataa tgatatcgcc tattttaatc ccagccttgt
      541 ctgccgggtc gcctttgaag acatccgata ccaacgcgcc gtttttgttt ttatggttta
      601 aattctgggc gatatcgtcg gtaatatcct gcacggatat tcccagccag ccgcgtgtca
      661 cctttccttt tgttttcaaa tccgcaagga tgcttttggc catattgata ggaatggcaa
      721 agccgattcc ctggccctgc gctacgatgg ccgtattgat gccgatgact ttcccttcca
      781 tattaaacag cggaccgccg ctgttgccgg gattgatcga ggcgtccgtt tgaataaaat
      841 tatcataagg tccggcgccg atgacccgtc ccttggcgct gacaatgccc gcggttaccg
      901 tcgcgtccaa accgaaagga ttgccgatcg cgaccaccca ttccccgact cgaacctttt
      961 ccgaatctcc gatatccgcc accggcaggc tgttgtccgg tttaatttta atcagagcaa
     1021 tatccgtgtt ggcgtccgtg ccgatgattt tggcgtcgta ttccttgccg tcggatattt
     1081 ttacgcggat tttgtccgcc tgttccacca cgtgattatt ggtgaagata taaccgtcat
     1141 tgctgatgat aaaccctgat cccaggctgc gctgcttgaa ttctctgcgg ggcatgtcgc
     1201 cgaaaaagcg gtcaaaaaaa tcatccccga aaggagatcc gccgaaaggc gctccaaaac
     1261 cgcctctgcc cttataggtc ttggatgtgc tgatattgac gactgccggc ttgacgcgct
     1321 ccaccagatc ggaaaacgag gagggtgccg tgccggggcc gcccgaaagg gatggaaccg
     1381 cctgcgcggc gggaatttca ggagccccgg agctggtaaa ggacgatctc agaacgccca
     1441 ccagtgaaac aataaaaaag gccaccagaa aagccatcag gaacataaaa aatgtttttc
     1501 gatttgaatt tttcatcttt tcctccaaaa gaatgcgtcc ggtgatacat ttcagatgtt
     1561 ttttaacgca taattgtttc aaatataacg acacgcctct gtttgtcaac gtaaaacgca
     1621 tttataatga tggtttgaag tattgtttgt cctgt
//
LOCUS       DAOSME010000209         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_84504_length_1586_cov_21.301764, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000209 DAOSME010000000
VERSION     DAOSME010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84504_length_1586_cov_21.301764"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLG98_12380"
     CDS             <1..526
                     /locus_tag="PLG98_12380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE34109.1"
                     /translation="DLKAVIETSKGTINLKLFADQTPVTVGNFVNLARRGYYDGLKFH
                     RVIPDFMIQGGCPFGDGRGGPGYRFQDEFVKELRHNKSGILSMANAGPQTNGSQFFIT
                     HVDTPWLDDKHTVFGEVVSDADQAVVNKIAQGDKIISVKIEGDVAPLLEKIKPALDKW
                     NTALDENFPKLAKV"
     gene            547..>1586
                     /locus_tag="PLG98_12385"
     CDS             547..>1586
                     /locus_tag="PLG98_12385"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOE34110.1"
                     /translation="MRKVTETPSKMVEKDGTIHFGTFRTPFRDVNILDVPLYSSSSGV
                     PAFWKKFRLKEWQHFGIITSNYYFGMVIFDAKFMGVSFFYAYDRNENKRFEHSVQLPG
                     GAANVAAQVYDGHCEFKKKGYRLYIENRLQQGYHKIIMDIDGKKDLPAVQGEIKVLED
                     LNGIEPLVQVSPVTPFRPFYTHKVVAPAEGEIMLGRDSIKINREWDVALMDEQKTYYP
                     YVSFWKWATAAGYNEYGELLAFNLCQNMITDDGNFNENCMWVDGKIYCLNAARFKFDE
                     QNVLHPWEVKTNDGCMDLIFTPSGERAEKISIGLIGSDFHQPFGLYNGRFKDNNNRIY
                     PITDFFGLAEHHV"
BASE COUNT          454 a          341 c          402 g          389 t
ORIGIN      
        1 tgatttaaaa gcggttattg aaaccagcaa ggggacaatc aatttaaaac tatttgcaga
       61 tcagacgccg gttacggtgg gaaattttgt caacctggcg aggcgcggtt attacgatgg
      121 tttaaaattt catcgggtaa tcccagattt catgattcag ggcggatgtc catttggcga
      181 cggccgcggt ggacccggat acagatttca agacgaattt gtcaaagaat tgcgtcacaa
      241 taagtccggt attctgtcga tggccaacgc cgggccgcag accaacggca gccagttttt
      301 catcacgcac gtggataccc cgtggctgga cgacaaacac acggtattcg gtgaagtggt
      361 cagcgatgcc gatcaggctg tggtcaacaa aatcgcccag ggcgataaaa tcatctccgt
      421 gaaaattgaa ggagatgttg cgccgctgtt ggaaaaaatc aaaccggcac tggataaatg
      481 gaatactgcc ctggatgaaa atttcccgaa gcttgcgaag gtttaatgca ataaacaaga
      541 cgcgatatga gaaaagtaac agaaactccc tcaaagatgg tggaaaaaga cggaacgatt
      601 catttcggga cgtttcgcac gccttttcgc gatgtgaata ttcttgacgt gccgctttat
      661 tcgtcttcat ccggcgttcc cgcattctgg aagaaattcc gtctcaagga atggcagcat
      721 ttcggtatca tcacgtccaa ctattatttt ggcatggtga tctttgacgc caagtttatg
      781 ggagtctcct ttttttatgc ctacgacaga aatgagaata agcgtttcga acacagtgtg
      841 caattaccgg gcggtgcggc gaatgttgct gcacaggttt atgatggcca ctgcgaattc
      901 aaaaaaaaag gttaccgact ttatattgaa aacagattgc aacagggcta tcataaaatc
      961 atcatggaca tcgacggcaa aaaagatctg ccggctgttc aaggggaaat taaagttctg
     1021 gaagacctca atgggataga accgctggtg caggtttctc cggtcacgcc tttccgtcct
     1081 ttttacacgc ataaggttgt tgcgccggcg gaaggtgaaa ttatgctggg cagggatagc
     1141 atcaagatta accgggaatg ggatgtcgcc ctgatggacg aacagaaaac atattaccct
     1201 tatgtcagtt tctggaaatg ggcgacggcc gcggggtaca acgaatacgg agagctcctc
     1261 gcttttaatc tctgtcagaa catgattacc gatgacggga atttcaacga aaactgcatg
     1321 tgggtggacg gaaaaatata ctgcctcaac gcggcgcgat ttaaattcga tgaacagaat
     1381 gtcctgcatc cctgggaagt caaaacgaat gatggatgca tggatttaat atttacgcct
     1441 tccggtgaaa gagccgaaaa aatcagcatc gggctgatcg gatccgattt tcaccagccg
     1501 ttcggcctct ataacggacg attcaaggac aacaacaata gaatatatcc gattaccgat
     1561 tttttcggat tggccgagca tcacgt
//
LOCUS       DAOSME010000210         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_1605
            NODE_87838_length_1542_cov_16.226631, whole genome shotgun
            sequence.
ACCESSION   DAOSME010000210 DAOSME010000000
VERSION     DAOSME010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863134
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:44:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,480
            CDSs (total)                      :: 2,462
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87838_length_1542_cov_16.226631"
                     /isolate="AMR_MDS_1605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLG98_12390"
     CDS             <1..250
                     /locus_tag="PLG98_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE34111.1"
                     /translation="KDHIEKPYDEKVQILRAFHDHLFAEYSKILSGPAHITNKMKEFW
                     TYSGSFLKDREKIRKQINKTHHRDAYIDAVHKIFEESV"
     gene            complement(306..>1056)
                     /locus_tag="PLG98_12395"
     CDS             complement(306..>1056)
                     /locus_tag="PLG98_12395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE34112.1"
                     /translation="DAECFYAADPQGFFLAEADGQPAGCLSAVAYDEHFGFAGFYIVL
                     PSFRNLGIGHELVRTASAYAGSRSMGNDAVVAQQETYRQLGFSLAYRNIRYRGLASPE
                     TTDASGVIDLQRIPFRDLVVYDRGMFPAERPVFLQSWIHQSEATALGCEDNGRLTGYG
                     VIRKCRRGYKIGPLFADREDIAERLFQALTGRIPGEEFFLDIPEPNAAAGSLVRRHNM
                     QSVFETARMYSGTVPPLPLDRIFGVTSFELG"
     assembly_gap    1057..1124
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1279..>1542
                     /locus_tag="PLG98_12400"
     CDS             1279..>1542
                     /locus_tag="PLG98_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE34113.1"
                     /translation="MRKETGHARFGSVLVIPGEDGCRFPYCTSVLIDDDVRVLVDPGA
                     GLQALVSLQKKAPVDLVINTHYHFDHIAYNYLFDRARILLNEQE"
BASE COUNT          344 a          382 c          411 g          337 t
ORIGIN      
        1 caaggaccat attgaaaaac cgtatgacga aaaagtccaa atcctgcggg cgtttcacga
       61 tcacctgttt gccgaatatt caaaaattct gtccggcccc gcgcatatca caaataaaat
      121 gaaggagttc tggacgtatt cggggagttt tttgaaagac agggaaaaaa tccgcaagca
      181 aatcaataaa acccatcacc gcgatgctta tatcgatgcc gtccataaga tatttgagga
      241 gtcggtttga cggcggatgg tttgcctgat ttccggtgca aagacttttt cgatttccca
      301 acgggctatc ccagttcaaa gctggtcacg ccgaagattc tatccagcgg caggggaggg
      361 accgtgccgg aatacatgcg ggcggtttcg aaaacactct gcatgttgtg gcggcgaacg
      421 agcgaaccgg cggccgcatt gggttcgggg atatccagaa aaaattcttc gccggggatc
      481 cggccggtca gggcctggaa taatctttcg gcaatgtctt cacggtcggc aaacagcggg
      541 ccgattttat agccccggcg gcacttgcga atcacgccgt atccggtcag tcttccattg
      601 tcctcgcaac ccagcgccgt cgcttccgac tggtggatcc aggattgcag aaaaacgggg
      661 cgttcggccg gaaacatgcc ccggtcgtaa acgaccagat cgcgaaaagg gattctttgc
      721 aggtcgatga ctcccgacgc gtctgtcgtt tcgggggaag ccagtccgcg atagcggatg
      781 ttccggtacg ccagactaaa gcccagttgc ctgtaagtct cctgctgggc gacaacggcg
      841 tcgttgccca tgctgcggct gccggcatag gcggatgccg tccggaccag ttcatgtccg
      901 attcccaggt tgcggaatga gggcaggaca atataaaacc ccgcaaaacc gaagtgctcg
      961 tcataggcca ccgcggaaag acatcccgcc ggctgtccgt ctgcttcggc caggaaaaac
     1021 ccctggggat cggccgcgta aaaacattcc gcgtcannnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcaat tggatagttg
     1141 atctttaggg tgaattctgt taccggattt tcaaacaggc ccggcggaag aagcgctttg
     1201 tgaaatgcgt cttgccgctt tctgaaattt cagggcggac agcatcttga cgccccggcc
     1261 atttttgaga atgtctttat gaggaaagaa accggacatg cgcgatttgg ttccgtcctc
     1321 gtcattccgg gagaagacgg gtgcagattt ccctattgca cgtctgtgtt gatcgatgac
     1381 gacgtcaggg ttctggttga tccgggcgcg ggccttcagg cgcttgtttc gctgcaaaag
     1441 aaagcgcccg tcgatctggt gatcaatact cattatcact tcgaccatat tgcgtataat
     1501 tacctttttg accgggcgcg gattctgctc aatgagcagg aa
//
